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Sample records for find unedited transcripts

  1. QTR - unedited comments | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    - unedited comments QTR - unedited comments Comments received in response to the DOE-QTR Request For Information (24.47 MB) More Documents & Publications Table of QTR comments in response to Federal Register RFI Table of QTR comments in response to Federal Register RFI QTR table of respondents

  2. Finding

    Office of Legacy Management (LM)

    Finding of No Significant Impact Ground Water Compliance at the Grand Junction UMTRA Project Site (Climax Uranium Millsite) September 1999 U.S. Department of Energy Grand Junction ...

  3. Spring 2006 ASA Meeting Disclaimer

    U.S. Energy Information Administration (EIA) Indexed Site

    6 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration In two adjacent files you may find unedited transcripts of EIA's spring 2006 meeting with the American Statistical Association Committee on Energy Statistics. Beginning with the fall 2003 meeting, EIA no longer edits these transcripts. Summaries of previous meetings may be found to the right of the Thursday and Friday transcripts. The public meeting took place April 6 and 7,

  4. Spring 2006 ASA Meeting Disclaimer

    U.S. Energy Information Administration (EIA) Indexed Site

    7 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration In two adjacent files you will find unedited transcripts of EIA's spring 2007 meeting with the American Statistical Association Committee on Energy Statistics. Beginning with the fall 2003 meeting, EIA no longer edits these transcripts. Summaries of previous meetings can be found to the right of the Thursday and Friday transcripts. The public meeting took place April 19 and

  5. Spring 2008 ASA Meeting Disclaimer

    U.S. Energy Information Administration (EIA) Indexed Site

    8 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration In two adjacent files you will find unedited transcripts of EIA's fall 2008 meeting with the American Statistical Association Committee on Energy Statistics. Beginning with the fall 2003 meeting, EIA no longer edits these transcripts. Summaries of previous meetings can be found to the right of the Thursday and Friday transcripts. The public meeting took place October 23 and

  6. Spring 2008 ASA Meeting Disclaimer

    U.S. Energy Information Administration (EIA) Indexed Site

    8 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration In two adjacent files you will find unedited transcripts of EIA's spring 2008 meeting with the American Statistical Association Committee on Energy Statistics. Beginning with the fall 2003 meeting, EIA no longer edits these transcripts. Summaries of previous meetings can be found to the right of the Thursday and Friday transcripts. The public meeting took place on April 9,

  7. Spring 2009 ASA Meeting Disclaimer

    U.S. Energy Information Administration (EIA) Indexed Site

    9 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration In two adjacent files you will find unedited transcripts of EIA's spring 2009 meeting with the American Statistical Association Committee on Energy Statistics. Beginning with the fall 2003 meeting, EIA no longer edits these transcripts. Summaries of previous meetings can be found to the right of the Thursday and Friday transcripts. The public meeting took place on April 2

  8. Untitled Document

    U.S. Energy Information Administration (EIA) Indexed Site

    5 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration In two adjacent files you may find unedited transcripts of EIA's spring 2005 meeting with the American Statistical Association Committee on Energy Statistics. Beginning with the fall 2003 meeting, EIA no longer edits these transcripts. Summaries of previous meetings may be found to the right of the Thursday and Friday transcripts, here at:

  9. State Energy Data System 2014 Consumption Technical Notes

    Gasoline and Diesel Fuel Update (EIA)

    6 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration In two adjacent files you may find unedited transcripts of EIA's spring 2006 meeting with the American Statistical Association Committee on Energy Statistics. Beginning with the fall 2003 meeting, EIA no longer edits these transcripts. Summaries of previous meetings may be found to the right of the Thursday and Friday transcripts. The public meeting took place April 6 and 7,

  10. Fall 2005 Meeting of the ASA

    U.S. Energy Information Administration (EIA) Indexed Site

    6 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration In two adjacent files you may find unedited transcripts of EIA's fall 2006 meeting with the American Statistical Association Committee on Energy Statistics. Beginning with the fall 2003 meeting, EIA no longer edits these transcripts. Summaries of previous meetings may be found to the right of the Thursday and Friday transcripts. The public meeting took place October 6 and 7,

  11. Fall 2007 ASA Meeting Disclaimer

    U.S. Energy Information Administration (EIA) Indexed Site

    7 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration In two adjacent files you will find unedited transcripts of EIA's fall 2007 meeting with the American Statistical Association Committee on Energy Statistics. Beginning with the fall 2003 meeting, EIA no longer edits these transcripts. Summaries of previous meetings can be found to the right of the Thursday and Friday transcripts. The public meeting took place October 18 and

  12. Find Journals

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    | Department of Energy Find Energy Incentives Quicker and Easier with DSIRE Open Data and Website Find Energy Incentives Quicker and Easier with DSIRE Open Data and Website March 3, 2015 - 4:16pm Addthis The updated Database of State Incentives for Renewables & Efficiency helps homeowners and businesses find incentive programs that can reduce or defray installation or purchase costs of technologies like photovoltaic systems. | Photo by Dennis Schroeder, National Renewable Energy

  13. Find Books

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Books Find Books The Library Catalog is available to the public, though many eBooks are limited to LANL researchers. Question? 667-5809 Email Library Catalog: Search here for all...

  14. Find Clear

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    p align"left">

    Find Clear Slide Title Duration Status myMail@adobe.com http:Adobe.com More Info>>

  15. Find Clear

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    p align"left">

    Find Clear Slide Title Duration Status myMail@adobe.com http:Adobe.com More Info>>

  16. Find Reports

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Reports Find Reports Most of the reports sources below are publicly accessible. Question? 667-5809 Email Unclassified Los Alamos Reports Library Catalog - Print and electronic (1943-present) Los Alamos Authors (LANL-only access) - LA-, LA-CP, and LA-UR reports (1973-present) Los Alamos Research Online - Find published versions of Los Alamos research (2006-present) Classified reports - contact the SI-RMS Technical Report Collection for an appointment: phone icon 5-4168 / email icon

  17. Untitled Document

    U.S. Energy Information Administration (EIA) Indexed Site

    4 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration In an adjacent file you may find an unedited transcript of EIA's Fall 2004 meeting with the American Statistical Association Committee on Energy Statistics. Beginning with the fall 2003 meeting, EIA no longer edits these transcripts. The public meeting took place October 28 and 29, 2004 in the Forrestal Building at 1000 Independence Ave., S.W., Washington, D.C. 20585. All of

  18. Find Patents

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Patents Find Patents Some of these resources are Lab-only and will require Remote Access. Questions? 667-5809 Email US Patents Google Patents - search USPTO patents in a Google interface USPTO Database (United States Patent and Trademark Office) DOE Patents - only patents resulting from DOE-sponsored research Worldwide Patents Espacenet Patent Search (European Patent Office) Free Patents Online SciFinder information (from Chemical Abstracts Service) Ordering Patents Thomson Innovation - contact

  19. Find Preprints

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Preprints Find Preprints Journal preprints may only be available via subscription and thus require Remote Access. Questions? 667-5809 Email Databases arXiv.org - open access eprints in Physics, Mathematics, Computer Science, Quantitative Biology, and Statistics bioRxiv.org - preprint server for Biology operated by Cold Spring Harbor Laboratory INSPIRE - High Energy Physics (HEP) papers updated daily (a collaboration of CERN, DESY, Fermilab, and SLAC) Journals Online journals often offer

  20. RNA-guided transcriptional regulation

    DOE Patents [OSTI]

    Church, George M.; Mali, Prashant G.; Esvelt, Kevin M.

    2016-02-23

    Methods of modulating expression of a target nucleic acid in a cell are provided including introducing into the cell a first foreign nucleic acid encoding one or more RNAs complementary to DNA, wherein the DNA includes the target nucleic acid, introducing into the cell a second foreign nucleic acid encoding a nuclease-null Cas9 protein that binds to the DNA and is guided by the one or more RNAs, introducing into the cell a third foreign nucleic acid encoding a transcriptional regulator protein or domain, wherein the one or more RNAs, the nuclease-null Cas9 protein, and the transcriptional regulator protein or domain are expressed, wherein the one or more RNAs, the nuclease-null Cas9 protein and the transcriptional regulator protein or domain co-localize to the DNA and wherein the transcriptional regulator protein or domain regulates expression of the target nucleic acid.

  1. Transcript for Building America Video

    Broader source: Energy.gov [DOE]

    Transcript of a video about how DOE’s Building America program is helping to bridge the gap between homes with high energy costs and homes that are healthy, durable, and energy efficient.

  2. EIS-0458: Scoping Meeting Transcript

    Broader source: Energy.gov [DOE]

    Transcript of the public scoping meeting on the draft EIS for the proposed loan guarantee for the First Solar Topaz Solar Farm Project in San Luis Obispo County, CA.

  3. Find Theses and Dissertations

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Theses and Dissertations Find Theses and Dissertations Most of the theses sources below are publicly accessible. Questions? 667-5809 Email Electronic Databases Open Access Theses and Dissertations - Results limited to freely accessible, full-text ETDs Center for Research Libraries - Foreign Dissertations Collection Theses Canada - Canadian theses from Library and Archives Canada EThOS (Electronic Theses Online Service) - UK theses from the British Library Networked Digital Library of Theses and

  4. Transcription factor-based biosensor

    DOE Patents [OSTI]

    2013-10-08

    The present invention provides for a system comprising a BmoR transcription factor, a .sigma..sup.54-RNA polymerase, and a pBMO promoter operatively linked to a reporter gene, wherein the pBMO promoter is capable of expression of the reporter gene with an activated form of the BmoR and the .sigma..sup.54-RNA polymerase.

  5. Electricity Advisory Committee Meeting, May 20, 2008 (TRANSCRIPT...

    Office of Environmental Management (EM)

    (TRANSCRIPT) Electricity Advisory Committee Meeting, May 20, 2008 (TRANSCRIPT) Transcript of the May 20, 2008 Electricity Advisory Committee Meeting including a FERC presentation ...

  6. Electricity Advisory Committee Meeting, October 29, 2010: Transcript...

    Energy Savers [EERE]

    Transcript Electricity Advisory Committee Meeting, October 29, 2010: Transcript Transcript of the Electricity Advisory Committee Meeting held on October 29, 2010. PDF icon ...

  7. Financing Residential Energy Efficiency with Carbon Offsets Transcript...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Residential Energy Efficiency with Carbon Offsets Transcript Financing Residential Energy Efficiency with Carbon Offsets Transcript This document contains the transcript for the ...

  8. Engaging Financial Institution Partners Transcript.doc | Department...

    Office of Environmental Management (EM)

    Engaging Financial Institution Partners Transcript.doc Engaging Financial Institution Partners Transcript.doc Engaging Financial Institution Partners Transcript.doc Microsoft ...

  9. Transcript from 12/01/08 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    1/08 Transcript from 12/01/08 Transcript from 12/01/08 (14.79 MB) More Documents & Publications Transcript from 12/05/08 Manufacturing Pre-Solicitation Transcript Speaker Bios: Matthew McMillen

  10. Manufacturing Pre-Solicitation Transcript | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Pre-Solicitation Transcript Manufacturing Pre-Solicitation Transcript Transcript from the US DOE Hydrogen Program Manufacturing R&D Pre-Solicitation Meeting pre_solicitation_transcript.pdf (419.1 KB) More Documents & Publications Transcript from 12/05/08 Transcript from 12/01/08 Public Roundtable- Data Access and Privacy Issues Related to Smart Grid Technologies

  11. SERC Photovoltaics for Residential Buildings Webinar Transcript |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Photovoltaics for Residential Buildings Webinar Transcript SERC Photovoltaics for Residential Buildings Webinar Transcript A presentation sponsored by the U.S. Department of Energy about using solar photovoltaics (PV) systems to provide electricity for homes. 20110125_pv_webinar.pdf (109.9 KB) More Documents & Publications Sustainable Energy Resources for Consumers (SERC) - Solar Hot Water Solar Webinar Text Version TAP Webcast Transcript July-29, 2009

  12. Isolated Fungal Promoters and Gene Transcription Terminators...

    Office of Scientific and Technical Information (OSTI)

    The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first ...

  13. Finding of No Significant Impact

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    U.S. Department of Energy Finding of No Significant Impact 2 June 2001 This page intentionally left blank. U.S. Department of Energy Finding of No Significant Impact 12 June 2001 This page intentionally left blank.

  14. ARM - Research Highlights: Notable Findings

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    HighlightsNotable Research Findings Form Submit a New Research Highlight Sort Highlights Submitter Title Research Area Working Group Submission Date DOE Progress Reports Notable Research Findings for 2001-2006 Office of Science Abstracts Database Research Highlights Summaries Notable Research Findings for the Past Five Years Aerosols Cloud Parameterization and Modeling (Currently Cloud Modeling) Cloud Properties Instantaneous Radiative Flux (Currently Radiative Processes)

  15. transcript_pv_mapper.doc | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    transcript_pv_mapper.doc transcript_pv_mapper.doc transcript_pv_mapper.doc transcript_pv_mapper.doc (74.5 KB) More Documents & Publications PVMapper: A Tool for Energy Siting transcript_jedi_model.doc 2009 National Electric Transmission Congestion Study - San Francisco Workshop

  16. Transcript from 12/05/08 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    5/08 Transcript from 12/05/08 Transcript from 12/05/08 (176.91 KB) More Documents & Publications Transcript from 12/01/08 Manufacturing Pre-Solicitation Transcript SERC Photovoltaics for Residential Buildings Webinar Transcript

  17. Telomere-surrounding regions are transcription-permissive 3D nuclear compartments in human cells

    SciTech Connect (OSTI)

    Quina, Ana Sofia; Parreira, Leonor . E-mail: lparreir@igc.gulbenkian.pt

    2005-07-01

    Positioning of genes relative to nuclear heterochromatic compartments is thought to help regulate their transcriptional activity. Given that human subtelomeric regions are rich in highly expressed genes, we asked whether human telomeres are related to transcription-permissive nuclear compartments. To address this question, we investigated in the nuclei of normal human lymphocytes the spatial relations of two constitutively expressed genes (ACTB and RARA) and three nuclear transcripts (ACTB, IL2RA and TCRB) to telomeres and centromeres, as a function of gene activity and transcription levels. We observed that genes and gene transcripts locate close to telomere clusters and away from chromocenters upon activation of transcription. These findings, together with the observation that SC35 domains, which are enriched in pre-mRNA processing factors, are in close proximity to telomeres, indicate that telomere-neighboring regions are permissive to gene expression in human cells. Therefore, the associations of telomeres observed in the interphase nucleus might contribute, as opposed to chromocenters, for the establishment of transcription-permissive 3D nuclear compartments.

  18. Find R&D Results

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Find technical reports, journal articles, accepted manuscripts, conference papers, patents, theses, books, multimedia, and data information. Use the basic or advanced search to get ...

  19. Electricity Advisory Committee Meeting, September 25, 2008: Transcript...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Transcript Electricity Advisory Committee Meeting, September 25, 2008: Transcript Transcript of the Electricity Advisory Committee Meeting 3:30 p.m. through 5:55 p.m on Thursday, ...

  20. TAP Webcast Transcript July-29, 2009

    Broader source: Energy.gov [DOE]

    Transcript of the audio for the July 2009 TAP Webcast titled Economic Impacts of Renewable Energy sponsored by the U.S. Department of Energy (DOE) Technical Assistance Project (TAP) for state and local officials.

  1. Engineering an allosteric transcription factor to respond to...

    Office of Scientific and Technical Information (OSTI)

    Engineering an allosteric transcription factor to respond to new ligands Citation Details In-Document Search Title: Engineering an allosteric transcription factor to respond to new ...

  2. Webinar-Transcript-Repeat.pdf | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Webinar-Transcript-Repeat.pdf Webinar-Transcript-Repeat.pdf (94.89 KB) More Documents & Publications Title Title Slide 1

  3. Find a Job | Careers | NREL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Find a Job We're looking for scientists, research technicians, engineers, analysts, managers and project leaders, administrators, and communicators, along with information technology, security, facilities, and clerical/administrative support specialists. Interested in joining our team? Find your opportunity We're passionate about our mission-to develop clean energy and energy efficiency technologies and practices, advance related science and engineering, and provide knowledge and innovations to

  4. Peak finding using biorthogonal wavelets

    SciTech Connect (OSTI)

    Tan, C.Y.

    2000-02-01

    The authors show in this paper how they can find the peaks in the input data if the underlying signal is a sum of Lorentzians. In order to project the data into a space of Lorentzian like functions, they show explicitly the construction of scaling functions which look like Lorentzians. From this construction, they can calculate the biorthogonal filter coefficients for both the analysis and synthesis functions. They then compare their biorthogonal wavelets to the FBI (Federal Bureau of Investigations) wavelets when used for peak finding in noisy data. They will show that in this instance, their filters perform much better than the FBI wavelets.

  5. Department of Energy (DOE) Webinar Transcript | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    (DOE) Webinar Transcript Department of Energy (DOE) Webinar Transcript Department of Energy (DOE) Webinar Transcript (95.26 KB) More Documents & Publications Welcome to the Department of Energy’s Recovery Act webinar on reporting in to federalreporting Department of Energy (DOE) Webinar Transcript Registration Overview for OMB Section 1512 Reporting for DOE Prime Recipients

  6. Transcription factor-based biosensors for detecting dicarboxylic acids

    DOE Patents [OSTI]

    Dietrich, Jeffrey; Keasling, Jay

    2014-02-18

    The invention provides methods and compositions for detecting dicarboxylic acids using a transcription factor biosensor.

  7. Writing audit findings: Be reasonable!

    SciTech Connect (OSTI)

    Girvin, N.W.

    1992-05-01

    A customary approach to auditing and reporting deficiencies is to keep a running list of those that are found, evaluate the severity of each, and based on the evidence, document findings or observations or concerns in an audit report. The report is issued and the auditee is normally requested to address ``root cause`` as part of their corrective action. This paper describes a ``root problems`` approach to documenting audit findings that is designed not only to put the QA auditor in a more favorable light, but to more effectively enable the auditee to identify root cause and meaningful corrective action. The positive results of this approach are considerable. You will have fewer findings but those you do have will be substantial. You will cite requirements that sound reasonable and make arguments difficult. If some of the supporting deficiencies (examples) prove to be incorrect, you will still have ample support for the original finding. You will be seen as reasonable individual who can help lead the auditee towards identification of root cause without taking away part of the responsibility. You even have a fair chance of fostering a sense of commitment to quality improvement on the auditee`s part. This in itself, is its own reward.

  8. Writing audit findings: Be reasonable

    SciTech Connect (OSTI)

    Girvin, N.W.

    1992-05-01

    A customary approach to auditing and reporting deficiencies is to keep a running list of those that are found, evaluate the severity of each, and based on the evidence, document findings or observations or concerns in an audit report. The report is issued and the auditee is normally requested to address root cause'' as part of their corrective action. This paper describes a root problems'' approach to documenting audit findings that is designed not only to put the QA auditor in a more favorable light, but to more effectively enable the auditee to identify root cause and meaningful corrective action. The positive results of this approach are considerable. You will have fewer findings but those you do have will be substantial. You will cite requirements that sound reasonable and make arguments difficult. If some of the supporting deficiencies (examples) prove to be incorrect, you will still have ample support for the original finding. You will be seen as reasonable individual who can help lead the auditee towards identification of root cause without taking away part of the responsibility. You even have a fair chance of fostering a sense of commitment to quality improvement on the auditee's part. This in itself, is its own reward.

  9. Find Internships | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Internships Find Internships Internships in energy efficiency and renewable energy-related fields, such as solar and fuel cells, energy efficiency, atmospheric sciences, ecology, global carbon cycles, climatology, and more, include EERE's own student internship program. Featured Internships Clean Cities University Workforce Development Program Clean Cities logo Science Undergraduate Laboratory Internships (SULI) Fall 2013 Picture of tow people in a labratory working Minority Educational

  10. FINDING OF NO SIGNIFICANT IMPACT

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    COMMERCIAL DEMONSTRATION OF THE MANUFACTURED AGGREGATE PROCESSING TECHNOLOGY UTILIZING SPRAY DRYER ASH 1 AGENCY: U.S. Department of Energy (DOE) ACTION: Finding of No Significant Impact (FONSI) SUMMARY: The DOE has prepared an Environmental Assessment (EA), DOE/EA-1449, to analyze the potential environmental consequences of participating in a cooperative agreement with Universal Aggregates, LLC, for the design, construction, and operation of a plant to manufacture lightweight aggregates. The

  11. Find Jobs | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Jobs Find Jobs Clean energy jobs can be found in the public, private, and nonprofit sectors and can range from entry-level to professional positions in a wide range of fields. This section can help you familiarize yourself with different types of green energy jobs as you begin your search for a job that meets your interests and skills. EERE Career Opportunities EERE Career Opportunities Energy Efficency and Renewable Energy, the DOE office leading the nation's effort to enhance energy efficiency

  12. Transcriptional enhancer from milk protein genes

    DOE Patents [OSTI]

    Casperson, Gerald F.; Schmidhauser, Christian T.; Bissell, Mina J.

    1999-01-01

    The invention relates to novel enhancer nucleotide sequences which stimulate transcription of heterologous DNA in cells in culture. The enhancers are derived from major milk protein genes by the process of deletion mapping and functional analysis. The invention also relates to expression vectors containing the novel enhancers.

  13. Method to determine transcriptional regulation pathways in organisms

    DOE Patents [OSTI]

    Gardner, Timothy S.; Collins, James J.; Hayete, Boris; Faith, Jeremiah

    2012-11-06

    The invention relates to computer-implemented methods and systems for identifying regulatory relationships between expressed regulating polypeptides and targets of the regulatory activities of such regulating polypeptides. More specifically, the invention provides a new method for identifying regulatory dependencies between biochemical species in a cell. In particular embodiments, provided are computer-implemented methods for identifying a regulatory interaction between a transcription factor and a gene target of the transcription factor, or between a transcription factor and a set of gene targets of the transcription factor. Further provided are genome-scale methods for predicting regulatory interactions between a set of transcription factors and a corresponding set of transcriptional target substrates thereof.

  14. HIV transcription is induced in dying cells

    SciTech Connect (OSTI)

    Woloschak, G.E.; Chang-Liu, Chin-Mei; Schreck, S. |; Panozzo, J.; Libertin, C.R.

    1996-02-01

    Using HeLa cells stably transfected with an HIV-LTR-CAT construct, we demonstrated a peak in CAT induction that occurs in viable (but not necessarily cell-division-competent) cells 24 h following exposure to some cell-killing agents. {gamma} rays were the only cell-killing agent which did not induce HIV transcription; this can be attributed to the fact that {gamma}-ray-induced apoptotic death requires functional p53, which is not present in HeLa cells. For all other agents, HIV-LTR induction was dose-dependent and correlated with the amount of cell killing that occurred in the culture. Doses which caused over 99% cell killing induced HIV-LTR transcription maximally, demonstrating that cells that will go on to die by 14 days are the cells expressing HIV-LTR-CAT.

  15. Blackout 2003: Transcript of Technical Conference with the Electric System

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Investigation Team | Department of Energy Transcript of Technical Conference with the Electric System Investigation Team Blackout 2003: Transcript of Technical Conference with the Electric System Investigation Team Electric System Investigation Team: 8 Reliability Recommendation consultation Transcript of the technical conference sponsored by the U.S. Canada task force investigating the August 14, 2003 blackout across Canada and the Northeastern United States. The transcript includes reports

  16. Public Meeting Transcript: Sustainable Design Standards for Federal Buildings

    Broader source: Energy.gov [DOE]

    This transcript is from the public meeting to discuss sustainable design standards for Federal buildings held on July 28, 2010.

  17. csep_transcript_transportation.doc | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    More Documents & Publications cseptranscriptminneapolis.doc Marketing & Driving Demand: Social Media Tools & Strategies - January 16, 2011 (Text Version) Transcript: Biomass ...

  18. 20130226_sunshot_transcript_lbnl.doc | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    transcript_lbnl.doc 20130226_sunshot_transcript_lbnl.doc 20130226_sunshot_transcript_lbnl.doc 20130226_sunshot_transcript_lbnl.doc (73.5 KB) More Documents & Publications Why Are Resiential PV Prices in Germany So Much Lower Than in the United States? text_alternative_stat_webinar_2012_7_25.docx An Analysis of Residential PV System Price Differences between the United States and Germany

  19. Financing Residential Energy Efficiency with Carbon Offsets Transcript

    Broader source: Energy.gov [DOE]

    This document contains the transcript for the Financing Residential Energy Efficiency with Carbon Offsets webinar held on March 12, 2013.

  20. CSC Transcript of Information Session | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Transcript of Information Session CSC Transcript of Information Session CSC Transcript of Information Session (2.04 MB) More Documents & Publications CSC Notice of Public Meeting Convention on Supplementary Compensation Rulemaking Enforcement Notice of Intent to Investigate, Computer Sciences Corporation

  1. CSC Workshop Transcript 02-20-2105 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Workshop Transcript 02-20-2105 CSC Workshop Transcript 02-20-2105 CSC Workshop Transcript 02-20-2105 (687.86 KB) More Documents & Publications EA-1971: FERC Notice of Intent to Prepare an Environmental Assessment EIS-0501: Notice of Intent Testimony

  2. Regional Transmission Projects: Finding Solutions

    SciTech Connect (OSTI)

    The Keystone Center

    2005-06-15

    The Keystone Center convened and facilitated a year-long Dialogue on "Regional Transmission Projects: Finding Solutions" to develop recommendations that will help address the difficult and contentious issues related to expansions of regional electric transmission systems that are needed for reliable and economic transmission of power within and across regions. This effort brought together a cross-section of affected stakeholders and thought leaders to address the problem with the collective wisdom of their experience and interests. Transmission owners sat at the table with consumer advocates and environmental organizations. Representatives from regional transmission organizations exchanged ideas with state and federal regulators. Generation developers explored common interests with public power suppliers. Together, the Dialogue participants developed consensus solutions about how to begin unraveling some of the more intractable issues surrounding identification of need, allocation of costs, and reaching consensus on siting issues that can frustrate the development of regional transmission infrastructure. The recommendations fall into three broad categories: 1. Recommendations on appropriate institutional arrangements and processes for achieving regional consensus on the need for new or expanded transmission infrastructure 2. Recommendations on the process for siting of transmission lines 3. Recommendations on the tools needed to support regional planning, cost allocation, and siting efforts. List of Dialogue participants: List of Dialogue Participants: American Electric Power American Transmission Company American Wind Energy Association California ISO Calpine Corporation Cinergy Edison Electric Institute Environmental Defense Federal Energy Regulatory Commission Great River Energy International Transmission Company ISO-New England Iowa Public Utility Board Kanner & Associates Midwest ISO National Association of Regulatory Utility Commissioners National Association

  3. Finding Hidden Oil and Gas Reserves

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Finding Hidden Oil and Gas Reserves Finding Hidden Oil and Gas Reserves Key Challenges: Seismic imaging methods, vital in our continuing search for deep offshore oil and gas...

  4. Canfield's Couplet Clues for Finding Superconductors

    ScienceCinema (OSTI)

    Canfield, Paul

    2015-06-30

    Ames Laboratory scientist, Paul Canfield, summarizes his thoughts about finding new superconducting materials.

  5. Canfield's Couplet Clues for Finding Superconductors

    SciTech Connect (OSTI)

    Canfield, Paul

    2015-04-28

    Ames Laboratory scientist, Paul Canfield, summarizes his thoughts about finding new superconducting materials.

  6. June 2014 Electricity Advisory Committee Meeting Summaries and Transcripts

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Now Available | Department of Energy 2014 Electricity Advisory Committee Meeting Summaries and Transcripts Now Available June 2014 Electricity Advisory Committee Meeting Summaries and Transcripts Now Available August 13, 2014 - 11:17am Addthis The meeting summaries and transcripts for the Electricity Advisory Committee (EAC) meeting held on June 16-17, 2014, are now available. The EAC provides advice to the U.S. Department of Energy in implementing the Energy Policy Act of 2005, executing

  7. Reducing nontemplated 3' nucleotide addition to polynucleotide transcripts

    DOE Patents [OSTI]

    Kao, C. Cheng

    2000-01-01

    Non-template 3' nucleotide addition to a transcript is reduced by transcribing a transcript from a template comprising an ultimate and/or penultimate 5' ribose having a C'2 substituent such as methoxy, which reduces non-template 3' nucleotide addition to the transcript. The methods are shown to be applicable to a wide variety of polymerases, including Taq, T7 RNA polymerase, etc.

  8. Unlocking the Mysteries of the J. Robert Oppenheimer Transcript |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy the Mysteries of the J. Robert Oppenheimer Transcript Unlocking the Mysteries of the J. Robert Oppenheimer Transcript October 3, 2014 - 2:48pm Addthis This is an excerpt from the original press release from the Atomic Energy Commission announcing the publication of the redacted Oppenheimer transcript. | Photo courtesy of the Energy Department. This is an excerpt from the original press release from the Atomic Energy Commission announcing the publication of the redacted

  9. HIV transcription is induced in dying cells

    SciTech Connect (OSTI)

    Woloschak, G.E.; Chang-Liu, Chin-Mei; Schreck, S. |

    1995-06-01

    Using HeLa cells stably transfected with an HIV-LTR-CAT construct, we demonstrated a peak in CAT induction that occurs in viable (but not necessarily cell-division-competent) cells 24 h following exposure to some cell-killing agents. {gamma} rays were the only cell-killing agent which did not induce HIV transcription; this can be attributed to the fact that {gamma}-ray-induced apoptotic death requires functional p53, which is not present in HeLa cells. For all other agents, HIV-LTR induction was dose-dependent and correlated with the amount of cell killing that occurred in the culture. 14 refs., 4 figs., 1 tab.

  10. transcript_jedi_model.doc | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    document icon transcriptjedimodel.doc More Documents & Publications TAP Webcast Transcript July-29, 2009 Scenario Jedi Tools for Designing & Implementing Better Finance Programs

  11. Transcript: OSTI Highlights First-Time Electronic Availability...

    Office of Scientific and Technical Information (OSTI)

    Transcript: OSTI Highlights First-Time Electronic Availability of Geothermal Documents at San Diego Geothermal Conference, Office of Scientific and Technical Information, U.S. ...

  12. Roger D. Kornberg Polymerase, DNA, RNA, and Transcription

    Office of Scientific and Technical Information (OSTI)

    Kornberg's studies have provided an understanding at the atomic level of how the process of transcription occurs and also how it is controlled. Because the regulation of ...

  13. Transcript of March 28, 2013, TAP webinar titled Internal Benchmarking...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    TAP webinar titled Internal Benchmarking Outreach and Data Collection Techniques Transcript of March 28, 2013, TAP webinar titled Internal Benchmarking Outreach and Data ...

  14. Blackout 2003: Transcript of Technical Conference with the Electric...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Transcript of the technical conference sponsored by the U.S. Canada task force investigating the August 14, 2003 blackout across Canada and the Northeastern United States. The ...

  15. TE Connectivity Finds Answers in Tomography

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    TE Connectivity Finds Answers in Tomography TE Connectivity Finds Answers in Tomography Print Thursday, 22 August 2013 10:50 TE Connectivity is a world leader in connectivity-the...

  16. Transcription factor FOXA2-centered transcriptional regulation network in non-small cell lung cancer

    SciTech Connect (OSTI)

    Jang, Sang-Min; An, Joo-Hee; Kim, Chul-Hong; Kim, Jung-Woong Choi, Kyung-Hee

    2015-08-07

    Lung cancer is the leading cause of cancer-mediated death. Although various therapeutic approaches are used for lung cancer treatment, these mainly target the tumor suppressor p53 transcription factor, which is involved in apoptosis and cell cycle arrest. However, p53-targeted therapies have limited application in lung cancer, since p53 is found to be mutated in more than half of lung cancers. In this study, we propose tumor suppressor FOXA2 as an alternative target protein for therapies against lung cancer and reveal a possible FOXA2-centered transcriptional regulation network by identifying new target genes and binding partners of FOXA2 by using various screening techniques. The genes encoding Glu/Asp-rich carboxy-terminal domain 2 (CITED2), nuclear receptor subfamily 0, group B, member 2 (NR0B2), cell adhesion molecule 1 (CADM1) and BCL2-associated X protein (BAX) were identified as putative target genes of FOXA2. Additionally, the proteins including highly similar to heat shock protein HSP 90-beta (HSP90A), heat shock 70 kDa protein 1A variant (HSPA1A), histone deacetylase 1 (HDAC1) and HDAC3 were identified as novel interacting partners of FOXA2. Moreover, we showed that FOXA2-dependent promoter activation of BAX and p21 genes is significantly reduced via physical interactions between the identified binding partners and FOXA2. These results provide opportunities to understand the FOXA2-centered transcriptional regulation network and novel therapeutic targets to modulate this network in p53-deficient lung cancer. - Highlights: • Identification of new target genes of FOXA2. • Identifications of novel interaction proteins of FOXA2. • Construction of FOXA2-centered transcriptional regulatory network in non-small cell lung cancer.

  17. Department of Energy (DOE) Webinar Transcript | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    Department of Energy (DOE) Webinar Transcript (145.56 KB) More Documents & Publications Welcome to the Department of Energy’s Recovery Act webinar on reporting in to federalreporting Department of Energy (DOE) Webinar Transcript Registration Overview for OMB Section 1512 Reporting for DOE Prime Recipients

  18. Fall Lectures Feature Life of Einstein; Exploring Our World With Particle

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    5 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration In two adjacent files are the unedited transcripts of EIA's fall 2005 meeting with the American Statistical Association Committee on Energy Statistics. Beginning with the fall 2003 meeting, EIA no longer edits these transcripts. Summaries of previous meetings may be found to the right of the Thursday and Friday transcripts, here at:

  19. Fall 2005 Meeting of the ASA

    U.S. Energy Information Administration (EIA) Indexed Site

    5 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration In two adjacent files are the unedited transcripts of EIA's fall 2005 meeting with the American Statistical Association Committee on Energy Statistics. Beginning with the fall 2003 meeting, EIA no longer edits these transcripts. Summaries of previous meetings may be found to the right of the Thursday and Friday transcripts, here at:

  20. ORISE: Forensic scientist finds truth in fingerprints

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Forensic scientist finds truth in fingerprints For more than 30 years, Imogene Van Buren has searched for truth in the friction ridges of fingerprints. She has identified victims...

