Sample records for fluorescent bulb compared

  1. How Energy-Efficient Light Bulbs Compare with Traditional Incandescent...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    used light fixtures or bulbs with models that have earned the ENERGY STAR, you can save 75 each year. Compared to traditional incandescents, energy-efficient lightbulbs...

  2. Frequently Asked Questions Information on Compact Fluorescent Light Bulbs (CFLs) and Mercury

    E-Print Network [OSTI]

    Jia, Songtao

    Frequently Asked Questions Information on Compact Fluorescent Light Bulbs (CFLs) and Mercury February 2008 Why should people use CFLs? Switching from traditional light bulbs to CFLs is an effective light bulbs, last up to 10 times longer, cost little up front, and provide a quick return on investment

  3. The Hazards of Use and Disposal of Compact Fluorescent Bulbs Compact fluorescent lights (aka. CFLs) work by exciting a phosphorous coating within the tube to emit

    E-Print Network [OSTI]

    Maroncelli, Mark

    The Hazards of Use and Disposal of Compact Fluorescent Bulbs Compact fluorescent lights (aka. CFLs the bulb to be an efficient light source. By comparison, older home thermometers contain 500 milligrams decade. HOW SHOULD I DISPOSE OF CFL'S? The presence of mercury requires that all CFL bulbs are properly

  4. Mercury Lamps Recycling Fluorescent light-tubes, compact fluorescent bulbs, mercury and sodium vapor lamps, ultraviolet and

    E-Print Network [OSTI]

    Baker, Chris I.

    Mercury Lamps Recycling Fluorescent light-tubes, compact fluorescent bulbs, mercury and sodium labeled for shipment to a recycling plant for mercury, glass and aluminum recovery. The beneficial re can be recycled infinitely without losing its purity or strength. While the primary end product

  5. Spatial assessment of net mercury emissions from the use of fluorescent bulbs

    SciTech Connect (OSTI)

    Matthew J. Eckelman; Paul T. Anastas; Julie B. Zimmerman [Yale University, New Haven, CT (United States). Department of Chemical Engineering

    2008-11-15T23:59:59.000Z

    While fluorescent lighting is an important technology for reducing electrical energy demand, mercury used in the bulbs is an ongoing concern. Using state and country level data, net emissions of mercury from the marginal use of fluorescent lightbulbs are examined for a base year of 2004 for each of the 50 United States and 130 countries. Combustion of coal for electric power generation is generally the largest source of atmospheric mercury pollution; reduction in electricity demand from the substitution of incandescent bulbs with fluorescents leads to reduced mercury emissions during the use of the bulb. This analysis considers the local mix of power sources, coal quality, thermal conversion efficiencies, distribution losses, and any mercury control technologies that might be in place. Emissions of mercury from production and end-of-life treatment of the bulbs are also considered, providing a life-cycle perspective. Net reductions in mercury over the entire life cycle range from -1.2 to 97 mg per bulb depending on the country. The consequences for atmospheric mercury emissions of several policy scenarios are also discussed. 46 refs., 4 figs., 3 tabs.

  6. Waste Toolkit A-Z Light bulbs

    E-Print Network [OSTI]

    Melham, Tom

    Waste Toolkit A-Z Light bulbs Can I recycle light bulbs? It depends what type of bulbs you have of in the normal University waste bins (landfill waste). Energy saving bulbs and fluorescent tubes are classified light bulbs? Standard filament bulbs Put in the waste bin (landfill waste) as these are not classified

  7. Visualization of nitric oxide production in the mouse main olfactory bulb by a cell-trappable copper(II) fluorescent probe

    E-Print Network [OSTI]

    McQuade, Lindsey E.

    We report the visualization of NO production using fluorescence in tissue slices of the mouse main olfactory bulb. This discovery was possible through the use of a novel, cell-trappable probe for intracellular nitric oxide ...

  8. Bulb mounting of solar cell

    SciTech Connect (OSTI)

    Thompson, M.E.

    1983-04-05T23:59:59.000Z

    An energy converting assembly is provided for parasiting of light from a fluorescent light bulb utilizing a solar cell. The solar cell is mounted on a base member elongated in the dimension of elongation of the fluorescent bulb, and electrical interconnections to the cell are provided. A flexible sheet of opaque material having a flat white interior reflective surface surrounds the fluorescent bulb and reflects light emitted from the bulb back toward the bulb and the solar cell. The reflective sheet is tightly held in contact with the bottom of the bulb by adhesive, a tie strap, an external clip, or the like.

  9. Consumer Light Bulb Changes: Briefing and Resources for Media...

    Broader source: Energy.gov (indexed) [DOE]

    flux") - CFL: Compact Fluorescent Lamp: The curly fluorescent bulbs - LED: Light Emitting Diode: more recently emerging technology, also called "solid state lighting" as it is...

  10. Comparing Light Bulbs

    Broader source: Energy.gov [DOE]

    In this exercise, students will use a light to demonstrate the difference between being energy-efficient and energy-wasteful, and learn what energy efficiency means.

  11. Physiological properties and factors affecting migration of neural precursor cells in the adult olfactory bulb

    E-Print Network [OSTI]

    Darcy, Daniel Paul

    2007-01-01T23:59:59.000Z

    neurons in the olfactory bulb." J Neurosci 26(41): 10508-in the olfactory bulb." The Journal of comparative neurologycells to the olfactory bulb." J Neurosci 21(21): 8505-13.

  12. Jacketed lamp bulb envelope

    DOE Patents [OSTI]

    MacLennan, Donald A. (Gaithersburg, MD); Turner, Brian P. (Damascus, MD); Gitsevich, Aleksandr (Gaithersburg, MD); Bass, Gary K. (Mt. Airy, MD); Dolan, James T. (Frederick, MD); Kipling, Kent (Gaithersburg, MD); Kirkpatrick, Douglas A. (Great Falls, VA); Leng, Yongzhang (Damascus, MD); Levin, Izrail (Silver Spring, MD); Roy, Robert J. (Frederick, MD); Shanks, Bruce (Gaithersburg, MD); Smith, Malcolm (Alexandria, VA); Trimble, William C. (Columbia, MD); Tsai, Peter (Olney, MD)

    2001-01-01T23:59:59.000Z

    A jacketed lamp bulb envelope includes a ceramic cup having an open end and a partially closed end, the partially closed end defining an aperture, a lamp bulb positioned inside the ceramic cup abutting the aperture, and a reflective ceramic material at least partially covering a portion of the bulb not abutting the aperture. The reflective ceramic material may substantially fill an interior volume of the ceramic cup not occupied by the bulb. The ceramic cup may include a structural feature for aiding in alignment of the jacketed lamp bulb envelope in a lamp. The ceramic cup may include an external flange about a periphery thereof. One example of a jacketed lamp bulb envelope includes a ceramic cup having an open end and a closed end, a ceramic washer covering the open end of the ceramic cup, the washer defining an aperture therethrough, a lamp bulb positioned inside the ceramic cup abutting the aperture, and a reflective ceramic material filling an interior volume of the ceramic cup not occupied by the bulb. A method of packing a jacketed lamp bulb envelope of the type comprising a ceramic cup with a lamp bulb disposed therein includes the steps of filling the ceramic cup with a flowable slurry of reflective material, and applying centrifugal force to the cup to pack the reflective material therein.

  13. Wiring Switches to Light Bulbs

    E-Print Network [OSTI]

    Buckley, Stephen M

    2011-01-01T23:59:59.000Z

    Given n buttons and n bulbs so that the ith button toggles the ith bulb and at most two other bulbs, we compute the sharp lower bound on the number of bulbs that can be lit regardless of the action of the buttons.

  14. Solar light bulb

    SciTech Connect (OSTI)

    Smith, D.A.

    1983-07-26T23:59:59.000Z

    A system for generating light directly using solar energy is provided herein. It includes a concentrator and accumulator for the sun's rays to generate a concentrated beam of visible solar radiation. A distributor shaft is provided for distributing the beam of visible solar radiation. A fork is provided in the distributor shaft to define a plurality of branch lines, each provided with a mirror at the intersection to direct the beam down the respective branch line to permit parallel fractions of the beam to be reflected off the respective mirrors and to pass down the respective branch line. A solar bulb is provided including a double walled upper bulbous portion including the inlet from the branch line and a pair of heat outlet tubes, and a double walled lower bulbous portion, the upper portion thereof being divergently reflective, with the lower portion having walls which are either transparent or translucent to provide greater light diffusion, and the space between the two walls being maintained under vacuum to provide heat insulation values. A structure is provided within the solar bulb for the absorption and radiation of the concentrated beam of visible solar radiation. Preferably structure is provided connected to the solar bulb to draw in outside air in the summer to direct it past the solar bulb and to air vent hot air produced at the solar bulb to the outside, thereby providing light with minimal heat in the summer. The same structure is operated in the winter to draw in household air to direct it past the solar bulb and to recirculate such heated air produced at the solar bulb to the house, thereby providing light and heat in the winter.

  15. Lamp bulb with integral reflector

    DOE Patents [OSTI]

    Levin, Izrail (Silver Spring, MD); Shanks, Bruce (Gaithersburg, MD); Sumner, Thomas L. (Wheaton, MD)

    2001-01-01T23:59:59.000Z

    An improved electrodeless discharge lamp bulb includes an integral ceramic reflector as a portion of the bulb envelope. The bulb envelope further includes two pieces, a reflector portion or segment is cast quartz ceramic and a light transmissive portion is a clear fused silica. In one embodiment, the cast quartz ceramic segment includes heat sink fins or stubs providing an increased outside surface area to dissipate internal heat. In another embodiment, the quartz ceramic segment includes an outside surface fused to eliminate gas permeation by polishing.

  16. Dendritic processing within olfactory bulb circuits

    E-Print Network [OSTI]

    Betz, William J.

    Dendritic processing within olfactory bulb circuits Nathan E. Schoppa1 and Nathan N. Urban2 1 of the olfactory bulb. How- ever, the mechanisms by which this map is transformed into an odor code by the bulb circuitry remain unclear. Recent physiological studies in bulb slices have ident- ified several synaptic

  17. Stochastic modeling of hourly dry-bulb and wet-bulb temperatures

    SciTech Connect (OSTI)

    Kline, D.E.; Woeste, F.E.

    1984-01-01T23:59:59.000Z

    A stochastic model of hourly dry-bulb and wet-bulb temperatures was developed. The periodic variations over the course of a year were estimated by least-square approximation. A multisite Markov model was used to simulate the stochastic nature of the data. These two models were combined to simulate years of hourly dry-bulb and wet-bulb temperatures.

  18. Fooling Mother Nature: Forcing Flower Bulbs

    E-Print Network [OSTI]

    Liskiewicz, Maciej

    Fooling Mother Nature: Forcing Flower Bulbs for Indoor Bloom by George Graine,Virginia Cooperative Bulbs for Indoor Bloom George Graine,Virginia Cooperative Extension Master Gardener, Fairfax County, VA Introduction Have you ever wondered if it is possible to enjoy the beauty of bulbs in the middle of winter

  19. (bulb) , (1) Gas(1) Gas(1) Gas(1) Gas----saturation methodsaturation methodsaturation methodsaturation method

    E-Print Network [OSTI]

    Hong, Deog Ki

    (bulb) , . . , . . 1. . (1) Gas(1Static MethodStatic Method Isoteniscope bulb U-tube . bulb U-tube bulb . bulb . U bulb . manometer . . Isoteniscope Boling Point Method . #12;2. (1) Boiling

  20. Type of Space Bulb Type #/House Fixture Style Greenhouse #

    E-Print Network [OSTI]

    Pawlowski, Wojtek

    Type of Space Bulb Type #/House Fixture Style Greenhouse # 1 Lu 430/Lu 400 24 White box style 2 Lu No bulbs 0 N/A Seed harvest room F32 T8/TL 841 90 bulbs VIGS Room F032 /741/ECO 60 bulbs Chamber Model Bulb

  1. Consumer Light Bulb Changes: Briefing and Resources for Media...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Consumer Light Bulb Changes: Briefing and Resources for Media and Retailers Consumer Light Bulb Changes: Briefing and Resources for Media and Retailers This presentation provides...

  2. The New York City Bulb Garden [Transforming the American Garden: 12 New Landscape Designs-Exhibitor

    E-Print Network [OSTI]

    Schwartz, Martha

    1986-01-01T23:59:59.000Z

    point of intersection. The bulbs selected for this schemefall and late fall. The bulbs have been selected for theiris designed for flexibility. Bulbs can be replaced and new

  3. Magnetic fluorescent lamp

    DOE Patents [OSTI]

    Berman, S.M.; Richardson R.W.

    1983-12-29T23:59:59.000Z

    The radiant emission of a mercury-argon discharge in a fluorescent lamp assembly is enhanced by providing means for establishing a magnetic field with lines of force along the path of electron flow through the bulb of the lamp assembly, to provide Zeeman splitting of the ultraviolet spectral line. Optimum results are obtained when the magnetic field strength causes a Zeeman splitting of approximately 1.7 times the thermal line width.

  4. 100 Prisoners and A light Bulb Yisong Song

    E-Print Network [OSTI]

    Morrow, James A.

    100 Prisoners and A light Bulb Yisong Song 1. Introduction The article represent three different protocols for solving the "100 Prisoners and a Light Bulb" riddle, including the explicit computations only a light bulb with a toggle switch. The prisoner will be able to observe the current state

  5. The Somatotopic Organization of the Olfactory Bulb in Elasmobranchs

    E-Print Network [OSTI]

    Kajiura, Stephen

    The Somatotopic Organization of the Olfactory Bulb in Elasmobranchs Tricia L. Meredith,1, University of Colorado Denver, Anschutz Medical Campus, Aurora, Colorado 80045 ABSTRACT The olfactory bulbs-olfac- tory bulbs.'' Somatotopy could provide a preadaptation which facilitated the evolution of olfactory

  6. Cellular/Molecular Detecting Activity in Olfactory Bulb Glomeruli with

    E-Print Network [OSTI]

    Newman, Eric A.

    Cellular/Molecular Detecting Activity in Olfactory Bulb Glomeruli with Astrocyte Recording Didier, Oregon 97239 In the olfactory bulb, axons of olfactory sensory neurons (OSNs) expressing the same to examine functional compartmentalization within and between olfactory bulb glomeruli. Key words: olfactory

  7. BULB: Onion-Based Measuring of OSS Communities

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    BULB: Onion-Based Measuring of OSS Communities Terhi Kilamo1 , Timo Aaltonen1 , and Teemu J. Heinim based on scarce information. In this paper a measuring model for open source commu- nities, BULB, is introduced. BULB provides a way of collecting relevant information and relates it to the well-known onion

  8. Encoding Pheromonal Signals in the Accessory Olfactory Bulb of

    E-Print Network [OSTI]

    Fee, Michale S.

    Encoding Pheromonal Signals in the Accessory Olfactory Bulb of Behaving Mice Minmin Luo,1 * Michale single neurons in the accessory olfactory bulb, a nucleusthatprocessespheromonalsignals with sources of pheromones (4, 5). Olfactory receptor neurons project to the main olfactory bulb (MOB), whose

  9. Chemical Dependencies of Learning in the Rabbit Olfactory Bulb: Acquisition of the Transient Spatial Pattern Chance Depends on Norepinephrine

    E-Print Network [OSTI]

    Gray, Charles M; Freeman, Walter J III; Skinner, James E

    1986-01-01T23:59:59.000Z

    of neurons in the olfactory bulb following prolonged odorforcbrain (3): Olfactory bulb. anterior olfactory nucleus.39;thc rat olfactory bulb (2): Fluorescnce histochemical.

  10. Jacob P. Brenner Design Specifications for Wet-Bulb Aspirator Apparatus

    E-Print Network [OSTI]

    Wisconsin at Madison, University of

    Jacob P. Brenner Thesis: Design Specifications for Wet-Bulb Aspirator Apparatus Project Description the concentration of water vapor in the air is the wet-bulb temperature. The wet-bulb temperature along with the dry-bulb for the testing of HVAC&R equipment. The focus of this research is on the accurate measurement of the wet-bulb

  11. SuperBulbs Inc | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directedAnnualProperty Edit with formSoutheastern ILSunseeker Energy Holding AG JumpSunways AGSuperBulbs Inc

  12. Modeling the wet bulb globe temperature using standard meteorological measurements.

    SciTech Connect (OSTI)

    Liljegren, J. C.; Carhart, R. A.; Lawday, P.; Tschopp, S.; Sharp, R.; Decision and Information Sciences

    2008-10-01T23:59:59.000Z

    The U.S. Army has a need for continuous, accurate estimates of the wet bulb globe temperature to protect soldiers and civilian workers from heat-related injuries, including those involved in the storage and destruction of aging chemical munitions at depots across the United States. At these depots, workers must don protective clothing that increases their risk of heat-related injury. Because of the difficulty in making continuous, accurate measurements of wet bulb globe temperature outdoors, the authors have developed a model of the wet bulb globe temperature that relies only on standard meteorological data available at each storage depot for input. The model is composed of separate submodels of the natural wet bulb and globe temperatures that are based on fundamental principles of heat and mass transfer, has no site-dependent parameters, and achieves an accuracy of better than 1 C based on comparisons with wet bulb globe temperature measurements at all depots.

  13. How Energy-Efficient Light Bulbs Compare with Traditional Incandescents |

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742 33Frequently20,000 RussianBy: Thomas P. D'Agostino,GlenLearningDepartmentDistributed

  14. Heat transfer assembly for a fluorescent lamp and fixture

    DOE Patents [OSTI]

    Siminovitch, M.J.; Rubenstein, F.M.; Whitman, R.E.

    1992-12-29T23:59:59.000Z

    In a lighting fixture including a lamp and a housing, a heat transfer structure is disclosed for reducing the minimum lamp wall temperature of a fluorescent light bulb. The heat transfer structure, constructed of thermally conductive material, extends from inside the housing to outside the housing, transferring heat energy generated from a fluorescent light bulb to outside the housing where the heat energy is dissipated to the ambient air outside the housing. Also disclosed is a method for reducing minimum lamp wall temperatures. Further disclosed is an improved lighting fixture including a lamp, a housing and the aforementioned heat transfer structure. 11 figs.

  15. Heat transfer assembly for a fluorescent lamp and fixture

    SciTech Connect (OSTI)

    Siminovitch, Michael J. (Richmond, CA); Rubenstein, Francis M. (Berkeley, CA); Whitman, Richard E. (Richmond, CA)

    1992-01-01T23:59:59.000Z

    In a lighting fixture including a lamp and a housing, a heat transfer structure is disclosed for reducing the minimum lamp wall temperature of a fluorescent light bulb. The heat transfer structure, constructed of thermally conductive material, extends from inside the housing to outside the housing, transferring heat energy generated from a fluorescent light bulb to outside the housing where the heat energy is dissipated to the ambient air outside the housing. Also disclosed is a method for reducing minimum lamp wall temperatures. Further disclosed is an improved lighting fixture including a lamp, a housing and the aforementioned heat transfer structure.

  16. A new maintenance friendly concept for bulb type generators

    SciTech Connect (OSTI)

    Scheidl, W.

    1998-07-01T23:59:59.000Z

    Bulb type generators in the rating range up to 15 MW are in a severe competition with geared up machines which operate at much higher speed and are therefore smaller and cheaper. Up to now the conventional bulb type generator in the standard design as it is used on several rivers in Austria and many other countries was not so economic in the lower rating range. A new and maintenance friendly concept for the bulb type generator design especially for these lower rating range ended in a more economic solution and is now able to compete with the geared up machines.

  17. Fluorescent optical position sensor

    DOE Patents [OSTI]

    Weiss, Jonathan D.

    2005-11-15T23:59:59.000Z

    A fluorescent optical position sensor and method of operation. A small excitation source side-pumps a localized region of fluorescence at an unknown position along a fluorescent waveguide. As the fluorescent light travels down the waveguide, the intensity of fluorescent light decreases due to absorption. By measuring with one (or two) photodetectors the attenuated intensity of fluorescent light emitted from one (or both) ends of the waveguide, the position of the excitation source relative to the waveguide can be determined by comparing the measured light intensity to a calibrated response curve or mathematical model. Alternatively, excitation light can be pumped into an end of the waveguide, which generates an exponentially-decaying continuous source of fluorescent light along the length of the waveguide. The position of a photodetector oriented to view the side of the waveguide can be uniquely determined by measuring the intensity of the fluorescent light emitted radially at that location.

  18. Changes in neurotransmitter levels and proinflammatory cytokine mRNA expressions in the mice olfactory bulb following nanoparticle exposure

    SciTech Connect (OSTI)

    Tin-Tin-Win-Shwe [National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki 305-8506 (Japan)], E-mail: tin.tin.win.shwe@nies.go.jp; Mitsushima, Dai [Department of Physiology, Yokohama City University Graduate School of Medicine, 3-9 Fukuura, Kanazawaku, Yokohama 236-0004 (Japan); Yamamoto, Shoji; Fukushima, Atsushi [National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki 305-8506 (Japan); Funabashi, Toshiya [Department of Physiology, Yokohama City University Graduate School of Medicine, 3-9 Fukuura, Kanazawaku, Yokohama 236-0004 (Japan); Kobayashi, Takahiro; Fujimaki, Hidekazu [National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki 305-8506 (Japan)

    2008-01-15T23:59:59.000Z

    Recently, there have been increasing reports that nano-sized component of particulate matter can reach the brain and may be associated with neurodegenerative diseases. Previously, our laboratory has studied the effect of intranasal instillation of nano-sized carbon black (CB) (14 nm and 95 nm) on brain cytokine and chemokine mRNA expressions and found that 14-nm CB increased IL-1{beta}, TNF-{alpha}, CCL2 and CCL3 mRNA expressions in the olfactory bulb, not in the hippocampus of mice. To investigate the effect of a single administration of nanoparticles on neurotransmitters and proinflammatory cytokines in a mouse olfactory bulb, we performed in vivo microdialysis and real-time PCR methods. Ten-week-old male BALB/c mice were implanted with guide cannula in the right olfactory bulb and, 1 week later, were instilled vehicle or CB (14 nm, 250 {mu}g) intranasally. Six hours after the nanoparticle instillation, the mice were intraperitoneally injected with normal saline or 50 {mu}g of bacteria cell wall component lipoteichoic acid (LTA), which may potentiate CB-induced neurologic effect. Extracellular glutamate and glycine levels were significantly increased in the olfactory bulb of CB-instilled mice when compared with vehicle-instilled control mice. Moreover, we found that LTA further increased glutamate and glycine levels. However, no alteration of taurine and GABA levels was observed in the olfactory bulb of the same mice. We also detected immunological changes in the olfactory bulb 11 h after vehicle or CB instillation and found that IL-1{beta} mRNA expression was significantly increased in CB- and LTA-treated mice when compared with control group. However, TNF-{alpha} mRNA expression was increased significantly in CB- and saline-treated mice when compared with control group. These findings suggest that nanoparticle CB may modulate the extracellular amino acid neurotransmitter levels and proinflammatory cytokine IL-1 {beta} mRNA expressions synergistically with LTA in the mice olfactory bulb.

  19. Radiation Dose-Volume Effects and the Penile Bulb

    SciTech Connect (OSTI)

    Roach, Mack, E-mail: mroach@radonc.ucsf.ed [Department of Radiation Oncology, University of California-San Francisco, San Francisco, CA (United States); Nam, Jiho [Department of Radiation Oncology, University of North Carolina at Chapel Hill, Chapel Hill, NC (United States); Gagliardi, Giovanna [Department of Medical Physics, Karolinska University Hospital and Karolinska Institutet, Stockholm (Sweden); El Naqa, Issam; Deasy, Joseph O. [Department of Radiation Oncology, School of Medicine, Washington University in St. Louis, St. Louis, MO (United States); Marks, Lawrence B. [Department of Radiation Oncology, University of North Carolina at Chapel Hill, Chapel Hill, NC (United States)

    2010-03-01T23:59:59.000Z

    The dose, volume, and clinical outcome data for penile bulb are reviewed for patients treated with external-beam radiotherapy. Most, but not all, studies find an association between impotence and dosimetric parameters (e.g., threshold doses) and clinical factors (e.g., age, comorbid diseases). According to the data available, it is prudent to keep the mean dose to 95% of the penile bulb volume to <50 Gy. It may also be prudent to limit the D70 and D90 to 70 Gy and 50 Gy, respectively, but coverage of the planning target volume should not be compromised. It is acknowledged that the penile bulb may not be the critical component of the erectile apparatus, but it seems to be a surrogate for yet to be determined structure(s) critical for erectile function for at least some techniques.

  20. Text-Alternative Version: L Prize™: The Race for Super Efficient Light Bulbs

    Broader source: Energy.gov [DOE]

    Below is the text-alternative version of the L Prize™: The Race for Super Efficient Light Bulbs webcast.

  1. Modlisation des rythmes rapides bta et gamma du bulbe olfactif Nicolas Fourcaud-Trocm

    E-Print Network [OSTI]

    Boyer, Edmond

    Modélisation des rythmes rapides bêta et gamma du bulbe olfactif Nicolas Fourcaud-Trocmé Université mammifères, la première zone corticale traitant l'information olfactive est le bulbe olfactif (BO). Le codage anesthésié. MOTS-CLES bulbe olfactif, oscillations, bêta, gamma, modèle 1. Introduction En neurosciences, les

  2. Onion ipmPIPE -Diagnostic Pocket Series Bulb Growth Stages of Onion (Allium cepa L.)

    E-Print Network [OSTI]

    Pappu, Hanu R.

    Onion ipmPIPE - Diagnostic Pocket Series Bulb Growth Stages of Onion (Allium cepa L.) Fig 1 Fig 2 Fig 3 Fig 4 Fig 5 Fig 6 Fig 7 Fig 8 Allium Type: Fresh Market and Storage Onion Both pre- and post-bulb planting (seeds, transplants, sets). Pre-Bulb Growth Stages 1 ­ radical and flag leaf emergence (10-30 days

  3. Amlioration des plantes Production de bulbes in vitro et de novo

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    Amélioration des plantes Production de bulbes in vitro et de novo à partir de tissus de tiges à bulbes, 29260 Ploudaniel; 2 Université Blaise Pascal, laboratoire de physiologie et génétique décembre 1992) Résumé — L'obtention de novo de bulbes in vitro est possible chez la tulipe dans un

  4. Behavioral/Systems/Cognitive Olfactory Bulb Gamma Oscillations Are Enhanced with

    E-Print Network [OSTI]

    Kopell, Nancy

    Behavioral/Systems/Cognitive Olfactory Bulb Gamma Oscillations Are Enhanced with Task Demands.However,thereisstillnodirectevidencethatsuchoscillationsareintrinsicallyalteredinintactsystems to aid in stimulus disambiguation. Here we show that gamma oscillatory power in the rat olfactory bulb; sensory acuity; beta oscillations; olfactory bulb; piriform (pyriform) Introduction The chemotopic

  5. Dendrodendritic Inhibition in the Olfactory Bulb Is Driven by NMDA Receptors

    E-Print Network [OSTI]

    Betz, William J.

    Dendrodendritic Inhibition in the Olfactory Bulb Is Driven by NMDA Receptors Nathan E. Schoppa,1 J synapses between mitral and granule cells in the rat olfactory bulb. In whole-cell patch-clamp recordings in bulb slices, stimulation of mitral cells elicited slowly decaying, GABAA receptor-mediated reciprocal

  6. THE LIGHT BULB PROBLEM 1 Ramamohan Paturi2 Sanguthevar Rajasekaran3 John Reif3

    E-Print Network [OSTI]

    Rajasekaran, Sanguthevar

    THE LIGHT BULB PROBLEM 1 Ramamohan Paturi2 Sanguthevar Rajasekaran3 John Reif3 Univ. of California Title: The Light Bulb Problem Corresponding Author: Ramamohan Paturi Department of Computer Science Mail correlational learning problem due to L. G. Valiant, 1985 and 1988: We have a sequence of n random light bulbs

  7. ACTION DE L'ABLATION DES BULBES OLFACTIFS SUR LES MCANISMES DE LA REPRODUCTION

    E-Print Network [OSTI]

    Boyer, Edmond

    ACTION DE L'ABLATION DES BULBES OLFACTIFS SUR LES MÉCANISMES DE LA REPRODUCTION CHEZ LA TRUIE J. P Recheyches zoolechniques, Jouy-en-Josas (Seine et Oise). SOMMAIRE L'ablation chirurgicale des bulbes involution utérine. L'ablation totale des bulbes olfactifs semble donc perturber la décharge des hormones

  8. STABLE FREQUENCY RESPONSE TO VARYING STIMULUS INTENSITY IN A MODEL OF THE RAT OLFACTORY BULB

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    STABLE FREQUENCY RESPONSE TO VARYING STIMULUS INTENSITY IN A MODEL OF THE RAT OLFACTORY BULB Thomas Neurosciences et Systèmes sensoriels, CNRS UMR 5020, Lyon, France ABSTRACT In the rat olfactory bulb (OB), fast. Introduction The rat olfactory bulb (OB) is a rich and complex sensory processing system that shows stimulus

  9. THE LIGHT BULB PROBLEM 1 Ramamohan Paturi2 Sanguthevar Rajasekaran3 John Reif3

    E-Print Network [OSTI]

    Rajasekaran, Sanguthevar

    THE LIGHT BULB PROBLEM 1 Ramamohan Paturi2 Sanguthevar Rajasekaran3 John Reif3 Univ. of California, San Diego Univ. of Pennsylvania Duke University 1 #12;Running Title: The Light Bulb Problem, 1985 and 1988: We have a sequence of n random light bulbs each of which is either on or off with equal

  10. Editorial Focus Fish Smell. Focus on "Odorant Specificity of Single Olfactory Bulb Neurons

    E-Print Network [OSTI]

    Stephens, Jacqueline

    Editorial Focus Fish Smell. Focus on "Odorant Specificity of Single Olfactory Bulb Neurons to Amino bulb, or antennal lobe. The precise nature of these odor features is yet to be determined for the most of this type in the fish, precise subclasses of olfactory bulb neurons for coding biologically significant

  11. Dendrodendritic Inhibition and Simulated Odor Responses in a Detailed Olfactory Bulb Network Model

    E-Print Network [OSTI]

    Feng, Jianfeng

    Dendrodendritic Inhibition and Simulated Odor Responses in a Detailed Olfactory Bulb Network Model responses in a detailed olfac- tory bulb network model. J Neurophysiol 90: 1921­1935, 2003. First published May 7, 2003; 10.1152/jn.00623.2002. In the olfactory bulb, both the spatial distribution

  12. Neuronal representation of odourants in the olfactory bulb of Xenopus laevis tadpoles

    E-Print Network [OSTI]

    Neuronal representation of odourants in the olfactory bulb of Xenopus laevis tadpoles Dirk Czesnik representation, olfactory bulb Abstract When an odourant enters the nose, olfactory receptor neurons (ORNs) convey information about it to the olfactory bulb (OB), where this information is processed and where

  13. Rhythm sequence through the olfactory bulb layers during the time window of a respiratory cycle

    E-Print Network [OSTI]

    Roux, Stephane

    Rhythm sequence through the olfactory bulb layers during the time window of a respiratory cycle 07, France Keywords: LFP, olfactory bulb, oscillations, rat, synchronization Abstract The mammalian olfactory bulb is characterized by prominent oscillatory activity of its local ®eld potentials. Breathing

  14. Structure of lateral inhibition in an olfactory bulb Andrew Davison, Jianfeng Feng, and David Brown

    E-Print Network [OSTI]

    Feng, Jianfeng

    Structure of lateral inhibition in an olfactory bulb model Andrew Davison, Jianfeng Feng, and David Abstract. It has been shown that mutual lateral inhibition of the projection neurones in the olfactory bulb, mediated by interneurones, serves to tune the representation of odours in the bulb and reduce the overlap

  15. How Many CASTLE Bulbs Would You Need To Match the Brightness of the Sun?

    E-Print Network [OSTI]

    Collar, Juan I.

    How Many CASTLE Bulbs Would You Need To Match the Brightness of the Sun? = × ? Please do all million miles), so if we were to try to build the Sun out of light bulbs, we'd need a bunch! But, how many objects: the Sun, and a CASTLE light bulb. What do we mean by "brightness?" The electromagnetic (EM

  16. THE EFFECT OF SNOWPACK WARMING ON THE STRESS BULB BELOW A SKIER Thomas Exner *

    E-Print Network [OSTI]

    Jamieson, Bruce

    THE EFFECT OF SNOWPACK WARMING ON THE STRESS BULB BELOW A SKIER Thomas Exner * Dept. of Geoscience exist to quantify or validate the temperature effect on the stress bulb below a skier. In this study we periods. Increasing temperatures of the near-surface layers altered the shape of the stress bulb, but so

  17. Labeling energy cost on light bulbs lowers implicit discount rates Jihoon Min a

    E-Print Network [OSTI]

    Michalek, Jeremy J.

    Analysis Labeling energy cost on light bulbs lowers implicit discount rates Jihoon Min a , Inês L considerably. To quantify the influence of factors that drive consumer choices for light bulbs, we conducted incandescent bulbs. About half of the total lighting service (in terms of lumens) was provided by incandes

  18. Linear programming applied to the ower sector: a Gladiolus bulb production case study

    E-Print Network [OSTI]

    Dragoti-Çela, Eranda

    Linear programming applied to the ¯ower sector: a Gladiolus bulb production case study Jose representative Brazilian bulb growing and trading company's decision making process, during the Gladiolus production planning activity. The pertinent LP model was focused on client's bulb requests to be attended

  19. THE LIGHT BULB PROBLEM 1 Ramamohan Paturi 2 Sanguthevar Rajasekaran 3 John Reif 3

    E-Print Network [OSTI]

    Reif, John H.

    THE LIGHT BULB PROBLEM 1 Ramamohan Paturi 2 Sanguthevar Rajasekaran 3 John Reif 3 Univ. of California, San Diego Univ. of Pennsylvania Duke University 1 #12; Running Title: The Light Bulb Problem, 1985 and 1988: We have a sequence of n random light bulbs each of which is either on or o# with equal

  20. Photoperiod Mediated Changes in Olfactory Bulb Neurogenesis and Olfactory Behavior in Male White-

    E-Print Network [OSTI]

    Nelson, Randy J.

    Photoperiod Mediated Changes in Olfactory Bulb Neurogenesis and Olfactory Behavior in Male White to long day lengths (LD) and short day lengths (SD) for 10 to 15 weeks and then examined olfactory bulb cell proliferation and survival using the thymidine analog BrdU, olfactory bulb granule cell morphology

  1. fMRI visualisation of transient activations in the rat olfactory bulb using short odour stimulations

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    fMRI visualisation of transient activations in the rat olfactory bulb using short odour in the olfactory bulb OB displays both spatial and temporal organization. The difficulty when assessing spatio of the olfactory bulb (OB). Hence, in this structure, the identity of odorants is associated with a unique spatial

  2. Calcium Influx through NMDA Receptors Directly Evokes GABA Release in Olfactory Bulb Granule Cells

    E-Print Network [OSTI]

    Strowbridge, Ben

    Calcium Influx through NMDA Receptors Directly Evokes GABA Release in Olfactory Bulb Granule Cells, Case Western Reserve University, Cleveland, Ohio 44106-4975 Recurrent inhibition in olfactory bulb on the activation of NMDA receptors. Using whole-cell recordings from rat olfactory bulb slices, we now show

  3. Free Energy Efficiency Kit includes CFL light bulbs,

    E-Print Network [OSTI]

    Rose, Annkatrin

    Free Energy Efficiency Kit Kit includes CFL light bulbs, spray foam, low-flow shower head, and more for discounted energy assessments. FREE HOME ENERGY EFFICIENCY SEMINAR N e w R i ver L i g ht & Pow e r a n d W! Building Science 101 Presentation BPI Certified Building Professionals will present home energy efficiency

  4. Influence du poids et de la densit de plantation des bulbes d'chalote sur les caractristiques de

    E-Print Network [OSTI]

    Boyer, Edmond

    Influence du poids et de la densité de plantation des bulbes d'échalote sur les caractéristiques de la récolte et la prolificité des bulbes-fils Joseph COHAT Corentin TROMEUR LN.R.A., Station damélioration de la Pomme de terre et des Plantes à bulbes, F 29207 Landerneau RÉSUMÉ Des bulbes d'échalote de

  5. Influence d'un trempage des bulbes dans une solution d'acide 2-chloro thylphosphonique (thphon) sur la

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    Influence d'un trempage des bulbes dans une solution d'acide 2-chloro éthylphosphonique (éthéphon la Pomme de terre et des Plantes à bulbes, F 29207 Landerneau RÉSUMÉ Le trempage des bulbes d augmentation du pourcentage de floraison. Ceci résulte d'une diminution du nombre de bulbes qui Ethéphon

  6. Quasars Are Not Light-Bulbs: Testing Models of Quasar Lifetimes with the Observed Eddington Ratio Distribution

    E-Print Network [OSTI]

    Hopkins, Philip F

    2008-01-01T23:59:59.000Z

    We use the observed distribution of Eddington ratios as a function of supermassive black hole (BH) mass to constrain models of AGN lifetimes and lightcurves. Given the observed AGN luminosity function, a model for AGN lifetimes (time above a given luminosity) translates directly to a predicted Eddington ratio distribution. Models for self-regulated BH growth, in which feedback produces a 'blowout' decay phase after some peak luminosity (shutting down accretion) make specific predictions for the lifetimes distinct from those expected if AGN are simply gas starved (without feedback) and very different from simple phenomenological 'light bulb' models. Present observations of the Eddington ratio distribution, spanning 5 decades in Eddington ratio, 3 in BH mass, and redshifts z=0-1, agree with the predictions of self-regulated models, and rule out 'light-bulb', pure exponential, and gas starvation models at high significance. We compare the Eddington ratio distributions at fixed BH mass and fixed luminosity (both ...

  7. Estimating Wet Bulb Globe Temperature Using Standard Meteorological Measurements

    SciTech Connect (OSTI)

    Hunter, C.H.

    1999-11-18T23:59:59.000Z

    The heat stress management program at the Department of Energy''s Savannah River Site (SRS) requires implementation of protective controls on outdoor work based on observed values of wet bulb globe temperature (WBGT). To ensure continued compliance with heat stress program requirements, a computer algorithm was developed which calculates an estimate of WBGT using standard meteorological measurements. In addition, scripts were developed to generate a calculation every 15 minutes and post the results to an Intranet web site.

  8. Magnetic fluorescent lamp having reduced ultraviolet self-absorption

    DOE Patents [OSTI]

    Berman, Samuel M. (San Francisco, CA); Richardson, Robert W. (Pelham, NY)

    1985-01-01T23:59:59.000Z

    The radiant emission of a mercury-argon discharge in a fluorescent lamp assembly (10) is enhanced by providing means (30) for establishing a magnetic field with lines of force along the path of electron flow through the bulb (12) of the lamp assembly, to provide Zeeman splitting of the ultraviolet spectral line. Optimum results are obtained when the magnetic field strength causes a Zeeman splitting of approximately 1.7 times the thermal line width.

  9. Development of Diagnostic Rules for a Dry Bulb Economizer Mixed Air Loop

    E-Print Network [OSTI]

    Underwood, D.

    1990-01-01T23:59:59.000Z

    knowledge base for a two fan variable air volume (VAV) air handling unit. Rules for the mixed air loop with a dry bulb economizer are presented....

  10. DOE Requires Westinghouse to Cease Sales of Two Light Bulb Models...

    Broader source: Energy.gov (indexed) [DOE]

    against Westinghouse Lighting Corporation, the company must cease sales of two light bulb models - medium based CFL basic model 15GLOBE652 (Westinghouse product code 3800400)...

  11. DOE Withdraws the Energy Star Label from 34 Compact Fluorescent Light Bulbs

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels DataDepartment of Energy Your Density Isn't Your Destiny: Theof"WaveInteractionsMaterials | DepartmentEnergy Will Not| Department

  12. Full Spectrum Light Therapy Full spectrum light bulbs are said to not only improve mood, but also

    E-Print Network [OSTI]

    Bates, Rebecca A.

    Full Spectrum Light Therapy Full spectrum light bulbs are said to not only improve mood, but also spectrum light bulbs produce light that is seen by the human eye in a bluish-white tint. Where is full

  13. Chapter 30: Quantum Physics 9. The tungsten filament in a standard light bulb can be considered a blackbody radiator.

    E-Print Network [OSTI]

    Kioussis, Nicholas

    . 1 Chapter 30: Quantum Physics 9. The tungsten filament in a standard light bulb can be considered frequency is that of infrared electromagnetic radiation, the light bulb radiates more energy in the infrared

  14. Olfactory deprivation increases dopamine D2 receptor density in the rat olfactory bulb

    SciTech Connect (OSTI)

    Guthrie, K.M.; Pullara, J.M.; Marshall, J.F.; Leon, M. (University of California, Irvine (USA))

    1991-05-01T23:59:59.000Z

    Unilateral olfactory deprivation during postnatal development results in significant anatomical and neurochemical changes in the deprived olfactory bulb. Perhaps the most dramatic neurochemical change is the loss of dopaminergic expression by neurons of the glomerular region. The authors describe here the effects of early olfactory deprivation on other elements of the bulb dopaminergic system, namely the dopamine receptors of the olfactory bulb. Rat pups had a single naris occluded on postnatal day 2 (PN2). On PN20 or PN60, animals were sacrificed and the bulbs were examined for catecholamine levels or D2 and D1 dopamine receptor binding. Receptor densities were quantified by in vitro autoradiography using the tritiated antagonists spiperone (D2) and SCH23390 (D1). Dopamine uptake sites were similarly examined using tritiated mazindol. No significant specific labeling of D1 or mazindol sites was observed in the olfactory bulbs of control or experimental animals at either age. Normal animals displayed prominent labeling of D2 sites in the glomerular and nerve layers. After 60 days of deprivation, deprived bulbs exhibited an average increase in D2 receptor density of 32%. As determined by Scatchard analysis, the mean values for Kd and Bmax were 0.134 nM and 293 fmol/mg protein in normal bulbs, and 0.136 nM and 403 fmol/mg protein in deprived bulbs. The results suggest that, as in the neostriatum, dopamine depletion in the olfactory bulb leads to an upregulation of D2 receptor sites. This change may represent an attempt by the system to adapt neurochemically to reduced dopaminergic activity and thereby maintain bulb function.

  15. Influence des conditions de conservation des bulbes d'chalote (Allium cepa L. var. aggregatum) sur leur

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    Influence des conditions de conservation des bulbes d'échalote (Allium cepa L. var. aggregatum) sur leur levée Joseph COHAT LN.R.A., Station d'Amélioration de la Pomme de terre & des Plantes à bulbes, F 29207 Landerneau RÉSUMÉ Les bulbes d'échalote de Jersey (type demi-longue) sont dormants au moment de la

  16. A Bit About Bulbs Night temperatures are cool, the air is almost crisp. The vegetable harvest is tapering

    E-Print Network [OSTI]

    New Hampshire, University of

    A Bit About Bulbs Night temperatures are cool, the air is almost crisp. The vegetable harvest! There is always something to do, and right now is the time to plant spring-flowering bulbs. Whether your preference is for naturalized or formal bulb plantings, on a small or large scale, keep kinds and colors

  17. Impairment of Neurogensis in the Olfactory Bulb of Transgenic Mice Overexpressing Human Wildtype Alpha Synuclein Under the Thy-1 Promoter

    E-Print Network [OSTI]

    Allman, John M.

    Impairment of Neurogensis in the Olfactory Bulb of Transgenic Mice Overexpressing Human Wildtype, cortex and olfactory bulb (Rockenstein et al., 2002). These mice demonstrate early and progressive motor by olfactory ensheathing glia, and eventually reach the olfactory bulb (Gritti et al., 2002). Recent studies

  18. Dynamical Mechanisms of Odor Processing in Olfactory Bulb Mitral Cells Daniel B. Rubin and Thomas A. Cleland

    E-Print Network [OSTI]

    Cleland, Thomas A.

    Dynamical Mechanisms of Odor Processing in Olfactory Bulb Mitral Cells Daniel B. Rubin and Thomas A- nisms of odor processing in olfactory bulb mitral cells. J Neuro- physiol 96: 555­568, 2006. First, conductance-based compartmental model of olfactory bulb mitral cells that exhibits the complex dynamical

  19. Ordered progression of nematogenesis from stem cells through differentiation stages in the tentacle bulb of Clytia hemisphaerica (Hydrozoa, Cnidaria)

    E-Print Network [OSTI]

    Gibson, Matt

    bulb of Clytia hemisphaerica (Hydrozoa, Cnidaria) Elsa Denker, Michaël Manuel, Lucas Leclère, Hervé Le of the tentacle bulb, a specialized swelling at the tentacle base. Analysis by a variety of light and electron-temporal characteristics are markedly more ordered. The tentacle bulb nematogenic ectoderm (TBE) was found to be polarized

  20. Stability of fast oscillations in mammalian olfactory bulb: experiment and modeling Nicolas Fourcaud-Trocmea,b,c,d,

    E-Print Network [OSTI]

    Voegtlin, Thomas

    Stability of fast oscillations in mammalian olfactory bulb: experiment and modeling Nicolas Cortex, Vandoeuvre-les-Nancy, F-54506, France Abstract In the rat olfactory bulb (OB), fast oscillations observed stability of the oscillatory regimes. Keywords: olfactory bulb, beta oscillations, gamma

  1. Odor vapor pressure and quality modulate local field potential oscillatory patterns in the olfactory bulb of the

    E-Print Network [OSTI]

    Roux, Stephane

    in the olfactory bulb of the anesthetized rat Tristan Cenier,1 Corine Amat,1 Philippe Litaudon,1 Samuel Garcia,1 potentials, molecular features, olfactory bulb, oscillations, respiratory cycle Abstract A central question-temporal pattern of activation in the olfactory bulb (OB), in both glomerular and mitral cell layers. Mitral

  2. Fluorescent refrigeration

    DOE Patents [OSTI]

    Epstein, Richard I. (Santa Fe, NM); Edwards, Bradley C. (Los Alamos, NM); Buchwald, Melvin I. (Santa Fe, NM); Gosnell, Timothy R. (Santa Fe, NM)

    1995-01-01T23:59:59.000Z

    Fluorescent refrigeration is based on selective radiative pumping, using substantially monochromatic radiation, of quantum excitations which are then endothermically redistributed to higher energies. Ultimately, the populated energy levels radiatively deexcite emitting, on the average, more radiant energy than was initially absorbed. The material utilized to accomplish the cooling must have dimensions such that the exciting radiation is strongly absorbed, but the fluorescence may exit the material through a significantly smaller optical pathlength. Optical fibers and mirrored glasses and crystals provide this requirement.

