Sample records for find unedited transcripts

  1. UNEDITED PREPRINT Building a dynamic growth model for trembling

    E-Print Network [OSTI]

    García, Oscar

    UNEDITED PREPRINT Building a dynamic growth model for trembling aspen in Western Canada without age for even-aged thinned or unthinned stands dominated by trembling aspen. Estimation used permanent sample words: Forest growth and yield, Populus tremuloides, quacking aspen, thinning, dynamical systems, TAG. 1

  2. UNEDITED PREPUBLICATION COPY: FINAL WORDING SUBJECT TO CHANGE i BURNING PLASMA

    E-Print Network [OSTI]

    Copyright 2003 by the National Academy of Sciences. All rights reserved. Printed in the United States for the report were chosen for their special competences and with regard for appropriate balance. This study of Energy. Any opinions, findings, conclusions, or recommendations expressed in this publication are those

  3. Find Clear

    Broader source: Energy.gov (indexed) [DOE]

    p align"left">

    Find Clear Slide Title Duration Status myMail@adobe.com http:Adobe.com More Info>>

  4. Find Patents

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of Science (SC) Environmental Assessments (EA)Budget »Travel andFifthDepartmentPatents Find Patents

  5. Find Preprints

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of Science (SC) Environmental Assessments (EA)Budget »Travel andFifthDepartmentPatents Find

  6. Find Reports

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of Science (SC) Environmental Assessments (EA)Budget »Travel andFifthDepartmentPatentsReports Find

  7. Breast Cancer Research Finding Answers. Finding Cures.

    E-Print Network [OSTI]

    Kowalczykowski, Stephen C.

    Breast Cancer Research Finding Answers. Finding Cures. Thanks to improvements in treatment and early detection, more and more women are surviving breast cancer. In fact, the five-year survival rate for women with breast cancer today is 90%, up from only 63% in the 1960s. While progress has clearly been

  8. EIS-0458: Scoping Meeting Transcript

    Broader source: Energy.gov [DOE]

    Transcript of the public scoping meeting on the draft EIS for the proposed loan guarantee for the First Solar Topaz Solar Farm Project in San Luis Obispo County, CA.

  9. Analysis of transcriptional regulatory circuitry

    E-Print Network [OSTI]

    Rinaldi, Nicola J., 1974-

    2004-01-01T23:59:59.000Z

    The research in this thesis has focused on the analysis of data from two types of microarray technologies with the goal of improving understanding of transcriptional regulatory circuitry in yeast. These microarray technologies, ...

  10. Transcript for Building America Video

    Broader source: Energy.gov [DOE]

    Transcript of a video about how DOE’s Building America program is helping to bridge the gap between homes with high energy costs and homes that are healthy, durable, and energy efficient.

  11. Assessment of hepatotoxic liabilities by transcript profiling

    SciTech Connect (OSTI)

    Ruepp, Stefan [F. Hoffmann-La Roche Ltd., PRBN-S (90/5.18), CH-4070 Basel (Switzerland)]. E-mail: stefan.ruepp@roche.com; Boess, Franziska [F. Hoffmann-La Roche Ltd., PRBN-S (90/5.18), CH-4070 Basel (Switzerland); Suter, Laura [F. Hoffmann-La Roche Ltd., PRBN-S (90/5.18), CH-4070 Basel (Switzerland); Vera, Maria Cristina de [F. Hoffmann-La Roche Ltd., PRBN-S (90/5.18), CH-4070 Basel (Switzerland); Steiner, Guido [Bioinformatics, 4 CH-4070 Basel (Switzerland); Steele, Thomas [Non-Clinical Drug Safety, Nutley, NJ (United States); Weiser, Thomas [F. Hoffmann-La Roche Ltd., PRBN-S (90/5.18), CH-4070 Basel (Switzerland); Albertini, Silvio [F. Hoffmann-La Roche Ltd., PRBN-S (90/5.18), CH-4070 Basel (Switzerland)

    2005-09-01T23:59:59.000Z

    Male Wistar rats were treated with various model compounds or the appropriate vehicle controls in order to create a reference database for toxicogenomics assessment of novel compounds. Hepatotoxic compounds in the database were either known hepatotoxicants or showed hepatotoxicity during preclinical testing. Histopathology and clinical chemistry data were used to anchor the transcript profiles to an established endpoint (steatosis, cholestasis, direct acting, peroxisomal proliferation or nontoxic/control). These reference data were analyzed using a supervised learning method (support vector machines, SVM) to generate classification rules. This predictive model was subsequently used to assess compounds with regard to a potential hepatotoxic liability. A steatotic and a non-hepatotoxic 5HT{sub 6} receptor antagonist compound from the same series were successfully discriminated by this toxicogenomics model. Additionally, an example is shown where a hepatotoxic liability was correctly recognized in the absence of pathological findings. In vitro experiments and a dog study confirmed the correctness of the toxicogenomics alert. Another interesting observation was that transcript profiles indicate toxicologically relevant changes at an earlier timepoint than routinely used methods. Together, these results support the useful application of toxicogenomics in raising alerts for adverse effects and generating mechanistic hypotheses that can be followed up by confirmatory experiments.

  12. Transcription factor-based biosensor

    DOE Patents [OSTI]

    2013-10-08T23:59:59.000Z

    The present invention provides for a system comprising a BmoR transcription factor, a .sigma..sup.54-RNA polymerase, and a pBMO promoter operatively linked to a reporter gene, wherein the pBMO promoter is capable of expression of the reporter gene with an activated form of the BmoR and the .sigma..sup.54-RNA polymerase.

  13. Novel transcriptional profile in wrist muscles from cerebral palsy patients

    E-Print Network [OSTI]

    2009-01-01T23:59:59.000Z

    article Novel transcriptional profile in wrist muscles fromMethod: The transcriptional profile of spastic muscles werefirst transcriptional profile performed on spastic muscle of

  14. A Synthetic Biology Framework for Programming Eukaryotic Transcription Functions

    E-Print Network [OSTI]

    Khalil, Ahmad S.

    Eukaryotic transcription factors (TFs) perform complex and combinatorial functions within transcriptional networks. Here, we present a synthetic framework for systematically constructing eukaryotic transcription functions ...

  15. Financing Residential Energy Efficiency with Carbon Offsets Transcript...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Residential Energy Efficiency with Carbon Offsets Transcript Financing Residential Energy Efficiency with Carbon Offsets Transcript This document contains the transcript for the...

  16. Transcriptional analysis of the unicellular, diazotrophic cyanobacteri...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    analysis of the unicellular, diazotrophic cyanobacterium Cyanothece sp. ATCC 51142 grown under short daynight Transcriptional analysis of the unicellular, diazotrophic...

  17. Find People | ORNL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742EnergyOnItem NotEnergy,ARMFormsGasReleaseSpeechesHall ATours, Programs and EventsFiber LasersFind

  18. How to Find

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of Science (SC) EnvironmentalGyroSolé(tm) HarmonicbetandEnergy 2010a Wind TurbineContactFind How

  19. Find Articles/Databases

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    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of Science (SC) Environmental Assessments (EA)Budget »Travel andFifthDepartment of EnergyFind

  20. Find eBooks

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of Science (SC) Environmental Assessments (EA)Budget »TraveleBooks Find eBooks Many of these

  1. Design and characterization of artificial transcriptional terminators

    E-Print Network [OSTI]

    Huang, Haiyao

    2007-01-01T23:59:59.000Z

    Design and characterization of artificial transcriptional terminators. Ten new terminators were designed based on previous research of terminator structure and termination efficiency. The terminators were built by PCR ...

  2. Computational inference of transcriptional regulatory networks from expression proling and transcription

    E-Print Network [OSTI]

    Babu, M. Madan

    Bioinformatics Program, 2 Department of Biology and 3 Biomedical Engineering Department, Boston University, 44Computational inference of transcriptional regulatory networks from expression pro ABSTRACT We have developed a computational method for transcriptional regulatory network inference, CARRIE

  3. TO APPEAR IN: Z MAGAZINE, JUNE 2006. (UNEDITED VERSION)

    E-Print Network [OSTI]

    O'Donnell, Tom

    sufficient low-level enriched uranium to use in fuel rods for their Russian-built nuclear power plant. But threat" posed by Iran. Rather, the American push against Iran's nuclear program and for "regime change is out to remove the mullahs from power. Just this February, Bush was very pleased to recognize India

  4. How do I get my training transcripts? 1. HR Training Transcript

    E-Print Network [OSTI]

    Howitt, Ivan

    How do I get my training transcripts? 1. HR Training Transcript There are two ways to access your HR Training Transcript ­ either through Banner Self Service or 49er Express. I) Using Banner Self credentials, then select the "Employee" tab Select the "Human Resources Training Courses Completed" link II

  5. MOPAC: motif finding by preprocessing and agglomerative clustering from microarrays

    E-Print Network [OSTI]

    Rajagopalan, Ganesh

    2002-01-01T23:59:59.000Z

    controlled is by gene regulation via Regulatory protein Basal transcription factors mRNA Motif Promoter region Upstream sequence ORP / Gene Transcription Start Figure 1. 1. A snapshot of transcription initiation transcriptional control (Figure 1. 1...

  6. Placental findings in cord accidents

    E-Print Network [OSTI]

    Parast, Mana M

    2012-01-01T23:59:59.000Z

    Placental findings in cord accidents. BMC Pregnancy andPlacental findings in cord accidents Mana M Parast Fromfor stillbirth. “Cord accident,” defined by obstruction of

  7. Transcriptional regulation constrains the organization of genes on eukaryotic chromosomes

    E-Print Network [OSTI]

    Babu, M. Madan

    Transcriptional regulation constrains the organization of genes on eukaryotic chromosomes Sarath. Although it is known that eukaryotic transcriptional regulation is complex and requires an intricate-order organization of genes across and within chromosomes that is constrained by transcriptional regulation

  8. Evolution of transcription networks in response to temporal fluctuations

    E-Print Network [OSTI]

    Hespanha, Joăo Pedro

    Evolution of transcription networks in response to temporal fluctuations Journal: Evolution, Evolution & Marine Biology Keywords: Population Genetics, Epistasis, Genetic Networks, Transcription Evolution: For Review Only #12;EVOLUTION OF TRANSCRIPTION NETWORKS IN RESPONSE TO TEMPORAL FLUCTUATIONS

  9. The Role of Multiple Transcription Factors In Archaeal Gene Expression

    SciTech Connect (OSTI)

    Charles J. Daniels

    2008-09-23T23:59:59.000Z

    Since the inception of this research program, the project has focused on two central questions: What is the relationship between the 'eukaryal-like' transcription machinery of archaeal cells and its counterparts in eukaryal cells? And, how does the archaeal cell control gene expression using its mosaic of eukaryal core transcription machinery and its bacterial-like transcription regulatory proteins? During the grant period we have addressed these questions using a variety of in vivo approaches and have sought to specifically define the roles of the multiple TATA binding protein (TBP) and TFIIB-like (TFB) proteins in controlling gene expression in Haloferax volcanii. H. volcanii was initially chosen as a model for the Archaea based on the availability of suitable genetic tools; however, later studies showed that all haloarchaea possessed multiple tbp and tfb genes, which led to the proposal that multiple TBP and TFB proteins may function in a manner similar to alternative sigma factors in bacterial cells. In vivo transcription and promoter analysis established a clear relationship between the promoter requirements of haloarchaeal genes and those of the eukaryal RNA polymerase II promoter. Studies on heat shock gene promoters, and the demonstration that specific tfb genes were induced by heat shock, provided the first indication that TFB proteins may direct expression of specific gene families. The construction of strains lacking tbp or tfb genes, coupled with the finding that many of these genes are differentially expressed under varying growth conditions, provided further support for this model. Genetic tools were also developed that led to the construction of insertion and deletion mutants, and a novel gene expression scheme was designed that allowed the controlled expression of these genes in vivo. More recent studies have used a whole genome array to examine the expression of these genes and we have established a linkage between the expression of specific tfb genes and the regulation of nitrogen metabolism and other global cellular responses.

  10. Jet finding techniques at LHC

    E-Print Network [OSTI]

    BOUMEDIENE, D; The ATLAS collaboration

    2015-01-01T23:59:59.000Z

    Jet finding techniques at hadron colliders, including pile-up removal tricks, jet deconstruction, etc

  11. Dual spatial maps of transcript and protein abundance in the...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Dual spatial maps of transcript and protein abundance in the mouse brain. Dual spatial maps of transcript and protein abundance in the mouse brain. Abstract: Integrating...

  12. Transcript of See Action and Technical Assistance Program Webcast...

    Broader source: Energy.gov (indexed) [DOE]

    Transcript of See Action and Technical Assistance Program Webcast: Energy Audit and Retro-Commissioning Policies for Public and Commercial Buildings Transcript of See Action and...

  13. AcademiCast Transcript Texas Tech University

    E-Print Network [OSTI]

    Rock, Chris

    West Texas' quail population. The region's quail numbers plummeted in 2010 in West Texas and western Oklahoma. So far, scientists have found lead, mercuryAcademiCast Transcript Texas Tech University August 30, 2013 Pierce

  14. Control of Transcription by Cell Size

    E-Print Network [OSTI]

    Wu, Chia-Yung

    Cell size increases significantly with increasing ploidy. Differences in cell size and ploidy are associated with alterations in gene expression, although no direct connection has been made between cell size and transcription. ...

  15. TAP Webcast Transcript July-29, 2009

    Broader source: Energy.gov [DOE]

    Transcript of the audio for the July 2009 TAP Webcast titled Economic Impacts of Renewable Energy sponsored by the U.S. Department of Energy (DOE) Technical Assistance Project (TAP) for state and local officials.

  16. Finding

    Office of Legacy Management (LM)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742EnergyOn AprilA groupTuba City, Arizona, DisposalFourthNrr-osams ADMIN551 - g 7 s % @ {rField

  17. Multi-level Modeling and Inference of Transcription Regulation

    E-Print Network [OSTI]

    Shamir, Ron

    promoters and the combinatorial regulation of transcription. At the core, our model manipulates doseMulti-level Modeling and Inference of Transcription Regulation Amos Tanay and Ron Shamir School to infer mechanistic models of transcription control. We propose a new model which integrates transcription

  18. Transcription factor-based biosensors for detecting dicarboxylic acids

    DOE Patents [OSTI]

    Dietrich, Jeffrey; Keasling, Jay

    2014-02-18T23:59:59.000Z

    The invention provides methods and compositions for detecting dicarboxylic acids using a transcription factor biosensor.

  19. Nonspecific transcription factor binding reduces variability in transcription factor and target protein expression

    E-Print Network [OSTI]

    Mohammad Soltani; Pavol Bokes; Zachary Fox; Abhyudai Singh

    2015-04-14T23:59:59.000Z

    Transcription factors (TFs) interact with a multitude of binding sites on DNA and partner proteins inside cells. We investigate how nonspecific binding/unbinding to such decoy binding sites affects the magnitude and time-scale of random fluctuations in TF copy numbers arising from stochastic gene expression. A stochastic model of TF gene expression, together with decoy site interactions is formulated. Distributions for the total (bound and unbound) and free (unbound) TF levels are derived by analytically solving the chemical master equation under physiologically relevant assumptions. Our results show that increasing the number of decoy binding sides considerably reduces stochasticity in free TF copy numbers. The TF autocorrelation function reveals that decoy sites can either enhance or shorten the time-scale of TF fluctuations depending on model parameters. To understand how noise in TF abundances propagates downstream, a TF target gene is included in the model. Intriguingly, we find that noise in the expression of the target gene decreases with increasing decoy sites for linear TF-target protein dose-responses, even in regimes where decoy sites enhance TF autocorrelation times. Moreover, counterintuitive noise transmissions arise for nonlinear dose-responses. In summary, our study highlights the critical role of molecular sequestration by decoy binding sites in regulating the stochastic dynamics of TFs and target proteins at the single-cell level.

  20. Finding a Job in Mathematics

    E-Print Network [OSTI]

    Below we have attempted to collect a number of links to information on finding jobs ... (Purdue); The National Security Agency is reputed to be the world's largest ...

  1. Facilitated diffusion framework for transcription factor search with conformational changes

    E-Print Network [OSTI]

    Jérôme Cartailler; Jürgen Reingruber

    2015-07-09T23:59:59.000Z

    Cellular responses often require the fast activation or repression of specific genes, which depends on Transcription Factors (TFs) that have to quickly find the promoters of these genes within a large genome. Transcription Factors (TFs) search for their DNA promoter target by alternating between bulk diffusion and sliding along the DNA, a mechanism known as facilitated diffusion. We study a facilitated diffusion framework with switching between three search modes: a bulk mode and two sliding modes triggered by conformational changes between two protein conformations. In one conformation (search mode) the TF interacts unspecifically with the DNA backbone resulting in fast sliding. In the other conformation (recognition mode) it interacts specifically and strongly with DNA base pairs leading to slow displacement. From the bulk, a TF associates with the DNA at a random position that is correlated with the previous dissociation point, which implicitly is a function of the DNA structure. The target affinity depends on the conformation. We derive exact expressions for the mean first passage time (MFPT) to bind to the promoter and the conditional probability to bind before detaching when arriving at the promoter site. We systematically explore the parameter space and compare various search scenarios. We compare our results with experimental data for the dimeric Lac repressor search in E.Coli bacteria. We find that a coiled DNA conformation is absolutely necessary for a fast MFPT. With frequent spontaneous conformational changes, a fast search time is achieved even when a TF becomes immobilized in the recognition state due to the specific bindings. We find a MFPT compatible with experimental data in presence of a specific TF-DNA interaction energy that has a Gaussian distribution with a large variance.

  2. Method to determine transcriptional regulation pathways in organisms

    DOE Patents [OSTI]

    Gardner, Timothy S.; Collins, James J.; Hayete, Boris; Faith, Jeremiah

    2012-11-06T23:59:59.000Z

    The invention relates to computer-implemented methods and systems for identifying regulatory relationships between expressed regulating polypeptides and targets of the regulatory activities of such regulating polypeptides. More specifically, the invention provides a new method for identifying regulatory dependencies between biochemical species in a cell. In particular embodiments, provided are computer-implemented methods for identifying a regulatory interaction between a transcription factor and a gene target of the transcription factor, or between a transcription factor and a set of gene targets of the transcription factor. Further provided are genome-scale methods for predicting regulatory interactions between a set of transcription factors and a corresponding set of transcriptional target substrates thereof.

  3. Finding beam focus errors automatically

    SciTech Connect (OSTI)

    Lee, M.J.; Clearwater, S.H.; Kleban, S.D.

    1987-01-01T23:59:59.000Z

    An automated method for finding beam focus errors using an optimization program called COMFORT-PLUS. The steps involved in finding the correction factors using COMFORT-PLUS has been used to find the beam focus errors for two damping rings at the SLAC Linear Collider. The program is to be used as an off-line program to analyze actual measured data for any SLC system. A limitation on the application of this procedure is found to be that it depends on the magnitude of the machine errors. Another is that the program is not totally automated since the user must decide a priori where to look for errors. (LEW)

  4. AcademiCast Transcript Texas Tech University

    E-Print Network [OSTI]

    Rock, Chris

    and Research in the College of Education, is the premier center in West TexasAcademiCast Transcript Texas Tech University December 12, 2012 Irlbeck: This is AcademiCast--Texas Tech University's podcast series from the Office of the Provost

  5. TRANSCRIPT OF RECORDS Mr / Mrs STEVENS, Alex

    E-Print Network [OSTI]

    Rossak, Wilhelm R.

    TRANSCRIPT OF RECORDS Mr / Mrs STEVENS, Alex born on 08.08.1988 student of Ghent University type1 Course title Assess ment2 Contact hours/ week Contact hours in total Instructor3 Local Grade4 ECTS Grade5 ECTS Credits6 36612 S Berufssprache Deutsch (1/2) [BA.DaF.ATS.05] -- 4 60 Nailia Mukmenova

  6. Findings in seal coat design

    E-Print Network [OSTI]

    Gonzalez Palmer, Miguel Angel

    1988-01-01T23:59:59.000Z

    FINDINGS IN SEAL COAT DESIGN A Thesis by MIGUEL ANGEL GONZALEZ PALMER Submitted to the Graduate College of Texas A&M University in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE May 1988 Major Subject : Civil... Engineering FINDINGS IN SEAL COAT DESIGN A Thesis by MIGUEL ANGEL GONZALEZ PALMER Approved as to style and content by: Michael P. J. Olsen (Chairman of Committee) Dallas N. Little (Member) ne D. Tiner (Member) James T. P. Yao (Head of Departme...

  7. Transcriptional Regulation of Pregnane X Receptor by Protein Arginine Methyltransferase 

    E-Print Network [OSTI]

    Xie, Ying

    2011-08-08T23:59:59.000Z

    Pregnane X receptor (PXR) is a ligand-dependent transcription factor that plays an important role in xenobiotic/drug metabolism. The ligand-receptor interaction transcriptionally activates phase I and phase II enzymes, and membrane...

  8. Genome-Wide In Silico Identification of Transcriptional Regulators Controlling

    E-Print Network [OSTI]

    Shamir, Ron

    Genome-Wide In Silico Identification of Transcriptional Regulators Controlling the Cell Cycle networks that control gene transcription is one of the greatest challenges of functional genomics. Using human genomic sequences, models for binding sites of known transcription factors, and gene expression

  9. Peak finding using biorthogonal wavelets

    SciTech Connect (OSTI)

    Tan, C.Y.

    2000-02-01T23:59:59.000Z

    The authors show in this paper how they can find the peaks in the input data if the underlying signal is a sum of Lorentzians. In order to project the data into a space of Lorentzian like functions, they show explicitly the construction of scaling functions which look like Lorentzians. From this construction, they can calculate the biorthogonal filter coefficients for both the analysis and synthesis functions. They then compare their biorthogonal wavelets to the FBI (Federal Bureau of Investigations) wavelets when used for peak finding in noisy data. They will show that in this instance, their filters perform much better than the FBI wavelets.

  10. Diffusion, dimensionality and noise in transcriptional regulation

    E-Print Network [OSTI]

    Gasper Tkacik; William Bialek

    2007-12-12T23:59:59.000Z

    The precision of biochemical signaling is limited by randomness in the diffusive arrival of molecules at their targets. For proteins binding to the specific sites on the DNA and regulating transcription, the ability of the proteins to diffuse in one dimension by sliding along the length of the DNA, in addition to their diffusion in bulk solution, would seem to generate a larger target for DNA binding, consequently reducing the noise in the occupancy of the regulatory site. Here we show that this effect is largely cancelled by the enhanced temporal correlations in one dimensional diffusion. With realistic parameters, sliding along DNA has surprisingly little effect on the physical limits to the precision of transcriptional regulation.

  11. Analysis of transcriptional responses in the mouse dorsal striatum following acute MDMA (Ecstasy): identification of ERK-controlled genes.

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    ): identification of ERK-controlled genes. J. Salzmann, C. Canestrelli, F. Noble*, and C. Marie-Claire. Université rewarding effects in mice were dependent upon ERK activation and that dorsal striatum was a critical region for mediating ERK-dependent Egr1 MDMA-induced transcription. Here, we extend these findings by showing that MDMA

  12. Theoretical analysis of transcription process with polymerase stalling

    E-Print Network [OSTI]

    Jingwei Li; Yunxin Zhang

    2015-02-07T23:59:59.000Z

    Experimental evidences show that in gene transcription, RNA polymerase has the possibility to be stalled at certain position of the transcription template. This may be due to the template damage, or protein barriers. Once stalled, polymerase may backtrack along the template to the previous nucleotide to wait for the repair of the damaged site, or simply bypass the barrier or damaged site and consequently synthesize an incorrect messenger RNA, or degrade and detach from the template. Thus, the {\\it effective} transcription rate (the rate to synthesize correct product mRNA) and the transcription {\\it effectiveness} (the ratio of the {\\it effective} transcription rate to the {\\it effective} transcription initiation rate) are both influenced by polymerase stalling events. This study shows that, Without backtracking, detachment of stalled polymerase can also help to increase the {\\it effective} transcription rate and transcription {\\it effectiveness}. Generally, the increase of bypass rate of the stalled polymerase will lead to the decrease of the {\\it effective} transcription rate and transcription {\\it effectiveness}. But when both detachment rate and backtracking rate of the stalled polymerase vanish, the {\\it effective} transcription rate may also be increased by the bypass mechanism.

  13. The Transcription Unit Architecture of the Escherichia Coli Genome

    SciTech Connect (OSTI)

    Cho, Byung-Kwan; Zengler, Karsten; Qiu, Yu; Park, Young S.; Knight, Eric M.; Barrett, Christian; Gao, Yuan; Palsson, Bernhard O.

    2009-11-30T23:59:59.000Z

    Under EMSL User Proposal 25660, the authors reported that bacterial genomes are organized by structural and functional elements, including promoters, transcription start and termination sites, open reading frames, regulatory noncoding regions, untranslated regions and transcription units. Here, we iteratively integrate high-throughput, genome-wide measurements of RNA polymerase binding locations and mRNA transcript abundance, 5? sequences and translation into proteins to determine the organizational structure of the Escherichia coli K-12 MG1655 genome. Integration of the organizational elements provides an experimentally annotated transcription unit architecture, including alternative transcription start sites, 5? untranslated region, boundaries and open reading frames of each transcription unit. A total of 4,661 transcription units were identified, representing an increase of >530% over current knowledge. This comprehensive transcription unit architecture allows for the elucidation of condition-specific uses of alternative sigma factors at the genome scale. Furthermore, the transcription unit architecture provides a foundation on which to construct genome-scale transcriptional and translational regulatory networks.

  14. Find Internships | Department of Energy

    Office of Environmental Management (EM)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742 33 1112011 Strategic Plan| Departmentof Ohio EnvironmentalEnergyFinancing CleanInternships Find

  15. Protein synthesis driven by dynamical stochastic transcription

    E-Print Network [OSTI]

    Guilherme C. P. Innocentini; Michael Forger; Fernando Antoneli

    2014-12-23T23:59:59.000Z

    In this letter we propose a mathematical framework to couple transcription and translation in which mRNA production is described by a set of master equations while the dynamics of protein density is governed by a random differential equation. The coupling between the two processes is given by a stochastic perturbation whose statistics satisfies the master equations. In this approach, from the knowledge of the analytical time dependent distribution of mRNA number, we are able to calculate the dynamics of the probability density of the protein population.

  16. Binding of transcription factors adapts to resolve information-energy trade-off

    E-Print Network [OSTI]

    Kagan, Yonatan Savir Jacob

    2015-01-01T23:59:59.000Z

    We examine the binding of transcription factors to DNA in terms of an information transfer problem. The input of the noisy channel is the biophysical signal of a factor bound to a DNA site, and the output is a distribution of probable DNA sequences at this site. This task involves an inherent tradeoff between the information gain and the energetics of the binding interaction - high binding energies provide higher information gain but hinder the dynamics of the system as factors are bound too tightly. We show that adaptation of the binding interaction towards increasing information transfer under energy constraints implies that the information gain per specific binding energy at each base-pair is maximized. We analyze hundreds of prokaryote and eukaryote transcription factors from various organisms to evaluate the discrimination energies. We find that, in accordance with our theoretical argument, binding energies nearly maximize the information gain per energy. This work suggests the adaptation of information ...

  17. transcript_jedi_model.doc | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    transcriptjedimodel.doc More Documents & Publications TAP Webcast Transcript July-29, 2009 Scenario Jedi Tools for Designing & Implementing Better Finance Programs...

  18. Partnering With Utilities to Offer Energy Efficiency Programs Webinar Transcript

    Broader source: Energy.gov [DOE]

    Partnering With Utilities to Offer Energy Efficiency Programs Webinar Transcript, from the U.S. Department of Energy Technical Assistance Program (TAP).

  19. Blackout 2003: Transcript of Technical Conference with the Electric...

    Office of Environmental Management (EM)

    consultation Transcript of the technical conference sponsored by the U.S. Canada task force investigating the August 14, 2003 blackout across Canada and the...

  20. Transcript, Keeping the Edge: Enhancing Performance by Managing Culture

    Broader source: Energy.gov [DOE]

    Transcript: 2003 INPO CEO Conference, Keeping the Edge: “Enhancing Performance by Managing Culture” Edgar H. Schein, Ph.D. Professor Emeritis, Massachusetts Institute of Technology November 7, 2003

  1. EIS-0429: Scoping Meeting Transcript, 12/3/2009 | Department...

    Broader source: Energy.gov (indexed) [DOE]

    1232009 EIS-0429: Scoping Meeting Transcript, 1232009 Indiana Gasification, LLC, Industrial Gasification Facility in Rockport, IN and CO2 Pipeline Public Scoping Meeting - 12...

  2. BetterBuildings Webinar Transcription - Financial Vehicles within...

    Energy Savers [EERE]

    Financial Institution Partners Transcript.doc Better Buildings Neighborhood Program Home Accomplishments History Better Buildings Partners Stories Interviews Videos Contact Us...

  3. arabidopsis bzip1 transcription: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    spring Lee, Ilha 2 Versatile Gene-Specific Sequence Tags for Arabidopsis Functional Genomics: Transcript Profiling Biology and Medicine Websites Summary: VIB MicroArray Facility,...

  4. arabidopsis myb5 transcription: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    spring Lee, Ilha 2 Versatile Gene-Specific Sequence Tags for Arabidopsis Functional Genomics: Transcript Profiling Biology and Medicine Websites Summary: VIB MicroArray Facility,...

  5. arabidopsis wrky2 transcription: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    spring Lee, Ilha 2 Versatile Gene-Specific Sequence Tags for Arabidopsis Functional Genomics: Transcript Profiling Biology and Medicine Websites Summary: VIB MicroArray Facility,...

  6. Transcriptional and translational regulatory responses to iron...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of Candidatus Pelagibacter ubique cultures to iron limitation in natural seawater media supplemented with a siderophore to chelate iron. MethodologyPrincipal Findings:...

  7. Regional Transmission Projects: Finding Solutions

    SciTech Connect (OSTI)

    The Keystone Center

    2005-06-15T23:59:59.000Z

    The Keystone Center convened and facilitated a year-long Dialogue on "Regional Transmission Projects: Finding Solutions" to develop recommendations that will help address the difficult and contentious issues related to expansions of regional electric transmission systems that are needed for reliable and economic transmission of power within and across regions. This effort brought together a cross-section of affected stakeholders and thought leaders to address the problem with the collective wisdom of their experience and interests. Transmission owners sat at the table with consumer advocates and environmental organizations. Representatives from regional transmission organizations exchanged ideas with state and federal regulators. Generation developers explored common interests with public power suppliers. Together, the Dialogue participants developed consensus solutions about how to begin unraveling some of the more intractable issues surrounding identification of need, allocation of costs, and reaching consensus on siting issues that can frustrate the development of regional transmission infrastructure. The recommendations fall into three broad categories: 1. Recommendations on appropriate institutional arrangements and processes for achieving regional consensus on the need for new or expanded transmission infrastructure 2. Recommendations on the process for siting of transmission lines 3. Recommendations on the tools needed to support regional planning, cost allocation, and siting efforts. List of Dialogue participants: List of Dialogue Participants: American Electric Power American Transmission Company American Wind Energy Association California ISO Calpine Corporation Cinergy Edison Electric Institute Environmental Defense Federal Energy Regulatory Commission Great River Energy International Transmission Company ISO-New England Iowa Public Utility Board Kanner & Associates Midwest ISO National Association of Regulatory Utility Commissioners National Association of State Utility Consumer Advocates National Grid Northeast Utilities PA Office of Consumer Advocates Pacific Gas & Electric Corporation Pennsylvania Public Utility Commission PJM Interconnection The Electricity Consumers Resource Council U.S. Department of Energy US Department of the Interior Van Ness Feldman Western Interstate Energy Board Wind on the Wires Wisconsin Public Service Commission Xcel Energy

  8. Finding & Sharing Information about Energy Efficiency | Department...

    Broader source: Energy.gov (indexed) [DOE]

    Finding & Sharing Information about Energy Efficiency Finding & Sharing Information about Energy Efficiency June 29, 2011 - 12:27pm Addthis Drew Bittner Communications Lead, Office...