  1. Right rock: Finding/refining customer expectations

    SciTech Connect (OSTI)

    Ashby, R.

    1997-11-01

    This report contains viewgraphs on methods of finding customer expectations of software and then refining the documentation and software to fit these expectations.

  2. Microsoft Word - Consolidated Verbatim Transcript CBS Meeting Sacramento California Transcripts_Final

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Holiday Inn Capitol Plaza Sacramento 300 J Street Sacramento, CA 95814 April 26, 2016 VERBATIM TRANSCRIPT Mr. Jim Hamilton. Good afternoon. And for those in later time zones via webinar, good evening. Welcome, and thank you for being here today. My name is Jim Hamilton. I'm an advisor to the Department of Energy's Consent-Based Siting Team and my role here today is to help us all to have a good, open, productive conversation. To start off we have a few housekeeping issues I want to talk about

  3. Transcriptional bursting explains the noise–versus–mean relationship in mRNA and protein levels

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Dar, Roy; Shaffer, Sydney M.; Singh, Abhyudai; Razooky, Brandon S.; Simpson, Michael L.; Raj, Arjun; Weinberger, Leor S.

    2016-07-28

    Recent analysis demonstrates that the HIV-1 Long Terminal Repeat (HIV LTR) promoter exhibits a range of possible transcriptional burst sizes and frequencies for any mean-expression level. However, these results have also been interpreted as demonstrating that cell-tocell expression variability (noise) and mean are uncorrelated, a significant deviation from previous results. Here, we re-examine the available mRNA and protein abundance data for the HIV LTR and find that noise in mRNA and protein expression scales inversely with the mean along analytically predicted transcriptional burst-size manifolds. We then experimentally perturb transcriptional activity to test a prediction of the multiple burst-size model: thatmore » increasing burst frequency will cause mRNA noise to decrease along given burst-size lines as mRNA levels increase. In conclusion, the data show that mRNA and protein noise decrease as mean expression increases, supporting the canonical inverse correlation between noise and mean.« less

  4. Public Meeting Transcript: Sustainable Design Standards for Federal...

    Broader source: Energy.gov (indexed) [DOE]

    This transcript is from the public meeting to discuss sustainable design standards for Federal buildings held on July 28, 2010. noprmeeting072810.pdf (345.56 KB) More Documents & ...

  5. Transcript, Keeping the Edge: Enhancing Performance by Managing Culture

    Broader source: Energy.gov [DOE]

    Transcript: 2003 INPO CEO Conference, Keeping the Edge: “Enhancing Performance by Managing Culture” Edgar H. Schein, Ph.D. Professor Emeritis, Massachusetts Institute of Technology November 7, 2003

  6. National Electric Transmission Congestion Study: Preliminary Findings

    Broader source: Energy.gov [DOE]

    The Department hosted three webinars in August 2012 to receive input and suggestions concerning the preliminary findings of the National Electric Transmission Congestion Study. The updated presentation used in the webinars is now available.

  7. Missourian Finds New Opportunity in Energy Industry

    Broader source: Energy.gov [DOE]

    How one Missouri man decided to take matters into his own hands - creating a company that improves energy efficiency in homes and buildings - after not being able to find work during the recession.

  8. NREL Publishes Gearbox Reliability Collaborative Findings Report |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Publishes Gearbox Reliability Collaborative Findings Report NREL Publishes Gearbox Reliability Collaborative Findings Report October 3, 2011 - 12:56pm Addthis This is an excerpt from the Third Quarter 2011 edition of the Wind Program R&D Newsletter. In June, DOE's National Renewable Energy Laboratory (NREL) published the first formal report on the efforts of the Gearbox Reliability Collaborative (GRC) to address a major challenge for the wind energy industry-gearbox

  9. Mars Rover finds changing rocks, surprising scientists

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Mars Rover finds changing rocks, surprising scientists Mars Rover finds changing rocks, surprising scientists As NASA's Curiosity rover treks up a three-mile-high mountain on Mars, the rocks are changing. Back on Earth, scientists analyzing the data realized this was something different: It turned out to be the first of the high-silica rocks. December 24, 2015 Mars landscape This color-adjusted composite of images taken by NASA's Curiosity rover in September shows the lower portion of Mount

  10. Floodplain Assessment and Statement of Findings

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    and Statement of Findings for Installation of a Renewable Energy Anaerobic Digester Facility at the University of California, Davis in Yolo County, California January 28, 2013 1.0 Introduction This Floodplain Assessment and Statement of Findings has been prepared in accordance with 10 Code of Federal Regulations (CFR) 1022, "Compliance with Floodplain and Wetland Environmental Review Requirements" which were promulgated to implement the requirements of the U.S. Department of Energy's

  11. Floodplain Statement of Findings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Floodplain Statement of Findings Floodplain Statement of Findings Loan Guarantee to AES Energy Storage, LLC Project Dyno Electric Grid Stability Advanced Battery Systems Project Dyno would provide ancillary services within the New York power market. The Project is composed of advanced lithium ion battery cells and power control technologies to store power to help maintain the stability of the electric power grid. The Project would be composed of ten fifty-three foot metal containers each housing

  12. Distributed PV Interconnection Recent Analysis Findings

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Recent Analysis Findings Page 1 of 18 Kristen Ardani, Miriam Makhyoun Page 1 of 18 [Speaker: Kristen Ardani] Cover Slide: Good afternoon, everyone. Thank you for joining the Distributed Generation Interconnection Collaborative informational webinar. Today, we are kicking off 2015 with a joint presentation from SEPA and NREL, in which each will discuss recent research and analysis findings related to interconnection. Slide 2: So really, the purpose of today's meeting is to hear recent research

  13. Distributed PV Interconnection: Recent Analysis Findings

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    January 21, 2015 "NREL and SEPA Recent Analysis Findings" Miriam Makhyoun, Solar Electric Power Association (SEPA) Kristen Ardani, National Renewable Energy Laboratory (NREL) 2 Purpose of Today's Meeting * Hear results from SEPA's recent survey of utility interconnection practices. o SEPA finds that utilities confront common challenges as they move towards more streamlined interconnection application processing. * Hear NREL results of forthcoming DGIC data collection and analysis,

  14. Jefferson Laboratory Findings Excite Theoreticians, Experimentatlists |

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Jefferson Lab Laboratory Findings Excite Theoreticians, Experimentatlists Jefferson Laboratory Findings Excite Theoreticians, Experimentatlists March 1999 Seemingly reasonable assumptions define the human view of the world. But ruled of thumb can mislead - or be altogether incorrect. Fortunately, in physics, most assumptions can be tested. Those that don't measure up are amended. An experiment at the Department of Energy's Thomas Jefferson National Accelerator Facility (Jefferson Lab) has

  15. TE Connectivity Finds Answers in Tomography

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    TE Connectivity Finds Answers in Tomography TE Connectivity Finds Answers in Tomography Print Thursday, 22 August 2013 10:50 TE Connectivity is a world leader in connectivity-the $13 billion global company designs and manufactures more than 500,000 different electronic connectivity products for the automotive, energy, industrial, broadband communications, consumer device, healthcare, aerospace, and defense industries. TE Connectivity has a long-standing commitment to innovation and engineering

  16. TE Connectivity Finds Answers in Tomography

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    TE Connectivity Finds Answers in Tomography TE Connectivity Finds Answers in Tomography Print Thursday, 22 August 2013 10:50 TE Connectivity is a world leader in connectivity-the $13 billion global company designs and manufactures more than 500,000 different electronic connectivity products for the automotive, energy, industrial, broadband communications, consumer device, healthcare, aerospace, and defense industries. TE Connectivity has a long-standing commitment to innovation and engineering

  17. Common Errors and Innovative Solutions Transcript | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Common Errors and Innovative Solutions Transcript Common Errors and Innovative Solutions Transcript An example of case studies, mainly by showing photos of errors and good examples, then discussing the purpose of the home energy professional guidelines and certification. There may be more examples of what not to do only because these were good learning opportunities. common_errors_innovative_solutions.doc (41.5 KB) More Documents & Publications WIPP Weatherization: Common Errors and

  18. Roger D. Kornberg Polymerase, DNA, RNA, and Transcription

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Roger D. Kornberg Polymerase, DNA, RNA, and Transcription Resources with Additional Information Roger D. Kornberg Credit: Linda A. Cicero/ Stanford News Service Roger D. Kornberg was awarded the 2006 Nobel Prize in Chemistry "for his studies of the molecular basis of eukaryotic transcription". He determined 'how DNA's genetic blueprint is read and used to direct the process for protein manufacture. Kornberg carried out a significant part of the research leading to this prize at the

  19. Transcription Factors Bind Thousands of Active and InactiveRegions in the Drosophila Blastoderm

    SciTech Connect (OSTI)

    Li, Xiao-Yong; MacArthur, Stewart; Bourgon, Richard; Nix, David; Pollard, Daniel A.; Iyer, Venky N.; Hechmer, Aaron; Simirenko, Lisa; Stapleton, Mark; Luengo Hendriks, Cris L.; Chu, Hou Cheng; Ogawa, Nobuo; Inwood, William; Sementchenko, Victor; Beaton, Amy; Weiszmann, Richard; Celniker, Susan E.; Knowles, David W.; Gingeras, Tom; Speed, Terence P.; Eisen, Michael B.; Biggin, Mark D.

    2008-01-10

    Identifying the genomic regions bound by sequence-specific regulatory factors is central both to deciphering the complex DNA cis-regulatory code that controls transcription in metazoans and to determining the range of genes that shape animal morphogenesis. Here, we use whole-genome tiling arrays to map sequences bound in Drosophila melanogaster embryos by the six maternal and gap transcription factors that initiate anterior-posterior patterning. We find that these sequence-specific DNA binding proteins bind with quantitatively different specificities to highly overlapping sets of several thousand genomic regions in blastoderm embryos. Specific high- and moderate-affinity in vitro recognition sequences for each factor are enriched in bound regions. This enrichment, however, is not sufficient to explain the pattern of binding in vivo and varies in a context-dependent manner, demonstrating that higher-order rules must govern targeting of transcription factors. The more highly bound regions include all of the over forty well-characterized enhancers known to respond to these factors as well as several hundred putative new cis-regulatory modules clustered near developmental regulators and other genes with patterned expression at this stage of embryogenesis. The new targets include most of the microRNAs (miRNAs) transcribed in the blastoderm, as well as all major zygotically transcribed dorsal-ventral patterning genes, whose expression we show to be quantitatively modulated by anterior-posterior factors. In addition to these highly bound regions, there are several thousand regions that are reproducibly bound at lower levels. However, these poorly bound regions are, collectively, far more distant from genes transcribed in the blastoderm than highly bound regions; are preferentially found in protein-coding sequences; and are less conserved than highly bound regions. Together these observations suggest that many of these poorly-bound regions are not involved in early

  20. Finding a Career in Energy Efficiency

    Broader source: Energy.gov [DOE]

    Kendra went to a county agency hoping they could help her find work, but what she really wanted was a career. That’s when she was referred to an upcoming weatherization training program hosted by the Laborers’ International Union of North America, a half-million strong labor union for construction workers, including weatherization workers.

  1. An interesting finding in sup 229 Th

    SciTech Connect (OSTI)

    Reich, C.W.

    1990-01-01

    Work at INEL has recently established that the first excited states of {sup 229}Th forms a closely spaced doublet with the ground state, the separation being 1 {plus minus} 4 electron volts. A discussion of the data and the reasoning supporting this unprecedented finding is given. Some potential applications are briefly mentioned. 27 refs., 3 figs., 2 tabs.

  2. Resources for Handling Transcripts | U.S. DOE Office of Science (SC)

    Office of Science (SC) Website

    Graduate Transcripts for Current Graduate Institution » Resources for Handling Transcripts DOE Office of Science Graduate Student Research (SCGSR) Program SCGSR Home Eligibility Benefits Participant Obligations How to Apply Identifying a Collaborating DOE Laboratory Scientist Research Proposal Guidelines Office of Science Priority Research Areas for SCGSR Program Letters of Support Graduate Transcripts for Current Graduate Institution Resources for Handling Transcripts Application Evaluation

  3. Electricity Advisory Committee Meeting, January 24, 2014- Meeting Summary and Transcript

    Broader source: Energy.gov [DOE]

    Meeting summary and transcript for the January 24, 2014 meeting of the Electricity Advisory Committee.

  4. Meeting of the Electricity Advisory Committee, June 1 & 2, 2016- Meeting Minutes and Transcripts

    Office of Energy Efficiency and Renewable Energy (EERE)

    Meeting minutes and transcripts for the June 1 & 2, 2016 meeting of the Electricity Advisory Committee.

  5. Energy Savings Performance Contracting-Savings Measurement and Verification Transcript 2-24-2011.doc

    Broader source: Energy.gov [DOE]

    Energy Savings Performance Contracting-Savings Measurement and Verification Transcript 2-24-2011.doc

  6. Transcript of the Guidelines for Home Energy Professionals project Prezi presentation.

    Broader source: Energy.gov [DOE]

    This document contains the transcript of the Guidelines for Home Energy Professionals project Prezi presentation.

  7. Electricity Advisory Committee Meeting, March 10, 2011- Meeting Minutes and Transcript

    Broader source: Energy.gov [DOE]

    Meeting minutes and transcript for the March 10, 2011 meeting of the Electricity Advisory Committee.

  8. Community Development Finance Institutions-Opportunities for Partnerships with Energy Efficiency Programs Transcript.doc

    Office of Energy Efficiency and Renewable Energy (EERE)

    Community Development Finance Institutions-Opportunities for Partnerships with Energy Efficiency Programs Transcript.doc

  9. Identification and characterization of transcription networks in environmentally significant species

    SciTech Connect (OSTI)

    Lawrence, Charles E.; McCue, Lee Ann

    2005-11-30

    Understanding the regulation of gene expression, transcription regulation in particular, is one of the grand challenges of molecular biology. Transcription regulation is arguably the most important foundation of cellular function, since it exerts the most fundamental control of the abundance of virtually all of a cell's functional macromolecules. Nevertheless, this process, perhaps because of its difficulty, has been the subject of only a limited number of genomic level analyses. We have undertaken bioinformatics projects to address this issue by developing (1) a cross-species comparison method (i.e. phylogenetic footprinting) for the identification of transcription factor binding sites, (2) a Bayesian clustering method to identify regulons, (3) an improved scanning algorithm that uses a position weight matrix and several related species sequence data to locate transcription factor binding sites, and (4) a method to predict cognate binding sites for transcription factors of unknown specificity. These bioinformatics methods were developed using the model proteobacterium Escherichia coli, with further applications to the genomes of environmentally significant microbes (Rhodopseudomonas palustris, Shewanella oneidensis) in later years of the grant.

  10. Lab supercomputer finds new home at UNM

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Lab supercomputer to UNM Community Connections: Your link to news and opportunities from Los Alamos National Laboratory Latest Issue: September 1, 2016 all issues All Issues » submit Lab supercomputer finds new home at UNM Chance of a lifetime for students with complex research projects March 1, 2015 UNM logo. UNM logo. Contact Community Programs Director Kurt Steinhaus Email Editor Ute Haker Email The New Mexico Consortium has donated Los Alamos National Laboratory's Ulam supercomputer to the

  11. Occupancy by key transcription factors is a more accurate predictor of enhancer activity than histone modifications or chromatin accessibility

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Dogan, Nergiz; Wu, Weisheng; Morrissey, Christapher S.; Chen, Kuan-Bei; Stonestrom, Aaron; Long, Maria; Keller, Cheryl A.; Cheng, Yong; Jain, Deepti; Visel, Axel; et al

    2015-04-23

    Regulated gene expression controls organismal development, and variation in regulatory patterns has been implicated in complex traits. Thus accurate prediction of enhancers is important for further understanding of these processes. Genome-wide measurement of epigenetic features, such as histone modifications and occupancy by transcription factors, is improving enhancer predictions, but the contribution of these features to prediction accuracy is not known. Given the importance of the hematopoietic transcription factor TAL1 for erythroid gene activation, we predicted candidate enhancers based on genomic occupancy by TAL1 and measured their activity. Contributions of multiple features to enhancer prediction were evaluated based on the resultsmore » of these and other studies. Results: TAL1-bound DNA segments were active enhancers at a high rate both in transient transfections of cultured cells (39 of 79, or 56%) and transgenic mice (43 of 66, or 65%). The level of binding signal for TAL1 or GATA1 did not help distinguish TAL1-bound DNA segments as active versus inactive enhancers, nor did the density of regulation-related histone modifications. A meta-analysis of results from this and other studies (273 tested predicted enhancers) showed that the presence of TAL1, GATA1, EP300, SMAD1, H3K4 methylation, H3K27ac, and CAGE tags at DNase hypersensitive sites gave the most accurate predictors of enhancer activity, with a success rate over 80% and a median threefold increase in activity. Chromatin accessibility assays and the histone modifications H3K4me1 and H3K27ac were sensitive for finding enhancers, but they have high false positive rates unless transcription factor occupancy is also included. Conclusions: Occupancy by key transcription factors such as TAL1, GATA1, SMAD1, and EP300, along with evidence of transcription, improves the accuracy of enhancer predictions based on epigenetic features.« less

  12. Nuclear sequestration of COL1A1 mRNA transcript associated with type I osteogenesis imperfecta (OI)

    SciTech Connect (OSTI)

    Primorac, D.; Stover, M.L.; McKinstry, M.B.

    1994-09-01

    Previously we identified an OI type I patient with a splice donor mutation that resulted in intron 26 retention instead of exon skipping and sequestration of normal levels of the mutant transcript in the nuclear compartment. Intron retention was consistent with the exon definition hypothesis for splice site selection since the size of the exon-intron-exon unit was less than 300 bp. Furthermore, the retained intron contained in-frame stop codons which is thought to cause the mutant RNA to remain within the nucleus rather than appearing in the cytoplasm. To test these hypotheses, genomic fragments containing the normal sequence or the donor mutation were cloned into a collagen minigene and expressed in stably tansfected NIH 3T3 cells. None of the modifications to the normal intron altered the level of RNA that accumulated in the cytoplasm, as expected. However none of the modifications to the mutant intron allowed accumulation of normal levels of mRNA in the cytoplasm. Moreover, in contrast to our findings in the patient`s cells only low levels of mutant transcript were found in the nucleus; a fraction of the transcript did appear in the cytoplasm which had spliced the mutant donor site correctly. Nuclear run-on experiments demonstrated equal levels of transcription from each transgene. Expression of another donor mutation known to cause in-frame exon skipping in OI type IV was accurately reproduced in the minigene in transfected 3T3 cells. Our experience suggests that either mechanism can lead to formation of a null allele possibly related to the type of splicing events surrounding the potential stop codons. Understanding the rules governing inactivation of a collagen RNA transcript may be important in designing a strategy to inactivate a dominate negative mutation associated with the more severe forms of OI.

  13. Occupancy by key transcription factors is a more accurate predictor of enhancer activity than histone modifications or chromatin accessibility

    SciTech Connect (OSTI)

    Dogan, Nergiz; Wu, Weisheng; Morrissey, Christapher S.; Chen, Kuan-Bei; Stonestrom, Aaron; Long, Maria; Keller, Cheryl A.; Cheng, Yong; Jain, Deepti; Visel, Axel; Pennacchio, Len A.; Weiss, Mitchell J.; Blobel, Gerd A.; Hardison, Ross C.

    2015-04-23

    Regulated gene expression controls organismal development, and variation in regulatory patterns has been implicated in complex traits. Thus accurate prediction of enhancers is important for further understanding of these processes. Genome-wide measurement of epigenetic features, such as histone modifications and occupancy by transcription factors, is improving enhancer predictions, but the contribution of these features to prediction accuracy is not known. Given the importance of the hematopoietic transcription factor TAL1 for erythroid gene activation, we predicted candidate enhancers based on genomic occupancy by TAL1 and measured their activity. Contributions of multiple features to enhancer prediction were evaluated based on the results of these and other studies. Results: TAL1-bound DNA segments were active enhancers at a high rate both in transient transfections of cultured cells (39 of 79, or 56%) and transgenic mice (43 of 66, or 65%). The level of binding signal for TAL1 or GATA1 did not help distinguish TAL1-bound DNA segments as active versus inactive enhancers, nor did the density of regulation-related histone modifications. A meta-analysis of results from this and other studies (273 tested predicted enhancers) showed that the presence of TAL1, GATA1, EP300, SMAD1, H3K4 methylation, H3K27ac, and CAGE tags at DNase hypersensitive sites gave the most accurate predictors of enhancer activity, with a success rate over 80% and a median threefold increase in activity. Chromatin accessibility assays and the histone modifications H3K4me1 and H3K27ac were sensitive for finding enhancers, but they have high false positive rates unless transcription factor occupancy is also included. Conclusions: Occupancy by key transcription factors such as TAL1, GATA1, SMAD1, and EP300, along with evidence of transcription, improves the accuracy of enhancer predictions based on epigenetic features.

  14. Best Possible Strategy for Finding Ground States

    SciTech Connect (OSTI)

    Franz, Astrid; Hoffmann, Karl Heinz; Salamon, Peter

    2001-06-04

    Finding the ground state of a system with a complex energy landscape is important for many physical problems including protein folding, spin glasses, chemical clusters, and neural networks. Such problems are usually solved by heuristic search methods whose efficacy is judged by empirical performance on selected examples. We present a proof that, within the large class of algorithms that simulate a random walk on the landscape, threshold accepting is the best possible strategy. In particular, it can perform better than simulated annealing and Tsallis statistics. Our proof is the first example of a provably optimal strategy in this area.

  15. Focus Series: Program Finds Community "Readiness" Is the Key...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Program Finds Community "Readiness" Is the Key to More Retrofits Focus Series: Program Finds Community "Readiness" Is the Key to More Retrofits Focus Series: Program Finds ...

  16. Trigger loop folding determines transcription rate of Escherichia coli’s RNA polymerase

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Mejia, Yara X.; Nudler, Evgeny; Bustamante, Carlos

    2014-12-31

    Two components of the RNA polymerase (RNAP) catalytic center, the bridge helix and the trigger loop (TL), have been linked with changes in elongation rate and pausing. Here, single molecule experiments with the WT and two TL-tip mutants of the Escherichia coli enzyme reveal that tip mutations modulate RNAP’s pause-free velocity, identifying TL conformational changes as one of two rate-determining steps in elongation. Consistent with this observation, we find a direct correlation between helix propensity of the modified amino acid and pause-free velocity. Moreover, nucleotide analogs affect transcription rate, suggesting that their binding energy also influences TL folding. A kineticmore » model in which elongation occurs in two steps, TL folding on nucleoside triphosphate (NTP) binding followed by NTP incorporation/pyrophosphate release, quantitatively accounts for these results. The TL plays no role in pause recovery remaining unfolded during a pause. The model suggests a finely tuned mechanism that balances transcription speed and fidelity.« less

  17. Trigger loop folding determines transcription rate of Escherichia colis RNA polymerase

    SciTech Connect (OSTI)

    Mejia, Yara X.; Nudler, Evgeny; Bustamante, Carlos

    2014-12-31

    Two components of the RNA polymerase (RNAP) catalytic center, the bridge helix and the trigger loop (TL), have been linked with changes in elongation rate and pausing. Here, single molecule experiments with the WT and two TL-tip mutants of the Escherichia coli enzyme reveal that tip mutations modulate RNAPs pause-free velocity, identifying TL conformational changes as one of two rate-determining steps in elongation. Consistent with this observation, we find a direct correlation between helix propensity of the modified amino acid and pause-free velocity. Moreover, nucleotide analogs affect transcription rate, suggesting that their binding energy also influences TL folding. A kinetic model in which elongation occurs in two steps, TL folding on nucleoside triphosphate (NTP) binding followed by NTP incorporation/pyrophosphate release, quantitatively accounts for these results. The TL plays no role in pause recovery remaining unfolded during a pause. The model suggests a finely tuned mechanism that balances transcription speed and fidelity.

  18. UDAC 2014 Findings and Recommendations | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    2014 Findings and Recommendations UDAC 2014 Findings and Recommendations UDAC 2014 Findings and Recommendations (162.67 KB) More Documents & Publications Minutes from December 16, 2013 UDAC Meeting UDAC 2014 Report UDAC Findings and Recommendations 2014

  19. UDAC Findings and Recommendations 2014 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Findings and Recommendations 2014 UDAC Findings and Recommendations 2014 UDAC Findings and Recommendations 2014 (228.2 KB) More Documents & Publications Minutes from December 16, 2013 UDAC Meeting UDAC 2014 Report UDAC 2014 Findings and Recommendations

  20. Landfill aeration worldwide: Concepts, indications and findings

    SciTech Connect (OSTI)

    Ritzkowski, M.; Stegmann, R.

    2012-07-15

    Highlights: Black-Right-Pointing-Pointer Different landfill aeration concepts and accordant application areas are described. Black-Right-Pointing-Pointer Examples of full scale projects are provided for Europe, North-America and Asia. Black-Right-Pointing-Pointer Major project findings are summarised, including prospects and limitations. Black-Right-Pointing-Pointer Inconsistencies between laboratory and full scale results have been elaborated. Black-Right-Pointing-Pointer An explanatory approach in connection with the inconsistencies is provided. - Abstract: The creation of sustainable landfills is a fundamental goal in waste management worldwide. In this connection landfill aeration contributes towards an accelerated, controlled and sustainable conversion of conventional anaerobic landfills into a biological stabilized state associated with a minimised emission potential. The technology has been successfully applied to landfills in Europe, North America and Asia, following different strategies depending on the geographical region, the specific legislation and the available financial resources. Furthermore, methodologies for the incorporation of landfill aeration into the carbon trade mechanisms have been developed in recent years. This manuscript gives an overview on existing concepts for landfill aeration; their application ranges and specifications. For all of the described concepts examples from different countries worldwide are provided, including details regarding their potentials and limitations. Some of the most important findings from these aeration projects are summarised and future research needs have been identified. It becomes apparent that there is a great demand for a systematisation of the available results and implications in order to further develop and optimise this very promising technology. The IWWG (International Waste Working Group) Task Group 'Landfill Aeration' contributes towards the achievement of this goal.

  1. Finding nonoverlapping substructures of a sparse matrix

    SciTech Connect (OSTI)

    Pinar, Ali; Vassilevska, Virginia

    2004-08-09

    Many applications of scientific computing rely on computations on sparse matrices, thus the design of efficient implementations of sparse matrix kernels is crucial for the overall efficiency of these applications. Due to the high compute-to-memory ratio and irregular memory access patterns, the performance of sparse matrix kernels is often far away from the peak performance on a modern processor. Alternative data structures have been proposed, which split the original matrix A into A{sub d} and A{sub s}, so that A{sub d} contains all dense blocks of a specified size in the matrix, and A{sub s} contains the remaining entries. This enables the use of dense matrix kernels on the entries of A{sub d} producing better memory performance. In this work, we study the problem of finding a maximum number of non overlapping rectangular dense blocks in a sparse matrix, which has not been studied in the sparse matrix community. We show that the maximum non overlapping dense blocks problem is NP-complete by using a reduction from the maximum independent set problem on cubic planar graphs. We also propose a 2/3-approximation algorithm for 2 times 2 blocks that runs in linear time in the number of nonzeros in the matrix. We discuss alternatives to rectangular blocks such as diagonal blocks and cross blocks and present complexity analysis and approximation algorithms.

  2. Finding Nonoverlapping Substructures of a Sparse Matrix

    SciTech Connect (OSTI)

    Pinar, Ali; Vassilevska, Virginia

    2005-08-11

    Many applications of scientific computing rely on computations on sparse matrices. The design of efficient implementations of sparse matrix kernels is crucial for the overall efficiency of these applications. Due to the high compute-to-memory ratio and irregular memory access patterns, the performance of sparse matrix kernels is often far away from the peak performance on a modern processor. Alternative data structures have been proposed, which split the original matrix A into A{sub d} and A{sub s}, so that A{sub d} contains all dense blocks of a specified size in the matrix, and A{sub s} contains the remaining entries. This enables the use of dense matrix kernels on the entries of A{sub d} producing better memory performance. In this work, we study the problem of finding a maximum number of nonoverlapping dense blocks in a sparse matrix, which is previously not studied in the sparse matrix community. We show that the maximum nonoverlapping dense blocks problem is NP-complete by using a reduction from the maximum independent set problem on cubic planar graphs. We also propose a 2/3-approximation algorithm that runs in linear time in the number of nonzeros in the matrix. This extended abstract focuses on our results for 2x2 dense blocks. However we show that our results can be generalized to arbitrary sized dense blocks, and many other oriented substructures, which can be exploited to improve the memory performance of sparse matrix operations.

  3. Russian research capabilities: Findings of site visits

    SciTech Connect (OSTI)

    Wester, D.W.

    1994-02-01

    In June 1993, a proposal was presented to the International Environmental Institute (IEI) in Kennewick, Washington, to establish cooperation and coordination to further pursue the interests of the United States of America and the Republic of Russia in the application and promotion of environmental technology; characterization, treatment, handling, isolation, and disposal of hazardous and radioactive materials; conversion of defense sites to other purposes; and technology transfer, cooperative programs, joint technology development and contractual research. In response to this proposal, IEI and Pacific Northwest Laboratory (PNL) jointly provided funding to send Dr. Dennis W. Wester on a fact-finding mission to Novosibirsk, Moscow, and St. Petersburg, Russia. The trip covered a period of eight weeks, six of which were spent in Novosibirsk and adjoining or related cities and one of which was spent in each of Moscow and St. Petersburg. The general objectives of the trip were to establish a basis for cooperation between IEI and the Russian Academy of Sciences (RAS) for future coordination of mutual interests and objectives such as technology acquisition, development, demonstration, application, and commercialization; use of capabilities and assets developed by the U.S. Department of Energy (DOE) and the RAS; and expediting of cooperative agreements, personnel exchanges, joint ventures and other contractual relationships. The particular objectives of this trip were to evaluate the capabilities of the RAS to satisfy the technology needs associated with the cleanup of the Hanford Site and similar sites in the U.S. and to evaluate the expediency of establishing an IEI presence in Russia.

  4. Transcription factors for modification of lignin content in plants

    DOE Patents [OSTI]

    Wang, Huanzhong; Chen, Fang; Dixon, Richard A.

    2015-06-02

    The invention provides methods for modifying lignin, cellulose, xylan, and hemicellulose content in plants, and for achieving ectopic lignification and, for instance, secondary cell wall synthesis in pith cells, by altered regulation of a WRKY transcription factor. Nucleic acid constructs for altered WRKY-TF expression are described. Transgenic plants are provided that comprise modified pith cell walls, and lignin, cellulose, and hemicellulose content. Plants described herein may be used, for example, as improved biofuel feedstock and as highly digestible forage crops.

  5. Text Transcript of the Tribal Energy and Economic Development February

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Webinar: Exploring Your Energy Markets | Department of Energy Development February Webinar: Exploring Your Energy Markets Text Transcript of the Tribal Energy and Economic Development February Webinar: Exploring Your Energy Markets Randy Manion: Good morning or good afternoon wherever you may be and welcome to the second webinar of the 2016 DOE Tribal Renewable Energy Webinar Series: Exploring Your Energy Markets. I'm Randy Manion, today's webinar chair and manager of Western Area Power

  6. Text Transcript of the Tribal Energy and Economic Development March

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Webinar: Transmission and Grid Basics for Tribal Economic and Energy Development | Department of Energy Development March Webinar: Transmission and Grid Basics for Tribal Economic and Energy Development Text Transcript of the Tribal Energy and Economic Development March Webinar: Transmission and Grid Basics for Tribal Economic and Energy Development Sean Esterly: Good morning, or good afternoon, wherever you may be joining us from, and welcome to the third webinar of the 2016 Department of

  7. Transcript of Tribal Energy and Economic Development July Webinar: Project

    Office of Environmental Management (EM)

    Economic Development | Department of Energy June 29 Webinar: Tribal Clean Energy for Sovereignty and Economic Development Transcript of June 29 Webinar: Tribal Clean Energy for Sovereignty and Economic Development Randy Manion: Today's webinar is being recorded and will be made available on DOE's Office of Indian Energy Policy and Programs website along with copies of today's PowerPoint presentations in about one week. Everyone will receive a post-webinar e-mail with the link to the page

  8. Transcript of May 25 Webinar: Tribal Business Structures for Financing

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Projects | Department of Energy 25 Webinar: Tribal Business Structures for Financing Projects Transcript of May 25 Webinar: Tribal Business Structures for Financing Projects Randy Manion: Good morning or good afternoon, wherever you may be, and welcome to the fifth webinar of the 2016 DOE Tribal Renewable Energy webinar series. Today's webinar, "Tribal Business Structure for Financing Projects." I'm Randy Manion, today's webinar chair, and manager of Western Area Power

  9. Meeting of the Electricity Advisory Committee, June 29-30, 2015- Meeting Minutes and Transcripts

    Office of Energy Efficiency and Renewable Energy (EERE)

    Meeting minutes and transcripts for the June 29-30, 2015 meeting of the Electric Advisory Committee.

  10. Meeting of the Electricity Advisory Committee, March 17-18, 2016- Meeting Minutes and Transcripts

    Office of Energy Efficiency and Renewable Energy (EERE)

    Meeting minutes and transcripts for the March 17-18, 2016 meeting of the Electricity Advisory Committee.

  11. Meeting of the Electricity Advisory Committee Meeting, March 26-27, 2015- Meeting Minutes and Transcripts

    Broader source: Energy.gov [DOE]

    Meeting minutes and transcripts for the March 26-27, 2015 meeting of the Electricity Advisory Committee.

  12. Electricity Advisory Committee Meeting, March 12-13, 2014- Meeting Minutes and Transcripts

    Broader source: Energy.gov [DOE]

    Meeting minutes and transcripts for the March 12-13, 2014 meeting of the Electricity Advisory Committee.

  13. Electricity Advisory Committee Meeting, June 11-12, 2012- Meeting Minutes and Transcripts

    Broader source: Energy.gov [DOE]

    Meeting minutes and transcripts for the June 11-12, 2012 meeting of the Electricity Advisory Committee.

  14. Transcript of Tribal Energy and Economic Webinar: Energy Planning for Tribal Economic Development

    Office of Energy Efficiency and Renewable Energy (EERE)

    Transcript for the Tribal Energy and Economic Webinar: Energy Planning for Tribal Economic Development held on Jan. 27, 2016.