  3. Odor recognition and segmentation by coupled olfactory bulb and cortical networks

    E-Print Network [OSTI]

    Li, Z; Li, Zhaoping; Hertz, John

    1999-01-01T23:59:59.000Z

    We present a model of a coupled system of the olfactory bulb and cortex. Odor inputs to the epithelium are transformed to oscillatory bulbar activities. The cortex recognizes the odor by resonating to the bulbar oscillating pattern when the amplitude and phase patterns from the bulb match an odor memory stored in the intracortical synapses. We assume a cortical structure which transforms the odor information in the oscillatory pattern to a slow DC feedback signal to the bulb. This feedback suppresses the bulbar response to the pre-existing odor, allowing subsequent odor objects to be segmented out for recognition.

  4. LACKEY, PAUL E. An Investigation of Bridge Deck Overhang Falsework Systems Installed onto Bulb Tee Girders. (Under the direction of Dr. Emmett Sumner.)

    E-Print Network [OSTI]

    Bulb Tee Girders. (Under the direction of Dr. Emmett Sumner.) Bulb Tee girders provide a practical moments of inertia to withstand the massive moments created in the spans. A bulb tee girder possesses the thin top flange of an exterior bulb tee girder in the bridge deck overhang falsework system to fail

  5. Exam Stochastic Processes 2WB08 January 29, 2007 A light bulb burns for an amount of time having distribution F(), with Laplace trans-

    E-Print Network [OSTI]

    Giardinà, Cristian

    Exam Stochastic Processes 2WB08 ­ January 29, 2007 Problem 1: A light bulb burns for an amount moment µ2. When the light bulb burns out, it is immediately replaced by another light bulb which has the same life time distribution F(·), etc. Let m(t) be the mean number of replacements of light bulbs upto

  6. 600 New Lights Bulbs to Improve Energy Efficiency at DOE | Department...

    Broader source: Energy.gov (indexed) [DOE]

    our Washington, D.C., Forrestal North Building canopy with state of the art Light Emitting Diode (LED) fixtures. Every new bulb now uses just 23 watts instead of 205 watts....

  7. A critical period for activity-dependent synaptic development during olfactory bulb adult neurogenesis

    E-Print Network [OSTI]

    Kelsch, Wolfgang

    New neurons integrate in large numbers into the mature olfactory bulb circuit throughout life. The factors controlling the synaptic development of adult-born neurons and their connectivity remain essentially unknown. We ...

  8. Westinghouse Pays $50,000 Civil Penalty to Resolve Light Bulb...

    Broader source: Energy.gov (indexed) [DOE]

    here. Addthis Related Articles DOE Requires Westinghouse to Cease Sales of Two Light Bulb Models and Allows Sale of Another Air-Con Agrees to Pay Civil Penalty to Resolve...

  9. Fluorescent refrigeration

    DOE Patents [OSTI]

    Epstein, R.I.; Edwards, B.C.; Buchwald, M.I.; Gosnell, T.R.

    1995-09-05T23:59:59.000Z

    Fluorescent refrigeration is based on selective radiative pumping, using substantially monochromatic radiation, of quantum excitations which are then endothermically redistributed to higher energies. Ultimately, the populated energy levels radiatively deexcite emitting, on the average, more radiant energy than was initially absorbed. The material utilized to accomplish the cooling must have dimensions such that the exciting radiation is strongly absorbed, but the fluorescence may exit the material through a significantly smaller optical pathlength. Optical fibers and mirrored glasses and crystals provide this requirement. 6 figs.

  10. 2.44a0005 Physiology of the Main Olfactory Bulb Matthew Ennis1

    E-Print Network [OSTI]

    Hayar, Abdallah

    -inhibition 27 2.44.6.4.2 Lateral inhibition 27 2.44.6.4.3 Role of Ca2þ influx through NMDA receptors and voltage.44.8 Neurophysiology of Primary Olfactory Cortical Inputs to Main Olfactory Bulb 29 2.44.9 Oscillations and Synchrony in Main Olfactory Bulb 29 2.44.9.1 Oscillations 29 2.44.9.1.1 Theta rhythm 30 2.44.9.1.2 Gamma rhythm 30 2

  11. Interrelationships between air velocity and natural wet-bulb thermometer response

    E-Print Network [OSTI]

    Jones, Nathan Glenn

    1983-01-01T23:59:59.000Z

    INTERRELATIONSHIPS BETWEEN AIR VFLOCITY ANO NATURAL WET-BULB THERMOMETER RESPONSE A Thesis by NATHAN GLENN JONES Submitted to the Graduate Colleqe of Texas ASM University i n partial fulfillment of the requirement for the degree of MASTER... OF SCIENCE AUGUST 1983 Major Subject: Industrial Hygiene INTERRELATIONSHIPS BETWEEN AIR VELOCITY AND NATURAL WET-BULB THERMOMETER RESPONSE A Thesis by NATHAN GLENN JONES Approved as to style an content by: airman o ommittee er Member ~~' A~ Member...

  12. Fluorescence analyzer for lignin

    SciTech Connect (OSTI)

    Berthold, J.W.; Malito, M.L.; Jeffers, L.

    1993-06-01T23:59:59.000Z

    An apparatus for measuring lignin concentration with time resolved fluorescence in an undiluted wood pulp or black liquor sample, on a real-time, in situ basis is described, comprising: light source means for applying excitation light pulses at a selected wavelength and at known time intervals to the undiluted sample for causing the lignin concentration to produce fluorescent emission light with a fluorescence intensity that monotonically decreases in a quenched fluorescence regime; light detector means for measuring the emission light at the known time intervals and establishing signals indicative thereof; switching means for turning said light detector means on at precise specified time intervals after each excitation light pulse; and signal processing means connected to the light source means and the light detector means for comparing intensities of the emission light from the lignin in the quenched fluorescence regime to the intensities of the excitation light pulses on a time resolved basis for providing a measurement of the lignin concentration in the undiluted sample as a function of the time resolved emission light intensity.

  13. Quasars Are Not Light-Bulbs: Testing Models of Quasar Lifetimes with the Observed Eddington Ratio Distribution

    E-Print Network [OSTI]

    Philip F. Hopkins; Lars Hernquist

    2009-04-30T23:59:59.000Z

    We use the observed distribution of Eddington ratios as a function of supermassive black hole (BH) mass to constrain models of AGN lifetimes and lightcurves. Given the observed AGN luminosity function, a model for AGN lifetimes (time above a given luminosity) translates directly to a predicted Eddington ratio distribution. Models for self-regulated BH growth, in which feedback produces a 'blowout' decay phase after some peak luminosity (shutting down accretion) make specific predictions for the lifetimes distinct from those expected if AGN are simply gas starved (without feedback) and very different from simple phenomenological 'light bulb' models. Present observations of the Eddington ratio distribution, spanning 5 decades in Eddington ratio, 3 in BH mass, and redshifts z=0-1, agree with the predictions of self-regulated models, and rule out 'light-bulb', pure exponential, and gas starvation models at high significance. We compare the Eddington ratio distributions at fixed BH mass and fixed luminosity (both are consistent, but the latter are much less constraining). We present empirical fits to the lifetime distribution and show how the Eddington ratio distributions place tight limits on AGN lifetimes at various luminosities. We use this to constrain the shape of the typical AGN lightcurve, and provide simple analytic fits. Given independent constraints on episodic lifetimes, most local BHs must have gained their mass in no more than a couple of bright episodes, in agreement with merger-driven fueling models.

  14. Instrument uncertainty effect on calculation of absolute humidity using dewpoint, wet-bulb, and relative humidity sensors

    SciTech Connect (OSTI)

    Slayzak, S.J.; Ryan, J.P.

    1998-04-01T23:59:59.000Z

    As part of the US Department of Energy`s Advanced Desiccant Technology Program, the National Renewable Energy Laboratory (NREL) is characterizing the state-of-the-art in desiccant dehumidifiers, the key component of desiccant cooling systems. The experimental data will provide industry and end users with independent performance evaluation and help researchers assess the energy savings potential of the technology. Accurate determination of humidity ratio is critical to this work and an understanding of the capabilities of the available instrumentation is central to its proper application. This paper compares the minimum theoretical random error in humidity ratio calculation for three common measurement methods to give a sense of the relative maximum accuracy possible for each method assuming systematic errors can be made negligible. A series of experiments conducted also illustrate the capabilities of relative humidity sensors as compared to dewpoint sensors in measuring the grain depression of desiccant dehumidifiers. These tests support the results of the uncertainty analysis. At generally available instrument accuracies, uncertainty in calculated humidity ratio for dewpoint sensors is determined to be constant at approximately 2%. Wet-bulb sensors range between 2% and 6% above 10 g/kg (4%--15% below), and relative humidity sensors vary between 4% above 90% rh and 15% at 20% rh. Below 20% rh, uncertainty for rh sensors increases dramatically. Highest currently attainable accuracies bring dewpoint instruments down to 1% uncertainty, wet bulb to a range of 1%--3% above 10 g/kg (1.5%--8% below), and rh sensors between 1% and 5%.

  15. System and method for monitoring wet bulb temperature in a flue gas stream

    SciTech Connect (OSTI)

    Glover, R.L.; Bland, V.V.

    1990-01-02T23:59:59.000Z

    This patent describes in a system for monitoring wet bulb temperature in a flue gas stream means for extracting a sample of the gas from the flue, means for heating the sample to maintain the sample at substantially the same temperature as the gas in the flue, a sensor for measuring the wet bulb temperature of the sample, a reservoir of liquid, a liquid absorbent wick surrounding the sensor and extending into the liquid in the reservoir, and means for maintaining the liquid in the reservoir at a substantially constant level.

  16. Tired of changing light bulbs AND want to save money? Still using 100 year-old technology?

    E-Print Network [OSTI]

    Glashausser, Charles

    ?) ·Fine print: You will also reduce Global Warming pollution. Over its lifetime, a "100W" Compact will prevent global warming pollution equal to 100 to 150 gallons of gasoline AND reduce poisons like mercury bulb but lasts THIRTEEN times longer! The Compact bulb will save you $90 just in electricity cost over

  17. Bulbs for Winter Bloom The trees have all dropped their leaves and our gardens are covered by a blanket of snow. It is too

    E-Print Network [OSTI]

    New Hampshire, University of

    Bulbs for Winter Bloom The trees have all dropped their leaves and our gardens are covered into an inside garden. Paperwhite Narcissus and Amaryllis bulbs both produce beautiful flowers. They are easy bulbs to grow inside a house. Paperwhites come in two colors, white and yellow. They are extremely easy

  18. Instructions for Corning Model 220 pH Meter The electrode tip is a fragile glass bulb. Be careful or you will break it with a

    E-Print Network [OSTI]

    Cross, George

    Instructions for Corning Model 220 pH Meter The electrode tip is a fragile glass bulb. Be careful a polymer body electrode can create sufficient internal pressure to "explode" the glass bulb. When of cotton in the plastic electrode cover sleeve, add pH 7 buffer, and insert the electrode bulb

  19. UNH Cooperative Extension Info Line Question of the Week Q. What should I do with the Amaryllis bulb I just received?

    E-Print Network [OSTI]

    New Hampshire, University of

    bulb I just received? A. Few flowers give greater satisfaction than the spectacular amaryllis. Amaryllis are available from September to March as dormant bulbs ready for planting. They can be purchased as bulbs or as pre packaged units complete with pots and potting medium. Shortly after planting one or two

  20. Plant Disease Note 2004 | Iris Yellow Spot Virus in Onion Bulb and Seed Crops in Washington Overview Current Issue Past Issues Search PD Search APS Journals

    E-Print Network [OSTI]

    Pappu, Hanu R.

    Plant Disease Note 2004 | Iris Yellow Spot Virus in Onion Bulb and Seed Crops in Washington Yellow Spot Virus in Onion Bulb and Seed Crops in Washington. L. J. du Toit, Washington State University of the NP gene. In August 2003, symptoms of IYSV infection were observed in two onion bulb crops, each

  1. Contouring Variability of the Penile Bulb on CT Images: Quantitative Assessment Using a Generalized Concordance Index

    SciTech Connect (OSTI)

    Carillo, Viviana [Department of Medical Physics, San Raffaele Scientific Institute, Milano (Italy)] [Department of Medical Physics, San Raffaele Scientific Institute, Milano (Italy); Cozzarini, Cesare [Department of Radiotherapy, San Raffaele Scientific Institute, Milano (Italy)] [Department of Radiotherapy, San Raffaele Scientific Institute, Milano (Italy); Perna, Lucia; Calandra, Mauro [Department of Medical Physics, San Raffaele Scientific Institute, Milano (Italy)] [Department of Medical Physics, San Raffaele Scientific Institute, Milano (Italy); Gianolini, Stefano [Medical Software Solutions GmbH, Hagendorn (Switzerland)] [Medical Software Solutions GmbH, Hagendorn (Switzerland); Rancati, Tiziana [Prostate Cancer Program, IRCCS National Institute of Cancer, Milano (Italy)] [Prostate Cancer Program, IRCCS National Institute of Cancer, Milano (Italy); Spinelli, Antonello Enrico [Department of Medical Physics, San Raffaele Scientific Institute, Milano (Italy)] [Department of Medical Physics, San Raffaele Scientific Institute, Milano (Italy); Vavassori, Vittorio [Department of Radiotherapy, Cliniche Gavazzeni Humanitas, Bergamo (Italy)] [Department of Radiotherapy, Cliniche Gavazzeni Humanitas, Bergamo (Italy); Villa, Sergio [Department of Radiotherapy 1, IRCCS National Institute of Cancer, Milano (Italy)] [Department of Radiotherapy 1, IRCCS National Institute of Cancer, Milano (Italy); Valdagni, Riccardo [Prostate Cancer Program, IRCCS National Institute of Cancer, Milano (Italy) [Prostate Cancer Program, IRCCS National Institute of Cancer, Milano (Italy); Department of Radiotherapy 1, IRCCS National Institute of Cancer, Milano (Italy); Fiorino, Claudio, E-mail: fiorino.claudio@hsr.it [Department of Medical Physics, San Raffaele Scientific Institute, Milano (Italy)] [Department of Medical Physics, San Raffaele Scientific Institute, Milano (Italy)

    2012-11-01T23:59:59.000Z

    Purpose: Within a multicenter study (DUE-01) focused on the search of predictors of erectile dysfunction and urinary toxicity after radiotherapy for prostate cancer, a dummy run exercise on penile bulb (PB) contouring on computed tomography (CT) images was carried out. The aim of this study was to quantitatively assess interobserver contouring variability by the application of the generalized DICE index. Methods and Materials: Fifteen physicians from different Institutes drew the PB on CT images of 10 patients. The spread of DICE values was used to objectively select those observers who significantly disagreed with the others. The analyses were performed with a dedicated module in the VODCA software package. Results: DICE values were found to significantly change among observers and patients. The mean DICE value was 0.67, ranging between 0.43 and 0.80. The statistics of DICE coefficients identified 4 of 15 observers who systematically showed a value below the average (p value range, 0.013 - 0.059): Mean DICE values were 0.62 for the 4 'bad' observers compared to 0.69 of the 11 'good' observers. For all bad observers, the main cause of the disagreement was identified. Average DICE values were significantly worse from the average in 2 of 10 patients (0.60 vs. 0.70, p < 0.05) because of the limited visibility of the PB. Excluding the bad observers and the 'bad' patients,' the mean DICE value increased from 0.67 to 0.70; interobserver variability, expressed in terms of standard deviation of DICE spread, was also reduced. Conclusions: The obtained values of DICE around 0.7 shows an acceptable agreement, considered the small dimension of the PB. Additional strategies to improve this agreement are under consideration and include an additional tutorial of the so-called bad observers with a recontouring procedure, or the recontouring by a single observer of the PB for all patients included in the DUE-01 study.

  2. Volunteer Potato Density Influences Critical Time of Weed Removal in Bulb Onion

    E-Print Network [OSTI]

    Sims, Gerald K.

    Volunteer Potato Density Influences Critical Time of Weed Removal in Bulb Onion Martin M. Williams II, Corey V. Ransom, and W. Mack Thompson* Volunteer potato is highly competitive with onion and few control tactics are effective for removing this weed from an onion crop. Both volunteer potato density

  3. Some cultural practices affecting bulb rot, plant and floral development, and seed yield of the White Grano onion

    E-Print Network [OSTI]

    Enzie, Joseph Vincent

    1955-01-01T23:59:59.000Z

    L IBRARY A&IW COLLEGE OF TEXAS SOME CULTURAL PRACTICES AFFECTING BULB ROT, PLANT AND FLORAL DEVELOPMENT, AND SEED YIELD OF THE WHITE GRANO ONION A Dissertation By JOSEPH V. ENZIE Submitted to the Graduate School of the Agricultural... and Mechanical College of Texas in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY May 1955 Major Subject: Horticulture SOME CULTURAL PRACTICES AFFECTING BULB ROT, PLANT AND FLORAL DEVELOPMENT, AND SEED YIELD OF THE WHITE GRANO...

  4. Safe biodegradable fluorescent particles

    DOE Patents [OSTI]

    Martin, Sue I. (Berkeley, CA); Fergenson, David P. (Alamo, CA); Srivastava, Abneesh (Santa Clara, CA); Bogan, Michael J. (Dublin, CA); Riot, Vincent J. (Oakland, CA); Frank, Matthias (Oakland, CA)

    2010-08-24T23:59:59.000Z

    A human-safe fluorescence particle that can be used for fluorescence detection instruments or act as a safe simulant for mimicking the fluorescence properties of microorganisms. The particle comprises a non-biological carrier and natural fluorophores encapsulated in the non-biological carrier. By doping biodegradable-polymer drug delivery microspheres with natural or synthetic fluorophores, the desired fluorescence can be attained or biological organisms can be simulated without the associated risks and logistical difficulties of live microorganisms.

  5. Fish Passage Through a Simulated Horizontal Bulb Turbine Pressure Regime: A Supplement to"Laboratory Studies of the Effects of Pressure and Dissolved Gas Supersaturation on Turbine-Passed Fish"

    SciTech Connect (OSTI)

    Abernethy, Cary S. (BATTELLE (PACIFIC NW LAB)); Amidan, Brett G. (BATTELLE (PACIFIC NW LAB)); Cada, G F. (ORNL)

    2003-07-31T23:59:59.000Z

    Migratory and resident fish in the Columbia River Basin are exposed to stresses associated with hydroelectric power production, including pressure changes during turbine passage. The responses of fall chinook salmon and bluegill sunfish to rapid pressure change was investigated at the Pacific Northwest National Laboratory. Previous test series evaluated the effects of passage through a vertical Kaplan turbine under the"worst case" pressure conditions and under less severe conditions where pressure changes were minimized. For this series of tests, pressure changes were modified to simulate passage through a horizontal bulb turbine, commonly installed at low head dams. The results were compared to results from previous test series. Migratory and resident fish in the Columbia River Basin are exposed to stresses associated with hydroelectric power production, including pressure changes during turbine passage. The responses of fall chinook salmon and bluegill sunfish to rapid pressure change was investigated at the Pacific Northwest National Laboratory. Previous test series evaluated the effects of passage through a vertical Kaplan turbine under the"worst case" pressure conditions and under less severe conditions where pressure changes were minimized. For this series of tests, pressure changes were modified to simulate passage through a horizontal bulb turbine, commonly installed at low head dams. The results were compared to results from previous test series. Both fish species were acclimated for 16-22 hours at either surface (101 kPa; 1 atm) or 30 ft (191 kPa; 1.9 atm) of pressure in a hyperbaric chamber before exposure to a pressure scenario simulating passage through a horizontal bulb turbine. The simulation was as follows: gradual pressure increase to about 2 atm of pressure, followed by a sudden (0.4 second) decrease in pressure to either 0.7 or 0.95 atm, followed by gradual return to 1 atm (surface water pressure). Following the exposure, fish were held at surface pressure for a 48-hour post exposure observation period. No fall chinook salmon died during or after exposure to the horizontal bulb turbine passage pressures, and no injuries were observed during the 48-hour post exposure observation period. As with the previous test series, it cannot be determined whether fall chinook salmon acclimated to the greater water pressure during the pretest holding period. For bluegill sunfish exposed to the horizontal bulb turbine turbine-passage pressures, only one fish died and injuries were less severe and less common than for bluegills subjected to either the"worst case" pressure or modified Kaplan turbine pressure conditions in previous tests. Injury rates for bluegills were higher at 0.7 atm nadir than for the 0.95 atm nadir. However, injuries were limited to minor internal hemorrhaging. Bluegills did not suffer swim bladder rupture in any tested scenarios. Tests indicated that for most of the cross-sectional area of a horizontal bulb turbine, pressure changes occurring during turbine passage are not harmful to fall chinook salmon and only minimally harmful to bluegill. However, some areas within a horizontal bulb turbine may have extreme pressure conditions that would be harmful to fish. These scenarios were not tested because they represent a small cross-sectional area of the turbine compared to the centerline pressures scenarios used in these tests.

  6. Fluorescent Tube Lamps

    Broader source: Energy.gov [DOE]

    FEMP temporarily suspended its energy efficiency requirements for fluorescent tube lamps as it evaluates the market impact of the pending 2012 minimum efficiency standards for fluorescent lamps. The program will issue updated energy efficiency requirements when the market distribution of this product category stabilizes and when doing so has the potential to result in significant Federal energy savings.

  7. ONION (Allium cepa, 47 cultivars) B.K. Schroeder, Washington State University (WSU), Pullman, Enterobacter bulb decay; Enterobacter cloacae WA 99164; T.D. Waters, WSU Franklin Co. Extension, Pasco WA

    E-Print Network [OSTI]

    Schroeder, Brenda K.

    , Enterobacter bulb decay; Enterobacter cloacae WA 99164; T.D. Waters, WSU Franklin Co. Extension, Pasco WA 99301 in storage in Washington State, 2008-2009. An onion bulb storage trial was completed to survey 47 storage to Enterobacter cloacae, causal agent of Enterobacter bulb decay. Seeds of each cultivar were planted near Pasco

  8. ONION (Allium cepa, 55 cultivars) B.K. Schroeder, Washington State University (WSU), Pullman, Enterobacter bulb decay; Enterobacter cloacae WA 99164; T. Waters, WSU Franklin Co. Extension, Pasco WA

    E-Print Network [OSTI]

    Schroeder, Brenda K.

    , Enterobacter bulb decay; Enterobacter cloacae WA 99164; T. Waters, WSU Franklin Co. Extension, Pasco WA 99301 for resistance to Enterobacter cloacae in storage, 2007-2008. An onion bulb storage trial was completed to survey for resistance to Enterobacter cloacae, causal agent of Enterobacter bulb decay. Seeds of each cultivar were

  9. Recombinant fluorescent protein microsphere calibration standard

    DOE Patents [OSTI]

    Nolan, John P. (Santa Fe, NM); Nolan, Rhiannon L. (Santa Fe, NM); Ruscetti, Teresa (Los Alamos, NM); Lehnert, Bruce E. (Los Alamos, NM)

    2001-01-01T23:59:59.000Z

    A method for making recombinant fluorescent protein standard particles for calibration of fluorescence instruments.

  10. LED lamp or bulb with remote phosphor and diffuser configuration with enhanced scattering properties

    DOE Patents [OSTI]

    Tong, Tao; Le Toquin, Ronan; Keller, Bernd; Tarsa, Eric; Youmans, Mark; Lowes, Theodore; Medendorp, Jr., Nicholas W; Van De Ven, Antony; Negley, Gerald

    2014-11-11T23:59:59.000Z

    An LED lamp or bulb is disclosed that comprises a light source, a heat sink structure and an optical cavity. The optical cavity comprises a phosphor carrier having a conversions material and arranged over an opening to the cavity. The phosphor carrier comprises a thermally conductive transparent material and is thermally coupled to the heat sink structure. An LED based light source is mounted in the optical cavity remote to the phosphor carrier with light from the light source passing through the phosphor carrier. A diffuser dome is included that is mounted over the optical cavity, with light from the optical cavity passing through the diffuser dome. The properties of the diffuser, such as geometry, scattering properties of the scattering layer, surface roughness or smoothness, and spatial distribution of the scattering layer properties may be used to control various lamp properties such as color uniformity and light intensity distribution as a function of viewing angle.

  11. Fluorescent filtered electrophosphorescence

    DOE Patents [OSTI]

    Forrest, Stephen (Ann Arbor, MI); Sun, Yiru (Princeton, NJ); Giebink, Noel (Ann Arbor, MI); Thompson, Mark E. (Anaheim Hills, CA)

    2010-08-03T23:59:59.000Z

    The present invention relates to organic light emitting devices (OLEDs), and more specifically to OLEDS that emit light using a combination of fluorescent emitters and phosphorescent emitters for the efficient utilization of all of the electrically generated excitons.

  12. Fluorescent filtered electrophosphorescence

    DOE Patents [OSTI]

    Forrest, Stephen R. (Princeton, NJ); Sun, Yiru (Princeton, NJ); Giebink, Noel (Princeton, NJ); Thompson, Mark E. (Anaheim Hills, CA)

    2009-01-06T23:59:59.000Z

    The present invention relates to organic light emitting devices (OLEDs), and more specifically to OLEDS that emit light using a combination of fluorescent emitters and phosphorescent emitters for the efficient utilization of all of the electrically generated excitons.

  13. FAA Fluorescent Penetrant Laboratory Inspections

    SciTech Connect (OSTI)

    WINDES,CONNOR L.; MOORE,DAVID G.

    2000-08-02T23:59:59.000Z

    The Federal Aviation Administration Airworthiness Assurance NDI Validation Center currently assesses the capability of various non-destructive inspection (NDI) methods used for analyzing aircraft components. The focus of one such exercise is to evaluate the sensitivity of fluorescent liquid penetrant inspection. A baseline procedure using the water-washable fluorescent penetrant method defines a foundation for comparing the brightness of low cycle fatigue cracks in titanium test panels. The analysis of deviations in the baseline procedure will determine an acceptable range of operation for the steps in the inspection process. The data also gives insight into the depth of each crack and which step(s) of the inspection process most affect penetrant sensitivities. A set of six low cycle fatigue cracks produced in 6.35-mm thick Ti-6Al-4V specimens was used to conduct the experiments to produce sensitivity data. The results will document the consistency of the crack readings and compare previous experiments to find the best parameters for water-washable penetrant.

  14. Fluorescent image tracking velocimeter

    DOE Patents [OSTI]

    Shaffer, Franklin D. (Library, PA)

    1994-01-01T23:59:59.000Z

    A multiple-exposure fluorescent image tracking velocimeter (FITV) detects and measures the motion (trajectory, direction and velocity) of small particles close to light scattering surfaces. The small particles may follow the motion of a carrier medium such as a liquid, gas or multi-phase mixture, allowing the motion of the carrier medium to be observed, measured and recorded. The main components of the FITV include: (1) fluorescent particles; (2) a pulsed fluorescent excitation laser source; (3) an imaging camera; and (4) an image analyzer. FITV uses fluorescing particles excited by visible laser light to enhance particle image detectability near light scattering surfaces. The excitation laser light is filtered out before reaching the imaging camera allowing the fluoresced wavelengths emitted by the particles to be detected and recorded by the camera. FITV employs multiple exposures of a single camera image by pulsing the excitation laser light for producing a series of images of each particle along its trajectory. The time-lapsed image may be used to determine trajectory and velocity and the exposures may be coded to derive directional information.

  15. Ecological and molecular investigation of wheat bulb fly (Delia coarctata, Fallén, Diptera: Anthomyiidae) for the advancement of population monitoring and control methodologies 

    E-Print Network [OSTI]

    Rogers, Craig David

    2012-06-22T23:59:59.000Z

    Wheat bulb fly (WBF) (Delia coarctata, Fallén, Diptera: Anthomyiidae) is a pest of commercial importance in cereal crops. Control is dependent on organophosphates some of which are restricted in the UK, while current ...

  16. Fluorescence energy transfer enhancement in aluminum nanoapertures

    E-Print Network [OSTI]

    de Torres, Juan; Moparthi, Satish Babu; Grigoriev, Victor; Wenger, Jérome

    2015-01-01T23:59:59.000Z

    Zero-mode waveguides (ZMWs) are confining light into attoliter volumes, enabling single molecule fluorescence experiments at physiological micromolar concentrations. Among the fluorescence spectroscopy techniques that can be enhanced by ZMWs, F\\"{o}rster resonance energy transfer (FRET) is one of the most widely used in life sciences. Combining zero-mode waveguides with FRET provides new opportunities to investigate biochemical structures or follow interaction dynamics at micromolar concentration with single molecule resolution. However, prior to any quantitative FRET analysis on biological samples, it is crucial to establish first the influence of the ZMW on the FRET process. Here, we quantify the FRET rates and efficiencies between individual donor-acceptor fluorophore pairs diffusing in aluminum zero-mode waveguides. Aluminum ZMWs are important structures thanks to their commercial availability and the large literature describing their use for single molecule fluorescence spectroscopy. We also compare the ...

  17. Predictive factors for erectile dysfunction in men with prostate cancer after brachytherapy: Is dose to the penile bulb important?

    SciTech Connect (OSTI)

    Macdonald, A. Graham [Department of Radiation Oncology, Vancouver Cancer Centre, BC Cancer Agency, Vancouver, BC (Canada)]. E-mail: gmacdonald@easynet.co.uk; Keyes, Mira [Department of Radiation Oncology, Vancouver Cancer Centre, BC Cancer Agency, Vancouver, BC (Canada); Kruk, Alexandra [Department of Radiation Therapy, Vancouver Cancer Centre, BC Cancer Agency, Vancouver, BC (Canada); Duncan, Graeme [Department of Radiation Oncology, Vancouver Cancer Centre, BC Cancer Agency, Vancouver, BC (Canada); Moravan, Veronika [Population and Preventive Oncology, BC Cancer Agency, Vancouver, BC (Canada); Morris, W. James [Department of Radiation Oncology, Vancouver Cancer Centre, BC Cancer Agency, Vancouver, BC (Canada)

    2005-09-01T23:59:59.000Z

    Purpose: To determine predictive factors for postimplant erectile dysfunction (ED) in a cohort of patients, according to prospectively collected data; specifically, to assess the impact of penile bulb volume and D50 and D95 (dose covering 50% and 95% of the penile bulb volume, respectively) on ED. Methods and Materials: Three hundred forty-two patients were identified who were potent before implant and who had at least 2 years' follow-up. Patient, tumor, treatment, and dosimetric data were collected on all patients. Postimplant ED was defined according to both physician-documented and patient-documented outcome data. Binary logistic regression analysis was used to create multivariable models of predictors for ED at 1, 2, and 3 years after implant. Results: Physician-documented rates of ED were 57%, 48%, and 38% at 1, 2, and 3 years after implant, respectively. Patient-documented rates of ED were 70% and 66% at 1 and 2 years, respectively. Multivariable analyses revealed age and degree of preimplant erectile function to be consistently significant predictors of ED. Use of hormones was significant at the 1-year physician-documented ED endpoint but not thereafter, in keeping with the time course of testosterone recovery. Penile bulb volume, D50, and D95 were not found to be predictive for ED at any time point, in contrast to previous studies. In addition, planning ultrasound target volume, number of needles, and institutional case sequence number were significant predictors of ED at various time points, consistent with a traumatic etiology of ED. Conclusions: We found no evidence to support penile bulb dosimetry as an independent predictive factor for ED after implant, using physician-documented or patient-documented outcomes.

  18. Penile bulb dose and impotence after three-dimensional conformal radiotherapy for prostate cancer on RTOG 9406: Findings from a prospective, multi-institutional, phase I/II dose-escalation study

    SciTech Connect (OSTI)

    Roach, Mack [University of California San Francisco, San Francisco, CA (United States)]. E-mail: roach@radonc17.ucsf.edu; Winter, Kathryn [Radiation Therapy Oncology Group, Philadelphia, PA (United States); Michalski, Jeffrey M. [Washington University, St. Louis, MO (United States); Cox, James D. [3D Quality Assurance Center at Washington University St. Louis, MO (United States); Purdy, James A. [University of Texas M.D. Anderson Cancer Center, Houston, TX (United States); Bosch, Walter [University of Texas M.D. Anderson Cancer Center, Houston, TX (United States); Lin Xiao [University of Texas M.D. Anderson Cancer Center, Houston, TX (United States); Shipley, William S. [Massachusetts General Hospital, Boston MA (United States)

    2004-12-01T23:59:59.000Z

    Purpose: To assess the relationship between the dose to the bulb of the penis and the risk of impotence in men treated on Radiation Therapy Oncology Group (RTOG) 9406. Methods and materials: Men enrolled on a Phase I/II dose-escalation study, RTOG 9406, who were reported to be potent at entry and evaluable (n = 158) were selected for inclusion. Follow-up evaluations were scheduled every 3, 4, and 6 months for the first, second, and the third through fifth years, then annually. At each follow-up visit an assessment of potency status was made. Penile structures were defined by a single observer blinded to the potency status, using Web-based, on-line software. The dosimetry for penile structures was calculated at the Quality Assurance Center at Washington University and provided to RTOG Statistical Headquarters to determine whether there was a relationship between dose and impotence. Results: Patients whose median penile dose was {>=}52.5 Gy had a greater risk of impotence compared with those receiving <52.5 Gy (p = 0.039). In a multivariate analysis neither age, the dose to the prostate, nor the use of hormonal therapy correlated with the risk of impotence. Conclusions: Dose to the bulb of the penis seems to be associated with the risk of radiation-induced impotence.

  19. EPI-FLUORESCENCE ATTACHMENT f o r I n v e r t e d M i c r o s c o p e D I A P H O T -T M D

    E-Print Network [OSTI]

    Kleinfeld, David

    . In exchanging the lamp bulb, do not touch the glass part of the lamp bulb with bare hands. Dust, dirt or finger marks on the bulb surface should be wiped with alcohol. For attaching the mercury lamp bulb to the socket, take note of the polarity of the bulb. Before lighting the lamp, confirm that the lamp bulb

  20. Multiphoton Add fluorescent dye

    E-Print Network [OSTI]

    Braun, Paul

    in toluene was prepared at 0.96 w/w. The monomer used was trimethylolpropane triacrylate (TMPTA) (Aldrich in toluene; the toluene was removed by rotovapping. For fluorescence imaging in colloidal crystals the electro-optic modulator is used to regulate the power of the beam, selectively exposing the regions

  1. Optical Shelving: Suppressed Fluorescence

    E-Print Network [OSTI]

    Mould, R A

    2007-01-01T23:59:59.000Z

    The shelving phenomenon of quantum optics, originally observed by Dehmelt, is analyzed in terms of the nRules that are given in another paper. The heuristic value of these rules is apparent because they reveal the mechanism that enforces the suppression of fluorescence during the dark period associated with shelving.

  2. Optical Shelving: Suppressed Fluorescence

    E-Print Network [OSTI]

    Richard A Mould

    2011-04-11T23:59:59.000Z

    The shelving phenomenon of quantum optics, originally observed by Dehmelt, is analyzed in terms of the qRules that are given in another paper. The heuristic value of these rules is apparent because they not only describe the dark period during shelving, but they reveal the mechanism that enforces the suppression of fluorescence during that time.

  3. Fluorescent Protein Applications in Microscopy

    E-Print Network [OSTI]

    Straight, Aaron

    . The Identification of Green Fluorescent Protein III. Formation of the GFP Chromophore IV. The Structure of GFP V environment. II. The Identification of Green Fluorescent Protein The isolation of green fluorescent protein of Aequorea, Shimomura et al. noted that the lumines- cence from aequorin was blue rather than the green

  4. Renewable Surface Fluorescence Sandwich Immunoassay Biosensor...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Renewable Surface Fluorescence Sandwich Immunoassay Biosensor for Rapid Sensitive Botulinum Toxin Detection in an Automated Renewable Surface Fluorescence Sandwich Immunoassay...

  5. Single-molecule Fluorescence Spectroelectrochemistry of Cresyl...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Fluorescence Spectroelectrochemistry of Cresyl Violet. Abstract: We coupled scanning fluorescence microscopy with a potentiostat via a three-electrode electrochemical...

  6. The Air-Fluorescence Yield

    E-Print Network [OSTI]

    F. Arqueros; F. Blanco; D. Garcia-Pinto; M. Ortiz; J. Rosado

    2008-07-30T23:59:59.000Z

    Detection of the air-fluorescence radiation induced by the charged particles of extensive air showers is a well-established technique for the study of ultra-high energy cosmic rays. Fluorescence telescopes provide a nearly calorimetric measure of the primary energy. Presently the main source of systematic uncertainties comes from our limited accuracy in the fluorescence yield, that is, the number of fluorescence photons emitted per unit of energy deposited in the atmosphere by the shower particles. In this paper the current status of our knowledge on the fluorescence yield both experimental an theoretical will be discussed.

  7. STORM/PALM - Super Resolution Fluorescence Microscope | EMSL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    STORMPALM - Super Resolution Fluorescence Microscope STORMPALM - Super Resolution Fluorescence Microscope EMSL has developed and offers Fluorescence, Super Resolution STORM...

  8. Fluorescence analyzer for lignin

    SciTech Connect (OSTI)

    Berthold, John W. (Salem, OH); Malito, Michael L. (Hubbard, OH); Jeffers, Larry (Alliance, OH)

    1993-01-01T23:59:59.000Z

    A method and apparatus for measuring lignin concentration in a sample of wood pulp or black liquor comprises a light emitting arrangement for emitting an excitation light through optical fiber bundles into a probe which has an undiluted sensing end facing the sample. The excitation light causes the lignin concentration to produce fluorescent emission light which is then conveyed through the probe to analyzing equipment which measures the intensity of the emission light. Measures a This invention was made with Government support under Contract Number DOE: DE-FC05-90CE40905 awarded by the Department of Energy (DOE). The Government has certain rights in this invention.

  9. Fluorescent temperature sensor

    DOE Patents [OSTI]

    Baker, Gary A [Los Alamos, NM; Baker, Sheila N [Los Alamos, NM; McCleskey, T Mark [Los Alamos, NM

    2009-03-03T23:59:59.000Z

    The present invention is a fluorescent temperature sensor or optical thermometer. The sensor includes a solution of 1,3-bis(1-pyrenyl)propane within a 1-butyl-1-1-methylpyrrolidinium bis(trifluoromethylsulfonyl)imide ionic liquid solvent. The 1,3-bis(1-pyrenyl)propane remains unassociated when in the ground state while in solution. When subjected to UV light, an excited state is produced that exists in equilibrium with an excimer. The position of the equilibrium between the two excited states is temperature dependent.

  10. Identification of Nucleotides with Identical Fluorescent Labels Based on Fluorescence

    E-Print Network [OSTI]

    Myrick, Michael Lenn

    Identification of Nucleotides with Identical Fluorescent Labels Based on Fluorescence Polarization for discriminating among the four DNA nucleotides la- beled identically with tetramethylrhodamine is described and demonstrated. Labeled nucleotides were dissolved in buffered surfactant solutions. In room temperature 4.5 m

  11. Nuclear Resonance Fluorescence for Materials Assay

    E-Print Network [OSTI]

    Quiter, Brian

    2010-01-01T23:59:59.000Z

    clandestine material with nuclear resonance fluorescence”.E. Norman, UC Berkeley Dept. of Nuclear Engineering, privatepp. 349. G. Warren et al. “Nuclear Resonance Fluorescence of

  12. Determination of the 1%, 2.5%, and 5% occurrences of extreme dew-point temperatures and mean coincident dry-bulb temperatures

    SciTech Connect (OSTI)

    Colliver, D.G.; Zhang, H.; Gates, R.; Priddy, T. [Univ. of Kentucky, Lexington, KY (United States)

    1995-12-31T23:59:59.000Z

    The purpose of ASHRAE Research Project RP-754 was to find the outdoor design occurrences of extreme dew-point temperature and the corresponding mean coincident dry-bulb temperature for a large number of locations in North America. Thirty years of data (1961--1990) were used for 239 US locations, and the last 30 years of hourly data available (typically 1960--1989) were used for 143 Canadian locations. Tables are given that present the 1%, 2.5%, and 5% extreme summertime occurrences of dew-point temperatures and corresponding values of humidity ratios and the mean coincident dry-bulb (MCDB) temperature for the period of record. A map that shows the general geographical trends for the spatial distribution of the design dew-point values is also presented. Comparisons are provided between interpolation techniques to fill missing data, different weather data sets, and methods of calculating different definitions of extreme design and mean coincident data. Results are also reported of a comparison to evaluate the difference in humidity ratios between that calculated from the extreme design dry-bulb temperature and mean coincident wet-bulb temperature, versus that calculated from the extreme design dew-point temperature and the MCDB. It was found that the method of extreme dew point with MCDB had a higher humidity ratio that was on the average (for the 1% case and the 239 US locations) 0.00564 lb/lb larger than the humidity ratio found with the other method.

  13. Fluorescent fluid interface position sensor

    DOE Patents [OSTI]

    Weiss, Jonathan D.

    2004-02-17T23:59:59.000Z

    A new fluid interface position sensor has been developed, which is capable of optically determining the location of an interface between an upper fluid and a lower fluid, the upper fluid having a larger refractive index than a lower fluid. The sensor functions by measurement, of fluorescence excited by an optical pump beam which is confined within a fluorescent waveguide where that waveguide is in optical contact with the lower fluid, but escapes from the fluorescent waveguide where that waveguide is in optical contact with the upper fluid.

  14. Breaking the symmetries of the bulb model in two-dimensional self-induced supernova neutrino flavor conversions

    E-Print Network [OSTI]

    Mirizzi, Alessandro

    2015-01-01T23:59:59.000Z

    Self-induced flavor conversions of supernova (SN) neutrinos have been characterized in the spherically symmetric "bulb model", reducing the neutrino evolution to a one dimensional problem along a radial direction. We lift this assumption, presenting a two-dimensional model where neutrinos are launched from a spherical neutrino-sphere with many zenithal angles and two azimuthal angles. We also assume that self-induced conversions are not suppressed by large matter effects. In this situation we find that self-interacting neutrinos spontaneously break axial and spherical symmetries. As a result the flavor content and the lepton number of the neutrino gas would acquire seizable direction-dependent variations, breaking the coherent behavior found in the spherically symmetric case. This finding would suggest that the previous results of the self-induced flavor evolution obtained in one-dimensional models should be critically re-examined.

  15. Nuclear Resonance Fluorescence for Nuclear Materials Assay

    E-Print Network [OSTI]

    Quiter, Brian Joseph

    2010-01-01T23:59:59.000Z

    Potential of Nuclear Resonance Fluorescence . . . . . . . .2.9.1 Nuclear ThomsonSections . . . . . . . . . . . . . . . Nuclear Resonance

  16. GREEN FLUORESCENT PROTEIN The green revolution

    E-Print Network [OSTI]

    Stearns, Tim

    GREEN FLUORESCENT PROTEIN The green revolution Green fluorescent protein allows gene expression a fluorescent product when expressed. Just such a molecule, green fluorescent protein (GFP), has recently green light when disturbed (often seen when riding in a boat at night). In Aequorea, the green

  17. Fluorescence spectrum analysis using Fourier series modeling for Fluorescein solution in Ethanol

    E-Print Network [OSTI]

    Hadi, Mahasin F

    2011-01-01T23:59:59.000Z

    We have measured the fluorescence spectrum for fluorescein solution in ethanol with concentration 1 {\\times} 10-3 mol/liter at different temperatures from room temperature to freezing point of solvent, (T = 153, 183, 223, 253, and 303 K) using liquid nitrogen. Table curve 2D version 5.01 program has been used to determine the fitting curve and fitting equation for each fluorescence spectrum. Fourier series (3 {\\times} 2) was the most suitable fitting equation for all spectra. Theoretical fluorescence spectrum of fluorescein in ethanol at T = 183K was calculated and compared with experimental fluorescence spectrum at the same temperature. There is a good similarity between them.

  18. Fluorescence of molecular excimers

    SciTech Connect (OSTI)

    Nurmukhametov, R.N.; Sakhno, T.V.; Saukov, G.G.; Khakhel, O.A. [L. Ya. Karpov Research Physiochemical Institute, Moscow (Russian Federation)

    1995-11-01T23:59:59.000Z

    The phenomena of excimer fluorescence are most thoroughly studied in solutions of polycyclic aromatic hydrocarbons (PAHs). Therefore, excimer formation is usually treated with reference to molecules of this class of compounds. In the literature, only a qualitative picture of the energetics of excimer formation is given. It is assumed that dimerization of electron-excited and unexcited molecules is followed by the splitting of molecular electron-excited levels. PAH molecules are characterized by two lower excited levels: {sup 1}L{sub a} and {sup 1}L{sub b} (according to Platt`s classification). The S{sub 1}* state of some PAH compounds (e.g., naphthalene, phenanthrene, pyrene) is {sup 1}L{sub b}, while in other PAH (anthracene, naphthacene, perylene, etc.) it is the {sup 1}L{sub a} state. It is assumed that the {sup 1}L{sub a}-level is split more significantly than the {sup 1}L{sub b} level. Therefore, for all PAH investigated the excimer state is described as a lower-lying component of the split {sup 1}L{sub a} level. Quantum-chemical consideration of the splitting of electron levels of PAH molecules in excimers is undertaken. Unfortunately, in this case the description is also of a qualitative character. In the cited work, a correlation is noted between the energy of the {sup 1}L{sub a} state of the molecule and the wave number corresponding to the maximum of the emission band of the excimer. However, it does not give the wavelength of the maximum of the excimer band for some PAH, in other words, a definite dependence of the position of this band on molecular structure. The present work is devoted to a search for an answer to this question.

  19. Engineering and Characterization of a Superfolder Green Fluorescent Protein

    SciTech Connect (OSTI)

    Pedelacq,J.; Cabantous, S.; Tran, T.; Terwilliger, T.; Waldo, G.

