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Sample records for find unedited transcripts

  1. QTR - unedited comments | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    - unedited comments QTR - unedited comments PDF icon Comments received in response to the DOE-QTR Request For Information More Documents & Publications Table of QTR comments in response to Federal Register RFI Table of QTR comments in response to Federal Register RFI QTR table of respondents

  2. Finding

    Office of Legacy Management (LM)

    Finding of No Significant Impact Ground Water Compliance at the Grand Junction UMTRA Project Site (Climax Uranium Millsite) September 1999 U.S. Department of Energy Grand Junction Office Grand Junction, Colorado Finding of No Significant Impact Environmental Assessment The U.S. Department of Energy (DOE) proposes a strategy to achieve ground water compliance at the Grand Junction, Colorado, LJMTRA project site, formerly known as the Climax Uranium Millsite. The proposed compliance strategy is no

  3. Spring 2006 ASA Meeting Disclaimer

    U.S. Energy Information Administration (EIA) Indexed Site

    6 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration In two adjacent files you may find unedited transcripts of EIA's spring 2006 meeting with the American Statistical Association Committee on Energy Statistics. Beginning with the fall 2003 meeting, EIA no longer edits these transcripts. Summaries of previous meetings may be found to the right of the Thursday and Friday transcripts. The public meeting took place April 6 and 7,

  4. Spring 2006 ASA Meeting Disclaimer

    U.S. Energy Information Administration (EIA) Indexed Site

    7 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration In two adjacent files you will find unedited transcripts of EIA's spring 2007 meeting with the American Statistical Association Committee on Energy Statistics. Beginning with the fall 2003 meeting, EIA no longer edits these transcripts. Summaries of previous meetings can be found to the right of the Thursday and Friday transcripts. The public meeting took place April 19 and

  5. Spring 2008 ASA Meeting Disclaimer

    U.S. Energy Information Administration (EIA) Indexed Site

    8 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration In two adjacent files you will find unedited transcripts of EIA's fall 2008 meeting with the American Statistical Association Committee on Energy Statistics. Beginning with the fall 2003 meeting, EIA no longer edits these transcripts. Summaries of previous meetings can be found to the right of the Thursday and Friday transcripts. The public meeting took place October 23 and

  6. Spring 2008 ASA Meeting Disclaimer

    U.S. Energy Information Administration (EIA) Indexed Site

    8 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration In two adjacent files you will find unedited transcripts of EIA's spring 2008 meeting with the American Statistical Association Committee on Energy Statistics. Beginning with the fall 2003 meeting, EIA no longer edits these transcripts. Summaries of previous meetings can be found to the right of the Thursday and Friday transcripts. The public meeting took place on April 9,

  7. Spring 2009 ASA Meeting Disclaimer

    U.S. Energy Information Administration (EIA) Indexed Site

    9 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration In two adjacent files you will find unedited transcripts of EIA's spring 2009 meeting with the American Statistical Association Committee on Energy Statistics. Beginning with the fall 2003 meeting, EIA no longer edits these transcripts. Summaries of previous meetings can be found to the right of the Thursday and Friday transcripts. The public meeting took place on April 2

  8. Untitled Document

    U.S. Energy Information Administration (EIA) Indexed Site

    5 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration In two adjacent files you may find unedited transcripts of EIA's spring 2005 meeting with the American Statistical Association Committee on Energy Statistics. Beginning with the fall 2003 meeting, EIA no longer edits these transcripts. Summaries of previous meetings may be found to the right of the Thursday and Friday transcripts, here at:

  9. Fall 2005 Meeting of the ASA

    U.S. Energy Information Administration (EIA) Indexed Site

    6 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration In two adjacent files you may find unedited transcripts of EIA's fall 2006 meeting with the American Statistical Association Committee on Energy Statistics. Beginning with the fall 2003 meeting, EIA no longer edits these transcripts. Summaries of previous meetings may be found to the right of the Thursday and Friday transcripts. The public meeting took place October 6 and 7,

  10. Fall 2007 ASA Meeting Disclaimer

    U.S. Energy Information Administration (EIA) Indexed Site

    7 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration In two adjacent files you will find unedited transcripts of EIA's fall 2007 meeting with the American Statistical Association Committee on Energy Statistics. Beginning with the fall 2003 meeting, EIA no longer edits these transcripts. Summaries of previous meetings can be found to the right of the Thursday and Friday transcripts. The public meeting took place October 18 and

  11. Find Standards

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Standards Find Standards1354608000000Find StandardsIn IHS, please free up a standard for another person: downloadprint the PDF, then Log Out.NoQuestion? 667-5809library@lanl.go...

  12. Find Journals

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    | Department of Energy Find Energy Incentives Quicker and Easier with DSIRE Open Data and Website Find Energy Incentives Quicker and Easier with DSIRE Open Data and Website March 3, 2015 - 4:16pm Addthis The updated Database of State Incentives for Renewables & Efficiency helps homeowners and businesses find incentive programs that can reduce or defray installation or purchase costs of technologies like photovoltaic systems. | Photo by Dennis Schroeder, National Renewable Energy

  13. Find Clear

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Find Clear Slide Title Duration Status myMail@adobe.com http:Adobe.com More Info>>

    Go No ...

  14. Find Clear

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    p align"left">

    Find Clear Slide Title Duration Status myMail@adobe.com http:Adobe.com More Info>>

  15. Find Books

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Books Find Books The Library Catalog is available to the public, though many eBooks are limited to LANL researchers. Question? 667-5809 Email Library Catalog: Search here for all...

  16. Find Reports

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Reports Find Reports Most of the reports sources below are publicly accessible. Question? 667-5809 Email Unclassified Los Alamos Reports Library Catalog - Print and electronic (1943-present) Los Alamos Authors (LANL-only access) - LA-, LA-CP, and LA-UR reports (1973-present) Los Alamos Research Online - Find published versions of Los Alamos research (2006-present) Classified reports - contact the SI-RMS Technical Report Collection for an appointment: phone icon 5-4168 / email icon

  17. Find Patents

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Patents Find Patents Some of these resources are LANL-only and will require Remote Access. Questions? 667-5809 Email US Patents Google Patents - search USPTO patents in a Google interface USPTO Database (United States Patent and Trademark Office) DOE Patents - only patents resulting from DOE-sponsored research Worldwide Patents Espacenet Patent Search (European Patent Office) Free Patents Online SciFinder information (from Chemical Abstracts Service) Ordering Patents Thomson Innovation - contact

  18. EIS-0458: Scoping Meeting Transcript

    Broader source: Energy.gov [DOE]

    Transcript of the public scoping meeting on the draft EIS for the proposed loan guarantee for the First Solar Topaz Solar Farm Project in San Luis Obispo County, CA.

  19. Transcript for Building America Video

    Broader source: Energy.gov [DOE]

    Transcript of a video about how DOE’s Building America program is helping to bridge the gap between homes with high energy costs and homes that are healthy, durable, and energy efficient.

  20. How to Find

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Find How to Find Some of these resources are LANL-only and will require Remote Access. Questions? 667-5809 Email Articles/Databases Search articles, books, proceedings, reports, etc. Los Alamos Research Online - Currently Los Alamos Research Online contains citations of publications from 2006 to present. Primo - search databases, publisher websites, Library collections, and more in one discovery tool Web of Science - comprehensive science database (includes alerts, citation analysis, etc.) More

  1. Find Conference Proceedings

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Conference Proceedings Find Conference Proceedings Many of these resources are LANL-only and will require Remote Access. Questions? 667-5809 Email Library Catalog search tips Conferences can be cited and published under different titles. Author/Title searches may not find a conference, even if we have it in print. Here is one strategy: Click on "Advanced Search" tab Choose "Words in all fields" field Enter keywords from the conference title and location Plutonium and

  2. Transcription factor-based biosensor

    DOE Patents [OSTI]

    2013-10-08

    The present invention provides for a system comprising a BmoR transcription factor, a .sigma..sup.54-RNA polymerase, and a pBMO promoter operatively linked to a reporter gene, wherein the pBMO promoter is capable of expression of the reporter gene with an activated form of the BmoR and the .sigma..sup.54-RNA polymerase.

  3. Find Theses and Dissertations

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Theses and Dissertations Find Theses and Dissertations Most of the theses sources below are publicly accessible. Questions? 667-5809 Email Electronic Databases Open Access Theses and Dissertations - Results limited to freely accessible, full-text ETDs Center for Research Libraries - Foreign Dissertations Collection Theses Canada - Canadian theses from Library and Archives Canada EThOS (Electronic Theses Online Service) - UK theses from the British Library Networked Digital Library of Theses and

  4. Webinar-Transcript-Repeat.pdf | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Webinar-Transcript-Repeat.pdf Webinar-Transcript-Repeat.pdf PDF icon Webinar-Transcript-Repeat.pdf More Documents & Publications Title Title Slide 1

  5. Public Meeting Transcript: Sustainable Design Standards for Federal...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Public Meeting Transcript: Sustainable Design Standards for Federal Buildings Public Meeting Transcript: Sustainable Design Standards for Federal Buildings This transcript is from ...

  6. Electricity Advisory Committee Meeting, May 20, 2008 (TRANSCRIPT...

    Office of Environmental Management (EM)

    (TRANSCRIPT) Electricity Advisory Committee Meeting, May 20, 2008 (TRANSCRIPT) Transcript of the May 20, 2008 Electricity Advisory Committee Meeting including a FERC presentation ...

  7. Electricity Advisory Committee Meeting, October 29, 2010: Transcript...

    Energy Savers [EERE]

    Transcript Electricity Advisory Committee Meeting, October 29, 2010: Transcript Transcript of the Electricity Advisory Committee Meeting held on October 29, 2010. PDF icon ...

  8. Engaging Financial Institution Partners Transcript.doc | Department...

    Office of Environmental Management (EM)

    Engaging Financial Institution Partners Transcript.doc Engaging Financial Institution Partners Transcript.doc Engaging Financial Institution Partners Transcript.doc Microsoft ...

  9. Financing Residential Energy Efficiency with Carbon Offsets Transcript...

    Energy Savers [EERE]

    Financing Residential Energy Efficiency with Carbon Offsets Transcript Financing Residential Energy Efficiency with Carbon Offsets Transcript This document contains the transcript...

  10. Using regulations.gov to find dockets and documents

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    regulations.gov to find dockets and documents Regulations.gov is a web-based repository of Federal regulatory documents. Publicly accessible documents associated with DOE Appliance and Equipment Standards rulemakings and related Federal Register notices are now accessible on regulations.gov On regulations.gov, you can: View Proposed and Final Rules and other regulatory documents or notices Read public comments, as well as public meeting presentations and transcripts These documents are part of a

  11. Finding of No Significant Impact

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    U.S. Department of Energy Finding of No Significant Impact 2 June 2001 This page intentionally left blank. U.S. Department of Energy Finding of No Significant Impact 12 June 2001 This page intentionally left blank.

  12. ARM - Research Highlights: Notable Findings

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    HighlightsNotable Research Findings Form Submit a New Research Highlight Sort Highlights Submitter Title Research Area Working Group Submission Date DOE Progress Reports Notable Research Findings for 2001-2006 Office of Science Abstracts Database Research Highlights Summaries Notable Research Findings for the Past Five Years Aerosols Cloud Parameterization and Modeling (Currently Cloud Modeling) Cloud Properties Instantaneous Radiative Flux (Currently Radiative Processes)

  13. transcript_pv_mapper.doc | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    transcript_pv_mapper.doc transcript_pv_mapper.doc transcript_pv_mapper.doc Microsoft Office document icon transcript_pv_mapper.doc More Documents & Publications PVMapper: A Tool for Energy Siting transcript_jedi_model.doc 2009 National Electric Transmission Congestion Study - San Francisco Workshop

  14. Find a Job | Careers | NREL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Interested in joining our team? Find your opportunity We're passionate about our mission-to develop clean energy and energy efficiency technologies and practices, advance...

  15. Electricity Advisory Committee Meeting, September 25, 2008: Transcript...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Transcript Electricity Advisory Committee Meeting, September 25, 2008: Transcript Transcript of the Electricity Advisory Committee Meeting 3:30 p.m. through 5:55 p.m on Thursday, ...

  16. TAP Webcast Transcript July-29, 2009

    Broader source: Energy.gov [DOE]

    Transcript of the audio for the July 2009 TAP Webcast titled Economic Impacts of Renewable Energy sponsored by the U.S. Department of Energy (DOE) Technical Assistance Project (TAP) for state and local officials.

  17. Engineering an allosteric transcription factor to respond to...

    Office of Scientific and Technical Information (OSTI)

    Engineering an allosteric transcription factor to respond to new ligands Citation Details In-Document Search Title: Engineering an allosteric transcription factor to respond to new ...

  18. Find Trainings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Clean Energy Jobs & Career Planning » Find Trainings Find Trainings To pursue a clean energy career, you may need general as well as specialized training. This page has links to training and courses in energy efficiency and renewable energy. This list is a work in progress and is not intended to be all-inclusive or to assure individual program quality. Many of these trainings are local, state or organizational based. Seeking a degree, certificate or education? Check out the Education &

  19. Transcription factor-based biosensors for detecting dicarboxylic acids

    DOE Patents [OSTI]

    Dietrich, Jeffrey; Keasling, Jay

    2014-02-18

    The invention provides methods and compositions for detecting dicarboxylic acids using a transcription factor biosensor.

  20. Department of Energy (DOE) Webinar Transcript | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    (DOE) Webinar Transcript Department of Energy (DOE) Webinar Transcript PDF icon Department of Energy (DOE) Webinar Transcript More Documents & Publications Welcome to the Department of Energy’s Recovery Act webinar on reporting in to federalreporting Department of Energy (DOE) Webinar Transcript Registration Overview for OMB Section 1512 Reporting for DOE Prime Recipients

  1. Peak finding using biorthogonal wavelets

    SciTech Connect (OSTI)

    Tan, C.Y.

    2000-02-01

    The authors show in this paper how they can find the peaks in the input data if the underlying signal is a sum of Lorentzians. In order to project the data into a space of Lorentzian like functions, they show explicitly the construction of scaling functions which look like Lorentzians. From this construction, they can calculate the biorthogonal filter coefficients for both the analysis and synthesis functions. They then compare their biorthogonal wavelets to the FBI (Federal Bureau of Investigations) wavelets when used for peak finding in noisy data. They will show that in this instance, their filters perform much better than the FBI wavelets.

  2. Writing audit findings: Be reasonable!

    SciTech Connect (OSTI)

    Girvin, N.W.

    1992-05-01

    A customary approach to auditing and reporting deficiencies is to keep a running list of those that are found, evaluate the severity of each, and based on the evidence, document findings or observations or concerns in an audit report. The report is issued and the auditee is normally requested to address ``root cause`` as part of their corrective action. This paper describes a ``root problems`` approach to documenting audit findings that is designed not only to put the QA auditor in a more favorable light, but to more effectively enable the auditee to identify root cause and meaningful corrective action. The positive results of this approach are considerable. You will have fewer findings but those you do have will be substantial. You will cite requirements that sound reasonable and make arguments difficult. If some of the supporting deficiencies (examples) prove to be incorrect, you will still have ample support for the original finding. You will be seen as reasonable individual who can help lead the auditee towards identification of root cause without taking away part of the responsibility. You even have a fair chance of fostering a sense of commitment to quality improvement on the auditee`s part. This in itself, is its own reward.

  3. Writing audit findings: Be reasonable

    SciTech Connect (OSTI)

    Girvin, N.W.

    1992-05-01

    A customary approach to auditing and reporting deficiencies is to keep a running list of those that are found, evaluate the severity of each, and based on the evidence, document findings or observations or concerns in an audit report. The report is issued and the auditee is normally requested to address root cause'' as part of their corrective action. This paper describes a root problems'' approach to documenting audit findings that is designed not only to put the QA auditor in a more favorable light, but to more effectively enable the auditee to identify root cause and meaningful corrective action. The positive results of this approach are considerable. You will have fewer findings but those you do have will be substantial. You will cite requirements that sound reasonable and make arguments difficult. If some of the supporting deficiencies (examples) prove to be incorrect, you will still have ample support for the original finding. You will be seen as reasonable individual who can help lead the auditee towards identification of root cause without taking away part of the responsibility. You even have a fair chance of fostering a sense of commitment to quality improvement on the auditee's part. This in itself, is its own reward.

  4. Find Jobs | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Jobs Find Jobs Clean energy jobs can be found in the public, private, and nonprofit sectors and can range from entry-level to professional positions in a wide range of fields. This section can help you familiarize yourself with different types of green energy jobs as you begin your search for a job that meets your interests and skills. EERE Career Opportunities EERE Career Opportunities Energy Efficency and Renewable Energy, the DOE office leading the nation's effort to enhance energy efficiency

  5. Transcriptional enhancer from milk protein genes

    DOE Patents [OSTI]

    Casperson, Gerald F.; Schmidhauser, Christian T.; Bissell, Mina J.

    1999-01-01

    The invention relates to novel enhancer nucleotide sequences which stimulate transcription of heterologous DNA in cells in culture. The enhancers are derived from major milk protein genes by the process of deletion mapping and functional analysis. The invention also relates to expression vectors containing the novel enhancers.

  6. HIV transcription is induced in dying cells

    SciTech Connect (OSTI)

    Woloschak, G.E.; Chang-Liu, Chin-Mei; Schreck, S. |; Panozzo, J.; Libertin, C.R.

    1996-02-01

    Using HeLa cells stably transfected with an HIV-LTR-CAT construct, we demonstrated a peak in CAT induction that occurs in viable (but not necessarily cell-division-competent) cells 24 h following exposure to some cell-killing agents. {gamma} rays were the only cell-killing agent which did not induce HIV transcription; this can be attributed to the fact that {gamma}-ray-induced apoptotic death requires functional p53, which is not present in HeLa cells. For all other agents, HIV-LTR induction was dose-dependent and correlated with the amount of cell killing that occurred in the culture. Doses which caused over 99% cell killing induced HIV-LTR transcription maximally, demonstrating that cells that will go on to die by 14 days are the cells expressing HIV-LTR-CAT.

  7. Blackout 2003: Transcript of Technical Conference with the Electric System

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Investigation Team | Department of Energy Transcript of Technical Conference with the Electric System Investigation Team Blackout 2003: Transcript of Technical Conference with the Electric System Investigation Team Electric System Investigation Team: 8 Reliability Recommendation consultation Transcript of the technical conference sponsored by the U.S. Canada task force investigating the August 14, 2003 blackout across Canada and the Northeastern United States. The transcript includes reports

  8. 20130226_sunshot_transcript_lbnl.doc | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    transcript_lbnl.doc 20130226_sunshot_transcript_lbnl.doc 20130226_sunshot_transcript_lbnl.doc Microsoft Office document icon 20130226_sunshot_transcript_lbnl.doc More Documents & Publications Why Are Resiential PV Prices in Germany So Much Lower Than in the United States? text_alternative_stat_webinar_2012_7_25.docx An Analysis of Residential PV System Price Differences between the United States and Germany

  9. Blackout 2003: Transcript of Technical Conference with the Electric...

    Broader source: Energy.gov (indexed) [DOE]

    Electric System Investigation Team: 8 Reliability Recommendation consultation Transcript ... discussions from the Electric System Investigation team on reliability recommendations. ...

  10. Financing Residential Energy Efficiency with Carbon Offsets Transcript |

    Energy Savers [EERE]

    Department of Energy Financing Residential Energy Efficiency with Carbon Offsets Transcript Financing Residential Energy Efficiency with Carbon Offsets Transcript This document contains the transcript for the Financing Residential Energy Efficiency with Carbon Offsets webinar held on March 12, 2013. PDF icon financing_residential_energy_efficiency_carbon_offsets_transcript.pdf More Documents & Publications Questions Asked during the Financing Residential Energy Efficiency with Carbon

  11. csep_transcript_transportation.doc | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    More Documents & Publications cseptranscriptminneapolis.doc Marketing & Driving Demand: Social Media Tools & Strategies - January 16, 2011 (Text Version) Transcript: Biomass ...

  12. CSC Workshop Transcript 02-20-2105 | Department of Energy

    Energy Savers [EERE]

    Workshop Transcript 02-20-2105 CSC Workshop Transcript 02-20-2105 PDF icon CSC Workshop Transcript 02-20-2105 More Documents & Publications EA-1971: FERC Notice of Intent to Prepare an Environmental Assessment EIS-0501: Notice of Intent Testimony

  13. Regional Transmission Projects: Finding Solutions

    SciTech Connect (OSTI)

    The Keystone Center

    2005-06-15

    The Keystone Center convened and facilitated a year-long Dialogue on "Regional Transmission Projects: Finding Solutions" to develop recommendations that will help address the difficult and contentious issues related to expansions of regional electric transmission systems that are needed for reliable and economic transmission of power within and across regions. This effort brought together a cross-section of affected stakeholders and thought leaders to address the problem with the collective wisdom of their experience and interests. Transmission owners sat at the table with consumer advocates and environmental organizations. Representatives from regional transmission organizations exchanged ideas with state and federal regulators. Generation developers explored common interests with public power suppliers. Together, the Dialogue participants developed consensus solutions about how to begin unraveling some of the more intractable issues surrounding identification of need, allocation of costs, and reaching consensus on siting issues that can frustrate the development of regional transmission infrastructure. The recommendations fall into three broad categories: 1. Recommendations on appropriate institutional arrangements and processes for achieving regional consensus on the need for new or expanded transmission infrastructure 2. Recommendations on the process for siting of transmission lines 3. Recommendations on the tools needed to support regional planning, cost allocation, and siting efforts. List of Dialogue participants: List of Dialogue Participants: American Electric Power American Transmission Company American Wind Energy Association California ISO Calpine Corporation Cinergy Edison Electric Institute Environmental Defense Federal Energy Regulatory Commission Great River Energy International Transmission Company ISO-New England Iowa Public Utility Board Kanner & Associates Midwest ISO National Association of Regulatory Utility Commissioners National Association of State Utility Consumer Advocates National Grid Northeast Utilities PA Office of Consumer Advocates Pacific Gas & Electric Corporation Pennsylvania Public Utility Commission PJM Interconnection The Electricity Consumers Resource Council U.S. Department of Energy US Department of the Interior Van Ness Feldman Western Interstate Energy Board Wind on the Wires Wisconsin Public Service Commission Xcel Energy

  14. Floodplain Statement of Findings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    EA-1796: Finding of No Significant Impact Floodplain Assessment and Statement of Findings Floodplain Assessment for Installation of a Renewable Energy Anaerobic Digester Facility

  15. Finding Hidden Oil and Gas Reserves

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Finding Hidden Oil and Gas Reserves Finding Hidden Oil and Gas Reserves Key Challenges: Seismic imaging methods, vital in our continuing search for deep offshore oil and gas...

  16. NREL Publishes Gearbox Reliability Collaborative Findings Report...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Publishes Gearbox Reliability Collaborative Findings Report NREL Publishes Gearbox Reliability Collaborative Findings Report October 3, 2011 - 12:56pm Addthis This is an excerpt ...

  17. Canfield's Couplet Clues for Finding Superconductors

    ScienceCinema (OSTI)

    Canfield, Paul

    2015-06-30

    Ames Laboratory scientist, Paul Canfield, summarizes his thoughts about finding new superconducting materials.

  18. Unlocking the Mysteries of the J. Robert Oppenheimer Transcript |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Unlocking the Mysteries of the J. Robert Oppenheimer Transcript Unlocking the Mysteries of the J. Robert Oppenheimer Transcript October 3, 2014 - 2:48pm Addthis This is an excerpt from the original press release from the Atomic Energy Commission announcing the publication of the redacted Oppenheimer transcript. | Photo courtesy of the Energy Department. This is an excerpt from the original press release from the Atomic Energy Commission announcing the publication of the

  19. Public Meeting Transcript: Sustainable Design Standards for Federal

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Buildings | Department of Energy Public Meeting Transcript: Sustainable Design Standards for Federal Buildings Public Meeting Transcript: Sustainable Design Standards for Federal Buildings This transcript is from the public meeting to discuss sustainable design standards for Federal buildings held on July 28, 2010. PDF icon nopr_meeting072810.pdf More Documents & Publications Public Roundtable- Data Access and Privacy Issues Related to Smart Grid Technologies Office of Information

  20. Reducing nontemplated 3' nucleotide addition to polynucleotide transcripts

    DOE Patents [OSTI]

    Kao, C. Cheng

    2000-01-01

    Non-template 3' nucleotide addition to a transcript is reduced by transcribing a transcript from a template comprising an ultimate and/or penultimate 5' ribose having a C'2 substituent such as methoxy, which reduces non-template 3' nucleotide addition to the transcript. The methods are shown to be applicable to a wide variety of polymerases, including Taq, T7 RNA polymerase, etc.

  1. HIV transcription is induced in dying cells

    SciTech Connect (OSTI)

    Woloschak, G.E.; Chang-Liu, Chin-Mei; Schreck, S. |

    1995-06-01

    Using HeLa cells stably transfected with an HIV-LTR-CAT construct, we demonstrated a peak in CAT induction that occurs in viable (but not necessarily cell-division-competent) cells 24 h following exposure to some cell-killing agents. {gamma} rays were the only cell-killing agent which did not induce HIV transcription; this can be attributed to the fact that {gamma}-ray-induced apoptotic death requires functional p53, which is not present in HeLa cells. For all other agents, HIV-LTR induction was dose-dependent and correlated with the amount of cell killing that occurred in the culture. 14 refs., 4 figs., 1 tab.

  2. Transcript of March 28, 2013, TAP webinar titled Internal Benchmarking...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    TAP webinar titled Internal Benchmarking Outreach and Data Collection Techniques Transcript of March 28, 2013, TAP webinar titled Internal Benchmarking Outreach and Data ...

  3. transcript_jedi_model.doc | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    document icon transcriptjedimodel.doc More Documents & Publications TAP Webcast Transcript July-29, 2009 Scenario Jedi Tools for Designing & Implementing Better Finance Programs

  4. Partnering With Utilities to Offer Energy Efficiency Programs Webinar Transcript

    Broader source: Energy.gov [DOE]

    Partnering With Utilities to Offer Energy Efficiency Programs Webinar Transcript, from the U.S. Department of Energy Technical Assistance Program (TAP).

  5. Transcript of May 4 Webinar: Understanding the Energy Policy...

    Office of Environmental Management (EM)

    Transcript of May 4 Webinar: Understanding the Energy Policy and Regulatory Environment ... energy initiatives to reduce greenhouse gas emissions, reduce and levelize retail ...

  6. TE Connectivity Finds Answers in Tomography

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    TE Connectivity Finds Answers in Tomography TE Connectivity Finds Answers in Tomography Print Thursday, 22 August 2013 10:50 TE Connectivity is a world leader in connectivity-the...

  7. Fossil Energy Word Find | Department of Energy

    Energy Savers [EERE]

    Word Find Fossil Energy Word Find Word Find (inlcuidng answer key) PDF icon Fossil Energy Word Search More Documents & Publications Fossil Energy Crossword Puzzle Coal Study Guide for Elementary School Guide to Low-Emission Boiler and Combustion Equipment Selection

  8. Fall 2015 EFRC Newsletter | U.S. DOE Office of Science (SC)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    5 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration In two adjacent files are the unedited transcripts of EIA's fall 2005 meeting with the American Statistical Association Committee on Energy Statistics. Beginning with the fall 2003 meeting, EIA no longer edits these transcripts. Summaries of previous meetings may be found to the right of the Thursday and Friday transcripts, here at:

  9. Fall 2005 Meeting of the ASA

    U.S. Energy Information Administration (EIA) Indexed Site

    5 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration In two adjacent files are the unedited transcripts of EIA's fall 2005 meeting with the American Statistical Association Committee on Energy Statistics. Beginning with the fall 2003 meeting, EIA no longer edits these transcripts. Summaries of previous meetings may be found to the right of the Thursday and Friday transcripts, here at:

  10. ORISE: Forensic scientist finds truth in fingerprints

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Forensic scientist finds truth in fingerprints For more than 30 years, Imogene Van Buren has searched for truth in the friction ridges of fingerprints. She has identified victims...

  11. Finding of No Significant Impact (FONSI)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    1 March 2002 FINDING OF NO SIGNIFICANT IMPACT TRANSURANIC WASTE RETRIEVAL FROM THE 218-W-4B AND 218-W-4C LOW-LEVEL BURIAL GROUNDS HANFORD SITE, RICHLAND, WASHINGTON U.S. DEPARTMENT OF ENERGY March 2002 U.S. Department of Energy Finding of No Significant Impact 2 March 2002 This page intentionally left blank. U.S. Department of Energy Finding of No Significant Impact 3 March 2002 AGENCY: U.S. Department of Energy ACTION: Finding of No Significant Impact SUMMARY: The U.S. Department of Energy

  12. Microsoft Word - Consolidated Verbatim Transcript CBS Meeting Sacramento California Transcripts_Final

    Office of Environmental Management (EM)

    Holiday Inn Capitol Plaza Sacramento 300 J Street Sacramento, CA 95814 April 26, 2016 VERBATIM TRANSCRIPT Mr. Jim Hamilton. Good afternoon. And for those in later time zones via webinar, good evening. Welcome, and thank you for being here today. My name is Jim Hamilton. I'm an advisor to the Department of Energy's Consent-Based Siting Team and my role here today is to help us all to have a good, open, productive conversation. To start off we have a few housekeeping issues I want to talk about

  13. Transcript, Keeping the Edge: Enhancing Performance by Managing Culture

    Broader source: Energy.gov [DOE]

    Transcript: 2003 INPO CEO Conference, Keeping the Edge: “Enhancing Performance by Managing Culture” Edgar H. Schein, Ph.D. Professor Emeritis, Massachusetts Institute of Technology November 7, 2003

  14. Roger D. Kornberg Polymerase, DNA, RNA, and Transcription

    Office of Scientific and Technical Information (OSTI)

    Roger D. Kornberg Polymerase, DNA, RNA, and Transcription Resources with Additional Information Roger D. Kornberg Credit: Linda A. Cicero/ Stanford News Service Roger D. Kornberg was awarded the 2006 Nobel Prize in Chemistry "for his studies of the molecular basis of eukaryotic transcription". He determined 'how DNA's genetic blueprint is read and used to direct the process for protein manufacture. Kornberg carried out a significant part of the research leading to this prize at the

  15. Method to determine transcriptional regulation pathways in organisms

    Office of Scientific and Technical Information (OSTI)

    (Patent) | SciTech Connect Method to determine transcriptional regulation pathways in organisms Citation Details In-Document Search Title: Method to determine transcriptional regulation pathways in organisms The invention relates to computer-implemented methods and systems for identifying regulatory relationships between expressed regulating polypeptides and targets of the regulatory activities of such regulating polypeptides. More specifically, the invention provides a new method for

  16. Method to determine transcriptional regulation pathways in organisms

    Office of Scientific and Technical Information (OSTI)

    (Patent) | SciTech Connect Method to determine transcriptional regulation pathways in organisms Citation Details In-Document Search Title: Method to determine transcriptional regulation pathways in organisms × You are accessing a document from the Department of Energy's (DOE) SciTech Connect. This site is a product of DOE's Office of Scientific and Technical Information (OSTI) and is provided as a public service. Visit OSTI to utilize additional information resources in energy science and

  17. Structural basis of JAZ repression of MYC transcription factors in

    Office of Scientific and Technical Information (OSTI)

    jasmonate signalling (Journal Article) | SciTech Connect Journal Article: Structural basis of JAZ repression of MYC transcription factors in jasmonate signalling Citation Details In-Document Search Title: Structural basis of JAZ repression of MYC transcription factors in jasmonate signalling Authors: Zhang, Feng ; Yao, Jian ; Ke, Jiyuan ; Zhang, Li ; Lam, Vinh Q. ; Xin, Xiu-Fang ; Zhou, X. Edward ; Chen, Jian ; Brunzelle, Joseph ; Griffin, Patrick R. ; Zhou, Mingguo ; Xu, H. Eric ; Melcher,

  18. Text Transcript of the Tribal Energy and Economic Development February

    Energy Savers [EERE]

    Webinar: Exploring Your Energy Markets | Department of Energy Text Transcript of the Tribal Energy and Economic Development February Webinar: Exploring Your Energy Markets Text Transcript of the Tribal Energy and Economic Development February Webinar: Exploring Your Energy Markets Randy Manion: Good morning or good afternoon wherever you may be and welcome to the second webinar of the 2016 DOE Tribal Renewable Energy Webinar Series: Exploring Your Energy Markets. I'm Randy Manion, today's

  19. RNA-guided transcriptional regulation (Patent) | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    Patent: RNA-guided transcriptional regulation Citation Details In-Document Search Title: RNA-guided transcriptional regulation Methods of modulating expression of a target nucleic acid in a cell are provided including introducing into the cell a first foreign nucleic acid encoding one or more RNAs complementary to DNA, wherein the DNA includes the target nucleic acid, introducing into the cell a second foreign nucleic acid encoding a nuclease-null Cas9 protein that binds to the DNA and is guided

  20. Portsmouth Integration Director's Final Findings and Order

    Broader source: Energy.gov [DOE]

    Portsmouth Integration Director's Final Findings and Order purpose is to: integrate the on-site work required for specific units to avoid duplication of effort, and efficiently perform sitewide...