  9. Transients in chloroplast gene transcription Sujith Puthiyaveetil, John F. Allen *

    E-Print Network [OSTI]

    Allen, John F.

    of the reaction centres of photosystem I and photosystem II. Their transcription is regulated by changes in wave protein"). The changes in transcript abundance in the model plant Arabidopsis thali- ana were initiated recognized for its wide linear dynamic range of detection, high sensi- tivity and reproducibility over

  10. Transcriptional Regulation in Algae, Fungi and Plants: Mating Loci, Splicing, and miRNAs

    E-Print Network [OSTI]

    Douglass, Stephen Michael

    2014-01-01T23:59:59.000Z

    from organisms ranging from algae to fungi and plants. WeTranscriptional Regulation in Algae, Fungi and Plants:Transcriptional Regulation in Algae, Fungi and Plants:

  11. SUMOylation of ROR{alpha} potentiates transcriptional activation function

    SciTech Connect (OSTI)

    Hwang, Eun Ju [Department of Biological Sciences, Research Center for Women's Disease, Sookmyung Women's University, 52 Hyochangwon-gil, Yongsan-gu, Seoul 140-742 (Korea, Republic of); Lee, Ji Min [Department of Biological Sciences, Seoul National University, Seoul 151-742 (Korea, Republic of); Jeong, Jiyeong; Park, Joo Hyeon; Yang, Young; Lim, Jong-Seok [Department of Biological Sciences, Research Center for Women's Disease, Sookmyung Women's University, 52 Hyochangwon-gil, Yongsan-gu, Seoul 140-742 (Korea, Republic of); Kim, Jung Hwa [Department of Medical Sciences, Inha University, Incheon 402-751 (Korea, Republic of); Baek, Sung Hee [Department of Biological Sciences, Seoul National University, Seoul 151-742 (Korea, Republic of); Kim, Keun Il [Department of Biological Sciences, Research Center for Women's Disease, Sookmyung Women's University, 52 Hyochangwon-gil, Yongsan-gu, Seoul 140-742 (Korea, Republic of)], E-mail: kikim@sookmyung.ac.kr

    2009-01-16T23:59:59.000Z

    SUMOylation regulates a variety of cellular processes, including control of transcriptional activities of nuclear receptors. Here, we present SUMOylation of orphan nuclear receptor, ROR{alpha} by both SUMO-1 and SUMO-2. SUMOylation of ROR{alpha} occurred on the 240th lysine residue at the hinge region of human protein. PIAS family members, PIASx{alpha}, PIAS3, and PIASy, increased SUMOylation of ROR{alpha}, whereas SENP2 specifically removed SUMO from ROR{alpha}. SUMOylation-defective mutant form of ROR{alpha} exhibited decreased transcriptional activity on ROR{alpha}-responsive promoters indicating that SUMOylation may positively regulate transcriptional function of ROR{alpha}.

  12. MFR PAPER 1230 Finding Fish With Satellites

    E-Print Network [OSTI]

    sensors, fishing vessels , spotter pilots, research vessels, and offshore oil platforms were used Investigation? A. It is an attempt to find out if satellites can help fishermen find fish. Our assumption

  13. activated transcription factors: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Vijaya L. Krishnan; Ian M. Adcock; Peter J. Barnes; K. Fan Chung 1998-01-01 4 The Turkey Transcription Factor Pit-1GHF-1 Can Activate the Turkey Prolactin and Growth Hormone...

  14. RESEARCH Open Access Cellular transcriptional profiling in human lung

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    RESEARCH Open Access Cellular transcriptional profiling in human lung epithelial cells infected expression profiles of human lung A549 cells infected by five different subtypes of human and avian influenza

  15. Transcriptional Regulation by CHIP/LDB Complexes Revital Bronstein1

    E-Print Network [OSTI]

    Shamir, Ron

    Segal1 * 1 Department of Microbiology and Biotechnology, Tel Aviv University, Tel Aviv, Israel, 2 to the study of transcription regulation of gene batteries required for development. Citation: Bronstein R

  16. Transcriptional regulation of taxol™ biosynthesis in taxus cuspidate procambium cells 

    E-Print Network [OSTI]

    Waibel, Thomas

    2011-11-24T23:59:59.000Z

    This thesis presents an investigation into the transcriptional regulation of TaxolTM biosynthsis in Taxus cuspidata cell suspension cultures. The potent anticancer drug TaxolTM has been shown to be successful in the ...

  17. The AMI System for the Transcription of Speech in Meetings 

    E-Print Network [OSTI]

    Hain, Thomas; Burget, Lukas; Dines, John; Garau, Giulia; Wan, Vincent; Karafiat, Martin; Vepa, Jithendra; Lincoln, Michael

    2007-01-01T23:59:59.000Z

    This paper describes the AMI transcription system for speech in meetings developed in collaboration by five research groups. The system includes generic techniques such as discriminative and speaker adaptive training, ...

  18. Densely Interconnected Transcriptional Circuits Control Cell States in Human Hematopoiesis

    E-Print Network [OSTI]

    Novershtern, Noa

    Though many individual transcription factors are known to regulate hematopoietic differentiation, major aspects of the global architecture of hematopoiesis remain unknown. Here, we profiled gene expression in 38 distinct ...

  19. Dissecting the spatial structure of overlapping transcription in budding yeast

    E-Print Network [OSTI]

    Danford, Timothy W. (Timothy William), 1979-

    2010-01-01T23:59:59.000Z

    This thesis presents a computational and algorithmic method for the analysis of high-resolution transcription data in the budding yeast Saccharomyces cerevisiae. We begin by describing a computational system for storing ...

  20. Multispecies diel transcriptional oscillations in open ocean heterotrophic bacterial assemblages

    E-Print Network [OSTI]

    Marin, R.

    Oscillating diurnal rhythms of gene transcription, metabolic activity, and behavior are found in all three domains of life. However, diel cycles in naturally occurring heterotrophic bacteria and archaea have rarely been ...

  1. arabidopsis bzip transcription: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    13 14 15 16 17 18 19 20 21 22 23 24 25 Next Page Last Page Topic Index 1 Functional genomics analysis of the arabidopsis ABI5 bZIP transcription factor Texas A&M University -...

  2. Androgen Receptor Repression of GnRH Gene Transcription

    E-Print Network [OSTI]

    Mellon, Pamela L.

    , and progesterone receptor (PR)A (4­6). The AR is a ligand-activated transcription factor, a member of the nuclear November 10, 2011 Abbreviations: AR, Androgen receptor; ChIP, chromatin immunoprecipitation; cs, char- coal

  3. TRES predicts transcription control in embryonic stem cells.

    E-Print Network [OSTI]

    Pooley, Christopher; Ruau, David; Lombard, Patrick; Gottgens, Berthold; Joshi, Anagha

    2014-06-23T23:59:59.000Z

    have developed a web tool called ‘Transcription Regulation in Embyonic Stem Cells’, or TRES for short, to link gene sets to likely upstream regulators in ES cells. *To whom correspondence should be addressed. 2 THE TRES PIPELINE Figure 1A... our understanding of normal development as well as disease. To facili- tate this, we have developed a novel web tool called ‘TRES’ that predicts the likely upstream regulators for a given gene list. This is achieved by integrating transcription...

  4. Transcription Factors Bind Thousands of Active and InactiveRegions in the Drosophila Blastoderm

    SciTech Connect (OSTI)

    Li, Xiao-Yong; MacArthur, Stewart; Bourgon, Richard; Nix, David; Pollard, Daniel A.; Iyer, Venky N.; Hechmer, Aaron; Simirenko, Lisa; Stapleton, Mark; Luengo Hendriks, Cris L.; Chu, Hou Cheng; Ogawa, Nobuo; Inwood, William; Sementchenko, Victor; Beaton, Amy; Weiszmann, Richard; Celniker, Susan E.; Knowles, David W.; Gingeras, Tom; Speed, Terence P.; Eisen, Michael B.; Biggin, Mark D.

    2008-01-10T23:59:59.000Z

    Identifying the genomic regions bound by sequence-specific regulatory factors is central both to deciphering the complex DNA cis-regulatory code that controls transcription in metazoans and to determining the range of genes that shape animal morphogenesis. Here, we use whole-genome tiling arrays to map sequences bound in Drosophila melanogaster embryos by the six maternal and gap transcription factors that initiate anterior-posterior patterning. We find that these sequence-specific DNA binding proteins bind with quantitatively different specificities to highly overlapping sets of several thousand genomic regions in blastoderm embryos. Specific high- and moderate-affinity in vitro recognition sequences for each factor are enriched in bound regions. This enrichment, however, is not sufficient to explain the pattern of binding in vivo and varies in a context-dependent manner, demonstrating that higher-order rules must govern targeting of transcription factors. The more highly bound regions include all of the over forty well-characterized enhancers known to respond to these factors as well as several hundred putative new cis-regulatory modules clustered near developmental regulators and other genes with patterned expression at this stage of embryogenesis. The new targets include most of the microRNAs (miRNAs) transcribed in the blastoderm, as well as all major zygotically transcribed dorsal-ventral patterning genes, whose expression we show to be quantitatively modulated by anterior-posterior factors. In addition to these highly bound regions, there are several thousand regions that are reproducibly bound at lower levels. However, these poorly bound regions are, collectively, far more distant from genes transcribed in the blastoderm than highly bound regions; are preferentially found in protein-coding sequences; and are less conserved than highly bound regions. Together these observations suggest that many of these poorly-bound regions are not involved in early-embryonic transcriptional regulation, and a significant proportion may be nonfunctional. Surprisingly, for five of the six factors, their recognition sites are not unambiguously more constrained evolutionarily than the immediate flanking DNA, even in more highly bound and presumably functional regions, indicating that comparative DNA sequence analysis is limited in its ability to identify functional transcription factor targets.

  5. Transcript of the Guidelines for Home Energy Professionals project Prezi presentation.

    Broader source: Energy.gov [DOE]

    This document contains the transcript of the Guidelines for Home Energy Professionals project Prezi presentation.

  6. Energy Savings Performance Contracting-Savings Measurement and Verification Transcript 2-24-2011.doc

    Broader source: Energy.gov [DOE]

    Energy Savings Performance Contracting-Savings Measurement and Verification Transcript 2-24-2011.doc

  7. Sustainable Energy Resources for Consumers Webinar on Solar Water Heating Transcript

    Broader source: Energy.gov [DOE]

    Video recording transcript of a Webinar on Nov. 16, 2010 about residential solar water heating applications

  8. Community Development Finance Institutions-Opportunities for Partnerships with Energy Efficiency Programs Transcript.doc

    Broader source: Energy.gov [DOE]

    Community Development Finance Institutions-Opportunities for Partnerships with Energy Efficiency Programs Transcript.doc

  9. Application for the Issue of a Replacement Transcript To apply for the issue of a replacement transcript, complete all relevant sections below and

    E-Print Network [OSTI]

    Neirotti, Juan Pablo

    Page | 1 Application for the Issue of a Replacement Transcript To apply for the issue of a replacement transcript, complete all relevant sections below and return this form to Examinations students You will not be charged for this service. A replacement transcript can be supplied

  10. Beyond Academia: How to Find a Job

    E-Print Network [OSTI]

    Kaminsky, Werner

    Beyond Academia: How to Find a Job A critical piece of the job search process is knowing where and how to search for positions. This handout describes many different ways to find jobs outside academia by the job-hunter, asking for proof of what the job- hunter can do Contacts: Using a friend or business

  11. Suppression and enhancement of transcriptional noise by DNA looping

    E-Print Network [OSTI]

    Jose M. G. Vilar; Leonor Saiz

    2014-06-11T23:59:59.000Z

    DNA looping has been observed to enhance and suppress transcriptional noise but it is uncertain which of these two opposite effects is to be expected for given conditions. Here, we derive analytical expressions for the main quantifiers of transcriptional noise in terms of the molecular parameters and elucidate the role of DNA looping. Our results rationalize paradoxical experimental observations and provide the first quantitative explanation of landmark individual-cell measurements at the single molecule level on the classical lac operon genetic system [Choi et al., Science 322, 442-446 (2008)].

  12. Persistent search trees and maxima finding

    E-Print Network [OSTI]

    Collins, Carol Esther

    1987-01-01T23:59:59.000Z

    PERSISTENT SEARCH TREES AND MAXIMA FINDING A Thesis by CAROL ESTHER COLLINS Submitted to the Graduate College of Texas A%M University in partial fulfillment of the requirement for the degree of MASTER OF SCIENCE August 1987 Major Subject...: Computer Science PERSISTENT SEARCH TREES AND MAXIMA FINDING A Thesis by CAROL ESTHER COLLINS Approved as to style and content by: Donald K. Friesen (Chairman of Committee) David Fol y (Member Bryan Deuermeye (Member) G. illiams (Head of Departm...

  13. Mechanism Based Anticancer Drugs that Degrade Sp Transcription Factors

    E-Print Network [OSTI]

    Chadalapaka, Gayathri

    2013-03-14T23:59:59.000Z

    4 transcription factors. Similar results were observed for a structurally-related triterpenoid, methyl 2-cyano-3,12-dioxooleana-1,9-dien-28-oate (CDDO-Me), which is currently in clinical trials for treatment of leukemia. Celastrol, a naturally...

  14. Interactive Media Research & Speech Recognition: usable transcriptions of webcast lectures

    E-Print Network [OSTI]

    Penn, Gerald

    Modelling for Automatic Lecture Transcription. In Proceedings of the Tenth European Conference on Speech. In Proceedings of the ACM SIGCHI Conference on Human Factors in Computing Systems - CHI ­ Workshop on Usable When it Isn't. In Proceedings of the Eight International Conference on Multimodal Interfaces - ICMI

  15. IGFBP-3, hypoxia and TNF-{alpha} inhibit adiponectin transcription

    SciTech Connect (OSTI)

    Zappala, Giovanna, E-mail: zappalag@mail.nih.gov [Diabetes Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD (United States)] [Diabetes Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD (United States); Rechler, Matthew M. [Diabetes Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD (United States) [Diabetes Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD (United States); Clinical Endocrinology Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD (United States)

    2009-05-15T23:59:59.000Z

    The thiazolidinedione rosiglitazone, an agonist ligand for the nuclear receptor PPAR-{gamma}, improves insulin sensitivity in part by stimulating transcription of the insulin-sensitizing adipokine adiponectin. It activates PPAR-{gamma}-RXR-{alpha} heterodimers bound to PPAR-{gamma} response elements in the adiponectin promoter. Rosiglitazone-stimulated adiponectin protein synthesis in 3T3-L1 mouse adipocytes has been shown to be inhibited by IGFBP-3, which can be induced by hypoxia and the proinflammatory cytokine, TNF-{alpha}, two inhibitors of adiponectin transcription. The present study demonstrates that IGFBP-3, the hypoxia-mimetic agent cobalt chloride, and TNF-{alpha} inhibit rosiglitazone-induced adiponectin transcription in mouse embryo fibroblasts that stably express PPAR-{gamma}2. Native IGFBP-3 can bind RXR-{alpha} and inhibited rosiglitazone stimulated promoter activity, whereas an IGFBP-3 mutant that does not bind RXR-{alpha} did not. These results suggest that IGFBP-3 may mediate the inhibition of adiponectin transcription by hypoxia and TNF-{alpha}, and that IGFBP-3 binding to RXR-{alpha} may be required for the observed inhibition.

  16. activates nuclear transcription: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    activates nuclear transcription First Page Previous Page 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 Next Page Last Page Topic Index 1 Regulation of Nuclear...

  17. activated nuclear transcription: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    activated nuclear transcription First Page Previous Page 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 Next Page Last Page Topic Index 1 Regulation of Nuclear...

  18. RESEARCH ARTICLE Open Access A fungal endophyte induces transcription of

    E-Print Network [OSTI]

    Raizada, Manish N.

    RESEARCH ARTICLE Open Access A fungal endophyte induces transcription of genes encoding a redundant trees, proposed ecologically to act as a fungicide. Taxus is host to fungal endophytes, defined as organisms that inhabit plants without causing disease. The Taxus endophytes have been shown to synthesize

  19. Computational Comparative Study of Transcription Regulation in Eukaryotes

    E-Print Network [OSTI]

    Friedman, Nir

    interacting mechanisms. These mechanisms include both sequence specific binding proteins called transcription at promoter and enhancer regions, which affect the binding of regulatory proteins. While trans acting factors by the lack of large- scale and systematic experimental studies, and by the noisy nature of computational

  20. Multiple roles for the zebrafish transcriptional activator SBF/Staf

    E-Print Network [OSTI]

    Halbig, Kari Michele

    2009-05-15T23:59:59.000Z

    protein that enables it to activate the transcription of genes encoding tyrosinase and other proteins of the melanin- formation pathway (71). The FGFR1 gene contains an SPH element in the promoter of the human gene (60) and also has an SPH element...

  1. Transcriptional responses of insulin resistance and mechanisms of drug action

    E-Print Network [OSTI]

    Hsiao, Gene

    2010-01-01T23:59:59.000Z

    Pyruvate Srebf1 Srebf2 Pdk4 PDC Srebf1 Acat Malonyl-CoA FCTZD Post-TZD Pyruvate Akt Transcription Pyruvate PDK4 PDC-(P) PDC ACAS2L Acetate NR d IR d NR d IR d NR d IR d NR d IR

  2. Finding scientific articles 1. Find a specific article using google scholar or

    E-Print Network [OSTI]

    Schweik, Charles M.

    are connected through UMass ­ most articles are only available through academic libraries #12;Finding scientific#12;Finding scientific articles 1. Find a specific article using google scholar or web of science by searching for author name + title, year or journal name Note: you can only download articles if you

  3. SUPPLEMENTARY METHODS M1: ALGORITHM TO RECONSTRUCT TRANSCRIPTIONAL NETWORKS ..................................................M-2

    E-Print Network [OSTI]

    Babu, M. Madan

    M-1 SUPPLEMENTARY METHODS M1: ALGORITHM TO RECONSTRUCT TRANSCRIPTIONAL NETWORKS ..................................................M-2 Figure 1: Procedure to reconstruct transcriptional regulatory networks ....................................M-2 M2: PROCEDURE TO IDENTIFY ORTHOLOGOUS PROTEINS...................................................................M

  4. Polymorphisms in signal transducer and activator of transcription 3 and lung function in asthma

    E-Print Network [OSTI]

    Litonjua, Augusto A

    Background: Identifying genetic determinants for lung function is important in providing insight into the pathophysiology of asthma. Signal transducer and activator of transcription 3 is a transcription factor latent in ...

  5. Functional genomics analysis of the arabidopsis ABI5 bZIP transcription factor

    E-Print Network [OSTI]

    Hur, Jung-Im

    2009-05-15T23:59:59.000Z

    zipper (bZIP) transcription factors are identified by interaction with ABA responsive cis-regulatory elements. The transcription factor ABI5 is one of these. It regulates gene expression during embryogenesis and in response to ABA. An ABA...

  6. Genomes & Developmental Control Isl1 is a direct transcriptional target of Forkhead transcription factors in second heart

    E-Print Network [OSTI]

    factors in second heart field-derived mesoderm Jione Kang a , Elisha Nathan b , Shan-Mei Xu a , Eldad 2009 Available online 4 July 2009 Keywords: Isl1 Second heart field Anterior heart field Transgenic Transcription factor Forkhead FoxC2 FoxA2 FoxF1 Mouse The cells of the second heart field (SHF) contribute

  7. Transcriptional leakage versus noise: A simple mechanism of conversion between binary and graded response in autoregulated genes

    E-Print Network [OSTI]

    Anna Ochab-Marcinek; Marcin Tabaka

    2015-01-16T23:59:59.000Z

    We study the response of an autoregulated gene to a range of concentrations of signal molecules. We show that transcriptional leakage and noise due to translational bursting have the opposite effects. In a positively autoregulated gene, increasing the noise converts the response from graded to binary, while increasing the leakage converts the response from binary to graded. Our findings support the hypothesis that, being a common phenomenon, leaky expression may be a relatively easy way for evolutionary tuning of the type of gene response without changing the type of regulation from positive to negative.

  8. Sustainable Energy Resources for Consumers Webinar on Residential Geothermal Heat Pump Retrofit Transcript

    Broader source: Energy.gov [DOE]

    Transcript for a U.S. Department of Energy Webinar on Dec. 14, 2010, about residential geothermal heat pump retrofits

  9. Find us on Facebook myscienceacademy.org

    E-Print Network [OSTI]

    Chiao, Jung-Chih

    Find us on Facebook myscienceacademy.org 144,123 people like myscienceacademy.org. Facebook social In Recommendations Log in to Facebook to see your friends' recommendations. 24 Places That Look Not Normal this. 10 Famous Drug Myths 559 people recommend this. Facebook social plugin Share by Email · reddit

  10. Brain Imaging Findings in Neuropathic Pain

    E-Print Network [OSTI]

    Apkarian, A. Vania

    Brain Imaging Findings in Neuropathic Pain Paul Y. Geha, MD and A. Vania Apkarian, PhD* Address (posther- petic neuralgia), then the spinal cord (postspinal cord injury pain), to the brain itself mechanisms; hence, they assume implicitly or explicitly that the rest of the brain passively responds

  11. Proof positive : finding the cause of AIDS

    E-Print Network [OSTI]

    Rulison, Megan R. (Megan Rebecca)

    2008-01-01T23:59:59.000Z

    In 2008, it will have been 25 years since HIV was first isolated from a patient with AIDS. In the early 1980s, when the mysterious disease of the immune system spread across the globe, scientists began a race to find the ...

  12. An interesting finding in sup 229 Th

    SciTech Connect (OSTI)

    Reich, C.W.

    1990-01-01T23:59:59.000Z

    Work at INEL has recently established that the first excited states of {sup 229}Th forms a closely spaced doublet with the ground state, the separation being 1 {plus minus} 4 electron volts. A discussion of the data and the reasoning supporting this unprecedented finding is given. Some potential applications are briefly mentioned. 27 refs., 3 figs., 2 tabs.

  13. REPORT OF FINDINGS: National Science Foundation

    E-Print Network [OSTI]

    .........................................................................................................4 Chapter 2: Description of the Learning Community Projects and Types of Products..........7 ChapterREPORT OF FINDINGS: National Science Foundation Early Childhood Materials Development Learning..................................................................................................................20 What are the challenges in adapting materials for learning differences? What strategies were used

  14. Finding slowly decaying observables Gary Froyland \\Lambda

    E-Print Network [OSTI]

    Froyland, Gary

    Finding slowly decaying observables Gary Froyland \\Lambda Department of Mathematical Engineering initial transient behaviour to disappear. We present a rigorous numerical method for (i) estimating distribution on M ; that is, if you plot the orbit on a computer, you see the same distribution of dots. We

  15. Finding Acceptable Solutions Faster Using Inadmissible Information

    E-Print Network [OSTI]

    Ruml, Wheeler

    explicit estimation search (EES), a bounded suboptimal search algorithm that uses unbiased cost(n)+h(n) becomes f (n) = g(n)+w·h(n). The weight, w, increases the importance of h (estimated cost of reaching attempt to find a solution quickly while guaranteeing that its cost does not exceed optimal by more than

  16. Transcriptional control in Alicyclobacillus acidocaldarius and associated genes, proteins, and methods

    DOE Patents [OSTI]

    Lee, Brady Deneys; Thompson, David N; Apel, William A.; Thompson, Vicki Slavchev; Reed, David W; Lacey, Jeffrey A

    2014-05-06T23:59:59.000Z

    Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods of modulating transcription or transcription or transcriptional control using isolated and/or purified polypeptides and nucleic acid sequences from Alicyclobacillus acidocaldarius.

  17. Transcriptional networks: reverse-engineering gene regulation on a global scale

    E-Print Network [OSTI]

    Babu, M. Madan

    understand the transcriptional control of each gene and the targets of each transcription factor. In yeast, cis regulatory elements, and transcription factor DNA binding sites in vivo. Methods for modeling). This provides a mechanism to control specific aspects of phy- siology; it also enables the use of gene co

  18. Genome-wide In-silico Identification of Transcriptional Regulators Controlling Cell Cycle in Human Cells

    E-Print Network [OSTI]

    Shamir, Ron

    - 1 - Genome-wide In-silico Identification of Transcriptional Regulators Controlling Cell Cycle-3-6409760 Fax: 972-3-6407471 e-mail: yossih@post.tau.ac.il Running title: Transcriptional regulation of human cell cycle Key words: Transcriptional regulation, cell cycle, functional genomics. #12;- 2 - Abstract

  19. APOLLO 17 VOICE TRANSCRIPT PERTAINING TO THE GEOLOGY OF THE LANDING SITE

    E-Print Network [OSTI]

    Rathbun, Julie A.

    APOLLO 17 VOICE TRANSCRIPT PERTAINING TO THE GEOLOGY OF THE LANDING SITE #12;APOLLO 17 VOICE TRANSCRIPT Pertaining to the geology of the landing site by N.G. Bai ley and G.E. Ulrich U.s. Geological 5. Report Date Apollo 17 Voice Transcript 1975 Pertaining to the Geology of the Landing Site 6. 7

  20. Connections between mRNA 3( end processing and transcription termination

    E-Print Network [OSTI]

    Connections between mRNA 3( end processing and transcription termination Stephen Buratowski with transcription elongation and termination. It has been known for quite a long time that transcription termination The connection between polyadenylation and transcrip- tion termination was established early on with the dis

  1. RESEARCH Open Access Cell type-specific termination of transcription by

    E-Print Network [OSTI]

    Jordan, King

    RESEARCH Open Access Cell type-specific termination of transcription by transposable element sequences necessary for their own transposition, including signals required for the termination to terminate the transcription of host gene transcripts. While there is evidence in support of this model

  2. Lipid metabolism enzyme 5-LOX and its metabolite LTB4 are capable of activating transcription factor NF-{kappa}B in hepatoma cells

    SciTech Connect (OSTI)

    Zhao, Yu [Department of Biochemistry, College of Life Sciences, State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300071 (China)] [Department of Biochemistry, College of Life Sciences, State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300071 (China); Wang, Wenhui [Department of Interventional Radiography, First Hospital of Lanzhou University, No.1 Donggang West Road, Lanzhou, Gansu Province 730000 (China)] [Department of Interventional Radiography, First Hospital of Lanzhou University, No.1 Donggang West Road, Lanzhou, Gansu Province 730000 (China); Wang, Qi [Department of Cancer Research, Institute for Molecular Biology, College of Life Sciences, Nankai University, Tianjin 300071 (China)] [Department of Cancer Research, Institute for Molecular Biology, College of Life Sciences, Nankai University, Tianjin 300071 (China); Zhang, Xiaodong, E-mail: zhangxd@nankai.edu.cn [Department of Cancer Research, Institute for Molecular Biology, College of Life Sciences, Nankai University, Tianjin 300071 (China)] [Department of Cancer Research, Institute for Molecular Biology, College of Life Sciences, Nankai University, Tianjin 300071 (China); Ye, Lihong, E-mail: yelihong@nankai.edu.cn [Department of Biochemistry, College of Life Sciences, State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300071 (China)] [Department of Biochemistry, College of Life Sciences, State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300071 (China)

    2012-02-24T23:59:59.000Z

    Highlights: Black-Right-Pointing-Pointer 5-LOX is able to upregulate expression of NF-{kappa}B p65. Black-Right-Pointing-Pointer 5-LOX enhances nuclear translocation of NF-{kappa}B p65 via increasing p-I{kappa}B-{alpha} level. Black-Right-Pointing-Pointer 5-LOX stimulates transcriptional activity of NF-{kappa}B in hepatoma cells. Black-Right-Pointing-Pointer LTB4 activates transcriptional activity of NF-{kappa}B in hepatoma cells. -- Abstract: The issue that lipid metabolism enzyme and its metabolites regulate transcription factors in cancer cell is not fully understood. In this study, we first report that the lipid metabolism enzyme 5-Lipoxygenase (5-LOX) and its metabolite leukotriene B4 (LTB4) are capable of activating nuclear factor-{kappa}B (NF-{kappa}B) in hepatoma cells. We found that the treatment of MK886 (an inhibitor of 5-LOX) or knockdown of 5-LOX was able to downregulate the expression of NF-{kappa}B p65 at the mRNA level and decreased the phosphorylation level of inhibitor {kappa}B{alpha} (I{kappa}B{alpha}) in the cytoplasm of hepatoma HepG2 or H7402 cells, which resulted in the decrease of the level of nuclear NF-{kappa}B p65. These were confirmed by immunofluorescence staining in HepG2 cell. Moreover, the above treatments were able to decrease the transcriptional activity of NF-{kappa}B in the cells. The LTB4, one of metabolites of 5-LOX, is responsible for 5-LOX-activated NF-{kappa}B in a dose-dependent manner. Thus, we conclude that the lipid metabolism enzyme 5-LOX and its metabolite LTB4 are capable of activating transcription factor NF-{kappa}B in hepatoma cells. Our finding provides new insight into the significance of lipid metabolism in activation of transcription factors in cancer.

  3. Physical properties of naked DNA influence nucleosome positioning and correlate with transcription start and termination sites in yeast

    E-Print Network [OSTI]

    Deniz, Özgen; Flores, Oscar; Battistini, Federica; Pérez, Alberto; Soler-López, Montserrat; Orozco, Modesto

    2011-01-01T23:59:59.000Z

    with transcription start and termination sites in yeast. BMCtranscription start and termination sites in yeast Özgenand TTS (Transcription Termination Site) (at least in yeast)

  4. Transcript Request Form Note that any current or former student with a UTLN may request a transcript through iSIS at

    E-Print Network [OSTI]

    Dennett, Daniel

    a transcript through iSIS at http://go.tufts.edu/isis Please complete this form to request a Sackler School

  5. Efficiency and versatility of distal multisite transcription regulation

    E-Print Network [OSTI]

    Leonor Saiz; Jose M. G. Vilar

    2007-04-24T23:59:59.000Z

    Transcription regulation typically involves the binding of proteins over long distances on multiple DNA sites that are brought close to each other by the formation of DNA loops. The inherent complexity of the assembly of regulatory complexes on looped DNA challenges the understanding of even the simplest genetic systems, including the prototypical lac operon. Here we implement a scalable quantitative computational approach to analyze systems regulated through multiple DNA sites with looping. Our approach applied to the lac operon accurately predicts the transcription rate over five orders of magnitude for wild type and seven mutants accounting for all the combinations of deletions of the three operators. A quantitative analysis of the model reveals that the presence of three operators provides a mechanism to combine robust repression with sensitive induction, two seemingly mutually exclusive properties that are required for optimal functioning of metabolic switches.

  6. Finite Model Finding for Parameterized Verification

    E-Print Network [OSTI]

    Lisitsa, Alexei

    2010-01-01T23:59:59.000Z

    In this paper we investigate to which extent a very simple and natural "reachability as deducibility" approach, originated in the research in formal methods in security, is applicable to the automated verification of large classes of infinite state and parameterized systems. The approach is based on modeling the reachability between (parameterized) states as deducibility between suitable encodings of states by formulas of first-order predicate logic. The verification of a safety property is reduced to a pure logical problem of finding a countermodel for a first-order formula. The later task is delegated then to the generic automated finite model building procedures. In this paper we first establish the relative completeness of the finite countermodel finding method (FCM) for a class of parameterized linear arrays of finite automata. The method is shown to be at least as powerful as known methods based on monotonic abstraction and symbolic backward reachability. Further, we extend the relative completeness of ...

  7. Interferometric direction finding with a metamaterial detector

    SciTech Connect (OSTI)

    Venkatesh, Suresh; Schurig, David, E-mail: david.schurig@utah.edu [Department of Electrical and Computer Engineering, University of Utah, Salt Lake City, Utah 84112 (United States)] [Department of Electrical and Computer Engineering, University of Utah, Salt Lake City, Utah 84112 (United States); Shrekenhamer, David; Padilla, Willie [Department of Physics, Boston College, Chestnut Hill, Massachusetts 02467 (United States)] [Department of Physics, Boston College, Chestnut Hill, Massachusetts 02467 (United States); Xu, Wangren; Sonkusale, Sameer [Department of Electrical and Computer Engineering, Tufts University, Medford, Massachusetts 02155 (United States)] [Department of Electrical and Computer Engineering, Tufts University, Medford, Massachusetts 02155 (United States)

    2013-12-16T23:59:59.000Z

    We present measurements and analysis demonstrating useful direction finding of sources in the S band (2–4?GHz) using a metamaterial detector. An augmented metamaterial absorber that supports magnitude and phase measurement of the incident electric field, within each unit cell, is described. The metamaterial is implemented in a commercial printed circuit board process with off-board back-end electronics. We also discuss on-board back-end implementation strategies. Direction finding performance is analyzed for the fabricated metamaterial detector using simulated data and the standard algorithm, MUtiple SIgnal Classification. The performance of this complete system is characterized by its angular resolution as a function of radiation density at the detector. Sources with power outputs typical of mobile communication devices can be resolved at kilometer distances with sub-degree resolution and high frame rates.