    2006-01-01T23:59:59.000Z

    Existing variants of green fluorescent protein (GFP) often misfold when expressed as fusions with other proteins. We have generated a robustly folded version of GFP, called 'superfolder' GFP, that folds well even when fused to poorly folded polypeptides. Compared to 'folding reporter' GFP, a folding-enhanced GFP containing the 'cycle-3' mutations and the 'enhanced GFP' mutations F64L and S65T, superfolder GFP shows improved tolerance of circular permutation, greater resistance to chemical denaturants and improved folding kinetics. The fluorescence of Escherichia coli cells expressing each of eighteen proteins from Pyrobaculum aerophilum as fusions with superfolder GFP was proportional to total protein expression. In contrast, fluorescence of folding reporter GFP fusion proteins was strongly correlated with the productive folding yield of the passenger protein. X-ray crystallographic structural analyses helped explain the enhanced folding of superfolder GFP relative to folding reporter GFP.

  20. Covered Product Category: Fluorescent Ballasts

    Broader source: Energy.gov [DOE]

    FEMP provides acquisition guidance and federal efficiency requirements across a variety of product categories, including fluorescent ballasts, which is a FEMP designated product category. Federal laws and requirements mandate that agencies meet these efficiency requirements in all procurement and acquisition actions that are not specifically exempted by law.

  1. Covered Product Category: Fluorescent Luminaires

    Broader source: Energy.gov [DOE]

    FEMP provides acquisition guidance and Federal efficiency requirements across a variety of product categories, including fluorescent luminaires. Federal laws and requirements mandate that agencies meet these efficiency requirements in all procurement and acquisition actions that are not specifically exempted by law.

  2. There Is No Correlation Between Erectile Dysfunction and Dose to Penile Bulb and Neurovascular Bundles Following Real-Time Low-Dose-Rate Prostate Brachytherapy

    SciTech Connect (OSTI)

    Solan, Amy N. [Department of Radiation Oncology, Mount Sinai School of Medicine, New York, NY (United States)], E-mail: amy.solan@mountsinai.org; Cesaretti, Jamie A. [Department of Radiation Oncology, Mount Sinai School of Medicine, New York, NY (United States); Stone, Nelson N. [Department of Urology, Mount Sinai School of Medicine, New York, NY (United States); Stock, Richard G. [Department of Radiation Oncology, Mount Sinai School of Medicine, New York, NY (United States)

    2009-04-01T23:59:59.000Z

    Purpose: We evaluated the relationship between the onset of erectile dysfunction and dose to the penile bulb and neurovascular bundles (NVBs) after real-time ultrasound-guided prostate brachytherapy. Methods and Materials: One hundred forty-seven patients who underwent prostate brachytherapy met the following eligibility criteria: (1) treatment with {sup 125}I brachytherapy to a prescribed dose of 160 Gy with or without hormones without supplemental external beam radiation therapy, (2) identification as potent before the time of implantation based on a score of 2 or higher on the physician-assigned Mount Sinai Erectile Function Score and a score of 16 or higher on the abbreviated International Index of Erectile Function patient assessment, and (3) minimum follow-up of 12 months. Median follow-up was 25.7 months (range, 12-47 months). Results: The 3-year actuarial rate of impotence was 23% (34 of 147 patients). An additional 43% of potent patients (49 of 113 patients) were using a potency aid at last follow-up. The penile bulb volume receiving 100% of the prescription dose (V{sub 100}) ranged from 0-0.05 cc (median, 0 cc), with a dose to the hottest 5% (D{sub 5}) range of 12.5-97.9 Gy (median, 40.8 Gy). There was no correlation between penile bulb D{sub 5} or V{sub 100} and postimplantation impotency on actuarial analysis. For the combined right and left NVB structures, V{sub 100} range was 0.3-5.1 cc (median, 1.8 cc), and V{sub 150} range was 0-1.5 cc (median, 0.31 cc). There was no association between NVB V{sub 100} or V{sub 150} and postimplantation impotency on actuarial analysis. Conclusion: Penile bulb doses are low after real-time ultrasound-guided prostate brachytherapy. We found no correlation between dose to either the penile bulb or NVBs and the development of postimplantation impotency.

  3. Fluorescent Magnetic Nanoparticles for Biomedical Applications

    E-Print Network [OSTI]

    Candea, George

    Fluorescent Magnetic Nanoparticles for Biomedical Applications V.M.Dao, Dr. G. Coullerez, Dr. L, the main goal was to synthesize and to characterize novel fluorescent magnetic nanoparticles. These nanoparticles (NPs) involve superparamagnetic iron oxide nanoparticles (SPIONs), a fluorescently-labeled polymer

  4. A cryogenic fluorescence spectroscopic study of uranyl carbonate...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    fluorescence spectroscopic study of uranyl carbonate, phosphate, and oxyhydroxide minerals. A cryogenic fluorescence spectroscopic study of uranyl carbonate, phosphate, and...

  5. Comparative Fellowships

    E-Print Network [OSTI]

    Watkins, Joseph C.

    Comparative Genomics IGERT Graduate Fellowships & Training Integrative Graduate Education for genomics, including genotyping, sequencing, microarray research, and computers for biocomputing needs. · Genomic Analysis and Technology Core · Biotechnology Computing Facility · Protemics Analysis Lab · Bio5

  6. Test Comparability

    E-Print Network [OSTI]

    Keller, Christine; Shulenburger, David E.

    2010-01-01T23:59:59.000Z

    KU ScholarWorks | http://kuscholarworks.ku.edu Test Comparability 2010 by Christine Keller and David Shulenburger This work has been made available by the University of Kansas Libraries’ Office of Scholarly Communication and Copyright. Please... and Shulenburger, David. “Test comparability,” with Christine Keller in the Letters section of Change, September/October 2010, p. 6. Published version: http://www.changemag.org/Archives/Back%20 Issues/September-October%202010/letters-to-editor.html Terms of Use...

  7. Fluorescence based chemical sensors for corrosion detection

    SciTech Connect (OSTI)

    Johnson, R.E. [LeTourneau Univ., Longview, TX (United States). Dept. of Chemistry; Agarwala, V.S. [Naval Air Warfare Center Aircraft Div., Patuxent River, MD (United States)

    1997-12-01T23:59:59.000Z

    Several fluorescent materials have been identified as possible corrosion sensing coatings. These are either redox or metal ion complex materials. The redox materials are nonfluorescent in the reduced state and become fluorescent upon oxidation. Incorporated into paint coatings, they provide an early warning of corrosive conditions at the metal or alloy surface. The metal ion complex materials only fluoresce when the organic compound complexes with metal ions such as those generated in corrosion reactions. Fluorescent materials have been incorporated into paint coatings and on metal surfaces for the detection of corrosion. Oxine reacts with aluminum oxide on corroded aluminum to give a fluorescence that can be photographed in UV light. Several other materials were found to have good fluorescence but cannot be reversibly oxidized or reduced at the present time. More work will be done with these compounds as well as with Schiff bases to develop new fluorescent chemical sensing materials for smart coating on alloy surfaces.

  8. Fluorescent optical liquid level sensor

    DOE Patents [OSTI]

    Weiss, Jonathan D. (Albuquerque, NM)

    2001-01-01T23:59:59.000Z

    A liquid level sensor comprising a transparent waveguide containing fluorescent material that is excited by light of a first wavelength and emits at a second, longer wavelength. The upper end of the waveguide is connected to a light source at the first wavelength through a beveled portion of the waveguide such that the input light is totally internally reflected within the waveguide above an air/liquid interface in a tank but is transmitted into the liquid below this interface. Light is emitted from the fluorescent material only in those portions of the waveguide that are above the air/liquid interface, to be collected at the upper end of the waveguide by a detector that is sensitive only to the second wavelength. As the interface moves down in the tank, the signal strength from the detector will increase.

  9. Comparison of Air Fluorescence and Ionization Measurements of E.M. Shower Depth Profiles: Test of a UHECR Detector Technique

    SciTech Connect (OSTI)

    Belz, J.; Cao, Z.; Huentemeyer, P.; Jui, C.C.H.; Martens, K.; Matthews, J.; Maestas, M.; Smith, J.; Sokolsky, P.; Springer, R.W.; Thomas, J.; Thomas, S.; /Utah U.; Chen,P.; Field, Clive; Hast, C.; Iverson, R.; Ng, J.S.T.; Odian, A.; Reil, K.; Vincke, H.; Walz, D.; /SLAC /Montana U. /Rutgers U., Piscataway /Taiwan, Natl. Taiwan U.; ,

    2005-10-07T23:59:59.000Z

    Measurements are reported on the fluorescence of air as a function of depth in electromagnetic showers initiated by bunches of 28.5 GeV electrons. The light yield is compared with the expected and observed depth profiles of ionization in the showers. It validates the use of atmospheric fluorescence profiles in measuring ultra high energy cosmic rays.

  10. Improved model for the analysis of air fluorescence induced by electrons

    E-Print Network [OSTI]

    F. Arqueros; F. Blanco; J. Rosado

    2008-07-24T23:59:59.000Z

    A model recently proposed for the calculation of air-fluorescence yield excited by electrons is revisited. Improved energy distributions of secondary electrons and a more realistic Monte Carlo simulation including some additional processes have allowed us to obtain more accurate results. The model is used to study in detail the relationship between fluorescence intensity and deposited energy in a wide range of primary energy (keVs - GeVs). In addition, predictions on the absolute value of the fluorescence efficiency in the absence of collisional quenching will be presented and compared with available experimental data.

  11. Fluorescent Nanoparticles for Radiation DetectionFluorescent Nanoparticles

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOEThe Bonneville Power AdministrationField8,Dist.New MexicoFinancingProofWorking OutsideFluorescent Lightingfor

  12. Saccharide sensing molecules having enhanced fluorescent properties

    DOE Patents [OSTI]

    Satcher Jr., Joe H.; Lane, Stephen M.; Darrow, Christopher B.; Cary, Douglas R.; Tran, Joe Anh

    2004-01-06T23:59:59.000Z

    The present invention provides formulae for fluorescent compounds that have a number of properties which make them uniquely suited for use in sensors of analytes such as saccharides. The advantageous fluorescent properties include favorable excitation wavelengths, emission wavelengths, fluorescence lifetimes, and photostability. Additional advantageous properties include enhanced aqueous solubility, as well as temperature and pH sensitivity. The compound comprises an aryl or a substituted phenyl botonic acid that acts as a substrate recognition component, a fluorescence switch component, and a fluorophore. Fluorescent compounds are described that are excited at wavelengths greater than 400 nm and emit at wavelengths greater than 450 nm, which is advantageous for optical transmission through skin. The fluorophore is typically selected from transition metal-ligand complexes and thiazine, oxazine, oxazone, or oxazine-one as well as anthracene compounds. The fluorescent compound can be immobilized in a glucose permeable biocompatible polymer matrix that is implantable below the skin.

  13. Scanning fluorescent microthermal imaging apparatus and method

    DOE Patents [OSTI]

    Barton, Daniel L. (Albuquerque, NM); Tangyunyong, Paiboon (Albuquerque, NM)

    1998-01-01T23:59:59.000Z

    A scanning fluorescent microthermal imaging (FMI) apparatus and method is disclosed, useful for integrated circuit (IC) failure analysis, that uses a scanned and focused beam from a laser to excite a thin fluorescent film disposed over the surface of the IC. By collecting fluorescent radiation from the film, and performing point-by-point data collection with a single-point photodetector, a thermal map of the IC is formed to measure any localized heating associated with defects in the IC.

  14. Scanning fluorescent microthermal imaging apparatus and method

    DOE Patents [OSTI]

    Barton, D.L.; Tangyunyong, P.

    1998-01-06T23:59:59.000Z

    A scanning fluorescent microthermal imaging (FMI) apparatus and method is disclosed, useful for integrated circuit (IC) failure analysis, that uses a scanned and focused beam from a laser to excite a thin fluorescent film disposed over the surface of the IC. By collecting fluorescent radiation from the film, and performing point-by-point data collection with a single-point photodetector, a thermal map of the IC is formed to measure any localized heating associated with defects in the IC. 1 fig.

  15. Laser-Stimulated Fluorescence in Paleontology

    E-Print Network [OSTI]

    Kaye, Thomas G.; Falk, Amanda Renee; Pittman, Michael; Sereno, Paul C.; Martin, Larry D.; Burnham, David A.; Gong, Enpu; Xu, Xing; Wang, Yinan

    2015-05-27T23:59:59.000Z

    . doi: 10.1371/journal.pone. 0009223 PMID: 20169153 8. Warren TS, Gleason S, Bostwick RC, Verbeek ER. Ultraviolet light and fluorescent minerals: under- standing, collecting and displaying fluorescent minerals. GemGuides Book. 1999. 9. Hibbs AR. Confocal... fake and genuine vertebrate fossils. Journal of Paleontological Techniques. 2008; 2:1–5. Laser-Stimulated Fluorescence in Paleontology PLOS ONE | DOI:10.1371/journal.pone.0125923 May 27, 2015 22 / 22 ...

  16. LED Replacements for Linear Fluorescent Lamps Webcast

    Broader source: Energy.gov [DOE]

    In this June 20, 2011 webcast on LED products marketed as replacements for linear fluorescent lamps, Jason Tuenge of the Pacific Northwest National Laboratory (PNNL) discussed current Lighting...

  17. Portable spotter for fluorescent contaminants on surfaces

    DOE Patents [OSTI]

    Schuresko, Daniel D. (Oak Ridge, TN)

    1980-01-01T23:59:59.000Z

    A portable fluorescence-based spotter for polynuclear aromatic hydrocarbon contamination on personnel and work area surfaces under ambient lighting conditions is provided. This instrument employs beam modulation and phase sensitive detection for discriminating between fluorescence from organic materials from reflected background light and inorganic fluorescent material. The device uses excitation and emission filters to provide differentiation between classes of aromatic organic compounds. Certain inorganic fluorescent materials, including heavy metal compounds, may also be distinguished from the organic compounds, despite both having similar optical properties.

  18. Covered Product Category: Compact Fluorescent Lamps

    Broader source: Energy.gov [DOE]

    FEMP provides acquisition guidance across a variety of product categories, including compact fluorescent lamps (CFLs), which are an ENERGY STAR-qualified product category.

  19. Energy Efficiency and Conservation Block Grant Program

    Broader source: Energy.gov (indexed) [DOE]

    approximately 277 conventional60-watt light bulbs in tribal homes with 13-watt Light Emitting Diode (LED) bulbs; * Replacement of approximately 18 40-watt conventional fluorescent...

  20. CX-004575: Categorical Exclusion Determination | Department of...

    Broader source: Energy.gov (indexed) [DOE]

    approximately 277 conventional 60-watt light bulbs in tribal homes with 13-watt Light Emitting Diode (LED) bulbs; ? Replacement of approximately 18 40-watt conventional fluorescent...

  1. A novel fluorescence detection method for organosilanes

    SciTech Connect (OSTI)

    Yang, H.X.; Craw, M.T.; Depew, M.C.; Wan, J.K.S. (Queen's Univ., Kingston, Ontario (Canada))

    1989-02-01T23:59:59.000Z

    Quinones such as phenanthraquinone and anthraquinone react with organosilanes giving persistent, strongly fluorescent radicals. The reactions are photochemically initiated, rapid, and facile; detection of concentrations of silanes in the 10{sup {minus}7} M range are readily achieved. The staring materials do not fluorescence making the procedure simple, selective and specific.

  2. Applications of Nuclear Resonance Fluorescence

    SciTech Connect (OSTI)

    Warren, Glen A.; Detwiler, Rebecca S.; Peplowski, Patrick N.

    2010-11-11T23:59:59.000Z

    Nuclear Resonance Fluorescence (NRF) has the potential of addressing a wide variety of applications, which require isotopic and/or elemental information about a sample. We have investigated a variety of non-proliferation applications that may be addressed by NRF. From these applications, we have selected two, measuring uranium enrichment in UF6 cylinders and material verification in dismantlement, to investigate in more detail. Analytical models have been developed to evaluate these applications, and test measurements have been conducted to validate those models. We found that it is unlikely with current technology to address the requirements for UF6 cylinder enrichment measurements. In contrast, NRF is a very promising approach for material verification for dismantlement.

  3. Hard X-ray Fluorescence Measurements of Heteroepitaxial Solid...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Hard X-ray Fluorescence Measurements of Heteroepitaxial Solid Oxide Fuel Cell Cathode Materials. Hard X-ray Fluorescence Measurements of Heteroepitaxial Solid Oxide Fuel Cell...

  4. Using Nuclear Resonance Fluorescence for Nondestructive Isotopic Analysis

    E-Print Network [OSTI]

    Ludewigt, Bernhard A.

    2010-01-01T23:59:59.000Z

    7, (1959) pp. 54. [12] B.J. Quiter, ``Nuclear ResonanceFluorescence for Nuclear Materials Assay,'' University ofclandestine material with nuclear resonance fluorescence,"

  5. Super-Resolution Fluorescence Nanoscopy Applied to Image Core...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Super-Resolution Fluorescence Nanoscopy Applied to Image Core-Shell Photoswitching Nanoparticles and their Self-Assemblies . Super-Resolution Fluorescence Nanoscopy Applied to...

  6. Glucose sensing molecules having selected fluorescent properties

    DOE Patents [OSTI]

    Satcher, Jr., Joe H.; Lane, Stephen M.; Darrow, Christopher B.; Cary, Douglas R.; Tran, Joe Anh

    2004-01-27T23:59:59.000Z

    An analyte sensing fluorescent molecule that employs intramolecular electron transfer is designed to exhibit selected fluorescent properties in the presence of analytes such as saccharides. The selected fluorescent properties include excitation wavelength, emission wavelength, fluorescence lifetime, quantum yield, photostability, solubility, and temperature or pH sensitivity. The compound comprises an aryl or a substituted phenyl boronic acid that acts as a substrate recognition component, a fluorescence switch component, and a fluorophore. The fluorophore and switch component are selected such that the value of the free energy for electron transfer is less than about 3.0 kcal mol.sup.-1. Fluorescent compounds are described that are excited at wavelengths greater than 400 nm and emit at wavelengths greater than 450 nm, which is advantageous for optical transmission through skin. The fluorophore is typically selected from transition metal-ligand complexes and thiazine, oxazine, oxazone, or oxazine-one as well as anthracene compounds. The fluorescent compound can be immobilized in a glucose permeable biocompatible polymer matrix that is implantable below the skin.

  7. Hiding patterns with daylight fluorescent inks

    E-Print Network [OSTI]

    Romain Rossier; Roger D. Hersch; School Of Computer; Communication Sciences; Ecole Polytechnique; Fédérale Lausanne

    We propose a method for hiding patterns within printed images by making use of classical and of two daylight fluorescent magenta and yellow inks. Under the D65 illuminant we establish in the CIELAB space the gamut of a classical cmyk printer and the gamut of the same printer using a combination of classical inks with daylight fluorescent inks. These gamuts show that a significant part of the classical ink gamut can be reproduced by combining classical inks with daylight fluorescent inks. By printing parts of images with a combination of classical and daylight fluorescent inks instead of using classical inks only, we can hide security patterns within printed images. Under normal daylight, we do not see any difference between the parts printed with classical inks only and the parts printed with daylight fluorescent inks and classical inks. By changing the illumination, e.g. by viewing the printed image under a tungsten lamp or under a UV lamp, the daylight fluorescent inks change their colors and reveal the security pattern formed by combinations of classical inks and of daylight fluorescent inks.

  8. Photosystem II fluorescence lifetime imaging in avocado leaves: Contributions of the lutein-epoxide and violaxanthin cycles to fluorescence quenching

    E-Print Network [OSTI]

    Govindjee

    Photosystem II fluorescence lifetime imaging in avocado leaves: Contributions of the lutein a fluorescence were made on leaves of avocado plants to study whether rapidly reversible Dp

  9. Sustainable LED Fluorescent Light Replacement Technology

    SciTech Connect (OSTI)

    None

    2011-06-30T23:59:59.000Z

    Ilumisys and the National Center for Manufacturing Sciences (NCMS) partnered on a three-year project awarded by the United States (U.S.) Department of Energy (DOE), to quantify the impacts of LED lamps, incandescent lamps and fluorescent benchmark lamps over a product lifecycle – i.e. to develop a sustainable design and manufacturing strategy that addresses product manufacturing, use, recycling and disposal scenarios for LED-based lighting. Based on the knowledge gained from extensive product tear-down studies of fluorescent and screw-in lighting products, lifecycle assessment tools, and accelerated lifecycle testing protocols, an interactive Sustainable LED Design Guide has been developed to aid architectural and lighting designers and engineers in making design decisions that consider three important environmental impacts (greenhouse gas emissions, energy use and mercury emission) across all phases of the life of an LED lighting product. Critical information developed for the lifecycle analysis and product feature comparisons is the useful life of the lighting product as well as its performance. The Design Guide is available at www.ncms.org, and was developed based on operational and durability testing of a variety of lighting products including power consumption, light output, and useful life of a lamp in order to allow a more realistic comparison of lamp designs. This report describes the main project tasks, results and innovative features of the lifecycle assessment (LCA)-based design tools, and the key considerations driving the sustainable design of LED lighting systems. The Design Guide incorporates the following three novel features for efficiently evaluating LED lighting features in value-chains: • Bill-of-Materials (BOM) Builder – Designers may import process data for each component and supply functional data for the product, including power, consumption, lumen output and expected useful life. • Environmental Impact Review – Designs are comparable across lifecycle phases, subsystems, and environmental impact category, and can be normalized to a userdefined functional unit. • Drill-down Review – These provide an indepth look at individual lamp designs with the ability to review across subsystem or lifecycle phase.

  10. Probing the denatured state ensemble with fluorescence

    E-Print Network [OSTI]

    Alston, Roy Willis

    2004-09-30T23:59:59.000Z

    around the original tryptophan substitutions in RNase Sa. Regardless of the denaturant, ?max for the proteins and model compounds differed very little, 349.3 ± 1.2 nm. However, significant differences were observed in the fluorescence intensity at ?max...

  11. Fluorescence Imaging for Nuclear Arms Control Verification

    E-Print Network [OSTI]

    Feener, Jessica S

    2014-08-14T23:59:59.000Z

    treaties. Specifically, this technique uses fluorescence imaging to determine fissile material attributes in verifying, during the dismantlement process, an uncanned nuclear warhead or warhead component without revealing sensitive information. This could...

  12. Fluorescence-based optical glucose sensing 

    E-Print Network [OSTI]

    Meledeo, Michael Adam

    2002-01-01T23:59:59.000Z

    Previous studies have indicated that optical means of blood glucose detection are feasible and provide a minimally invasive alternative to current commercially available modalities. Fluorescence spectroscopy has shown promise in many examples...

  13. Using Nuclear Resonance Fluorescence for Nondestructive Isotopic Analysis

    E-Print Network [OSTI]

    Ludewigt, Bernhard A.

    2010-01-01T23:59:59.000Z

    spent fuel assay using Nuclear Resonance Fluorescence,” Annual Meeting of the Institute of Nuclear Materials Management,

  14. Multiplexed fluorescence detector system for capillary electrophoresis

    DOE Patents [OSTI]

    Yeung, E.S.; Taylor, J.A.

    1994-06-28T23:59:59.000Z

    A fluorescence detection system for capillary electrophoresis is provided wherein the detection system can simultaneously excite fluorescence and substantially simultaneously monitor separations in multiple capillaries. This multiplexing approach involves laser irradiation of a sample in a plurality of capillaries through optical fibers that are coupled individually with the capillaries. The array is imaged orthogonally through a microscope onto a charge-coupled device camera for signal analysis. 14 figures.

  15. Multiplexed fluorescence detector system for capillary electrophoresis

    DOE Patents [OSTI]

    Yeung, E.S.; Taylor, J.A.

    1996-03-12T23:59:59.000Z

    A fluorescence detection system for capillary electrophoresis is provided wherein the detection system can simultaneously excite fluorescence and substantially simultaneously monitor separations in multiple capillaries. This multiplexing approach involves laser irradiation of a sample in a plurality of capillaries through optical fibers that are coupled individually with the capillaries. The array is imaged orthogonally through a microscope onto a charge-coupled device camera for signal analysis. 14 figs.

  16. Ultratrace analysis of transuranic actinides by laser-induced fluorescence

    DOE Patents [OSTI]

    Miller, S.M.

    1983-10-31T23:59:59.000Z

    Ultratrace quantities of transuranic actinides are detected indirectly by their effect on the fluorescent emissions of a preselected fluorescent species. Transuranic actinides in a sample are coprecipitated with a host lattice material containing at least one preselected fluorescent species. The actinide either quenches or enhances the laser-induced fluorescence of the preselected fluorescent species. The degree of enhancement or quenching is quantitatively related to the concentration of actinide in the sample.

  17. Determination of the transport and change in composition of fluorescent materials in hydrologic systems by use of EEM spectroscopy

    SciTech Connect (OSTI)

    Goldberg, M.C. (Geological Survey, Lakewood, CO (USA))

    1990-01-01T23:59:59.000Z

    EEM spectra are sufficiently selective to allow fluorescence pattern characterization of the organic load of a river at a given location and to follow, qualitatively, the changes that ensue in that load as it moves in the river. By comparing the patterns of the load to specific materials such as illustrated by the Suwannee River pattern, humic acid fraction, comparison; it is possible to label major fluorescing components and to discern their appearance and disappearance as a function of location.

  18. A fluorescent assay for chloride transport; identification of a synthetic anionophore with improved activity

    E-Print Network [OSTI]

    Smith, Bradley D.

    A fluorescent assay for chloride transport; identification of a synthetic anionophore with improved on the chloride-sensitive probe, lucigenin, is developed for monitoring chloride transport into vesicles, and used to compare the effectiveness of three steroid- derived transporters. A topic of growing interest

  19. Remote temperature measurements in femto-liter volumes using dual-focus-Fluorescence Correlation Spectroscopy

    E-Print Network [OSTI]

    Enderlein, Jörg

    Remote temperature measurements in femto-liter volumes using dual-focus-Fluorescence Correlation February 2009 DOI: 10.1039/b807910b Remote temperature measurements in microfluidic devices with micrometer for remote temperature measurements on a micrometer scale. We perform comparative temperature measurements

  20. A portable time-domain LED fluorimeter for nanosecond fluorescence lifetime measurements

    SciTech Connect (OSTI)

    Wang, Hongtao; Salthouse, Christopher D., E-mail: salthouse@ecs.umass.edu [Electrical and Computer Engineering Department, University of Massachusetts, Amherst, Massachusetts 01003 (United States); Center for Personalized Health Monitoring, University of Massachusetts, Amherst, Massachusetts 01003 (United States); Qi, Ying; Mountziaris, T. J. [Center for Personalized Health Monitoring, University of Massachusetts, Amherst, Massachusetts 01003 (United States) [Center for Personalized Health Monitoring, University of Massachusetts, Amherst, Massachusetts 01003 (United States); Chemical Engineering Department, University of Massachusetts, Amherst, Massachusetts 01003 (United States)

    2014-05-15T23:59:59.000Z

    Fluorescence lifetime measurements are becoming increasingly important in chemical and biological research. Time-domain lifetime measurements offer fluorescence multiplexing and improved handling of interferers compared with the frequency-domain technique. In this paper, an all solid-state, filterless, and highly portable light-emitting-diode based time-domain fluorimeter (LED TDF) is reported for the measurement of nanosecond fluorescence lifetimes. LED based excitation provides more wavelengths options compared to laser diode based excitation, but the excitation is less effective due to the uncollimated beam, less optical power, and longer latency in state transition. Pulse triggering and pre-bias techniques were implemented in our LED TDF to improve the peak optical power to over 100 mW. The proposed pulsing circuit achieved an excitation light fall time of less than 2 ns. Electrical resetting technique realized a time-gated photo-detector to remove the interference of the excitation light with fluorescence. These techniques allow the LED fluorimeter to accurately measure the fluorescence lifetime of fluorescein down to concentration of 0.5 ?M. In addition, all filters required in traditional instruments are eliminated for the non-attenuated excitation/emission light power. These achievements make the reported device attractive to biochemical laboratories seeking for highly portable lifetime detection devices for developing sensors based on fluorescence lifetime changes. The device was initially validated by measuring the lifetimes of three commercial fluorophores and comparing them with reported lifetime data. It was subsequently used to characterize a ZnSe quantum dot based DNA sensor.

  1. Supercontinuum Stimulated Emission Depletion Fluorescence Lifetime Imaging

    SciTech Connect (OSTI)

    Lesoine, Michael; Bose, Sayantan; Petrich, Jacob; Smith, Emily

    2012-06-13T23:59:59.000Z

    Supercontinuum (SC) stimulated emission depletion (STED) fluorescence lifetime imaging is demonstrated by using time-correlated single-photon counting (TCSPC) detection. The spatial resolution of the developed STED instrument was measured by imaging monodispersed 40-nm fluorescent beads and then determining their fwhm, and was 36 ± 9 and 40 ± 10 nm in the X and Y coordinates, respectively. The same beads measured by confocal microscopy were 450 ± 50 and 430 ± 30 nm, which is larger than the diffraction limit of light due to underfilling the microscope objective. Underfilling the objective and time gating the signal were necessary to achieve the stated STED spatial resolution. The same fluorescence lifetime (2.0 ± 0.1 ns) was measured for the fluorescent beads by using confocal or STED lifetime imaging. The instrument has been applied to study Alexa Fluor 594-phalloidin labeled F-actin-rich projections with dimensions smaller than the diffraction limit of light in cultured cells. Fluorescence lifetimes of the actin-rich projections range from 2.2 to 2.9 ns as measured by STED lifetime imaging.

  2. Ultrabright fluorescent OLEDS using triplet sinks

    DOE Patents [OSTI]

    Zhang, Yifan; Forrest, Stephen R; Thompson, Mark

    2013-06-04T23:59:59.000Z

    A first device is provided. The first device further comprises an organic light emitting device. The organic light emitting device further comprises an anode, a cathode, and an emissive layer disposed between the anode and the cathode. The emissive layer further comprises an organic host compound, an organic emitting compound capable of fluorescent emission at room temperature, and an organic dopant compound. The triplet energy of the dopant compound is lower than the triplet energy of the host compound. The dopant compound does not strongly absorb the fluorescent emission of the emitting compound.

  3. Design, Syntheses and Applications of Fluorescent Dyes

    E-Print Network [OSTI]

    Wu, Liangxing

    2010-10-12T23:59:59.000Z

    .11. Normalized UV absorbance and fluorescence of 1h and the labeled streptavidin 1s in 0.1 M lithium phosphate buffer, pH 7.4 ..................... 54 3.1. Spectra of BODIPYs 9 in CH 2 Cl 2 ........................................................... 65...) ............................................................ 94 4.8. Dependence of electronic spectra of 15h on pH ..................................... 96 4.9. UV-Vis absorption and fluorescence spectra of rosamine 25 and 25 labeled avidin in 0.1 M phosphate buffer, pH 7...

  4. A fluorescence-based method for rapid and direct determination of Polybrominated Diphenyl Ethers in Water

    SciTech Connect (OSTI)

    Shan, Huimei; Liu, Chongxuan; Wang, Zheming; Ma, Teng; Shang, Jianying; Pan, Duoqiang

    2015-01-01T23:59:59.000Z

    A new method was developed for rapid and direct measurement of aqueous polybrominated diphenyl ethers (PBDEs) using fluorescence spectroscopy. The fluorescence spectra of aqueous tri- to deca-BDE (BDE28, 47, 99, 153, 190 and 209) that are commonly found in environment were determined at variable emission and excitation wavelengths. The results revealed that the PBDEs have distinct fluorescence spectral profiles and peak positions that can be exploited to identify these species and determine their concentrations in aqueous solutions. The detection limits as determined in deionized water spiked with PBDEs are 1.71-5.82 ng/L for BDE28, BDE47, BDE190 and BDE209, and 45.55-69.95 ng/L for BDE99 and BDE153. The effects of environmental variables including pH, humic substance, and groundwater chemical composition on PBDEs measurements were also investigated. These environmental variables affected fluorescence intensity, but their effect can be corrected through linear additivity and separation of spectral signal contribution. Compared with conventional GC-based analytical methods, the fluorescence spectroscopy method is more efficient as it only uses a small amount of samples (4 ml), avoids lengthy complicated concentration and extraction steps, and has a low detection limit of a few ng/L.

  5. CODED SPECTROSCOPY FOR ETHANOL DETECTION IN DIFFUSE, FLUORESCENT MEDIA

    E-Print Network [OSTI]

    ABSTRACT CODED SPECTROSCOPY FOR ETHANOL DETECTION IN DIFFUSE, FLUORESCENT MEDIA by Scott Thomas Mc FOR ETHANOL DETECTION IN DIFFUSE, FLUORESCENT MEDIA by Scott Thomas McCain Department of Electrical

  6. Modelling of a Fluorescent Lamp Plasma

    E-Print Network [OSTI]

    Eindhoven, Technische Universiteit

    Technische Universiteit Eindhoven, op gezag van de Rector Magnificus, prof.dr. R.A. van Santen, voor een TECHNISCHE UNIVERSITEIT EINDHOVEN Hartgers, Albertus Modelling of a Fluorescent Lamp Plasma / by Albertus Hartgers. - Eindhoven : Technische Universiteit Eindhoven, 2003. - Proefschrift. ISBN 90-386-1665-1 NUR 924

  7. Oil Classification with Fluorescence Spectroscopy Engineering Physics

    E-Print Network [OSTI]

    Oldenburg, Carl von Ossietzky Universität

    detected by these channels. The investigation used three methods to examine crude oil, heavy oil, sludge1 Oil Classification with Fluorescence Spectroscopy Engineering Physics Master of Engineering and classification of oil spills on water surfaces. It is an overview of the laser remote sensor technique

  8. DNA sequencing using fluorescence background electroblotting membrane

    DOE Patents [OSTI]

    Caldwell, Karin D. (Salt Lake City, UT); Chu, Tun-Jen (Salt Lake City, UT); Pitt, William G. (Orem, UT)

    1992-01-01T23:59:59.000Z

    A method for the multiplex sequencing on DNA is disclosed which comprises the electroblotting or specific base terminated DNA fragments, which have been resolved by gel electrophoresis, onto the surface of a neutral non-aromatic polymeric microporous membrane exhibiting low background fluorescence which has been surface modified to contain amino groups. Polypropylene membranes are preferably and the introduction of amino groups is accomplished by subjecting the membrane to radio or microwave frequency plasma discharge in the presence of an aminating agent, preferably ammonia. The membrane, containing physically adsorbed DNA fragments on its surface after the electroblotting, is then treated with crosslinking means such as UV radiation or a glutaraldehyde spray to chemically bind the DNA fragments to the membrane through said smino groups contained on the surface thereof. The DNA fragments chemically bound to the membrane are subjected to hybridization probing with a tagged probe specific to the sequence of the DNA fragments. The tagging may be by either fluorophores or radioisotopes. The tagged probes hybridized to said target DNA fragments are detected and read by laser induced fluorescence detection or autoradiograms. The use of aminated low fluorescent background membranes allows the use of fluorescent detection and reading even when the available amount of DNA to be sequenced is small. The DNA bound to the membrances may be reprobed numerous times.

  9. Red phosphors for use in high CRI fluorescent lamps

    DOE Patents [OSTI]

    Srivastava, Alok; Comanzo, Holly; Manivannan, Vankatesan; Setlur, Anant Achyut

    2005-11-15T23:59:59.000Z

    Novel red emitting phosphors for use in fluorescent lamps resulting in superior color rendering index values compared to conventional red phosphors. Also disclosed is a fluorescent lamp including a phosphor layer comprising blends of one or more of a blue phosphor, a blue-green phosphor, a green phosphor and a red a phosphor selected from the group consisting of SrY.sub.2 O.sub.4 :Eu.sup.3+, (Y,Gd)Al.sub.3 B.sub.4 O.sub.12 :Eu.sup.3+, and [(Y.sub.1-x-y-m La.sub.y)Gd.sub.x ]BO.sub.3 :Eu.sub.m wherein y<0.50 and m=0.001-0.3. The phosphor layer can optionally include an additional deep red phosphor and a yellow emitting phosphor. The resulting lamp will exhibit a white light having a color rendering index of 90 or higher with a correlated color temperature of from 2500 to 10000 Kelvin. The use of the disclosed red phosphors in phosphor blends of lamps results in high CRI light sources with increased stability and acceptable lumen maintenance over the course of the lamp life.

  10. Student Directions Student Directions: Using your homework from "Building a Fancy Spectrograph" fill in the

    E-Print Network [OSTI]

    Mojzsis, Stephen J.

    Incandescent Bulb Incandescent Bulb Florescent Bulb #12;_________________________________________________________________________________ 7. How does the spectrum from the frosted bulb compare to that of the unfrosted bulb? 8. From number 7, what can you conclude about the material that makes the bulb "frosted?" 9. How is the florescent

  11. Laser-excited atomic fluorescence of atoms produced in a graphite furnace

    SciTech Connect (OSTI)

    Goforth, D.; Winefordner, J.D.

    1986-11-01T23:59:59.000Z

    Laser-excited atomic fluorescence in a graphite furnace gives detection limits for Pb, Cu, Mn, Sn, Al, In, Li, and Pt, in the picogram to sub-picogram range. The linear dynamic range for these elements varies from 3 to 7 orders of magnitude. A graphite rod, a plain graphite cup, and a slotted graphite cup are compared as the cuvette in the fluorescence system. Detection limits for a pyrolytic coating, a tantalum foil liner, and a tantalum carbide coating of the graphite cuvette are compared. A hydrogen-argon atmosphere, a low-pressure atmosphere, and an argon atmosphere are compared as the atmosphere surrounding the graphite cuvette. Lastly, Cu and Mn are determined in several standard reference materials.

  12. Text-Alternative Version: LED Replacements for Linear Fluorescent Lamps

    Broader source: Energy.gov [DOE]

    Below is the text-alternative version of the "LED Replacements for Linear Fluorescent Lamps" webcast, held June 20, 2011.

  13. Confocal, FLIM & Multi-Photon Fluorescence Microscope | EMSL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    supports quantitative fluorescence resonance energy transfer (FRET) for investigating molecular interaction dynamics in living cells. The system also includes differential...

  14. The effect of a synthetic cytokinin, 6-benzylaminopurine, and light quality on Ficus benjamina under low light intensities

    E-Print Network [OSTI]

    Meadows, Sylvia Elise

    1979-01-01T23:59:59.000Z

    that 1, 100 ft-c of tungsten light would give a higher dry weight gain than 1, 000 ft-c fluorescent, 1, 100 ft-c fluorescent plus tungsten, or 1, 800 ft-c of fluorescent plus tungsten plus mercury. In beans Leiser et. al, found that tungsten gave a... higher dry weight gain than fluorescent plus tungsten plus mercury or fluorescent plus mercury, or fluor scent alone (23). Incandescent bulbs are high in infrared irradiation compared to fluorescent light sources, and it has been found that infrared 1r...

  15. Measurement and model assessment of fluorescence lifetime sensing in multiply scattering media 

    E-Print Network [OSTI]

    Kuwana, Eddy

    2005-08-29T23:59:59.000Z

    fluorescence sensor and the reliability of fluorescence lifetime measurement verify the prospect of this technology for implantable purposes....

  16. what goes here? Beverages

    E-Print Network [OSTI]

    de Lijser, Peter

    · Chemicals · Solvents (gas,antifreeze) · Fluorescent light tubes & bulbs · Lubricants & motor oil · Oil

  17. Performance of T12 and T8 Fluorescent Lamps and Troffers and LED Linear Replacement Lamps CALiPER Benchmark Report

    SciTech Connect (OSTI)

    Myer, Michael; Paget, Maria L.; Lingard, Robert D.

    2009-01-16T23:59:59.000Z

    The Department of Energy (DOE) Commercially Available LED Product Evaluation and Reporting (CALiPER) Program was established in 2006 to investigate the performance of light-emitting diode (LED) based luminaires and replacement lamps. To help users better compare LED products with conventional lighting technologies, CALiPER has also performed benchmark research and testing of traditional (i.e., non-LED) lamps and fixtures. This benchmark report addresses standard 4-foot fluorescent lamps (i.e., T12 and T8) and the 2-foot by 4-foot recessed troffers in which they are commonly used. This report also examines available LED replacements for T12 and T8 fluorescent lamps, and their application in fluorescent troffers. The construction and operation of linear fluorescent lamps and troffers are discussed, as well as fluorescent lamp and fixture performance, based on manufacturer data and CALiPER benchmark testing. In addition, the report describes LED replacements for linear fluorescent lamps, and compares their bare lamp and in situ performance with fluorescent benchmarks on a range of standard lighting measures, including power usage, light output and distribution, efficacy, correlated color temperature, and the color rendering index. Potential performance and application issues indicated by CALiPER testing results are also examined.

  18. Design and Predictive Control of a Net Zero Energy Home

    E-Print Network [OSTI]

    Morelli, F.; Abbarno, N.; Boese, E.; Bullock, J.; Carter, B.; Edwards, R.; Lapite, O.; Mann, D.; Mulvihill, C.; Purcell, E.; Stein, M. IV; Rasmussen, B. P.

    2013-01-01T23:59:59.000Z

    the same amount of light as traditional incandescent bulbs with less energy. Incandescent bulbs are inherently inefficient as most of the energy they consume goes towards heat generation. Compact fluorescent (CFL) and light emitting diode (LED) bulbs... as heat [1]. Compact fluorescent lamps (CFLs) and Light Emitting Diodes (LEDs) were analyzed in comparison with incandescent lamps. To determine the most energy efficient bulb, energy consumption for each type of bulb is needed. To do this, the amount...

  19. Solid state laser media driven by remote nuclear powered fluorescence

    DOE Patents [OSTI]

    Prelas, Mark A. (Columbia, MO)

    1992-01-01T23:59:59.000Z

    An apparatus is provided for driving a solid state laser by a nuclear powered fluorescence source which is located remote from the fluorescence source. A nuclear reaction produced in a reaction chamber generates fluorescence or photons. The photons are collected from the chamber into a waveguide, such as a fiber optic waveguide. The waveguide transports the photons to the remote laser for exciting the laser.

  20. Absolute Calibration of the Auger Fluorescence Detectors

    E-Print Network [OSTI]

    P. Bauleo; J. Brack; L. Garrard; J. Harton; R. Knapik; R. Meyhandan; A. C. Rovero; A. Tamashiro; D. Warner; for the Auger Collaboration

    2005-07-14T23:59:59.000Z

    Absolute calibration of the Pierre Auger Observatory fluorescence detectors uses a light source at the telescope aperture. The technique accounts for the ombined effects of all detector components in a single measurement. The calibrated 2.5 m diameter light source fills the aperture, providing uniform illumination to each pixel. The known flux from the light source and the response of the acquisition system give the required calibration for each pixel. In the lab, light source uniformity is studied using CCD images and the intensity is measured relative to NIST-calibrated photodiodes. Overall uncertainties are presently 12%, and are dominated by systematics.

  1. Fluorescent Lamp Ballasts | Department of Energy

    Energy Savers [EERE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directed offOCHCO2: FinalOffers3.pdf0-45.pdf Flash2010-45.pdfFlash2011-43and Statement ofStrikeFluorescent

  2. Fluorescent Optical Position Sensor - Energy Innovation Portal

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOEThe Bonneville Power AdministrationField8,Dist.New MexicoFinancingProofWorking OutsideFluorescent

  3. Fluorescent Pigments for High-Performance Cool Roofing and Facades...

    Energy Savers [EERE]

    dark-colored pigments for cool metal roof and faade coatings that incorporate near-infrared fluorescence and reflectance to improve energy performance. Image: PPG Industries 2...

  4. Satellite-detected fluorescence reveals global physiology of ocean phytoplankton

    E-Print Network [OSTI]

    2009-01-01T23:59:59.000Z

    2000. Jassby, A. D. and Platt, T. : Mathematical formulationTopliss, B. J. and Platt, T. : Passive fluorescence andrates (Topliss and Platt, 1986; Kiefer et al. , 1989) and

  5. atomic fluorescence spectrometry: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    S. Chapman 2010-12-08 9 Quantification of trace metals in deciduous tooth enamel using energy dispersive X-ray fluorescence spectrometry. Open Access Theses and Dissertations...

  6. Fluorescent Multiblock ?-Conjugated Polymer Nanoparticles for In Vivo Tumor Targeting

    E-Print Network [OSTI]

    Ahmed, Eilaf

    Highly fluorescent multiblock conjugated polymer nanoparticles with folic acid surface ligands are highly effective for bioimaging and in vivo tumor targeting. The targeted nanoparticles were preferentially localized in ...

  7. Time-resolved fluorescence decay measurements for flowing particles

    DOE Patents [OSTI]

    Deka, C.; Steinkamp, J.A.

    1999-06-01T23:59:59.000Z

    Time-resolved fluorescence decay measurements are disclosed for flowing particles. An apparatus and method for the measurement and analysis of fluorescence for individual cells and particles in flow are described, wherein the rapid measurement capabilities of flow cytometry and the robust measurement and analysis procedures of time-domain fluorescence lifetime spectroscopy are combined. A pulse-modulated CW laser is employed for excitation of the particles. The characteristics and the repetition rate of the excitation pulses can be readily adjusted to accommodate for fluorescence decays having a wide range of lifetimes. 12 figs.

  8. aequorea green fluorescent: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    plants. Jim Haseloff Biology and Medicine Websites Summary: light, and an accessory green fluorescent protein (GFP), which accepts energy from aequorin and reImaging green...

  9. Modeling of highly loaded fluorescent lamps

    SciTech Connect (OSTI)

    Lister, G.G.; Lawler, J.E.; Curry, J.J.

    1999-07-01T23:59:59.000Z

    Numerical modeling of the positive column of fluorescent lamps under conditions of high current density are of current interest, particularly in view of recent developments in electrodeless lamps. Current models tend to overestimate radiation output, and consequently the maintenance electric field in these discharges. Under highly loaded conditions, mercury-rare gas fluorescent lamps exhibit strong mercury depletion on axis (cataphoresis), and an understanding of resonance radiation transport under these conditions is therefore vital to the development of models with a predictive capability. The authors have explored the effect of radial cataphoresis on resonance radiation trapping for situations in which the radiation transport is dominated by foreign gas broadening, Doppler broadening, or resonance collisional broadening of the spectral line. Several different production rates per unit volume of resonance (excited) atoms have also been studied. It is advantageous in many cases to parameterize the trapped decay rate in terms of the total number of ground state atoms in the positive column independent of their radial distribution. The results of this work have been included in a numerical model of the positive column and the predicted influence on discharge parameters will be presented for cases of interest to highly loaded lamps.