  1. Missourian Finds New Opportunity in Energy Industry

    Broader source: Energy.gov [DOE]

    How one Missouri man decided to take matters into his own hands - creating a company that improves energy efficiency in homes and buildings - after not being able to find work during the recession.

  2. National Electric Transmission Congestion Study: Preliminary Findings

    Broader source: Energy.gov [DOE]

    The Department hosted three webinars in August 2012 to receive input and suggestions concerning the preliminary findings of the National Electric Transmission Congestion Study. The updated presentation used in the webinars is now available.

  3. NREL Publishes Gearbox Reliability Collaborative Findings Report |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Publishes Gearbox Reliability Collaborative Findings Report NREL Publishes Gearbox Reliability Collaborative Findings Report October 3, 2011 - 12:56pm Addthis This is an excerpt from the Third Quarter 2011 edition of the Wind Program R&D Newsletter. In June, DOE's National Renewable Energy Laboratory (NREL) published the first formal report on the efforts of the Gearbox Reliability Collaborative (GRC) to address a major challenge for the wind energy industry-gearbox

  4. Jefferson Laboratory Findings Excite Theoreticians, Experimentatlists |

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Jefferson Lab Laboratory Findings Excite Theoreticians, Experimentatlists Jefferson Laboratory Findings Excite Theoreticians, Experimentatlists March 1999 Seemingly reasonable assumptions define the human view of the world. But ruled of thumb can mislead - or be altogether incorrect. Fortunately, in physics, most assumptions can be tested. Those that don't measure up are amended. An experiment at the Department of Energy's Thomas Jefferson National Accelerator Facility (Jefferson Lab) has

  5. TE Connectivity Finds Answers in Tomography

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    TE Connectivity Finds Answers in Tomography TE Connectivity Finds Answers in Tomography Print Thursday, 22 August 2013 10:50 TE Connectivity is a world leader in connectivity-the $13 billion global company designs and manufactures more than 500,000 different electronic connectivity products for the automotive, energy, industrial, broadband communications, consumer device, healthcare, aerospace, and defense industries. TE Connectivity has a long-standing commitment to innovation and engineering

  6. Floodplain Assessment and Statement of Findings

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    and Statement of Findings for Installation of a Renewable Energy Anaerobic Digester Facility at the University of California, Davis in Yolo County, California January 28, 2013 1.0 Introduction This Floodplain Assessment and Statement of Findings has been prepared in accordance with 10 Code of Federal Regulations (CFR) 1022, "Compliance with Floodplain and Wetland Environmental Review Requirements" which were promulgated to implement the requirements of the U.S. Department of Energy's

  7. Mars Rover finds changing rocks, surprising scientists

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Mars Rover finds changing rocks, surprising scientists Mars Rover finds changing rocks, surprising scientists As NASA's Curiosity rover treks up a three-mile-high mountain on Mars, the rocks are changing. Back on Earth, scientists analyzing the data realized this was something different: It turned out to be the first of the high-silica rocks. December 24, 2015 Mars landscape This color-adjusted composite of images taken by NASA's Curiosity rover in September shows the lower portion of Mount

  8. Distributed PV Interconnection Recent Analysis Findings

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Recent Analysis Findings Page 1 of 18 Kristen Ardani, Miriam Makhyoun Page 1 of 18 [Speaker: Kristen Ardani] Cover Slide: Good afternoon, everyone. Thank you for joining the Distributed Generation Interconnection Collaborative informational webinar. Today, we are kicking off 2015 with a joint presentation from SEPA and NREL, in which each will discuss recent research and analysis findings related to interconnection. Slide 2: So really, the purpose of today's meeting is to hear recent research

  9. Distributed PV Interconnection: Recent Analysis Findings

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    January 21, 2015 "NREL and SEPA Recent Analysis Findings" Miriam Makhyoun, Solar Electric Power Association (SEPA) Kristen Ardani, National Renewable Energy Laboratory (NREL) 2 Purpose of Today's Meeting * Hear results from SEPA's recent survey of utility interconnection practices. o SEPA finds that utilities confront common challenges as they move towards more streamlined interconnection application processing. * Hear NREL results of forthcoming DGIC data collection and analysis,

  10. TE Connectivity Finds Answers in Tomography

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    TE Connectivity Finds Answers in Tomography TE Connectivity Finds Answers in Tomography Print Thursday, 22 August 2013 10:50 TE Connectivity is a world leader in connectivity-the $13 billion global company designs and manufactures more than 500,000 different electronic connectivity products for the automotive, energy, industrial, broadband communications, consumer device, healthcare, aerospace, and defense industries. TE Connectivity has a long-standing commitment to innovation and engineering

  11. AGENCY: ACTION: REVISED FINDING OF NO SIGNIFICANT IMPACT AND FLOODPLAIN STATEMENT OF FINDINGS

    Energy Savers [EERE]

    AGENCY: ACTION: REVISED FINDING OF NO SIGNIFICANT IMPACT AND FLOODPLAIN STATEMENT OF FINDINGS Gila to North Gila Transmission Line Rebuild and Upgrade Project Yuma County, Arizona DOE/EA-1948 U.S. Department of Energy, Western Area Power Administration Revised Finding of No Significant Impact and Floodplain Statement of Findings BACKGROUND: U.S. Department of Energy (DOE), Western Area Power Administration (Western) proposed to rebuild and upgrade the existing 4.8-mile-long Gila to North Gila

  12. Transcription Factors Bind Thousands of Active and InactiveRegions in the Drosophila Blastoderm

    SciTech Connect (OSTI)

    Li, Xiao-Yong; MacArthur, Stewart; Bourgon, Richard; Nix, David; Pollard, Daniel A.; Iyer, Venky N.; Hechmer, Aaron; Simirenko, Lisa; Stapleton, Mark; Luengo Hendriks, Cris L.; Chu, Hou Cheng; Ogawa, Nobuo; Inwood, William; Sementchenko, Victor; Beaton, Amy; Weiszmann, Richard; Celniker, Susan E.; Knowles, David W.; Gingeras, Tom; Speed, Terence P.; Eisen, Michael B.; Biggin, Mark D.

    2008-01-10

    Identifying the genomic regions bound by sequence-specific regulatory factors is central both to deciphering the complex DNA cis-regulatory code that controls transcription in metazoans and to determining the range of genes that shape animal morphogenesis. Here, we use whole-genome tiling arrays to map sequences bound in Drosophila melanogaster embryos by the six maternal and gap transcription factors that initiate anterior-posterior patterning. We find that these sequence-specific DNA binding proteins bind with quantitatively different specificities to highly overlapping sets of several thousand genomic regions in blastoderm embryos. Specific high- and moderate-affinity in vitro recognition sequences for each factor are enriched in bound regions. This enrichment, however, is not sufficient to explain the pattern of binding in vivo and varies in a context-dependent manner, demonstrating that higher-order rules must govern targeting of transcription factors. The more highly bound regions include all of the over forty well-characterized enhancers known to respond to these factors as well as several hundred putative new cis-regulatory modules clustered near developmental regulators and other genes with patterned expression at this stage of embryogenesis. The new targets include most of the microRNAs (miRNAs) transcribed in the blastoderm, as well as all major zygotically transcribed dorsal-ventral patterning genes, whose expression we show to be quantitatively modulated by anterior-posterior factors. In addition to these highly bound regions, there are several thousand regions that are reproducibly bound at lower levels. However, these poorly bound regions are, collectively, far more distant from genes transcribed in the blastoderm than highly bound regions; are preferentially found in protein-coding sequences; and are less conserved than highly bound regions. Together these observations suggest that many of these poorly-bound regions are not involved in early-embryonic transcriptional regulation, and a significant proportion may be nonfunctional. Surprisingly, for five of the six factors, their recognition sites are not unambiguously more constrained evolutionarily than the immediate flanking DNA, even in more highly bound and presumably functional regions, indicating that comparative DNA sequence analysis is limited in its ability to identify functional transcription factor targets.

  13. Electricity Advisory Committee Meeting, January 24, 2014- Meeting Summary and Transcript

    Broader source: Energy.gov [DOE]

    Meeting summary and transcript for the January 24, 2014 meeting of the Electricity Advisory Committee.

  14. Transcript of the Guidelines for Home Energy Professionals project Prezi presentation.

    Broader source: Energy.gov [DOE]

    This document contains the transcript of the Guidelines for Home Energy Professionals project Prezi presentation.

  15. Electricity Advisory Committee Meeting, March 10, 2011- Meeting Minutes and Transcript

    Broader source: Energy.gov [DOE]

    Meeting minutes and transcript for the March 10, 2011 meeting of the Electricity Advisory Committee.

  16. Community Development Finance Institutions-Opportunities for Partnerships with Energy Efficiency Programs Transcript.doc

    Broader source: Energy.gov [DOE]

    Community Development Finance Institutions-Opportunities for Partnerships with Energy Efficiency Programs Transcript.doc

  17. Energy Savings Performance Contracting-Savings Measurement and Verification Transcript 2-24-2011.doc

    Broader source: Energy.gov [DOE]

    Energy Savings Performance Contracting-Savings Measurement and Verification Transcript 2-24-2011.doc

  18. Resources for Handling Transcripts | U.S. DOE Office of Science (SC)

    Office of Science (SC) Website

    Graduate Transcripts for Current Graduate Institution » Resources for Handling Transcripts DOE Office of Science Graduate Student Research (SCGSR) Program SCGSR Home Eligibility Benefits Participant Obligations How to Apply Identifying a Collaborating DOE Laboratory Scientist Research Proposal Guidelines Office of Science Priority Research Areas for SCGSR Program Letters of Support Graduate Transcripts for Current Graduate Institution Resources for Handling Transcripts Application Evaluation

  19. Finding a Career in Energy Efficiency

    Broader source: Energy.gov [DOE]

    Kendra went to a county agency hoping they could help her find work, but what she really wanted was a career. That’s when she was referred to an upcoming weatherization training program hosted by the Laborers’ International Union of North America, a half-million strong labor union for construction workers, including weatherization workers.

  20. finding environmentally friendly materials for MHK devices

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    finding environmentally friendly materials for MHK devices - Sandia Energy Energy Search Icon Sandia Home Locations Contact Us Employee Locator Energy & Climate Secure & Sustainable Energy Future Stationary Power Energy Conversion Efficiency Solar Energy Wind Energy Water Power Supercritical CO2 Geothermal Natural Gas Safety, Security & Resilience of the Energy Infrastructure Energy Storage Nuclear Power & Engineering Grid Modernization Battery Testing Nuclear Fuel Cycle Defense

  1. Lab supercomputer finds new home at UNM

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Lab supercomputer to UNM Community Connections: Your link to news and opportunities from Los Alamos National Laboratory Latest Issue:May 2016 all issues All Issues » submit Lab supercomputer finds new home at UNM Chance of a lifetime for students with complex research projects March 1, 2015 UNM logo. UNM logo. Contact Community Programs Director Kurt Steinhaus Email Editor Ute Haker Email The New Mexico Consortium has donated Los Alamos National Laboratory's Ulam supercomputer to the University

  2. Occupancy by key transcription factors is a more accurate predictor of enhancer activity than histone modifications or chromatin accessibility

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Dogan, Nergiz; Wu, Weisheng; Morrissey, Christapher S.; Chen, Kuan-Bei; Stonestrom, Aaron; Long, Maria; Keller, Cheryl A.; Cheng, Yong; Jain, Deepti; Visel, Axel; et al

    2015-04-23

    Regulated gene expression controls organismal development, and variation in regulatory patterns has been implicated in complex traits. Thus accurate prediction of enhancers is important for further understanding of these processes. Genome-wide measurement of epigenetic features, such as histone modifications and occupancy by transcription factors, is improving enhancer predictions, but the contribution of these features to prediction accuracy is not known. Given the importance of the hematopoietic transcription factor TAL1 for erythroid gene activation, we predicted candidate enhancers based on genomic occupancy by TAL1 and measured their activity. Contributions of multiple features to enhancer prediction were evaluated based on the resultsmore » of these and other studies. Results: TAL1-bound DNA segments were active enhancers at a high rate both in transient transfections of cultured cells (39 of 79, or 56%) and transgenic mice (43 of 66, or 65%). The level of binding signal for TAL1 or GATA1 did not help distinguish TAL1-bound DNA segments as active versus inactive enhancers, nor did the density of regulation-related histone modifications. A meta-analysis of results from this and other studies (273 tested predicted enhancers) showed that the presence of TAL1, GATA1, EP300, SMAD1, H3K4 methylation, H3K27ac, and CAGE tags at DNase hypersensitive sites gave the most accurate predictors of enhancer activity, with a success rate over 80% and a median threefold increase in activity. Chromatin accessibility assays and the histone modifications H3K4me1 and H3K27ac were sensitive for finding enhancers, but they have high false positive rates unless transcription factor occupancy is also included. Conclusions: Occupancy by key transcription factors such as TAL1, GATA1, SMAD1, and EP300, along with evidence of transcription, improves the accuracy of enhancer predictions based on epigenetic features.« less

  3. Occupancy by key transcription factors is a more accurate predictor of enhancer activity than histone modifications or chromatin accessibility

    SciTech Connect (OSTI)

    Dogan, Nergiz; Wu, Weisheng; Morrissey, Christapher S.; Chen, Kuan-Bei; Stonestrom, Aaron; Long, Maria; Keller, Cheryl A.; Cheng, Yong; Jain, Deepti; Visel, Axel; Pennacchio, Len A.; Weiss, Mitchell J.; Blobel, Gerd A.; Hardison, Ross C.

    2015-04-23

    Regulated gene expression controls organismal development, and variation in regulatory patterns has been implicated in complex traits. Thus accurate prediction of enhancers is important for further understanding of these processes. Genome-wide measurement of epigenetic features, such as histone modifications and occupancy by transcription factors, is improving enhancer predictions, but the contribution of these features to prediction accuracy is not known. Given the importance of the hematopoietic transcription factor TAL1 for erythroid gene activation, we predicted candidate enhancers based on genomic occupancy by TAL1 and measured their activity. Contributions of multiple features to enhancer prediction were evaluated based on the results of these and other studies. Results: TAL1-bound DNA segments were active enhancers at a high rate both in transient transfections of cultured cells (39 of 79, or 56%) and transgenic mice (43 of 66, or 65%). The level of binding signal for TAL1 or GATA1 did not help distinguish TAL1-bound DNA segments as active versus inactive enhancers, nor did the density of regulation-related histone modifications. A meta-analysis of results from this and other studies (273 tested predicted enhancers) showed that the presence of TAL1, GATA1, EP300, SMAD1, H3K4 methylation, H3K27ac, and CAGE tags at DNase hypersensitive sites gave the most accurate predictors of enhancer activity, with a success rate over 80% and a median threefold increase in activity. Chromatin accessibility assays and the histone modifications H3K4me1 and H3K27ac were sensitive for finding enhancers, but they have high false positive rates unless transcription factor occupancy is also included. Conclusions: Occupancy by key transcription factors such as TAL1, GATA1, SMAD1, and EP300, along with evidence of transcription, improves the accuracy of enhancer predictions based on epigenetic features.

  4. Best Possible Strategy for Finding Ground States

    SciTech Connect (OSTI)

    Franz, Astrid; Hoffmann, Karl Heinz; Salamon, Peter

    2001-06-04

    Finding the ground state of a system with a complex energy landscape is important for many physical problems including protein folding, spin glasses, chemical clusters, and neural networks. Such problems are usually solved by heuristic search methods whose efficacy is judged by empirical performance on selected examples. We present a proof that, within the large class of algorithms that simulate a random walk on the landscape, threshold accepting is the best possible strategy. In particular, it can perform better than simulated annealing and Tsallis statistics. Our proof is the first example of a provably optimal strategy in this area.

  5. Find R&D Results

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Find R&D Results Your connection to science, technology, and engineering research information from the U.S. Department of Energy SciTech Connect contains: * energy-related R&D results from the 1940's to today; * 380,000 full-text DOE-sponsored scientific and technical information reports; * 2.6 million citations; * 1.4 million journal article citations, with 228,000 digital object identifiers; and * all the publicly accessible full-text journal articles and accepted manuscripts in DOE

  6. Trigger loop folding determines transcription rate of Escherichia colis RNA polymerase

    SciTech Connect (OSTI)

    Mejia, Yara X.; Nudler, Evgeny; Bustamante, Carlos

    2014-12-31

    Two components of the RNA polymerase (RNAP) catalytic center, the bridge helix and the trigger loop (TL), have been linked with changes in elongation rate and pausing. Here, single molecule experiments with the WT and two TL-tip mutants of the Escherichia coli enzyme reveal that tip mutations modulate RNAPs pause-free velocity, identifying TL conformational changes as one of two rate-determining steps in elongation. Consistent with this observation, we find a direct correlation between helix propensity of the modified amino acid and pause-free velocity. Moreover, nucleotide analogs affect transcription rate, suggesting that their binding energy also influences TL folding. A kinetic model in which elongation occurs in two steps, TL folding on nucleoside triphosphate (NTP) binding followed by NTP incorporation/pyrophosphate release, quantitatively accounts for these results. The TL plays no role in pause recovery remaining unfolded during a pause. The model suggests a finely tuned mechanism that balances transcription speed and fidelity.

  7. Trigger loop folding determines transcription rate of Escherichia coli’s RNA polymerase

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Mejia, Yara X.; Nudler, Evgeny; Bustamante, Carlos

    2014-12-31

    Two components of the RNA polymerase (RNAP) catalytic center, the bridge helix and the trigger loop (TL), have been linked with changes in elongation rate and pausing. Here, single molecule experiments with the WT and two TL-tip mutants of the Escherichia coli enzyme reveal that tip mutations modulate RNAP’s pause-free velocity, identifying TL conformational changes as one of two rate-determining steps in elongation. Consistent with this observation, we find a direct correlation between helix propensity of the modified amino acid and pause-free velocity. Moreover, nucleotide analogs affect transcription rate, suggesting that their binding energy also influences TL folding. A kineticmore » model in which elongation occurs in two steps, TL folding on nucleoside triphosphate (NTP) binding followed by NTP incorporation/pyrophosphate release, quantitatively accounts for these results. The TL plays no role in pause recovery remaining unfolded during a pause. The model suggests a finely tuned mechanism that balances transcription speed and fidelity.« less

  8. Focus Series: Program Finds Community "Readiness" Is the Key...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Program Finds Community "Readiness" Is the Key to More Retrofits Focus Series: Program Finds Community "Readiness" Is the Key to More Retrofits Focus Series: Program Finds ...

  9. UDAC 2014 Findings and Recommendations | Department of Energy

    Energy Savers [EERE]

    2014 Findings and Recommendations UDAC 2014 Findings and Recommendations PDF icon UDAC 2014 Findings and Recommendations More Documents & Publications Minutes from December 16, 2013 UDAC Meeting UDAC 2014 Report UDAC Findings and Recommendations 2014

  10. UDAC Findings and Recommendations 2014 | Department of Energy

    Energy Savers [EERE]

    Findings and Recommendations 2014 UDAC Findings and Recommendations 2014 PDF icon UDAC Findings and Recommendations 2014 More Documents & Publications Minutes from December 16, 2013 UDAC Meeting UDAC 2014 Report UDAC 2014 Findings and Recommendations

  11. Scientists Find Surprising Link Between Rain and Airborne Particles

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Scientists Find Surprising Link Between Rain and Airborne Particles

  12. Landfill aeration worldwide: Concepts, indications and findings

    SciTech Connect (OSTI)

    Ritzkowski, M.; Stegmann, R.

    2012-07-15

    Highlights: Black-Right-Pointing-Pointer Different landfill aeration concepts and accordant application areas are described. Black-Right-Pointing-Pointer Examples of full scale projects are provided for Europe, North-America and Asia. Black-Right-Pointing-Pointer Major project findings are summarised, including prospects and limitations. Black-Right-Pointing-Pointer Inconsistencies between laboratory and full scale results have been elaborated. Black-Right-Pointing-Pointer An explanatory approach in connection with the inconsistencies is provided. - Abstract: The creation of sustainable landfills is a fundamental goal in waste management worldwide. In this connection landfill aeration contributes towards an accelerated, controlled and sustainable conversion of conventional anaerobic landfills into a biological stabilized state associated with a minimised emission potential. The technology has been successfully applied to landfills in Europe, North America and Asia, following different strategies depending on the geographical region, the specific legislation and the available financial resources. Furthermore, methodologies for the incorporation of landfill aeration into the carbon trade mechanisms have been developed in recent years. This manuscript gives an overview on existing concepts for landfill aeration; their application ranges and specifications. For all of the described concepts examples from different countries worldwide are provided, including details regarding their potentials and limitations. Some of the most important findings from these aeration projects are summarised and future research needs have been identified. It becomes apparent that there is a great demand for a systematisation of the available results and implications in order to further develop and optimise this very promising technology. The IWWG (International Waste Working Group) Task Group 'Landfill Aeration' contributes towards the achievement of this goal.

  13. OSTI News Transcripts, International Group Gathers in Knoxville to Discuss

    Office of Scientific and Technical Information (OSTI)

    Sharing Science across Borders, ETDE, Office of Scientific and Technical Information, U.S. Department of Energy, www.osti.gov | OSTI, US Dept of Energy, Office of Scientific and Technical Information OSTI News Transcripts, International Group Gathers in Knoxville to Discuss Sharing Science across Borders, ETDE, Office of Scientific and Technical Information, U.S. Department of Energy, www.osti.gov International Group Gathers in Knoxville to Discuss Sharing Science across Borders (October

  14. Transcript: OSTI Highlights First-Time Electronic Availability of

    Office of Scientific and Technical Information (OSTI)

    Geothermal Documents at San Diego Geothermal Conference, Office of Scientific and Technical Information, U.S. Department of Energy, www.osti.gov | OSTI, US Dept of Energy, Office of Scientific and Technical Information Transcript: OSTI Highlights First-Time Electronic Availability of Geothermal Documents at San Diego Geothermal Conference, Office of Scientific and Technical Information, U.S. Department of Energy, www.osti.gov September 2006 Geothermal Subject Portal Listen Now Now, more than

  15. Transcription factors for modification of lignin content in plants

    DOE Patents [OSTI]

    Wang, Huanzhong; Chen, Fang; Dixon, Richard A.

    2015-06-02

    The invention provides methods for modifying lignin, cellulose, xylan, and hemicellulose content in plants, and for achieving ectopic lignification and, for instance, secondary cell wall synthesis in pith cells, by altered regulation of a WRKY transcription factor. Nucleic acid constructs for altered WRKY-TF expression are described. Transgenic plants are provided that comprise modified pith cell walls, and lignin, cellulose, and hemicellulose content. Plants described herein may be used, for example, as improved biofuel feedstock and as highly digestible forage crops.

  16. Transcript of Tribal Energy and Economic Webinar: Energy Planning for Tribal Economic Development

    Broader source: Energy.gov [DOE]

    Transcript for the Tribal Energy and Economic Webinar: Energy Planning for Tribal Economic Development held on Jan. 27, 2016.

  17. Electricity Advisory Committee Meeting, October 15-16, 2012- Meeting Summaries and Transcripts

    Broader source: Energy.gov [DOE]

    Meeting summaries and transcripts for the October 15-16, 2012 meeting of the Electricity Advisory Committee.

  18. Electricity Advisory Committee Meeting, June 11-12, 2012- Meeting Minutes and Transcripts

    Broader source: Energy.gov [DOE]

    Meeting minutes and transcripts for the June 11-12, 2012 meeting of the Electricity Advisory Committee.

  19. Electricity Advisory Committee Meeting, March 12-13, 2014- Meeting Minutes and Transcripts

    Broader source: Energy.gov [DOE]

    Meeting minutes and transcripts for the March 12-13, 2014 meeting of the Electricity Advisory Committee.

  20. Electricity Advisory Committee Meeting, March 5-6, 2012- Meeting Minutes and Transcripts

    Broader source: Energy.gov [DOE]

    Meeting minutes and transcripts for the March 5-6, 2012 meeting of the Electricity Advisory Committee.

  1. Electricity Advisory Committee Meeting, June 16-17, 2014- Meeting Summaries and Transcripts

    Broader source: Energy.gov [DOE]

    Meeting summaries and transcripts for the June 16-17, 2014 meeting of the Electricity Advisory Committee.

  2. Electricity Advisory Committee Meeting, September 24-25, 2014- Meeting Summaries and Transcripts

    Broader source: Energy.gov [DOE]

    Meeting summaries and transcripts for the September 24-25, 2014 meeting of the Electricity Advisory Committee.

  3. Meeting of the Electricity Advisory Committee Meeting, September 29-30, 2015- Meeting Minutes and Transcripts

    Broader source: Energy.gov [DOE]

    Meeting minutes and transcripts for the September 29-30, 2015 meeting of the Electric Advisory Committee.

  4. Transcriptional control in Alicyclobacillus acidocaldarius and associated genes, proteins, and methods

    DOE Patents [OSTI]

    Lee, Brady Deneys; Thompson, David N; Apel, William A.; Thompson, Vicki Slavchev; Reed, David W; Lacey, Jeffrey A

    2014-05-06

    Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods of modulating transcription or transcription or transcriptional control using isolated and/or purified polypeptides and nucleic acid sequences from Alicyclobacillus acidocaldarius.

  5. Transcriptional control in Alicyclobacillus acidocaldarius and associated genes, proteins, and methods

    DOE Patents [OSTI]

    Lee, Brady D.; Thompson, David N.; Apel, William A.; Thompson, Vicki S.; Reed, David W.; Lacey, Jeffrey A.

    2015-11-17

    Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods of modulating transcription or transcription or transcriptional control using isolated and/or purified polypeptides and nucleic acid sequences from Alicyclobacillus acidocaldarius.

  6. The transcriptional diversity of 25 Drosophila cell lines

    SciTech Connect (OSTI)

    Cherbas, Lucy; Willingham, Aarron; Zhang, Dayu; Yang, Li; Zou, Yi; Eads, Brian D.; Carlson, Joseph W.; Landolin, Jane M.; Kapranov, Philipp; Dumais, Jacqueline; Samsonova, Anastasia; Choi, Jeong-Hyeon; Roberts, Johnny; Davis, Carrie A.; Tang, Haixu; van Baren, Marijke J.; Ghosh, Srinka; Dobin, Alexander; Bell, Kim; Lin, Wei; Langton, Laura; Duff, Michael O.; Tenney, Aaron E.; Zaleski, Chris; Brent, Michael R.; Hoskins, Roger A.; Kaufman, Thomas C.; Andrews, Justen; Graveley, Brenton R.; Perrimon, Norbert; Celniker, Susan E.; Gingeras, Thomas R.; Cherbas, Peter

    2010-11-15

    Drosophila melanogaster cell lines are important resources for cell biologists. Here, we catalog the expression of exons, genes, and unannotated transcriptional signals for 25 lines. Unannotated transcription is substantial (typically 19% of euchromatic signal). Conservatively, we identify 1405 novel transcribed regions; 684 of these appear to be new exons of neighboring, often distant, genes. Sixty-four percent of genes are expressed detectably in at least one line, but only 21% are detected in all lines. Each cell line expresses, on average, 5885 genes, including a common set of 3109. Expression levels vary over several orders of magnitude. Major signaling pathways are well represented: most differentiation pathways are off and survival/growth pathways on. Roughly 50% of the genes expressed by each line are not part of the common set, and these show considerable individuality. Thirty-one percent are expressed at a higher level in at least one cell line than in any single developmental stage, suggesting that each line is enriched for genes characteristic of small sets of cells. Most remarkable is that imaginal discderived lines can generally be assigned, on the basis of expression, to small territories within developing discs. These mappings reveal unexpected stability of even fine-grained spatial determination. No two cell lines show identical transcription factor expression. We conclude that each line has retained features of an individual founder cell superimposed on a common cell line gene expression pattern. Wereport the transcriptional profiles of 25 Drosophila melanogaster cell lines, principally by whole-genome tiling microarray analysis of total RNA, carried out as part of the modENCODE project. The data produced in this study add to our knowledge of the cell lines and of the Drosophila transcriptome in several ways. We summarize the expression of previously annotated genes in each of the 25 lines with emphasis on what those patterns reveal about the origins of the lines and the stability of spatial expression patterns. We also offer an initial analysis of previously unannotated transcripts in the cell lines.

  7. EA-1922: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1922: Finding of No Significant Impact Combined Power and Biomass Heating System, Fort Yukon, Alaska Based on the EA, DOE finds that providng ...

  8. EA-1965: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    5: Finding of No Significant Impact EA-1965: Finding of No Significant Impact ... Based on the environmental assessment (EA), DOE finds that the proposed action is not a major ...

  9. EA-1733: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1733: Finding of No Significant Impact Calpine Enhanced Geothermal Systems Project, Sonoma County, California DOE determined that providing...

  10. EA-1839: Final Environmental Assessment and Finding of No Significant...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    39: Final Environmental Assessment and Finding of No Significant Impact EA-1839: Final Environmental Assessment and Finding of No Significant Impact Department of Energy Loan ...

  11. EA-1984: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1984: Finding of No Significant Impact Disposition of Five Signature Properties at Idaho National Laboratory DOE has decided to implement the...

  12. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact Silver Peak Area Geothermal Exploration Project, Esmeralda County, NV December 7, 2012 EA-1863: Finding of No Significant Impact Vegetation...

  13. Innovative Idaho Site Crews Find Ways to Make Waste Retrieval...

    Office of Environmental Management (EM)

    Innovative Idaho Site Crews Find Ways to Make Waste Retrieval Safer, More Efficient Innovative Idaho Site Crews Find Ways to Make Waste Retrieval Safer, More Efficient June 30, ...

  14. EA-1378: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    378: Finding of No Significant Impact EA-1378: Finding of No Significant Impact Site-Wide Environmental Assessment of National Renewable Energy Laboratory's National Wind ...

  15. EA-1535: Finding of No Significant Impact | Department of Energy

    Energy Savers [EERE]

    Finding of No Significant Impact EA-1535: Finding of No Significant Impact Uranium Leasing Program U.S. Department of Energy Office of Legacy Management prepared the Uranium...

  16. EA-1829: Final Environmental Assessment and Finding of No Significant...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Final Environmental Assessment and Finding of No Significant Impact EA-1829: Final Environmental Assessment and Finding of No Significant Impact Phycal Algae Pilot Project, Wahiawa...

  17. EA-1866: Final Environmental Assessment and Finding of No Significant...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    6: Final Environmental Assessment and Finding of No Significant Impact EA-1866: Final Environmental Assessment and Finding of No Significant Impact Argonne National Laboratory...

  18. EA-1889: Final Environmental Assessment and Finding of No Significant...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Final Environmental Assessment and Finding of No Significant Impact EA-1889: Final Environmental Assessment and Finding of No Significant Impact Disposal of Decommissioned,...

  19. EA-1869: Final Environmental Assessment and Finding of No Significant...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    69: Final Environmental Assessment and Finding of No Significant Impact EA-1869: Final Environmental Assessment and Finding of No Significant Impact General Motors LLC, Electric...

  20. EA-1769: Final Environmental Assessment and Finding of No Significant...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    9: Final Environmental Assessment and Finding of No Significant Impact EA-1769: Final Environmental Assessment and Finding of No Significant Impact Houston Advanced Research...