  8. Commercial Building Energy Asset Score: Pilot Findings and Program...

    Office of Environmental Management (EM)

    Score: Pilot Findings and Program Update Commercial Building Energy Asset Score: Pilot Findings and Program Update The webinar was held on April 16, 2014, to share the findings...

  9. EA-1336: Finding of No Significant Impact | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    336: Finding of No Significant Impact EA-1336: Finding of No Significant Impact Ocean Sequestration of Carbon Dioxide Field Experiment This Finding of No Significant Impact is made...

  10. http://www.patent.gov.uk/patent/p-find/p-find-number Patents status information

    E-Print Network [OSTI]

    Colquhoun, David

    http://www.patent.gov.uk/patent/p-find/p-find-number Patents status information FULL DETAILS2409644 dated 06.07.2005 Examination requested 31.12.2004 Grant of Patent (Notification under Section 18(4)) 22.11.2005 Publication of notice in the Patents and Designs Journal (Section 25(1)) 21.12.2005 Title

  11. Finding Mentors and Creating aFinding Mentors and Creating a Supportive Climate

    E-Print Network [OSTI]

    Tilbury, Dawn

    Finding Mentors and Creating aFinding Mentors and Creating a Supportive Climate The academic mentoring relationships in addition to mentoring in more contemporary terms, where mentoring occurs as well as the classic mentors. We will also explore strategies for creating your own peer mentoring

  12. HOW TO FIND A JOB Candidates looking for jobs and employers seeking candidates find each

    E-Print Network [OSTI]

    Suzuki, Masatsugu

    HOW TO FIND A JOB Candidates looking for jobs and employers seeking candidates find each other in a variety of ways. In any economy, a job search should utilize many strategies including resources a short-term position. The question to ask yourself before you begin your search is "What kind of job

  13. Best Possible Strategy for Finding Ground States

    SciTech Connect (OSTI)

    Franz, Astrid; Hoffmann, Karl Heinz; Salamon, Peter

    2001-06-04T23:59:59.000Z

    Finding the ground state of a system with a complex energy landscape is important for many physical problems including protein folding, spin glasses, chemical clusters, and neural networks. Such problems are usually solved by heuristic search methods whose efficacy is judged by empirical performance on selected examples. We present a proof that, within the large class of algorithms that simulate a random walk on the landscape, threshold accepting is the best possible strategy. In particular, it can perform better than simulated annealing and Tsallis statistics. Our proof is the first example of a provably optimal strategy in this area.

  14. Find R&D Results

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of Science (SC) Environmental Assessments (EA)Budget »Travel andFifthDepartmentPatents Find380,000

  15. Engineering phenolics metabolism in the grasses using transcription factors

    SciTech Connect (OSTI)

    Grotewold, Erich [The Ohio State University

    2013-07-26T23:59:59.000Z

    The economical competitiveness of agriculture-derived biofuels can be significantly enhanced by increasing biomass/acre yields and by furnishing the desired carbon balance for facilitating liquid fuel production (e.g., ethanol) or for high-energy solid waste availability to be used as biopower (e.g., for electricity production). Biomass production and carbon balance are tightly linked to the biosynthesis of phenolic compounds, which are found in crops and in agricultural residues either as lignins, as part of the cell wall, or as soluble phenolics which play a variety of functions in the biology of plants. The grasses, in particular maize, provide the single major source of agricultural biomass, offering significant opportunities for increasing renewable fuel production. Our laboratory has pioneered the use of transcription factors for manipulating plant metabolic pathways, an approach that will be applied here towards altering the composition of phenolic compounds in maize. Previously, we identified a small group of ten maize R2R3-MYB transcription factors with all the characteristics of regulators of different aspects of phenolic biosynthesis. Here, we propose to investigate the participation of these R2R3-MYB factors in the regulation of soluble and insoluble maize phenolics, using a combination of over-expression and down-regulation of these transcription factors in transgenic maize cultured cells and in maize plants. Maize cells and plants altered in the activity of these regulatory proteins will be analyzed for phenolic composition by targeted metabolic profiling. Specifically, we will I) Investigate the effect of gain- and loss-of-function of a select group of R2R3-MYB transcription factors on the phenolic composition of maize plants and II) Identify the biosynthetic genes regulated by each of the selected R2R3-MYB factors. While a likely outcome of these studies are transgenic maize plants with altered phenolic composition, this research will significantly contribute to understanding how different branches of the phenolic biosynthetic grid are regulated. Given the conservation of the selected regulators in other grasses, results derived from this project are likely to provide important tools for the manipulation of phenolic compounds in other emerging biomass producers (e.g., switchgrass or miscanthus), either through conventional breeding techniques (e.g., marker-assisted breeding) or by using transgenic approaches.

  16. HIV transcription is induced with some forms of cell killing

    SciTech Connect (OSTI)

    Woloschak, G.E. [Argonne National Lab., IL (United States); Schreck, S. [Argonne National Lab., IL (United States)][South Carolina Univ., Columbia, SC (United States). Dept. of Chemistry; Panozzo, J. [Loyola Univ. Medical Center, Maywood, IL (United States); Chang-Liu, C.-M. [Argonne National Lab., IL (United States); Libertin, C.R. [Loyola Univ. Medical Center, Maywood, IL (United States)

    1996-11-01T23:59:59.000Z

    Using HeLa cells stably transfected with an HIV-LTR-CAT construct`, we demonstrated a peak in CAT induction that occurs in viable (but not necessarily cell-division-competent) cells 24 h following exposure to some cell-killing agents. {Gamma} rays were the only cell-killing agent which did not induce HIV transcription; this can be attributed to the fact that {gamma}-ray-induced apoptotic death requires function p53, which is missing in HeLa cells. For all other agents, HIV-LTR induction was dose-dependent and correlated with the amount of cell killing that occurred in the culture.

  17. CSC Workshop Transcript 02-20-2105 | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742Energy China U.S. Department ofJune 2,TheEnergy December 14, 2007Workshop Transcript

  18. Determining physical constraints in transcriptional initiationcomplexes using DNA sequence analysis

    SciTech Connect (OSTI)

    Shultzaberger, Ryan K.; Chiang, Derek Y.; Moses, Alan M.; Eisen,Michael B.

    2007-07-01T23:59:59.000Z

    Eukaryotic gene expression is often under the control ofcooperatively acting transcription factors whose binding is limited bystructural constraints. By determining these structural constraints, wecan understand the "rules" that define functional cooperativity.Conversely, by understanding the rules of binding, we can inferstructural characteristics. We have developed an information theory basedmethod for approximating the physical limitations of cooperativeinteractions by comparing sequence analysis to microarray expressiondata. When applied to the coordinated binding of the sulfur amino acidregulatory protein Met4 by Cbf1 and Met31, we were able to create acombinatorial model that can correctly identify Met4 regulatedgenes.

  19. Transcript from 12/01/08 | Department of Energy

    Energy Savers [EERE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directedAnnual Siteof Energy 2, 2015 - JanuaryTankToledo, Ohio, DataTechnical Assistance »Transcript

  20. Transcript: OSTI Highlights First-Time Electronic Availability of

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of ScienceandMesa del SolStrengthening a solidSynthesisAppliances » TopToursTrainingTranscript

  1. Electricity Advisory Committee Meeting, May 20, 2008 (TRANSCRIPT) |

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742Energy Chinaof EnergyImpactOn July 2, 2014 in theGroupJune 6, 2013and Transcripts

  2. Finding Nonoverlapping Substructures of a Sparse Matrix

    SciTech Connect (OSTI)

    Pinar, Ali; Vassilevska, Virginia

    2005-08-11T23:59:59.000Z

    Many applications of scientific computing rely on computations on sparse matrices. The design of efficient implementations of sparse matrix kernels is crucial for the overall efficiency of these applications. Due to the high compute-to-memory ratio and irregular memory access patterns, the performance of sparse matrix kernels is often far away from the peak performance on a modern processor. Alternative data structures have been proposed, which split the original matrix A into A{sub d} and A{sub s}, so that A{sub d} contains all dense blocks of a specified size in the matrix, and A{sub s} contains the remaining entries. This enables the use of dense matrix kernels on the entries of A{sub d} producing better memory performance. In this work, we study the problem of finding a maximum number of nonoverlapping dense blocks in a sparse matrix, which is previously not studied in the sparse matrix community. We show that the maximum nonoverlapping dense blocks problem is NP-complete by using a reduction from the maximum independent set problem on cubic planar graphs. We also propose a 2/3-approximation algorithm that runs in linear time in the number of nonzeros in the matrix. This extended abstract focuses on our results for 2x2 dense blocks. However we show that our results can be generalized to arbitrary sized dense blocks, and many other oriented substructures, which can be exploited to improve the memory performance of sparse matrix operations.

  3. afg3l2 transcripts encoding: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    into regulatory rich different transcription factors. We perform on all the factors a multivariate analysis in the framework Gerstein, Mark 9 High Nucleosome Occupancy Is...

  4. Resources for Handling Transcripts | U.S. DOE Office of Science...

    Office of Science (SC) Website

    Print Text Size: A A A FeedbackShare Page Uploading an Acceptable Transcript Scanning a Paper Document Removing PII from a Paper Document Converting an Electronic Document...

  5. Turning behavior in Drosophila larvae: a role for the small scribbler transcript

    E-Print Network [OSTI]

    Sokolowski, Marla

    - tify molecules regulating motor development in model organ- isms amenable to genetic manipulation are not well understood. Genes controlling the development of locomotor networks likely encode transcription

  6. Transcript of April 3, 2013, TAP webinar titled State Energy Strategic Planning

    Broader source: Energy.gov [DOE]

    U.S. Department of Energy (DOE) Technical Assistance Program (TAP) transcript of a TAP webinar held on April 3, 2013 and dealing with state energy strategic planning.

  7. Advancement and analysis of Gauss pseudospectral transcription for optimal control problems

    E-Print Network [OSTI]

    Huntington, Geoffrey Todd, 1979-

    2007-01-01T23:59:59.000Z

    As optimal control problems become increasingly complex, innovative numerical methods are needed to solve them. Direct transcription methods, and in particular, methods involving orthogonal collocation have become quite ...

  8. EA-1839: Final Environmental Assessment and Finding of No Significant...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    9: Final Environmental Assessment and Finding of No Significant Impact EA-1839: Final Environmental Assessment and Finding of No Significant Impact Department of Energy Loan...

  9. EA-1665: Final Environmental Assessment and Finding of No Significant...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Final Environmental Assessment and Finding of No Significant Impact EA-1665: Final Environmental Assessment and Finding of No Significant Impact Davis-Kingman Tap 69-kV...

  10. EA-1178: Finding of No Significant Impact | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    Finding of No Significant Impact EA-1178: Finding of No Significant Impact 300 Area Steam Plant Replacement, Hanford Site, Richland, Washington Based on the analysis in the EA,...

  11. Finance Program Retention for FALL 2014 Instructions for Transcript Review Form

    E-Print Network [OSTI]

    Kostic, Milivoje M.

    Finance Program ­ Retention for FALL 2014 Instructions for Transcript Review Form College of Business at Northern Illinois University The Department of Finance Transcript Review Form should in the finance core). All NIU students are eligible to declare a major in Finance; however, students must meet

  12. Transcriptional regulatory networks downstream of TAL1/SCL in T-cell acute lymphoblastic leukemia

    E-Print Network [OSTI]

    Liu, Xiaole Shirley

    pathogene- sis of human T-cell acute lymphoblastic leukemia (T-ALL); however, oncogenic transcriptional expression leads to T-cell acute lymphoblastic leukemia (T-ALL). TAL1 is expressed by the leukemic cellsNEOPLASIA Transcriptional regulatory networks downstream of TAL1/SCL in T-cell acute lymphoblastic

  13. DNA binding shifts the redox potential of the transcription factor SoxR

    E-Print Network [OSTI]

    Dietrich, Lars

    DNA binding shifts the redox potential of the transcription factor SoxR Alon A. Gorodetsky , Lars E-modified electrodes are used to probe the effects of binding to DNA on the redox potential of SoxR, a transcription in the absence of DNA. Using Redmond red as a covalently bound redox reporter affixed above the SoxR binding site

  14. APOLLO 11 .V O ICE TRANSCRIPT PERTAINING TO THE GEOLOGY OF THE LANDING SITE

    E-Print Network [OSTI]

    Rathbun, Julie A.

    * * *: {( APOLLO 11 .V O ICE TRANSCRIPT PERTAINING TO THE GEOLOGY OF THE LANDING SITE #12;APOLLO 11 VOICE TRANSCRIPT Pertaining to the geology of the landing site by N. G. Bailey and G. E. Ulrich U.S. Geological Survey Branch of Astrogeology Flagstaff, Arizona 1974 #12;USCOMM·OC 8265-P74THIS ~ORM 1\\1A) HI

  15. APOLLO 16 VOICE TRANSCRIPT PERTAINING TO THE GEOLOGY OF THE LANDING SITE

    E-Print Network [OSTI]

    Rathbun, Julie A.

    * * *: {( APOLLO 16 VOICE TRANSCRIPT PERTAINING TO THE GEOLOGY OF THE LANDING SITE #12;- APOLLO 16 VOICE TRANSCRIPT Pertaining to the geology of the landIng site by N.G. Bai loey and G.E. Ulrich U.s. Geol:ogical Survey Branch of Astrogeology F]agstaff~ Arizona 1915 #12;FORM NTlS·315 UO-70

  16. APOLLO 15 VOICE TRANSCRIPT PERTAINING TO THE GEOLOGY OF THE LANDING SITE

    E-Print Network [OSTI]

    Rathbun, Julie A.

    * * *: {( APOLLO 15 VOICE TRANSCRIPT PERTAINING TO THE GEOLOGY OF THE LANDING SITE #12;APOLLO 15 VOICE TRANSCRIPT Pertaining to the geology of the landing site ~ N.G. Bailey and G.E. Ulrich U.S. Geological Survey Branch of Astrogeology Flagstaff, Arizona 1~5 #12;BIBLIOGRAPHICDATA II. Report No. J2. 3

  17. * * * *APOLLO 12 VOICE TRANSCRIPT PERTAINING TO THE GEOLOGY OF THE LANDING SITE

    E-Print Network [OSTI]

    Rathbun, Julie A.

    * * * *APOLLO 12 VOICE TRANSCRIPT PERTAINING TO THE GEOLOGY OF THE LANDING SITE #12;APOLLO 12 VOICE TRANSCRIPT Pertaining to the geology of the landing site by N.G. Bailey and G.E. Ulrich U.S. Geological to the Geology of the Landing Site 7. Auchor(s) 8. Performing Organization Repr. N. G. Bailey and G. E. Ulrich No

  18. APOLLO 14 VOICE TRANSCRIPT PERTAINING TO THE GEOLOGY OF THE LANDING SITE

    E-Print Network [OSTI]

    Rathbun, Julie A.

    * * *: {( APOLLO 14 VOICE TRANSCRIPT PERTAINING TO THE GEOLOGY OF THE LANDING SITE #12;APOLLO 14 VOICE TRANSCRIPT Pertaining to the geology of the landing site by N.G. Bailey and G.E. UI rich U.S. Geological Survey Branch of Astrogeology Flagstaff, Arizona 1975 #12;nils fOR.1 ~t.\\) IH. fl.HJ!Hl!ll( ".0

  19. The obesity-associated Fto gene is a transcriptional coactivator Qiong Wu a,1

    E-Print Network [OSTI]

    Toledo, University of

    The obesity-associated Fto gene is a transcriptional coactivator Qiong Wu a,1 , Rudel A. Saunders a September 2010 Available online 19 September 2010 Keywords: Obesity Adipogenesis Transcriptional coactivator Fto CCAAT/enhancer binding protein DNA methylation a b s t r a c t The fat mass and obesity associated

  20. Uncovering a Hidden Distributed Architecture Behind Scale-free Transcriptional Regulatory Networks

    E-Print Network [OSTI]

    Babu, M. Madan

    Uncovering a Hidden Distributed Architecture Behind Scale-free Transcriptional Regulatory Networks: transcriptional network; co-regulation network; distributed robustness; scale-free structure; evolution. This mutational robustness could arise either due to redundancy, i.e. direct backup, or due to distributed

  1. BAYESIAN INFERENCE FOR MODELS OF TRANSCRIPTIONAL REGULATION USING MARKOV CHAIN MONTE CARLO SAMPLING

    E-Print Network [OSTI]

    Opper, Manfred

    ]. In this contribution we present a Markov chain Monte Carlo (MCMC) sampler which infers the TF activity based on a modelBAYESIAN INFERENCE FOR MODELS OF TRANSCRIPTIONAL REGULATION USING MARKOV CHAIN MONTE CARLO SAMPLING]. Transcription of genes is controlled by proteins which can bind to particular base-sequences of DNA

  2. Genome-scale definition of the transcriptional programme associated with compromised PU.1 activity in Acute Myeloid Leukaemia

    E-Print Network [OSTI]

    Sive, Jonathan I.; Basilico, Silvia; Hannah, Rebecca; Kinston, Sarah J.; Calero-Nieto, Fernando J.; Göttgens, Berthold

    2015-01-01T23:59:59.000Z

    Transcriptional dysregulation is associated with haematological malignancy. Although mutations of the key haematopoietic transcription factor PU.1 are rare in human acute myeloid leukemia (AML), they are common in murine models of radiation...

  3. Genome-wide analysis of transcriptional expression programs, regulatory networks and Cis-regulatory sequences in Saccharomyces cerevisiae

    E-Print Network [OSTI]

    Harbison, Christopher T., 1973-

    2005-01-01T23:59:59.000Z

    Historically, knowledge of gene-specific transcription has been accumulated by the study of the individual genetic and physical interactions between transcriptional regulators and the genes they regulate, often requiring ...

  4. EA-1465: Finding of No Significant Impact | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    65: Finding of No Significant Impact EA-1465: Finding of No Significant Impact Wind Energy Center EdgeleyKulm Project, North Dakota Basin Electric Power Cooperative, on behalf of...

  5. FINDING THE MIND IN THE BODY Joshua Knobe

    E-Print Network [OSTI]

    Knobe, Joshua

    FINDING THE MIND IN THE BODY Joshua Knobe [Knobe, J. (2011). Finding the mind in the body. In Brockman, M. (ed.) Future Science: Essays from that seems especially noticeable. Some of these things have minds, while others do

  6. Got a Minute? Which Higgs did we find?

    ScienceCinema (OSTI)

    Stupak, John

    2014-09-15T23:59:59.000Z

    Dr. John Stupak talks about the discovery of the Higgs boson. Did scientists find the Higgs boson predicted back in 1964 or did they find just one of a group of particles, with the others still to be found?

  7. Got a Minute? Which Higgs did we find?

    SciTech Connect (OSTI)

    Stupak, John

    2014-06-26T23:59:59.000Z

    Dr. John Stupak talks about the discovery of the Higgs boson. Did scientists find the Higgs boson predicted back in 1964 or did they find just one of a group of particles, with the others still to be found?

  8. EA-1501: Finding of No Significant Impact | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    01: Finding of No Significant Impact EA-1501: Finding of No Significant Impact Construction, Operation, and Closure of the Burma Road II Borrow Pit at the Savannah River Site The...

  9. Former Construction Worker Finds New Career in Solar | Department...

    Broader source: Energy.gov (indexed) [DOE]

    Former Construction Worker Finds New Career in Solar Former Construction Worker Finds New Career in Solar August 25, 2010 - 4:00pm Addthis Eco Technologies, Inc., hired eleven...

  10. Extracellular Matrix-Regulated Gene Expression RequiresCooperation of SWI/SNF and Transcription Factors

    SciTech Connect (OSTI)

    Xu, Ren; Spencer, Virginia A.; Bissell, Mina J.

    2006-05-25T23:59:59.000Z

    Extracellular cues play crucial roles in the transcriptional regulation of tissue-specific genes, but whether and how these signals lead to chromatin remodeling is not understood and subject to debate. Using chromatin immunoprecipitation (ChIP) assays and mammary-specific genes as models, we show here that extracellular matrix (ECM) molecules and prolactin cooperate to induce histone acetylation and binding of transcription factors and the SWI/SNF complex to the {beta}- and ?-casein promoters. Introduction of a dominant negative Brg1, an ATPase subunit of SWI/SNF complex, significantly reduced both {beta}- and ?-casein expression, suggesting that SWI/SNF-dependent chromatin remodeling is required for transcription of mammary-specific genes. ChIP analyses demonstrated that the ATPase activity of SWI/SNF is necessary for recruitment of RNA transcriptional machinery, but not for binding of transcription factors or for histone acetylation. Coimmunoprecipitation analyses showed that the SWI/SNF complex is associated with STAT5, C/EBP{beta}, and glucocorticoid receptor (GR). Thus, ECM- and prolactin-regulated transcription of the mammary-specific casein genes requires the concerted action of chromatin remodeling enzymes and transcription factors.

  11. A Network of Relaxation Oscillators that Finds Downbeats in Rhythms

    E-Print Network [OSTI]

    Eck, Doug

    & Kolen [8] and McAuley [12] provide oscillator models that work online to find downbeats in temporal

  12. Transcriptional regulation of the Arabidopsis thaliana chalcone synthase gene

    SciTech Connect (OSTI)

    Feinbaum, R.L.; Ausubel, F.M.

    1988-05-01T23:59:59.000Z

    The authors cloned an Arabiodpsis thaliana chalcone synthase (CHS) gene on the basis of cross-hybridization with a Petroselinum hortense CHS cDNA clone. The protein sequence deduced from the A. thaliana CHS DNA sequence is at least 85% homologous to the CHS sequences from P. hortense, Antirrhinum majus, and Petunia hybrida. Southern blot analysis indicated that CHS is a single-copy gene in A. thaliana. High-intensity light treatment of A. thaliana plants for 24 h caused a 50-fold increase in CHS enzyme activity and an accumulation of visibly detectable levels of anthocyanin pigments in the vegetative structures of these plants. A corresponding increase in the steady-state level of CHS mRNA was detected after high-intensity light treatment for the same period of time. The accumulation of CHS mRNA in response to high-intensity light was due, at least in part, to an increased rate of transcription of the CHS gene as demonstrated by nuclear runoff experiment.

  13. Thyroid hormone receptor inhibits hepatoma cell migration through transcriptional activation of Dickkopf 4

    SciTech Connect (OSTI)

    Chi, Hsiang-Cheng; Liao, Chen-Hsin [Department of Biochemistry, School of Medicine, Chang-Gung University, Taoyuan 333, Taiwan, ROC (China)] [Department of Biochemistry, School of Medicine, Chang-Gung University, Taoyuan 333, Taiwan, ROC (China); Huang, Ya-Hui [Medical Research Central, Chang Gung Memorial Hospital, Taoyuan 333, Taiwan, ROC (China)] [Medical Research Central, Chang Gung Memorial Hospital, Taoyuan 333, Taiwan, ROC (China); Wu, Sheng-Ming; Tsai, Chung-Ying; Liao, Chia-Jung; Tseng, Yi-Hsin; Lin, Yang-Hsiang; Chen, Cheng-Yi; Chung, I-Hsiao; Wu, Tzu-I [Department of Biochemistry, School of Medicine, Chang-Gung University, Taoyuan 333, Taiwan, ROC (China)] [Department of Biochemistry, School of Medicine, Chang-Gung University, Taoyuan 333, Taiwan, ROC (China); Chen, Wei-Jan [First Cardiovascular Division, Chang Gung Memorial Hospital, Taoyuan 333, Taiwan, ROC (China)] [First Cardiovascular Division, Chang Gung Memorial Hospital, Taoyuan 333, Taiwan, ROC (China); Lin, Kwang-Huei, E-mail: khlin@mail.cgu.edu.tw [Department of Biochemistry, School of Medicine, Chang-Gung University, Taoyuan 333, Taiwan, ROC (China)] [Department of Biochemistry, School of Medicine, Chang-Gung University, Taoyuan 333, Taiwan, ROC (China)

    2013-09-13T23:59:59.000Z

    Highlights: •T{sub 3} affects DKK4 mRNA and protein expression in HepG2-TR cells. •Regulation of DKK4 by T{sub 3} is at transcriptional level. •DKK4 overexpression suppresses hepatoma cell metastasis. -- Abstract: Triiodothyronine (T{sub 3}) is a potent form of thyroid hormone mediates several physiological processes including cellular growth, development, and differentiation via binding to the nuclear thyroid hormone receptor (TR). Recent studies have demonstrated critical roles of T{sub 3}/TR in tumor progression. Moreover, long-term hypothyroidism appears to be associated with the incidence of human hepatocellular carcinoma (HCC), independent of other major HCC risk factors. Dickkopf (DKK) 4, a secreted protein that antagonizes the canonical Wnt signaling pathway, is induced by T{sub 3} at both mRNA and protein levels in HCC cell lines. However, the mechanism underlying T{sub 3}-mediated regulation of DKK4 remains unknown. In the present study, the 5? promoter region of DKK4 was serially deleted, and the reporter assay performed to localize the T{sub 3} response element (TRE). Consequently, we identified an atypical direct repeat TRE between nucleotides ?1645 and ?1629 conferring T{sub 3} responsiveness to the DKK4 gene. This region was further validated using chromatin immunoprecipitation (ChIP) and electrophoretic mobility shift assay (EMSA). Stable DKK4 overexpression in SK-Hep-1 cells suppressed cell invasion and metastatic potential, both in vivo andin vitro, via reduction of matrix metalloproteinase-2 (MMP-2) expression. Our findings collectively suggest that DKK4 upregulated by T{sub 3}/TR antagonizes the Wnt signal pathway to suppress tumor cell progression, thus providing new insights into the molecular mechanism underlying thyroid hormone activity in HCC.

  14. Transcriptional regulation of seed-specific gene expression - from PvALF/ ABI3 to phaseolin

    E-Print Network [OSTI]

    Ng, Wang Kit

    2006-10-30T23:59:59.000Z

    the inactive state in transgenic Arabidopsis leaves was accomplished by ectopic expression of the transcription factor PvALF (Phaseolus vulgaris ABI3-like factor), and application of abscisic acid (ABA). PvALF belongs to a family of seed...

  15. Regulation of cell fate by phosphorylation : a tale of two transcription factors

    E-Print Network [OSTI]

    Tootle, Tina Louise

    2004-01-01T23:59:59.000Z

    As transcription factors are the nuclear effectors of signaling cascades, mechanisms must exist to specifically regulate their activities in response to signaling events. Regulation can occur at the DNA, RNA, or protein ...

  16. Threonine 149 Phosphorylation Enhances  ?FosB Transcriptional Activity to Control Psychomotor Responses to Cocaine

    E-Print Network [OSTI]

    Neve, Rachael L.

    Stable changes in neuronal gene expression have been studied as mediators of addicted states. Of particular interest is the transcription factor ?FosB, a truncated and stable FosB gene product whose expression in nucleus ...

  17. The interaction of promoter chromatin architecture with the cell cycle regulates transcription activation kinetics

    E-Print Network [OSTI]

    Zopf, Christopher J. (Christopher John)

    2013-01-01T23:59:59.000Z

    The relationship between regulatory trans-factors binding a gene's cis-regulatory sequence elements and the transcriptional output of that gene is fundamental to even the most complex network behaviors such as metabolism ...

  18. Distinct Cyclin D Genes Show Mitotic Accumulation or Constant Levels of Transcripts in Tobacco

    E-Print Network [OSTI]

    Murray, J.A.H.

    Distinct Cyclin D Genes Show Mitotic Accumulation or Constant Levels of Transcripts in Tobacco-type cyclins accumulate peri- odically during the S, G2, and early M phases (A types) or G2 and early M phases

  19. Transcript of April 11, 2013, TAP webinar titled Financing Energy Upgrades for K-12 School Districts

    Broader source: Energy.gov [DOE]

    U.S. Department of Energy (DOE) Technical Assistance Program (TAP) transcript of a TAP webinar held on April 11, 2013 and dealing with how to finance energy efficiency upgrades for K-12 school districts.

  20. Genomic neighbourhood and the regulation of gene expression Genomic neighbourhood and transcriptional regulation

    E-Print Network [OSTI]

    Babu, M. Madan

    Genomic neighbourhood and the regulation of gene expression Genomic neighbourhood and transcriptional regulation Subhajyoti De and M. Madan Babu MRC Laboratory of Molecular Biology, Hills Road..................................................................................................................................................................................1 2. Genomic neighbourhood and its influence on gene regulation

  1. SciP : a novel inhibitor of CtrA transcriptional activity in Caulobacter crescentus

    E-Print Network [OSTI]

    Gora, Kasia G. (Kasia Gabriela)

    2011-01-01T23:59:59.000Z

    Cells must sense changes in their environment and respond appropriately in order to survive. A common survival strategy is for cells to translate an environmental signal into the activity of a transcription factor they ...

  2. Diverse patterns of genomic targeting by transcriptional regulators in Drosophila melanogaster

    E-Print Network [OSTI]

    Slattery, Matthew

    Annotation of regulatory elements and identification of the transcription-related factors (TRFs) targeting these elements are key steps in understanding how cells interpret their genetic blueprint and their environment ...

  3. Eric C. Achberger -molecular biology; transcription Christopher C. Austin -herpetology, systematics

    E-Print Network [OSTI]

    Stephens, Jacqueline

    - pathogenicity of Vibrio and Streptomyces William J. Platt III - plant populations and communities James VBIOLOGICAL SCIENCES Eric C. Achberger - molecular biology; transcription Christopher C. Austin Prosanta Chakrabarty - ichthyology; systematics Brent C. Christner - microbes in the cryosphere

  4. Experimental Incubations Elicit Profound Changes in Community Transcription in OMZ Bacterioplankton

    E-Print Network [OSTI]

    Stewart, Frank J.

    Sequencing of microbial community RNA (metatranscriptome) is a useful approach for assessing gene expression in microorganisms from the natural environment. This method has revealed transcriptional patterns in situ, but ...

  5. Analysis of variation at transcription factor binding sites in Drosophila and humans

    E-Print Network [OSTI]

    Spivakov, Mikhail

    Background: Advances in sequencing technology have boosted population genomics and made it possible to map the positions of transcription factor binding sites (TFBSs) with high precision. Here we investigate TFBS variability ...

  6. Large-Scale Mapping and Validation of Escherichia coli Transcriptional Regulation

    E-Print Network [OSTI]

    Collins, James J.

    Large-Scale Mapping and Validation of Escherichia coli Transcriptional Regulation from a Compendium Bioinformatics Program, Boston University, Boston, Massachusetts, United States of America, 2 Department of Biomedical Engineering, Boston University, Boston, Massachusetts, United States of America, 3 Boston

  7. 'I Saw You Disappear with My Own Eyes': Hidden Transcripts of New York Black Isrealite Bricolage

    E-Print Network [OSTI]

    Dorman, Jacob S.

    2007-08-01T23:59:59.000Z

    To date, scholars have tended to view Black Israelites as mercenary, derivative, or imitative. However, this microhistorical reading of the public, partial, and hidden transcripts of New York Rabbi Wentworth Arthur Matthew's ...

  8. Mechanisms of transcriptional activation of estrogen responsive genes in breast cancer cells 

    E-Print Network [OSTI]

    Chen, Chien-Cheng

    2009-06-02T23:59:59.000Z

    Estrogen receptor (ER) acts as a ligand-activated transcription factor that regulates the expression of genes. The genomic mechanisms of ER action include ligand-induced dimerization of ER which binds estrogen responsive elements (EREs...

  9. Mechanisms of transcriptional activation of estrogen responsive genes in breast cancer cells

    E-Print Network [OSTI]

    Chen, Chien-Cheng

    2009-06-02T23:59:59.000Z

    Estrogen receptor (ER) acts as a ligand-activated transcription factor that regulates the expression of genes. The genomic mechanisms of ER action include ligand-induced dimerization of ER which binds estrogen responsive elements (EREs...

  10. Swimming Upstream: Identifying Proteomic Signals that Drive Transcriptional Changes using the Interactome and Multiple “-Omics” Datasets

    E-Print Network [OSTI]

    Huang, Shao-shan Carol

    Signaling and transcription are tightly integrated processes that underlie many cellular responses to the environment. A network of signaling events, often mediated by post-translational modification on proteins, can lead ...

  11. Michigan State University (MSU) and Lake Michigan College (LMC) Reverse Transfer Agreement Transcript Release Form

    E-Print Network [OSTI]

    Michigan State University (MSU) and Lake Michigan College (LMC) Reverse Transfer Agreement Transcript Release Form Please complete, sign, and return this release form to: Michigan State University of the Registrar at Michigan State University in writing. SIGNATURE: _______________________________________ DATE

  12. Transcript of March 28, 2013, TAP webinar titled Internal Benchmarking Outreach and Data Collection Techniques

    Broader source: Energy.gov [DOE]

    U.S. Department of Energy (DOE) Technical Assistance Program (TAP) transcript of a TAP webinar held on March 28, 2013 and dealing with internal benchmarking outreach and data collection techniques.