  10. Max Tech and Beyond: Fluorescent Lamps

    SciTech Connect (OSTI)

    Scholand, Michael

    2012-04-01T23:59:59.000Z

    Fluorescent lamps are the most widely used artificial light source today, responsible for approximately 70% of the lumens delivered to our living spaces globally. The technology was originally commercialized in the 1930's, and manufacturers have been steadily improving the efficacy of these lamps over the years through modifications to the phosphors, cathodes, fill-gas, operating frequency, tube diameter and other design attributes. The most efficient commercially available fluorescent lamp is the 25 Watt T5 lamp. This lamp operates at 114-116 lumens per watt while also providing good color rendering and more than 20,000 hours of operating life. Industry experts interviewed indicated that while this lamp is the most efficient in the market today, there is still a further 10 to 14% of potential improvements that may be introduced to the market over the next 2 to 5 years. These improvements include further developments in phosphors, fill-gas, cathode coatings and ultraviolet (UV) reflective glass coatings. The commercialization of these technology improvements will combine to bring about efficacy improvements that will push the technology up to a maximum 125 to 130 lumens per watt. One critical issue raised by researchers that may present a barrier to the realization of these improvements is the fact that technology investment in fluorescent lamps is being reduced in order to prioritize research into light emitting diodes (LEDs) and ceramic metal halide high intensity discharge (HID) lamps. Thus, it is uncertain whether these potential efficacy improvements will be developed, patented and commercialized. The emphasis for premium efficacy will continue to focus on T5 lamps, which are expected to continue to be marketed along with the T8 lamp. Industry experts highlighted the fact that an advantage of the T5 lamp is the fact that it is 40% smaller and yet provides an equivalent lumen output to that of a T8 or T12 lamp. Due to its smaller form factor, the T5 lamp contains less material (i.e., glass, fill gas and phosphor), and has a higher luminance, enabling fixtures to take advantage of the smaller lamp size to improve the optics and provide more efficient overall system illuminance. In addition to offering the market a high-quality efficacious light source, another strong value proposition of fluorescent lighting is its long operating life. In today's market, one manufacturer is offering fluorescent lamps that have a rated life of 79,000 hours - which represents 18 years of service at 12 hours per day, 365 days per year. These lamps, operated using a long-life ballast specified by the manufacturer, take advantage of improvements in cathode coatings, fill gas chemistry and pressure to extend service life by a factor of four over conventional fluorescent lamps. It should be noted that this service life is also longer (approximately twice as long) as today's high-quality LED products. The fluorescent market is currently focused on the T5 and T8 lamp diameters, and it is not expected that other diameters would be introduced. Although T8 is a more optimal diameter from an efficacy perspective, the premium efficiency and optimization effort has been focused on T5 lamps because they are 40% smaller than T8, and are designed to operate at a higher temperature using high-frequency electronic ballasts. The T5 lamp offers savings in terms of materials, packaging and shipping, as well as smaller fixtures with improved optical performance. Manufacturers are actively researching improvements in four critical areas that are expected to yield additional efficacy improvements of approximately 10 to 14 percent over the next five years, ultimately achieving approximately 130 lumens per watt by 2015. The active areas of research where these improvements are anticipated include: (1) Improved phosphors which continue to be developed and patented, enabling higher efficacies as well as better color rendering and lumen maintenance; (2) Enhanced fill gas - adjusting proportions of argon, krypton, neon and xenon to optimize performance, while also m

  11. Development and Application of Fluorescent SDF-1 Derivatives Ryo Masuda,

    E-Print Network [OSTI]

    Takada, Shoji

    #12;1 Development and Application of Fluorescent SDF-1 Derivatives Ryo Masuda, Shinya Oishi of Medicine, 2-1 Seiryocho, Aoba-ku, Sendai 980-8575, Japan. #12;2 Abstract Background: SDF-1/CXCR4 signaling, structure-activity relationship analyses of fluorescent SDF-1 derivatives were carried out. Results: Several

  12. Improved ``Optical Highlighter'' Probes Derived from Discosoma Red Fluorescent Protein

    E-Print Network [OSTI]

    Marchant, Jonathan

    , Minneapolis, Minnesota ABSTRACT The tetrameric red fluorescent protein, DsRed, undergoes a rapid red to green-emitting species of DsRed and an enhancement of emission from the ``immature'' green species, likely caused by dequenching of fluorescence resonance energy transfer occurring within the protein tetramer. Here, we have

  13. Gregorio Weber International Prize in Biological Fluorescence 2008

    E-Print Network [OSTI]

    Weber, David J.

    Gregorio Weber International Prize in Biological Fluorescence 2008 The Gregorio Weber International Prize in Biological Fluorescence (Weber Prize) is awarded for research related to a doctoral (or finalists. Weber Prizes were awarded in 2002 and 2005. The third Weber Prize will be awarded in 2008

  14. Comparison of Segmentation Algorithms For Fluorescence Microscopy Images of Cells

    E-Print Network [OSTI]

    Bernal, Javier

    - mentation techniques are less accurate than k-means clustering with multiple clusters. Segmentation accuracy fluorescence microscopy; k-means cluster; image segmentation; cell edge; bivariate simi- larity index NUMEROUSComparison of Segmentation Algorithms For Fluorescence Microscopy Images of Cells Alden A. Dima,1

  15. Fluorescence correlation spectroscopy at high concentrations using gold bowtie nanoantennas

    E-Print Network [OSTI]

    Fan, Shanhui

    Fluorescence correlation spectroscopy at high concentrations using gold bowtie nanoantennas Anika A to extremely low concentrations. Plasmonic gold bowtie nanoantennas enhance a single molecule's fluorescence relative to a large background of unenhanced molecules, and here we show that bowties can extend FCS

  16. Teal fluorescent proteins: Characterization of a reversibly photoswitchable variant

    E-Print Network [OSTI]

    Campbell, Robert E.

    libraries of variants. mTFP1 has proven to be a particularly useful new member of the FP `toolbox green fluorescent protein (GFP) in 1961 when he was isolating aequorin, a calcium improved variants of GFP, including the variant known as enhanced green fluorescent protein (EGFP), were

  17. Application of Quantitative Fluorescence and Absorption-Edge

    E-Print Network [OSTI]

    Sparks, Donald L.

    Application of Quantitative Fluorescence and Absorption-Edge Computed Microtomography to Image, Chicago, Illinois 60637 This paper shows that synchrotron-based fluorescence and absorption-edge computed, which had a well-correlated metal coating. Absorption-edge CMT showed the three-dimensional distribution

  18. A matter of collection and detection for intraoperative and noninvasive near-infrared fluorescence molecular imaging: To see or not to see?

    SciTech Connect (OSTI)

    Zhu, Banghe; Rasmussen, John C.; Sevick-Muraca, Eva M., E-mail: Eva.Sevick@uth.tmc.edu [Center for Molecular Imaging, The Brown Foundation Institute of Molecular Medicine, The University of Texas Health Science Center, Houston, Texas 77030 (United States)

    2014-02-15T23:59:59.000Z

    Purpose: Although fluorescence molecular imaging is rapidly evolving as a new combinational drug/device technology platform for molecularly guided surgery and noninvasive imaging, there remains no performance standards for efficient translation of “first-in-humans” fluorescent imaging agents using these devices. Methods: The authors employed a stable, solid phantom designed to exaggerate the confounding effects of tissue light scattering and to mimic low concentrations (nM–pM) of near-infrared fluorescent dyes expected clinically for molecular imaging in order to evaluate and compare the commonly used charge coupled device (CCD) camera systems employed in preclinical studies and in human investigational studies. Results: The results show that intensified CCD systems offer greater contrast with larger signal-to-noise ratios in comparison to their unintensified CCD systems operated at clinically reasonable, subsecond acquisition times. Conclusions: Camera imaging performance could impact the success of future “first-in-humans” near-infrared fluorescence imaging agent studies.

  19. SIMULTANEOUS MEASUREMENT OF CIRCULAR DICHROISM AND FLUORESCENCE POLARIZATION ANISOTROPY.

    SciTech Connect (OSTI)

    SUTHERLAND,J.C.

    2002-01-19T23:59:59.000Z

    Circular dichroism and fluorescence polarization anisotropy are important tools for characterizing biomolecular systems. Both are used extensively in kinetic experiments involving stopped- or continuous flow systems as well as titrations and steady-state spectroscopy. This paper presents the theory for determining circular dichroism and fluorescence polarization anisotropy simultaneously, thus insuring the two parameters are recorded under exactly the same conditions and at exactly the same time in kinetic experiments. The approach to measuring circular dichroism is that used in almost all conventional dichrographs. Two arrangements for measuring fluorescence polarization anisotropy are described. One uses a single fluorescence detector and signal processing with a lock-in amplifier that is similar to the measurement of circular dichroism. The second approach uses classic ''T'' format detection optics, and thus can be used with conventional photon-counting detection electronics. Simple extensions permit the simultaneous measurement of the absorption and excitation intensity corrected fluorescence intensity.

  20. Radioiodine detector based on laser induced fluorescence

    DOE Patents [OSTI]

    McDonald, Jimmie R. (Upper Marlboro, MD); Baronavski, Andrew P. (Alexandria, VA)

    1980-01-01T23:59:59.000Z

    The invention involves the measurement of the concentration of the radioisotope .sup.129 I.sub.2 in the presence of a gas. The invention uses a laser to excite a sample of the .sup.129 I.sub.2 in a sample gas chamber and a reference sample of a known concentration of .sup.129 I.sub.2 in a reference gas chamber. The .sup.129 I.sub.2 in the sample and reference gas chamber each gives off fluorescence emissions which are received by photomultipliers which provide signals to a detector. The detector uses a ratioing technique to determine the concentration of .sup.129 I.sub.2 in the sample gas chamber.

  1. Fluorescence in nonlocal dissipative periodic structures

    E-Print Network [OSTI]

    Francesco Intravaia; Kurt Busch

    2015-02-24T23:59:59.000Z

    We present an approach for the description of fluorescence from optically active material embedded in layered periodic structures. Based on an exact electromagnetic Green's tensor analysis, we determine the radiative properties of emitters such as the local photonic density of states, Lamb shifts, line widths etc. for a finite or infinite sequence of thin alternating plasmonic and dielectric layers. In the effective medium limit, these systems may exhibit hyperbolic dispersion relations so that the large wave-vector characteristics of all constituents and processes become relevant. These include the finite thickness of the layers, the nonlocal properties of the constituent metals, and local-field corrections associated with an emitter's dielectric environment. In particular, we show that the corresponding effects are non-additive and lead to considerable modifications of an emitter's luminescence properties.

  2. X-Ray Fluorescence (XRF) Analysis of Obsidian Artifacts from Shoofly Ruin, Central Arizona

    E-Print Network [OSTI]

    Shackley, M. Steven

    1986-01-01T23:59:59.000Z

    X-RAY FLUORESCENCE (XRF) ANALYSIS OF OBSIDIAN ARTIFACTS FROM62:426-437. SOUTHWEST XRF PAPER Table 1. X-ray fluorescence

  3. Air Fluorescence Relevant for Cosmic-Ray Detection - Summary of the 5th Fluorescence Workshop, El Escorial 2007

    E-Print Network [OSTI]

    Fernando Arqueros; Joerg R. Hoerandel; Bianca Keilhauer

    2008-07-24T23:59:59.000Z

    High-energy cosmic rays with energies exceeding $10^{17}$ eV are frequently observed by measurements of the fluorescence light induced by air showers. A major contribution to the systematic uncertainties of the absolute energy scale of such experiments is the insufficient knowledge of the fluorescence light yield of electrons in air. The aim of the 5th Fluorescence Workshop was to bring together experimental and theoretical expertise to discuss the latest progress on the investigations of the fluorescence light yield. The results of the workshop will be reviewed as well as the present status of knowledge in this field. Emphasis is given to the fluorescence light yield important for air shower observations and its dependence on atmospheric parameters, like pressure, temperature, and humidity. The effects of the latest results on the light observed from air showers will be discussed.

  4. Split green fluorescent protein as a modular binding partner for protein crystallization

    SciTech Connect (OSTI)

    Nguyen, Hau B. [Los Alamos National Laboratory, MS M888, Los Alamos, NM 87545 (United States); Hung, Li-Wei [Los Alamos National Laboratory, MS D454, Los Alamos, NM 87545 (United States); Yeates, Todd O. [University of California, PO Box 951569, Los Angeles, CA 90095 (United States); Terwilliger, Thomas C., E-mail: terwilliger@lanl.gov; Waldo, Geoffrey S., E-mail: terwilliger@lanl.gov [Los Alamos National Laboratory, MS M888, Los Alamos, NM 87545 (United States)

    2013-12-01T23:59:59.000Z

    A strategy using a new split green fluorescent protein (GFP) as a modular binding partner to form stable protein complexes with a target protein is presented. The modular split GFP may open the way to rapidly creating crystallization variants. A modular strategy for protein crystallization using split green fluorescent protein (GFP) as a crystallization partner is demonstrated. Insertion of a hairpin containing GFP ?-strands 10 and 11 into a surface loop of a target protein provides two chain crossings between the target and the reconstituted GFP compared with the single connection afforded by terminal GFP fusions. This strategy was tested by inserting this hairpin into a loop of another fluorescent protein, sfCherry. The crystal structure of the sfCherry-GFP(10–11) hairpin in complex with GFP(1–9) was determined at a resolution of 2.6 Å. Analysis of the complex shows that the reconstituted GFP is attached to the target protein (sfCherry) in a structurally ordered way. This work opens the way to rapidly creating crystallization variants by reconstituting a target protein bearing the GFP(10–11) hairpin with a variety of GFP(1–9) mutants engineered for favorable crystallization.

  5. Portable total reflection x-ray fluorescence analysis in the identification of unknown laboratory hazards

    SciTech Connect (OSTI)

    Liu, Ying, E-mail: liu.ying.48r@st.kyoto-u.ac.jp; Imashuku, Susumu; Sasaki, Nobuharu; Ze, Long; Kawai, Jun [Department of Materials Science and Engineering, Kyoto University, Yoshida-Honmachi, Sakyo-ku, Kyoto 606-8501 (Japan); Takano, Shotaro; Sohrin, Yoshiki [Institute for Chemical Research, Kyoto University, Gokasho, Uji, Kyoto 611-0011 (Japan); Seki, Hiroko; Miyauchi, Hiroya [Kyoto Prefectural Technology Center for Small and Medium Enterprises, Chudojiminami machi, Shimogyo-ku, Kyoto 600-8813 (Japan)

    2014-05-15T23:59:59.000Z

    In this study, a portable total reflection x-ray fluorescence (TXRF) spectrometer was used to analyze unknown laboratory hazards that precipitated on exterior surfaces of cooling pipes and fume hood pipes in chemical laboratories. With the aim to examine the accuracy of TXRF analysis for the determination of elemental composition, analytical results were compared with those of wavelength-dispersive x-ray fluorescence spectrometry, scanning electron microscope and energy-dispersive x-ray spectrometry, energy-dispersive x-ray fluorescence spectrometry, inductively coupled plasma atomic emission spectrometry, x-ray diffraction spectrometry (XRD), and x-ray photoelectron spectroscopy (XPS). Detailed comparison of data confirmed that the TXRF method itself was not sufficient to determine all the elements (Z?>?11) contained in the samples. In addition, results suggest that XRD should be combined with XPS in order to accurately determine compound composition. This study demonstrates that at least two analytical methods should be used in order to analyze the composition of unknown real samples.

  6. Integrated ultrasonic particle positioning and low excitation light fluorescence imaging

    SciTech Connect (OSTI)

    Bernassau, A. L.; Al-Rawhani, M.; Beeley, J.; Cumming, D. R. S. [School of Engineering, University of Glasgow, Glasgow, G12 8LT (United Kingdom)] [School of Engineering, University of Glasgow, Glasgow, G12 8LT (United Kingdom)

    2013-12-09T23:59:59.000Z

    A compact hybrid system has been developed to position and detect fluorescent micro-particles by combining a Single Photon Avalanche Diode (SPAD) imager with an acoustic manipulator. The detector comprises a SPAD array, light-emitting diode (LED), lenses, and optical filters. The acoustic device is formed of multiple transducers surrounding an octagonal cavity. By stimulating pairs of transducers simultaneously, an acoustic landscape is created causing fluorescent micro-particles to agglomerate into lines. The fluorescent pattern is excited by a low power LED and detected by the SPAD imager. Our technique combines particle manipulation and visualization in a compact, low power, portable setup.

  7. Ultrahigh resolution multicolor colocalization of single fluorescent probes

    DOE Patents [OSTI]

    Weiss, Shimon; Michalet, Xavier; Lacoste, Thilo D.

    2005-01-18T23:59:59.000Z

    A novel optical ruler based on ultrahigh-resolution colocalization of single fluorescent probes is described. Two unique families of fluorophores are used, namely energy-transfer fluorescent beads and semiconductor nanocrystal (NC) quantum dots, that can be excited by a single laser wavelength but emit at different wavelengths. A novel multicolor sample-scanning confocal microscope was constructed which allows one to image each fluorescent light emitter, free of chromatic aberrations, by scanning the sample with nanometer scale steps using a piezo-scanner. The resulting spots are accurately localized by fitting them to the known shape of the excitation point-spread-function of the microscope.

  8. Fluorescent lamp unit with magnetic field generating means

    DOE Patents [OSTI]

    Grossman, M.W.; George, W.A.

    1989-08-08T23:59:59.000Z

    A fluorescent lamp unit having a magnetic field generating means for improving the performance of the fluorescent lamp is disclosed. In a preferred embodiment the fluorescent lamp comprises four longitudinally extending leg portions disposed in substantially quadrangular columnar array and joined by three generally U-shaped portions disposed in different planes. In another embodiment of the invention the magnetic field generating means comprises a plurality of permanent magnets secured together to form a single columnar structure disposed within a centrally located region defined by the shape of lamp envelope. 4 figs.

  9. Mechanical code comparator

    DOE Patents [OSTI]

    Peter, Frank J. (Albuquerque, NM); Dalton, Larry J. (Bernalillo, NM); Plummer, David W. (Albuquerque, NM)

    2002-01-01T23:59:59.000Z

    A new class of mechanical code comparators is described which have broad potential for application in safety, surety, and security applications. These devices can be implemented as micro-scale electromechanical systems that isolate a secure or otherwise controlled device until an access code is entered. This access code is converted into a series of mechanical inputs to the mechanical code comparator, which compares the access code to a pre-input combination, entered previously into the mechanical code comparator by an operator at the system security control point. These devices provide extremely high levels of robust security. Being totally mechanical in operation, an access control system properly based on such devices cannot be circumvented by software attack alone.

  10. Comparing greenhouse gasses

    E-Print Network [OSTI]

    Reilly, John M.; Babiker, Mustafa H.M.; Mayer, Monika.

    Controlling multiple substances that jointly contribute to climate warming requires some method to compare the effects of the different gases because the physical properties (radiative effects, and persistence in the ...

  11. A dosimetry study for a K-fluorescent x-ray system

    E-Print Network [OSTI]

    Beard, Travis Newton

    1975-01-01T23:59:59.000Z

    spectra from a Si(Li) semi-conductor radiation detector. The spectrum for the copper target indicated a considerable amount of scattered radiation from thc target, as compared to that produced by the other targets. Lip thcrmoluminesccnt dosimeters (TLD... of ionizing radiation detectors. The photon response of many detectors varies with the incident photon energy, especially in the 10-100 keV energy region. The proper choice of K-fluorescent X-ray radiators enables one to make very accurate energy...

  12. Metal enhanced fluorescence in rare earth doped plasmonic core-shell nanoparticles

    E-Print Network [OSTI]

    Derom, S; Pillonnet, A; Benamara, O; Jurdyc, A M; Girard, C; Francs, G Colas des

    2013-01-01T23:59:59.000Z

    We theoretically and numerically investigate metal enhanced fluorescence of plasmonic core-shell nanoparticles doped with rare earth (RE) ions. Particle shape and size are engineered to maximize the average enhancement factor (AEF) of the overall doped shell. We show that the highest enhancement (11 in the visible and 7 in the near-infrared) are achieved by tuning either the dipolar or quadrupolar particle resonance to the rare earth ions excitation wavelength. Additionally, the calculated AEFs are compared to experimental data reported in the literature, obtained in similar conditions (plasmon mediated enhancement) or when a metal-RE energy transfer mechanism is involved.

  13. FY08 Annual Report for Nuclear Resonance Fluorescence Imaging

    SciTech Connect (OSTI)

    Warren, Glen A.; Caggiano, Joseph A.

    2009-01-06T23:59:59.000Z

    FY08 annual report for project the "Nuclear Resonance Fluorescence Imaging" project. Reviews accomplishments of last 3 years, including U-235 signature search, comparison of different photon sources, and examination of NRF measurements using monochromatic photon source.

  14. Calibration of fluorescence resonance energy transfer in microscopy

    DOE Patents [OSTI]

    Youvan, Douglas C. (San Jose, CA); Silva, Christopher M. (Sunnyvale, CA); Bylina, Edward J. (San Jose, CA); Coleman, William J. (Moutain View, CA); Dilworth, Michael R. (Santa Cruz, CA); Yang, Mary M. (San Jose, CA)

    2002-09-24T23:59:59.000Z

    Imaging hardware, software, calibrants, and methods are provided to visualize and quantitate the amount of Fluorescence Resonance Energy Transfer (FRET) occurring between donor and acceptor molecules in epifluorescence microscopy. The MicroFRET system compensates for overlap among donor, acceptor, and FRET spectra using well characterized fluorescent beads as standards in conjunction with radiometrically calibrated image processing techniques. The MicroFRET system also provides precisely machined epifluorescence cubes to maintain proper image registration as the sample is illuminated at the donor and acceptor excitation wavelengths. Algorithms are described that pseudocolor the image to display pixels exhibiting radiometrically-corrected fluorescence emission from the donor (blue), the acceptor (green) and FRET (red). The method is demonstrated on samples exhibiting FRET between genetically engineered derivatives of the Green Fluorescent Protein (GFP) bound to the surface of Ni chelating beads by histidine-tags.

  15. LIGHT-INDUCED CHANGES IN THE FLUORESCENCE YIELD OF

    E-Print Network [OSTI]

    Govindjee

    given during the transient slightly hastens and heightens P. Methyl viologen, an exogenous system I fluorescence intensity is the redox state of Q, the electron acceptor in the system It reaction center (9). Fig

  16. THE HISTORY AND TECHNICAL EVOLUTION OF HIGH FREQUENCY FLUORESCENT LIGHTING

    E-Print Network [OSTI]

    Campbell, John H.

    2011-01-01T23:59:59.000Z

    Dec. 1953. "Hi-Volt-Cycle Lighting ~ Launched i n a Hi-Wide2, F e b r u a r y , 19 53. "Lighting Your Plant with Highg h Frequency Fluorescent Lighting John H. Campbell December

  17. Large core polymer optical backplanes for fluorescence detection

    E-Print Network [OSTI]

    Lee, Kevin Shao-Kwan

    2006-01-01T23:59:59.000Z

    Fluorescence based sensors are used for determining environmental parameters such as dissolved oxygen or pH in biological systems without disturbing a biological system's equilibrium. Recently, there has been a drive to ...

  18. REDUCING INCIDENTAL FLUORESCENCE IN LIVE CELL IMAGING A. Altinok 1

    E-Print Network [OSTI]

    California at Santa Barbara, University of

    , the technique is based on staining bio molecules selectively with proteins carrying fluorescence dyes of known. For instance, length measurements in Fig.1 would lack accuracy since the ends of the objects are blurry within

  19. SHORT COMMUNICATION The Chlorophyll Fluorescence Ratio F735/F700

    E-Print Network [OSTI]

    Gitelson, Anatoly

    sylvatica L.), elm (Ulmus minor 1988; Dahn et al., 1992). In green leaves, about 90% of Miller), and wild of reabsorption of the Chl fluorescence was quan- titatively estimated (Dahn et al., 1992; Gunther et al

  20. Calibration of fluorescence resonance energy transfer in microscopy

    DOE Patents [OSTI]

    Youvan, Dougalas C.; Silva, Christopher M.; Bylina, Edward J.; Coleman, William J.; Dilworth, Michael R.; Yang, Mary M.

    2003-12-09T23:59:59.000Z

    Imaging hardware, software, calibrants, and methods are provided to visualize and quantitate the amount of Fluorescence Resonance Energy Transfer (FRET) occurring between donor and acceptor molecules in epifluorescence microscopy. The MicroFRET system compensates for overlap among donor, acceptor, and FRET spectra using well characterized fluorescent beads as standards in conjunction with radiometrically calibrated image processing techniques. The MicroFRET system also provides precisely machined epifluorescence cubes to maintain proper image registration as the sample is illuminated at the donor and acceptor excitation wavelengths. Algorithms are described that pseudocolor the image to display pixels exhibiting radiometrically-corrected fluorescence emission from the donor (blue), the acceptor (green) and FRET (red). The method is demonstrated on samples exhibiting FRET between genetically engineered derivatives of the Green Fluorescent Protein (GFP) bound to the surface of Ni chelating beads by histidine-tags.

  1. Exploring the mechanome with optical tweezers and single molecule fluorescence

    E-Print Network [OSTI]

    Brau, Ricardo R. (Ricardo Rafael), 1979-

    2008-01-01T23:59:59.000Z

    The combination of optical tweezers and single molecule fluorescence into an instrument capable of making combined, coincident measurements adds an observable dimension that allows for the examination of the localized ...

  2. Protein subcellular localization assays using split fluorescent proteins

    DOE Patents [OSTI]

    Waldo, Geoffrey S. (Santa Fe, NM); Cabantous, Stephanie (Los Alamos, NM)

    2009-09-08T23:59:59.000Z

    The invention provides protein subcellular localization assays using split fluorescent protein systems. The assays are conducted in living cells, do not require fixation and washing steps inherent in existing immunostaining and related techniques, and permit rapid, non-invasive, direct visualization of protein localization in living cells. The split fluorescent protein systems used in the practice of the invention generally comprise two or more self-complementing fragments of a fluorescent protein, such as GFP, wherein one or more of the fragments correspond to one or more beta-strand microdomains and are used to "tag" proteins of interest, and a complementary "assay" fragment of the fluorescent protein. Either or both of the fragments may be functionalized with a subcellular targeting sequence enabling it to be expressed in or directed to a particular subcellular compartment (i.e., the nucleus).

  3. Comparative Study Between Air-Cooled and Water-Cooled Condensers of the Air-Conditioning Systems 

    E-Print Network [OSTI]

    Maheshwari, G. P.; Mulla Ali, A. A.

    2004-01-01T23:59:59.000Z

    The weather in Kuwait is very dry where the dry-bulb temperature exceeds the wet-bulb temperature more than 20oC in most of the summer months. Thus, the air-conditioning (A/C) system with the water-cooled (WC) condensers is expected to perform more...

  4. Comparative Study Between Air-Cooled and Water-Cooled Condensers of the Air-Conditioning Systems

    E-Print Network [OSTI]

    Maheshwari, G. P.; Mulla Ali, A. A.

    2004-01-01T23:59:59.000Z

    The weather in Kuwait is very dry where the dry-bulb temperature exceeds the wet-bulb temperature more than 20oC in most of the summer months. Thus, the air-conditioning (A/C) system with the water-cooled (WC) condensers is expected to perform more...

  5. An Enhanced Monomeric Blue Fluorescent Protein with the High Chemical Stability of the Chromophore

    E-Print Network [OSTI]

    Verkhusha, Vladislav V.

    An Enhanced Monomeric Blue Fluorescent Protein with the High Chemical Stability of the Chromophore Commonly used monomeric blue fluorescent proteins suffer from moderate brightness. The brightest of them, m transition from a blue fluorescent state with absorbance at 401 nm to a non-fluorescent state with absorbance

  6. Development of laser excited atomic fluorescence and ionization methods

    SciTech Connect (OSTI)

    Winefordner, J.D.

    1991-01-01T23:59:59.000Z

    Progress report: May 1, 1988 to December 31, 1991. The research supported by DE-FG05-88ER13881 during the past (nearly) 3 years can be divided into the following four categories: (1) theoretical considerations of the ultimate detection powers of laser fluorescence and laser ionization methods; (2) experimental evaluation of laser excited atomic fluorescence; (3) fundamental studies of atomic and molecular parameters in flames and plasmas; (4) other studies.

  7. LucY: A Versatile New Fluorescent Reporter Protein

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Auldridge, Michele E.; Cao, Hongnan; Sen, Saurabh; Franz, Laura P.; Bingman, Craig A.; Yennamalli, Ragothaman M.; Phillips, George N.; Mead, David; Steinmetz, Eric J.; Michnick, Stephen W.

    2015-04-23T23:59:59.000Z

    We report on the discovery, isolation, and use of a novel yellow fluorescent protein. Lucigen Yellow (LucY) binds one FAD molecule within its core, thus shielding it from water and maintaining its structure so that fluorescence is 10-fold higher than freely soluble FAD. LucY displays excitation and emission spectra characteristic of FAD, with 3 excitation peaks at 276nm, 377nm, and 460nm and a single emission peak at 530nm. These excitation and emission maxima provide the large Stokes shift beneficial to fluorescence experimentation. LucY belongs to the MurB family of UDP-N-acetylenolpyruvylglucosamine reductases. The high resolution crystal structure shows that in contrastmore »to other structurally resolved MurB enzymes, LucY does not contain a potentially quenching aromatic residue near the FAD isoalloxazine ring, which may explain its increased fluorescence over related proteins. Using E. coli as a system in which to develop LucY as a reporter, we show that it is amenable to circular permutation and use as a reporter of protein-protein interaction. Fragmentation between its distinct domains renders LucY non-fluorescent, but fluorescence can be partially restored by fusion of the fragments to interacting protein domains. Thus, LucY may find application in Protein-fragment Complementation Assays for evaluating protein-protein interactions.« less

  8. Application of fluorescence microscopy to coal-derived resid characterization

    SciTech Connect (OSTI)

    Rathbone, R.F.; Hower, J.C.; Derbyshire, F.J.

    1991-01-01T23:59:59.000Z

    This study evaluates the usefulness of a fluorescence microscopy methodology to analyze coal-derived resids and interpret the data in the light of liquefaction processing conditions, process response, the inferred resid reactivity, and in relation to results of other analytical data. The fluorescence technique utilized has been widely applied to coal and kerogen characterization, albeit with some modifications, but is novel in its application to the characterization of coal liquids. Fluorescence is the emission of light energy which occurs when electrons, having been excited to a higher energy orbital, return to their lower energy ground state. The majority of organic molecules that fluoresce are those with conjugated double bonds (chromophores), such as aromatics, characterized by pi-electrons less strongly bound within the molecule than sigma electrons, that can be excited to anti-bonding pi-orbitals. Increasing the extent of pi-bond conjugation (i.e. larger molecular size) generally imparts a shift in absorption and emission spectra to longer wavelengths. Resid fluorescence largely depends on the concentration and degree of conjugation of aromatic chromophores in the high molecular weight liquids, possibly with ancillary effects from oxygen functionalities. In this context, fluorescence analysis of liquefaction resids can potentially evaluate process performance, since direct liquefaction processes endeavor to break down the macromolecular structure of coal, and reduce the molecular weight of polycondensed aromatics through hydrogenation, the opening of ring structures, and heteroatom removal.

  9. Application of fluorescence microscopy to coal-derived resid characterization

    SciTech Connect (OSTI)

    Rathbone, R.F.; Hower, J.C.; Derbyshire, F.J.

    1991-12-31T23:59:59.000Z

    This study evaluates the usefulness of a fluorescence microscopy methodology to analyze coal-derived resids and interpret the data in the light of liquefaction processing conditions, process response, the inferred resid reactivity, and in relation to results of other analytical data. The fluorescence technique utilized has been widely applied to coal and kerogen characterization, albeit with some modifications, but is novel in its application to the characterization of coal liquids. Fluorescence is the emission of light energy which occurs when electrons, having been excited to a higher energy orbital, return to their lower energy ground state. The majority of organic molecules that fluoresce are those with conjugated double bonds (chromophores), such as aromatics, characterized by pi-electrons less strongly bound within the molecule than sigma electrons, that can be excited to anti-bonding pi-orbitals. Increasing the extent of pi-bond conjugation (i.e. larger molecular size) generally imparts a shift in absorption and emission spectra to longer wavelengths. Resid fluorescence largely depends on the concentration and degree of conjugation of aromatic chromophores in the high molecular weight liquids, possibly with ancillary effects from oxygen functionalities. In this context, fluorescence analysis of liquefaction resids can potentially evaluate process performance, since direct liquefaction processes endeavor to break down the macromolecular structure of coal, and reduce the molecular weight of polycondensed aromatics through hydrogenation, the opening of ring structures, and heteroatom removal.

  10. Rigidifying Fluorescent Linkers by Metal–Organic Framework Formation for Fluorescence Blue Shift and Quantum Yield Enhancement

    SciTech Connect (OSTI)

    Wei, Zhangwen; Gu, Zhi-Yuan; Arvapally, Ravi K.; Chen, Ying-Pin; Ivy, Joshua F.; Yakovenko, Andrey A.; Feng, Dawei; Omary, Mohammad A.; Zhou, Hong-Cai [UNT

    2014-06-11T23:59:59.000Z

    We demonstrate that rigidifying the structure of fluorescent linkers by structurally constraining them in metal–organic frameworks (MOFs) to control their conformation effectively tunes the fluorescence energy and enhances the quantum yield. Thus, a new tetraphenylethylene-based zirconium MOF exhibits a deep-blue fluorescent emission at 470 nm with a unity quantum yield (99.9 ± 0.5%) under Ar, representing ca. 3600 cm?¹ blue shift and doubled radiative decay efficiency vs the linker precursor. An anomalous increase in the fluorescence lifetime and relative intensity takes place upon heating the solid MOF from cryogenic to ambient temperatures. The origin of these unusual photoluminescence properties is attributed to twisted linker conformation, intramolecular hindrance, and framework rigidity.

  11. Fluorescence-dip infrared spectroscopy of tropolone and tropolone-OD Rex K. Frost, Fredrick C. Hagemeister, Caleb A. Arrington, and Timothy S. Zwiera)

    E-Print Network [OSTI]

    Zwier, Timothy S.

    Fluorescence-dip infrared spectroscopy of tropolone and tropolone-OD Rex K. Frost, Fredrick COH and its singly deuterated isotopomer TrOD in the O­H and C­H stretch regions. The ability of the method in the present study. The spectra of TrOH are compared with those from deuterated tropolone TrOD to assign

  12. Efficient defrosting of an inclined flat surface Subrata Roy *, Haribalan Kumar, Richard Anderson

    E-Print Network [OSTI]

    Roy, Subrata

    and compared results with thermography and hot bulb type of measurements [12]. However, a correlation

  13. Cyanine-based probe\\tag-peptide pair fluorescence protein imaging and fluorescence protein imaging methods

    DOE Patents [OSTI]

    Mayer-Cumblidge, M. Uljana; Cao, Haishi

    2013-01-15T23:59:59.000Z

    A molecular probe comprises two arsenic atoms and at least one cyanine based moiety. A method of producing a molecular probe includes providing a molecule having a first formula, treating the molecule with HgOAc, and subsequently transmetallizing with AsCl.sub.3. The As is liganded to ethanedithiol to produce a probe having a second formula. A method of labeling a peptide includes providing a peptide comprising a tag sequence and contacting the peptide with a biarsenical molecular probe. A complex is formed comprising the tag sequence and the molecular probe. A method of studying a peptide includes providing a mixture containing a peptide comprising a peptide tag sequence, adding a biarsenical probe to the mixture, and monitoring the fluorescence of the mixture.

  14. Ferroelectric optical image comparator

    DOE Patents [OSTI]

    Butler, M.A.; Land, C.E.; Martin, S.J.; Pfeifer, K.B.

    1993-11-30T23:59:59.000Z

    A ferroelectric optical image comparator has a lead lanthanum zirconate titanate thin-film device which is constructed with a semi-transparent or transparent conductive first electrode on one side of the thin film, a conductive metal second electrode on the other side of the thin film, and the second electrode is in contact with a nonconducting substrate. A photoinduced current in the device represents the dot product between a stored image and an image projected onto the first electrode. One-dimensional autocorrelations are performed by measuring this current while displacing the projected image. 7 figures.

  15. Laser-induced differential normalized fluorescence method for cancer diagnosis

    DOE Patents [OSTI]

    Vo-Dinh, T.; Panjehpour, M.; Overholt, B.F.

    1996-12-03T23:59:59.000Z

    An apparatus and method for cancer diagnosis are disclosed. The diagnostic method includes the steps of irradiating a tissue sample with monochromatic excitation light, producing a laser-induced fluorescence spectrum from emission radiation generated by interaction of the excitation light with the tissue sample, and dividing the intensity at each wavelength of the laser-induced fluorescence spectrum by the integrated area under the laser-induced fluorescence spectrum to produce a normalized spectrum. A mathematical difference between the normalized spectrum and an average value of a reference set of normalized spectra which correspond to normal tissues is calculated, which provides for amplifying small changes in weak signals from malignant tissues for improved analysis. The calculated differential normalized spectrum is correlated to a specific condition of a tissue sample. 5 figs.

  16. Laser-induced differential normalized fluorescence method for cancer diagnosis

    DOE Patents [OSTI]

    Vo-Dinh, Tuan (Knoxville, TN); Panjehpour, Masoud (Knoxville, TN); Overholt, Bergein F. (Knoxville, TN)

    1996-01-01T23:59:59.000Z

    An apparatus and method for cancer diagnosis are disclosed. The diagnostic method includes the steps of irradiating a tissue sample with monochromatic excitation light, producing a laser-induced fluorescence spectrum from emission radiation generated by interaction of the excitation light with the tissue sample, and dividing the intensity at each wavelength of the laser-induced fluorescence spectrum by the integrated area under the laser-induced fluorescence spectrum to produce a normalized spectrum. A mathematical difference between the normalized spectrum and an average value of a reference set of normalized spectra which correspond to normal tissues is calculated, which provides for amplifying small changes in weak signals from malignant tissues for improved analysis. The calculated differential normalized spectrum is correlated to a specific condition of a tissue sample.

  17. Fluorescent single walled nanotube/silica composite materials

    SciTech Connect (OSTI)

    Dattelbaum, Andrew M.; Gupta, Gautam; Duque, Juan G.; Doorn, Stephen K.; Hamilton, Christopher E.; DeFriend Obrey, Kimberly A.

    2013-03-12T23:59:59.000Z

    Fluorescent composites of surfactant-wrapped single-walled carbon nanotubes (SWNTs) were prepared by exposing suspensions of surfactant-wrapped carbon nanotubes to tetramethylorthosilicate (TMOS) vapor. Sodium deoxycholate (DOC) and sodium dodecylsulphate (SDS) were the surfactants. No loss in emission intensity was observed when the suspension of DOC-wrapped SWNTs were exposed to the TMOS vapors, but about a 50% decrease in the emission signal was observed from the SDS-wrapped SWNTs nanotubes. The decrease in emission was minimal by buffering the SDS/SWNT suspension prior to forming the composite. Fluorescent xerogels were prepared by adding glycerol to the SWNT suspensions prior to TMOS vapor exposure, followed by drying the gels. Fluorescent aerogels were prepared by replacing water in the gels with methanol and then exposing them to supercritical fluid drying conditions. The aerogels can be used for gas sensing.

  18. Photosynthesis: Research for Food, Fuel and Future--15th International Conference on Photosynthesis518 FLIM (Fluorescence Lifetime Imaging Microscopy) of Avocado Leaves during Slow

    E-Print Network [OSTI]

    Govindjee

    518 FLIM (Fluorescence Lifetime Imaging Microscopy) of Avocado Leaves during Slow Fluorescence avocado leaves (Persea americana Mill.) during the slow part of chlorophyll (Chl) a fluorescence transient

  19. Nuclear Resonance Fluorescence for Safeguards Applications

    SciTech Connect (OSTI)

    Ludewigt, Bernhard A; Quiter, Brian J; Ambers, Scott D

    2011-02-04T23:59:59.000Z

    In nuclear resonance fluorescence (NRF) measurements, resonances are excited by an external photon beam leading to the emission of {gamma} rays with specific energies that are characteristic of the emitting isotope. The promise of NRF as a non-destructive analysis technique (NDA) in safeguards applications lies in its potential to directly quantify a specific isotope in an assay target without the need for unfolding the combined responses of several fissile isotopes as often required by other NDA methods. The use of NRF for detection of sensitive nuclear materials and other contraband has been researched in the past. In the safeguards applications considered here one has to go beyond mere detection and precisely quantify the isotopic content, a challenge that is discussed throughout this report. Basic NRF measurement methods, instrumentation, and the analytical calculation of NRF signal strengths are described in Section 2. Well understood modeling and simulation tools are needed for assessing the potential of NRF for safeguards and for designing measurement systems. All our simulations were performed with the radiation transport code MCNPX, a code that is widely used in the safeguards community. Our initial studies showed that MCNPX grossly underestimated the elastically scattered background at backwards angles due to an incorrect treatment of Rayleigh scattering. While new, corrected calculations based on ENDF form factors showed much better agreement with experimental data for the elastic scattering of photons on an uranium target, the elastic backscatter is still not rigorously treated. Photonuclear scattering processes (nuclear Thomson, Delbruck and Giant Dipole Resonance scattering), which are expected to play an important role at higher energies, are not yet included. These missing elastic scattering contributions were studied and their importance evaluated evaluated against data found in the literature as discussed in Section 3. A transmission experiment was performed in September 2009 to test and demonstrate the applicability of the method to the quantitative measurement of an isotope of interest embedded in a thick target. The experiment, data analysis, and results are described in Section 4. The broad goal of our NRF studies is to assess the potential of the technique in safeguards applications. Three examples are analyzed in Section 5: the isotopic assay of spent nuclear fuel (SNF), the measurement of {sup 235}U enrichment in UF{sub 6} cylinders, and the determination of {sup 239}Pu in mixed oxide (MOX) fuel. The study of NRF for the assay of SNF assemblies was supported by the Next Generation Safeguards Initiative (NGSI) of the U.S. Department of Energy as part of a large multi-lab/university effort to quantify the plutonium (Pu) mass in spent nuclear fuel assemblies and to detect the diversion of pins with non-destructive assay (NDA) methods. NRF is one of 14 NDA techniques being researched. The methodology for performing and analyzing quantitative NRF measurements was developed for determining Pu mass in SNF and is extensively discussed in this report. The same methodology was applied to the assessment of NRF for the measurement of {sup 235}U enrichment and the determination of {sup 239}Pu in MOX fuel. The analysis centers on determining suitable NRF measurement methods, measurement capabilities that could be realized with currently available instrumentation, and photon source and detector requirements for achieving useful NDA capabilities.

  20. FOREST SERVICE Forest andRangeU. S.DEPARTMENT OF AGRICULTURE P. 0.BOX 245, BERKELEY, CALIFORNIA 94701 Experime

    E-Print Network [OSTI]

    Standiford, Richard B.

    , Riverside, California Dry bulb temperature range Dry bulb temperature control tolerance Dry bulb temperature. to 120' F. 1loF. Min. 40° F./hr. -23' F. to +20° F. @ 25' F. dry bulb, and 39' F. to 90' F. @ 120° F. dry bulb with comparable limits between these dry bulb temperatures. rt l0F. Min. lo0F./hr. 3.5 to 100ft

  1. An Event Reconstruction Method for the Telescope Array Fluorescence Detectors

    SciTech Connect (OSTI)

    Fujii, T.; Ogio, S.; Yamazaki, K. [Graduate Schiool of Science, Osaka City University, Sumiyoshi, Osaka 558-8585 (Japan); Fukushima, M.; Ikeda, D.; Sagawa, H.; Takahashi, Y.; Tameda, Y. [Institute of Cosmic Ray Research, University of Tokyo, Kashiwa, Chiba 277-8582 (Japan); Hayashi, K.; Ishimori, R.; Kobayashi, Y.; Tokuno, H.; Tsunesada, Y. [Graduate School of Science and Engineering, Tokyo Institute of Technology, Meguro, Tokyo 152-8551 (Japan); Honda, K.; Tomida, T. [Graduate School of Medicine and Engineering, University of Yamanashi, Kofu, Yamanashi 400-8511 (Japan); Udo, S. [Faculty of Engineering, Kanagawa University, Yokohama, Kanagawa 221-8686 (Japan)

    2011-09-22T23:59:59.000Z

    We measure arrival directions, energies and mass composition of ultra-high energy cosmic rays with air fluorescence detector telescopes. The longitudinal profile of the cosmic ray induced extensive air shower cascade is imaged on focal plane of the telescope camera. Here, we show an event reconstruction method to obtain the primary information from data collected by the Telescope Array Fluorescence Detectors. In particular, we report on an ''Inverse Monte Carlo (IMC)'' method in which the reconstruction process searches for an optimum solution via repeated Monte Carlo simulations including characteristics of all detectors, atmospheric conditions, photon emission and scattering processes.

  2. Longitudinal Shower Profile Reconstruction from Fluorescence and Cherenkov Light

    E-Print Network [OSTI]

    M. Unger; R. Engel; F. Schüssler; R. Ulrich

    2007-06-11T23:59:59.000Z

    Traditionally, longitudinal shower profiles are reconstructed in fluorescence light experiments by treating the Cherenkov light contribution as background. Here we will argue that, due to universality of the energy spectra of electrons and positrons, both fluorescence and Cherenkov light can be used simultaneously as signal to infer the longitudinal shower development. We present a new profile reconstruction method that is based on the analytic least-square solution for the estimation of the shower profile from the observed light signal and discuss the extrapolation of the profile with a Gaisser-Hillas function.

