National Library of Energy BETA

Sample records for find unedited transcripts

  1. QTR - unedited comments | Department of Energy

    Energy Savers [EERE]

    - unedited comments QTR - unedited comments PDF icon Comments received in response to the DOE-QTR Request For Information More Documents & Publications Table of QTR comments in response to Federal Register RFI Table of QTR comments in response to Federal Register RFI QTR table of respondents

  2. Spring 2009 ASA Meeting Disclaimer

    U.S. Energy Information Administration (EIA) Indexed Site

    9 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration In two adjacent files you will find unedited transcripts...

  3. Fall 2005 Meeting of the ASA

    U.S. Energy Information Administration (EIA) Indexed Site

    6 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration In two adjacent files you may find unedited transcripts...

  4. Untitled Document

    U.S. Energy Information Administration (EIA) Indexed Site

    4 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration In an adjacent file you may find an unedited transcript...

  5. Untitled Document

    U.S. Energy Information Administration (EIA) Indexed Site

    5 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration In two adjacent files you may find unedited transcripts...

  6. Finding

    Office of Legacy Management (LM)

    Finding of No Significant Impact Ground Water Compliance at the Grand Junction UMTRA Project Site (Climax Uranium Millsite) September 1999 U.S. Department of Energy Grand Junction Office Grand Junction, Colorado Finding of No Significant Impact Environmental Assessment The U.S. Department of Energy (DOE) proposes a strategy to achieve ground water compliance at the Grand Junction, Colorado, LJMTRA project site, formerly known as the Climax Uranium Millsite. The proposed compliance strategy is no

  7. Spring 2006 ASA Meeting Disclaimer

    Gasoline and Diesel Fuel Update (EIA)

    6 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration In two adjacent files you may find unedited transcripts of EIA's spring 2006 meeting with the American Statistical Association Committee on Energy Statistics. Beginning with the fall 2003 meeting, EIA no longer edits these transcripts. Summaries of previous meetings may be found to the right of the Thursday and Friday transcripts. The public meeting took place April 6 and 7,

  8. Spring 2006 ASA Meeting Disclaimer

    Gasoline and Diesel Fuel Update (EIA)

    7 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration In two adjacent files you will find unedited transcripts of EIA's spring 2007 meeting with the American Statistical Association Committee on Energy Statistics. Beginning with the fall 2003 meeting, EIA no longer edits these transcripts. Summaries of previous meetings can be found to the right of the Thursday and Friday transcripts. The public meeting took place April 19 and

  9. Find Standards

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Standards Find Standards1354608000000Find StandardsIn IHS, please free up a standard for another person: downloadprint the PDF, then Log Out.NoQuestion? 667-5809library@lanl.go...

  10. Find Journals

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and more, use the Find e-Journals search tool. Looking for articles? Try our ArticlesDatabases page. Print Journals Search for print journals in the Library Catalog: Journal in...

  11. Find Patents

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Patents Find Patents Some of these resources are LANL-only and will require Remote Access. Questions? 667-5809 Email US Patents Google Patents - search USPTO patents in a Google interface USPTO Database (United States Patent and Trademark Office) DOE Patents - only patents resulting from DOE-sponsored research Worldwide Patents Espacenet Patent Search (European Patent Office) Free Patents Online SciFinder information (from Chemical Abstracts Service) Ordering Patents Thomson Innovation - contact

  12. Find Clear

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    p align="left"></p> <p align="left"></p> Find Clear Slide Title Duration Status myMail@adobe.com http://Adobe.com More Info>> <p align="left"></p> <p align="left"></p> <p align="left"></p> Go No Matches Found

  13. Fall 2005 Meeting of the ASA

    U.S. Energy Information Administration (EIA) Indexed Site

    5 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration In two adjacent files are the unedited transcripts of...

  14. Find Books

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Books Find Books The Library Catalog is available to the public, though many eBooks are limited to LANL researchers. Question? 667-5809 Email Library Catalog: Search here for all our books, print and electronic Searching the Catalog Search by title, author, subject, ISBN, etc. Locating / Checking out books Screenshot of Library catalog Electronic: Click on the url icon icon. Print: Click on the Location/Call number link (e.g. MAIN: QB614.86 .L37 2006) to see if the book is on the shelf or

  15. EIS-0458: Scoping Meeting Transcript

    Broader source: Energy.gov [DOE]

    Transcript of the public scoping meeting on the draft EIS for the proposed loan guarantee for the First Solar Topaz Solar Farm Project in San Luis Obispo County, CA.

  16. RNA-guided transcriptional regulation

    DOE Patents [OSTI]

    Church, George M.; Mali, Prashant G.; Esvelt, Kevin M.

    2016-02-23

    Methods of modulating expression of a target nucleic acid in a cell are provided including introducing into the cell a first foreign nucleic acid encoding one or more RNAs complementary to DNA, wherein the DNA includes the target nucleic acid, introducing into the cell a second foreign nucleic acid encoding a nuclease-null Cas9 protein that binds to the DNA and is guided by the one or more RNAs, introducing into the cell a third foreign nucleic acid encoding a transcriptional regulator protein or domain, wherein the one or more RNAs, the nuclease-null Cas9 protein, and the transcriptional regulator protein or domain are expressed, wherein the one or more RNAs, the nuclease-null Cas9 protein and the transcriptional regulator protein or domain co-localize to the DNA and wherein the transcriptional regulator protein or domain regulates expression of the target nucleic acid.

  17. Transcript for Building America Video

    Broader source: Energy.gov [DOE]

    Transcript of a video about how DOE’s Building America program is helping to bridge the gap between homes with high energy costs and homes that are healthy, durable, and energy efficient.

  18. Transcription factor-based biosensor

    DOE Patents [OSTI]

    2013-10-08

    The present invention provides for a system comprising a BmoR transcription factor, a .sigma..sup.54-RNA polymerase, and a pBMO promoter operatively linked to a reporter gene, wherein the pBMO promoter is capable of expression of the reporter gene with an activated form of the BmoR and the .sigma..sup.54-RNA polymerase.

  19. Find eBooks

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    eBooks Find eBooks Many of these resources are LANL-only and will require Remote Access. Question? 667-5809 Email The Library Catalog offers the most comprehensive search for scientific Ebooks. The following is a selection of Ebook sources, in case you want to search them directly. [See also Conference Proceedings.] Key eBook Providers Elsevier ScienceDirect - Includes books, book series, and reference works in many scientific disciplines Wiley Online Books, reference works and laboratory

  20. Find LANL Publications

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    LANL Publications Find LANL Publications Some of these resources are LANL-only and will require SSL Portal. Questions? 667-5809 Email Current Lab publications 1663 Actinide Research Quarterly Connections National Security Science Why Lab blogs Internal blogs Los Alamos Report Division/organization publications: ADPSM/WSC Broadcast of Observations Newsletter Engineering Institute Newsletter Lessons Learned and Operating Experience Archive PADSTE STE Highlights Archive VISTAS - highlighting

  1. Financing Residential Energy Efficiency with Carbon Offsets Transcript...

    Energy Savers [EERE]

    Financing Residential Energy Efficiency with Carbon Offsets Transcript Financing Residential Energy Efficiency with Carbon Offsets Transcript This document contains the transcript...

  2. Webinar-Transcript-Repeat.pdf | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Webinar-Transcript-Repeat.pdf Webinar-Transcript-Repeat.pdf PDF icon Webinar-Transcript-Repeat.pdf More Documents & Publications Title Title

  3. Manufacturing Pre-Solicitation Transcript | Department of Energy

    Office of Environmental Management (EM)

    Manufacturing Pre-Solicitation Transcript Manufacturing Pre-Solicitation Transcript Transcript from the US DOE Hydrogen Program Manufacturing R&D Pre-Solicitation Meeting PDF icon pre_solicitation_transcript.pdf More Documents & Publications Transcript from 12/05/08 Transcript from 12/01/08 Public Roundtable- Data Access and Privacy Issues Related to Smart Grid Technologies

  4. Electricity Advisory Committee Meeting, October 29, 2010: Transcript |

    Office of Environmental Management (EM)

    Department of Energy Transcript Electricity Advisory Committee Meeting, October 29, 2010: Transcript Transcript of the Electricity Advisory Committee Meeting held on October 29, 2010. PDF icon Electricity Advisory Committee Meeting, October 29, 2010: Transcript More Documents & Publications Electricity Advisory Committee Meeting, March 10, 2011 - Meeting Minutes and Transcript Electricity Advisory Committee Meeting, July 12, 2011 - Meeting Minutes and Transcript Electricity Advisory

  5. SERC Photovoltaics for Residential Buildings Webinar Transcript...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    PDF icon 20110125pvwebinar.pdf More Documents & Publications Sustainable Energy Resources for Consumers (SERC) - Solar Hot Water Solar Webinar Text Version TAP Webcast Transcript ...

  6. Finding of No Significant Impact

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    U.S. Department of Energy Finding of No Significant Impact 2 June 2001 This page intentionally left blank. U.S. Department of Energy Finding of No Significant Impact 12 June 2001 This page intentionally left blank.

  7. ARM - Research Highlights: Notable Findings

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    HighlightsNotable Research Findings Form Submit a New Research Highlight Sort Highlights Submitter Title Research Area Working Group Submission Date DOE Progress Reports Notable Research Findings for 2001-2006 Office of Science Abstracts Database Research Highlights Summaries Notable Research Findings for the Past Five Years Aerosols Cloud Parameterization and Modeling (Currently Cloud Modeling) Cloud Properties Instantaneous Radiative Flux (Currently Radiative Processes)

  8. csep_transcript_transportation.doc | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    transportation.doc csep_transcript_transportation.doc csep_transcript_transportation.doc Microsoft Office document icon csep_transcript_transportation.doc More Documents & Publications csep_transcript_minneapolis.doc Marketing & Driving Demand: Social Media Tools & Strategies - January 16, 2011 (Text Version) Transcript: Biomass Clean Cities Webinar - Workforce Development

  9. Engaging Financial Institution Partners Transcript.doc | Department of

    Office of Environmental Management (EM)

    Energy Engaging Financial Institution Partners Transcript.doc Engaging Financial Institution Partners Transcript.doc Engaging Financial Institution Partners Transcript.doc Microsoft Office document icon Engaging Financial Institution Partners Transcript.doc More Documents & Publications Engaging Financial Institution Partners Transcript.doc Engaging Financial Institution Partners Case Studies-Financing Energy Improvements on Utility Bills

  10. transcript_jedi_model.doc | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    transcript_jedi_model.doc Microsoft Office document icon transcript_jedi_model.doc More Documents & Publications TAP Webcast Transcript July-29, 2009 Scenario Jedi Tools for Designing & Implementing Better Finance Programs

  11. Electricity Advisory Committee Meeting, September 25, 2008: Transcript |

    Office of Environmental Management (EM)

    Department of Energy Transcript Electricity Advisory Committee Meeting, September 25, 2008: Transcript Transcript of the Electricity Advisory Committee Meeting 3:30 p.m. through 5:55 p.m on Thursday, September 25, 2008. PDF icon Electricity Advisory Committee Meeting, September 25, 2008: Transcript More Documents & Publications Electricity Advisory Committee Meeting, May 20, 2008 (TRANSCRIPT) Electricity Advisory Committee Meeting, October 29, 2010: Transcript Electricity Advisory

  12. TAP Webcast Transcript July-29, 2009

    Broader source: Energy.gov [DOE]

    Transcript of the audio for the July 2009 TAP Webcast titled Economic Impacts of Renewable Energy sponsored by the U.S. Department of Energy (DOE) Technical Assistance Project (TAP) for state and local officials.

  13. Find a Job | Careers | NREL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Find a Job We're looking for scientists, research technicians, engineers, analysts, managers and project leaders, administrators, and communicators, along with information technology, security, facilities, and clerical/administrative support specialists. Interested in joining our team? Find your opportunity We're passionate about our mission-to develop clean energy and energy efficiency technologies and practices, advance related science and engineering, and provide knowledge and innovations to

  14. Find Trainings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Clean Energy Jobs & Career Planning » Find Trainings Find Trainings To pursue a clean energy career, you may need general as well as specialized training. This page has links to training and courses in energy efficiency and renewable energy. This list is a work in progress and is not intended to be all-inclusive or to assure individual program quality. Many of these trainings are local, state or organizational based. Seeking a degree, certificate or education? Check out the Education &

  15. Transcription factor-based biosensors for detecting dicarboxylic acids

    DOE Patents [OSTI]

    Dietrich, Jeffrey; Keasling, Jay

    2014-02-18

    The invention provides methods and compositions for detecting dicarboxylic acids using a transcription factor biosensor.

  16. Residential Retrofit Program Design Guide Overview Transcript.doc |

    Broader source: Energy.gov (indexed) [DOE]

    Department of Energy Residential Retrofit Program Design Guide Overview Transcript.doc Microsoft Office document icon Residential Retrofit Program Design Guide Overview Transcript.doc More Documents & Publications Residential Retrofit Program Design Guide Overview Transcript.doc Residential Retrofit Design Guide Overview Sustainable Energy Resources for Consumers Webinar on Residential Geothermal Heat Pump Retrofit Transcript

  17. csep_transcript_minneapolis.doc | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    minneapolis.doc csep_transcript_minneapolis.doc csep_transcript_minneapolis.doc Microsoft Office document icon csep_transcript_minneapolis.doc More Documents & Publications newguideforcommunityenergystrategicplanning.doc csep_transcript_aspen.doc Marketing & Driving Demand: Social Media Tools & Strategies - January 16, 2011 (Text Version)

  18. Department of Energy (DOE) Webinar Transcript | Department of Energy

    Office of Environmental Management (EM)

    (DOE) Webinar Transcript Department of Energy (DOE) Webinar Transcript PDF icon Department of Energy (DOE) Webinar Transcript More Documents & Publications Welcome to the Department of Energy&#8217;s Recovery Act webinar on reporting in to federalreporting Department of Energy (DOE) Webinar Transcript Registration Overview for OMB Section 1512 Reporting for DOE Prime Recipients

  19. Peak finding using biorthogonal wavelets

    SciTech Connect (OSTI)

    Tan, C.Y.

    2000-02-01

    The authors show in this paper how they can find the peaks in the input data if the underlying signal is a sum of Lorentzians. In order to project the data into a space of Lorentzian like functions, they show explicitly the construction of scaling functions which look like Lorentzians. From this construction, they can calculate the biorthogonal filter coefficients for both the analysis and synthesis functions. They then compare their biorthogonal wavelets to the FBI (Federal Bureau of Investigations) wavelets when used for peak finding in noisy data. They will show that in this instance, their filters perform much better than the FBI wavelets.

  20. Writing audit findings: Be reasonable!

    SciTech Connect (OSTI)

    Girvin, N.W.

    1992-05-01

    A customary approach to auditing and reporting deficiencies is to keep a running list of those that are found, evaluate the severity of each, and based on the evidence, document findings or observations or concerns in an audit report. The report is issued and the auditee is normally requested to address ``root cause`` as part of their corrective action. This paper describes a ``root problems`` approach to documenting audit findings that is designed not only to put the QA auditor in a more favorable light, but to more effectively enable the auditee to identify root cause and meaningful corrective action. The positive results of this approach are considerable. You will have fewer findings but those you do have will be substantial. You will cite requirements that sound reasonable and make arguments difficult. If some of the supporting deficiencies (examples) prove to be incorrect, you will still have ample support for the original finding. You will be seen as reasonable individual who can help lead the auditee towards identification of root cause without taking away part of the responsibility. You even have a fair chance of fostering a sense of commitment to quality improvement on the auditee`s part. This in itself, is its own reward.

  1. Writing audit findings: Be reasonable

    SciTech Connect (OSTI)

    Girvin, N.W.

    1992-05-01

    A customary approach to auditing and reporting deficiencies is to keep a running list of those that are found, evaluate the severity of each, and based on the evidence, document findings or observations or concerns in an audit report. The report is issued and the auditee is normally requested to address root cause'' as part of their corrective action. This paper describes a root problems'' approach to documenting audit findings that is designed not only to put the QA auditor in a more favorable light, but to more effectively enable the auditee to identify root cause and meaningful corrective action. The positive results of this approach are considerable. You will have fewer findings but those you do have will be substantial. You will cite requirements that sound reasonable and make arguments difficult. If some of the supporting deficiencies (examples) prove to be incorrect, you will still have ample support for the original finding. You will be seen as reasonable individual who can help lead the auditee towards identification of root cause without taking away part of the responsibility. You even have a fair chance of fostering a sense of commitment to quality improvement on the auditee's part. This in itself, is its own reward.

  2. Find Internships | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Internships Find Internships Internships in energy efficiency and renewable energy-related fields, such as solar and fuel cells, energy efficiency, atmospheric sciences, ecology, global carbon cycles, climatology, and more, include EERE's own student internship program. Featured Internships Clean Cities University Workforce Development Program Clean Cities logo Science Undergraduate Laboratory Internships (SULI) Fall 2013 Picture of tow people in a labratory working Minority Educational

  3. Transcriptional enhancer from milk protein genes

    DOE Patents [OSTI]

    Casperson, Gerald F.; Schmidhauser, Christian T.; Bissell, Mina J.

    1999-01-01

    The invention relates to novel enhancer nucleotide sequences which stimulate transcription of heterologous DNA in cells in culture. The enhancers are derived from major milk protein genes by the process of deletion mapping and functional analysis. The invention also relates to expression vectors containing the novel enhancers.

  4. FINDING OF NO SIGNIFICANT IMPACT

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    COMMERCIAL DEMONSTRATION OF THE MANUFACTURED AGGREGATE PROCESSING TECHNOLOGY UTILIZING SPRAY DRYER ASH 1 AGENCY: U.S. Department of Energy (DOE) ACTION: Finding of No Significant Impact (FONSI) SUMMARY: The DOE has prepared an Environmental Assessment (EA), DOE/EA-1449, to analyze the potential environmental consequences of participating in a cooperative agreement with Universal Aggregates, LLC, for the design, construction, and operation of a plant to manufacture lightweight aggregates. The

  5. Find Jobs | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Jobs Find Jobs Clean energy jobs can be found in the public, private, and nonprofit sectors and can range from entry-level to professional positions in a wide range of fields. This section can help you familiarize yourself with different types of green energy jobs as you begin your search for a job that meets your interests and skills. EERE Career Opportunities EERE Career Opportunities Energy Efficency and Renewable Energy, the DOE office leading the nation's effort to enhance energy efficiency

  6. Method to determine transcriptional regulation pathways in organisms

    DOE Patents [OSTI]

    Gardner, Timothy S.; Collins, James J.; Hayete, Boris; Faith, Jeremiah

    2012-11-06

    The invention relates to computer-implemented methods and systems for identifying regulatory relationships between expressed regulating polypeptides and targets of the regulatory activities of such regulating polypeptides. More specifically, the invention provides a new method for identifying regulatory dependencies between biochemical species in a cell. In particular embodiments, provided are computer-implemented methods for identifying a regulatory interaction between a transcription factor and a gene target of the transcription factor, or between a transcription factor and a set of gene targets of the transcription factor. Further provided are genome-scale methods for predicting regulatory interactions between a set of transcription factors and a corresponding set of transcriptional target substrates thereof.

  7. HIV transcription is induced in dying cells

    SciTech Connect (OSTI)

    Woloschak, G.E.; Chang-Liu, Chin-Mei; Schreck, S. |; Panozzo, J.; Libertin, C.R.

    1996-02-01

    Using HeLa cells stably transfected with an HIV-LTR-CAT construct, we demonstrated a peak in CAT induction that occurs in viable (but not necessarily cell-division-competent) cells 24 h following exposure to some cell-killing agents. {gamma} rays were the only cell-killing agent which did not induce HIV transcription; this can be attributed to the fact that {gamma}-ray-induced apoptotic death requires functional p53, which is not present in HeLa cells. For all other agents, HIV-LTR induction was dose-dependent and correlated with the amount of cell killing that occurred in the culture. Doses which caused over 99% cell killing induced HIV-LTR transcription maximally, demonstrating that cells that will go on to die by 14 days are the cells expressing HIV-LTR-CAT.

  8. Blackout 2003: Transcript of Technical Conference with the Electric System

    Energy Savers [EERE]

    Investigation Team | Department of Energy Transcript of Technical Conference with the Electric System Investigation Team Blackout 2003: Transcript of Technical Conference with the Electric System Investigation Team Electric System Investigation Team: 8 Reliability Recommendation consultation Transcript of the technical conference sponsored by the U.S. Canada task force investigating the August 14, 2003 blackout across Canada and the Northeastern United States. The transcript includes reports

  9. Financing Residential Energy Efficiency with Carbon Offsets Transcript |

    Energy Savers [EERE]

    Department of Energy Financing Residential Energy Efficiency with Carbon Offsets Transcript Financing Residential Energy Efficiency with Carbon Offsets Transcript This document contains the transcript for the Financing Residential Energy Efficiency with Carbon Offsets webinar held on March 12, 2013. PDF icon financing_residential_energy_efficiency_carbon_offsets_transcript.pdf More Documents & Publications Questions Asked during the Financing Residential Energy Efficiency with Carbon

  10. 20130226_sunshot_transcript_lbnl.doc | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    transcript_lbnl.doc 20130226_sunshot_transcript_lbnl.doc 20130226_sunshot_transcript_lbnl.doc Microsoft Office document icon 20130226_sunshot_transcript_lbnl.doc More Documents & Publications Why Are Resiential PV Prices in Germany So Much Lower Than in the United States? text_alternative_stat_webinar_2012_7_25.docx An Analysis of Residential PV System Price Differences between the United States and Germany

  11. Electricity Advisory Committee Meeting, May 20, 2008 (TRANSCRIPT) |

    Office of Environmental Management (EM)

    Department of Energy (TRANSCRIPT) Electricity Advisory Committee Meeting, May 20, 2008 (TRANSCRIPT) Transcript of the May 20, 2008 Electricity Advisory Committee Meeting including a FERC presentation on the state of electricity markets and a NERC presentation on its 2007 10-year outlook and its 2008 summer assessment. PDF icon Electricity Advisory Committee Meeting, May 20, 2008 (TRANSCRIPT) More Documents & Publications Electricity Advisory Committee Meeting, September 25, 2008:

  12. Department of Energy (DOE) Webinar Transcript

    Broader source: Energy.gov (indexed) [DOE]

    (DOE) Webinar Transcript Recipient Reporting in FederalReporting.gov for Grant, Loan and Cooperative Agreement Recipients Welcome to the Department of Energy's webinar on recipient reporting in FederalReporting.gov for grant, loan and cooperative agreement recipients. Today we're going to cover basic background on the Recovery Act. We'll talk in more detail about OMB reporting requirements in FederalReporting.gov. We'll talk about the timeline, jobs guidance, the actual application

  13. CSC Workshop Transcript 02-20-2105 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Workshop Transcript 02-20-2105 CSC Workshop Transcript 02-20-2105 PDF icon CSC Workshop Transcript 02-20-2105 More Documents & Publications EA-1971: FERC Notice of Intent to Prepare an Environmental Assessment Testimony Office of Information Resources

  14. csep_transcript_aspen.doc | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    aspen.doc csep_transcript_aspen.doc csep_transcript_aspen.doc Microsoft Office document icon csep_transcript_aspen.doc More Documents & Publications highperformanceleasingstrategiesforstateandlocalgovernments.doc Using Social Media to Engage the Community in Energy Efficiency Projects.doc webinar_innovation_permitting_inspections

  15. Regional Transmission Projects: Finding Solutions

    SciTech Connect (OSTI)

    The Keystone Center

    2005-06-15

    The Keystone Center convened and facilitated a year-long Dialogue on "Regional Transmission Projects: Finding Solutions" to develop recommendations that will help address the difficult and contentious issues related to expansions of regional electric transmission systems that are needed for reliable and economic transmission of power within and across regions. This effort brought together a cross-section of affected stakeholders and thought leaders to address the problem with the collective wisdom of their experience and interests. Transmission owners sat at the table with consumer advocates and environmental organizations. Representatives from regional transmission organizations exchanged ideas with state and federal regulators. Generation developers explored common interests with public power suppliers. Together, the Dialogue participants developed consensus solutions about how to begin unraveling some of the more intractable issues surrounding identification of need, allocation of costs, and reaching consensus on siting issues that can frustrate the development of regional transmission infrastructure. The recommendations fall into three broad categories: 1. Recommendations on appropriate institutional arrangements and processes for achieving regional consensus on the need for new or expanded transmission infrastructure 2. Recommendations on the process for siting of transmission lines 3. Recommendations on the tools needed to support regional planning, cost allocation, and siting efforts. List of Dialogue participants: List of Dialogue Participants: American Electric Power American Transmission Company American Wind Energy Association California ISO Calpine Corporation Cinergy Edison Electric Institute Environmental Defense Federal Energy Regulatory Commission Great River Energy International Transmission Company ISO-New England Iowa Public Utility Board Kanner & Associates Midwest ISO National Association of Regulatory Utility Commissioners National Association of State Utility Consumer Advocates National Grid Northeast Utilities PA Office of Consumer Advocates Pacific Gas & Electric Corporation Pennsylvania Public Utility Commission PJM Interconnection The Electricity Consumers Resource Council U.S. Department of Energy US Department of the Interior Van Ness Feldman Western Interstate Energy Board Wind on the Wires Wisconsin Public Service Commission Xcel Energy

  16. Finding Hidden Oil and Gas Reserves

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Finding Hidden Oil and Gas Reserves Finding Hidden Oil and Gas Reserves Key Challenges: Seismic imaging methods, vital in our continuing search for deep offshore oil and gas...

  17. NREL Publishes Gearbox Reliability Collaborative Findings Report...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Publishes Gearbox Reliability Collaborative Findings Report NREL Publishes Gearbox Reliability Collaborative Findings Report October 3, 2011 - 12:56pm Addthis This is an excerpt ...

  18. National Electric Transmission Congestion Study: Preliminary Findings |

    Office of Environmental Management (EM)

    Department of Energy Study: Preliminary Findings National Electric Transmission Congestion Study: Preliminary Findings The Department hosted three webinars in August 2012 to receive input and suggestions concerning the preliminary findings of the National Electric Transmission Congestion Study. The updated presentation used in the webinars is now available. PDF icon National Electric Transmission Congestion Study: Preliminary Findings - updated presentation More Documents & Publications

  19. Public Meeting Transcript: Sustainable Design Standards for Federal

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Buildings | Department of Energy Public Meeting Transcript: Sustainable Design Standards for Federal Buildings Public Meeting Transcript: Sustainable Design Standards for Federal Buildings This transcript is from the public meeting to discuss sustainable design standards for Federal buildings held on July 28, 2010. PDF icon nopr_meeting072810.pdf More Documents & Publications Public Roundtable- Data Access and Privacy Issues Related to Smart Grid Technologies Office of Information

  20. Reducing nontemplated 3' nucleotide addition to polynucleotide transcripts

    DOE Patents [OSTI]

    Kao, C. Cheng (Bloomington, IN)

    2000-01-01

    Non-template 3' nucleotide addition to a transcript is reduced by transcribing a transcript from a template comprising an ultimate and/or penultimate 5' ribose having a C'2 substituent such as methoxy, which reduces non-template 3' nucleotide addition to the transcript. The methods are shown to be applicable to a wide variety of polymerases, including Taq, T7 RNA polymerase, etc.

  1. Unlocking the Mysteries of the J. Robert Oppenheimer Transcript |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Unlocking the Mysteries of the J. Robert Oppenheimer Transcript Unlocking the Mysteries of the J. Robert Oppenheimer Transcript October 3, 2014 - 2:48pm Addthis This is an excerpt from the original press release from the Atomic Energy Commission announcing the publication of the redacted Oppenheimer transcript. | Photo courtesy of the Energy Department. This is an excerpt from the original press release from the Atomic Energy Commission announcing the publication of the

  2. Transcript of March 28, 2013, TAP webinar titled Internal Benchmarking

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Outreach and Data Collection Techniques | Department of Energy Transcript of March 28, 2013, TAP webinar titled Internal Benchmarking Outreach and Data Collection Techniques Transcript of March 28, 2013, TAP webinar titled Internal Benchmarking Outreach and Data Collection Techniques U.S. Department of Energy (DOE) Technical Assistance Program (TAP) transcript of a TAP webinar held on March 28, 2013 and dealing with internal benchmarking outreach and data collection techniques. PDF icon

  3. Blackout 2003: Transcript of Technical Conference with the Electric...

    Broader source: Energy.gov (indexed) [DOE]

    consultation Transcript of the technical conference sponsored by the U.S. Canada task force investigating the August 14, 2003 blackout across Canada and the...

  4. Transcript of March 28, 2013, TAP webinar titled Internal Benchmarking...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    TAP webinar titled Internal Benchmarking Outreach and Data Collection Techniques Transcript of March 28, 2013, TAP webinar titled Internal Benchmarking Outreach and Data ...

  5. Partnering With Utilities to Offer Energy Efficiency Programs Webinar Transcript

    Broader source: Energy.gov [DOE]

    Partnering With Utilities to Offer Energy Efficiency Programs Webinar Transcript, from the U.S. Department of Energy Technical Assistance Program (TAP).

  6. HIV transcription is induced in dying cells

    SciTech Connect (OSTI)

    Woloschak, G.E.; Chang-Liu, Chin-Mei; Schreck, S. |

    1995-06-01

    Using HeLa cells stably transfected with an HIV-LTR-CAT construct, we demonstrated a peak in CAT induction that occurs in viable (but not necessarily cell-division-competent) cells 24 h following exposure to some cell-killing agents. {gamma} rays were the only cell-killing agent which did not induce HIV transcription; this can be attributed to the fact that {gamma}-ray-induced apoptotic death requires functional p53, which is not present in HeLa cells. For all other agents, HIV-LTR induction was dose-dependent and correlated with the amount of cell killing that occurred in the culture. 14 refs., 4 figs., 1 tab.

  7. TE Connectivity Finds Answers in Tomography

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    TE Connectivity Finds Answers in Tomography TE Connectivity Finds Answers in Tomography Print Thursday, 22 August 2013 10:50 TE Connectivity is a world leader in connectivity-the...