  13. Chromatin-targeting small molecules cause class-specific transcriptional changes in pancreatic endocrine cells

    E-Print Network [OSTI]

    Kubicek, Stefan

    Under the instruction of cell-fate–determining, DNA-binding transcription factors, chromatin-modifying enzymes mediate and maintain cell states throughout development in multicellular organisms. Currently, small molecules ...

  14. Rapid Sequence Evolution of Transcription Factors Controlling Neuron Differentiation in Caenorhabditis

    E-Print Network [OSTI]

    Oregon, University of

    Rapid Sequence Evolution of Transcription Factors Controlling Neuron Differentiation phenotypic evolution proceeds predominantly through changes in regulatory sequences is a controversial issue in cis- regulatory sequences is an important determinant of phenotypic diversity. However, recent

  15. Helix Unwinding and Base Flipping Enable Human MTERF1 to Terminate Mitochondrial Transcription

    SciTech Connect (OSTI)

    Yakubovskaya, E.; Mejia, E; Byrnes, J; Hambardjieva, E; Garcia-Diaz, M

    2010-01-01T23:59:59.000Z

    Defects in mitochondrial gene expression are associated with aging and disease. Mterf proteins have been implicated in modulating transcription, replication and protein synthesis. We have solved the structure of a member of this family, the human mitochondrial transcriptional terminator MTERF1, bound to dsDNA containing the termination sequence. The structure indicates that upon sequence recognition MTERF1 unwinds the DNA molecule, promoting eversion of three nucleotides. Base flipping is critical for stable binding and transcriptional termination. Additional structural and biochemical results provide insight into the DNA binding mechanism and explain how MTERF1 recognizes its target sequence. Finally, we have demonstrated that the mitochondrial pathogenic G3249A and G3244A mutations interfere with key interactions for sequence recognition, eliminating termination. Our results provide insight into the role of mterf proteins and suggest a link between mitochondrial disease and the regulation of mitochondrial transcription.

  16. Transcriptional analysis of the healing response of wounded nerves treated with collagen and silicone tubes

    E-Print Network [OSTI]

    Wong, Matthew Q

    2008-01-01T23:59:59.000Z

    This study examines the transcriptional differences between nerve wounds treated with silicone tubes and those treated with collagen nerve regeneration templates. The primary motivation for the study is to test the hypothesis ...

  17. africa methodology findings: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    University of California eScholarship Repository Summary: for Development: Climate Change Policy for Africa. AfricanCLIMATE CHANGE MITIGATION SUMMARY PROJECTS: OF FINDINGS FROM...

  18. EA-1542: Finding of No Significant Impact | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    of No Significant Impact EA-1542: Finding of No Significant Impact Burleigh County Wind Energy Center, North Dakota Basin Electric Power Cooperative (Basin), on behalf of FPL...

  19. Finding of No Significant Impact Radiological/Nuclear Countermeasures...

    Broader source: Energy.gov (indexed) [DOE]

    FINDING OF NO SIGNIFICANT IMPACT RADIOLOGICALNUCLEAR COUNTERMEASURES TEST AND EVALUATION COMPLEX, NEVADA TEST SITE The U.S. Department of Homeland Security (DHS) is the Federal...

  20. DOE issues Finding of No Significant Impact for the Environmental...

    Office of Environmental Management (EM)

    the Environmental Assessment on Resumption of Transient Testing of Nuclear Fuels and Materials at Idaho National Laboratory DOE issues Finding of No Significant Impact for the...

  1. Sierra Geothermal's Key Find in Southern Nevada | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Geothermal's Key Find in Southern Nevada July 13, 2010 - 5:17pm Addthis Sierra Geothermal discovered temperatures hot enough for large-scale geothermal energy production at...

  2. EIS-0369: Record of Decision and Floodplain Statement of Findings...

    Broader source: Energy.gov (indexed) [DOE]

    Decision and Floodplain Statement of Findings Nevada Rail Alignment for the Disposal of Spent Nuclear Fuel and High-Level Radioactive Waste at Yucca Mountain, Nye County, Nevada...

  3. EA-1866: Final Environmental Assessment and Finding of No Significant...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Impact EA-1866: Final Environmental Assessment and Finding of No Significant Impact Argonne National Laboratory Modernization Planning This environmental assessment (EA) has...

  4. Frequency of Incidental Findings on Computed Tomography of Trauma Patients

    E-Print Network [OSTI]

    Devine, Alicia S; Jackson, Corinne S; Lyons, Lisa; Mason, Jon D

    2010-01-01T23:59:59.000Z

    findings on trauma computed tomography scans: experience atfindings in brain computed tomography scans of 3000 headfindings on chest computed tomography angiography performed

  5. Finding approximately rank-one submatrices with the nuclear norm ...

    E-Print Network [OSTI]

    2010-11-08T23:59:59.000Z

    We propose a convex optimization formulation with the nuclear norm and l1-norm to find a large approximately rank-one submatrix of a given nonnegative matrix ...

  6. Finding approximately rank-one submatrices with the nuclear norm ...

    E-Print Network [OSTI]

    Xuan Vinh Doan

    2010-11-08T23:59:59.000Z

    Nov 8, 2010 ... Abstract: We propose a convex optimization formulation with the nuclear norm and $\\ell_1$-norm to find a large approximately rank-one ...

  7. EA-1761: Finding of No Significant Impact and Floodplain Statement...

    Broader source: Energy.gov (indexed) [DOE]

    of No Significant Impact and Floodplain Statement of Findings Clemson University Wind Turbine Drivetrain Test Facility North Charleston, South Carolina The Department of Energy is...

  8. EA-1607: Finding of No Significant Impact | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    No Significant Impact EA-1607: Finding of No Significant Impact Disposition of DOE Excess Depleted Uranium, Natural Uranium, and Low-Enriched Uranium DOE is proposing the...

  9. Webinar: Wind-to-Hydrogen Cost Modeling and Project Findings

    Broader source: Energy.gov [DOE]

    Video recording and text version of the webinar titled, Wind-to-Hydrogen Cost Modeling and Project Findings, originally presented on January 17, 2013.

  10. EA-1956: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels DataDepartment of Energy Your Density Isn't Your Destiny:Revised Finding of No53197EFinding of NoFinding of7:4: FindingDraftFindingFinal56:

  11. EA-1994: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels DataDepartment of Energy Your Density Isn't Your Destiny:Revised Finding of No53197EFinding of NoFindingFIndingCalifornia94: Finding of No

  12. Finding Glass Kenton McHenry, Jean Ponce

    E-Print Network [OSTI]

    Forsyth, David

    Finding Glass Kenton McHenry, Jean Ponce Beckman Institute University of Illinois Urbana, IL 61801. This paper addresses the problem of finding glass ob- jects in images. Visual cues obtained by combining with the strong highlights typical of glass surfaces are used to train a hierarchy of classifiers, identify glass

  13. The Automation of Sound Reasoning and Successful Proof Finding

    E-Print Network [OSTI]

    Fitelson, Branden

    709 44 The Automation of Sound Reasoning and Successful Proof Finding LARRY WOS AND BRANDEN scientist naturally envisioned the automation of sound rea- soning ­ reasoning in which conclusions, and find proofs. But can such logical reasoning be fully automated? Can a single computer program

  14. On the Complexity of Finding Second-Best Abductive Explanations

    E-Print Network [OSTI]

    Liberatore, Paolo

    2012-01-01T23:59:59.000Z

    While looking for abductive explanations of a given set of manifestations, an ordering between possible solutions is often assumed. The complexity of finding/verifying optimal solutions is already known. In this paper we consider the computational complexity of finding second-best solutions. We consider different orderings, and consider also different possible definitions of what a second-best solution is.

  15. Uncorrected Proof Copy Finding Genes by Using Computational Tools 85

    E-Print Network [OSTI]

    Uncorrected Proof Copy Finding Genes by Using Computational Tools 85 Job: Plant Functional Genomics/Rev.02.03 Uncorrected Proof Copy #12;Uncorrected Proof Copy Finding Genes by Using Computational Tools 87 Computational Tools 89 Job: Plant Functional Genomics--Grotewold Compositor: Nettype Chapter: Chapter 6 Date: 3

  16. FINDING INFORMATION FOR TORONTO IN THE CENSUS OF CANADA 1901

    E-Print Network [OSTI]

    Haykin, Simon

    1 FINDING INFORMATION FOR TORONTO IN THE CENSUS OF CANADA 1901 (NOMINAL CENSUS) Go to 1901TorontoCensusDistrict,Sub-Districtandmicrofilmreelnumber To find information for an individual in the Census of Canada 1901, you need to know: - the Census Directory first, to get the street address. #12;2 Go to the Library andArchives Canada website for Census

  17. SUMMARY-BASED POINTER ANALYSIS FRAMEWORK FOR MODULAR BUG FINDING

    E-Print Network [OSTI]

    SUMMARY-BASED POINTER ANALYSIS FRAMEWORK FOR MODULAR BUG FINDING Marcio O. Buss Submitted of Arts and Sciences COLUMBIA UNIVERSITY 2007 #12;c 2007 Marcio O. Buss All Rights Reserved #12;ABSTRACT SUMMARY-BASED POINTER ANALYSIS FRAMEWORK FOR MODULAR BUG FINDING Marcio O. Buss Modern society

  18. Finding structural anomalies in graphs by means of quantum walks

    SciTech Connect (OSTI)

    Feldman, Edgar [Department of Mathematics, Graduate Center of the City University of New York, 365 Fifth Avenue, New York, New York 10016 (United States); Hillery, Mark; Zheng, Hongjun [Department of Physics, Hunter College of the City University of New York, 695 Park Avenue, New York, New York 10021 (United States); Lee, Hai-Woong [Department of Physics, Korea Advanced Institute of Science and Technology, Daejeon 305-701 (Korea, Republic of); Reitzner, Daniel; Buzek, Vladimir [Research Center for Quantum Information, Slovak Academy of Sciences, Dubravska cesta 9, 845 11 Bratislava (Slovakia)

    2010-10-15T23:59:59.000Z

    We explore the possibility of using quantum walks on graphs to find structural anomalies, such as extra edges or loops, on a graph. We focus our attention on star graphs, whose edges are like spokes coming out of a central hub. If there are N spokes, we show that a quantum walk can find an extra edge connecting two of the spokes or a spoke with a loop on it in O({radical}(N)) steps. We initially find that if all except one of the spokes have loops, the walk will not find the spoke without a loop, but this can be fixed if we choose the phase with which the particle is reflected from the vertex without the loop. Consequently, quantum walks can, under some circumstances, be used to find structural anomalies in graphs.

  19. Regulating expression of cell and tissue-specific genes by modifying transcription

    SciTech Connect (OSTI)

    Beachy, Roger N; Dai, Shunhong

    2010-06-14T23:59:59.000Z

    Transcriptional regulation is the primary step to control gene expression, therefore function. Such regulation is achieved primarily via a combination of the activities of the promoter cis regulatory DNA elements and trans regulatory proteins that function through binding to these DNA elements. Rice bZIP transcription factors RF2a, RF2b and RLP1 play key roles in regulating the activity of a vascular tissue specific promoter isolated from Rice Tungro Bacilliform Virus (RTBV), through their interactions with the Box II essential cis element located in the promoter (Dai et al., 2006., Dai et al., 2004., Yin et al., 1997). RF2a, RF2b and RLP1 possess multiple regulatory domains. Functional characterization reveals that those domains can activate or repress the activity of the RTBV promoter. It is equally as important to recognize that these proteins control plant development by regulating differentiation and/or function of the vascular tissues. Studies of transcriptional regulation of the RTBV promoter by this group of bZIP proteins will not only provide insights about gene expression in the vascular tissue, but also insights about general mechanisms of transcription activation and repression. The knowledge gained from this research will also enable us to develop a well-described set of tools that can be used to control expression of multiple genes in transgenic plants. We have proposed characterize the function domains of RF2a, RF2b and RLP1 and explore the biological function of the transcription repressor RLP1.

  20. Stochasticity and traffic jams in the transcription of ribosomal RNA: Intriguing role of termination and antitermination

    E-Print Network [OSTI]

    Stefan Klumpp; Terence Hwa

    2008-11-19T23:59:59.000Z

    In fast growing bacteria, ribosomal RNA (rRNA) is required to be transcribed at very high rates to sustain the high cellular demand on ribosome synthesis. This results in dense traffic of RNA polymerases (RNAP). We developed a stochastic model, integrating results of single-molecule and quantitative in vivo studies of E. coli, to evaluate the quantitative effect of pausing, termination, and antitermination on rRNA transcription. Our calculations reveal that in dense RNAP traffic, spontaneous pausing of RNAP can lead to severe "traffic jams", as manifested in the broad distribution of inter-RNAP distances and can be a major factor limiting transcription and hence growth. Our results suggest the suppression of these pauses by the ribosomal antitermination complex to be essential at fast growth. Moreover, unsuppressed pausing by even a few non-antiterminated RNAPs can already reduce transcription drastically under dense traffic. However, the termination factor Rho can remove the non-antiterminated RNAPs and restore fast transcription. The results thus suggest an intriguing role by Rho to enhance rather than attenuate rRNA transcription.

  1. How the DNA sequence affects the Hill curve of transcriptional response

    E-Print Network [OSTI]

    M. Sheinman; Y. Kafri

    2011-11-16T23:59:59.000Z

    The Hill coefficient is often used as a direct measure of the cooperativity of binding processes. It is an essential tool for probing properties of reactions in many biochemical systems. Here we analyze existing experimental data and demonstrate that the Hill coefficient characterizing the binding of transcription factors to their cognate sites can in fact be larger than one -- the standard indication of cooperativity -- even in the absence of any standard cooperative binding mechanism. By studying the problem analytically, we demonstrate that this effect occurs due to the disordered binding energy of the transcription factor to the DNA molecule and the steric interactions between the different copies of the transcription factor. We show that the enhanced Hill coefficient implies a significant reduction in the number of copies of the transcription factors which is needed to occupy a cognate site and, in many cases, can explain existing estimates for numbers of the transcription factors in cells. The mechanism is general and should be applicable to other biological recognition processes.

  2. EA-1313: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels DataDepartment of Energy Your Density Isn't Your Destiny:Revised Finding of No53197E TDrew Bittner AboutImpactFinal39: Finding01: Finding3:

  3. EA-1628: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels DataDepartment of Energy Your Density Isn't Your Destiny:Revised Finding of No53197E TDrew Bittner56:1: MitigationEA-161Finding of162Finding

  4. EA-1673: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels DataDepartment of Energy Your Density Isn't Your Destiny:Revised Finding of No53197E TDrew Bittner56:1:4: Finding of165 NP8:1670: Finding

  5. EA-1678: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels DataDepartment of Energy Your Density Isn't Your Destiny:Revised Finding of No53197E TDrew Bittner56:1:4: Finding of165 NP8:1670:7:Finding

  6. EA-1752: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels DataDepartment of Energy Your Density Isn't Your Destiny:Revised Finding of No53197E TDrewFinding of No Significant Impact6:EA-1752: Finding

  7. EA-1775: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels DataDepartment of Energy Your Density Isn't Your Destiny:Revised Finding of No53197E TDrewFinding of NoFinal: Finding of No

  8. EA-1969: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels DataDepartment of Energy Your Density Isn't Your Destiny:Revised Finding of No53197EFinding of NoFindingFInding of NoEA-1968: Final9:

  9. atypical imaging findings: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    evaluate our summaries with a new protocol that tests for representativeness 19 Review Gout Imaging of gout: findings and utility CiteSeer Summary: Imaging is a helpful tool for...

  10. Dietetic Internship Find out more about NIU's Dietetic Internship Program

    E-Print Network [OSTI]

    Kostic, Milivoje M.

    Dietetic Internship OPEN HOUSE Find out more about NIU's Dietetic Internship Program Tuesday, 2013 Nancy T. Prange, MS,RD,LDN Director, Dietetic Internship (815) 753-6386 nprange@niu.edu If you

  11. Researchers use machine learning to find useful structural properties...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    using ORNL's Compute and Data Environment for Science (CADES) to analyze large volumes of neutron and x-ray scattering data to find and identify these defects-a first step to...

  12. Finding All Valid Hand Configurations for a Given Precision Grasp

    E-Print Network [OSTI]

    Ros, LluĂ­s

    - Normal on contact point Formulation Robot hand MA-I mounted on a robot arm Test cases Find all Iteration of 2 basic steps The configuration of the robot arm has a closed-form solution Dimension

  13. adult women findings: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    women, and of which women are at risk, should assist in the planning of services for abuse prevention and treatment of the health consequences of abuse. Key Findings: The...

  14. EA-1642: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    EA-1642: Finding of No Significant Impact Design and Construction of an Early Lead Mini-Fischer-Tropsch Refinery at the University of Kentucky Center for Applied Energy Research...

  15. Finding Large Aperture Fractures in Geothermal Resource Areas...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    A Three-Component Long-Offset Surface Seismic Survey, PSInSAR, and Kinematic Analysis Finding Large Aperture Fractures in Geothermal Resource Areas Using A Three-Component...

  16. IQCJ-SCHIP1, a novel fusion transcript encoding a calmodulin-binding IQ motif protein

    SciTech Connect (OSTI)

    Kwasnicka-Crawford, Dorota A. [Department of Genetics and Genomic Biology, Hospital for Sick Children, Toronto, Ont. (Canada) and Department of Kinesiology and Health Science, Faculty of Health, York University, 346 Norman Bethune College, 4700 Keele Street, Ont., Canada M3J-1P3 (Canada)]. E-mail: dakc@yorku.ca; Carson, Andrew R. [Department of Genetics and Genomic Biology, Hospital for Sick Children, Toronto, Ont. (Canada); Scherer, Stephen W. [Department of Genetics and Genomic Biology, Hospital for Sick Children, Toronto, Ont. (Canada); Department of Molecular and Medical Genetics, University of Toronto, Ont. (Canada)

    2006-12-01T23:59:59.000Z

    The existence of transcripts that span two adjacent, independent genes is considered rare in the human genome. This study characterizes a novel human fusion gene named IQCJ-SCHIP1. IQCJ-SCHIP1 is the longest isoform of a complex transcriptional unit that bridges two separate genes that encode distinct proteins, IQCJ, a novel IQ motif containing protein and SCHIP1, a schwannomin interacting protein that has been previously shown to interact with the Neurofibromatosis type 2 (NF2) protein. IQCJ-SCHIP1 is located on the chromosome 3q25 and comprises a 1692-bp transcript encompassing 11 exons spanning 828 kb of the genomic DNA. We show that IQCJ-SCHIP1 mRNA is highly expressed in the brain. Protein encoded by the IQCJ-SCHIP1 gene was localized to cytoplasm and actin-rich regions and in differentiated PC12 cells was also seen in neurite extensions.

  17. Characterization and Transcription of Arsenic Respiration and Resistance Genes During In Situ Uranium Bioremediation

    SciTech Connect (OSTI)

    Giloteaux, L.; Holmes, Dawn E.; Williams, Kenneth H.; Wrighton, Kelly C.; Wilkins, Michael J.; Montgomery, Alison P.; Smith, Jessica A.; Orellana, Roberto; Thompson, Courtney A.; Roper, Thomas J.; Long, Philip E.; Lovley, Derek R.

    2013-02-04T23:59:59.000Z

    The possibility of arsenic release and the potential role of Geobacter in arsenic biogeochemistry during in situ uranium bioremediation was investigated because increased availability of organic matter has been associated with substantial releases of arsenic in other subsurface environments. In a field experiment conducted at the Rifle, CO study site, groundwater arsenic concentrations increased when acetate was added. The number of transcripts from arrA, which codes for the alpha subunit of dissimilatory As(V) reductase, and acr3, which codes for the arsenic pump protein Acr3, were determined with quantitative RT-PCR. Most of the arrA (> 60%) and acr3-1 (> 90%) sequences that were recovered were most similar to Geobacter species, while the majority of acr3-2 (>50%) sequences were most closely related to Rhodoferax ferrireducens. Analysis of transcript abundance demonstrated that transcription of acr3-1 by the subsurface Geobacter community was correlated with arsenic concentrations in the groundwater. In contrast, Geobacter arrA transcript numbers lagged behind the major arsenic release and remained high even after arsenic concentrations declined. This suggested that factors other than As(V) availability regulated transcription of arrA in situ even though the presence of As(V) increased transcription of arrA in cultures of G. lovleyi, which was capable of As(V) reduction. These results demonstrate that subsurface Geobacter species can tightly regulate their physiological response to changes in groundwater arsenic concentrations. The transcriptomic approach developed here should be useful for the study of a diversity of other environments in which Geobacter species are considered to have an important influence on arsenic biogeochemistry.

  18. Molecular genetic analysis of activation-tagged transcription factors thought to be involved in photomorphogenesis

    SciTech Connect (OSTI)

    Neff, Michael M.

    2011-06-23T23:59:59.000Z

    This is a final report for Department of Energy Grant No. DE-FG02-08ER15927 entitled “Molecular Genetic Analysis of Activation-Tagged Transcription Factors Thought to be Involved in Photomorphogenesis”. Based on our preliminary photobiological and genetic analysis of the sob1-D mutant, we hypothesized that OBP3 is a transcription factor involved in both phytochrome and cryptochrome-mediated signal transduction. In addition, we hypothesized that OBP3 is involved in auxin signaling and root development. Based on our preliminary photobiological and genetic analysis of the sob2-D mutant, we also hypothesized that a related gene, LEP, is involved in hormone signaling and seedling development.

  19. Finding Solutions to Solar's Soft Cost Dilemma | Department of Energy

    Energy Savers [EERE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directed off Energy.gov. Are you sure you want toworldPowerHome| Department of Energy Find EnergyFinding

  20. Finding a Career in Energy Efficiency | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels DataDepartment of Energy Your Density Isn't YourTransport inEnergy June 6-7, 2013 MeetingEA # 1440EnergyThree-ComponentFindingFinding a

  1. EA-1239: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels DataDepartment of Energy Your Density Isn't Your Destiny:Revised Finding of No53197E TDrew Bittner AboutImpactFinal39: Finding of No

  2. EA-1331: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels DataDepartment of Energy Your Density Isn't Your Destiny:Revised Finding of No53197E TDrew Bittner AboutImpactFinal39: Finding01:

  3. EA-1593: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels DataDepartment of Energy Your Density Isn't Your Destiny:Revised Finding of No53197E TDrew Bittner56:1: Mitigation Action3: Finding of No

  4. EA-1937: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels DataDepartment of Energy Your Density Isn't Your Destiny:Revised Finding of No53197EFinding of NoFinding of7: Draft Environmental

  5. EA-1796: Finding of No Significant Impact | Department of Energy

    Energy Savers [EERE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directed off Energy.gov. Are you0andEnergyGlobal NuclearofCommunication |DoesFinding of NoFinding of No

  6. EA-1868: Finding of No Significant Impact | Department of Energy

    Energy Savers [EERE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directed off Energy.gov. Are you0andEnergyGlobal NuclearofCommunication |DoesFinding of68: Finding of No

  7. Reduction of DNA contamination in RNA samples for reverse transcription-polymerase chain reaction using selective precipitation by compaction agents.

    E-Print Network [OSTI]

    Fox, George

    Reduction of DNA contamination in RNA samples for reverse transcription-polymerase chain reaction by reverse transcription-polymerase chain reaction (RT-PCR) is DNA contamination, which can produce artifactually increased mRNA concentration. Current methods to eliminate contaminating DNA can compromise

  8. USING DNASE DIGESTION DATA TO ACCURATELY IDENTIFY TRANSCRIPTION FACTOR BINDING SITES

    E-Print Network [OSTI]

    Hartemink, Alexander

    USING DNASE DIGESTION DATA TO ACCURATELY IDENTIFY TRANSCRIPTION FACTOR BINDING SITES KAIXUAN LUO1. But methods combining DNase digestion data with TF binding specificity information could potentially be used on the genomic digestion prod- ucts of deoxyribonuclease I (DNase I, which we will simply call DNase) might

  9. Chk2 regulates transcription-independent p53-mediated apoptosis in response to DNA damage

    SciTech Connect (OSTI)

    Chen Chen [Department of Geriatric Research, National Institute for Longevity Sciences, National Center for Geriatrics and Gerontology, Obu, Aichi 474-8522 (Japan); Shimizu, Shigeomi [Department of Post-Genomics Diseases, Osaka University Medical School, Suita, Osaka 565-0871 (Japan); Tsujimoto, Yoshihide [Department of Post-Genomics Diseases, Osaka University Medical School, Suita, Osaka 565-0871 (Japan); Motoyama, Noboru [Department of Geriatric Research, National Institute for Longevity Sciences, National Center for Geriatrics and Gerontology, Obu, Aichi 474-8522 (Japan)]. E-mail: motoyama@nils.go.jp

    2005-07-29T23:59:59.000Z

    The tumor suppressor protein p53 plays a central role in the induction of apoptosis in response to genotoxic stress. The protein kinase Chk2 is an important regulator of p53 function in mammalian cells exposed to ionizing radiation (IR). Cells derived from Chk2-deficient mice are resistant to the induction of apoptosis by IR, and this resistance has been thought to be a result of the defective transcriptional activation of p53 target genes. It was recently shown, however, that p53 itself and histone H1.2 translocate to mitochondria and thereby induces apoptosis in a transcription-independent manner in response to IR. We have now examined whether Chk2 also regulates the transcription-independent induction of apoptosis by p53 and histone H1.2. The reduced ability of IR to induce p53 stabilization in Chk2-deficient thymocytes was associated with a marked impairment of p53 and histone H1 translocation to mitochondria. These results suggest that Chk2 regulates the transcription-independent mechanism of p53-mediated apoptosis by inducing stabilization of p53 in response to IR.

  10. Regulation of the Arf tumor suppressor by E2F transcription factors

    E-Print Network [OSTI]

    Iaquinta, Phillip John

    2007-01-01T23:59:59.000Z

    Effective tumor suppression requires the appropriate function of two major signaling pathways, the pRB-E2F growth-control pathway and the p53 stress-response pathway. Members of the E2F family of transcription factors are ...

  11. Laser zona drilling does not induce hsp70i transcription in blastomeres of eight-cell

    E-Print Network [OSTI]

    Wangh, Lawrence J.

    Laser zona drilling does not induce hsp70i transcription in blastomeres of eight-cell mouse embryos To assess whether zona drilling with a 1,480-nm laser induces heat shock in eight-cell embryos, we measured that had or had not been heated at 43°C. Unlike heating, laser zona drilling did not stimulate hsp70i

  12. Genome-Wide Identification of Transcriptional Start Sites in the Plant Pathogen Pseudomonas syringae pv. tomato

    E-Print Network [OSTI]

    Myers, Chris

    syringae pv. tomato str. DC3000 Melanie J. Filiatrault1,2 *, Paul V. Stodghill1,2 , Christopher R. Myers3 identified 59-ends of transcripts for the plant pathogen Pseudomonas syringae pv. tomato str. DC3000. Citation: Filiatrault MJ, Stodghill PV, Myers CR, Bronstein PA, Butcher BG, et al. (2011) Genome

  13. Post-transcriptional modifications of Ser15 Thr18 Ser20 Ser155

    E-Print Network [OSTI]

    Bedwell, David M.

    used · Dox(doxorubicin): a DNA-damaging agent that is known to stabilize p53 · STZ(streptozotocin): an O-GlacNacase inhibitor. Effiect of Dox/STZ treatment on accumulation and O-GlcNAcylation of p53 #12;3 Effiect of Dox/STZ treatment on accumulation and O-GlcNAcylation of p53 Transcriptional level? O

  14. Transcriptional Regulation of Germinal Center B and Plasma Cell Fates by Dynamical Control of IRF4

    E-Print Network [OSTI]

    Dinner, Aaron

    analyses have led to the formulation of a ``kinetic control'' model for the regulation of B cell-f results support a model of ``kinetic control'' in which signaling-induced dynamics of IRF4 in activated BImmunity Article Transcriptional Regulation of Germinal Center B and Plasma Cell Fates by Dynamical

  15. Comparative Genomics of Transcriptional Control in the Human Malaria Parasite Plasmodium falciparum

    E-Print Network [OSTI]

    Arnold, Jonathan

    Comparative Genomics of Transcriptional Control in the Human Malaria Parasite Plasmodium falciparum Richard M.R. Coulson,1,3 Neil Hall,2 and Christos A. Ouzounis1 1 Computational Genomics Group10 1SD, United Kingdom; 2 The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus

  16. A central integrator of transcription networks in plant stress and energy signalling

    E-Print Network [OSTI]

    Sheen, Jen

    are the principal solar energy converter sustaining life on Earth. Despite its fundamental importance, little to globally regulate plant metabolism, energy balance, growth and survival. In con- trast to the prevailingLETTERS A central integrator of transcription networks in plant stress and energy signalling Elena

  17. Global RNA Half-Life Analysis in Escherichia coli Reveals Positional Patterns of Transcript Degradation

    E-Print Network [OSTI]

    Church, George M.

    Degradation Douglas W. Selinger,1,3 Rini Mukherjee Saxena,2,3 Kevin J. Cheung,1 George M. Church,1 and Carsten to study global RNA degradation in wild-type Escherichia coli MG1655. RNA chemical half-lives were measured of operons degrade, on average, more quickly than the rest of the transcript, with stability increasing

  18. The Transcription Factor WIN1/SHN1 Regulates Cutin Biosynthesis in Arabidopsis thaliana W

    E-Print Network [OSTI]

    Pauly, Markus

    influence on its ability to protect the plant against various forms of biotic and abiotic stress. WAX INDUCER1 (WIN1) and related transcription factors have recently been shown to trigger wax production of genes associated with wax biosynthesis, suggesting that the regulation of cutin and wax production

  19. Structural and mechanistic insights into cooperative assembly of dimeric Notch transcription complexes

    SciTech Connect (OSTI)

    Arnett, Kelly L.; Hass, Matthew; McArthur, Debbie G.; Ilagan, Ma Xenia G.; Aster, Jon C.; Kopan, Raphael; Blacklow, Stephen C. (WU); (BWH); (DFCI)

    2010-11-12T23:59:59.000Z

    Ligand-induced proteolysis of Notch produces an intracellular effector domain that transduces essential signals by regulating the transcription of target genes. This function relies on the formation of transcriptional activation complexes that include intracellular Notch, a Mastermind co-activator and the transcription factor CSL bound to cognate DNA. These complexes form higher-order assemblies on paired, head-to-head CSL recognition sites. Here we report the X-ray structure of a dimeric human Notch1 transcription complex loaded on the paired site from the human HES1 promoter. The small interface between the Notch ankyrin domains could accommodate DNA bending and untwisting to allow a range of spacer lengths between the two sites. Cooperative dimerization occurred on the human and mouse Hes5 promoters at a sequence that diverged from the CSL-binding consensus at one of the sites. These studies reveal how promoter organizational features control cooperativity and, thus, the responsiveness of different promoters to Notch signaling.

  20. Paucity of chimeric gene-transposable element transcripts in the Drosophila melanogastergenome

    E-Print Network [OSTI]

    Lipatov, Mikhail; Lenkov, Kapa; Petrov, Dmitri A; Bergman, Casey M

    2005-11-12T23:59:59.000Z

    useful sequences to the host genome; or 3) they are nei- ther particularly deleterious nor particularly advantageous in comparison to the observed non-chimeric TE insertions in the genome.Page 5 of 18 (page number not for citation purposes) transcription...

  1. Understanding Gene Sequence Variation in the Context of Transcription Regulation in Yeast

    E-Print Network [OSTI]

    Shamir, Ron

    Understanding Gene Sequence Variation in the Context of Transcription Regulation in Yeast Irit Gat-Viks1. *, Renana Meller2. , Martin Kupiec3 , Ron Shamir2 1 Broad Institute of Harvard and Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America, 2 School of Computer Science

  2. Repression of the Inhibin -Subunit Gene by the Transcription Factor CCAAT/Enhancer-

    E-Print Network [OSTI]

    Mayo, Kelly E.