  3. A versatile detector for total fluorescence and electron yield experiments

    SciTech Connect (OSTI)

    Thielemann, N. [Institute for Methods and Instrumentation for Synchrotron Radiation Research, Helmholtz-Zentrum Berlin fuer Materialien und Energie GmbH, Albert-Einstein-Str. 15, 12489 Berlin (Germany); Institut fuer Physik, Humboldt-Universitaet zu Berlin, Newtonstrasse 15, 12489 Berlin (Germany); Hoffmann, P. [Institute for Methods and Instrumentation for Synchrotron Radiation Research, Helmholtz-Zentrum Berlin fuer Materialien und Energie GmbH, Albert-Einstein-Str. 15, 12489 Berlin (Germany); Foehlisch, A. [Institute for Methods and Instrumentation for Synchrotron Radiation Research, Helmholtz-Zentrum Berlin fuer Materialien und Energie GmbH, Albert-Einstein-Str. 15, 12489 Berlin (Germany); Institut fuer Physik und Astronomie, Universitaet Potsdam, Karl-Liebknecht-Strasse 24-25, 14476 Potsdam (Germany)

    2012-09-15T23:59:59.000Z

    The combination of a non-coated silicon photodiode with electron repelling meshes makes a versatile detector for total fluorescence yield and electron yield techniques highly suitable for x-ray absorption spectroscopy. In particular, a copper mesh with a bias voltage allows to suppress or transmit the electron yield signal. The performance of this detection scheme has been characterized by near edge x-ray absorption fine structure studies of thermal oxidized silicon and sapphire. The results show that the new detector probes both electron yield and for a bias voltage exceeding the maximum photon energy the total fluorescence yield.

  4. Compact Fluorescent Plug-In Ballast-in-a-Socket

    SciTech Connect (OSTI)

    Rebecca Voelker

    2001-12-21T23:59:59.000Z

    The primary goal of this program was to develop a ballast system for plug-in CFLs (compact fluorescent lamps) that will directly replace standard metal shell, medium base incandescent lampholders (such as Levition No. 6098) for use with portable lamp fixtures, such as floor, table and desk lamps. A secondary goal was to identify a plug-in CFL that is optimized for use with this ballast. This Plug-in CFL Ballastin-a-Socket system will allow fixture manufacturers to easily manufacture CFL-based high-efficacy portable fixtures that provide residential and commercial consumers with attractive, cost-effective, and energy-efficient fixtures for use wherever portable incandescent fixtures are used today. The advantages of this proposed system over existing CFL solutions are that the fixtures can only be used with high-efficacy CFLs, and they will be more attractive and will have lower life-cycle costs than screw-in or adapter-based CFL retrofit solutions. These features should greatly increase the penetration of CFL's into the North American market. Our work has shown that using integrated circuits it is quite feasible to produce a lamp-fixture ballast of a size comparable to the current Edison-screw 3-way incandescent fixtures. As for price points for BIAS-based fixtures, end-users polled by the Lighting Research Institute at RPI indicated that they would pay as much as an additional $10 for a lamp containing such a ballast. The ballast has been optimized to run with a 26 W amalgam triple biax lamp in the base-down position, yet can accept non-amalgam versions of the lamp. With a few part alterations, the ballast can be produced to support 32 W lamps as well. The ballast uses GE's existing L-Comp[1] power topology in the circuit so that the integrated circuit design would be a design that could possibly be used by other CFL and EFL products with minor modifications. This gives added value by reducing cost and size of not only the BIAS, but also possibly other integral CFL and future dimmable integral and plug-in versions of the EFL products.

  5. Feasibility of x ray fluorescence for spent fuel safeguards

    SciTech Connect (OSTI)

    Freeman, Corey Ross [Los Alamos National Laboratory; Mozin, Vladimir [Los Alamos National Laboratory; Tobin, Stephen J [Los Alamos National Laboratory; Fensin, Michael L [Los Alamos National Laboratory; White, Julia M [Los Alamos National Laboratory; Croft, Stephen [Los Alamos National Laboratory; Stafford, Alissa [TAMU; Charlton, William [TAMU

    2010-01-01T23:59:59.000Z

    Quantifying the Pu content in spent nuclear fuel is necessary for many reasons, in particular to verify that diversion or other illicit activities have not occurred. Therefore, safeguarding the world's nuclear fuel is paramount to responsible nuclear regulation and public acceptance, but achieving this goal presents many difficulties from both a technical and economic perspective. The Next Generation Safeguards Initiative (NGSI) of NA-24 is funding a large collaborative effort between multiple laboratories and universities to improve spent nuclear fuel safeguards methods and equipment. This effort involves the current work of modeling several different nondestructive assay (NDA) techniques. Several are being researched, because no single NDA technique, in isolation, has the potential to properly characterize fuel assemblies and offer a robust safeguards measure. The insights gained from this research, will be used to down-select from the original set a few of the most promising techniques that complement each other. The goal is to integrate the selected instruments to create an accurate measurement system for fuel verification that is also robust enough to detect diversions. These instruments will be fabricated and tested under realistic conditions. This work examines one of the NDA techniques; the feasibility of using x ray emission peaks from Pu and U to gather information about their relative quantities in the spent fuel. X Ray Fluorescence (XRF), is unique compared to the investigated techniques in that it is the only one able to give the elemental ratio of Pu to U, allowing the possibility of a Pu gram quantity for the assembly to be calculated. XRF also presents many challenges, mainly its low penetration, since the low energy x rays of interest are effectively shielded by the first few millimeters of a fuel pin. This paper will explore the results of Monte Carlo N-Particle eXtended (MCNPX) transport code calculations of spent fuel x ray peaks. The MCNPX simulations will be benchmarked against measurements taken at Oak Ridge. Analysis of the feasibility of XRFs role in spent nuclear fuel safeguards efforts, particularly in the context of the overall NGSI effort will be discussed.

  6. Fluorescent Dye Encapsulated ZnO Particles with Cell-specific...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    the visible fluorescence emission of the dye or UV fluorescence emission of ZnO, and anti-cancerantibacterial treatments using the selective toxicity of the nanoscale ZnO outer...

  7. Classification with Artificial Neural Networks and Support Vector Machines: application to oil fluorescence spectra

    E-Print Network [OSTI]

    Oldenburg, Carl von Ossietzky Universität

    methods to examine crude oils, heavy refined oils, and sludge oils: the channels relationships method (CRMClassification with Artificial Neural Networks and Support Vector Machines: application to oil, and Oil fluorescence ABSTRACT: This paper reports on oil classification with fluorescence spectroscopy

  8. Detection of Ethylene Gas by Fluorescence Turn-On of a Conjugated Polymer

    E-Print Network [OSTI]

    Swager, Timothy Manning

    Ripe fruits: The fluorescence of a conjugated polymer is quenched by the presence of copper(I) moieties. Upon exposure to ethylene gas the copper complexes bind to ethylene and no longer quench the polymer fluorescence ...

  9. Extending the utility of enzymes for site-specific targeting of fluorescent probes

    E-Print Network [OSTI]

    Liu, Daniel S. (Daniel Shao-Chen)

    2014-01-01T23:59:59.000Z

    Genetically encodable fluorescence reporters such as the green fluorescent protein (GFP) are useful for studying protein expression, localization, and dynamics in a variety of biological systems. GFP and its related variants, ...

  10. Surfactant Diffusion into Lysozyme Crystal Matrices Investigated by Quantitative Fluorescence Microscopy

    E-Print Network [OSTI]

    Velev, Orlin D.

    the infusion of lysozyme crystals with pyrene-based fluorescent surfactants by quantitative fluorescence × 10-10 to 30 × 10-10 cm2 /s, depending on the type and size of the surfactant. The slow infusion

  11. Optical gating of perylene bisimide fluorescence using dithienylcyclopentene photochromic switches

    SciTech Connect (OSTI)

    Pärs, Martti; Köhler, Jürgen, E-mail: juergen.koehler@uni-bayreuth.de [Experimental Physics IV, University of Bayreuth, 95440 Bayreuth (Germany)] [Experimental Physics IV, University of Bayreuth, 95440 Bayreuth (Germany); Gräf, Katja; Bauer, Peter; Thelakkat, Mukundan [Applied Functional Polymers, University of Bayreuth, 95440 Bayreuth (Germany)] [Applied Functional Polymers, University of Bayreuth, 95440 Bayreuth (Germany)

    2013-11-25T23:59:59.000Z

    The emission of millions of fluorescence photons from a chromophore is controlled by the absorption of a few tens of photons in a photochromic molecule. The parameters that determine the efficiency of this process are investigated, providing insights for the development of an all-optical gate.

  12. EMERGING TECHNOLOGIES Two-photon excitation chlorophyll fluorescence lifetime imaging

    E-Print Network [OSTI]

    Qu, Jianan

    at higher Cd concentration was clearly identified. The decay of chlorophyll fluorescence extracted from) toxicity Abbreviations Cd Cadmium PS Photosystem LHCII Light-harvesting complex II PAM Pulse and Computer Engineering, Hong Kong University of Science and Technology (HKUST), Clear Water Bay, Kowloon

  13. Fluorescent lamp with static magnetic field generating means

    DOE Patents [OSTI]

    Moskowitz, P.E.; Maya, J.

    1987-09-08T23:59:59.000Z

    A fluorescent lamp wherein magnetic field generating means (e.g., permanent magnets) are utilized to generate a static magnetic field across the respective electrode structures of the lamp such that maximum field strength is located at the electrode's filament. An increase in efficacy during operation has been observed. 2 figs.

  14. Diffusion of Polymer-Coated Nanoparticles Studied by Fluorescence Correlation

    E-Print Network [OSTI]

    Granick, Steve

    Diffusion of Polymer-Coated Nanoparticles Studied by Fluorescence Correlation Spectroscopy Jiang of Brownian motion of polystyrene nanoparticles is controlled by tuning their hydro- dynamic radius through coefficient of the particles. This study has initiated our further studies on the interaction of nanoparticles

  15. Aqueous Ferrofluid of Magnetite Nanoparticles: Fluorescence Labeling and Magnetophoretic Control

    E-Print Network [OSTI]

    Swihart, Mark T.

    functionalization of magnetite nanoparticles using citric acid to produce a stable aqueous dispersionAqueous Ferrofluid of Magnetite Nanoparticles: Fluorescence Labeling and Magnetophoretic ControlVed: October 8, 2004 A method is presented for the preparation of a biocompatible ferrofluid containing dye-functionalized

  16. Classification of Protein Localization Patterns Obtained via Fluorescence Light Microscopy

    E-Print Network [OSTI]

    Gordon, Geoffrey J.

    of the microscope. They should also be in- sensitive to the wide variability in cell morphology that is present in fluores- cence light microscope images of mammalian cells. Such images are generated on a regular basis by labeling one or more subcellular structures with fluorescent dyes and then collecting images

  17. Convection venting lensed reflector-type compact fluorescent lamp system

    DOE Patents [OSTI]

    Pelton, B.A.; Siminovitch, M.

    1997-07-29T23:59:59.000Z

    Disclosed herein is a fluorescent lamp housing assembly capable of providing convection cooling to the lamp and the ballast. The lens of the present invention includes two distinct portions, a central portion and an apertured portion. The housing assembly further includes apertures so that air mass is able to freely move up through the assembly and out ventilation apertures. 12 figs.

  18. Convection venting lensed reflector-type compact fluorescent lamp system

    DOE Patents [OSTI]

    Pelton, Bruce A. (825 Manor Rd., El Sobrante, CA 94803); Siminovitch, Michael (829 Manor Rd., El Sobrante, CA 94803)

    1997-01-01T23:59:59.000Z

    Disclosed herein is a fluorescent lamp housing assembly capable of providing convection cooling to the lamp and the ballast. The lens of the present invention includes two distinct portions, a central portion and an apertured portion. The housing assembly further includes apertures so that air mass is able to freely move up through the assembly and out ventilation apertures.

  19. Cellubrevin-targeted Fluorescence Uncovers Heterogeneity in the Recycling Endosomes*

    E-Print Network [OSTI]

    Machen, Terry E.

    Cellubrevin-targeted Fluorescence Uncovers Heterogeneity in the Recycling Endosomes* (Received, University of California, Berkeley, California 94720-3200 The pH and trafficking of recycling endosomes have-enriched recycling endosomes (pHCb) and FITC-transferrin to measure the pH of transferrin- enriched recycling

  20. Enhanced Archaerhodopsin Fluorescent Protein Voltage Yiyang Gong1,2*

    E-Print Network [OSTI]

    Ferrari, Silvia

    Enhanced Archaerhodopsin Fluorescent Protein Voltage Indicators Yiyang Gong1,2* , Jin Zhong Li1 by nearly three-fold in comparison to Arch-D95N. Citation: Gong Y, Li JZ, Schnitzer MJ (2013) Enhanced; Published June 19, 2013 Copyright: © 2013 Gong et al. This is an open-access article distributed under

  1. Identication of Major Water-Soluble Fluorescent Components of Some

    E-Print Network [OSTI]

    Myrick, Michael Lenn

    limits for PAHs in water are in the parts-per-billion to parts-per- trillion range for a varietyIdenti®cation of Major Water-Soluble Fluorescent Components of Some Petrochemicals M. GRONER, A. R the ultraviolet (UV) ¯uorescence of water after exposure to gasoline, diesel fuel and crude oil are pre- sented

  2. Automatically Identifying Scatter in Fluorescence Data using Robust Techniques.

    E-Print Network [OSTI]

    (PARAFAC) to fluorescence excitation-emission data (EEM). The scatter does not contain any relevant handle first and second order Rayleigh scatter as well as Raman scatter in various types of EEM data of such a measurement is an excitation-emission matrix (EEM). When several samples (I) are mea- sured the data can

  3. Laser fluorescence EEM probe for cone penetrometer pollution analysis

    SciTech Connect (OSTI)

    Lin, J.; Hart, S.J.; Taylor, T.A.; Kenny, J.E. [Tufts Univ., Medford, MA (United States). Dept. of Chemistry

    1995-12-31T23:59:59.000Z

    A laser-induced fluorescence (LIF) excitation-emission matrix (EEM) probe has been developed in the laboratory, and installed and tested in a cone penetrometer. The laser excitation system uses the fourth harmonic of a flashlamp-pumped Nd:YAG laser (at 266 nm) to pump a Raman shifter. Up to ten laser beams (in the wavelength region of 257 to 400 nm) from the Raman shifter are launched into optical fibers that are connected to the optical fibers of the cone penetrometer probe through standard connectors. In the probe head, the laser radiation is focused onto the outer surface of sapphire windows that are in contact with the soils. The fluorescence emission is collected by ten collection fibers that take the fluorescence to a detection system consisting of a spectrograph and a CCD detector. This probe allows real-time collection of LIF-EEMs of pollutants adsorbed on solids or dissolved in groundwater. LIF-EEMs provide a substantial amount of spectral information that can be used to determine the composition and quantity of pollutants in soils. This probe can be used to measure POL (petroleum, oil, lubricants), PAH (polycyclic aromatic hydrocarbons), and other fluorescent pollutants. The LIF-EEM instrument has been developed in the laboratory, and installed in a cone penetrometer truck for a field test at Hill Air Force Base, Utah. The experience of the test will be discussed.

  4. Optimal resolution in Fresnel incoherent correlation holographic fluorescence microscopy

    E-Print Network [OSTI]

    Rosen, Joseph

    Optimal resolution in Fresnel incoherent correlation holographic fluorescence microscopy Gary, Israel 4 rosen@ee.bgu.ac.il *gbrooker@jhu.edu Abstract: Fresnel Incoherent Correlation Holography (FINCH. Rosen and G. Brooker, "Digital spatially incoherent Fresnel holography," Opt. Lett. 32(8), 912­914 (2007

  5. Amplifying Fluorescent Polymers Direct Synthesis of an OligonucleotidePoly-

    E-Print Network [OSTI]

    Tan, Weihong

    Amplifying Fluorescent Polymers Direct Synthesis of an Oligonucleotide­Poly- (phenylene ethynylene bioprobes can be constructed with these polymers for selective target recog- nition. To achieve this objective, the polymer must be conjugated with a biomolecule such as a DNA strand, a peptide, or a protein

  6. Automated hybridization/imaging device for fluorescent multiplex DNA sequencing

    DOE Patents [OSTI]

    Weiss, R.B.; Kimball, A.W.; Gesteland, R.F.; Ferguson, F.M.; Dunn, D.M.; Di Sera, L.J.; Cherry, J.L.

    1995-11-28T23:59:59.000Z

    A method is disclosed for automated multiplex sequencing of DNA with an integrated automated imaging hybridization chamber system. This system comprises an hybridization chamber device for mounting a membrane containing size-fractionated multiplex sequencing reaction products, apparatus for fluid delivery to the chamber device, imaging apparatus for light delivery to the membrane and image recording of fluorescence emanating from the membrane while in the chamber device, and programmable controller apparatus for controlling operation of the system. The multiplex reaction products are hybridized with a probe, the enzyme (such as alkaline phosphatase) is bound to a binding moiety on the probe, and a fluorogenic substrate (such as a benzothiazole derivative) is introduced into the chamber device by the fluid delivery apparatus. The enzyme converts the fluorogenic substrate into a fluorescent product which, when illuminated in the chamber device with a beam of light from the imaging apparatus, excites fluorescence of the fluorescent product to produce a pattern of hybridization. The pattern of hybridization is imaged by a CCD camera component of the imaging apparatus to obtain a series of digital signals. These signals are converted by the controller apparatus into a string of nucleotides corresponding to the nucleotide sequence an automated sequence reader. The method and apparatus are also applicable to other membrane-based applications such as colony and plaque hybridization and Southern, Northern, and Western blots. 9 figs.

  7. Automated hybridization/imaging device for fluorescent multiplex DNA sequencing

    DOE Patents [OSTI]

    Weiss, Robert B. (Salt Lake City, UT); Kimball, Alvin W. (Salt Lake City, UT); Gesteland, Raymond F. (Salt Lake City, UT); Ferguson, F. Mark (Salt Lake City, UT); Dunn, Diane M. (West Valley City, UT); Di Sera, Leonard J. (Salt Lake City, UT); Cherry, Joshua L. (Salt Lake City, UT)

    1995-01-01T23:59:59.000Z

    A method is disclosed for automated multiplex sequencing of DNA with an integrated automated imaging hybridization chamber system. This system comprises an hybridization chamber device for mounting a membrane containing size-fractionated multiplex sequencing reaction products, apparatus for fluid delivery to the chamber device, imaging apparatus for light delivery to the membrane and image recording of fluorescence emanating from the membrane while in the chamber device, and programmable controller apparatus for controlling operation of the system. The multiplex reaction products are hybridized with a probe, then an enzyme (such as alkaline phosphatase) is bound to a binding moiety on the probe, and a fluorogenic substrate (such as a benzothiazole derivative) is introduced into the chamber device by the fluid delivery apparatus. The enzyme converts the fluorogenic substrate into a fluorescent product which, when illuminated in the chamber device with a beam of light from the imaging apparatus, excites fluorescence of the fluorescent product to produce a pattern of hybridization. The pattern of hybridization is imaged by a CCD camera component of the imaging apparatus to obtain a series of digital signals. These signals are converted by the controller apparatus into a string of nucleotides corresponding to the nucleotide sequence an automated sequence reader. The method and apparatus are also applicable to other membrane-based applications such as colony and plaque hybridization and Southern, Northern, and Western blots.

  8. REGULAR PAPER Modulation of the fluorescence yield in heliobacterial cells

    E-Print Network [OSTI]

    . They are thought to use a light-driven cyclic electron transport pathway to pump protons, and thereby phos brief flashes is inversely correlated to the variable fluorescence. Using pump-probe spectros- copy), or conversion to heat. When photosynthetic systems are operating efficiently, photochemistry dominates

  9. Assaying protein import into mitochondria using fluorescence spectroscopy

    E-Print Network [OSTI]

    Cargill, Holly Beth

    2006-08-16T23:59:59.000Z

    of the Outer Membrane (TOM) complex, and the inner mitochondrial membrane (IM) via the Translocase of the Inner Membrane 23 (TIM23) complex. A novel system was set up to examine the import of matrix-targeted preproteins into mitochondria using fluorescence...

  10. Quantitative high dynamic range beam profiling for fluorescence microscopy

    SciTech Connect (OSTI)

    Mitchell, T. J., E-mail: t.j.mitchell@dur.ac.uk; Saunter, C. D.; O’Nions, W.; Girkin, J. M.; Love, G. D. [Centre for Advanced Instrumentation and Biophysical Sciences Institute, Department of Physics, Durham University, Durham DH1 3LE (United Kingdom)

    2014-10-15T23:59:59.000Z

    Modern developmental biology relies on optically sectioning fluorescence microscope techniques to produce non-destructive in vivo images of developing specimens at high resolution in three dimensions. As optimal performance of these techniques is reliant on the three-dimensional (3D) intensity profile of the illumination employed, the ability to directly record and analyze these profiles is of great use to the fluorescence microscopist or instrument builder. Though excitation beam profiles can be measured indirectly using a sample of fluorescent beads and recording the emission along the microscope detection path, we demonstrate an alternative approach where a miniature camera sensor is used directly within the illumination beam. Measurements taken using our approach are solely concerned with the illumination optics as the detection optics are not involved. We present a miniature beam profiling device and high dynamic range flux reconstruction algorithm that together are capable of accurately reproducing quantitative 3D flux maps over a large focal volume. Performance of this beam profiling system is verified within an optical test bench and demonstrated for fluorescence microscopy by profiling the low NA illumination beam of a single plane illumination microscope. The generality and success of this approach showcases a widely flexible beam amplitude diagnostic tool for use within the life sciences.

  11. Detection of counterfeit U.S. paper money using intrinsic fluorescence lifetime

    E-Print Network [OSTI]

    Levene, Michael J.

    -photon spectral and lifetime fluorescence microscopy," Appl. Opt. 43(27), 5173­5182 (2004). #118121 - $15.00 USD

  12. Caged Molecular Fluorescence Velocimetry to measure meso-to micro-scale thermal flow fields

    E-Print Network [OSTI]

    Park, Jaesung

    2000-01-01T23:59:59.000Z

    (CPL). The system utilizes a microscope objective lens, caged molecular fluorescence probes, Nd:YAG laser for UV light source, Ar-ion laser for 488 nm fluorescence pumping, and a color CCD camera to record a series of fluorescent images. Caged...

  13. 428 BIOCHIMICAET BIOPHYSICAACTA pH CONTROL OF THE CHLOROPHYLL a FLUORESCENCE IN ALGAE

    E-Print Network [OSTI]

    Govindjee

    428 BIOCHIMICAET BIOPHYSICAACTA BBA 46126 pH CONTROL OF THE CHLOROPHYLL a FLUORESCENCE IN ALGAE on the "slow" (min) time course of Chlorophyll a fluorescence yield in the green alga Chlorella pyrenoidosa and in the blue-green alga Anacystis nidulans. In Chlorella, the decay of fluorescence yield, in the I- to 5-rain

  14. Fluorescence Behavioral Imaging (FBI) Tracks Identity in Heterogeneous Groups of Drosophila

    E-Print Network [OSTI]

    Floreano, Dario

    Fluorescence Behavioral Imaging (FBI) Tracks Identity in Heterogeneous Groups of Drosophila Pavan describe Fluorescence Behavioral Imaging (FBI), a toolkit that uses transgenic fluorescence to discriminate-source software for automated, high-accuracy determination of genetic identity. Using FBI, we measure courtship

  15. Fluorescence quenching of water-soluble porphyrins. A novel fluorescence quenching of anionic porphyrin by anionic anthraquinone

    SciTech Connect (OSTI)

    Kano, K.; Sato, T.; Yamada, S.; Ogawa, T.

    1983-02-17T23:59:59.000Z

    The fluorescence quenching of 5-phenyl-10,15,20-tris(p-sulfonatophenyl)porphine (TPPS/sup 3 -/) and 5,10,15,20-tetrakis(4-N-methylpyridyl)porphine (TMPyP/sup 4 +/) has been studied in water (pH 8.0) by using 9,10-anthra-quinone-2,6-disulfonate (AQDS/sup 2 -/) and methyl viologen (MV/sup 2 +/) as quenchers. While electrostatic repulsion is expected, AQDS/sup 2 -/ quenched the TPPS/sup 3 -/ fluorescence more efficiently than MV/sup 2 +/. The steady-state and time-resolved fluorescence measurements indicated that static quenching took place in the TPPS/sup 3 -/-AQDS/sup 2 -/ system. Studies on the absorption spectra and the effects of ionic strength on the fluorescence quenching indicated the formation of the ground-state complex of TPPS/sup 3 -/ and AQDS/sup 2 -/. The thermodynamic parameters (..delta..H and ..delta..S) suggested that the ground-state complex was formed via van der Walls interaction.

  16. Fluorescent Probe Solubilization in the Headgroup and Core Regions of Micelles: Fluorescence Lifetime and Orientational Relaxation Measurements

    E-Print Network [OSTI]

    Fayer, Michael D.

    the microheterogeneous medium under investigation. To ascertain that the probe is located in the target environment in the hydrocarbon core region of the micelle. In this environment the chromophores have a considerably longer to be applied successfully, the fluorescent molecule must be tailored to probe the desired environment within

  17. Validation of a simple and fast method to quantify in vitro mineralization with fluorescent probes used in molecular imaging of bone

    SciTech Connect (OSTI)

    Moester, Martiene J.C. [Department of Radiology, Leiden University Medical Center (Netherlands)] [Department of Radiology, Leiden University Medical Center (Netherlands); Schoeman, Monique A.E. [Department of Orthopedic Surgery, Leiden University Medical Center (Netherlands)] [Department of Orthopedic Surgery, Leiden University Medical Center (Netherlands); Oudshoorn, Ineke B. [Department of Radiology, Leiden University Medical Center (Netherlands) [Department of Radiology, Leiden University Medical Center (Netherlands); Percuros BV, Leiden (Netherlands)] [Netherlands; Beusekom, Mara M. van [Department of Radiology, Leiden University Medical Center (Netherlands)] [Department of Radiology, Leiden University Medical Center (Netherlands); Mol, Isabel M. [Department of Radiology, Leiden University Medical Center (Netherlands) [Department of Radiology, Leiden University Medical Center (Netherlands); Percuros BV, Leiden (Netherlands)] [Netherlands; Kaijzel, Eric L.; Löwik, Clemens W.G.M. [Department of Radiology, Leiden University Medical Center (Netherlands); Rooij, Karien E. de, E-mail: k.e.de_rooij@lumc.nl [Department of Radiology, Leiden University Medical Center (Netherlands) [Department of Radiology, Leiden University Medical Center (Netherlands); Percuros BV, Leiden (Netherlands)] [Netherlands

    2014-01-03T23:59:59.000Z

    Highlights: •We validate a simple and fast method of quantification of in vitro mineralization. •Fluorescently labeled agents can detect calcium deposits in the mineralized matrix of cell cultures. •Fluorescent signals of the probes correlated with Alizarin Red S staining. -- Abstract: Alizarin Red S staining is the standard method to indicate and quantify matrix mineralization during differentiation of osteoblast cultures. KS483 cells are multipotent mouse mesenchymal progenitor cells that can differentiate into chondrocytes, adipocytes and osteoblasts and are a well-characterized model for the study of bone formation. Matrix mineralization is the last step of differentiation of bone cells and is therefore a very important outcome measure in bone research. Fluorescently labelled calcium chelating agents, e.g. BoneTag and OsteoSense, are currently used for in vivo imaging of bone. The aim of the present study was to validate these probes for fast and simple detection and quantification of in vitro matrix mineralization by KS483 cells and thus enabling high-throughput screening experiments. KS483 cells were cultured under osteogenic conditions in the presence of compounds that either stimulate or inhibit osteoblast differentiation and thereby matrix mineralization. After 21 days of differentiation, fluorescence of stained cultures was quantified with a near-infrared imager and compared to Alizarin Red S quantification. Fluorescence of both probes closely correlated to Alizarin Red S staining in both inhibiting and stimulating conditions. In addition, both compounds displayed specificity for mineralized nodules. We therefore conclude that this method of quantification of bone mineralization using fluorescent compounds is a good alternative for the Alizarin Red S staining.

  18. Comparative EPMA and -XRF methods for mapping micro-scale distribution of iodine in biocarbonates of the CallovianOxfordian

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    Comparative EPMA and -XRF methods for mapping micro-scale distribution of iodine in biocarbonates spectrometry (IR), electron microprobe (EPMA), spatially resolved synchrotron-based X-ray fluorescence (-XRF recrystallized into diagenetic calcite and celestite. EPMA and -XRF data show bioaccumulation of iodine

  19. Program Name: Simple Steps, Smart Savings Simple Steps, Smart...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and install high-quality, energy-efficient compact fluorescent lamps (CFLs), light emitting diode bulbs (LEDs), light fixtures and energy-saving showerheads. Beginning in...

  20. CX-004779: Categorical Exclusion Determination | Department of...

    Broader source: Energy.gov (indexed) [DOE]

    (i.e., install compact and linear fluorescent light bulbs and ballasts, light emitting diode emergency and exit signs lighting, occupancy sensors, andor appliances) and...

  1. U.S. Energy Information Administration (EIA) - Sector

    Annual Energy Outlook 2013 [U.S. Energy Information Administration (EIA)]

    incandescent bulbs. High-performance incandescent, compact fluorescent, and light-emitting diode (LED) lamps continue to replace low-efficacy incandescent lamps. In 2035,...

  2. U.S. Energy Information Administration (EIA) - Sector

    Annual Energy Outlook 2013 [U.S. Energy Information Administration (EIA)]

    of incandescent bulbs with more efficient compact fluorescent lighting and light-emitting diode (LED) lamps. Among electric end-use services in the residential sector,...

  3. EIA - Annual Energy Outlook 2013 Early Release

    Annual Energy Outlook 2013 [U.S. Energy Information Administration (EIA)]

    households adopt more efficient technologies, such as compact fluorescent and light-emitting diode bulbs. Commercial Commercial sector energy consumption grows from 8.6...

  4. Income Tax Deduction for Energy-Efficient Products

    Broader source: Energy.gov [DOE]

    This incentive is available for dishwashers, clothes washers, air conditioners, ceiling fans, compact fluorescent light bulbs, dehumidifiers, programmable thermostats or refrigerators that meet or...

  5. Coded Aperture Imaging for Fluorescent X-rays-Biomedical Applications

    SciTech Connect (OSTI)

    Haboub, Abdel; MacDowell, Alastair; Marchesini, Stefano; Parkinson, Dilworth

    2013-06-01T23:59:59.000Z

    Employing a coded aperture pattern in front of a charge couple device pixilated detector (CCD) allows for imaging of fluorescent x-rays (6-25KeV) being emitted from samples irradiated with x-rays. Coded apertures encode the angular direction of x-rays and allow for a large Numerical Aperture x- ray imaging system. The algorithm to develop the self-supported coded aperture pattern of the Non Two Holes Touching (NTHT) pattern was developed. The algorithms to reconstruct the x-ray image from the encoded pattern recorded were developed by means of modeling and confirmed by experiments. Samples were irradiated by monochromatic synchrotron x-ray radiation, and fluorescent x-rays from several different test metal samples were imaged through the newly developed coded aperture imaging system. By choice of the exciting energy the different metals were speciated.

  6. Fluorescence quenching of CdSe quantum dots on graphene

    SciTech Connect (OSTI)

    Guo, Xi Tao; Hua Ni, Zhen, E-mail: zhni@seu.edu.cn; Yan Nan, Hai; Hui Wang, Wen [Department of Physics and Key Laboratory of MEMS of the Ministry of Education, Southeast University, Nanjing 211189 (China)] [Department of Physics and Key Laboratory of MEMS of the Ministry of Education, Southeast University, Nanjing 211189 (China); Yan Liao, Chun [Physics Department, National Photoelectric Technology and Functional Materials and Application of Science and Technology International Cooperation Base, Northwest University, Xi'an 710069 (China)] [Physics Department, National Photoelectric Technology and Functional Materials and Application of Science and Technology International Cooperation Base, Northwest University, Xi'an 710069 (China); Zhang, Yan; Wei Zhao, Wei [Jiangsu Key Laboratory for Design and Fabrication of Micro-Nano Biomedical Instruments, School of Mechanical Engineering, Southeast University, Nanjing 211189 (China)] [Jiangsu Key Laboratory for Design and Fabrication of Micro-Nano Biomedical Instruments, School of Mechanical Engineering, Southeast University, Nanjing 211189 (China)

    2013-11-11T23:59:59.000Z

    We studied systematically the fluorescence quenching of CdSe quantum dots (QDs) on graphene and its multilayers, as well as graphene oxide (GO) and reduced graphene oxide (rGO). Raman intensity of QDs was used as a quantitatively measurement of its concentration in order to achieve a reliable quenching factor (QF). It was found that the QF of graphene (?13.1) and its multilayers is much larger than rGO (?4.4), while GO (?1.5) has the lowest quenching efficiency, which suggests that the graphitic structure is an important factor for quenching the fluorescence of QDs. It was also revealed that the QF of graphene is not strongly dependent on its thicknesses.

  7. Archaeometrical studies using X-ray fluorescence methods

    SciTech Connect (OSTI)

    Pauna, Catalina; Constantinescu, B.; Constantin, F.; Bugoi, R.; Stan, D.; Vasilescu, A. [National Institute of Nuclear Physics and Engineering, POB MG-6, 077125, Bucharest (Romania)

    2010-04-26T23:59:59.000Z

    Elemental analysis contributes to authentication (knowing the elemental composition and considering the information about the usual composition of the objects in different historical periods it can be established if the item is original or fake), provenance studies (minor and trace elements indicates ores origin and 'consequently' mines location), (relative) dating of archaeological objects (e.g. for painted items--the chemical recipes for pigments can offer information about the age of objects). The paper gives a general layout for the NIPNE Archaeometry Laboratory's applications using X-Ray Fluorescence (XRF), micro--Proton Induced X-Ray Emission (micro-PIXE), micro-Synchrotron Radiation Induced X-Ray Fluorescence (micro--SR-XRF) methods.

  8. Noise induced narrowing of metamolecule fluorescence spectral line

    E-Print Network [OSTI]

    E. S. Andrianov; N. M. Chtchelkatchev; A. A. Pukhov

    2015-04-27T23:59:59.000Z

    We consider metamolecule consisting of bosonic mode correlated with the two-level system: it can be, for example, plasmonic mode interacting with the quantum dot or molecule in the resonator. We focus on the parameter range where all the correlations are strong and of the same order: interaction between bosonic mode correlated with the two-level system, external coherent drive and dissipation. Quantum Monte-Carlo simulations show a fluorescence of this system at dissipation larger than the driving amplitude and strong (by the order of magnitude) narrowing of its spectral line. This effect may be related to kind of a quantum stochastic resonance. We show that the fluorescence corresponds to finite domain over the coherent drive with sharp lower threshold and there is splitting of the Wigner function.

  9. DNA fragment sizing and sorting by laser-induced fluorescence

    DOE Patents [OSTI]

    Hammond, Mark L. (Angier, NC); Jett, James H. (Los Alamos, NM); Keller, Richard A. (Los Alamos, NM); Marrone, Babetta L. (Los Alamos, NM); Martin, John C. (Los Alamos, NM)

    1996-01-01T23:59:59.000Z

    A method is provided for sizing DNA fragments using high speed detection systems, such as flow cytometry to determine unique characteristics of DNA pieces from a sample. In one characterization the DNA piece is fragmented at preselected sites to produce a plurality of DNA fragments. The DNA piece or the resulting DNA fragments are treated with a dye effective to stain stoichiometrically the DNA piece or the DNA fragments. The fluorescence from the dye in the stained fragments is then examined to generate an output functionally related to the number of nucleotides in each one of the DNA fragments. In one embodiment, the intensity of the fluorescence emissions from each fragment is linearly related to the fragment length. The distribution of DNA fragment sizes forms a characterization of the DNA piece for use in forensic and research applications.

  10. Rotational order–disorder structure of fluorescent protein FP480

    SciTech Connect (OSTI)

    Pletnev, Sergei, E-mail: svp@ncifcrf.gov [SAIC-Frederick Inc., Basic Research Program, Argonne National Laboratory, 9700 South Cass Avenue, Argonne, IL 60439 (United States); Morozova, Kateryna S.; Verkhusha, Vladislav V. [Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461 (United States); Dauter, Zbigniew, E-mail: svp@ncifcrf.gov [Synchrotron Radiation Research Section, MCL, National Cancer Institute, Argonne National Laboratory, 9700 South Cass Avenue, Argonne, IL 60439 (United States); SAIC-Frederick Inc., Basic Research Program, Argonne National Laboratory, 9700 South Cass Avenue, Argonne, IL 60439 (United States)

    2009-09-01T23:59:59.000Z

    An analysis of the rotational order–disorder structure of fluorescent protein FP480 is presented. In the last decade, advances in instrumentation and software development have made crystallography a powerful tool in structural biology. Using this method, structural information can now be acquired from pathological crystals that would have been abandoned in earlier times. In this paper, the order–disorder (OD) structure of fluorescent protein FP480 is discussed. The structure is composed of tetramers with 222 symmetry incorporated into the lattice in two different ways, namely rotated 90° with respect to each other around the crystal c axis, with tetramer axes coincident with crystallographic twofold axes. The random distribution of alternatively oriented tetramers in the crystal creates a rotational OD structure with statistically averaged I422 symmetry, although the presence of very weak and diffuse additional reflections suggests that the randomness is only approximate.

  11. COMBINED FLUORESCENT AND GOLD PROBES FOR MICROSCOPIC AND MORPHOLOGICAL INVESTIGATIONS.

    SciTech Connect (OSTI)

    POWELL,R.D.HAINFELD,J.F.

    2002-04-17T23:59:59.000Z

    Nanogold{reg_sign}, a gold cluster with a core of gold atoms 1.4 nm in diameter, has proven to be a superior probe label for electron microscopy (EM), giving both higher labeling density and improved access to previously hindered or restricted antigens. It may be visualized by autometallography (AMG) for use in light microscopy (LM): silver-and gold-amplified Nanogold detection has proven to be one of the most sensitive methods available for the detection of low copy number targets such as viral DNA in cells and tissue specimens. AMG enhancement has also made Nanogold an effective detection label in blots and gels. The following protocols will be described: Labeling of nuclear components in cells. Protocol for in situ hybridization and detection with fluorescein-Nanogold--or Cy3{trademark}-Nanogold-labeled streptavidin. Nanogold is an inert molecule, and generally does not interact with biological molecules unless a specific chemical reactivity is introduced into the molecule. Conjugates are prepared using site-specific chemical conjugation through reactive chemical functionalities introduced during Nanogold preparation, which allows the gold label to be attached to a specific site on the conjugate biomolecule. For example, a maleimido-Nanogold derivative, which is specific for thiol binding, is frequently attached to the hinge region of an antibody at a unique thiol site generated by selective reduction of a hinge disulfide. This site is remote from the antigen combining region, and the Nanogold, therefore, does not compromise target binding. Nanogold may also be prepared with specific reactivity towards amines or other unique chemical groups. This mode of attachment enables the preparation of probes labeled with both Nanogold and fluorescent labels. Different chemical reactivities are used to attach the Nanogold and the fluorescent groups to different sites in the conjugate biomolecule, as shown in Figure 7.1. In this manner, the two labels are spaced sufficiently far apart that fluorescent resonance energy transfer does not quench the fluorescent signal, and the probes may be used to label specimens for fluorescent and EM observation in a single staining procedure. This reduces the complexity of the staining procedure, allowing less specimen perturbation, and also enables a higher degree of correlation between the fluorescence and EM localization of the target, thus increasing the usefulness of the complementary data sets. Since gold and fluorescent-labeled probes are often used at different concentrations under different conditions, optimum procedures for the use of fluorescent and gold probes may entail some degree of compromise between the most appropriate conditions for the two types of probes. However, the chemical stability of the Nanogold label means that it is generally stable to a wide range of use conditions, and the following protocols have been found to be effective for labeling specimens with combined fluorescein and Nanogold-labeled antibody Fab' probes and with combined Cy3 and Nanogold-labeled streptavidin.

  12. In-situ stoichiometry determination using x-ray fluorescence generated by reflection-high-energy-electron-diffraction

    SciTech Connect (OSTI)

    Keenan, Cameron; Chandril, Sandeep; Lederman, David [Department of Physics and Multifunctional Materials Laboratory, West Virginia University, Morgantown, West Virginia 26506 (United States); Myers, T. H. [Department of Physics and Multifunctional Materials Laboratory, West Virginia University, Morgantown, West Virginia 26506 (United States); Materials Science, Engineering, and Commercialization Program, Texas State University-San Marcos, San Marcos, Texas 78666 (United States)

    2011-06-01T23:59:59.000Z

    A major challenge in the stoichiometric growth of complex oxide compounds is the control of the relative compositions of the constituent materials. A potential avenue for compositional analysis during growth is the use of x-ray fluorescence generated during reflection high energy electron diffraction measurements. Using this technique, relative compositions of Y and Mn in molecular beam epitaxy grown YMnO{sub 3} samples were studied. Comparing the results with Rutherford back scattering spectroscopy suggests that the technique has the potential for real-time analysis of elemental fluxes and stoichiometry control during sample growth.

  13. Development of a fluorescent antibody test for equine infectious anemia

    E-Print Network [OSTI]

    Lester, Thomas Lee

    1972-01-01T23:59:59.000Z

    . Moore The development of a direct fluorescent antibody technique for a reliable laboratory and diagnostic test for equine infectious anemia (EIA) was described. A specific antiserum was produced by inoculation of goats with EIA virus derived from... the Texas and Illinois strains of EIA virus in cell culture and in known infected horses and against the Wyoming strain of EIA virus in inoculated horses. Its specificity was further substantiated by testing it against heterologous viruses including...

  14. Contraband Detection with Nuclear Resonance Fluorescence: Feasibility and Impact

    SciTech Connect (OSTI)

    Pruet, J; Lange, D

    2007-01-03T23:59:59.000Z

    In this report they show that cargo interrogation systems developed to thwart trafficking of illicit nuclear materials could also be powerful tools in the larger fight against contraband smuggling. In particular, in addition to detecting special nuclear materials, cargo scanning systems that exploit nuclear resonance fluorescence to detect specific isotopes can be used to help find: chemical weapons; some drugs as well as some chemicals regulated under the controlled substances act; precious metals; materials regulated under export control laws; and commonly trafficked fluorocarbons.

  15. Dynamic fluorescence imaging with molecular agents for cancer detection

    E-Print Network [OSTI]

    Kwon, Sun Kuk

    2009-05-15T23:59:59.000Z

    emission computed tomography SUV standard uptake values SLN sentinel lymph node TK tyrosine kinase TBR target to background ratio Symbols ? pharmacokinetic parameter ? pharmacokinetic parameter )(tC BOUND concentration of dye... bound to integrin receptor either in the vascular or extravascular compartments )(tC LAREXTRAVASCU concentration of dye in extravascular space )(tC VASCULAR concentration of dye in vascular space 0 I background fluorescence intensity c k...

  16. Nuclear Resonance Fluorescence and Isotopic Mapping of Containers

    SciTech Connect (OSTI)

    Johnson, M S; McNabb, D P

    2008-08-08T23:59:59.000Z

    National security programs have expressed interest in developing systems to isotopically map shipping containers, fuel assemblies, and waste barrels for various materials including special nuclear material (SNM). Current radiographic systems offer little more than an ambiguous density silhouette of a container's contents. In this paper we will present a system being developed at LLNL to isotopically map containers using the nuclear resonance fluorescence (NRF) method. Recent experimental measurements on NRF strengths in SNM are discussed.

  17. Comparing and Contrasting Health and

    E-Print Network [OSTI]

    Entekhabi, Dara

    / private partnership Institutional Change In Health: · The Affordable Care Act (ACA) · The recognitionComparing and Contrasting Health and Transportation as Complex Sociotechnical Systems System: Comparing and Contrasting Health and Transportation as Complex Sociotechnical Systems Ideas about

  18. Enhanced detection of fluorescence quenching in labeled cells

    DOE Patents [OSTI]

    Crissman, H.A.; Steinkamp, J.A.

    1987-11-30T23:59:59.000Z

    A method is provided for quantifying BrdU labeled DNA in cells. The BrdU is substituted onto the DNA and the DNA is stained with a first fluorochrome having a fluorescence which is quenchable by BrdU. The first fluorochrome is preferably a thymidine base halogen analogue, such as a Hoechst fluorochrome. The DNA is then stained with a second fluorochrome having a fluorescence which is substantially uneffected by BrdU. The second fluorochrome may be selected from the group consisting of mithramycin, chromomycin A3, olivomycin, propidium iodide and ethidium bromine. The fluorescence from the first and second fluorochromes is then measured to obtain first and second output signals, respectively. The first output signal is subtracted from the second output signal to obtain a difference signal which is functionally related to the quantity of BrdU incorporated into DNA. The technique is particularly useful for quantifying the synthesis of DNA during the S-phase of the cell cycle. 2 figs.

  19. ISSUANCE 2014-12-29: Energy Conservation Program: Clarification for Energy Conservation Standards and Test Procedures for Fluorescent Lamp Ballasts

    Broader source: Energy.gov [DOE]

    Energy Conservation Program: Clarification for Energy Conservation Standards and Test Procedures for Fluorescent Lamp Ballasts

  20. Characterization of dissolved organic matter fluorescence in the South Atlantic Bight with use of PARAFAC model: Relationships between fluorescence and its

    E-Print Network [OSTI]

    Durako, Michael J.