  8. Fossil Energy Word Find | Department of Energy

    Office of Environmental Management (EM)

    Word Find Fossil Energy Word Find Word Find (inlcuidng answer key) PDF icon Fossil Energy Word Search More Documents & Publications Fossil Energy Crossword Puzzle Coal Study Guide for Elementary School Guide to Low-Emission Boiler and Combustion Equipment Selection

  9. Transcript of March 4, 2011 Joint Public Meeting | Department of Energy

    Energy Savers [EERE]

    Transcript of March 4, 2011 Joint Public Meeting Transcript of March 4, 2011 Joint Public Meeting Official Transcript of Proceedings - Nuclear Regulatory Commission PDF icon Transcript of March 4, 2011 Joint Public Meeting More Documents & Publications CERTIFICATE OF AUTHENTICITY Office of Information Resources U.S. Offshore Wind Advanced Technology Demonstration Projects Public Meeting Transcript for Offshore Wind Demonstrations

  10. Finding of No Significant Impact (FONSI)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    1 March 2002 FINDING OF NO SIGNIFICANT IMPACT TRANSURANIC WASTE RETRIEVAL FROM THE 218-W-4B AND 218-W-4C LOW-LEVEL BURIAL GROUNDS HANFORD SITE, RICHLAND, WASHINGTON U.S. DEPARTMENT OF ENERGY March 2002 U.S. Department of Energy Finding of No Significant Impact 2 March 2002 This page intentionally left blank. U.S. Department of Energy Finding of No Significant Impact 3 March 2002 AGENCY: U.S. Department of Energy ACTION: Finding of No Significant Impact SUMMARY: The U.S. Department of Energy

  11. Neutrons find "missing" magnetism of plutonium

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Neutrons find "missing" magnetism of plutonium Alumni Link: Opportunities, News and Resources for Former Employees Latest Issue:September 2015 all issues All Issues submit...

  12. ORISE: Forensic scientist finds truth in fingerprints

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Forensic scientist finds truth in fingerprints For more than 30 years, Imogene Van Buren has searched for truth in the friction ridges of fingerprints. She has identified victims...

  13. AAPG Low-Temperature Webinar: Transcript | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AAPG Low-Temperature Webinar: Transcript AAPG Low-Temperature Webinar: Transcript This is a transcript of the AAPG Low-Temperature Webinar held on November 18, 2010. Good day, I am Tim Reinhardt with the Geothermal Technologies Program at the Department of Energy and this is a presentation on Low-Temperature Geothermal Resources. There is a slideshow presentation-PowerPoint that goes along with this briefing and it should be up now and we should be on the cover slide with my name and

  14. Transcript, Keeping the Edge: Enhancing Performance by Managing Culture

    Broader source: Energy.gov [DOE]

    Transcript: 2003 INPO CEO Conference, Keeping the Edge: “Enhancing Performance by Managing Culture” Edgar H. Schein, Ph.D. Professor Emeritis, Massachusetts Institute of Technology November 7, 2003

  15. Method to determine transcriptional regulation pathways in organisms

    Office of Scientific and Technical Information (OSTI)

    (Patent) | SciTech Connect Method to determine transcriptional regulation pathways in organisms Citation Details In-Document Search Title: Method to determine transcriptional regulation pathways in organisms The invention relates to computer-implemented methods and systems for identifying regulatory relationships between expressed regulating polypeptides and targets of the regulatory activities of such regulating polypeptides. More specifically, the invention provides a new method for

  16. Method to determine transcriptional regulation pathways in organisms

    Office of Scientific and Technical Information (OSTI)

    (Patent) | SciTech Connect Method to determine transcriptional regulation pathways in organisms Citation Details In-Document Search Title: Method to determine transcriptional regulation pathways in organisms × You are accessing a document from the Department of Energy's (DOE) SciTech Connect. This site is a product of DOE's Office of Scientific and Technical Information (OSTI) and is provided as a public service. Visit OSTI to utilize additional information resources in energy science and

  17. Common Errors and Innovative Solutions Transcript | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Common Errors and Innovative Solutions Transcript Common Errors and Innovative Solutions Transcript An example of case studies, mainly by showing photos of errors and good examples, then discussing the purpose of the home energy professional guidelines and certification. There may be more examples of what not to do only because these were good learning opportunities. Microsoft Office document icon common_errors_innovative_solutions.doc More Documents & Publications WIPP Weatherization:

  18. Structural basis of JAZ repression of MYC transcription factors in

    Office of Scientific and Technical Information (OSTI)

    jasmonate signalling (Journal Article) | SciTech Connect Structural basis of JAZ repression of MYC transcription factors in jasmonate signalling Citation Details In-Document Search Title: Structural basis of JAZ repression of MYC transcription factors in jasmonate signalling Authors: Zhang, Feng ; Yao, Jian ; Ke, Jiyuan ; Zhang, Li ; Lam, Vinh Q. ; Xin, Xiu-Fang ; Zhou, X. Edward ; Chen, Jian ; Brunzelle, Joseph ; Griffin, Patrick R. ; Zhou, Mingguo ; Xu, H. Eric ; Melcher, Karsten ; He ,

  19. Missourian Finds New Opportunity in Energy Industry

    Broader source: Energy.gov [DOE]

    How one Missouri man decided to take matters into his own hands - creating a company that improves energy efficiency in homes and buildings - after not being able to find work during the recession.

  20. TE Connectivity Finds Answers in Tomography

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    TE Connectivity Finds Answers in Tomography TE Connectivity Finds Answers in Tomography Print Thursday, 22 August 2013 10:50 TE Connectivity is a world leader in connectivity-the $13 billion global company designs and manufactures more than 500,000 different electronic connectivity products for the automotive, energy, industrial, broadband communications, consumer device, healthcare, aerospace, and defense industries. TE Connectivity has a long-standing commitment to innovation and engineering

  1. TE Connectivity Finds Answers in Tomography

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    TE Connectivity Finds Answers in Tomography TE Connectivity Finds Answers in Tomography Print Thursday, 22 August 2013 10:50 TE Connectivity is a world leader in connectivity-the $13 billion global company designs and manufactures more than 500,000 different electronic connectivity products for the automotive, energy, industrial, broadband communications, consumer device, healthcare, aerospace, and defense industries. TE Connectivity has a long-standing commitment to innovation and engineering

  2. Distributed PV Interconnection Recent Analysis Findings

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Recent Analysis Findings Page 1 of 18 Kristen Ardani, Miriam Makhyoun Page 1 of 18 [Speaker: Kristen Ardani] Cover Slide: Good afternoon, everyone. Thank you for joining the Distributed Generation Interconnection Collaborative informational webinar. Today, we are kicking off 2015 with a joint presentation from SEPA and NREL, in which each will discuss recent research and analysis findings related to interconnection. Slide 2: So really, the purpose of today's meeting is to hear recent research

  3. Distributed PV Interconnection: Recent Analysis Findings

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    January 21, 2015 "NREL and SEPA Recent Analysis Findings" Miriam Makhyoun, Solar Electric Power Association (SEPA) Kristen Ardani, National Renewable Energy Laboratory (NREL) 2 Purpose of Today's Meeting * Hear results from SEPA's recent survey of utility interconnection practices. o SEPA finds that utilities confront common challenges as they move towards more streamlined interconnection application processing. * Hear NREL results of forthcoming DGIC data collection and analysis,

  4. Mars Rover finds changing rocks, surprising scientists

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Mars Rover finds changing rocks, surprising scientists Mars Rover finds changing rocks, surprising scientists As NASA's Curiosity rover treks up a three-mile-high mountain on Mars, the rocks are changing. Back on Earth, scientists analyzing the data realized this was something different: It turned out to be the first of the high-silica rocks. December 24, 2015 Mars landscape This color-adjusted composite of images taken by NASA's Curiosity rover in September shows the lower portion of Mount

  5. Transcription Factors Bind Thousands of Active and InactiveRegions in the Drosophila Blastoderm

    SciTech Connect (OSTI)

    Li, Xiao-Yong; MacArthur, Stewart; Bourgon, Richard; Nix, David; Pollard, Daniel A.; Iyer, Venky N.; Hechmer, Aaron; Simirenko, Lisa; Stapleton, Mark; Luengo Hendriks, Cris L.; Chu, Hou Cheng; Ogawa, Nobuo; Inwood, William; Sementchenko, Victor; Beaton, Amy; Weiszmann, Richard; Celniker, Susan E.; Knowles, David W.; Gingeras, Tom; Speed, Terence P.; Eisen, Michael B.; Biggin, Mark D.

    2008-01-10

    Identifying the genomic regions bound by sequence-specific regulatory factors is central both to deciphering the complex DNA cis-regulatory code that controls transcription in metazoans and to determining the range of genes that shape animal morphogenesis. Here, we use whole-genome tiling arrays to map sequences bound in Drosophila melanogaster embryos by the six maternal and gap transcription factors that initiate anterior-posterior patterning. We find that these sequence-specific DNA binding proteins bind with quantitatively different specificities to highly overlapping sets of several thousand genomic regions in blastoderm embryos. Specific high- and moderate-affinity in vitro recognition sequences for each factor are enriched in bound regions. This enrichment, however, is not sufficient to explain the pattern of binding in vivo and varies in a context-dependent manner, demonstrating that higher-order rules must govern targeting of transcription factors. The more highly bound regions include all of the over forty well-characterized enhancers known to respond to these factors as well as several hundred putative new cis-regulatory modules clustered near developmental regulators and other genes with patterned expression at this stage of embryogenesis. The new targets include most of the microRNAs (miRNAs) transcribed in the blastoderm, as well as all major zygotically transcribed dorsal-ventral patterning genes, whose expression we show to be quantitatively modulated by anterior-posterior factors. In addition to these highly bound regions, there are several thousand regions that are reproducibly bound at lower levels. However, these poorly bound regions are, collectively, far more distant from genes transcribed in the blastoderm than highly bound regions; are preferentially found in protein-coding sequences; and are less conserved than highly bound regions. Together these observations suggest that many of these poorly-bound regions are not involved in early-embryonic transcriptional regulation, and a significant proportion may be nonfunctional. Surprisingly, for five of the six factors, their recognition sites are not unambiguously more constrained evolutionarily than the immediate flanking DNA, even in more highly bound and presumably functional regions, indicating that comparative DNA sequence analysis is limited in its ability to identify functional transcription factor targets.

  6. AGENCY: ACTION: REVISED FINDING OF NO SIGNIFICANT IMPACT AND FLOODPLAIN STATEMENT OF FINDINGS

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ACTION: REVISED FINDING OF NO SIGNIFICANT IMPACT AND FLOODPLAIN STATEMENT OF FINDINGS Gila to North Gila Transmission Line Rebuild and Upgrade Project Yuma County, Arizona DOE/EA-1948 U.S. Department of Energy, Western Area Power Administration Revised Finding of No Significant Impact and Floodplain Statement of Findings BACKGROUND: U.S. Department of Energy (DOE), Western Area Power Administration (Western) proposed to rebuild and upgrade the existing 4.8-mile-long Gila to North Gila

  7. Resources for Handling Transcripts | U.S. DOE Office of Science (SC)

    Office of Science (SC) Website

    Graduate Transcripts for Current Graduate Institution » Resources for Handling Transcripts DOE Office of Science Graduate Student Research (SCGSR) Program SCGSR Home Eligibility Benefits Participant Obligations How to Apply Identifying a Collaborating DOE Laboratory Scientist Research Proposal Guidelines Office of Science Priority Research Areas for SCGSR Program Letters of Support Graduate Transcripts for Current Graduate Institution Resources for Handling Transcripts Application Evaluation

  8. Transcript of the Guidelines for Home Energy Professionals project Prezi presentation.

    Broader source: Energy.gov [DOE]

    This document contains the transcript of the Guidelines for Home Energy Professionals project Prezi presentation.

  9. Energy Savings Performance Contracting-Savings Measurement and Verification Transcript 2-24-2011.doc

    Broader source: Energy.gov [DOE]

    Energy Savings Performance Contracting-Savings Measurement and Verification Transcript 2-24-2011.doc

  10. Text Transcript of the Tribal Energy and Economic Development February Webinar: Exploring Your Energy Markets

    Broader source: Energy.gov [DOE]

    Text transcript of the Tribal Energy and Economic Development February Webinar: Exploring Your Energy Markets.

  11. Community Development Finance Institutions-Opportunities for Partnerships with Energy Efficiency Programs Transcript.doc

    Office of Energy Efficiency and Renewable Energy (EERE)

    Community Development Finance Institutions-Opportunities for Partnerships with Energy Efficiency Programs Transcript.doc

  12. Finding a Career in Energy Efficiency

    Broader source: Energy.gov [DOE]

    Kendra went to a county agency hoping they could help her find work, but what she really wanted was a career. That’s when she was referred to an upcoming weatherization training program hosted by the Laborers’ International Union of North America, a half-million strong labor union for construction workers, including weatherization workers.

  13. Occupancy by key transcription factors is a more accurate predictor of enhancer activity than histone modifications or chromatin accessibility

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Dogan, Nergiz; Wu, Weisheng; Morrissey, Christapher S.; Chen, Kuan-Bei; Stonestrom, Aaron; Long, Maria; Keller, Cheryl A.; Cheng, Yong; Jain, Deepti; Visel, Axel; et al

    2015-04-23

    Regulated gene expression controls organismal development, and variation in regulatory patterns has been implicated in complex traits. Thus accurate prediction of enhancers is important for further understanding of these processes. Genome-wide measurement of epigenetic features, such as histone modifications and occupancy by transcription factors, is improving enhancer predictions, but the contribution of these features to prediction accuracy is not known. Given the importance of the hematopoietic transcription factor TAL1 for erythroid gene activation, we predicted candidate enhancers based on genomic occupancy by TAL1 and measured their activity. Contributions of multiple features to enhancer prediction were evaluated based on the resultsmore » of these and other studies. Results: TAL1-bound DNA segments were active enhancers at a high rate both in transient transfections of cultured cells (39 of 79, or 56%) and transgenic mice (43 of 66, or 65%). The level of binding signal for TAL1 or GATA1 did not help distinguish TAL1-bound DNA segments as active versus inactive enhancers, nor did the density of regulation-related histone modifications. A meta-analysis of results from this and other studies (273 tested predicted enhancers) showed that the presence of TAL1, GATA1, EP300, SMAD1, H3K4 methylation, H3K27ac, and CAGE tags at DNase hypersensitive sites gave the most accurate predictors of enhancer activity, with a success rate over 80% and a median threefold increase in activity. Chromatin accessibility assays and the histone modifications H3K4me1 and H3K27ac were sensitive for finding enhancers, but they have high false positive rates unless transcription factor occupancy is also included. Conclusions: Occupancy by key transcription factors such as TAL1, GATA1, SMAD1, and EP300, along with evidence of transcription, improves the accuracy of enhancer predictions based on epigenetic features.« less

  14. finding environmentally friendly materials for MHK devices

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    finding environmentally friendly materials for MHK devices - Sandia Energy Energy Search Icon Sandia Home Locations Contact Us Employee Locator Energy & Climate Secure & Sustainable Energy Future Stationary Power Energy Conversion Efficiency Solar Energy Wind Energy Water Power Supercritical CO2 Geothermal Natural Gas Safety, Security & Resilience of the Energy Infrastructure Energy Storage Nuclear Power & Engineering Grid Modernization Battery Testing Nuclear Fuel Cycle Defense

  15. Jefferson Laboratory Findings Excite Theoreticians, Experimentatlists |

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Jefferson Lab Jefferson Laboratory Findings Excite Theoreticians, Experimentatlists March 1999 Seemingly reasonable assumptions define the human view of the world. But ruled of thumb can mislead - or be altogether incorrect. Fortunately, in physics, most assumptions can be tested. Those that don't measure up are amended. An experiment at the Department of Energy's Thomas Jefferson National Accelerator Facility (Jefferson Lab) has determined that the quark-carried charge distribution on a

  16. Trigger loop folding determines transcription rate of Escherichia colis RNA polymerase

    SciTech Connect (OSTI)

    Mejia, Yara X.; Nudler, Evgeny; Bustamante, Carlos

    2014-12-31

    Two components of the RNA polymerase (RNAP) catalytic center, the bridge helix and the trigger loop (TL), have been linked with changes in elongation rate and pausing. Here, single molecule experiments with the WT and two TL-tip mutants of the Escherichia coli enzyme reveal that tip mutations modulate RNAPs pause-free velocity, identifying TL conformational changes as one of two rate-determining steps in elongation. Consistent with this observation, we find a direct correlation between helix propensity of the modified amino acid and pause-free velocity. Moreover, nucleotide analogs affect transcription rate, suggesting that their binding energy also influences TL folding. A kinetic model in which elongation occurs in two steps, TL folding on nucleoside triphosphate (NTP) binding followed by NTP incorporation/pyrophosphate release, quantitatively accounts for these results. The TL plays no role in pause recovery remaining unfolded during a pause. The model suggests a finely tuned mechanism that balances transcription speed and fidelity.

  17. Trigger loop folding determines transcription rate of Escherichia coli’s RNA polymerase

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Mejia, Yara X.; Nudler, Evgeny; Bustamante, Carlos

    2014-12-31

    Two components of the RNA polymerase (RNAP) catalytic center, the bridge helix and the trigger loop (TL), have been linked with changes in elongation rate and pausing. Here, single molecule experiments with the WT and two TL-tip mutants of the Escherichia coli enzyme reveal that tip mutations modulate RNAP’s pause-free velocity, identifying TL conformational changes as one of two rate-determining steps in elongation. Consistent with this observation, we find a direct correlation between helix propensity of the modified amino acid and pause-free velocity. Moreover, nucleotide analogs affect transcription rate, suggesting that their binding energy also influences TL folding. A kineticmore » model in which elongation occurs in two steps, TL folding on nucleoside triphosphate (NTP) binding followed by NTP incorporation/pyrophosphate release, quantitatively accounts for these results. The TL plays no role in pause recovery remaining unfolded during a pause. The model suggests a finely tuned mechanism that balances transcription speed and fidelity.« less

  18. Best Possible Strategy for Finding Ground States

    SciTech Connect (OSTI)

    Franz, Astrid; Hoffmann, Karl Heinz; Salamon, Peter

    2001-06-04

    Finding the ground state of a system with a complex energy landscape is important for many physical problems including protein folding, spin glasses, chemical clusters, and neural networks. Such problems are usually solved by heuristic search methods whose efficacy is judged by empirical performance on selected examples. We present a proof that, within the large class of algorithms that simulate a random walk on the landscape, threshold accepting is the best possible strategy. In particular, it can perform better than simulated annealing and Tsallis statistics. Our proof is the first example of a provably optimal strategy in this area.

  19. UDAC 2014 Findings and Recommendations | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    2014 Findings and Recommendations UDAC 2014 Findings and Recommendations PDF icon UDAC 2014 Findings and Recommendations More Documents & Publications Minutes from December 16, 2013 UDAC Meeting UDAC 2014 Report UDAC Findings and Recommendations 2014

  20. UDAC Findings and Recommendations 2014 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Findings and Recommendations 2014 UDAC Findings and Recommendations 2014 PDF icon UDAC Findings and Recommendations 2014 More Documents & Publications Minutes from December 16, 2013 UDAC Meeting UDAC 2014 Report UDAC 2014 Findings and Recommendations

  1. Landfill aeration worldwide: Concepts, indications and findings

    SciTech Connect (OSTI)

    Ritzkowski, M.; Stegmann, R.

    2012-07-15

    Highlights: Black-Right-Pointing-Pointer Different landfill aeration concepts and accordant application areas are described. Black-Right-Pointing-Pointer Examples of full scale projects are provided for Europe, North-America and Asia. Black-Right-Pointing-Pointer Major project findings are summarised, including prospects and limitations. Black-Right-Pointing-Pointer Inconsistencies between laboratory and full scale results have been elaborated. Black-Right-Pointing-Pointer An explanatory approach in connection with the inconsistencies is provided. - Abstract: The creation of sustainable landfills is a fundamental goal in waste management worldwide. In this connection landfill aeration contributes towards an accelerated, controlled and sustainable conversion of conventional anaerobic landfills into a biological stabilized state associated with a minimised emission potential. The technology has been successfully applied to landfills in Europe, North America and Asia, following different strategies depending on the geographical region, the specific legislation and the available financial resources. Furthermore, methodologies for the incorporation of landfill aeration into the carbon trade mechanisms have been developed in recent years. This manuscript gives an overview on existing concepts for landfill aeration; their application ranges and specifications. For all of the described concepts examples from different countries worldwide are provided, including details regarding their potentials and limitations. Some of the most important findings from these aeration projects are summarised and future research needs have been identified. It becomes apparent that there is a great demand for a systematisation of the available results and implications in order to further develop and optimise this very promising technology. The IWWG (International Waste Working Group) Task Group 'Landfill Aeration' contributes towards the achievement of this goal.

  2. Structural Basis of Transcription: Role of the Trigger Loop in Substrate

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Specificity and Catalysis of Transcription: Role of the Trigger Loop in Substrate Specificity and Catalysis Transcription is the first step and the key control point in the pathway of gene expression. Transcriptional regulation underlies development, oncogenesis, and other fundamental processes. The central enzyme in transcription is RNA polymerase, in eukaryotic cells there are three forms of RNA polymerase, designated I, II, and III (or A, B, and C), made up of 10-15 polypeptides. The

  3. Meeting of the Electricity Advisory Committee Meeting, September 29-30, 2015- Meeting Minutes and Transcripts

    Broader source: Energy.gov [DOE]

    Meeting minutes and transcripts for the September 29-30, 2015 meeting of the Electric Advisory Committee.

  4. Transcript of Tribal Energy and Economic Webinar: Energy Planning for Tribal Economic Development

    Broader source: Energy.gov [DOE]

    Transcript for the Tribal Energy and Economic Webinar: Energy Planning for Tribal Economic Development held on Jan. 27, 2016.

  5. OSTI News Transcripts, International Group Gathers in Knoxville to Discuss

    Office of Scientific and Technical Information (OSTI)

    Sharing Science across Borders, ETDE, Office of Scientific and Technical Information, U.S. Department of Energy, www.osti.gov | OSTI, US Dept of Energy, Office of Scientific and Technical Information OSTI News Transcripts, International Group Gathers in Knoxville to Discuss Sharing Science across Borders, ETDE, Office of Scientific and Technical Information, U.S. Department of Energy, www.osti.gov International Group Gathers in Knoxville to Discuss Sharing Science across Borders (October

  6. Transcript: OSTI Highlights First-Time Electronic Availability of

    Office of Scientific and Technical Information (OSTI)

    Geothermal Documents at San Diego Geothermal Conference, Office of Scientific and Technical Information, U.S. Department of Energy, www.osti.gov | OSTI, US Dept of Energy, Office of Scientific and Technical Information Transcript: OSTI Highlights First-Time Electronic Availability of Geothermal Documents at San Diego Geothermal Conference, Office of Scientific and Technical Information, U.S. Department of Energy, www.osti.gov September 2006 Geothermal Subject Portal Listen Now Now, more than

  7. Transcription factors for modification of lignin content in plants

    SciTech Connect (OSTI)

    Wang, Huanzhong; Chen, Fang; Dixon, Richard A.

    2015-06-02

    The invention provides methods for modifying lignin, cellulose, xylan, and hemicellulose content in plants, and for achieving ectopic lignification and, for instance, secondary cell wall synthesis in pith cells, by altered regulation of a WRKY transcription factor. Nucleic acid constructs for altered WRKY-TF expression are described. Transgenic plants are provided that comprise modified pith cell walls, and lignin, cellulose, and hemicellulose content. Plants described herein may be used, for example, as improved biofuel feedstock and as highly digestible forage crops.

  8. Graduate Transcripts for Current Graduate Institution | U.S. DOE Office of

    Office of Science (SC) Website

    Science (SC) Graduate Transcripts for Current Graduate Institution DOE Office of Science Graduate Student Research (SCGSR) Program SCGSR Home Eligibility Benefits Participant Obligations How to Apply Identifying a Collaborating DOE Laboratory Scientist Research Proposal Guidelines Office of Science Priority Research Areas for SCGSR Program Letters of Support Graduate Transcripts for Current Graduate Institution Resources for Handling Transcripts Application Evaluation and Selection

  9. Transcriptional control in Alicyclobacillus acidocaldarius and associated genes, proteins, and methods

    DOE Patents [OSTI]

    Lee, Brady Deneys; Thompson, David N; Apel, William A.; Thompson, Vicki Slavchev; Reed, David W; Lacey, Jeffrey A

    2014-05-06

    Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods of modulating transcription or transcription or transcriptional control using isolated and/or purified polypeptides and nucleic acid sequences from Alicyclobacillus acidocaldarius.

  10. Transcriptional control in Alicyclobacillus acidocaldarius and associated genes, proteins, and methods

    DOE Patents [OSTI]

    Lee, Brady D.; Thompson, David N.; Apel, William A.; Thompson, Vicki S.; Reed, David W.; Lacey, Jeffrey A.

    2015-11-17

    Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods of modulating transcription or transcription or transcriptional control using isolated and/or purified polypeptides and nucleic acid sequences from Alicyclobacillus acidocaldarius.

  11. The transcriptional diversity of 25 Drosophila cell lines

    SciTech Connect (OSTI)

    Cherbas, Lucy; Willingham, Aarron; Zhang, Dayu; Yang, Li; Zou, Yi; Eads, Brian D.; Carlson, Joseph W.; Landolin, Jane M.; Kapranov, Philipp; Dumais, Jacqueline; Samsonova, Anastasia; Choi, Jeong-Hyeon; Roberts, Johnny; Davis, Carrie A.; Tang, Haixu; van Baren, Marijke J.; Ghosh, Srinka; Dobin, Alexander; Bell, Kim; Lin, Wei; Langton, Laura; Duff, Michael O.; Tenney, Aaron E.; Zaleski, Chris; Brent, Michael R.; Hoskins, Roger A.; Kaufman, Thomas C.; Andrews, Justen; Graveley, Brenton R.; Perrimon, Norbert; Celniker, Susan E.; Gingeras, Thomas R.; Cherbas, Peter

    2010-11-15

    Drosophila melanogaster cell lines are important resources for cell biologists. Here, we catalog the expression of exons, genes, and unannotated transcriptional signals for 25 lines. Unannotated transcription is substantial (typically 19% of euchromatic signal). Conservatively, we identify 1405 novel transcribed regions; 684 of these appear to be new exons of neighboring, often distant, genes. Sixty-four percent of genes are expressed detectably in at least one line, but only 21% are detected in all lines. Each cell line expresses, on average, 5885 genes, including a common set of 3109. Expression levels vary over several orders of magnitude. Major signaling pathways are well represented: most differentiation pathways are off and survival/growth pathways on. Roughly 50% of the genes expressed by each line are not part of the common set, and these show considerable individuality. Thirty-one percent are expressed at a higher level in at least one cell line than in any single developmental stage, suggesting that each line is enriched for genes characteristic of small sets of cells. Most remarkable is that imaginal discderived lines can generally be assigned, on the basis of expression, to small territories within developing discs. These mappings reveal unexpected stability of even fine-grained spatial determination. No two cell lines show identical transcription factor expression. We conclude that each line has retained features of an individual founder cell superimposed on a common cell line gene expression pattern. Wereport the transcriptional profiles of 25 Drosophila melanogaster cell lines, principally by whole-genome tiling microarray analysis of total RNA, carried out as part of the modENCODE project. The data produced in this study add to our knowledge of the cell lines and of the Drosophila transcriptome in several ways. We summarize the expression of previously annotated genes in each of the 25 lines with emphasis on what those patterns reveal about the origins of the lines and the stability of spatial expression patterns. We also offer an initial analysis of previously unannotated transcripts in the cell lines.

  12. EA-1698: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    8: Finding of No Significant Impact EA-1698: Finding of No Significant Impact Baldwin Wind Energy Center Project Baldwin Wind, LLC has requested to interconnect their proposed new...

  13. EA-1807: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1807: Finding of No Significant Impact Heartland Community College Wind Energy Project, Normal, McLean County, Illinois The Department of...

  14. EA-1819: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1819: Finding of No Significant Impact Kilowatts for Kenston Wind Energy Project, Chagrin Falls, Geauga County, Ohio The Department of Energy's...

  15. EA-1839: Final Environmental Assessment and Finding of No Significant...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    9: Final Environmental Assessment and Finding of No Significant Impact EA-1839: Final Environmental Assessment and Finding of No Significant Impact Department of Energy Loan...

  16. EA-1829: Final Environmental Assessment and Finding of No Significant...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Final Environmental Assessment and Finding of No Significant Impact EA-1829: Final Environmental Assessment and Finding of No Significant Impact Phycal Algae Pilot Project, Wahiawa...

  17. EA-1866: Final Environmental Assessment and Finding of No Significant...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    6: Final Environmental Assessment and Finding of No Significant Impact EA-1866: Final Environmental Assessment and Finding of No Significant Impact Argonne National Laboratory...

  18. EA-1889: Final Environmental Assessment and Finding of No Significant...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Final Environmental Assessment and Finding of No Significant Impact EA-1889: Final Environmental Assessment and Finding of No Significant Impact Disposal of Decommissioned,...

  19. EA-1869: Final Environmental Assessment and Finding of No Significant...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    69: Final Environmental Assessment and Finding of No Significant Impact EA-1869: Final Environmental Assessment and Finding of No Significant Impact General Motors LLC, Electric...

  20. EA-1769: Final Environmental Assessment and Finding of No Significant...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    9: Final Environmental Assessment and Finding of No Significant Impact EA-1769: Final Environmental Assessment and Finding of No Significant Impact Houston Advanced Research...

  1. EA-1784: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    4: Finding of No Significant Impact EA-1784: Finding of No Significant Impact Fotowatio Nevada Solar, LLC's APEX Solar Power Project in Clark County, Nevada The Department of...

  2. EA-1862: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1862: Finding of No Significant Impact Oneida Seven Generations Corporation: Energy Recovery Project, Green Bay, Wisconsin The U.S. Department...

  3. EIS-0372: Record of Decision and Floodplain Statement of Findings...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Record of Decision and Floodplain Statement of Findings EIS-0372: Record of Decision and Floodplain Statement of Findings Bangor Hydro-Electric Company Northeast Reliability...

  4. EIS-0456: Record of Decision and Floodplain Statement of Findings...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Record of Decision and Floodplain Statement of Findings EIS-0456: Record of Decision and Floodplain Statement of Findings Cushman Hydroelectric Project, Mason County, Washington...

  5. Energy Department Report Finds Major Potential to Increase Clean...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Report Finds Major Potential to Increase Clean Hydroelectric Power Energy Department Report Finds Major Potential to Increase Clean Hydroelectric Power April 17, 2012 - 12:39pm ...