    (A.D.B., K.E.M.), Evanston, Illinois 60208; Reproductive Endocrine Unit, Massachusetts General Hospital, Harvard Medical School (A.M.), Boston, Massachusetts 02114; and Molecular Mechanisms Institute (E.S.), and Eukaryotic Transcriptional Regulation Section, Laboratory of Protein Dynamics

  3. Genome-wide in silico identification and analysis of cis natural antisense transcripts (cis-NATs)

    E-Print Network [OSTI]

    Liu, Xiaole Shirley

    ; Revised May 10, 2006; Accepted June 21, 2006 ABSTRACT We developed a fast, integrative pipeline to identify cis natural antisense transcripts (cis-NATs) at genome scale. The pipeline mapped mRNAs and ESTs loci (1). NATs have already been found to function at several levels of eukaryotic gene regulation

  4. The Detection of Thyroid Receptor-Mediated Transcriptional Activity in Treated Peter Littlehat, Jr.

    E-Print Network [OSTI]

    Fay, Noah

    The Detection of Thyroid Receptor-Mediated Transcriptional Activity in Treated Wastewater by Peter) and other trace organic compounds that are released to the environment in treated domestic wastewaters of the thyroid hormone compounds under the two conditions, (ii) screen and compare the responsiveness of treated

  5. Activin Modulates the Transcriptional Response of L T2 Cells to Gonadotropin-Releasing Hormone and

    E-Print Network [OSTI]

    Lawson, Mark A.

    the transcriptional response of L T2 gonadotrope cells to GnRH stimulation. Ac- tivin treatment alone altered stimulate the production of gonadal steroids essential for reproductive development and function (1, 2. In this study, we undertook expression profiling to determine how activin pre- treatment alters

  6. Arnaud et al. A dominant mutation in the gene encoding the erythroid transcription factor

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    causes a congenital dyserythropoietic anemia Lionel Arnaud,1, * Carole Saison,1 Virginie Helias,1 Nicole-29Dec2010 #12;Arnaud et al. 3 Summary The congenital dyserythropoietic anemias (CDAs) are inherited red negative effect on KLF1 transcriptional activity and unexpectedly abolishes the expression of the water

  7. Using deep sequencing to characterize the biophysical mechanism of a transcriptional regulatory sequence

    E-Print Network [OSTI]

    Using deep sequencing to characterize the biophysical mechanism of a transcriptional regulatory sequence Justin B. Kinneya,b,1,2 , Anand Murugana , Curtis G. Callan, Jr.a,c,2 , and Edward C. Coxd be revealed by a simple experiment in which a library of partially mutated regulatory sequences

  8. Brief Communication 1595 Post-transcriptional control of the Arabidopsis auxin efflux

    E-Print Network [OSTI]

    Wurtele, Eve Syrkin

    for Applied Genetics, University of Agricultural Sciences, Muthgasse 18, A-1190 Vienna, Austria. John InnesBrief Communication 1595 Post-transcriptional control of the Arabidopsis auxin efflux carrier EIR1-9822/00/$ ­ see front matter © 2000 Elsevier Science Ltd. All rights reserved. Results and discussion The tissue

  9. Zfh1, a somatic motor neuron transcription factor, regulates axon exit from the CNS

    E-Print Network [OSTI]

    Oregon, University of

    Zfh1, a somatic motor neuron transcription factor, regulates axon exit from the CNS Michael J 2006 Abstract Motor neurons are defined by their axon projections, which exit the CNS to innervate somatic or visceral musculature, yet remarkably little is known about how motor axons are programmed

  10. A Small Post-Translocation Energy Bias Aids Nucleotide Selection in T7 RNA Polymerase Transcription

    E-Print Network [OSTI]

    Oster, George

    A Small Post-Translocation Energy Bias Aids Nucleotide Selection in T7 RNA Polymerase Transcription a crucial role in nucleotide selection at pre-insertion. The facilitated translocation is carried out, and forming a locally stabilized post-translocation intermediate. Pre-insertion of an incoming nucleotide

  11. Prediction of Transcription Start Sites Based on Feature Selection Using AMOSA

    E-Print Network [OSTI]

    1 Prediction of Transcription Start Sites Based on Feature Selection Using AMOSA Xi Wang1 sites (TSSs) is a primary and important step. With the aim to improve the computational prediction are extracted. Effective feature selection can minimize the noise, improve the prediction accuracy, and also

  12. Transcription Journal Club -2013-2014 September 3 Tuesday 1PM Magda Potok Journal Club

    E-Print Network [OSTI]

    Simons, Jack

    Transcription Journal Club - 2013-2014 September 3 Tuesday 1PM Magda Potok Journal Club September 10 Tuesday 5PM Stephanie Cuhna Research Talk September 17 Tuesday 1PM Yixuan Guo Journal Club Talk October 08 Tuesday 1PM Chongil Yi Journal Club October 15 Tuesday 5PM Christopher Gregg Postdoc

  13. Transcription Factor E2-2 Is an Essential and Specific Regulator of

    E-Print Network [OSTI]

    Symington, Lorraine S.

    . The molecular control of PDC lineage specification has been poorly understood. We report that basic helix-Hopkins syndrome patients was associated with aberrant expression profile and impaired IFN response of the PDC. E2-2 directly activated multiple PDC-enriched genes, including transcription factors involved in PDC development

  14. Splitting of H3H4 tetramers at transcriptionally active genes undergoing dynamic histone exchange

    E-Print Network [OSTI]

    Splitting of H3­H4 tetramers at transcriptionally active genes undergoing dynamic histone exchange RNA polymerase II. It has been long believed that histone deposition involves stable H3­H4 tetramers cells have raised the idea that preexisting H3­H4 tetramers might split into dimers, resulting in mixed

  15. SeqVISTA: a new module of integrated computational tools for studying transcriptional regulation

    E-Print Network [OSTI]

    Weng, Zhiping

    SeqVISTA: a new module of integrated computational tools for studying transcriptional regulation Zhenjun Hu1 , Yutao Fu1 , Anason S. Halees1 , Szymon M. Kielbasa3 and Zhiping Weng1,2, * 1 Bioinformatics 02215 and 3 Institute for Theoretical Biology, Humboldt University Berlin, Invalidenstraße 43, D-10115

  16. FINDING ROWS OF PEOPLE IN GROUP IMAGES Andrew C. Gallagher

    E-Print Network [OSTI]

    Chen, Tsuhan

    People are among the most popular subjects in photography, and in many social settings, images of groupsFINDING ROWS OF PEOPLE IN GROUP IMAGES Andrew C. Gallagher Carnegie Mellon University Department of people are captured. People often arrange themselves in a very struc- tured manner in these group images

  17. Orientation finding using a grid based visual compass

    E-Print Network [OSTI]

    Visser, Arnoud

    Orientation finding using a grid based visual compass Georgios Methenitis Patrick M. de Kok Sander an extension of the model-based visual compass is presented, which can be updated contin- uously, allowing, a visual compass can be used [1, 4, 5]. Such a method estimates the robot's heading by comparing

  18. Finding Stress Patterns in Microprocessor Workloads Frederik Vandeputte Lieven Eeckhout

    E-Print Network [OSTI]

    Eeckhout, Lieven

    Finding Stress Patterns in Microprocessor Workloads Frederik Vandeputte Lieven Eeckhout Department-end embedded systems to high-end supercom- puters. Understanding the power characteristics of a microprocessor) that stress the microprocessor to its extreme power con- sumption. This paper closes the gap between these two

  19. 2012PrOgram 2 Find a Job or Internship

    E-Print Network [OSTI]

    Hone, James

    OF EVENTS 2012PrOgram Fall #12;2 Find a Job or Internship 4 all Students and alumni 9 all Students 2, 2012 Deadline to accept full-time offers from previous summer internships November 16, 2012 Deadline to accept full-time offers Summer Internship Search through OCr October 22, 2012 Employer

  20. Table of Contents 2 Find a Job or Internship

    E-Print Network [OSTI]

    Hone, James

    F 2 0 1 1 A L L #12;Table of Contents 2 Find a Job or Internship 4 All Students and Alumni 10 All begin November 18th Deadline to accept full-time offers Summer Internship Search through OCR November 2 to accept internship offers Employer Information Sessions Employer Information Sessions are hosted on campus

  1. Healthcare MBA Prepare to Find Healthcare Management Solutions

    E-Print Network [OSTI]

    Vertes, Akos

    .5 Macroeconomics for the Global Economy 1.5 International Management 1.5 Management of Information Systems 1 and Healthcare 1.5 Disaster Management in Healthcare Organizations 1.5 Quality and Performance Improvement 1Healthcare MBA onLine #12;Prepare to Find Healthcare Management Solutions Healthcare is changing

  2. Root Finding Methods 1 using pylab, numpy, sympy, and Sage

    E-Print Network [OSTI]

    Verschelde, Jan

    Software (MCS 507 L-13) Root Finding Methods 25 September 2013 2 / 45 #12;polynomials with ipython --pylab-15 -2.44249065e-15j, -2.22044605e-16 +0.00000000e+00j]) By default, all objects in ipython --pylab

  3. Instruction Guide Paying Vendors: Using the Find an Existing

    E-Print Network [OSTI]

    Watson, Craig A.

    . Enter a Voucher ID number to begin a search. For this example, enter "03036649". Click the Search of Florida, Gainesville, FL 32611 OVERVIEW Search for a voucher and retrieve the original voucher screen, click on Regular Entry. Click the Find an Existing Value tab. Example 1 Searching by Voucher ID 1

  4. Preliminary Findings from an Analysis of Building Energy Information System

    E-Print Network [OSTI]

    -based energy monitoring, web-based energy management linked to controls, demand response, and enterprise energyLBNL-2224E Preliminary Findings from an Analysis of Building Energy Information System Technologies of Building Energy Information System Technologies Jessica Granderson Mary Ann Piette Girish Ghatikar Phillip

  5. Findings from Seven Years of Field Performance Data for Automated

    E-Print Network [OSTI]

    in Commercial Buildings S. Kiliccote, M.A. Piette, J. Mathieu, K. Parrish Environmental Energy Technologies;1 Findings from Seven Years of Field Performance Data for Automated Demand Response in Commercial Buildings. It provides a summary of participation, DR strategies and incentives. Commercial buildings can reduce peak

  6. On Finding Narrow Passages with Probabilistic Roadmap Planners

    E-Print Network [OSTI]

    Pratt, Vaughan

    On Finding Narrow Passages with Probabilistic Roadmap Planners David Hsu, Stanford University University, Stanford, CA, USA A probabilistic roadmap is a network of simple paths connecting collision­free configurations obtained by sampling a robot's configuration space at random. Several probabilistic roadmap

  7. Find It. Delete It. Protect It. Information Technology Security Strategy

    E-Print Network [OSTI]

    Sheridan, Jennifer

    Find It. Delete It. Protect It. Information Technology Security Strategy Executive Summary The general proposed strategy is to optimize risk management for information security incrementally and over that security will be a process rather than project. Achievement of the goal, optimized risk management

  8. Finding a rational nomenclature for mesons and baryons

    SciTech Connect (OSTI)

    Barnett, R.M.; Cahn, R.N.; Gidal, G.; Rittenberg, A.; Trippe, T.G.; Wohl, C.G.; Yost, G.P.; Porter, F.C.; Hernandez, J.J.; Montanet, L.

    1985-05-01T23:59:59.000Z

    A history of the Particle Data Group's efforts to find a rational and systematic convention for naming mesons and baryons is given. Several versions of our proposal are reviewed, and name changes which would occur are summarized. Some of the mail we have received is described. We hope to stimulate additional feedback.

  9. Finding and Mending Barrier Gaps in Wireless Sensor Networks

    E-Print Network [OSTI]

    Liu, Benyuan

    Finding and Mending Barrier Gaps in Wireless Sensor Networks Anwar Saipulla Benyuan Liu Jie Wang--Constructing sensing barriers using wireless sensor networks has important applications in military operations results show that our algorithms can effectively improve the barrier coverage of a wireless sensor network

  10. Finding hydrocarbons in the classroom using "free" seismic interpretation software

    E-Print Network [OSTI]

    Finding hydrocarbons in the classroom using "free" seismic interpretation software WAYNE D Technological Univer- sity, we recently introduced a new course in seismic processing and interpretation of this paper is to pro- vide details of the class assignment in seismic interpretation, and to encourage

  11. Allinea DDT: Your Partner in Finding Debugged Paths on Mira

    E-Print Network [OSTI]

    Kemner, Ken

    Allinea DDT: Your Partner in Finding Debugged Paths on Mira Ian Lumb Senior! #12;BG /P Case Study: Debugging Process Reproduced the crash Ran Allinea DDT in offline mode ­ MPI implementation bug? ­ Memory bug? Ran Allinea DDT in offline mode again Memory debugging enabled

  12. Finding Environment Guarantees Marsha Chechik, Mihaela Gheorghiu, Arie Gurfinkel

    E-Print Network [OSTI]

    Chechik, Marsha

    Finding Environment Guarantees Marsha Chechik, Mihaela Gheorghiu, Arie Gurfinkel University a software component, a model of the environ- ment in which that component is supposed to run is constructed of the environment model. In this paper, we identify and formalize a problem related to environment models

  13. ExtraSolar Planets Finding Extrasolar Planets. I

    E-Print Network [OSTI]

    Walter, Frederick M.

    Planets. IV Most planets have been found by Doppler Wobble (radial velocity variations). This selects/m* rp (center of mass) V* = 2 mp/m* / (rp)1/2 V¤ = 2 cm/s; V¤J = 3 m/s #12;Doppler Effect Emission from The Doppler Effect applied to pulse arrival times. Applicable to pulsar planets #12;Finding Extrasolar Planets

  14. Finding the needle in the haystack: Algorithms for conformational optimization

    SciTech Connect (OSTI)

    Andricioaei, I.; Straub, J.E.

    1996-09-01T23:59:59.000Z

    Algorithms are given for comformational optimization of proteins. The protein folding problems is regarded as a problem of global energy mimimization. Since proteins have hundreds of atoms, finding the lowest-energy conformation in a many-dimensional configuration space becomes a computationally demanding problem.{copyright} {ital American Institute of Physics.}

  15. Biofuels, biodiversity, and people: Understanding the conflicts and finding opportunities

    E-Print Network [OSTI]

    Review Biofuels, biodiversity, and people: Understanding the conflicts and finding opportunities interests in biofuels. Biofuels are viewed by many policy makers as a key to reducing reliance on foreign concerns, and by reports questioning the rationale that biofuels substantially reduce carbon emissions. We

  16. Finding the ciliary beating pattern with optimal efficiency

    E-Print Network [OSTI]

    Osterman, Natan

    Finding the ciliary beating pattern with optimal efficiency Natan Ostermana,b and Andrej Vilfana, and approved July 20, 2011 (received for review May 17, 2011) We introduce a measure for energetic efficiency according to this criterion. Maximizing the efficiency of a single cilium leads to curly, often symmetric

  17. Find your winning position in the MRO game

    E-Print Network [OSTI]

    Keep them flying Find your winning position in the MRO game Aerospace and Defense IBM Institute currently holds an inventory of about US$44 billion and supports an active global fleet of nearly 17 percent of their total expenditures. (Fuel and labor are other big expenses.) 6 Airlines spend an average

  18. STAFF TRAVEL SURVEY 2006 KEY FINDINGS Survey introduction

    E-Print Network [OSTI]

    Brierley, Andrew

    routes. · Car users are most likely to be encouraged to car share if help is given in finding suitable car sharing partners. How do you usually travel to work? Almost half of all staff (46%) drive to work alone. Another 23% car share, giving a total of 70% staff using a car to travel to work. Comparing

  19. OPTIMAL ALGORITHM FOR FINDING DNA MOTIFS WITH NUCLEOTIDE ADJACENT DEPENDENCY

    E-Print Network [OSTI]

    Chin, Francis Y.L.

    OPTIMAL ALGORITHM FOR FINDING DNA MOTIFS WITH NUCLEOTIDE ADJACENT DEPENDENCY FRANCIS Y.L. CHIN that the occurrence of a nucleotide in a binding site is independent of other nucleotides. More complicated representations, such as HMM or regular expression, exist that can capture the nucleotide dependency

  20. Help to find the way March 20, 2008

    E-Print Network [OSTI]

    Toronto, University of

    Help to find the way March 20, 2008 SHAUNA REMPEL TORONTO STAR Name: Mike Wu. Age: 28. Program: Ph their memories when they become disoriented. "People with severe memory impairment have difficulty remembering." Software can program the personal digital assistant, or PDA, with instructions from family or caregivers

  1. Finding literature reviews WHAT IS A LITERATURE REVIEW?

    E-Print Network [OSTI]

    Ishida, Yuko

    already been done in the field to suggest future research directions or gaps in knowledge Green, Johnson the process of writing a good literature review. To find guides from many other libraries, search online Green BN, Johnson CD, Adams A. Writing narrative literature reviews for peer-reviewed journals: secrets

  2. Climatic consequences of nuclear war: new findings, 1985

    SciTech Connect (OSTI)

    Knox, J.B.

    1985-12-01T23:59:59.000Z

    The history of the discovery of nuclear winter was reviewed, and the findings of the TTAPS study summarized. A simulation of a moderate exchange with certain key uncertainties taken into account was then briefly reviewed to see what the corresponding climatic consequences are. 12 refs., 4 figs. (ACR)

  3. Finding Heavy Distinct Hitters in Data Streams Thomas Locher

    E-Print Network [OSTI]

    of a distributed denial of service (DDoS) attack, where many distinct entities target one or a small number traffic volume, there are certain DDoS attacks, e.g., TCP SYN flooding attacks, that is considerably harder. In this paper, we study the problem of finding all entities whose number of distinct

  4. Integrated cistromic and expression analysis of amplified NKX2-1 in lung adenocarcinoma identifies LMO3 as a functional transcriptional target

    E-Print Network [OSTI]

    Watanabe, Hideo

    The NKX2-1 transcription factor, a regulator of normal lung development, is the most significantly amplified gene in human lung adenocarcinoma. To study the transcriptional impact of NKX2-1 amplification, we generated an ...

  5. New technology for gas finding; How important has it been

    SciTech Connect (OSTI)

    Ellis, P.A. (Booz Allen and Hamilton, Dallas, TX (US))

    1991-09-30T23:59:59.000Z

    This paper reports that finding costs for natural gas in the U.S. were more than halved in real terms from 94{cents}/Mcf in 1983 to 44{cents} in 1989. A number of industry analysts and oil company executives recently have suggested that technology improvements contributed significantly to this improvement. This claim is an especially important one because its proponents often use it to support the view that the gas glut in the U.S. will persist. In this view, new technology will push finding costs lower and help sustain deliverability, further prolonging the bubble. Managers who are convinced of this position will want to invest in the people, software, and equipment that is supposed to be behind this improved performance. But they also are more likely to defer drilling and acquisitions of companies or producing leases to avoid adding to the supply overhang.

  6. New Algorithms for Finding Approximate Frequent Item Sets

    E-Print Network [OSTI]

    Berthold, Michael R.

    New Algorithms for Finding Approximate Frequent Item Sets Christian Borgelt1 , Christian Braune1,2 , Tobias K¨otter3 and Sonja Gr¨un4,5 1 European Centre for Soft Computing c/ Gonzalo Guti´errez Quir´os s/n.borgelt@softcomputing.es, christian.braune@st.ovgu.de, tobias.koetter@uni-konstanz.de, s.gruen@fz-juelich.de Abstract. In standard

  7. New way to predict plutonium Finding could lead to

    E-Print Network [OSTI]

    Savrasov, Sergej Y.

    New way to predict plutonium safety Finding could lead to improved storage of nuclear weapons of 2Science Front Page 2:14 PM ET Thursday, April 12, 2001 9/5/2003file://E:\\Homepages\\SavrasovHome\\Projects\\Research\\Plutonium, and Privacy Page 2 of 2Science Front Page 2:14 PM ET Thursday, April 12, 2001 9/5/2003file://E:\\Homepages\\SavrasovHome\\Projects\\Research\\Plutonium

  8. X-ray Crystal Structures Elucidate the Nucleotidyl Transfer Reaction of Transcript Initiation Using Two Nucleotides

    SciTech Connect (OSTI)

    M Gleghorn; E Davydova; R Basu; L Rothman-Denes; K Murakami

    2011-12-31T23:59:59.000Z

    We have determined the X-ray crystal structures of the pre- and postcatalytic forms of the initiation complex of bacteriophage N4 RNA polymerase that provide the complete set of atomic images depicting the process of transcript initiation by a single-subunit RNA polymerase. As observed during T7 RNA polymerase transcript elongation, substrate loading for the initiation process also drives a conformational change of the O helix, but only the correct base pairing between the +2 substrate and DNA base is able to complete the O-helix conformational transition. Substrate binding also facilitates catalytic metal binding that leads to alignment of the reactive groups of substrates for the nucleotidyl transfer reaction. Although all nucleic acid polymerases use two divalent metals for catalysis, they differ in the requirements and the timing of binding of each metal. In the case of bacteriophage RNA polymerase, we propose that catalytic metal binding is the last step before the nucleotidyl transfer reaction.

  9. Beyond position weight matrices: nucleotide correlations in transcription factor binding sites and their description

    E-Print Network [OSTI]

    Santolini, Marc; Hakim, Vincent

    2013-01-01T23:59:59.000Z

    The identification of transcription factor binding sites (TFBSs) on genomic DNA is of crucial importance for understanding and predicting regulatory elements in gene networks. TFBS motifs are commonly described by Position Weight Matrices (PWMs), in which each DNA base pair independently contributes to the transcription factor (TF) binding, despite mounting evidence of interdependence between base pairs positions. The recent availability of genome-wide data on TF-bound DNA regions offers the possibility to revisit this question in detail for TF binding {\\em in vivo}. Here, we use available fly and mouse ChIPseq data, and show that the independent model generally does not reproduce the observed statistics of TFBS, generalizing previous observations. We further show that TFBS description and predictability can be systematically improved by taking into account pairwise correlations in the TFBS via the principle of maximum entropy. The resulting pairwise interaction model is formally equivalent to the disordered ...

  10. Graph Coarsening for Path Finding in Cybersecurity Graphs

    SciTech Connect (OSTI)

    Hogan, Emilie A.; Johnson, John R.; Halappanavar, Mahantesh

    2013-01-01T23:59:59.000Z

    n the pass-the-hash attack, hackers repeatedly steal password hashes and move through a computer network with the goal of reaching a computer with high level administrative privileges. In this paper we apply graph coarsening in network graphs for the purpose of detecting hackers using this attack or assessing the risk level of the network's current state. We repeatedly take graph minors, which preserve the existence of paths in the graph, and take powers of the adjacency matrix to count the paths. This allows us to detect the existence of paths as well as find paths that have high risk of being used by adversaries.

  11. Transportation Energy Futures: Project Overview and Findings (Presentation)

    SciTech Connect (OSTI)

    Not Available

    2013-03-01T23:59:59.000Z

    The U.S. Department of Energy-sponsored Transportation Energy Futures (TEF) project examines how combining multiple strategies could reduce both GHG emissions and petroleum use by 80%. The project's primary objective was to help inform domestic decisions about transportation energy strategies, priorities, and investments, with an emphasis on previously underexplored opportunities related to energy efficiency and renewable energy in light-duty vehicles, non-light-duty vehicles, fuels, and transportation demand. This PowerPoint provides an overview of the project and its findings.

  12. Wood residuals find big uses in small pieces

    SciTech Connect (OSTI)

    Glenn, J.

    1996-12-01T23:59:59.000Z

    With a history of finding economic uses for leftovers, the wood industry explores sustainable options for creating higher value products. Years ago, companies saw the use - any use - of residues as a sound, economic business practice. Today, many companies are looking to go beyond low value products such as mulch, animal bedding and fuel, and market to higher value end users. Additionally, with so much material from the primary industries already accounted for, consumers of wood residue are in need of additional supply from sources such as secondary mills (furniture manufacturers, etc.), as wells as the C&D and MSW streams. This paper discusses these products and markets.

  13. EIS-0361: Record of Decision and Floodplain Statement of Findings |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels DataDepartment of Energy Your Density Isn't Your Destiny:Revised Finding of98-F,-SA-01:5-SA-01:Draftofto ConductDraft:Statement |Notice

  14. EIS-0365: Record of Decision and Floodplain Statement of Findings |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels DataDepartment of Energy Your Density Isn't Your Destiny:Revised Finding of98-F,-SA-01:5-SA-01:Draftoftoto Conduct Public Scoping

  15. EIS-0372: Record of Decision and Floodplain Statement of Findings |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels DataDepartment of Energy Your Density Isn't Your Destiny:Revised Finding of98-F,-SA-01:5-SA-01:Draftoftoto ConductDepartment of Energy

  16. EIS-0385: Record of Decision and Floodplain Statement of Findings |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels DataDepartment of Energy Your Density Isn't Your Destiny:Revised FindingDepartment of Energy : Notice ofRecordDepartment of Energy

  17. EIS-0389: Record of Decision and Floodplain Statement of Findings |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels DataDepartment of Energy Your Density Isn't Your Destiny:Revised FindingDepartment of Energy : NoticeStatement |FinalConduct

  18. EIS-0389: Record of Decision and Floodplain Statement of Findings |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels DataDepartment of Energy Your Density Isn't Your Destiny:Revised FindingDepartment of Energy : NoticeStatement |FinalConductDepartment

  19. EIS-0395: Record of Decision and Floodplain Statement of Findings |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels DataDepartment of Energy Your Density Isn't Your Destiny:Revised FindingDepartment of Energy :to Extend ScopingPublic| Department

  20. EIS-0395: Record of Decision and Floodplain Statement of Findings |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels DataDepartment of Energy Your Density Isn't Your Destiny:Revised FindingDepartment of Energy :to Extend ScopingPublic|

  1. EIS-0456: Record of Decision and Floodplain Statement of Findings |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels DataDepartment of Energy Your Density Isn't Your Destiny:Revised FindingDepartment ofStatementStatementRecord ofDepartment of Energy

  2. Find Funding | U.S. DOE Office of Science (SC)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742EnergyOnItem NotEnergy,ARMFormsGasReleaseSpeechesHall ATours, Programs and EventsFiber LasersFind

  3. Neutrons find "missing" magnetism of plutonium

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of Science (SC)Integrated CodesTransparency VisitSilverNephelineNeural probeNeutrons find

  4. EA-1728: Finding of No Significant Impact | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742Energy China U.S.ContaminationJuly 2011D APPENDIXKahuku Wind Power, LLC,Finding of No Significant

  5. EA-1729: Finding of No Significant Impact | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742Energy China U.S.ContaminationJuly 2011D APPENDIXKahuku Wind Power, LLC,Finding of No

  6. EA-1731: Finding of No Significant Impact | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742Energy China U.S.ContaminationJuly 2011D APPENDIXKahuku Wind Power, LLC,Finding of

  7. EA-1732: Finding of No Significant Impact | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742Energy China U.S.ContaminationJuly 2011D APPENDIXKahuku Wind Power, LLC,Finding ofThisNext

  8. EA-1732: Finding of No Significant Impact | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742Energy China U.S.ContaminationJuly 2011D APPENDIXKahuku Wind Power, LLC,Finding

  9. EA-1733: Finding of No Significant Impact | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742Energy China U.S.ContaminationJuly 2011D APPENDIXKahuku Wind Power, LLC,FindingMonroe, LACalpine

  10. EA-1736: Finding of No Significant Impact | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742Energy China U.S.ContaminationJuly 2011D APPENDIXKahuku Wind Power, LLC,FindingMonroe,

  11. EA-1737: Finding of No Significant Impact | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742Energy China U.S.ContaminationJuly 2011D APPENDIXKahuku Wind Power, LLC,FindingMonroe,Los

  12. EA-1739: Finding of No Significant Impact | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742Energy China U.S.ContaminationJuly 2011D APPENDIXKahuku Wind Power, LLC,FindingMonroe,LosProject

  13. EA-1762: Finding of No Significant Impact | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742Energy China U.S.ContaminationJuly 2011D APPENDIXKahuku WindEnergy ThisFindings |

  14. EA-1763: Finding of No Significant Impact | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742Energy China U.S.ContaminationJuly 2011D APPENDIXKahuku WindEnergy ThisFindings |Geothermal

  15. EA-1767: Finding of No Significant Impact | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742Energy China U.S.ContaminationJuly 2011D APPENDIXKahuku WindEnergy ThisFindingsVirginia State

  16. EA-1769: Final Environmental Assessment and Finding of No Significant

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742Energy China U.S.ContaminationJuly 2011D APPENDIXKahuku WindEnergy ThisFindingsVirginia

  17. EA-1901: Finding of No Significant Impact | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742Energy China U.S.ContaminationJuly 2011DDelphi Automotive Systems,DOEOldThisFindingBased on the

  18. EA-1903: Finding of No Significant Impact | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742Energy China U.S.ContaminationJuly 2011DDelphi Automotive Systems,DOEOldThisFindingBasedbyBased

  19. EA-1925: Finding of No Significant Impact | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742Energy China U.S.ContaminationJuly 2011DDelphiFEA-2013.pdfBased on the final EA, DOE finds that

  20. How to Find Ames Laboratory | The Ames Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of Science (SC) EnvironmentalGyroSolé(tm) HarmonicbetandEnergy 2010a Wind TurbineContactFind

  1. FINDING OF NO SIGNIFICANT IMPACT FOR THE LINAC COHERENT LIGHT

    Office of Environmental Management (EM)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742 33 1112011AT&T,OfficeEnd of Year 2010SaltInstrumentation andFEFACILITY1 - In the6FINDING

  2. Find Financing for Energy Efficiency Upgrades | Department of Energy

    Energy Savers [EERE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directed offOCHCO2: FinalOffers New Training on Energy6 FederalofE:Financing MechanismsFinancing andFind

  3. Finding a Career in Energy Efficiency | Department of Energy

    Energy Savers [EERE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directed offOCHCO2: FinalOffers New Training on Energy6 FederalofE:FinancingFinding a Career in Energy

  4. Floodplain Assessment and Statement of Findings | Department of Energy

    Energy Savers [EERE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directed offOCHCO2: FinalOffers3.pdf0-45.pdf Flash2010-45.pdfFlash2011-43and Statement of Findings Floodplain

  5. EA-1983: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE: Alternative FuelsNovember 13, 2014ContributingDOEDepartment1-E Wholesale PowerAboutFinalFinding of

  6. Portsmouth Integration Director's Final Findings and Order | Department of

    Office of Environmental Management (EM)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742 33 1112011 Strategic2 OPAM615_CostNSAR - T enAmount for IndividualEnergyFindings

  7. Community Wind Handbook/Find an Installer | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directedAnnual Siteof EnergyInnovationin UrbanCityCoatedCommunity Electric Coop JumpProject |Find an

  8. UDAC Findings and Recommendations 2014 | Department of Energy

    Office of Environmental Management (EM)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742 33Frequently20,000 Russian Nuclear Warheads into Fuel for U.S. Electricity | Department of1Findings

  9. EA-0984: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels DataDepartment of Energy Your Density Isn't Your Destiny:Revised Finding of No53197E TDrew Bittner AboutImpact Statement |70:EA-098-E84:

  10. EA-1440: Finding of No Signficant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels DataDepartment of Energy Your Density Isn't Your Destiny:Revised Finding of No53197E TDrew Bittner AboutImpactFinal39:3:2: Final-S1:S2::

  11. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page onYouTube YouTube Note: Since the YouTube|6721Energy 3_adv_battery.pdfFerrinMarket SegmentsPortfoliosFinding a

  12. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page onYouTube YouTube Note: Since the YouTube|6721Energy 3_adv_battery.pdfFerrinMarket SegmentsPortfoliosFinding aDecember

  13. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page onYouTube YouTube Note: Since the YouTube|6721Energy 3_adv_battery.pdfFerrinMarket SegmentsPortfoliosFinding

  14. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page onYouTube YouTube Note: Since the YouTube|6721Energy 3_adv_battery.pdfFerrinMarket SegmentsPortfoliosFindingAugust 11,

  15. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page onYouTube YouTube Note: Since the YouTube|6721Energy 3_adv_battery.pdfFerrinMarket SegmentsPortfoliosFindingAugust

  16. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page onYouTube YouTube Note: Since the YouTube|6721Energy 3_adv_battery.pdfFerrinMarketAugust 13, 2010 EA-1758: Finding of

  17. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page onYouTube YouTube Note: Since the YouTube|6721Energy 3_adv_battery.pdfFerrinMarketAugust 13, 2010 EA-1758: Finding

  18. EA-1426: Finding of No Significant Impact | Department of Energy

    Energy Savers [EERE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directed off Energy.gov. Are you0andEnergyGlobal NuclearofCommunication |Does YourDr.36 EA26: Finding of

  19. EA-1548: Finding of No Significant Impact | Department of Energy

    Energy Savers [EERE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directed off Energy.gov. Are you0andEnergyGlobal NuclearofCommunication |Does YourDr.36 EA26:48: Finding

  20. EA-1752: Finding of No Significant Impact | Department of Energy

    Energy Savers [EERE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directed off Energy.gov. Are you0andEnergyGlobal NuclearofCommunication |DoesFinding of No Significant

  1. EA-1795: Floodplain Statement of Findings | Department of Energy

    Energy Savers [EERE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directed off Energy.gov. Are you0andEnergyGlobal NuclearofCommunication |DoesFinding of No

  2. EA-1937: Finding of No Significant Impact | Department of Energy

    Energy Savers [EERE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directed off Energy.gov. Are you0andEnergyGlobal NuclearofCommunicationMitigation Action7: Finding of No

  3. Research Finds Vitamin D Deficiency Affects Bone Quality

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of Scienceand Requirements Recently ApprovedReliabilityPrincipalResearch Finds Vitamin D Deficiency

  4. Title 16 USC 1600 Congressional Findings | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to:Ezfeedflag JumpID-f <MaintainedInformationThePty Ltd Jump to:OffshoreOpenI WindFindings

  5. Research Finds Vitamin D Deficiency Affects Bone Quality

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level:Energy: Grid Integration Redefining What's PossibleRadiation Protection Technical s o Freiberge s 3 % A PB 2 7 7 2ResearchAreasResearch Finds

  6. EA-1376: Finding of No Significant Impact | Department of Energy

    Energy Savers [EERE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directed offOCHCO OverviewAttachments461-93Do-It-YourselfDucts Sealing Using236: FinalEA-1338:FinalFinding of

  7. Finding Engineering Inspiration at GE | GE Global Research

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of Science (SC) Environmental Assessments (EA)Budget »TraveleBooks Find eBooks Many of theseGetting

  8. Finding Hidden Oil and Gas Reserves Project at NERSC

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of Science (SC) Environmental Assessments (EA)Budget »TraveleBooks Find eBooks Many of

  9. Finding renewable energy materials using one screensaver at a time:

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of Science (SC) Environmental Assessments (EA)Budget »TraveleBooks Find eBooks Many

  10. NREL Study Finds Barotrauma Not Guilty | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page onYouTube YouTube Note: Since the.pdfBreaking ofOilNEW HAMPSHIRE BUILDS OFF BEACON COMMUNITIESRenewable EnergyStudy Finds

  11. How yeast re-programmes its transcriptional profile in response to different nutrient impulses

    E-Print Network [OSTI]

    Dikicioglu, Duygu; Karabekmez, Erkan; Rash, Bharat; Pir, Pinar; Kirdar, Betul; Oliver, Stephen G

    2011-09-25T23:59:59.000Z

    * Correspondence: sgo24@cam.ac.uk 1Department of Chemical Engineering, Bogazici University, Bebek 34342, Istanbul, Turkey Full list of author information is available at the end of the article Dikicioglu et al. BMC Systems Biology 2011, 5:148 http... of an increase in nutrient sup- ply. Ronen and Botstein [4] have investigated the transi- ent transcriptional response to switching carbon sources between galactose and glucose and concluded that experimental designs that involve short-term transient...