  1. Innovative Financing Solutions: Finding Money for Your Energy...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Innovative Financing Solutions: Finding Money for Your Energy Efficiency Projects Innovative Financing Solutions: Finding Money for Your Energy Efficiency Projects Provides an ...

  2. EA-1745: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1745: Finding of No Significant Impact Blast ... Based on the analyses in the environmental assessment, DOE determined that its proposed action - ...

  3. EA-1091: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    EA-1091-FONSI-1996.pdf More Documents & Publications EA-1091: Final Environmental Assessment EA-1157: Finding of No Significant Impact EA-1183: Finding of No Significant Impact

  4. EA-2022: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    PDF icon EA-2022: Finding of No Significant Impact More Documents & Publications EA-1602: Finding of No Significant Impact EA-2022: Final Environmental Assessment EA-2022: Draft ...

  5. Business Case for Small Modular Reactors Report on Findings ...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Business Case for Small Modular Reactors Report on Findings Business Case for Small Modular Reactors Report on Findings This study assesses the market for SMRs and develops a ...

  6. EA-1747: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    7: Finding of No Significant Impact EA-1747: Finding of No Significant Impact Rocky Flats Surface Water Configuration, Jefferson County, Colorado Surface Water Configuration...

  7. EA-1853: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of No Significant Impact EA-1853: Finding of No Significant Impact Perrin Ranch Wind Energy Interconnection Project, Coconino County, Arizona Based on findings and analysis in...

  8. EA-1785: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    5: Finding of No Significant Impact EA-1785: Finding of No Significant Impact Phase III Anthropogenic Test Characterization Project, Mobile County, Alabama Based on the analyses in...

  9. EA-1938: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1938: Finding of No Significant Impact Grieve Unit CO2 Enhanced Recovery Project, Natrona County, WY Western has reviewed BLM's EA "Grieve...

  10. EA-1784: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    4: Finding of No Significant Impact EA-1784: Finding of No Significant Impact Fotowatio Nevada Solar, LLC's APEX Solar Power Project in Clark County, Nevada The Department of...

  11. EA-1862: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1862: Finding of No Significant Impact Oneida Seven Generations Corporation: Energy Recovery Project, Green Bay, Wisconsin The U.S. Department...

  12. EA-1832: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    32: Finding of No Significant Impact EA-1832: Finding of No Significant Impact Rainer Biogas LLC Community Anaerobic Manure Digester, Enumclaw, King County, Washington The U.S....

  13. Jefferson Lab finds its man Mont (Inside Business) | Jefferson...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    https:www.jlab.orgnewsarticlesjefferson-lab-finds-its-man-mont-inside-business Jefferson Lab finds its man Mont Hugh Montgomery Hugh Montgomery, a British nuclear physicist...

  14. EA-1802: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    2: Finding of No Significant Impact EA-1802: Finding of No Significant Impact Chicago View Wind Project Chicago Heights, Cook County, Illinois The Department of Energy's Proposed...

  15. EA-1923: Mitigated Finding of No Significant Impact | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Mitigated Finding of No Significant Impact EA-1923: Mitigated Finding of No Significant Impact Green Energy School Wind Turbine Project on Saipan, Commonwealth of the Northern ...

  16. Community Wind Handbook/Find an Installer | Open Energy Information

    Open Energy Info (EERE)

    * Submit Permit Applications * Find an Installer * Purchase Equipment * Plan for Maintenance Find an Installer Homeowners, ranchers, and small businesses can install wind...

  17. EIS-0372: Record of Decision and Floodplain Statement of Findings...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Record of Decision and Floodplain Statement of Findings EIS-0372: Record of Decision and Floodplain Statement of Findings Bangor Hydro-Electric Company Northeast Reliability...

  18. EIS-0456: Record of Decision and Floodplain Statement of Findings...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Record of Decision and Floodplain Statement of Findings EIS-0456: Record of Decision and Floodplain Statement of Findings Cushman Hydroelectric Project, Mason County, Washington...

  19. EA-1698: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    8: Finding of No Significant Impact EA-1698: Finding of No Significant Impact Baldwin Wind Energy Center Project Baldwin Wind, LLC has requested to interconnect their proposed new...

  20. EA-1807: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1807: Finding of No Significant Impact Heartland Community College Wind Energy Project, Normal, McLean County, Illinois The Department of...

  1. EA-1819: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1819: Finding of No Significant Impact Kilowatts for Kenston Wind Energy Project, Chagrin Falls, Geauga County, Ohio The Department of Energy's...

  2. Determining physical constraints in transcriptional initiationcomplexes using DNA sequence analysis

    SciTech Connect (OSTI)

    Shultzaberger, Ryan K.; Chiang, Derek Y.; Moses, Alan M.; Eisen,Michael B.

    2007-07-01

    Eukaryotic gene expression is often under the control ofcooperatively acting transcription factors whose binding is limited bystructural constraints. By determining these structural constraints, wecan understand the "rules" that define functional cooperativity.Conversely, by understanding the rules of binding, we can inferstructural characteristics. We have developed an information theory basedmethod for approximating the physical limitations of cooperativeinteractions by comparing sequence analysis to microarray expressiondata. When applied to the coordinated binding of the sulfur amino acidregulatory protein Met4 by Cbf1 and Met31, we were able to create acombinatorial model that can correctly identify Met4 regulatedgenes.

  3. Untitled Document

    U.S. Energy Information Administration (EIA) Indexed Site

    3 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration This is an unedited Transcript of the Fall Meeting of the Energy Information Administration and the American Statistical Association Committee on Energy Statistics. The meeting took place October 16 and 17, 2003 in the Forrestal Building at 1000 Independence Ave., S.W., Washington, D.C. 20585. Most of the sessions were in 8E-089, although several break out sessions were in

  4. Untitled Document

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Revision to the Analysis of the Impacts of the Clean Power Plan May 27, 2015 Minor changes to Table 7. Nuclear power projections in three cases

    3 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration This is an unedited Transcript of the Fall Meeting of the Energy Information Administration and the American Statistical Association Committee on Energy Statistics. The meeting took place October 16 and 17, 2003 in the Forrestal

  5. Floodplain Assessment and Statement of Findings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    and Statement of Findings Floodplain Assessment and Statement of Findings Floodplain Assessment and Statement of Findings for Installation of a Renewable Energy Anaerobic Digester Facility at the University of California, Davis in Yolo County, California. PDF icon Statement of Findings More Documents & Publications Floodplain Assessment for Installation of a Renewable Energy Anaerobic Digester Facility

  6. Comparative genomics of transcriptional regulation of methionine metabolism in proteobacteria

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Leyn, Semen A.; Suvorova, Inna A.; Kholina, Tatiana D.; Sherstneva, Sofia S.; Novichkov, Pavel S.; Gelfand, Mikhail S.; Rodionov, Dmitry A.; Kuipers, Oscar P.

    2014-11-20

    Methionine metabolism and uptake genes in Proteobacteria are controlled by a variety of RNA and DNA regulatory systems. We have applied comparative genomics to reconstruct regulons for three known transcription factors, MetJ, MetR, and SahR, and three known riboswitch motifs, SAH, SAM-SAH, and SAM_alpha, in ~200 genomes from 22 taxonomic groups of Proteobacteria. We also identified two novel regulons: a SahR-like transcription factor SamR controlling various methionine biosynthesis genes in the Xanthomonadales group, and a potential RNA regulatory element with terminator-antiterminator mechanism controlling the metX or metZ genes in beta-proteobacteria. For each analyzed regulator we identified the core, taxon-specific andmore » genome-specific regulon members. By analyzing the distribution of these regulators in bacterial genomes and by comparing their regulon contents we elucidated possible evolutionary scenarios for the regulation of the methionine metabolism genes in Proteobacteria.« less

  7. Comparative genomics of transcriptional regulation of methionine metabolism in proteobacteria

    SciTech Connect (OSTI)

    Leyn, Semen A.; Suvorova, Inna A.; Kholina, Tatiana D.; Sherstneva, Sofia S.; Novichkov, Pavel S.; Gelfand, Mikhail S.; Rodionov, Dmitry A.; Kuipers, Oscar P.

    2014-11-20

    Methionine metabolism and uptake genes in Proteobacteria are controlled by a variety of RNA and DNA regulatory systems. We have applied comparative genomics to reconstruct regulons for three known transcription factors, MetJ, MetR, and SahR, and three known riboswitch motifs, SAH, SAM-SAH, and SAM_alpha, in ~200 genomes from 22 taxonomic groups of Proteobacteria. We also identified two novel regulons: a SahR-like transcription factor SamR controlling various methionine biosynthesis genes in the Xanthomonadales group, and a potential RNA regulatory element with terminator-antiterminator mechanism controlling the metX or metZ genes in beta-proteobacteria. For each analyzed regulator we identified the core, taxon-specific and genome-specific regulon members. By analyzing the distribution of these regulators in bacterial genomes and by comparing their regulon contents we elucidated possible evolutionary scenarios for the regulation of the methionine metabolism genes in Proteobacteria.

  8. Transcript of Office of Indian Energy Webinar: Inter-Tribal Technical Assistance Energy Providers Network FOA

    Broader source: Energy.gov [DOE]

    Transcript of the DOE Office of Indian Energy webinar about the Inter-Tribal Technical Assistance Energy Providers Network funding opportunity.

  9. Transcript of April 3, 2013, TAP webinar titled State Energy Strategic Planning

    Broader source: Energy.gov [DOE]

    U.S. Department of Energy (DOE) Technical Assistance Program (TAP) transcript of a TAP webinar held on April 3, 2013 and dealing with state energy strategic planning.

  10. A new chapter in the transcription SAGA (Journal Article) | SciTech...

    Office of Scientific and Technical Information (OSTI)

    SciTech Connect Search Results Journal Article: A new chapter in the transcription SAGA ... Publication Date: 2012-11-05 OSTI Identifier: 1047303 Resource Type: Journal Article ...

  11. June 2014 Electricity Advisory Committee Meeting Summaries and Transcripts Now Available

    Broader source: Energy.gov [DOE]

    The meeting summaries and transcripts for the Electricity Advisory Committee (EAC) meeting held on June 16-17, 2014, are now available.

  12. Electricity Advisory Committee Meeting, June 5-6, 2013- Meeting Summaries and Transcripts

    Broader source: Energy.gov [DOE]

    Meeting summaries and transcripts for the June 5-6, 2013 meeting of the Electricity Advisory Committee.

  13. Successful Partnerships and Lessons from the Field, Part 1, Webinar Transcript

    Broader source: Energy.gov [DOE]

    Part 1-Successful Partnerships and Lessons from the Field Webinar Transcript, from the U.S. Department of Energy Technical Assistance Program (TAP).

  14. Better Buildings Webinar Transcription- Financial Vehicles within an Integrated Energy Efficiency Program

    Broader source: Energy.gov [DOE]

    "Financial Vehicles Within an Integrated Energy Efficiency Program," webinar transcript from the U.S. Department of Energy's Better Buildings program.

  15. EA-1833: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1833: Finding of No Significant Impact The City of El Dorado Wind Energy Project, El Dorado, Butler County, Kansas The U.S. Department of...

  16. STEM Volunteer Training: Types of Volunteering and Where to Find...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Types of Volunteering and Where to Find Opportunities STEM Volunteer Training: Types of Volunteering and Where to Find Opportunities April 14, 2016 3:00PM to 4:00PM EDT

  17. ULTRA-DEEP WATER ADVISORY COMMITTEE FINDINGS AND

    Energy Savers [EERE]

    ULTRA-DEEP WATER ADVISORY COMMITTEE FINDINGS AND RECOMMENDATIONS 2014 ULTRA-DEEPWATER ADVISORY COMMITTEE COMMITTEE FINDINGS AND RECOMMENDATIONS i Table of Contents Research and Development Program Committee ............................................ 1 Sunset Committee .................................................................................................. 5 Advisory Committee Members ........................................................................... 7 ULTRA-DEEPWATER

  18. EA-1665: Final Environmental Assessment and Finding of No Significant...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    5: Final Environmental Assessment and Finding of No Significant Impact EA-1665: Final Environmental Assessment and Finding of No Significant Impact Davis-Kingman Tap 69-kV...

  19. EA-1928: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1928: Finding of No Significant Impact White-Tailed Deer Management at Brookhaven National Lab, Upton, New York Based on the information in the...

  20. EA-1809: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1809: Finding of No Significant Impact White Earth Nation Wind Energy Project II, Becker and Mahnomen Counties, MN DOE's Golden Field Office has...

  1. EA-1933: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1933: Finding of No Significant Impact Yakama Nation Drop 4 Hydropower Project, Yakama Nation Reservation, WA Based on the Final EA, DOE has...

  2. Got a Minute? Which Higgs did we find?

    ScienceCinema (OSTI)

    Stupak, John

    2014-09-15

    Dr. John Stupak talks about the discovery of the Higgs boson. Did scientists find the Higgs boson predicted back in 1964 or did they find just one of a group of particles, with the others still to be found?

  3. Got a Minute? Which Higgs did we find?

    SciTech Connect (OSTI)

    Stupak, John

    2014-06-26

    Dr. John Stupak talks about the discovery of the Higgs boson. Did scientists find the Higgs boson predicted back in 1964 or did they find just one of a group of particles, with the others still to be found?

  4. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    County, Nevada June 9, 2011 EA-1770: Finding of No Significant Impact Photovoltaic Solar Project at the Durango, Colorado Site June 3, 2011 EA-1858: Finding of No Significant...

  5. Rainbows and Leprechauns: Finding Gold in Partnerships (101)...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Rainbows and Leprechauns: Finding Gold in Partnerships (101) Rainbows and Leprechauns: Finding Gold in Partnerships (101) March 17, 2016 1:00PM to 2:30PM EDT

  6. EERE 2014 Wind Technologies Market Report Finds Wind Power at...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    2014 Wind Technologies Market Report Finds Wind Power at Record Low Prices EERE 2014 Wind Technologies Market Report Finds Wind Power at Record Low Prices August 10, 2015 - 11:00am ...

  7. EA-1656: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1656: Finding of No Significant Impact MARET Center at Crowder College, Neosho, Missouri The U.S. Department of Energy is preparing an...

  8. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    2012 EA-1812: Finding of No Significant Impact Haxtun Wind Energy Project, Logan and Phillips Counties, CO January 3, 2012 EA-1606: Finding of No Significant Impact Proposed Use...

  9. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    in Kahuku, O'ahu, Hawaii May 18, 2010 EA-1699: Finding of No Significant Impact PopeDouglas Third Combustor Expansion Project, Alexandria, Minnesota May 7, 2010 EA-1635: Finding...

  10. EA-1904: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    EA-1904: Finding of No Significant Impact Linac Coherent Light Source II at Stanford Linear Accelerator Laboratory, San Mateo, CA Based on the findings in the EA, DOE has...

  11. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Project, Zachary, Louisiana November 24, 2010 EA-1797: Finding of No Significant Impact Agua Caliente Solar Project in Yuma County, Arizona November 19, 2010 EA-1727: Finding of No...

  12. EIS-0365: Record of Decision and Floodplain Statement of Findings...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Record of Decision and Floodplain Statement of Findings EIS-0365: Record of Decision and Floodplain Statement of Findings Imperial-Mexicali 230-kV Transmission Lines DOE announces...

  13. EA-1767: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    7: Finding of No Significant Impact EA-1767: Finding of No Significant Impact Virginia State Energy Program's Cephas C&D Wastes Biomass Project, Richmond, Virginia Based on the...

  14. EA-1814: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1814: Finding of No Significant Impact City of Montpelier Combined Heat and Power, and District Energy System, Montpelier, Vermont The U.S....

  15. EA-1894: Finding of No Significant Impact | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    Albeni Falls Flexible Winter Lake Operations, Bonner, Idaho As a result of the analysis in this EA, I have the following findings. I find that: 1) the FWPO is not a substantial...

  16. EA-1726: Finding of No Significant Impact | Department of Energy

    Energy Savers [EERE]

    Finding of No Significant Impact EA-1726: Finding of No Significant Impact Kahuku Wind Power, LLC, Construction of the Kahuku Wind Power Facility in Kahuku, O'ahu, Hawaii The ...

  17. Title 16 USC 1600 Congressional Findings | Open Energy Information

    Open Energy Info (EERE)

    600 Congressional Findings Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- StatuteStatute: Title 16 USC 1600 Congressional FindingsLegal Abstract...

  18. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Findings of No Significant Impact (FONSI) Findings of No Significant Impact (FONSI) Findings of No Significant Impact are public documents issued by a Federal agency briefly presenting the reasons why an action for which the agency has prepared an environmental assessment will not have a significant effect on the human environment and, therefore, will not require preparation of an environmental impact statement. If you have any trouble finding a specific document, please contact

  19. FOIA-Summary of Findings: Circumstances surrounding decisions regarding the

    Energy Savers [EERE]

    security clearance, access and work assignments | Department of Energy FOIA-Summary of Findings: Circumstances surrounding decisions regarding the security clearance, access and work assignments FOIA-Summary of Findings: Circumstances surrounding decisions regarding the security clearance, access and work assignments FOIA request PDF icon FOIA-Summary of Findings: Circumstances surrounding decisions regarding the security clearance, access and work assignments More Documents &

  20. EA-1406: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    06: Finding of No Significant Impact EA-1406: Finding of No Significant Impact Ground Water Compliance at the New Rifle, Colorado, UMTRA Project Site On the basis of the Environmental Assessment. which analyzed relevant environmental issues and addressed the concerns of stakeholders, DOE finds that no significant impact will result from implementing the planned action at the New Rifle site. This FONSI is documented pursuant to NEPA, the Council on Environmental Quality's "Regulations for

  1. Alternative Fuels Data Center: Minnesota School District Finds Cost

    Alternative Fuels and Advanced Vehicles Data Center [Office of Energy Efficiency and Renewable Energy (EERE)]

    Savings, Cold-Weather Reliability with Propane Buses Minnesota School District Finds Cost Savings, Cold-Weather Reliability with Propane Buses to someone by E-mail Share Alternative Fuels Data Center: Minnesota School District Finds Cost Savings, Cold-Weather Reliability with Propane Buses on Facebook Tweet about Alternative Fuels Data Center: Minnesota School District Finds Cost Savings, Cold-Weather Reliability with Propane Buses on Twitter Bookmark Alternative Fuels Data Center: Minnesota

  2. EA-1795: Finding of No Significant Impact | Department of Energy

    Energy Savers [EERE]

    Finding of No Significant Impact EA-1795: Finding of No Significant Impact Loan Guarantee to Diamond Green Diesel for Construction of the Diamond Green Diesel Facility in Norco, Louisiana The Department of Energy conducted an environmental assessment that analyzed the potential environmental impacts associated with the construction and operation of a biomass-based diesel facility adjacent to the existing Valero St. Charles Refinery in Norco, Louisiana. PDF icon Finding of No Significant Impact

  3. EA-1859: Finding of No Significant Impact | Department of Energy

    Energy Savers [EERE]

    Finding of No Significant Impact EA-1859: Finding of No Significant Impact Kirkwood Community College - Kirkwood Wind Turbine Project, Cedar Rapids, Iowa The Iowa State Energy Office is proposing to provide $1,050,000 of its State Energy Program funds to Kirkwood Community College located in Cedar Rapids, Iowa, which would use these funds to purchase equipment for one 2.5-megawatt wind turbine. PDF icon Finding of No Significant Impact Kirkwood Community College Wind Turbin Project More

  4. EA-1955: Finding of No Significant Impact | Department of Energy

    Energy Savers [EERE]

    5: Finding of No Significant Impact EA-1955: Finding of No Significant Impact Campbell County Wind Farm; Campbell County, South Dakota Western Area Power Administration (Western) issued a finding of no significant impact for a proposal to interconnect, via a proposed new substation, a proposed Dakota Plains Energy, LLC, 99-megawatt wind farm near Pollock, South Dakota, to Western's existing transmission line at that location. PDF icon EA-1955 Campbell County Wind FONSI 2015-06-11.pdf More

  5. EA-1732: Floodplain Statement of Findings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    32: Floodplain Statement of Findings EA-1732: Floodplain Statement of Findings Next Autoworks Louisiana, LLC (Formerly V-Vehicle Company) and Advanced Technology Gasoline Vehicle Manufacturing Project in Monroe, Louisiana The U.S. Department of Energy proposed action is to issue a loan to Next Autoworks Louisiana, LLC for the production of an advanced technology gasoline-powered vehicle named the V-Car. PDF icon Floodplain Statement of Findings Department of Energy Loan to Next Autoworks

  6. EA-1795: Floodplain Statement of Findings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    5: Floodplain Statement of Findings EA-1795: Floodplain Statement of Findings Loan Guarantee to Diamond Green Diesel for Construction of the Diamond Green Diesel Facility in Norco, Louisiana The Department of Energy proposed action is to issue a loan guarantee to Diamond Green Diesel, LLC to support construction of a biomass-based diesel facility adjacent to the existing Valero St. Charles Refinery in Norco, Louisiana. PDF icon Attachment Floodplain Statement of Findings for Department of Energy

  7. EA-2024: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-2024: Finding of No Significant Impact Gap Material Plutonium - Transport, Receipt, and Processing Based on the analysis in the environmental assessment, the U.S. Department of Energy, National Nuclear Security Administration (NNSA), issued this finding of no significant impact for transporting up to 900 kilograms of plutonium from foreign countries to the Savannah River Site in South Carolina for storage and processing pending final disposition. NNSA will

  8. EA-1495: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    5: Finding of No Significant Impact EA-1495: Finding of No Significant Impact USEC Inc. American Centrifuge Lead Cascade Facility at Piketon, Ohio The purpose of NRC's proposed action is to authorize possession and use of source and special nuclear material at the Lead Cascade in Piketon, Ohio. PDF icon Finding of No Significant Impact for the United States Enrichment Corporation Incorporated, American Centrifuge Lead Cascade Facility at Piketon, Ohio More Documents & Publications EIS-0468:

  9. EA-1882: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Range Environmental Assessment, DOEEA-1882 (March 2011) More Documents & Publications EA-1822: Final Environmental Assessment EA-1984: Finding of No Significant Impact EA-1555...

  10. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    No Significant Impact Sunflower Wind Project, Hebron, North Dakota September 3, 2014 EA-1984: Finding of No Significant Impact Disposition of Five Signature Properties at Idaho...

  11. EVMS Training Snippet: 1.8 DOE Common EVMS Findings

    Broader source: Energy.gov [DOE]

    This EVMS Training Snippet, sponsored by the Office of Project Management (PM) discusses the common surveillance findings identified during DOE EVMS Reviews.

  12. EA-1611: Finding of No Significant Impact | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    finding of no significant impact (FONSI) for the proposed Colorado Highlands Wind Project.The FONSI describes the information Western used to determine that Western's proposal to...

  13. EA-1921: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact Silver Peak Area Geothermal Exploration Project, Esmeralda County, NV Based on the evaluations in the final EA and subject to the mitigations...

  14. EA-1183: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    EA-1183: Finding of No Significant Impact Coal-fired Diesel Generator University of ... for demonstrating the performance of a coal-fired generator at the University of ...

  15. EA-1700: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    LBNLUniversity of California Berkeley programs that focus on computational and computer science research in a new facility on the LBNL site. PDF icon Finding of No Significant...

  16. EA-0921: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    EA-0921: Finding of No Significant Impact Ambulatory Research and Education Center, Oregon Health ... the meaning of NEPA. Therefore, an environmental impact statement is not required. ...

  17. EA-2014: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    EA-2014: Finding of No Significant Impact Y-12 National Security Complex Emergency Operations Center; Oak Ridge, TN Based upon the EA, the National Nuclear Security Administration ...

  18. EA-1440: Finding of No Signficant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    EA-1440: Finding of No Signficant Impact National Renewable Energy Laboratory's South Table Mountain Complex The Proposed Action is to operate the STM complex for alternative ...

  19. Portsmouth Site Treatment Plan Director's Final Findings and...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Portsmouth Site Treatment Plan Director's Final Findings and Orders is an agreement between the Ohio EPA and DOE approving the Site Treatment Plan and setting forth guidelines for ...

  20. EA-1797: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    EA-1797: Finding of No Significant Impact Agua Caliente Solar Project in Yuma County, ... transmission line proposed by Agua Caliente Solar, LLC in Yuma County, Arizona. ...

  1. Rainbows and Leprechauns: Finding Gold in Partnerships (101)

    Broader source: Energy.gov [DOE]

    Better Buildings Residential Network Peer Exchange Call Series: Rainbows and Leprechauns: Finding Gold in Partnerships (101), March 17, 2016, call slides and discussion summary.

  2. CLASS DEVIATION FINDINGS AND DETERMINATION TO IMPLEMENT AN APPROPRIATI...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    PROVISION RELATED TO INTERNAL CONFIDENTIALITY AGREEMENTS AND THE REPORTING OF FRAUD, WASTE, AND ABUSE Findings I. Section 743 of Division E, Title VII of the Consolidated...

  3. Finding of No Significant Impact, Federal Register, 73 FR 2017...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    73 FR 2017, January 11, 2008 Document details the finding of no significant impact of the Energy-Efficient Performance Requirements for New Federal Commercial and Residential...

  4. Findings of No Significant Impact (FONSI) | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    Ridge Reservation for the biological control of the emerald ash borer, a nonnative invasive beetle. May 1, 2015 EA-1796: Finding of No Significant Impact Loan Guarantee to...

  5. EA-1685: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of No Significant Impact EA-1685: Finding of No Significant Impact Experimental Coal Flow Program at Clemson University The Western Area Power Administration (Western) is the...

  6. PPPL physicists find clue to formation of magnetic fields around...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    results, in the range in which they are done, strongly suggest that such dynamo action is possible." These findings might lead to greater understanding of the behavior of...

  7. EA-1696: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    EA-1696: Finding of No Significant Impact Pantex Renewable Energy Project, Amarillo, Texas The NNSA has prepared an Environmental Assessment to analyze the potential...

  8. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    and Heating Facilities at the Savannah River Site, South Carolina June 11, 2008 EA-1613: Finding of No Significant Impact Proposed High Explosive Pressing Facility Pantex...

  9. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of No Significant Impact Proposed Gas Main and Distribution System Upgrade, Pantex Plant, Amarillo, Texas August 26, 2005 EA-1542: Finding of No Significant Impact Burleigh County...

  10. EA-1974: Finding of No Significant Impact and Mitigation Action...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Bonneville Power Administration issued a finding of no significant impact (FONSI) for the proposed restoration of a tidal marsh in the Columbia River Estuary, near Astoria in ...

  11. Oregon 401 Evaluation and Findings Checklist | Open Energy Information

    Open Energy Info (EERE)

    401 Evaluation and Findings Checklist Jump to: navigation, search OpenEI Reference LibraryAdd to library PermittingRegulatory Guidance - Checklist: Oregon 401 Evaluation and...

  12. EA-1796: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    near Gillespie, Arizona, DOEEA-1796 (February 2011) More Documents & Publications EA-1796: Final Environmental Assessment EA-1796: Finding of No Significant Impact MESQUITE

  13. EA-1952: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ...energy.govnode607866. Download Document PDF icon EA-1952: Finding of No Significant Impact More Documents & Publications EA-1952: Draft Environmental Assessment EA-1952: Final

  14. EA-1931: Finding of No Significant Impact and Mitigation Action...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Bonneville Power Administration issued a finding of no significant impact and a ... More Documents & Publications EA-1931: Draft Environmental Assessment EA-1931: Final ...

  15. EA-1927: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    DOE's PortsmouthPaducah Project Office issued a Finding of No Significant Impact ... More Documents & Publications EA-1927: Draft Environmental Assessment EA-1927: Final ...

  16. EA-1979: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    icon EA-1979-FONSI-2015.pdf More Documents & Publications EA-1979: Draft Environmental Assessment EA-1979: Final Environmental Assessment EA-1955: Finding of No Significant Impact

  17. EA-1836: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    to facilitiate installation and operations of a high-efficiency natural-gas-fired cogeneration facility - would result in no significant adverse impacts. Finding of No...

  18. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    EA-1754: Finding of No Significant Impact Public Service Company of New Mexico, Photovoltaic Plus Battery for Simultaneous Voltage Smoothing and Peak Shifting Project,...

  19. EA-1719: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Impact EA-1719: Finding of No Significant Impact Novolyte Technologies, Inc. Electric Drive Vehicle Battery and Component Manufacturing Initiative Project, Zachary, Louisiana DOE...

  20. EA-1723: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Impact EA-1723: Finding of No Significant Impact General Motors LLC Electric Drive Vehicle Battery and Component Manufacturing Initiative Application, White Marsh,...

  1. EA-1613: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    EA-1613: Finding of No Significant Impact Proposed High Explosive Pressing Facility Pantex Plant, Amarillo, Texas The proposed action is to construct a new facility that would ...

  2. EA-1777: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Significant Impact EA-1777: Finding of No Significant Impact Lincoln Electric's Wind Energy Project in Euclid, OH DOE prepared an environmental assessment analyzing the potential...

  3. EA-1426: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    EA-1426: Finding of No Significant Impact Linac Coherent Light Source Project, Stanford ... Coherent Light Source (LCLS) at the Stanford Linear Accelerator Center (SLAC), ...

  4. EA-1762: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of No Significant Impact EA-1762: Finding of No Significant Impact Wellford Landfill Methane and Greenhouse Gas to Energy Project, Spartanburg County, South Carolina The...

  5. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Uranium July 1, 2009 EA-1645: Finding of No Significant Impact Loan Guarantee for Sage Electrochromics SageGlass High Volume Manufacturing Facility in Faribault, MN June 26,...

  6. EA-1683: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    EA-1683: Finding of No Significant Impact Abengoa Solar Inc., the Solana Thermal Electric Power Project near Gila Bend, Arizona The Department of Energy has conducted an ...

  7. EA-1907: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Impact EA-1907: Finding of No Significant Impact Western Plains Energy, LLC Biogas Anaerobic Digester Facility, Oakley, Kansas Based on the analysis in the Final EA and...

  8. EA-1770: Finding of No Significant Impact | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    of the National Environmental Policy Act (NEPA). Finding of No Significant Impact Photovoltaic Solar Project at the Durango, Colorado, Disposal Site, La Plata County, DOEEA 1770...

  9. EA-1837: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact Performance Verification Laboratory, Morgantown, West Virginia Based on the analyses in the environmental assessment, DOE determined that its...

  10. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of the Department of Energy, Waste Isolation Pilot Plant (WIPP) in Eddy County, New Mexico January 13, 2010 EA-1651: Finding of No Significant Impact U-233 Material...

  11. EA-1539: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Significant Impact EA-1539: Finding of No Significant Impact North Area Right-of-Way Maintenance, California Western Area Power Administration proposes to revise its operation and...

  12. Main Page Main Page: Find energy information and data. Share...

    Open Energy Info (EERE)

    Main Page Main Page: Find energy information and data. Share knowledge. Connect with people. energy datasets energy community Main Page energy information open data 0...

  13. EA-1864: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Impact EA-1864: Finding of No Significant Impact Electrical District 5 - Palo Verde Hub Project The U.S. Department of Energy (DOE) Western Area Power Administration...

  14. *** CANCELED *** SCIENCE ON SATURDAY- "Finding a Needle in A...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ON SATURDAY- "Finding a Needle in A (Genomic) Haystack or How Can Computers Help Cure Cancer" Professor Olga G. Troyanskaya Lewis-Sigler Institute for Integrative Genomics &...

  15. Innovative Financing Solutions: Finding Money for Your Energy Efficiency Projects

    Broader source: Energy.gov [DOE]

    Provides an overview of how to find funding for your energy efficiency projects focusing on l-p agreements. Author: Energy Star

  16. FOIA-Summary of Findings: Circumstances surrounding decisions...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    the security clearance, access and work assignments FOIA-Summary of Findings: Circumstances surrounding decisions regarding the security clearance, access and work assignments ...

  17. ULTRA-DEEP WATER ADVISORY COMMITTEE FINDINGS AND

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... ULTRA-DEEPWATER ADVISORY COMMITTEE COMMITTEE FINDINGS AND RECOMMENDATIONS 7 Advisory Committee Members Dr. George Cooper* University of California, Berkeley Dr. Quenton R. Dokken ...