  15. Transcript of June 29 Webinar: Tribal Clean Energy for Sovereignty and Economic Development

    Office of Energy Efficiency and Renewable Energy (EERE)

    Transcript for the Office of Indian Energy June 29 webinar, Tribal Clean Energy for Sovereignty and Economic Development.

  16. Meeting of the Electricity Advisory Committee Meeting, September 29-30, 2015- Meeting Minutes and Transcripts

    Broader source: Energy.gov [DOE]

    Meeting minutes and transcripts for the September 29-30, 2015 meeting of the Electric Advisory Committee.

  17. Electricity Advisory Committee Meeting, March 5-6, 2012- Meeting Minutes and Transcripts

    Broader source: Energy.gov [DOE]

    Meeting minutes and transcripts for the March 5-6, 2012 meeting of the Electricity Advisory Committee.

  18. Electricity Advisory Committee Meeting, October 15-16, 2012- Meeting Summaries and Transcripts

    Broader source: Energy.gov [DOE]

    Meeting summaries and transcripts for the October 15-16, 2012 meeting of the Electricity Advisory Committee.

  19. Electricity Advisory Committee Meeting, June 16-17, 2014- Meeting Summaries and Transcripts

    Broader source: Energy.gov [DOE]

    Meeting summaries and transcripts for the June 16-17, 2014 meeting of the Electricity Advisory Committee.

  20. Electricity Advisory Committee Meeting, September 24-25, 2014- Meeting Summaries and Transcripts

    Broader source: Energy.gov [DOE]

    Meeting summaries and transcripts for the September 24-25, 2014 meeting of the Electricity Advisory Committee.

  1. Graduate Transcripts for Current Graduate Institution | U.S. DOE Office of

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Science (SC) Graduate Transcripts for Current Graduate Institution DOE Office of Science Graduate Student Research (SCGSR) Program SCGSR Home Eligibility Benefits Participant Obligations How to Apply Identifying a Collaborating DOE Laboratory Scientist Research Proposal Guidelines Office of Science Priority Research Areas for SCGSR Program Letters of Support Graduate Transcripts for Current Graduate Institution Resources for Handling Transcripts Application Evaluation and Selection

  2. Transcriptional control in Alicyclobacillus acidocaldarius and associated genes, proteins, and methods

    SciTech Connect (OSTI)

    Lee, Brady D.; Thompson, David N.; Apel, William A.; Thompson, Vicki S.; Reed, David W.; Lacey, Jeffrey A.

    2015-11-17

    Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods of modulating transcription or transcription or transcriptional control using isolated and/or purified polypeptides and nucleic acid sequences from Alicyclobacillus acidocaldarius.

  3. Transcriptional control in Alicyclobacillus acidocaldarius and associated genes, proteins, and methods

    DOE Patents [OSTI]

    Lee, Brady Deneys; Thompson, David N; Apel, William A.; Thompson, Vicki Slavchev; Reed, David W; Lacey, Jeffrey A

    2014-05-06

    Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods of modulating transcription or transcription or transcriptional control using isolated and/or purified polypeptides and nucleic acid sequences from Alicyclobacillus acidocaldarius.

  4. The transcriptional diversity of 25 Drosophila cell lines

    SciTech Connect (OSTI)

    Cherbas, Lucy; Willingham, Aarron; Zhang, Dayu; Yang, Li; Zou, Yi; Eads, Brian D.; Carlson, Joseph W.; Landolin, Jane M.; Kapranov, Philipp; Dumais, Jacqueline; Samsonova, Anastasia; Choi, Jeong-Hyeon; Roberts, Johnny; Davis, Carrie A.; Tang, Haixu; van Baren, Marijke J.; Ghosh, Srinka; Dobin, Alexander; Bell, Kim; Lin, Wei; Langton, Laura; Duff, Michael O.; Tenney, Aaron E.; Zaleski, Chris; Brent, Michael R.; Hoskins, Roger A.; Kaufman, Thomas C.; Andrews, Justen; Graveley, Brenton R.; Perrimon, Norbert; Celniker, Susan E.; Gingeras, Thomas R.; Cherbas, Peter

    2010-11-15

    Drosophila melanogaster cell lines are important resources for cell biologists. Here, we catalog the expression of exons, genes, and unannotated transcriptional signals for 25 lines. Unannotated transcription is substantial (typically 19% of euchromatic signal). Conservatively, we identify 1405 novel transcribed regions; 684 of these appear to be new exons of neighboring, often distant, genes. Sixty-four percent of genes are expressed detectably in at least one line, but only 21% are detected in all lines. Each cell line expresses, on average, 5885 genes, including a common set of 3109. Expression levels vary over several orders of magnitude. Major signaling pathways are well represented: most differentiation pathways are off and survival/growth pathways on. Roughly 50% of the genes expressed by each line are not part of the common set, and these show considerable individuality. Thirty-one percent are expressed at a higher level in at least one cell line than in any single developmental stage, suggesting that each line is enriched for genes characteristic of small sets of cells. Most remarkable is that imaginal discderived lines can generally be assigned, on the basis of expression, to small territories within developing discs. These mappings reveal unexpected stability of even fine-grained spatial determination. No two cell lines show identical transcription factor expression. We conclude that each line has retained features of an individual founder cell superimposed on a common cell line gene expression pattern. Wereport the transcriptional profiles of 25 Drosophila melanogaster cell lines, principally by whole-genome tiling microarray analysis of total RNA, carried out as part of the modENCODE project. The data produced in this study add to our knowledge of the cell lines and of the Drosophila transcriptome in several ways. We summarize the expression of previously annotated genes in each of the 25 lines with emphasis on what those patterns reveal

  5. EA-1922: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1922: Finding of No Significant Impact Combined Power and Biomass Heating System, Fort Yukon, Alaska Based on the EA, DOE finds that providng ...

  6. EIS-0372: Record of Decision and Floodplain Statement of Findings...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Record of Decision and Floodplain Statement of Findings EIS-0372: Record of Decision and Floodplain Statement of Findings Bangor Hydro-Electric Company Northeast Reliability...

  7. EIS-0456: Record of Decision and Floodplain Statement of Findings...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Record of Decision and Floodplain Statement of Findings EIS-0456: Record of Decision and Floodplain Statement of Findings Cushman Hydroelectric Project, Mason County, Washington...

  8. EA-1378: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    378: Finding of No Significant Impact EA-1378: Finding of No Significant Impact Site-Wide Environmental Assessment of National Renewable Energy Laboratory's National Wind ...

  9. EA-1698: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    8: Finding of No Significant Impact EA-1698: Finding of No Significant Impact Baldwin Wind Energy Center Project Baldwin Wind, LLC has requested to interconnect their proposed new...

  10. EA-1807: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1807: Finding of No Significant Impact Heartland Community College Wind Energy Project, Normal, McLean County, Illinois The Department of...

  11. EA-1819: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1819: Finding of No Significant Impact Kilowatts for Kenston Wind Energy Project, Chagrin Falls, Geauga County, Ohio The Department of Energy's...

  12. EA-1938: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1938: Finding of No Significant Impact Grieve Unit CO2 Enhanced Recovery Project, Natrona County, WY Western has reviewed BLM's EA "Grieve...

  13. EMGeo: Risk Minimizing Software for Finding Offshore Fossil Fuels...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Find More Like This Return to Search EMGeo: Risk Minimizing Software for Finding Offshore ... developed advanced software for discovering and mapping offshore fossil fuel deposits. ...

  14. EA-1605: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    Finding of No Significant Impact EA-1605: Finding of No Significant Impact Biomass Cogeneration and Heating Facilities at the Savannah River Site, South Carolina The Department of...

  15. EA-1784: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    4: Finding of No Significant Impact EA-1784: Finding of No Significant Impact Fotowatio Nevada Solar, LLC's APEX Solar Power Project in Clark County, Nevada The Department of...

  16. EA-1862: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1862: Finding of No Significant Impact Oneida Seven Generations Corporation: Energy Recovery Project, Green Bay, Wisconsin The U.S. Department...

  17. EA-1923: Mitigated Finding of No Significant Impact | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Mitigated Finding of No Significant Impact EA-1923: Mitigated Finding of No Significant Impact Green Energy School Wind Turbine Project on Saipan, Commonwealth of the Northern ...

  18. Jefferson Lab finds its man Mont (Inside Business) | Jefferson...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    https:www.jlab.orgnewsarticlesjefferson-lab-finds-its-man-mont-inside-business Jefferson Lab finds its man Mont Hugh Montgomery Hugh Montgomery, a British nuclear physicist...

  19. EA-1802: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    2: Finding of No Significant Impact EA-1802: Finding of No Significant Impact Chicago View Wind Project Chicago Heights, Cook County, Illinois The Department of Energy's Proposed...

  20. Community Wind Handbook/Find an Installer | Open Energy Information

    Open Energy Info (EERE)

    * Submit Permit Applications * Find an Installer * Purchase Equipment * Plan for Maintenance Find an Installer Homeowners, ranchers, and small businesses can install wind...

  1. EA-1947: Draft Revised Finding of No Significant Impact | Department...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    1947: Draft Revised Finding of No Significant Impact EA-1947: Draft Revised Finding of No Significant Impact Transfer of the Kansas City Plant, Kansas City, Missouri The National ...

  2. EA-1733: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1733: Finding of No Significant Impact Calpine Enhanced Geothermal Systems Project, Sonoma County, California DOE determined that providing...

  3. EA-1839: Final Environmental Assessment and Finding of No Significant...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    39: Final Environmental Assessment and Finding of No Significant Impact EA-1839: Final Environmental Assessment and Finding of No Significant Impact Department of Energy Loan ...

  4. EA-1829: Final Environmental Assessment and Finding of No Significant...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Final Environmental Assessment and Finding of No Significant Impact EA-1829: Final Environmental Assessment and Finding of No Significant Impact Phycal Algae Pilot Project, Wahiawa...

  5. EA-1866: Final Environmental Assessment and Finding of No Significant...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    6: Final Environmental Assessment and Finding of No Significant Impact EA-1866: Final Environmental Assessment and Finding of No Significant Impact Argonne National Laboratory...

  6. EA-1889: Final Environmental Assessment and Finding of No Significant...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Final Environmental Assessment and Finding of No Significant Impact EA-1889: Final Environmental Assessment and Finding of No Significant Impact Disposal of Decommissioned,...

  7. EA-1869: Final Environmental Assessment and Finding of No Significant...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    69: Final Environmental Assessment and Finding of No Significant Impact EA-1869: Final Environmental Assessment and Finding of No Significant Impact General Motors LLC, Electric...

  8. EA-1769: Final Environmental Assessment and Finding of No Significant...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    9: Final Environmental Assessment and Finding of No Significant Impact EA-1769: Final Environmental Assessment and Finding of No Significant Impact Houston Advanced Research...

  9. Innovative Financing Solutions: Finding Money for Your Energy...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Innovative Financing Solutions: Finding Money for Your Energy Efficiency Projects Innovative Financing Solutions: Finding Money for Your Energy Efficiency Projects Provides an ...

  10. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact Silver Peak Area Geothermal Exploration Project, Esmeralda County, NV December 7, 2012 EA-1863: Finding of No Significant Impact Vegetation...

  11. EA-1984: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1984: Finding of No Significant Impact Disposition of Five Signature Properties at Idaho National Laboratory DOE has decided to implement the...

  12. EA-1832: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    32: Finding of No Significant Impact EA-1832: Finding of No Significant Impact Rainer Biogas LLC Community Anaerobic Manure Digester, Enumclaw, King County, Washington The U.S....

  13. EA-1785: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    5: Finding of No Significant Impact EA-1785: Finding of No Significant Impact Phase III Anthropogenic Test Characterization Project, Mobile County, Alabama Based on the analyses in...

  14. EA-1853: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of No Significant Impact EA-1853: Finding of No Significant Impact Perrin Ranch Wind Energy Interconnection Project, Coconino County, Arizona Based on findings and analysis in...

  15. Untitled Document

    U.S. Energy Information Administration (EIA) Indexed Site

    3 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration This is an unedited Transcript of the Fall Meeting of the Energy Information Administration and the American Statistical Association Committee on Energy Statistics. The meeting took place October 16 and 17, 2003 in the Forrestal Building at 1000 Independence Ave., S.W., Washington, D.C. 20585. Most of the sessions were in 8E-089, although several break out sessions were in

  16. Untitled Document

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Revision to the Analysis of the Impacts of the Clean Power Plan May 27, 2015 Minor changes to Table 7. Nuclear power projections in three cases

    3 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration This is an unedited Transcript of the Fall Meeting of the Energy Information Administration and the American Statistical Association Committee on Energy Statistics. The meeting took place October 16 and 17, 2003 in the Forrestal

  17. Updated Buildings Sector Appliance and Equipment Costs and Efficiency

    Gasoline and Diesel Fuel Update (EIA)

    3 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration This is an unedited Transcript of the Fall Meeting of the Energy Information Administration and the American Statistical Association Committee on Energy Statistics. The meeting took place October 16 and 17, 2003 in the Forrestal Building at 1000 Independence Ave., S.W., Washington, D.C. 20585. Most of the sessions were in 8E-089, although several break out sessions were in

  18. Determining physical constraints in transcriptional initiationcomplexes using DNA sequence analysis

    SciTech Connect (OSTI)

    Shultzaberger, Ryan K.; Chiang, Derek Y.; Moses, Alan M.; Eisen,Michael B.

    2007-07-01

    Eukaryotic gene expression is often under the control ofcooperatively acting transcription factors whose binding is limited bystructural constraints. By determining these structural constraints, wecan understand the "rules" that define functional cooperativity.Conversely, by understanding the rules of binding, we can inferstructural characteristics. We have developed an information theory basedmethod for approximating the physical limitations of cooperativeinteractions by comparing sequence analysis to microarray expressiondata. When applied to the coordinated binding of the sulfur amino acidregulatory protein Met4 by Cbf1 and Met31, we were able to create acombinatorial model that can correctly identify Met4 regulatedgenes.

  19. Microsoft Word - Chicago Transcript 10 07 08 FINAL.doc

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    17/2008 Chicago, Illinois Page 1 U.S. Department of Energy Pre-Congestion Study Regional Workshops for the 2009 National Electric Congestion Study Chicago, IL September 17, 2008 8:30 a.m.-12:00 p.m. Transcript David Meyer: Well, good morning, ladies and gentlemen. I'm David Meyer from the Department of Energy. And I welcome you to our workshop on our 2009 congestion study. Before I get started in a brief presentation, I want to introduce some of my colleagues here. Lot Cooke, who's not at this

  20. Microsoft Word - Consolidated Final Transcript of CBS Tempe AZ

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    9 CONSENT-BASED SITING PUBLIC MEETING Phoenix Marriott Tempe at the Buttes 2000 W Westcourt Way Tempe, AZ 85282 June 23, 2016 FULL TRANSCRIPT Mr. Jim Hamilton. Good afternoon. And for those joining in a later time zone, good evening. Welcome to Tempe and to the sixth in a series of public meetings the Department is hosting on its Consent-Based Siting Program. Thank you all for being here today. My name is Jim Hamilton - I'm an advisor to the Department as part of their Consent-Based Siting Team

  1. Microsoft Word - Consolidated Meeting Denver Verbatim Transcript_Final

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Embassy Suites Denver - Stapleton 4444 Havana Street Denver, CO 80239 May 24, 2016 FULL TRANSCRIPT Mr. Jim Hamilton. Good afternoon. And for those in a later time zone via webinar, good evening. Welcome to Denver and to the fourth in a series of public meetings the Department is hosting on its Consent-Based Siting Program. Move in if you want - you don't have to sit in the periphery if you don't want to. We want to try to make it a little more intimate. Thank you for being here today. My name is

  2. Floodplain Assessment and Statement of Findings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    and Statement of Findings Floodplain Assessment and Statement of Findings Floodplain Assessment and Statement of Findings for Installation of a Renewable Energy Anaerobic Digester Facility at the University of California, Davis in Yolo County, California. Statement of Findings (450.47 KB) More Documents & Publications Floodplain Assessment for Installation of a Renewable Energy Anaerobic Digester Facility

  3. Comparative genomics of transcriptional regulation of methionine metabolism in proteobacteria

    SciTech Connect (OSTI)

    Leyn, Semen A.; Suvorova, Inna A.; Kholina, Tatiana D.; Sherstneva, Sofia S.; Novichkov, Pavel S.; Gelfand, Mikhail S.; Rodionov, Dmitry A.; Kuipers, Oscar P.

    2014-11-20

    Methionine metabolism and uptake genes in Proteobacteria are controlled by a variety of RNA and DNA regulatory systems. We have applied comparative genomics to reconstruct regulons for three known transcription factors, MetJ, MetR, and SahR, and three known riboswitch motifs, SAH, SAM-SAH, and SAM_alpha, in ~200 genomes from 22 taxonomic groups of Proteobacteria. We also identified two novel regulons: a SahR-like transcription factor SamR controlling various methionine biosynthesis genes in the Xanthomonadales group, and a potential RNA regulatory element with terminator-antiterminator mechanism controlling the metX or metZ genes in beta-proteobacteria. For each analyzed regulator we identified the core, taxon-specific and genome-specific regulon members. By analyzing the distribution of these regulators in bacterial genomes and by comparing their regulon contents we elucidated possible evolutionary scenarios for the regulation of the methionine metabolism genes in Proteobacteria.

  4. Comparative genomics of transcriptional regulation of methionine metabolism in proteobacteria

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Leyn, Semen A.; Suvorova, Inna A.; Kholina, Tatiana D.; Sherstneva, Sofia S.; Novichkov, Pavel S.; Gelfand, Mikhail S.; Rodionov, Dmitry A.; Kuipers, Oscar P.

    2014-11-20

    Methionine metabolism and uptake genes in Proteobacteria are controlled by a variety of RNA and DNA regulatory systems. We have applied comparative genomics to reconstruct regulons for three known transcription factors, MetJ, MetR, and SahR, and three known riboswitch motifs, SAH, SAM-SAH, and SAM_alpha, in ~200 genomes from 22 taxonomic groups of Proteobacteria. We also identified two novel regulons: a SahR-like transcription factor SamR controlling various methionine biosynthesis genes in the Xanthomonadales group, and a potential RNA regulatory element with terminator-antiterminator mechanism controlling the metX or metZ genes in beta-proteobacteria. For each analyzed regulator we identified the core, taxon-specific andmore » genome-specific regulon members. By analyzing the distribution of these regulators in bacterial genomes and by comparing their regulon contents we elucidated possible evolutionary scenarios for the regulation of the methionine metabolism genes in Proteobacteria.« less

  5. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    in Kahuku, O'ahu, Hawaii May 18, 2010 EA-1699: Finding of No Significant Impact PopeDouglas Third Combustor Expansion Project, Alexandria, Minnesota May 7, 2010 EA-1635: Finding...

  6. EIS-0365: Record of Decision and Floodplain Statement of Findings...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Record of Decision and Floodplain Statement of Findings EIS-0365: Record of Decision and Floodplain Statement of Findings Imperial-Mexicali 230-kV Transmission Lines DOE announces...

  7. Accident Investigation Board (AIB) findings about the drum breach...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Accident Investigation Board findings Accident Investigation Board (AIB) findings about the drum breach at WIPP WHEN: Apr 23, 2015 5:30 PM - 7:00 PM WHERE: Fuller Lodge 2132 ...

  8. EA-1767: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    7: Finding of No Significant Impact EA-1767: Finding of No Significant Impact Virginia State Energy Program's Cephas C&D Wastes Biomass Project, Richmond, Virginia Based on the...

  9. EA-1814: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1814: Finding of No Significant Impact City of Montpelier Combined Heat and Power, and District Energy System, Montpelier, Vermont The U.S....

  10. EA-1726: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1726: Finding of No Significant Impact Kahuku Wind Power, LLC, Construction of the Kahuku Wind Power Facility in Kahuku, O'ahu, Hawaii The ...

  11. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Project, Zachary, Louisiana November 24, 2010 EA-1797: Finding of No Significant Impact Agua Caliente Solar Project in Yuma County, Arizona November 19, 2010 EA-1727: Finding of No...

  12. Got a Minute? Which Higgs did we find?

    ScienceCinema (OSTI)

    Stupak, John

    2014-09-15

    Dr. John Stupak talks about the discovery of the Higgs boson. Did scientists find the Higgs boson predicted back in 1964 or did they find just one of a group of particles, with the others still to be found?

  13. Commercial Building Energy Asset Score: Pilot Findings and Program...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Commercial Building Energy Asset Score: Pilot Findings and Program Update The webinar was held on April 16, 2014, to share the findings from the 2013 Pilot and provide a program ...

  14. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    2012 EA-1812: Finding of No Significant Impact Haxtun Wind Energy Project, Logan and Phillips Counties, CO January 3, 2012 EA-1606: Finding of No Significant Impact Proposed Use...

  15. EERE 2014 Wind Technologies Market Report Finds Wind Power at...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    2014 Wind Technologies Market Report Finds Wind Power at Record Low Prices EERE 2014 Wind Technologies Market Report Finds Wind Power at Record Low Prices August 10, 2015 - 11:00am ...

  16. EA-1894: Finding of No Significant Impact | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    Albeni Falls Flexible Winter Lake Operations, Bonner, Idaho As a result of the analysis in this EA, I have the following findings. I find that: 1) the FWPO is not a substantial...

  17. EA-1904: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    EA-1904: Finding of No Significant Impact Linac Coherent Light Source II at Stanford Linear Accelerator Laboratory, San Mateo, CA Based on the findings in the EA, DOE has...

  18. Title 16 USC 1600 Congressional Findings | Open Energy Information

    Open Energy Info (EERE)

    600 Congressional Findings Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- StatuteStatute: Title 16 USC 1600 Congressional FindingsLegal Abstract...

  19. EA-1947: Finding of No Significant Impact | Department of Energy

    Energy Savers [EERE]

    7: Finding of No Significant Impact EA-1947: Finding of No Significant Impact Transfer of ... and NNSA is issuing this FONSI. EA-1947FONSI-2013.pdf (169.65 KB) More Documents ...

  20. EA-1833: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1833: Finding of No Significant Impact The City of El Dorado Wind Energy Project, El Dorado, Butler County, Kansas The U.S. Department of...

  1. EA-1928: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1928: Finding of No Significant Impact White-Tailed Deer Management at Brookhaven National Lab, Upton, New York Based on the information in the...

  2. EA-1665: Final Environmental Assessment and Finding of No Significant...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    5: Final Environmental Assessment and Finding of No Significant Impact EA-1665: Final Environmental Assessment and Finding of No Significant Impact Davis-Kingman Tap 69-kV...

  3. Rainbows and Leprechauns: Finding Gold in Partnerships (101)...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Rainbows and Leprechauns: Finding Gold in Partnerships (101) Rainbows and Leprechauns: Finding Gold in Partnerships (101) March 17, 2016 1:00PM to 2:30PM EDT

  4. STEM Volunteer Training: Types of Volunteering and Where to Find...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Types of Volunteering and Where to Find Opportunities STEM Volunteer Training: Types of Volunteering and Where to Find Opportunities April 14, 2016 3:00PM to 4:00PM EDT

  5. Got a Minute? Which Higgs did we find?

    SciTech Connect (OSTI)

    Stupak, John

    2014-06-26

    Dr. John Stupak talks about the discovery of the Higgs boson. Did scientists find the Higgs boson predicted back in 1964 or did they find just one of a group of particles, with the others still to be found?

  6. EA-1809: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1809: Finding of No Significant Impact White Earth Nation Wind Energy Project II, Becker and Mahnomen Counties, MN DOE's Golden Field Office has...

  7. EA-1933: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1933: Finding of No Significant Impact Yakama Nation Drop 4 Hydropower Project, Yakama Nation Reservation, WA Based on the Final EA, DOE has...

  8. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    County, Nevada June 9, 2011 EA-1770: Finding of No Significant Impact Photovoltaic Solar Project at the Durango, Colorado Site June 3, 2011 EA-1858: Finding of No Significant...

  9. ULTRA-DEEP WATER ADVISORY COMMITTEE FINDINGS AND

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ULTRA-DEEP WATER ADVISORY COMMITTEE FINDINGS AND RECOMMENDATIONS 2014 ULTRA-DEEPWATER ADVISORY COMMITTEE COMMITTEE FINDINGS AND RECOMMENDATIONS i Table of Contents Research and Development Program Committee ............................................ 1 Sunset Committee .................................................................................................. 5 Advisory Committee Members ........................................................................... 7 ULTRA-DEEPWATER

  10. EA-1917: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1917: Finding of No Significant Impact Wave Energy Test Facility Project, Newport, OR Based on the information presented in the Final EA, (DOE...

  11. EA-1656: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1656: Finding of No Significant Impact MARET Center at Crowder College, Neosho, Missouri The U.S. Department of Energy is preparing an...

  12. EIS-0369: Floodplain Statement of Finding | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    9: Floodplain Statement of Finding EIS-0369: Floodplain Statement of Finding Rail Alignment for the Construction and Operation of a Railroad in Nevada to a Geologic Repository at Yucca Mountain, Nye County, Nevada Floodplain Statement of Findings for the Final Environmental Impact Statement for a Rail Alignment for the Construction and Operation of a Railroad in Nevada to a Geologic Repository at Yucca Mountain, Nye County, Nevada Floodplain Statement of Findings for the Final Environmental

  13. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Findings of No Significant Impact (FONSI) Findings of No Significant Impact (FONSI) Findings of No Significant Impact are public documents issued by a Federal agency briefly presenting the reasons why an action for which the agency has prepared an environmental assessment will not have a significant effect on the human environment and, therefore, will not require preparation of an environmental impact statement. If you have any trouble finding a specific document, please contact

  14. Finding and Winning Simplified Acquisitions, Part 2 | Department of Energy

    Energy Savers [EERE]

    Finding and Winning Simplified Acquisitions, Part 2 Finding and Winning Simplified Acquisitions, Part 2 Presented at the 15th Annual DOE Small Business Forum & Expo by: Guy Timberlake, Chief Visionary Officer and Chief Executive Officer, The American Small Business Coalition Finding and Winning Simplified Acquisitions, Part 2 (6.47 MB) More Documents & Publications Finding and Winning Simplified Acquisitions, Part 1 December 12, 2013 Business Opportunity Session Presentations Acquisition

  15. Portsmouth Site Treatment Plan Director's Final Findings and Orders |

    Energy Savers [EERE]

    Department of Energy Treatment Plan Director's Final Findings and Orders Portsmouth Site Treatment Plan Director's Final Findings and Orders Portsmouth Site Treatment Plan Director's Final Findings and Orders is an agreement between the Ohio EPA and DOE approving the Site Treatment Plan and setting forth guidelines for storage and treatment of mixed wastes at the Portsmouth Facility. Portsmouth Site Treatment Plan Director's Final Findings and Order - 1995 (3.65 MB) More Documents &

  16. Transcript of April 3, 2013, TAP webinar titled State Energy Strategic Planning

    Broader source: Energy.gov [DOE]

    U.S. Department of Energy (DOE) Technical Assistance Program (TAP) transcript of a TAP webinar held on April 3, 2013 and dealing with state energy strategic planning.

  17. A new chapter in the transcription SAGA (Journal Article) | SciTech...

    Office of Scientific and Technical Information (OSTI)

    SciTech Connect Search Results Journal Article: A new chapter in the transcription SAGA ... Publication Date: 2012-11-05 OSTI Identifier: 1047303 Resource Type: Journal Article ...

  18. Successful Partnerships and Lessons from the Field, Part 1, Webinar Transcript

    Broader source: Energy.gov [DOE]

    Part 1-Successful Partnerships and Lessons from the Field Webinar Transcript, from the U.S. Department of Energy Technical Assistance Program (TAP).

  19. Better Buildings Webinar Transcription- Financial Vehicles within an Integrated Energy Efficiency Program

    Broader source: Energy.gov [DOE]

    "Financial Vehicles Within an Integrated Energy Efficiency Program," webinar transcript from the U.S. Department of Energy's Better Buildings program.

  20. Electricity Advisory Committee Meeting, June 5-6, 2013- Meeting Summaries and Transcripts

    Broader source: Energy.gov [DOE]

    Meeting summaries and transcripts for the June 5-6, 2013 meeting of the Electricity Advisory Committee.

  1. Transcript of Office of Indian Energy Webinar: Inter-Tribal Technical Assistance Energy Providers Network FOA

    Broader source: Energy.gov [DOE]

    Transcript of the DOE Office of Indian Energy webinar about the Inter-Tribal Technical Assistance Energy Providers Network funding opportunity.

  2. Public Meeting Transcript: Sustainable Design Standards for Federal...

    Office of Environmental Management (EM)

    ... building products such as window 14 frames, roofing ... or residential building, you find 8 it on the roof, in the power conduits and cable 9 coverings which speak to ...

  3. Pivotal roles of p53 transcription-dependent and -independent pathways in manganese-induced mitochondrial dysfunction and neuronal apoptosis

    SciTech Connect (OSTI)

    Wan, Chunhua; Ma, Xa; Shi, Shangshi; Zhao, Jianya; Nie, Xiaoke; Han, Jingling; Xiao, Jing; Wang, Xiaoke; Jiang, Shengyang; Jiang, Junkang

    2014-12-15

    Chronic exposure to excessive manganese (Mn) has been known to lead to neuronal loss and a clinical syndrome resembling idiopathic Parkinson's disease (IPD). p53 plays an integral role in the development of various human diseases, including neurodegenerative disorders. However, the role of p53 in Mn-induced neuronal apoptosis and neurological deficits remains obscure. In the present study, we showed that p53 was critically involved in Mn-induced neuronal apoptosis in rat striatum through both transcription-dependent and -independent mechanisms. Western blot and immunohistochemistrical analyses revealed that p53 was remarkably upregulated in the striatum of rats following Mn exposure. Coincidentally, increased level of cleaved PARP, a hallmark of apoptosis, was observed. Furthermore, using nerve growth factor (NGF)-differentiated PC12 cells as a neuronal cell model, we showed that Mn exposure decreased cell viability and induced apparent apoptosis. Importantly, p53 was progressively upregulated, and accumulated in both the nucleus and the cytoplasm. The cytoplasmic p53 had a remarkable distribution in mitochondria, suggesting an involvement of p53 mitochondrial translocation in Mn-induced neuronal apoptosis. In addition, Mn-induced impairment of mitochondrial membrane potential (ΔΨm) could be partially rescued by pretreatment with inhibitors of p53 transcriptional activity and p53 mitochondrial translocation, Pifithrin-α (PFT-α) and Pifithrin-μ (PFT-μ), respectively. Moreover, blockage of p53 activities with PFT-α and PFT-μ significantly attenuated Mn-induced reactive oxidative stress (ROS) generation and mitochondrial H{sub 2}O{sub 2} production. Finally, we observed that pretreatment with PFT-α and PFT-μ ameliorated Mn-induced apoptosis in PC12 cells. Collectively, these findings implicate that p53 transcription-dependent and -independent pathways may play crucial roles in the regulation of Mn-induced neuronal death. - Highlights: • p53 is robustly

  4. Alternative Fuels Data Center: Minnesota School District Finds Cost

    Alternative Fuels and Advanced Vehicles Data Center [Office of Energy Efficiency and Renewable Energy (EERE)]

    Savings, Cold-Weather Reliability with Propane Buses Minnesota School District Finds Cost Savings, Cold-Weather Reliability with Propane Buses to someone by E-mail Share Alternative Fuels Data Center: Minnesota School District Finds Cost Savings, Cold-Weather Reliability with Propane Buses on Facebook Tweet about Alternative Fuels Data Center: Minnesota School District Finds Cost Savings, Cold-Weather Reliability with Propane Buses on Twitter Bookmark Alternative Fuels Data Center: Minnesota

  5. Commercial Building Energy Asset Score: Pilot Findings and Program Update |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Score: Pilot Findings and Program Update Commercial Building Energy Asset Score: Pilot Findings and Program Update The webinar was held on April 16, 2014, to share the findings from the 2013 Pilot and provide a program update on the Commercial Building Energy Asset Score. Presentation (1.74 MB) More Documents & Publications Commercial Building Energy Asset Rating HES Program Update and Scoring Tool v2014 Release Commercial Building Energy Asset Score: 2013 Pilot

  6. EA-2010: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-2010: Finding of No Significant Impact Alternating Gradient Synchrotron Complex Improvements at Brookhaven National Laboratory; Upton, New York DOE issued a Finding of No Significant Impact for the proposed activities at the Brookhaven National Laboratory Alternating Gradient Synchrotron Complex, including upgrading the Accelerator Test Facility to 10 GeV by adding laser acceleration, upgrading the Linac proton beam current, upgrading the facilities to produce

  7. EA-2022: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-2022: Finding of No Significant Impact Western Area Power Administration adopted and approved a final EA, prepared by the Bureau of Reclamation with Western as a cooperating agency, that assesses the potential environmental impacts of a proposal to develop a 9.4 megawatt hydroelectric project at the existing Helena Valley Pumping Plant site at Canyon Ferry Dam on the Missouri River near Helena, Montana. Western issued a finding of no significant impact for the

  8. How to Find Ames Laboratory | The Ames Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    How to Find Ames Laboratory Finding the Ames Laboratory The Ames Laboratory has it's main administrative offices in the Technical and Administrative Services Facility (TASF) building on the Iowa State University Campus. Technical and Administrative Services Facility (TASF) 2408 Pammel Drive, Ames, IA 50011-1015 For directions from the Des Moines Airport please use the link with the following directions: https://goo.gl/maps/c6SOK Finding the Ames Laboratory on the ISU Campus Image Click on the

  9. FOIA-Summary of Findings: Circumstances surrounding decisions regarding the

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    security clearance, access and work assignments | Department of Energy FOIA-Summary of Findings: Circumstances surrounding decisions regarding the security clearance, access and work assignments FOIA-Summary of Findings: Circumstances surrounding decisions regarding the security clearance, access and work assignments FOIA request FOIA-Summary of Findings: Circumstances surrounding decisions regarding the security clearance, access and work assignments (4.61 MB) More Documents &

  10. EA-1440: Finding of No Signficant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    : Finding of No Signficant Impact EA-1440: Finding of No Signficant Impact National Renewable Energy Laboratory's South Table Mountain Complex The Proposed Action is to operate the STM complex for alternative energy research with new and improved capabilities to support DOE"s mission to research, develop and transfer to industry renewable energy technologies. Finding of No Signficant Impact for the Site-Wide Environmental Assessment of the National Renewable Energy Laboratory's South Table

  11. EA-1406: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    06: Finding of No Significant Impact EA-1406: Finding of No Significant Impact Ground Water Compliance at the New Rifle, Colorado, UMTRA Project Site On the basis of the Environmental Assessment. which analyzed relevant environmental issues and addressed the concerns of stakeholders, DOE finds that no significant impact will result from implementing the planned action at the New Rifle site. This FONSI is documented pursuant to NEPA, the Council on Environmental Quality's "Regulations for

  12. Find Funding | U.S. DOE Office of Science (SC)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Find Funding Funding Opportunities Funding Opportunities Home Grants & Contracts Support Award Search / Public Abstracts Find Funding Early Career Research Program Statement on Digital Data Management Acknowledgements of Federal Support Contact Information Office of Science U.S. Department of Energy 1000 Independence Ave., SW Washington, DC 20585 P: (202) 586-5430 Find Funding Print Text Size: A A A FeedbackShare Page Additional information on funding opportunities, including program

  13. Portsmouth Integration Director's Final Findings and Order | Department of

    Energy Savers [EERE]

    Energy Integration Director's Final Findings and Order Portsmouth Integration Director's Final Findings and Order Portsmouth Integration Director's Final Findings and Order purpose is to: integrate the on-site work required for specific units to avoid duplication of effort, and efficiently perform sitewide ground water monitoring and surveillance and maintenance activities; recognize that a substantial portion of the tasks required under existing approved closure plans for certain units have

  14. EA-1495: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    5: Finding of No Significant Impact EA-1495: Finding of No Significant Impact USEC Inc. American Centrifuge Lead Cascade Facility at Piketon, Ohio The purpose of NRC's proposed action is to authorize possession and use of source and special nuclear material at the Lead Cascade in Piketon, Ohio. Finding of No Significant Impact for the United States Enrichment Corporation Incorporated, American Centrifuge Lead Cascade Facility at Piketon, Ohio (369.61 KB) More Documents & Publications

  15. EA-1193: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    EA-1193: Finding of No Significant Impact Atmospheric Radiation Measurement (ARM) Program North Slope of Alaska and Adjacent Arctic Ocean Cloud and Radiation Testbed (CART) Site The ...