  12. IKK{epsilon} modulates RSV-induced NF-{kappa}B-dependent gene transcription

    SciTech Connect (OSTI)

    Bao Xiaoyong; Indukuri, Hemalatha; Liu Tianshuang; Liao Suiling [Department of Pediatrics, University of Texas Medical Branch, Galveston, TX (United States); Tian, Bing; Brasier, Allan R. [Department of Internal Medicine, University of Texas Medical Branch, Galveston, TX (United States); Garofalo, Roberto P. [Department of Pediatrics, University of Texas Medical Branch, Galveston, TX (United States); Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX (United States); Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, TX (United States); Casola, Antonella, E-mail: ancasola@utmb.ed [Department of Pediatrics, University of Texas Medical Branch, Galveston, TX (United States); Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX (United States); Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, TX (United States)

    2010-12-20T23:59:59.000Z

    Respiratory syncytial virus (RSV), a negative-strand RNA virus, is the most common cause of epidemic respiratory disease in infants and young children. RSV infection of airway epithelial cells induces the expression of immune/inflammatory genes through the activation of a subset of transcription factors, including Nuclear Factor-{kappa}B (NF-{kappa}B). In this study we have investigated the role of the non canonical I{kappa}B kinase (IKK){epsilon} in modulating RSV-induced NF-{kappa}B activation. Our results show that inhibition of IKK{epsilon} activation results in significant impairment of viral-induced NF-{kappa}B-dependent gene expression, through a reduction in NF-{kappa}B transcriptional activity, without changes in nuclear translocation or DNA-binding activity. Absence of IKK{epsilon} results in a significant decrease of RSV-induced NF-{kappa}B phosphorylation on serine 536, a post-translational modification important for RSV-induced NF-{kappa}B-dependent gene expression, known to regulate NF-{kappa}B transcriptional activity without affecting nuclear translocation. This study identifies a novel mechanism by which IKK{epsilon} regulates viral-induced cellular signaling.

  13. Finding Text Information in the Ocean of Electronic Documents

    SciTech Connect (OSTI)

    Medvick, Patricia A.; Calapristi, Augustin J.

    2003-02-05T23:59:59.000Z

    Information management in natural resources has become an overwhelming task. A massive amount of electronic documents and data is now available for creating informed decisions. The problem is finding the relevant information to support the decision-making process. Determining gaps in knowledge in order to propose new studies or to determine which proposals to fund for maximum potential is a time-consuming and difficult task. Additionally, available data stores are increasing in complexity; they now may include not only text and numerical data, but also images, sounds, and video recordings. Information visualization specialists at Pacific Northwest National Laboratory (PNNL) have software tools for exploring electronic data stores and for discovering and exploiting relationships within data sets. These provide capabilities for unstructured text explorations, the use of data signatures (a compact format for the essence of a set of scientific data) for visualization (Wong et al 2000), visualizations for multiple query results (Havre et al. 2001), and others (http://www.pnl.gov/infoviz ). We will focus on IN-SPIRE, a MS Windows vision of PNNL’s SPIRE (Spatial Paradigm for Information Retrieval and Exploration). IN-SPIRE was developed to assist information analysts find and discover information in huge masses of text documents.

  14. FINDING THE FIRST COSMIC EXPLOSIONS. I. PAIR-INSTABILITY SUPERNOVAE

    SciTech Connect (OSTI)

    Whalen, Daniel J.; Smidt, Joseph; Lovekin, C. C. [T-2, Los Alamos National Laboratory, Los Alamos, NM 87545 (United States); Even, Wesley; Fryer, Chris L. [CCS-2, Los Alamos National Laboratory, Los Alamos, NM 87545 (United States); Frey, Lucille H. [HPC-3, Los Alamos National Laboratory, Los Alamos, NM 87545 (United States); Johnson, Jarrett L.; Hungerford, Aimee L. [XTD-6, Los Alamos National Laboratory, Los Alamos, NM 87545 (United States); Stiavelli, Massimo [Space Telescope Science Institute, 3700 San Martin Drive, Baltimore, MD 21218 (United States); Holz, Daniel E. [Enrico Fermi Institute, Department of Physics, and Kavli Institute for Cosmological Physics, University of Chicago, Chicago, IL 60637 (United States); Heger, Alexander [Monash Centre for Astrophysics, Monash University, Victoria 3800 (Australia); Woosley, S. E. [Department of Astronomy and Astrophysics, UCSC, Santa Cruz, CA 95064 (United States)

    2013-11-10T23:59:59.000Z

    The first stars are the key to the formation of primitive galaxies, early cosmological reionization and chemical enrichment, and the origin of supermassive black holes. Unfortunately, in spite of their extreme luminosities, individual Population III (Pop III) stars will likely remain beyond the reach of direct observation for decades to come. However, their properties could be revealed by their supernova explosions, which may soon be detected by a new generation of near-IR (NIR) observatories such as JWST and WFIRST. We present light curves and spectra for Pop III pair-instability supernovae calculated with the Los Alamos radiation hydrodynamics code RAGE. Our numerical simulations account for the interaction of the blast with realistic circumstellar envelopes, the opacity of the envelope, and Lyman absorption by the neutral intergalactic medium at high redshift, all of which are crucial to computing the NIR signatures of the first cosmic explosions. We find that JWST will detect pair-instability supernovae out to z ?> 30, WFIRST will detect them in all-sky surveys out to z ? 15-20, and LSST and Pan-STARRS will find them at z ?< 7-8. The discovery of these ancient explosions will probe the first stellar populations and reveal the existence of primitive galaxies that might not otherwise have been detected.

  15. Testing the Accuracy of Redshift Space Group Finding Algorithms

    E-Print Network [OSTI]

    James J. Frederic

    1994-09-07T23:59:59.000Z

    Using simulated redshift surveys generated from a high resolution N-body cosmological structure simulation, we study algorithms used to identify groups of galaxies in redshift space. Two algorithms are investigated; both are friends-of-friends schemes with variable linking lengths in the radial and transverse dimensions. The chief difference between the algorithms is in the redshift linking length. The algorithm proposed by Huchra \\& Geller (1982) uses a generous linking length designed to find ``fingers of god'' while that of Nolthenius \\& White (1987) uses a smaller linking length to minimize contamination by projection. We find that neither of the algorithms studied is intrinsically superior to the other; rather, the ideal algorithm as well as the ideal algorithm parameters depend on the purpose for which groups are to be studied. The Huchra/Geller algorithm misses few real groups, at the cost of including some spurious groups and members, while the Nolthenius/White algorithm misses high velocity dispersion groups and members but is less likely to include interlopers in its group assignments. In a companion paper we investigate the accuracy of virial mass estimates and clustering properties of groups identified using these algorithms.

  16. Core Transcriptional Regulatory Circuit Controlled by the TAL1 Complex in Human T Cell Acute Lymphoblastic Leukemia

    E-Print Network [OSTI]

    Sanda, Takaomi

    The oncogenic transcription factor TAL1/SCL is aberrantly expressed in over 40% of cases of human T cell acute lymphoblastic leukemia (T-ALL), emphasizing its importance in the molecular pathogenesis of T-ALL. Here we ...

  17. Genome-Wide Profiling of H3K56 Acetylation and Transcription Factor Binding Sites in Human Adipocytes

    E-Print Network [OSTI]

    Lo, Kin Yui

    The growing epidemic of obesity and metabolic diseases calls for a better understanding of adipocyte biology. The regulation of transcription in adipocytes is particularly important, as it is a target for several therapeutic ...

  18. A genome-wide regulatory network identifies key transcription factors for memory CD8[superscript +] T-cell development

    E-Print Network [OSTI]

    Hu, Guangan

    Memory CD8[superscript +] T-cell development is defined by the expression of a specific set of memory signature genes. Despite recent progress, many components of the transcriptional control of memory CD8[superscript +] ...

  19. Linking high-resolution metabolic flux phenotypes and transcriptional regulation in yeast modulated by the global regulator Gcn4p

    E-Print Network [OSTI]

    Moxley, Joel F.

    Genome sequencing dramatically increased our ability to understand cellular response to perturbation. Integrating system-wide measurements such as gene expression with networks of protein–protein interactions and transcription ...

  20. SurR: A transcriptional activator and repressor controlling hydrogen and elemental sulfur metabolism in Pyrococcus furiosus

    E-Print Network [OSTI]

    Scott, Robert A.

    metabolism in Pyrococcus furiosus Lipscomb, Gina L.,1 Annette M. Keese,3 Darin M. Cowart,2 Gerrit J. Schut,1, 2005). TFB and RNA polymerase together define the transcription start site position, 25 to 30 bp

  1. Curated collection of yeast transcription factor DNA binding specificity data reveals novel structural and gene regulatory insights

    E-Print Network [OSTI]

    Gordan, Raluca

    Background: Transcription factors (TFs) play a central role in regulating gene expression by interacting with cis-regulatory DNA elements associated with their target genes. Recent surveys have examined the DNA binding ...

  2. Identification of Tat-SF1 cellular targets by exon array analysis reveals dual roles in transcription and splicing

    E-Print Network [OSTI]

    Hartemink, Alexander

    acid metabolism; the insulin signaling pathway was enriched with Tat-SF1 transcript-level targets discovered over a decade ago as a cellular protein required for Tat-specific, TAR-dependent activation of HIV

  3. Last Updated: October 5, 2011 1 iSiS -Generating an Unofficial Transcript via Faculty Center

    E-Print Network [OSTI]

    Massachusetts at Lowell, University of

    Last Updated: October 5, 2011 1 iSiS - Generating an Unofficial Transcript via Faculty Center 1 browser as a trusted site. For instructions: http://www.uml.edu/enrollment/isis

  4. Cartilage mechanobiology and transcriptional effects of combined mechanical compression and IGF-1 stimulation on bovine cartilage explants

    E-Print Network [OSTI]

    Wheeler, Cameron, 1978-

    2007-01-01T23:59:59.000Z

    Background: Investigators have focused on mechano-regulation of upstream signaling and responses at the level of gene transcription, protein translation and post-translational modifications. Intracellular pathways including ...

  5. The construction and use of a plasmid vector system for studying Q mediated transcription termination in bacteriophage (lambda)

    E-Print Network [OSTI]

    Eller, Richard Frank

    1986-01-01T23:59:59.000Z

    THE CONSTRUCTION AND USE OF A PLASMID VECTOR SYSTEM FOR STUDYING Q MEDIATED TRANSCRIPTION TERMINATION IN BACTERIOPHAGE l A THESIS BY RICHARD FRANK ELLER Submitted to the Graduate College of TEXAS A&M UNIVERSITY in partial fulfillment... of the requirements for the degree of MASTER OF SCIENCE May 19&6 Major Subject: Biochemistry THE CONSTRUCTION AND USE OF A PLASMID VECTOR SYSTEM FOR STUDYING Q MEDIATED TRANSCRIPTION TERMINATION IN BACTERIOPHAGE A THESIS BY RICHARD FRANK ELLER Approved...

  6. The oncoprotein HBXIP upregulates PDGFB via activating transcription factor Sp1 to promote the proliferation of breast cancer cells

    SciTech Connect (OSTI)

    Zhang, Yingyi; Zhao, Yu; Li, Leilei; Shen, Yu; Cai, Xiaoli [Department of Biochemistry, College of Life Sciences, Nankai University, Tianjin 300071 (China)] [Department of Biochemistry, College of Life Sciences, Nankai University, Tianjin 300071 (China); Zhang, Xiaodong, E-mail: zhangxd@nankai.edu.cn [Department of Cancer Research, Institute for Molecular Biology, College of Life Sciences, Nankai University, Tianjin 300071 (China)] [Department of Cancer Research, Institute for Molecular Biology, College of Life Sciences, Nankai University, Tianjin 300071 (China); Ye, Lihong, E-mail: yelihong@nankai.edu.cn [Department of Biochemistry, College of Life Sciences, Nankai University, Tianjin 300071 (China)] [Department of Biochemistry, College of Life Sciences, Nankai University, Tianjin 300071 (China)

    2013-05-03T23:59:59.000Z

    Highlights: •HBXIP is able to upregulate the expression of PDGFB in breast cancer cells. •HBXIP serves as a coactivator of activating transcription factor Sp1. •HBXIP stimulates the PDGFB promoter via activating transcription factor Sp1. •HBXIP promotes the proliferation of breast cancer cell via upregulating PDGFB. -- Abstract: We have reported that the oncoprotein hepatitis B virus X-interacting protein (HBXIP) acts as a novel transcriptional coactivator to promote proliferation and migration of breast cancer cells. Previously, we showed that HBXIP was able to activate nuclear factor-?B (NF-?B) in breast cancer cells. As an oncogene, the platelet-derived growth factor beta polypeptide (PDGFB) plays crucial roles in carcinogenesis. In the present study, we found that both HBXIP and PDGFB were highly expressed in breast cancer cell lines. Interestingly, HBXIP was able to increase transcriptional activity of NF-?B through PDGFB, suggesting that HBXIP is associated with PDGFB in the cells. Moreover, HBXIP was able to upregulate PDGFB at the levels of mRNA, protein and promoter in the cells. Then, we identified that HBXIP stimulated the promoter of PDGFB through activating transcription factor Sp1. In function, HBXIP enhanced the proliferation of breast cancer cells through PDGFB in vitro. Thus, we conclude that HBXIP upregulates PDGFB via activating transcription factor Sp1 to promote proliferation of breast cancer cells.

  7. Unusual thoracic radiographic findings in children treated for Hodgkin's disease

    SciTech Connect (OSTI)

    Jochelson, M.S.; Tarbell, N.J.; Weinstein, H.J.

    1986-06-01T23:59:59.000Z

    Mantle irradiation is often part of the treatment for Hodgkin's disease. Localized pneumonitis and fibrosis are well-known sequelae of this treatment. We report nine patients with unusual thoracic radiographic findings following treatment for Hodgkin's disease. All nine had mediastinal widening. Seven of these patients received combined modality therapy in which prednisone was given with their MOPP. In these seven patients, an increase in mediastinal width developed at the same time as the radiographic changes of radiation pneumonitis. Two patients developed bilateral infiltrates extending beyond the field of radiation to the lung periphery. In one of these patients, a spontaneous pneumomediastinum developed. One patient underwent mediastinal biopsy that revealed inflammatory changes similar to those seen in radiation pneumonitis. All patients either responded to steroids or had spontaneous regression of radiographic abnormalities supporting the presumed diagnosis of treatment related changes. Recognition of these unusual sequelae of mantle irradiation will aid in differentiating them from infection or tumor and lead to prompt, appropriate treatment.

  8. Up-and-Down and the Percentile-Finding Problem

    E-Print Network [OSTI]

    Oron, Assaf P

    2008-01-01T23:59:59.000Z

    Up-and-Down (U&D) is a popular sequential design for estimating threshold percentiles in binary experiments. However, U&D application practices have stagnated, and significant gaps in understanding its properties persist. The first part of my work aims to fill gaps in U&D theory. New results concerning stationary distribution properties are proven. A second focus of this study is nonparametric U&D estimation. An improvement to isotonic regression called "centered isotonic regression" (CIR), and a new averaging estimator called "auto-detect" are introduced and their properties studied. Bayesian percentile-finding designs, most notably the continual reassessment method (CRM) developed for Phase I clinical trials, are also studied. In general, CRM convergence depends upon random run-time conditions -- meaning that convergence is not always assured. Small-sample behavior is studied as well. It is shown that CRM is quite sensitive to outlier sub-sequences of thresholds, resulting in highly variable...

  9. Transcript & Video

    E-Print Network [OSTI]

    Hull, Kathleen

    2009-01-01T23:59:59.000Z

    and Resource Allocation, for synthesis and assessment, aspractices of resource flows and faculty assessment, and they

  10. Transcription Factor Amr1 Induces Melanin Biosynthesis and Suppresses Virulence in Alternaria brassicicola

    SciTech Connect (OSTI)

    Cho, Yangrae; Srivastava, Akhil; Ohm, Robin A.; Lawrence, Christopher B.; Wang, Koon-Hui; Grigoriev, Igor V.; Marahatta, Sharadchandra P.

    2012-05-01T23:59:59.000Z

    Alternaria brassicicola is a successful saprophyte and necrotrophic plant pathogen. Several A. brassicicola genes have been characterized as affecting pathogenesis of Brassica species. To study regulatory mechanisms of pathogenesis, we mined 421 genes in silico encoding putative transcription factors in a machine-annotated, draft genome sequence of A. brassicicola. In this study, targeted gene disruption mutants for 117 of the transcription factor genes were produced and screened. Three of these genes were associated with pathogenesis. Disruption mutants of one gene (AbPacC) were nonpathogenic and another gene (AbVf8) caused lesions less than half the diameter of wild-type lesions. Unexpectedly, mutants of the third gene, Amr1, caused lesions with a two-fold larger diameter than the wild type and complementation mutants. Amr1 is a homolog of Cmr1, a transcription factor that regulates melanin biosynthesis in several fungi. We created gene deletion mutants of ?amr1 and characterized their phenotypes. The ?amr1 mutants used pectin as a carbon source more efficiently than the wild type, were melanin-deficient, and more sensitive to UV light and glucanase digestion. The AMR1 protein was localized in the nuclei of hyphae and in highly melanized conidia during the late stage of plant pathogenesis. RNA-seq analysis revealed that three genes in the melanin biosynthesis pathway, along with the deleted Amr1 gene, were expressed at low levels in the mutants. In contrast, many hydrolytic enzyme-coding genes were expressed at higher levels in the mutants than in the wild type during pathogenesis. The results of this study suggested that a gene important for survival in nature negatively affected virulence, probably by a less efficient use of plant cell-wall materials. We speculate that the functions of the Amr1 gene are important to the success of A. brassicicola as a competitive saprophyte and plant parasite.

  11. ECRbase: Database of Evolutionary Conserved Regions, Promoters, and Transcription Factor Binding Sites in Vertebrate Genomes

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Loots, Gabriela G. [LLNL; Ovcharenko, I. [LLNL

    Evolutionary conservation of DNA sequences provides a tool for the identification of functional elements in genomes. This database of evolutionary conserved regions (ECRs) in vertebrate genomes features a database of syntenic blocks that recapitulate the evolution of rearrangements in vertebrates and a comprehensive collection of promoters in all vertebrate genomes generated using multiple sources of gene annotation. The database also contains a collection of annotated transcription factor binding sites (TFBSs) in evolutionary conserved and promoter elements. ECRbase currently includes human, rhesus macaque, dog, opossum, rat, mouse, chicken, frog, zebrafish, and fugu genomes. (taken from paper in Journal: Bioinformatics, November 7, 2006, pp. 122-124

  12. Transcript for video: http://www.youtube.com/watch?v=m-Qvf

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of ScienceandMesa del SolStrengthening a solidSynthesisAppliances » TopToursTrainingTranscript for

  13. Growing Up in Scotland: Sweep 2 - Summary of Findings from Year 2 

    E-Print Network [OSTI]

    Government, Scottish

    2008-02-01T23:59:59.000Z

    Research findings (of four) accompanying the full research report on Sweep 2 findings of the Growing Up in Scotland Study Year 2...

  14. mRNA Transcript Abundance during Plant Growth and the Influence of Li+ Exposure

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Duff, M. C.; Kuhne, W. W.; Halverson, N.; Chang, C. -S.; Kitamura, E.; Hawthorn, L.; Milliken, C. E.; Caldwell, E. F.; Stieve-Caldwell, E.; Martinez, N. E.; et al

    2014-12-01T23:59:59.000Z

    Lithium (Li) toxicity in plants is, at a minimum, a function of Li+ concentration, exposure time, species and growth conditions. Most plant studies with Li+ focus on short-term acute exposures. This study examines short- and long-term effects of Li+ exposure in Arabidopsis with Li+ uptake studies and measured shoot mRNA transcript abundance levels in treated and control plants. Stress, pathogen-response and arabinogalactan protein genes were typically more up-regulated in older (chronic, low level) Li+-treatment plants and in the much younger plants from acute high-level exposures. The gene regulation behavior of high-level Li+ resembled prior studies due to its influence on:more »inositol synthesis, 1-aminocyclopropane-1-carboxylate synthases and membrane ion transport. In contrast, chronically-exposed plants had gene regulation responses that were indicative of pathogen, cold, and heavy-metal stress, cell wall degradation, ethylene production, signal transduction, and calcium-release modulation. Acute Li+ exposure phenocopies magnesium-deficiency symptoms and is associated with elevated expression of stress response genes that could lead to consumption of metabolic and transcriptional energy reserves and the dedication of more resources to cell development. In contrast, chronic Li+ exposure increases expression signal transduction genes. The identification of new Li+-sensitive genes and a gene-based “response plan” for acute and chronic Li+ exposure are delineated.« less

  15. mRNA Transcript Abundance during Plant Growth and the Influence of Li+ Exposure

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Duff, M. C. [Savannah River National Laboratory, Aiken, SC (United States); Kuhne, W. W. [Savannah River National Laboratory, Aiken, SC (United States); Halverson, N. [Savannah River National Laboratory, Aiken, SC (United States); Chang, C. -S. [Georgia Regents University Cancer Center, Augusta, GA (United States). Integrated Genomics Core; Kitamura, E. [Georgia Regents University Cancer Center, Augusta, GA (United States). Integrated Genomics Core; Hawthorn, L. [Georgia Regents University Cancer Center, Augusta, GA (United States). Integrated Genomics Core; Milliken, C. E. [Savannah River National Laboratory, Aiken, SC (United States); Caldwell, E. F. [Savannah River National Laboratory, Aiken, SC (United States); Stieve-Caldwell, E. [Savannah River National Laboratory, Aiken, SC (United States); Martinez, N. E. [Savannah River National Laboratory, Aiken, SC (United States); Colorado State University, Ft. Collins, CO (United States). Dept. of Environmental and Radiological Health Sciences; Stafford, C. [Savannah River National Laboratory, Aiken, SC (United States); Univ. of South Carolina Medical School, Columbia, SC (United States)

    2014-12-01T23:59:59.000Z

    Lithium (Li) toxicity in plants is, at a minimum, a function of Li+ concentration, exposure time, species and growth conditions. Most plant studies with Li+ focus on short-term acute exposures. This study examines short- and long-term effects of Li+ exposure in Arabidopsis with Li+ uptake studies and measured shoot mRNA transcript abundance levels in treated and control plants. Stress, pathogen-response and arabinogalactan protein genes were typically more up-regulated in older (chronic, low level) Li+-treatment plants and in the much younger plants from acute high-level exposures. The gene regulation behavior of high-level Li+ resembled prior studies due to its influence on: inositol synthesis, 1-aminocyclopropane-1-carboxylate synthases and membrane ion transport. In contrast, chronically-exposed plants had gene regulation responses that were indicative of pathogen, cold, and heavy-metal stress, cell wall degradation, ethylene production, signal transduction, and calcium-release modulation. Acute Li+ exposure phenocopies magnesium-deficiency symptoms and is associated with elevated expression of stress response genes that could lead to consumption of metabolic and transcriptional energy reserves and the dedication of more resources to cell development. In contrast, chronic Li+ exposure increases expression signal transduction genes. The identification of new Li+-sensitive genes and a gene-based “response plan” for acute and chronic Li+ exposure are delineated.

  16. Transcript levels and synthesis of photosystem II components in cyanobacterial mutants with inactivated photosystem II genes

    SciTech Connect (OSTI)

    Jiujiang Yu; Vermaas, W.F.J. (Arizona State Univ., Tempe (United States))

    1990-04-01T23:59:59.000Z

    After interruption or deletion of the photosystem II genes psbB, psbC, and psbD in the cyanobacterium Synechocystis sp. PCC 6803, thylakoids from such mutants were found to be depleted in a number of photosystem II proteins in addition to those for which the gene(s) had been inactivated. Transcript levels of photosystem II genes were measured and protein pulse-labeling was carried out to determine the reason for this effect. Transcripts of all photosystem II genes except the inactivated one(s) were found to be present in the various mutants. In certain cases, inactivation of one photosystem II gene led to overexpression of another. Protein pulse-labeling experiments using {sup 35}S-methionine, in which not only the rapidly turing over D1 protein but also D2, CP43, and CP47 appear to be preferentially labeled, showed that the mutants studied synthesize the D1 protein as well as other photosystem II proteins whose genes were not inactivated. The fact that, in the various mutants, photosystem II proteins for which the gene is not inactivated are synthesized but do not accumulate in the thylakoid indicates that the psbB, psbC, and psbD gene products are all required for a stable assembly of the photosystem II complex.

  17. FINDING SOLUTIONS AT THE WEST VALLEY DEMONSTRATION PROJECT

    SciTech Connect (OSTI)

    Drake, John L.; Gramling, James M.; Houston, Helene M.

    2003-02-27T23:59:59.000Z

    The United States Department of Energy Office of Environmental Management (DOE-EM) faces a number of sizeable challenges as it begins to transform its mission from managing risk to reducing and eliminating risk throughout the DOE Complex. One of the greatest challenges being addressed by DOE-EM as this transformation takes place is accelerating the deactivation and decommissioning of thousands of facilities within the DOE Complex that were once used to support nuclear-related programs and projects. These facilities are now unused and aging. Finding solutions to complete the cleanup of these aging facilities more safely, efficiently, and effectively while reducing costs is critical to successfully meeting DOE-EM's cleanup challenge. The Large-Scale Demonstration and Deployment Project (LSDDP) of Hot Cells at the West Valley Demonstration Project (WVDP) is a near-term project funded through the DOE's National Energy Technology Laboratory (DOE-NETL) for the specific purpose of identifying, evaluating, demonstrating, and deploying commercially available technologies that are capable of streamlining the cleanup of hot cells in unused facilities while improving worker safety. Two DOE project sites are participating in this LSDDP: the WVDP site in West Valley, New York and the Hanford River Corridor Project (RCP) site in Richland, Washington. The WVDP site serves as the host site for the project. Technologies considered for demonstration and potential deployment at both LSDDP sites are targeted for application in hot cells that require the use of remote and semi-remote techniques to conduct various cleanup-related activities because of high radiation or high contamination levels. These hot cells, the type of cleanup activities being conducted, and technologies selected for demonstration are the main topics discussed in this paper. The range of cleanup-related activities addressed include in-situ characterization, size-reduction, contamination control, decontamination, in-c ell viewing, and various types of handling, retrieval, and dismantlement tasks. The primary focus of the LSDDP of Hot Cells is on demonstrating technologies capable of reducing cost and schedule baselines for work scopes involving in-situ characterization (including nondestructive examination to access in-cell areas), size-reducing equipment and piping, contamination control, and decontaminating surfaces (including equipment surfaces). Demonstrations of technologies that can streamline these tasks are scheduled for the WVDP site. Demonstrations scheduled for the Hanford RCP site focus on work scope activities involving remote-inspection and viewing. Each demonstration conducted will be assessed using evaluation criteria established by the participating sites to determine if selected technologies represent a significant improvement over current baseline technologies being used to perform work. If proven to be effective, each of the commercially available technologies demonstrated has th e potential to be quickly deployed at other sites, resulting in improved worker safety, reduced cleanup costs, and accelerated schedule completion for many of the most challenging cleanup efforts now underway throughout the DOE Complex.

  18. On-chip single-copy real-time reverse-transcription PCR in isolated picoliter droplets

    SciTech Connect (OSTI)

    Beer, N R; Wheeler, E; Lee-Houghton, L; Watkins, N; Nasarabadi, S; Hebert, N; Leung, P; Arnold, D; Bailey, C; Colston, B

    2007-12-19T23:59:59.000Z

    The first lab-on-chip system for picoliter droplet generation and RNA isolation, followed by reverse transcription, and PCR amplification with real-time fluorescence detection in the trapped droplets has been developed. The system utilized a shearing T-junction in a fused silica device to generate a stream of monodisperse picoliter-scale droplets that were isolated from the microfluidic channel walls and each other by the oil phase carrier. An off-chip valving system stopped the droplets on-chip, allowing thermal cycling for reverse transcription and subsequent PCR amplification without droplet motion. This combination of the established real-time reverse transcription-PCR assay with digital microfluidics is ideal for isolating single-copy RNA and virions from a complex environment, and will be useful in viral discovery and gene-profiling applications.

  19. Prevalence of Incidental Findings on Abdominal Computed Tomography Angiograms on Prospective Renal Donors

    E-Print Network [OSTI]

    2015-01-01T23:59:59.000Z

    multidetector computed tomography angiography. Transplantof Radiology CT: computed tomography IF: incidental findings

  20. Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus

    DOE Patents [OSTI]

    Dai, Ziyu; Lasure, Linda L; Magnuson, Jon K

    2014-05-27T23:59:59.000Z

    The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus.