  18. Commercial Building Energy Asset Score: Pilot Findings and Program Update

    Broader source: Energy.gov [DOE]

    The webinar was held on April 16, 2014, to share the findings from the 2013 Pilot and provide a program update on the Commercial Building Energy Asset Score.

  19. Microsoft Word - Summary of RCA findings and lessons learned...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Work Planning and Scheduling System project Root cause analysis findings and lessons learned Summary BPA stopped the Work Planning and Scheduling System project in February 2012...

  20. Finding Large Aperture Fractures in Geothermal Resource Areas...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Long-Offset Surface Seismic Survey Finding Large Aperture Fractures in Geothermal Resource Areas Using a Three-Component Long-Offset Surface Seismic Survey DOE ...

  1. Scientist finds new way to predict heat layer troublemaker |...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Boon to fusion: Scientist finds new way to predict heat layer troublemaker By John ... But heat inevitably flows through the system and becomes separated, or scraped off, from ...

  2. Scientist finds new way to predict heat layer troublemaker |...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Scientist finds new way to predict heat layer troublemaker By John Greenwald August 27, ... But heat inevitably flows through the system and becomes separated, or scraped off, from ...

  3. DOE issues Finding of No Significant Impact for the Environmental...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    issues Finding of No Significant Impact for the Environmental Assessment on Resumption of Transient Testing of Nuclear Fuels and Materials at Idaho National Laboratory DOE issues ...

  4. EA-1628: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Iowa. PDF icon Mitigation Action Plan for the Environmental Assessment, Notice of Wetlands Involvement and Finding of No Significant Impact for Construction and Operation of a...

  5. Extracellular Matrix-Regulated Gene Expression RequiresCooperation of SWI/SNF and Transcription Factors

    SciTech Connect (OSTI)

    Xu, Ren; Spencer, Virginia A.; Bissell, Mina J.

    2006-05-25

    Extracellular cues play crucial roles in the transcriptional regulation of tissue-specific genes, but whether and how these signals lead to chromatin remodeling is not understood and subject to debate. Using chromatin immunoprecipitation (ChIP) assays and mammary-specific genes as models, we show here that extracellular matrix (ECM) molecules and prolactin cooperate to induce histone acetylation and binding of transcription factors and the SWI/SNF complex to the {beta}- and ?-casein promoters. Introduction of a dominant negative Brg1, an ATPase subunit of SWI/SNF complex, significantly reduced both {beta}- and ?-casein expression, suggesting that SWI/SNF-dependent chromatin remodeling is required for transcription of mammary-specific genes. ChIP analyses demonstrated that the ATPase activity of SWI/SNF is necessary for recruitment of RNA transcriptional machinery, but not for binding of transcription factors or for histone acetylation. Coimmunoprecipitation analyses showed that the SWI/SNF complex is associated with STAT5, C/EBP{beta}, and glucocorticoid receptor (GR). Thus, ECM- and prolactin-regulated transcription of the mammary-specific casein genes requires the concerted action of chromatin remodeling enzymes and transcription factors.

  6. EV Everywhere: Find Electric Vehicle Models | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Find Electric Vehicle Models EV Everywhere: Find Electric Vehicle Models Search Car: Year: -- ALL -- Make: -- ALL -- Market Class: -- ALL -- All-Electric Range: Min -- ALL -- 10 miles 20 miles 30 miles 40 miles 50 miles 60 miles 70 miles 80 miles 90 miles 100 miles 110 miles 120 miles 130+ miles Gasoline Back-Up Available: -- ALL -- No Yes Reset To find out if a plug-in electric vehicle (EV) will work for you, use the menus to the left to sort the available EV models on the market by year, make,

  7. Finding renewable energy materials using one screensaver at a time:

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Find us at the Bay Area Maker Faire Find us at the Bay Area Maker Faire Find us at the Bay Area Maker Faire It's dubbed the "Greatest Show (and Tell) on Earth." Of course we'll be there! From satellites to a 3D-printed Jeep to remote controlled robots to carbon capture microbeads, we'll be displaying some of the coolest technologies from across the Energy Department. We also want to meet innovators like you! Representatives from the Department of Energy's National Labs, the Office of

  8. How to Find Ames Laboratory | The Ames Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    How to Find Ames Laboratory Finding the Ames Laboratory The Ames Laboratory has it's main administrative offices in the Technical and Administrative Services Facility (TASF) building on the Iowa State University Campus. For directions, please use the GPS coordinates 42.0305° N, 93.6482° W. For directions from the Des Moines Airport please use the link with the following directions: https://goo.gl/maps/c6SOK Finding the Ames Laboratory on the ISU Campus Image Click on the map to download a

  9. EA-1941: Finding of No Significant Impact (FONSI) | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    BPA issued a Finding of No Significant Impact for proposed improvements to 13 miles of access roads for its existing 115-kV Boyer-Tillamook No. 1 Transmission Line. PDF icon ...

  10. Scientist Finds Nature and Geometry Dancing to the Same Tune...

    Office of Science (SC) Website

    The covering problem is a puzzle that involves overlapping spheres. The objective is to find the most efficient way to arrange overlapping spheres so that they just cover a ...

  11. EA-1772: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    The proposed action consists of constructing a road for limited year-round use with the ability for trucks traveling in opposite directions to pass. PDF icon Finding of No ...

  12. EA-1948: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    DOE's Western Area Power Administration issued a finding of no significant impact for a proposal to rebuild and upgrade two parallel 4.8-mile transmission lines between the Gila ...

  13. EA-1948: Revised Finding of No Significant Impact | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    On March 21, 2014, DOE's Western Area Power Administration issued a finding of no significant impact (FONSI) for a proposal to rebuild and upgrade two parallel 4.8-mile ...

  14. EA-1871: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    EA-1871: Finding of No Significant Impact Energy Efficiency Design Standards for New Federal Commercial and Multi-Family High-Rise Residential Buildings and New Federal Low-Rise ...

  15. EA-1812: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    DOE has issued a Finding of No Significant Impact (FONSI) based on the information in the Final EA, FONSI, and the Supplement Analysis (SA) prepared to address changes in the ...

  16. EA-1973: Finding of No Significant Impact and Mitigation Action...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    the project webpage. PDF icon EA-1973-FONSI-MAP-2015 More Documents & Publications EA-1973: Draft Environmental Assessment EA-1901: Finding of No Significant Impact EA-1518: Final

  17. EA-1964: FInding of No Significant Impact | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    13, DOE adopted the NSF EA and issued a finding of no significant impact regarding NEON Project research activities on the Oak Ridge Reservation. EA-1964-FONSI-2013.pdf More...

  18. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Winnebago County, Illinois December 2, 2011 EA-1697: Finding of No Significant Impact Right-of-Way Maintenance in the San Joaquin Valley, California November 10, 2011 EA-1862:...

  19. EA-1760: Finding of No Significant Impact | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    of a commercial-scale plant to produce intermediate anode material for high-performance lithium-ion batteries would result in no significant adverse impacts. Finding of No...

  20. EA-1711: Finding of No Significant Impact | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    America, Inc to construct and operate a high-volume manufacturing plant to make advanced lithium-ion cells and batteries, would result in no significant adverse impacts. Finding...

  1. Finding Six-Figure ROI From Energy Efficiency | Department of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Six-Figure ROI From Energy Efficiency Finding Six-Figure ROI From Energy Efficiency September 28, 2010 - 10:20am Addthis Kevin Craft What are the key facts? Recovery Act funded ...

  2. EA-1942: Finding of No Significant Impact | Department of Energy

    Energy Savers [EERE]

    DOE adopted FERC's EA and issued a Finding of No Significant Impact. PDF icon EA-1942-FONSIl2014.pdf More Documents & Publications Summary of LNG Export Applications of the Lower ...

  3. EA-1888: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of No Significant Impact EA-1888: Finding of No Significant Impact Old Town Fuel and Fiber Proposed Demonstration-Scale Integrated Biorefinery in Old Town, MN DOE has determined...

  4. EA-2002: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    EA-2002: Finding of No Significant Impact Right-of-Way Application for the Tucson-Apache 115-kV Transmission Line, Pima County, Arizona Western Area Power Administration issued a ...

  5. Indoor Thermal Factors and Symptoms in Office Workers: Findings...

    Office of Scientific and Technical Information (OSTI)

    from the U.S. EPA BASE Study Citation Details In-Document Search Title: Indoor Thermal Factors and Symptoms in Office Workers: Findings from the U.S. EPA BASE Study You ...

  6. DOE ZERH Webinar: Tour of Zero, Helping Customers Find Better...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Are you interested in helping homebuyers find a better home at a lower cost? The DOE Tour of ... This national campaign is where you come in. DOE needs Innovation partners vested in ...

  7. DOE issues Finding of No Significant Impact for the Environmental...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    DOE issues Finding of No Significant Impact for the Environmental Assessment on Resumption of Transient Testing of Nuclear Fuels and Materials at Idaho National Laboratory The U.S. ...

  8. Finding Large Aperture Fractures in Geothermal Resource Areas...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    A Three-Component Long-Offset Surface Seismic Survey, PSInSAR, and Kinematic Analysis Finding Large Aperture Fractures in Geothermal Resource Areas Using A Three-Component Long-Off...

  9. Transcript of March 28, 2013, TAP webinar titled Internal Benchmarking Outreach and Data Collection Techniques

    Broader source: Energy.gov [DOE]

    U.S. Department of Energy (DOE) Technical Assistance Program (TAP) transcript of a TAP webinar held on March 28, 2013 and dealing with internal benchmarking outreach and data collection techniques.

  10. Transcript of April 11, 2013, TAP webinar titled Financing Energy Upgrades for K-12 School Districts

    Broader source: Energy.gov [DOE]

    U.S. Department of Energy (DOE) Technical Assistance Program (TAP) transcript of a TAP webinar held on April 11, 2013 and dealing with how to finance energy efficiency upgrades for K-12 school districts.

  11. Resources for Handling Transcripts | U.S. DOE Office of Science...

    Office of Science (SC) Website

    The transcript PDF does not have to be a color copy. If applicant does not have a personal scanner or need assistance formatting the file, applicant should consider visiting a...

  12. Impact of the N-Terminal Domain of STAT3 in STAT3-Dependent Transcript...

    Office of Scientific and Technical Information (OSTI)

    Impact of the N-Terminal Domain of STAT3 in STAT3-Dependent Transcriptional Activity Citation Details In-Document Search Title: Impact of the N-Terminal Domain of STAT3 in ...

  13. Neutrons find "missing" magnetism of plutonium

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Neutrons find "missing" magnetism of plutonium Neutrons find "missing" magnetism of plutonium Groundbreaking work at two Department of Energy national laboratories has confirmed plutonium's magnetism, which scientists have long theorized but have never been able to experimentally observe. July 10, 2015 Doug Abernathy, left, ARCS instrument scientist at Oak Ridge National Laboratory, and Marc Janoschek, Los Alamos National Laboratory, prepare their sample for experiments at

  14. Two Independent Assessments Find the Department of Energy's Yucca Mountain

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Project is on Track | Department of Energy Independent Assessments Find the Department of Energy's Yucca Mountain Project is on Track Two Independent Assessments Find the Department of Energy's Yucca Mountain Project is on Track December 13, 2007 - 4:44pm Addthis WASHINGTON, DC - The U.S. Department of Energy (DOE) Director of the Office of Civilian Radioactive Waste Management (OCRWM) today released two independent assessments addressing areas critical to the overall success of the Yucca

  15. Find Financing for Energy Efficiency Upgrades | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Find Financing for Energy Efficiency Upgrades Find Financing for Energy Efficiency Upgrades Photo of a block in lower downtown Denver that is part of Living City Block, a DOE Commercial Building Partnerships participant. Financing need not be a barrier to improving energy efficiency in your commercial building. There are federal, state, and local and tax incentives for energy efficiency upgrades, and other financial opportunities to help you meet your energy goals. The U.S. Department of

  16. Find Product Categories Covered by Efficiency Programs | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Products & Technologies » Energy-Efficient Products » Find Product Categories Covered by Efficiency Programs Find Product Categories Covered by Efficiency Programs Legend ★ ENERGY STAR △ FEMP Designated O EPEAT ♦ Low Standby Power X WaterSense Federal agencies are required to purchase energy-efficient products. To help buyers meet these requirements, the Federal Energy Management Program (FEMP) maintains acquisition guidance for the following product categories, which are

  17. DOE Issues Finding of No Significant Impact and Final Environmental

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Assessment on Road Project U.S. DEPARTMENT OF ENERGY IDAHO FALLS, IDAHO, 83403 News Media Contact: Tim Jackson (208) 526-8484 For Immediate Release Thursday August 12, 2010 DOE Issues Finding of No Significant Impact and Final Environmental Assessment on Road Project The U.S. Department of Energy has issued a Finding of No Significant Impact stemming from an environmental assessment it performed to examine the potential environmental impacts of building an internal road to safely ship

  18. EA-1985: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1985: Finding of No Significant Impact Virginia Offshore Wind Technology Advancement Project on the Atlantic Outer Continental Shelf Offshore Virginia DOE is proposing to fund Virginia Electric and Power Company's Virginia Offshore Wind Technology Advancement Project (VOWTAP). The proposed VOWTAP project consists of design, construction and operation of a 12 megawatt offshore wind facility located approximately 24 nautical miles off the coast of Virginia

  19. EA-2001: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-2001: Finding of No Significant Impact Energy Efficiency Standards for New Federal Commercial and Multi-Family High-Rise Residential Buildings' Baseline Standards Update (RIN 1904-AD39) Based upon the EA, DOE has determined that revising the Federal building energy efficiency standards for commercial and multi-family high-rise residential buildings to ASHRAE 90.1-2013 would not be a major Federal action significantly affecting the quality of the human

  20. EA-2004: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-2004: Finding of No Significant Impact The Seneca Nation Wind Turbine Project, Cattaraugus Territory, Erie County, New York Based upon the EA, DOE has determined that authorizing the expenditure of federal funding to the Seneca Nation of Indians to design, permit, and construct up to a 2.0 megawatt wind turbine on Tribal common lands in the Cattaraugus Territory, New York would not be a major Federal action significantly affecting the quality of the human

  1. EA-2017: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-2017: Finding of No Significant Impact Braddock Locks and Dam Hydro Electric Project Based upon the review of the EA prepared by the Federal Energy Regulatory Commission (FERC) and adopted by DOE pursuant to the requirements of the National Environmental Policy Act (NEPA), DOE has determined that authorizing the expenditure of federal funding to Hydro Green Energy, LLC to fabricate, install, and operate one interchangeable Modular Bulb Turbine (MBT), which

  2. Neutrons find "missing" magnetism of plutonium

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Neutrons find "missing" magnetism of plutonium Alumni Link: Opportunities, News and Resources for Former Employees Latest Issue:September 2015 all issues All Issues » submit Neutrons find "missing" magnetism of plutonium Groundbreaking work at two Department of Energy national laboratories has confirmed plutonium's magnetism, which scientists have long theorized but have never been able to experimentally observe. September 1, 2015 Doug Abernathy, left, ARCS instrument

  3. Moving Industry Forward: Finding the Environmental Opportunity in Biochar

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Moving Industry Forward: Finding the Environmental Opportunity in Biochar Moving Industry Forward: Finding the Environmental Opportunity in Biochar Print Thursday, 12 September 2013 08:41 The Environmental Protection Agency (EPA) has been investigating biofuels for several years now as part of its mission of moving the nation toward a cleaner energy future. Recently, EPA scientists have refocused their research on a promising biofuel byproduct-biochar, which is the solid material that's left

  4. From pandemic preparedness to biofuel production: Tobacco finds its

    Office of Scientific and Technical Information (OSTI)

    biotechnology niche in North America (Journal Article) | DOE PAGES From pandemic preparedness to biofuel production: Tobacco finds its biotechnology niche in North America Title: From pandemic preparedness to biofuel production: Tobacco finds its biotechnology niche in North America As part of my NSD Innovation awarded funds (95470 Powell Innovation: charge code N38540) one my deliverables was a review article for journal submission summarizing my work on this project. My NSD Innovation

  5. DOE issues Finding of No Significant Impact for the Environmental

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Assessment on Resumption of Transient Testing of Nuclear Fuels and Materials at Idaho National Laboratory | Department of Energy issues Finding of No Significant Impact for the Environmental Assessment on Resumption of Transient Testing of Nuclear Fuels and Materials at Idaho National Laboratory DOE issues Finding of No Significant Impact for the Environmental Assessment on Resumption of Transient Testing of Nuclear Fuels and Materials at Idaho National Laboratory February 28, 2014 - 12:00pm

  6. DOE issues Finding of No Significant Impact for the Environmental

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Assessment on Resumption of Transient Testing of Nuclear Fuels and Materials at Idaho National Laboratory | Department of Energy DOE issues Finding of No Significant Impact for the Environmental Assessment on Resumption of Transient Testing of Nuclear Fuels and Materials at Idaho National Laboratory DOE issues Finding of No Significant Impact for the Environmental Assessment on Resumption of Transient Testing of Nuclear Fuels and Materials at Idaho National Laboratory February 28, 2014 -

  7. CREED: Helping Colorado Cleantech Startups Find the Money - News Releases |

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    NREL CREED: Helping Colorado Cleantech Startups Find the Money January 19, 2012 The Colorado Center for Renewable Energy Economic Development (CREED) at the U.S. Department of Energy's National Renewable Energy Laboratory (NREL) invites cleantech entrepreneurs to attend the next event in its Entrepreneur Series. The Jan. 26 class, "Financing Your Cleantech Startup," will provide cleantech entrepreneurs with an overview of how to find capital for a startup. "If you are in

  8. EA-1414: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    4: Finding of No Significant Impact EA-1414: Finding of No Significant Impact Implementation of the Authorized Limits Process for Waste Acceptance at the C-746-U Landfill, Paducah Gaseous Diffusion Plant, Paducah, Kentucky DOE plans to implement the authorized limits process for determining the acceptability of waste containing low levels of residual radioactive materials on both a surface-contaminated and a volumetric basis in accordance with established DOE requirements for disposal at the

  9. EA-1236: Finding of No Significant Impact | Department of Energy

    Energy Savers [EERE]

    Finding of No Significant Impact EA-1236: Finding of No Significant Impact Preparation for Transfer of Ownership of Naval Petroleum Reserve No. 3, Natrona County, Wyoming Based on the analysis of the Sitewide Environmental Assessment for Naval Petroleum Reserve No. 3 (NPR-3) DOE has determined that the proposed action to conduct activities in anticipation of possible transfer of NPR-3 out of Federal operation is not a major Federal action significantly affecting the quality of the human

  10. EA-1331: Finding of No Significant Impact | Department of Energy

    Energy Savers [EERE]

    1: Finding of No Significant Impact EA-1331: Finding of No Significant Impact Remediation of Subsurface and Groundwater Contamination at the Rock Springs in situ Oil Shale Retort Site Based on the analysis and information provided in the EA, DOE has determined that the proposed Federal action, to conduct air sparging at the Rock Springs in situ Oil Shale Retort Site in Sweetwater County, Wyoming, does not constitute a major Federal action significantly affecting the quality of the human

  11. EA-1570: Final Environmental Assessment and Finding of No Significant

    Energy Savers [EERE]

    Impact | Department of Energy Assessment and Finding of No Significant Impact EA-1570: Final Environmental Assessment and Finding of No Significant Impact Construction and Operation of Neutrinos at the Main Injector Off-Axis Electron Neutrino Appearance Experiment (NOvA) at the Fermi National Accelerator Laboratory, Batavia, Illinois, and St. Louis County, Minnesota This Environmental Assessment (DOE/EA-1570) provides information and analysis of proposed U.S. Department of Energy activities

  12. EA-1602: Finding of No Significant Impact | Department of Energy

    Energy Savers [EERE]

    2: Finding of No Significant Impact EA-1602: Finding of No Significant Impact Alternative Intake Project Transmission Line and Interconnection, California This notice provides Western Area Power Administration's National Environmental Poilcy Act determination regarding Western's proposal to provide an electric transmission line and interconnection to Contra Costa Water District's Alternative Intake Project (AIP) located in the Sacramento-San Joaquin Delta, within San Joaquin and Contra Costa

  13. EA-1891: Finding of No Significant Impact | Department of Energy

    Energy Savers [EERE]

    891: Finding of No Significant Impact EA-1891: Finding of No Significant Impact Alvey-Fairview Transmission Line Rebuild Project, Oregon Based on the information in the EA, BPA determined that the proposed action is not a major federal action significantly affecting the quality of the human environment within the meaning of NEPA. Therefore, an EIS will not be prepared and BPA is issuing this FONSI for the proposed action. PDF icon EA-1891-FONSI-2014.pdf More Documents & Publications EA-1891:

  14. NERSC Resources Help Find Roots of MJO Modeling Mismatches

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    NERSC Resources Help Find Roots of MJO Modeling Mismatches NERSC Resources Help Find Roots of MJO Modeling Mismatches April 18, 2016 MJOmodeling From Calcutta, India, to Seattle, Wash. and beyond, the MJO can bring storms and plenty of rain in wet years. PNNL researchers are using field data and NERSC supercomputers to better model how the MJO operates and understand its interaction regional weather systems around the world. Image: PNNL An Alaska-sized pulse of clouds and precipitation

  15. EA-1995: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    95: Finding of No Significant Impact EA-1995: Finding of No Significant Impact Trestle Bay Ecosystem Restoration Project, Clatsop County, Oregon The U.S. Army Corps of Engineers prepared a Feasibility Study and EA that evaluates the potential environmental impacts of a proposal to improve estuary habitat in Trestle Bay, located at the mouth of the Columbia River and the Pacific Ocean. DOE's Bonneville Power Administration (BPA), a cooperating agency, proposes to partially fund the project. BPA

  16. EA-2036: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-2036: Finding of No Significant Impact Sabine Pass Liquefaction Project (design optimization); Cameron Parish, Louisiana In January 2014, the Federal Energy Regulatory Commission (FERC) issued an EA to analyze the potential environmental impacts associated with an application to amend FERC's April 2012 authorization of the Sabine Pass Liquefaction Project in order to optimize its design and operation. In April 2015, Sabine Pass Liquefaction, LLC, and Sabine

  17. EA-1793: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    Finding of No Significant Impact EA-1793: Finding of No Significant Impact Replacement Capability for Disposal of Remote-Handled Low-Level Radioactive Waste Generated at the Department of Energy's Idaho Site DOE prepared an environmental assessment (EA), for the Replacement Capability for the Disposal of Remote-Handled Low-Level Radioactive Waste Generated at the Department of Energy's Idaho Site to evaluate potential environmental impacts related to replacement capability options. Based on the

  18. Finding the Right Filling Station for Alternative Vehicles Now Easier

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Finding the Right Filling Station for Alternative Vehicles Now Easier For more information contact: e:mail: Public Affairs Golden, Colo., May 5, 1999 — A new online interactive computer program is taking the guesswork out of finding the fuel needed by the thousands of alternative vehicles on the road today in the United States. The program, called the Alternative Fuel Refueling Station Locator, was developed by the U.S. Department of Energy's (DOE) Alternative Fuels Data Center (AFDC). The AFDC

  19. Study finds surprising variability in shape of Van Allen Belts

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Study finds surprising variability in shape of Van Allen Belts Study finds surprising variability in shape of Van Allen Belts Understanding the shape and size of the belts, which shrink and swell in response to magnetic storms coming from the sun, is crucial for protecting our technology in space. February 23, 2016 1. The traditional idea of the radiation belts includes a larger, more dynamic outer belt and a smaller, more stable inner belt with an empty slot region separating the two. However,

  20. Curiosity rover finds evidence of Mars' primitive continental crust

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Curiosity rover finds evidence of Mars' primitive continental crust Curiosity rover finds evidence of Mars' primitive continental crust This is the first discovery of a potential "continental crust" on Mars. July 13, 2015 Igneous clast named Harrison embedded in a conglomerate rock in Gale crater, Mars, shows elongated light-toned feldspar crystals. The mosaic merges an image from Mastcam with higher-resolution images from ChemCam's Remote Micro-Imager. Credit:

  1. Transcript of How to Navigate the New and Improved State and Local Solution

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Center Webinar | Department of Energy Transcript of How to Navigate the New and Improved State and Local Solution Center Webinar Transcript of How to Navigate the New and Improved State and Local Solution Center Webinar So, starting with Weatherization and Intergovernmental Programs, who we are and what we do, our mission is primarily to accelerate the deployment of energy efficiency and renewable energy technologies, working primarily with our stakeholders, state and local governments.

  2. Impact of the N-Terminal Domain of STAT3 in STAT3-Dependent Transcriptional

    Office of Scientific and Technical Information (OSTI)

    Activity (Journal Article) | SciTech Connect Impact of the N-Terminal Domain of STAT3 in STAT3-Dependent Transcriptional Activity Citation Details In-Document Search Title: Impact of the N-Terminal Domain of STAT3 in STAT3-Dependent Transcriptional Activity Authors: Hu, Tiancen ; Yeh, Jennifer E. ; Pinello, Luca ; Jacob, Jaison ; Chakravarthy, Srinivas ; Yuan, Guo-Cheng ; Chopra, Rajiv ; Frank, David A. [1] ; DFCI) [2] ; Harvard) [2] ; IIT) [2] ; BWH) [2] + Show Author Affiliations

  3. DOE Zero Energy Ready Home: Better Business for Builders Webinar Transcript

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    | Department of Energy Home: Better Business for Builders Webinar Transcript DOE Zero Energy Ready Home: Better Business for Builders Webinar Transcript Below is the text version of the webinar, DOE Zero Energy Ready Home: Better Business for Builders, presented in August 2014. GoToWebinar voice: The broadcast is now starting. All attendees are in listen- only mode. Lindsay Parker: ... here today, and welcome to the Department of Energy Zero Energy Ready Home Technical Training Webinar

  4. Curved track segment finding using Tiny Triplet Finder (TTF)

    SciTech Connect (OSTI)

    Wu, Jin-Yuan; Wang, M.; Gottschalk, E.; Shi, Z.; /Fermilab

    2006-11-01

    We describe the applications of a track segment recognition scheme called the Tiny Triplet Finder (TTF) that involves the grouping of three hits satisfying a constraint forming of a track segment. The TTF was originally developed solving straight track segment finding problem, however, it is also suitable in many curved track segment finding problems. The examples discussed in this document are among popular detector layouts in high-energy/nuclear physics experiments. Although it is not practical to find a universal recipe for arbitrary detector layouts, the method of the TTF application is illustrated via the discussion of the examples. Generally speaking, whenever the data item to be found in a pattern recognition problem contains two free parameters, and if the constraint connecting the measurements and the two free parameters has an approximate shift invariant property, the Tiny Triplet Finder can be used.

  5. Finding & Sharing Information about Energy Efficiency | Department of

    Energy Savers [EERE]

    of Energy Find Energy Education Resources in a Snap with New Search Tool Find Energy Education Resources in a Snap with New Search Tool January 7, 2015 - 1:46pm Addthis Fun learning activities, like the <a href="http://www1.eere.energy.gov/education/pdfs/solar_pizza_oven_box.pdf">pizza box solar oven exercise</a> above, are just a few of the resources that can be found using the new <a href="http://energy.gov/education-toolbox/search">Education

  6. Research Finds Vitamin D Deficiency Affects Bone Quality

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Research Finds Vitamin D Deficiency Affects Bone Quality Research Finds Vitamin D Deficiency Affects Bone Quality Print Wednesday, 30 April 2014 00:00 Vitamin D deficiency is a widespread medical condition that plays a major role in human bone health. Scientists know that a lack of vitamin D can cause bone diseases such as rickets and osteomalacia. Now a team of researchers working at the ALS has also found that vitamin D deficiency plays a significant role in the bone-aging process. Low levels

  7. EA-1528: Finding of No Significant Impact | Department of Energy

    Energy Savers [EERE]

    Finding of No Significant Impact EA-1528: Finding of No Significant Impact Storage of Tritium-Producing Burnable Absorber Rods in K-Area Transfer Bay at the Savannah River Site The proposed action is to implement the storage of TPBARs in the K-Area dry storage Transfer Bay for a period of up to two years. The Transfer Bay is proposed for the storage of TPBARs in a 10 CFR 71-certified shipping package that offers a high degree of protection for the tritiated rods. The shipping cask, its outer ISO

  8. Finding the needle in the haystack: Algorithms for conformational optimization

    SciTech Connect (OSTI)

    Andricioaei, I.; Straub, J.E.

    1996-09-01

    Algorithms are given for comformational optimization of proteins. The protein folding problems is regarded as a problem of global energy mimimization. Since proteins have hundreds of atoms, finding the lowest-energy conformation in a many-dimensional configuration space becomes a computationally demanding problem.{copyright} {ital American Institute of Physics.}

  9. NREL analysis finds tax credit extensions can impact renewable energy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    deployment and electric sector CO2 emissions - News Releases | NREL analysis finds tax credit extensions can impact renewable energy deployment and electric sector CO2 emissions February 22, 2016 The Energy Department's National Renewable Energy Laboratory (NREL) today released new analysis exploring the potential impact of recently extended federal tax credits on the deployment of renewable generation technologies and related U.S. electric sector carbon dioxide (CO2) emissions. The report,

  10. Perpendicular Magnetism Unparalleled Find in Single Crystal | The Ames

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Laboratory Perpendicular Magnetism Unparalleled Find in Single Crystal Two distinct types of magnetism aligned perpendicular in a single crystal have been detailed in new measurements on single-crystal and powered samples composed of barium, potassium, manganese, and arsenic. Antiferromagnetism occurs with a checkerboard-style patterning of the total atomic magnetic moments due to the spins of the localized electrons of the manganese atoms (known as 'local-moment magnetism'). Aligning

  11. FOR THE SUPPLEMENTAL ENVIRONMENTAL ASSESSMENT/FINDING OF NO

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    2 FOR THE SUPPLEMENTAL ENVIRONMENTAL ASSESSMENT/FINDING OF NO SIGNIFICANT IMPACT FOR THE UNIVERSITY OF MAINE'S DEEPWATER OFFSHORE FLOATING WIND TURBINE TESTING AND DEMONSTRATION PROJECT CASTINE, MAINE DOE/EA-1792-S1 US Department of Energy Office of Energy Efficiency and Renewable Energy Golden, Colorado November 2014 Supplement Analysis - 2 i November 20, 2014 DOE/EA-1792-S1 CONTENTS 1.0 Background

  12. Finding Solutions to Solar's Soft Cost Dilemma | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Solutions to Solar's Soft Cost Dilemma Finding Solutions to Solar's Soft Cost Dilemma January 8, 2013 - 1:22pm Addthis Brian Webster installs rooftop solar panels on a home in Englewood, Colorado. The Energy Department is working to streamline rooftop solar installations so that its faster, easier and cheaper for Americans to go solar. | Photo courtesy of Dennis Schroeder, NREL. Brian Webster installs rooftop solar panels on a home in Englewood, Colorado. The Energy Department is working to

  13. Researchers find potential key for unlocking biomass energy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Unlocking biomass energy Researchers find potential key for unlocking biomass energy Potential pretreatment method that can make plant cellulose five times more digestible by enzymes that convert it into ethanol, a useful biofuel. July 20, 2011 Los Alamos National Laboratory sits on top of a once-remote mesa in northern New Mexico with the Jemez mountains as a backdrop to research and innovation covering multi-disciplines from bioscience, sustainable energy sources, to plasma physics and new

  14. Summer interns present research findings in poster session | Princeton

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Plasma Physics Lab Summer interns present research findings in poster session By Raphael Rosen August 31, 2015 Tweet Widget Google Plus One Share on Facebook New Jersey Assemblywoman Donna Simon Talks With SULI Intern Cara Bagley (Photo by Elle Starkman) New Jersey Assemblywoman Donna Simon Talks With SULI Intern Cara Bagley Gallery: Physicist Charles Skinner and Intern Amanda Lewis (Photo by Elle Starkman) Physicist Charles Skinner and Intern Amanda Lewis Interns Matthew Lotocki, Zack

  15. NREL Scientists Find Key Function for Ferredoxins in Algae Hydrogen

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Production - News Releases | NREL Scientists Find Key Function for Ferredoxins in Algae Hydrogen Production Two of six iron-rich proteins shown to have role in algae metabolism; discovery could lead to enhanced hydrogen production February 10, 2014 Scientists at the Energy Department's National Renewable Energy Laboratory have demonstrated that just two of six iron-sulfur-containing ferredoxins in a representative species of algae promote electron transfers to and from hydrogenases. The

  16. Livermore research finds that climate models overestimate rainfall

    National Nuclear Security Administration (NNSA)

    increases | National Nuclear Security Administration research finds that climate models overestimate rainfall increases Monday, December 21, 2015 - 12:00am Researchers at Lawrence Livermore National Laboratory and collaborators have found that most climate models overestimate the increase in global precipitation due to climate change. Specifically, the team looked at 25 models and found they underestimate the increase in absorption of sunlight by water vapor as the atmosphere becomes

  17. EA-1815: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    5: Diamond Green Diesel Facility in Norco, LA EA-1795: Diamond Green Diesel Facility in Norco, LA April 1, 2011 EA-1795: Final Environmental Assessment Loan Guarantee to Diamond Green Diesel, LLC for Construction of the Diamond Green Diesel Facility in Norco, Louisiana April 18, 2011 EA-1795: Finding of No Significant Impact Loan Guarantee to Diamond Green Diesel for Construction of the Diamond Green Diesel Facility in Norco, Louisiana

    Final Environmental Assessment EA-1795: Final

  18. Exploring Plasma Science Advances from Fusion Findings to Astrophysical

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Achievements | Princeton Plasma Physics Lab Exploring Plasma Science Advances from Fusion Findings to Astrophysical Achievements By John Greenwald December 4, 2012 Tweet Widget Google Plus One Share on Facebook The latest advances in plasma physics were the focus of more than 1,000 scientists from around the world who gathered in Providence, R.I., from Oct. 29 through Nov. 2 for the 54th Annual Meeting of the American Physical Society's Division of Plasma Physics (APS-DPP). Papers, posters

  19. ORISE: Study finds foreign doctorate recipients' stay rates remain high

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Study finds rate of foreign doctorate recipients staying in the United States remains high No evidence that visa restrictions are reducing stay rates, according to report FOR IMMEDIATE RELEASE Jan. 18, 2012 FY12-12 OAK RIDGE, Tenn.-The number of foreign students pursuing science and engineering doctorates in the United States continues to trend upward, and the rates at which they remain in the United States to work after graduation are at or near the highest levels observed for the various

  20. Transportation Energy Futures: Project Overview and Findings (Presentation)

    SciTech Connect (OSTI)

    Not Available

    2013-03-01

    The U.S. Department of Energy-sponsored Transportation Energy Futures (TEF) project examines how combining multiple strategies could reduce both GHG emissions and petroleum use by 80%. The project's primary objective was to help inform domestic decisions about transportation energy strategies, priorities, and investments, with an emphasis on previously underexplored opportunities related to energy efficiency and renewable energy in light-duty vehicles, non-light-duty vehicles, fuels, and transportation demand. This PowerPoint provides an overview of the project and its findings.