  16. PPPL physicists find clue to formation of magnetic fields around...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    results, in the range in which they are done, strongly suggest that such dynamo action is possible." These findings might lead to greater understanding of the behavior of...

  17. EA-1685: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of No Significant Impact EA-1685: Finding of No Significant Impact Experimental Coal Flow Program at Clemson University The Western Area Power Administration (Western) is the...

  18. CLASS DEVIATION FINDINGS AND DETERMINATION TO IMPLEMENT AN APPROPRIATI...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    PROVISION RELATED TO INTERNAL CONFIDENTIALITY AGREEMENTS AND THE REPORTING OF FRAUD, WASTE, AND ABUSE Findings I. Section 743 of Division E, Title VII of the Consolidated...

  19. Finding of No Significant Impact, Federal Register, 73 FR 2017...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    73 FR 2017, January 11, 2008 Document details the finding of no significant impact of the Energy-Efficient Performance Requirements for New Federal Commercial and Residential...

  20. Main Page Main Page: Find energy information and data. Share...

    Open Energy Info (EERE)

    Main Page Main Page: Find energy information and data. Share knowledge. Connect with people. energy datasets energy community Main Page energy information open data 0...

  1. EA-1864: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Impact EA-1864: Finding of No Significant Impact Electrical District 5 - Palo Verde Hub Project The U.S. Department of Energy (DOE) Western Area Power Administration...

  2. EA-2024: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of Energy, National Nuclear Security Administration (NNSA), issued this finding of no ... involved with the transportation of the plutonium are maintained within acceptable limits. ...

  3. Floodplain and Wetland Assessment and Statement of Findings

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    and Statement of Findings for Construction of a Second Full Service Access Road ... with the construction of a second full service access road to the NREL STM Site. ...

  4. EA-1719: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Impact EA-1719: Finding of No Significant Impact Novolyte Technologies, Inc. Electric Drive Vehicle Battery and Component Manufacturing Initiative Project, Zachary, Louisiana DOE...

  5. EA-0921: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    EA-0921: Finding of No Significant Impact Ambulatory Research and Education Center, Oregon Health ... the meaning of NEPA. Therefore, an environmental impact statement is not required. ...

  6. Energy Department Report Finds Major Potential to Increase Clean...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Department Report Finds Major Potential to Increase Clean Hydroelectric ... to develop electric power generation at existing dams across the United States that aren't ...

  7. Oregon 401 Evaluation and Findings Checklist | Open Energy Information

    Open Energy Info (EERE)

    401 Evaluation and Findings Checklist Jump to: navigation, search OpenEI Reference LibraryAdd to library PermittingRegulatory Guidance - Checklist: Oregon 401 Evaluation and...

  8. EA-1797: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    EA-1797: Finding of No Significant Impact Agua Caliente Solar Project in Yuma County, ... transmission line proposed by Agua Caliente Solar, LLC in Yuma County, Arizona. ...

  9. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Uranium July 1, 2009 EA-1645: Finding of No Significant Impact Loan Guarantee for Sage Electrochromics SageGlass High Volume Manufacturing Facility in Faribault, MN June 26,...

  10. Exploring Plasma Science Advances from Fusion Findings to Astrophysica...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Exploring Plasma Science Advances from Fusion Findings to Astrophysical Achievements By ... The latest advances in plasma physics were the focus of more than 1,000 scientists from ...

  11. EA-1762: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of No Significant Impact EA-1762: Finding of No Significant Impact Wellford Landfill Methane and Greenhouse Gas to Energy Project, Spartanburg County, South Carolina The...

  12. Rainbows and Leprechauns: Finding Gold in Partnerships (101)...

    Broader source: Energy.gov (indexed) [DOE]

    Peer Exchange Call Series: Rainbows and Leprechauns: Finding Gold in Partnerships (101), March 17, 2016, call slides and discussion summary. Call Slides and Discussion Summary ...

  13. EA-1837: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact Performance Verification Laboratory, Morgantown, West Virginia Based on the analyses in the environmental assessment, DOE determined that its...

  14. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of the Department of Energy, Waste Isolation Pilot Plant (WIPP) in Eddy County, New Mexico January 13, 2010 EA-1651: Finding of No Significant Impact U-233 Material...

  15. EA-1539: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Significant Impact EA-1539: Finding of No Significant Impact North Area Right-of-Way Maintenance, California Western Area Power Administration proposes to revise its operation and...

  16. Innovative Financing Solutions: Finding Money for Your Energy Efficiency Projects

    Broader source: Energy.gov [DOE]

    Provides an overview of how to find funding for your energy efficiency projects focusing on l-p agreements. Author: Energy Star

  17. EA-1974: Finding of No Significant Impact and Mitigation Action...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Bonneville Power Administration issued a finding of no significant impact (FONSI) for the proposed restoration of a tidal marsh in the Columbia River Estuary, near Astoria in ...

  18. EA-1696: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    EA-1696: Finding of No Significant Impact Pantex Renewable Energy Project, Amarillo, Texas The NNSA has prepared an Environmental Assessment to analyze the potential...

  19. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    and Heating Facilities at the Savannah River Site, South Carolina June 11, 2008 EA-1613: Finding of No Significant Impact Proposed High Explosive Pressing Facility Pantex...

  20. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of No Significant Impact Proposed Gas Main and Distribution System Upgrade, Pantex Plant, Amarillo, Texas August 26, 2005 EA-1542: Finding of No Significant Impact Burleigh County...

  1. Microsoft Word - Summary of RCA findings and lessons learned...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Work Planning and Scheduling System project Root cause analysis findings and lessons learned Summary BPA stopped the Work Planning and Scheduling System project in February 2012...

  2. EA-1384: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    The proposed action also involves the installation and operation of the Helios(High Energy Lithography Source) accelerator in the Free Electron Laser (FEL) addition. Finding of No ...

  3. EA-1499: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    EA-1499: Finding of No Significant Impact RadiologicalNuclear Countermeasures Test and Evaluation Complex, Nevada Test Site The National Nuclear Security Administration (NNSA) has ...

  4. Scientist finds new way to predict heat layer troublemaker |...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Scientist finds new way to predict heat layer troublemaker By John Greenwald August 27, ... But heat inevitably flows through the system and becomes separated, or scraped off, from ...

  5. DOE issues Finding of No Significant Impact for the Environmental...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    issues Finding of No Significant Impact for the Environmental Assessment on Resumption of Transient Testing of Nuclear Fuels and Materials at Idaho National Laboratory DOE issues ...

  6. Find Financing for Energy Efficiency Upgrades | Department of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Efficiency Upgrades Find Financing for Energy Efficiency Upgrades Photo of a block in lower downtown Denver that is part of Living City Block, a DOE Commercial Building ...

  7. EA-1628: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Iowa. PDF icon Mitigation Action Plan for the Environmental Assessment, Notice of Wetlands Involvement and Finding of No Significant Impact for Construction and Operation of a...

  8. FOIA-Summary of Findings: Circumstances surrounding decisions...

    Broader source: Energy.gov (indexed) [DOE]

    FOIA request FOIA-Summary of Findings: Circumstances surrounding decisions regarding the security clearance, access and work assignments (4.61 MB) More Documents & Publications ...

  9. EA-1796: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    near Gillespie, Arizona, DOEEA-1796 (February 2011) More Documents & Publications EA-1796: Final Environmental Assessment EA-1796: Finding of No Significant Impact MESQUITE

  10. EA-1952: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ...energy.govnode607866. Download Document PDF icon EA-1952: Finding of No Significant Impact More Documents & Publications EA-1952: Draft Environmental Assessment EA-1952: Final

  11. EA-1931: Finding of No Significant Impact and Mitigation Action...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Bonneville Power Administration issued a finding of no significant impact and a ... More Documents & Publications EA-1931: Draft Environmental Assessment EA-1931: Final ...

  12. EA-1979: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    icon EA-1979-FONSI-2015.pdf More Documents & Publications EA-1979: Draft Environmental Assessment EA-1979: Final Environmental Assessment EA-1955: Finding of No Significant Impact

  13. EA-1700: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    LBNLUniversity of California Berkeley programs that focus on computational and computer science research in a new facility on the LBNL site. PDF icon Finding of No Significant...

  14. Finding Large Aperture Fractures in Geothermal Resource Areas...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ...-Offset Surface Seismic Survey, PSInSAR, and Kinematic Analysis Finding Large Aperture Fractures in Geothermal Resource Areas Using A Three-Component Long-Offset Surface Seismic ...

  15. Findings of No Significant Impact (FONSI) | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    Ridge Reservation for the biological control of the emerald ash borer, a nonnative invasive beetle. May 1, 2015 EA-1796: Finding of No Significant Impact Loan Guarantee to...

  16. EA-1747: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    EA-1747: Finding of No Significant Impact Rocky Flats Surface Water Configuration, Jefferson County, Colorado Surface Water Configuration Project at the Rocky Flats Site, Colorado ...

  17. EA-1770: Finding of No Significant Impact | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    of the National Environmental Policy Act (NEPA). Finding of No Significant Impact Photovoltaic Solar Project at the Durango, Colorado, Disposal Site, La Plata County, DOEEA 1770...

  18. EA-1955: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Western Area Power Administration (Western) issued a finding of no significant impact for a proposal to interconnect, via a proposed new substation, a proposed Dakota Plains ...

  19. EA-1488: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    EA-1488: Finding of No Significant Impact U-233 Disposition, Medical Isotope Production, and Building 3019 Complex Shutdown at the Oak Ridge National Laboratory, Oak Ridge, ...

  20. EA-1929: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    EA-1929: Finding of No Significant Impact NorthStar Medical Technologies LLC, Commercial Domestic Production of the Medical Isotope Molybdenum-99 NNSA has completed the Final ...

  1. EA-1651: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    EA-1651: Final Environmental Assessment EA-1488: Finding of No Significant Impact EA-1488: Environmental Assessment for the U-233 Disposition, Medical Isotope Production, and ...

  2. EA-1535: Finding of No Significant Impact | Department of Energy

    Energy Savers [EERE]

    EA-1535: Finding of No Significant Impact Uranium Leasing Program U.S. Department of Energy Office of Legacy Management prepared the Uranium Leasing Program Programmatic ...

  3. Rainbows and Leprechauns: Finding Gold in Partnerships (101)

    Broader source: Energy.gov [DOE]

    Better Buildings Residential Network Peer Exchange Call Series: Rainbows and Leprechauns: Finding Gold in Partnerships (101), March 17, 2016, call slides and discussion summary.

  4. EA-1836: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    to facilitiate installation and operations of a high-efficiency natural-gas-fired cogeneration facility - would result in no significant adverse impacts. Finding of No...

  5. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    EA-1754: Finding of No Significant Impact Public Service Company of New Mexico, Photovoltaic Plus Battery for Simultaneous Voltage Smoothing and Peak Shifting Project,...

  6. EA-1611: Finding of No Significant Impact | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    finding of no significant impact (FONSI) for the proposed Colorado Highlands Wind Project.The FONSI describes the information Western used to determine that Western's proposal to...

  7. EA-1921: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact Silver Peak Area Geothermal Exploration Project, Esmeralda County, NV Based on the evaluations in the final EA and subject to the mitigations...

  8. EA-1801: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    EA-1801: Finding of No Significant Impact Granite Reliable Power Wind Project, Coos County, New Hampshire The Department of Energy is adopting an environmental assessment completed ...

  9. EA-1882: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Range Environmental Assessment, DOEEA-1882 (March 2011) More Documents & Publications EA-1822: Final Environmental Assessment EA-1984: Finding of No Significant Impact EA-1555...

  10. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    No Significant Impact Sunflower Wind Project, Hebron, North Dakota September 3, 2014 EA-1984: Finding of No Significant Impact Disposition of Five Signature Properties at Idaho...

  11. EA-1907: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Impact EA-1907: Finding of No Significant Impact Western Plains Energy, LLC Biogas Anaerobic Digester Facility, Oakley, Kansas Based on the analysis in the Final EA and...

  12. EA-1183: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    EA-1183: Finding of No Significant Impact Coal-fired Diesel Generator University of ... for demonstrating the performance of a coal-fired generator at the University of ...

  13. EA-1426: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    EA-1426: Finding of No Significant Impact Linac Coherent Light Source Project, Stanford ... Coherent Light Source (LCLS) at the Stanford Linear Accelerator Center (SLAC), ...

  14. EA-1613: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    EA-1613: Finding of No Significant Impact Proposed High Explosive Pressing Facility Pantex Plant, Amarillo, Texas The proposed action is to construct a new facility that would ...

  15. EA-1927: Finding of No Significant Impact | Department of Energy

    Energy Savers [EERE]

    EA-1927: Finding of No Significant Impact Paducah Gaseous Diffusion Plant Potential Land ... the Paducah Gaseous Diffusion Plant from DOE to other entities for economic development. ...

  16. Mound Plant Director's Final Findings and Orders, October 4,...

    Office of Environmental Management (EM)

    Director's Final Findings and Orders State Ohio Agreement Type Compliance Agreement Legal Driver(s) FFCAct Scope Summary Approve the Compliance Plan Volume of the amended PSTP, ...

  17. EA-1777: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Significant Impact EA-1777: Finding of No Significant Impact Lincoln Electric's Wind Energy Project in Euclid, OH DOE prepared an environmental assessment analyzing the potential...

  18. Sugarcane transgenics expressing MYB transcription factors show improved glucose release

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Poovaiah, Charleson R.; Bewg, William P.; Lan, Wu; Ralph, John; Coleman, Heather D.

    2016-07-15

    In this study, sugarcane, a tropical C4 perennial crop, is capable of producing 30-100 tons or more of biomass per hectare annually. The lignocellulosic residue remaining after sugar extraction is currently underutilized and can provide a significant source of biomass for the production of second-generation bioethanol. As a result, MYB31 and MYB42 were cloned from maize and expressed in sugarcane with and without the UTR sequences. The cloned sequences were 98 and 99 % identical to the published nucleotide sequences. The inclusion of the UTR sequences did not affect any of the parameters tested. There was little difference in plantmore » height and the number of internodes of the MYB-overexpressing sugarcane plants when compared with controls. MYB transgene expression determined by qPCR exhibited continued expression in young and maturing internodes. MYB31 downregulated more genes within the lignin biosynthetic pathway than MYB42. MYB31 and MYB42 expression resulted in decreased lignin content in some lines. All MYB42 plants further analyzed showed significant increases in glucose release by enzymatic hydrolysis in 72 h, whereas only two MYB31 plants released more glucose than control plants. This correlated directly with a significant decrease in acid-insoluble lignin. Soluble sucrose content of the MYB42 transgenic plants did not vary compared to control plants. In conclusion, this study demonstrates the use of MYB transcription factors to improve the production of bioethanol from sugarcane bagasse remaining after sugar extraction.« less

  19. Study Finds Potential New Biomarker for Cancer Patient Prognosis

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Study Finds Potential New Biomarker for Cancer Patient Prognosis A new study links the overexpression of 14 genes related to cell division to cancer patients' prognosis and response to specific treatments. The findings could be used to develop a biomarker used to make more informed decisions about cancer therapy. ← Previous

  20. Extracellular Matrix-Regulated Gene Expression RequiresCooperation of SWI/SNF and Transcription Factors

    SciTech Connect (OSTI)

    Xu, Ren; Spencer, Virginia A.; Bissell, Mina J.

    2006-05-25

    Extracellular cues play crucial roles in the transcriptional regulation of tissue-specific genes, but whether and how these signals lead to chromatin remodeling is not understood and subject to debate. Using chromatin immunoprecipitation (ChIP) assays and mammary-specific genes as models, we show here that extracellular matrix (ECM) molecules and prolactin cooperate to induce histone acetylation and binding of transcription factors and the SWI/SNF complex to the {beta}- and ?-casein promoters. Introduction of a dominant negative Brg1, an ATPase subunit of SWI/SNF complex, significantly reduced both {beta}- and ?-casein expression, suggesting that SWI/SNF-dependent chromatin remodeling is required for transcription of mammary-specific genes. ChIP analyses demonstrated that the ATPase activity of SWI/SNF is necessary for recruitment of RNA transcriptional machinery, but not for binding of transcription factors or for histone acetylation. Coimmunoprecipitation analyses showed that the SWI/SNF complex is associated with STAT5, C/EBP{beta}, and glucocorticoid receptor (GR). Thus, ECM- and prolactin-regulated transcription of the mammary-specific casein genes requires the concerted action of chromatin remodeling enzymes and transcription factors.

  1. EV Everywhere: Find Electric Vehicle Models | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Find Electric Vehicle Models EV Everywhere: Find Electric Vehicle Models Search Car: Year: -- ALL -- Make: -- ALL -- Market Class: -- ALL -- All-Electric Range: Min -- ALL -- 10 miles 20 miles 30 miles 40 miles 50 miles 60 miles 70 miles 80 miles 90 miles 100 miles 110 miles 120 miles 130+ miles Gasoline Back-Up Available: -- ALL -- No Yes Reset To find out if a plug-in electric vehicle (EV) will work for you, use the menus to the left to sort the available EV models on the market by year, make,

  2. EA-1772: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    The proposed action consists of constructing a road for limited year-round use with the ability for trucks traveling in opposite directions to pass. PDF icon Finding of No ...

  3. EA-1948: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    DOE's Western Area Power Administration issued a finding of no significant impact for a proposal to rebuild and upgrade two parallel 4.8-mile transmission lines between the Gila ...

  4. EA-1948: Revised Finding of No Significant Impact | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    On March 21, 2014, DOE's Western Area Power Administration issued a finding of no significant impact (FONSI) for a proposal to rebuild and upgrade two parallel 4.8-mile ...

  5. EA-1976: Finding of No Significant Impact | Department of Energy

    Energy Savers [EERE]

    EA-1976: Finding of No Significant Impact Emera CNG, LLC Compressed Natural Gas Project, Florida Based upon the EA, DOE has determined that the Emera CNG, LLC Compressed Natural ...

  6. Finding Six-Figure ROI From Energy Efficiency | Department of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Six-Figure ROI From Energy Efficiency Finding Six-Figure ROI From Energy Efficiency September 28, 2010 - 10:20am Addthis Kevin Craft What are the key facts? Recovery Act funded ...

  7. EA-1942: Finding of No Significant Impact | Department of Energy

    Energy Savers [EERE]

    DOE adopted FERC's EA and issued a Finding of No Significant Impact. PDF icon EA-1942-FONSIl2014.pdf More Documents & Publications Summary of LNG Export Applications of the Lower ...

  8. EA-1888: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of No Significant Impact EA-1888: Finding of No Significant Impact Old Town Fuel and Fiber Proposed Demonstration-Scale Integrated Biorefinery in Old Town, MN DOE has determined...

  9. EA-2002: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    EA-2002: Finding of No Significant Impact Right-of-Way Application for the Tucson-Apache 115-kV Transmission Line, Pima County, Arizona Western Area Power Administration issued a ...

  10. EA-1995: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    EA-1995: Finding of No Significant Impact Trestle Bay Ecosystem Restoration Project, Clatsop County, Oregon The U.S. Army Corps of Engineers prepared a Feasibility Study and EA ...

  11. Copper is Key in Burning Fat, New Study Finds

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Copper is Key in Burning Fat, New Study Finds A new study led by a Berkeley Lab scientist ... Researchers want to explore if a copper deficiency is linked to obesity and obesity ...

  12. Indoor Thermal Factors and Symptoms in Office Workers: Findings...

    Office of Scientific and Technical Information (OSTI)

    from the U.S. EPA BASE Study Citation Details In-Document Search Title: Indoor Thermal Factors and Symptoms in Office Workers: Findings from the U.S. EPA BASE Study You ...

  13. Portsmouth Decommissioning and Decontamination Project Director's Final Findings and Order

    Broader source: Energy.gov [DOE]

    Portsmouth Decommissioning and Decontamination (D&D) Project Director's Final Findings and Order defines the steps for identifying a range of technical alternatives for the D&D and waste...

  14. EA-1973: Finding of No Significant Impact and Mitigation Action...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    the project webpage. PDF icon EA-1973-FONSI-MAP-2015 More Documents & Publications EA-1973: Draft Environmental Assessment EA-1901: Finding of No Significant Impact EA-1518: Final

  15. Finding Large Aperture Fractures in Geothermal Resource Areas...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Long-Offset Surface Seismic Survey, PSInSAR, and Kinematic Analysis Finding Large Aperture Fractures in Geothermal Resource Areas Using A Three-Component Long-Offset Surface ...

  16. Unique Bioreactor Finds Algae's Sweet Spot - News Feature | NREL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Unique Bioreactor Finds Algae's Sweet Spot February 18, 2014 Close-up photo of a vial of green algae. Enlarge image Aeration helps algae grow and helps replicate real-life ...

  17. EA-1812: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    DOE has issued a Finding of No Significant Impact (FONSI) based on the information in the Final EA, FONSI, and the Supplement Analysis (SA) prepared to address changes in the ...

  18. EA-1964: FInding of No Significant Impact | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    13, DOE adopted the NSF EA and issued a finding of no significant impact regarding NEON Project research activities on the Oak Ridge Reservation. EA-1964-FONSI-2013.pdf More...

  19. EA-1711: Finding of No Significant Impact | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    America, Inc to construct and operate a high-volume manufacturing plant to make advanced lithium-ion cells and batteries, would result in no significant adverse impacts. Finding...

  20. DOE ZERH Webinar: Tour of Zero, Helping Customers Find Better...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Are you interested in helping homebuyers find a better home at a lower cost? The DOE Tour of ... This national campaign is where you come in. DOE needs Innovation partners vested in ...

  1. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Winnebago County, Illinois December 2, 2011 EA-1697: Finding of No Significant Impact Right-of-Way Maintenance in the San Joaquin Valley, California November 10, 2011 EA-1862:...

  2. EA-1941: Finding of No Significant Impact (FONSI) | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    BPA issued a Finding of No Significant Impact for proposed improvements to 13 miles of access roads for its existing 115-kV Boyer-Tillamook No. 1 Transmission Line. PDF icon ...

  3. Scientists Find Asymmetry in Topological Insulators - News Releases | NREL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Scientists Find Asymmetry in Topological Insulators Surprising findings bolster case for energy efficient quantum computer August 12, 2013 New research shows that a class of materials being eyed for the next generation of computers behaves asymmetrically at the sub-atomic level. This research is a key step toward understanding the topological insulators that may have the potential to be the building blocks of a super-fast quantum computer that could run on almost no electricity. Scientists from

  4. Moving Industry Forward: Finding the Environmental Opportunity in Biochar

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Moving Industry Forward: Finding the Environmental Opportunity in Biochar Moving Industry Forward: Finding the Environmental Opportunity in Biochar Print Thursday, 12 September 2013 08:41 The Environmental Protection Agency (EPA) has been investigating biofuels for several years now as part of its mission of moving the nation toward a cleaner energy future. Recently, EPA scientists have refocused their research on a promising biofuel byproduct-biochar, which is the solid material that's left

  5. Neutrons find "missing" magnetism of plutonium

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Neutrons find "missing" magnetism of plutonium Neutrons find "missing" magnetism of plutonium Groundbreaking work at two Department of Energy national laboratories has confirmed plutonium's magnetism, which scientists have long theorized but have never been able to experimentally observe. July 10, 2015 Doug Abernathy, left, ARCS instrument scientist at Oak Ridge National Laboratory, and Marc Janoschek, Los Alamos National Laboratory, prepare their sample for experiments at

  6. Two Independent Assessments Find the Department of Energy's Yucca Mountain

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Project is on Track | Department of Energy Independent Assessments Find the Department of Energy's Yucca Mountain Project is on Track Two Independent Assessments Find the Department of Energy's Yucca Mountain Project is on Track December 13, 2007 - 4:44pm Addthis WASHINGTON, DC - The U.S. Department of Energy (DOE) Director of the Office of Civilian Radioactive Waste Management (OCRWM) today released two independent assessments addressing areas critical to the overall success of the Yucca

  7. DOE Issues Finding of No Significant Impact and Final Environmental

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Assessment on Road Project U.S. DEPARTMENT OF ENERGY IDAHO FALLS, IDAHO, 83403 News Media Contact: Tim Jackson (208) 526-8484 For Immediate Release Thursday August 12, 2010 DOE Issues Finding of No Significant Impact and Final Environmental Assessment on Road Project The U.S. Department of Energy has issued a Finding of No Significant Impact stemming from an environmental assessment it performed to examine the potential environmental impacts of building an internal road to safely ship

  8. EA-1744: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1744: Finding of No Significant Impact Brea Power II, LLC's Olinda Combined Cycle Electric Generating Plant Fueled by Waste Landfill Gas, Brea, California Based in the analyses in the Environmental Assessment, DOE determined that its proposed action - awarding a grant to Brea Power II, LLC to facilitate modifcation and expansion of an existing landfill gas collection system and construction and operation of a combined cycle power facility at the Olinda Alpha

  9. EA-2000: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-2000: Finding of No Significant Impact Proposed Land Transfer to Develop a General Aviation Airport at the East Tennessee Technology Park Heritage Center, Oak Ridge, Tennessee Oak Ridge Office of Environmental Management issued a FONSI for the proposed title transfer of property located at the East Tennessee Technology Park (ETTP) Heritage Center to the Metropolitan Knoxville Airport Authority for the purpose of constructing and operating a general aviation

  10. EA-2005: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-2005: Finding of No Significant Impact Chromium Plume Control Interim Measure and Plume-Center Characterization, Los Alamos National Laboratory, Los Alamos, NM Based upon the review of the EA prepared by DOE pursuant to the requirements of the National Environmental Policy Act (NEPA), DOE has determined that the planned interim measure to control chromium plume migration while long-term corrective action remedies are evaluated and implemented would not be a

  11. EA-2005: Floodplain Statement of Findings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Floodplain Statement of Findings EA-2005: Floodplain Statement of Findings Chromium Plume Control Interim Measure and Plume-Center Characterization, Los Alamos National Laboratory, Los Alamos, NM The Department of Energy Environmental Management Los Alamos Field Office issued a Final Environmental Assessment for the Chromium Plume Control Interim Measure and Plume-Center Characterization at the Los Alamos National Laboratory, Los Alamos, New Mexico (DOE/EA-2005). A Floodplain Assessment was

  12. EA-2013: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    3: Finding of No Significant Impact EA-2013: Finding of No Significant Impact Herbicide Application at Three Substations; Imperial County (California), Maricopa and Yuma Counties (Arizona) Western Area Power Administration (Western) issued a FONSI for Western's proposed use of selected herbicides for the treatment of undesirable vegetation within three existing substations on lands administered by the Bureau of Land Management. For more information on this project, see:

  13. EA-2014: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    4: Finding of No Significant Impact EA-2014: Finding of No Significant Impact Y-12 National Security Complex Emergency Operations Center; Oak Ridge, TN Based upon the EA, the National Nuclear Security Administration (NNSA) has determined that the Y-12 Emergency Operations Center project would not be a major Federal action significantly affecting the quality of the human environment within the meaning of NEPA. Therefore, an environmental impact statement is not required. For more information on

  14. How to Find EERE Funding Opportunity Announcements | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    How to Find EERE Funding Opportunity Announcements How to Find EERE Funding Opportunity Announcements Most EERE awards are selected competitively through funding opportunity announcements (FOAs) and solicitations. Competition ensures that EERE selects awards based on merit, which results in better award outcomes. All FOAs, both open and closed, are posted on two primary websites. Graphic of two circles that connect with grants.gov and EERE Exchange text. Grants.gov Grants.gov is a comprehensive

  15. Finding Transition Pathways Using the String Method with Swarms of

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Trajectories | Argonne Leadership Computing Facility Finding Transition Pathways Using the String Method with Swarms of Trajectories Authors: Pan, A.C., Sezer, D., Roux, B. An approach to find transition pathways in complex systems is presented. The method, which is related to the string method in collective variables of Maragliano et al. (J. Chem. Phys. 2006, 125, 024106), is conceptually simple and straightforward to implement. It consists of refining a putative transition path in the

  16. Finding the Right Filling Station for Alternative Vehicles Now Easier

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Finding the Right Filling Station for Alternative Vehicles Now Easier For more information contact: e:mail: Public Affairs Golden, Colo., May 5, 1999 — A new online interactive computer program is taking the guesswork out of finding the fuel needed by the thousands of alternative vehicles on the road today in the United States. The program, called the Alternative Fuel Refueling Station Locator, was developed by the U.S. Department of Energy's (DOE) Alternative Fuels Data Center (AFDC). The AFDC

  17. Finding Large Aperture Fractures in Geothermal Resource Areas Using A

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Three-Component Long-Offset Surface Seismic Survey, PSInSAR, and Kinematic Analysis | Department of Energy Finding Large Aperture Fractures in Geothermal Resource Areas Using A Three-Component Long-Offset Surface Seismic Survey, PSInSAR, and Kinematic Analysis Finding Large Aperture Fractures in Geothermal Resource Areas Using A Three-Component Long-Offset Surface Seismic Survey, PSInSAR, and Kinematic Analysis Fining Large Aperture Fractures in Geothermal Resource Areas Using A

  18. Finding and Winning Simplified Acquisitions to Grow Your Business

    Office of Environmental Management (EM)

    Energy Products & Technologies » Energy-Efficient Products » Find Product Categories Covered by Efficiency Programs Find Product Categories Covered by Efficiency Programs Legend ★ ENERGY STAR △ FEMP Designated O EPEAT ♦ Low Standby Power X WaterSense Federal agencies are required to purchase energy-efficient products. To help buyers meet these requirements, the Federal Energy Management Program (FEMP) maintains acquisition guidance for the following product categories, which are

  19. EA-1236: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1236: Finding of No Significant Impact Preparation for Transfer of Ownership of Naval Petroleum Reserve No. 3, Natrona County, Wyoming Based on the analysis of the Sitewide Environmental Assessment for Naval Petroleum Reserve No. 3 (NPR-3) DOE has determined that the proposed action to conduct activities in anticipation of possible transfer of NPR-3 out of Federal operation is not a major Federal action significantly affecting the quality of the human

  20. EA-1321: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1321: Finding of No Significant Impact Proposed Upgrade and Improvement of The National Synchrotron Light Source Complex at Brookhaven National Laboratory, Upton, New York Based on the analysis in the EA, DOE has determined that the continued operation, construction, and upgrades of the National Synchrotron Light Source Complex at Brookhaven National Laboratory do not constitute a major Federal action significantly affecting the quality of the human

  1. EA-1796: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1796: Finding of No Significant Impact Loan Guarantee to Sempra Generation for Construction of the Mesquite Solar Energy Project; Maricopa County, Arizona Summary Summary DOE's Western Area Power Administration issued a FONSI for the power purchase agreement, on behalf of the Navy, for renewable energy that would be generated by the Mesquite Solar Energy Project. Documents Available For Download EA-1796 FONSI 2015 (1.29 MB) More Documents & Publications

  2. EA-1891: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    891: Finding of No Significant Impact EA-1891: Finding of No Significant Impact Alvey-Fairview Transmission Line Rebuild Project, Oregon Based on the information in the EA, BPA determined that the proposed action is not a major federal action significantly affecting the quality of the human environment within the meaning of NEPA. Therefore, an EIS will not be prepared and BPA is issuing this FONSI for the proposed action. EA-1891-FONSI-2014.pdf (244.47 KB) More Documents & Publications

  3. EA-1245: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    245: Finding of No Significant Impact EA-1245: Finding of No Significant Impact Kotzebue Wind Installation Project, Kotzebue, Alaska The U.S. Department of Energy's Golden Field Office is considering providing financial assistance to the Kotzebue Electric Association to expand its existing wind installation site near Kotzebue, Alaska. Like many rural Alaskan towns, Kotzebue uses diesel-powered generators to produce electricity, the high cost of which is currently subsidized by the Alaskan State

  4. EA-1985: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1985: Finding of No Significant Impact Virginia Offshore Wind Technology Advancement Project on the Atlantic Outer Continental Shelf Offshore Virginia DOE is proposing to fund Virginia Electric and Power Company's Virginia Offshore Wind Technology Advancement Project (VOWTAP). The proposed VOWTAP project consists of design, construction and operation of a 12 megawatt offshore wind facility located approximately 24 nautical miles off the coast of Virginia