  1. Oestradiol reduces Liver Receptor Homolog-1 mRNA transcript stability in breast cancer cell lines

    SciTech Connect (OSTI)

    Lazarus, Kyren A. [Cancer Drug Discovery Laboratory, Prince Henry’s Institute of Medical Research, Clayton, Victoria 3168 (Australia) [Cancer Drug Discovery Laboratory, Prince Henry’s Institute of Medical Research, Clayton, Victoria 3168 (Australia); Environmental and Biotechnology Centre, Swinburne University, Hawthorn, Victoria 3122 (Australia); Zhao, Zhe; Knower, Kevin C. [Cancer Drug Discovery Laboratory, Prince Henry’s Institute of Medical Research, Clayton, Victoria 3168 (Australia)] [Cancer Drug Discovery Laboratory, Prince Henry’s Institute of Medical Research, Clayton, Victoria 3168 (Australia); To, Sarah Q. [Cancer Drug Discovery Laboratory, Prince Henry’s Institute of Medical Research, Clayton, Victoria 3168 (Australia) [Cancer Drug Discovery Laboratory, Prince Henry’s Institute of Medical Research, Clayton, Victoria 3168 (Australia); Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3168 (Australia); Chand, Ashwini L. [Cancer Drug Discovery Laboratory, Prince Henry’s Institute of Medical Research, Clayton, Victoria 3168 (Australia)] [Cancer Drug Discovery Laboratory, Prince Henry’s Institute of Medical Research, Clayton, Victoria 3168 (Australia); Clyne, Colin D., E-mail: Colin.clyne@princehenrys.org [Cancer Drug Discovery Laboratory, Prince Henry’s Institute of Medical Research, Clayton, Victoria 3168 (Australia); Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3168 (Australia)

    2013-08-30T23:59:59.000Z

    Highlights: •LRH-1 is an orphan nuclear receptor that regulates tumor proliferation. •In breast cancer, high mRNA expression is associated with ER+ status. •In ER?ve cells, despite very low mRNA, we found abundant LRH-1 protein. •Our data show distinctly different LRH-1 protein isoforms in ER? and ER+ breast cancer cells. •This is due to differences in LRH-1 mRNA and protein stability rates. -- Abstract: The expression of orphan nuclear receptor Liver Receptor Homolog-1 (LRH-1) is elevated in breast cancer and promotes proliferation, migration and invasion in vitro. LRH-1 expression is regulated by oestrogen (E{sub 2}), with LRH-1 mRNA transcript levels higher in oestrogen receptor ? (ER?) positive (ER+) breast cancer cells compared to ER? cells. However, the presence of LRH-1 protein in ER? cells suggests discordance between mRNA transcript levels and protein expression. To understand this, we investigated the impact of mRNA and protein stability in determining LRH-1 protein levels in breast cancer cells. LRH-1 transcript levels were significantly higher in ER+ versus ER? breast cancer cells lines; however LRH-1 protein was expressed at similar levels. We found LRH-1 mRNA and protein was more stable in ER? compared to ER+ cell lines. The tumor-specific LRH-1 variant isoform, LRH-1v4, which is highly responsive to E{sub 2}, showed increased mRNA stability in ER? versus ER+ cells. In addition, in MCF-7 and T47-D cell lines, LRH-1 total mRNA stability was reduced with E{sub 2} treatment, this effect mediated by ER?. Our data demonstrates that in ER? cells, increased mRNA and protein stability contribute to the abundant protein expression levels. Expression and immunolocalisation of LRH-1 in ER? cells as well as ER? tumors suggests a possible role in the development of ER? tumors. The modulation of LRH-1 bioactivity may therefore be beneficial as a treatment option in both ER? and ER+ breast cancer.

  2. Involvement of the transcription factor FoxM1 in contact inhibition

    SciTech Connect (OSTI)

    Faust, Dagmar; Al-Butmeh, Firas; Linz, Berenike [Institute of Toxicology, Medical Center of the Johannes Gutenberg-University, Obere Zahlbacherstr. 67, 55131 Mainz (Germany)] [Institute of Toxicology, Medical Center of the Johannes Gutenberg-University, Obere Zahlbacherstr. 67, 55131 Mainz (Germany); Dietrich, Cornelia, E-mail: cdietric@uni-mainz.de [Institute of Toxicology, Medical Center of the Johannes Gutenberg-University, Obere Zahlbacherstr. 67, 55131 Mainz (Germany)] [Institute of Toxicology, Medical Center of the Johannes Gutenberg-University, Obere Zahlbacherstr. 67, 55131 Mainz (Germany)

    2012-10-05T23:59:59.000Z

    Highlights: Black-Right-Pointing-Pointer The transcription factor FoxM1 is downregulated upon contact inhibition. Black-Right-Pointing-Pointer The decrease in FoxM1 levels occurs very likely due to inhibition of ERK activity. Black-Right-Pointing-Pointer The decrease in FoxM1 is not sufficient, but required for contact inhibition. Black-Right-Pointing-Pointer We propose a new model of contact inhibition involving pRB/E2F and FoxM1. -- Abstract: Contact inhibition is a crucial mechanism regulating proliferation in vitro and in vivo. Although it is generally accepted that contact inhibition plays a pivotal role in maintaining tissue homeostasis, the molecular mechanisms of contact inhibition are still not fully understood. FoxM1 is known as a proliferation-associated transcription factor and is upregulated in many cancer types. Vice versa, anti-proliferative signals, such as TGF-{beta} and differentiation signals decrease FoxM1 expression. Here we investigated the role of FoxM1 in contact inhibition in fibroblasts. We show that protein expression of FoxM1 is severely and rapidly downregulated upon contact inhibition, probably by inhibition of ERK activity, which then leads to decreased expression of cyclin A and polo-like kinase 1. Vice versa, ectopic expression of FoxM1 prevents the decrease in cyclin A and polo-like kinase 1 and causes a two-fold increase in saturation density indicating loss of contact inhibition. Hence, we show that downregulation of FoxM1 is required for contact inhibition by regulating expression of cyclin A and polo-like kinase 1.

  3. A Quality Control Pathway That Down-regulates Aberrant T-cell Receptor (TCR) Transcripts by a Mechanism Requiring UPF2 and

    E-Print Network [OSTI]

    Wilkinson, Miles F.

    by a Mechanism Requiring UPF2 and Translation* Received for publication, December 10, 2001, and in revised form as antisense hUPF2 transcripts increased the levels of PTC-bearing TCR- transcripts in the nuclear fraction of cells. We conclude that a hUPF2-dependent RNA sur- veillance pathway with translation-like features

  4. Queen of the Sun Transcript Welcome to Bug Bytes, a bimonthly podcast from Texas A&M University's

    E-Print Network [OSTI]

    Behmer, Spencer T.

    Bug Bytes Queen of the Sun Transcript **Music Up Welcome to Bug Bytes, a bimonthly podcast from beekeepers of the world. Why? We recently screened a documentary called Queen of the Sun. The film explores in the face of almost certain impending disaster. We were prepared for this, as we thought Queen of the Sun

  5. Architecture and anatomy of the genomic locus encoding the human leukemia-associated transcription factor RUNX1/AML1q

    E-Print Network [OSTI]

    Groner, Yoram

    involved in acute myeloid leukemia. Interestingly, the FLI1 homologous sequence contains a breakpoint.elsevier.com/locate/gene Abbreviations: All, acute lymphoid leukemia; AML, acute myeloid leukemia; CBFb, core binding factor b; DS, DownArchitecture and anatomy of the genomic locus encoding the human leukemia-associated transcription

  6. Herpes Simplex Virus 1 ICP27 Is Required for Transcription of Two Viral Late ( 2) Genes in Infected Cells

    E-Print Network [OSTI]

    Knipe, David M.

    Herpes Simplex Virus 1 ICP27 Is Required for Transcription of Two Viral Late ( 2) Genes in Infected January 18, 2001; accepted March 1, 2001 The herpes simplex virus infected cell protein 27 (ICP27 expression during productive infection by herpes simplex virus 1 is well doc- umented (Clements et al., 1977

  7. Cell, Vol. 73, 1197-1206, June 16, 1993, Copyright 0 1993 by Cell Press Transcriptional Induction of Genes Encoding

    E-Print Network [OSTI]

    Walter, Peter

    to assist proper folding and to prevent aggregation of folding intermediates (Pelham, 1986; Haas and Wabl according to demand for them inside the or- ganelle. In mammalian cells, for example, synthesis of BiP, PDI occurs at the transcriptional level (Lee, 1987). Thus, an unfolded protein response pathway must exist

  8. Inhibition of Heat Shock Transcription Factor SUBHAGYA A. WADEKAR , DAPEI LI, SUMUDRA PERIYASAMY, AND EDWIN R. SA NCHEZ

    E-Print Network [OSTI]

    Abraham, Nader G.

    to stress, speculation has ex- isted that communication between the heat shock and glucocorticoid hormoneInhibition of Heat Shock Transcription Factor by GR SUBHAGYA A. WADEKAR , DAPEI LI, SUMUDRA mammalian heat shock proteins. As heat shock proteins are produced by cells as an adaptive response

  9. Application Form for an Apostille/State Seal on Transcripts Complete this form and send it along with

    E-Print Network [OSTI]

    Plotkin, Joshua B.

    & delivery fees to Harrisburg as well as Express return delivery to a US address). $90.00 (includes transcript, notary & delivery fees to Harrisburg as well as Express return delivery to an International be included to cover the costs assessed in Harrisburg. #12;

  10. Transcript Notation Information for Faculty For the first time beginning with the June, 2012 graduation, OSU students

    E-Print Network [OSTI]

    Escher, Christine

    graduation, OSU students who have engaged in significant research/creative activities that meet the criteria such a transcript notation include completing a project with the following elements: § Involvement of the student in all major phases of their project including conception, implementation, and presentation

  11. E2F1 represses b-catenin transcription and is antagonized by both pRB and CDK8

    E-Print Network [OSTI]

    Cai, Long

    LETTERS E2F1 represses b-catenin transcription and is antagonized by both pRB and CDK8 Erick suppressor protein (pRB). Here we show that E2F1 is a potent and specific inhibitor of b

  12. Estrogen-Related Receptor A1 Transcriptional Activities Are Regulated in Part via the ErbB2/HER2

    E-Print Network [OSTI]

    Mertz, Janet E.

    MCF-7 mammary carcinoma cells, and (b) ERRA and ErbB2 mRNA levels positively correlate in clinical breast tumors. We show here that ERRA1 represses ERA-mediated activation in MCF-7 cells because it failed plasmids. Importantly, overexpression of activated ErbB2 in MCF-7 cells led to transcriptional activation

  13. Accepted Article Preview: Published ahead of advance online publication Transcription Factor/microRNA Axis Blocks Melanoma

    E-Print Network [OSTI]

    Shamir, Ron

    /microRNA Axis Blocks Melanoma Invasion Program by miR-211 Targeting NUAK1 Rachel E Bell, Mehdi Khaled, Dvir Levy, Transcription Factor/microRNA Axis Blocks Melanoma Invasion Program by miR-211 Targeting NUAK1 Blocks Melanoma Invasion Program by miR-211 Targeting NUAK1 Rachel E. Bella,h , Mehdi Khaledb

  14. Cell, Vol. 106, 697--708, September 21, 2001, Copyright #2001 by Cell Press Serial Regulation of Transcriptional Regulators

    E-Print Network [OSTI]

    Gifford, David K.

    Cambridge, Massachusetts 02142 extrapolate to regulation of all cell cycle genes. Microarray analysis hasCell, Vol. 106, 697--708, September 21, 2001, Copyright #2001 by Cell Press Serial Regulation of Transcriptional Regulators in the Yeast Cell Cycle (a complex of Swi4 and Swi6) control late G1 genes. Mcm1

  15. Retirement Transcript Slide1. Welcome to Florida State University, this section is an overview of the Retirement

    E-Print Network [OSTI]

    Sura, Philip

    date. As long as you are vested under the plan, you may choose to retire early, before reachingRetirement Transcript Slide1. Welcome to Florida State University, this section is an overview of the Retirement options available to all salaried USPS, A& P, and faculty employees. We are pleased to offer

  16. The Energy Sensor AMP-activated Protein Kinase Directly Regulates the Mammalian FOXO3 Transcription Factor*S

    E-Print Network [OSTI]

    Brunet, Anne

    The Energy Sensor AMP-activated Protein Kinase Directly Regulates the Mammalian FOXO3 Transcription of homeostasis throughout an organism's life span requires constant adaptation to changes in energy lev- els. The AMP-activated protein kinase (AMPK) plays a critical role in the cellular responses to low energy

  17. Homotypic clusters of transcription factor binding sites: a model system for understanding the physical mechanics of gene expression

    E-Print Network [OSTI]

    Ezer, Daphne; Zabet, Nicolae Radu; Adryan, Boris

    2014-08-01T23:59:59.000Z

    hundred base pairs immediately adjacent to the transcription start ess o ind ane pres eby rier e e67D. Ezer et al. / Computational and Structural Biotechnology Journal 10 (2014) 63–69site. Our previous work cataloged all promoter organizations in E. coli K...

  18. Finding new reserves of oil and gas As the world's reserves of oil and gas become exhausted, we urgently need to find new

    E-Print Network [OSTI]

    Anderson, Jim

    Finding new reserves of oil and gas As the world's reserves of oil and gas become exhausted, we urgently need to find new fields to answer our energy needs. Oil companies are keen to use novel techniques) techniques represent arguably the most significant technological advance in the field of oil exploration

  19. Finding & Citing Journal Articles Sept 2008 There are many journal indexes that can help you find citations to journal articles, and in

    E-Print Network [OSTI]

    Abolmaesumi, Purang

    Finding & Citing Journal Articles Sept 2008 There are many journal indexes that can help you find citations to journal articles, and in many cases, full-text electronic versions of these articles. For your convenience, journal indexes have been grouped by subject. We'll use `geography' in this example. A. Success

  20. EI2 Insulation Helps Anxious Pooch Find Calm in the Storm | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    EI2 Insulation Helps Anxious Pooch Find Calm in the Storm EI2 Insulation Helps Anxious Pooch Find Calm in the Storm Photo of a dog sitting on a bed or couch with a stuffed toy in...

  1. Findings and Recommendations by the California Carbon Capture and Storage Review Panel

    E-Print Network [OSTI]

    Sequestration Partnership #12;Findings and Recommendations by the California Carbon Capture and Storage ReviewFindings and Recommendations by the California Carbon Capture and Storage Review Panel December............................................................................1 Creation of the Carbon Capture and Storage Review Panel

  2. A Cone Jet-Finding Algorithm for Heavy-Ion Collisions at LHC Energies

    E-Print Network [OSTI]

    2008-01-01T23:59:59.000Z

    G 30 (2004) S1155 A Cone Jet-Finding Algorithm for Heavy-IonA Cone Jet-Finding Algorithm for Heavy-Ion Collisions at LHCSwitzerland Abstract. Standard jet ?nding techniques used in

  3. Report on Hydrogen Storage Panel Findings in DOE-BES Sponsored...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Hydrogen Storage Panel Findings in DOE-BES Sponsored Workshop on Basic Research for Hydrogen Production, Storage and Use Report on Hydrogen Storage Panel Findings in DOE-BES...

  4. Scientists at ALS Find New Path to More Efficient Organic Solar...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Scientists at ALS Find New Path to More Efficient Organic Solar Cells Scientists at ALS Find New Path to More Efficient Organic Solar Cells Print Monday, 07 January 2013 00:00...

  5. Not-so-incidental findings: the ACMG recommendations on the reporting of incidental

    E-Print Network [OSTI]

    enter the clinical realm, these concerns are extended to individually relevant findings acknowledging that the term `incidental findings' has indicated `unexpected positive findings' in the past to indicate the results of a deliberate search for pathogenic or likely pathogenic alterations in genes

  6. HSS Finding D3 Corrective Action Plan for LBNL Integrated ES&H Management Inspection

    E-Print Network [OSTI]

    Knowles, David William

    LNBL CAP HSS Finding D3 Corrective Action Plan for LBNL Integrated ES&H Management Inspection Owner: Ross Fisher Analyst: Weyland Wong Finding Statement D3: LBNL has not established sufficient processes for Correcting Finding: Richard DeBusk LBNL EH&S Division Laboratory Safety Manager (510) 495-2976 REDe

  7. Finding Jung

    E-Print Network [OSTI]

    McMillan, Frank N.

    2012-03-29T23:59:59.000Z

    spirit, and a theme which has to prove of such relevance that Dr. Anthony Stevens was able to express it in a more contemporary and immediate sense in the lectures (and book that followed5) he gave in the series of lectures Carolyn Fay has endowed...

  8. Find Books

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of Science (SC) Environmental Assessments (EA)Budget »Travel andFifthDepartment of

  9. Find Journals

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of Science (SC) Environmental Assessments (EA)Budget »Travel andFifthDepartment

  10. Find Standards

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of Science (SC) Environmental Assessments (EA)Budget »Travel andFifthDepartmentPatentsReports

  11. Global transcriptional, physiological and metabolite analyses of Desulfovibrio vulgaris Hildenborough responses to salt adaptation

    SciTech Connect (OSTI)

    He, Z.; Zhou, A.; Baidoo, E.; He, Q.; Joachimiak, M. P.; Benke, P.; Phan, R.; Mukhopadhyay, A.; Hemme, C.L.; Huang, K.; Alm, E.J.; Fields, M.W.; Wall, J.; Stahl, D.; Hazen, T.C.; Keasling, J.D.; Arkin, A.P.; Zhou, J.

    2009-12-01T23:59:59.000Z

    The response of Desulfovibrio vulgaris Hildenborough to salt adaptation (long-term NaCl exposure) was examined by physiological, global transcriptional, and metabolite analyses. The growth of D. vulgaris was inhibited by high levels of NaCl, and the growth inhibition could be relieved by the addition of exogenous amino acids (e.g., glutamate, alanine, tryptophan) or yeast extract. Salt adaptation induced the expression of genes involved in amino acid biosynthesis and transport, electron transfer, hydrogen oxidation, and general stress responses (e.g., heat shock proteins, phage shock proteins, and oxidative stress response proteins). Genes involved in carbon metabolism, cell motility, and phage structures were repressed. Comparison of transcriptomic profiles of D. vulgaris responses to salt adaptation with those of salt shock (short-term NaCl exposure) showed some similarity as well as a significant difference. Metabolite assays showed that glutamate and alanine were accumulated under salt adaptation, suggesting that they may be used as osmoprotectants in D. vulgaris. A conceptual model is proposed to link the observed results to currently available knowledge for further understanding the mechanisms of D. vulgaris adaptation to elevated NaCl.

  12. Energetic Consequences of nitrite stress in Desulfovibrio vulgarisHildenborough, inferred from global transcriptional analysis

    SciTech Connect (OSTI)

    He, Qiang; Huang, Katherine H.; He, Zhili; Alm, Eric J.; Fields,Matthew W.; Hazen, Terry C.; Arkin, Adam P.; Wall, Judy D.; Zhou, Jizhong

    2005-11-03T23:59:59.000Z

    Many of the proteins that are candidates for bioenergetic pathways involved with sulfate respiration in Desulfovibrio spp. have been studied, but complete pathways and overall cell physiology remain to be resolved for many environmentally relevant conditions. In order to understand the metabolism of these microorganisms under adverse environmental conditions for improved bioremediation efforts, Desulfovibrio vulgaris Hildenborough was used as a model organism to study stress response to nitrite, an important intermediate in the nitrogen cycle. Previous physiological studies demonstrated that growth was inhibited by nitrite and that nitrite reduction was observed to be the primary mechanism of detoxification. Global transcriptional profiling with whole-genome microarrays revealed coordinated cascades of responses to nitrite in pathways of energy metabolism, nitrogen metabolism, oxidative stress response, and iron homeostasis. In agreement with previous observations, nitrite-stressed cells showed a decrease in the expression of genes encoding sulfate reduction functions in addition to respiratory oxidative phosphorylation and ATP synthase activity. Consequently, the stressed cells had decreased expression of the genes encoding ATP-dependent amino acid transporters and proteins involved in translation. Other genes up-regulated in response to nitrite include the genes in the Fur regulon, which is suggested to be involved in iron homeostasis, and genes in the Per regulon, which is predicted to be responsible for oxidative stress response.

  13. The Agrobacterium tumefaciens Transcription Factor BlcR Is Regulated via Oligomerization

    SciTech Connect (OSTI)

    Pan, Yi; Fiscus, Valena; Meng, Wuyi; Zheng, Zhida; Zhang, Lian-Hui; Fuqua, Clay; Chen, Lingling (IMCB-Singapore); (Indiana)

    2012-02-08T23:59:59.000Z

    The Agrobacterium tumefaciens BlcR is a member of the emerging isocitrate lyase transcription regulators that negatively regulates metabolism of {gamma}-butyrolactone, and its repressing function is relieved by succinate semialdehyde (SSA). Our crystal structure showed that BlcR folded into the DNA- and SSA-binding domains and dimerized via the DNA-binding domains. Mutational analysis identified residues, including Phe{sup 147}, that are important for SSA association; BlcR{sup F147A} existed as tetramer. Two BlcR dimers bound to target DNA and in a cooperative manner, and the distance between the two BlcR-binding sequences in DNA was critical for BlcR-DNA association. Tetrameric BlcR{sup F147A} retained DNA binding activity, and importantly, this activity was not affected by the distance separating the BlcR-binding sequences in DNA. SSA did not dissociate tetrameric BlcR{sup F147A} or BlcR{sup F147A}-DNA. As well as in the SSA-binding site, Phe{sup 147} is located in a structurally flexible loop that may be involved in BlcR oligomerization. We propose that SSA regulates BlcR DNA-binding function via oligomerization.

  14. Crystal structure of the C-terminal domain of the RAP74 subunit of human transcription factor IIF

    SciTech Connect (OSTI)

    Kamada, Katsuhiko; De Angelis, Jacqueline; Roeder, Robert G.; Burley, Stephen K. (Rockefeller)

    2012-12-13T23:59:59.000Z

    The x-ray structure of a C-terminal fragment of the RAP74 subunit of human transcription factor (TF) IIF has been determined at 1.02-{angstrom} resolution. The {alpha}/{beta} structure is strikingly similar to the globular domain of linker histone H5 and the DNA-binding domain of hepatocyte nuclear factor 3{gamma} (HNF-3{gamma}), making it a winged-helix protein. The surface electrostatic properties of this compact domain differ significantly from those of bona fide winged-helix transcription factors (HNF-3{gamma} and RFX1) and from the winged-helix domains found within the RAP30 subunit of TFIIF and the {beta} subunit of TFIIE. RAP74 has been shown to interact with the TFIIF-associated C-terminal domain phosphatase FCP1, and a putative phosphatase binding site has been identified within the RAP74 winged-helix domain.

  15. The transcription factor LEF-1 induces an epithelial–mesenchymal transition in MDCK cells independent of ?-catenin

    SciTech Connect (OSTI)

    Kobayashi, Wakako; Ozawa, Masayuki, E-mail: mozawa@m.kufm.kagoshima-u.ac.jp

    2013-12-06T23:59:59.000Z

    Highlights: •The transcription factor LEF-1 induces an EMT in MDCK cells. •A mutant LEF-1 that cannot interact with ?-catenin retained the ability. •The nuclear function of ?-catenin was not necessary for the LEF-1-induced EMT. •The mRNA levels of Slug, ZEB1, and ZEB2 increased significantly in these cells. -- Abstract: The epithelial–mesenchymal transition (EMT), a key process in the tumor metastatic cascade, is characterized by the loss of cell–cell junctions and cell polarity, as well as the acquisition of migratory and invasive properties. LEF-1 is a member of the lymphoid enhancer-binding factor/T-cell factor (LEF/TCF) family of DNA-binding transcription factors, which interact with nuclear ?-catenin and act as central transcriptional mediators of Wnt signaling. To investigate the role of LEF-1 in EMT, we generated stable LEF-1 transfectants using MDCK cells. The transfectants had a spindle-shaped mesenchymal morphology, and enhanced migration and invasiveness relative to control cells. These EMT changes were accompanied by the downregulation of an epithelial marker protein, E-cadherin, and the upregulation of mesenchymal marker proteins, vimentin and N-cadherin. Consistent with these observations, the mRNA levels of Slug, ZEB1, and ZEB2—EMT-related transcription factors—increased significantly. Although the N-terminally deleted mutant LEF-1 cannot interact with ?-catenin, it retained the ability to induce EMT. Consistent with these observations, neither the expression of a dominant negative ?-catenin/engrailed chimera, nor the expression of a cytoplasmic domain of E-cadherin that sequesters ?-catenin from binding to LEF/TCF, reversed LEF-1-induced EMT. Together, these data indicated that the nuclear function of ?-catenin was not necessary for the induction of Slug, ZEB1, and ZEB2 expression leading to EMT.

  16. HPV-18 E2circumflexE4 chimera: 2 new spliced transcripts and proteins induced by keratinocyte differentiation

    SciTech Connect (OSTI)

    Tan, Chye Ling [Papillomavirus Regulation and Cancer, Institute of Medical Biology, Agency for Science, Technology and Research (A-STAR), Biopolis, 8A Biomedical Grove, Immunos, Singapore 138648 (Singapore)] [Papillomavirus Regulation and Cancer, Institute of Medical Biology, Agency for Science, Technology and Research (A-STAR), Biopolis, 8A Biomedical Grove, Immunos, Singapore 138648 (Singapore); Gunaratne, Jayantha [Mass Spectrometry and Systems Biology Laboratory, Institute of Molecular and Cell Biology, A-STAR, Biopolis, 61 Biopolis Drive, Proteos, Singapore 138673 (Singapore)] [Mass Spectrometry and Systems Biology Laboratory, Institute of Molecular and Cell Biology, A-STAR, Biopolis, 61 Biopolis Drive, Proteos, Singapore 138673 (Singapore); Lai, Deborah [Papillomavirus Regulation and Cancer, Institute of Medical Biology, Agency for Science, Technology and Research (A-STAR), Biopolis, 8A Biomedical Grove, Immunos, Singapore 138648 (Singapore)] [Papillomavirus Regulation and Cancer, Institute of Medical Biology, Agency for Science, Technology and Research (A-STAR), Biopolis, 8A Biomedical Grove, Immunos, Singapore 138648 (Singapore); Carthagena, Laetitia [UMR-S996, Universite Paris-Sud 11, 32 rue des Carnets, 92140 Clamart (France)] [UMR-S996, Universite Paris-Sud 11, 32 rue des Carnets, 92140 Clamart (France); Wang, Qian [MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London N10 3UE (United Kingdom)] [MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London N10 3UE (United Kingdom); Xue, Yue Zhen; Quek, Ling Shih [Papillomavirus Regulation and Cancer, Institute of Medical Biology, Agency for Science, Technology and Research (A-STAR), Biopolis, 8A Biomedical Grove, Immunos, Singapore 138648 (Singapore)] [Papillomavirus Regulation and Cancer, Institute of Medical Biology, Agency for Science, Technology and Research (A-STAR), Biopolis, 8A Biomedical Grove, Immunos, Singapore 138648 (Singapore); Doorbar, John [MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London N10 3UE (United Kingdom)] [MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London N10 3UE (United Kingdom); Bachelerie, Francoise [UMR-S996, Universite Paris-Sud 11, 32 rue des Carnets, 92140 Clamart (France)] [UMR-S996, Universite Paris-Sud 11, 32 rue des Carnets, 92140 Clamart (France); Thierry, Francoise, E-mail: francoise.thierry@imb.a-star.edu.sg [Papillomavirus Regulation and Cancer, Institute of Medical Biology, Agency for Science, Technology and Research (A-STAR), Biopolis, 8A Biomedical Grove, Immunos, Singapore 138648 (Singapore)] [Papillomavirus Regulation and Cancer, Institute of Medical Biology, Agency for Science, Technology and Research (A-STAR), Biopolis, 8A Biomedical Grove, Immunos, Singapore 138648 (Singapore); Bellanger, Sophie, E-mail: sophie.bellanger@imb.a-star.edu.sg [Papillomavirus Regulation and Cancer, Institute of Medical Biology, Agency for Science, Technology and Research (A-STAR), Biopolis, 8A Biomedical Grove, Immunos, Singapore 138648 (Singapore)] [Papillomavirus Regulation and Cancer, Institute of Medical Biology, Agency for Science, Technology and Research (A-STAR), Biopolis, 8A Biomedical Grove, Immunos, Singapore 138648 (Singapore)

    2012-07-20T23:59:59.000Z

    The Human Papillomavirus (HPV) E4 is known to be synthesized as an E1circumflexE4 fusion resulting from splice donor and acceptor sites conserved across HPV types. Here we demonstrate the existence of 2 HPV-18 E2circumflexE4 transcripts resulting from 2 splice donor sites in the 5 Prime part of E2, while the splice acceptor site is the one used for E1circumflexE4. Both E2circumflexE4 transcripts are up-regulated by keratinocyte differentiation in vitro and can be detected in clinical samples containing low-grade HPV-18-positive cells from Pap smears. They give rise to two fusion proteins in vitro, E2circumflexE4-S and E2circumflexE4-L. Whereas we could not differentiate E2circumflexE4-S from E1circumflexE4 in vivo, E2circumflexE4-L could be formally identified as a 23 kDa protein in raft cultures in which the corresponding transcript was also found, and in a biopsy from a patient with cervical intraepithelial neoplasia stage I-II (CINI-II) associated with HPV-18, demonstrating the physiological relevance of E2circumflexE4 products.

  17. PIAS proteins are involved in the SUMO-1 modification, intracellular translocation and transcriptional repressive activity of RET finger protein

    SciTech Connect (OSTI)

    Matsuura, Tetsuo [Department of Pathology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550 (Japan); Department of Gastroenterology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550 (Japan); Shimono, Yohei [Department of Pathology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550 (Japan); Kawai, Kumi [Department of Molecular Pathology, Center for Neurological Diseases and Cancer, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550 (Japan); Murakami, Hideki [Department of Pathology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550 (Japan); Urano, Takeshi [Department of Biochemistry, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550 (Japan); Niwa, Yasumasa [Department of Gastroenterology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550 (Japan); Goto, Hidemi [Department of Gastroenterology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550 (Japan); Takahashi, Masahide [Department of Pathology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550 (Japan) and Department of Molecular Pathology, Center for Neurological Diseases and Cancer, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550 (Japan)]. E-mail: mtakaha@med.nagoya-u.ac.jp

    2005-08-01T23:59:59.000Z

    Ret finger protein (RFP) is a nuclear protein that is highly expressed in testis and in various tumor cell lines. RFP functions as a transcriptional repressor and associates with Enhancer of Polycomb 1 (EPC1), a member of the Polycomb group proteins, and Mi-2{beta}, a main component of the nucleosome remodeling and deacetylase (NuRD) complex. We show that RFP binds with PIAS (protein inhibitor of activated STAT) proteins, PIAS1, PIAS3, PIASx{alpha} and PIASy at their carboxyl-terminal region and is covalently modified by SUMO-1 (sumoylation). PIAS proteins enhance the sumoylation of RFP in a dose-dependent manner and induce the translocation of RFP into nuclear bodies reminiscent of the PML bodies. In addition, co-expression of PIAS proteins or SUMO-1 strengthened the transcriptional repressive activity of RFP. Finally, our immunohistochemical results show that RFP, SUMO-1 and PIASy localize in a characteristic nuclear structure juxtaposed with the inner nuclear membrane (XY body) of primary spermatocytes in mouse testis. These results demonstrate that the intracellular location and the transcriptional activity of RFP are modified by PIAS proteins which possess SUMO E3 ligase activities and suggest that they may play a co-operative role in spermatogenesis.

  18. AAV-mediated delivery of the transcription factor XBP1s into the striatum reduces mutant Huntingtin aggregation in a mouse model of Huntington's disease

    SciTech Connect (OSTI)

    Zuleta, Amparo [Biomedical Neuroscience Institute, Faculty of Medicine, University of Chile, Santiago (Chile) [Biomedical Neuroscience Institute, Faculty of Medicine, University of Chile, Santiago (Chile); Center for Molecular Studies of the Cell, Institute of Biomedical Sciences, University of Chile, Santiago (Chile); Vidal, Rene L. [Biomedical Neuroscience Institute, Faculty of Medicine, University of Chile, Santiago (Chile) [Biomedical Neuroscience Institute, Faculty of Medicine, University of Chile, Santiago (Chile); Neurounion Biomedical Foundation, Santiago (Chile); Armentano, Donna; Parsons, Geoffrey [Department of Molecular Biology, Genzyme Corporation, 49 New York Avenue, Framingham, MA 01701 (United States)] [Department of Molecular Biology, Genzyme Corporation, 49 New York Avenue, Framingham, MA 01701 (United States); Hetz, Claudio, E-mail: chetz@med.uchile.cl [Biomedical Neuroscience Institute, Faculty of Medicine, University of Chile, Santiago (Chile) [Biomedical Neuroscience Institute, Faculty of Medicine, University of Chile, Santiago (Chile); Center for Molecular Studies of the Cell, Institute of Biomedical Sciences, University of Chile, Santiago (Chile); Department of Immunology and Infectious Diseases, Harvard School of Public Health, 651 Huntington Av., Boston, MA 02446 (United States); Neurounion Biomedical Foundation, Santiago (Chile)

    2012-04-13T23:59:59.000Z

    Highlights: Black-Right-Pointing-Pointer The contribution of ER stress to HD has not been directly addressed. Black-Right-Pointing-Pointer Expression of XBP1s using AAVs decreases Huntingtin aggregation in vivo. Black-Right-Pointing-Pointer We describe a new in vivo model of HD based on the expression of a large fragment of mHtt-RFP. -- Abstract: Huntington's disease (HD) is caused by mutations that expand a polyglutamine region in the amino-terminal domain of Huntingtin (Htt), leading to the accumulation of intracellular inclusions and progressive neurodegeneration. Recent reports indicate the engagement of endoplasmic reticulum (ER) stress responses in human HD post mortem samples and animal models of the disease. Adaptation to ER stress is mediated by the activation of the unfolded protein response (UPR), an integrated signal transduction pathway that attenuates protein folding stress by controlling the expression of distinct transcription factors including X-Box binding protein 1 (XBP1). Here we targeted the expression of XBP1 on a novel viral-based model of HD. We delivered an active form of XBP1 locally into the striatum of adult mice using adeno-associated vectors (AAVs) and co-expressed this factor with a large fragment of mutant Htt as a fusion protein with RFP (Htt588{sup Q95}-mRFP) to directly visualize the accumulation of Htt inclusions in the brain. Using this approach, we observed a significant reduction in the accumulation of Htt588{sup Q95}-mRFP intracellular inclusion when XBP1 was co-expressed in the striatum. These results contrast with recent findings indicating a protective effect of XBP1 deficiency in neurodegeneration using knockout mice, and suggest a potential use of gene therapy strategies to manipulate the UPR in the context of HD.