  6. EIS-0369: Floodplain Statement of Finding | Department of Energy

    Energy Savers [EERE]

    of Finding EIS-0369: Floodplain Statement of Finding Rail Alignment for the Construction and Operation of a Railroad in Nevada to a Geologic Repository at Yucca Mountain,...

  7. EA-1853: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of No Significant Impact EA-1853: Finding of No Significant Impact Perrin Ranch Wind Energy Interconnection Project, Coconino County, Arizona Based on findings and analysis in...

  8. EA-1923: Mitigated Finding of No Significant Impact | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Mitigated Finding of No Significant Impact EA-1923: Mitigated Finding of No Significant Impact Green Energy School Wind Turbine Project on Saipan, Commonwealth of the Northern ...

  9. EA-1785: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    5: Finding of No Significant Impact EA-1785: Finding of No Significant Impact Phase III Anthropogenic Test Characterization Project, Mobile County, Alabama Based on the analyses in...

  10. EA-1832: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    32: Finding of No Significant Impact EA-1832: Finding of No Significant Impact Rainer Biogas LLC Community Anaerobic Manure Digester, Enumclaw, King County, Washington The U.S....

  11. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact Silver Peak Area Geothermal Exploration Project, Esmeralda County, NV December 7, 2012 EA-1863: Finding of No Significant Impact Vegetation...

  12. EA-1975: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1975: Finding of No Significant Impact LINAC Coherent Light Source-Il, SLAC National Accelerator Laboratory, Menlo Park, California DOE has...

  13. Community Wind Handbook/Find an Installer | Open Energy Information

    Open Energy Info (EERE)

    * Submit Permit Applications * Find an Installer * Purchase Equipment * Plan for Maintenance Find an Installer Homeowners, ranchers, and small businesses can install wind...

  14. EA-1733: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1733: Finding of No Significant Impact Calpine Enhanced Geothermal Systems Project, Sonoma County, California DOE determined that providing...

  15. New Mars rover findings revealed at American Geophysical Union...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Mars rover findings revealed at AGU conference New Mars rover findings revealed at American Geophysical Union conference A group of scientists revealed that the Curiosity rover...

  16. EA-1938: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1938: Finding of No Significant Impact Grieve Unit CO2 Enhanced Recovery Project, Natrona County, WY Western has reviewed BLM's EA "Grieve...

  17. ULTRA-DEEP WATER ADVISORY COMMITTEE FINDINGS AND

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ULTRA-DEEP WATER ADVISORY COMMITTEE FINDINGS AND RECOMMENDATIONS 2014 ULTRA-DEEPWATER ADVISORY COMMITTEE COMMITTEE FINDINGS AND RECOMMENDATIONS i Table of Contents Research and ...

  18. EA-1955: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    EA-1955: Finding of No Significant Impact Campbell County Wind Farm; Campbell County, South Dakota Western Area Power Administration (Western) issued a finding of no significant ...

  19. EA-1802: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    2: Finding of No Significant Impact EA-1802: Finding of No Significant Impact Chicago View Wind Project Chicago Heights, Cook County, Illinois The Department of Energy's Proposed...

  20. EA-1984: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1984: Finding of No Significant Impact Disposition of Five Signature Properties at Idaho National Laboratory DOE has decided to implement the...

  1. Transcript of March 4, 2011 Joint Public Meeting

    Office of Environmental Management (EM)

    Official Transcript of Proceedings NUCLEAR REGULATORY COMMISSION Title: Disposal of Low-Level Nuclear Waste Joint NRC/DOE Workshop Docket Number: (n/a) Location: Phoenix, Arizona Date: Friday, March 4, 2011 Work Order No.: NRC-753 Pages 1-267 NEAL R. GROSS AND CO., INC. Court Reporters and Transcribers 1323 Rhode Island Avenue, N.W. Washington, D.C. 20005 (202) 234-4433 NEAL R. GROSS COURT REPORTERS AND TRANSCRIBERS 1323 RHODE ISLAND AVE., N.W. (202) 234-4433 WASHINGTON, D.C. 20005-3701

  2. Microsoft Word - Chicago Transcript 10 07 08 FINAL.doc

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    /17/2008 Chicago, Illinois Page 1 U.S. Department of Energy Pre-Congestion Study Regional Workshops for the 2009 National Electric Congestion Study Chicago, IL September 17, 2008 8:30 a.m.-12:00 p.m. Transcript David Meyer: Well, good morning, ladies and gentlemen. I'm David Meyer from the Department of Energy. And I welcome you to our workshop on our 2009 congestion study. Before I get started in a brief presentation, I want to introduce some of my colleagues here. Lot Cooke, who's not at this

  3. Untitled Document

    Gasoline and Diesel Fuel Update (EIA)

    3 Meeting of the American Statistical Association Committee on Energy Statistics and the Energy Information Administration This is an unedited Transcript of the Fall Meeting of the Energy Information Administration and the American Statistical Association Committee on Energy Statistics. The meeting took place October 16 and 17, 2003 in the Forrestal Building at 1000 Independence Ave., S.W., Washington, D.C. 20585. Most of the sessions were in 8E-089, although several break out sessions were in

  4. Floodplain Assessment and Statement of Findings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    and Statement of Findings Floodplain Assessment and Statement of Findings Floodplain Assessment and Statement of Findings for Installation of a Renewable Energy Anaerobic Digester Facility at the University of California, Davis in Yolo County, California. PDF icon Statement of Findings More Documents & Publications Floodplain Assessment for Installation of a Renewable Energy Anaerobic Digester Facility

  5. Comparative genomics of transcriptional regulation of methionine metabolism in proteobacteria

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Leyn, Semen A.; Suvorova, Inna A.; Kholina, Tatiana D.; Sherstneva, Sofia S.; Novichkov, Pavel S.; Gelfand, Mikhail S.; Rodionov, Dmitry A.; Kuipers, Oscar P.

    2014-11-20

    Methionine metabolism and uptake genes in Proteobacteria are controlled by a variety of RNA and DNA regulatory systems. We have applied comparative genomics to reconstruct regulons for three known transcription factors, MetJ, MetR, and SahR, and three known riboswitch motifs, SAH, SAM-SAH, and SAM_alpha, in ~200 genomes from 22 taxonomic groups of Proteobacteria. We also identified two novel regulons: a SahR-like transcription factor SamR controlling various methionine biosynthesis genes in the Xanthomonadales group, and a potential RNA regulatory element with terminator-antiterminator mechanism controlling the metX or metZ genes in beta-proteobacteria. For each analyzed regulator we identified the core, taxon-specific andmore » genome-specific regulon members. By analyzing the distribution of these regulators in bacterial genomes and by comparing their regulon contents we elucidated possible evolutionary scenarios for the regulation of the methionine metabolism genes in Proteobacteria.« less

  6. Comparative genomics of transcriptional regulation of methionine metabolism in proteobacteria

    SciTech Connect (OSTI)

    Leyn, Semen A.; Suvorova, Inna A.; Kholina, Tatiana D.; Sherstneva, Sofia S.; Novichkov, Pavel S.; Gelfand, Mikhail S.; Rodionov, Dmitry A.; Kuipers, Oscar P.

    2014-11-20

    Methionine metabolism and uptake genes in Proteobacteria are controlled by a variety of RNA and DNA regulatory systems. We have applied comparative genomics to reconstruct regulons for three known transcription factors, MetJ, MetR, and SahR, and three known riboswitch motifs, SAH, SAM-SAH, and SAM_alpha, in ~200 genomes from 22 taxonomic groups of Proteobacteria. We also identified two novel regulons: a SahR-like transcription factor SamR controlling various methionine biosynthesis genes in the Xanthomonadales group, and a potential RNA regulatory element with terminator-antiterminator mechanism controlling the metX or metZ genes in beta-proteobacteria. For each analyzed regulator we identified the core, taxon-specific and genome-specific regulon members. By analyzing the distribution of these regulators in bacterial genomes and by comparing their regulon contents we elucidated possible evolutionary scenarios for the regulation of the methionine metabolism genes in Proteobacteria.

  7. A new chapter in the transcription SAGA (Journal Article) | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    A new chapter in the transcription SAGA Citation Details In-Document Search Title: A new chapter in the transcription SAGA Eukaryotic transcriptional coactivators are multi-subunit complexes that both modify chromatin and recognize histone modifications. Until recently, structural information on these large complexes has been limited to isolated enzymatic domains or chromatin-binding motifs. This review summarizes recent structural studies of the SAGA coactivator complex that have greatly

  8. June 2014 Electricity Advisory Committee Meeting Summaries and Transcripts Now Available

    Broader source: Energy.gov [DOE]

    The meeting summaries and transcripts for the Electricity Advisory Committee (EAC) meeting held on June 16-17, 2014, are now available.

  9. Successful Partnerships and Lessons from the Field, Part 1, Webinar Transcript

    Broader source: Energy.gov [DOE]

    Part 1-Successful Partnerships and Lessons from the Field Webinar Transcript, from the U.S. Department of Energy Technical Assistance Program (TAP).

  10. Transcript of Office of Indian Energy Webinar: Inter-Tribal Technical Assistance Energy Providers Network FOA

    Broader source: Energy.gov [DOE]

    Transcript of the DOE Office of Indian Energy webinar about the Inter-Tribal Technical Assistance Energy Providers Network funding opportunity.

  11. Better Buildings Webinar Transcription- Financial Vehicles within an Integrated Energy Efficiency Program

    Broader source: Energy.gov [DOE]

    "Financial Vehicles Within an Integrated Energy Efficiency Program," webinar transcript from the U.S. Department of Energy's Better Buildings program.

  12. Transcript of April 3, 2013, TAP webinar titled State Energy Strategic Planning

    Broader source: Energy.gov [DOE]

    U.S. Department of Energy (DOE) Technical Assistance Program (TAP) transcript of a TAP webinar held on April 3, 2013 and dealing with state energy strategic planning.

  13. EA-1767: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    7: Finding of No Significant Impact EA-1767: Finding of No Significant Impact Virginia State Energy Program's Cephas C&D Wastes Biomass Project, Richmond, Virginia Based on the...

  14. EA-1814: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1814: Finding of No Significant Impact City of Montpelier Combined Heat and Power, and District Energy System, Montpelier, Vermont The U.S....

  15. EA-1928: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1928: Finding of No Significant Impact White-Tailed Deer Management at Brookhaven National Lab, Upton, New York Based on the information in the...

  16. EA-1665: Final Environmental Assessment and Finding of No Significant...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    5: Final Environmental Assessment and Finding of No Significant Impact EA-1665: Final Environmental Assessment and Finding of No Significant Impact Davis-Kingman Tap 69-kV...

  17. EA-1904: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    EA-1904: Finding of No Significant Impact Linac Coherent Light Source II at Stanford Linear Accelerator Laboratory, San Mateo, CA Based on the findings in the EA, DOE has...

  18. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Project, Zachary, Louisiana November 24, 2010 EA-1797: Finding of No Significant Impact Agua Caliente Solar Project in Yuma County, Arizona November 19, 2010 EA-1727: Finding of No...

  19. EIS-0365: Record of Decision and Floodplain Statement of Findings...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Record of Decision and Floodplain Statement of Findings EIS-0365: Record of Decision and Floodplain Statement of Findings Imperial-Mexicali 230-kV Transmission Lines DOE announces...

  20. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    2012 EA-1812: Finding of No Significant Impact Haxtun Wind Energy Project, Logan and Phillips Counties, CO January 3, 2012 EA-1606: Finding of No Significant Impact Proposed Use...

  1. Title 16 USC 1600 Congressional Findings | Open Energy Information

    Open Energy Info (EERE)

    600 Congressional Findings Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- StatuteStatute: Title 16 USC 1600 Congressional FindingsLegal Abstract...

  2. STEM Volunteer Training: Types of Volunteering and Where to Find...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Types of Volunteering and Where to Find Opportunities STEM Volunteer Training: Types of Volunteering and Where to Find Opportunities April 14, 2016 3:00PM to 4:00PM EDT

  3. Got a Minute? Which Higgs did we find?

    ScienceCinema (OSTI)

    Stupak, John

    2014-09-15

    Dr. John Stupak talks about the discovery of the Higgs boson. Did scientists find the Higgs boson predicted back in 1964 or did they find just one of a group of particles, with the others still to be found?

  4. EA-1809: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1809: Finding of No Significant Impact White Earth Nation Wind Energy Project II, Becker and Mahnomen Counties, MN DOE's Golden Field Office has...

  5. EA-1933: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1933: Finding of No Significant Impact Yakama Nation Drop 4 Hydropower Project, Yakama Nation Reservation, WA Based on the Final EA, DOE has...

  6. EA-1656: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1656: Finding of No Significant Impact MARET Center at Crowder College, Neosho, Missouri The U.S. Department of Energy is preparing an...

  7. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    County, Nevada June 9, 2011 EA-1770: Finding of No Significant Impact Photovoltaic Solar Project at the Durango, Colorado Site June 3, 2011 EA-1858: Finding of No Significant...

  8. EA-1833: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1833: Finding of No Significant Impact The City of El Dorado Wind Energy Project, El Dorado, Butler County, Kansas The U.S. Department of...

  9. EA-1894: Finding of No Significant Impact | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    Albeni Falls Flexible Winter Lake Operations, Bonner, Idaho As a result of the analysis in this EA, I have the following findings. I find that: 1) the FWPO is not a substantial...

  10. ULTRA-DEEP WATER ADVISORY COMMITTEE FINDINGS AND

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ULTRA-DEEP WATER ADVISORY COMMITTEE FINDINGS AND RECOMMENDATIONS 2014 ULTRA-DEEPWATER ADVISORY COMMITTEE COMMITTEE FINDINGS AND RECOMMENDATIONS i Table of Contents Research and Development Program Committee ............................................ 1 Sunset Committee .................................................................................................. 5 Advisory Committee Members ........................................................................... 7 ULTRA-DEEPWATER

  11. Rainbows and Leprechauns: Finding Gold in Partnerships (101)...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Rainbows and Leprechauns: Finding Gold in Partnerships (101) Rainbows and Leprechauns: Finding Gold in Partnerships (101) March 17, 2016 1:00PM to 2:30PM EDT

  12. Got a Minute? Which Higgs did we find?

    SciTech Connect (OSTI)

    Stupak, John

    2014-06-26

    Dr. John Stupak talks about the discovery of the Higgs boson. Did scientists find the Higgs boson predicted back in 1964 or did they find just one of a group of particles, with the others still to be found?

  13. Rainbows and Leprechauns: Finding Gold in Partnerships (101) | Department

    Energy Savers [EERE]

    of Energy Rainbows and Leprechauns: Finding Gold in Partnerships (101) Rainbows and Leprechauns: Finding Gold in Partnerships (101) March 17, 2016 1:00PM to 2:30PM EDT

  14. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    in Kahuku, O'ahu, Hawaii May 18, 2010 EA-1699: Finding of No Significant Impact PopeDouglas Third Combustor Expansion Project, Alexandria, Minnesota May 7, 2010 EA-1635: Finding...

  15. STEM Volunteer Training: Types of Volunteering and Where to Find

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Opportunities | Department of Energy Types of Volunteering and Where to Find Opportunities STEM Volunteer Training: Types of Volunteering and Where to Find Opportunities April 14, 2016 3:00PM to 4:00PM EDT

  16. Portsmouth Site Treatment Plan Director's Final Findings and Orders |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Treatment Plan Director's Final Findings and Orders Portsmouth Site Treatment Plan Director's Final Findings and Orders Portsmouth Site Treatment Plan Director's Final Findings and Orders is an agreement between the Ohio EPA and DOE approving the Site Treatment Plan and setting forth guidelines for storage and treatment of mixed wastes at the Portsmouth Facility. PDF icon Portsmouth Site Treatment Plan Director's Final Findings and Order - 1995 More Documents &

  17. FINDING TRACERS FOR SUPERNOVA PRODUCED {sup 26}Al (Journal Article) |

    Office of Scientific and Technical Information (OSTI)

    SciTech Connect FINDING TRACERS FOR SUPERNOVA PRODUCED {sup 26}Al Citation Details In-Document Search Title: FINDING TRACERS FOR SUPERNOVA PRODUCED {sup 26}Al We consider the cospatial production of elements in supernova explosions to find observationally detectable proxies for enhancement of {sup 26}Al in supernova ejecta and stellar systems. Using four progenitors, we explore a range of one-dimensional explosions at different energies and an asymmetric three-dimensional explosion. We find

  18. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Findings of No Significant Impact (FONSI) Findings of No Significant Impact (FONSI) Findings of No Significant Impact are public documents issued by a Federal agency briefly presenting the reasons why an action for which the agency has prepared an environmental assessment will not have a significant effect on the human environment and, therefore, will not require preparation of an environmental impact statement. If you have any trouble finding a specific document, please contact

  19. EA-1613: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    13: Finding of No Significant Impact EA-1613: Finding of No Significant Impact Proposed High Explosive Pressing Facility Pantex Plant, Amarillo, Texas The proposed action is to construct a new facility that would consolidate the Pantex Plant's current high explosive pressing activities at one facility. PDF icon Finding of No Significant Impact High Explosive Pressing Facility More Documents & Publications EA-1613: Final Environmental Assessment EA-1533: Finding of No Significant Impact

  20. EA-1922: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    2: Finding of No Significant Impact EA-1922: Finding of No Significant Impact Combined Power and Biomass Heating System, Fort Yukon, Alaska Based on the EA, DOE finds that providng federal funding for the Proposed Project is not a major action that constituents a significant effect on the human environment. This finding and decision is based on the consideration of DOE's NEPA implementing regulations (10 CFR Part 1021) and CEQ's criteria for significance (40 CFR 1508.27), both with regard to the

  1. EA-1406: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    06: Finding of No Significant Impact EA-1406: Finding of No Significant Impact Ground Water Compliance at the New Rifle, Colorado, UMTRA Project Site On the basis of the Environmental Assessment. which analyzed relevant environmental issues and addressed the concerns of stakeholders, DOE finds that no significant impact will result from implementing the planned action at the New Rifle site. This FONSI is documented pursuant to NEPA, the Council on Environmental Quality's "Regulations for

  2. EA-1903: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1903: Finding of No Significant Impact Kansas State University Zond Wind Energy Project, Manhattan, Kansas Based on the analysis in the EA and other considerations, DOE has decided to provide federal funding for the Proposed Project and further finds that the Proposed Project is not a major federal action that constitutes a significant effect on the human environment. The Propoesed Project will not require the preparation of an environmental impact statement.

  3. Portsmouth Integration Director's Final Findings and Order | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Integration Director's Final Findings and Order Portsmouth Integration Director's Final Findings and Order Portsmouth Integration Director's Final Findings and Order purpose is to: integrate the on-site work required for specific units to avoid duplication of effort, and efficiently perform sitewide ground water monitoring and surveillance and maintenance activities; recognize that a substantial portion of the tasks required under existing approved closure plans for certain units have

  4. EA-1548: Finding of No Significant Impact | Department of Energy

    Energy Savers [EERE]

    48: Finding of No Significant Impact EA-1548: Finding of No Significant Impact Y-12 Potable Water System Upgrade The National Nuclear Security Administration (NNSA) Y-12 Site Office proposes to upgrade its potable water system by installing two new elevated water tanks on the north side of the Y-12 National Security Complex (Y-12), and if inspection warrants, repairing or replacing existing potable water lines within the site. PDF icon Finding of No Significant Impact and Final Environmental

  5. EA-1732: Floodplain Statement of Findings | Department of Energy

    Energy Savers [EERE]

    732: Floodplain Statement of Findings EA-1732: Floodplain Statement of Findings Next Autoworks Louisiana, LLC (Formerly V-Vehicle Company) and Advanced Technology Gasoline Vehicle Manufacturing Project in Monroe, Louisiana The U.S. Department of Energy proposed action is to issue a loan to Next Autoworks Louisiana, LLC for the production of an advanced technology gasoline-powered vehicle named the V-Car. PDF icon Floodplain Statement of Findings Department of Energy Loan to Next Autoworks

  6. EA-1795: Floodplain Statement of Findings | Department of Energy

    Energy Savers [EERE]

    5: Floodplain Statement of Findings EA-1795: Floodplain Statement of Findings Loan Guarantee to Diamond Green Diesel for Construction of the Diamond Green Diesel Facility in Norco, Louisiana The Department of Energy proposed action is to issue a loan guarantee to Diamond Green Diesel, LLC to support construction of a biomass-based diesel facility adjacent to the existing Valero St. Charles Refinery in Norco, Louisiana. PDF icon Attachment Floodplain Statement of Findings for Department of Energy

  7. EA-1972: Finding of No Significant Impact | Department of Energy

    Energy Savers [EERE]

    2: Finding of No Significant Impact EA-1972: Finding of No Significant Impact Electric District 2 to Saguaro No. 2 Transmission Line Rebuild; Pinal County, Arizona Western Area Power Administration issued a finding of no significant impact for the proposed rebuild of a 35.6-mile transmission line that Western operates and maintains under an agreement with the Central Arizona Project. For more information, see the project page at: http://energy.gov/node/767356. PDF icon EA-1972-FONSI-2015.pdf

  8. EA-1979: Finding of No Significant Impact | Department of Energy

    Energy Savers [EERE]

    9: Finding of No Significant Impact EA-1979: Finding of No Significant Impact Summit Wind Farm; Summit, South Dakota Western Area Power Administration (Western) issued a finding of no significant impact for the proposed Summit Wind Farm, a proposed 99-MW wind farm south of Summit, South Dakota. The proposed wind farm would interconnect to Western's existing transmission line within the footprint of the wind farm. For more information, see the project page at: http://energy.gov/node/813914. PDF

  9. EA-2022: Finding of No Significant Impact | Department of Energy

    Energy Savers [EERE]

    Finding of No Significant Impact EA-2022: Finding of No Significant Impact Western Area Power Administration adopted and approved a final EA, prepared by the Bureau of Reclamation with Western as a cooperating agency, that assesses the potential environmental impacts of a proposal to develop a 9.4 megawatt hydroelectric project at the existing Helena Valley Pumping Plant site at Canyon Ferry Dam on the Missouri River near Helena, Montana. Western issued a finding of no significant impact for the

  10. EA-2024: Finding of No Significant Impact | Department of Energy

    Energy Savers [EERE]

    Finding of No Significant Impact EA-2024: Finding of No Significant Impact Gap Material Plutonium - Transport, Receipt, and Processing Based on the analysis in the environmental assessment, the U.S. Department of Energy, National Nuclear Security Administration (NNSA), issued this finding of no significant impact for transporting up to 900 kilograms of plutonium from foreign countries to the Savannah River Site in South Carolina for storage and processing pending final disposition. NNSA will

  11. Mound Plant Director's Final Findings and Orders, October 4, 1995

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Mound Plant Director's Final Findings and Orders, October 4, 1995 Page 1 of 16 EM Home | Regulatory Compliance | Environmental Compliance Agreements Mound Plant Director's Final Findings and Orders, October 4, 1995 BEFORE THE OHIO ENVIRONMENTAL PROTECTION AGENCY In the Matter Of: United States Department of Energy : Director's Final Mound Facility : Findings and Order P.O. Box 66 : Miamisburg, Ohio 45343-0066 : Respondent It is hereby agreed by and among the parties hereto as follows: Table of

  12. Find Funding | U.S. DOE Office of Science (SC)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Find Funding Funding Opportunities Funding Opportunities Home Grants & Contracts Support Award Search / Public Abstracts Find Funding Early Career Research Program Statement on Digital Data Management Acknowledgements of Federal Support Contact Information Office of Science U.S. Department of Energy 1000 Independence Ave., SW Washington, DC 20585 P: (202) 586-5430 Find Funding Print Text Size: A A A FeedbackShare Page Additional information on funding opportunities, including program

  13. Commercial Building Energy Asset Score: Pilot Findings and Program Update |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Pilot Findings and Program Update Commercial Building Energy Asset Score: Pilot Findings and Program Update The webinar was held on April 16, 2014, to share the findings from the 2013 Pilot and provide a program update on the Commercial Building Energy Asset Score. PDF icon Presentation More Documents & Publications Commercial Building Energy Asset Rating HES Program Update and Scoring Tool v2014 Release Commercial Building Energy Asset Score: 2013 Pilot Overview

  14. EA-1426: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1426: Finding of No Significant Impact Linac Coherent Light Source Project, Stanford Linear Accelerator Center, Menlo Park, California The DOE proposes to construct and operate a new research facility at SLAC, the Linac Coherent Light Source (LCLS), as a collaborative effort with other DOE facilities. PDF icon U.S. Department of Energy (DOE) Finding of No Significant Impact Construction and Operation of the Linac Coherent Light Source (LCLS) at the Stanford

  15. EA-1440: Finding of No Signficant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    : Finding of No Signficant Impact EA-1440: Finding of No Signficant Impact National Renewable Energy Laboratory's South Table Mountain Complex The Proposed Action is to operate the STM complex for alternative energy research with new and improved capabilities to support DOE"s mission to research, develop and transfer to industry renewable energy technologies. PDF icon Finding of No Signficant Impact for the Site-Wide Environmental Assessment of the National Renewable Energy Laboratory's

  16. EA-1471: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    71: Finding of No Significant Impact EA-1471: Finding of No Significant Impact Transportation of HEU from the Russian Federation to the Y-12 National Security Complex The Department of Energy proposes to transport highly enriched uranium from Russia to a secure storage facility in Oak Ridge, Tennessee. PDF icon Finding of No Significant Impact for the Transportation of Highly Enriched Uranium for the Russian Federation to the Y-12 National Security Complex, DOE/EA-1471 (January 2004) More

  17. EA-1772: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1772: Finding of No Significant Impact Multipurpose Haul Road within the Idaho National Laboratory Site, Idaho The proposed action consists of constructing a road for limited year-round use with the ability for trucks traveling in opposite directions to pass. PDF icon Finding of No Significant Impact for the Multipurpose Haul Road within the Idaho National Laboratory Site More Documents & Publications EA-1772: Final Environmental Assessment

  18. EA-1789: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    89: Finding of No Significant Impact EA-1789: Finding of No Significant Impact Construction and Operation of a Proposed Cellulosic Biorefinery, Alpena Prototype Biorefinery, Alpena, Michigan The Department of Energy is proposing to authorize expenditure of American Recovery and Reinvestment Act of 2009 funding to American Process, Inc. to complete final design, construction and initial operation of the Alpena Prototype Biorefinery in Alpena, Michigan. PDF icon Finding of No Significant Impact

  19. FOIA-Summary of Findings: Circumstances surrounding decisions regarding the

    Office of Environmental Management (EM)

    security clearance, access and work assignments | Department of Energy FOIA-Summary of Findings: Circumstances surrounding decisions regarding the security clearance, access and work assignments FOIA-Summary of Findings: Circumstances surrounding decisions regarding the security clearance, access and work assignments FOIA request PDF icon FOIA-Summary of Findings: Circumstances surrounding decisions regarding the security clearance, access and work assignments More Documents &

  20. Alternative Fuels Data Center: Minnesota School District Finds Cost

    Alternative Fuels and Advanced Vehicles Data Center [Office of Energy Efficiency and Renewable Energy (EERE)]

    Savings, Cold-Weather Reliability with Propane Buses Minnesota School District Finds Cost Savings, Cold-Weather Reliability with Propane Buses to someone by E-mail Share Alternative Fuels Data Center: Minnesota School District Finds Cost Savings, Cold-Weather Reliability with Propane Buses on Facebook Tweet about Alternative Fuels Data Center: Minnesota School District Finds Cost Savings, Cold-Weather Reliability with Propane Buses on Twitter Bookmark Alternative Fuels Data Center: Minnesota

  1. Department of Energy Helping Americans Find Alternative Fuel Stations |

    Office of Environmental Management (EM)

    Department of Energy Helping Americans Find Alternative Fuel Stations Department of Energy Helping Americans Find Alternative Fuel Stations May 29, 2013 - 2:14pm Addthis Helping Americans explore and adopt alternative energy sources beyond oil and gasoline has become easier. The Department of Energy's (DOE) National Renewable Energy Laboratory and DOE Clean Cities have made it a snap to find the location of alternative fuel stations across the United States by making that information

  2. EA-1148: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    48: Finding of No Significant Impact EA-1148: Finding of No Significant Impact Electrometallurgical Treatment Research and Demonstration Project in the Fuel Conditioning Facility at Argonne National Laboratory-West DOE issued a finding of no significant impact for the proposed research and demonstration of electrometallurgical technology for treating Experimental Breeder Reactor-II Spent Nuclear Fuel in the Fuel Conditioning Facility at Argonne National Laboratory-West. PDF icon

  3. EA-1458: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    8: Finding of No Significant Impact EA-1458: Finding of No Significant Impact Ground Water Compliance at the Slick Rock, Colorado, UMTRA Project Sites The U.S. Department of Energy (DOE) plans to implement ground water compliance strategies for two Uranium Mill Tailings Remedial Action (UMTRA) Project sites near Slick Rock. Colorado. PDF icon Finding of No Significant Impact Ground Water Compliance at the Slick Rock, Colorado, UMTRA Project Sites More Documents & Publications EA-1458:

  4. EA-1495: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    5: Finding of No Significant Impact EA-1495: Finding of No Significant Impact USEC Inc. American Centrifuge Lead Cascade Facility at Piketon, Ohio The purpose of NRC's proposed action is to authorize possession and use of source and special nuclear material at the Lead Cascade in Piketon, Ohio. PDF icon Finding of No Significant Impact for the United States Enrichment Corporation Incorporated, American Centrifuge Lead Cascade Facility at Piketon, Ohio More Documents & Publications EIS-0468:

  5. EA-1499: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    Finding of No Significant Impact EA-1499: Finding of No Significant Impact Radiological/Nuclear Countermeasures Test and Evaluation Complex, Nevada Test Site The National Nuclear Security Administration (NNSA) has prepared an Environmental Assessment (DOE/EA-1499) (EA) which analyzes the potential environmental effects of the proposed Rad/NucCTEC at the NTS. NNSA finds that the proposed action would not constitute a major federal action significantly affecting the quality of the human

  6. EA-1795: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    Finding of No Significant Impact EA-1795: Finding of No Significant Impact Loan Guarantee to Diamond Green Diesel for Construction of the Diamond Green Diesel Facility in Norco, Louisiana The Department of Energy conducted an environmental assessment that analyzed the potential environmental impacts associated with the construction and operation of a biomass-based diesel facility adjacent to the existing Valero St. Charles Refinery in Norco, Louisiana. PDF icon Finding of No Significant Impact

  7. EA-1796: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    6: Finding of No Significant Impact EA-1796: Finding of No Significant Impact Loan Guarantee to Sempra Generation for Construction of the Mesquite Solar Energy Project; Maricopa County, Arizona DOE (Loan Programs) issued a FONSI for the issuance of a loan guarantee for construction of the proposed Mesquite Solar Energy Project. PDF icon Finding of No Significant Impact Department of Energy Loan Guarantee to Sempra Generation for the Mesquite Solar Energy Facility near Gillespie, Arizona,

  8. EA-1797: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    7: Finding of No Significant Impact EA-1797: Finding of No Significant Impact Agua Caliente Solar Project in Yuma County, Arizona The Department of Energy has conducted an environmental assessment that analyzed the reasonably foreseeable environmental impacts associated with the 290 Megawatt gross output photovoltaic solar power project and associated interconnection transmission line proposed by Agua Caliente Solar, LLC in Yuma County, Arizona. PDF icon Finding of No Significant Impact

  9. EA-1801: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    1: Finding of No Significant Impact EA-1801: Finding of No Significant Impact Granite Reliable Power Wind Project, Coos County, New Hampshire The Department of Energy is adopting an environmental assessment completed by the U.S. Army Corps of Engineers in June 2010 that analyzed the potential environmental impacts associated with the construction and operation by Granite Reliable Power, LLC of the Granite Reliable Power Wind Park located in central Coos County, New Hampshire. PDF icon Finding of

  10. EA-1812: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    2: Finding of No Significant Impact EA-1812: Finding of No Significant Impact Haxtun Wind Energy Project, Logan and Phillips Counties, Colorado DOE has issued a Finding of No Significant Impact (FONSI) based on the information in the Final EA, FONSI, and the Supplement Analysis (SA) prepared to address changes in the design and operating parameters of the NECO (formerly Haxtun) Wind Farm Project ("original proposed project") in Logan and Phillips Counties, Colorado. In January 2012,

  11. EA-1859: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    Finding of No Significant Impact EA-1859: Finding of No Significant Impact Kirkwood Community College - Kirkwood Wind Turbine Project, Cedar Rapids, Iowa The Iowa State Energy Office is proposing to provide $1,050,000 of its State Energy Program funds to Kirkwood Community College located in Cedar Rapids, Iowa, which would use these funds to purchase equipment for one 2.5-megawatt wind turbine. PDF icon Finding of No Significant Impact Kirkwood Community College Wind Turbin Project More

  12. EA-1948: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    Finding of No Significant Impact EA-1948: Finding of No Significant Impact Gila-North Gila Transmission Line Rebuild and Upgrade Project, Yuma County, Arizona DOE's Western Area Power Administration issued a finding of no significant impact for a proposal to rebuild and upgrade two parallel 4.8-mile transmission lines between the Gila and North Gila Substations, to expand its right-of-way, and to grant Arizona Power Service Company the right to cross Western's Gila Substation with its North

  13. EA-1836: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    to facilitiate installation and operations of a high-efficiency natural-gas-fired cogeneration facility - would result in no significant adverse impacts. Finding of No...