    [aCDOM(350)] and CDOM excitation emission matrix (EEM) fluorescence were used to estimate annual (PARAFAC) of CDOM EEM spectra identified six components: three terrestrial humic-like, one marine humic concentration and aCDOM(350), total fluorescence intensity and the intensities of respective EEM components. DOC

  1. Helical tomotherapy with dynamic running-start-stop delivery compared to conventional tomotherapy delivery

    SciTech Connect (OSTI)

    Rong, Yi, E-mail: yi.rong@osumc.edu [Department of Radiation Oncology, The Ohio State University Wexner Medical Center, Columbus, Ohio 43210 (United States)] [Department of Radiation Oncology, The Ohio State University Wexner Medical Center, Columbus, Ohio 43210 (United States); Chen, Yu; Lu, Weiguo [21st Century Oncology, Madison, Wisconsin 53719 (United States)] [21st Century Oncology, Madison, Wisconsin 53719 (United States); Shang, Lu [Guangxi Polytechnic of Construction and Technology, Nanning (China)] [Guangxi Polytechnic of Construction and Technology, Nanning (China); Zuo, Li [Radiologic Sciences and Respiratory Therapy Division, School of Health and Rehabilitation Sciences, The Ohio State University College of Medicine, Columbus, Ohio 43210 (United States)] [Radiologic Sciences and Respiratory Therapy Division, School of Health and Rehabilitation Sciences, The Ohio State University College of Medicine, Columbus, Ohio 43210 (United States); Chen, Quan [Department of Radiation Oncology, University of Virginia, Charlottesville, Virginia 22904 (United States)] [Department of Radiation Oncology, University of Virginia, Charlottesville, Virginia 22904 (United States)

    2014-05-15T23:59:59.000Z

    Purpose: Despite superior target dose uniformity, helical tomotherapy{sup ®} (HT) may involve a trade-off between longitudinal dose conformity and beam-on time (BOT), due to the limitation of only three available jaw sizes with the conventional HT (1.0, 2.5, and 5.0 cm). The recently introduced dynamic running-start-stop (RSS) delivery allows smaller jaw opening at the superior and inferior ends of the target when a sharp penumbra is needed. This study compared the dosimetric performance of RSS delivery with the fixed jaw HT delivery. Methods: Twenty patient cases were selected and deidentified prior to treatment planning, including 16 common clinical cases (brain, head and neck (HN), lung, and prostate) and four special cases of whole brain with hippocampus avoidance (WBHA) that require a high degree of dose modulation. HT plans were generated for common clinical cases using the fixed 2.5 cm jaw width (HT2.5) and WBHA cases using 1.0 cm (HT1.0). The jaw widths for RSS were preset with a larger size (RSS5.0 vs HT2.5 and RSS2.5 vs HT1.0). Both delivery techniques were planned based on identical contours, prescriptions, and planning objectives. Dose indices for targets and critical organs were compared using dose-volume histograms, BOT, and monitor units. Results: The average BOT was reduced from 4.8 min with HT2.5 to 2.5 min with RSS5.0. Target dose homogeneity with RSS5.0 was shown comparable to HT2.5 for common clinical sites. Superior normal tissue sparing was observed in RSS5.0 for optic nerves and optic chiasm in brain and HN cases. RSS5.0 demonstrated improved dose sparing for cord and esophagus in lung cases, as well as penile bulb in prostate cases. The mean body dose was comparable for both techniques. For the WBHA cases, the target homogeneity was significantly degraded in RSS2.5 without distinct dose sparing for hippocampus, compared to HT1.0. Conclusions: Compared to the fixed jaw HT delivery, RSS combined with a larger jaw width provides faster treatment delivery and improved cranial-caudal target dose conformity. The target coverage achieved by RSS with a large jaw width is comparable to the fixed jaw HT delivery for common cancer sites, but may deteriorate for cases where complex geometry is present in the middle part of the target.

  2. A light diet for a giant appetite: An assessment of China's proposed fluorescent lamp standard

    SciTech Connect (OSTI)

    Lin, Jiang

    2002-04-11T23:59:59.000Z

    Lighting has been one of the fastest growing electric end-uses in China over the last twenty years, with an average annual growth rate of 14%. Fluorescent lighting provides a significant portion of China's lighting need. In 1998, China produced 680 million fluorescent lamps, of which 420 million were linear fluorescent lamps of various diameters (T8 to T12). There are substantial variations both in energy efficiency and lighting performance among locally produced fluorescent lamps. Such variations present a perfect opportunity for policy intervention through efficiency standards to promote the adoption of more efficient fluorescent lamps in China. This paper analyzes China's proposed minimum efficiency standard for fluorescent lamps and presents an assessment of its likely impacts on China's lighting energy consumption and GHG emissions.

  3. Air Fluorescence Relevant for Cosmic-Ray Detection - Review of Pioneering Measurements

    E-Print Network [OSTI]

    Fernando Arqueros; Joerg R. Hoerandel; Bianca Keilhauer

    2008-07-24T23:59:59.000Z

    Cosmic rays with energies exceeding $10^{17}$ eV are frequently registered by measurements of the fluorescence light emitted by extensive air showers. The main uncertainty for the absolute energy scale of the measured air showers is coming from the fluorescence light yield of electrons in air. The fluorescence light yield has been studied in laboratory experiments. Pioneering measurements between 1954 and 2000 are reviewed.

  4. Microcavity effects on the generation,fluorescence, and diffusion of excitons in organic solar cells

    E-Print Network [OSTI]

    Kozyreff, G; Vuong, L T; Silleras, O Nieto; Martorell, J

    2013-01-01T23:59:59.000Z

    We compute the short-circuit diffusion current of excitons in an organic solar cell, with special emphasis on fluorescence losses. The exciton diffusion length is not uniform but varies with its position within the device, even with moderate fluorescence quantum efficiency. With large quantum efficiencies, the rate of fluorescence can be strongly reduced with proper choices of the geometrical and dielectric parameters. In this way, the diffusion length can be increased and the device performance significantly improved.

  5. COMPARATIVE MEDICINE LABORATORY ANIMAL FACILITIES

    E-Print Network [OSTI]

    Krovi, Venkat

    5.A.6 COMPARATIVE MEDICINE LABORATORY ANIMAL FACILITIES STANDARD OPERATING PROCEDURE for REPORTING PHYSICAL PLANT AND ENVIRONMENTAL CONDITIONS ABNORMALITIES AT THE COMPARATIVE MEDICINE LABORATORY ANIMAL investigator to keep her/him informed of the progress or resolution of the problem. #12;

  6. ALGORITHMS FOR CONSTRUCTING COMPARATIVE MAPS

    E-Print Network [OSTI]

    Kleinberg, Jon

    (i.e. that the segments are homeologous). The construction of comparative maps between the genomes and phylogenetic relationships among species as well as fundamental principles of genome evolution. Comparative maps allow us to use structural and functional information about one genome to make predictions about

  7. Feedback control of the squeezing of the fluorescence light

    E-Print Network [OSTI]

    Alberto Barchielli; Matteo Gregoratti

    2009-10-09T23:59:59.000Z

    Among the formulations of the theory of quantum measurements in continuous time, quantum trajectory theory is very suitable for the introduction of measurement based feedback and closed loop control of quantum systems. In this paper we present such a construction in the concrete case of a two-level atom stimulated by a coherent, monochromatic laser. In particular, we show how fast feedback \\`a la Wiseman and Milburn can be introduced in the formulation of the theory. Then, the spectrum of the free fluorescence light is studied and typical quantum phenomena, squeezing and sub-natural line-narrowing, are presented.

  8. Quantum Process Tomography by 2D Fluorescence Spectroscopy

    E-Print Network [OSTI]

    Leonardo A. Pachon; Andrew H. Marcus; Alan Aspuru-Guzik

    2015-02-09T23:59:59.000Z

    Reconstruction of the dynamics (quantum process tomography) of the single-exciton manifold in energy transfer systems is proposed here on the basis of two-dimensional fluorescence spectroscopy (2D-FS) with phase-modulation. The quantum-process-tomography protocol introduced here benefits from, e.g., the sensitivity enhancement and signal-to-noise ratio ascribed to 2D-FS. Although the isotropically averaged spectroscopic signals depend on the quantum yield parameter $\\Gamma$ of the doubly-excited-exciton manifold, it is shown that the reconstruction of the dynamics is insensitive to this parameter. Applications to foundational and applied problems, as well as further extensions, are discussed.

  9. Quantum Process Tomography by 2D Fluorescence Spectroscopy

    E-Print Network [OSTI]

    Pachon, Leonardo A; Aspuru-Guzik, Alan

    2015-01-01T23:59:59.000Z

    Reconstruction of the dynamics (quantum process tomography) of the single-exciton manifold in energy transfer systems is proposed here on the basis of two-dimensional fluorescence spectroscopy (2D-FS) with phase-modulation. The quantum-process-tomography protocol introduced here benefits from, e.g., the sensitivity enhancement and signal-to-noise ratio ascribed to 2D-FS. Although the isotropically averaged spectroscopic signals depend on the quantum yield parameter $\\Gamma$ of the doubly-excited-exciton manifold, it is shown that the reconstruction of the dynamics is insensitive to this parameter. Applications to foundational and applied problems, as well as further extensions, are discussed.

  10. Interaction of fluorescent probes with dodecylammonium propionate aggregates in cyclohexane

    E-Print Network [OSTI]

    Correll, Glenn David

    1975-01-01T23:59:59.000Z

    of Added . 01M NaOEL 44 48 51 54 25. Rate P1ot of Carbontetrachloride Quenching of PSA in DAP/Cyclohexane. 26. Rate Plots of KSr Quenching of PSA. in DAP/Cyclohexane 58 at Different Stoichiometric Concentrations of Hs0. . . . . 61 27. KBr... Quenching Rate vs. Aqueous Volume. . . . . . . . . . . . . 28. PSA Fluorescence Lifetimes vs. Aqueous Volume of Constant KBr Concentration (4. OM KBr/0. 01M NaOH). . . 29. Plot of' ?. /(I- ?. ) vs. [DAP] for PSA in Cyclohexane at Constant Water...

  11. Blue-green phosphor for fluorescent lighting applications

    DOE Patents [OSTI]

    Srivastava, Alok; Comanzo, Holly; Manivannan, Venkatesan; Setlur, Anant Achyut

    2005-03-15T23:59:59.000Z

    A fluorescent lamp including a phosphor layer including Sr.sub.4 Al.sub.14 O.sub.25 :Eu.sup.2+ (SAE) and at least one of each of a red, green and blue emitting phosphor. The phosphor layer can optionally include an additional, deep red phosphor and a yellow emitting phosphor. The resulting lamp will exhibit a white light having a color rendering index of 90 or higher with a correlated color temperature of from 2500 to 10000 Kelvin. The use of SAE in phosphor blends of lamps results in high CRI light sources with increased stability and acceptable lumen maintenance over, the course of the lamp life.

  12. Product Standards for Fluorescent Lighting (Japan) | Open Energy

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to: navigation,Pillar Group BV Jump to: navigation,PowerInformation Fluorescent Lighting

  13. EA-1881: Energy Conservation Program: Energy Conservation Standards for Fluorescent Lamp Ballasts

    Broader source: Energy.gov [DOE]

    This EA evaluates the environmental impacts of a proposal to amend energy conservation standards for various consumer products and certain commercial and industrial equipment, including fluorescent lamp ballasts.

  14. High power light emitting diode based setup for photobleaching fluorescent impurities

    E-Print Network [OSTI]

    Kaufman, Laura

    High power light emitting diode based setup for photobleaching fluorescent impurities Tobias K be photobleached before final sample preparation. The instrument consists of high power light emitting diodes

  15. Nuclear Resonance Fluorescence to Measure Plutonium Mass in Spent Nuclear Fuel

    E-Print Network [OSTI]

    Ludewigt, Bernhard A

    2011-01-01T23:59:59.000Z

    and Nuclear Recoil . . . . . . . . . . . . . . . . . . . . .2 Quantitative Measurements using NRF 2.1 Nuclear ResonanceFuture Work A Transmission Nuclear Resonance Fluorescence

  16. Volume 20. number 3 FEBS LETTERS February 1972 FLUORESCENCE AND DELAYED LIGHT EMISSION IN

    E-Print Network [OSTI]

    Govindjee

    : dichloroplenol indophenol; DLE: delayed light emission; NADP+: nicotinamide adenine dinucleotide phosphate; PDA (DLE) that of Jursinic and Govindjee [4]. For mea- surements of the recovery of fluorescence transients

  17. Graphene Signal Amplification for Sensitive and Real-Time Fluorescence Anisotropy Detection of Small Molecules

    E-Print Network [OSTI]

    Tan, Weihong

    Graphene Signal Amplification for Sensitive and Real-Time Fluorescence Anisotropy Detection graphene oxide (GO) as the signal amplifier. Because of the extraordinarily larger volume of GO

  18. Fluorescent properties of c-type cytochromes reveal their potential role as an extracytoplasmic electron

    E-Print Network [OSTI]

    Lovley, Derek

    via fluorescence microscopy, and the cyto- chromes in a G. sulfurreducens biofilm, remotely excited communities have indi- cated that Geobacter species are the predominant metal-reducing microorganisms

  19. Excitation-emission spectra and fluorescence quantum yields for fresh and aged biogenic secondary organic aerosols

    SciTech Connect (OSTI)

    Lee, Hyun Ji; Laskin, Alexander; Laskin, Julia; Nizkorodov, Sergey A.

    2013-05-10T23:59:59.000Z

    Certain biogenic secondary organic aerosols (SOA) become absorbent and fluorescent when exposed to reduced nitrogen compounds such as ammonia, amines and their salts. Fluorescent SOA may potentially be mistaken for biological particles by detection methods relying on fluorescence. This work quantifies the spectral distribution and effective quantum yields of fluorescence of SOA generated from two monoterpenes, limonene and a-pinene, and two different oxidants, ozone (O3) and hydroxyl radical (OH). The SOA was generated in a smog chamber, collected on substrates, and aged by exposure to ~100 ppb ammonia vapor in air saturated with water vapor. Absorption and excitation-emission matrix (EEM) spectra of aqueous extracts of aged and control SOA samples were measured, and the effective absorption coefficients and fluorescence quantum yields (~0.005 for 349 nm excitation) were determined from the data. The strongest fluorescence for the limonene-derived SOA was observed for excitation = 420+- 50 nm and emission = 475 +- 38 nm. The window of the strongest fluorescence shifted to excitation = 320 +- 25 nm and emission = 425 +- 38 nm for the a-pinene-derived SOA. Both regions overlap with the excitation-emission matrix (EEM) spectra of some of the fluorophores found in primary biological aerosols. Our study suggests that, despite the low quantum yield, the aged SOA particles should have sufficient fluorescence intensities to interfere with the fluorescence detection of common bioaerosols.

  20. Capillary electrophoresis-fluorescence line narrowing system (CE-FLNS) for on-line structural characterization

    DOE Patents [OSTI]

    Jankowiak, R.J.; Small, G.J.; Shields, P.A.

    1999-04-27T23:59:59.000Z

    Capillary electrophoresis (CE) is interfaced with low temperature fluorescence line-narrowing (FLN) spectroscopy for on-line structural characterization of separated molecular analytes. 21 figs.

  1. Capillary electrophoresis-fluorescence line narrowing system (CE-FLNS) for on-line structural characterization

    DOE Patents [OSTI]

    Jankowiak, Ryszard J. (Ames, IA); Small, Gerald J. (Ames, IA); Shields, Peter A. (Reading, MA)

    1999-04-27T23:59:59.000Z

    Capillary electrophoresis (CE) is interfaced with low temperature fluorescence line-narrowing (FLN) spectroscopy for on-line structural characterization of separated molecular analytes.

  2. AUTOMATED ELEMENTAL ANALYSIS USING ENERGY DISPERSIVE X-RAY FLUORESCENCE ANALYSIS

    E-Print Network [OSTI]

    Jaklevic, J.M.

    2011-01-01T23:59:59.000Z

    upon the x-ray fluorescence (XRF) method for the elementalnon-destructive nature of XRF is especially important whereconcentrations are measured in the XRF spectrometer and the

  3. Coral fluorescence and symbiosis : photoacclimation, thermal shock, life history changes, and implications for reef monitoring

    E-Print Network [OSTI]

    Roth, Melissa Susan

    2010-01-01T23:59:59.000Z

    Diversity and evolution of coral fluorescent proteins. PLoSB. (2008). Climate change and coral reef bleaching: Anpigment in reef-building corals. Mar. Ecol. Prog. Ser. 364,

  4. Fluorescence-based detection methodologies for nitric oxide using transition metal scaffolds

    E-Print Network [OSTI]

    Hilderbrand, Scott A. (Scott Alan), 1976-

    2004-01-01T23:59:59.000Z

    Chapter 1. Fluorescence-Based Detection Methodologies for Nitric Oxide: A Review. Chapter 2. Cobalt Chemistry with Mixed Aminotroponimine Salicylaldimine Ligands: Synthesis, Characterization, and Nitric Oxide Reactivity. ...

  5. Lung Cancer (2005) 47, 41--47 Improving the specificity of fluorescence

    E-Print Network [OSTI]

    Duin, Robert P.W.

    2005-01-01T23:59:59.000Z

    Lung Cancer (2005) 47, 41--47 Improving the specificity of fluorescence bronchoscopy spectroscopy; Lung cancer; Bronchoscopy Summary Detection of malignancies of the bronchial tree in an early

  6. Ceramic Mugs & Dishes Incandescent Light Bulbs

    E-Print Network [OSTI]

    Yamamoto, Keith

    , MU East Rock Hall/19-B CELL PHONES - EYEGLASSES 654 Minnesota Street Room 208, copy room CVRI Helen. Zion Cancer Research Building N423 Parnassus Campus: eyeglasses "I" level, Optometry Store, MU West

  7. Intensity-modulated radiotherapy improves lymph node coverage and dose to critical structures compared with three-dimensional conformal radiation therapy in clinically localized prostate cancer

    SciTech Connect (OSTI)

    Wang-Chesebro, Alice [Radiation Oncology, University of California-San Francisco, San Francisco, CA (United States)]. E-mail: awang@radonc17.ucsf.edu; Xia Ping [Radiation Oncology, University of California-San Francisco, San Francisco, CA (United States); Coleman, Joy [Radiation Oncology, University of California-San Francisco, San Francisco, CA (United States); Akazawa, Clayton C. [Radiation Oncology, University of California-San Francisco, San Francisco, CA (United States); Roach, Mack [Radiation Oncology, University of California-San Francisco, San Francisco, CA (United States)

    2006-11-01T23:59:59.000Z

    Purpose: The aim of this study was to quantify gains in lymph node coverage and critical structure dose reduction for whole-pelvis (WP) and extended-field (EF) radiotherapy in prostate cancer using intensity-modulated radiotherapy (IMRT) compared with three-dimensional conformal radiotherapy (3DCRT) for the first treatment phase of 45 Gy in the concurrent treatment of lymph nodes and prostate. Methods and Materials: From January to August 2005, 35 patients with localized prostate cancer were treated with pelvic IMRT; 7 had nodes defined up to L5-S1 (Group 1), and 28 had nodes defined above L5-S1 (Group 2). Each patient had 2 plans retrospectively generated: 1 WP 3DCRT plan using bony landmarks, and 1 EF 3DCRT plan to cover the vascular defined volumes. Dose-volume histograms for the lymph nodes, rectum, bladder, small bowel, and penile bulb were compared by group. Results: For Group 1, WP 3DCRT missed 25% of pelvic nodes with the prescribed dose 45 Gy and missed 18% with the 95% prescribed dose 42.75 Gy, whereas WP IMRT achieved V{sub 45Gy} = 98% and V{sub 42.75Gy} = 100%. Compared with WP 3DCRT, IMRT reduced bladder V{sub 45Gy} by 78%, rectum V{sub 45Gy} by 48%, and small bowel V{sub 45Gy} by 232 cm{sup 3}. EF 3DCRT achieved 95% coverage of nodes for all patients at high cost to critical structures. For Group 2, IMRT decreased bladder V{sub 45Gy} by 90%, rectum V{sub 45Gy} by 54% and small bowel V{sub 45Gy} by 455 cm{sup 3} compared with EF 3DCRT. Conclusion: In this study WP 3DCRT missed a significant percentage of pelvic nodes. Although EF 3DCRT achieved 95% pelvic nodal coverage, it increased critical structure doses. IMRT improved pelvic nodal coverage while decreasing dose to bladder, rectum, small bowel, and penile bulb. For patients with extended node involvement, IMRT especially decreases small bowel dose.

  8. Protein- protein interaction detection system using fluorescent protein microdomains

    DOE Patents [OSTI]

    Waldo, Geoffrey S. (Santa Fe, NM); Cabantous, Stephanie (Los Alamos, NM)

    2010-02-23T23:59:59.000Z

    The invention provides a protein labeling and interaction detection system based on engineered fragments of fluorescent and chromophoric proteins that require fused interacting polypeptides to drive the association of the fragments, and further are soluble and stable, and do not change the solubility of polypeptides to which they are fused. In one embodiment, a test protein X is fused to a sixteen amino acid fragment of GFP (.beta.-strand 10, amino acids 198-214), engineered to not perturb fusion protein solubility. A second test protein Y is fused to a sixteen amino acid fragment of GFP (.beta.-strand 11, amino acids 215-230), engineered to not perturb fusion protein solubility. When X and Y interact, they bring the GFP strands into proximity, and are detected by complementation with a third GFP fragment consisting of GFP amino acids 1-198 (strands 1-9). When GFP strands 10 and 11 are held together by interaction of protein X and Y, they spontaneous association with GFP strands 1-9, resulting in structural complementation, folding, and concomitant GFP fluorescence.

  9. A QUANTUM BAND MODEL OF THE {nu}{sub 3} FUNDAMENTAL OF METHANOL (CH{sub 3}OH) AND ITS APPLICATION TO FLUORESCENCE SPECTRA OF COMETS

    SciTech Connect (OSTI)

    Villanueva, G. L.; DiSanti, M. A.; Mumma, M. J. [Solar System Exploration Division, Mailstop 690.3, NASA Goddard Space Flight Center, Greenbelt, MD 20771 (United States); Xu, L.-H., E-mail: Geronimo.Villanueva@nasa.gov [Department of Physics and Centre for Laser, Atomic, and Molecular Sciences, University of New Brunswick, Saint John, New Brunswick E2L 4L5 (Canada)

    2012-03-01T23:59:59.000Z

    Methanol (CH{sub 3}OH) radiates efficiently at infrared wavelengths, dominating the C-H stretching region in comets, yet inadequate quantum-mechanical models have imposed limits on the practical use of its emission spectra. Accordingly, we constructed a new line-by-line model for the {nu}{sub 3} fundamental band of methanol at 2844 cm{sup -1} (3.52 {mu}m) and applied it to interpret cometary fluorescence spectra. The new model permits accurate synthesis of line-by-line spectra for a wide range of rotational temperatures, ranging from 10 K to more than 400 K. We validated the model by comparing simulations of CH{sub 3}OH fluorescent emission with measured spectra of three comets (C/2001 A2 LINEAR, C/2004 Q2 Machholz and 8P/Tuttle) acquired with high-resolution infrared spectrometers at high-altitude sites. The new model accurately describes the complex emission spectrum of the {nu}{sub 3} band, providing distinct rotational temperatures and production rates at greatly improved confidence levels compared with results derived from earlier fluorescence models. The new model reconciles production rates measured at infrared and radio wavelengths in C/2001 A2 (LINEAR). Methanol can now be quantified with unprecedented precision and accuracy in astrophysical sources through high-dispersion spectroscopy at infrared wavelengths.

  10. Enhancer Identification through Comparative Genomics

    E-Print Network [OSTI]

    Visel, Axel; Bristow, James; Pennacchio, Len A.

    2006-01-01T23:59:59.000Z

    through Comparative Genomics Axel Visel, James Bristow andWalnut Creek, CA 94598 USA. Genomics Division, MS 84-171,Len A. Pennacchio, Genomics Division, One Cyclotron Road, MS

  11. 4, 43254360, 2007 Comparing model

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    HESSD 4, 4325­4360, 2007 Comparing model performance in the Rhine basin A. H. te Linde et al. Title different atmospheric forcing data sets A. H. te Linde 1 , J. C. J. H. Aerts 1 , R. T. W. L. Hurkmans 2 Correspondence to: A. H. te Linde (aline.te.linde@ivm.vu.nl) 4325 #12;HESSD 4, 4325­4360, 2007 Comparing model

  12. Synthesis and Characterization of Novel Fluorescent Injectable Micro-Carriers for Tissue Regeneration 

    E-Print Network [OSTI]

    Arora, Akshi

    2014-04-28T23:59:59.000Z

    of sacrificing the animals or undertaking elaborate histological procedures. To date, there is no report on the synthesis of fluorescent PLLA. In this research, we aim to develop an injectable fluorescent PLLA scaffold for tissue regeneration by using Eosin Y (EY...

  13. FISH and Chips: Automation of Fluorescent Dot Counting in Interphase Cell Nuclei

    E-Print Network [OSTI]

    van Vliet, Lucas J.

    ) in each cell nucleus. This system works with two fluorescent dyes, one for the DNA hybridization dotsFISH and Chips: Automation of Fluorescent Dot Counting in Interphase Cell Nuclei Hans Netten,1 Ian abnormalities in inter- phase cell nuclei. This process is called dot counting. To estimate the distribution

  14. Demonstration of Fluorescent RGB Electrowetting Devices for Light Wave Coupling Displays

    E-Print Network [OSTI]

    Steckl, Andrew J.

    and wasted, instead of recycling the light for use at pixels in the ON state. We present the demonstration-degrade the organic fluorescent oils. Violet light sources include custom developed cold-cathode- fluorescent lamps of a traditional diffuse backlight because (as will be discussed later) it allows for the advantage of recycling

  15. A Behavioral SPICE Compatible Model of an Electrodeless Fluorescent Lamp Sam Ben-Yaakov*1

    E-Print Network [OSTI]

    to the plasma. In the case of the lamp with electrodes, coupling is via wires. In the case of the electrodelessA Behavioral SPICE Compatible Model of an Electrodeless Fluorescent Lamp Sam Ben-Yaakov*1 , Moshe, SPICE compatible, model was developed for an electrodeless fluorescent lamp (OSRAM SYLVANIA ICETRON

  16. Nucleic acid encoding a self-assembling split-fluorescent protein system

    DOE Patents [OSTI]

    Waldo, Geoffrey S; Cabantous, Stephanie

    2014-04-01T23:59:59.000Z

    The invention provides a protein labeling and detection system based on self-complementing fragments of fluorescent and chromophoric proteins. The system of the invention is exemplified with various combinations of self-complementing fragments derived from Aequorea victoria Green Fluorescent Protein (GFP), which are used to detect and quantify protein solubility in multiple assay formats, both in vitro and in vivo.

  17. Directed evolution methods for improving polypeptide folding and solubility and superfolder fluorescent proteins generated thereby

    DOE Patents [OSTI]

    Waldo, Geoffrey S. (Santa Fe, NM)

    2007-09-18T23:59:59.000Z

    The current invention provides methods of improving folding of polypeptides using a poorly folding domain as a component of a fusion protein comprising the poorly folding domain and a polypeptide of interest to be improved. The invention also provides novel green fluorescent proteins (GFPs) and red fluorescent proteins that have enhanced folding properties.

  18. Nucleic acid encoding a self-assembling split-fluorescent protein system

    DOE Patents [OSTI]

    Waldo, Geoffrey S. (Santa Fe, NM); Cabantous, Stephanie (Los Alamos, NM)

    2011-06-07T23:59:59.000Z

    The invention provides a protein labeling and detection system based on self-complementing fragments of fluorescent and chromophoric proteins. The system of the invention is exemplified with various combinations of self-complementing fragments derived from Aequorea victoria Green Fluorescent Protein (GFP), which are used to detect and quantify protein solubility in multiple assay formats, both in vitro and in vivo.

  19. [16) Green Fluorescent Protein Chimeras to Probe Protein-Protein Interactions

    E-Print Network [OSTI]

    Raines, Ronald T.

    [16) Green Fluorescent Protein Chimeras to Probe Protein-Protein Interactions By SANG-HYUN PARK of reproduction in any form reserved. 0076-6879/00 $30.00 #12;252 A B OTHER APPROACHES USING CHIMERAS c ~S65T GFPHDII M 66 .......... 4S,.!;. 31 ui' 14 ..... [lB) FIG. 1. Green fluorescent protein chimera. (A

  20. Fluorescence Assay 2. http://www.tgrbio.com/cancer-cell-lines-primary-cell-

    E-Print Network [OSTI]

    Collins, Gary S.

    hazardous waste. Fluorescence microscopy is a powerful tool with a broad scope of applications easily fluorescence microscopy. In creating an innovative protocol for the 5-HT3a receptor, it is necessary-HT3a Expression Purification/Characterization While creating a protocol for protein expression

  1. Ultrafast Proton Shuttling in Psammocora Cyan Fluorescent Protein John T. M. Kennis,*,

    E-Print Network [OSTI]

    van Stokkum, Ivo

    Ultrafast Proton Shuttling in Psammocora Cyan Fluorescent Protein John T. M. Kennis,*, Ivo H. M-state proton transfer (ESPT) reactions. Recently, a novel cyan fluorescent protein (CFP) termed psamFP488 vicinity to the chromophore to act as a proton acceptor. Our findings support a model where unusually fast

  2. Transition in the Temperature-Dependence of GFP Fluorescence: From Proton Wires to Proton Exit

    E-Print Network [OSTI]

    Agmon, Noam

    Transition in the Temperature-Dependence of GFP Fluorescence: From Proton Wires to Proton Exit protein, photo-excitation leads to excited-state proton transfer from its chromophore, leaving behind a strongly fluorescing anion, while the proton is commonly thought to migrate internally to Glu-222. X

  3. Fluorescence diagnostics of oil pollution in coastal marine waters by use of artificial neural networks

    E-Print Network [OSTI]

    Oldenburg, Carl von Ossietzky Universität

    marine waters with fluorescence spectroscopy and of using artificial neural networks for data interpre with an artificial neural network. The results demonstrate the possibility of estimating oil concentrationsFluorescence diagnostics of oil pollution in coastal marine waters by use of artificial neural

  4. Optical fiber-based fluorescent viscosity sensor Mark A. Haidekker and Walter J. Akers

    E-Print Network [OSTI]

    Theodorakis, Emmanuel

    Optical fiber-based fluorescent viscosity sensor Mark A. Haidekker and Walter J. Akers Department to molecular rotors in solution. An optical fiber-based fluorescent vis- cosity sensor may be used in real, we sought to develop an optical fiber-based sensor that could re- port changes in fluid viscosity

  5. Improving the photostability of bright monomeric orange and red fluorescent proteins

    E-Print Network [OSTI]

    Tsien, Roger Y.

    Improving the photostability of bright monomeric orange and red fluorescent proteins Nathan C illumination. Although fluorescent proteins typically bleach at a substantially slower rate than many small-molecule dyes, in many cases the lack of sufficient photostability remains an important limiting factor

  6. Micro-Raman Spectroscopy of Algae: Composition Analysis and Fluorescence Background Behavior

    E-Print Network [OSTI]

    ARTICLE Micro-Raman Spectroscopy of Algae: Composition Analysis and Fluorescence Background performed using Stokes Raman scattering for compositional analysis of algae. Two algal species, Chlorella while acquiring Raman signals from the algae. The time dependence of fluorescence background is char

  7. Volume 57, number 3 FEBS LETTERS October 1975 INTERACTIONS OF FLUORESCENT ANALOGS OF ADENINE NUCLEOTIDES

    E-Print Network [OSTI]

    Govindjee

    NUCLEOTIDES WITH COUPLING FACTOR PROTEIN ISOLATED FROM SPINACH CHLOROPLASTS David L. VANDERMEULEN by Farron [ 151, was used in determining its molarity. The fluorescent nucleotides 1,N6ethenoadenosine mono fluorescent nucleotides (mol. wt. G600) bind to the relatively large (mol. wt. = 325 000), slowly rotating CFI

  8. Photocleavable fluorescent nucleotides for DNA sequencing on a chip constructed by site-specific

    E-Print Network [OSTI]

    Church, George M.

    Photocleavable fluorescent nucleotides for DNA sequencing on a chip constructed by site sequencing by synthesis using photocleav- able (PC) fluorescent nucleotides [dUTP-PC-4,4-difluoro-4-bora-3 ,4 nucleotides to identify 7 bases in the DNA template. These results demonstrate that the PC nucleotide

  9. Spectroscopic Properties of a Self-Assembled Zinc Porphyrin Tetramer II. Time-Resolved Fluorescence Spectroscopy

    E-Print Network [OSTI]

    van Stokkum, Ivo

    Spectroscopic Properties of a Self-Assembled Zinc Porphyrin Tetramer II. Time-Resolved Fluorescence tetramer [Part I], with a 1 ns rotational correlation time at 10 °C. The initial fluorescence anisotropy of the monomer is found to be 0.1. In the tetramer an additional depolarization process occurs with a correlation

  10. Functionalized Europium Oxide Nanoparticles Used as a Fluorescent Label in an Immunoassay

    E-Print Network [OSTI]

    Hammock, Bruce D.

    Functionalized Europium Oxide Nanoparticles Used as a Fluorescent Label in an Immunoassay properties that are typical of europium, that is, a spectrally narrow, red emission and a long fluorescence may be based on lanthanide-derived phosphors. For example, the optical properties of europium chelates

  11. Algorithms for Fluorescence Lifetime Microscopy and Optical Coherence Tomography Data Analysis: Applications for Diagnosis of Atherosclerosis and Oral Cancer

    E-Print Network [OSTI]

    Pande, Paritosh

    2014-05-16T23:59:59.000Z

    imaging (FLIM) with optical coherence tomography (OCT), for the diagnosis of atherosclerosis and oral cancer. FLIM is a fluorescence imaging technique that is capable of providing information about auto fluorescent tissue biomolecules. OCT on the other...

  12. Breaking the Diffraction Barrier in Fluorescence Microscopy by Optical Shelving Stefan Bretschneider, Christian Eggeling, and Stefan W. Hell*

    E-Print Network [OSTI]

    Weeks, Eric R.

    Breaking the Diffraction Barrier in Fluorescence Microscopy by Optical Shelving Stefan the breaking of the diffraction resolution barrier in far-field fluorescence microscopy by transiently shelving barrier by shelving the fluorophore in a metastable dark state, thereby effectively depleting

  13. Electronic starter device for fluorescent lamps. Final report

    SciTech Connect (OSTI)

    NONE

    1998-06-01T23:59:59.000Z

    Due to silicon supplier failures to produce the 03/04 triac silicon as specified in the original proposal, the direction of the starter program was migrated to use available off the shelf power semiconductors. This had unexpected positive side effects including a reduction in component price, improved quality, and the refocus of engineering efforts to concentrate on the Super ASIC core technology. The starter program has begun shipments employing this new architecture, and is being well received both in the US and abroad. In its present form, the starter meets original cost projections within 20%. Work is continuing on the 0.8 micron ASIC, which will allow for the starter to sell below $1.00 in volume. Even at the slightly higher price, interest is strong in replacing the low performance glow starter for small fluorescent applications with a high performance alternative.

  14. Ultraviolet Germicidal Irradiation and Its Effects on Elemental Distributions in Mouse Embryonic Fibroblast Cells in X-Ray Fluorescence Microanalysis

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Jin, Qiaoling; Vogt, Stefan; Lai, Barry; Chen, Si; Finney, Lydia; Gleber, Sophie-Charlotte; Ward, Jesse; Deng, Junjing; Mak, Rachel; Moonier, Nena; et al

    2015-02-23T23:59:59.000Z

    Rapidly-frozen hydrated (cryopreserved) specimens combined with cryo-scanning x-ray fluorescence microscopy provide an ideal approach for investigating elemental distributions in biological cells and tissues. However, because cryopreservation does not deactivate potentially infectious agents associated with Risk Group 2 biological materials, one must be concerned with contamination of expensive and complicated cryogenic x-ray microscopes when working with such materials. We employed ultraviolet germicidal irradiation to decontaminate previously cryopreserved cells under liquid nitrogen, and then investigated its effects on elemental distributions under both frozen hydrated and freeze dried states with xray fluorescence microscopy. We show that the contents and distributions of most biologicallymore »important elements remain nearly unchanged when compared with non-ultravioletirradiated counterparts, even after multiple cycles of ultraviolet germicidal irradiation and cryogenic x-ray imaging. This provides a potential pathway for rendering Risk Group 2 biological materials safe for handling in multiuser cryogenic x-ray microscopes without affecting the fidelity of the results.« less

  15. COMPARING FOREST MANAGEMENT PRACTICES UNDER

    E-Print Network [OSTI]

    COMPARING FOREST MANAGEMENT PRACTICES UNDER COMMUNITY-BASED AND CONVENTIONAL TENURES IN BRITISH OF MASTER OF RESOURCE MANAGEMENT In the School of Resource and Environmental Management © David Mealiea 2011 of Canada, this work may be reproduced, without authorization, under the conditions for Fair Dealing

  16. Blackbody comparator for thermocouple calibration

    SciTech Connect (OSTI)

    Ojanen, M.; Hahtela, O. M.; Heinonen, M. [Centre for Metrology and Accreditation (MIKES), P.O.Box 9, FI-02151 Espoo (Finland)

    2013-09-11T23:59:59.000Z

    MIKES is developing a measurement set-up for calibrating thermocouples in the temperature range 960 °C - 1500 °C. The calibration method is based on direct comparison of thermocouples and radiation thermometers. We have designed a graphite blackbody comparator cell, which is operated in a horizontal single-zone tube furnace. The cell includes two blackbody cavities for radiation temperature measurements. The cavities have openings on opposite sides of the cell, allowing simultaneous measurement with two radiation thermometers. The design of the comparator allows three thermocouples to be calibrated simultaneously. The thermocouples to be calibrated are inserted in thermometer wells around one of the measurement cavities. We characterize the blackbody comparator in terms of repeatability, temperature distribution and emissivity. Finally, we validate the uncertainty analysis by comparing calibration results obtained for type B and S thermocouples to the calibration results reported by Technical Research Institute of Sweden (SP), and MIKES. The agreement in the temperature range 1000 °C - 1500 °C is within 0.90 °C, the average deviation being 0.17 °C.

  17. COMPARATIVE MEDICINE LABORATORY ANIMAL FACILITIES

    E-Print Network [OSTI]

    Krovi, Venkat

    4.A.7 COMPARATIVE MEDICINE LABORATORY ANIMAL FACILITIES STANDARD OPERATING PROCEDURES LUNAR PIXIMUS MACHINE 1.0 Purpose This procedure outlines precautions, maintenance and use of the Lunar PIXImus Machine housed in room 310 BEB. 2.0 Scope This procedure applies to all CMLAF and principal investigator staff. 3

  18. COMPARATIVE MEDICINE LABORATORY ANIMAL FACILITIES

    E-Print Network [OSTI]

    Krovi, Venkat

    3.E.1 COMPARATIVE MEDICINE LABORATORY ANIMAL FACILITIES STANDARD OPERATING PROCEDURES for ACCESS, and the correct way to leave the facility. 2.0 Scope: This procedure applies to all CMLAF staff, maintenance, ENTRY, AND EXIT PROCEDURES FOR THE ANIMAL BIOSAFETY SUITE ROOM 305 BEB 1.0 Purpose: The Biosafety suite

  19. COMPARATIVE MEDICINE LABORATORY ANIMAL FACILITIES

    E-Print Network [OSTI]

    Krovi, Venkat

    5.A.4 COMPARATIVE MEDICINE LABORATORY ANIMAL FACILITIES STANDARD OPERATING PROCEDURE for CRITICAL Plant and maintenance personnel as well as CMLAF personnel that will be notified. 3.0 Procedure ALARM RESPONSE PROCEDURE FOR CHILLED WATER PLANT 1.0 Purpose: This SOP outlines the procedure

  20. COMPARATIVE MEDICINE LABORATORY ANIMAL FACILITIES

    E-Print Network [OSTI]

    Krovi, Venkat

    1.E.1 COMPARATIVE MEDICINE LABORATORY ANIMAL FACILITIES STANDARD OPERATING PROCEDURE for ENTRY RODENT FACILITY 1. I have read, understand, and will follow the Standard Operating Procedures listed: This procedure applies to all CMLAF, principal investigator and maintenance personnel 3.0 Procedure: 3

  1. Fiber-optic fluorescence imaging Benjamin A Flusberg, Eric D Cocker,Wibool Piyawattanametha, Juergen C Jung,

    E-Print Network [OSTI]

    Cai, Long

    Fiber-optic fluorescence imaging Benjamin A Flusberg, Eric D Cocker,Wibool Piyawattanametha, Juergen C Jung, Eunice L M Cheung & Mark J Schnitzer Optical fibers guide light between separate locations and enable new types of fluorescence imaging. Fiber-optic fluorescence imaging systems include portable

  2. Cyanine-based probe\\tag-peptide pair for fluorescence protein imaging and fluorescence protein imaging methods

    DOE Patents [OSTI]

    Mayer-Cumblidge, M. Uljana (Richland, WA); Cao, Haishi (Richland, WA)

    2010-08-17T23:59:59.000Z

    A molecular probe comprises two arsenic atoms and at least one cyanine based moiety. A method of producing a molecular probe includes providing a molecule having a first formula, treating the molecule with HgOAc, and subsequently transmetallizing with AsCl.sub.3. The As is liganded to ethanedithiol to produce a probe having a second formula. A method of labeling a peptide includes providing a peptide comprising a tag sequence and contacting the peptide with a biarsenical molecular probe. A complex is formed comprising the tag sequence and the molecular probe. A method of studying a peptide includes providing a mixture containing a peptide comprising a peptide tag sequence, adding a biarsenical probe to the mixture, and monitoring the fluorescence of the mixture.

  3. Hard X-rays and Fluorescent Iron Emission from the Embedded Infrared Cluster in NGC 2071

    E-Print Network [OSTI]

    Stephen L. Skinner; Audrey E. Simmons; Marc Audard; Manuel Guedel

    2006-12-19T23:59:59.000Z

    We present first results of XMM-Newton X-ray observations of the infrared cluster lying near the NGC 2071 reflection nebula in the Orion B region. This cluster is of interest because it is one of the closest regions known to harbor embedded high-mass stars. We report the discovery of hard X-ray emission from the dense central NGC 2071-IR subgroup which contains at least three high-mass young stellar objects (NGC 2071 IRS-1, IRS-2, and IRS-3). A prominent X-ray source is detected within 1 arcsecond of the infrared source IRS-1, which is thought to drive a powerful bipolar molecular outflow. The X-ray spectrum of this source is quite unusual compared to the optically thin plasma spectra normally observed in young stellar objects (YSOs). The spectrum is characterized by a hard broad-band continuum plus an exceptionally broad emission line at approximately 6.4 keV from neutral or near-neutral iron. The fluorescent Fe line likely originates in cold material near the embedded star (i.e. a disk or envelope) that is irradiated by the hard heavily-absorbed X-ray source.

  4. On-site demonstration procedure for solid-state fluorescent ballast

    SciTech Connect (OSTI)

    Verderber, R.; Morse, O.

    1980-09-01T23:59:59.000Z

    The report was presented to plant engineers and managers who were involved in an on-site demonstration of EETech solid-state ballasts for two 40-watt T12 fluorescent lamps. The report includes a brief review of the operating principles of solid-state fluorescent ballasts and the status of development achieved during the LBL program. The remainder of the test describes the techniques of managing and instrumenting a test area for assessing the performance of solid-state fluorescent ballasts at an occupied site.

  5. Imaging Hydrated Microbial Extracellular Polymers: Comparative...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Hydrated Microbial Extracellular Polymers: Comparative Analysis by Electron Microscopy . Imaging Hydrated Microbial Extracellular Polymers: Comparative Analysis by Electron...

  6. Airborne laser induced fluorescence imaging. Innovative technology summary report

    SciTech Connect (OSTI)

    NONE

    1999-06-01T23:59:59.000Z

    Laser-Induced Fluorescence (LIF) was demonstration as part of the Fernald Environmental Management Project (FEMP) Plant 1 Large Scale Demonstration and Deployment Project (LSDDP) sponsored by the US Department of Energy (DOE) Office of Science and Technology, Deactivation and Decommissioning Focus Area located at the Federal Energy Technology Center (FETC) in Morgantown, West Virginia. The demonstration took place on November 19, 1996. In order to allow the contaminated buildings undergoing deactivation and decommissioning (D and D) to be opened to the atmosphere, radiological surveys of floors, walls and ceilings must take place. After successful completion of the radiological clearance survey, demolition of the building can continue. Currently, this process is performed by collecting and analyzing swipe samples for radiological analysis. Two methods are used to analyze the swipe samples: hand-held frisker and laboratory analysis. For the purpose of this demonstration, the least expensive method, swipe samples analyzed by hand-held frisker, is the baseline technology. The objective of the technology demonstration was to determine if the baseline technology could be replaced using LIF.

  7. Diagnostic Implications of the Reactivity of Fluorescence Tracers

    SciTech Connect (OSTI)

    Sick, V; Westbrook, C

    2008-07-14T23:59:59.000Z

    Measurements of fuel concentration distributions with planar laser induced fluorescence of tracer molecules that are added to a base fuel are commonly used in combustion research and development. It usually is assumed that the tracer concentration follows the parent fuel concentration if physical properties such as those determining evaporation are matched. As an example to address this general issue a computational study of combustion of biacetyl/iso-octane mixtures was performed to investigate how well the concentration of biacetyl represents the concentration of iso-octane. For premixed mixture conditions with flame propagation the spatial concentration profiles of the two species in the flame front are separated by 110 {micro}m at 1 bar and by 11 {micro}m at 10 bar. For practical applications this spatial separation is insignificantly small. However, for conditions that mimic ignition and combustion in diesel and HCCI-like operation the differences in tracer and fuel concentration can be significant, exceeding hundreds of percent. At low initial temperature biacetyl was found to be more stable whereas at higher temperature (>1000K) iso-octane is more stable. Similar findings were obtained for a multi-component fuel comprised of iso-octane, n-heptane, methylcyclohexane, and toluene. It may be assumed that similar differences can exist for other tracer/fuel combinations. Caution has therefore to be applied when interpreting PLIF measurements in homogeneous reaction conditions such as in HCCI engine studies.

  8. Simulation of Nuclear Resonance Fluorescence in Geant4

    SciTech Connect (OSTI)

    Jordan, David V.; Warren, Glen A.

    2007-12-31T23:59:59.000Z

    Abstract– Ongoing research at Pacific Northwest National Laboratory (PNNL) is assessing the utility of exploiting nuclear resonance fluorescence (NRF) to detect bulk samples of special nuclear materials, high explosives, and related illicit cargo constituents in a variety of cargo inspection scenarios. PNNL has developed a Monte Carlo radiation transport simulation capability for studying the NRF signal response in order to facilitate computational studies of these inspection scenarios. The simulation framework implements NRF as a new physics process, “G4NRF,” in the Geant4 modeling toolkit, and leverages the Evaluated Nuclear Structure Data File (ENSDF) database of nuclear level properties to determine isotope-specific parameters of the NRF response model in general cargo constituents. The G4NRF package has been benchmarked against bremsstrahlung photon-beam data collected at the University of California at Santa Barbara’s 5.3 MeV accelerator. A series of cargo-inspection scenario studies utilizing the NRF simulation package is currently in progress.