  1. Research Finds Vitamin D Deficiency Affects Bone Quality

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Research Finds Vitamin D Deficiency Affects Bone Quality Print Vitamin D deficiency is a widespread medical condition that plays a major role in human bone health. Scientists know that a lack of vitamin D can cause bone diseases such as rickets and osteomalacia. Now a team of researchers working at the ALS has also found that vitamin D deficiency plays a significant role in the bone-aging process. Low levels of vitamin D, the "sunshine vitamin," have been previously linked to the

  2. Graph Coarsening for Path Finding in Cybersecurity Graphs

    SciTech Connect (OSTI)

    Hogan, Emilie A.; Johnson, John R.; Halappanavar, Mahantesh

    2013-01-01

    n the pass-the-hash attack, hackers repeatedly steal password hashes and move through a computer network with the goal of reaching a computer with high level administrative privileges. In this paper we apply graph coarsening in network graphs for the purpose of detecting hackers using this attack or assessing the risk level of the network's current state. We repeatedly take graph minors, which preserve the existence of paths in the graph, and take powers of the adjacency matrix to count the paths. This allows us to detect the existence of paths as well as find paths that have high risk of being used by adversaries.

  3. Research Finds Vitamin D Deficiency Affects Bone Quality

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Research Finds Vitamin D Deficiency Affects Bone Quality Print Vitamin D deficiency is a widespread medical condition that plays a major role in human bone health. Scientists know that a lack of vitamin D can cause bone diseases such as rickets and osteomalacia. Now a team of researchers working at the ALS has also found that vitamin D deficiency plays a significant role in the bone-aging process. Low levels of vitamin D, the "sunshine vitamin," have been previously linked to the

  4. Research Finds Vitamin D Deficiency Affects Bone Quality

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Research Finds Vitamin D Deficiency Affects Bone Quality Print Vitamin D deficiency is a widespread medical condition that plays a major role in human bone health. Scientists know that a lack of vitamin D can cause bone diseases such as rickets and osteomalacia. Now a team of researchers working at the ALS has also found that vitamin D deficiency plays a significant role in the bone-aging process. Low levels of vitamin D, the "sunshine vitamin," have been previously linked to the

  5. Research Finds Vitamin D Deficiency Affects Bone Quality

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Research Finds Vitamin D Deficiency Affects Bone Quality Print Vitamin D deficiency is a widespread medical condition that plays a major role in human bone health. Scientists know that a lack of vitamin D can cause bone diseases such as rickets and osteomalacia. Now a team of researchers working at the ALS has also found that vitamin D deficiency plays a significant role in the bone-aging process. Low levels of vitamin D, the "sunshine vitamin," have been previously linked to the

  6. Research Finds Vitamin D Deficiency Affects Bone Quality

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Research Finds Vitamin D Deficiency Affects Bone Quality Print Vitamin D deficiency is a widespread medical condition that plays a major role in human bone health. Scientists know that a lack of vitamin D can cause bone diseases such as rickets and osteomalacia. Now a team of researchers working at the ALS has also found that vitamin D deficiency plays a significant role in the bone-aging process. Low levels of vitamin D, the "sunshine vitamin," have been previously linked to the

  7. Scientist finds new way to predict heat layer troublemaker | Princeton

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Plasma Physics Lab Scientist finds new way to predict heat layer troublemaker By John Greenwald August 27, 2012 Tweet Widget Google Plus One Share on Facebook Researchers at a recent worldwide conference on fusion power have confirmed the surprising accuracy of a new model for predicting the size of a key barrier to fusion that a top scientist at the U.S. Department of Energy's Princeton Plasma Physics Laboratory (PPPL) has developed. The model could serve as a starting point for overcoming

  8. Finding New Ways to Foster Clean Energy Partnerships - Continuum Magazine

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    | NREL photo of three men wearing hard hats standing in front of a wind dynamometer with DOE, NREL, and GE logos printed on the side. While touring NREL's National Wind Technology Center (NWTC) with NREL Director Dan Arvizu and NWTC Director Fort Felker in July, U.S. Department of Energy Assistant Secretary David Danielson discussed the importance of working with industry partners to enhance the integration of wind into our nation's energy mix. Photo by Pat Corkery, NREL Finding New Ways to

  9. Unique Bioreactor Finds Algae's Sweet Spot - News Feature | NREL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Unique Bioreactor Finds Algae's Sweet Spot February 18, 2014 Close-up photo of a vial of green algae. Enlarge image Aeration helps algae grow and helps replicate real-life conditions in the Simulated Algal Growth Environment (SAGE) reactor at NREL. The reactor controls light and temperature, helping scientists determine not just what strain will grow the best, but where in the United States it may do so. Ideal strains can be harvested for their lipids, proteins, and sugars for use in biofuels.

  10. Microsoft Word - fact sheet alternatives and key findings 090214.docx

    National Nuclear Security Administration (NNSA)

    Alternatives and Key Findings of the Draft SEIS ALTERNATIVES The Draft SEIS evaluates a range of reactor site and tritium production capacity alternatives. Seven alternatives are analyzed in the Draft SEIS:  Alternative 1 Watts Bar site only (2,500 TPBARs maximum). This is the preferred alternative.  Alternative 2 Sequoyah site only (2,500 TPBARs maximum).  Alternative 3 Both Watts Bar and Sequoyah sites (2,500 TPBARs maximum).  Alternative 4 Watts Bar site only (5,000 TPBARs

  11. Role of a Transcriptional Regulator in Programmed Cell Death and Plant Development

    SciTech Connect (OSTI)

    Julie M. Stone

    2008-09-13

    The long-term goal of this research is to understand the role(s) and molecular mechanisms of programmed cell death (PCD) in the controlling plant growth, development and responses to biotic and abiotic stress. We developed a genetic selection scheme to identify A. thaliana FB1-resistant (fbr) mutants as a way to find genes involved in PCD (Stone et al., 2000; Stone et al., 2005; Khan and Stone, 2008). The disrupted gene in fbr6 (AtSPL14) responsible for the FB1-insensitivity and plant architecture phenotypes encodes a plant-specific SBP DNA-binding domain transcriptional regulator (Stone et al., 2005; Liang et al., 2008). This research plan is designed to fill gaps in the knowledge about the role of SPL14 in plant growth and development. The work is being guided by three objectives aimed at determining the pathways in which SPL14 functions to modulate PCD and/or plant development: (1) determine how SPL14 functions in plant development, (2) identify target genes that are directly regulated by SPL14, and (3) identify SPL14 modifications and interacting proteins. We made significant progress during the funding period. Briefly, some major accomplishments are highlighted below: (1) To identify potential AtSPL14 target genes, we identified a consensus DNA binding site for the AtSPL14 SBP DNA-binding domain using systematic evolution of ligands by exponential selection (SELEX) and site-directed mutagenesis (Liang et al., 2008). This consensus binding site was used to analyze Affymetrix microarray gene expression data obtained from wild-type and fbr6 mutant plants to find possible AtSPL14-regulated genes. These candidate AtSPL14-regulated genes are providing new information on the molecular mechanisms linking plant PCD and plant development through modulation of the 26S proteasome. (2) Transgenic plants expressing epitope-tagged versions of AtSPL14 are being used to confirm the AtSPL14 targets (by ChIP-PCR) and further dissect the molecular interactions (Nazarenus, Liang and Stone, in preparation) (3) Double mutants generated between fbr6 and various accelerated cell death (acd) mutants indicate that sphingolipid metabolism is influenced by AtSPL14 and sphingolipidomics profiling supports this conclusion (Lin, Markham and Stone, in preparation). (4) A new set of phenotypes have been uncovered in the original fbr6-1 mutant, including a short-root phenotype related to auxin signaling and altered photosynthetic parameters related to stomatal density and conductance (Lin and Stone, in preparation; Lin, Madhavan and Stone, in preparation). Additional AtSPL14-related mutants and transgenic plants have been generated to effectively dissect the functions of AtSPL14, including a dominant negative fbr6-2 allele and transgenic plants overexpressing FBR6/AtSPL14 that display an accelerated cell death (acd) phenotype.

  12. Finding Text Information in the Ocean of Electronic Documents

    SciTech Connect (OSTI)

    Medvick, Patricia A.; Calapristi, Augustin J.

    2003-02-05

    Information management in natural resources has become an overwhelming task. A massive amount of electronic documents and data is now available for creating informed decisions. The problem is finding the relevant information to support the decision-making process. Determining gaps in knowledge in order to propose new studies or to determine which proposals to fund for maximum potential is a time-consuming and difficult task. Additionally, available data stores are increasing in complexity; they now may include not only text and numerical data, but also images, sounds, and video recordings. Information visualization specialists at Pacific Northwest National Laboratory (PNNL) have software tools for exploring electronic data stores and for discovering and exploiting relationships within data sets. These provide capabilities for unstructured text explorations, the use of data signatures (a compact format for the essence of a set of scientific data) for visualization (Wong et al 2000), visualizations for multiple query results (Havre et al. 2001), and others (http://www.pnl.gov/infoviz ). We will focus on IN-SPIRE, a MS Windows vision of PNNL’s SPIRE (Spatial Paradigm for Information Retrieval and Exploration). IN-SPIRE was developed to assist information analysts find and discover information in huge masses of text documents.

  13. Chk2 regulates transcription-independent p53-mediated apoptosis in response to DNA damage

    SciTech Connect (OSTI)

    Chen Chen [Department of Geriatric Research, National Institute for Longevity Sciences, National Center for Geriatrics and Gerontology, Obu, Aichi 474-8522 (Japan); Shimizu, Shigeomi [Department of Post-Genomics Diseases, Osaka University Medical School, Suita, Osaka 565-0871 (Japan); Tsujimoto, Yoshihide [Department of Post-Genomics Diseases, Osaka University Medical School, Suita, Osaka 565-0871 (Japan); Motoyama, Noboru [Department of Geriatric Research, National Institute for Longevity Sciences, National Center for Geriatrics and Gerontology, Obu, Aichi 474-8522 (Japan)]. E-mail: motoyama@nils.go.jp

    2005-07-29

    The tumor suppressor protein p53 plays a central role in the induction of apoptosis in response to genotoxic stress. The protein kinase Chk2 is an important regulator of p53 function in mammalian cells exposed to ionizing radiation (IR). Cells derived from Chk2-deficient mice are resistant to the induction of apoptosis by IR, and this resistance has been thought to be a result of the defective transcriptional activation of p53 target genes. It was recently shown, however, that p53 itself and histone H1.2 translocate to mitochondria and thereby induces apoptosis in a transcription-independent manner in response to IR. We have now examined whether Chk2 also regulates the transcription-independent induction of apoptosis by p53 and histone H1.2. The reduced ability of IR to induce p53 stabilization in Chk2-deficient thymocytes was associated with a marked impairment of p53 and histone H1 translocation to mitochondria. These results suggest that Chk2 regulates the transcription-independent mechanism of p53-mediated apoptosis by inducing stabilization of p53 in response to IR.

  14. Parallel halo finding in N-body cosmology simulations

    SciTech Connect (OSTI)

    Pfitzner, D.W.; Salmon, J.K.

    1996-12-31

    Cosmological N-body simulations on parallel computers produce large datasets - about five hundred Megabytes at a single output time, or tens of Gigabytes over the course of a simulation. These large datasets require further analysis before they can be compared to astronomical observations. We have implemented two methods for performing halo finding, a key part of the knowledge discovery process, on parallel machines. One of these is a parallel implementation of the friends of friends (FOF) algorithm, widely used in the field of N-body cosmology. The new isodensity (ID) method has been developed to overcome some of the shortcomings of FOR Both have been implemented on a variety of computer systems, and successfully used to extract halos from simulations with up to 256{sup 3} (or about 16.8 million) particles, which axe among the largest N-body cosmology simulations in existence.

  15. Utility of Social Modeling for Proliferation Assessment - Preliminary Findings

    SciTech Connect (OSTI)

    Coles, Garill A.; Gastelum, Zoe N.; Brothers, Alan J.; Thompson, Sandra E.

    2009-07-16

    Often the methodologies for assessing proliferation risk are focused around the inherent vulnerability of nuclear energy systems and associated safeguards. For example an accepted approach involves ways to measure the intrinsic and extrinsic barriers to potential proliferation. This paper describes preliminary investigation into non-traditional use of social and cultural information to improve proliferation assessment and advance the approach to assessing nuclear material diversion. Proliferation resistance assessment, safeguard assessments and related studies typically create technical information about the vulnerability of a nuclear energy system to diversion of nuclear material. The purpose of this research project is to find ways to integrate social information with technical information by explicitly considering the role of culture, groups and/or individuals to factors that impact the possibility of proliferation. When final, this work is expected to describe and demonstrate the utility of social science modeling in proliferation and proliferation risk assessments.

  16. Histone H4 Lys 20 methyltransferase SET8 promotes androgen receptor-mediated transcription activation in prostate cancer

    SciTech Connect (OSTI)

    Yao, Lushuai; Li, Yanyan; Du, Fengxia; Han, Xiao; Li, Xiaohua; Niu, Yuanjie; Ren, Shancheng; Sun, Yingli

    2014-07-18

    Highlights: • Dihydrotestosterone stimulates H4K20me1 enrichment at the PSA promoter. • SET8 promotes AR-mediated transcription activation. • SET8 interacts with AR and promotes cell proliferation. - Abstract: Histone methylation status in different lysine residues has an important role in transcription regulation. The effect of H4K20 monomethylation (H4K20me1) on androgen receptor (AR)-mediated gene transcription remains unclear. Here we show that AR agonist stimulates the enrichment of H4K20me1 and SET8 at the promoter of AR target gene PSA in an AR dependent manner. Furthermore, SET8 is crucial for the transcription activation of PSA. Co-immunoprecipitation analyses demonstrate that SET8 interacts with AR. Therefore, we conclude that SET8 is involved in AR-mediated transcription activation, possibly through its interaction with AR and H4K20me1 modification.

  17. Low concentrations of bisphenol a suppress thyroid hormone receptor transcription through a nongenomic mechanism

    SciTech Connect (OSTI)

    Sheng, Zhi-Guo; Tang, Yuan; Liu, Yu-Xiang; Yuan, Ye; Zhao, Bao-Quan; Chao, Xi-Juan; Zhu, Ben-Zhan

    2012-02-15

    Bisphenol (BPA) is one of the highest-volume chemicals produced worldwide, and human exposure to BPA is thought to be ubiquitous. Various rodent and in vitro studies have shown that thyroid hormone (TH) function can be impaired by BPA. However, it is still unknown if low concentrations of BPA can suppress the thyroid hormone receptor (TR) transcription. The present study aims to investigate the possible suppressing effects of low concentrations of BPA on TR transcription and the involved mechanism(s) in CV-1 cells derived from cercopithecus aethiops monkey kidneys. Using gene reporter assays, BPA at concentrations as low as 10{sup ?9} M suppresses TR or steroid receptor coactivator-1(SRC-1)-enhanced TR transcription, but not reducing TR/SRC-1 interaction in mammalian two-hybrid and glutathione S-transferase pull-down studies. It has been further shown that both nuclear receptor co-repressor (N-CoR) and silencing mediator for retinoid and thyroid hormone receptors (SMRT) are recruited to the TR-?1 by BPA in the presence of physiologic concentrations of T3 or T4. However, the overexpression of ?3 integrin or c-Src significantly reduces BPA-induced recruitment of N-CoR/SMRT to TR or suppression of TR transcription. Furthermore, BPA inhibits the T3/T4-mediated interassociation of the ?3 integrin/c-Src/MAPK/TR-?1 pathways by the co-immunoprecipitation. These results indicate that low concentrations of BPA suppress the TR transcription by disrupting physiologic concentrations of T3/T4-mediated ?3 integrin/c-Src/MAPK/TR-?1 pathways, followed by recruiting N-CoR/SMRT to TR-?1, providing a novel insight regarding the TH disruption effects of low concentration BPA. -- Highlights: ? Environmentally relevant concentrations of BPA suppress TR transcription. ? BPA recruits the N-CoR/SMRT to TR under the physiologic concentrations of T3/T4. ? BPA disrupts T3/T4-mediated ?3 integrin/c-Src/MAPK/TR-?1 pathways.

  18. Energy economists unite. [International Association of Energy Economists findings

    SciTech Connect (OSTI)

    Not Available

    1983-08-10

    Three years after the Arab Oil Embargo, the International Association of Energy Economists (IAEE) was organized. A non-profit organization operated through a council of 15 elected and appointed members, IAEE has already attracted some 1400 individual members from around the world as well as numerous affiliates among national energy organizations. In this issue, Energy Detente reports various IAEE findings, including: (1) a possible 1% growth during 1983 in total energy demand in capitalist countries to be satisfied by coal, nuclear, gas, and hydro supplies, not petroleum; petroleum is expected to figure in the expected 4% growth in energy demand during 1984; (2) usable commercial inventory of petroleum, having risen to 27 days of forward demand by the second quarter of 1981, now sits at 10 days; (3) the current cushion between oil supply and demand, now 15-million barrels per day (b/d) on the side of surplus production capacity during the first quarter of 1983, is exerting downward pressure on prices; and (4) possibly only half the effect of conservation provoked by the second oil-price shock has yet been manifested. If prices fall below US $25 bbl, the second half might be deferred indefinitely, and some enhanced-recovery and frontier-recovery projects would become uneconomic. Also, with OPEC prices holding through the summer of 1983, there is a good chance prices could stabilize for the next several years. This issue presents the Energy Detente fuel price/tax series and the principal industrial fuel prices for August 1983 for countries of the Eastern Hemisphere.

  19. Molecular genetic analysis of activation-tagged transcription factors thought to be involved in photomorphogenesis

    SciTech Connect (OSTI)

    Neff, Michael M.

    2011-06-23

    This is a final report for Department of Energy Grant No. DE-FG02-08ER15927 entitled “Molecular Genetic Analysis of Activation-Tagged Transcription Factors Thought to be Involved in Photomorphogenesis”. Based on our preliminary photobiological and genetic analysis of the sob1-D mutant, we hypothesized that OBP3 is a transcription factor involved in both phytochrome and cryptochrome-mediated signal transduction. In addition, we hypothesized that OBP3 is involved in auxin signaling and root development. Based on our preliminary photobiological and genetic analysis of the sob2-D mutant, we also hypothesized that a related gene, LEP, is involved in hormone signaling and seedling development.

  20. Fungal-specific transcription factor AbPf2 activates pathogenicity in Alternaria brassicicola

    SciTech Connect (OSTI)

    Cho, Yangrae; Ohm, Robin A.; Grigoriev, Igor V.; Srivastava, Akhil

    2012-12-03

    Alternaria brassicicola is a successful saprophyte and necrotrophic plant pathogen. To identify molecular determinants of pathogenicity, we created non-pathogenic mutants of a transcription factor-encoding gene, AbPf2. The frequency and timing of germination and appressorium formation on host plants were similar between the non-pathogenic abpf2 mutants and wild-type A. brassicicola. The mutants were also similar in vitro to wild-type A. brassicicola in terms of vegetative growth, conidium production, and responses to a phytoalexin, reactive oxygen species and osmolites. The hyphae of the mutants grew slowly but did not cause disease symptoms on the surface of host plants. Transcripts of the AbPf2 gene increased exponentially soon after wild-type conidia contacted their host plants . A small amount of AbPf2 protein, as monitored using GFP fusions, was present in young, mature conidia. The protein level decreased during saprophytic growth, but increased and was located primarily in fungal nuclei during pathogenesis. Levels of the proteins and transcripts sharply decreased following colonization of host tissues beyond the initial infection site. When expression of the transcription factor was induced in the wild-type during early pathogenesis, 106 fungal genes were also induced in the wild-type but not in the abpf2 mutants. Notably, 33 of the 106 genes encoded secreted proteins, including eight putative effector proteins. Plants inoculated with abpf2 mutants expressed higher levels of genes associated with photosynthesis, the pentose phosphate pathway and primary metabolism, but lower levels of defense-related genes. Our results suggest that AbPf2 is an important regulator of pathogenesis, but does not affect other cellular processes in A. brassicicola.

  1. CITED2 modulates estrogen receptor transcriptional activity in breast cancer cells

    SciTech Connect (OSTI)

    Lau, Wen Min; Doucet, Michele; Huang, David; Weber, Kristy L.; Kominsky, Scott L.

    2013-07-26

    Highlights: The effects of elevated CITED2 on ER function in breast cancer cells are examined. CITED2 enhances cell growth in the absence of estrogen and presence of tamoxifen. CITED2 functions as a transcriptional co-activator of ER in breast cancer cells. -- Abstract: Cbp/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain 2 (CITED2) is a member of the CITED family of non-DNA binding transcriptional co-activators of the p300/CBP-mediated transcription complex. Previously, we identified CITED2 as being overexpressed in human breast tumors relative to normal mammary epithelium. Upon further investigation within the estrogen receptor (ER)-positive subset of these breast tumor samples, we found that CITED2 mRNA expression was elevated in those associated with poor survival. In light of this observation, we investigated the effect of elevated CITED2 levels on ER function. While ectopic overexpression of CITED2 in three ER-positive breast cancer cell lines (MCF-7, T47D, and CAMA-1) did not alter cell proliferation in complete media, growth was markedly enhanced in the absence of exogenous estrogen. Correspondingly, cells overexpressing CITED2 demonstrated reduced sensitivity to the growth inhibitory effects of the selective estrogen receptor modulator, 4-hydroxytamoxifen. Subsequent studies revealed that basal ER transcriptional activity was elevated in CITED2-overexpressing cells and was further increased upon the addition of estrogen. Similarly, basal and estrogen-induced expression of the ER-regulated genes trefoil factor 1 (TFF1) and progesterone receptor (PGR) was higher in cells overexpressing CITED2. Concordant with this observation, ChIP analysis revealed higher basal levels of CITED2 localized to the TFF-1 and PGR promoters in cells with ectopic overexpression of CITED2, and these levels were elevated further in response to estrogen stimulation. Taken together, these data indicate that CITED2 functions as a transcriptional co-activator of ER in breast cancer cells and that its increased expression in tumors may result in estrogen-independent ER activation, thereby reducing estrogen dependence and response to anti-estrogen therapy.

  2. Sex-related differences in murine hepatic transcriptional and proteomic responses to TCDD

    SciTech Connect (OSTI)

    Prokopec, Stephenie D.; Watson, John D.; Lee, Jamie; Pohjanvirta, Raimo; Boutros, Paul C.

    2015-04-15

    2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is an environmental contaminant that produces myriad toxicities in most mammals. In rodents alone, there is a huge divergence in the toxicological response across species, as well as among different strains within a species. But there are also significant differences between males and females animals of a single strain. These differences are inconsistent across model systems: the severity of toxicity is greater in female rats than males, while male mice and guinea pigs are more sensitive than females. Because the specific events that underlie this difference remain unclear, we characterized the hepatic transcriptional response of adult male and female C57BL/6 mice to 500 μg/kg TCDD at multiple time-points. The transcriptional profile diverged significantly between the sexes. Female mice demonstrated a large number of altered transcripts as early as 6 h following treatment, suggesting a large primary response. Conversely, male animals showed the greatest TCDD-mediated response 144 h following exposure, potentially implicating significant secondary responses. Nr1i3 was statistically significantly induced at all time-points in the sensitive male animals. This mRNA encodes the constitutive androstane receptor (CAR), a transcription factor involved in the regulation of xenobiotic metabolism, lipid metabolism, cell cycle and apoptosis. Surprisingly though, changes at the protein level (aside from the positive control, CYP1A1) were modest, with only FMO3 showing clear induction, and no genes with sex-differences. Thus, while male and female mice show transcriptional differences in their response to TCDD, their association with TCDD-induced toxicities remains unclear. - Highlights: • Differences exist between the toxicity phenotypes to TCDD in male and female mice. • TCDD-mediated transcriptomic differences were identified between the sexes. • Resistant female mice displayed a large, early-onset, transcriptomic response. • Sensitive male mice displayed a large, late-onset, transcriptomic response. • Fmo2, Fmo3 and Nr1i3 were induced across the time-course in only male mice.

  3. Identification and overexpression of a knotted1-like transcription factor in switchgrass (Panicum virgatum L.) for lignocellulosic feedstock improvement

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Wuddineh, Wegi A.; Mazarei, Mitra; Zhang, Ji -Yi; Turner, Geoffrey B.; Sykes, Robert W.; Decker, Stephen R.; Davis, Mark F.; Udvardi, Michael K.; C. Neal Stewart, Jr.

    2016-04-28

    High biomass production and wide adaptation has made switchgrass (Panicum virgatum L.) an important candidate lignocellulosic bioenergy crop. One major limitation of this and other lignocellulosic feedstocks is the recalcitrance of complex carbohydrates to hydrolysis for conversion to biofuels. Lignin is the major contributor to recalcitrance as it limits the accessibility of cell wall carbohydrates to enzymatic breakdown into fermentable sugars. Therefore, genetic manipulation of the lignin biosynthesis pathway is one strategy to reduce recalcitrance. Here, we identified a switchgrass Knotted1 transcription factor, PvKN1, with the aim of genetically engineering switchgrass for reduced biomass recalcitrance for biofuel production. Gene expressionmore » of the endogenous PvKN1 gene was observed to be highest in young inflorescences and stems. Ectopic overexpression of PvKN1 in switchgrass altered growth, especially in early developmental stages. Transgenic lines had reduced expression of most lignin biosynthetic genes accompanied by a reduction in lignin content suggesting the involvement of PvKN1 in the broad regulation of the lignin biosynthesis pathway. Moreover, the reduced expression of the Gibberellin 20-oxidase (GA20ox) gene in tandem with the increased expression of Gibberellin 2-oxidase (GA2ox) genes in transgenic PvKN1 lines suggest that PvKN1 may exert regulatory effects via modulation of GA signaling. Furthermore, overexpression of PvKN1 altered the expression of cellulose and hemicellulose biosynthetic genes and increased sugar release efficiency in transgenic lines. Our findings demonstrated that switchgrass PvKN1 is a putative ortholog of maize KN1 that is linked to plant lignification and cell wall and development traits as a major regulatory gene. Therefore, targeted overexpression of PvKN1 in bioenergy feedstocks may provide one feasible strategy for reducing biomass recalcitrance and simultaneously improving plant growth characteristics.« less

  4. The oncoprotein HBXIP upregulates PDGFB via activating transcription factor Sp1 to promote the proliferation of breast cancer cells

    SciTech Connect (OSTI)

    Zhang, Yingyi; Zhao, Yu; Li, Leilei; Shen, Yu; Cai, Xiaoli; Zhang, Xiaodong; Ye, Lihong

    2013-05-03

    Highlights: HBXIP is able to upregulate the expression of PDGFB in breast cancer cells. HBXIP serves as a coactivator of activating transcription factor Sp1. HBXIP stimulates the PDGFB promoter via activating transcription factor Sp1. HBXIP promotes the proliferation of breast cancer cell via upregulating PDGFB. -- Abstract: We have reported that the oncoprotein hepatitis B virus X-interacting protein (HBXIP) acts as a novel transcriptional coactivator to promote proliferation and migration of breast cancer cells. Previously, we showed that HBXIP was able to activate nuclear factor-?B (NF-?B) in breast cancer cells. As an oncogene, the platelet-derived growth factor beta polypeptide (PDGFB) plays crucial roles in carcinogenesis. In the present study, we found that both HBXIP and PDGFB were highly expressed in breast cancer cell lines. Interestingly, HBXIP was able to increase transcriptional activity of NF-?B through PDGFB, suggesting that HBXIP is associated with PDGFB in the cells. Moreover, HBXIP was able to upregulate PDGFB at the levels of mRNA, protein and promoter in the cells. Then, we identified that HBXIP stimulated the promoter of PDGFB through activating transcription factor Sp1. In function, HBXIP enhanced the proliferation of breast cancer cells through PDGFB in vitro. Thus, we conclude that HBXIP upregulates PDGFB via activating transcription factor Sp1 to promote proliferation of breast cancer cells.