  14. Findings of No Significant Impact (FONSI) | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    Ridge Reservation for the biological control of the emerald ash borer, a nonnative invasive beetle. May 1, 2015 EA-1796: Finding of No Significant Impact Loan Guarantee to...

  15. Main Page Main Page: Find energy information and data. Share...

    Open Energy Info (EERE)

    Main Page Main Page: Find energy information and data. Share knowledge. Connect with people. energy datasets energy community Main Page energy information open data 0...

  16. EA-1762: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of No Significant Impact EA-1762: Finding of No Significant Impact Wellford Landfill Methane and Greenhouse Gas to Energy Project, Spartanburg County, South Carolina The...

  17. EA-1770: Finding of No Significant Impact | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    of the National Environmental Policy Act (NEPA). Finding of No Significant Impact Photovoltaic Solar Project at the Durango, Colorado, Disposal Site, La Plata County, DOEEA 1770...

  18. EA-1683: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    Impact EA-1683: Finding of No Significant Impact Abengoa Solar Inc., the Solana Thermal Electric Power Project near Gila Bend, Arizona The Department of Energy has...

  19. ADOPTION OF ENVIRONMENTAL ASSESSMENT and FINDING OF NO SIGNIFICANT...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ADOPTION OF ENVIRONMENTAL ASSESSMENT and FINDING OF NO SIGNIFICANT IMPACT; DETERMINATION ... ADOPTION: DOE hereby adopts as a DOE Environmental Assessment, DOEEA-1868, the National ...

  20. FOIA-Summary of Findings: Circumstances surrounding decisions...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    the security clearance, access and work assignments FOIA-Summary of Findings: Circumstances surrounding decisions regarding the security clearance, access and work assignments ...

  1. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of the Department of Energy, Waste Isolation Pilot Plant (WIPP) in Eddy County, New Mexico January 13, 2010 EA-1651: Finding of No Significant Impact U-233 Material...

  2. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    and Heating Facilities at the Savannah River Site, South Carolina June 11, 2008 EA-1613: Finding of No Significant Impact Proposed High Explosive Pressing Facility Pantex...

  3. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of No Significant Impact Proposed Gas Main and Distribution System Upgrade, Pantex Plant, Amarillo, Texas August 26, 2005 EA-1542: Finding of No Significant Impact Burleigh County...

  4. Microsoft Word - Summary of RCA findings and lessons learned...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Work Planning and Scheduling System project Root cause analysis findings and lessons learned Summary BPA stopped the Work Planning and Scheduling System project in February 2012...

  5. EA-1777: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Significant Impact EA-1777: Finding of No Significant Impact Lincoln Electric's Wind Energy Project in Euclid, OH DOE prepared an environmental assessment analyzing the potential...

  6. PPPL physicists find clue to formation of magnetic fields around...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    results, in the range in which they are done, strongly suggest that such dynamo action is possible." These findings might lead to greater understanding of the behavior of...

  7. EA-1907: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Impact EA-1907: Finding of No Significant Impact Western Plains Energy, LLC Biogas Anaerobic Digester Facility, Oakley, Kansas Based on the analysis in the Final EA and...

  8. EA-1685: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of No Significant Impact EA-1685: Finding of No Significant Impact Experimental Coal Flow Program at Clemson University The Western Area Power Administration (Western) is the...

  9. EA-1921: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact Silver Peak Area Geothermal Exploration Project, Esmeralda County, NV Based on the evaluations in the final EA and subject to the mitigations...

  10. EA-1974: Finding of No Significant Impact and Mitigation Action...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Bonneville Power Administration issued a finding of no significant impact (FONSI) for the proposed restoration of a tidal marsh in the Columbia River Estuary, near Astoria in ...

  11. Finding of No Significant Impact, Federal Register, 73 FR 2017...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    73 FR 2017, January 11, 2008 Document details the finding of no significant impact of the Energy-Efficient Performance Requirements for New Federal Commercial and Residential...

  12. EA-1539: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Significant Impact EA-1539: Finding of No Significant Impact North Area Right-of-Way Maintenance, California Western Area Power Administration proposes to revise its operation and...

  13. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    EA-1754: Finding of No Significant Impact Public Service Company of New Mexico, Photovoltaic Plus Battery for Simultaneous Voltage Smoothing and Peak Shifting Project,...

  14. Mound Plant Director's Final Findings and Orders, October 4,...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Director's Final Findings and Orders State Ohio Agreement Type Compliance Agreement Legal Driver(s) FFCAct Scope Summary Approve the Compliance Plan Volume of the amended PSTP,...

  15. EA-1837: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact Performance Verification Laboratory, Morgantown, West Virginia Based on the analyses in the environmental assessment, DOE determined that its...

  16. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Uranium July 1, 2009 EA-1645: Finding of No Significant Impact Loan Guarantee for Sage Electrochromics SageGlass High Volume Manufacturing Facility in Faribault, MN June 26,...

  17. CLASS DEVIATION FINDINGS AND DETERMINATION TO IMPLEMENT AN APPROPRIATI...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    PROVISION RELATED TO INTERNAL CONFIDENTIALITY AGREEMENTS AND THE REPORTING OF FRAUD, WASTE, AND ABUSE Findings I. Section 743 of Division E, Title VII of the Consolidated...

  18. EA-1696: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    EA-1696: Finding of No Significant Impact Pantex Renewable Energy Project, Amarillo, Texas The NNSA has prepared an Environmental Assessment to analyze the potential...

  19. Innovative Financing Solutions: Finding Money for Your Energy Efficiency Projects

    Broader source: Energy.gov [DOE]

    Provides an overview of how to find funding for your energy efficiency projects focusing on l-p agreements. Author: Energy Star

  20. *** CANCELED *** SCIENCE ON SATURDAY- "Finding a Needle in A...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ON SATURDAY- "Finding a Needle in A (Genomic) Haystack or How Can Computers Help Cure Cancer" Professor Olga G. Troyanskaya Lewis-Sigler Institute for Integrative Genomics &...

  1. EA-2014: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    No Significant Impact EA-2014: Finding of No Significant Impact Y-12 National Security Complex Emergency Operations Center; Oak Ridge, TN Based upon the EA, the National Nuclear...

  2. EA-1719: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Impact EA-1719: Finding of No Significant Impact Novolyte Technologies, Inc. Electric Drive Vehicle Battery and Component Manufacturing Initiative Project, Zachary, Louisiana DOE...

  3. EA-1723: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Impact EA-1723: Finding of No Significant Impact General Motors LLC Electric Drive Vehicle Battery and Component Manufacturing Initiative Application, White Marsh,...

  4. Using regulations.gov to find dockets and documents

    Broader source: Energy.gov (indexed) [DOE]

    regulations.gov to find dockets and documents Regulations.gov is a web-based repository of Federal regulatory documents. Publicly accessible documents associated with DOE Appliance ...

  5. EA-1927: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of No Significant Impact EA-1927: Finding of No Significant Impact Paducah Gaseous Diffusion Plant Potential Land and Facilities Transfers; McCracken County, Kentucky DOE's...

  6. EA-1882: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Range Environmental Assessment, DOEEA-1882 (March 2011) More Documents & Publications EA-1822: Final Environmental Assessment EA-1984: Finding of No Significant Impact EA-1555...

  7. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    No Significant Impact Sunflower Wind Project, Hebron, North Dakota September 3, 2014 EA-1984: Finding of No Significant Impact Disposition of Five Signature Properties at Idaho...

  8. Oregon 401 Evaluation and Findings Checklist | Open Energy Information

    Open Energy Info (EERE)

    401 Evaluation and Findings Checklist Jump to: navigation, search OpenEI Reference LibraryAdd to library PermittingRegulatory Guidance - Checklist: Oregon 401 Evaluation and...

  9. EA-1864: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Impact EA-1864: Finding of No Significant Impact Electrical District 5 - Palo Verde Hub Project The U.S. Department of Energy (DOE) Western Area Power Administration...

  10. EA-1212: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    Finding of No Significant Impact EA-1212: Finding of No Significant Impact Lease of Land for the Development of a Research Park at Los Alamos National Laboratory The U.S.. Department of Energy finds that there would be no significant impact from proceeding with its proposal to lease to the County of Los Alamos a 60-acre tract located at Technical Area 3 of Los Alamos National Laboratory, for their development and use as a research park. This finding is based on the EA that analyzes the

  11. EA-1700: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    LBNLUniversity of California Berkeley programs that focus on computational and computer science research in a new facility on the LBNL site. PDF icon Finding of No Significant...

  12. EA-1679: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    9: Finding of No Significant Impact EA-1679: Finding of No Significant Impact Grand Coulee's Third Powerplant 500-kV Transmission Line Replacement Project, Grant and Okanogon Counties, Washington PDF icon EA-1679-FONSI-2011.pdf More Documents & Publications EA-1679: Mitigation Action Plan EA-1679: Revision Sheet for Final Environmental Assessment EA-1679: Preliminary Environmental Assessment

  13. EA-1792: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1792: Finding of No Significant Impact University of Maine's Deepwater Offshore Floating Wind Turbine Testing and Demonstration Project, Gulf of Maine PDF icon EA-1792-FONSI-2011.pdf More Documents & Publications EA-1792: Draft Environmental Assessment EA-1792-S1: Draft Supplemental Environmental Assessment EA-1792-S1: Final

  14. The activity of CouR, a MarR family transcriptional regulator, is modulated

    Office of Scientific and Technical Information (OSTI)

    through a novel molecular mechanism (Journal Article) | SciTech Connect The activity of CouR, a MarR family transcriptional regulator, is modulated through a novel molecular mechanism Citation Details In-Document Search Title: The activity of CouR, a MarR family transcriptional regulator, is modulated through a novel molecular mechanism CouR, a MarR-type transcriptional repressor, regulates the cou genes, encoding p-hydroxycinnamate catabolism in the soil bacterium Rhodococcus jostii RHA1.

  15. How to Find Ames Laboratory | The Ames Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    How to Find Ames Laboratory Finding the Ames Laboratory The Ames Laboratory has it's main administrative offices in the Technical and Administrative Services Facility (TASF) building on the Iowa State University Campus. For directions, please use the GPS coordinates 42.0305° N, 93.6482° W. For directions from the Des Moines Airport please use the link with the following directions: https://goo.gl/maps/c6SOK Finding the Ames Laboratory on the ISU Campus Image Click on the map to download a

  16. EV Everywhere: Find Electric Vehicle Models | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Find Electric Vehicle Models EV Everywhere: Find Electric Vehicle Models Search Car: Year: -- ALL -- Make: -- ALL -- Market Class: -- ALL -- All-Electric Range: Min -- ALL -- 10 miles 20 miles 30 miles 40 miles 50 miles 60 miles 70 miles 80 miles 90 miles 100 miles 110 miles 120 miles 130+ miles Gasoline Back-Up Available: -- ALL -- No Yes Reset To find out if a plug-in electric vehicle (EV) will work for you, use the menus to the left to sort the available EV models on the market by year, make,

  17. Resources for Handling Transcripts | U.S. DOE Office of Science...

    Office of Science (SC) Website

    The transcript PDF does not have to be a color copy. If applicant does not have a personal scanner or need assistance formatting the file, applicant should consider visiting a...

  18. Transcript of April 11, 2013, TAP webinar titled Financing Energy Upgrades for K-12 School Districts

    Broader source: Energy.gov [DOE]

    U.S. Department of Energy (DOE) Technical Assistance Program (TAP) transcript of a TAP webinar held on April 11, 2013 and dealing with how to finance energy efficiency upgrades for K-12 school districts.

  19. DOE ZERH Webinar: Tour of Zero, Helping Customers Find Better...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Are you interested in helping homebuyers find a better home at a lower cost? The DOE Tour of ... This national campaign is where you come in. DOE needs Innovation partners vested in ...

  20. EA-1760: Finding of No Significant Impact | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    of a commercial-scale plant to produce intermediate anode material for high-performance lithium-ion batteries would result in no significant adverse impacts. Finding of No...

  1. EA-1711: Finding of No Significant Impact | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    America, Inc to construct and operate a high-volume manufacturing plant to make advanced lithium-ion cells and batteries, would result in no significant adverse impacts. Finding...

  2. Accident Investigation Board (AIB) findings about the drum breach...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    findings about the drum breach at WIPP WHEN: Apr 23, 2015 5:30 PM - 7:00 PM WHERE: Fuller Lodge 2132 Central Avenue, Los Alamos CATEGORY: Community TYPE: Meeting INTERNAL:...

  3. Finding Large Aperture Fractures in Geothermal Resource Areas...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    A Three-Component Long-Offset Surface Seismic Survey, PSInSAR, and Kinematic Analysis Finding Large Aperture Fractures in Geothermal Resource Areas Using A Three-Component...

  4. EA-1888: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of No Significant Impact EA-1888: Finding of No Significant Impact Old Town Fuel and Fiber Proposed Demonstration-Scale Integrated Biorefinery in Old Town, MN DOE has determined...

  5. EA-1964: FInding of No Significant Impact | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    13, DOE adopted the NSF EA and issued a finding of no significant impact regarding NEON Project research activities on the Oak Ridge Reservation. EA-1964-FONSI-2013.pdf More...

  6. Findings of No Significant Impact (FONSI) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Winnebago County, Illinois December 2, 2011 EA-1697: Finding of No Significant Impact Right-of-Way Maintenance in the San Joaquin Valley, California November 10, 2011 EA-1862:...

  7. Floodplain and Wetland Assessment and Statement of Findings

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    U.S. DEPARTMENT OF ENERGY GOLDEN FIELD OFFICE Floodplain and Wetlands Assessment and Statement of Findings for Construction of a Second Full Service Access Road over Lena Gulch ...

  8. Impact of the N-Terminal Domain of STAT3 in STAT3-Dependent Transcriptional

    Office of Scientific and Technical Information (OSTI)

    Activity (Journal Article) | SciTech Connect Impact of the N-Terminal Domain of STAT3 in STAT3-Dependent Transcriptional Activity Citation Details In-Document Search Title: Impact of the N-Terminal Domain of STAT3 in STAT3-Dependent Transcriptional Activity Authors: Hu, Tiancen ; Yeh, Jennifer E. ; Pinello, Luca ; Jacob, Jaison ; Chakravarthy, Srinivas ; Yuan, Guo-Cheng ; Chopra, Rajiv ; Frank, David A. [1] ; DFCI) [2] ; Harvard) [2] ; IIT) [2] ; BWH) [2] + Show Author Affiliations

  9. Moving Industry Forward: Finding the Environmental Opportunity in Biochar

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Moving Industry Forward: Finding the Environmental Opportunity in Biochar Moving Industry Forward: Finding the Environmental Opportunity in Biochar Print Thursday, 12 September 2013 08:41 The Environmental Protection Agency (EPA) has been investigating biofuels for several years now as part of its mission of moving the nation toward a cleaner energy future. Recently, EPA scientists have refocused their research on a promising biofuel byproduct-biochar, which is the solid material that's left

  10. Neutrons find "missing" magnetism of plutonium

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Neutrons find "missing" magnetism of plutonium Neutrons find "missing" magnetism of plutonium Groundbreaking work at two Department of Energy national laboratories has confirmed plutonium's magnetism, which scientists have long theorized but have never been able to experimentally observe. July 10, 2015 Doug Abernathy, left, ARCS instrument scientist at Oak Ridge National Laboratory, and Marc Janoschek, Los Alamos National Laboratory, prepare their sample for experiments at

  11. Study finds surprising variability in shape of Van Allen Belts

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Study finds surprising variability in shape of Van Allen Belts Study finds surprising variability in shape of Van Allen Belts Understanding the shape and size of the belts, which shrink and swell in response to magnetic storms coming from the sun, is crucial for protecting our technology in space. February 23, 2016 1. The traditional idea of the radiation belts includes a larger, more dynamic outer belt and a smaller, more stable inner belt with an empty slot region separating the two. However,

  12. EA-1611: Finding of No Significant Impact | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    finding of no significant impact (FONSI) for the proposed Colorado Highlands Wind Project.The FONSI describes the information Western used to determine that Western's proposal to allow interconnection of the Project will not have a significant impact on the human environment. PDF icon EA-1611-FONSI-2014.pdf More Documents & Publications EA-1611-S1: Finding of No Significant Impact EA-1611: Final Environmental Assessment EA-1611: Mitigation Action Plan

  13. EA-1740: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1740: Finding of No Significant Impact Thermal Energy Corporation Combined Heat and Power Project Houston, Texas DOE completed the Final Environmental Assessment (EA) for the Thermal Energy Corporation Combined Heat and Power Project, Houston, Texas (DOE/EA-1740). Based on the analyses in the EA, DOE determined that its proposed action-awarding a federal grant to the Thermal Energy Corporation (TECO) to facilitate installationg of a combined heat and power

  14. CREED: Helping Colorado Cleantech Startups Find the Money - News Releases |

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    NREL CREED: Helping Colorado Cleantech Startups Find the Money January 19, 2012 The Colorado Center for Renewable Energy Economic Development (CREED) at the U.S. Department of Energy's National Renewable Energy Laboratory (NREL) invites cleantech entrepreneurs to attend the next event in its Entrepreneur Series. The Jan. 26 class, "Financing Your Cleantech Startup," will provide cleantech entrepreneurs with an overview of how to find capital for a startup. "If you are in

  15. EA-1868: Finding of No Significant Impact | Department of Energy

    Energy Savers [EERE]

    68: Finding of No Significant Impact EA-1868: Finding of No Significant Impact Manhattan Project Sites (Hanford, Washington; Los Alamos, New Mexico, Oak Ridge, Tennessee) The National Park Service (NPS) issued an Environmental Assessment (September 2010) in consultation with DOE for a proposed action of recommending that Congress authorize an innovative Manhattan Project National Historical Park. Based on the information presented in DOE/EA-1868, there would be no significant impacts to the

  16. EA-1915: Finding of No Significant Impact | Department of Energy

    Energy Savers [EERE]

    Finding of No Significant Impact EA-1915: Finding of No Significant Impact Proposed Conveyance of Land at the Hanford Site, Richland, Washington Based on the analysis presented in the Final EA, which considered comments received on the Draft EA and the commitments specified in the Mitigation Action Plan, DOE has determined that the Proposed Action will not constitute a major Federal action significantly affecting the quality of the human environment within the meaning of NEPA. Therefore, the

  17. EA-1983: Finding of No Significant Impact | Department of Energy

    Energy Savers [EERE]

    3: Finding of No Significant Impact EA-1983: Finding of No Significant Impact Sabine Pass Liquefaction Expansion Project, Cameron Parish, Louisiana DOE adopted an EA prepared by Federal Energy Regulatory Commission (FERC), with DOE as a cooperating agency, for a proposal to expand the Sabine Pass Liquefied Natural Gas Terminal and extend an associated pipeline system. DOE issued a FONSI. PDF icon EA-1983-FONSI-2015.pdf More Documents & Publications Summary of LNG Export Applications of the

  18. DOE issues Finding of No Significant Impact for the Environmental

    Energy Savers [EERE]

    Assessment on Resumption of Transient Testing of Nuclear Fuels and Materials at Idaho National Laboratory | Department of Energy issues Finding of No Significant Impact for the Environmental Assessment on Resumption of Transient Testing of Nuclear Fuels and Materials at Idaho National Laboratory DOE issues Finding of No Significant Impact for the Environmental Assessment on Resumption of Transient Testing of Nuclear Fuels and Materials at Idaho National Laboratory February 28, 2014 - 12:00pm

  19. Two Independent Assessments Find the Department of Energy's Yucca Mountain

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Project is on Track | Department of Energy Independent Assessments Find the Department of Energy's Yucca Mountain Project is on Track Two Independent Assessments Find the Department of Energy's Yucca Mountain Project is on Track December 13, 2007 - 4:44pm Addthis WASHINGTON, DC - The U.S. Department of Energy (DOE) Director of the Office of Civilian Radioactive Waste Management (OCRWM) today released two independent assessments addressing areas critical to the overall success of the Yucca

  20. DOE Issues Finding of No Significant Impact and Final Environmental

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Assessment on Radiological Response Training Range DOE Issues Finding of No Significant Impact and Final Environmental Assessment on Radiological Response Training Range October 14, 2010 Media contact: Tim Jackson (208) 526-8484 The U.S. Department of Energy has issued a Finding of No Significant Impact stemming from an environmental assessment it performed to examine the potential environmental impacts of operating a Radiological Response Training Range at Idaho National Laboratory. At the

  1. DOE Issues Finding of No Significant Impact and Final Environmental

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Assessment on Road Project U.S. DEPARTMENT OF ENERGY IDAHO FALLS, IDAHO, 83403 News Media Contact: Tim Jackson (208) 526-8484 For Immediate Release Thursday August 12, 2010 DOE Issues Finding of No Significant Impact and Final Environmental Assessment on Road Project The U.S. Department of Energy has issued a Finding of No Significant Impact stemming from an environmental assessment it performed to examine the potential environmental impacts of building an internal road to safely ship

  2. Finding of No Significant Impact Issued on Environmental Assessment for

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Remote-handled Waste Disposition Project at DOE�s Idaho Site Finding of No Significant Impact Issued on Environmental Assessment for Remote-handled Waste Disposition Project at DOE�s Idaho Site The U.S. Department of Energy has issued a Finding of No Significant Impact (FONSI) on the Final Environmental Assessment for the Remote-handled Waste Disposition Project. The environmental assessment analyzed potential environmental impacts of processing for disposition approximately 322 cubic

  3. Finding the Right Filling Station for Alternative Vehicles Now Easier

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Finding the Right Filling Station for Alternative Vehicles Now Easier For more information contact: e:mail: Public Affairs Golden, Colo., May 5, 1999 — A new online interactive computer program is taking the guesswork out of finding the fuel needed by the thousands of alternative vehicles on the road today in the United States. The program, called the Alternative Fuel Refueling Station Locator, was developed by the U.S. Department of Energy's (DOE) Alternative Fuels Data Center (AFDC). The AFDC

  4. Jefferson Lab finds its man Mont (Inside Business) | Jefferson Lab

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    https://www.jlab.org/news/articles/jefferson-lab-finds-its-man-mont-inside-business Jefferson Lab finds its man Mont Hugh Montgomery Hugh Montgomery, a British nuclear physicist selected from more than 50 candidates, takes over as lab director Sept. 2. By Michael Schwartz, Inside Business April 14, 2008 Replacing the head of a world-renowned nuclear physics facility is no easy feat. When Christoph Leemann announced his desire to retire in 2007 as director of the Department of Energy's Thomas

  5. Curiosity rover finds evidence of Mars' primitive continental crust

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Curiosity rover finds evidence of Mars' primitive continental crust Curiosity rover finds evidence of Mars' primitive continental crust This is the first discovery of a potential "continental crust" on Mars. July 13, 2015 Igneous clast named Harrison embedded in a conglomerate rock in Gale crater, Mars, shows elongated light-toned feldspar crystals. The mosaic merges an image from Mastcam with higher-resolution images from ChemCam's Remote Micro-Imager. Credit:

  6. Energy Department Report Finds Major Potential to Increase Clean

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Hydroelectric Power | Department of Energy Report Finds Major Potential to Increase Clean Hydroelectric Power Energy Department Report Finds Major Potential to Increase Clean Hydroelectric Power April 17, 2012 - 12:39pm Addthis Washington, D.C. -- As part of President Obama's all-out, all-of-the-above energy strategy, the Energy Department today released a renewable energy resource assessment detailing the potential to develop electric power generation at existing dams across the United

  7. EA-0984: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    84: Finding of No Significant Impact EA-0984: Finding of No Significant Impact Deactivation of the N Reactor Facilities, Richland, Washington Based on the analysis in the EA, and and considering preapproval comments from the National Park Service, the State of Washington, and the Yakama Indian Nation, DOE has determined that the proposed action is not a major federal action significantly affecting the quality of the human environment within the meaning of the NEPA. Therefore, the preparation of

  8. EA-1157: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    57: Finding of No Significant Impact EA-1157: Finding of No Significant Impact Methyl Chloride via Oxyhydrochlorination of Methane: A Building Black for Chemicals and Fuels from Natural Gas The proposed Federal action, to provide cost-shared financial assistance for demonstration of the OHC process in an engineering scale facility, does not constitute a major Federal action that would significantly affect the quality of the human environment as defined by NEPA. This conclusion is based on the

  9. EA-1183: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    83: Finding of No Significant Impact EA-1183: Finding of No Significant Impact Coal-fired Diesel Generator University of Alaska, Fairbanks, Alaska Based on analysis in the EA, DOE has determined that the proposed Federal action, to provide cost-shared financial assistance for demonstrating the performance of a coal-fired generator at the University of Alaska, Fairbanks, does not constitute a major Federal action that would significantly affect the quality of the human environment as defined by

  10. EA-1628: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    8: Finding of No Significant Impact EA-1628: Finding of No Significant Impact Construction and Operation of a Proposed Lignocellulosic Biorefinery, POET Project LIBERTY, LLC, Emmetsburg, Iowa The Department of Energy proposes to provide financial assistance (the Proposed Action) to POET Project LIBERTY, LLC (POET) for the construction and operation of the lignocellulosic ethanol production facility (Project LIBERTY) near the City of Emmetsburg, Iowa. PDF icon Mitigation Action Plan for the

  11. EA-1664: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    4: Finding of No Significant Impact EA-1664: Finding of No Significant Impact Energy Conservation Standards: Energy Conservation Standards for Fluorescent and Incandescent Lamps The U.S. Department of Energy has determined that the adoption of energy conservation standards for General Service Fluorescent Lamps and Incandescent Reflector Lamps as described in the final rule titled the "energy Conservation Program: Energy Conservation Standards for General Service Fluorescent Lamps and

  12. EA-1673: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    3: Finding of No Significant Impact EA-1673: Finding of No Significant Impact Energy Conservation Program for Certain Industrial Equipment: Energy Conservation Standards and Test Procedures for Commercial Heating, Air-Conditioning, and Water-Heating Equipment Based upon the environmental assessment, DOE has determined that the adoption of the amended energy conservation standards for commercial packaged boilers and new energy conservation standards for water-cooled and evaporatively-cooled

  13. EA-1678: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1678: Finding of No Significant Impact Loan to Nissan North America, Inc., for Advanced Technology Electric Vehicle Manufacturing Project in Smyrna, Tennessee The Department of Energy conducted an environmental assessment that analyzed the potential environmental impacts associated with the Nissan North America Inc., Electric Vehicle Production Project, which would include construction of an approximately 1.3 million square foot lithium-ion battery plant and

  14. EA-1716: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    6: Finding of No Significant Impact EA-1716: Finding of No Significant Impact Honeywell International, Inc., Electric Drive Vehicle Battery and Component Manufacturing Initiative Project Massac County, Illinois Based on the analyses in the Environmental Assessment (EA), DOE determined that its proposed action-awarding a federal greant to Honeywell International Inc. (Honeywell) for the construction of a manufacturing plant to produce a critical battery material, lithium hexafluorophosphate

  15. EA-1747: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    47: Finding of No Significant Impact EA-1747: Finding of No Significant Impact Rocky Flats Surface Water Configuration, Jefferson County, Colorado Surface Water Configuration Project at the Rocky Flats Site, Colorado PDF icon FONSI-Rocky_Flats_Surface_Water_Configuration.pdf More Documents & Publications EA-1747: Final Environmental Assessment EA-1093: Final Environmental Assessment Long-Term Surveillance and Maintenance at Rocky Flats: Early Experiences and Lessons Learned