  5. EA-2001: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-2001: Finding of No Significant Impact Energy Efficiency Standards for New Federal Commercial and Multi-Family High-Rise Residential Buildings' Baseline Standards Update (RIN 1904-AD39) Based upon the EA, DOE has determined that revising the Federal building energy efficiency standards for commercial and multi-family high-rise residential buildings to ASHRAE 90.1-2013 would not be a major Federal action significantly affecting the quality of the human

  6. EA-2004: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-2004: Finding of No Significant Impact The Seneca Nation Wind Turbine Project, Cattaraugus Territory, Erie County, New York Based upon the EA, DOE has determined that authorizing the expenditure of federal funding to the Seneca Nation of Indians to design, permit, and construct up to a 2.0 megawatt wind turbine on Tribal common lands in the Cattaraugus Territory, New York would not be a major Federal action significantly affecting the quality of the human

  7. EA-2017: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-2017: Finding of No Significant Impact Braddock Locks and Dam Hydro Electric Project Based upon the review of the EA prepared by the Federal Energy Regulatory Commission (FERC) and adopted by DOE pursuant to the requirements of the National Environmental Policy Act (NEPA), DOE has determined that authorizing the expenditure of federal funding to Hydro Green Energy, LLC to fabricate, install, and operate one interchangeable Modular Bulb Turbine (MBT), which

  8. CREED: Helping Colorado Cleantech Startups Find the Money - News Releases |

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    NREL CREED: Helping Colorado Cleantech Startups Find the Money January 19, 2012 The Colorado Center for Renewable Energy Economic Development (CREED) at the U.S. Department of Energy's National Renewable Energy Laboratory (NREL) invites cleantech entrepreneurs to attend the next event in its Entrepreneur Series. The Jan. 26 class, "Financing Your Cleantech Startup," will provide cleantech entrepreneurs with an overview of how to find capital for a startup. "If you are in

  9. NERSC Resources Help Find Roots of MJO Modeling Mismatches

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    NERSC Resources Help Find Roots of MJO Modeling Mismatches NERSC Resources Help Find Roots of MJO Modeling Mismatches April 18, 2016 MJOmodeling From Calcutta, India, to Seattle, Wash. and beyond, the MJO can bring storms and plenty of rain in wet years. PNNL researchers are using field data and NERSC supercomputers to better model how the MJO operates and understand its interaction regional weather systems around the world. Image: PNNL An Alaska-sized pulse of clouds and precipitation

  10. Curiosity rover finds evidence of Mars' primitive continental crust

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Curiosity rover finds evidence of Mars' primitive continental crust Curiosity rover finds evidence of Mars' primitive continental crust This is the first discovery of a potential "continental crust" on Mars. July 13, 2015 Igneous clast named Harrison embedded in a conglomerate rock in Gale crater, Mars, shows elongated light-toned feldspar crystals. The mosaic merges an image from Mastcam with higher-resolution images from ChemCam's Remote Micro-Imager. Credit:

  11. EA-1655: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    5: Finding of No Significant Impact EA-1655: Finding of No Significant Impact The Berkeley Lab Laser Accelerator (BELLA) Laser Acquisition, Installation and Use for Research and Development The Proposed Action would create and operate an experimental facility for further advancing the development of laser-driven, plasma-based, particle beam accelerators. An existing, approximately 7,000-square-foot, accelerator laboratory area inside Building 71 at Lawrence Berkeley National Laboratory (LBNL)

  12. Study finds surprising variability in shape of Van Allen Belts

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Study finds surprising variability in shape of Van Allen Belts Study finds surprising variability in shape of Van Allen Belts Understanding the shape and size of the belts, which shrink and swell in response to magnetic storms coming from the sun, is crucial for protecting our technology in space. February 23, 2016 1. The traditional idea of the radiation belts includes a larger, more dynamic outer belt and a smaller, more stable inner belt with an empty slot region separating the two. However,

  13. DOE issues Finding of No Significant Impact for the Environmental

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Assessment on Resumption of Transient Testing of Nuclear Fuels and Materials at Idaho National Laboratory | Department of Energy issues Finding of No Significant Impact for the Environmental Assessment on Resumption of Transient Testing of Nuclear Fuels and Materials at Idaho National Laboratory DOE issues Finding of No Significant Impact for the Environmental Assessment on Resumption of Transient Testing of Nuclear Fuels and Materials at Idaho National Laboratory February 28, 2014 - 12:00pm

  14. DOE issues Finding of No Significant Impact for the Environmental

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Assessment on Resumption of Transient Testing of Nuclear Fuels and Materials at Idaho National Laboratory | Department of Energy DOE issues Finding of No Significant Impact for the Environmental Assessment on Resumption of Transient Testing of Nuclear Fuels and Materials at Idaho National Laboratory DOE issues Finding of No Significant Impact for the Environmental Assessment on Resumption of Transient Testing of Nuclear Fuels and Materials at Idaho National Laboratory February 28, 2014 -

  15. Transcript of April 11, 2013, TAP webinar titled Financing Energy Upgrades for K-12 School Districts

    Broader source: Energy.gov [DOE]

    U.S. Department of Energy (DOE) Technical Assistance Program (TAP) transcript of a TAP webinar held on April 11, 2013 and dealing with how to finance energy efficiency upgrades for K-12 school districts.

  16. Impact of the N-Terminal Domain of STAT3 in STAT3-Dependent Transcript...

    Office of Scientific and Technical Information (OSTI)

    Impact of the N-Terminal Domain of STAT3 in STAT3-Dependent Transcriptional Activity Citation Details In-Document Search Title: Impact of the N-Terminal Domain of STAT3 in ...

  17. Transcript of March 28, 2013, TAP webinar titled Internal Benchmarking Outreach and Data Collection Techniques

    Office of Energy Efficiency and Renewable Energy (EERE)

    U.S. Department of Energy (DOE) Technical Assistance Program (TAP) transcript of a TAP webinar held on March 28, 2013 and dealing with internal benchmarking outreach and data collection techniques.

  18. Transcriptional program for nitrogen starvation-induced lipid accumulation in Chlamydomonas reinhardtii

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Garcia de Lomana, Adrian Lopez; Schäuble, Sascha; Valenzuela, Jacob; Imam, Saheed; Carter, Warren; Bilgin, Damla D.; Yohn, Christopher B.; Turkarslan, Serdar; Reiss, David J.; Orellana, Monica V.; et al

    2015-12-02

    Algae accumulate lipids to endure different kinds of environmental stresses including macronutrient starvation. Although this response has been extensively studied, an in depth understanding of the transcriptional regulatory network (TRN) that controls the transition into lipid accumulation remains elusive. In this study, we used a systems biology approach to elucidate the transcriptional program that coordinates the nitrogen starvation-induced metabolic readjustments that drive lipid accumulation in Chlamydomonas reinhardtii. We demonstrate that nitrogen starvation triggered differential regulation of 2147 transcripts, which were co-regulated in 215 distinct modules and temporally ordered as 31 transcriptional waves. An early-stage response was triggered within 12 minmore » that initiated growth arrest through activation of key signaling pathways, while simultaneously preparing the intracellular environment for later stages by modulating transport processes and ubiquitin-mediated protein degradation. Subsequently, central metabolism and carbon fixation were remodeled to trigger the accumulation of triacylglycerols. Further analysis revealed that these waves of genome-wide transcriptional events were coordinated by a regulatory program orchestrated by at least 17 transcriptional regulators, many of which had not been previously implicated in this process. We demonstrate that the TRN coordinates transcriptional downregulation of 57 metabolic enzymes across a period of nearly 4 h to drive an increase in lipid content per unit biomass. Notably, this TRN appears to also drive lipid accumulation during sulfur starvation, while phosphorus starvation induces a different regulatory program. The TRN model described here is available as a community-wide web-resource at http://networks.systemsbiology.net/chlamy-portal. In conclusion, in this work, we have uncovered a comprehensive mechanistic model of the TRN controlling the transition from N starvation to lipid

  19. Transcript of How to Navigate the New and Improved State and Local Solution

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Center Webinar | Department of Energy Transcript of How to Navigate the New and Improved State and Local Solution Center Webinar Transcript of How to Navigate the New and Improved State and Local Solution Center Webinar So, starting with Weatherization and Intergovernmental Programs, who we are and what we do, our mission is primarily to accelerate the deployment of energy efficiency and renewable energy technologies, working primarily with our stakeholders, state and local governments.

  20. DOE Zero Energy Ready Home: Better Business for Builders Webinar Transcript

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    | Department of Energy Home: Better Business for Builders Webinar Transcript DOE Zero Energy Ready Home: Better Business for Builders Webinar Transcript Below is the text version of the webinar, DOE Zero Energy Ready Home: Better Business for Builders, presented in August 2014. GoToWebinar voice: The broadcast is now starting. All attendees are in listen- only mode. Lindsay Parker: ... here today, and welcome to the Department of Energy Zero Energy Ready Home Technical Training Webinar

  1. Curved track segment finding using Tiny Triplet Finder (TTF)

    SciTech Connect (OSTI)

    Wu, Jin-Yuan; Wang, M.; Gottschalk, E.; Shi, Z.; /Fermilab

    2006-11-01

    We describe the applications of a track segment recognition scheme called the Tiny Triplet Finder (TTF) that involves the grouping of three hits satisfying a constraint forming of a track segment. The TTF was originally developed solving straight track segment finding problem, however, it is also suitable in many curved track segment finding problems. The examples discussed in this document are among popular detector layouts in high-energy/nuclear physics experiments. Although it is not practical to find a universal recipe for arbitrary detector layouts, the method of the TTF application is illustrated via the discussion of the examples. Generally speaking, whenever the data item to be found in a pattern recognition problem contains two free parameters, and if the constraint connecting the measurements and the two free parameters has an approximate shift invariant property, the Tiny Triplet Finder can be used.

  2. Research Finds Vitamin D Deficiency Affects Bone Quality

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Research Finds Vitamin D Deficiency Affects Bone Quality Research Finds Vitamin D Deficiency Affects Bone Quality Print Wednesday, 30 April 2014 00:00 Vitamin D deficiency is a widespread medical condition that plays a major role in human bone health. Scientists know that a lack of vitamin D can cause bone diseases such as rickets and osteomalacia. Now a team of researchers working at the ALS has also found that vitamin D deficiency plays a significant role in the bone-aging process. Low levels

  3. Finding the needle in the haystack: Algorithms for conformational optimization

    SciTech Connect (OSTI)

    Andricioaei, I.; Straub, J.E.

    1996-09-01

    Algorithms are given for comformational optimization of proteins. The protein folding problems is regarded as a problem of global energy mimimization. Since proteins have hundreds of atoms, finding the lowest-energy conformation in a many-dimensional configuration space becomes a computationally demanding problem.{copyright} {ital American Institute of Physics.}

  4. Finding and Winning Simplified Acquisitions, Part 1 | Department of Energy

    Energy Savers [EERE]

    Internships Find Internships Internships in energy efficiency and renewable energy-related fields, such as solar and fuel cells, energy efficiency, atmospheric sciences, ecology, global carbon cycles, climatology, and more, include EERE's own student internship program. Featured Internships Clean Cities University Workforce Development Program Clean Cities logo Science Undergraduate Laboratory Internships (SULI) Fall 2013 Picture of tow people in a labratory working Minority Educational

  5. Finding Chemical Reaction Paths with a Multilevel Preconditioning Protocol

    SciTech Connect (OSTI)

    Kale, Seyit; Sode, Olaseni; Weare, Jonathan; Dinner, Aaron R.

    2014-11-07

    Finding transition paths for chemical reactions can be computationally costly owing to the level of quantum-chemical theory needed for accuracy. Here, we show that a multilevel preconditioning scheme that was recently introduced (Tempkin et al. J. Chem. Phys. 2014, 140, 184114) can be used to accelerate quantum-chemical string calculations. We demonstrate the method by finding minimum-energy paths for two well-characterized reactions: tautomerization of malonaldehyde and Claissen rearrangement of chorismate to prephanate. For these reactions, we show that preconditioning density functional theory (DFT) with a semiempirical method reduces the computational cost for reaching a converged path that is an optimum under DFT by several fold. In conclusion, the approach also shows promise for free energy calculations when thermal noise can be controlled.

  6. Finding Chemical Reaction Paths with a Multilevel Preconditioning Protocol

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Kale, Seyit; Sode, Olaseni; Weare, Jonathan; Dinner, Aaron R.

    2014-11-07

    Finding transition paths for chemical reactions can be computationally costly owing to the level of quantum-chemical theory needed for accuracy. Here, we show that a multilevel preconditioning scheme that was recently introduced (Tempkin et al. J. Chem. Phys. 2014, 140, 184114) can be used to accelerate quantum-chemical string calculations. We demonstrate the method by finding minimum-energy paths for two well-characterized reactions: tautomerization of malonaldehyde and Claissen rearrangement of chorismate to prephanate. For these reactions, we show that preconditioning density functional theory (DFT) with a semiempirical method reduces the computational cost for reaching a converged path that is an optimum undermore » DFT by several fold. In conclusion, the approach also shows promise for free energy calculations when thermal noise can be controlled.« less

  7. NREL Scientists Find Key Function for Ferredoxins in Algae Hydrogen

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Production - News Releases | NREL Scientists Find Key Function for Ferredoxins in Algae Hydrogen Production Two of six iron-rich proteins shown to have role in algae metabolism; discovery could lead to enhanced hydrogen production February 10, 2014 Scientists at the Energy Department's National Renewable Energy Laboratory have demonstrated that just two of six iron-sulfur-containing ferredoxins in a representative species of algae promote electron transfers to and from hydrogenases. The

  8. NREL analysis finds tax credit extensions can impact renewable energy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    deployment and electric sector CO2 emissions - News Releases | NREL analysis finds tax credit extensions can impact renewable energy deployment and electric sector CO2 emissions February 22, 2016 The Energy Department's National Renewable Energy Laboratory (NREL) today released new analysis exploring the potential impact of recently extended federal tax credits on the deployment of renewable generation technologies and related U.S. electric sector carbon dioxide (CO2) emissions. The report,

  9. Fermilab | Science | Particle Physics 101 | How to find the smallest

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    particles - Particle Detectors How to find the smallest particles - Particle Detectors Scientists have invented and built many types of devices to detect particles. Most experiments at Fermilab use huge detectors that consist of intricate configurations of different detection devices, designed to collect the most possible information about the different types of particles created in a particle collision. Different types of particle detectors: Scintillators - When a high energy particle

  10. Finding renewable energy materials using one screensaver at a time:

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Combinatorial quantum chemistry for organic photovoltaics | MIT-Harvard Center for Excitonics Finding renewable energy materials using one screensaver at a time: Combinatorial quantum chemistry for organic photovoltaics April 24, 2012 at 3pm/36-428 Alan Aspuru-Guzik Department of Chemistry and Chemical Biology, Harvard University Alan_Aspuru-Guzik-Harvard-2_000 Abstract: During this talk, I will describe our group's efforts in the Clean Energy Project (http://cleanenergy.harvard.edu) , a

  11. Researchers find potential key for unlocking biomass energy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Unlocking biomass energy Researchers find potential key for unlocking biomass energy Potential pretreatment method that can make plant cellulose five times more digestible by enzymes that convert it into ethanol, a useful biofuel. July 20, 2011 Los Alamos National Laboratory sits on top of a once-remote mesa in northern New Mexico with the Jemez mountains as a backdrop to research and innovation covering multi-disciplines from bioscience, sustainable energy sources, to plasma physics and new

  12. FOR THE SUPPLEMENTAL ENVIRONMENTAL ASSESSMENT/FINDING OF NO

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    2 FOR THE SUPPLEMENTAL ENVIRONMENTAL ASSESSMENT/FINDING OF NO SIGNIFICANT IMPACT FOR THE UNIVERSITY OF MAINE'S DEEPWATER OFFSHORE FLOATING WIND TURBINE TESTING AND DEMONSTRATION PROJECT CASTINE, MAINE DOE/EA-1792-S1 US Department of Energy Office of Energy Efficiency and Renewable Energy Golden, Colorado November 2014 Supplement Analysis - 2 i November 20, 2014 DOE/EA-1792-S1 CONTENTS 1.0 Background

  13. Finding and Winning Simplified Acquisitions to Grow Your Business

    Office of Environmental Management (EM)

    Find Simplified Acquisitions... What They Buy. How Much They Spend. Which 'slices' of the Simplified pie can your company pursue? More than half of all Simplified Acquisition obligations went to small business concerns, set-aside or not! FSS (Federal Supply Schedule) *Multiple Award, Open Solicitation A simplified process of acquiring commonly used supplies and services in varying quantities at lower prices while obtaining discounts associated with volume buying. GSA and VA are the only

  14. Finding Solutions to Solar's Soft Cost Dilemma | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Solutions to Solar's Soft Cost Dilemma Finding Solutions to Solar's Soft Cost Dilemma January 8, 2013 - 1:22pm Addthis Brian Webster installs rooftop solar panels on a home in Englewood, Colorado. The Energy Department is working to streamline rooftop solar installations so that its faster, easier and cheaper for Americans to go solar. | Photo courtesy of Dennis Schroeder, NREL. Brian Webster installs rooftop solar panels on a home in Englewood, Colorado. The Energy Department is working to

  15. DOE issues Finding of No Significant Impact for the Environmental

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Assessment on Resumption of Transient Testing of Nuclear Fuels and Materials at Idaho National Laboratory Tim Jackson, (208) 526-8484 For Immediate Release: February 28, 2014 DOE issues Finding of No Significant Impact for the Environmental Assessment on Resumption of Transient Testing of Nuclear Fuels and Materials at Idaho National Laboratory The U.S. Department of Energy (DOE) has determined that resuming transient testing of nuclear fuels and materials at Idaho National Laboratory (INL)

  16. Summer interns present research findings in poster session | Princeton

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Plasma Physics Lab Summer interns present research findings in poster session By Raphael Rosen August 31, 2015 Tweet Widget Google Plus One Share on Facebook New Jersey Assemblywoman Donna Simon Talks With SULI Intern Cara Bagley (Photo by Elle Starkman) New Jersey Assemblywoman Donna Simon Talks With SULI Intern Cara Bagley Gallery: Physicist Charles Skinner and Intern Amanda Lewis (Photo by Elle Starkman) Physicist Charles Skinner and Intern Amanda Lewis Interns Matthew Lotocki, Zack

  17. EA-1815: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    5: Diamond Green Diesel Facility in Norco, LA EA-1795: Diamond Green Diesel Facility in Norco, LA April 1, 2011 EA-1795: Final Environmental Assessment Loan Guarantee to Diamond Green Diesel, LLC for Construction of the Diamond Green Diesel Facility in Norco, Louisiana April 18, 2011 EA-1795: Finding of No Significant Impact Loan Guarantee to Diamond Green Diesel for Construction of the Diamond Green Diesel Facility in Norco, Louisiana

    Final Environmental Assessment EA-1795: Final

  18. rVISTA for Comparative Sequence-Based Discovery of Functional Transcription Factor Binding Sites

    SciTech Connect (OSTI)

    Loots, Gabriela G.; Ovcharenko, Ivan; Pachter, Lior; Dubchak, Inna; Rubin, Edward M.

    2002-03-08

    Identifying transcriptional regulatory elements represents a significant challenge in annotating the genomes of higher vertebrates. We have developed a computational tool, rVISTA, for high-throughput discovery of cis-regulatory elements that combines transcription factor binding site prediction and the analysis of inter-species sequence conservation. Here, we illustrate the ability of rVISTA to identify true transcription factor binding sites through the analysis of AP-1 and NFAT binding sites in the 1 Mb well-annotated cytokine gene cluster1 (Hs5q31; Mm11). The exploitation of orthologous human-mouse data set resulted in the elimination of 95 percent of the 38,000 binding sites predicted upon analysis of the human sequence alone, while it identified 87 percent of the experimentally verified binding sites in this region.

  19. 10 CFR 850 Notice of Proposed Rulemaking Public Hearing Transcript, Richland, Washington – June 28-30, 2016

    Broader source: Energy.gov [DOE]

    Transcript of the Richland, WA public hearings to received comments on the proposed amendments to 10 CFR 850, Chronic Beryllium Disease Prevention Program.

  20. Scientist finds new way to predict heat layer troublemaker | Princeton

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Plasma Physics Lab Boon to fusion: Scientist finds new way to predict heat layer troublemaker By John Greenwald August 27, 2012 Tweet Widget Google Plus One Share on Facebook Rob Goldston. (Photo by Elle Starkman, PPPL Office Of Communications) Rob Goldston. Researchers at a recent worldwide conference on fusion power have confirmed the surprising accuracy of a new model for predicting the size of a key barrier to fusion that a top scientist at the U.S. Department of Energy's Princeton

  1. Scientist finds new way to predict heat layer troublemaker | Princeton

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Plasma Physics Lab Scientist finds new way to predict heat layer troublemaker By John Greenwald August 27, 2012 Tweet Widget Google Plus One Share on Facebook Researchers at a recent worldwide conference on fusion power have confirmed the surprising accuracy of a new model for predicting the size of a key barrier to fusion that a top scientist at the U.S. Department of Energy's Princeton Plasma Physics Laboratory (PPPL) has developed. The model could serve as a starting point for overcoming

  2. Research Finds Vitamin D Deficiency Affects Bone Quality

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Research Finds Vitamin D Deficiency Affects Bone Quality Print Vitamin D deficiency is a widespread medical condition that plays a major role in human bone health. Scientists know that a lack of vitamin D can cause bone diseases such as rickets and osteomalacia. Now a team of researchers working at the ALS has also found that vitamin D deficiency plays a significant role in the bone-aging process. Low levels of vitamin D, the "sunshine vitamin," have been previously linked to the

  3. Research Finds Vitamin D Deficiency Affects Bone Quality

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Research Finds Vitamin D Deficiency Affects Bone Quality Print Vitamin D deficiency is a widespread medical condition that plays a major role in human bone health. Scientists know that a lack of vitamin D can cause bone diseases such as rickets and osteomalacia. Now a team of researchers working at the ALS has also found that vitamin D deficiency plays a significant role in the bone-aging process. Low levels of vitamin D, the "sunshine vitamin," have been previously linked to the

  4. Research Finds Vitamin D Deficiency Affects Bone Quality

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Research Finds Vitamin D Deficiency Affects Bone Quality Print Vitamin D deficiency is a widespread medical condition that plays a major role in human bone health. Scientists know that a lack of vitamin D can cause bone diseases such as rickets and osteomalacia. Now a team of researchers working at the ALS has also found that vitamin D deficiency plays a significant role in the bone-aging process. Low levels of vitamin D, the "sunshine vitamin," have been previously linked to the

  5. Research Finds Vitamin D Deficiency Affects Bone Quality

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Research Finds Vitamin D Deficiency Affects Bone Quality Print Vitamin D deficiency is a widespread medical condition that plays a major role in human bone health. Scientists know that a lack of vitamin D can cause bone diseases such as rickets and osteomalacia. Now a team of researchers working at the ALS has also found that vitamin D deficiency plays a significant role in the bone-aging process. Low levels of vitamin D, the "sunshine vitamin," have been previously linked to the

  6. Research Finds Vitamin D Deficiency Affects Bone Quality

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Research Finds Vitamin D Deficiency Affects Bone Quality Print Vitamin D deficiency is a widespread medical condition that plays a major role in human bone health. Scientists know that a lack of vitamin D can cause bone diseases such as rickets and osteomalacia. Now a team of researchers working at the ALS has also found that vitamin D deficiency plays a significant role in the bone-aging process. Low levels of vitamin D, the "sunshine vitamin," have been previously linked to the

  7. Research Finds Vitamin D Deficiency Affects Bone Quality

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Research Finds Vitamin D Deficiency Affects Bone Quality Print Vitamin D deficiency is a widespread medical condition that plays a major role in human bone health. Scientists know that a lack of vitamin D can cause bone diseases such as rickets and osteomalacia. Now a team of researchers working at the ALS has also found that vitamin D deficiency plays a significant role in the bone-aging process. Low levels of vitamin D, the "sunshine vitamin," have been previously linked to the

  8. Finding Large Aperture Fractures in Geothermal Resource Areas Using A

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Three-Component Long-Offset Surface Seismic Survey, PSInSAR, and Kinematic Analysis | Department of Energy A Three-Component Long-Offset Surface Seismic Survey, PSInSAR, and Kinematic Analysis Finding Large Aperture Fractures in Geothermal Resource Areas Using A Three-Component Long-Offset Surface Seismic Survey, PSInSAR, and Kinematic Analysis Fining Large Aperture Fractures in Geothermal Resource Areas Using A Three-Component Long-Offset Surface Seismic Survey, PSInSAR, and Kinematic

  9. Exploring Plasma Science Advances from Fusion Findings to Astrophysical

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Achievements | Princeton Plasma Physics Lab Exploring Plasma Science Advances from Fusion Findings to Astrophysical Achievements By John Greenwald December 4, 2012 Tweet Widget Google Plus One Share on Facebook The latest advances in plasma physics were the focus of more than 1,000 scientists from around the world who gathered in Providence, R.I., from Oct. 29 through Nov. 2 for the 54th Annual Meeting of the American Physical Society's Division of Plasma Physics (APS-DPP). Papers, posters

  10. Graph Coarsening for Path Finding in Cybersecurity Graphs

    SciTech Connect (OSTI)

    Hogan, Emilie A.; Johnson, John R.; Halappanavar, Mahantesh

    2013-01-01

    n the pass-the-hash attack, hackers repeatedly steal password hashes and move through a computer network with the goal of reaching a computer with high level administrative privileges. In this paper we apply graph coarsening in network graphs for the purpose of detecting hackers using this attack or assessing the risk level of the network's current state. We repeatedly take graph minors, which preserve the existence of paths in the graph, and take powers of the adjacency matrix to count the paths. This allows us to detect the existence of paths as well as find paths that have high risk of being used by adversaries.

  11. Transportation Energy Futures: Project Overview and Findings (Presentation)

    SciTech Connect (OSTI)

    Not Available

    2013-03-01

    The U.S. Department of Energy-sponsored Transportation Energy Futures (TEF) project examines how combining multiple strategies could reduce both GHG emissions and petroleum use by 80%. The project's primary objective was to help inform domestic decisions about transportation energy strategies, priorities, and investments, with an emphasis on previously underexplored opportunities related to energy efficiency and renewable energy in light-duty vehicles, non-light-duty vehicles, fuels, and transportation demand. This PowerPoint provides an overview of the project and its findings.

  12. Microsoft Word - fact sheet alternatives and key findings 090214.docx

    National Nuclear Security Administration (NNSA)

    Alternatives and Key Findings of the Draft SEIS ALTERNATIVES The Draft SEIS evaluates a range of reactor site and tritium production capacity alternatives. Seven alternatives are analyzed in the Draft SEIS:  Alternative 1 Watts Bar site only (2,500 TPBARs maximum). This is the preferred alternative.  Alternative 2 Sequoyah site only (2,500 TPBARs maximum).  Alternative 3 Both Watts Bar and Sequoyah sites (2,500 TPBARs maximum).  Alternative 4 Watts Bar site only (5,000 TPBARs

  13. Finding Text Information in the Ocean of Electronic Documents

    SciTech Connect (OSTI)

    Medvick, Patricia A.; Calapristi, Augustin J.

    2003-02-05

    Information management in natural resources has become an overwhelming task. A massive amount of electronic documents and data is now available for creating informed decisions. The problem is finding the relevant information to support the decision-making process. Determining gaps in knowledge in order to propose new studies or to determine which proposals to fund for maximum potential is a time-consuming and difficult task. Additionally, available data stores are increasing in complexity; they now may include not only text and numerical data, but also images, sounds, and video recordings. Information visualization specialists at Pacific Northwest National Laboratory (PNNL) have software tools for exploring electronic data stores and for discovering and exploiting relationships within data sets. These provide capabilities for unstructured text explorations, the use of data signatures (a compact format for the essence of a set of scientific data) for visualization (Wong et al 2000), visualizations for multiple query results (Havre et al. 2001), and others (http://www.pnl.gov/infoviz ). We will focus on IN-SPIRE, a MS Windows vision of PNNL’s SPIRE (Spatial Paradigm for Information Retrieval and Exploration). IN-SPIRE was developed to assist information analysts find and discover information in huge masses of text documents.

  14. (Findings of the Costa Rica power sector efficiency study)

    SciTech Connect (OSTI)

    Waddle, D.B.

    1990-10-08

    To present findings of the Costa Rica Power Sector Efficiency Study to the Instituto Costarricense de Electridad, and to the Ministry of Energy, Natural Resources and Mining. To discuss the progress and plans for the Central American Rural Electrification Project with US Agency for International Development (USAID)/Regional Office Central American Program (ROCAP). I traveled to San Jose, Costa Rica to present the findings of the Costa Rica Power Sector Efficiency Study to our counterparts in the utility and the Ministry of Energy. Discussions were held with line level managers at Instituto Costarricensede Electricidad (ICE) and Ministry of Energy Mines and Natural Resources (MIRENEM), as well as a plan of action set for the final stage of the project. Discussions were held for a one day period with both the bilateral Agency for International Development (AID) and the regional AID mission regarding the need for a similar study in Guatemala and matters directly pertaining to the Central American Rural Electrification Study (CARES) project.

  15. Role of a Transcriptional Regulator in Programmed Cell Death and Plant Development

    SciTech Connect (OSTI)

    Julie M. Stone

    2008-09-13

    The long-term goal of this research is to understand the role(s) and molecular mechanisms of programmed cell death (PCD) in the controlling plant growth, development and responses to biotic and abiotic stress. We developed a genetic selection scheme to identify A. thaliana FB1-resistant (fbr) mutants as a way to find genes involved in PCD (Stone et al., 2000; Stone et al., 2005; Khan and Stone, 2008). The disrupted gene in fbr6 (AtSPL14) responsible for the FB1-insensitivity and plant architecture phenotypes encodes a plant-specific SBP DNA-binding domain transcriptional regulator (Stone et al., 2005; Liang et al., 2008). This research plan is designed to fill gaps in the knowledge about the role of SPL14 in plant growth and development. The work is being guided by three objectives aimed at determining the pathways in which SPL14 functions to modulate PCD and/or plant development: (1) determine how SPL14 functions in plant development, (2) identify target genes that are directly regulated by SPL14, and (3) identify SPL14 modifications and interacting proteins. We made significant progress during the funding period. Briefly, some major accomplishments are highlighted below: (1) To identify potential AtSPL14 target genes, we identified a consensus DNA binding site for the AtSPL14 SBP DNA-binding domain using systematic evolution of ligands by exponential selection (SELEX) and site-directed mutagenesis (Liang et al., 2008). This consensus binding site was used to analyze Affymetrix microarray gene expression data obtained from wild-type and fbr6 mutant plants to find possible AtSPL14-regulated genes. These candidate AtSPL14-regulated genes are providing new information on the molecular mechanisms linking plant PCD and plant development through modulation of the 26S proteasome. (2) Transgenic plants expressing epitope-tagged versions of AtSPL14 are being used to confirm the AtSPL14 targets (by ChIP-PCR) and further dissect the molecular interactions (Nazarenus, Liang

  16. Chk2 regulates transcription-independent p53-mediated apoptosis in response to DNA damage

    SciTech Connect (OSTI)

    Chen Chen [Department of Geriatric Research, National Institute for Longevity Sciences, National Center for Geriatrics and Gerontology, Obu, Aichi 474-8522 (Japan); Shimizu, Shigeomi [Department of Post-Genomics Diseases, Osaka University Medical School, Suita, Osaka 565-0871 (Japan); Tsujimoto, Yoshihide [Department of Post-Genomics Diseases, Osaka University Medical School, Suita, Osaka 565-0871 (Japan); Motoyama, Noboru [Department of Geriatric Research, National Institute for Longevity Sciences, National Center for Geriatrics and Gerontology, Obu, Aichi 474-8522 (Japan)]. E-mail: motoyama@nils.go.jp

    2005-07-29

    The tumor suppressor protein p53 plays a central role in the induction of apoptosis in response to genotoxic stress. The protein kinase Chk2 is an important regulator of p53 function in mammalian cells exposed to ionizing radiation (IR). Cells derived from Chk2-deficient mice are resistant to the induction of apoptosis by IR, and this resistance has been thought to be a result of the defective transcriptional activation of p53 target genes. It was recently shown, however, that p53 itself and histone H1.2 translocate to mitochondria and thereby induces apoptosis in a transcription-independent manner in response to IR. We have now examined whether Chk2 also regulates the transcription-independent induction of apoptosis by p53 and histone H1.2. The reduced ability of IR to induce p53 stabilization in Chk2-deficient thymocytes was associated with a marked impairment of p53 and histone H1 translocation to mitochondria. These results suggest that Chk2 regulates the transcription-independent mechanism of p53-mediated apoptosis by inducing stabilization of p53 in response to IR.

  17. Utility of Social Modeling for Proliferation Assessment - Preliminary Findings

    SciTech Connect (OSTI)

    Coles, Garill A.; Gastelum, Zoe N.; Brothers, Alan J.; Thompson, Sandra E.

    2009-07-16

    Often the methodologies for assessing proliferation risk are focused around the inherent vulnerability of nuclear energy systems and associated safeguards. For example an accepted approach involves ways to measure the intrinsic and extrinsic barriers to potential proliferation. This paper describes preliminary investigation into non-traditional use of social and cultural information to improve proliferation assessment and advance the approach to assessing nuclear material diversion. Proliferation resistance assessment, safeguard assessments and related studies typically create technical information about the vulnerability of a nuclear energy system to diversion of nuclear material. The purpose of this research project is to find ways to integrate social information with technical information by explicitly considering the role of culture, groups and/or individuals to factors that impact the possibility of proliferation. When final, this work is expected to describe and demonstrate the utility of social science modeling in proliferation and proliferation risk assessments.

  18. Parallel halo finding in N-body cosmology simulations

    SciTech Connect (OSTI)

    Pfitzner, D.W.; Salmon, J.K.