  9. Portable x-ray fluorescence spectrometer. Innovative technology summary report

    SciTech Connect (OSTI)

    NONE

    1998-12-01T23:59:59.000Z

    This report describes the application of portable X-ray fluorescence (XRF) spectrometry to characterize materials related to deactivation and decommissioning (D and D) of contaminated facilities. Two portable XRF instruments manufactured by TN Spectrace were used in a technology evaluation as part of the Large-Scale Demonstration Project (LSDP) held at the Chicago Pile-5 Research Reactor (CP-5) located at Argonne National Laboratory (ANL). The LSDP is sponsored by the US Department of Energy (DOE), Office of Science and Technology, Deactivation and Decommissioning Focus Are (DDFA). The objective of the LSDP is to demonstrate innovative technologies or technology applications potentially beneficial to the D and D of contaminated facilities. The portable XRF technology offers several potential benefits for rapid characterization of facility components and contaminants, including significant cost reduction, fast turnaround time,a nd virtually no secondary waste. Field work for the demonstration of the portable XRF technology was performed from August 28--September 3, 1996 and October 30--December 13, 1996.

  10. Fluorescence from a quantum dot and metallic nanosphere hybrid system

    SciTech Connect (OSTI)

    Schindel, Daniel G. [Department of Mathematics and Statistics, University of Winnipeg, 515 Portage Avenue, Winnipeg, MB, R3B 2E9 (Canada); Singh, Mahi R. [Department of Physics and Astronomy, University of Western Ontario, 1151 Richmond Street, London, ON, N6A 3K7 (Canada)

    2014-03-31T23:59:59.000Z

    We present energy absorption and interference in a quantum dot-metallic nanosphere system embedded on a dielectric substrate. A control field is applied to induce dipole moments in the nanosphere and the quantum dot, and a probe field is applied to monitor absorption. Dipole moments in the quantum dot or the metal nanosphere are induced, both by the external fields and by each other's dipole fields. Thus, in addition to direct polarization, the metal nanosphere and the quantum dot will sense one another via the dipole-dipole interaction. The density matrix method was used to show that the absorption spectrum can be split from one peak to two peaks by the control field, and this can also be done by placing the metal sphere close to the quantum dot. When the two are extremely close together, a self-interaction in the quantum dot produces an asymmetry in the absorption peaks. In addition, the fluorescence efficiency can be quenched by the addition of a metal nanosphere. This hybrid system could be used to create ultra-fast switching and sensing nanodevices.

  11. Computational Prediction of Absorbance Maxima for a Structurally Diverse Series of Engineered Green Fluorescent Protein Chromophores

    E-Print Network [OSTI]

    Campbell, Robert E.

    Fluorescent Protein Chromophores Qadir K. Timerghazin, Haley J. Carlson, Chen Liang, Robert E. Campbell,* and Alex Brown* UniVersity of Alberta, Department of Chemistry, Edmonton, Alberta T6G 2G2, Canada Recei

  12. A parallel adaptive finite element simplified spherical harmonics approximation solver for frequency domain fluorescence molecular imaging

    E-Print Network [OSTI]

    Virginia Tech

    fluorescence molecular imaging Yujie Lu1 , Banghe Zhu1 , Haiou Shen2 , John C Rasmussen1 , Ge Wang2 and Eva M of the domain at excitation and emission wavelengths xf a absorption coefficient of the fluorophore cb light

  13. Supervised Machine Learning Algorithms for Early Detection of Oral Epithelial Cancer Using Fluorescence Lifetime Imaging Microscopy

    E-Print Network [OSTI]

    Lee, Joohyung

    2014-08-06T23:59:59.000Z

    In this study, the clinical potential of the endogenous multispectral Fluorescence lifetime imaging microscopy (FLIM) was investigated to objectively detect oral cancer. To this end, in vivo FLIM imaging was performed on a hamster cheek pouch model...

  14. The Application of Fluorescence Lifetime Imaging Microscopy to Quantitatively Map Mixing and Temperature in Microfluidic Systems 

    E-Print Network [OSTI]

    Graham, Emmelyn M

    2008-01-01T23:59:59.000Z

    The technique of Fluorescence Lifetime Imaging Microscopy (FLIM) has been employed to quantitatively and spatially map the fluid composition and temperature within microfluidic systems. A molecular probe with a ...

  15. Mechanism of the efficient quenching of tryptophan fluorescence in human gamma crystallin

    E-Print Network [OSTI]

    Chen, Jiejin, Ph. D. Massachusetts Institute of Technology

    2008-01-01T23:59:59.000Z

    Quenching of the fluorescence of buried tryptophans (Trps) is an important reporter of protein conformation. Human [gamma]D-crystallin (H[gamma]D-Crys) and human [gamma]S-crystallin (H[gamma]S-Crys) are both very stable ...

  16. Synthesis and Characterization of Novel Fluorescent Injectable Micro-Carriers for Tissue Regeneration

    E-Print Network [OSTI]

    Arora, Akshi

    2014-04-28T23:59:59.000Z

    Specific problem: Our previous study showed that the nanofibrous poly-l-lactic acid (NF-PLLA) microspheres are excellent cell carriers for tissue regeneration. However, these injectable microspheres are not fluorescent biomaterials. Incorporation...

  17. Optimization of Two-photon Excited Fluorescence Enhancement between Tunable and Broadband Femtosecond Laser Pulse Excitations

    E-Print Network [OSTI]

    Wang, Chao

    2012-02-14T23:59:59.000Z

    This project explores optimization of two-photon excited fluorescence (TPEF) enhancement between tunable narrowband and un-tuned broadband femtosecond (fs) laser pulse excitations for two-photon microscopy (TPM). The research is conducted...

  18. Examining 239Pu and 240Pu Nuclear Resonance Fluorescence Measurements on Spent Fuel for Nuclear Safeguards

    E-Print Network [OSTI]

    Quiter, Brian

    2013-01-01T23:59:59.000Z

    10- 01096) Journal of Nuclear Technology, p. 150, Vol. 175,linac and laser technologies for nuclear photonics gamma-rayNuclear resonance fluorescence (NRF) has been identified as a technology

  19. Non-invasive detection of oral cancer using reflectance and fluorescence spectroscopy

    E-Print Network [OSTI]

    McGee, Sasha Alanda

    2008-01-01T23:59:59.000Z

    In vivo reflectance and fluorescence spectra were collected from patients with oral lesions, as well as healthy volunteers, in order to evaluate the potential of spectroscopy to serve as a non-invasive tool for the detection ...

  20. Quantitative microscopic spectral fluorescence measurement of crude oil, bitumen, kerogen, and coal

    SciTech Connect (OSTI)

    Mukhopadhyay, P.K.; Rullkoetter, J.

    1986-05-01T23:59:59.000Z

    Ten samples each of black shale (kerogen and bitumen fractions) from Lias epsilon, coal from Western Canada and nine crude oil and condensate samples from Alaska and northern Germany have been studied using quantitative microscopic spectral fluorescence. The parameters used are lambda/sub max/, red/green quotient (Q), and alteration of fluorescence emission intensity under UV excitation. Using the same parameters, the data show that kerogen and crude oil have opposite maturation trends. Autochthonous bitumens include both kerogen and crude oil characters. Immature, biodegraded, or normal crude oil of different maturity can be characterized using these parameters. Quantitative spectral fluorescence microscopy yields more accurate maturation parameters for the Type I and II kerogens than vitrinite reflectance because the fluorescence of liptinites are used (i.e., the main oil-generating macerals). This method may become the most suitable inexpensive scanning technique for the characterization of crude oil, condensate, and autochthonous/allochthonous source rock bitumens.

  1. Measurement and model assessment of fluorescence lifetime sensing in multiply scattering media

    E-Print Network [OSTI]

    Kuwana, Eddy

    2005-08-29T23:59:59.000Z

    fluorescent dyes, 3,3-diethylthiatricarbocyanine iodide (DTTCI) and Indocynanine Green (ICG), which exhibit distinctly different lifetimes and each exhibits single-exponential decay kinetics, were employed. Measurements of phase-modulation as a function...

  2. LED-Induced Fluorescence System for Tea Classification and Quality Assessment

    E-Print Network [OSTI]

    Dong, Yongjiang; Mei, Liang; Feng, Chao; Yan, Chunsheng; He, Sailing

    2013-01-01T23:59:59.000Z

    A fluorescence system is developed by using several light emitting diodes (LEDs) with different wavelengths as excitation light sources. The fluorescence detection head consists of multi LED light sources and a multimode fiber for fluorescence collection, where the LEDs and the corresponding filters can be easily chosen to get appropriate excitation wavelengths for different applications. By analyzing fluorescence spectra with the principal component analysis method, the system is utilized in the classification of four types of green tea beverages and two types of black tea beverages. Qualities of the Xihu Longjing tea leaves of different grades, as well as the corresponding liquid tea samples, are studied to further investigate the ability and application of the system in the evaluation of classification/quality of tea and other foods.

  3. An improved understanding of fluorescent Zn(II) sensors and their uses in biological settings

    E-Print Network [OSTI]

    Wong, Brian Alexander

    2009-01-01T23:59:59.000Z

    Chapter 1. An Introduction to Fluorescent Zn(II) Sensors and Their Applications in Biological Systems This chapter opens with an overview of the numerous roles of zinc in biology, with an emphasis on labile Zn(II), that ...

  4. Turn-on fluorescent probes for detecting nitric oxide in biology

    E-Print Network [OSTI]

    McQuade, Lindsey Elizabeth, 1981-

    2010-01-01T23:59:59.000Z

    Chapter 1. Investigating the Biological Roles of Nitric Oxide and Other Reactive Nitrogen Species Using Fluorescent Probes: This chapter presents an overview of recent progress in the field of reactive nitrogen species ...

  5. Computational image analysis of subcellular dynamics in time-lapse fluorescence microscopy

    E-Print Network [OSTI]

    Huang, Austin V., 1980-

    2005-01-01T23:59:59.000Z

    The use of image segmentation and motion tracking algorithms was adapted for analyzing time-lapse data of cells with fluorescently labeled protein. Performance metrics were devised and algorithm parameters were matched to ...

  6. Time resolved fluorescence of CdSe nanocrystals using single molecule spectroscopy

    E-Print Network [OSTI]

    Fisher, Brent R

    2005-01-01T23:59:59.000Z

    A wide variety of spectroscopic studies of CdSe nanocrystals (NCs) are presented in this thesis, all studying some aspect of the temporal evolution of NC fluorescence tinder different conditions. In particular the methods ...

  7. Stardust Interstellar Preliminary Examination VI: Quantitative elemental analysis by synchrotron X-ray fluorescence nanoimaging of eight impact features in aerogel

    E-Print Network [OSTI]

    Nittler, Larry R.

    -ray fluorescence nanoimaging of eight impact features in aerogel Alexandre S. SIMIONOVICI1* , Laurence LEMELLE2

  8. Comparative analysis of collaboration networks

    SciTech Connect (OSTI)

    Progulova, Tatiana; Gadjiev, Bahruz [International University for Nature, Society and Man, 19 Universitetskaya Street, Dubna, 141980 (Russian Federation)

    2011-03-14T23:59:59.000Z

    In this paper we carry out a comparative analysis of the word network as the collaboration network based on the novel by M. Bulgakov 'Master and Margarita', the synonym network of the Russian language as well as the Russian movie actor network. We have constructed one-mode projections of these networks, defined degree distributions for them and have calculated main characteristics. In the paper a generation algorithm of collaboration networks has been offered which allows one to generate networks statistically equivalent to the studied ones. It lets us reveal a structural correlation between word network, synonym network and movie actor network. We show that the degree distributions of all analyzable networks are described by the distribution of q-type.

  9. Automation of the Laguerre Expansion Technique for Analysis of Time-resolved Fluorescence Spectroscopy Data

    E-Print Network [OSTI]

    Dabir, Aditi Sandeep

    2010-07-14T23:59:59.000Z

    AUTOMATION OF THE LAGUERRE EXPANSION TECHNIQUE FOR ANALYSIS OF TIME-RESOLVED FLUORESCENCE SPECTROSCOPY DATA A Thesis by ADITI SANDEEP DABIR Submitted to the Office of Graduate Studies of Texas A&M University in partial... fulfillment of the requirements for the degree of MASTER OF SCIENCE December 2009 Major Subject: Biomedical Engineering AUTOMATION OF THE LAGUERRE EXPANSION TECHNIQUE FOR ANALYSIS OF TIME-RESOLVED FLUORESCENCE SPECTROSCOPY DATA A Thesis...

  10. On-line measurement of lignin in wood pulp by color shift of fluorescence

    DOE Patents [OSTI]

    Jeffers, L.A.; Malito, M.L.

    1996-01-23T23:59:59.000Z

    Lignin concentrations from wood pulp samples are measured by applying an excitation light at a selected wavelength to the samples in order to cause the lignin to emit fluorescence. A spectral distribution of the fluorescence emission is then determined. The lignin concentration is then calculated based on the spectral distribution signal. The spectral distribution is quantified by either a wavelength centroid method or a band ratio method. 6 figs.

  11. On-line measurement of lignin in wood pulp by color shift of fluorescence

    DOE Patents [OSTI]

    Jeffers, Larry A. (Washington Township, Stark County, OH); Malito, Michael L. (Liberty Township, Trumbull County, OH)

    1996-01-01T23:59:59.000Z

    Lignin concentrations from wood pulp samples are measured by applying an excitation light at a selected wavelength to the samples in order to cause the lignin to emit fluorescence. A spectral distribution of the fluorescence emission is then determined. The lignin concentration is then calculated based on the spectral distribution signal. The spectral distribution is quantified by either a wavelength centroid method or a band ratio method.

  12. Analyzing fluorophore electronic structure and depolarization by fluorescence polarizing angle spectrum

    SciTech Connect (OSTI)

    Mu, Taotao; Chen, Siying, E-mail: csy@bit.edu.cn; Zhang, Yinchao; Chen, He; Guo, Pan [School of Optoelectronics, Beijing Institute of Technology, Beijing 100081 (China)

    2014-07-21T23:59:59.000Z

    In this Letter, a method, based on stokes parameters, is developed to observe the angular displacement between the excitation and emission moments. Experiments demonstrate that when combined with degree of polarization spectrums, we can acquire the depolarization caused by angular displacement or energy migration. The method presented in this Letter can be easily realized with the existing fluorescence measuring system and may potentially make it convenient to study the fluorophore electronic structure or the mechanism of fluorescence anisotropy.

  13. The development of an indirect fluorescent antibody test for Trypanosoma vivax in Colombia

    E-Print Network [OSTI]

    Platt, Kenneth Bradley

    1974-01-01T23:59:59.000Z

    THE DEVELOPMENT OF AN INDIRECT FLUORESCENT ANTIBODY TEST FOR TRYPANOSOMA VIVAX IN COLOMBIA A Thesis KENNETH BRADLEY PLATT Submitted to the Graduate College of Texas A Es M University in partial fulfillment of the requirement for the degree... of MASTEB OF SCIENCE May 1974 Ma]or Sub]ect: Veterinary Miorobiology THE DEVELOPMENT OF AN INDIRECT FLUORESCENT ANTIBODY TEST FOR TRYPANOSOMA VIVAX IN COLOMBIA A Thesis by KENNETH BRADLEY PLATT Approved as to style end content by: Co-Cha rman...

  14. Organic light-emitting device with a phosphor-sensitized fluorescent emission layer

    DOE Patents [OSTI]

    Forrest, Stephen (Ann Arbor, MI); Kanno, Hiroshi (Osaka, JP)

    2009-08-25T23:59:59.000Z

    The present invention relates to organic light emitting devices (OLEDs), and more specifically to OLEDS that emit light using a combination of fluorescent emitters and phosphorescent emitters. The emissive region of the devices of the present invention comprise at least one phosphor-sensitized layer which has a combined emission from a phosphorescent emitter and a fluorescent emitter. In preferred embodiments, the invention relates to white-emitting OLEDS (WOLEDs).

  15. Assessment of Nuclear Resonance Fluorescence for Spent Nuclear Fuel Assay

    SciTech Connect (OSTI)

    Quiter, Brian; Ludewigt, Bernhard; Ambers, Scott

    2011-06-30T23:59:59.000Z

    In nuclear resonance fluorescence (NRF) measurements, resonances are excited by an external photon beam leading to the emission of gamma rays with specific energies that are characteristic of the emitting isotope. NRF promises the unique capability of directly quantifying a specific isotope without the need for unfolding the combined responses of several fissile isotopes as is required in other measurement techniques. We have analyzed the potential of NRF as a non-destructive analysis technique for quantitative measurements of Pu isotopes in spent nuclear fuel (SNF). Given the low concentrations of 239Pu in SNF and its small integrated NRF cross sections, the main challenge in achieving precise and accurate measurements lies in accruing sufficient counting statistics in a reasonable measurement time. Using analytical modeling, and simulations with the radiation transport code MCNPX that has been experimentally tested recently, the backscatter and transmission methods were quantitatively studied for differing photon sources and radiation detector types. Resonant photon count rates and measurement times were estimated for a range of photon source and detection parameters, which were used to determine photon source and gamma-ray detector requirements. The results indicate that systems based on a bremsstrahlung source and present detector technology are not practical for high-precision measurements of 239Pu in SNF. Measurements that achieve the desired uncertainties within hour-long measurements will either require stronger resonances, which may be expressed by other Pu isotopes, or require quasi-monoenergetic photon sources with intensities that are approximately two orders of magnitude higher than those currently being designed or proposed.This work is part of a larger effort sponsored by the Next Generation Safeguards Initiative to develop an integrated instrument, comprised of individual NDA techniques with complementary features, that is fully capable of determining Pu mass in spent fuel assemblies.

  16. Conservation potential of compact fluorescent lamps in India and Brazil

    SciTech Connect (OSTI)

    Gadgil, A.; Martino Jannuzzi, G. de (Lawrence Berkeley Lab., CA (USA); Universidade Estadual de Campinas, SP (Brazil). Faculdade de Engenharia)

    1989-07-01T23:59:59.000Z

    We evaluate the conservation potential of compact fluorescent lamps (CFLs) for managing the rapidly increasing electrical energy and peak demand in India and Brazil. Using very conservative assumptions, we find that the cost of conserved energy using 16 W CFLs is 4 and 6 times less than the long range marginal cost of electricity for the two countries. The cost of avoided peak installed capacity is 6 and 9.5 times less than the cost of new installed capacity for India and Brazil. The analysis is undertaken from the three separate perspectives of the national economies, the consumers, and the utilities. We find that because residential electricity is subsidized, the consumers have little or no incentive to purchase and install the CFLs, unless they too are subsidized. However, the benefits of CFL installation to the utility are so large that subsidizing them is a paying proposition for the utility are so large that subsidizing them is a paying proposition for the utility in almost all cases. As an illustration of a gradual introduction strategy for CFLs, we calculate a scenario where national savings of the order of US $1.2 million per day for India and US $2.5 million per day for Brazil are reached in 10 years by a small and gradual transfer of subsidy from residential electricity to CFLs. We then explore the barriers to immediate large scale introduction of these lamps in the two countries. Specific technical and marketing problems are identified and discussed, which would require solution before such an introduction can be attempted. Lastly, we discuss the range of policy instruments, in addition to a subsidy scheme, that can be used for promoting the diffusion of these lamps in the domestic and commercial sector. 47 refs., 15 figs., 2 tabs.

  17. atomic fluorescence spectroscopy: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    with both trans and gauche conformations bound to the gold clusters at top, bridge and hollow bonding sites. Comparing our results with the experimental data of Hihath et al....

  18. Two photon absorption laser induced fluorescence measurements of neutral density in a helicon plasma

    SciTech Connect (OSTI)

    Galante, M. E.; Magee, R. M.; Scime, E. E. [Department of Physics, West Virginia University, Morgantown, West Virginia 26506 (United States)] [Department of Physics, West Virginia University, Morgantown, West Virginia 26506 (United States)

    2014-05-15T23:59:59.000Z

    We have developed a new diagnostic based on two-photon absorption laser induced fluorescence (TALIF). We use a high intensity (5?MW/cm{sup 2}), narrow bandwidth (0.1?cm{sup ?1}) laser to probe the ground state of neutral hydrogen, deuterium and krypton with spatial resolution better than 0.2?cm, a time resolution of 10?ns, and a measurement cadence of 20?Hz. Here, we describe proof-of-principle measurements in a helicon plasma source that demonstrate the TALIF diagnostic is capable of measuring neutral densities spanning four orders of magnitude; comparable to the edge neutral gradients predicted in the DIII-D tokamak pedestal. The measurements are performed in hydrogen and deuterium plasmas and absolute calibration is accomplished through TALIF measurements in neutral krypton. The optical configuration employed is confocal, i.e., both light injection and collection are accomplished with a single lens through a single optical port in the vacuum vessel. The wavelength resolution of the diagnostic is sufficient to separate hydrogen and deuterium spectra and we present measurements from mixed hydrogen and deuterium plasmas that demonstrate isotopic abundance measurements are feasible. Time resolved measurements also allow us to explore the evolution of the neutral hydrogen density and temperature and effects of wall recycling. We find that the atomic neutral density grows rapidly at the initiation of the discharge, reaching the steady-state value within 1?ms. Additionally, we find that neutral hydrogen atoms are born with 0.08?eV temperatures, not 2?eV as is typically assumed.

  19. Hard X-ray Fluorescence Measurements of Heteroepitaxial Solid Oxide Fuel Cell Cathode Materials

    SciTech Connect (OSTI)

    Davis, Jacob N.; Miara, Lincoln J.; Saraf, Laxmikant V.; Kaspar, Tiffany C.; Gopalan, Srikanth; Pal, Uday B.; Woicik, Joseph C.; Basu, Soumendra N.; Ludwig, Karl F.

    2012-12-01T23:59:59.000Z

    Commonly, SOFCs are operated at high temperatures (above 800°C). At these temperatures expensive housing is needed to contain an operating stack as well as coatings to contain the oxidation of the metallic interconnects. Lowering the temperature of an operating device would allow for more conventional materials to be used, thus lowering overall cost. Understanding the surface chemical states of cations in the surface of the SOFC cathode is vital to designing a system that will perform well at lower temperatures. The samples studied were grown by pulsed laser deposition (PLD) at the Environmental Molecular Sciences Laboratory (EMSL) at Pacific Northwest National Laboratory (PNNL). 20% strontium doped lanthanum manganite (LSM-20) was grown on YSZ and NGO (neodymium gallate). The films on YSZ have a fiber texture. LSM-20 on NGO is heteroepitaxial. Lanthanum strontium cobalt ferrite (LSCF-6428) films were grown on LAO and YSZ with a GDC barrier layer. Total X-ray Reflection Fluorescence (TXRF) was used to depth profile the samples. In a typical experiment, the angle of the incident beam is varied though the critical angle. Below the critical angle, the x-ray decays as an evanescent wave and will only penetrate the top few nanometers. TXRF experiments done on LSM films have suggested strontium segregates to the surface and form strontium enriched nanoparticles (1). It should be pointed out that past studies have focused on 30% strontium A-site doping, but this project uses 20% strontium doped lanthanum manganite. XANES and EXAFS data were taken as a function of incoming angle to probe composition as a function of depth. XANES spectra can be difficult to analyze fully. For other materials density functional theory calculations compared to near edge measurements have been a good way to understand the 3d valence electrons (2).

  20. Comparative Analysis of Proteome and Transcriptome Variation...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Comparative Analysis of Proteome and Transcriptome Variation in Mouse. Comparative Analysis of Proteome and Transcriptome Variation in Mouse. Abstract: The relationships between...

  1. Cetane Performance and Chemistry Comparing Conventional Fuels...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Performance and Chemistry Comparing Conventional Fuels and Fuels Derived from Heavy Crude Sources Cetane Performance and Chemistry Comparing Conventional Fuels and Fuels...

  2. VISTA - computational tools for comparative genomics

    E-Print Network [OSTI]

    Frazer, Kelly A.; Pachter, Lior; Poliakov, Alexander; Rubin, Edward M.; Dubchak, Inna

    2004-01-01T23:59:59.000Z

    tools for comparative genomics Kelly A. Frazer 1 , LiorBerkeley, CA, 94720 Genomics Division, Lawrence Berkeleymultiple comparative genomics tools and provides users with

  3. X-Ray Fluorescence (XRF) Analysis of Obsidian Artifacts from AZ AA:8:20 (ASM), Southeastern Arizona

    E-Print Network [OSTI]

    Shackley, M. Steven

    1986-01-01T23:59:59.000Z

    Minerologist 62:426-437. SOUTHWEST XRF PAPER Tabl;o:. X-:'~vX-RAY FLUORESCENCE (XRF) ANALYSIS OF OBSIDIAN ARTIFACTS FROM

  4. Calculating the contribution of different binding modes to Quinacrine - DNA complex formation from polarized fluorescence data

    E-Print Network [OSTI]

    Voloshin, Igor; Karachevtsev, Victor; Zozulya, Victor

    2013-01-01T23:59:59.000Z

    Binding of acridine derivative quinacrine (QA) to chicken erythrocyte DNA was studied by methods of absorption and polarized fluorescent spectroscopy. Measurements were carried out in aqueous buffered solutions (pH 6.9) of different dye concentrations (QA concentration range from $10^{-6}$ till $10^{-4}$ M) and ionic strengths ($Na^{+}$ concentration rang from $10^{-3}$ till 0.15 M) in a wide range of phosphate-to-dye molar ratios ($P/D$). It is established that the minimum of fluorescent titration curve plotted as relative fluorescence intensity $vs$ $P/D$ is conditioned by the competition between the two types of QA binding to DNA which posses by different emission parameters: (i) intercalative one dominating under high $P/D$ values, and (ii) outside electrostatic binding dominating under low $P/D$ values, which is accompanied by the formation of non-fluorescent dye associates on the DNA backbone. Absorption and fluorescent characteristics of complexes formed were determined. The method of calculation of di...

  5. Optimized scalable stack of fluorescent solar concentrator systems with bifacial silicon solar cells

    SciTech Connect (OSTI)

    Martínez Díez, Ana Luisa, E-mail: a.martinez@itma.es [Fundación ITMA, Parque Empresarial Principado de Asturias, C/Calafates, Parcela L-3.4, 33417 Avilés (Spain); Fraunhofer Institute for Solar Energy Systems ISE, Heidenhofstr. 2, 79110 Freiburg (Germany); Gutmann, Johannes; Posdziech, Janina; Rist, Tim; Goldschmidt, Jan Christoph [Fraunhofer Institute for Solar Energy Systems ISE, Heidenhofstr. 2, 79110 Freiburg (Germany); Plaza, David Gómez [Fundación ITMA, Parque Empresarial Principado de Asturias, C/Calafates, Parcela L-3.4, 33417 Avilés (Spain)

    2014-10-21T23:59:59.000Z

    In this paper, we present a concentrator system based on a stack of fluorescent concentrators (FCs) and a bifacial solar cell. Coupling bifacial solar cells to a stack of FCs increases the performance of the system and preserves its efficiency when scaled. We used an approach to optimize a fluorescent solar concentrator system design based on a stack of multiple fluorescent concentrators (FC). Seven individual fluorescent collectors (20 mm×20 mm×2 mm) were realized by in-situ polymerization and optically characterized in regard to their ability to guide light to the edges. Then, an optimization procedure based on the experimental data of the individual FCs was carried out to determine the stack configuration that maximizes the total number of photons leaving edges. Finally, two fluorescent concentrator systems were realized by attaching bifacial silicon solar cells to the optimized FC stacks: a conventional system, where FC were attached to one side of the solar cell as a reference, and the proposed bifacial configuration. It was found that for the same overall FC area, the bifacial configuration increases the short-circuit current by a factor of 2.2, which is also in agreement with theoretical considerations.

  6. Method for detecting point mutations in DNA utilizing fluorescence energy transfer

    DOE Patents [OSTI]

    Parkhurst, Lawrence J. (Lincoln, NE); Parkhurst, Kay M. (Lincoln, NE); Middendorf, Lyle (Lincoln, NE)

    2001-01-01T23:59:59.000Z

    A method for detecting point mutations in DNA using a fluorescently labeled oligomeric probe and Forster resonance energy transfer (FRET) is disclosed. The selected probe is initially labeled at each end with a fluorescence dye, which act together as a donor/acceptor pair for FRET. The fluorescence emission from the dyes changes dramatically from the duplex stage, wherein the probe is hybridized to the complementary strand of DNA, to the single strand stage, when the probe is melted to become detached from the DNA. The change in fluorescence is caused by the dyes coming into closer proximity after melting occurs and the probe becomes detached from the DNA strand. The change in fluorescence emission as a function of temperature is used to calculate the melting temperature of the complex or T.sub.m. In the case where there is a base mismatch between the probe and the DNA strand, indicating a point mutation, the T.sub.m has been found to be significantly lower than the T.sub.m for a perfectly match probelstand duplex. The present invention allows for the detection of the existence and magnitude of T.sub.m, which allows for the quick and accurate detection of a point mutation in the DNA strand and, in some applications, the determination of the approximate location of the mutation within the sequence.

  7. Subunits of highly Fluorescent Protein R-Phycoerythrin as Probes for Cell Imaging and Single-Molecule Detection

    SciTech Connect (OSTI)

    Dragan Isailovic

    2005-12-17T23:59:59.000Z

    The purposes of our research were: (1) To characterize subunits of highly fluorescent protein R-Phycoerythrin (R-PE) and check their suitability for single-molecule detection (SMD) and cell imaging, (2) To extend the use of R-PE subunits through design of similar proteins that will be used as probes for microscopy and spectral imaging in a single cell, and (3) To demonstrate a high-throughput spectral imaging method that will rival spectral flow cytometry in the analysis of individual cells. We first demonstrated that R-PE subunits have spectroscopic and structural characteristics that make them suitable for SMD. Subunits were isolated from R-PE by high-performance liquid chromatography (HPLC) and detected as single molecules by total internal reflection fluorescence microscopy (TIRFM). In addition, R-PE subunits and their enzymatic digests were characterized by several separation and detection methods including HPLC, capillary electrophoresis, sodium dodecyl sulfate-polyacrilamide gel electrophoresis (SDS-PAGE) and HPLC-electrospray ionization mass spectrometry (ESI-MS). Favorable absorption and fluorescence of the R-PE subunits and digest peptides originate from phycoerythrobilin (PEB) and phycourobilin (PUB) chromophores that are covalently attached to cysteine residues. High absorption coefficients and strong fluorescence (even under denaturing conditions), broad excitation and emission fluorescence spectra in the visible region of electromagnetic spectrum, and relatively low molecular weights make these molecules suitable for use as fluorescence labels of biomolecules and cells. We further designed fluorescent proteins both in vitro and in vivo (in Escherichia coli) based on the highly specific attachment of PEB chromophore to genetically expressed apo-subunits of R-PE. In one example, apo-alpha and apo-beta R-PE subunits were cloned from red algae Polisiphonia boldii (P. boldii), and expressed in E. coli. Although expressed apo-subunits formed inclusion bodies, fluorescent holo-subunits were formed after incubation of E. coli cells with PEB. Spectroscopic characterization of holo-subunits confirmed that the attachment of PEB chromophore to apo-subunits yielded holo-subunits containing both PEB and urobilin (UB). Fluorescence and differential interference contrast (DIC) microscopy showed polar location of holo-subunit inclusion bodies in E. coli cells. In another example, R-PE apo-subunits were genetically fused to cytoplasmic and periplasmic versions of E. coli maltose binding protein (MBP). Fluorescent proteins formed after attachment of PEB to MBP-subunit fusions in vitro and in vivo contained PEB as the sole chromophore, were soluble, and displayed high orange fluorescence. Fluorescence microscopy showed that fusions are located either throughout cells or at cell poles. In addition, cells containing fluorescent holo-subunits or MBP-subunit fusions were up to ten times brighter than control cells as measured by flow cytometry. Results show that the fluorescent proteins formed after non-enzymatic attachment of PEB to R-PE subunit fusions could be used as reporters of gene expression and protein localization in cells as well as fluorescence labels in flow cytometry. Finally, we demonstrated a high-throughput method able to record emission fluorescence spectra of individual cells containing fluorescent proteins. Upon excitation with a 488 mn argon-ion laser many bacterial cells were imaged by a 20X microscope objective while they moved through a capillary tube. Fluorescence was dispersed by a transmission diffraction grating, and an intensified charge-coupled device (ICCD) camera simultaneously recorded the zero and the first orders of the fluorescence from each cell. Single-cell fluorescence spectra were reconstructed from the distance between zero-order and first-order maxima as well as the length and the pixel intensity distribution of the first-order images. By using this approach, the emission spectrum of E. coli cells expressing green fluorescent protein (GFP) was reconstructed. Also, fluorescence spectra of E

  8. Demonstration of x-ray fluorescence imaging of a high-energy-density plasma

    SciTech Connect (OSTI)

    MacDonald, M. J., E-mail: macdonm@umich.edu; Gamboa, E. J. [Department of Atmospheric, Oceanic, and Space Sciences, University of Michigan, Ann Arbor, Michigan 48109 (United States); SLAC National Accelerator Laboratory, Menlo Park, California 94025 (United States); Keiter, P. A.; Fein, J. R.; Klein, S. R.; Kuranz, C. C.; LeFevre, H. J.; Manuel, M. J.-E.; Wan, W. C.; Drake, R. P. [Department of Atmospheric, Oceanic, and Space Sciences, University of Michigan, Ann Arbor, Michigan 48109 (United States); Montgomery, D. S. [Los Alamos National Laboratory, Los Alamos, New Mexico 87545 (United States); Biener, M. M.; Fournier, K. B. [Lawrence Livermore National Laboratory, Livermore, California 94550 (United States); Streit, J. [Schafer Corporation, Livermore, California 94551 (United States)

    2014-11-15T23:59:59.000Z

    Experiments at the Trident Laser Facility have successfully demonstrated the use of x-ray fluorescence imaging (XRFI) to diagnose shocked carbonized resorcinol formaldehyde (CRF) foams doped with Ti. One laser beam created a shock wave in the doped foam. A second laser beam produced a flux of vanadium He-? x-rays, which in turn induced Ti K-shell fluorescence within the foam. Spectrally resolved 1D imaging of the x-ray fluorescence provided shock location and compression measurements. Additionally, experiments using a collimator demonstrated that one can probe specific regions within a target. These results show that XRFI is a capable alternative to path-integrated measurements for diagnosing hydrodynamic experiments at high energy density.

  9. Fluorescence measurements for evaluating the application of multivariate analysis techniques to optically thick environments.

    SciTech Connect (OSTI)

    Reichardt, Thomas A.; Timlin, Jerilyn Ann; Jones, Howland D. T.; Sickafoose, Shane M.; Schmitt, Randal L.

    2010-09-01T23:59:59.000Z

    Laser-induced fluorescence measurements of cuvette-contained laser dye mixtures are made for evaluation of multivariate analysis techniques to optically thick environments. Nine mixtures of Coumarin 500 and Rhodamine 610 are analyzed, as well as the pure dyes. For each sample, the cuvette is positioned on a two-axis translation stage to allow the interrogation at different spatial locations, allowing the examination of both primary (absorption of the laser light) and secondary (absorption of the fluorescence) inner filter effects. In addition to these expected inner filter effects, we find evidence that a portion of the absorbed fluorescence is re-emitted. A total of 688 spectra are acquired for the evaluation of multivariate analysis approaches to account for nonlinear effects.

  10. Standard practice for fluorescent liquid penetrant testing using the Solvent-Removable process

    E-Print Network [OSTI]

    American Society for Testing and Materials. Philadelphia

    2010-01-01T23:59:59.000Z

    1.1 This practice covers procedures for fluorescent penetrant examination utilizing the solvent-removable process. It is a nondestructive testing method for detecting discontinuities that are open to the surface, such as cracks, seams, laps, cold shuts, laminations, isolated porosity, through leaks, or lack of fusion and is applicable to in-process, final, and maintenance examination. It can be effectively used in the examination of nonporous, metallic materials, both ferrous and nonferrous, and of nonmetallic materials such as glazed or fully densified ceramics and certain nonporous plastics and glass. 1.2 This practice also provides a reference: 1.2.1 By which a fluorescent penetrant examination solvent-removable process recommended or required by individual organizations can be reviewed to ascertain its applicability and completeness. 1.2.2 For use in the preparation of process specifications dealing with the fluorescent solvent-removable liquid penetrant examination of materials and parts. Agreement by...

  11. Standard practice for fluorescent liquid penetrant testing using the hydrophilic Post-Emulsification process

    E-Print Network [OSTI]

    American Society for Testing and Materials. Philadelphia

    2010-01-01T23:59:59.000Z

    1.1 This practice covers procedures for fluorescent penetrant examination utilizing the hydrophilic post-emulsification process. It is a nondestructive testing method for detecting discontinuities that are open to the surface such as cracks, seams, laps, cold shuts, laminations, isolated porosity, through leaks, or lack of fusion and is applicable to in-process, final, and maintenance examination. It can be effectively used in the examination of nonporous, metallic materials, both ferrous and nonferrous, and of nonmetallic materials such as glazed or fully densified ceramics and certain nonporous plastics and glass. 1.2 This practice also provides a reference: 1.2.1 By which a fluorescent penetrant examination hydrophilic post-emulsification process recommended or required by individual organizations can be reviewed to ascertain their applicability and completeness. 1.2.2 For use in the preparation of process specifications dealing with the fluorescent penetrant examination of materials and parts using the...

  12. Hyper-filter-fluorescer spectrometer for x-rays above 120 keV

    DOE Patents [OSTI]

    Wang, Ching L. (Livermore, CA)

    1983-01-01T23:59:59.000Z

    An apparatus utilizing filter-fluorescer combinations is provided to measure short bursts of high fluence x-rays above 120 keV energy, where there are no practical absorption edges available for conventional filter-fluorescer techniques. The absorption edge of the prefilter is chosen to be less than that of the fluorescer, i.e., E.sub.PRF E.sub.F. In this way, the response function is virtually zero between E.sub.PRF and E.sub.F and well defined and enhanced in an energy band of less than 1000 keV above the 120 keV energy.

  13. Intense Internal and External Fluorescence as Solar Cells Approach the Shockley-Queisser Efficiency Limit

    E-Print Network [OSTI]

    Miller, Owen D; Kurtz, Sarah R

    2012-01-01T23:59:59.000Z

    Absorbed sunlight in a solar cell produces electrons and holes. But, at the open circuit condition, the carriers have no place to go. They build up in density and, ideally, they emit external fluorescence that exactly balances the incoming sunlight. Any additional non-radiative recombination impairs the carrier density buildup, limiting the open-circuit voltage. At open-circuit, efficient external fluorescence is an indicator of low internal optical losses. Thus efficient external fluorescence is, counter-intuitively, a necessity for approaching the Shockley-Queisser efficiency limit. A great Solar Cell also needs to be a great Light Emitting Diode. Owing to the narrow escape cone for light, efficient external emission requires repeated attempts, and demands an internal luminescence efficiency >>90%.

  14. Laser-induced fluorescence of fused silica irradiated by ArF excimer laser

    SciTech Connect (OSTI)

    Zhang Haibo [Shanghai Key Laboratory of All Solid-state Laser and Applied Techniques, Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Science, Shanghai 201800 (China); Graduate University of Chinese Academy of Science, Beijing 100049 (China); Yuan Zhijun; Zhou Jun; Dong Jingxing; Wei Yunrong; Lou Qihong [Shanghai Key Laboratory of All Solid-state Laser and Applied Techniques, Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Science, Shanghai 201800 (China)

    2011-07-01T23:59:59.000Z

    Laser-induced fluorescence (LIF) of high-purity fused silica irradiated by ArF excimer laser is studied experimentally. LIF bands of the fused silica centered at 281 nm, 478 nm, and 650 nm are observed simultaneously. Furthermore, the angular distribution of the three fluorescence peaks is examined. Microscopic image of the laser modified fused silica indicates that scattering of the generated fluorescence by laser-induced damage sites is the main reason for the angular distribution of LIF signals. Finally, the dependence of LIF signals intensities of the fused silica on laser power densities is presented. LIF signals show a squared power density dependence, which indicates that laser-induced defects are formed mainly via two-photon absorption processes.

  15. New maturity indicators based on spectral fluorescence of alginite and bitumen, Monterey Formation, California

    SciTech Connect (OSTI)

    Lee, Chungi; Kennicutt, M.C. II (Texas A and M Univ., College Station (United States)); Lo, H.B. (Exxon Production Research Co., Houston, TX (United States))

    1991-03-01T23:59:59.000Z

    Conventional assessment of maturation level in the Monterey has been problematic, since sporinite and vitrinite are rare or absent. Organic matter is largely alginite and amorphous material, and reliable vitrinite reflectance (R{sub o}%) and Thermal Alteration Index (TAI) are difficult to obtain. Large amounts of bitumen often imbedded in the highly fractured Monterey shales cause a suppression of T{sub max} and low values of S{sub 1}S{sub 1} + S{sub 2}. It is often difficult to determine whether bitumen is indigenous or migrated from other more mature strata. Spectral fluorescence measurements of alginite and bitumen have proved useful in assessing the maturity of the Monterey. A maturity scale based on red/green quotient (Q{sub v}) measured as the fluorescence of alginite B when excited by violet-light has been developed and applied to the Monterey. Alginite B is common in the Monterey, and accurate fluorescent measurements can be readily obtained given the highly fluorescent character of alginite B. A total scanning fluorescence technique was used to develop a maturity scale based on bitumen aromatic content and composition. The maturity parameter (R{sub 1}) developed in this study uses the intensity of fluorescence emitted at 360 nm ratioed to that at 320 nm when the solvent-dissolved bitumen is excited at the 270 nm. These parameters allow for the evaluation of the thermal maturity of algal organic matter and bitumen from the Monterey with R{sub o}% {lt} 1. Indigenous bitumen is also indicated by a comparison of maturity based on Q{sub v} (the solid phase) and bitumen maturity (the liquid phase) based on R{sub 1}.

  16. Simultaneous two-dimensional laser-induced-fluorescence measurements of argon ions

    SciTech Connect (OSTI)

    Hansen, A. K.; Galante, Matthew; McCarren, Dustin; Sears, Stephanie; Scime, E. E. [Department of Physics, West Virginia University, P.O. Box 6315, Morgantown, West Virginia 26506-6315 (United States)

    2010-10-15T23:59:59.000Z

    Recent laser upgrades on the Hot Helicon Experiment at West Virginia University have enabled multiplexed simultaneous measurements of the ion velocity distribution function at a single location, expanding our capabilities in laser-induced fluorescence diagnostics. The laser output is split into two beams, each modulated with an optical chopper and injected perpendicular and parallel to the magnetic field. Light from the crossing point of the beams is transported to a narrow-band photomultiplier tube filtered at the fluorescence wavelength and monitored by two lock-in amplifiers, each referenced to one of the two chopper frequencies.

  17. Method for detecting binding events using micro-X-ray fluorescence spectrometry

    DOE Patents [OSTI]

    Warner, Benjamin P. (Los Alamos, NM); Havrilla, George J. (Los Alamos, NM); Mann, Grace (Hong Kong, HK)

    2010-12-28T23:59:59.000Z

    Method for detecting binding events using micro-X-ray fluorescence spectrometry. Receptors are exposed to at least one potential binder and arrayed on a substrate support. Each member of the array is exposed to X-ray radiation. The magnitude of a detectable X-ray fluorescence signal for at least one element can be used to determine whether a binding event between a binder and a receptor has occurred, and can provide information related to the extent of binding between the binder and receptor.

  18. Measurement of Pressure Dependent Fluorescence Yield of Air: Calibration Factor for UHECR Detectors

    SciTech Connect (OSTI)

    Belz, J.W.; Burt, G.W.; Cao, Z.; Chang, F.Y.; Chen, C.C.; Chen, C.W.; Chen, P.; Field, C.; Findlay, J.; Huntemeyer, Petra; Huang, M.A.; Hwang, W.-Y.P.; Iverson, R.; Jones, B.F.; Jui, C.C.H.; Kirn, M.; Lin, G.-L.; Loh, E.C.; Maestas, M.M.; Manago, N.; Martens, K.; /Montana U. /Utah U. /Taiwan, Natl. Taiwan U. /SLAC /Rutgers U., Piscataway

    2005-07-06T23:59:59.000Z

    In a test experiment at the Final Focus Test Beam of the Stanford Linear Accelerator Center, the fluorescence yield of 28.5 GeV electrons in air and nitrogen was measured. The measured photon yields between 300 and 400 nm at 1 atm and 29 C are Y(760 Torr){sup air} = 4.42 {+-} 0.73 and Y(760 Torr){sup N{sub 2}} = 29.2 {+-} 4.8 photons per electron per meter. Assuming that the fluorescence yield is proportional to the energy deposition of a charged particle traveling through air, good agreement with measurements at lower particle energies is observed.

  19. X-RAY FLUORESCENCE ANALYSIS OF HANFORD LOW ACTIVITY WASTE SIMULANTS METHOD DEVELOPMENT

    SciTech Connect (OSTI)

    Jurgensen, A; David Missimer, D; Ronny Rutherford, R

    2007-08-08T23:59:59.000Z

    The x-ray fluorescence laboratory (XRF) in the Analytical Development Directorate (ADD) of the Savannah River National Laboratory (SRNL) was requested to develop an x-ray fluorescence spectrometry method for elemental characterization of the Hanford Tank Waste Treatment and Immobilization Plant (WTP) pretreated low activity waste (LAW) stream to the LAW Vitrification Plant. The WTP is evaluating the potential for using XRF as a rapid turnaround technique to support LAW product compliance and glass former batching. The overall objective of this task was to develop an XRF analytical method that provides rapid turnaround time (<8 hours), while providing sufficient accuracy and precision to determine variations in waste.