  16. EA-1786: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    6: Finding of No Significant Impact EA-1786: Finding of No Significant Impact Algenol Integrated Biorefinery for Producing Ethanol from Hybrid Algae Under Recovery Act funding, Algenol will design, construct and operate a pilot-scale integrated biorefinery which would produce ethanol from carbon dioxide and seawater using hybrid blue-green algae. PDF icon Algenol Integrated Biorefinery for Producing Ethanol from Hybrid Algae More Documents & Publications EA-1786: Final Environmental

  17. Moving Industry Forward: Finding the Environmental Opportunity in Biochar

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Moving Industry Forward: Finding the Environmental Opportunity in Biochar Moving Industry Forward: Finding the Environmental Opportunity in Biochar Print Thursday, 12 September 2013 08:41 The Environmental Protection Agency (EPA) has been investigating biofuels for several years now as part of its mission of moving the nation toward a cleaner energy future. Recently, EPA scientists have refocused their research on a promising biofuel byproduct-biochar, which is the solid material that's left

  18. EA-2036: Finding of No Significant Impact | Department of Energy

    Energy Savers [EERE]

    Finding of No Significant Impact EA-2036: Finding of No Significant Impact Sabine Pass Liquefaction Project (design optimization); Cameron Parish, Louisiana In January 2014, the Federal Energy Regulatory Commission (FERC) issued an EA to analyze the potential environmental impacts associated with an application to amend FERC's April 2012 authorization of the Sabine Pass Liquefaction Project in order to optimize its design and operation. In April 2015, Sabine Pass Liquefaction, LLC, and Sabine

  19. Find Financing for Energy Efficiency Upgrades | Department of Energy

    Energy Savers [EERE]

    Find Financing for Energy Efficiency Upgrades Find Financing for Energy Efficiency Upgrades Photo of a block in lower downtown Denver that is part of Living City Block, a DOE Commercial Building Partnerships participant. Financing need not be a barrier to improving energy efficiency in your commercial building. There are federal, state, and local and tax incentives for energy efficiency upgrades, and other financial opportunities to help you meet your energy goals. The U.S. Department of

  20. Find Product Categories Covered by Efficiency Programs | Department of

    Office of Environmental Management (EM)

    Energy Products & Technologies » Energy-Efficient Products » Find Product Categories Covered by Efficiency Programs Find Product Categories Covered by Efficiency Programs Legend ★ ENERGY STAR △ FEMP Designated O EPEAT ♦ Low Standby Power X WaterSense Federal agencies are required to purchase energy-efficient products. To help buyers meet these requirements, the Federal Energy Management Program (FEMP) maintains acquisition guidance for the following product categories, which are

  1. EA-1970: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1970: Finding of No Significant Impact Fishermen's Atlantic City Windfarm, LLC Offshore Wind Demonstration Project, offshore Atlantic City, New Jersey Based upon the EA, DOE has determined that authorizing the expenditure of federal funding to the Fishermen's Atlantic City Windfarm, LLC to construct and operate up to six wind turbine generators, for an offshore wind demonstration project, approximately 2.8 nautical miles off the coast of Atlantic City, New

  2. EA-1985: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    85: Finding of No Significant Impact EA-1985: Finding of No Significant Impact Virginia Offshore Wind Technology Advancement Project on the Atlantic Outer Continental Shelf Offshore Virginia DOE is proposing to fund Virginia Electric and Power Company's Virginia Offshore Wind Technology Advancement Project (VOWTAP). The proposed VOWTAP project consists of design, construction and operation of a 12 megawatt offshore wind facility located approximately 24 nautical miles off the coast of Virginia

  3. EA-2001: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-2001: Finding of No Significant Impact Energy Efficiency Design Standards: New Federal Commercial and Multi-Family High-Rise Residential Buildings and New Federal Low-Rise Residential Buildings Based upon the EA, DOE has determined that revising the Federal building energy efficiency standards for commercial and multi-family high-rise residential buildings to ASHRAE 90.1-2013 would not be a major Federal action significantly affecting the quality of the human

  4. EA-2004: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-2004: Finding of No Significant Impact The Seneca Nation Wind Turbine Project, Cattaraugus Territory, Erie County, New York Based upon the EA, DOE has determined that authorizing the expenditure of federal funding to the Seneca Nation of Indians to design, permit, and construct up to a 2.0 megawatt wind turbine on Tribal common lands in the Cattaraugus Territory, New York would not be a major Federal action significantly affecting the quality of the human

  5. EA-2017: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-2017: Finding of No Significant Impact Braddock Locks and Dam Hydro Electric Project Based upon the review of the EA prepared by the Federal Energy Regulatory Commission (FERC) and adopted by DOE pursuant to the requirements of the National Environmental Policy Act (NEPA), DOE has determined that authorizing the expenditure of federal funding to Hydro Green Energy, LLC to fabricate, install, and operate one interchangeable Modular Bulb Turbine (MBT), which

  6. EA-0921: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    21: Finding of No Significant Impact EA-0921: Finding of No Significant Impact Ambulatory Research and Education Center, Oregon Health Sciences University Based on the analysis in the EA, DOE has determined that the proposed construction and operation of the Ambulatory Research and Education Center at Oregon Health Sciences University does not constitute a major Federal action significantly affecting the quality of the human environment within the meaning of NEPA. Therefore, an environmental

  7. EA-1091: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    91: Finding of No Significant Impact EA-1091: Finding of No Significant Impact Calderon Cokemaking Process/Demonstration Project The proposed Federal action, to provide cost-shared financial assistance for a demonstration project including the modification of an existing process demonstration unit and operation for producing metallurgical grade coke, does not constitute a major Federal action that would significantly affect the quality of the human environment as defined by NEPA. This conclusion

  8. EA-1236: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    Finding of No Significant Impact EA-1236: Finding of No Significant Impact Preparation for Transfer of Ownership of Naval Petroleum Reserve No. 3, Natrona County, Wyoming Based on the analysis of the Sitewide Environmental Assessment for Naval Petroleum Reserve No. 3 (NPR-3) DOE has determined that the proposed action to conduct activities in anticipation of possible transfer of NPR-3 out of Federal operation is not a major Federal action significantly affecting the quality of the human

  9. EA-1301: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    Finding of No Significant Impact EA-1301: Finding of No Significant Impact Captive Rearing Initiative for Salmon River Chinook Salmon Bonneville Power Administration (BPA), Department of Energy (DOE), is proposing to fund the Idaho Department of Fish and Game (IDFG) Captive Rearing Initiative for Salmon River Chinook Salmon Program (IDFG Program). The IDFG Program is a small-scale research and production initiative designed to increase numbers of three weak but recoverable populations of

  10. EA-1331: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    1: Finding of No Significant Impact EA-1331: Finding of No Significant Impact Remediation of Subsurface and Groundwater Contamination at the Rock Springs in situ Oil Shale Retort Site Based on the analysis and information provided in the EA, DOE has determined that the proposed Federal action, to conduct air sparging at the Rock Springs in situ Oil Shale Retort Site in Sweetwater County, Wyoming, does not constitute a major Federal action significantly affecting the quality of the human

  11. EA-1384: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    4: Finding of No Significant Impact EA-1384: Finding of No Significant Impact Proposed Improvements at the Thomas Jefferson National Accelerator Facility, Newport News, Virginia DOE evaluated the potential environmental impacts from proposed construction of various site improvements and the proposed installation and operation of the Helios light source at Jefferson Lab. The Southeastern Universities Research Association, Inc. operates Jefferson Lab (SURA) under contract to the DOE. With this

  12. EA-1488: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    8: Finding of No Significant Impact EA-1488: Finding of No Significant Impact U-233 Disposition, Medical Isotope Production, and Building 3019 Complex Shutdown at the Oak Ridge National Laboratory, Oak Ridge, Tennessee The U. S. Department of Energy has completed an Environmental Assessment (EA) [DOE/EA-1488] that evaluates the processing of uranium-233 (233U) stored at the Oak Ridge National Laboratory (ORNL) and other small quantities of similar material currently stored at other DOE sites in

  13. EA-1533: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    3: Finding of No Significant Impact EA-1533: Finding of No Significant Impact Proposed Gas Main and Distribution System Upgrade, Pantex Plant, Amarillo, Texas This environmental assessment (EA) provides sufficient evidence and analysis for the Department of Energy (DOE)/National Nuclear Security Administration (NNSA) to conclude that potential adverse effects from the upgrade of the distribution system within plant boundaries and the construction of a 10-inch gas main off the plant site for a

  14. EA-1534: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    Finding of No Significant Impact EA-1534: Finding of No Significant Impact Proposed Upgrade and Operation of the CEBAF and FEL Accelerators and Construction and Use of Buildings Associated with the 2005 Ten-Year Site Plan at the Thomas Jefferson National Accelerator Facility Newport News, Virginia DOE evaluated the potential environmental impacts from proposed construction of various site improvements and the proposed upgrade and operation of the CEBAF and FEL accelerators at Jefferson Lab. PDF

  15. EA-1535: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    5: Finding of No Significant Impact EA-1535: Finding of No Significant Impact Uranium Leasing Program U.S. Department of Energy Office of Legacy Management prepared the Uranium Leasing Program Programmatic Environmental Assessment (PEA) (DOE/EA-1535) to evaluate its management alternatives for the future of DOE's Uranium Leasing Program (ULP). Based on the analyses in the final PEA, I have determined that the proposed action does not constitute a major Federal action significantly affecting the

  16. EA-1562: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    Finding of No Significant Impact EA-1562: Finding of No Significant Impact Construction and Operation of a Physical Sciences Facility at the Pacific Northwest National Laboratory, Richland, Washington Based on the analyses of the environmental impacts in the Fina EA and consideration of public comments received on the draft EA, DOE has determined that the proposed action is not a major federal action significantly affecting the quality of the human environment within the meaning of NEPA.

  17. EA-1570: Final Environmental Assessment and Finding of No Significant

    Office of Environmental Management (EM)

    Impact | Department of Energy Assessment and Finding of No Significant Impact EA-1570: Final Environmental Assessment and Finding of No Significant Impact Construction and Operation of Neutrinos at the Main Injector Off-Axis Electron Neutrino Appearance Experiment (NOvA) at the Fermi National Accelerator Laboratory, Batavia, Illinois, and St. Louis County, Minnesota This Environmental Assessment (DOE/EA-1570) provides information and analysis of proposed U.S. Department of Energy activities

  18. EA-1602: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    2: Finding of No Significant Impact EA-1602: Finding of No Significant Impact Alternative Intake Project Transmission Line and Interconnection, California This notice provides Western Area Power Administration's National Environmental Poilcy Act determination regarding Western's proposal to provide an electric transmission line and interconnection to Contra Costa Water District's Alternative Intake Project (AIP) located in the Sacramento-San Joaquin Delta, within San Joaquin and Contra Costa

  19. EA-1605: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    Finding of No Significant Impact EA-1605: Finding of No Significant Impact Biomass Cogeneration and Heating Facilities at the Savannah River Site, South Carolina The Department of Energy has prepared an environmental assessment (EA) (DOE/EA-1605) to analyze the potential environmental impacts of the proposed construction and operation of new biomass cogeneration and heating facilities located at the Savannah River Site (SRS). DOE has determined that the proposed action is not a major Federal

  20. EA-1606: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    Finding of No Significant Impact EA-1606: Finding of No Significant Impact Proposed Use of Savannah River Site Lands for Military Training, SC DOE prepared an environmental assessment to analyze the potential environmental impacts of the proposed use of Savannah River Site lands and facilities for military training. Based on the analyses in the EA, DOE has determined that the proposed action is not a major Federal action significantly affecting the quality of the human environment within the

  1. EA-1631: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    1: Finding of No Significant Impact EA-1631: Finding of No Significant Impact Loan Guarantee for Beacon Power Corporation Frequency Regulation Facility in Stephentown, N.Y. The environmental assessment examines the potential environmental impacts associated with issuing a Federal loan guarantee to Beacon Power Corporation for construction and operation of a flywheel-based frequency regulation facility at an undeveloped seven acre site in Stephentown, New York. PDF icon Environmental Assessment

  2. EA-1640: Final Environmental Assessment and Finding of No Significant

    Office of Environmental Management (EM)

    Impact | Department of Energy 40: Final Environmental Assessment and Finding of No Significant Impact EA-1640: Final Environmental Assessment and Finding of No Significant Impact Transfer of Land and Facilities within the East Tennessee Technology Park and Surrounding Area, Oak Ridge, Tennessee DOE proposes to convey up to approximately 1,800 acres of property located within and surrounding the ETTP. This property includes the majority of the main ETTP plant area, Duct Island, a portion of

  3. EA-1651: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    1: Finding of No Significant Impact EA-1651: Finding of No Significant Impact U-233 Material Downblending and Disposition Project at the Oak Ridge National Laboratory, Oak Ridge, Tennessee This environmental assessment (EA) evaluates the impacts of planned activities to modify selected Oak Ridge National Laboratory (ORNL) facilities; process the ORNL inventory of uranium-233 (U-233); and transport the processed material to a long-term disposal facility. Based on the results of the analyses

  4. EA-1655: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    5: Finding of No Significant Impact EA-1655: Finding of No Significant Impact The Berkeley Lab Laser Accelerator (BELLA) Laser Acquisition, Installation and Use for Research and Development The Proposed Action would create and operate an experimental facility for further advancing the development of laser-driven, plasma-based, particle beam accelerators. An existing, approximately 7,000-square-foot, accelerator laboratory area inside Building 71 at Lawrence Berkeley National Laboratory (LBNL)

  5. EA-1699: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    99: Finding of No Significant Impact EA-1699: Finding of No Significant Impact Pope/Douglas Third Combustor Expansion Project, Alexandria, Minnesota The United States Department of Energy, National Energy Technology Laboratory (DOE NETL) prepared this Environmental Assessment (EA) to analyze the potential environmental impacts of providing funding for the proposed Pope/Douglas Third Combustor Expansion Project in Alexandria, Minnesota. Based on the EA, DOE determined that its proposed action

  6. EA-1726: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    Finding of No Significant Impact EA-1726: Finding of No Significant Impact Kahuku Wind Power, LLC, Construction of the Kahuku Wind Power Facility in Kahuku, O'ahu, Hawaii The Department of Energy conducted an environmental assessment that analyzed the potential environmental impacts associated with the proposed construction and operation of a 30 megawatt wind power facility on approximately 578 acres in the Kahuku area on the northeastern portion of the Island of O'ahu, within the State of

  7. EA-1815: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    5: Finding of No Significant Impact EA-1815: Finding of No Significant Impact Cuyahoga County Agricultural Society Wind Energy Project, Berea, Cuyahoga County, Ohio The Department of Energy's proposed action is to authorize the expenditure of Federal funding appropriated under the American Recovery and Reinvestment Act to design, permit, and construct the Cuyahoga County Agricultural Society Wind Energy Project, a 660 kilowatt wind turbine at Cuyahoga County Fairgrounds in the center of the

  8. EA-1828: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    28: Finding of No Significant Impact EA-1828: Finding of No Significant Impact Carbon Dioxide Capture from Biofuels Production and Sequestration into the Mount Simon Sandstone Phase II Project in Decatur, Illinois Based on the analyses in the environmental assessment DOE determined that its proposed action-awarding a grant to Archer Daniels Midland Company (ADM) to demonstrate an integrated system of carbon dioxide capture in an industrial setting and geologic sequestration in a sandstone

  9. EA-1839: Final Environmental Assessment and Finding of No Significant

    Office of Environmental Management (EM)

    Impact | Department of Energy 39: Final Environmental Assessment and Finding of No Significant Impact EA-1839: Final Environmental Assessment and Finding of No Significant Impact Department of Energy Loan Guarantee to Cogentrix of Alamosa, LLC for Construction of the Cogentrix Solar Project Near Alamosa, Colorado For more information, contact: Ms. Sharon Thomas NEPA Document Manager U.S. Department of Energy 1000 Independence Avenue, SW, LP-10 Washington, DC 20585 Electronic mail:

  10. EA-1840: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    Finding of No Significant Impact EA-1840: Finding of No Significant Impact Loan Guarantee to High Plains II, LLC for the California Valley Solar Ranch Project in San Luis Obispo County and Kern County, California California Valley Solar Ranch Project in San Luis Obispo and Kern Counties, California PDF icon EA-1840-FONSI-2011.pdf More Documents & Publications EA-1840: Final Environmental Assessment California Valley Solar Ranch Biological Assessment PG&E Reconductoring Project Biological

  11. EA-1849: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    Finding of No Significant Impact EA-1849: Finding of No Significant Impact Ormat Nevada Northern Nevada Geothermal Power Plant Projects: Loan Guarantee for ORMAT LLC's Tuscarora Geothermal Power Plant, Elko County, Nevada; Jersey Valley Geothermal Project, Pershing County, Nevada; and McGinness Hills Geothermal Project, Lander County, Nevada The U.S. Department of Energy has conducted an environmental assessment (EA) that analyzed the potential environmental impacts associated with issuing a

  12. EA-1891: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    Finding of No Significant Impact EA-1891: Finding of No Significant Impact Alvey-Fairview Transmission Line Rebuild Project, Oregon Based on the information in the EA, BPA determined that the proposed action is not a major federal action significantly affecting the quality of the human environment within the meaning of NEPA. Therefore, an EIS will not be prepared and BPA is issuing this FONSI for the proposed action. PDF icon EA-1891-FONSI-2014.pdf More Documents & Publications EA-1891:

  13. EA-1901: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    Finding of No Significant Impact EA-1901: Finding of No Significant Impact Kootenai River White Sturgeon and Burbot Hatcheries Project, Bonners Ferry, Boundary County, Idaho Based on the information in the EA, BPA has determined that the proposed action is not a major federal action that significantly affects the quality of the human environment, within the meaning of NEPA. Therefore, preparation of an EIS is not required, and BPA is issuing this FONSI. PDF icon EA-1901-FONSI-2013.pdf More

  14. EA-1918: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    8: Finding of No Significant Impact EA-1918: Finding of No Significant Impact Energy Efficiency Design Standards for New Federal Commercial and Multi-Family High-Rise Residential Buildings (Final Rule) Based upon the EA, DOE has determined that revising the Federal building energy efficiency standards for commercial and multi-family high-rise residential buildings to ASHRAE 90.1-2010 would not be a major Federal action significantly affecting the quality of the human environment within the

  15. EA-1929: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    29: Finding of No Significant Impact EA-1929: Finding of No Significant Impact NorthStar Medical Technologies LLC, Commercial Domestic Production of the Medical Isotope Molybdenum-99 NNSA has completed the Final Environmental Assessment for the NorthStar Medical Technologies LLC, Commercial Domestic Production of the Medical Isotope Molybdenum-99. Based upon the analysis in the EA, NNSA has determined that its proposed action-to provide financial assistance to Northstar Medical Technologies LLC

  16. EA-1934: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    Finding of No Significant Impact EA-1934: Finding of No Significant Impact Expansion of Active Borrow Areas, Hanford Site, Richland, Washington Based on the information presented in the Final EA and the commitment in the MAP to mitigate impacts to less-than-significant levels, DOE has determined that the proposed action to expand eleven active borrow areas on the Hanford Site will not constitute a major Federal action significantly affecting the quality of the human environment within the

  17. EA-1942: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    2: Finding of No Significant Impact EA-1942: Finding of No Significant Impact Cove Point Liquefaction Project, Lusby, Maryland The Federal Energy Regulatory Commission (FERC) issued an EA that examined the potential environmental impacts of a proposal to add natural gas liquefaction and exportation capabilities to the existing Cove Point LNG Terminal. DOE, Office of Fossil Energy, was a cooperating agency because it had an obligation under Section 3 of the Natural Gas Act to authorize the import

  18. Curved track segment finding using Tiny Triplet Finder (TTF)

    SciTech Connect (OSTI)

    Wu, Jin-Yuan; Wang, M.; Gottschalk, E.; Shi, Z.; /Fermilab

    2006-11-01

    We describe the applications of a track segment recognition scheme called the Tiny Triplet Finder (TTF) that involves the grouping of three hits satisfying a constraint forming of a track segment. The TTF was originally developed solving straight track segment finding problem, however, it is also suitable in many curved track segment finding problems. The examples discussed in this document are among popular detector layouts in high-energy/nuclear physics experiments. Although it is not practical to find a universal recipe for arbitrary detector layouts, the method of the TTF application is illustrated via the discussion of the examples. Generally speaking, whenever the data item to be found in a pattern recognition problem contains two free parameters, and if the constraint connecting the measurements and the two free parameters has an approximate shift invariant property, the Tiny Triplet Finder can be used.

  19. EA-1775: Finding of No Significant Impact | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1775: Finding of No Significant Impact Texas A&M University Combined Heat and Power Project, College Station, Texas DOE completed the Final Environmental Assessment for the Texas A&M University Combined Heat and power Project, College Station, Texas (DOE/EA-1775). Based on the analyses in the EA, DOE determined that its proposed action-awarding a federal grant to Texas A&A University to facilitate installatino and operation of a high-efficiency

  20. Research Finds Vitamin D Deficiency Affects Bone Quality

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Research Finds Vitamin D Deficiency Affects Bone Quality Research Finds Vitamin D Deficiency Affects Bone Quality Print Wednesday, 30 April 2014 00:00 Vitamin D deficiency is a widespread medical condition that plays a major role in human bone health. Scientists know that a lack of vitamin D can cause bone diseases such as rickets and osteomalacia. Now a team of researchers working at the ALS has also found that vitamin D deficiency plays a significant role in the bone-aging process. Low levels

  1. NREL Study Finds Barotrauma Not Guilty | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Study Finds Barotrauma Not Guilty NREL Study Finds Barotrauma Not Guilty January 10, 2013 - 3:18pm Addthis This is an excerpt from the Fourth Quarter 2012 edition of the Wind Program R&D Newsletter. For years scientists have hypothesized that the increasing number of bat fatalities in wind farms is due to barotrauma-related injuries. But according to a study presented by the National Renewable Energy Laboratory (NREL) at the ninth biennial Wind Wildlife Research Meeting in Denver, Colorado,

  2. Finding & Sharing Information about Energy Efficiency | Department of

    Office of Environmental Management (EM)

    Energy Finding & Sharing Information about Energy Efficiency Finding & Sharing Information about Energy Efficiency June 29, 2011 - 12:27pm Addthis Drew Bittner Writer/Editor, Office of Energy Efficiency and Renewable Energy Vacation time should be relaxing, but it's often just a different kind of work. Doesn't mean it isn't fun but I seem to come home from vacation more tired than when I left. This past week was spent on the Gulf Coast, where my mother turned 81. One of my goals was

  3. Malaria Researchers Find Weakness in Global Killer | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Malaria Researchers Find Weakness in Global Killer Malaria Researchers Find Weakness in Global Killer February 1, 2012 - 9:16am Addthis The protozoan <i>Plasmodium falciparum</i> gliding through a cell in the gut of a mosquito, its primary host. Although five different species of <i>Plasmodium</i> can cause malaria, <i>Plasmodium falciparum</i> causes the most severe disease. | Photo courtesy of Wikipedia Commons. The protozoan Plasmodium falciparum gliding

  4. EA-1528: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    Finding of No Significant Impact EA-1528: Finding of No Significant Impact Storage of Tritium-Producing Burnable Absorber Rods in K-Area Transfer Bay at the Savannah River Site The proposed action is to implement the storage of TPBARs in the K-Area dry storage Transfer Bay for a period of up to two years. The Transfer Bay is proposed for the storage of TPBARs in a 10 CFR 71-certified shipping package that offers a high degree of protection for the tritiated rods. The shipping cask, its outer ISO

  5. EA-1871: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    Finding of No Significant Impact EA-1871: Finding of No Significant Impact Energy Efficiency Design Standards for New Federal Commercial and Multi-Family High-Rise Residential Buildings and New Federal Low-Rise Residential Buildings Based on an DOE/EA-1871, DOE has determined that revising the Federal building energy efficiency standards to ASHRAE 90.1-2007 and IECC 2009 would not be a major Federal action significantly affecting the quality of the human environment within the meaning of NEPA.

  6. EA-1956: Finding of No Significant Impact | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    DOE issued a finding of no significant impacts for the proposed discontinuation of DOE operations at the Rocky Mountain Oilfield Testing Center (RMOTC) and the proposed divestiture of Naval Petroleum Reserve Number 3 (NPR-3). PDF icon EA-1956-FONSI-2014.pdf More Documents & Publications EA-1956: Final Environmental Assessment EA-1956: Draft Site-Wide Environmental Assessment EA-1236

  7. Finding the needle in the haystack: Algorithms for conformational optimization

    SciTech Connect (OSTI)

    Andricioaei, I.; Straub, J.E.

    1996-09-01

    Algorithms are given for comformational optimization of proteins. The protein folding problems is regarded as a problem of global energy mimimization. Since proteins have hundreds of atoms, finding the lowest-energy conformation in a many-dimensional configuration space becomes a computationally demanding problem.{copyright} {ital American Institute of Physics.}

  8. Livermore research finds that climate models overestimate rainfall

    National Nuclear Security Administration (NNSA)

    increases | National Nuclear Security Administration research finds that climate models overestimate rainfall increases | National Nuclear Security Administration Facebook Twitter Youtube Flickr RSS People Mission Managing the Stockpile Preventing Proliferation Powering the Nuclear Navy Emergency Response Recapitalizing Our Infrastructure Countering Nuclear Terrorism About Our Programs Our History Who We Are Our Leadership Our Locations Budget Our Operations Library Bios Congressional

  9. NREL analysis finds tax credit extensions can impact renewable energy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    deployment and electric sector CO2 emissions - News Releases | NREL analysis finds tax credit extensions can impact renewable energy deployment and electric sector CO2 emissions February 22, 2016 The Energy Department's National Renewable Energy Laboratory (NREL) today released new analysis exploring the potential impact of recently extended federal tax credits on the deployment of renewable generation technologies and related U.S. electric sector carbon dioxide (CO2) emissions. The report,

  10. Summer interns present research findings in poster session | Princeton

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Plasma Physics Lab Summer interns present research findings in poster session By Raphael Rosen August 31, 2015 Tweet Widget Google Plus One Share on Facebook New Jersey Assemblywoman Donna Simon Talks With SULI Intern Cara Bagley (Photo by Elle Starkman) New Jersey Assemblywoman Donna Simon Talks With SULI Intern Cara Bagley Gallery: Physicist Charles Skinner and Intern Amanda Lewis (Photo by Elle Starkman) Physicist Charles Skinner and Intern Amanda Lewis Interns Matthew Lotocki, Zack

  11. EA-1796: Finding of No Significant Impact | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    Sempra Generation for Construction of the Mesquite Solar Energy Project; Maricopa County, Arizona Summary Summary DOE's Western Area Power Administration issued a FONSI for the power purchase agreement, on behalf of the Navy, for renewable energy that would be generated by the Mesquite Solar Energy Project. Documents Available For Download PDF icon EA-1796 FONSI 2015 More Documents & Publications EA-1796: Final Environmental Assessment EA-1796: Finding of No Significant Impact MESQUITE

  12. Perpendicular Magnetism Unparalleled Find in Single Crystal | The Ames

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Laboratory Perpendicular Magnetism Unparalleled Find in Single Crystal Two distinct types of magnetism aligned perpendicular in a single crystal have been detailed in new measurements on single-crystal and powered samples composed of barium, potassium, manganese, and arsenic. Antiferromagnetism occurs with a checkerboard-style patterning of the total atomic magnetic moments due to the spins of the localized electrons of the manganese atoms (known as 'local-moment magnetism'). Aligning

  13. Fermilab | Science | Particle Physics 101 | How to find the smallest

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    particles - Particle Detectors How to find the smallest particles - Particle Detectors Scientists have invented and built many types of devices to detect particles. Most experiments at Fermilab use huge detectors that consist of intricate configurations of different detection devices, designed to collect the most possible information about the different types of particles created in a particle collision. Different types of particle detectors: Scintillators - When a high energy particle

  14. NREL Scientists Find Key Function for Ferredoxins in Algae Hydrogen

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Production - News Releases | NREL Scientists Find Key Function for Ferredoxins in Algae Hydrogen Production Two of six iron-rich proteins shown to have role in algae metabolism; discovery could lead to enhanced hydrogen production February 10, 2014 Scientists at the Energy Department's National Renewable Energy Laboratory have demonstrated that just two of six iron-sulfur-containing ferredoxins in a representative species of algae promote electron transfers to and from hydrogenases. The

  15. DOE issues Finding of No Significant Impact for the Environmental

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Assessment on Resumption of Transient Testing of Nuclear Fuels and Materials at Idaho National Laboratory Tim Jackson, (208) 526-8484 For Immediate Release: February 28, 2014 DOE issues Finding of No Significant Impact for the Environmental Assessment on Resumption of Transient Testing of Nuclear Fuels and Materials at Idaho National Laboratory The U.S. Department of Energy (DOE) has determined that resuming transient testing of nuclear fuels and materials at Idaho National Laboratory (INL)

  16. FINDING OF NO SIGNIFICANT IMPACT WESTERN AREA POWER ADMINISTRATION

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    OF NO SIGNIFICANT IMPACT WESTERN AREA POWER ADMINISTRATION POWER PURCHASE AGREEMENT FOR PART 2 OF THE MESQUITE SOLAR ENERGY PROJECT IN MARICOPA COUNTY, ARIZONA AGENCY: Western Area Power Administration ACTION: Finding of No Significant Impact SUMMARY: In 2010 and 2011, the U.S. Department of Energy (DOE) conducted an environmental assessment (EA) (DOE/EA-1796) to analyze the potential environmental impacts associated with issuing a Federal loan guarantee to Sempra Generation (Sempra) for

  17. FOR THE SUPPLEMENTAL ENVIRONMENTAL ASSESSMENT/FINDING OF NO

    Office of Environmental Management (EM)

    2 FOR THE SUPPLEMENTAL ENVIRONMENTAL ASSESSMENT/FINDING OF NO SIGNIFICANT IMPACT FOR THE UNIVERSITY OF MAINE'S DEEPWATER OFFSHORE FLOATING WIND TURBINE TESTING AND DEMONSTRATION PROJECT CASTINE, MAINE DOE/EA-1792-S1 US Department of Energy Office of Energy Efficiency and Renewable Energy Golden, Colorado November 2014 Supplement Analysis - 2 i November 20, 2014 DOE/EA-1792-S1 CONTENTS 1.0 Background

  18. Finding Solutions to Solar's Soft Cost Dilemma | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Solutions to Solar's Soft Cost Dilemma Finding Solutions to Solar's Soft Cost Dilemma January 8, 2013 - 1:22pm Addthis Brian Webster installs rooftop solar panels on a home in Englewood, Colorado. The Energy Department is working to streamline rooftop solar installations so that its faster, easier and cheaper for Americans to go solar. | Photo courtesy of Dennis Schroeder, NREL. Brian Webster installs rooftop solar panels on a home in Englewood, Colorado. The Energy Department is working to

  19. EA-1732: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    Next Autoworks Louisiana, LLC (Formerly V-Vehicle Company), Advanced Technology Gasoline Vehicle Manufacturing Project in Monroe, Louisiana The Department of Energy has conducted an environmental assessment that analyzed the potential environmental impacts associated with the production of an advanced technology gasoline-powered four-door hatchback vehicle named the V Car. PDF icon Finding of No Significant Impact Department of Energy Loan to Next Autoworks Louisiana, LLC (Formerly V-Vehicle

  20. EA-1732: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    Department of Energy Loan to Next Autoworks Louisiana, LLC (Formerly V-Vehicle Company) for an Advanced Technology Gasoline Vehicle Manufacturing Project in Monroe, Louisiana Department of Energy proposes award a federal grant to Johnson Controls, Inc., and ENTEK International, LLC to establish an advanced lithium ion battery manufacturing facility. PDF icon Finding of No Significant Impact for Johnson Controls, Inc., and Entek International, LLC Electric Drive Vehicle Battery and Component

  1. Molecular genetic analysis of activation-tagged transcription factors thought to be involved in photomorphogenesis

    SciTech Connect (OSTI)

    Neff, Michael M.