  5. Light-up and FRET aptamer reporters; evaluating their applications for imaging transcription in eukaryotic cells

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Ilgu, Muslum; Ray, Judhajeet; Bendickson, Lee; Wang, Tianjiao; Geraskin, Ivan M.; Kraus, George A.; Nilsen-Hamilton, Marit

    2015-12-17

    The regulation of RNA transcription is central to cellular function. Changes in gene expression drive differentiation and cellular responses to events such as injury. RNA trafficking can also have a large impact on protein expression and its localization. Thus, the ability to image RNA transcription and trafficking in real time and in living cells is a worthwhile goal that has been difficult to achieve. The availability of “light-up” aptamers that cause an increase in fluorescence of their ligands when bound by the aptamer have shown promise for reporting on RNA production and localization in vivo. Here we have investigated twomore » light-up aptamers (the malachite green aptamer and the Spinach aptamers) for their suitabilities as reporters of RNA expression in vivo using two eukaryotic cell types, yeast and mammalian. Our analysis focused on the aptamer ligands, their contributions to background noise, and the impact of tandem aptamer strings on signal strength and ligand affinity. Whereas the background fluorescence is very low in vitro, this is not always true for cell imaging. Our results suggest the need for caution in using light-up aptamers as reporters for imaging RNA. In particular, images should be collected and analyzed by operators blinded to the sample identities. The appropriate control condition of ligand with the cells in the absence of aptamer expression must be included in each experiment. This control condition establishes that the specific interaction of ligand with aptamer, rather than nonspecific interactions with unknown cell elements, is responsible for the observed fluorescent signals. As a result, high background signals due to nonspecific interactions of aptamer ligands with cell components can be minimized by using IMAGEtags (Intracellular Multiaptamer GEnetic tags), which signal by FRET and are promising RNA reporters for imaging transcription.« less

  6. Inhibition of hepatitis B virus (HBV) by LNA-mediated nuclear interference with HBV DNA transcription

    SciTech Connect (OSTI)

    Sun, Zhen; Department of Biochemistry and Molecular Biology, Program in Molecular Cell Biology, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058 ; Xiang, Wenqing; Guo, Yajuan; Chen, Zhi; Liu, Wei; Lu, Daru

    2011-06-10

    Highlights: {yields} LNA-modified oligonucleotides can pass through the plasma membrane of cultured cells even without using transfection machinery. {yields} LNA-modified oligonucleotides passed efficiently across the cell membrane, and lipid-coating facilitated translocation from the cytoplasm to the nucleus. {yields} LNA-oligonucleotide designed to target nuclear HBV DNA efficiently suppresses HBV replication and transcription in cultured hepatic cells. -- Abstract: Silencing target genes with small regulatory RNAs is widely used to investigate gene function and therapeutic drug development. Recently, triplex-based approaches have provided another attractive means to achieve targeted gene regulation and gene manipulation at the molecular and cellular levels. Nuclear entry of oligonucleotides and enhancement of their affinity to the DNA targets are key points of such approaches. In this study, we developed lipid-based transport of a locked-nucleic-acid (LNA)-modified oligonucleotide for hepatitis B virus (HBV) DNA interference in human hepatocytes expressing HBV genomic DNA. In these cells, the LNA-modified oligonucleotides passed efficiently across the cell membrane, and lipid-coating facilitated translocation from the cytoplasm to the nucleus. The oligonucleotide specifically targeting HBV DNA clearly interfered with HBV DNA transcription as shown by a block in pregenomic RNA (pgRNA) production. The HBV DNA-targeted oligonucleotide suppressed HBV DNA replication and HBV protein production more efficiently than small interfering RNAs directed to the pgRNA. These results demonstrate that fusion with lipid can carry LNA-modified oligonucleotides to the nucleus where they regulate gene expression. Interfering with HBV DNA transcription by LNA-modified oligonucleotides has strong potential as a new strategy for HBV inhibition.

  7. ECRbase: Database of Evolutionary Conserved Regions, Promoters, and Transcription Factor Binding Sites in Vertebrate Genomes

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Loots, Gabriela G. [LLNL; Ovcharenko, I. [LLNL

    Evolutionary conservation of DNA sequences provides a tool for the identification of functional elements in genomes. This database of evolutionary conserved regions (ECRs) in vertebrate genomes features a database of syntenic blocks that recapitulate the evolution of rearrangements in vertebrates and a comprehensive collection of promoters in all vertebrate genomes generated using multiple sources of gene annotation. The database also contains a collection of annotated transcription factor binding sites (TFBSs) in evolutionary conserved and promoter elements. ECRbase currently includes human, rhesus macaque, dog, opossum, rat, mouse, chicken, frog, zebrafish, and fugu genomes. (taken from paper in Journal: Bioinformatics, November 7, 2006, pp. 122-124

  8. Structurally distinct polycyclic aromatic hydrocarbons induce differential transcriptional responses in developing zebrafish

    SciTech Connect (OSTI)

    Goodale, Britton C.; Tilton, Susan C.; Corvi, Margaret M.; Wilson, Glenn R.; Janszen, Derek B.; Anderson, Kim A.; Waters, Katrina M.; Tanguay, Robert L.

    2013-11-01

    Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous in the environment as components of fossil fuels and by-products of combustion. These multi-ring chemicals differentially activate the aryl hydrocarbon receptor (AHR) in a structurally dependent manner, and induce toxicity via both AHR-dependent and -independent mechanisms. PAH exposure is known to induce developmental malformations in zebrafish embryos, and recent studies have shown cardiac toxicity induced by compounds with low AHR affinity. Unraveling the potentially diverse molecular mechanisms of PAH toxicity is essential for understanding the hazard posed by complex PAH mixtures present in the environment. We analyzed transcriptional responses to PAH exposure in zebrafish embryos exposed to benz(a)anthracene (BAA), dibenzothiophene (DBT) and pyrene (PYR) at concentrations that induced developmental malformations by 120 h post-fertilization (hpf). Whole genome microarray analysis of mRNA expression at 24 and 48 hpf identified genes that were differentially regulated over time and in response to the three PAH structures. PAH body burdens were analyzed at both time points using GCMS, and demonstrated differences in PAH uptake into the embryos. This was important for discerning dose-related differences from those that represented unique molecular mechanisms. While BAA misregulated the least number of transcripts, it caused strong induction of cyp1a and other genes known to be downstream of the AHR, which were not induced by the other two PAHs. Analysis of functional roles of misregulated genes and their predicted regulatory transcription factors also distinguished the BAA response from regulatory networks disrupted by DBT and PYR exposure. These results indicate that systems approaches can be used to classify the toxicity of PAHs based on the networks perturbed following exposure, and may provide a path for unraveling the toxicity of complex PAH mixtures. - Highlights: Defined global mRNA expression changes induced by developmental exposure to PAHs Determined PAH body burdens following developmental exposure Genes uniquely induced by benz(a)anthracene included targets of the AHR and RELA Dibenzothiophene and pyrene perturbed a distinct RELA network Transcriptional networks reveal differential mechanisms of PAH toxicity.

  9. Crystal structure of enterococcus faecalis sly A-like transcriptional factor.

    SciTech Connect (OSTI)

    Wu, R.; Zhang, R.; Zagnitko, O.; Dementieva, I.; Maltsev, N.; Watson, J. D.; Laskowski, R.; Gornicki, P.; Joachimiak, A.; Univ. of Chicago; European Bioinformatics Inst.

    2003-05-30

    The crystal structure of a SlyA transcriptional regulator at 1.6 {angstrom} resolution is presented, and structural relationships between members of the MarR/SlyA family are discussed. The SlyA family, which includes SlyA, Rap, Hor, and RovA proteins, is widely distributed in bacterial and archaeal genomes. Current evidence suggests that SlyA-like factors act as repressors, activators, and modulators of gene transcription. These proteins have been shown to up-regulate the expression of molecular chaperones, acid-resistance proteins, and cytolysin, and down-regulate several biosynthetic enzymes. The structure of SlyA from Enterococcus faecalis, determined as a part of an ongoing structural genomics initiative (www.mcsg.anl.gov), revealed the same winged helix DNA-binding motif that was recently found in the MarR repressor from Escherichia coli and the MexR repressor from Pseudomonas aeruginosa, a sequence homologue of MarR. Phylogenetic analysis of the MarR/SlyA family suggests that Sly is placed between the SlyA and MarR subfamilies and shows significant sequence similarity to members of both subfamilies.

  10. mRNA Transcript Abundance during Plant Growth and the Influence of Li+ Exposure

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Duff, M. C.; Kuhne, W. W.; Halverson, N. V.; Chang, C. -S.; Kitamura, E.; Hawthorn, L.; Martinez, N. E.; Stafford, C.; Milliken, C. E.; Caldwell, E. F.; et al

    2014-10-23

    Lithium (Li) toxicity in plants is, at a minimum, a function of Li+ concentration, exposure time, species and growth conditions. Most plant studies with Li+ focus on short-term acute exposures. This study examines short- and long-term effects of Li+ exposure in Arabidopsis with Li+ uptake studies and measured shoot mRNA transcript abundance levels in treated and control plants. Stress, pathogen-response and arabinogalactan protein genes were typically more up-regulated in older (chronic, low level) Li+-treatment plants and in the much younger plants from acute high-level exposures. The gene regulation behavior of high-level Li+ resembled prior studies due to its influence on:more »inositol synthesis, 1-aminocyclopropane-1-carboxylate synthases and membrane ion transport. In contrast, chronically-exposed plants had gene regulation responses that were indicative of pathogen, cold, and heavy-metal stress, cell wall degradation, ethylene production, signal transduction, and calcium-release modulation. Acute Li+ exposure phenocopies magnesium-deficiency symptoms and is associated with elevated expression of stress response genes that could lead to consumption of metabolic and transcriptional energy reserves and the dedication of more resources to cell development. In contrast, chronic Li+ exposure increases expression signal transduction genes. The identification of new Li+-sensitive genes and a gene-based “response plan” for acute and chronic Li+ exposure are delineated.« less

  11. mRNA Transcript Abundance during Plant Growth and the Influence of Li+ Exposure

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Duff, M. C.; Kuhne, W. W.; Halverson, N. V.; Chang, C. -S.; Kitamura, E.; Hawthorn, L.; Martinez, N. E.; Stafford, C.; Milliken, C. E.; Caldwell, E. F.; et al

    2014-10-23

    Lithium (Li) toxicity in plants is, at a minimum, a function of Li+ concentration, exposure time, species and growth conditions. Most plant studies with Li+ focus on short-term acute exposures. This study examines short- and long-term effects of Li+ exposure in Arabidopsis with Li+ uptake studies and measured shoot mRNA transcript abundance levels in treated and control plants. Stress, pathogen-response and arabinogalactan protein genes were typically more up-regulated in older (chronic, low level) Li+-treatment plants and in the much younger plants from acute high-level exposures. The gene regulation behavior of high-level Li+ resembled prior studies due to its influence on:more » inositol synthesis, 1-aminocyclopropane-1-carboxylate synthases and membrane ion transport. In contrast, chronically-exposed plants had gene regulation responses that were indicative of pathogen, cold, and heavy-metal stress, cell wall degradation, ethylene production, signal transduction, and calcium-release modulation. Acute Li+ exposure phenocopies magnesium-deficiency symptoms and is associated with elevated expression of stress response genes that could lead to consumption of metabolic and transcriptional energy reserves and the dedication of more resources to cell development. In contrast, chronic Li+ exposure increases expression signal transduction genes. The identification of new Li+-sensitive genes and a gene-based “response plan” for acute and chronic Li+ exposure are delineated.« less

  12. Reprogramming of human fibroblasts to pluripotent stem cells using mRNA of four transcription factors

    SciTech Connect (OSTI)

    Yakubov, Eduard; Rechavi, Gidi; Rozenblatt, Shmuel; Givol, David

    2010-03-26

    Reprogramming of differentiated cells into induced pluripotent cells (iPS) was accomplished in 2006 by expressing four, or less, embryonic stem cell (ESC)-specific transcription factors. Due to the possible danger of DNA damage and the potential tumorigenicity associated with such DNA damage, attempts were made to minimize DNA integration by the vectors involved in this process without complete success. Here we present a method of using RNA transfection as a tool for reprogramming human fibroblasts to iPS. We used RNA synthesized in vitro from cDNA of the same reprogramming four transcription factors. After transfection of the RNA, we show intracellular expression and nuclear localization of the respective proteins in at least 70% of the cells. We used five consecutive transfections to support continuous protein expression resulting in the formation of iPS colonies that express alkaline phosphatase and several ESC markers and that can be expanded. This method completely avoids DNA integration and may be developed to replace the use of DNA vectors in the formation of iPS.

  13. Find Preprints

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Journal preprints may only be available via subscription and thus require Remote Access. Questions? 667-5809 Email Databases arXiv.org - open access eprints in Physics, Mathematics...

  14. Study Finds DOE-Funded Research in Energy Storage Provides a...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Study Finds DOE-Funded Research in Energy Storage Provides a Vital Foundation for Success of Today's Hybrid and Electric Vehicles Study Finds DOE-Funded Research in Energy Storage ...

  15. Alaska Oil and Gas Finding of Best Interest | Open Energy Information

    Open Energy Info (EERE)

    Oil and Gas Finding of Best Interest Jump to: navigation, search OpenEI Reference LibraryAdd to library Web Site: Alaska Oil and Gas Finding of Best Interest Author Alaska...

  16. Scientists at ALS Find New Path to More Efficient Organic Solar...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Scientists at ALS Find New Path to More Efficient Organic Solar Cells Scientists at ALS Find New Path to More Efficient Organic Solar Cells Print Monday, 07 January 2013 00:00 ...

  17. Report on Hydrogen Storage Panel Findings in DOE-BES Sponsored...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Hydrogen Storage Panel Findings in DOE-BES Sponsored Workshop on Basic Research for Hydrogen Production, Storage and Use Report on Hydrogen Storage Panel Findings in DOE-BES...

  18. EA-1800: Notice of Availability of the Finding of No Significant...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Notice of Availability of the Finding of No Significant Impact Monarch Warren County Wind Turbine Project The U.S. Department of Energy (DOE) has issued a Finding of No...

  19. Fact #872: May 11, 2015 Study Finds More than 60% of Millennials...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Car Dealer While Less than Half of Baby Boomers Did - Dataset Fact 872: May 11, 2015 Study Finds More than 60% of Millennials and Generation Xers Use the Internet to Find a Car ...

  20. Fact #872: May 11, 2015 Study Finds More than 60% of Millennials...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    the Internet to Find a Car Dealer While Less than Half of Baby Boomers Did Fact 872: May 11, 2015 Study Finds More than 60% of Millennials and Generation Xers Use the Internet to ...

  1. EI2 Insulation Helps Anxious Pooch Find Calm in the Storm | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    EI2 Insulation Helps Anxious Pooch Find Calm in the Storm EI2 Insulation Helps Anxious Pooch Find Calm in the Storm Photo of a dog sitting on a bed or couch with a stuffed toy in ...

  2. New DOE Report Finds Wind Power Can Serve as Cost-Effective Long...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finds Wind Power Can Serve as Cost-Effective Long-Term Hedge Against Natural Gas Price Increases New DOE Report Finds Wind Power Can Serve as Cost-Effective Long-Term Hedge Against ...

  3. Oestradiol reduces Liver Receptor Homolog-1 mRNA transcript stability in breast cancer cell lines

    SciTech Connect (OSTI)

    Lazarus, Kyren A.; Environmental and Biotechnology Centre, Swinburne University, Hawthorn, Victoria 3122 ; Zhao, Zhe; Knower, Kevin C.; To, Sarah Q.; Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3168 ; Chand, Ashwini L.; Clyne, Colin D.

    2013-08-30

    Highlights: LRH-1 is an orphan nuclear receptor that regulates tumor proliferation. In breast cancer, high mRNA expression is associated with ER+ status. In ER?ve cells, despite very low mRNA, we found abundant LRH-1 protein. Our data show distinctly different LRH-1 protein isoforms in ER? and ER+ breast cancer cells. This is due to differences in LRH-1 mRNA and protein stability rates. -- Abstract: The expression of orphan nuclear receptor Liver Receptor Homolog-1 (LRH-1) is elevated in breast cancer and promotes proliferation, migration and invasion in vitro. LRH-1 expression is regulated by oestrogen (E{sub 2}), with LRH-1 mRNA transcript levels higher in oestrogen receptor ? (ER?) positive (ER+) breast cancer cells compared to ER? cells. However, the presence of LRH-1 protein in ER? cells suggests discordance between mRNA transcript levels and protein expression. To understand this, we investigated the impact of mRNA and protein stability in determining LRH-1 protein levels in breast cancer cells. LRH-1 transcript levels were significantly higher in ER+ versus ER? breast cancer cells lines; however LRH-1 protein was expressed at similar levels. We found LRH-1 mRNA and protein was more stable in ER? compared to ER+ cell lines. The tumor-specific LRH-1 variant isoform, LRH-1v4, which is highly responsive to E{sub 2}, showed increased mRNA stability in ER? versus ER+ cells. In addition, in MCF-7 and T47-D cell lines, LRH-1 total mRNA stability was reduced with E{sub 2} treatment, this effect mediated by ER?. Our data demonstrates that in ER? cells, increased mRNA and protein stability contribute to the abundant protein expression levels. Expression and immunolocalisation of LRH-1 in ER? cells as well as ER? tumors suggests a possible role in the development of ER? tumors. The modulation of LRH-1 bioactivity may therefore be beneficial as a treatment option in both ER? and ER+ breast cancer.

  4. On-chip single-copy real-time reverse-transcription PCR in isolated picoliter droplets

    SciTech Connect (OSTI)

    Beer, N R; Wheeler, E; Lee-Houghton, L; Watkins, N; Nasarabadi, S; Hebert, N; Leung, P; Arnold, D; Bailey, C; Colston, B

    2007-12-19

    The first lab-on-chip system for picoliter droplet generation and RNA isolation, followed by reverse transcription, and PCR amplification with real-time fluorescence detection in the trapped droplets has been developed. The system utilized a shearing T-junction in a fused silica device to generate a stream of monodisperse picoliter-scale droplets that were isolated from the microfluidic channel walls and each other by the oil phase carrier. An off-chip valving system stopped the droplets on-chip, allowing thermal cycling for reverse transcription and subsequent PCR amplification without droplet motion. This combination of the established real-time reverse transcription-PCR assay with digital microfluidics is ideal for isolating single-copy RNA and virions from a complex environment, and will be useful in viral discovery and gene-profiling applications.

  5. Report Summary: Hammer Testing Findings for Solid-State Lighting Luminaires

    SciTech Connect (OSTI)

    2014-01-01

    Brief summary of DOE LED Systems Reliability Consortium's Hammer Testing Findings for Solid-State Lighting Luminaires report.

  6. Summary of Findings: Peer Review of the FY2001 GPRA Assumptions

    Broader source: Energy.gov [DOE]

    Summary of Findings: Peer Review of the FY2001 GPRA Assumptions, Report to National Renewable Energy Laboratory, February 29, 2000.

  7. Summary of Findings: Peer Review of the FY2000 GPRA Assumptions

    Broader source: Energy.gov [DOE]

    Summary of Findings: Peer Review of the FY2000 GPRA Assumptions, Report to National Renewable Energy Laboratory, March 1999.

  8. POSTED BY JOB NAME FIELD OFFICE U.S. DEPARTMENT OF ENERGY ADP TRANSCRIPTION SHEET

    National Nuclear Security Administration (NNSA)

    1 1 1 1 1 1 1 POSTED BY JOB NAME FIELD OFFICE U.S. DEPARTMENT OF ENERGY ADP TRANSCRIPTION SHEET INTERNAL PROJECT TRANSFERS (See instructions for provisions regarding confidentiality.) ACT. CODE PROC. CODE 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 1 SHIPPER RIS TRAN. CODE TO PROJECT NUMBER RECEIVER RIS INTERNAL

  9. POSTED BY JOB NAME FIELD OFFICE U.S. DEPARTMENT OF ENERGY ADP TRANSCRIPTION SHEET

    Energy Savers [EERE]

    1 1 1 1 1 1 1 POSTED BY JOB NAME FIELD OFFICE U.S. DEPARTMENT OF ENERGY ADP TRANSCRIPTION SHEET INTERNAL PROJECT TRANSFERS (See instructions for provisions regarding confidentiality.) ACT. CODE PROC. CODE 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 1 SHIPPER RIS TRAN. CODE TO PROJECT NUMBER RECEIVER RIS INTERNAL

  10. Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus

    DOE Patents [OSTI]

    Dai, Ziyu; Lasure, Linda L; Magnuson, Jon K

    2014-05-27

    The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus.

  11. Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus

    DOE Patents [OSTI]

    Dai, Ziyu; Lasure, Linda L.; Magnuson, Jon K.

    2008-11-11

    The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus.

  12. Isolated Fungal Promoters and Gene Transcription Terminators and Methods of Protein and Chemical Production in a Fungus

    DOE Patents [OSTI]

    Dai, Ziyu; Lasure, Linda L.; Magnuson, Jon K.

    2008-11-11

    The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus.

  13. Ligand-specific transcriptional mechanisms underlie aryl hydrocarbon receptor-mediated developmental toxicity of oxygenated PAHs

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Goodale, B. C.; Geisel School of Medicine at Dartmouth, Hanover, NH; La Du, J.; Tilton, S. C.; Pacific Northwest National Lab.; Sullivan, C. M.; Bisson, W. H.; Waters, K. M.; Tanguay, R. L.

    2015-07-03

    Polycyclic aromatic hydrocarbons (PAHs) are priority environmental contaminants that exhibit mutagenic, carcinogenic, proinflammatory, and teratogenic properties. Oxygen-substituted PAHs (OPAHs) are formed during combustion processes and via phototoxidation and biological degradation of parent (unsubstituted) PAHs. Despite their prevalence both in contaminated industrial sites and in urban air, OPAH mechanisms of action in biological systems are relatively understudied. Like parent PAHs, OPAHs exert structure-dependent mutagenic activities and activation of the aryl hydrocarbon receptor (AHR) and cytochrome p450 metabolic pathway. Four-ring OPAHs 1,9-benz-10-anthrone (BEZO) and benz(a)anthracene-7,12-dione (7,12-B[a]AQ) cause morphological aberrations and induce markers of oxidative stress in developing zebrafish with similar potency, butmore » only 7,12-B[a]AQ induces robust Cyp1a protein expression. We investigated the role of the AHR in mediating the toxicity of BEZO and 7,12-B[a]AQ, and found that knockdown of AHR2 rescued developmental effects caused by both compounds. Using RNA-seq and molecular docking, we identified transcriptional responses that precede developmental toxicity induced via differential interaction with AHR2. Redox-homeostasis genes were affected similarly by these OPAHs, while 7,12-B[a]AQ preferentially activated phase 1 metabolism and BEZO uniquely decreased visual system genes. Analysis of biological functions and upstream regulators suggests that BEZO is a weak AHR agonist, but interacts with other transcriptional regulators to cause developmental toxicity in an AHR-dependent manner. Furthermore, identifying ligand-dependent AHR interactions and signaling pathways is essential for understanding toxicity of this class of environmentally relevant compounds.« less

  14. Ligand-specific transcriptional mechanisms underlie aryl hydrocarbon receptor-mediated developmental toxicity of oxygenated PAHs

    SciTech Connect (OSTI)

    Goodale, B. C.; La Du, J.; Tilton, S. C.; Sullivan, C. M.; Bisson, W. H.; Waters, K. M.; Tanguay, R. L.

    2015-07-03

    Polycyclic aromatic hydrocarbons (PAHs) are priority environmental contaminants that exhibit mutagenic, carcinogenic, proinflammatory, and teratogenic properties. Oxygen-substituted PAHs (OPAHs) are formed during combustion processes and via phototoxidation and biological degradation of parent (unsubstituted) PAHs. Despite their prevalence both in contaminated industrial sites and in urban air, OPAH mechanisms of action in biological systems are relatively understudied. Like parent PAHs, OPAHs exert structure-dependent mutagenic activities and activation of the aryl hydrocarbon receptor (AHR) and cytochrome p450 metabolic pathway. Four-ring OPAHs 1,9-benz-10-anthrone (BEZO) and benz(a)anthracene-7,12-dione (7,12-B[a]AQ) cause morphological aberrations and induce markers of oxidative stress in developing zebrafish with similar potency, but only 7,12-B[a]AQ induces robust Cyp1a protein expression. We investigated the role of the AHR in mediating the toxicity of BEZO and 7,12-B[a]AQ, and found that knockdown of AHR2 rescued developmental effects caused by both compounds. Using RNA-seq and molecular docking, we identified transcriptional responses that precede developmental toxicity induced via differential interaction with AHR2. Redox-homeostasis genes were affected similarly by these OPAHs, while 7,12-B[a]AQ preferentially activated phase 1 metabolism and BEZO uniquely decreased visual system genes. Analysis of biological functions and upstream regulators suggests that BEZO is a weak AHR agonist, but interacts with other transcriptional regulators to cause developmental toxicity in an AHR-dependent manner. Furthermore, identifying ligand-dependent AHR interactions and signaling pathways is essential for understanding toxicity of this class of environmentally relevant compounds.

  15. An integrated approach to reconstructing genome-scale transcriptional regulatory networks

    SciTech Connect (OSTI)

    Imam, Saheed; Noguera, Daniel R.; Donohue, Timothy J.; Leslie, Christina

    2015-02-27

    Transcriptional regulatory networks (TRNs) program cells to dynamically alter their gene expression in response to changing internal or environmental conditions. In this study, we develop a novel workflow for generating large-scale TRN models that integrates comparative genomics data, global gene expression analyses, and intrinsic properties of transcription factors (TFs). An assessment of this workflow using benchmark datasets for the well-studied γ-proteobacterium Escherichia coli showed that it outperforms expression-based inference approaches, having a significantly larger area under the precision-recall curve. Further analysis indicated that this integrated workflow captures different aspects of the E. coli TRN than expression-based approaches, potentially making them highly complementary. We leveraged this new workflow and observations to build a large-scale TRN model for the α-Proteobacterium Rhodobacter sphaeroides that comprises 120 gene clusters, 1211 genes (including 93 TFs), 1858 predicted protein-DNA interactions and 76 DNA binding motifs. We found that ~67% of the predicted gene clusters in this TRN are enriched for functions ranging from photosynthesis or central carbon metabolism to environmental stress responses. We also found that members of many of the predicted gene clusters were consistent with prior knowledge in R. sphaeroides and/or other bacteria. Experimental validation of predictions from this R. sphaeroides TRN model showed that high precision and recall was also obtained for TFs involved in photosynthesis (PpsR), carbon metabolism (RSP_0489) and iron homeostasis (RSP_3341). In addition, this integrative approach enabled generation of TRNs with increased information content relative to R. sphaeroides TRN models built via other approaches. We also show how this approach can be used to simultaneously produce TRN models for each related organism used in the comparative genomics analysis. Our results highlight the advantages of integrating comparative genomics of closely related organisms with gene expression data to assemble large-scale TRN models with high-quality predictions.

  16. An integrated approach to reconstructing genome-scale transcriptional regulatory networks

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Imam, Saheed; Noguera, Daniel R.; Donohue, Timothy J.; Leslie, Christina

    2015-02-27

    Transcriptional regulatory networks (TRNs) program cells to dynamically alter their gene expression in response to changing internal or environmental conditions. In this study, we develop a novel workflow for generating large-scale TRN models that integrates comparative genomics data, global gene expression analyses, and intrinsic properties of transcription factors (TFs). An assessment of this workflow using benchmark datasets for the well-studied γ-proteobacterium Escherichia coli showed that it outperforms expression-based inference approaches, having a significantly larger area under the precision-recall curve. Further analysis indicated that this integrated workflow captures different aspects of the E. coli TRN than expression-based approaches, potentially making themmore » highly complementary. We leveraged this new workflow and observations to build a large-scale TRN model for the α-Proteobacterium Rhodobacter sphaeroides that comprises 120 gene clusters, 1211 genes (including 93 TFs), 1858 predicted protein-DNA interactions and 76 DNA binding motifs. We found that ~67% of the predicted gene clusters in this TRN are enriched for functions ranging from photosynthesis or central carbon metabolism to environmental stress responses. We also found that members of many of the predicted gene clusters were consistent with prior knowledge in R. sphaeroides and/or other bacteria. Experimental validation of predictions from this R. sphaeroides TRN model showed that high precision and recall was also obtained for TFs involved in photosynthesis (PpsR), carbon metabolism (RSP_0489) and iron homeostasis (RSP_3341). In addition, this integrative approach enabled generation of TRNs with increased information content relative to R. sphaeroides TRN models built via other approaches. We also show how this approach can be used to simultaneously produce TRN models for each related organism used in the comparative genomics analysis. Our results highlight the advantages of integrating comparative genomics of closely related organisms with gene expression data to assemble large-scale TRN models with high-quality predictions.« less

  17. Tour of Zero: Helping Customers Find Better Homes at Lower Costs Webinar

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    (Text Version) | Department of Energy Tour of Zero: Helping Customers Find Better Homes at Lower Costs Webinar (Text Version) Tour of Zero: Helping Customers Find Better Homes at Lower Costs Webinar (Text Version) Below is the text version of the webinar Tour of Zero: Helping Customers Find Better Homes at Lower Costs, presented in October 2015. Watch the presentation. Lindsay Parker: Hi, everyone. Welcome to the Department of Energy Zero Energy Ready Home technical training webinar series.

  18. Focus Series: Program Finds Community "Readiness" Is the Key to More

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Retrofits | Department of Energy Program Finds Community "Readiness" Is the Key to More Retrofits Focus Series: Program Finds Community "Readiness" Is the Key to More Retrofits Focus Series: Program Finds Community "Readiness" Is the Key to More Retrofits, a document posted by the U.S. Department of Energy's (DOE'S) Better Buildings Neighborhood Program. PDF icon Focus Series: Michigan More Documents & Publications Better Buildings Network View | June 2014

  19. Fernald Environmental Management Project Director's Final Findings and Orders, October 4, 1995

    Office of Environmental Management (EM)

    Fernald Environmental Management Project Director's Final Findings and Orders, Octobe.. Page 1 of 17 EM Home | Regulatory Compliance | Environmental Compliance Agreements Fernald Environmental Management Project Director's Final Findings and Orders, October 4, 1995 BEFORE THE OHIO ENVIRONMENTAL PROTECTION AGENCY In the Matter Of: United States Department of Energy : Director's Final Fernald Environmental Management Project : Findings and Orders P.O. Box 389705 : Cincinnati, Ohio 45239 :

  20. Portsmouth Gaseous Diffusion Plant Director's Final Findings and Orders, October 4, 1995

    Office of Environmental Management (EM)

    Portsmouth Gaseous Diffusion Plant Director's Final Findings and Orders, October 4, 1995 BEFORE THE OHIO ENVIRONMENTAL PROTECTION AGENCY In the Matter Of: United States Department of Energy : Director's Final Portsmouth Gaseous Diffusion Plant : Findings and Orders P.O. Box 700 : Piketon, Ohio 45661-0700 : Respondent It is hereby agreed by and among the parties hereto as follows: Table of Contents I. Jurisdiction II. Parties Bound III. Definitions IV. Findings of Fact V. Orders VI. Limitations

  1. EA-1950: Finding of No Significant Impact and Mitigation Action Plan |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy 0: Finding of No Significant Impact and Mitigation Action Plan EA-1950: Finding of No Significant Impact and Mitigation Action Plan Grand Coulee-Creston Transmission Line Rebuild; Grant and Lincoln Counties, Washington Bonneville Power Administration issued a finding of no significant impact and a mitigation action plan for rebuilding approximately 28 miles of the Grand Coulee-Creston No. 1 115-kilovolt (kV) transmission line between Coulee Dam in Grant County and

  2. Accident Investigation Board (AIB) findings about the drum breach at WIPP

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Accident Investigation Board findings Accident Investigation Board (AIB) findings about the drum breach at WIPP WHEN: Apr 23, 2015 5:30 PM - 7:00 PM WHERE: Fuller Lodge 2132 Central Avenue, Los Alamos CATEGORY: Community TYPE: Meeting INTERNAL: Calendar Login Event Description The Department of Energy will host a town hall meeting to discuss the Accident Investigation Board findings from the Feb. 14, 2014, drum breach at the Waste Isolation Pilot Plant. Members of the Accident Investigation

  3. EA-1611-S1: Finding of No Significant Impact | Department of...

    Broader source: Energy.gov (indexed) [DOE]

    Project, Logan County, Colorado DOE's Western Area Power Administration issued a Finding of No Significant Impact for the proposed expansion of Colorado Highlands Wind Project,...

  4. How Do You Find Thermal Leaks in Your Home? | Department of Energy

    Energy Savers [EERE]

    Find Thermal Leaks in Your Home? How Do You Find Thermal Leaks in Your Home? March 31, 2011 - 7:30am Addthis On Monday, John told you about the thermal leak detector he purchased to help him find and seal leaks in his home. A thermal leak detector can be a great tool to help you find leaks in your own home, but it's not your only option. In addition to tools like this, you can also use some of our tips on do-it-yourself energy assessments, or you could get a professional energy assessment. How

  5. Six Places to Find Help with Your Energy Costs | Department of...

    Energy Savers [EERE]

    and Efficiency (DSIRE). Here you'll find a comprehensive listing of available rebates, loans, grants, and tax incentives for renewable energy technologies and energy...

  6. EA-1774-S1: Final Environmental Assessment and Finding of No...

    Office of Environmental Management (EM)

    EA-1774-S1: Final Environmental Assessment and Finding of No Significant Impact Energy Conservation Program: Energy Conservation Standards for Direct Heating Equipment (November ...