    1996-12-31

    Cosmological N-body simulations on parallel computers produce large datasets - about five hundred Megabytes at a single output time, or tens of Gigabytes over the course of a simulation. These large datasets require further analysis before they can be compared to astronomical observations. We have implemented two methods for performing halo finding, a key part of the knowledge discovery process, on parallel machines. One of these is a parallel implementation of the friends of friends (FOF) algorithm, widely used in the field of N-body cosmology. The new isodensity (ID) method has been developed to overcome some of the shortcomings of FOR Both have been implemented on a variety of computer systems, and successfully used to extract halos from simulations with up to 256{sup 3} (or about 16.8 million) particles, which axe among the largest N-body cosmology simulations in existence.

  19. Transcriptional responses of Arabidopsis thaliana to chewing and sucking insect herbivores

    SciTech Connect (OSTI)

    Appel, Heidi M.; Fescemyer, Howard; Ehlting, Juergen; Weston, David; Rehrig, Erin; Joshi, Trupti; Xu, Dong; Bohlmann, Joerg; Schultz, Jack

    2014-11-14

    We tested the hypothesis that Arabidopsis can recognize and respond differentially to insect species at the transcriptional level using a genome wide microarray. Transcriptional reprogramming was characterized using co-expression analysis in damaged and undamaged leaves at two times in response to mechanical wounding and four insect species. In all, 2778 (10.6%) of annotated genes on the array were differentially expressed in at least one treatment. Responses differed mainly between aphid and caterpillar and sampling times. Responses to aphids and caterpillars shared only 10% of up-regulated and 8% of down-regulated genes. Responses to two caterpillars shared 21 and 12% of up- and down-regulated genes, whereas responses to the two aphids shared only 7 and 4% of up-regulated and down-regulated genes. Overlap in genes expressed between 6 and 24 h was 3–15%, and depended on the insect species. Responses in attacked and unattacked leaves differed at 6 h but converged by 24 h. Genes responding to the insects are also responsive to many stressors and included primary metabolism. Aphids down-regulated amino acid catabolism; caterpillars stimulated production of amino acids involved in glucosinolate synthesis. Co-expression analysis revealed 17 response networks. Transcription factors were a major portion of differentially expressed genes throughout and responsive genes shared most of the known or postulated binding sites. However, cis-element composition of genes down regulated by the aphid M. persicae was unique, as were those of genes down-regulated by caterpillars. As many as 20 cis-elements were over-represented in one or more treatments, including some from well-characterized classes and others as yet uncharacterized. We suggest that transcriptional changes elicited by wounding and insects are heavily influenced by transcription factors and involve both enrichment of a common set of cis-elements and a unique enrichment of a few cis-elements in responding genes.

  20. Transcriptional responses of Arabidopsis thaliana to chewing and sucking insect herbivores

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Appel, Heidi M.; Fescemyer, Howard; Ehlting, Juergen; Weston, David; Rehrig, Erin; Joshi, Trupti; Xu, Dong; Bohlmann, Joerg; Schultz, Jack

    2014-11-14

    We tested the hypothesis that Arabidopsis can recognize and respond differentially to insect species at the transcriptional level using a genome wide microarray. Transcriptional reprogramming was characterized using co-expression analysis in damaged and undamaged leaves at two times in response to mechanical wounding and four insect species. In all, 2778 (10.6%) of annotated genes on the array were differentially expressed in at least one treatment. Responses differed mainly between aphid and caterpillar and sampling times. Responses to aphids and caterpillars shared only 10% of up-regulated and 8% of down-regulated genes. Responses to two caterpillars shared 21 and 12% of up-more » and down-regulated genes, whereas responses to the two aphids shared only 7 and 4% of up-regulated and down-regulated genes. Overlap in genes expressed between 6 and 24 h was 3–15%, and depended on the insect species. Responses in attacked and unattacked leaves differed at 6 h but converged by 24 h. Genes responding to the insects are also responsive to many stressors and included primary metabolism. Aphids down-regulated amino acid catabolism; caterpillars stimulated production of amino acids involved in glucosinolate synthesis. Co-expression analysis revealed 17 response networks. Transcription factors were a major portion of differentially expressed genes throughout and responsive genes shared most of the known or postulated binding sites. However, cis-element composition of genes down regulated by the aphid M. persicae was unique, as were those of genes down-regulated by caterpillars. As many as 20 cis-elements were over-represented in one or more treatments, including some from well-characterized classes and others as yet uncharacterized. We suggest that transcriptional changes elicited by wounding and insects are heavily influenced by transcription factors and involve both enrichment of a common set of cis-elements and a unique enrichment of a few cis-elements in responding

  1. Histone H4 Lys 20 methyltransferase SET8 promotes androgen receptor-mediated transcription activation in prostate cancer

    SciTech Connect (OSTI)

    Yao, Lushuai; Li, Yanyan; Du, Fengxia; Han, Xiao; Li, Xiaohua; Niu, Yuanjie; Ren, Shancheng; Sun, Yingli

    2014-07-18

    Highlights: • Dihydrotestosterone stimulates H4K20me1 enrichment at the PSA promoter. • SET8 promotes AR-mediated transcription activation. • SET8 interacts with AR and promotes cell proliferation. - Abstract: Histone methylation status in different lysine residues has an important role in transcription regulation. The effect of H4K20 monomethylation (H4K20me1) on androgen receptor (AR)-mediated gene transcription remains unclear. Here we show that AR agonist stimulates the enrichment of H4K20me1 and SET8 at the promoter of AR target gene PSA in an AR dependent manner. Furthermore, SET8 is crucial for the transcription activation of PSA. Co-immunoprecipitation analyses demonstrate that SET8 interacts with AR. Therefore, we conclude that SET8 is involved in AR-mediated transcription activation, possibly through its interaction with AR and H4K20me1 modification.

  2. Low concentrations of bisphenol a suppress thyroid hormone receptor transcription through a nongenomic mechanism

    SciTech Connect (OSTI)

    Sheng, Zhi-Guo; Tang, Yuan; Liu, Yu-Xiang; Yuan, Ye; Zhao, Bao-Quan; Chao, Xi-Juan; Zhu, Ben-Zhan

    2012-02-15

    Bisphenol (BPA) is one of the highest-volume chemicals produced worldwide, and human exposure to BPA is thought to be ubiquitous. Various rodent and in vitro studies have shown that thyroid hormone (TH) function can be impaired by BPA. However, it is still unknown if low concentrations of BPA can suppress the thyroid hormone receptor (TR) transcription. The present study aims to investigate the possible suppressing effects of low concentrations of BPA on TR transcription and the involved mechanism(s) in CV-1 cells derived from cercopithecus aethiops monkey kidneys. Using gene reporter assays, BPA at concentrations as low as 10{sup ?9} M suppresses TR or steroid receptor coactivator-1(SRC-1)-enhanced TR transcription, but not reducing TR/SRC-1 interaction in mammalian two-hybrid and glutathione S-transferase pull-down studies. It has been further shown that both nuclear receptor co-repressor (N-CoR) and silencing mediator for retinoid and thyroid hormone receptors (SMRT) are recruited to the TR-?1 by BPA in the presence of physiologic concentrations of T3 or T4. However, the overexpression of ?3 integrin or c-Src significantly reduces BPA-induced recruitment of N-CoR/SMRT to TR or suppression of TR transcription. Furthermore, BPA inhibits the T3/T4-mediated interassociation of the ?3 integrin/c-Src/MAPK/TR-?1 pathways by the co-immunoprecipitation. These results indicate that low concentrations of BPA suppress the TR transcription by disrupting physiologic concentrations of T3/T4-mediated ?3 integrin/c-Src/MAPK/TR-?1 pathways, followed by recruiting N-CoR/SMRT to TR-?1, providing a novel insight regarding the TH disruption effects of low concentration BPA. -- Highlights: ? Environmentally relevant concentrations of BPA suppress TR transcription. ? BPA recruits the N-CoR/SMRT to TR under the physiologic concentrations of T3/T4. ? BPA disrupts T3/T4-mediated ?3 integrin/c-Src/MAPK/TR-?1 pathways.

  3. Energy economists unite. [International Association of Energy Economists findings

    SciTech Connect (OSTI)

    Not Available

    1983-08-10

    Three years after the Arab Oil Embargo, the International Association of Energy Economists (IAEE) was organized. A non-profit organization operated through a council of 15 elected and appointed members, IAEE has already attracted some 1400 individual members from around the world as well as numerous affiliates among national energy organizations. In this issue, Energy Detente reports various IAEE findings, including: (1) a possible 1% growth during 1983 in total energy demand in capitalist countries to be satisfied by coal, nuclear, gas, and hydro supplies, not petroleum; petroleum is expected to figure in the expected 4% growth in energy demand during 1984; (2) usable commercial inventory of petroleum, having risen to 27 days of forward demand by the second quarter of 1981, now sits at 10 days; (3) the current cushion between oil supply and demand, now 15-million barrels per day (b/d) on the side of surplus production capacity during the first quarter of 1983, is exerting downward pressure on prices; and (4) possibly only half the effect of conservation provoked by the second oil-price shock has yet been manifested. If prices fall below US $25 bbl, the second half might be deferred indefinitely, and some enhanced-recovery and frontier-recovery projects would become uneconomic. Also, with OPEC prices holding through the summer of 1983, there is a good chance prices could stabilize for the next several years. This issue presents the Energy Detente fuel price/tax series and the principal industrial fuel prices for August 1983 for countries of the Eastern Hemisphere.

  4. Molecular genetic analysis of activation-tagged transcription factors thought to be involved in photomorphogenesis

    SciTech Connect (OSTI)

    Neff, Michael M.

    2011-06-23

    This is a final report for Department of Energy Grant No. DE-FG02-08ER15927 entitled “Molecular Genetic Analysis of Activation-Tagged Transcription Factors Thought to be Involved in Photomorphogenesis”. Based on our preliminary photobiological and genetic analysis of the sob1-D mutant, we hypothesized that OBP3 is a transcription factor involved in both phytochrome and cryptochrome-mediated signal transduction. In addition, we hypothesized that OBP3 is involved in auxin signaling and root development. Based on our preliminary photobiological and genetic analysis of the sob2-D mutant, we also hypothesized that a related gene, LEP, is involved in hormone signaling and seedling development.

  5. Fungal-specific transcription factor AbPf2 activates pathogenicity in Alternaria brassicicola

    SciTech Connect (OSTI)

    Cho, Yangrae; Ohm, Robin A.; Grigoriev, Igor V.; Srivastava, Akhil

    2012-12-03

    Alternaria brassicicola is a successful saprophyte and necrotrophic plant pathogen. To identify molecular determinants of pathogenicity, we created non-pathogenic mutants of a transcription factor-encoding gene, AbPf2. The frequency and timing of germination and appressorium formation on host plants were similar between the non-pathogenic abpf2 mutants and wild-type A. brassicicola. The mutants were also similar in vitro to wild-type A. brassicicola in terms of vegetative growth, conidium production, and responses to a phytoalexin, reactive oxygen species and osmolites. The hyphae of the mutants grew slowly but did not cause disease symptoms on the surface of host plants. Transcripts of the AbPf2 gene increased exponentially soon after wild-type conidia contacted their host plants . A small amount of AbPf2 protein, as monitored using GFP fusions, was present in young, mature conidia. The protein level decreased during saprophytic growth, but increased and was located primarily in fungal nuclei during pathogenesis. Levels of the proteins and transcripts sharply decreased following colonization of host tissues beyond the initial infection site. When expression of the transcription factor was induced in the wild-type during early pathogenesis, 106 fungal genes were also induced in the wild-type but not in the abpf2 mutants. Notably, 33 of the 106 genes encoded secreted proteins, including eight putative effector proteins. Plants inoculated with abpf2 mutants expressed higher levels of genes associated with photosynthesis, the pentose phosphate pathway and primary metabolism, but lower levels of defense-related genes. Our results suggest that AbPf2 is an important regulator of pathogenesis, but does not affect other cellular processes in A. brassicicola.

  6. Transcript of May 4 Webinar: Understanding the Energy Policy and Regulatory

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Environment | Department of Energy 4 Webinar: Understanding the Energy Policy and Regulatory Environment Transcript of May 4 Webinar: Understanding the Energy Policy and Regulatory Environment Good morning, or good afternoon, wherever you may be and welcome to the fifth webinar of the 2016 DOE's Tribal Renewable Energy Webinar Series, "Understanding the Energy Policy and Regulatory Environment." I'm Randy Manion, today's webinar chair and manager of Western Area Power

  7. Transcript abundance on its own cannot be used to infer fluxes in central metabolism

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Schwender, Jorg; Konig, Christina; Klapperstuck, Matthias; Heinzel, Nicolas; Munz, Eberhard; Hebbelmann, Inga; Hay, Jordan O.; Denolf, Peter; De Bodt, Stefanie; Redestig, Henning; et al

    2014-11-28

    An attempt has been made to define the extent to which metabolic flux in central plant metabolism is reflected by changes in the transcriptome and metabolome, based on an analysis of in vitro cultured immature embryos of two oilseed rape (Brassica napus) accessions which contrast for seed lipid accumulation. Metabolic flux analysis (MFA) was used to constrain a flux balance metabolic model which included 671 biochemical and transport reactions within the central metabolism. This highly confident flux information was eventually used for comparative analysis of flux vs. transcript (metabolite). Metabolite profiling succeeded in identifying 79 intermediates within the central metabolism,more » some of which differed quantitatively between the two accessions and displayed a significant shift corresponding to flux. An RNA-Seq based transcriptome analysis revealed a large number of genes which were differentially transcribed in the two accessions, including some enzymes/proteins active in major metabolic pathways. With a few exceptions, differential activity in the major pathways (glycolysis, TCA cycle, amino acid, and fatty acid synthesis) was not reflected in contrasting abundances of the relevant transcripts. The conclusion was that transcript abundance on its own cannot be used to infer metabolic activity/fluxes in central plant metabolism. Lastly, this limitation needs to be borne in mind in evaluating transcriptome data and designing metabolic engineering experiments.« less

  8. Transcript abundance on its own cannot be used to infer fluxes in central metabolism

    SciTech Connect (OSTI)

    Schwender, Jorg; Konig, Christina; Klapperstuck, Matthias; Heinzel, Nicolas; Munz, Eberhard; Hebbelmann, Inga; Hay, Jordan O.; Denolf, Peter; De Bodt, Stefanie; Redestig, Henning; Caestecker, Evelyne; Jakob, Peter M.; Borisjuk, Ljudmilla; Rolletschek, Hardy

    2014-11-28

    An attempt has been made to define the extent to which metabolic flux in central plant metabolism is reflected by changes in the transcriptome and metabolome, based on an analysis of in vitro cultured immature embryos of two oilseed rape (Brassica napus) accessions which contrast for seed lipid accumulation. Metabolic flux analysis (MFA) was used to constrain a flux balance metabolic model which included 671 biochemical and transport reactions within the central metabolism. This highly confident flux information was eventually used for comparative analysis of flux vs. transcript (metabolite). Metabolite profiling succeeded in identifying 79 intermediates within the central metabolism, some of which differed quantitatively between the two accessions and displayed a significant shift corresponding to flux. An RNA-Seq based transcriptome analysis revealed a large number of genes which were differentially transcribed in the two accessions, including some enzymes/proteins active in major metabolic pathways. With a few exceptions, differential activity in the major pathways (glycolysis, TCA cycle, amino acid, and fatty acid synthesis) was not reflected in contrasting abundances of the relevant transcripts. The conclusion was that transcript abundance on its own cannot be used to infer metabolic activity/fluxes in central plant metabolism. Lastly, this limitation needs to be borne in mind in evaluating transcriptome data and designing metabolic engineering experiments.

  9. DNA Recognition by a σ54 Transcriptional Activator from Aquifex aeolicus

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Vidangos, Natasha K.; Heideker, Johanna; Lyubimov, Artem; Lamers, Meindert; Huo, Yixin; Pelton, Jeffrey G.; Ton, Jimmy; Gralla, Jay; Berger, James; Wemmer, David E.

    2014-08-23

    Transcription initiation by bacterial σ54-polymerase requires the action of a transcriptional activator protein. Activators bind sequence-specifically upstream of the transcription initiation site via a DNA-binding domain. The structurally characterized DNA-binding domains from activators all belong to the Factor for Inversion Stimulation (Fis) family of helix-turn-helix DNA-binding proteins. We report here structures of the free and DNA-bound forms of the DNA-binding domain of NtrC4 (4DBD) from Aquifex aeolicus, a member of the NtrC family of σ54 activators. Two NtrC4 binding sites were identified upstream (-145 and -85 base pairs) from the start of the lpxC gene, which is responsible for themore » first committed step in Lipid A biosynthesis. This is the first experimental evidence for σ54 regulation in lpxC expression. 4DBD was crystallized both without DNA and in complex with the -145 binding site. The structures, together with biochemical data, indicate that NtrC4 binds to DNA in a manner that is similar to that of its close homologue, Fis. Ultimately, the greater sequence specificity for the binding of 4DBD relative to Fis seems to arise from a larger number of base specific contacts contributing to affinity than for Fis.« less

  10. Double-stranded RNA transcribed from vector-based oligodeoxynucleotide acts as transcription factor decoy

    SciTech Connect (OSTI)

    Xiao, Xiao; Gang, Yi; Wang, Honghong; Wang, Jiayin; Zhao, Lina; Xu, Li; Liu, Zhiguo

    2015-02-06

    Highlights: • A shRNA vector based transcription factor decoy, VB-ODN, was designed. • VB-ODN for NF-κB inhibited cell viability in HEK293 cells. • VB-ODN inhibited expression of downstream genes of target transcription factors. • VB-ODN may enhance nuclear entry ratio for its feasibility of virus production. - Abstract: In this study, we designed a short hairpin RNA vector-based oligodeoxynucleotide (VB-ODN) carrying transcription factor (TF) consensus sequence which could function as a decoy to block TF activity. Specifically, VB-ODN for Nuclear factor-κB (NF-κB) could inhibit cell viability and decrease downstream gene expression in HEK293 cells without affecting expression of NF-κB itself. The specific binding between VB-ODN produced double-stranded RNA and NF-κB was evidenced by electrophoretic mobility shift assay. Moreover, similar VB-ODNs designed for three other TFs also inhibit their downstream gene expression but not that of themselves. Our study provides a new design of decoy for blocking TF activity.

  11. Oak Ridge Finds Ways to Remove K-25 Faster, Cheaper | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finds Ways to Remove K-25 Faster, Cheaper Oak Ridge Finds Ways to Remove K-25 Faster, Cheaper February 1, 2012 - 12:00pm Addthis Oak Ridge Finds Ways to Remove K-25 Faster, Cheaper ...

  12. EA-1761: Finding of No Significant Impact and Floodplain Statement of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Findings | Department of Energy 61: Finding of No Significant Impact and Floodplain Statement of Findings EA-1761: Finding of No Significant Impact and Floodplain Statement of Findings Clemson University Wind Turbine Drivetrain Test Facility North Charleston, South Carolina The Department of Energy is proposing to provide federal funding to Clemson University to design, permit, and construct a Wind Turbine Drivetrain Test Facility. Finding of No Significant Impact and Floodplain Statement of

  13. CITED2 modulates estrogen receptor transcriptional activity in breast cancer cells

    SciTech Connect (OSTI)

    Lau, Wen Min; Doucet, Michele; Huang, David; Weber, Kristy L.; Kominsky, Scott L.

    2013-07-26

    Highlights: The effects of elevated CITED2 on ER function in breast cancer cells are examined. CITED2 enhances cell growth in the absence of estrogen and presence of tamoxifen. CITED2 functions as a transcriptional co-activator of ER in breast cancer cells. -- Abstract: Cbp/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain 2 (CITED2) is a member of the CITED family of non-DNA binding transcriptional co-activators of the p300/CBP-mediated transcription complex. Previously, we identified CITED2 as being overexpressed in human breast tumors relative to normal mammary epithelium. Upon further investigation within the estrogen receptor (ER)-positive subset of these breast tumor samples, we found that CITED2 mRNA expression was elevated in those associated with poor survival. In light of this observation, we investigated the effect of elevated CITED2 levels on ER function. While ectopic overexpression of CITED2 in three ER-positive breast cancer cell lines (MCF-7, T47D, and CAMA-1) did not alter cell proliferation in complete media, growth was markedly enhanced in the absence of exogenous estrogen. Correspondingly, cells overexpressing CITED2 demonstrated reduced sensitivity to the growth inhibitory effects of the selective estrogen receptor modulator, 4-hydroxytamoxifen. Subsequent studies revealed that basal ER transcriptional activity was elevated in CITED2-overexpressing cells and was further increased upon the addition of estrogen. Similarly, basal and estrogen-induced expression of the ER-regulated genes trefoil factor 1 (TFF1) and progesterone receptor (PGR) was higher in cells overexpressing CITED2. Concordant with this observation, ChIP analysis revealed higher basal levels of CITED2 localized to the TFF-1 and PGR promoters in cells with ectopic overexpression of CITED2, and these levels were elevated further in response to estrogen stimulation. Taken together, these data indicate that CITED2 functions as a transcriptional co

  14. Sex-related differences in murine hepatic transcriptional and proteomic responses to TCDD

    SciTech Connect (OSTI)

    Prokopec, Stephenie D.; Watson, John D.; Lee, Jamie; Pohjanvirta, Raimo; Boutros, Paul C.

    2015-04-15

    2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is an environmental contaminant that produces myriad toxicities in most mammals. In rodents alone, there is a huge divergence in the toxicological response across species, as well as among different strains within a species. But there are also significant differences between males and females animals of a single strain. These differences are inconsistent across model systems: the severity of toxicity is greater in female rats than males, while male mice and guinea pigs are more sensitive than females. Because the specific events that underlie this difference remain unclear, we characterized the hepatic transcriptional response of adult male and female C57BL/6 mice to 500 μg/kg TCDD at multiple time-points. The transcriptional profile diverged significantly between the sexes. Female mice demonstrated a large number of altered transcripts as early as 6 h following treatment, suggesting a large primary response. Conversely, male animals showed the greatest TCDD-mediated response 144 h following exposure, potentially implicating significant secondary responses. Nr1i3 was statistically significantly induced at all time-points in the sensitive male animals. This mRNA encodes the constitutive androstane receptor (CAR), a transcription factor involved in the regulation of xenobiotic metabolism, lipid metabolism, cell cycle and apoptosis. Surprisingly though, changes at the protein level (aside from the positive control, CYP1A1) were modest, with only FMO3 showing clear induction, and no genes with sex-differences. Thus, while male and female mice show transcriptional differences in their response to TCDD, their association with TCDD-induced toxicities remains unclear. - Highlights: • Differences exist between the toxicity phenotypes to TCDD in male and female mice. • TCDD-mediated transcriptomic differences were identified between the sexes. • Resistant female mice displayed a large, early-onset, transcriptomic response.

  15. Identification and overexpression of a knotted1-like transcription factor in switchgrass (Panicum virgatum L.) for lignocellulosic feedstock improvement

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Wuddineh, Wegi A.; Mazarei, Mitra; Zhang, Ji -Yi; Turner, Geoffrey B.; Sykes, Robert W.; Decker, Stephen R.; Davis, Mark F.; Udvardi, Michael K.; C. Neal Stewart, Jr.

    2016-04-28

    High biomass production and wide adaptation has made switchgrass (Panicum virgatum L.) an important candidate lignocellulosic bioenergy crop. One major limitation of this and other lignocellulosic feedstocks is the recalcitrance of complex carbohydrates to hydrolysis for conversion to biofuels. Lignin is the major contributor to recalcitrance as it limits the accessibility of cell wall carbohydrates to enzymatic breakdown into fermentable sugars. Therefore, genetic manipulation of the lignin biosynthesis pathway is one strategy to reduce recalcitrance. Here, we identified a switchgrass Knotted1 transcription factor, PvKN1, with the aim of genetically engineering switchgrass for reduced biomass recalcitrance for biofuel production. Gene expressionmore » of the endogenous PvKN1 gene was observed to be highest in young inflorescences and stems. Ectopic overexpression of PvKN1 in switchgrass altered growth, especially in early developmental stages. Transgenic lines had reduced expression of most lignin biosynthetic genes accompanied by a reduction in lignin content suggesting the involvement of PvKN1 in the broad regulation of the lignin biosynthesis pathway. Moreover, the reduced expression of the Gibberellin 20-oxidase (GA20ox) gene in tandem with the increased expression of Gibberellin 2-oxidase (GA2ox) genes in transgenic PvKN1 lines suggest that PvKN1 may exert regulatory effects via modulation of GA signaling. Furthermore, overexpression of PvKN1 altered the expression of cellulose and hemicellulose biosynthetic genes and increased sugar release efficiency in transgenic lines. Our findings demonstrated that switchgrass PvKN1 is a putative ortholog of maize KN1 that is linked to plant lignification and cell wall and development traits as a major regulatory gene. Therefore, targeted overexpression of PvKN1 in bioenergy feedstocks may provide one feasible strategy for reducing biomass recalcitrance and simultaneously improving plant growth characteristics.« less

  16. EM Finds Success with Fixed-Priced Hybrid Contract Approach Benefittin...

    Office of Environmental Management (EM)

    Finds Success with Fixed-Priced Hybrid Contract Approach Benefitting Taxpayers EM Finds Success with Fixed-Priced Hybrid Contract Approach Benefitting Taxpayers January 29, 2014 - ...

  17. EA-1774-S1: Final Environmental Assessment and Finding of No...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    4-S1: Final Environmental Assessment and Finding of No Significant Impact EA-1774-S1: Final Environmental Assessment and Finding of No Significant Impact Energy Conservation ...

  18. The oncoprotein HBXIP upregulates PDGFB via activating transcription factor Sp1 to promote the proliferation of breast cancer cells

    SciTech Connect (OSTI)

    Zhang, Yingyi; Zhao, Yu; Li, Leilei; Shen, Yu; Cai, Xiaoli; Zhang, Xiaodong; Ye, Lihong

    2013-05-03

    Highlights: HBXIP is able to upregulate the expression of PDGFB in breast cancer cells. HBXIP serves as a coactivator of activating transcription factor Sp1. HBXIP stimulates the PDGFB promoter via activating transcription factor Sp1. HBXIP promotes the proliferation of breast cancer cell via upregulating PDGFB. -- Abstract: We have reported that the oncoprotein hepatitis B virus X-interacting protein (HBXIP) acts as a novel transcriptional coactivator to promote proliferation and migration of breast cancer cells. Previously, we showed that HBXIP was able to activate nuclear factor-?B (NF-?B) in breast cancer cells. As an oncogene, the platelet-derived growth factor beta polypeptide (PDGFB) plays crucial roles in carcinogenesis. In the present study, we found that both HBXIP and PDGFB were highly expressed in breast cancer cell lines. Interestingly, HBXIP was able to increase transcriptional activity of NF-?B through PDGFB, suggesting that HBXIP is associated with PDGFB in the cells. Moreover, HBXIP was able to upregulate PDGFB at the levels of mRNA, protein and promoter in the cells. Then, we identified that HBXIP stimulated the promoter of PDGFB through activating transcription factor Sp1. In function, HBXIP enhanced the proliferation of breast cancer cells through PDGFB in vitro. Thus, we conclude that HBXIP upregulates PDGFB via activating transcription factor Sp1 to promote proliferation of breast cancer cells.

  19. Light-up and FRET aptamer reporters; evaluating their applications for imaging transcription in eukaryotic cells

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Ilgu, Muslum; Ray, Judhajeet; Bendickson, Lee; Wang, Tianjiao; Geraskin, Ivan M.; Kraus, George A.; Nilsen-Hamilton, Marit

    2015-12-17

    The regulation of RNA transcription is central to cellular function. Changes in gene expression drive differentiation and cellular responses to events such as injury. RNA trafficking can also have a large impact on protein expression and its localization. Thus, the ability to image RNA transcription and trafficking in real time and in living cells is a worthwhile goal that has been difficult to achieve. The availability of “light-up” aptamers that cause an increase in fluorescence of their ligands when bound by the aptamer have shown promise for reporting on RNA production and localization in vivo. Here we have investigated twomore » light-up aptamers (the malachite green aptamer and the Spinach aptamers) for their suitabilities as reporters of RNA expression in vivo using two eukaryotic cell types, yeast and mammalian. Our analysis focused on the aptamer ligands, their contributions to background noise, and the impact of tandem aptamer strings on signal strength and ligand affinity. Whereas the background fluorescence is very low in vitro, this is not always true for cell imaging. Our results suggest the need for caution in using light-up aptamers as reporters for imaging RNA. In particular, images should be collected and analyzed by operators blinded to the sample identities. The appropriate control condition of ligand with the cells in the absence of aptamer expression must be included in each experiment. This control condition establishes that the specific interaction of ligand with aptamer, rather than nonspecific interactions with unknown cell elements, is responsible for the observed fluorescent signals. As a result, high background signals due to nonspecific interactions of aptamer ligands with cell components can be minimized by using IMAGEtags (Intracellular Multiaptamer GEnetic tags), which signal by FRET and are promising RNA reporters for imaging transcription.« less

  20. Human mitochondrial transcription factor A functions in both nuclei and mitochondria and regulates cancer cell growth

    SciTech Connect (OSTI)

    Han, Bin; Izumi, Hiroto; Yasuniwa, Yoshihiro; Akiyama, Masaki; Yamaguchi, Takahiro; Fujimoto, Naohiro; Matsumoto, Tetsuro; Wu, Bin; Tanimoto, Akihide; Sasaguri, Yasuyuki; Kohno, Kimitoshi

    2011-04-29

    Highlights: {yields} Mitochondrial transcription factor A (mtTFA) localizes in nuclei and binds tightly to the nuclear chromatin. {yields} mtTFA contains two putative nuclear localization signals (NLS) in the HMG-boxes. {yields} Overexpression of mtTFA enhances the growth of cancer cells, whereas downregulation of mtTFA inhibits their growth by regulating mtTFA target genes, such as baculoviral IAP repeat-containing 5 (BIRC5; also known as survivin). {yields} Knockdown of mtTFA expression induces p21-dependent G1 cell cycle arrest. -- Abstract: Mitochondrial transcription factor A (mtTFA) is one of the high mobility group protein family and is required for both transcription from and maintenance of mitochondrial genomes. However, the roles of mtTFA have not been extensively studied in cancer cells. Here, we firstly reported the nuclear localization of mtTFA. The proportion of nuclear-localized mtTFA varied among different cancer cells. Some mtTFA binds tightly to the nuclear chromatin. DNA microarray and chromatin immunoprecipitation assays showed that mtTFA can regulate the expression of nuclear genes. Overexpression of mtTFA enhanced the growth of cancer cell lines, whereas downregulation of mtTFA inhibited their growth by regulating mtTFA target genes, such as baculoviral IAP repeat-containing 5 (BIRC5; also known as survivin). Knockdown of mtTFA expression induced p21-dependent G1 cell cycle arrest. These results imply that mtTFA functions in both nuclei and mitochondria to promote cell growth.

  1. Light-up and FRET aptamer reporters; evaluating their applications for imaging transcription in eukaryotic cells

    SciTech Connect (OSTI)

    Ilgu, Muslum; Ray, Judhajeet; Bendickson, Lee; Wang, Tianjiao; Geraskin, Ivan M.; Kraus, George A.; Nilsen-Hamilton, Marit

    2015-12-17

    The regulation of RNA transcription is central to cellular function. Changes in gene expression drive differentiation and cellular responses to events such as injury. RNA trafficking can also have a large impact on protein expression and its localization. Thus, the ability to image RNA transcription and trafficking in real time and in living cells is a worthwhile goal that has been difficult to achieve. The availability of “light-up” aptamers that cause an increase in fluorescence of their ligands when bound by the aptamer have shown promise for reporting on RNA production and localization in vivo. Here we have investigated two light-up aptamers (the malachite green aptamer and the Spinach aptamers) for their suitabilities as reporters of RNA expression in vivo using two eukaryotic cell types, yeast and mammalian. Our analysis focused on the aptamer ligands, their contributions to background noise, and the impact of tandem aptamer strings on signal strength and ligand affinity. Whereas the background fluorescence is very low in vitro, this is not always true for cell imaging. Our results suggest the need for caution in using light-up aptamers as reporters for imaging RNA. In particular, images should be collected and analyzed by operators blinded to the sample identities. The appropriate control condition of ligand with the cells in the absence of aptamer expression must be included in each experiment. This control condition establishes that the specific interaction of ligand with aptamer, rather than nonspecific interactions with unknown cell elements, is responsible for the observed fluorescent signals. As a result, high background signals due to nonspecific interactions of aptamer ligands with cell components can be minimized by using IMAGEtags (Intracellular Multiaptamer GEnetic tags), which signal by FRET and are promising RNA reporters for imaging transcription.

  2. Metabolism and expression of RNA polymerase II transcripts in Influenza virus-infected cells

    SciTech Connect (OSTI)

    Katze, M.G.; Krug, R.M.

    1984-10-01

    Influenza virus infection has adverse effects on the metabolism of two representative RNA polymerase II transcripts in chicken embryo fibroblasts, those coding for BETA-actin and for avian leukosis virus (ALV) proteins. Proviral ALV DNA was integrated into host cell DNA by prior infection with ALV. By S1 endonuclease assay, it was confirmed that nuclear ALV transcripts disappeared very early after infection, already decreasing ca. 80% by 1 h postinfection. A plausible explanation for this nuclear degradation is that the viral cap-dependent endonuclease in the nucleas cleaves the 5' ends of new polymerase II transcripts, rendering the resulting decapped RNAs susceptible to hydrolysis by cellular nucleases. Similar stability of cytoplasmic host cell mRNAs was observed in infected HeLa cells, in which the levels of actin mRNA and two HeLa cell mRNAs (pHe 7 and pHe 28) remained at undiminished levels for 3 h of infection and decreased only slightly by 4.5 h postinfection. The cytoplamic actin and pHe 7 mRNAs isolated from infected HeLa cells were shown to be translated in reticulocyte extracts in biro, indicating that host mRNAs were not inactivated by a virus-induced modification. Despite the continued presence of high levels of functional host cell mRNAs, host cell protein synthesis was effectively shut off by about 3 h postinfection in both chicken embryo fibroblasts and HeLa cells. These results are consistent with the establishment of an influenza virus-specific translational system that selectively translates viral and not host mRNAs.