    2011-06-23

    This is a final report for Department of Energy Grant No. DE-FG02-08ER15927 entitled Molecular Genetic Analysis of Activation-Tagged Transcription Factors Thought to be Involved in Photomorphogenesis. Based on our preliminary photobiological and genetic analysis of the sob1-D mutant, we hypothesized that OBP3 is a transcription factor involved in both phytochrome and cryptochrome-mediated signal transduction. In addition, we hypothesized that OBP3 is involved in auxin signaling and root development. Based on our preliminary photobiological and genetic analysis of the sob2-D mutant, we also hypothesized that a related gene, LEP, is involved in hormone signaling and seedling development.

  2. Role of a Transcriptional Regulator in Programmed Cell Death and Plant Development

    SciTech Connect (OSTI)

    Julie M. Stone

    2008-09-13

    The long-term goal of this research is to understand the role(s) and molecular mechanisms of programmed cell death (PCD) in the controlling plant growth, development and responses to biotic and abiotic stress. We developed a genetic selection scheme to identify A. thaliana FB1-resistant (fbr) mutants as a way to find genes involved in PCD (Stone et al., 2000; Stone et al., 2005; Khan and Stone, 2008). The disrupted gene in fbr6 (AtSPL14) responsible for the FB1-insensitivity and plant architecture phenotypes encodes a plant-specific SBP DNA-binding domain transcriptional regulator (Stone et al., 2005; Liang et al., 2008). This research plan is designed to fill gaps in the knowledge about the role of SPL14 in plant growth and development. The work is being guided by three objectives aimed at determining the pathways in which SPL14 functions to modulate PCD and/or plant development: (1) determine how SPL14 functions in plant development, (2) identify target genes that are directly regulated by SPL14, and (3) identify SPL14 modifications and interacting proteins. We made significant progress during the funding period. Briefly, some major accomplishments are highlighted below: (1) To identify potential AtSPL14 target genes, we identified a consensus DNA binding site for the AtSPL14 SBP DNA-binding domain using systematic evolution of ligands by exponential selection (SELEX) and site-directed mutagenesis (Liang et al., 2008). This consensus binding site was used to analyze Affymetrix microarray gene expression data obtained from wild-type and fbr6 mutant plants to find possible AtSPL14-regulated genes. These candidate AtSPL14-regulated genes are providing new information on the molecular mechanisms linking plant PCD and plant development through modulation of the 26S proteasome. (2) Transgenic plants expressing epitope-tagged versions of AtSPL14 are being used to confirm the AtSPL14 targets (by ChIP-PCR) and further dissect the molecular interactions (Nazarenus, Liang and Stone, in preparation) (3) Double mutants generated between fbr6 and various accelerated cell death (acd) mutants indicate that sphingolipid metabolism is influenced by AtSPL14 and sphingolipidomics profiling supports this conclusion (Lin, Markham and Stone, in preparation). (4) A new set of phenotypes have been uncovered in the original fbr6-1 mutant, including a short-root phenotype related to auxin signaling and altered photosynthetic parameters related to stomatal density and conductance (Lin and Stone, in preparation; Lin, Madhavan and Stone, in preparation). Additional AtSPL14-related mutants and transgenic plants have been generated to effectively dissect the functions of AtSPL14, including a dominant negative fbr6-2 allele and transgenic plants overexpressing FBR6/AtSPL14 that display an accelerated cell death (acd) phenotype.

  3. EA-2002: Finding of No Significant Impact | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    Right-of-Way Application for the Tucson-Apache 115-kV Transmission Line, Pima County, Arizona Western Area Power Administration issued a Finding of No Significant Impact for obtaining, from the Bureau of Indian Affairs, a 150-foot right-of-way across tribal land along the existing Tucson-Apache Transmission Line to replace the previous 100-foot right-of-way, which has expired. PDF icon EA-2002-FONSI-2015.pdf More Documents & Publications EA-2002: Final Environmental Assessment CX-007172:

  4. Scientist finds new way to predict heat layer troublemaker | Princeton

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Plasma Physics Lab Scientist finds new way to predict heat layer troublemaker By John Greenwald August 27, 2012 Tweet Widget Google Plus One Share on Facebook Researchers at a recent worldwide conference on fusion power have confirmed the surprising accuracy of a new model for predicting the size of a key barrier to fusion that a top scientist at the U.S. Department of Energy's Princeton Plasma Physics Laboratory (PPPL) has developed. The model could serve as a starting point for overcoming

  5. Graph Coarsening for Path Finding in Cybersecurity Graphs

    SciTech Connect (OSTI)

    Hogan, Emilie A.; Johnson, John R.; Halappanavar, Mahantesh

    2013-01-01

    n the pass-the-hash attack, hackers repeatedly steal password hashes and move through a computer network with the goal of reaching a computer with high level administrative privileges. In this paper we apply graph coarsening in network graphs for the purpose of detecting hackers using this attack or assessing the risk level of the network's current state. We repeatedly take graph minors, which preserve the existence of paths in the graph, and take powers of the adjacency matrix to count the paths. This allows us to detect the existence of paths as well as find paths that have high risk of being used by adversaries.

  6. Transportation Energy Futures: Project Overview and Findings (Presentation)

    SciTech Connect (OSTI)

    Not Available

    2013-03-01

    The U.S. Department of Energy-sponsored Transportation Energy Futures (TEF) project examines how combining multiple strategies could reduce both GHG emissions and petroleum use by 80%. The project's primary objective was to help inform domestic decisions about transportation energy strategies, priorities, and investments, with an emphasis on previously underexplored opportunities related to energy efficiency and renewable energy in light-duty vehicles, non-light-duty vehicles, fuels, and transportation demand. This PowerPoint provides an overview of the project and its findings.

  7. Research Finds Vitamin D Deficiency Affects Bone Quality

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Research Finds Vitamin D Deficiency Affects Bone Quality Print Vitamin D deficiency is a widespread medical condition that plays a major role in human bone health. Scientists know that a lack of vitamin D can cause bone diseases such as rickets and osteomalacia. Now a team of researchers working at the ALS has also found that vitamin D deficiency plays a significant role in the bone-aging process. Low levels of vitamin D, the "sunshine vitamin," have been previously linked to the

  8. Research Finds Vitamin D Deficiency Affects Bone Quality

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Research Finds Vitamin D Deficiency Affects Bone Quality Print Vitamin D deficiency is a widespread medical condition that plays a major role in human bone health. Scientists know that a lack of vitamin D can cause bone diseases such as rickets and osteomalacia. Now a team of researchers working at the ALS has also found that vitamin D deficiency plays a significant role in the bone-aging process. Low levels of vitamin D, the "sunshine vitamin," have been previously linked to the

  9. Research Finds Vitamin D Deficiency Affects Bone Quality

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Research Finds Vitamin D Deficiency Affects Bone Quality Print Vitamin D deficiency is a widespread medical condition that plays a major role in human bone health. Scientists know that a lack of vitamin D can cause bone diseases such as rickets and osteomalacia. Now a team of researchers working at the ALS has also found that vitamin D deficiency plays a significant role in the bone-aging process. Low levels of vitamin D, the "sunshine vitamin," have been previously linked to the

  10. Research Finds Vitamin D Deficiency Affects Bone Quality

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Research Finds Vitamin D Deficiency Affects Bone Quality Print Vitamin D deficiency is a widespread medical condition that plays a major role in human bone health. Scientists know that a lack of vitamin D can cause bone diseases such as rickets and osteomalacia. Now a team of researchers working at the ALS has also found that vitamin D deficiency plays a significant role in the bone-aging process. Low levels of vitamin D, the "sunshine vitamin," have been previously linked to the

  11. ORISE: Study finds foreign doctorate recipients' stay rates remain high

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Study finds rate of foreign doctorate recipients staying in the United States remains high No evidence that visa restrictions are reducing stay rates, according to report FOR IMMEDIATE RELEASE Jan. 18, 2012 FY12-12 OAK RIDGE, Tenn.-The number of foreign students pursuing science and engineering doctorates in the United States continues to trend upward, and the rates at which they remain in the United States to work after graduation are at or near the highest levels observed for the various

  12. Research Finds Vitamin D Deficiency Affects Bone Quality

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Research Finds Vitamin D Deficiency Affects Bone Quality Print Vitamin D deficiency is a widespread medical condition that plays a major role in human bone health. Scientists know that a lack of vitamin D can cause bone diseases such as rickets and osteomalacia. Now a team of researchers working at the ALS has also found that vitamin D deficiency plays a significant role in the bone-aging process. Low levels of vitamin D, the "sunshine vitamin," have been previously linked to the

  13. Research Finds Vitamin D Deficiency Affects Bone Quality

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Research Finds Vitamin D Deficiency Affects Bone Quality Print Vitamin D deficiency is a widespread medical condition that plays a major role in human bone health. Scientists know that a lack of vitamin D can cause bone diseases such as rickets and osteomalacia. Now a team of researchers working at the ALS has also found that vitamin D deficiency plays a significant role in the bone-aging process. Low levels of vitamin D, the "sunshine vitamin," have been previously linked to the

  14. Exploring Plasma Science Advances from Fusion Findings to Astrophysical

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Achievements | Princeton Plasma Physics Lab Exploring Plasma Science Advances from Fusion Findings to Astrophysical Achievements By John Greenwald December 4, 2012 Tweet Widget Google Plus One Share on Facebook The latest advances in plasma physics were the focus of more than 1,000 scientists from around the world who gathered in Providence, R.I., from Oct. 29 through Nov. 2 for the 54th Annual Meeting of the American Physical Society's Division of Plasma Physics (APS-DPP). Papers, posters

  15. Finding Large Aperture Fractures in Geothermal Resource Areas Using A

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Three-Component Long-Offset Surface Seismic Survey, PSInSAR, and Kinematic Analysis | Department of Energy A Three-Component Long-Offset Surface Seismic Survey, PSInSAR, and Kinematic Analysis Finding Large Aperture Fractures in Geothermal Resource Areas Using A Three-Component Long-Offset Surface Seismic Survey, PSInSAR, and Kinematic Analysis Fining Large Aperture Fractures in Geothermal Resource Areas Using A Three-Component Long-Offset Surface Seismic Survey, PSInSAR, and Kinematic

  16. Microsoft Word - fact sheet alternatives and key findings 090214.docx

    National Nuclear Security Administration (NNSA)

    Alternatives and Key Findings of the Draft SEIS ALTERNATIVES The Draft SEIS evaluates a range of reactor site and tritium production capacity alternatives. Seven alternatives are analyzed in the Draft SEIS:  Alternative 1 Watts Bar site only (2,500 TPBARs maximum). This is the preferred alternative.  Alternative 2 Sequoyah site only (2,500 TPBARs maximum).  Alternative 3 Both Watts Bar and Sequoyah sites (2,500 TPBARs maximum).  Alternative 4 Watts Bar site only (5,000 TPBARs

  17. Chk2 regulates transcription-independent p53-mediated apoptosis in response to DNA damage

    SciTech Connect (OSTI)

    Chen Chen [Department of Geriatric Research, National Institute for Longevity Sciences, National Center for Geriatrics and Gerontology, Obu, Aichi 474-8522 (Japan); Shimizu, Shigeomi [Department of Post-Genomics Diseases, Osaka University Medical School, Suita, Osaka 565-0871 (Japan); Tsujimoto, Yoshihide [Department of Post-Genomics Diseases, Osaka University Medical School, Suita, Osaka 565-0871 (Japan); Motoyama, Noboru [Department of Geriatric Research, National Institute for Longevity Sciences, National Center for Geriatrics and Gerontology, Obu, Aichi 474-8522 (Japan)]. E-mail: motoyama@nils.go.jp

    2005-07-29

    The tumor suppressor protein p53 plays a central role in the induction of apoptosis in response to genotoxic stress. The protein kinase Chk2 is an important regulator of p53 function in mammalian cells exposed to ionizing radiation (IR). Cells derived from Chk2-deficient mice are resistant to the induction of apoptosis by IR, and this resistance has been thought to be a result of the defective transcriptional activation of p53 target genes. It was recently shown, however, that p53 itself and histone H1.2 translocate to mitochondria and thereby induces apoptosis in a transcription-independent manner in response to IR. We have now examined whether Chk2 also regulates the transcription-independent induction of apoptosis by p53 and histone H1.2. The reduced ability of IR to induce p53 stabilization in Chk2-deficient thymocytes was associated with a marked impairment of p53 and histone H1 translocation to mitochondria. These results suggest that Chk2 regulates the transcription-independent mechanism of p53-mediated apoptosis by inducing stabilization of p53 in response to IR.

  18. Finding Text Information in the Ocean of Electronic Documents

    SciTech Connect (OSTI)

    Medvick, Patricia A.; Calapristi, Augustin J.

    2003-02-05

    Information management in natural resources has become an overwhelming task. A massive amount of electronic documents and data is now available for creating informed decisions. The problem is finding the relevant information to support the decision-making process. Determining gaps in knowledge in order to propose new studies or to determine which proposals to fund for maximum potential is a time-consuming and difficult task. Additionally, available data stores are increasing in complexity; they now may include not only text and numerical data, but also images, sounds, and video recordings. Information visualization specialists at Pacific Northwest National Laboratory (PNNL) have software tools for exploring electronic data stores and for discovering and exploiting relationships within data sets. These provide capabilities for unstructured text explorations, the use of data signatures (a compact format for the essence of a set of scientific data) for visualization (Wong et al 2000), visualizations for multiple query results (Havre et al. 2001), and others (http://www.pnl.gov/infoviz ). We will focus on IN-SPIRE, a MS Windows vision of PNNLs SPIRE (Spatial Paradigm for Information Retrieval and Exploration). IN-SPIRE was developed to assist information analysts find and discover information in huge masses of text documents.

  19. Parallel halo finding in N-body cosmology simulations

    SciTech Connect (OSTI)

    Pfitzner, D.W.; Salmon, J.K.

    1996-12-31

    Cosmological N-body simulations on parallel computers produce large datasets - about five hundred Megabytes at a single output time, or tens of Gigabytes over the course of a simulation. These large datasets require further analysis before they can be compared to astronomical observations. We have implemented two methods for performing halo finding, a key part of the knowledge discovery process, on parallel machines. One of these is a parallel implementation of the friends of friends (FOF) algorithm, widely used in the field of N-body cosmology. The new isodensity (ID) method has been developed to overcome some of the shortcomings of FOR Both have been implemented on a variety of computer systems, and successfully used to extract halos from simulations with up to 256{sup 3} (or about 16.8 million) particles, which axe among the largest N-body cosmology simulations in existence.

  20. Utility of Social Modeling for Proliferation Assessment - Preliminary Findings

    SciTech Connect (OSTI)

    Coles, Garill A.; Gastelum, Zoe N.; Brothers, Alan J.; Thompson, Sandra E.

    2009-07-16

    Often the methodologies for assessing proliferation risk are focused around the inherent vulnerability of nuclear energy systems and associated safeguards. For example an accepted approach involves ways to measure the intrinsic and extrinsic barriers to potential proliferation. This paper describes preliminary investigation into non-traditional use of social and cultural information to improve proliferation assessment and advance the approach to assessing nuclear material diversion. Proliferation resistance assessment, safeguard assessments and related studies typically create technical information about the vulnerability of a nuclear energy system to diversion of nuclear material. The purpose of this research project is to find ways to integrate social information with technical information by explicitly considering the role of culture, groups and/or individuals to factors that impact the possibility of proliferation. When final, this work is expected to describe and demonstrate the utility of social science modeling in proliferation and proliferation risk assessments.

  1. Low concentrations of bisphenol a suppress thyroid hormone receptor transcription through a nongenomic mechanism

    SciTech Connect (OSTI)

    Sheng, Zhi-Guo; Tang, Yuan; Liu, Yu-Xiang; Yuan, Ye; Zhao, Bao-Quan; Chao, Xi-Juan; Zhu, Ben-Zhan

    2012-02-15

    Bisphenol (BPA) is one of the highest-volume chemicals produced worldwide, and human exposure to BPA is thought to be ubiquitous. Various rodent and in vitro studies have shown that thyroid hormone (TH) function can be impaired by BPA. However, it is still unknown if low concentrations of BPA can suppress the thyroid hormone receptor (TR) transcription. The present study aims to investigate the possible suppressing effects of low concentrations of BPA on TR transcription and the involved mechanism(s) in CV-1 cells derived from cercopithecus aethiops monkey kidneys. Using gene reporter assays, BPA at concentrations as low as 10{sup ?9} M suppresses TR or steroid receptor coactivator-1(SRC-1)-enhanced TR transcription, but not reducing TR/SRC-1 interaction in mammalian two-hybrid and glutathione S-transferase pull-down studies. It has been further shown that both nuclear receptor co-repressor (N-CoR) and silencing mediator for retinoid and thyroid hormone receptors (SMRT) are recruited to the TR-?1 by BPA in the presence of physiologic concentrations of T3 or T4. However, the overexpression of ?3 integrin or c-Src significantly reduces BPA-induced recruitment of N-CoR/SMRT to TR or suppression of TR transcription. Furthermore, BPA inhibits the T3/T4-mediated interassociation of the ?3 integrin/c-Src/MAPK/TR-?1 pathways by the co-immunoprecipitation. These results indicate that low concentrations of BPA suppress the TR transcription by disrupting physiologic concentrations of T3/T4-mediated ?3 integrin/c-Src/MAPK/TR-?1 pathways, followed by recruiting N-CoR/SMRT to TR-?1, providing a novel insight regarding the TH disruption effects of low concentration BPA. -- Highlights: ? Environmentally relevant concentrations of BPA suppress TR transcription. ? BPA recruits the N-CoR/SMRT to TR under the physiologic concentrations of T3/T4. ? BPA disrupts T3/T4-mediated ?3 integrin/c-Src/MAPK/TR-?1 pathways.

  2. CITED2 modulates estrogen receptor transcriptional activity in breast cancer cells

    SciTech Connect (OSTI)

    Lau, Wen Min; Doucet, Michele; Huang, David; Weber, Kristy L.; Kominsky, Scott L.

    2013-07-26

    Highlights: The effects of elevated CITED2 on ER function in breast cancer cells are examined. CITED2 enhances cell growth in the absence of estrogen and presence of tamoxifen. CITED2 functions as a transcriptional co-activator of ER in breast cancer cells. -- Abstract: Cbp/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain 2 (CITED2) is a member of the CITED family of non-DNA binding transcriptional co-activators of the p300/CBP-mediated transcription complex. Previously, we identified CITED2 as being overexpressed in human breast tumors relative to normal mammary epithelium. Upon further investigation within the estrogen receptor (ER)-positive subset of these breast tumor samples, we found that CITED2 mRNA expression was elevated in those associated with poor survival. In light of this observation, we investigated the effect of elevated CITED2 levels on ER function. While ectopic overexpression of CITED2 in three ER-positive breast cancer cell lines (MCF-7, T47D, and CAMA-1) did not alter cell proliferation in complete media, growth was markedly enhanced in the absence of exogenous estrogen. Correspondingly, cells overexpressing CITED2 demonstrated reduced sensitivity to the growth inhibitory effects of the selective estrogen receptor modulator, 4-hydroxytamoxifen. Subsequent studies revealed that basal ER transcriptional activity was elevated in CITED2-overexpressing cells and was further increased upon the addition of estrogen. Similarly, basal and estrogen-induced expression of the ER-regulated genes trefoil factor 1 (TFF1) and progesterone receptor (PGR) was higher in cells overexpressing CITED2. Concordant with this observation, ChIP analysis revealed higher basal levels of CITED2 localized to the TFF-1 and PGR promoters in cells with ectopic overexpression of CITED2, and these levels were elevated further in response to estrogen stimulation. Taken together, these data indicate that CITED2 functions as a transcriptional co-activator of ER in breast cancer cells and that its increased expression in tumors may result in estrogen-independent ER activation, thereby reducing estrogen dependence and response to anti-estrogen therapy.

  3. EA-1616-S1: Finding of No Significant Impact | Department of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finding of No Significant Impact EA-1616-S1: Finding of No Significant Impact National Carbon Capture Center Project at Southern Company Services' Power Systems Development...

  4. New Find-a-Car App Brings Fuel Economy Right to Your Phone | Department of

    Office of Environmental Management (EM)

    Energy Find-a-Car App Brings Fuel Economy Right to Your Phone New Find-a-Car App Brings Fuel Economy Right to Your Phone February 12, 2016 - 2:45pm Addthis New Find-a-Car App Brings Fuel Economy Right to Your Phone New Find-a-Car App Brings Fuel Economy Right to Your Phone New Find-a-Car App Brings Fuel Economy Right to Your Phone New Find-a-Car App Brings Fuel Economy Right to Your Phone New Find-a-Car App Brings Fuel Economy Right to Your Phone New Find-a-Car App Brings Fuel Economy Right

  5. The oncoprotein HBXIP upregulates PDGFB via activating transcription factor Sp1 to promote the proliferation of breast cancer cells

    SciTech Connect (OSTI)

    Zhang, Yingyi; Zhao, Yu; Li, Leilei; Shen, Yu; Cai, Xiaoli; Zhang, Xiaodong; Ye, Lihong

    2013-05-03

    Highlights: HBXIP is able to upregulate the expression of PDGFB in breast cancer cells. HBXIP serves as a coactivator of activating transcription factor Sp1. HBXIP stimulates the PDGFB promoter via activating transcription factor Sp1. HBXIP promotes the proliferation of breast cancer cell via upregulating PDGFB. -- Abstract: We have reported that the oncoprotein hepatitis B virus X-interacting protein (HBXIP) acts as a novel transcriptional coactivator to promote proliferation and migration of breast cancer cells. Previously, we showed that HBXIP was able to activate nuclear factor-?B (NF-?B) in breast cancer cells. As an oncogene, the platelet-derived growth factor beta polypeptide (PDGFB) plays crucial roles in carcinogenesis. In the present study, we found that both HBXIP and PDGFB were highly expressed in breast cancer cell lines. Interestingly, HBXIP was able to increase transcriptional activity of NF-?B through PDGFB, suggesting that HBXIP is associated with PDGFB in the cells. Moreover, HBXIP was able to upregulate PDGFB at the levels of mRNA, protein and promoter in the cells. Then, we identified that HBXIP stimulated the promoter of PDGFB through activating transcription factor Sp1. In function, HBXIP enhanced the proliferation of breast cancer cells through PDGFB in vitro. Thus, we conclude that HBXIP upregulates PDGFB via activating transcription factor Sp1 to promote proliferation of breast cancer cells.

  6. Inhibition of hepatitis B virus (HBV) by LNA-mediated nuclear interference with HBV DNA transcription

    SciTech Connect (OSTI)

    Sun, Zhen; Department of Biochemistry and Molecular Biology, Program in Molecular Cell Biology, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058 ; Xiang, Wenqing; Guo, Yajuan; Chen, Zhi; Liu, Wei; Lu, Daru

    2011-06-10

    Highlights: {yields} LNA-modified oligonucleotides can pass through the plasma membrane of cultured cells even without using transfection machinery. {yields} LNA-modified oligonucleotides passed efficiently across the cell membrane, and lipid-coating facilitated translocation from the cytoplasm to the nucleus. {yields} LNA-oligonucleotide designed to target nuclear HBV DNA efficiently suppresses HBV replication and transcription in cultured hepatic cells. -- Abstract: Silencing target genes with small regulatory RNAs is widely used to investigate gene function and therapeutic drug development. Recently, triplex-based approaches have provided another attractive means to achieve targeted gene regulation and gene manipulation at the molecular and cellular levels. Nuclear entry of oligonucleotides and enhancement of their affinity to the DNA targets are key points of such approaches. In this study, we developed lipid-based transport of a locked-nucleic-acid (LNA)-modified oligonucleotide for hepatitis B virus (HBV) DNA interference in human hepatocytes expressing HBV genomic DNA. In these cells, the LNA-modified oligonucleotides passed efficiently across the cell membrane, and lipid-coating facilitated translocation from the cytoplasm to the nucleus. The oligonucleotide specifically targeting HBV DNA clearly interfered with HBV DNA transcription as shown by a block in pregenomic RNA (pgRNA) production. The HBV DNA-targeted oligonucleotide suppressed HBV DNA replication and HBV protein production more efficiently than small interfering RNAs directed to the pgRNA. These results demonstrate that fusion with lipid can carry LNA-modified oligonucleotides to the nucleus where they regulate gene expression. Interfering with HBV DNA transcription by LNA-modified oligonucleotides has strong potential as a new strategy for HBV inhibition.

  7. Structurally distinct polycyclic aromatic hydrocarbons induce differential transcriptional responses in developing zebrafish

    SciTech Connect (OSTI)

    Goodale, Britton C.; Tilton, Susan C.; Corvi, Margaret M.; Wilson, Glenn R.; Janszen, Derek B.; Anderson, Kim A.; Waters, Katrina M.; Tanguay, Robert L.

    2013-11-01

    Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous in the environment as components of fossil fuels and by-products of combustion. These multi-ring chemicals differentially activate the aryl hydrocarbon receptor (AHR) in a structurally dependent manner, and induce toxicity via both AHR-dependent and -independent mechanisms. PAH exposure is known to induce developmental malformations in zebrafish embryos, and recent studies have shown cardiac toxicity induced by compounds with low AHR affinity. Unraveling the potentially diverse molecular mechanisms of PAH toxicity is essential for understanding the hazard posed by complex PAH mixtures present in the environment. We analyzed transcriptional responses to PAH exposure in zebrafish embryos exposed to benz(a)anthracene (BAA), dibenzothiophene (DBT) and pyrene (PYR) at concentrations that induced developmental malformations by 120 h post-fertilization (hpf). Whole genome microarray analysis of mRNA expression at 24 and 48 hpf identified genes that were differentially regulated over time and in response to the three PAH structures. PAH body burdens were analyzed at both time points using GCMS, and demonstrated differences in PAH uptake into the embryos. This was important for discerning dose-related differences from those that represented unique molecular mechanisms. While BAA misregulated the least number of transcripts, it caused strong induction of cyp1a and other genes known to be downstream of the AHR, which were not induced by the other two PAHs. Analysis of functional roles of misregulated genes and their predicted regulatory transcription factors also distinguished the BAA response from regulatory networks disrupted by DBT and PYR exposure. These results indicate that systems approaches can be used to classify the toxicity of PAHs based on the networks perturbed following exposure, and may provide a path for unraveling the toxicity of complex PAH mixtures. - Highlights: Defined global mRNA expression changes induced by developmental exposure to PAHs Determined PAH body burdens following developmental exposure Genes uniquely induced by benz(a)anthracene included targets of the AHR and RELA Dibenzothiophene and pyrene perturbed a distinct RELA network Transcriptional networks reveal differential mechanisms of PAH toxicity.

  8. ECRbase: Database of Evolutionary Conserved Regions, Promoters, and Transcription Factor Binding Sites in Vertebrate Genomes

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Loots, Gabriela G. [LLNL; Ovcharenko, I. [LLNL

    Evolutionary conservation of DNA sequences provides a tool for the identification of functional elements in genomes. This database of evolutionary conserved regions (ECRs) in vertebrate genomes features a database of syntenic blocks that recapitulate the evolution of rearrangements in vertebrates and a comprehensive collection of promoters in all vertebrate genomes generated using multiple sources of gene annotation. The database also contains a collection of annotated transcription factor binding sites (TFBSs) in evolutionary conserved and promoter elements. ECRbase currently includes human, rhesus macaque, dog, opossum, rat, mouse, chicken, frog, zebrafish, and fugu genomes. (taken from paper in Journal: Bioinformatics, November 7, 2006, pp. 122-124

  9. DOE Advanced Manufacturing Office EnPI V3.0 Webinar Transcription

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Advanced Manufacturing Office EnPI V3.0 Webinar Transcription December 11, 2012 Lindsay Southerland: Good Afternoon. My name is Lindsay Southerland and I'm with BCS, Incorporated. It is my pleasure to welcome you to this afternoon's webcast, which is sponsored by the U.S. Department of Energy's Advanced Manufacturing Office, or AMO for short. Today's presenters are Andre de Fontaine of AMO and Ashly Spevacek of the Project Performance Corporation. Ashly and Andre will provide an overview and

  10. Summary of Findings: Peer Review of the FY2000 GPRA Assumptions |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy 0 GPRA Assumptions Summary of Findings: Peer Review of the FY2000 GPRA Assumptions Summary of Findings: Peer Review of the FY2000 GPRA Assumptions, Report to National Renewable Energy Laboratory, March 1999. PDF icon Summary of Findings More Documents & Publications Summary of Findings: Peer Review of the FY2001 GPRA Assumptions Summary of Findings: Peer Review of the FY2003 GPRA Assumptions ITP Steel: Steel Industry Marginal Opportunity Study September 2005

  11. Summary of Findings: Peer Review of the FY2001 GPRA Assumptions |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy 1 GPRA Assumptions Summary of Findings: Peer Review of the FY2001 GPRA Assumptions Summary of Findings: Peer Review of the FY2001 GPRA Assumptions, Report to National Renewable Energy Laboratory, February 29, 2000. PDF icon Summary of Findings More Documents & Publications Summary of Findings: Peer Review of the FY2003 GPRA Assumptions Summary of Findings: Peer Review of the FY2000 GPRA Assumptions Appendix K - GPRA06 Weatherization and Intergovernmental Program

  12. Summary of Findings: Peer Review of the FY2003 GPRA Assumptions |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy 3 GPRA Assumptions Summary of Findings: Peer Review of the FY2003 GPRA Assumptions Summary of Findings: Peer Review of the FY '03 GPRA Assumptions, Report to National Renewable Energy Laboratory, Washington, D.C., April 3, 2002. PDF icon Summary of Findings More Documents & Publications Summary of Findings: Peer Review of the FY2001 GPRA Assumptions Summary of Findings: Peer Review of the FY2000 GPRA Assumptions Opportunities for Micropower and Fuel Cell/Gas Turbine

  13. Crystal structure of enterococcus faecalis sly A-like transcriptional factor.

    SciTech Connect (OSTI)

    Wu, R.; Zhang, R.; Zagnitko, O.; Dementieva, I.; Maltsev, N.; Watson, J. D.; Laskowski, R.; Gornicki, P.; Joachimiak, A.; Univ. of Chicago; European Bioinformatics Inst.