  7. EA-1815: DOE Notice of Availability of the Finding of No Significant...

    Broader source: Energy.gov (indexed) [DOE]

    environmental impacts associated with the: Cuyahoga County Agricultural Society Wind Energy Project; Berea, Cuyahoga County, Ohio. Notice of Availability of a Finding of No...

  8. How to find fees, taxes, and other charges built into rate in...

    Open Energy Info (EERE)

    and other charges built into rate in different locations Home > Groups > Utility Rate Hi, I am looking to find the rate charges beyond demand, generation, and transmission that...

  9. Clark Fork River Delta Restoration Project 1 Finding of No Significant...

    Broader source: Energy.gov (indexed) [DOE]

    findings for its proposal to provide partial funding to the Idaho Department of Fish and Game's (IDFG) Clark Fork River Delta Restoration Project. The project would involve...

  10. DOE/EA-1976 FINDING OF NO SIGNIFICANT IMPACT FOR PROPOSED CNG...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    DOEEA-1976 FINDING OF NO SIGNIFICANT IMPACT FOR PROPOSED CNG PROJECT REGARDING EMERA CNG, LLC APPLICATION SEEKING DEPARTMENT OF ENERGY AUTHORIZATION TO EXPORT COMPRESSED NATURAL ...

  11. Summary of Findings: Peer Review of the FY2003 GPRA Assumptions

    Broader source: Energy.gov [DOE]

    Summary of Findings: Peer Review of the FY ‘03 GPRA Assumptions, Report to National Renewable Energy Laboratory, Washington, D.C., April 3, 2002.

  12. Idaho IC 61-1701, Legislative Purposes and Findings for the Siting...

    Open Energy Info (EERE)

    701, Legislative Purposes and Findings for the Siting of Certain Electrical Transmission Facilities Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal...

  13. Finding Hidden Oil and Gas Reserves | U.S. DOE Office of Science...

    Office of Science (SC) Website

    Finding Hidden Oil and Gas Reserves Basic Energy Sciences (BES) BES Home About Research ... Michael Commer LBNL MCommer@lbl.gov Funding Basic Research: DOE Office of Science ...

  14. NREL Finds a New Cellulose Digestion Mechanism by a Fast-eating...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    almost twice as fast as the current leading component cellulase enzyme on the market. ... A paper reporting this finding, "Revealing Nature's Cellulase Diversity: The Digestion ...

  15. AS-2, a novel inhibitor of p53-dependent apoptosis, prevents apoptotic mitochondrial dysfunction in a transcription-independent manner and protects mice from a lethal dose of ionizing radiation

    SciTech Connect (OSTI)

    Morita, Akinori; Ariyasu, Shinya; Wang, Bing; Asanuma, Tetsuo; Onoda, Takayoshi; Sawa, Akiko; Tanaka, Kaoru; Takahashi, Ippei; Togami, Shotaro; Nenoi, Mitsuru; Inaba, Toshiya; Aoki, Shin

    2014-08-08

    Highlights: A bidentate HQ derivative, AS-2, suppresses p53-dependent apoptosis by DNA damage. AS-2 does not significantly affect nuclear p53 response. UV-excited blue emission of AS-2 clearly showed its extranuclear localization. AS-2 prevents mitochondrial dysfunction despite the increase of mitochondrial p53. AS-2 protects mice from a radiation dose that causes lethal hematopoietic syndrome. - Abstract: In a previous study, we reported that some tetradentate zinc(II) chelators inhibit p53 through the denaturation of its zinc-requiring structure but a chelator, Bispicen, a potent inhibitor of in vitro apoptosis, failed to show any efficient radioprotective effect against irradiated mice because the toxicity of the chelator to mice. The unsuitability of using tetradentate chelators as radioprotectors prompted us to undertake a more extensive search for p53-inhibiting agents that are weaker zinc(II) chelators and therefore less toxic. Here, we show that an 8-hydroxyquinoline (8HQ) derivative, AS-2, suppresses p53-dependent apoptosis through a transcription-independent mechanism. A mechanistic study using cells with different p53 characteristics revealed that the suppressive effect of AS-2 on apoptosis is specifically mediated through p53. In addition, AS-2 was less effective in preventing p53-mediated transcription-dependent events than pifithrin-? (PFT?), an inhibitor of transcription-independent apoptosis by p53. Fluorescence visualization of the extranuclear distribution of AS-2 also supports that it is ineffective on the transcription-dependent pathway. Further investigations revealed that AS-2 suppressed mitochondrial apoptotic events, such as the mitochondrial release of intermembrane proteins and the loss of mitochondrial membrane potential, although AS-2 resulted in an increase in the mitochondrial translocation of p53 as opposed to the decrease of cytosolic p53, and did not affect the apoptotic interaction of p53 with Bcl-2. AS-2 also protected mice that had been exposed to a lethal dose of ionizing radiation. Our findings indicate that some types of bidentate 8HQ chelators could serve as radioprotectors with no substantial toxicity in vivo.

  16. Global transcriptional, physiological and metabolite analyses of Desulfovibrio vulgaris Hildenborough responses to salt adaptation

    SciTech Connect (OSTI)

    He, Z.; Zhou, A.; Baidoo, E.; He, Q.; Joachimiak, M. P.; Benke, P.; Phan, R.; Mukhopadhyay, A.; Hemme, C.L.; Huang, K.; Alm, E.J.; Fields, M.W.; Wall, J.; Stahl, D.; Hazen, T.C.; Keasling, J.D.; Arkin, A.P.; Zhou, J.

    2009-12-01

    The response of Desulfovibrio vulgaris Hildenborough to salt adaptation (long-term NaCl exposure) was examined by physiological, global transcriptional, and metabolite analyses. The growth of D. vulgaris was inhibited by high levels of NaCl, and the growth inhibition could be relieved by the addition of exogenous amino acids (e.g., glutamate, alanine, tryptophan) or yeast extract. Salt adaptation induced the expression of genes involved in amino acid biosynthesis and transport, electron transfer, hydrogen oxidation, and general stress responses (e.g., heat shock proteins, phage shock proteins, and oxidative stress response proteins). Genes involved in carbon metabolism, cell motility, and phage structures were repressed. Comparison of transcriptomic profiles of D. vulgaris responses to salt adaptation with those of salt shock (short-term NaCl exposure) showed some similarity as well as a significant difference. Metabolite assays showed that glutamate and alanine were accumulated under salt adaptation, suggesting that they may be used as osmoprotectants in D. vulgaris. A conceptual model is proposed to link the observed results to currently available knowledge for further understanding the mechanisms of D. vulgaris adaptation to elevated NaCl.

  17. Automated genomic context analysis and experimental validation platform for discovery of prokaryote transcriptional regulator functions

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Martí-Arbona, Ricardo; Mu, Fangping; Nowak-Lovato, Kristy L.; Wren, Melinda S.; Unkefer, Clifford J.; Unkefer, Pat J.

    2014-12-18

    The clustering of genes in a pathway and the co-location of functionally related genes is widely recognized in prokaryotes. We used these characteristics to predict the metabolic involvement for a Transcriptional Regulator (TR) of unknown function, identified and confirmed its biological activity. A software tool that identifies the genes encoded within a defined genomic neighborhood for the subject TR and its homologs was developed. The output lists of genes in the genetic neighborhoods, their annotated functions, the reactants/products, and identifies the metabolic pathway in which the encoded-proteins function. When a set of TRs of known function was analyzed, we observedmore » that their homologs frequently had conserved genomic neighborhoods that co-located the metabolically related genes regulated by the subject TR. We postulate that TR effectors are metabolites in the identified pathways; indeed the known effectors were present. We analyzed Bxe_B3018 from Burkholderia xenovorans, a TR of unknown function and predicted that this TR was related to the glycine, threonine and serine degradation. We tested the binding of metabolites in these pathways and for those that bound, their ability to modulate TR binding to its specific DNA operator sequence. Using rtPCR, we confirmed that methylglyoxal was an effector of Bxe_3018.« less

  18. Automated genomic context analysis and experimental validation platform for discovery of prokaryote transcriptional regulator functions

    SciTech Connect (OSTI)

    Mart-Arbona, Ricardo; Mu, Fangping; Nowak-Lovato, Kristy L.; Wren, Melinda S.; Unkefer, Clifford J.; Unkefer, Pat J.

    2014-12-18

    The clustering of genes in a pathway and the co-location of functionally related genes is widely recognized in prokaryotes. We used these characteristics to predict the metabolic involvement for a Transcriptional Regulator (TR) of unknown function, identified and confirmed its biological activity. A software tool that identifies the genes encoded within a defined genomic neighborhood for the subject TR and its homologs was developed. The output lists of genes in the genetic neighborhoods, their annotated functions, the reactants/products, and identifies the metabolic pathway in which the encoded-proteins function. When a set of TRs of known function was analyzed, we observed that their homologs frequently had conserved genomic neighborhoods that co-located the metabolically related genes regulated by the subject TR. We postulate that TR effectors are metabolites in the identified pathways; indeed the known effectors were present. We analyzed Bxe_B3018 from Burkholderia xenovorans, a TR of unknown function and predicted that this TR was related to the glycine, threonine and serine degradation. We tested the binding of metabolites in these pathways and for those that bound, their ability to modulate TR binding to its specific DNA operator sequence. Using rtPCR, we confirmed that methylglyoxal was an effector of Bxe_3018.

  19. Energetic Consequences of nitrite stress in Desulfovibrio vulgarisHildenborough, inferred from global transcriptional analysis

    SciTech Connect (OSTI)

    He, Qiang; Huang, Katherine H.; He, Zhili; Alm, Eric J.; Fields,Matthew W.; Hazen, Terry C.; Arkin, Adam P.; Wall, Judy D.; Zhou, Jizhong

    2005-11-03

    Many of the proteins that are candidates for bioenergetic pathways involved with sulfate respiration in Desulfovibrio spp. have been studied, but complete pathways and overall cell physiology remain to be resolved for many environmentally relevant conditions. In order to understand the metabolism of these microorganisms under adverse environmental conditions for improved bioremediation efforts, Desulfovibrio vulgaris Hildenborough was used as a model organism to study stress response to nitrite, an important intermediate in the nitrogen cycle. Previous physiological studies demonstrated that growth was inhibited by nitrite and that nitrite reduction was observed to be the primary mechanism of detoxification. Global transcriptional profiling with whole-genome microarrays revealed coordinated cascades of responses to nitrite in pathways of energy metabolism, nitrogen metabolism, oxidative stress response, and iron homeostasis. In agreement with previous observations, nitrite-stressed cells showed a decrease in the expression of genes encoding sulfate reduction functions in addition to respiratory oxidative phosphorylation and ATP synthase activity. Consequently, the stressed cells had decreased expression of the genes encoding ATP-dependent amino acid transporters and proteins involved in translation. Other genes up-regulated in response to nitrite include the genes in the Fur regulon, which is suggested to be involved in iron homeostasis, and genes in the Per regulon, which is predicted to be responsible for oxidative stress response.

  20. Leptin upregulates telomerase activity and transcription of human telomerase reverse transcriptase in MCF-7 breast cancer cells

    SciTech Connect (OSTI)

    Ren, He; Zhao, Tiansuo; Wang, Xiuchao; Gao, Chuntao; Wang, Jian; Yu, Ming; Hao, Jihui

    2010-03-26

    The aim was to analyze the mechanism of leptin-induced activity of telomerase in MCF-7 breast cancer cells. We found that leptin activated telomerase in a dose-dependent manner; leptin upregulated the expression of Human Telomerase Reverse Transcriptase (hTERT) at mRNA and protein levels; blockade of signal transducer and activator of transcription 3 (STAT3) phosphorylation significantly counteracted leptin-induced hTERT transcription and protein expression; chromatin immunoprecipitation analysis showed that leptin enhanced the binding of STAT3 to the hTERT promoter. This study uncovers a new mechanism of the proliferative effect of leptin on breast cancer cells and provides a new explanation of obesity-related breast cancer.

  1. Find Your STEM Role Model: Join Our Google+ Hangout On March 5 | Department

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of Energy Find Your STEM Role Model: Join Our Google+ Hangout On March 5 Find Your STEM Role Model: Join Our Google+ Hangout On March 5 February 28, 2014 - 12:07pm Addthis Find Your STEM Role Model: Join Our Google+ Hangout On March 5 There's an old axiom that you can't be what you can't see. Perhaps if you think about what inspired you to enter into your current job, or line of study, you'll recognize a role model or two that inspired you to take your current path. That's why NASA and the

  2. V-057: eXtplorer "ext_find_user()" Authentication Bypass Vulnerability |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy 57: eXtplorer "ext_find_user()" Authentication Bypass Vulnerability V-057: eXtplorer "ext_find_user()" Authentication Bypass Vulnerability December 28, 2012 - 6:00am Addthis December 28 2012 - 6:00am PROBLEM: eXtplorer "ext_find_user()" Authentication Bypass Vulnerability PLATFORM: eXtplorer 2.x ABSTRACT: A vulnerability has been reported in eXtplorer, which can be exploited by malicious people to bypass certain security restrictions.

  3. Webinar: Wind-to-Hydrogen Cost Modeling and Project Findings | Department

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of Energy Wind-to-Hydrogen Cost Modeling and Project Findings Webinar: Wind-to-Hydrogen Cost Modeling and Project Findings Below is the text version of the webinar titled "Wind-to-Hydrogen Cost Modeling and Project Findings," originally presented on January 17, 2013. In addition to this text version of the audio, you can access the presentation slides. Moderator: Welcome to today's second attempt at the webinar given by NREL today. So we appreciate you guys that were patient with

  4. DOE ZERH Webinar: Tour of Zero, Helping Customers Find Better Homes at

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Lower Costs | Department of Energy Tour of Zero, Helping Customers Find Better Homes at Lower Costs DOE ZERH Webinar: Tour of Zero, Helping Customers Find Better Homes at Lower Costs Are you interested in helping homebuyers find a better home at a lower cost? The DOE Tour of Zero provides the opportunity for visitors to take a virtual tour of high-performance Zero Energy Ready Homes in every major U.S. climate zone and browse extensive photographs, homeowner testimonials, lists of

  5. EERE 2014 Wind Technologies Market Report Finds Wind Power at Record Low

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Prices | Department of Energy 2014 Wind Technologies Market Report Finds Wind Power at Record Low Prices EERE 2014 Wind Technologies Market Report Finds Wind Power at Record Low Prices August 10, 2015 - 11:00am Addthis EERE 2014 Wind Technologies Market Report Finds Wind Power at Record Low Prices David Friedman Principal Deputy Assistant Secretary for Energy Efficiency and Renewable Energy 2014 proved to be a strong year for the wind industry as the United States remained a global leader in

  6. Find us at the Bay Area Maker Faire | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Find us at the Bay Area Maker Faire Find us at the Bay Area Maker Faire Find us at the Bay Area Maker Faire It's dubbed the "Greatest Show (and Tell) on Earth." Of course we'll be there! From satellites to a 3D-printed Jeep to remote controlled robots to carbon capture microbeads, we'll be displaying some of the coolest technologies from across the Energy Department. We also want to meet innovators like you! Representatives from the Department of Energy's National Labs, the Office of

  7. Experimental Definition and Validation of Protein Coding Transcripts in Chlamydomonas reinhardtii

    SciTech Connect (OSTI)

    Kourosh Salehi-Ashtiani; Jason A. Papin

    2012-01-13

    Algal fuel sources promise unsurpassed yields in a carbon neutral manner that minimizes resource competition between agriculture and fuel crops. Many challenges must be addressed before algal biofuels can be accepted as a component of the fossil fuel replacement strategy. One significant challenge is that the cost of algal fuel production must become competitive with existing fuel alternatives. Algal biofuel production presents the opportunity to fine-tune microbial metabolic machinery for an optimal blend of biomass constituents and desired fuel molecules. Genome-scale model-driven algal metabolic design promises to facilitate both goals by directing the utilization of metabolites in the complex, interconnected metabolic networks to optimize production of the compounds of interest. Using Chlamydomonas reinhardtii as a model, we developed a systems-level methodology bridging metabolic network reconstruction with annotation and experimental verification of enzyme encoding open reading frames. We reconstructed a genome-scale metabolic network for this alga and devised a novel light-modeling approach that enables quantitative growth prediction for a given light source, resolving wavelength and photon flux. We experimentally verified transcripts accounted for in the network and physiologically validated model function through simulation and generation of new experimental growth data, providing high confidence in network contents and predictive applications. The network offers insight into algal metabolism and potential for genetic engineering and efficient light source design, a pioneering resource for studying light-driven metabolism and quantitative systems biology. Our approach to generate a predictive metabolic model integrated with cloned open reading frames, provides a cost-effective platform to generate metabolic engineering resources. While the generated resources are specific to algal systems, the approach that we have developed is not specific to algae and can be readily expanded to other microbial systems as well as higher plants and animals.

  8. Expression of barley SUSIBA2 transcription factor yields high-starch low-methane rice

    SciTech Connect (OSTI)

    Su, J.; Hu, C.; Yan, X.; Jin, Y.; Chen, Z.; Guan, Q.; Wang, Y.; Zhong, D.; Jansson, Georg C.; Wang, F.; Schnrer, Anna; Sun, Chuanxin

    2015-07-22

    Atmospheric methane is the second most important greenhouse gas after carbon dioxide, and is responsible for about 20% of the global warming effect since pre-industrial times. Rice paddies are the largest anthropogenic methane source and produce 7–17% of atmospheric methane. Warm waterlogged soil and exuded nutrients from rice roots provide ideal conditions for methanogenesis in paddies with annual methane emissions of 25–100-million tonnes. This scenario will be exacerbated by an expansion in rice cultivation needed to meet the escalating demand for food in the coming decades4. There is an urgent need to establish sustainable technologies for increasing rice production while reducing methane fluxes from rice paddies. However, ongoing efforts for methane mitigation in rice paddies are mainly based on farming practices and measures that are difficult to implement5. Despite proposed strategies to increase rice productivity and reduce methane emissions4,6, no high-starch low-methane-emission rice has been developed. Here we show that the addition of a single transcription factor gene, barley SUSIBA2, conferred a shift of carbon flux to SUSIBA2 rice, favouring the allocation of photosynthates to aboveground biomass over allocation to roots. The altered allocation resulted in an increased biomass and starch content in the seeds and stems, and suppressed methanogenesis, possibly through a reduction in root exudates. Three-year field trials in China demonstrated that the cultivation of SUSIBA2 rice was associated with a significant reduction in methane emissions and a decrease in rhizospheric methanogen levels. SUSIBA2 rice offers a sustainable means of providing increased starch content for food production while reducing greenhouse gas emissions from rice cultivation. Approaches to increase rice productivity and reduce methane emissions as seen in SUSIBA2 rice may be particularly beneficial in a future climate with rising temperatures resulting in increased methane emissions from paddies.

  9. Comparative phosphoproteomics reveals components of host cell invasion and post-transcriptional regulation during Francisella infection

    SciTech Connect (OSTI)

    Nakayasu, Ernesto S.; Tempel, Rebecca; Cambronne, Xiaolu A.; Petyuk, Vladislav A.; Jones, Marcus B.; Gritsenko, Marina A.; Monroe, Matthew E.; Yang, Feng; Smith, Richard D.; Adkins, Joshua N.; Heffron, Fred

    2013-09-22

    Francisella tularensis is a facultative intracellular bacterium that causes the deadly disease tularemia. Most evidence suggests that Francisella is not well recognized by the innate immune system that normally leads to cytokine expression and cell death. In previous work, we identified new bacterial factors that were hyper-cytotoxic to macrophages. Four of the identified hyper-cytotoxic strains (lpcC, manB, manC and kdtA) had an impaired lipopolysaccharide (LPS) synthesis and produced an exposed lipid A lacking the O-antigen. These mutants were not only hyper-cytotoxic but also were phagocytosed at much higher rates compared to the wild type parent strain. To elucidate the cellular signaling underlying this enhanced phagocytosis and cell death, we performed a large-scale comparative phosphoproteomic analysis of cells infected with wild-type and delta-lpcC F. novicida. Our data suggest that not only actin but also intermediate filaments and microtubules are important for F. novicida entry into the host cells. In addition, we observed differential phosphorylation of tristetraprolin (TTP), a key component of the mRNA-degrading machinery that controls the expression of a variety of genes including many cytokines. Infection with the delta-lpcC mutant induced the hyper-phosphorylation and inhibition of TTP, leading to the production of cytokines such as IL-1beta and TNF-alpha which may kill the host cells by triggering apoptosis. Together, our data provide new insights for Francisella invasion and a post-transcriptional mechanism that prevents the expression of host immune response factors that controls infection by this pathogen.

  10. HPV-18 E2circumflexE4 chimera: 2 new spliced transcripts and proteins induced by keratinocyte differentiation

    SciTech Connect (OSTI)

    Tan, Chye Ling; Gunaratne, Jayantha; Lai, Deborah; Carthagena, Laetitia; Wang, Qian; Xue, Yue Zhen; Quek, Ling Shih; Doorbar, John; Bachelerie, Francoise; Thierry, Francoise; Bellanger, Sophie

    2012-07-20

    The Human Papillomavirus (HPV) E4 is known to be synthesized as an E1circumflexE4 fusion resulting from splice donor and acceptor sites conserved across HPV types. Here we demonstrate the existence of 2 HPV-18 E2circumflexE4 transcripts resulting from 2 splice donor sites in the 5 Prime part of E2, while the splice acceptor site is the one used for E1circumflexE4. Both E2circumflexE4 transcripts are up-regulated by keratinocyte differentiation in vitro and can be detected in clinical samples containing low-grade HPV-18-positive cells from Pap smears. They give rise to two fusion proteins in vitro, E2circumflexE4-S and E2circumflexE4-L. Whereas we could not differentiate E2circumflexE4-S from E1circumflexE4 in vivo, E2circumflexE4-L could be formally identified as a 23 kDa protein in raft cultures in which the corresponding transcript was also found, and in a biopsy from a patient with cervical intraepithelial neoplasia stage I-II (CINI-II) associated with HPV-18, demonstrating the physiological relevance of E2circumflexE4 products.

  11. Finding of No Significant Impact, Federal Register, 73 FR 2017, January 11, 2008

    Broader source: Energy.gov [DOE]

    Document details the finding of no significant impact of the Energy-Efficient Performance Requirements for New Federal Commercial and Residential Buildings, which was published in the Federal Register on January 11, 2008.

  12. Where might I find simplified Data on Capital, O&M, and fuel...

    Open Energy Info (EERE)

    Where might I find simplified Data on Capital, O&M, and fuel costs? Home I teach math to high school seniors. My students are studying energy in all their classes. I have just...

  13. EA-1849-S1: Finding of No Significant Impact | Department of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    EA-1849-S1: Finding of No Significant Impact Phase II Facility - Ormat Tuscarora ... Services for Ormat to construct the Phase II facility at Tuscarora would not have a ...

  14. Nudged-elastic band method with two climbing images: Finding transition states in complex energy landscapes

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Zarkevich, Nikolai A.; Johnson, Duane D.

    2015-01-09

    The nudged-elastic band (NEB) method is modified with concomitant two climbing images (C2-NEB) to find a transition state (TS) in complex energy landscapes, such as those with a serpentine minimal energy path (MEP). If a single climbing image (C1-NEB) successfully finds the TS, then C2-NEB finds it too. Improved stability of C2-NEB makes it suitable for more complex cases, where C1-NEB misses the TS because the MEP and NEB directions near the saddle point are different. Generally, C2-NEB not only finds the TS, but guarantees, by construction, that the climbing images approach it from the opposite sides along the MEP.more » In addition, C2-NEB provides an accuracy estimate from the three images: the highest-energy one and its climbing neighbors. C2-NEB is suitable for fixed-cell NEB and the generalized solid-state NEB.« less

  15. IBEX satellite finds ribbon-like structure at edge of heliosphere

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    IBEX satellite finds ribbon-like structure at edge of heliosphere The NASA IBEX mission ... Using the High Energy Neutral Atom Imager, led by Los Alamos National Laboratory, the NASA ...

  16. Researchers find 3-D printed parts to provide low-cost, custom...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Researchers find 3-D printed parts to provide low-cost, custom alternatives for laboratory equipment By Raphael Rosen February 26, 2015 Tweet Widget Google Plus One Share on...

  17. Sign up to find out what's next for SunShot | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    Sign up to find out how the SunShot Initiative is helping prepare the country for a solar-powered future. Enter your email address here: Subscribe After subscribing, you will ...

  18. In re Kent Pond, No MLP-03-10, Findings of Fact, Conclusions...

    Open Energy Info (EERE)

    re Kent Pond, No MLP-03-10, Findings of Fact, Conclusions of Law, and Order at 8, 13 (Vt. Water Res. Bd. May, 2004) Jump to: navigation, search OpenEI Reference LibraryAdd to...

  19. EA-1861: DOE Notice of Availability of the Finding of No Significant...

    Broader source: Energy.gov (indexed) [DOE]

    Frito Lay Biomass Boiler Project, Beloit, Wisconsin The U.S. Department of Energy (DOE) has issued a Finding of No Significant Impact (FONSI) based on completion of an...

  20. Finding of No Significant Impact for the Storage of Tritium-Producing...

    Broader source: Energy.gov (indexed) [DOE]

    for the Storage of Tritium-Producing Burnable Absorber Rods in K-Area Transfer Bay at the Savannah River Site Agency: U.S. Department of Energy (DOE) Action: Finding of No...

  1. A difficult search, why Basin and Range systems are hard to find...

    Open Energy Info (EERE)

    hard to find Authors M.C. Richards and D.D. Blackwell Published Geothermal Resources Council Bulletin, 2002 DOI Not Provided Check for DOI availability: http:crossref.org...

  2. Tour of Zero: How you can help customers find better homes at...

    Broader source: Energy.gov (indexed) [DOE]

    Description: Are you interested in helping homebuyers find a better home at a lower cost? The DOE Tour of Zero provides the opportunity for visitors to take a virtual tour of...

  3. Tour of Zero: How you can Help Customers Find Better Homes at...

    Broader source: Energy.gov (indexed) [DOE]

    Description: Are you interested in helping homebuyers find a better home at a lower cost? The DOE Tour of Zero provides the opportunity for visitors to take a virtual tour of...

  4. Where can I find APIs and other resources for developers competing...

    Open Energy Info (EERE)

    Where can I find APIs and other resources for developers competing at Cleanweb hackathons? Home > Groups > Developer Submitted by Rmckeel on 25 June, 2012 - 07:23 1 answer Points:...

  5. U.S. Department of Energy Finding of No Significant Impact

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Finding of No Significant Impact Reconstruction of the South Access Road (CR 802) in Support of the Department of Energy, Waste Isolation Pilot Plant in Eddy County, New Mexico AGENCY: U.S. Department of Energy ACTION: Finding of No Significant Impact (FONSI) SUMMARY: The U.S. Department of Energy (DOE), Carlsbad Field Office, has adopted an Environmental Assessment (EA) prepared by the U.S. Department of the Interior Bureau of Land Management (BLM) Carlsbad Field Office for the reconstruction

  6. California Homebuyers Find More Value in Energy-Efficient Labeled Homes |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy California Homebuyers Find More Value in Energy-Efficient Labeled Homes California Homebuyers Find More Value in Energy-Efficient Labeled Homes A photo of a house. During a time when many homeowners have seen their homes' values decrease, some encouraging data shows how investing in efficiency will boost resale value. Aside from the increased comfort and reduced utility bills that stem from home energy efficiency upgrades, homeowners who invest in energy-efficient

  7. Former Construction Worker Finds New Career in Solar | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Former Construction Worker Finds New Career in Solar Former Construction Worker Finds New Career in Solar August 25, 2010 - 4:00pm Addthis Eco Technologies, Inc., hired eleven workers to install these solar panels at the Hillsborough County judicial center. | Photo courtesy of Hillsborough County Eco Technologies, Inc., hired eleven workers to install these solar panels at the Hillsborough County judicial center. | Photo courtesy of Hillsborough County Kevin Craft After Eco Technologies, Inc.,

  8. PART THREE REMEDIAL AND CORRECTIVE ACTIONS ARTICLE XIII. FINDINGS AND DETERMINATIONS

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    29- PART THREE REMEDIAL AND CORRECTIVE ACTIONS ARTICLE XIII. FINDINGS AND DETERMINATIONS 44. The following paragraphs of this Article constitute a summary of the facts upon which EPA and Ecology are proceeding for purposes of Part Three of this Agreement. None of the facts related herein shall be considered admissions by any Party. This Article contains findings by EPA and Ecology, and shall not be used by any person related or unrelated to this Agreement for purposes other than determining the

  9. EMGeo: Risk Minimizing Software for Finding Offshore Fossil Fuels by Fluid

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Identification - Energy Innovation Portal Energy Analysis Energy Analysis Find More Like This Return to Search EMGeo: Risk Minimizing Software for Finding Offshore Fossil Fuels by Fluid Identification Lawrence Berkeley National Laboratory Contact LBL About This Technology Plots of electrical conductivity over the Troll Field in the North Sea produced by analyzing 3D electromagnetic field data. Plots of electrical conductivity over the Troll Field in the North Sea produced by analyzing 3D

  10. DOE/EA-1976 FINDING OF NO SIGNIFICANT IMPACT FOR PROPOSED CNG PROJECT REGARDING

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    DOE/EA-1976 FINDING OF NO SIGNIFICANT IMPACT FOR PROPOSED CNG PROJECT REGARDING EMERA CNG, LLC APPLICATION SEEKING DEPARTMENT OF ENERGY AUTHORIZATION TO EXPORT COMPRESSED NATURAL GAS TO NON-FREE TRADE AGREEMENT NATIONS AGENCY: U.S. Department of Energy, Office of Fossil Energy ACTION: Finding of No Significant Impact SUMMARY: The U.S. Department of Energy (DOE) prepared an environmental assessment (EA) to evaluate the potential environmental impacts associated with the construction and operation

  11. Find Energy Incentives Quicker and Easier with DSIRE Open Data and Website

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    | Department of Energy Incentives Quicker and Easier with DSIRE Open Data and Website Find Energy Incentives Quicker and Easier with DSIRE Open Data and Website March 3, 2015 - 4:16pm Addthis The updated Database of State Incentives for Renewables & Efficiency helps homeowners and businesses find incentive programs that can reduce or defray installation or purchase costs of technologies like photovoltaic systems. | Photo by Dennis Schroeder, National Renewable Energy Laboratory The

  12. ORISE Resources: How to Find Reliable HIV/AIDS Treatment Information on the

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Internet ORISE resource created for NLM assists in finding and evaluating HIV/AIDS information on the Web Produced by the Oak Ridge Institute for Science and Education (ORISE) for the National Library of Medicine, this document identifies websites that provide the most accurate and up-to-date information about HIV/AIDS treatment and research. Consulting quality HIV/AIDS websites is an efficient, inexpensive, and powerful way to find current information. It includes information on clinical

  13. 2012 Congestion Study Webinars to Present Preliminary Findings and Receive Input from Stakeholders

    Broader source: Energy.gov [DOE]

    The Department of Energy will host three webinars in August 2012 to present the preliminary findings of the 2012 National Electric Transmission Congestion Study and to receive input and suggestions from state officials, industry representatives, and other stakeholders. Two of the webinars will be designed to discuss with state officials the initial findings of the DOE 2012 congestion analysis. The third webinar will be for industry representatives and other interested parties, although stakeholders may dial into any of the three meetings.