  19. BPA guide to finding your property online - I-5 Corridor Reinforcement...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    to finding your property online In June 2014, BPA created an updated version of the interactive map that reflects recent adjustments to proposed transmission tower and access road...

  20. Comparative Corporate Governance: Findings from a Workshop, March 14-15, 2008

    E-Print Network [OSTI]

    Huberty, Mark; Newsome, Akasemi; Street, Alex; Ziegler, J. Nicholas

    2008-01-01T23:59:59.000Z

    of Chicago, ‘‘Corporate Governance: History withoutComparative Corporate Governance Findings from a Workshopon Comparative Corporate Governance, March 14-15, 2008

  1. A procedure for finding the k-th power of a matrix

    E-Print Network [OSTI]

    Branko Malesevic; Ivana Jovovic

    2007-04-08T23:59:59.000Z

    We give a new procedure in Maple for finding the k-th power of a martix. The algorithm is based on the article [1].

  2. New findings show some Type Ia supernovae linked to novae | EurekAlert! Science News

    E-Print Network [OSTI]

    ... findings show some Type Ia supernovae linked to novae ... least some thermonuclear (Type Ia) supernovae come from a recurrent nova ... originators of other Type Ia supernovae . ...

  3. How to Find More Supernovae with Less Work: Object Classification Techniques for Difference Imaging

    E-Print Network [OSTI]

    Bailey, Stephen; Aragon, Cecilia; Romano, Raquel; Thomas, Rollin C.; Weaver, Benjamin A.; Wong, Daniel

    2007-01-01T23:59:59.000Z

    How to Find More Supernovae with Less Work: Object Classi?methods: statistical — supernovae: general — techniques:for objects such as supernovae, active galactic nuclei,

  4. Report on Hydrogen Storage Panel Findings in DOE-BES Sponsored...

    Broader source: Energy.gov (indexed) [DOE]

    Basic Energy Sciences Basic Energy Sciences DOEEERE Hydrogen Storage Pre-Solicitation Meeting, June 19, 2003 Report on Hydrogen Storage Panel Findings in Report on Hydrogen...

  5. HCFC1 is a common component of active human CpG-island promoters and coincides with ZNF143, THAP11, YY1, and GABP transcription factor occupancy

    E-Print Network [OSTI]

    JnBaptiste, Courtney Kenneil

    In human transcriptional regulation, DNA-sequence-specific factors can associate with intermediaries that orchestrate interactions with a diverse set of chromatin-modifying enzymes. One such intermediary is HCFC1 (also ...

  6. Transcript of See Action and Technical Assistance Program Webcast: Energy Audit and Retro-Commissioning Policies for Public and Commercial Buildings

    Broader source: Energy.gov [DOE]

    U.S. Department of Energy Office (DOE) of Energy Efficiency and Renewable Energy (EERE) transcript of See Action and Technical Assistance Program Webcast: Energy Audit and Retro-Commissioning Policies for Public and Commercial Buildings.

  7. Uncovering Transcriptional Circuits in Arabidoposis by Functional Genomics (2013 DOE JGI Genomics of Energy and Environment 8th Annual User Meeting)

    SciTech Connect (OSTI)

    Pruneda-Paz, Jose [UC San Diego

    2013-03-01T23:59:59.000Z

    Jose Pruneda-Paz of UC San Diego on "Uncovering Transcriptional Circuits in Arabidopsis by Functional Genomics" at the 8th Annual Genomics of Energy & Environment Meeting in Walnut Creek, Calif.

  8. Structural Basis of Low-Affinity Nickel Binding to the Nickel-Responsive Transcription Factor NikR from Escherichia coli

    E-Print Network [OSTI]

    Phillips, Christine M.

    Escherichia coli NikR regulates cellular nickel uptake by binding to the nik operon in the presence of nickel and blocking transcription of genes encoding the nickel uptake transporter. NikR has two binding affinities for ...

  9. Toxoplasma gondii Clonal Strains All Inhibit STAT1 Transcriptional Activity but Polymorphic Effectors Differentially Modulate IFN gamma Induced Gene Expression and STAT1 Phosphorylation

    E-Print Network [OSTI]

    Rosowski, Emily Elizabeth

    Host defense against the parasite Toxoplasma gondii requires the cytokine interferon-gamma (IFN?). However, Toxoplasma inhibits the host cell transcriptional response to IFN?, which is thought to allow the parasite to ...

  10. Experimental Definition and Validation of Protein Coding Transcripts in Chlamydomonas reinhardtii

    SciTech Connect (OSTI)

    Kourosh Salehi-Ashtiani; Jason A. Papin

    2012-01-13T23:59:59.000Z

    Algal fuel sources promise unsurpassed yields in a carbon neutral manner that minimizes resource competition between agriculture and fuel crops. Many challenges must be addressed before algal biofuels can be accepted as a component of the fossil fuel replacement strategy. One significant challenge is that the cost of algal fuel production must become competitive with existing fuel alternatives. Algal biofuel production presents the opportunity to fine-tune microbial metabolic machinery for an optimal blend of biomass constituents and desired fuel molecules. Genome-scale model-driven algal metabolic design promises to facilitate both goals by directing the utilization of metabolites in the complex, interconnected metabolic networks to optimize production of the compounds of interest. Using Chlamydomonas reinhardtii as a model, we developed a systems-level methodology bridging metabolic network reconstruction with annotation and experimental verification of enzyme encoding open reading frames. We reconstructed a genome-scale metabolic network for this alga and devised a novel light-modeling approach that enables quantitative growth prediction for a given light source, resolving wavelength and photon flux. We experimentally verified transcripts accounted for in the network and physiologically validated model function through simulation and generation of new experimental growth data, providing high confidence in network contents and predictive applications. The network offers insight into algal metabolism and potential for genetic engineering and efficient light source design, a pioneering resource for studying light-driven metabolism and quantitative systems biology. Our approach to generate a predictive metabolic model integrated with cloned open reading frames, provides a cost-effective platform to generate metabolic engineering resources. While the generated resources are specific to algal systems, the approach that we have developed is not specific to algae and can be readily expanded to other microbial systems as well as higher plants and animals.

  11. Findings from the 2004 Fully Automated Demand Response Tests in Large

    E-Print Network [OSTI]

    LBNL-58178 Findings from the 2004 Fully Automated Demand Response Tests in Large Facilities M;Findings from the 2004 Fully Automated Demand Response Tests in Large Facilities September 7, 2005 Mary Ann Manager Dave Michel Contract 500-03-026 Sponsored by the California Energy Commission PIER Demand Response

  12. Eye Finding via Face Detection for a Foveated, Active Vision System Brian Scassellati

    E-Print Network [OSTI]

    Eye Finding via Face Detection for a Foveated, Active Vision System Brian Scassellati 545 Technology Square MIT Artificial Intelligence Lab Cambridge, MA, 02139, USA scaz@ai.mit.edu Abstract Eye finding is the first step toward building a ma- chine that can recognize social cues, like eye contact

  13. Eye Finding via Face Detection for a Foveated, Active Vision System Brian Scassellati

    E-Print Network [OSTI]

    Eye Finding via Face Detection for a Foveated, Active Vision System Brian Scassellati 545 Technology Square MIT Artificial Intelligence Lab Cambridge, MA, 02139, USA scaz@ai.mit.edu Abstract Eye finding is the first step toward building a ma­ chine that can recognize social cues, like eye contact

  14. FINDINGS SECTION 16 FISH AND WILDLIFE PROGRAM 16-1 September 13, 1995

    E-Print Network [OSTI]

    FINDINGS SECTION 16 FISH AND WILDLIFE PROGRAM 16-1 September 13, 1995 1 Section 162 3 Findings on the Recommendations for Amendments to the4 Resident Fish and Wildlife Portions of the 1994 Fish and Wildlife Program5 and Response to Comments6 September 13, 19957 8 9 In late 1994 the Council requested that fish and wildlife

  15. MRI FINDINGS AND CLINICAL OUTCOME IN 45 DIVERS WITH SPINAL CORD DECOMPRESSION SICKNESS

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    1 MRI FINDINGS AND CLINICAL OUTCOME IN 45 DIVERS WITH SPINAL CORD DECOMPRESSION SICKNESS Emmanuel clinical outcome in divers with spinal cord DCS. The presence of medullary compressive factors was conducted to evaluate the relationship between spinal cord lesions on MRI and clinical findings in divers

  16. Relevance of Upstream Open Reading Frames in eIF5 and Inositol-3-Phosphate Synthase Transcripts in Neurospora crassa

    E-Print Network [OSTI]

    Bennett, Christopher

    2011-04-25T23:59:59.000Z

    understanding of the processes in common for all eukaryotic organisms. 4 CHAPTER II METHODS The sequences of the uORFs in question can be found on-line at www.ncbi.nlm.nih.gov at the 5? end of I3PS, numbered NCU06666, and eIF5, numbered NCU00366. pJW201...April 2011 Majors: Biology Genetics RELEVANCE OF UPSTREAM OPEN READING FRAMES IN eIF5 AND INOSITOL-3-PHOSPHATE SYNTHASE TRANSCRIPTS IN NEUROSPORA CRASSA Submitted to the Office of Undergraduate Research Texas A&M University...

  17. DOE/EA-1905 FINDING OF NO SIGNIFICANT IMPACT DEPARTMENT OF ENERGY...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    905 FINDING OF NO SIGNIFICANT IMPACT DEPARTMENT OF ENERGY FUNDING FOR CITY OF CARLSBAD, NM DOUBLE EAGLE WATER SYSTEM AGENCY: U.S. Department of Energy Carlsbad Field Office (DOE...

  18. A Cone Jet-Finding Algorithm for Heavy-Ion Collisions at LHC Energies

    E-Print Network [OSTI]

    2008-01-01T23:59:59.000Z

    A Cone Jet-Finding Algorithm for Heavy-Ion Collisions at LHCf CERN, Abstract Standard jet ?nding techniques used indescribes a modi?ed cone-type jet ?nding algorithm developed

  19. Smithsonian/NASA ADS Physics/Geophysics Abstract Service Find Similar Abstracts (with default settings below)

    E-Print Network [OSTI]

    Mojahedi, Mohammad

    Smithsonian/NASA ADS Physics/Geophysics Abstract Service · Find Similar Abstracts (with default | Query Form | Preferences | HELP | FAQ Physics/Geophysics arXiv e-prints Send Query Reset #12;

  20. MA 15800 Exam 3 Spring 2015 1. Find the exact solution using ...

    E-Print Network [OSTI]

    2015-04-21T23:59:59.000Z

    There is a 15 inch wiper blade attached by a 4 inch arm at the center of the base of the window. If the arm rotates. 130o, find the area wiped out by the blade to ...

  1. Abstract. Clinical, epidemiological and experimental findings have provided evidence supporting a role of free radicals in

    E-Print Network [OSTI]

    Abstract. Clinical, epidemiological and experimental findings have provided evidence supporting conditions of stress, contributing widely to cancer development. Clinical, epidemiological and experimental in experimental, clinical and epidemio- logical studies for cancer chemoprevention. It has been shown

  2. Numerical method for finding decoherence-free subspaces and its applications

    E-Print Network [OSTI]

    Wang, Xiaoting

    In this work, inspired by the study of semidefinite programming for block-diagonalizing matrix *-algebras, we propose an algorithm that can find the algebraic structure of decoherence-free subspaces (DFS's) for a given ...

  3. 1 Finding the Structure of Documents 1 1.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1

    E-Print Network [OSTI]

    Favre, Benoit

    Contents 1 Finding the Structure of Documents 1 1.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 1.1.1 Sentence Boundary Detection . . . . . . . . . . . . . . . . 2 1.1.2 Topic Boundary Detection . . . . . . . . . . . . . . . . . . 4 1.2 Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 1.2.1 Generative

  4. Math 13900 YouTube Quiz 2013 Find a video on YouTube that ...

    E-Print Network [OSTI]

    Roames, Renee S

    2013-01-02T23:59:59.000Z

    Find a video on YouTube that addresses the idea of symmetry – either for 2 dimensions or 3 as we have been studying in lessons 9 and 10. The video should be ...

  5. New Materials In The Mn-Cr-Co-O-System Ditte Find, Roskilde University Center

    E-Print Network [OSTI]

    Östby, Finn Willy Poulsen and Peter Vang Hendriksen Risř National Laboratory - Fuel Cells and Solid the conduction and makes it necessary to optimize the composition of the steel or find alternative materials

  6. Finding Creativity in an Artificial Artist David Norton, Derrall Heath and Dan Ventura

    E-Print Network [OSTI]

    Martinez, Tony R.

    Finding Creativity in an Artificial Artist David Norton, Derrall Heath and Dan Ventura Computer Science Department Brigham Young University dnorton@byu.edu, dheath@byu.edu, ventura@cs.byu.edu Abstract

  7. 1 SERU Findings | Office for Public Engagement engagement.umn.edu

    E-Print Network [OSTI]

    Amin, S. Massoud

    service initiatives across the University's five campuses, as well as to capture the impact of our they find these experiences valuable to their overall education at the University. The data we have collected also provides us with an opportu

  8. Effectiveness of bomber deployed autonomous airborne vehicles in finding rail mobile SS-24s

    SciTech Connect (OSTI)

    Abey, A.E.; Erickson, S.A.; Norquist, P.D.

    1990-08-01T23:59:59.000Z

    Computer simulation predictions of the effectiveness of autonomous airborne vehicles in finding rail mobile SS-24s are presented. Effectiveness is discussed for several autonomous airborne vehicle endurances and survivabilities for the search area southwest of Moscow. The effect of where the Soviets place the SS-24s on the rail network was also investigated. The simulation predicts significant variations in the ability of a multi-autonomous airborne vehicle system to find SS-24s with these parameters. 12 figs., 1 tab.

  9. Heat shock factor 1 upregulates transcription of Epstein-Barr Virus nuclear antigen 1 by binding to a heat shock element within the BamHI-Q promoter

    SciTech Connect (OSTI)

    Wang, Feng-Wei [The State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-Sen University, Guangzhou (China)] [The State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-Sen University, Guangzhou (China); Wu, Xian-Rui [Department of Surgery, Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou (China)] [Department of Surgery, Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou (China); Liu, Wen-Ju; Liao, Yi-Ji [The State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-Sen University, Guangzhou (China)] [The State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-Sen University, Guangzhou (China); Lin, Sheng [Laboratory of Integrated Biosciences, School of Life Science, Sun Yat-sen University, Guangzhou (China)] [Laboratory of Integrated Biosciences, School of Life Science, Sun Yat-sen University, Guangzhou (China); Zong, Yong-Sheng; Zeng, Mu-Sheng; Zeng, Yi-Xin [The State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-Sen University, Guangzhou (China)] [The State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-Sen University, Guangzhou (China); Mai, Shi-Juan, E-mail: maishj@sysucc.org.cn [The State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-Sen University, Guangzhou (China)] [The State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-Sen University, Guangzhou (China); Xie, Dan, E-mail: xied@mail.sysu.edu.cn [The State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-Sen University, Guangzhou (China)] [The State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-Sen University, Guangzhou (China)

    2011-12-20T23:59:59.000Z

    Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA1) is essential for maintenance of the episome and establishment of latency. In this study, we observed that heat treatment effectively induced EBNA1 transcription in EBV-transformed B95-8 and human LCL cell lines. Although Cp is considered as the sole promoter used for the expression of EBNA1 transcripts in the lymphoblastoid cell lines, the RT-PCR results showed that the EBNA1 transcripts induced by heat treatment arise from Qp-initiated transcripts. Using bioinformatics, a high affinity and functional heat shock factor 1 (HSF1)-binding element within the - 17/+4 oligonucleotide of the Qp was found, and was determined by electrophoretic mobility shift assay and chromatin immunoprecipitation assay. Moreover, heat shock and exogenous HSF1 expression induced Qp activity in reporter assays. Further, RNA interference-mediated HSF1 gene silencing attenuated heat-induced EBNA1 expression in B95-8 cells. These results provide evidence that EBNA1 is a new target for the transcription factor HSF1.

  10. The mitochondrial fatty acid synthesis (mtFASII) pathway is capable of mediating nuclear-mitochondrial cross talk through the PPAR system of transcriptional activation

    SciTech Connect (OSTI)

    Parl, Angelika; Mitchell, Sabrina L.; Clay, Hayley B.; Reiss, Sara; Li, Zhen; Murdock, Deborah G., E-mail: deborah.murdock@vanderbilt.edu

    2013-11-15T23:59:59.000Z

    Highlights: •The function of the mitochondria fatty acid synthesis pathway is partially unknown. •Overexpression of the pathway causes transcriptional activation through PPARs. •Knock down of the pathway attenuates that activation. •The last enzyme in the pathway regulates its own transcription. •Products of the mtFASII pathway are able to drive nuclear transcription. -- Abstract: Mammalian cells contain two fatty acid synthesis pathways, the cytosolic FASI pathway, and the mitochondrial FASII pathway. The selection behind the conservation of the mitochondrial pathway is not completely understood, given the presence of the cytosolic FAS pathway. In this study, we show through heterologous gene reporter systems and PCR-based arrays that overexpression of MECR, the last step in the mtFASII pathway, causes modulation of gene expression through the PPAR pathway. Electromobility shift assays (EMSAs) demonstrate that overexpression of MECR causes increased binding of PPARs to DNA, while cell fractionation and imaging studies show that MECR remains localized to the mitochondria. Interestingly, knock down of the mtFASII pathway lessens the effect of MECR on this transcriptional modulation. Our data are most consistent with MECR-mediated transcriptional activation through products of the mtFASII pathway, although we cannot rule out MECR acting as a coactivator. Further investigation into the physiological relevance of this communication will be necessary to better understand some of the phenotypic consequences of deficits in this pathway observed in animal models and human disease.

  11. A Simple Method for Finding the Scattering Coefficients of Quantum Graphs

    E-Print Network [OSTI]

    Seth S. Cottrell

    2015-03-01T23:59:59.000Z

    Quantum walks are roughly analogous to classical random walks, and like classical walks they have been used to find new (quantum) algorithms. When studying the behavior of large graphs or combinations of graphs it is useful to find the response of a subgraph to signals of different frequencies. In so doing we can replace an entire subgraph with a single vertex with frequency dependent scattering coefficients. In this paper a simple technique for quickly finding the scattering coefficients of any quantum graph will be presented. These scattering coefficients can be expressed entirely in terms of the characteristic polynomial of the graph's time step operator. Moreover, with these in hand we can easily derive the "impulse response" which is the key to predicting the response of a graph to any signal. This gives us a powerful set of tools for rapidly understanding the behavior of graphs or for reducing a large graph into its constituent subgraphs regardless of how they are connected.

  12. Local-global alignment for finding 3D similarities in protein structures

    DOE Patents [OSTI]

    Zemla, Adam T. (Brentwood, CA)

    2011-09-20T23:59:59.000Z

    A method of finding 3D similarities in protein structures of a first molecule and a second molecule. The method comprises providing preselected information regarding the first molecule and the second molecule. Comparing the first molecule and the second molecule using Longest Continuous Segments (LCS) analysis. Comparing the first molecule and the second molecule using Global Distance Test (GDT) analysis. Comparing the first molecule and the second molecule using Local Global Alignment Scoring function (LGA_S) analysis. Verifying constructed alignment and repeating the steps to find the regions of 3D similarities in protein structures.

  13. Finding investment criteria for monitoring based commissioning (MBCx) services- focus group evidence from Finland

    E-Print Network [OSTI]

    Laitala, A.

    2012-01-01T23:59:59.000Z

    Finding?investment?criteria?for? monitoring?based?commissioning? (MBCx)?services? ? focus?group?evidence?from?Finland Ari?Laitala Aalto?University,?Finland School?of?Engineering Department?of?Real?Estate,?Planning?and?Geoinformatics 12...?making?criterion ? In?the?line?with?the?earlier?research?findings? 11/12/2012 Ari?Laitala?ICEBO?2012 22 Criticism ? Evidence?power?of?the?focus?group?discussions ? On?the?other?hand?it?is?believed?that?many?of?the? leading?MBCx experts?(Finland...

  14. EA-1605: Revised Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels DataDepartment of Energy Your Density Isn't Your Destiny:Revised Finding of No53197E TDrew Bittner56:1: Mitigation Action3:Finding of

  15. EA-1849-S1: Finding of No Significant Impact | Department of Energy

    Energy Savers [EERE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directed off Energy.gov. Are you0andEnergyGlobal NuclearofCommunication |DoesFinding of NoFindingS1:

  16. A Correlated Motif Approach for Finding Short Linear Motifs from Protein-Protein Interaction Data

    E-Print Network [OSTI]

    Sung, Wing-Kin Ken"

    interactions. We adopted the (l, d)-motif model and formulate finding the corre- lated motifs as an (l, d algorithm, D-STAR to solve this problem. Evaluation on extensive simulated data showed that our approach- ill@comp.nus.edu.sg. Background An important class of interaction switches for bio- logical circuits

  17. Finding the Energy Efficient Curve: Gate Sizing for Minimum Power under Delay Constraints

    E-Print Network [OSTI]

    Kolodny, Avinoam

    Finding the Energy Efficient Curve: Gate Sizing for Minimum Power under Delay Constraints Yoni in a fast circuit by the same factor does not yield an energy-efficient design, and we characterize efficient. A design implementation is considered to be energy efficient when it has the highest performance

  18. Final Exam/Fall 1998 Math 351 Name (10) 1. Find a 2 × 2 matrix A ...

    E-Print Network [OSTI]

    1910-10-62T23:59:59.000Z

    ... be a 2 × 2 matrix, Q1 = [1, -1]t and Q2 = [3, -2]t. Assume that. AQ1 = 4Q1 , AQ2 = 9Q2. (i) Find an invertible matrix Q and a diagonal matrix D such that A = QDQ.

  19. Methodology for Carbon Footprint in Forestry Findings and Ways of Improvement

    E-Print Network [OSTI]

    Boyer, Edmond

    ; in our application case it was 2008. 2.2 Bibliography A lot of studies focus on carbon sequestrationMethodology for Carbon Footprint in Forestry Findings and Ways of Improvement Gabriel Chauvet1 Auvergne, Site de Marmilhat, F-63370 Lempdes Abstract. Classic methodologies for carbon footprint are made

  20. A globally convergent method for finding all steady-state solutions of distillation columns

    E-Print Network [OSTI]

    Neumaier, Arnold

    A globally convergent method for finding all steady-state solutions of distillation columns Ali distillation column with 7 steady-states show the robustness of the method. No published software known to the steady-state model of distillation columns as it returns the original system as a single large block

  1. Methane Storage in Metal-Organic Frameworks: Current Records, Surprise Findings, and Challenges

    E-Print Network [OSTI]

    Methane Storage in Metal-Organic Frameworks: Current Records, Surprise Findings, and Challenges to concerns over national and regional energy security, ground-level air quality, and climate change. While challenge is mass- and volume-efficient, ambient-temperature storage and delivery. One potential solution

  2. eVADER: A Perceptual Approach to Finding Minimum Warning Sound Requirements for Quiet Cars.

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    , such as hybrid and electric vehicles, to pedestrians has become an important issue for public policy [1], car a localization paradigm to test the detectability of hybrid and internal combustion cars by measuring listenereVADER: A Perceptual Approach to Finding Minimum Warning Sound Requirements for Quiet Cars. Ryan

  3. NEW FINDINGS IN THE MARKET MICROSTRUCTURE OF OVER-THE-COUNTER MARKETS

    E-Print Network [OSTI]

    Sadeh, Norman M.

    -exempt) bond market. However, the implications on price formation, price discovery and dealer's market power. In the first chapter "Price Clustering and Dealer's Market Power", I find that prices, yields and markups is statistically verified in the data. Dealer's profit is indeed monotonically increasing with the pricing grid

  4. CALIFORNIA ENVIRONMENTAL QUALITY ACT FINDINGS IN CONNECTION WITH THE AMENDMENT TO THE 2003 LRDP AND

    E-Print Network [OSTI]

    California at Davis, University of

    2003 LONG RANGE DEVELOPMENT PLAN FINAL ENVIRONMENTAL IMPACT REPORT The findings set forth below") of Addendum #1 to the Final Environmental Impact Report ("Final EIR") for the University of California, Davis. The Final EIR consists of the May 2003 Draft Environmental Impact Report ("Draft EIR") and the November 2003

  5. Hillclimbing finds random planted bisections Ted Carson and Russell Impagliazzo \\Lambda

    E-Print Network [OSTI]

    Impagliazzo, Russell

    Hill­climbing finds random planted bisections Ted Carson and Russell Impagliazzo \\Lambda Computer problem on instances drawn from the ``planted bisection'' ran­ dom graph model, Gn;p;q , previously the planted bisection with high probability if p \\Gamma q =\\Omega \\Gamma n \\Gamma1=2 ln 4 n \\Delta . For dense

  6. taBLe of Contents 2 Find a Job or Internship

    E-Print Network [OSTI]

    Hone, James

    spring 2013 program of events #12;taBLe of Contents 2 Find a Job or Internship 4 All Students, 2013 Deadline to accept internship offers from previous summer internships March 15, 2013 Deadline to accept internship offers received during Spring Semester OCR Employer Info Sessions Employer Info

  7. Optimization on Solar Panels: Finding the Optimal Output Brian Y. Lu

    E-Print Network [OSTI]

    Lavaei, Javad

    Optimization on Solar Panels: Finding the Optimal Output Brian Y. Lu January 1, 2013 1 Introduction of solar panel: Routing the configuration between solar cells with a switch matrix. However, their result models and control policies for the optimal output of solar panels. The smallest unit on a solar panel

  8. An Algorithm to Find Frequent Concepts of a Formal Context with Taxonomy

    E-Print Network [OSTI]

    Ferré, Sébastien

    An Algorithm to Find Frequent Concepts of a Formal Context with Taxonomy Peggy Cellier1 , S the data set is not structured. When an attribute taxonomy exists, existing techniques produce a com- pleted context with all attributes deduced from the taxonomy. Usual algorithms can then be applied

  9. Technology, Language, and Public Decisions: Finding Common Ground for Experts and Citizens

    E-Print Network [OSTI]

    Jensen, David

    Technology, Language, and Public Decisions: Finding Common Ground for Experts and Citizens David D@cs.umass.edu Todd M. La Porte School of Systems Engineering & Policy Analysis Delft University of Technology Delft, The Netherlands tlaporte@tmn.com Abstract---Many conflicts over specific technologies could be alleviated

  10. Finding Similar Documents in Document Collections Thorsten Brants and Reinhard Stolle

    E-Print Network [OSTI]

    Stolle, Reinhard

    and managing the collection. In partic­ ular, the document collections become unwieldy and ulti­ mately, the automated or computer­ assisted management of collections of natural language documents requires a fineFinding Similar Documents in Document Collections Thorsten Brants and Reinhard Stolle Palo Alto

  11. Finding Similar Documents in Document Collections Thorsten Brants and Reinhard Stolle

    E-Print Network [OSTI]

    Stolle, Reinhard

    and managing the collection. In partic- ular, the document collections become unwieldy and ulti- mately, the automated or computer- assisted management of collections of natural language documents requires a fineFinding Similar Documents in Document Collections Thorsten Brants and Reinhard Stolle Palo Alto

  12. Finding Salient Features for Personal Web Page Categories Marilyn R. Wulfekuhler and William F. Punch

    E-Print Network [OSTI]

    Wulfekuhler, Marilyn

    is successful in discovering word grouops which can be used to find similar information on the World Wide Web. 1 documents can be found on the World Wide Web. First, we examine techniques which will classify training Introduction The explosive growth of the Internet and the World Wide Web has changed the way we think about

  13. Towards Finding Relevant Information Graphics: Identifying the Independent and Dependent Axis from User-written Queries

    E-Print Network [OSTI]

    Carberry, Sandra

    Towards Finding Relevant Information Graphics: Identifying the Independent and Dependent Axis from of Computer and Information Science University of Delaware Newark, DE 19716 Abstract Information graphics (non-pictorial graphics such as bar charts and line graphs) contain a great deal of knowl- edge. Information retrieval

  14. New Study Finds Strong Carbon Pollution Standards Improve Air Quality, Environment, and Health

    E-Print Network [OSTI]

    Mohan, Chilukuri K.

    New Study Finds Strong Carbon Pollution Standards Improve Air Quality, Environment, and Health, Co- benefits of Carbon Standards: Air Pollution Changes under Different 111d Options for Existing-by-state changes in harmful air pollution, it is the first study to quantify and map the co-benefits of power plant

  15. Web Unit Mining Finding and Classifying Subgraphs of Centre for Advanced Information Systems

    E-Print Network [OSTI]

    Aixin, Sun

    Web Unit Mining ­ Finding and Classifying Subgraphs of Web Pages Aixin Sun Centre for Advanced Technological University Singapore, 639798 aseplim@ntu.edu.sg ABSTRACT In web classification, most researchers assume that the ob- jects to classify are individual web pages from one or more web sites. In practice

  16. UMASS ADOPT-A-BASS PROGRAM Find out how I spend my

    E-Print Network [OSTI]

    Schweik, Charles M.

    UMASS ADOPT-A-BASS PROGRAM Find out how I spend my summers... Adopt me! The striped bass, an important recreational and commercial fish along the Atlantic Coast, winters in the mid-Atlantic and migrates to New England to feed each spring. ACT NOW Send a check for $330 made out to "UMASS Adopt-A-Bass

  17. Solar Power Systems Find A Professional Solar Energy Installer For Any

    E-Print Network [OSTI]

    Lovley, Derek

    Solar Power Systems Find A Professional Solar Energy Installer For Any Type Of System www.CleanEnergyAuthority.com Install Solar Panels Enter Your Zip Code & Connect To Pre-Screened Solar Panel Installers www.ServiceMagic.com Biomass Pumps Reliable metering for apps from microflow to scale-up & pilot plant www.isco.com The Solar

  18. Transition state-finding strategies for use with the growing string method Anthony Goodrow,1

    E-Print Network [OSTI]

    Bell, Alexis T.

    Transition state-finding strategies for use with the growing string method Anthony Goodrow,1 Alexis Efficient identification of transition states is important for understanding reaction mechanisms. Most transition state search algorithms require long computational times and a good estimate of the transition

  19. Finding Narrow Passages with Probabilistic Roadmaps: The Small Step Retraction Method

    E-Print Network [OSTI]

    Pratt, Vaughan

    Finding Narrow Passages with Probabilistic Roadmaps: The Small Step Retraction Method Mitul Saha, USA {mitul, latombe}@cs.stanford.edu Abstract - The efficiency of Probabilistic Roadmap (PRM) planners fattening the robot's free space, constructing a roadmap in the fattened free space, and repairing colliding

  20. Finding Cancer-Related Gene Combinations Using a Molecular Evolutionary Algorithm

    E-Print Network [OSTI]

    Finding Cancer-Related Gene Combinations Using a Molecular Evolutionary Algorithm Chan-Hoon Parka it possible to investigate the molecular-level mechanism of cancer more efficiently. Computational methods to prostate cancer microarray data. The ex- perimental results show that the PLM classifiers perform better