  20. Three-dimensional x-ray fluorescence mapping of a gold nanoparticle-loaded phantom

    SciTech Connect (OSTI)

    Ren, Liqiang; Wu, Di; Li, Yuhua; Liu, Hong, E-mail: liu@ou.edu [Center for Bioengineering and School of Electrical and Computer Engineering, University of Oklahoma, Norman, Oklahoma 73019 (United States)] [Center for Bioengineering and School of Electrical and Computer Engineering, University of Oklahoma, Norman, Oklahoma 73019 (United States); Wang, Ge [Biomedical Imaging Cluster and Department of Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, New York 12180 (United States)] [Biomedical Imaging Cluster and Department of Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, New York 12180 (United States); Wu, Xizeng [Department of Radiology, University of Alabama, Birmingham, Alabama 35233 (United States)] [Department of Radiology, University of Alabama, Birmingham, Alabama 35233 (United States)

    2014-03-15T23:59:59.000Z

    Purpose : X-ray fluorescence (XRF) is a promising technique with sufficient specificity and sensitivity for identifying and quantifying features in small samples containing high atomic number (Z) materials such as iodine, gadolinium, and gold. In this study, the feasibility of applying XRF to early breast cancer diagnosis and treatment is studied using a novel approach for three-dimensional (3D) x-ray fluorescence mapping (XFM) of gold nanoparticle (GNP)-loaded objects in a physical phantom at the technical level. Methods : All the theoretical analysis and experiments are conducted under the condition of using x-ray pencil beam and a compactly integrated x-ray spectrometer. The penetrability of the fluorescence x-rays from GNPs is first investigated by adopting a combination of BR12 with 70 mm/50 mm in thickness on the excitation/emission path to mimic the possible position of tumor goldin vivo. Then, a physical phantom made of BR12 is designed to translate in 3D space with three precise linear stages and subsequently the step by step XFM scanning is performed. The experimental technique named as background subtraction is applied to isolate the gold fluorescence from each spectrum obtained by the spectrometer. Afterwards, the attenuations of both the incident primary x-ray beam with energies beyond the gold K-edge energy (80.725 keV) and the isolated gold K{sub ?} fluorescence x-rays (65.99 –69.80 keV) acquired after background subtraction are well calibrated, and finally the unattenuated K{sub ?} fluorescence counts are used to realize mapping reconstruction and to describe the linear relationship between gold fluorescence counts and corresponding concentration of gold solutions. Results : The penetration results show that the goldK{sub ?} fluorescence x-rays have sufficient penetrability for this phantom study, and the reconstructed mapping results indicate that both the spatial distribution and relative concentration of GNPs within the designed BR12 phantom can be well identified and quantified. Conclusions : Although the XFM method in this investigation is still studied at the technical level and is not yet practical for routinein vivo mapping tasks with GNPs, the current penetrability measurements and phantom study strongly suggest the feasibility to establish and develop a 3D XFM system.

  1. Fluorescence excitation enhancement by Bloch surface wave in all-polymer one-dimensional photonic structure

    SciTech Connect (OSTI)

    Fornasari, L.; Floris, F.; Patrini, M.; Guizzetti, G.; Marabelli, F., E-mail: franco.marabelli@unipv.it [Dipartimento di Fisica, Università degli Studi di Pavia, via Bassi 6, 27100 Pavia (Italy); Canazza, G.; Comoretto, D. [Dipartimento di Chimica e Chimica Industriale, Università degli Studi di Genova, via Dodecaneso 31, 16146 Genova (Italy)

    2014-08-04T23:59:59.000Z

    We demonstrate photoluminescence excitation enhancement in an all-polymer flexible one-dimensional photonic crystal structure capped with a fluorescent organic ultrathin film. When optical matching conditions between the excitation beam and the Bloch Surface Wave mode supported by the photonic structure are achieved, a ten times enhancement of the photoluminescence is observed. We notice that in these systems luminescence signal reinforcement is achieved by increasing the pump efficiency with no need of spectral resonance to the emission of the chosen fluorophore. All these features make these systems suitable candidates for easy, flexible, and cheap fluorescent sensing.

  2. COMPARATIVE COSTS OF CALIFORNIA CENTRAL STATION ELECTRICITY

    E-Print Network [OSTI]

    CALIFORNIA ENERGY COMMISSION COMPARATIVE COSTS OF CALIFORNIA CENTRAL STATION ELECTRICITY GENERATIONCann Please use the following citation for this report: Klein, Joel. 2009. Comparative Costs of California............................................................................................................................1 Changes in the Cost of Generation Model

  3. COMPARATIVE COSTS OF CALIFORNIA CENTRAL STATION ELECTRICITY

    E-Print Network [OSTI]

    Laughlin, Robert B.

    CALIFORNIA ENERGY COMMISSION COMPARATIVE COSTS OF CALIFORNIA CENTRAL STATION ELECTRICITY GENERATION and Anitha Rednam, Comparative Costs of California Central Station Electricity Generation Technologies................................................................................................... 1 CHAPTER 1: Summary of Technology Costs

  4. Comparative naval architecture analysis of diesel submarines

    E-Print Network [OSTI]

    Torkelson, Kai Oscar

    2005-01-01T23:59:59.000Z

    Many comparative naval architecture analyses of surface ships have been performed, but few published comparative analyses of submarines exist. Of the several design concept papers, reports and studies that have been written ...

  5. Educational Poverty in a Comparative Perspective

    E-Print Network [OSTI]

    Moeller, Ralf

    Educational Poverty in a Comparative Perspective: Theoretical and Empirical Implications SFB 882 Ferger Educational Poverty in a Comparative Perspective: Theoretical and Empirical Implications SFB 882 and Education: An International Comparison of Educational Poverty" This project studies the determinants

  6. INVITED: Comparative Microbial Genomics Giri Narasimhan

    E-Print Network [OSTI]

    Narasimhan, Giri

    INVITED: Comparative Microbial Genomics Giri Narasimhan Bioinformatics Research Group (Bio, comparative genomics holds the keys to decipher and mine this wealth of information. We discuss the diverse ways in which the availability of comparative genomics data allows us to answer more questions

  7. A Statistical Framework for Spatial Comparative Genomics

    E-Print Network [OSTI]

    A Statistical Framework for Spatial Comparative Genomics Rose Hoberman May 2007 CMU-CS-07, or the U.S. Government. #12;Keywords: spatial comparative genomics, comparative genomics, gene clusters, max-gap clusters, gene teams, whole genome duplication, paralogons, synteny, ortholog detection #12

  8. Modeling green fluorescent protein transcription, translation and modification as a method to obtain NF-kappaB activation profiles

    E-Print Network [OSTI]

    Laible, Allyson Marie

    2009-05-15T23:59:59.000Z

    that fluorescence increases up to 24 hours after an initial delay of approximately four hours. The fluorescence data was also used to develop a model describing significant events leading to NF-?B activation and GFP expression. In addition, a model describing...

  9. First-Principles Characterization of the Energy Landscape and Optical Spectra of Green Fluorescent Protein along the AIB

    E-Print Network [OSTI]

    Krylov, Anna I.

    First-Principles Characterization of the Energy Landscape and Optical Spectra of Green Fluorescent Supporting Information ABSTRACT: Structures and optical spectra of the green fluorescent protein (GFP) forms-form) is lowest in energy, whereas the systems with the anionic chromophore (B- and I-forms) are about 1 kcal

  10. Proton Pathways in Green Fluorescence Protein Department of Physical Chemistry, The Hebrew University of Jerusalem, Jerusalem, Israel

    E-Print Network [OSTI]

    Agmon, Noam

    Proton Pathways in Green Fluorescence Protein Noam Agmon Department of Physical Chemistry, The Hebrew University of Jerusalem, Jerusalem, Israel ABSTRACT Proton pathways in green fluorescent protein pathway exists from the active site to the protein surface, controlled by a threonine switch. A proton

  11. Single-Molecule Fluorescence Studies of Protein Folding and Conformational Xavier Michalet,* Shimon Weiss, and Marcus Jager*

    E-Print Network [OSTI]

    Michalet, Xavier

    Single-Molecule Fluorescence Studies of Protein Folding and Conformational Dynamics Xavier Michalet. Single-Molecule Fluorescence Studies of Protein Folding and Conformations at Equilibrium 1796 4-Molecule Protein Folding under Nonequilibrium Conditions 1808 6. Conclusion 1809 7. Acknowledgments 1810 8

  12. Confocal X-ray Fluorescence (XRF) Microscopy: A New Technique for the Nondestructive Compositional Depth Profiling of Paintings

    E-Print Network [OSTI]

    Gruner, Sol M.

    #12;Confocal X-ray Fluorescence (XRF) Microscopy: A New Technique for the Nondestructive tools such as x-ray fluorescence (XRF) [4,5,6,7] and proton-induced x-ray emission (PIXE) [3,8,9] to address the problem of compositional depth profiling of paintings. One XRF method consists of deducing

  13. Global analysis of Fo rster resonance energy transfer in live cells measured by fluorescence lifetime imaging microscopy exploiting

    E-Print Network [OSTI]

    van Stokkum, Ivo

    Global analysis of Fo¨ rster resonance energy transfer in live cells measured by fluorescence of Fo¨ rster resonance energy transfer (FRET) in live cells using the rise time of acceptor fluorescence those molecules that are involved in the energy-transfer process are monitored. This contrasts

  14. A Highly Selective Turn-On Colorimetric, Red Fluorescent Sensor for Detecting Mobile Zinc in Living Cells

    E-Print Network [OSTI]

    Du, Pingwu

    We describe ZRL1, a turn-on colorimetric and red fluorescent zinc ion sensor. The Zn2+-promoted ring opening of the rhodamine spirolactam ring in ZRL1 evokes a 220-fold fluorescence turn-on response. In aqueous media, ZRL1 ...

  15. Bioimaging 4 (1996) 93106. Printed in the UK Fluorescent dot counting in

    E-Print Network [OSTI]

    van Vliet, Lucas J.

    Bioimaging 4 (1996) 93­106. Printed in the UK Fluorescent dot counting in interphase cell nuclei allows the enumeration of chromosomal abnormalities in interphase cell nuclei. This process is called dot counting. To estimate the distribution of chromosomes per cell, a large number of cells have to be analysed

  16. APPLICATION OF TEMPORAL TEXTURE FEATURES TO AUTOMATED ANALYSIS OF PROTEIN SUBCELLULAR LOCATIONS IN TIME SERIES FLUORESCENCE

    E-Print Network [OSTI]

    Gordon, Geoffrey J.

    are in constant movement within the cell, we extended our studies to time series images, which contain both to identify a protein's subcellular location is to label it with fluorescent dye, take microscope images this last step. The automated approach is more objective and sensitive than visual examination, and single

  17. Compact fluorescent lamp using horizontal and vertical insulating septums and convective venting geometry

    DOE Patents [OSTI]

    Siminovitch, M.

    1998-02-10T23:59:59.000Z

    A novel design is described for a compact fluorescent lamp, including a lamp geometry which will increase light output and efficacy of the lamp in a base down operating position by providing horizontal and vertical insulating septums positioned in the ballast compartment of the lamp to provide a cooler coldspot. Selective convective venting provides additional cooling of the ballast compartment. 9 figs.

  18. Red Fluorescent Protein pH Biosensor to Detect Concentrative Nucleoside Transport*S

    E-Print Network [OSTI]

    Campbell, Robert E.

    Red Fluorescent Protein pH Biosensor to Detect Concentrative Nucleoside Transport*S Received concentrative nucleoside transporter, hCNT3, medi- ates Na /nucleoside and H /nucleoside co-transport. We describe a new approach to monitor H /uridine co-transport in cultured mammalian cells, using a p

  19. Understanding Blue-to-Red Conversion in Monomeric Fluorescent Timers and Hydrolytic Degradation of Their

    E-Print Network [OSTI]

    Verkhusha, Vladislav V.

    Understanding Blue-to-Red Conversion in Monomeric Fluorescent Timers and Hydrolytic Degradation-FT (chromophore Met66-Tyr67-Gly68) and its precursor with blocked blue-to- red conversion Blue102 (chromophore Leu data suggest that blue-to-red conversion, taking place in Fast-FT and in related FTs, is associated

  20. FLUORESCENCE AND FIBER-OPTICS BASED REAL-TIME THICKNESS SENSOR FOR DYNAMIC LIQUID FILMS

    E-Print Network [OSTI]

    Narain, Amitabh

    /analyzed the incident reflected waves to identify and measure the total transit time of the sound wave (of known wave-speed1 FLUORESCENCE AND FIBER-OPTICS BASED REAL-TIME THICKNESS SENSOR FOR DYNAMIC LIQUID FILMS T. W. Ng/disadvantages of many known liquid film thickness sensing devices (viz. conductivity probes, reflectance based fiber

  1. Laser-excited atomic fluorescence spectrometry in a graphite furnace with an

    E-Print Network [OSTI]

    Michel, Robert G.

    must provide high peak energy above sequentially with the analysis time determined primarilyLaser-excited atomic fluorescence spectrometry in a graphite furnace with an optical parametric for electrothermal atomic excited atomic ¯ uorescence spectrometry (LEAFS ) in a absorption spectrometry (ETAAS

  2. Accretion, fluorescent X-ray emission and flaring magnetic structures in YSOs

    E-Print Network [OSTI]

    F. Favata

    2004-12-20T23:59:59.000Z

    I present some recent developments on high-energy phenomena in YSOs, concentrating on the new evidence for accretion-induced X-ray emission in YSOs, for Fe 6.4 keV fluorescent emission from the disks of YSOs and for very long magnetic structures responsible for intense X-ray flares, likely connecting the star and the circumstellar disk.

  3. Improved fluorescence-enhanced optical imaging and tomography by enhanced excitation light rejection

    E-Print Network [OSTI]

    Hwang, Kil Dong

    2009-05-15T23:59:59.000Z

    match for tomographic reconstruction of one (1 cm3) and two (0.1 cm3) targets in a 1087 cm3 of breast phantom. Ultimately, this work improves the sensitivity of NIR fluorescence imaging by enhancing the rejection of excitation light and shows...

  4. Iron Fluorescent Line Emission from Young Stellar Objects in the Orion Nebula

    E-Print Network [OSTI]

    M. Tsujimoto; E. D. Feigelson; N. Grosso; G. Micela; Y. Tsuboi; F. Favata; H. Shang; J. H. Kastner

    2004-12-25T23:59:59.000Z

    We present the result of a systematic search for the iron Kalpha fluorescent line at 6.4 keV among 1616 X-ray sources detected by ultra-deep Chandra observations of the Orion Nebula Cluster and the obscured Orion Molecular Cloud 1 population as part of the Chandra Orion Ultra-deep Project (COUP). Seven sources are identified to have an excess emission at 6.4 keV among 127 control sample sources with significant counts in the 6.0-9.0 keV band. These seven sources are young stellar objects (YSOs) characterized by intense flare-like flux variations, thermal spectra, and near-infrared (NIR) counterparts. The observed equivalent widths of the line cannot be attributed to the fluorescence by interstellar or circumstellar matter along the line of sight. The X-ray spectral fits and NIR colors of the 6.4 keV sources show that these sources have X-ray absorption of > 1x10^22 cm^(-2) and NIR excess emission, which is not expected when the fluorescence occurs at the stellar photosphere. We therefore conclude that the iron fluorescent line of YSOs arises from reflection off of circumstellar disks, which are irradiated by the hard X-ray continuum emission of magnetic reconnection flares.

  5. Fluorescent probes for non-invasive bioenergetic studies of whole cyanobacterial cells

    E-Print Network [OSTI]

    Roegner, Matthias

    Fluorescent probes for non-invasive bioenergetic studies of whole cyanobacterial cells Markus of bioenergetic processes in whole cells of the cyanobacterium Synechocystis sp. PCC 6803. Acridine yellow to be created. In sum- mary, bioenergetic £uorescence measurement com- bines the advantages of an easy

  6. Fluorescence technique for on-line monitoring of state of hydrogen-producing microorganisms

    DOE Patents [OSTI]

    Seibert, Michael (Lakewood, CO); Makarova, Valeriya (Golden, CO); Tsygankov, Anatoly A. (Pushchino, RU); Rubin, Andrew B. (Moscow, RU)

    2007-06-12T23:59:59.000Z

    In situ fluorescence method to monitor state of sulfur-deprived algal culture's ability to produce H.sub.2 under sulfur depletion, comprising: a) providing sulfur-deprived algal culture; b) illuminating culture; c) measuring onset of H.sub.2 percentage in produced gas phase at multiple times to ascertain point immediately after anerobiosis to obtain H.sub.2 data as function of time; and d) determining any abrupt change in three in situ fluorescence parameters; i) increase in F.sub.t (steady-state level of chlorophyll fluorescence in light adapted cells); ii) decrease in F.sub.m', (maximal saturating light induced fluorescence level in light adapted cells); and iii) decrease in .DELTA.F/F.sub.m'=(F.sub.m'-F.sub.t)/F.sub.m' (calculated photochemical activity of photosystem II (PSII) signaling full reduction of plastoquinone pool between PSII and PSI, which indicates start of anaerobic conditions that induces synthesis of hydrogenase enzyme for subsequent H.sub.2 production that signal oxidation of plastoquinone pool asmain factor to regulate H.sub.2 under sulfur depletion.

  7. Subsurface contaminant monitoring by laser fluorescence excitation-emission spectroscopy in a cone penetrometer probe

    SciTech Connect (OSTI)

    Lin, J.; Hart, S.J.; Wang, W.; Namytchkine, D.; Kenny, J.E. [Tufts Univ., Medford, MA (United States). Dept. of Chemistry

    1995-12-31T23:59:59.000Z

    A laser-induced fluorescence (LIF) excitation-emission matrix (EEM) probe has been developed for subsurface monitoring of fluorescent organic contaminants. The fourth harmonic of a flashlamp-pumped Nd:YAG laser (at 266 nm) is used to pump a Raman shifter. Up to ten laser beams (in the wavelength region of 258 to 379 nm) from the Raman shifter are launched into optical fibers that conduct the light to the probe near the tip of the cone penetrometer. The fluorescence emission is excited through ten separate sapphire windows and collected by ten collection fibers that conduct the fluorescence to a spectrograph/CCD detection system. This probe allows real-time collection of LIF-EEMs of contaminants adsorbed on solids or dissolved in groundwater. LIF-EEMs provide a substantial amount of spectral information that can be used to determine the composition and quantity of contaminants in soils. The system was tested and calibrated in the laboratory. Spectra of different organic contaminants were measured in aqueous solutions, in organic solvents, and in different types of soils.

  8. Determination of hydrocarbons in sea water via UV-induced total fluorescence spectroscopy

    SciTech Connect (OSTI)

    Muroski, A.R.; Booksh, K.S.; Myrick, M.L. [Univ. of South Carolina, Columbia, SC (United States)

    1995-12-31T23:59:59.000Z

    The increasingly ubiquitous presence of anthropogenic hydrocarbons in oceans, lakes, and rivers necessitates the development of practical means of determining such pollutants in natural waters. Monitoring the subsurface progression and eventual fate of hydrocarbons from a particular source in the marine environment requires a technique amenable to rapid data acquisition and evaluation. Total UV fluorescence spectroscopy meets this requirement, while providing sufficient information in the excitation/emission matrix (EEM) for resolution of individual compounds in a complex aqueous mixture. The large amount of information available in an EEM can be made practical, however, only through the use of third-order statistical spectral analysis. Simultaneous excitation and collection of fluorescence at multiple wavelengths was accomplished using an imaging spectrofluorometer. Application of principal component analysis (PCA) to the resulting EEMs reveals the number of spectrally-distinct substances in oil/water mixtures. The contribution of each of these fluorophores to the matrix can then be determined by means of parallel factor analysis (PARAFAC), providing a solution which is unique for a particular component. Individual hydrocarbons, such as naphthalene - a major UV-fluorescent component of gasoline, have been determined at low ppb levels in sea water characterized by a fluorescence background produced by petroleum and humic acids.

  9. Metal-atom fluorescence from the quenching of metastable rare gases by metal carbonyls

    SciTech Connect (OSTI)

    Hollingsworth, W.E.

    1982-11-01T23:59:59.000Z

    A flowing afterglow apparatus was used to study the metal fluorescence resulting from the quenching of metastable rare-gas states by metal carbonyls. The data from the quenching or argon, neon, and helium by iron and nickel carbonyl agreed well with a restricted degree of freedom model indicating a concerted bond-breaking dissociation.

  10. Estimation of membrane lateral pressure in living cells by means of multidimensional confocal fluorescence microscopy

    E-Print Network [OSTI]

    Rosso, Lula

    -labeled phospholipids. We report an alternative strategy based on the intramolecular fluorescence energy transfer Kensington Campus, London SW7 2AZ, UK Considerable pressures (many tens of atmospheres) are generated within between the energy necessary to keep the hydrocarbon chains away from water and the energy generated

  11. A Microfluidic Device for Temporally Controlled Gene Expression and Long-Term Fluorescent Imaging in

    E-Print Network [OSTI]

    Siggia, Eric

    A Microfluidic Device for Temporally Controlled Gene Expression and Long-Term Fluorescent Imaging of the cell cycle. Over the past ten years, microfluidic techniques in cell biology have emerged that allow a microfluidic flow cell to grow Saccharomyces Cerevisiae for more than 8 generations (

  12. FOCUS www.rsc.org/loc | Lab on a Chip Improving fluorescence detection in lab on

    E-Print Network [OSTI]

    Fygenson, Deborah Kuchnir

    ) graft on glass. Two cell populations are separately labeled with live cell dyes and are mixed and seeded and sensitivity, due primarily to sub-optimal light collection. More sensi- tive, higher resolution LOC devices for performing high-sensitivity and high-resolution fluo- rescence detection within LOC devices. Fluorescence

  13. Single-molecule protein folding: Diffusion fluorescence resonance energy transfer studies

    E-Print Network [OSTI]

    Croquette, Vincent

    Single-molecule protein folding: Diffusion fluorescence resonance energy transfer studies for protein folding studies and has been extensively stud- ied, both experimentally (at the ensemble level concentration. It is shown that new infor- mation about different aspects of the protein folding reaction can

  14. Video-Rate Scanning Two-Photon Excitation Fluorescence Microscopy and Ratio Imaging with Cameleons

    E-Print Network [OSTI]

    Tsien, Roger Y.

    Video-Rate Scanning Two-Photon Excitation Fluorescence Microscopy and Ratio Imaging with Cameleons ABSTRACT A video-rate (30 frames/s) scanning two-photon excitation microscope has been successfully tested 690 to 1050 nm, prechirper optics for laser pulse-width compression, resonant galvanometer for video

  15. Large single-molecule fluorescence enhancements produced by a bowtie nanoantenna

    E-Print Network [OSTI]

    Fan, Shanhui

    Large single-molecule fluorescence enhancements produced by a bowtie nanoantenna Anika Kinkhabwala1, the highly enhanced optical fields of lithographically fabricated bowtie nanoantennas9 provide a structure that is more controllable and amenable to integration. Using gold bowties, we observe enhancements of a single

  16. Filter-fluorescer measurement of low-voltage simulator x-ray energy spectra

    SciTech Connect (OSTI)

    Baldwin, G.T.; Craven, R.E.

    1986-01-01T23:59:59.000Z

    X-ray energy spectra of the Maxwell Laboratories MBS and Physics International Pulserad 737 were measured using an eight-channel filter-fluorescer array. The PHOSCAT computer code was used to calculate channel response functions, and the UFO code to unfold spectrum.

  17. absorption laser-induced fluorescence: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    absorption laser-induced fluorescence First Page Previous Page 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 Next Page Last Page Topic Index 1 Absolute...

  18. Petrographic characterization of Kentucky coals: relationship between sporinite spectral fluorescence and coal rank of selected western Kentucky coals. Final report, Part I. [Vitrinite

    SciTech Connect (OSTI)

    Poe, S.H.; Hower, J.C.

    1983-01-01T23:59:59.000Z

    A total of 43 coal samples were analyzed - the majority from western Kentucky, with a few from Pennsylvania for comparative purposes - using quantitative fluorescence microscopy of sporinite to determine if coal rank as determined by vitrinite maximum reflectance could be predicted by data gathered from selected fluorescence parameters. All eight parameters (wavelength of highest intensity, area under curve to the left of the peak, area in the blue wavelengths (400 to 500 nm), green (500 to 570 nm), yellow (570 to 630 nm), blue-red ratio, and red-green ratio were found to statistically predict coal rank. The general research hypothesis, which included all the variables, had a R/sup 2/ = 0.354. The results of the step-wise regression yielded red and yellow (collective R/sup 2/ = 0.341) as the best predictor variables of coal rank. The individual parameters of area of red wavelength and blue-red ratio accounted for the greatest variance in predicting coal rank, while the parameter yellow area was the least predictive of coal rank. 31 references, 7 figures, 5 tables.

  19. ISSUANCE 2015-06-17: Energy Conservation Standards for Fluorescent Lamp Ballasts, Notice of Public Meeting and Availability of the Framework Document

    Broader source: Energy.gov [DOE]

    Energy Conservation Standards for Fluorescent Lamp Ballasts, Notice of Public Meeting and Availability of the Framework Document

  20. The use of fluorescent intrabodies to detect endogenous gankyrin in living cancer cells

    SciTech Connect (OSTI)

    Rinaldi, Anne-Sophie; Freund, Guillaume; Desplancq, Dominique; Sibler, Annie-Paule; Baltzinger, Mireille [Ecole Supérieure de Biotechnologie de Strasbourg, UMR 7242, CNRS/Université de Strasbourg, boulevard Sébastien Brant, 67412 Illkirch (France); Rochel, Natacha [Institut de Génétique et de Biologie Moléculaire et Cellulaire, UMR 7104, CNRS/INSERM/Université de Strasbourg, rue Laurent Fries, 67404 Illkirch (France); Mély, Yves; Didier, Pascal [Faculté de Pharmacie, UMR 7213, CNRS/Université de Strasbourg, route du Rhin, 67401 Illkirch (France); Weiss, Etienne, E-mail: eweiss@unistra.fr [Ecole Supérieure de Biotechnologie de Strasbourg, UMR 7242, CNRS/Université de Strasbourg, boulevard Sébastien Brant, 67412 Illkirch (France)

    2013-04-01T23:59:59.000Z

    Expression of antibody fragments in mammalian cells (intrabodies) is used to probe the target protein or interfere with its biological function. We previously described the in vitro characterisation of a single-chain Fv (scFv) antibody fragment (F5) isolated from an intrabody library that binds to the oncoprotein gankyrin (GK) in solution. Here, we have isolated several other scFvs that interact with GK in the presence of F5 and tested whether they allow, when fused to fluorescent proteins, to detect by FRET endogenous GK in living cells. The binding of pairs of scFvs to GK was analysed by gel filtration and the ability of each scFv to mediate nuclear import/export of GK was determined. Binding between scFv-EGFP and RFP-labelled GK in living cells was detected by fluorescence lifetime imaging microscopy (FLIM). After co-transfection of two scFvs fused to EGFP and RFP, respectively, which form a tri-molecular complex with GK in vitro, FRET signal was measured. This system allowed us to observe that GK is monomeric and distributed throughout the cytoplasm and nucleus of several cancer cell lines. Our results show that pairs of fluorescently labelled intrabodies can be monitored by FLIM–FRET microscopy and that this technique allows the detection of lowly expressed endogenous proteins in single living cells. Highlights: ? Endogenous GK in living cells was targeted with pairs of fluorescently-tagged scFvs. ? Tri-molecular complexes containing two scFvs and one molecule GK were formed. ? GK was detected using fluorescence lifetime-based FRET imaging. ? GK is monomeric and homogeneously distributed in several cancer cell lines. ? This technique may have many applications in live-cell imaging of endogenous proteins.

  1. Recovery of yttrium from cathode ray tubes and lamps’ fluorescent powders: experimental results and economic simulation

    SciTech Connect (OSTI)

    Innocenzi, V., E-mail: valentina.innocenzi1@univaq.it; De Michelis, I.; Ferella, F.; Vegliò, F.

    2013-11-15T23:59:59.000Z

    Highlights: • Fluorescent powder of lamps. • Fluorescent powder of cathode ray rubes. • Recovery of yttrium from fluorescent powders. • Economic simulation for the processes to recover yttrium from WEEE. - Abstract: In this paper, yttrium recovery from fluorescent powder of lamps and cathode ray tubes (CRTs) is described. The process for treating these materials includes the following: (a) acid leaching, (b) purification of the leach liquors using sodium hydroxide and sodium sulfide, (c) precipitation of yttrium using oxalic acid, and (d) calcinations of oxalates for production of yttrium oxides. Experimental results have shown that process conditions necessary to purify the solutions and recover yttrium strongly depend on composition of the leach liquor, in other words, whether the powder comes from treatment of CRTs or lamp. In the optimal experimental conditions, the recoveries of yttrium oxide are about 95%, 55%, and 65% for CRT, lamps, and CRT/lamp mixture (called MIX) powders, respectively. The lower yields obtained during treatments of MIX and lamp powders are probably due to the co-precipitation of yttrium together with other metals contained in the lamps powder only. Yttrium loss can be reduced to minimum changing the experimental conditions with respect to the case of the CRT process. In any case, the purity of final products from CRT, lamps, and MIX is greater than 95%. Moreover, the possibility to treat simultaneously both CRT and lamp powders is very important and interesting from an industrial point of view since it could be possible to run a single plant treating fluorescent powder coming from two different electronic wastes.

  2. Energy Savings and Green Initiatives Project Grant

    SciTech Connect (OSTI)

    Kathy MacLennan

    2011-11-21T23:59:59.000Z

    This project entails retrofitting all four foot, 2, 3 and 4 bulb 40 watt T12 fixtures to T8 28 watt and 150 watt incandescent to 26 watt compact fluorescent bulbs. In total, 2,086 fixtures will be retrofitted

  3. UMass Amherst Green Office Program Checklist v2.0

    E-Print Network [OSTI]

    Massachusetts at Amherst, University of

    lamps we use CFLs (Compact Fluorescent Light bulbs) or LED bulbs E7. We use power strips as central AND RECYCLING Prerequisites WRP1. We have a designated area in our supply closet, or elsewhere in our office clips, etc). WRP2. All common areas and work areas (cube or office) has a recycling bin Credits WR1

  4. Fluorescence Efficiency and Stability of Radio-Pure Tetraphenyl-butadiene Based Coatings for VUV Light Detection in Cryogenic Environments

    E-Print Network [OSTI]

    Baudis, Laura; Dressler, Rugard; Piastra, Francesco; Usoltsev, Ilya; Walter, Manuel

    2015-01-01T23:59:59.000Z

    The detection of VUV scintillation light, e.g. in (liquid) argon detectors, commonly includes a reflector with a fluorescent coating, converting UV photons to visible light. The light yield of these detectors depends directly on the conversion efficiency. Several coating/reflector combinations were produced using VM2000, a specular reflecting multi layer polymer, and Tetratex, a diffuse reflecting PTFE fabric, as reflector foils. The efficiency of these coatings was optimised and has been measured in a dedicated liquid argon setup built at the University of Zurich. It employs a small, 1.3 kg LAr cell viewed by a 3-inch, low radioactivity PMT of type R11065-10 from Hamamatsu. The cryogenic stability of these coatings was additionally studied. The optimum reflector/coating combination was found to be Tetratex dip coated with Tetraphenyl-butadiene with a thickness of 0.9 mg/cm$^2$ resulting in a 3.6 times higher light yield compared to uncoated VM2000. Its performance was stable in long term measurements, ran up...

  5. Dark-state suppression and optimization of laser cooling and fluorescence in a trapped alkaline-earth-metal single ion

    E-Print Network [OSTI]

    T. Lindvall; M. Merimaa; I. Tittonen; A. A. Madej

    2012-12-19T23:59:59.000Z

    We study the formation and destabilization of dark states in a single trapped 88Sr+ ion caused by the cooling and repumping laser fields required for Doppler cooling and fluorescence detection of the ion. By numerically solving the time-dependent density matrix equations for the eight-level system consisting of the sublevels of the 5s 2S1/2, 5p 2P1/2, and 4d 2D3/2 states, we analyze the different types of dark states and how to prevent them in order to maximize the scattering rate, which is crucial for both the cooling and the detection of the ion. The influence of the laser linewidths and ion motion on the scattering rate and the dark resonances is studied. The calculations are then compared with experimental results obtained with an endcap ion trap system located at the National Research Council of Canada and found to be in good agreement. The results are applicable also to other alkaline earth ions and isotopes without hyperfine structure.

  6. Comparative phosphoproteomics reveals components of host cell...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    only hyper-cytotoxic but also were phagocytosed at much higher rates compared to the wild type parent strain. To elucidate the cellular signaling underlying this enhanced...

  7. Treatment of exhaust fluorescent lamps to recover yttrium: Experimental and process analyses

    SciTech Connect (OSTI)

    De Michelis, Ida, E-mail: ida.demichelis@univaq.it [University of L'Aquila, Department of Chemistry, Chemical Engineering and Materials - Ex-Optimes Loc., Campo di Pile, 67100 L'Aquila (Italy); Ferella, Francesco, E-mail: francesco.ferella@univaq.it [University of L'Aquila, Department of Chemistry, Chemical Engineering and Materials - Ex-Optimes Loc., Campo di Pile, 67100 L'Aquila (Italy); Varelli, Ennio Fioravante [University of L'Aquila, Department of Chemistry, Chemical Engineering and Materials - Ex-Optimes Loc., Campo di Pile, 67100 L'Aquila (Italy); Veglio, Francesco, E-mail: francesco.veglio@univaq.it [University of L'Aquila, Department of Chemistry, Chemical Engineering and Materials - Ex-Optimes Loc., Campo di Pile, 67100 L'Aquila (Italy)

    2011-12-15T23:59:59.000Z

    Highlights: > Recovery of yttrium from spent fluorescent lamps by sulphuric acid leaching. > The use of sulphuric acid allows to reduce calcium dissolutions. > Main contaminant of fluorescent powder are Si, Pb, Ca and Ba. > Hydrated yttrium oxalate, recovered by selective precipitation, is quite pure (>90%). > We have studied the whole process for the treatment of dangerous waste (plant capability). - Abstract: The paper deals with recovery of yttrium from fluorescent powder coming from dismantling of spent fluorescent tubes. Metals are leached by using different acids (nitric, hydrochloric and sulphuric) and ammonia in different leaching tests. These tests show that ammonia is not suitable to recover yttrium, whereas HNO{sub 3} produces toxic vapours. A full factorial design is carried out with HCl and H{sub 2}SO{sub 4} to evaluate the influence of operating factors. HCl and H{sub 2}SO{sub 4} leaching systems give similar results in terms of yttrium extraction yield, but the last one allows to reduce calcium extraction with subsequent advantage during recovery of yttrium compounds in the downstream. The greatest extraction of yttrium is obtained by 20% w/v S/L ratio, 4 N H{sub 2}SO{sub 4} concentration and 90 deg. C. Yttrium and calcium yields are nearly 85% and 5%, respectively. The analysis of variance shows that acid concentration alone and interaction between acid and pulp density have a significant positive effect on yttrium solubilization for both HCl and H{sub 2}SO{sub 4} medium. Two models are empirically developed to estimate yttrium and calcium concentration during leaching. Precipitation tests demonstrate that at least the stoichiometric amount of oxalic acid is necessary to recover yttrium efficiently and a pure yttrium oxalate n-hydrate can be produced (99% grade). The process is economically feasible if other components of the fluorescent lamps (glass, ferrous and non-ferrous scraps) are recovered after the equipment dismantling and valorized, besides the cost that is usually paid to recycling companies for collection, treatment or final disposal of such fluorescent powders.

  8. Treatability study for removal of leachable mercury in crushed fluorescent lamps

    SciTech Connect (OSTI)

    Bostick, W.D.; Beck, D.E.; Bowser, K.T. [and others

    1996-02-01T23:59:59.000Z

    Nonserviceable fluorescent lamps removed from radiological control areas at the Oak Ridge Department of Energy facilities have been crushed and are currently managed as mixed waste (hazardous and radiologically contaminated). We present proposed treatment flowsheets and supporting treatability study data for conditioning this solid waste residue so that it can qualify for disposal in a sanitary landfill. Mercury in spent fluorescent lamps occurs primarily as condensate on high-surface-area phosphor material. It can be solubilized with excess oxidants (e.g., hypochlorite solution) and stabilized by complexation with halide ions. Soluble mercury in dechlorinated saline solution is effectively removed by cementation with zero-valent iron in the form of steel wool. In packed column dynamic flow testing, soluble mercury was reduced to mercury metal and insoluble calomel, loading > 1.2 g of mercury per grain of steel wool before an appreciable breakthrough of soluble mercury in the effluent.

  9. Enhanced-locality fiber-optic two-photon-fluorescence live-brain interrogation

    SciTech Connect (OSTI)

    Fedotov, I. V.; Doronina-Amitonova, L. V. [International Laser Center, Physics Department, M.V. Lomonosov Moscow State University, Moscow 119992 (Russian Federation); Russian Quantum Center, ul. Novaya 100, Skolkovo, Moscow Region 1430125 (Russian Federation); Kurchatov Institute National Research Center, Moscow (Russian Federation); Sidorov-Biryukov, D. A.; Fedotov, A. B. [International Laser Center, Physics Department, M.V. Lomonosov Moscow State University, Moscow 119992 (Russian Federation); Russian Quantum Center, ul. Novaya 100, Skolkovo, Moscow Region 1430125 (Russian Federation); Anokhin, K. V. [Kurchatov Institute National Research Center, Moscow (Russian Federation); P.K. Anokhin Institute of Normal Physiology, Russian Academy of Medical Sciences, Moscow (Russian Federation); Kilin, S. Ya. [B.I. Stepanov Institute of Physics, National Academy of Sciences of Belarus, Minsk (Belarus); Sakoda, K. [National Institute for Materials Science, 1-1 Namiki, Tsukuba 305-0044 (Japan); Zheltikov, A. M. [International Laser Center, Physics Department, M.V. Lomonosov Moscow State University, Moscow 119992 (Russian Federation); Russian Quantum Center, ul. Novaya 100, Skolkovo, Moscow Region 1430125 (Russian Federation); Department of Physics and Astronomy, Texas A and M University, College Station, Texas 77843 (United States); Center of Photochemistry, Russian Academy of Sciences, ul. Novatorov 7a, Moscow 117421 (Russian Federation)

    2014-02-24T23:59:59.000Z

    Two-photon excitation is shown to substantially enhance the locality of fiber-based optical interrogation of strongly scattering biotissues. In our experiments, a high-numerical-aperture, large-core-are fiber probe is used to deliver the 200-fs output of a 100-MHz mode-locked ytterbium fiber laser to samples of live mouse brain, induce two-photon fluorescence of nitrogen–vacancy centers in diamond markers in brain sample. Fiber probes with a high numerical aperture and a large core area are shown to enable locality enhancement in fiber-laser–fiber-probe two-photon brain excitation and interrogation without sacrificing the efficiency of fluorescence response collection.

  10. NIST energy related inventions: Electronic starter device for fluorescent lamps. Interim report, August--October, 1997

    SciTech Connect (OSTI)

    Johnson, S.A.

    1997-12-01T23:59:59.000Z

    From the Scope of Work document which accompanied the original proposal, three silicon devices were anticipated for development, simulation, and quality assurance fabrication. The status of these are in the same format as the Scope of Work...Attachment-A-: Task 1--design and simulation; Task 2--prototype tooling; Task 3--test engineering; Task 4--product tooling; Task 5--package tooling/manufacturing design and assembly. It is felt the program will meet it`s stated goals of producing a low cost, high performance fluorescent lamp starter which will lower the acquisition and operating cost of fluorescent technology...thus saving significant amounts of energy. The likelihood of success is even greater, now that the TN22 component has been qualified. The challenges of creating a custom ASIC, while still significant, are within the skill and expertise level or the assigned engineers.

  11. Laser induced fluorescence imaging of thermal damage in polymer matrix composites

    SciTech Connect (OSTI)

    Wachter, E.A.; Fisher, W.G.; Meyer, K.E.

    1996-12-31T23:59:59.000Z

    A simple, fluorescence-based imaging system was developed for identifying regions of thermal damage in polymer-matrix composites (PMCs). PMCs have important applications where low weight and high mechanical strength are needed. One concern in the aerospace industry is the tendency of some PMC materials to become irreversibly damaged when exposed to high temperatures. Traditional nondestructive evaluation (NDE) techniques are capable of detecting physical flaws, such as cracks and delaminations, but have not proven effective for detecting initial heat damage, which occurs on a molecular scale. Spectroscopic techniques such as laser-induced fluorescence provide an attractive means for detecting thermal damage on large, irregularly shaped surfaces. This paper describes instrumentation capable of rapidly detecting thermal damage in graphite/epoxy components.

  12. Dynamically Controlled Resonance Fluorescence from a Doubly Dressed Solid-State Single Emitter

    E-Print Network [OSTI]

    Yu He; Y. -M. He; J. Liu; Y. -J. Wei; H. Ramirez; M. Atatüre; C. Schneider; M. Kamp; S. Höfling; C. -Y. Lu; J. -W. Pan

    2014-11-22T23:59:59.000Z

    We report the first experimental demonstration of interference-induced spectral line elimination predicted by Zhu and Scully [Phys. Rev. Lett. 76, 388 (1996)] and Ficek and Rudolph [Phys. Rev. A 60, 4245 (1999)]. We drive an exciton transition of a self-assembled quantum dot in order to realize a two-level system exposed to bichromatic laser field and observe nearly complete elimination of the resonance fluorescence spectral line at the driving laser frequency. This is caused by quantum interference between coupled transitions among the doubly dressed excitonic states, without population trapping. We also demonstrate multiphoton ac Stark effect with shifted subharmonic resonances and dynamical modifications of resonance fluorescence spectra by using double dressing.

  13. Femtosecond lasing from a fluorescent protein in a one dimensional random cavity

    E-Print Network [OSTI]

    Drane, T M; Shapiro, M; Milner, V

    2015-01-01T23:59:59.000Z

    We present evidence of ultrafast random lasing from the fluorescent protein DsRed2 embedded in a random one-dimensional cavity. Lasing is achieved when a purified protein solution, placed inside a layered random medium, is optically excited with a femtosecond pump pulse in the direction perpendicular to the plane of random layers. We demonstrate that pumping with ultrashort pulses resulted in a lasing threshold two orders of magnitude lower than that found for nanosecond excitation.

  14. A Fluorescent Aerogel for Capture and Identification of Interplanetary and Interstellar Dust

    E-Print Network [OSTI]

    Gerardo Dominguez; Andrew J. Westphal; Mark L. F. Phillips; Steven M. Jones

    2003-03-27T23:59:59.000Z

    Contemporary interstellar dust has never been analyzed in the laboratory, despite its obvious astronomical importance and its potential as a probe of stellar nucleosynthesis and galactic chemical evolution. Here we report the discovery of a novel fluorescent aerogel which is capable of capturing hypervelocity dust grains and passively recording their kinetic energies. An array of these "calorimetric" aerogel collectors in low earth orbit would lead to the capture and identification of large numbers of interstellar dust grains.

  15. 2015-01-28 Issuance: Test Procedure for Fluorescent Lamp Ballasts; Final Rule Correction

    Broader source: Energy.gov [DOE]

    This document is a pre-publication Federal Register final rule correction regarding test procedures for fluorescent lamp ballasts, as issued by the Deputy Assistant Secretary for Energy Efficiency on January 28, 2014. Though it is not intended or expected, should any discrepancy occur between the document posted here and the document published in the Federal Register, the Federal Register publication controls. This document is being made available through the Internet solely as a means to facilitate the public's access to this document.

  16. Evaluation of Portable X-Ray Fluorescence (XRF) Analyzer for Zirconium-Thickness Measurements

    SciTech Connect (OSTI)

    Glenn Moore

    2013-09-01T23:59:59.000Z

    This Technical Evaluation Report provides details of preliminary testing/experiments performed using a handheld X-ray fluorescence analyzer. The analyzer will be utilized in upcoming fuel-foil-rolling optimization studies at the INL. The studies are being performed in support of DOE’s Office of Global Threat Reduction -- Reactor Conversion Subprogram. Details of the equipment used, operating parameters, and measurement results are provided in this report.

  17. Precise Measurement of the Absolute Yield of Fluorescence Photons in Atmospheric Gases

    SciTech Connect (OSTI)

    Ave, M.; /Karlsruhe, Inst. Technol.; Bohacova, M.; /Chicago U., EFI; Daumiller, K.; /Karlsruhe, Inst. Technol.; Di Carlo, P.; /INFN, Aquila; Di Giulio, C.; /INFN, Rome; Luis, P.Facal San; /Chicago U., EFI; Gonzales, D.; /Karlsruhe U., EKP; Hojvat, C.; /Fermilab; Horandel, J.R.; /Nijmegen U., IMAPP; Hrabovsky, M.; /Palacky U.; Iarlori, M.; /INFN, Aquila /Karlsruhe, Inst. Technol.

    2011-01-01T23:59:59.000Z

    We have performed a measurement of the absolute yield of fluorescence photons at the Fermilab Test Beam. A systematic uncertainty at 5% level was achieved by the use of Cherenkov radiation as a reference calibration light source. A cross-check was performed by an independent calibration using a laser light source. A significant improvement on the energy scale uncertainty of Ultra-High Energy Cosmic Rays is expected.

  18. Development of a radiative transport based, fluorescence-enhanced, frequency-domain small animal imaging system

    E-Print Network [OSTI]

    Rasmussen, John C.

    2009-05-15T23:59:59.000Z

    Dissertation by JOHN C. RASMUSSEN Submitted to the Office of Graduate Studies of Texas A&M University in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY Approved by: Chair of Committee, Eva M. Sevick...-Enhanced, Frequency-Domain Small Animal Imaging System. (December 2006) John C. Rasmussen, B.S., University of Oklahoma Chair of Advisory Committee: Dr. Eva M. Sevick-Muraca Herein we present the development of a fluorescence-enhanced, frequency- domain radiative...

  19. Simulation of air shower image in fluorescence light based on energy deposits derived from CORSIKA

    E-Print Network [OSTI]

    D. Gora; D. Heck; P. Homola; H. Klages; J. Pekala; M. Risse; B. Wilczynska; H. Wilczynski

    2004-03-01T23:59:59.000Z

    Spatial distributions of energy deposited by an extensive air shower in the atmosphere through ionization, as obtained from the CORSIKA simulation program, are used to find the fluorescence light distribution in the optical image of the shower. The shower image derived in this way is somewhat smaller than that obtained from the NKG lateral distribution of particles in the shower. The size of the image shows a small dependence on the primary particle type.

  20. A spreadsheet for analyzing the in situ performance of fluorescent luminaires

    SciTech Connect (OSTI)

    Rubinstein, F.; Zhang, Chin.

    1991-08-01T23:59:59.000Z

    A spreadsheet program for determining system efficacy, power input and light output of common 4 ft fluorescent lighting systems under realistic operating conditions is described. The program uses accepted IES engineering principles to precisely account for ballast factor, existing thermal conditions and maintenance practices. The spreadsheet, which includes a data base of lamp and ballast performance data, can be used to calculate the cost-effectiveness of many common lighting retrofits.