  3. Transcription Factor Amr1 Induces Melanin Biosynthesis and Suppresses Virulence in Alternaria brassicicola

    SciTech Connect (OSTI)

    Cho, Yangrae; Srivastava, Akhil; Ohm, Robin A.; Lawrence, Christopher B.; Wang, Koon-Hui; Grigoriev, Igor V.; Marahatta, Sharadchandra P.

    2012-05-01

    Alternaria brassicicola is a successful saprophyte and necrotrophic plant pathogen. Several A. brassicicola genes have been characterized as affecting pathogenesis of Brassica species. To study regulatory mechanisms of pathogenesis, we mined 421 genes in silico encoding putative transcription factors in a machine-annotated, draft genome sequence of A. brassicicola. In this study, targeted gene disruption mutants for 117 of the transcription factor genes were produced and screened. Three of these genes were associated with pathogenesis. Disruption mutants of one gene (AbPacC) were nonpathogenic and another gene (AbVf8) caused lesions less than half the diameter of wild-type lesions. Unexpectedly, mutants of the third gene, Amr1, caused lesions with a two-fold larger diameter than the wild type and complementation mutants. Amr1 is a homolog of Cmr1, a transcription factor that regulates melanin biosynthesis in several fungi. We created gene deletion mutants of ?amr1 and characterized their phenotypes. The ?amr1 mutants used pectin as a carbon source more efficiently than the wild type, were melanin-deficient, and more sensitive to UV light and glucanase digestion. The AMR1 protein was localized in the nuclei of hyphae and in highly melanized conidia during the late stage of plant pathogenesis. RNA-seq analysis revealed that three genes in the melanin biosynthesis pathway, along with the deleted Amr1 gene, were expressed at low levels in the mutants. In contrast, many hydrolytic enzyme-coding genes were expressed at higher levels in the mutants than in the wild type during pathogenesis. The results of this study suggested that a gene important for survival in nature negatively affected virulence, probably by a less efficient use of plant cell-wall materials. We speculate that the functions of the Amr1 gene are important to the success of A. brassicicola as a competitive saprophyte and plant parasite.

  4. Inhibition of hepatitis B virus (HBV) by LNA-mediated nuclear interference with HBV DNA transcription

    SciTech Connect (OSTI)

    Sun, Zhen; Department of Biochemistry and Molecular Biology, Program in Molecular Cell Biology, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058 ; Xiang, Wenqing; Guo, Yajuan; Chen, Zhi; Liu, Wei; Lu, Daru

    2011-06-10

    Highlights: {yields} LNA-modified oligonucleotides can pass through the plasma membrane of cultured cells even without using transfection machinery. {yields} LNA-modified oligonucleotides passed efficiently across the cell membrane, and lipid-coating facilitated translocation from the cytoplasm to the nucleus. {yields} LNA-oligonucleotide designed to target nuclear HBV DNA efficiently suppresses HBV replication and transcription in cultured hepatic cells. -- Abstract: Silencing target genes with small regulatory RNAs is widely used to investigate gene function and therapeutic drug development. Recently, triplex-based approaches have provided another attractive means to achieve targeted gene regulation and gene manipulation at the molecular and cellular levels. Nuclear entry of oligonucleotides and enhancement of their affinity to the DNA targets are key points of such approaches. In this study, we developed lipid-based transport of a locked-nucleic-acid (LNA)-modified oligonucleotide for hepatitis B virus (HBV) DNA interference in human hepatocytes expressing HBV genomic DNA. In these cells, the LNA-modified oligonucleotides passed efficiently across the cell membrane, and lipid-coating facilitated translocation from the cytoplasm to the nucleus. The oligonucleotide specifically targeting HBV DNA clearly interfered with HBV DNA transcription as shown by a block in pregenomic RNA (pgRNA) production. The HBV DNA-targeted oligonucleotide suppressed HBV DNA replication and HBV protein production more efficiently than small interfering RNAs directed to the pgRNA. These results demonstrate that fusion with lipid can carry LNA-modified oligonucleotides to the nucleus where they regulate gene expression. Interfering with HBV DNA transcription by LNA-modified oligonucleotides has strong potential as a new strategy for HBV inhibition.

  5. Structurally distinct polycyclic aromatic hydrocarbons induce differential transcriptional responses in developing zebrafish

    SciTech Connect (OSTI)

    Goodale, Britton C.; Tilton, Susan C.; Corvi, Margaret M.; Wilson, Glenn R.; Janszen, Derek B.; Anderson, Kim A.; Waters, Katrina M.; Tanguay, Robert L.

    2013-11-01

    Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous in the environment as components of fossil fuels and by-products of combustion. These multi-ring chemicals differentially activate the aryl hydrocarbon receptor (AHR) in a structurally dependent manner, and induce toxicity via both AHR-dependent and -independent mechanisms. PAH exposure is known to induce developmental malformations in zebrafish embryos, and recent studies have shown cardiac toxicity induced by compounds with low AHR affinity. Unraveling the potentially diverse molecular mechanisms of PAH toxicity is essential for understanding the hazard posed by complex PAH mixtures present in the environment. We analyzed transcriptional responses to PAH exposure in zebrafish embryos exposed to benz(a)anthracene (BAA), dibenzothiophene (DBT) and pyrene (PYR) at concentrations that induced developmental malformations by 120 h post-fertilization (hpf). Whole genome microarray analysis of mRNA expression at 24 and 48 hpf identified genes that were differentially regulated over time and in response to the three PAH structures. PAH body burdens were analyzed at both time points using GCMS, and demonstrated differences in PAH uptake into the embryos. This was important for discerning dose-related differences from those that represented unique molecular mechanisms. While BAA misregulated the least number of transcripts, it caused strong induction of cyp1a and other genes known to be downstream of the AHR, which were not induced by the other two PAHs. Analysis of functional roles of misregulated genes and their predicted regulatory transcription factors also distinguished the BAA response from regulatory networks disrupted by DBT and PYR exposure. These results indicate that systems approaches can be used to classify the toxicity of PAHs based on the networks perturbed following exposure, and may provide a path for unraveling the toxicity of complex PAH mixtures. - Highlights: Defined global mRNA expression

  6. DOE Advanced Manufacturing Office EnPI V3.0 Webinar Transcription

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Advanced Manufacturing Office EnPI V3.0 Webinar Transcription December 11, 2012 Lindsay Southerland: Good Afternoon. My name is Lindsay Southerland and I'm with BCS, Incorporated. It is my pleasure to welcome you to this afternoon's webcast, which is sponsored by the U.S. Department of Energy's Advanced Manufacturing Office, or AMO for short. Today's presenters are Andre de Fontaine of AMO and Ashly Spevacek of the Project Performance Corporation. Ashly and Andre will provide an overview and

  7. Microsoft Word - Consolidated Final Complete Transcript CBS Meeting Boston_Final v1

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Hyatt Regency Boston One Avenue De Lafayette Boston, MA 02111 June 2, 2016 FULL TRANSCRIPT Mr. Jim Hamilton. Good afternoon. Welcome to Boston, and to the fifth in a series of public meetings the Department is hosting on its Consent-Based Siting Program. Thank you all for being here today. I'm especially happy to welcome you for two reasons. One is, I live in Boston, so, welcome to my hometown. But more importantly, we've got an excellent panel here in front of you here today, and I'm looking

  8. Microsoft Word - Consolidated Full Transcript of DOE CBS Meeting Minneapolis 21 JUL 16_Final

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    CONSENT-BASED SITING PUBLIC MEETING Hilton Minneapolis 1001 Marquette Avenue Minneapolis, MN 55403 July 21, 2016 FULL TRANSCRIPT Mr. Jim Hamilton. Good afternoon. And for those joining us in later time zones via webinar, good evening. Welcome to Minneapolis and to the eighth in a series of public meetings the Department is hosting on its Consent-Based Siting Program. Thank you all very much for being here today. My name is Jim Hamilton; I'm an advisor to the Department as part of their

  9. Microsoft Word - Final Consolitated Full Transcript Boise CBS Meeting_Final

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    P a g e CONSENT-BASED SITING PUBLIC MEETING Boise Centre 850 West Front Street Boise, ID 83702 July 14, 2016 FULL TRANSCRIPT Mr. Jim Hamilton. Good afternoon, and for those of you joining us in a later time zone, through the webinar, good evening. Welcome to Boise, and to the seventh in a series of public meetings the Department is hosting on its Consent-Based Siting Program. Thank you all for being here today. My name is Jim Hamilton; I'm an advisor to the Department as part of its

  10. Microsoft Word - Transcript_Pre-2009 Congestion Study_San Francisco2.doc

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    /11/2008 San Francisco, CA Page 1 U.S. Department of Energy Pre-Congestion Study Regional Workshops for the 2009 National Electric Congestion Study San Francisco, CA June 11, 2008 9:00 a.m.-12:30 p.m. Transcript David Meyer: Well, good morning again, ladies and gentlemen, and thank you for coming to DOE's regional workshop on transmission congestion, and welcome also to those who have logged onto the webcast. Because of the, this program is being webcast, we will adhere strictly to our schedule

  11. ECRbase: Database of Evolutionary Conserved Regions, Promoters, and Transcription Factor Binding Sites in Vertebrate Genomes

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Loots, Gabriela G. [LLNL; Ovcharenko, I. [LLNL

    Evolutionary conservation of DNA sequences provides a tool for the identification of functional elements in genomes. This database of evolutionary conserved regions (ECRs) in vertebrate genomes features a database of syntenic blocks that recapitulate the evolution of rearrangements in vertebrates and a comprehensive collection of promoters in all vertebrate genomes generated using multiple sources of gene annotation. The database also contains a collection of annotated transcription factor binding sites (TFBSs) in evolutionary conserved and promoter elements. ECRbase currently includes human, rhesus macaque, dog, opossum, rat, mouse, chicken, frog, zebrafish, and fugu genomes. (taken from paper in Journal: Bioinformatics, November 7, 2006, pp. 122-124

  12. Find Standards

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    API ASCE ASHRAE ASME ASME-BPVC ASQ ASSE ASTM AWS CGA standards - contact Timothy Lopez (timlopez@lanl.gov), Ben Swartz (abswartz@lanl.gov), or Roberto Trujillo (robertot@lanl.gov) ...

  13. Crystal structure of enterococcus faecalis sly A-like transcriptional factor.

    SciTech Connect (OSTI)

    Wu, R.; Zhang, R.; Zagnitko, O.; Dementieva, I.; Maltsev, N.; Watson, J. D.; Laskowski, R.; Gornicki, P.; Joachimiak, A.; Univ. of Chicago; European Bioinformatics Inst.

    2003-05-30

    The crystal structure of a SlyA transcriptional regulator at 1.6 {angstrom} resolution is presented, and structural relationships between members of the MarR/SlyA family are discussed. The SlyA family, which includes SlyA, Rap, Hor, and RovA proteins, is widely distributed in bacterial and archaeal genomes. Current evidence suggests that SlyA-like factors act as repressors, activators, and modulators of gene transcription. These proteins have been shown to up-regulate the expression of molecular chaperones, acid-resistance proteins, and cytolysin, and down-regulate several biosynthetic enzymes. The structure of SlyA from Enterococcus faecalis, determined as a part of an ongoing structural genomics initiative (www.mcsg.anl.gov), revealed the same winged helix DNA-binding motif that was recently found in the MarR repressor from Escherichia coli and the MexR repressor from Pseudomonas aeruginosa, a sequence homologue of MarR. Phylogenetic analysis of the MarR/SlyA family suggests that Sly is placed between the SlyA and MarR subfamilies and shows significant sequence similarity to members of both subfamilies.

  14. mRNA Transcript Abundance during Plant Growth and the Influence of Li+ Exposure

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Duff, M. C.; Kuhne, W. W.; Halverson, N. V.; Chang, C. -S.; Kitamura, E.; Hawthorn, L.; Martinez, N. E.; Stafford, C.; Milliken, C. E.; Caldwell, E. F.; et al

    2014-10-23

    Lithium (Li) toxicity in plants is, at a minimum, a function of Li+ concentration, exposure time, species and growth conditions. Most plant studies with Li+ focus on short-term acute exposures. This study examines short- and long-term effects of Li+ exposure in Arabidopsis with Li+ uptake studies and measured shoot mRNA transcript abundance levels in treated and control plants. Stress, pathogen-response and arabinogalactan protein genes were typically more up-regulated in older (chronic, low level) Li+-treatment plants and in the much younger plants from acute high-level exposures. The gene regulation behavior of high-level Li+ resembled prior studies due to its influence on:more »inositol synthesis, 1-aminocyclopropane-1-carboxylate synthases and membrane ion transport. In contrast, chronically-exposed plants had gene regulation responses that were indicative of pathogen, cold, and heavy-metal stress, cell wall degradation, ethylene production, signal transduction, and calcium-release modulation. Acute Li+ exposure phenocopies magnesium-deficiency symptoms and is associated with elevated expression of stress response genes that could lead to consumption of metabolic and transcriptional energy reserves and the dedication of more resources to cell development. In contrast, chronic Li+ exposure increases expression signal transduction genes. The identification of new Li+-sensitive genes and a gene-based “response plan” for acute and chronic Li+ exposure are delineated.« less

  15. Extensive cross-regulation of post-transcriptional regulatory networks in Drosophila

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Stoiber, Marcus H.; Olson, Sara; May, Gemma E.; Duff, Michael O.; Manent, Jan; Obar, Robert; Guruharsha, K. G.; Bickel, Peter J.; Artavanis-Tsakonas, Spyros; Brown, James B.; et al

    2015-08-20

    In eukaryotic cells, RNAs exist as ribonucleoprotein particles (RNPs). Despite the importance of these complexes in many biological processes, including splicing, polyadenylation, stability, transportation, localization, and translation, their compositions are largely unknown. We affinity-purified 20 distinct RNA-binding proteins (RBPs) from cultured Drosophila melanogaster cells under native conditions and identified both the RNA and protein compositions of these RNP complexes. We identified “high occupancy target” (HOT) RNAs that interact with the majority of the RBPs we surveyed. HOT RNAs encode components of the nonsense-mediated decay and splicing machinery, as well as RNA-binding and translation initiation proteins. The RNP complexes contain proteinsmore » and mRNAs involved in RNA binding and post-transcriptional regulation. Genes with the capacity to produce hundreds of mRNA isoforms, ultracomplex genes, interact extensively with heterogeneous nuclear ribonuclear proteins (hnRNPs). Our data are consistent with a model in which subsets of RNPs include mRNA and protein products from the same gene, indicating the widespread existence of auto-regulatory RNPs. Lastly, from the simultaneous acquisition and integrative analysis of protein and RNA constituents of RNPs, we identify extensive cross-regulatory and hierarchical interactions in post-transcriptional control.« less

  16. Extensive cross-regulation of post-transcriptional regulatory networks in Drosophila

    SciTech Connect (OSTI)

    Stoiber, Marcus H.; Olson, Sara; May, Gemma E.; Duff, Michael O.; Manent, Jan; Obar, Robert; Guruharsha, K. G.; Bickel, Peter J.; Artavanis-Tsakonas, Spyros; Brown, James B.; Graveley, Brenton R.; Celniker, Susan E.

    2015-08-20

    In eukaryotic cells, RNAs exist as ribonucleoprotein particles (RNPs). Despite the importance of these complexes in many biological processes, including splicing, polyadenylation, stability, transportation, localization, and translation, their compositions are largely unknown. We affinity-purified 20 distinct RNA-binding proteins (RBPs) from cultured Drosophila melanogaster cells under native conditions and identified both the RNA and protein compositions of these RNP complexes. We identified “high occupancy target” (HOT) RNAs that interact with the majority of the RBPs we surveyed. HOT RNAs encode components of the nonsense-mediated decay and splicing machinery, as well as RNA-binding and translation initiation proteins. The RNP complexes contain proteins and mRNAs involved in RNA binding and post-transcriptional regulation. Genes with the capacity to produce hundreds of mRNA isoforms, ultracomplex genes, interact extensively with heterogeneous nuclear ribonuclear proteins (hnRNPs). Our data are consistent with a model in which subsets of RNPs include mRNA and protein products from the same gene, indicating the widespread existence of auto-regulatory RNPs. Lastly, from the simultaneous acquisition and integrative analysis of protein and RNA constituents of RNPs, we identify extensive cross-regulatory and hierarchical interactions in post-transcriptional control.

  17. Reprogramming of human fibroblasts to pluripotent stem cells using mRNA of four transcription factors

    SciTech Connect (OSTI)

    Yakubov, Eduard; Rechavi, Gidi; Rozenblatt, Shmuel; Givol, David

    2010-03-26

    Reprogramming of differentiated cells into induced pluripotent cells (iPS) was accomplished in 2006 by expressing four, or less, embryonic stem cell (ESC)-specific transcription factors. Due to the possible danger of DNA damage and the potential tumorigenicity associated with such DNA damage, attempts were made to minimize DNA integration by the vectors involved in this process without complete success. Here we present a method of using RNA transfection as a tool for reprogramming human fibroblasts to iPS. We used RNA synthesized in vitro from cDNA of the same reprogramming four transcription factors. After transfection of the RNA, we show intracellular expression and nuclear localization of the respective proteins in at least 70% of the cells. We used five consecutive transfections to support continuous protein expression resulting in the formation of iPS colonies that express alkaline phosphatase and several ESC markers and that can be expanded. This method completely avoids DNA integration and may be developed to replace the use of DNA vectors in the formation of iPS.

  18. mRNA Transcript Abundance during Plant Growth and the Influence of Li+ Exposure

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Duff, M. C.; Kuhne, W. W.; Halverson, N. V.; Chang, C. -S.; Kitamura, E.; Hawthorn, L.; Martinez, N. E.; Stafford, C.; Milliken, C. E.; Caldwell, E. F.; et al

    2014-10-23

    Lithium (Li) toxicity in plants is, at a minimum, a function of Li+ concentration, exposure time, species and growth conditions. Most plant studies with Li+ focus on short-term acute exposures. This study examines short- and long-term effects of Li+ exposure in Arabidopsis with Li+ uptake studies and measured shoot mRNA transcript abundance levels in treated and control plants. Stress, pathogen-response and arabinogalactan protein genes were typically more up-regulated in older (chronic, low level) Li+-treatment plants and in the much younger plants from acute high-level exposures. The gene regulation behavior of high-level Li+ resembled prior studies due to its influence on:more » inositol synthesis, 1-aminocyclopropane-1-carboxylate synthases and membrane ion transport. In contrast, chronically-exposed plants had gene regulation responses that were indicative of pathogen, cold, and heavy-metal stress, cell wall degradation, ethylene production, signal transduction, and calcium-release modulation. Acute Li+ exposure phenocopies magnesium-deficiency symptoms and is associated with elevated expression of stress response genes that could lead to consumption of metabolic and transcriptional energy reserves and the dedication of more resources to cell development. In contrast, chronic Li+ exposure increases expression signal transduction genes. The identification of new Li+-sensitive genes and a gene-based “response plan” for acute and chronic Li+ exposure are delineated.« less

  19. New DOE Report Finds Wind Power Can Serve as Cost-Effective Long...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finds Wind Power Can Serve as Cost-Effective Long-Term Hedge Against Natural Gas Price Increases New DOE Report Finds Wind Power Can Serve as Cost-Effective Long-Term Hedge Against ...

  20. Finding Where the Missing Proton Goes in Water | U.S. DOE Office...

    Office of Science (SC) Website

    Finding Where the Missing Proton Goes in Water Basic Energy Sciences (BES) BES Home About ... Finding Where the Missing Proton Goes in Water Researchers tackle a grand challenge by ...

  1. Report on Hydrogen Storage Panel Findings in DOE-BES Sponsored...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Hydrogen Storage Panel Findings in DOE-BES Sponsored Workshop on Basic Research for Hydrogen Production, Storage and Use Report on Hydrogen Storage Panel Findings in DOE-BES...

  2. Fact #872: May 11, 2015 Study Finds More than 60% of Millennials...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    11, 2015 Study Finds More than 60% of Millennials and Generation Xers Use the Internet to ... Fact 872: May 11, 2015 Study Finds More than 60% of Millennials and Generation Xers Use ...

  3. EA-1800: Notice of Availability of the Finding of No Significant...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Notice of Availability of the Finding of No Significant Impact Monarch Warren County Wind Turbine Project The U.S. Department of Energy (DOE) has issued a Finding of No...

  4. Alaska Oil and Gas Finding of Best Interest | Open Energy Information

    Open Energy Info (EERE)

    Oil and Gas Finding of Best Interest Jump to: navigation, search OpenEI Reference LibraryAdd to library Web Site: Alaska Oil and Gas Finding of Best Interest Author Alaska...

  5. Scientists at ALS Find New Path to More Efficient Organic Solar...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Scientists at ALS Find New Path to More Efficient Organic Solar Cells Scientists at ALS Find New Path to More Efficient Organic Solar Cells Print Monday, 07 January 2013 00:00 ...

  6. EA-1945: Finding of No Significant Impact and Mitigation Action Plan |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Finding of No Significant Impact and Mitigation Action Plan EA-1945: Finding of No Significant Impact and Mitigation Action Plan Northern Mid-Columbia Joint Project; Grant, Douglas, and Chelan Counties, Washington Bonneville Power Administration (BPA) issued a finding of no significant impact, which includes a floodplain statement of findings, for the proposed Northern Mid-Columbia Joint Project, a 230-kilvolt transmission line proposed by BPA and the Public Utility

  7. Report Summary: Hammer Testing Findings for Solid-State Lighting Luminaires

    SciTech Connect (OSTI)

    2014-01-01

    Brief summary of DOE LED Systems Reliability Consortium's Hammer Testing Findings for Solid-State Lighting Luminaires report.

  8. Summary of Findings: Peer Review of the FY2001 GPRA Assumptions

    Broader source: Energy.gov [DOE]

    Summary of Findings: Peer Review of the FY2001 GPRA Assumptions, Report to National Renewable Energy Laboratory, February 29, 2000.

  9. Summary of Findings: Peer Review of the FY2000 GPRA Assumptions

    Broader source: Energy.gov [DOE]

    Summary of Findings: Peer Review of the FY2000 GPRA Assumptions, Report to National Renewable Energy Laboratory, March 1999.

  10. Oestradiol reduces Liver Receptor Homolog-1 mRNA transcript stability in breast cancer cell lines

    SciTech Connect (OSTI)

    Lazarus, Kyren A.; Environmental and Biotechnology Centre, Swinburne University, Hawthorn, Victoria 3122 ; Zhao, Zhe; Knower, Kevin C.; To, Sarah Q.; Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3168 ; Chand, Ashwini L.; Clyne, Colin D.

    2013-08-30

    Highlights: LRH-1 is an orphan nuclear receptor that regulates tumor proliferation. In breast cancer, high mRNA expression is associated with ER+ status. In ER?ve cells, despite very low mRNA, we found abundant LRH-1 protein. Our data show distinctly different LRH-1 protein isoforms in ER? and ER+ breast cancer cells. This is due to differences in LRH-1 mRNA and protein stability rates. -- Abstract: The expression of orphan nuclear receptor Liver Receptor Homolog-1 (LRH-1) is elevated in breast cancer and promotes proliferation, migration and invasion in vitro. LRH-1 expression is regulated by oestrogen (E{sub 2}), with LRH-1 mRNA transcript levels higher in oestrogen receptor ? (ER?) positive (ER+) breast cancer cells compared to ER? cells. However, the presence of LRH-1 protein in ER? cells suggests discordance between mRNA transcript levels and protein expression. To understand this, we investigated the impact of mRNA and protein stability in determining LRH-1 protein levels in breast cancer cells. LRH-1 transcript levels were significantly higher in ER+ versus ER? breast cancer cells lines; however LRH-1 protein was expressed at similar levels. We found LRH-1 mRNA and protein was more stable in ER? compared to ER+ cell lines. The tumor-specific LRH-1 variant isoform, LRH-1v4, which is highly responsive to E{sub 2}, showed increased mRNA stability in ER? versus ER+ cells. In addition, in MCF-7 and T47-D cell lines, LRH-1 total mRNA stability was reduced with E{sub 2} treatment, this effect mediated by ER?. Our data demonstrates that in ER? cells, increased mRNA and protein stability contribute to the abundant protein expression levels. Expression and immunolocalisation of LRH-1 in ER? cells as well as ER? tumors suggests a possible role in the development of ER? tumors. The modulation of LRH-1 bioactivity may therefore be beneficial as a treatment option in both ER? and ER+ breast cancer.

  11. On-chip single-copy real-time reverse-transcription PCR in isolated picoliter droplets

    SciTech Connect (OSTI)

    Beer, N R; Wheeler, E; Lee-Houghton, L; Watkins, N; Nasarabadi, S; Hebert, N; Leung, P; Arnold, D; Bailey, C; Colston, B

    2007-12-19

    The first lab-on-chip system for picoliter droplet generation and RNA isolation, followed by reverse transcription, and PCR amplification with real-time fluorescence detection in the trapped droplets has been developed. The system utilized a shearing T-junction in a fused silica device to generate a stream of monodisperse picoliter-scale droplets that were isolated from the microfluidic channel walls and each other by the oil phase carrier. An off-chip valving system stopped the droplets on-chip, allowing thermal cycling for reverse transcription and subsequent PCR amplification without droplet motion. This combination of the established real-time reverse transcription-PCR assay with digital microfluidics is ideal for isolating single-copy RNA and virions from a complex environment, and will be useful in viral discovery and gene-profiling applications.

  12. POSTED BY JOB NAME FIELD OFFICE U.S. DEPARTMENT OF ENERGY ADP TRANSCRIPTION SHEET

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    1 1 1 1 1 1 POSTED BY JOB NAME FIELD OFFICE U.S. DEPARTMENT OF ENERGY ADP TRANSCRIPTION SHEET INTERNAL PROJECT TRANSFERS (See instructions for provisions regarding confidentiality.) ACT. CODE PROC. CODE 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 1 SHIPPER RIS TRAN. CODE TO PROJECT NUMBER RECEIVER RIS INTERNAL

  13. POSTED BY JOB NAME FIELD OFFICE U.S. DEPARTMENT OF ENERGY ADP TRANSCRIPTION SHEET

    National Nuclear Security Administration (NNSA)

    1 1 1 1 1 1 1 POSTED BY JOB NAME FIELD OFFICE U.S. DEPARTMENT OF ENERGY ADP TRANSCRIPTION SHEET INTERNAL PROJECT TRANSFERS (See instructions for provisions regarding confidentiality.) ACT. CODE PROC. CODE 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 1 SHIPPER RIS TRAN. CODE TO PROJECT NUMBER RECEIVER RIS INTERNAL

  14. Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus

    DOE Patents [OSTI]

    Dai, Ziyu; Lasure, Linda L; Magnuson, Jon K

    2014-05-27

    The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus.

  15. Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus

    DOE Patents [OSTI]

    Dai, Ziyu; Lasure, Linda L.; Magnuson, Jon K.

    2008-11-11

    The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus.

  16. Isolated Fungal Promoters and Gene Transcription Terminators and Methods of Protein and Chemical Production in a Fungus

    DOE Patents [OSTI]

    Dai, Ziyu; Lasure, Linda L.; Magnuson, Jon K.

    2008-11-11

    The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus.

  17. A novel transcriptional regulator of L-arabinose utilization in human gut bacteria

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Chang, Changsoo; Tesar, Christine; Li, Xiaoqing; Kim, Youngchang; Rodionov, Dmitry A.; Joachimiak, Andrzej

    2015-10-04

    We report that carbohydrate metabolism plays a crucial role in the ecophysiology of human gut microbiota. Mechanisms of transcriptional regulation of sugar catabolism in commensal and prevalent human gut bacteria such as Bacteroides thetaiotaomicron remain mostly unknown. By a combination of bioinformatics and experimental approaches, we have identified an NrtR family transcription factor (BT0354 in B. thetaiotaomicron, BtAraR) as a novel regulator controlling the arabinose utilization genes. L-arabinose was confirmed to be a negative effector of BtAraR. We have solved the crystal structures of the apo and L-arabinose-bound BtAraR proteins, as well as the complex of apo-protein with a specificmore » DNA operator. BtAraR forms a homodimer with each subunit comprised of the ligand-binding Nudix hydrolase-like domain and the DNA-binding winged-helix-turn-helix (wHTH) domain. We have identified the residues involved in binding of L-arabinose and recognition of DNA. The majority of these residues are well conserved in the AraR orthologs in Bacteroidetes. In the structure of the BtAraR–DNA complex, we found the unique interaction of arginine intercalating its guanidinum moiety into the base pair stacking of B-DNA. L-arabinose binding induces movement of wHTH domains, resulting in a conformation unsuitable for DNA binding. Furthermore, our analysis facilitates reconstruction of the metabolic and regulatory networks involved in carbohydrate utilization in human gut Bacteroides.« less

  18. A novel transcriptional regulator of L-arabinose utilization in human gut bacteria

    SciTech Connect (OSTI)

    Chang, Changsoo; Tesar, Christine; Li, Xiaoqing; Kim, Youngchang; Rodionov, Dmitry A.; Joachimiak, Andrzej

    2015-10-04

    We report that carbohydrate metabolism plays a crucial role in the ecophysiology of human gut microbiota. Mechanisms of transcriptional regulation of sugar catabolism in commensal and prevalent human gut bacteria such as Bacteroides thetaiotaomicron remain mostly unknown. By a combination of bioinformatics and experimental approaches, we have identified an NrtR family transcription factor (BT0354 in B. thetaiotaomicron, BtAraR) as a novel regulator controlling the arabinose utilization genes. L-arabinose was confirmed to be a negative effector of BtAraR. We have solved the crystal structures of the apo and L-arabinose-bound BtAraR proteins, as well as the complex of apo-protein with a specific DNA operator. BtAraR forms a homodimer with each subunit comprised of the ligand-binding Nudix hydrolase-like domain and the DNA-binding winged-helix-turn-helix (wHTH) domain. We have identified the residues involved in binding of L-arabinose and recognition of DNA. The majority of these residues are well conserved in the AraR orthologs in Bacteroidetes. In the structure of the BtAraR–DNA complex, we found the unique interaction of arginine intercalating its guanidinum moiety into the base pair stacking of B-DNA. L-arabinose binding induces movement of wHTH domains, resulting in a conformation unsuitable for DNA binding. Furthermore, our analysis facilitates reconstruction of the metabolic and regulatory networks involved in carbohydrate utilization in human gut Bacteroides.

  19. Ligand-specific transcriptional mechanisms underlie aryl hydrocarbon receptor-mediated developmental toxicity of oxygenated PAHs

    SciTech Connect (OSTI)

    Goodale, B. C.; La Du, J.; Tilton, S. C.; Sullivan, C. M.; Bisson, W. H.; Waters, K. M.; Tanguay, R. L.

    2015-07-03

    Polycyclic aromatic hydrocarbons (PAHs) are priority environmental contaminants that exhibit mutagenic, carcinogenic, proinflammatory, and teratogenic properties. Oxygen-substituted PAHs (OPAHs) are formed during combustion processes and via phototoxidation and biological degradation of parent (unsubstituted) PAHs. Despite their prevalence both in contaminated industrial sites and in urban air, OPAH mechanisms of action in biological systems are relatively understudied. Like parent PAHs, OPAHs exert structure-dependent mutagenic activities and activation of the aryl hydrocarbon receptor (AHR) and cytochrome p450 metabolic pathway. Four-ring OPAHs 1,9-benz-10-anthrone (BEZO) and benz(a)anthracene-7,12-dione (7,12-B[a]AQ) cause morphological aberrations and induce markers of oxidative stress in developing zebrafish with similar potency, but only 7,12-B[a]AQ induces robust Cyp1a protein expression. We investigated the role of the AHR in mediating the toxicity of BEZO and 7,12-B[a]AQ, and found that knockdown of AHR2 rescued developmental effects caused by both compounds. Using RNA-seq and molecular docking, we identified transcriptional responses that precede developmental toxicity induced via differential interaction with AHR2. Redox-homeostasis genes were affected similarly by these OPAHs, while 7,12-B[a]AQ preferentially activated phase 1 metabolism and BEZO uniquely decreased visual system genes. Analysis of biological functions and upstream regulators suggests that BEZO is a weak AHR agonist, but interacts with other transcriptional regulators to cause developmental toxicity in an AHR-dependent manner. Furthermore, identifying ligand-dependent AHR interactions and signaling pathways is essential for understanding toxicity of this class of environmentally relevant compounds.

  20. An integrated approach to reconstructing genome-scale transcriptional regulatory networks

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Imam, Saheed; Noguera, Daniel R.; Donohue, Timothy J.; Leslie, Christina

    2015-02-27

    Transcriptional regulatory networks (TRNs) program cells to dynamically alter their gene expression in response to changing internal or environmental conditions. In this study, we develop a novel workflow for generating large-scale TRN models that integrates comparative genomics data, global gene expression analyses, and intrinsic properties of transcription factors (TFs). An assessment of this workflow using benchmark datasets for the well-studied γ-proteobacterium Escherichia coli showed that it outperforms expression-based inference approaches, having a significantly larger area under the precision-recall curve. Further analysis indicated that this integrated workflow captures different aspects of the E. coli TRN than expression-based approaches, potentially making themmore » highly complementary. We leveraged this new workflow and observations to build a large-scale TRN model for the α-Proteobacterium Rhodobacter sphaeroides that comprises 120 gene clusters, 1211 genes (including 93 TFs), 1858 predicted protein-DNA interactions and 76 DNA binding motifs. We found that ~67% of the predicted gene clusters in this TRN are enriched for functions ranging from photosynthesis or central carbon metabolism to environmental stress responses. We also found that members of many of the predicted gene clusters were consistent with prior knowledge in R. sphaeroides and/or other bacteria. Experimental validation of predictions from this R. sphaeroides TRN model showed that high precision and recall was also obtained for TFs involved in photosynthesis (PpsR), carbon metabolism (RSP_0489) and iron homeostasis (RSP_3341). In addition, this integrative approach enabled generation of TRNs with increased information content relative to R. sphaeroides TRN models built via other approaches. We also show how this approach can be used to simultaneously produce TRN models for each related organism used in the comparative genomics analysis. Our results highlight the advantages of

  1. Ligand-specific transcriptional mechanisms underlie aryl hydrocarbon receptor-mediated developmental toxicity of oxygenated PAHs

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Goodale, B. C.; Geisel School of Medicine at Dartmouth, Hanover, NH; La Du, J.; Tilton, S. C.; Pacific Northwest National Lab.; Sullivan, C. M.; Bisson, W. H.; Waters, K. M.; Tanguay, R. L.