    2003-05-30

    The crystal structure of a SlyA transcriptional regulator at 1.6 {angstrom} resolution is presented, and structural relationships between members of the MarR/SlyA family are discussed. The SlyA family, which includes SlyA, Rap, Hor, and RovA proteins, is widely distributed in bacterial and archaeal genomes. Current evidence suggests that SlyA-like factors act as repressors, activators, and modulators of gene transcription. These proteins have been shown to up-regulate the expression of molecular chaperones, acid-resistance proteins, and cytolysin, and down-regulate several biosynthetic enzymes. The structure of SlyA from Enterococcus faecalis, determined as a part of an ongoing structural genomics initiative (www.mcsg.anl.gov), revealed the same winged helix DNA-binding motif that was recently found in the MarR repressor from Escherichia coli and the MexR repressor from Pseudomonas aeruginosa, a sequence homologue of MarR. Phylogenetic analysis of the MarR/SlyA family suggests that Sly is placed between the SlyA and MarR subfamilies and shows significant sequence similarity to members of both subfamilies.

  14. Reprogramming of human fibroblasts to pluripotent stem cells using mRNA of four transcription factors

    SciTech Connect (OSTI)

    Yakubov, Eduard; Rechavi, Gidi; Rozenblatt, Shmuel; Givol, David

    2010-03-26

    Reprogramming of differentiated cells into induced pluripotent cells (iPS) was accomplished in 2006 by expressing four, or less, embryonic stem cell (ESC)-specific transcription factors. Due to the possible danger of DNA damage and the potential tumorigenicity associated with such DNA damage, attempts were made to minimize DNA integration by the vectors involved in this process without complete success. Here we present a method of using RNA transfection as a tool for reprogramming human fibroblasts to iPS. We used RNA synthesized in vitro from cDNA of the same reprogramming four transcription factors. After transfection of the RNA, we show intracellular expression and nuclear localization of the respective proteins in at least 70% of the cells. We used five consecutive transfections to support continuous protein expression resulting in the formation of iPS colonies that express alkaline phosphatase and several ESC markers and that can be expanded. This method completely avoids DNA integration and may be developed to replace the use of DNA vectors in the formation of iPS.

  15. mRNA Transcript Abundance during Plant Growth and the Influence of Li+ Exposure

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Duff, M. C.; Kuhne, W. W.; Halverson, N. V.; Chang, C. -S.; Kitamura, E.; Hawthorn, L.; Martinez, N. E.; Stafford, C.; Milliken, C. E.; Caldwell, E. F.; et al

    2014-10-23

    Lithium (Li) toxicity in plants is, at a minimum, a function of Li+ concentration, exposure time, species and growth conditions. Most plant studies with Li+ focus on short-term acute exposures. This study examines short- and long-term effects of Li+ exposure in Arabidopsis with Li+ uptake studies and measured shoot mRNA transcript abundance levels in treated and control plants. Stress, pathogen-response and arabinogalactan protein genes were typically more up-regulated in older (chronic, low level) Li+-treatment plants and in the much younger plants from acute high-level exposures. The gene regulation behavior of high-level Li+ resembled prior studies due to its influence on:more »inositol synthesis, 1-aminocyclopropane-1-carboxylate synthases and membrane ion transport. In contrast, chronically-exposed plants had gene regulation responses that were indicative of pathogen, cold, and heavy-metal stress, cell wall degradation, ethylene production, signal transduction, and calcium-release modulation. Acute Li+ exposure phenocopies magnesium-deficiency symptoms and is associated with elevated expression of stress response genes that could lead to consumption of metabolic and transcriptional energy reserves and the dedication of more resources to cell development. In contrast, chronic Li+ exposure increases expression signal transduction genes. The identification of new Li+-sensitive genes and a gene-based “response plan” for acute and chronic Li+ exposure are delineated.« less

  16. mRNA Transcript Abundance during Plant Growth and the Influence of Li+ Exposure

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Duff, M. C.; Kuhne, W. W.; Halverson, N. V.; Chang, C. -S.; Kitamura, E.; Hawthorn, L.; Martinez, N. E.; Stafford, C.; Milliken, C. E.; Caldwell, E. F.; et al

    2014-10-23

    Lithium (Li) toxicity in plants is, at a minimum, a function of Li+ concentration, exposure time, species and growth conditions. Most plant studies with Li+ focus on short-term acute exposures. This study examines short- and long-term effects of Li+ exposure in Arabidopsis with Li+ uptake studies and measured shoot mRNA transcript abundance levels in treated and control plants. Stress, pathogen-response and arabinogalactan protein genes were typically more up-regulated in older (chronic, low level) Li+-treatment plants and in the much younger plants from acute high-level exposures. The gene regulation behavior of high-level Li+ resembled prior studies due to its influence on:more » inositol synthesis, 1-aminocyclopropane-1-carboxylate synthases and membrane ion transport. In contrast, chronically-exposed plants had gene regulation responses that were indicative of pathogen, cold, and heavy-metal stress, cell wall degradation, ethylene production, signal transduction, and calcium-release modulation. Acute Li+ exposure phenocopies magnesium-deficiency symptoms and is associated with elevated expression of stress response genes that could lead to consumption of metabolic and transcriptional energy reserves and the dedication of more resources to cell development. In contrast, chronic Li+ exposure increases expression signal transduction genes. The identification of new Li+-sensitive genes and a gene-based “response plan” for acute and chronic Li+ exposure are delineated.« less

  17. Oak Ridge Finds Ways to Remove K-25 Faster, Cheaper | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finds Ways to Remove K-25 Faster, Cheaper Oak Ridge Finds Ways to Remove K-25 Faster, Cheaper February 1, 2012 - 12:00pm Addthis Oak Ridge Finds Ways to Remove K-25 Faster, Cheaper Oak Ridge Finds Ways to Remove K-25 Faster, Cheaper Oak Ridge Finds Ways to Remove K-25 Faster, Cheaper Oak Ridge Finds Ways to Remove K-25 Faster, Cheaper OAK RIDGE, Tenn. - K-25, once the world's largest building under one roof, reflects less of its former title every day. Due to the partnership between the U.S

  18. Find Reports

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    regulations and permits, as well as other documents dealing with environmental issues. Many of these are available online in the EPRR database. You can also access the...

  19. Find Preprints

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    available via subscription and thus require Remote Access. Questions? 667-5809 Email Databases arXiv.org - open access eprints in Physics, Mathematics, Computer Science,...

  20. Oestradiol reduces Liver Receptor Homolog-1 mRNA transcript stability in breast cancer cell lines

    SciTech Connect (OSTI)

    Lazarus, Kyren A.; Environmental and Biotechnology Centre, Swinburne University, Hawthorn, Victoria 3122 ; Zhao, Zhe; Knower, Kevin C.; To, Sarah Q.; Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3168 ; Chand, Ashwini L.; Clyne, Colin D.

    2013-08-30

    Highlights: LRH-1 is an orphan nuclear receptor that regulates tumor proliferation. In breast cancer, high mRNA expression is associated with ER+ status. In ER?ve cells, despite very low mRNA, we found abundant LRH-1 protein. Our data show distinctly different LRH-1 protein isoforms in ER? and ER+ breast cancer cells. This is due to differences in LRH-1 mRNA and protein stability rates. -- Abstract: The expression of orphan nuclear receptor Liver Receptor Homolog-1 (LRH-1) is elevated in breast cancer and promotes proliferation, migration and invasion in vitro. LRH-1 expression is regulated by oestrogen (E{sub 2}), with LRH-1 mRNA transcript levels higher in oestrogen receptor ? (ER?) positive (ER+) breast cancer cells compared to ER? cells. However, the presence of LRH-1 protein in ER? cells suggests discordance between mRNA transcript levels and protein expression. To understand this, we investigated the impact of mRNA and protein stability in determining LRH-1 protein levels in breast cancer cells. LRH-1 transcript levels were significantly higher in ER+ versus ER? breast cancer cells lines; however LRH-1 protein was expressed at similar levels. We found LRH-1 mRNA and protein was more stable in ER? compared to ER+ cell lines. The tumor-specific LRH-1 variant isoform, LRH-1v4, which is highly responsive to E{sub 2}, showed increased mRNA stability in ER? versus ER+ cells. In addition, in MCF-7 and T47-D cell lines, LRH-1 total mRNA stability was reduced with E{sub 2} treatment, this effect mediated by ER?. Our data demonstrates that in ER? cells, increased mRNA and protein stability contribute to the abundant protein expression levels. Expression and immunolocalisation of LRH-1 in ER? cells as well as ER? tumors suggests a possible role in the development of ER? tumors. The modulation of LRH-1 bioactivity may therefore be beneficial as a treatment option in both ER? and ER+ breast cancer.

  1. On-chip single-copy real-time reverse-transcription PCR in isolated picoliter droplets

    SciTech Connect (OSTI)

    Beer, N R; Wheeler, E; Lee-Houghton, L; Watkins, N; Nasarabadi, S; Hebert, N; Leung, P; Arnold, D; Bailey, C; Colston, B

    2007-12-19

    The first lab-on-chip system for picoliter droplet generation and RNA isolation, followed by reverse transcription, and PCR amplification with real-time fluorescence detection in the trapped droplets has been developed. The system utilized a shearing T-junction in a fused silica device to generate a stream of monodisperse picoliter-scale droplets that were isolated from the microfluidic channel walls and each other by the oil phase carrier. An off-chip valving system stopped the droplets on-chip, allowing thermal cycling for reverse transcription and subsequent PCR amplification without droplet motion. This combination of the established real-time reverse transcription-PCR assay with digital microfluidics is ideal for isolating single-copy RNA and virions from a complex environment, and will be useful in viral discovery and gene-profiling applications.

  2. Fact #872: May 11, 2015 Study Finds More than 60% of Millennials...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Fact 872: May 11, 2015 Study Finds More than 60% of Millennials and Generation Xers Use the Internet to Find a Car Dealer While Less than Half of Baby Boomers Did - Dataset Excel ...

  3. Alaska Oil and Gas Finding of Best Interest | Open Energy Information

    Open Energy Info (EERE)

    Oil and Gas Finding of Best Interest Jump to: navigation, search OpenEI Reference LibraryAdd to library Web Site: Alaska Oil and Gas Finding of Best Interest Author Alaska...

  4. New DOE Report Finds Wind Power Can Serve as Cost-Effective Long...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Finds Wind Power Can Serve as Cost-Effective Long-Term Hedge Against Natural Gas Price Increases New DOE Report Finds Wind Power Can Serve as Cost-Effective Long-Term Hedge Against ...

  5. Report on Hydrogen Storage Panel Findings in DOE-BES Sponsored...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Hydrogen Storage Panel Findings in DOE-BES Sponsored Workshop on Basic Research for Hydrogen Production, Storage and Use Report on Hydrogen Storage Panel Findings in DOE-BES...

  6. EA-1800: Notice of Availability of the Finding of No Significant...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Notice of Availability of the Finding of No Significant Impact Monarch Warren County Wind Turbine Project The U.S. Department of Energy (DOE) has issued a Finding of No...

  7. Fact #872: May 11, 2015 Study Finds More than 60% of Millennials...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Millennials and Generation Xers Use the Internet to Find a Car Dealer While Less than Half of Baby Boomers Did Fact 872: May 11, 2015 Study Finds More than 60% of Millennials ...

  8. EA-1941: Finding of No Significant Impact (FONSI) | Department of Energy

    Office of Environmental Management (EM)

    Finding of No Significant Impact (FONSI) EA-1941: Finding of No Significant Impact (FONSI) BPA issued a Finding of No Significant Impact for proposed improvements to 13 miles of access roads for its existing 115-kV Boyer-Tillamook No. 1 Transmission Line. PDF icon EA-1941-FONSI-2014.pdf More Documents & Publications EA-1941: Final Environmental Assessment

  9. POSTED BY JOB NAME FIELD OFFICE U.S. DEPARTMENT OF ENERGY ADP TRANSCRIPTION SHEET

    Energy Savers [EERE]

    1 1 1 1 1 1 1 POSTED BY JOB NAME FIELD OFFICE U.S. DEPARTMENT OF ENERGY ADP TRANSCRIPTION SHEET INTERNAL PROJECT TRANSFERS (See instructions for provisions regarding confidentiality.) ACT. CODE PROC. CODE 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 1 SHIPPER RIS TRAN. CODE TO PROJECT NUMBER RECEIVER RIS INTERNAL

  10. POSTED BY JOB NAME FIELD OFFICE U.S. DEPARTMENT OF ENERGY ADP TRANSCRIPTION SHEET

    National Nuclear Security Administration (NNSA)

    1 1 1 1 1 1 1 POSTED BY JOB NAME FIELD OFFICE U.S. DEPARTMENT OF ENERGY ADP TRANSCRIPTION SHEET INTERNAL PROJECT TRANSFERS (See instructions for provisions regarding confidentiality.) ACT. CODE PROC. CODE 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 1 SHIPPER RIS TRAN. CODE TO PROJECT NUMBER RECEIVER RIS INTERNAL

  11. Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus

    DOE Patents [OSTI]

    Dai, Ziyu; Lasure, Linda L.; Magnuson, Jon K.

    2008-11-11

    The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus.

  12. Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus

    DOE Patents [OSTI]

    Dai, Ziyu; Lasure, Linda L; Magnuson, Jon K

    2014-05-27

    The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus.

  13. Isolated Fungal Promoters and Gene Transcription Terminators and Methods of Protein and Chemical Production in a Fungus

    DOE Patents [OSTI]

    Dai, Ziyu; Lasure, Linda L.; Magnuson, Jon K.

    2008-11-11

    The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus.

  14. An integrated approach to reconstructing genome-scale transcriptional regulatory networks

    SciTech Connect (OSTI)

    Imam, Saheed; Noguera, Daniel R.; Donohue, Timothy J.; Leslie, Christina

    2015-02-27

    Transcriptional regulatory networks (TRNs) program cells to dynamically alter their gene expression in response to changing internal or environmental conditions. In this study, we develop a novel workflow for generating large-scale TRN models that integrates comparative genomics data, global gene expression analyses, and intrinsic properties of transcription factors (TFs). An assessment of this workflow using benchmark datasets for the well-studied ?-proteobacterium Escherichia coli showed that it outperforms expression-based inference approaches, having a significantly larger area under the precision-recall curve. Further analysis indicated that this integrated workflow captures different aspects of the E. coli TRN than expression-based approaches, potentially making them highly complementary. We leveraged this new workflow and observations to build a large-scale TRN model for the ?-Proteobacterium Rhodobacter sphaeroides that comprises 120 gene clusters, 1211 genes (including 93 TFs), 1858 predicted protein-DNA interactions and 76 DNA binding motifs. We found that ~67% of the predicted gene clusters in this TRN are enriched for functions ranging from photosynthesis or central carbon metabolism to environmental stress responses. We also found that members of many of the predicted gene clusters were consistent with prior knowledge in R. sphaeroides and/or other bacteria. Experimental validation of predictions from this R. sphaeroides TRN model showed that high precision and recall was also obtained for TFs involved in photosynthesis (PpsR), carbon metabolism (RSP_0489) and iron homeostasis (RSP_3341). In addition, this integrative approach enabled generation of TRNs with increased information content relative to R. sphaeroides TRN models built via other approaches. We also show how this approach can be used to simultaneously produce TRN models for each related organism used in the comparative genomics analysis. Our results highlight the advantages of integrating comparative genomics of closely related organisms with gene expression data to assemble large-scale TRN models with high-quality predictions.

  15. An integrated approach to reconstructing genome-scale transcriptional regulatory networks

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Imam, Saheed; Noguera, Daniel R.; Donohue, Timothy J.; Leslie, Christina

    2015-02-27

    Transcriptional regulatory networks (TRNs) program cells to dynamically alter their gene expression in response to changing internal or environmental conditions. In this study, we develop a novel workflow for generating large-scale TRN models that integrates comparative genomics data, global gene expression analyses, and intrinsic properties of transcription factors (TFs). An assessment of this workflow using benchmark datasets for the well-studied γ-proteobacterium Escherichia coli showed that it outperforms expression-based inference approaches, having a significantly larger area under the precision-recall curve. Further analysis indicated that this integrated workflow captures different aspects of the E. coli TRN than expression-based approaches, potentially making themmore » highly complementary. We leveraged this new workflow and observations to build a large-scale TRN model for the α-Proteobacterium Rhodobacter sphaeroides that comprises 120 gene clusters, 1211 genes (including 93 TFs), 1858 predicted protein-DNA interactions and 76 DNA binding motifs. We found that ~67% of the predicted gene clusters in this TRN are enriched for functions ranging from photosynthesis or central carbon metabolism to environmental stress responses. We also found that members of many of the predicted gene clusters were consistent with prior knowledge in R. sphaeroides and/or other bacteria. Experimental validation of predictions from this R. sphaeroides TRN model showed that high precision and recall was also obtained for TFs involved in photosynthesis (PpsR), carbon metabolism (RSP_0489) and iron homeostasis (RSP_3341). In addition, this integrative approach enabled generation of TRNs with increased information content relative to R. sphaeroides TRN models built via other approaches. We also show how this approach can be used to simultaneously produce TRN models for each related organism used in the comparative genomics analysis. Our results highlight the advantages of integrating comparative genomics of closely related organisms with gene expression data to assemble large-scale TRN models with high-quality predictions.« less

  16. Ligand-specific transcriptional mechanisms underlie aryl hydrocarbon receptor-mediated developmental toxicity of oxygenated PAHs

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Goodale, B. C.; Geisel School of Medicine at Dartmouth, Hanover, NH; La Du, J.; Tilton, S. C.; Pacific Northwest National Lab.; Sullivan, C. M.; Bisson, W. H.; Waters, K. M.; Tanguay, R. L.

    2015-07-03

    Polycyclic aromatic hydrocarbons (PAHs) are priority environmental contaminants that exhibit mutagenic, carcinogenic, proinflammatory, and teratogenic properties. Oxygen-substituted PAHs (OPAHs) are formed during combustion processes and via phototoxidation and biological degradation of parent (unsubstituted) PAHs. Despite their prevalence both in contaminated industrial sites and in urban air, OPAH mechanisms of action in biological systems are relatively understudied. Like parent PAHs, OPAHs exert structure-dependent mutagenic activities and activation of the aryl hydrocarbon receptor (AHR) and cytochrome p450 metabolic pathway. Four-ring OPAHs 1,9-benz-10-anthrone (BEZO) and benz(a)anthracene-7,12-dione (7,12-B[a]AQ) cause morphological aberrations and induce markers of oxidative stress in developing zebrafish with similar potency, butmore » only 7,12-B[a]AQ induces robust Cyp1a protein expression. We investigated the role of the AHR in mediating the toxicity of BEZO and 7,12-B[a]AQ, and found that knockdown of AHR2 rescued developmental effects caused by both compounds. Using RNA-seq and molecular docking, we identified transcriptional responses that precede developmental toxicity induced via differential interaction with AHR2. Redox-homeostasis genes were affected similarly by these OPAHs, while 7,12-B[a]AQ preferentially activated phase 1 metabolism and BEZO uniquely decreased visual system genes. Analysis of biological functions and upstream regulators suggests that BEZO is a weak AHR agonist, but interacts with other transcriptional regulators to cause developmental toxicity in an AHR-dependent manner. Furthermore, identifying ligand-dependent AHR interactions and signaling pathways is essential for understanding toxicity of this class of environmentally relevant compounds.« less

  17. Fact #872: May 11, 2015 Study Finds More than 60% of Millennials and

    Office of Environmental Management (EM)

    Generation Xers Use the Internet to Find a Car Dealer While Less than Half of Baby Boomers Did | Department of Energy 72: May 11, 2015 Study Finds More than 60% of Millennials and Generation Xers Use the Internet to Find a Car Dealer While Less than Half of Baby Boomers Did Fact #872: May 11, 2015 Study Finds More than 60% of Millennials and Generation Xers Use the Internet to Find a Car Dealer While Less than Half of Baby Boomers Did According to an AutoTrader-commissioned study of people

  18. AS-2, a novel inhibitor of p53-dependent apoptosis, prevents apoptotic mitochondrial dysfunction in a transcription-independent manner and protects mice from a lethal dose of ionizing radiation

    SciTech Connect (OSTI)

    Morita, Akinori; Ariyasu, Shinya; Wang, Bing; Asanuma, Tetsuo; Onoda, Takayoshi; Sawa, Akiko; Tanaka, Kaoru; Takahashi, Ippei; Togami, Shotaro; Nenoi, Mitsuru; Inaba, Toshiya; Aoki, Shin

    2014-08-08

    Highlights: A bidentate HQ derivative, AS-2, suppresses p53-dependent apoptosis by DNA damage. AS-2 does not significantly affect nuclear p53 response. UV-excited blue emission of AS-2 clearly showed its extranuclear localization. AS-2 prevents mitochondrial dysfunction despite the increase of mitochondrial p53. AS-2 protects mice from a radiation dose that causes lethal hematopoietic syndrome. - Abstract: In a previous study, we reported that some tetradentate zinc(II) chelators inhibit p53 through the denaturation of its zinc-requiring structure but a chelator, Bispicen, a potent inhibitor of in vitro apoptosis, failed to show any efficient radioprotective effect against irradiated mice because the toxicity of the chelator to mice. The unsuitability of using tetradentate chelators as radioprotectors prompted us to undertake a more extensive search for p53-inhibiting agents that are weaker zinc(II) chelators and therefore less toxic. Here, we show that an 8-hydroxyquinoline (8HQ) derivative, AS-2, suppresses p53-dependent apoptosis through a transcription-independent mechanism. A mechanistic study using cells with different p53 characteristics revealed that the suppressive effect of AS-2 on apoptosis is specifically mediated through p53. In addition, AS-2 was less effective in preventing p53-mediated transcription-dependent events than pifithrin-? (PFT?), an inhibitor of transcription-independent apoptosis by p53. Fluorescence visualization of the extranuclear distribution of AS-2 also supports that it is ineffective on the transcription-dependent pathway. Further investigations revealed that AS-2 suppressed mitochondrial apoptotic events, such as the mitochondrial release of intermembrane proteins and the loss of mitochondrial membrane potential, although AS-2 resulted in an increase in the mitochondrial translocation of p53 as opposed to the decrease of cytosolic p53, and did not affect the apoptotic interaction of p53 with Bcl-2. AS-2 also protected mice that had been exposed to a lethal dose of ionizing radiation. Our findings indicate that some types of bidentate 8HQ chelators could serve as radioprotectors with no substantial toxicity in vivo.

  19. Portsmouth Gaseous Diffusion Plant Director's Final Findings and Orders, October 4, 1995

    Office of Environmental Management (EM)

    Gaseous Diffusion Plant Director's Final Findings and Orders, October 4, 1995 BEFORE THE OHIO ENVIRONMENTAL PROTECTION AGENCY In the Matter Of: United States Department of Energy : Director's Final Portsmouth Gaseous Diffusion Plant : Findings and Orders P.O. Box 700 : Piketon, Ohio 45661-0700 : Respondent It is hereby agreed by and among the parties hereto as follows: Table of Contents I. Jurisdiction II. Parties Bound III. Definitions IV. Findings of Fact V. Orders VI. Limitations of

  20. EA-1931: Finding of No Significant Impact and Mitigation Action Plan |

    Energy Savers [EERE]

    Department of Energy 1: Finding of No Significant Impact and Mitigation Action Plan EA-1931: Finding of No Significant Impact and Mitigation Action Plan Keeler to Tillamook Transmission Line Rebuild Project, Washington and Tillamook Counties, Oregon Bonneville Power Administration issued a finding of no significant impact and a mitigation action plan for the proposed rebuild of the Keeler-Forest Grove and Forest Grove-Tillamook 115-kilovolt (kV) transmission lines between the cities of

  1. EA-1946: Finding of No Significant Impact and Mitigation Action Plan |

    Energy Savers [EERE]

    Department of Energy 6: Finding of No Significant Impact and Mitigation Action Plan EA-1946: Finding of No Significant Impact and Mitigation Action Plan Salem-Albany Transmission Line Rebuild Project; Polk, Benton, Marion, and Linn Counties, Oregon Bonneville Power Administration issued a finding of no significant impact and mitigation action plan for the proposed rebuild of the 24-mile Salem-Albany No. 1 and 28-mile Salem-Albany No. 2 transmission lines between Salem and Albany, Oregon. PDF

  2. EA-1950: Finding of No Significant Impact and Mitigation Action Plan |

    Energy Savers [EERE]

    Department of Energy 0: Finding of No Significant Impact and Mitigation Action Plan EA-1950: Finding of No Significant Impact and Mitigation Action Plan Grand Coulee-Creston Transmission Line Rebuild; Grant and Lincoln Counties, Washington Bonneville Power Administration issued a finding of no significant impact and a mitigation action plan for rebuilding approximately 28 miles of the Grand Coulee-Creston No. 1 115-kilovolt (kV) transmission line between Coulee Dam in Grant County and

  3. EA-1973: Finding of No Significant Impact and Mitigation Action Plan |

    Energy Savers [EERE]

    Department of Energy 3: Finding of No Significant Impact and Mitigation Action Plan EA-1973: Finding of No Significant Impact and Mitigation Action Plan Kootenai River Restoration at Bonners Ferry, Boundary County, Idaho Bonneville Power Administration issued a Finding of No Significant Impact and Mitigation Action Plan for funding the Kootenai Tribe of Idaho to restore portions of the Kootenai River near the town of Bonners Ferry, Idaho. The project involves installing structures on the

  4. Finding of No Significant Impact, Federal Register, 73 FR 2017, January 11,

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    2008 | Department of Energy Finding of No Significant Impact, Federal Register, 73 FR 2017, January 11, 2008 Finding of No Significant Impact, Federal Register, 73 FR 2017, January 11, 2008 Document details the finding of no significant impact of the Energy-Efficient Performance Requirements for New Federal Commercial and Residential Buildings, which was published in the Federal Register on January 11, 2008. PDF icon 73_fr_2017.pdf More Documents & Publications Special Environmental

  5. Focus Series: Program Finds Community "Readiness" Is the Key to More

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Retrofits | Department of Energy Focus Series: Program Finds Community "Readiness" Is the Key to More Retrofits Focus Series: Program Finds Community "Readiness" Is the Key to More Retrofits Focus Series: Program Finds Community "Readiness" Is the Key to More Retrofits, a document posted by the U.S. Department of Energy's (DOE'S) Better Buildings Neighborhood Program. PDF icon Focus Series: Michigan More Documents & Publications Better Buildings Network View

  6. Fernald Environmental Management Project Director's Final Findings and Orders, October 4, 1995

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Fernald Environmental Management Project Director's Final Findings and Orders, Octobe.. Page 1 of 17 EM Home | Regulatory Compliance | Environmental Compliance Agreements Fernald Environmental Management Project Director's Final Findings and Orders, October 4, 1995 BEFORE THE OHIO ENVIRONMENTAL PROTECTION AGENCY In the Matter Of: United States Department of Energy : Director's Final Fernald Environmental Management Project : Findings and Orders P.O. Box 389705 : Cincinnati, Ohio 45239 :

  7. Business Case for Small Modular Reactors Report on Findings | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Business Case for Small Modular Reactors Report on Findings Business Case for Small Modular Reactors Report on Findings This study assesses the market for SMRs and develops a business case to identify incentives, policies, and programs that can be effectively implemented and have significant impact on the commercialization of SMRs. PDF icon Business Case for Small Modular Reactors Report on Findings to the U.S. Department of Energy More Documents & Publications A Strategic

  8. Tour of Zero: Helping Customers Find Better Homes at Lower Costs Webinar

    Energy Savers [EERE]

    (Text Version) | Department of Energy Tour of Zero: Helping Customers Find Better Homes at Lower Costs Webinar (Text Version) Tour of Zero: Helping Customers Find Better Homes at Lower Costs Webinar (Text Version) Below is the text version of the webinar Tour of Zero: Helping Customers Find Better Homes at Lower Costs, presented in October 2015. Watch the presentation. Lindsay Parker: Hi, everyone. Welcome to the Department of Energy Zero Energy Ready Home technical training webinar series.