  14. Fernald Environmental Management Project Director's Final Findings & Orders, June 6, 1996

    Office of Environmental Management (EM)

    . - ~ June 6, 1996 Re: Director's Final Findings & Orders U.S. Department of Energy Cincinnati, Ohio U.S. EPA ID No: OH6890008976 Mr. Phil Hammric U.S. Department of Energy Femald Environmental Management Project P.O. Box 389705 Cincinnati, Ohio 45239 Mr. John Bradbume Femald Environmental Restoration Corporation P.0. Box 538704 Cincinnati, Ohio 45253-8704 Dear Mr. Hammric and Mr. Bradbume: Transmitted herewith are Final Findings and Orders of the Director concrrning the matter indicated

  15. Small Catalyst Finding Could Lead to Big Breakthrough for Fuel Cell

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Deployment | Department of Energy Catalyst Finding Could Lead to Big Breakthrough for Fuel Cell Deployment Small Catalyst Finding Could Lead to Big Breakthrough for Fuel Cell Deployment April 29, 2014 - 3:02pm Addthis Researchers from the Energy Department's Berkeley and Argonne National Labs have developed a new class of fuel cell catalysts that uses roughly 85 percent less platinum and has more than 30 times the catalytic activity than conventional catalysts. The graphic above highlights

  16. Energy Dept. Report Finds Major Potential to Grow Clean, Sustainable U.S.

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Hydropower | Department of Energy Dept. Report Finds Major Potential to Grow Clean, Sustainable U.S. Hydropower Energy Dept. Report Finds Major Potential to Grow Clean, Sustainable U.S. Hydropower April 29, 2014 - 9:15am Addthis News Media Contact (202) 586-4940 WASHINGTON - Building on the Obama Administration's commitment to an all-of-the-above energy strategy, the Energy Department and its Oak Ridge National Laboratory released a renewable energy resource assessment today detailing the

  17. EA-1339: Finding of No Significant Impact (Amended) | Department of Energy

    Office of Environmental Management (EM)

    Finding of No Significant Impact (Amended) EA-1339: Finding of No Significant Impact (Amended) Waste Disposition Activities at Paducah Site, Paducah, Kentucky The U.S. Department of Energy has completed an environmental assessment addendum (DOE/EA-1339-A), which is incorporated herein by reference, for proposed disposition of 17,600 m3 of waste from the Paducah Site in Paducah, Kentucky. It is anticipated that most of the waste would be transported for disposal at various locations in the United

  18. EA-1605: Revised Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    Revised Finding of No Significant Impact EA-1605: Revised Finding of No Significant Impact The Department of Energy prepared an environmental assessment (EA) (DOE/EA-1605) to analyze the potential environmental impacts of the proposed construction and operation of new biomass cogeneration and heating facilities located at the Savannah River Site (SRS). A FONSI was issued in August 2008. In this revised FONSI, DOE proposes to design an construct a new heating plant to be located adjacent to the

  19. G-Zero Finds that Ghostly Strange Quarks Influence Proton Structure |

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Jefferson Lab G-Zero Finds that Ghostly Strange Quarks Influence Proton Structure G-Zero Finds that Ghostly Strange Quarks Influence Proton Structure June 17, 2005 In research performed at the Department of Energy's Jefferson Lab, nuclear physicists have found that strange quarks do contribute to the structure of the proton. This result indicates that, just as previous experiments have hinted, strange quarks in the proton's quark-gluon sea contribute to a proton's properties. The result

  20. Scientists at ALS Find New Path to More Efficient Organic Solar Cells

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Scientists at ALS Find New Path to More Efficient Organic Solar Cells Scientists at ALS Find New Path to More Efficient Organic Solar Cells Print Monday, 07 January 2013 00:00 Harald Ade, a physicist at North Carolina State University, led a study at the Advanced Light Source that revealed a second pathway to improved performances of polymer/organic solar cells. Whereas the first pathway demands crystals of ultrapure domains, the new pathway shows that impure domains if sufficiently small can

  1. DEPARTMENT OF ENERGY FINDING OF NO SIGNIFICANT IMPACT FOR THE MULTIPURPOSE HAUL ROAD

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    DEPARTMENT OF ENERGY FINDING OF NO SIGNIFICANT IMPACT FOR THE MULTIPURPOSE HAUL ROAD WITHIN THE IDAHO NATIONAL LABORATORY SITE (EM-FMDP-I0-068) Agency: U. S. Department of Energy (DOE) Action: Finding of No Significant Impact (FONSI) Summary: The DOE prepared a Draft Environmental Assessment (EA) for the proposed "Environmental Assessment for the Multipurpose Haul Road Within the Idaho National Laboratory Site" (DOE/EA-I772). The proposed action consists of constructing a road for

  2. DOE/EA-1905 FINDING OF NO SIGNIFICANT IMPACT DEPARTMENT OF ENERGY FUNDING FOR

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    905 FINDING OF NO SIGNIFICANT IMPACT DEPARTMENT OF ENERGY FUNDING FOR CITY OF CARLSBAD, NM DOUBLE EAGLE WATER SYSTEM AGENCY: U.S. Department of Energy Carlsbad Field Office (DOE CBFO) ACTION: Finding of No Significant Impact (FONSI) SUMMARY: The DOE CBFO is adopting an Environmental Assessment (EA) prepared by the U.S. Department of Interior Bureau of Land Management (BLM) Carlsbad Field Office for the expansion and upgrade of the City of Carlsbad's Double Eagle Water System. The City of

  3. An evaluation of accounting-based finding costs as efficiency measures for oil and gas exploration

    SciTech Connect (OSTI)

    Boynton, C.E. IV; Boone, J.P.

    1994-08-01

    The authors have operationalized firm-specific exploration efficiency as the difference between a firm-specific intercept estimated in a fixed-effects panel data Cobb-Douglas production frontier model and the maximum firm-specific intercept estimated in that model. The production model was estimated during two different time periods, 1982--1985 and 1989--1992, allowing efficiency to vary intertemporally. This efficiency estimate served as a benchmark against which they compared various measures of inverse finding costs. They assumed that the degree of association with an efficiency benchmark is an important attribute of any finding cost measure and that, further, the degree of association may be used as a metric for choosing between alternative finding cost measures. Accordingly, they evaluated the cross-sectional statistical association between estimated efficiency and alternative inverse finding cost measures. They discovered that the inverse finding cost measure that exhibited the strongest association with efficiency during the two time periods was a three-year moving-average finding cost which included exploration plus development expenditures as costs and reserve extensions and additions plus revisions as the units added.

  4. Estrogen induced concentration dependent differential gene expression in human breast cancer (MCF7) cells: Role of transcription factors

    SciTech Connect (OSTI)

    Chandrasekharan, Sabarinath; Kandasamy, Krishna Kumar; Dayalan, Pavithra; Ramamurthy, Viraragavan

    2013-08-02

    Highlights: Estradiol (E2) at low dose induced cell proliferation in breast cancer cells. E2 at high concentration induced cell stress in breast cancer cells. Estrogen receptor physically interacts only with a few transcription factors. Differential expression of genes with Oct-1 binding sites increased under stress. Transcription factor binding sites showed distinct spatial distribution on genes. -- Abstract: Background: Breast cancer cells respond to estrogen in a concentration dependent fashion, resulting in proliferation or apoptosis. The mechanism of this concentration dependent differential outcome is not well understood yet. Methodology: Meta-analysis of the expression data of MCF7 cells treated with low (1 nM) or high (100 nM) dose of estradiol (E2) was performed. We identified genes differentially expressed at the low or the high dose, and examined the nature of regulatory elements in the vicinity of these genes. Specifically, we looked for the difference in the presence, abundance and spatial distribution of binding sites for estrogen receptor (ER) and selected transcription factors (TFs) in the genomic region up to 25 kb upstream and downstream from the transcription start site (TSS) of these genes. Results: It was observed that at high dose E2 induced the expression of stress responsive genes, while at low dose, genes involved in cell cycle were induced. We found that the occurrence of transcription factor binding regions (TFBRs) for certain factors such as Sp1 and SREBP1 were higher on regulatory regions of genes expressed at low dose. At high concentration of E2, genes with a higher frequency of Oct-1 binding regions were predominantly involved. In addition, there were differences in the spatial distribution pattern of the TFBRs in the genomic regions among the two sets of genes. Discussion: E2 induced predominantly proliferative/metabolic response at low concentrations; but at high concentration, stressrescue responses were induced. At high E2 concentration, classical genomic pathway involving ER binding to the regulatory regions was reduced, and alternate or indirect activation of genes through Oct-1 became more prominent.

  5. AAV-mediated delivery of the transcription factor XBP1s into the striatum reduces mutant Huntingtin aggregation in a mouse model of Huntington's disease

    SciTech Connect (OSTI)

    Zuleta, Amparo [Biomedical Neuroscience Institute, Faculty of Medicine, University of Chile, Santiago (Chile) [Biomedical Neuroscience Institute, Faculty of Medicine, University of Chile, Santiago (Chile); Center for Molecular Studies of the Cell, Institute of Biomedical Sciences, University of Chile, Santiago (Chile); Vidal, Rene L. [Biomedical Neuroscience Institute, Faculty of Medicine, University of Chile, Santiago (Chile) [Biomedical Neuroscience Institute, Faculty of Medicine, University of Chile, Santiago (Chile); Neurounion Biomedical Foundation, Santiago (Chile); Armentano, Donna; Parsons, Geoffrey [Department of Molecular Biology, Genzyme Corporation, 49 New York Avenue, Framingham, MA 01701 (United States)] [Department of Molecular Biology, Genzyme Corporation, 49 New York Avenue, Framingham, MA 01701 (United States); Hetz, Claudio, E-mail: chetz@med.uchile.cl [Biomedical Neuroscience Institute, Faculty of Medicine, University of Chile, Santiago (Chile) [Biomedical Neuroscience Institute, Faculty of Medicine, University of Chile, Santiago (Chile); Center for Molecular Studies of the Cell, Institute of Biomedical Sciences, University of Chile, Santiago (Chile); Department of Immunology and Infectious Diseases, Harvard School of Public Health, 651 Huntington Av., Boston, MA 02446 (United States); Neurounion Biomedical Foundation, Santiago (Chile)

    2012-04-13

    Highlights: Black-Right-Pointing-Pointer The contribution of ER stress to HD has not been directly addressed. Black-Right-Pointing-Pointer Expression of XBP1s using AAVs decreases Huntingtin aggregation in vivo. Black-Right-Pointing-Pointer We describe a new in vivo model of HD based on the expression of a large fragment of mHtt-RFP. -- Abstract: Huntington's disease (HD) is caused by mutations that expand a polyglutamine region in the amino-terminal domain of Huntingtin (Htt), leading to the accumulation of intracellular inclusions and progressive neurodegeneration. Recent reports indicate the engagement of endoplasmic reticulum (ER) stress responses in human HD post mortem samples and animal models of the disease. Adaptation to ER stress is mediated by the activation of the unfolded protein response (UPR), an integrated signal transduction pathway that attenuates protein folding stress by controlling the expression of distinct transcription factors including X-Box binding protein 1 (XBP1). Here we targeted the expression of XBP1 on a novel viral-based model of HD. We delivered an active form of XBP1 locally into the striatum of adult mice using adeno-associated vectors (AAVs) and co-expressed this factor with a large fragment of mutant Htt as a fusion protein with RFP (Htt588{sup Q95}-mRFP) to directly visualize the accumulation of Htt inclusions in the brain. Using this approach, we observed a significant reduction in the accumulation of Htt588{sup Q95}-mRFP intracellular inclusion when XBP1 was co-expressed in the striatum. These results contrast with recent findings indicating a protective effect of XBP1 deficiency in neurodegeneration using knockout mice, and suggest a potential use of gene therapy strategies to manipulate the UPR in the context of HD.

  6. Uncovering Transcriptional Circuits in Arabidoposis by Functional Genomics (2013 DOE JGI Genomics of Energy and Environment 8th Annual User Meeting)

    SciTech Connect (OSTI)

    Pruneda-Paz, Jose [UC San Diego

    2013-03-01

    Jose Pruneda-Paz of UC San Diego on "Uncovering Transcriptional Circuits in Arabidopsis by Functional Genomics" at the 8th Annual Genomics of Energy & Environment Meeting in Walnut Creek, Calif.

  7. Transcript of See Action and Technical Assistance Program Webcast: Energy Audit and Retro-Commissioning Policies for Public and Commercial Buildings

    Broader source: Energy.gov [DOE]

    U.S. Department of Energy Office (DOE) of Energy Efficiency and Renewable Energy (EERE) transcript of See Action and Technical Assistance Program Webcast: Energy Audit and Retro-Commissioning Policies for Public and Commercial Buildings.

  8. Spent nuclear fuel project, Cold Vacuum Drying Facility human factors engineering (HFE) analysis: Results and findings

    SciTech Connect (OSTI)

    Garvin, L.J.

    1998-07-17

    This report presents the background, methodology, and findings of a human factors engineering (HFE) analysis performed in May, 1998, of the Spent Nuclear Fuels (SNF) Project Cold Vacuum Drying Facility (CVDF), to support its Preliminary Safety Analysis Report (PSAR), in responding to the requirements of Department of Energy (DOE) Order 5480.23 (DOE 1992a) and drafted to DOE-STD-3009-94 format. This HFE analysis focused on general environment, physical and computer workstations, and handling devices involved in or directly supporting the technical operations of the facility. This report makes no attempt to interpret or evaluate the safety significance of the HFE analysis findings. The HFE findings presented in this report, along with the results of the CVDF PSAR Chapter 3, Hazards and Accident Analyses, provide the technical basis for preparing the CVDF PSAR Chapter 13, Human Factors Engineering, including interpretation and disposition of findings. The findings presented in this report allow the PSAR Chapter 13 to fully respond to HFE requirements established in DOE Order 5480.23. DOE 5480.23, Nuclear Safety Analysis Reports, Section 8b(3)(n) and Attachment 1, Section-M, require that HFE be analyzed in the PSAR for the adequacy of the current design and planned construction for internal and external communications, operational aids, instrumentation and controls, environmental factors such as heat, light, and noise and that an assessment of human performance under abnormal and emergency conditions be performed (DOE 1992a).

  9. Modeling the effects of vorinostat in vivo reveals both transient and delayed HIV transcriptional activation and minimal killing of latently infected cells

    SciTech Connect (OSTI)

    Ke, Ruian; Lewin, Sharon R.; Elliott, Julian H.; Perelson, Alan S.; Chakraborty, Arup K.

    2015-10-23

    Recent efforts to cure human immunodeficiency virus type-1 (HIV-1) infection have focused on developing latency reversing agents as a first step to eradicate the latent reservoir. The histone deacetylase inhibitor, vorinostat, has been shown to activate HIV RNA transcription in CD4+ T-cells and alter host cell gene transcription in HIV-infected individuals on antiretroviral therapy. In order to understand how latently infected cells respond dynamically to vorinostat treatment and determine the impact of vorinostat on reservoir size in vivo, we have constructed viral dynamic models of latency that incorporate vorinostat treatment. We fitted these models to data collected from a recent clinical trial in which vorinostat was administered daily for 14 days to HIV-infected individuals on suppressive ART. The results show that HIV transcription is increased transiently during the first few hours or days of treatment and that there is a delay before a sustained increase of HIV transcription, whose duration varies among study participants and may depend on the long term impact of vorinostat on host gene expression. Parameter estimation suggests that in latently infected cells, HIV transcription induced by vorinostat occurs at lower levels than in productively infected cells. Lastly, the estimated loss rate of transcriptionally induced cells remains close to baseline in most study participants, suggesting vorinostat treatment does not induce latently infected cell killing and thus reduce the latent reservoir in vivo.

  10. Modeling the effects of vorinostat in vivo reveals both transient and delayed HIV transcriptional activation and minimal killing of latently infected cells

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Ke, Ruian; Lewin, Sharon R.; Elliott, Julian H.; Perelson, Alan S.; Chakraborty, Arup K.

    2015-10-23

    Recent efforts to cure human immunodeficiency virus type-1 (HIV-1) infection have focused on developing latency reversing agents as a first step to eradicate the latent reservoir. The histone deacetylase inhibitor, vorinostat, has been shown to activate HIV RNA transcription in CD4+ T-cells and alter host cell gene transcription in HIV-infected individuals on antiretroviral therapy. In order to understand how latently infected cells respond dynamically to vorinostat treatment and determine the impact of vorinostat on reservoir size in vivo, we have constructed viral dynamic models of latency that incorporate vorinostat treatment. We fitted these models to data collected from a recentmore » clinical trial in which vorinostat was administered daily for 14 days to HIV-infected individuals on suppressive ART. The results show that HIV transcription is increased transiently during the first few hours or days of treatment and that there is a delay before a sustained increase of HIV transcription, whose duration varies among study participants and may depend on the long term impact of vorinostat on host gene expression. Parameter estimation suggests that in latently infected cells, HIV transcription induced by vorinostat occurs at lower levels than in productively infected cells. Lastly, the estimated loss rate of transcriptionally induced cells remains close to baseline in most study participants, suggesting vorinostat treatment does not induce latently infected cell killing and thus reduce the latent reservoir in vivo.« less

  11. Buying a Car? Find Out What it Will REALLY Cost You Each Year | Department

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of Energy a Car? Find Out What it Will REALLY Cost You Each Year Buying a Car? Find Out What it Will REALLY Cost You Each Year November 9, 2009 - 12:52pm Addthis Allison Casey Senior Communicator, NREL If you're in the market for a new car, you've probably been doing a lot of research. Talking to friends, reading reviews, scoping out other cars on the road, maybe even taking some test drives. You probably have a list of things you want in car, and you should consider all of these points of

  12. Finding Energy Efficiency and Savings on a Kentucky Farm | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Finding Energy Efficiency and Savings on a Kentucky Farm Finding Energy Efficiency and Savings on a Kentucky Farm September 28, 2010 - 4:00pm Addthis Maya Payne Smart Former Writer for Energy Empowers, EERE What does this project do? The project is expected to create $852,000 worth of energy savings. Alvin Frogue of Frogue Dairy has been in the dairy business for 50 years and until recently one of his top challenges was managing 250 cows with individualized care. Now $80,540 worth of

  13. EM's West Valley Cleanup Finds Success in History-Making Waste Relocation

    Office of Environmental Management (EM)

    | Department of Energy Finds Success in History-Making Waste Relocation EM's West Valley Cleanup Finds Success in History-Making Waste Relocation November 10, 2015 - 5:00pm Addthis Workers load an overpack container into a vertical storage cask. Workers load an overpack container into a vertical storage cask. WEST VALLEY, N.Y. - For the first time in U.S. history, high-level waste (HLW) was placed in long-term, outdoor storage. The unprecedented accomplishment occurred in EM's cleanup at the

  14. PART TWO PERMITTING/CLOSURE OF TSD UNITS/GROUPS ARTICLE VI. FINDINGS AND DETERMINATIONS

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    16- PART TWO PERMITTING/CLOSURE OF TSD UNITS/GROUPS ARTICLE VI. FINDINGS AND DETERMINATIONS 23. The following paragraphs of this Article constitute a summary of the facts upon which EPA and Ecology are proceeding for purposes of Part Two of this Agreement. None of the facts related herein shall be considered admissions by any Party. This Article contains findings by EPA and Ecology, and shall not be used by any person related or unrelated to this Agreement for purposes other than determining the

  15. DOE Study finds U.S. Wind Industry Competitive, Efficient, and Capable on a

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Global Scale | Department of Energy Study finds U.S. Wind Industry Competitive, Efficient, and Capable on a Global Scale DOE Study finds U.S. Wind Industry Competitive, Efficient, and Capable on a Global Scale September 17, 2014 - 8:37am Addthis Wind turbine blades wind their way by train through Denver. | Photo by Dennis Schroeder of NREL. Wind turbine blades wind their way by train through Denver. | Photo by Dennis Schroeder of NREL. The Global Wind Network Study found that the United

  16. Ars Technica: Finding Smart Ways to Build Smart Things | GE Global Research

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Finding Smart Ways to Build Smart Things Click to email this to a friend (Opens in new window) Share on Facebook (Opens in new window) Click to share (Opens in new window) Click to share on LinkedIn (Opens in new window) Click to share on Tumblr (Opens in new window) Ars Technica: Finding Smart Ways to Build Smart Things Ars Technica visited GE's Global Research Center in Munich to discover the innovative research being done at the facility. Watch a video of Ars Technica's visit below: You Might

  17. Program Finds Unique Way to Fund Energy Upgrades | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Program Finds Unique Way to Fund Energy Upgrades Program Finds Unique Way to Fund Energy Upgrades May 4, 2010 - 11:11am Addthis Stephen Graff Former Writer & editor for Energy Empowers, EERE The $40 million of Recovery Acts funds used for weatherizing more than 6,500 homes over the next two years in Arkansas is a welcomed boost, but Martha Jane Murray of the Clinton Foundation is thinking bigger. "How do we create a more robust delivery system that is not just relying on federal

  18. Find Out How Much Physics You Already Know! at Jefferson Lab's Science

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Series Lecture on Oct. 9 | Jefferson Lab Find Out How Much Physics You Already Know! at Jefferson Lab's Science Series Lecture on Oct. 9 Find Out How Much Physics You Already Know! at Jefferson Lab's Science Series Lecture on Oct. 9 NEWPORT NEWS, VA, Oct. 3, 2012 - The Thomas Jefferson National Accelerator Facility will host two Fall Science Series Lectures this season. The first is set for Tuesday, Oct. 9 and will feature a Jefferson Lab staff member discussing how frequently physics is

  19. Local-global alignment for finding 3D similarities in protein structures

    DOE Patents [OSTI]

    Zemla, Adam T.

    2011-09-20

    A method of finding 3D similarities in protein structures of a first molecule and a second molecule. The method comprises providing preselected information regarding the first molecule and the second molecule. Comparing the first molecule and the second molecule using Longest Continuous Segments (LCS) analysis. Comparing the first molecule and the second molecule using Global Distance Test (GDT) analysis. Comparing the first molecule and the second molecule using Local Global Alignment Scoring function (LGA_S) analysis. Verifying constructed alignment and repeating the steps to find the regions of 3D similarities in protein structures.

  20. Using CEBAF,Physicists Find New Way to Reveal Neutron's Secrets (News @ Old

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Dominion University) | Jefferson Lab Using CEBAF,Physicists Find New Way to Reveal Neutron's Secrets (News @ Old Dominion University) External Link: http://ww2.odu.edu/ao/news/index.php?todo=details&id=32011 By jlab_admin on Thu, 2012-04-26

  1. Find Us Links | OSTI, US Dept of Energy, Office of Scientific and Technical

    Office of Scientific and Technical Information (OSTI)

    Information Find Us Links OSTI Facebook OSTI Twitter OSTI Google+ (Link will open in a new window) Subscribe to the Newsletter Issue issue-3-january-february-2013 Go to full issue-3-january-february-2013 issue Page last updated on 2015-11-24 12:12

  2. Study Finds 54 Gigawatts of Offshore Wind Capacity Technically Possible by 2030

    Broader source: Energy.gov [DOE]

    DOE recently funded a study that finds the deployment of at least 54 gigawatts of offshore wind power to be technically possible by 2030. The National Offshore Wind Energy Grid Interconnection Study (NOWEGIS), which focused on two DOE objectives in reducing barriers to deployment of offshore wind, cost of energy and timeline of deployment.

  3. Fact #872: May 11, 2015 Study Finds More than 60% of Millennials and Generation Xers Use the Internet to Find a Car Dealer While Less than Half of Baby Boomers Did – Dataset

    Broader source: Energy.gov [DOE]

    Excel file and dataset for Study Finds More than 60% of Millennials and Generation Xers Use the Internet to Find a Car Dealer While Less than Half of Baby Boomers Did

  4. NREL Finds Nanotube Semiconductors Well-suited for PV Systems - News

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Releases | NREL NREL Finds Nanotube Semiconductors Well-suited for PV Systems April 25, 2016 Graphic shows cellular nanotubes. Researchers at the Energy Department's National Renewable Energy Laboratory (NREL) discovered single-walled carbon nanotube semiconductors could be favorable for photovoltaic systems because they can potentially convert sunlight to electricity or fuels without losing much energy. The research builds on the Nobel Prize-winning work of Rudolph Marcus, who developed a

  5. Battelle Columbus Laboratories Director's Final Findings and Orders, October 4, 1995 Summary

    Office of Environmental Management (EM)

    Battelle Columbus Laboratories Agreement Name Battelle Columbus Laboratories Director's Final Findings and Orders, October 4, 1995 State Ohio Agreement Type Federal Facility Agreement Legal Driver(s) FFCAct Scope Summary Approve the Compliance Plan Volume of the amended PSTP, hereafter referred to as the "approved STP," and address the generation and treatment of mixed wastes at the Facilities Parties DOE; Ohio Environmental Protection Agency Date 10/4/1995 SCOPE * Approve the

  6. PPPL physicists find clue to formation of magnetic fields around stars and

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    galaxies | Princeton Plasma Physics Lab physicists find clue to formation of magnetic fields around stars and galaxies By Raphael Rosen November 6, 2015 Tweet Widget Google Plus One Share on Facebook An image of coronal loops on the sun that are linked to magnetic fields (Photo by NASA/Solar Dynamics Observatory) An image of coronal loops on the sun that are linked to magnetic fields An enduring astronomical mystery is how stars and galaxies acquire their magnetic fields. Physicists Jonathan

  7. PPPL physicists find clue to formation of magnetic fields around stars and

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    galaxies | Princeton Plasma Physics Lab physicists find clue to formation of magnetic fields around stars and galaxies By Raphael Rosen November 6, 2015 Tweet Widget Google Plus One Share on Facebook An image of coronal loops on the sun that are linked to magnetic fields (Photo by NASA/Solar Dynamics Observatory) An image of coronal loops on the sun that are linked to magnetic fields An enduring astronomical mystery is how stars and galaxies acquire their magnetic fields. Physicists Jonathan

  8. Web Portal Makes Finding Ways to Drive Green Even Easier - News Releases |

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    NREL Web Portal Makes Finding Ways to Drive Green Even Easier From vehicle searches to interactive fuel maps, this Web page delivers October 13, 2009 The U.S. Department of Energy's (DOE) National Renewable Energy Laboratory (NREL) has released a convenient online directory of Web-based tools, database searches, cost calculators, and interactive maps-all related to alternative fuels and advanced vehicles. This comprehensive Web page helps users quickly navigate to the 23 tools, maps, and

  9. ORISE: How to Find Reliable HIV/AIDS Treatment on the Internet

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Find Reliable HIV/AIDS Treatment Information on the Internet August 2011 This document was prepared for the National Library of Medicine by the Oak Ridge Institute for Science and Education through an interagency agreement with the U.S. Department of Energy. ORISE is managed by Oak Ridge Associated Universities under DOE contract number DE-AC06OR23100. Evaluating Health Information on the Internet The World Wide Web contains an abundance of free health information, available 24 hours per day.

  10. ORISE: Wounded veteran finds new way to serve by training for career in 3D

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    printing Wounded veteran finds new way to serve by training for career in 3D printing New training program prepares veterans, others to enter advanced manufacturing workforce FOR IMMEDIATE RELEASE Aug. 4, 2014 FY14-56 Joseph Grabianowski OAK RIDGE, Tenn.-While 26-year-old wounded veteran Joseph Grabianowski has inspired Americans with his harrowing war story, someday he may be nationally known for building highly efficient exhaust systems for cars and trucks using 3D printing technology.

  11. DOE issues Finding of No Significant Impact on Environmental Assessment for

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Replacement Capability for Disposal of Remote-Handled Low Level Radioactive Waste Generated at Idaho Site doe logo U.S. Department of Energy Idaho Operations Office Media Contact: Tim Jackson (208) 526-8484 For Immediate Release December 21, 2011 DOE issues Finding of No Significant Impact on Environmental Assessment for Replacement Capability for Disposal of Remote-Handled Low Level Radioactive Waste Generated at Idaho Site Idaho Falls, ID � After completing a careful assessment, the U.S.

  12. Researchers find 3-D printed parts to provide low-cost, custom alternatives

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    for laboratory equipment | Princeton Plasma Physics Lab Researchers find 3-D printed parts to provide low-cost, custom alternatives for laboratory equipment By Raphael Rosen February 26, 2015 Tweet Widget Google Plus One Share on Facebook 3-D printed parts provide the stands for the aluminum globes in PPPL's Planeterrella, a device that simulates Northern Lights. (Photo by Elle Starkman/PPPL Office of Communications) 3-D printed parts provide the stands for the aluminum globes in PPPL's

  13. Researchers find 3-D printed parts to provide low-cost, custom alternatives

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    for laboratory equipment | Princeton Plasma Physics Lab Researchers find 3-D printed parts to provide low-cost, custom alternatives for laboratory equipment By Raphael Rosen February 26, 2015 Tweet Widget Google Plus One Share on Facebook 3-D printed parts provide the stands for the aluminum globes in PPPL's Planeterrella, a device that simulates Northern Lights. (Photo by Elle Starkman/PPPL Office of Communications) 3-D printed parts provide the stands for the aluminum globes in PPPL's

  14. NREL Tool Finds Effective Behind-the-Meter Energy Storage Configurations -

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    News Releases | NREL Tool Finds Effective Behind-the-Meter Energy Storage Configurations Small battery systems can offer attractive return on investment March 9, 2015 The Energy Department's (DOE) National Renewable Energy Laboratory (NREL) has used the Battery Lifetime Analysis and Simulation Tool (BLAST) to confirm that energy storage for demand-charge management can deliver attractive economic benefits. The analysis paired recent utility rate structures with historic data on solar

  15. Finding the next new tech material: the computational hunt for the weird

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and unusual | The Ames Laboratory Finding the next new tech material: the computational hunt for the weird and unusual Scientists at U.S. Department of Energy's Ames Laboratory are turning to the world of computation to guide their search for the next new material. Their program uses software code developed to map and predict the distinct structural, electronic, magnetic stable and metastable features that are often the source of an advanced material's unique capabilities. "It's the

  16. FINDING OF NO SIGNIFICANT IMPACT FOR SABINE PASS LIQUEFACTION EXPANSION PROJECT REGARDING

    Energy Savers [EERE]

    EXPANSION PROJECT REGARDING SABINE PASS LIQUEFACTION, LLC, APPLICATIONS SEEKING DEPARTMENT OF ENERGY AUTHORIZATION TO EXPORT LIQUEFIED NATURAL GAS FROM SABINE PASS LNG TERMINAL TO NON-FREE TRADE AGREEMENT NATIONS AGENCY: U.S. Department of Energy, Office of Fossil Energy ACTION: Finding of No Significant Impact SUMMARY: Pursuant to section 1501.6 of the regulations of the Council on Environmental Quality (CEQ), 40 CFR 1501.6, the U.S. Department of Energy (DOE) participated as a cooperating

  17. FINDING OF NO SIGNIFICANT IMPACT FOR SABINE PASS LIQUEFACTION PROJECT REGARDING

    Energy Savers [EERE]

    PROJECT REGARDING APPLICATION TO DEPARTMENT OF ENERGY TO INCREASE AUTHORIZED VOLUMES OF LIQUEFIED NATURAL GAS FOR EXPORT FROM SABINE PASS LNG TERMINAL TO NON-FREE TRADE AGREEMENT NATIONS AGENCY: U.S. Department of Energy, Office of Fossil Energy ACTION: Finding of No Significant Impact SUMMARY: Pursuant to section 1508.9 of the regulations of the Council on Environmental Quality (CEQ), 40 CFR 1508.9, the Federal Energy Regulatory Commission (FERC) prepared an environmental assessment (EA) that

  18. *** CANCELED *** SCIENCE ON SATURDAY- "Finding a Needle in A (Genomic)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Haystack or How Can Computers Help Cure Cancer" | Princeton Plasma Physics Lab February 9, 2013, 9:30am Science On Saturday MBG Auditorium *** CANCELED *** SCIENCE ON SATURDAY- "Finding a Needle in A (Genomic) Haystack or How Can Computers Help Cure Cancer" Professor Olga G. Troyanskaya Lewis-Sigler Institute for Integrative Genomics & Department of Computer Science, Princeton University Science on Saturday is a series of lectures given by scientists, mathematicians, and

  19. NNSA scientists find more effective ways to detect nuclear explosions near

    National Nuclear Security Administration (NNSA)

    and far | National Nuclear Security Administration scientists find more effective ways to detect nuclear explosions near and far Wednesday, April 6, 2016 - 10:36am NNSA Blog NNSA activities are vital to detecting nuclear explosions and helping verify compliance with the testing ban worldwide. Recent developments at NNSA's Livermore National Laboratory (LLNL) will help NNSA meet this commitment. Using computer-generated models and field experiments, LLNL simulates how gases produced from

  20. Study finds radioactivity around Los Alamos largely due to natural sources

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Radioactivity largely due to natural sources Study finds radioactivity around Los Alamos largely due to natural sources The study was subsequently peer reviewed externally by scientists at Colorado State University and internally within the Lab. December 10, 2008 Los Alamos National Laboratory sits on top of a once-remote mesa in northern New Mexico with the Jemez mountains as a backdrop to research and innovation covering multi-disciplines from bioscience, sustainable energy sources, to plasma