    2015-07-03

    Polycyclic aromatic hydrocarbons (PAHs) are priority environmental contaminants that exhibit mutagenic, carcinogenic, proinflammatory, and teratogenic properties. Oxygen-substituted PAHs (OPAHs) are formed during combustion processes and via phototoxidation and biological degradation of parent (unsubstituted) PAHs. Despite their prevalence both in contaminated industrial sites and in urban air, OPAH mechanisms of action in biological systems are relatively understudied. Like parent PAHs, OPAHs exert structure-dependent mutagenic activities and activation of the aryl hydrocarbon receptor (AHR) and cytochrome p450 metabolic pathway. Four-ring OPAHs 1,9-benz-10-anthrone (BEZO) and benz(a)anthracene-7,12-dione (7,12-B[a]AQ) cause morphological aberrations and induce markers of oxidative stress in developing zebrafish with similar potency, butmore » only 7,12-B[a]AQ induces robust Cyp1a protein expression. We investigated the role of the AHR in mediating the toxicity of BEZO and 7,12-B[a]AQ, and found that knockdown of AHR2 rescued developmental effects caused by both compounds. Using RNA-seq and molecular docking, we identified transcriptional responses that precede developmental toxicity induced via differential interaction with AHR2. Redox-homeostasis genes were affected similarly by these OPAHs, while 7,12-B[a]AQ preferentially activated phase 1 metabolism and BEZO uniquely decreased visual system genes. Analysis of biological functions and upstream regulators suggests that BEZO is a weak AHR agonist, but interacts with other transcriptional regulators to cause developmental toxicity in an AHR-dependent manner. Furthermore, identifying ligand-dependent AHR interactions and signaling pathways is essential for understanding toxicity of this class of environmentally relevant compounds.« less

  2. An integrated approach to reconstructing genome-scale transcriptional regulatory networks

    SciTech Connect (OSTI)

    Imam, Saheed; Noguera, Daniel R.; Donohue, Timothy J.; Leslie, Christina

    2015-02-27

    Transcriptional regulatory networks (TRNs) program cells to dynamically alter their gene expression in response to changing internal or environmental conditions. In this study, we develop a novel workflow for generating large-scale TRN models that integrates comparative genomics data, global gene expression analyses, and intrinsic properties of transcription factors (TFs). An assessment of this workflow using benchmark datasets for the well-studied γ-proteobacterium Escherichia coli showed that it outperforms expression-based inference approaches, having a significantly larger area under the precision-recall curve. Further analysis indicated that this integrated workflow captures different aspects of the E. coli TRN than expression-based approaches, potentially making them highly complementary. We leveraged this new workflow and observations to build a large-scale TRN model for the α-Proteobacterium Rhodobacter sphaeroides that comprises 120 gene clusters, 1211 genes (including 93 TFs), 1858 predicted protein-DNA interactions and 76 DNA binding motifs. We found that ~67% of the predicted gene clusters in this TRN are enriched for functions ranging from photosynthesis or central carbon metabolism to environmental stress responses. We also found that members of many of the predicted gene clusters were consistent with prior knowledge in R. sphaeroides and/or other bacteria. Experimental validation of predictions from this R. sphaeroides TRN model showed that high precision and recall was also obtained for TFs involved in photosynthesis (PpsR), carbon metabolism (RSP_0489) and iron homeostasis (RSP_3341). In addition, this integrative approach enabled generation of TRNs with increased information content relative to R. sphaeroides TRN models built via other approaches. We also show how this approach can be used to simultaneously produce TRN models for each related organism used in the comparative genomics analysis. Our results highlight the advantages of integrating

  3. Tour of Zero: Helping Customers Find Better Homes at Lower Costs Webinar

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    (Text Version) | Department of Energy Tour of Zero: Helping Customers Find Better Homes at Lower Costs Webinar (Text Version) Tour of Zero: Helping Customers Find Better Homes at Lower Costs Webinar (Text Version) Below is the text version of the webinar Tour of Zero: Helping Customers Find Better Homes at Lower Costs, presented in October 2015. Watch the presentation. Lindsay Parker: Hi, everyone. Welcome to the Department of Energy Zero Energy Ready Home technical training webinar series.

  4. EA-1950: Finding of No Significant Impact and Mitigation Action Plan |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy 0: Finding of No Significant Impact and Mitigation Action Plan EA-1950: Finding of No Significant Impact and Mitigation Action Plan Grand Coulee-Creston Transmission Line Rebuild; Grant and Lincoln Counties, Washington Bonneville Power Administration issued a finding of no significant impact and a mitigation action plan for rebuilding approximately 28 miles of the Grand Coulee-Creston No. 1 115-kilovolt (kV) transmission line between Coulee Dam in Grant County and

  5. EA-2006: Finding of No Significant Impact and Mitigation Action Plan |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Finding of No Significant Impact and Mitigation Action Plan EA-2006: Finding of No Significant Impact and Mitigation Action Plan Columbia Estuary Ecosystem Restoration Program Bonneville Power Administration issued a FONSI and a floodplain and wetland statement of findings for a proposed program of actions to help restore ecological structure, function, and biodiversity within the Columbia River estuary. The mitigation measures listed in the Mitigation Action Plan and

  6. Focus Series: Program Finds Community "Readiness" Is the Key to More

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Retrofits | Department of Energy Program Finds Community "Readiness" Is the Key to More Retrofits Focus Series: Program Finds Community "Readiness" Is the Key to More Retrofits Focus Series: Program Finds Community "Readiness" Is the Key to More Retrofits, a document posted by the U.S. Department of Energy's (DOE'S) Better Buildings Neighborhood Program. Focus Series: Michigan (284.83 KB) More Documents & Publications Better Buildings Network View | June

  7. Using regulations.gov to find dockets and documents | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Using regulations.gov to find dockets and documents Using regulations.gov to find dockets and documents A document from the U.S. Department of Energy's Office of Energy Efficiency and Renewable Energy, Building Technologies Office, explaining how to use Regulations.gov to find dockets and documents. as_accessing_documents.pdf (700.83 KB) More Documents & Publications Regulations.gov - Proposed Rule Document ISSUANCE 2015-08-21: Energy Conservation Program for Consumer Products: Energy

  8. Business Case for Small Modular Reactors Report on Findings | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Business Case for Small Modular Reactors Report on Findings Business Case for Small Modular Reactors Report on Findings This study assesses the market for SMRs and develops a business case to identify incentives, policies, and programs that can be effectively implemented and have significant impact on the commercialization of SMRs. Business Case for Small Modular Reactors Report on Findings to the U.S. Department of Energy (666.81 KB) More Documents & Publications A Strategic

  9. Idaho IC 61-1701, Legislative Purposes and Findings for the Siting...

    Open Energy Info (EERE)

    701, Legislative Purposes and Findings for the Siting of Certain Electrical Transmission Facilities Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal...

  10. Clark Fork River Delta Restoration Project 1 Finding of No Significant...

    Broader source: Energy.gov (indexed) [DOE]

    findings for its proposal to provide partial funding to the Idaho Department of Fish and Game's (IDFG) Clark Fork River Delta Restoration Project. The project would involve...

  11. How to find fees, taxes, and other charges built into rate in...

    Open Energy Info (EERE)

    and other charges built into rate in different locations Home > Groups > Utility Rate Hi, I am looking to find the rate charges beyond demand, generation, and transmission that...

  12. Finding Hidden Oil and Gas Reserves | U.S. DOE Office of Science...

    Office of Science (SC) Website

    Finding Hidden Oil and Gas Reserves Basic Energy Sciences (BES) BES Home About Research ... Michael Commer LBNL MCommer@lbl.gov Funding Basic Research: DOE Office of Science ...

  13. OSTIblog Articles in the r&d findings Topic | OSTI, US Dept of...

    Office of Scientific and Technical Information (OSTI)

    findings Topic OSTI's Contributions Highlighted in DOE Open Government Plan by Dr. Walt Warnick 11 May, 2010 in Science Communications By Walt Warnick and Peter Lincoln The ...

  14. NREL Finds a New Cellulose Digestion Mechanism by a Fast-eating...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    almost twice as fast as the current leading component cellulase enzyme on the market. ... A paper reporting this finding, "Revealing Nature's Cellulase Diversity: The Digestion ...

  15. Six Places to Find Help with Your Energy Costs | Department of...

    Energy Savers [EERE]

    and Efficiency (DSIRE). Here you'll find a comprehensive listing of available rebates, loans, grants, and tax incentives for renewable energy technologies and energy...

  16. Focus Series: Program Finds Community ReadinessŽ Is the Key...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Learn more at betterbuildings.energy.govneighborhoods BETTERBUILDINGS FOR MICHIGAN Program Finds Community ... neighborhood "sweeps" in 58 communities across the state. ...

  17. Summary of Findings: Peer Review of the FY2003 GPRA Assumptions

    Broader source: Energy.gov [DOE]

    Summary of Findings: Peer Review of the FY ‘03 GPRA Assumptions, Report to National Renewable Energy Laboratory, Washington, D.C., April 3, 2002.

  18. EA-1611-S1: Finding of No Significant Impact | Department of...

    Broader source: Energy.gov (indexed) [DOE]

    Project, Logan County, Colorado DOE's Western Area Power Administration issued a Finding of No Significant Impact for the proposed expansion of Colorado Highlands Wind Project,...

  19. EA-1815: DOE Notice of Availability of the Finding of No Significant...

    Broader source: Energy.gov (indexed) [DOE]

    environmental impacts associated with the: Cuyahoga County Agricultural Society Wind Energy Project; Berea, Cuyahoga County, Ohio. Notice of Availability of a Finding of No...

  20. EA-1440-S1: Finding of No Significant Impact | Department of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    EA-1440-S1: Finding of No Significant Impact National Renewable Energy Laboratory's South Table Mountain Complex (Golden, Colorado) (see also DOEEA-1440-S1 DOEEA-1609; DOE...

  1. Creating a Better Way to Find Out "When" | U.S. DOE Office of...

    Office of Science (SC) Website

    Basic Energy Sciences (BES) BES Home About Research Facilities Science Highlights Benefits ... Creating a Better Way to Find Out "When" Computer algorithm recovers histories and ...

  2. AS-2, a novel inhibitor of p53-dependent apoptosis, prevents apoptotic mitochondrial dysfunction in a transcription-independent manner and protects mice from a lethal dose of ionizing radiation

    SciTech Connect (OSTI)

    Morita, Akinori; Ariyasu, Shinya; Wang, Bing; Asanuma, Tetsuo; Onoda, Takayoshi; Sawa, Akiko; Tanaka, Kaoru; Takahashi, Ippei; Togami, Shotaro; Nenoi, Mitsuru; Inaba, Toshiya; Aoki, Shin

    2014-08-08

    Highlights: A bidentate HQ derivative, AS-2, suppresses p53-dependent apoptosis by DNA damage. AS-2 does not significantly affect nuclear p53 response. UV-excited blue emission of AS-2 clearly showed its extranuclear localization. AS-2 prevents mitochondrial dysfunction despite the increase of mitochondrial p53. AS-2 protects mice from a radiation dose that causes lethal hematopoietic syndrome. - Abstract: In a previous study, we reported that some tetradentate zinc(II) chelators inhibit p53 through the denaturation of its zinc-requiring structure but a chelator, Bispicen, a potent inhibitor of in vitro apoptosis, failed to show any efficient radioprotective effect against irradiated mice because the toxicity of the chelator to mice. The unsuitability of using tetradentate chelators as radioprotectors prompted us to undertake a more extensive search for p53-inhibiting agents that are weaker zinc(II) chelators and therefore less toxic. Here, we show that an 8-hydroxyquinoline (8HQ) derivative, AS-2, suppresses p53-dependent apoptosis through a transcription-independent mechanism. A mechanistic study using cells with different p53 characteristics revealed that the suppressive effect of AS-2 on apoptosis is specifically mediated through p53. In addition, AS-2 was less effective in preventing p53-mediated transcription-dependent events than pifithrin-? (PFT?), an inhibitor of transcription-independent apoptosis by p53. Fluorescence visualization of the extranuclear distribution of AS-2 also supports that it is ineffective on the transcription-dependent pathway. Further investigations revealed that AS-2 suppressed mitochondrial apoptotic events, such as the mitochondrial release of intermembrane proteins and the loss of mitochondrial membrane potential, although AS-2 resulted in an increase in the mitochondrial translocation of p53 as opposed to the decrease of cytosolic p53, and did not affect the apoptotic interaction of p53 with Bcl-2. AS-2 also protected

  3. Energetic Consequences of nitrite stress in Desulfovibrio vulgarisHildenborough, inferred from global transcriptional analysis

    SciTech Connect (OSTI)

    He, Qiang; Huang, Katherine H.; He, Zhili; Alm, Eric J.; Fields,Matthew W.; Hazen, Terry C.; Arkin, Adam P.; Wall, Judy D.; Zhou, Jizhong

    2005-11-03

    Many of the proteins that are candidates for bioenergetic pathways involved with sulfate respiration in Desulfovibrio spp. have been studied, but complete pathways and overall cell physiology remain to be resolved for many environmentally relevant conditions. In order to understand the metabolism of these microorganisms under adverse environmental conditions for improved bioremediation efforts, Desulfovibrio vulgaris Hildenborough was used as a model organism to study stress response to nitrite, an important intermediate in the nitrogen cycle. Previous physiological studies demonstrated that growth was inhibited by nitrite and that nitrite reduction was observed to be the primary mechanism of detoxification. Global transcriptional profiling with whole-genome microarrays revealed coordinated cascades of responses to nitrite in pathways of energy metabolism, nitrogen metabolism, oxidative stress response, and iron homeostasis. In agreement with previous observations, nitrite-stressed cells showed a decrease in the expression of genes encoding sulfate reduction functions in addition to respiratory oxidative phosphorylation and ATP synthase activity. Consequently, the stressed cells had decreased expression of the genes encoding ATP-dependent amino acid transporters and proteins involved in translation. Other genes up-regulated in response to nitrite include the genes in the Fur regulon, which is suggested to be involved in iron homeostasis, and genes in the Per regulon, which is predicted to be responsible for oxidative stress response.

  4. Automated genomic context analysis and experimental validation platform for discovery of prokaryote transcriptional regulator functions

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Martí-Arbona, Ricardo; Mu, Fangping; Nowak-Lovato, Kristy L.; Wren, Melinda S.; Unkefer, Clifford J.; Unkefer, Pat J.

    2014-12-18

    The clustering of genes in a pathway and the co-location of functionally related genes is widely recognized in prokaryotes. We used these characteristics to predict the metabolic involvement for a Transcriptional Regulator (TR) of unknown function, identified and confirmed its biological activity. A software tool that identifies the genes encoded within a defined genomic neighborhood for the subject TR and its homologs was developed. The output lists of genes in the genetic neighborhoods, their annotated functions, the reactants/products, and identifies the metabolic pathway in which the encoded-proteins function. When a set of TRs of known function was analyzed, we observedmore » that their homologs frequently had conserved genomic neighborhoods that co-located the metabolically related genes regulated by the subject TR. We postulate that TR effectors are metabolites in the identified pathways; indeed the known effectors were present. We analyzed Bxe_B3018 from Burkholderia xenovorans, a TR of unknown function and predicted that this TR was related to the glycine, threonine and serine degradation. We tested the binding of metabolites in these pathways and for those that bound, their ability to modulate TR binding to its specific DNA operator sequence. Using rtPCR, we confirmed that methylglyoxal was an effector of Bxe_3018.« less

  5. Automated genomic context analysis and experimental validation platform for discovery of prokaryote transcriptional regulator functions

    SciTech Connect (OSTI)

    Mart-Arbona, Ricardo; Mu, Fangping; Nowak-Lovato, Kristy L.; Wren, Melinda S.; Unkefer, Clifford J.; Unkefer, Pat J.

    2014-12-18

    The clustering of genes in a pathway and the co-location of functionally related genes is widely recognized in prokaryotes. We used these characteristics to predict the metabolic involvement for a Transcriptional Regulator (TR) of unknown function, identified and confirmed its biological activity. A software tool that identifies the genes encoded within a defined genomic neighborhood for the subject TR and its homologs was developed. The output lists of genes in the genetic neighborhoods, their annotated functions, the reactants/products, and identifies the metabolic pathway in which the encoded-proteins function. When a set of TRs of known function was analyzed, we observed that their homologs frequently had conserved genomic neighborhoods that co-located the metabolically related genes regulated by the subject TR. We postulate that TR effectors are metabolites in the identified pathways; indeed the known effectors were present. We analyzed Bxe_B3018 from Burkholderia xenovorans, a TR of unknown function and predicted that this TR was related to the glycine, threonine and serine degradation. We tested the binding of metabolites in these pathways and for those that bound, their ability to modulate TR binding to its specific DNA operator sequence. Using rtPCR, we confirmed that methylglyoxal was an effector of Bxe_3018.

  6. Global transcriptional, physiological and metabolite analyses of Desulfovibrio vulgaris Hildenborough responses to salt adaptation

    SciTech Connect (OSTI)

    He, Z.; Zhou, A.; Baidoo, E.; He, Q.; Joachimiak, M. P.; Benke, P.; Phan, R.; Mukhopadhyay, A.; Hemme, C.L.; Huang, K.; Alm, E.J.; Fields, M.W.; Wall, J.; Stahl, D.; Hazen, T.C.; Keasling, J.D.; Arkin, A.P.; Zhou, J.

    2009-12-01

    The response of Desulfovibrio vulgaris Hildenborough to salt adaptation (long-term NaCl exposure) was examined by physiological, global transcriptional, and metabolite analyses. The growth of D. vulgaris was inhibited by high levels of NaCl, and the growth inhibition could be relieved by the addition of exogenous amino acids (e.g., glutamate, alanine, tryptophan) or yeast extract. Salt adaptation induced the expression of genes involved in amino acid biosynthesis and transport, electron transfer, hydrogen oxidation, and general stress responses (e.g., heat shock proteins, phage shock proteins, and oxidative stress response proteins). Genes involved in carbon metabolism, cell motility, and phage structures were repressed. Comparison of transcriptomic profiles of D. vulgaris responses to salt adaptation with those of salt shock (short-term NaCl exposure) showed some similarity as well as a significant difference. Metabolite assays showed that glutamate and alanine were accumulated under salt adaptation, suggesting that they may be used as osmoprotectants in D. vulgaris. A conceptual model is proposed to link the observed results to currently available knowledge for further understanding the mechanisms of D. vulgaris adaptation to elevated NaCl.

  7. V-057: eXtplorer "ext_find_user()" Authentication Bypass Vulnerability |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy 57: eXtplorer "ext_find_user()" Authentication Bypass Vulnerability V-057: eXtplorer "ext_find_user()" Authentication Bypass Vulnerability December 28, 2012 - 6:00am Addthis December 28 2012 - 6:00am PROBLEM: eXtplorer "ext_find_user()" Authentication Bypass Vulnerability PLATFORM: eXtplorer 2.x ABSTRACT: A vulnerability has been reported in eXtplorer, which can be exploited by malicious people to bypass certain security restrictions.

  8. Webinar: Wind-to-Hydrogen Cost Modeling and Project Findings | Department

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of Energy Wind-to-Hydrogen Cost Modeling and Project Findings Webinar: Wind-to-Hydrogen Cost Modeling and Project Findings Below is the text version of the webinar titled "Wind-to-Hydrogen Cost Modeling and Project Findings," originally presented on January 17, 2013. In addition to this text version of the audio, you can access the presentation slides. Moderator: Welcome to today's second attempt at the webinar given by NREL today. So we appreciate you guys that were patient with

  9. Sign up to find out what's next for SunShot | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Sign up to find out what's next for SunShot Sign up to find out what's next for SunShot The SunShot Initiative is a national effort to drive the costs down for solar. As we celebrate a major milestone in the industry-one million solar systems installed across America-we are also looking forward to the next several million installations. Sign up to find out how the SunShot Initiative is helping prepare the country for a solar-powered future. Enter your email address here: Subscribe After

  10. EVMS Training Snippet: 1.8 DOE Common EVMS Findings | Department of Energy

    Office of Environmental Management (EM)

    8 DOE Common EVMS Findings EVMS Training Snippet: 1.8 DOE Common EVMS Findings This EVMS Training Snippet, sponsored by the Office of Project Management (PM) discusses the common surveillance findings identified during DOE EVMS Reviews. Link to Video Presentation (13:38) | Prior Snippet (1.7) | Next Snippet (2.1) | Return to Index Slides Only (150.79 KB) Slides with Notes (1.38 MB) Key Resources PMCDP EVMS PARS IIe FPD Resource Center PM Newsletter Forms and Templates More Documents &

  11. DOE ZERH Webinar: Tour of Zero, Helping Customers Find Better Homes at

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Lower Costs | Department of Energy Tour of Zero, Helping Customers Find Better Homes at Lower Costs DOE ZERH Webinar: Tour of Zero, Helping Customers Find Better Homes at Lower Costs Are you interested in helping homebuyers find a better home at a lower cost? The DOE Tour of Zero provides the opportunity for visitors to take a virtual tour of high-performance Zero Energy Ready Homes in every major U.S. climate zone and browse extensive photographs, homeowner testimonials, lists of

  12. V-057: eXtplorer "ext_find_user()" Authentication Bypass Vulnerability |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy 7: eXtplorer "ext_find_user()" Authentication Bypass Vulnerability V-057: eXtplorer "ext_find_user()" Authentication Bypass Vulnerability December 28, 2012 - 6:00am Addthis PROBLEM: eXtplorer "ext_find_user()" Authentication Bypass Vulnerability PLATFORM: eXtplorer 2.x ABSTRACT: A vulnerability has been reported in eXtplorer, which can be exploited by malicious people to bypass certain security restrictions. REFERENCE LINKS: Secunia Advisory

  13. Leptin upregulates telomerase activity and transcription of human telomerase reverse transcriptase in MCF-7 breast cancer cells

    SciTech Connect (OSTI)

    Ren, He; Zhao, Tiansuo; Wang, Xiuchao; Gao, Chuntao; Wang, Jian; Yu, Ming; Hao, Jihui

    2010-03-26

    The aim was to analyze the mechanism of leptin-induced activity of telomerase in MCF-7 breast cancer cells. We found that leptin activated telomerase in a dose-dependent manner; leptin upregulated the expression of Human Telomerase Reverse Transcriptase (hTERT) at mRNA and protein levels; blockade of signal transducer and activator of transcription 3 (STAT3) phosphorylation significantly counteracted leptin-induced hTERT transcription and protein expression; chromatin immunoprecipitation analysis showed that leptin enhanced the binding of STAT3 to the hTERT promoter. This study uncovers a new mechanism of the proliferative effect of leptin on breast cancer cells and provides a new explanation of obesity-related breast cancer.

  14. Sertad1 encodes a novel transcriptional co-activator of SMAD1 in mouse embryonic hearts

    SciTech Connect (OSTI)

    Peng, Yin; Zhao, Shaomin; Song, Langying; Wang, Manyuan; Jiao, Kai

    2013-11-29

    Highlights: •SERTAD1 interacts with SMAD1. •Sertad1 is expressed in mouse embryonic hearts. •SERTAD1 is localized in both cytoplasm and nucleus of cardiomyocytes. •SERTAD1 enhances expression of BMP target cardiogenic genes as a SMAD1 co-activator. -- Abstract: Despite considerable advances in surgical repairing procedures, congenital heart diseases (CHDs) remain the leading noninfectious cause of infant morbidity and mortality. Understanding the molecular/genetic mechanisms underlying normal cardiogenesis will provide essential information for the development of novel diagnostic and therapeutic strategies against CHDs. BMP signaling plays complex roles in multiple cardiogenic processes in mammals. SMAD1 is a canonical nuclear mediator of BMP signaling, the activity of which is critically regulated through its interaction partners. We screened a mouse embryonic heart yeast two-hybrid library using Smad1 as bait and identified SERTAD1 as a novel interaction partner of SMAD1. SERTAD1 contains multiple potential functional domains, including two partially overlapping transactivation domains at the C terminus. The SERTAD1-SMAD1 interaction in vitro and in mammalian cells was further confirmed through biochemical assays. The expression of Sertad1 in developing hearts was demonstrated using RT-PCR, western blotting and in situ hybridization analyses. We also showed that SERTAD1 was localized in both the cytoplasm and nucleus of immortalized cardiomyocytes and primary embryonic cardiomyocyte cultures. The overexpression of SERTAD1 in cardiomyocytes not only enhanced the activity of two BMP reporters in a dose-dependent manner but also increased the expression of several known BMP/SMAD regulatory targets. Therefore, these data suggest that SERTAD1 acts as a SMAD1 transcriptional co-activator to promote the expression of BMP target genes during mouse cardiogenesis.

  15. Comparative phosphoproteomics reveals components of host cell invasion and post-transcriptional regulation during Francisella infection

    SciTech Connect (OSTI)

    Nakayasu, Ernesto S.; Tempel, Rebecca; Cambronne, Xiaolu A.; Petyuk, Vladislav A.; Jones, Marcus B.; Gritsenko, Marina A.; Monroe, Matthew E.; Yang, Feng; Smith, Richard D.; Adkins, Joshua N.; Heffron, Fred

    2013-09-22

    Francisella tularensis is a facultative intracellular bacterium that causes the deadly disease tularemia. Most evidence suggests that Francisella is not well recognized by the innate immune system that normally leads to cytokine expression and cell death. In previous work, we identified new bacterial factors that were hyper-cytotoxic to macrophages. Four of the identified hyper-cytotoxic strains (lpcC, manB, manC and kdtA) had an impaired lipopolysaccharide (LPS) synthesis and produced an exposed lipid A lacking the O-antigen. These mutants were not only hyper-cytotoxic but also were phagocytosed at much higher rates compared to the wild type parent strain. To elucidate the cellular signaling underlying this enhanced phagocytosis and cell death, we performed a large-scale comparative phosphoproteomic analysis of cells infected with wild-type and delta-lpcC F. novicida. Our data suggest that not only actin but also intermediate filaments and microtubules are important for F. novicida entry into the host cells. In addition, we observed differential phosphorylation of tristetraprolin (TTP), a key component of the mRNA-degrading machinery that controls the expression of a variety of genes including many cytokines. Infection with the delta-lpcC mutant induced the hyper-phosphorylation and inhibition of TTP, leading to the production of cytokines such as IL-1beta and TNF-alpha which may kill the host cells by triggering apoptosis. Together, our data provide new insights for Francisella invasion and a post-transcriptional mechanism that prevents the expression of host immune response factors that controls infection by this pathogen.

  16. Expression of barley SUSIBA2 transcription factor yields high-starch low-methane rice

    SciTech Connect (OSTI)

    Su, J.; Hu, C.; Yan, X.; Jin, Y.; Chen, Z.; Guan, Q.; Wang, Y.; Zhong, D.; Jansson, Georg C.; Wang, F.; Schnrer, Anna; Sun, Chuanxin

    2015-07-22

    Atmospheric methane is the second most important greenhouse gas after carbon dioxide, and is responsible for about 20% of the global warming effect since pre-industrial times. Rice paddies are the largest anthropogenic methane source and produce 7–17% of atmospheric methane. Warm waterlogged soil and exuded nutrients from rice roots provide ideal conditions for methanogenesis in paddies with annual methane emissions of 25–100-million tonnes. This scenario will be exacerbated by an expansion in rice cultivation needed to meet the escalating demand for food in the coming decades4. There is an urgent need to establish sustainable technologies for increasing rice production while reducing methane fluxes from rice paddies. However, ongoing efforts for methane mitigation in rice paddies are mainly based on farming practices and measures that are difficult to implement5. Despite proposed strategies to increase rice productivity and reduce methane emissions4,6, no high-starch low-methane-emission rice has been developed. Here we show that the addition of a single transcription factor gene, barley SUSIBA2, conferred a shift of carbon flux to SUSIBA2 rice, favouring the allocation of photosynthates to aboveground biomass over allocation to roots. The altered allocation resulted in an increased biomass and starch content in the seeds and stems, and suppressed methanogenesis, possibly through a reduction in root exudates. Three-year field trials in China demonstrated that the cultivation of SUSIBA2 rice was associated with a significant reduction in methane emissions and a decrease in rhizospheric methanogen levels. SUSIBA2 rice offers a sustainable means of providing increased starch content for food production while reducing greenhouse gas emissions from rice cultivation. Approaches to increase rice productivity and reduce methane emissions as seen in SUSIBA2 rice may be particularly beneficial in a future climate with rising temperatures resulting in increased methane

  17. Experimental Definition and Validation of Protein Coding Transcripts in Chlamydomonas reinhardtii

    SciTech Connect (OSTI)

    Kourosh Salehi-Ashtiani; Jason A. Papin

    2012-01-13

    Algal fuel sources promise unsurpassed yields in a carbon neutral manner that minimizes resource competition between agriculture and fuel crops. Many challenges must be addressed before algal biofuels can be accepted as a component of the fossil fuel replacement strategy. One significant challenge is that the cost of algal fuel production must become competitive with existing fuel alternatives. Algal biofuel production presents the opportunity to fine-tune microbial metabolic machinery for an optimal blend of biomass constituents and desired fuel molecules. Genome-scale model-driven algal metabolic design promises to facilitate both goals by directing the utilization of metabolites in the complex, interconnected metabolic networks to optimize production of the compounds of interest. Using Chlamydomonas reinhardtii as a model, we developed a systems-level methodology bridging metabolic network reconstruction with annotation and experimental verification of enzyme encoding open reading frames. We reconstructed a genome-scale metabolic network for this alga and devised a novel light-modeling approach that enables quantitative growth prediction for a given light source, resolving wavelength and photon flux. We experimentally verified transcripts accounted for in the network and physiologically validated model function through simulation and generation of new experimental growth data, providing high confidence in network contents and predictive applications. The network offers insight into algal metabolism and potential for genetic engineering and efficient light source design, a pioneering resource for studying light-driven metabolism and quantitative systems biology. Our approach to generate a predictive metabolic model integrated with cloned open reading frames, provides a cost-effective platform to generate metabolic engineering resources. While the generated resources are specific to algal systems, the approach that we have developed is not specific to algae and

  18. Transcriptional Responses to Sucrose Mimic the Plant-Associated Life Style of the Plant Growth Promoting Endophyte Enterobacter sp. 638

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Taghavi, Safiyh; Wu, Xiao; Ouyang, Liming; Zhang, Yian Biao; Stadler, Andrea; McCorkle, Sean; Zhu, Wei; Maslov, Sergei; van der Lelie, Daniel

    2015-01-21

    Growth in sucrose medium was previously found to trigger the expression of functions involved in the plant associated life style of the endophytic bacterium Enterobacter sp. 638. Therefore, comparative transcriptome analysis between cultures grown in sucrose or lactate medium was used to gain insights in the expression levels of bacterial functions involved in the endophytic life style of strain 638. Growth on sucrose as a carbon source resulted in major changes in cell physiology, including a shift from a planktonic life style to the formation of bacterial aggregates. This shift was accompanied by a decrease in transcription of genes involvedmore » in motility (e.g. flagella biosynthesis) and an increase in the transcription of genes involved in colonization, adhesion and biofilm formation. The transcription levels of functions previously suggested as being involved in endophytic behavior and functions responsible for plant growth promoting properties, including the synthesis of indole-acetic acid, acetoin and 2,3-butanediol, also increased significantly for cultures grown in sucrose medium. Interestingly, despite an abundance of essential nutrients transcription levels of functions related to uptake and processing of nitrogen and iron became increased for cultures grown on sucrose as sole carbon source. Transcriptome data were also used to analyze putative regulatory relationships. In addition to the small RNA csrABCD regulon, which seems to play a role in the physiological adaptation and possibly the shift between free-living and plant-associated endophytic life style of Enterobacter sp. 638, our results also pointed to the involvement of rcsAB in controlling responses by Enterobacter sp. 638 to a plant-associated life style. Lastly, targeted mutagenesis was used to confirm this role and showed that compared to wild-type Enterobacter sp. 638 a ΔrcsB mutant was affected in its plant growth promoting ability.« less

  19. Transcriptional Responses to Sucrose Mimic the Plant-Associated Life Style of the Plant Growth Promoting Endophyte Enterobacter sp. 638

    SciTech Connect (OSTI)

    Taghavi, Safiyh; Wu, Xiao; Ouyang, Liming; Zhang, Yian Biao; Stadler, Andrea; McCorkle, Sean; Zhu, Wei; Maslov, Sergei; van der Lelie, Daniel

    2015-01-21

    Growth in sucrose medium was previously found to trigger the expression of functions involved in the plant associated life style of the endophytic bacterium Enterobacter sp. 638. Therefore, comparative transcriptome analysis between cultures grown in sucrose or lactate medium was used to gain insights in the expression levels of bacterial functions involved in the endophytic life style of strain 638. Growth on sucrose as a carbon source resulted in major changes in cell physiology, including a shift from a planktonic life style to the formation of bacterial aggregates. This shift was accompanied by a decrease in transcription of genes involved in motility (e.g. flagella biosynthesis) and an increase in the transcription of genes involved in colonization, adhesion and biofilm formation. The transcription levels of functions previously suggested as being involved in endophytic behavior and functions responsible for plant growth promoting properties, including the synthesis of indole-acetic acid, acetoin and 2,3-butanediol, also increased significantly for cultures grown in sucrose medium. Interestingly, despite an abundance of essential nutrients transcription levels of functions related to uptake and processing of nitrogen and iron became increased for cultures grown on sucrose as sole carbon source. Transcriptome data were also used to analyze putative regulatory relationships. In addition to the small RNA csrABCD regulon, which seems to play a role in the physiological adaptation and possibly the shift between free-living and plant-associated endophytic life style of Enterobacter sp. 638, our results also pointed to the involvement of rcsAB in controlling responses by Enterobacter sp. 638 to a plant-associated life style. Lastly, targeted mutagenesis was used to confirm this role and showed that compared to wild-type Enterobacter sp. 638 a ΔrcsB mutant was affected in its plant growth promoting ability.

  20. EA-1861: DOE Notice of Availability of the Finding of No Significant...

    Broader source: Energy.gov (indexed) [DOE]

    Frito Lay Biomass Boiler Project, Beloit, Wisconsin The U.S. Department of Energy (DOE) has issued a Finding of No Significant Impact (FONSI) based on completion of an...