  9. EA-1951: Finding of No Significant Impact and Mitigation Action Plan |

    Energy Savers [EERE]

    Department of Energy 1951: Finding of No Significant Impact and Mitigation Action Plan EA-1951: Finding of No Significant Impact and Mitigation Action Plan Midway-Moxee Rebuild and Midway-Grandview Upgrade Transmission Line Project; Benton and Yakima Counties, Washington Bonneville Power Administration issued a Finding of No Significant Impact and Mitigation Action Plan for the proposed rebuilding of the 34-mile Midway-Moxee transmission line and the proposed rebuilding and upgrading of the

  10. Focus Series: Program Finds Community "Readiness" Is the Key to More

    Energy Savers [EERE]

    Retrofits | Department of Energy Program Finds Community "Readiness" Is the Key to More Retrofits Focus Series: Program Finds Community "Readiness" Is the Key to More Retrofits Focus Series: Program Finds Community "Readiness" Is the Key to More Retrofits, a document posted by the U.S. Department of Energy's (DOE'S) Better Buildings Neighborhood Program. PDF icon Focus Series: Michigan More Documents & Publications Community Readiness Assessments Better

  11. Using regulations.gov to find dockets and documents | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Using regulations.gov to find dockets and documents Using regulations.gov to find dockets and documents A document from the U.S. Department of Energy's Office of Energy Efficiency and Renewable Energy, Building Technologies Office, explaining how to use Regulations.gov to find dockets and documents. PDF icon as_accessing_documents.pdf More Documents & Publications Regulations.gov - Proposed Rule Document ISSUANCE 2015-08-21: Energy Conservation Program for Consumer Products: Energy

  12. EIS-0409: Record of Decision and Statement of Findings | Department of

    Office of Environmental Management (EM)

    Energy 09: Record of Decision and Statement of Findings EIS-0409: Record of Decision and Statement of Findings Kemper County IGCC Project, Kemper County, Mississippi Record of Decision for an environmental impact statement associated with a proposed project using Integrated Gasification Combined Cycle Technology in Kemper County, Mississippi. PDF icon Record of Decision and Statement of Findings; Kemper County IGCC Project, Kemper County, Mississippi, EIS-0409, (August 2010) (75 FR 51248)

  13. How to find fees, taxes, and other charges built into rate in...

    Open Energy Info (EERE)

    and other charges built into rate in different locations Home > Groups > Utility Rate Hi, I am looking to find the rate charges beyond demand, generation, and transmission that...

  14. EA-1774-S1: Final Environmental Assessment and Finding of No...

    Office of Environmental Management (EM)

    EA-1774-S1: Final Environmental Assessment and Finding of No Significant Impact Energy Conservation Program: Energy Conservation Standards for Direct Heating Equipment (November ...

  15. Six Places to Find Help with Your Energy Costs | Department of...

    Energy Savers [EERE]

    and Efficiency (DSIRE). Here you'll find a comprehensive listing of available rebates, loans, grants, and tax incentives for renewable energy technologies and energy...

  16. Clark Fork River Delta Restoration Project 1 Finding of No Significant...

    Broader source: Energy.gov (indexed) [DOE]

    findings for its proposal to provide partial funding to the Idaho Department of Fish and Game's (IDFG) Clark Fork River Delta Restoration Project. The project would involve...

  17. EA-1815: DOE Notice of Availability of the Finding of No Significant...

    Broader source: Energy.gov (indexed) [DOE]

    environmental impacts associated with the: Cuyahoga County Agricultural Society Wind Energy Project; Berea, Cuyahoga County, Ohio. Notice of Availability of a Finding of No...

  18. Idaho IC 61-1701, Legislative Purposes and Findings for the Siting...

    Open Energy Info (EERE)

    701, Legislative Purposes and Findings for the Siting of Certain Electrical Transmission Facilities Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal...

  19. How Do You Find Thermal Leaks in Your Home? | Department of Energy

    Energy Savers [EERE]

    Find Thermal Leaks in Your Home? How Do You Find Thermal Leaks in Your Home? March 31, 2011 - 7:30am Addthis On Monday, John told you about the thermal leak detector he purchased to help him find and seal leaks in his home. A thermal leak detector can be a great tool to help you find leaks in your own home, but it's not your only option. In addition to tools like this, you can also use some of our tips on do-it-yourself energy assessments, or you could get a professional energy assessment. How

  20. DOE/EA-1976 FINDING OF NO SIGNIFICANT IMPACT FOR PROPOSED CNG...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    DOEEA-1976 FINDING OF NO SIGNIFICANT IMPACT FOR PROPOSED CNG PROJECT REGARDING EMERA CNG, LLC APPLICATION SEEKING DEPARTMENT OF ENERGY AUTHORIZATION TO EXPORT COMPRESSED NATURAL ...

  1. NREL Tool Finds Effective Behind-the-Meter Energy Storage Configuratio...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Tool Finds Effective Behind-the-Meter Energy Storage Configurations Small battery systems can offer attractive return on investment March 9, 2015 The Energy Department's (DOE) ...

  2. Automated genomic context analysis and experimental validation platform for discovery of prokaryote transcriptional regulator functions

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Martí-Arbona, Ricardo; Mu, Fangping; Nowak-Lovato, Kristy L.; Wren, Melinda S.; Unkefer, Clifford J.; Unkefer, Pat J.

    2014-12-18

    The clustering of genes in a pathway and the co-location of functionally related genes is widely recognized in prokaryotes. We used these characteristics to predict the metabolic involvement for a Transcriptional Regulator (TR) of unknown function, identified and confirmed its biological activity. A software tool that identifies the genes encoded within a defined genomic neighborhood for the subject TR and its homologs was developed. The output lists of genes in the genetic neighborhoods, their annotated functions, the reactants/products, and identifies the metabolic pathway in which the encoded-proteins function. When a set of TRs of known function was analyzed, we observedmore » that their homologs frequently had conserved genomic neighborhoods that co-located the metabolically related genes regulated by the subject TR. We postulate that TR effectors are metabolites in the identified pathways; indeed the known effectors were present. We analyzed Bxe_B3018 from Burkholderia xenovorans, a TR of unknown function and predicted that this TR was related to the glycine, threonine and serine degradation. We tested the binding of metabolites in these pathways and for those that bound, their ability to modulate TR binding to its specific DNA operator sequence. Using rtPCR, we confirmed that methylglyoxal was an effector of Bxe_3018.« less

  3. Automated genomic context analysis and experimental validation platform for discovery of prokaryote transcriptional regulator functions

    SciTech Connect (OSTI)

    Mart-Arbona, Ricardo; Mu, Fangping; Nowak-Lovato, Kristy L.; Wren, Melinda S.; Unkefer, Clifford J.; Unkefer, Pat J.

    2014-12-18

    The clustering of genes in a pathway and the co-location of functionally related genes is widely recognized in prokaryotes. We used these characteristics to predict the metabolic involvement for a Transcriptional Regulator (TR) of unknown function, identified and confirmed its biological activity. A software tool that identifies the genes encoded within a defined genomic neighborhood for the subject TR and its homologs was developed. The output lists of genes in the genetic neighborhoods, their annotated functions, the reactants/products, and identifies the metabolic pathway in which the encoded-proteins function. When a set of TRs of known function was analyzed, we observed that their homologs frequently had conserved genomic neighborhoods that co-located the metabolically related genes regulated by the subject TR. We postulate that TR effectors are metabolites in the identified pathways; indeed the known effectors were present. We analyzed Bxe_B3018 from Burkholderia xenovorans, a TR of unknown function and predicted that this TR was related to the glycine, threonine and serine degradation. We tested the binding of metabolites in these pathways and for those that bound, their ability to modulate TR binding to its specific DNA operator sequence. Using rtPCR, we confirmed that methylglyoxal was an effector of Bxe_3018.

  4. Global transcriptional, physiological and metabolite analyses of Desulfovibrio vulgaris Hildenborough responses to salt adaptation

    SciTech Connect (OSTI)

    He, Z.; Zhou, A.; Baidoo, E.; He, Q.; Joachimiak, M. P.; Benke, P.; Phan, R.; Mukhopadhyay, A.; Hemme, C.L.; Huang, K.; Alm, E.J.; Fields, M.W.; Wall, J.; Stahl, D.; Hazen, T.C.; Keasling, J.D.; Arkin, A.P.; Zhou, J.

    2009-12-01

    The response of Desulfovibrio vulgaris Hildenborough to salt adaptation (long-term NaCl exposure) was examined by physiological, global transcriptional, and metabolite analyses. The growth of D. vulgaris was inhibited by high levels of NaCl, and the growth inhibition could be relieved by the addition of exogenous amino acids (e.g., glutamate, alanine, tryptophan) or yeast extract. Salt adaptation induced the expression of genes involved in amino acid biosynthesis and transport, electron transfer, hydrogen oxidation, and general stress responses (e.g., heat shock proteins, phage shock proteins, and oxidative stress response proteins). Genes involved in carbon metabolism, cell motility, and phage structures were repressed. Comparison of transcriptomic profiles of D. vulgaris responses to salt adaptation with those of salt shock (short-term NaCl exposure) showed some similarity as well as a significant difference. Metabolite assays showed that glutamate and alanine were accumulated under salt adaptation, suggesting that they may be used as osmoprotectants in D. vulgaris. A conceptual model is proposed to link the observed results to currently available knowledge for further understanding the mechanisms of D. vulgaris adaptation to elevated NaCl.

  5. Leptin upregulates telomerase activity and transcription of human telomerase reverse transcriptase in MCF-7 breast cancer cells

    SciTech Connect (OSTI)

    Ren, He; Zhao, Tiansuo; Wang, Xiuchao; Gao, Chuntao; Wang, Jian; Yu, Ming; Hao, Jihui

    2010-03-26

    The aim was to analyze the mechanism of leptin-induced activity of telomerase in MCF-7 breast cancer cells. We found that leptin activated telomerase in a dose-dependent manner; leptin upregulated the expression of Human Telomerase Reverse Transcriptase (hTERT) at mRNA and protein levels; blockade of signal transducer and activator of transcription 3 (STAT3) phosphorylation significantly counteracted leptin-induced hTERT transcription and protein expression; chromatin immunoprecipitation analysis showed that leptin enhanced the binding of STAT3 to the hTERT promoter. This study uncovers a new mechanism of the proliferative effect of leptin on breast cancer cells and provides a new explanation of obesity-related breast cancer.

  6. Experimental Definition and Validation of Protein Coding Transcripts in Chlamydomonas reinhardtii

    SciTech Connect (OSTI)

    Kourosh Salehi-Ashtiani; Jason A. Papin

    2012-01-13

    Algal fuel sources promise unsurpassed yields in a carbon neutral manner that minimizes resource competition between agriculture and fuel crops. Many challenges must be addressed before algal biofuels can be accepted as a component of the fossil fuel replacement strategy. One significant challenge is that the cost of algal fuel production must become competitive with existing fuel alternatives. Algal biofuel production presents the opportunity to fine-tune microbial metabolic machinery for an optimal blend of biomass constituents and desired fuel molecules. Genome-scale model-driven algal metabolic design promises to facilitate both goals by directing the utilization of metabolites in the complex, interconnected metabolic networks to optimize production of the compounds of interest. Using Chlamydomonas reinhardtii as a model, we developed a systems-level methodology bridging metabolic network reconstruction with annotation and experimental verification of enzyme encoding open reading frames. We reconstructed a genome-scale metabolic network for this alga and devised a novel light-modeling approach that enables quantitative growth prediction for a given light source, resolving wavelength and photon flux. We experimentally verified transcripts accounted for in the network and physiologically validated model function through simulation and generation of new experimental growth data, providing high confidence in network contents and predictive applications. The network offers insight into algal metabolism and potential for genetic engineering and efficient light source design, a pioneering resource for studying light-driven metabolism and quantitative systems biology. Our approach to generate a predictive metabolic model integrated with cloned open reading frames, provides a cost-effective platform to generate metabolic engineering resources. While the generated resources are specific to algal systems, the approach that we have developed is not specific to algae and can be readily expanded to other microbial systems as well as higher plants and animals.

  7. Expression of barley SUSIBA2 transcription factor yields high-starch low-methane rice

    SciTech Connect (OSTI)

    Su, J.; Hu, C.; Yan, X.; Jin, Y.; Chen, Z.; Guan, Q.; Wang, Y.; Zhong, D.; Jansson, Georg C.; Wang, F.; Schnrer, Anna; Sun, Chuanxin

    2015-07-22

    Atmospheric methane is the second most important greenhouse gas after carbon dioxide, and is responsible for about 20% of the global warming effect since pre-industrial times. Rice paddies are the largest anthropogenic methane source and produce 717% of atmospheric methane. Warm waterlogged soil and exuded nutrients from rice roots provide ideal conditions for methanogenesis in paddies with annual methane emissions of 25100-million tonnes. This scenario will be exacerbated by an expansion in rice cultivation needed to meet the escalating demand for food in the coming decades4. There is an urgent need to establish sustainable technologies for increasing rice production while reducing methane fluxes from rice paddies. However, ongoing efforts for methane mitigation in rice paddies are mainly based on farming practices and measures that are difficult to implement5. Despite proposed strategies to increase rice productivity and reduce methane emissions4,6, no high-starch low-methane-emission rice has been developed. Here we show that the addition of a single transcription factor gene, barley SUSIBA2, conferred a shift of carbon flux to SUSIBA2 rice, favouring the allocation of photosynthates to aboveground biomass over allocation to roots. The altered allocation resulted in an increased biomass and starch content in the seeds and stems, and suppressed methanogenesis, possibly through a reduction in root exudates. Three-year field trials in China demonstrated that the cultivation of SUSIBA2 rice was associated with a significant reduction in methane emissions and a decrease in rhizospheric methanogen levels. SUSIBA2 rice offers a sustainable means of providing increased starch content for food production while reducing greenhouse gas emissions from rice cultivation. Approaches to increase rice productivity and reduce methane emissions as seen in SUSIBA2 rice may be particularly beneficial in a future climate with rising temperatures resulting in increased methane emissions from paddies.

  8. Comparative phosphoproteomics reveals components of host cell invasion and post-transcriptional regulation during Francisella infection

    SciTech Connect (OSTI)

    Nakayasu, Ernesto S.; Tempel, Rebecca; Cambronne, Xiaolu A.; Petyuk, Vladislav A.; Jones, Marcus B.; Gritsenko, Marina A.; Monroe, Matthew E.; Yang, Feng; Smith, Richard D.; Adkins, Joshua N.; Heffron, Fred

    2013-09-22

    Francisella tularensis is a facultative intracellular bacterium that causes the deadly disease tularemia. Most evidence suggests that Francisella is not well recognized by the innate immune system that normally leads to cytokine expression and cell death. In previous work, we identified new bacterial factors that were hyper-cytotoxic to macrophages. Four of the identified hyper-cytotoxic strains (lpcC, manB, manC and kdtA) had an impaired lipopolysaccharide (LPS) synthesis and produced an exposed lipid A lacking the O-antigen. These mutants were not only hyper-cytotoxic but also were phagocytosed at much higher rates compared to the wild type parent strain. To elucidate the cellular signaling underlying this enhanced phagocytosis and cell death, we performed a large-scale comparative phosphoproteomic analysis of cells infected with wild-type and delta-lpcC F. novicida. Our data suggest that not only actin but also intermediate filaments and microtubules are important for F. novicida entry into the host cells. In addition, we observed differential phosphorylation of tristetraprolin (TTP), a key component of the mRNA-degrading machinery that controls the expression of a variety of genes including many cytokines. Infection with the delta-lpcC mutant induced the hyper-phosphorylation and inhibition of TTP, leading to the production of cytokines such as IL-1beta and TNF-alpha which may kill the host cells by triggering apoptosis. Together, our data provide new insights for Francisella invasion and a post-transcriptional mechanism that prevents the expression of host immune response factors that controls infection by this pathogen.

  9. HPV-18 E2circumflexE4 chimera: 2 new spliced transcripts and proteins induced by keratinocyte differentiation

    SciTech Connect (OSTI)

    Tan, Chye Ling; Gunaratne, Jayantha; Lai, Deborah; Carthagena, Laetitia; Wang, Qian; Xue, Yue Zhen; Quek, Ling Shih; Doorbar, John; Bachelerie, Francoise; Thierry, Francoise; Bellanger, Sophie

    2012-07-20

    The Human Papillomavirus (HPV) E4 is known to be synthesized as an E1circumflexE4 fusion resulting from splice donor and acceptor sites conserved across HPV types. Here we demonstrate the existence of 2 HPV-18 E2circumflexE4 transcripts resulting from 2 splice donor sites in the 5 Prime part of E2, while the splice acceptor site is the one used for E1circumflexE4. Both E2circumflexE4 transcripts are up-regulated by keratinocyte differentiation in vitro and can be detected in clinical samples containing low-grade HPV-18-positive cells from Pap smears. They give rise to two fusion proteins in vitro, E2circumflexE4-S and E2circumflexE4-L. Whereas we could not differentiate E2circumflexE4-S from E1circumflexE4 in vivo, E2circumflexE4-L could be formally identified as a 23 kDa protein in raft cultures in which the corresponding transcript was also found, and in a biopsy from a patient with cervical intraepithelial neoplasia stage I-II (CINI-II) associated with HPV-18, demonstrating the physiological relevance of E2circumflexE4 products.

  10. EA-1948: Revised Finding of No Significant Impact | Department of Energy

    Energy Savers [EERE]

    8: Revised Finding of No Significant Impact EA-1948: Revised Finding of No Significant Impact Gila-North Gila Transmission Line Rebuild and Upgrade Project, Yuma County, Arizona On March 21, 2014, DOE's Western Area Power Administration issued a finding of no significant impact (FONSI) for a proposal to rebuild and upgrade two parallel 4.8-mile transmission lines between the Gila and North Gila Substations, to expand its right-of-way, and to grant Arizona Power Service Company the right to cross

  11. V-057: eXtplorer "ext_find_user()" Authentication Bypass Vulnerability |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy 57: eXtplorer "ext_find_user()" Authentication Bypass Vulnerability V-057: eXtplorer "ext_find_user()" Authentication Bypass Vulnerability December 28, 2012 - 6:00am Addthis December 28 2012 - 6:00am PROBLEM: eXtplorer "ext_find_user()" Authentication Bypass Vulnerability PLATFORM: eXtplorer 2.x ABSTRACT: A vulnerability has been reported in eXtplorer, which can be exploited by malicious people to bypass certain security restrictions.

  12. V-057: eXtplorer "ext_find_user()" Authentication Bypass Vulnerability |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy 7: eXtplorer "ext_find_user()" Authentication Bypass Vulnerability V-057: eXtplorer "ext_find_user()" Authentication Bypass Vulnerability December 28, 2012 - 6:00am Addthis PROBLEM: eXtplorer "ext_find_user()" Authentication Bypass Vulnerability PLATFORM: eXtplorer 2.x ABSTRACT: A vulnerability has been reported in eXtplorer, which can be exploited by malicious people to bypass certain security restrictions. REFERENCE LINKS: Secunia Advisory

  13. EERE 2014 Wind Technologies Market Report Finds Wind Power at Record Low

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Prices | Department of Energy 2014 Wind Technologies Market Report Finds Wind Power at Record Low Prices EERE 2014 Wind Technologies Market Report Finds Wind Power at Record Low Prices August 10, 2015 - 11:00am Addthis EERE 2014 Wind Technologies Market Report Finds Wind Power at Record Low Prices David Friedman Principal Deputy Assistant Secretary for Energy Efficiency and Renewable Energy 2014 proved to be a strong year for the wind industry as the United States remained a global leader in

  14. EVMS Training Snippet: 1.8 DOE Common EVMS Findings | Department of Energy

    Office of Environmental Management (EM)

    8 DOE Common EVMS Findings EVMS Training Snippet: 1.8 DOE Common EVMS Findings This EVMS Training Snippet, sponsored by the Office of Project Management (PM) discusses the common surveillance findings identified during DOE EVMS Reviews. Link to Video Presentation | Prior Snippet (1.7) | Next Snippet (2.1) | Return to Index PDF icon Slides Only PDF icon Slides with Notes More Documents & Publications EVMS Training Snippet: 1.3 EVMS Stage 1 Surveillance EVMS Training Snippet: 4.3 Management

  15. Finding of No Significant Impact for the Storage of Tritium-Producing...

    Broader source: Energy.gov (indexed) [DOE]

    for the Storage of Tritium-Producing Burnable Absorber Rods in K-Area Transfer Bay at the Savannah River Site Agency: U.S. Department of Energy (DOE) Action: Finding of No...

  16. A difficult search, why Basin and Range systems are hard to find...

    Open Energy Info (EERE)

    hard to find Authors M.C. Richards and D.D. Blackwell Published Geothermal Resources Council Bulletin, 2002 DOI Not Provided Check for DOI availability: http:crossref.org...

  17. Tour of Zero: How you can help customers find better homes at...

    Broader source: Energy.gov (indexed) [DOE]

    Description: Are you interested in helping homebuyers find a better home at a lower cost? The DOE Tour of Zero provides the opportunity for visitors to take a virtual tour of...

  18. Tour of Zero: How you can Help Customers Find Better Homes at...

    Broader source: Energy.gov (indexed) [DOE]

    Description: Are you interested in helping homebuyers find a better home at a lower cost? The DOE Tour of Zero provides the opportunity for visitors to take a virtual tour of...

  19. Nudged-elastic band method with two climbing images: Finding transition states in complex energy landscapes

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Zarkevich, Nikolai A.; Johnson, Duane D.

    2015-01-09

    The nudged-elastic band (NEB) method is modified with concomitant two climbing images (C2-NEB) to find a transition state (TS) in complex energy landscapes, such as those with a serpentine minimal energy path (MEP). If a single climbing image (C1-NEB) successfully finds the TS, then C2-NEB finds it too. Improved stability of C2-NEB makes it suitable for more complex cases, where C1-NEB misses the TS because the MEP and NEB directions near the saddle point are different. Generally, C2-NEB not only finds the TS, but guarantees, by construction, that the climbing images approach it from the opposite sides along the MEP.more » In addition, C2-NEB provides an accuracy estimate from the three images: the highest-energy one and its climbing neighbors. C2-NEB is suitable for fixed-cell NEB and the generalized solid-state NEB.« less

  20. DOE ZERH Webinar: Tour of Zero, Helping Customers Find Better Homes at Lower Costs

    Broader source: Energy.gov [DOE]

    Are you interested in helping homebuyers find a better home at a lower cost? The DOE Tour of Zero provides the opportunity for visitors to take a virtual tour of high-performance Zero Energy Ready...

  1. EI2 Insulation Helps Anxious Pooch Find Calm in the Storm | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    I find that the house retains heat longer and is warmer all over, and that is great. But the aspect of having a dog with peace of mind during the times the wind picks up was worth ...

  2. Researchers find 3-D printed parts to provide low-cost, custom...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Researchers find 3-D printed parts to provide low-cost, custom alternatives for laboratory equipment By Raphael Rosen February 26, 2015 Tweet Widget Google Plus One Share on...

  3. EA-1861: DOE Notice of Availability of the Finding of No Significant...

    Broader source: Energy.gov (indexed) [DOE]

    Frito Lay Biomass Boiler Project, Beloit, Wisconsin The U.S. Department of Energy (DOE) has issued a Finding of No Significant Impact (FONSI) based on completion of an...

  4. Where might I find simplified Data on Capital, O&M, and fuel...

    Open Energy Info (EERE)

    Where might I find simplified Data on Capital, O&M, and fuel costs? Home I teach math to high school seniors. My students are studying energy in all their classes. I have just...

  5. Web Portal Makes Finding Ways to Drive Green Even Easier - News...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Web Portal Makes Finding Ways to Drive Green Even Easier From vehicle searches to interactive fuel maps, this Web page delivers October 13, 2009 The U.S. Department of Energy's ...

  6. Where can I find APIs and other resources for developers competing...

    Open Energy Info (EERE)

    Where can I find APIs and other resources for developers competing at Cleanweb hackathons? Home > Groups > Developer Submitted by Rmckeel on 25 June, 2012 - 07:23 1 answer Points:...

  7. In re Kent Pond, No MLP-03-10, Findings of Fact, Conclusions...

    Open Energy Info (EERE)

    re Kent Pond, No MLP-03-10, Findings of Fact, Conclusions of Law, and Order at 8, 13 (Vt. Water Res. Bd. May, 2004) Jump to: navigation, search OpenEI Reference LibraryAdd to...

  8. Estrogen induced concentration dependent differential gene expression in human breast cancer (MCF7) cells: Role of transcription factors

    SciTech Connect (OSTI)

    Chandrasekharan, Sabarinath; Kandasamy, Krishna Kumar; Dayalan, Pavithra; Ramamurthy, Viraragavan

    2013-08-02

    Highlights: Estradiol (E2) at low dose induced cell proliferation in breast cancer cells. E2 at high concentration induced cell stress in breast cancer cells. Estrogen receptor physically interacts only with a few transcription factors. Differential expression of genes with Oct-1 binding sites increased under stress. Transcription factor binding sites showed distinct spatial distribution on genes. -- Abstract: Background: Breast cancer cells respond to estrogen in a concentration dependent fashion, resulting in proliferation or apoptosis. The mechanism of this concentration dependent differential outcome is not well understood yet. Methodology: Meta-analysis of the expression data of MCF7 cells treated with low (1 nM) or high (100 nM) dose of estradiol (E2) was performed. We identified genes differentially expressed at the low or the high dose, and examined the nature of regulatory elements in the vicinity of these genes. Specifically, we looked for the difference in the presence, abundance and spatial distribution of binding sites for estrogen receptor (ER) and selected transcription factors (TFs) in the genomic region up to 25 kb upstream and downstream from the transcription start site (TSS) of these genes. Results: It was observed that at high dose E2 induced the expression of stress responsive genes, while at low dose, genes involved in cell cycle were induced. We found that the occurrence of transcription factor binding regions (TFBRs) for certain factors such as Sp1 and SREBP1 were higher on regulatory regions of genes expressed at low dose. At high concentration of E2, genes with a higher frequency of Oct-1 binding regions were predominantly involved. In addition, there were differences in the spatial distribution pattern of the TFBRs in the genomic regions among the two sets of genes. Discussion: E2 induced predominantly proliferative/metabolic response at low concentrations; but at high concentration, stressrescue responses were induced. At high E2 concentration, classical genomic pathway involving ER binding to the regulatory regions was reduced, and alternate or indirect activation of genes through Oct-1 became more prominent.

  9. EA-1611-S1: Finding of No Significant Impact | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    Colorado Highlands Wind Project, Logan County, Colorado DOE's Western Area Power Administration issued a Finding of No Significant Impact for the proposed expansion of Colorado Highlands Wind Project, which interconnects to Western's transmission system. PDF icon EA-1611-S1-FONSI-2014.pdf More Documents & Publications EA-1611-S1: Final Supplemental Environmental Assessment EA-1611: Finding of No Significant Impact EA-1611-S1: Draft Supplemental

  10. Scientists at ALS Find New Path to More Efficient Organic Solar Cells

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Scientists at ALS Find New Path to More Efficient Organic Solar Cells Scientists at ALS Find New Path to More Efficient Organic Solar Cells Print Monday, 07 January 2013 00:00 Harald Ade, a physicist at North Carolina State University, led a study at the Advanced Light Source that revealed a second pathway to improved performances of polymer/organic solar cells. Whereas the first pathway demands crystals of ultrapure domains, the new pathway shows that impure domains if sufficiently small can

  11. 2012 Congestion Study Webinars to Present Preliminary Findings and Receive Input from Stakeholders

    Broader source: Energy.gov [DOE]

    The Department of Energy will host three webinars in August 2012 to present the preliminary findings of the 2012 National Electric Transmission Congestion Study and to receive input and suggestions from state officials, industry representatives, and other stakeholders. Two of the webinars will be designed to discuss with state officials the initial findings of the DOE 2012 congestion analysis. The third webinar will be for industry representatives and other interested parties, although stakeholders may dial into any of the three meetings.

  12. ORISE Resources: How to Find Reliable HIV/AIDS Treatment Information on the

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Internet ORISE resource created for NLM assists in finding and evaluating HIV/AIDS information on the Web Produced by the Oak Ridge Institute for Science and Education (ORISE) for the National Library of Medicine, this document identifies websites that provide the most accurate and up-to-date information about HIV/AIDS treatment and research. Consulting quality HIV/AIDS websites is an efficient, inexpensive, and powerful way to find current information. It includes information on clinical

  13. DOE/EA-1976 FINDING OF NO SIGNIFICANT IMPACT FOR PROPOSED CNG PROJECT REGARDING

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    DOE/EA-1976 FINDING OF NO SIGNIFICANT IMPACT FOR PROPOSED CNG PROJECT REGARDING EMERA CNG, LLC APPLICATION SEEKING DEPARTMENT OF ENERGY AUTHORIZATION TO EXPORT COMPRESSED NATURAL GAS TO NON-FREE TRADE AGREEMENT NATIONS AGENCY: U.S. Department of Energy, Office of Fossil Energy ACTION: Finding of No Significant Impact SUMMARY: The U.S. Department of Energy (DOE) prepared an environmental assessment (EA) to evaluate the potential environmental impacts associated with the construction and operation

  14. Finding Hidden Oil and Gas Reserves | U.S. DOE Office of Science (SC)

    Office of Science (SC) Website

    Finding Hidden Oil and Gas Reserves Basic Energy Sciences (BES) BES Home About Research Facilities Science Highlights Benefits of BES Funding Opportunities Basic Energy Sciences Advisory Committee (BESAC) Community Resources Contact Information Basic Energy Sciences U.S. Department of Energy SC-22/Germantown Building 1000 Independence Ave., SW Washington, DC 20585 P: (301) 903-3081 F: (301) 903-6594 E: Email Us More Information » 10.01.12 Finding Hidden Oil and Gas Reserves Supercomputers +

  15. NREL Report Finds Similar Value in Two CSP Technologies - News Releases |

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    NREL Report Finds Similar Value in Two CSP Technologies Findings demonstrate increasing importance for systems with thermal energy storage February 11, 2014 Parabolic troughs and dry-cooled towers deliver similar value for concentrating solar power (CSP) plants, despite different solar profiles, a new report by the Energy Department's National Renewable Energy Laboratory has found. The report, "Estimating the Performance and Economic Value of Multiple Concentrating Solar Power

  16. DOE/EA-1905 FINDING OF NO SIGNIFICANT IMPACT DEPARTMENT OF ENERGY FUNDING FOR

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    905 FINDING OF NO SIGNIFICANT IMPACT DEPARTMENT OF ENERGY FUNDING FOR CITY OF CARLSBAD, NM DOUBLE EAGLE WATER SYSTEM AGENCY: U.S. Department of Energy Carlsbad Field Office (DOE CBFO) ACTION: Finding of No Significant Impact (FONSI) SUMMARY: The DOE CBFO is adopting an Environmental Assessment (EA) prepared by the U.S. Department of Interior Bureau of Land Management (BLM) Carlsbad Field Office for the expansion and upgrade of the City of Carlsbad's Double Eagle Water System. The City of

  17. EMGeo: Risk Minimizing Software for Finding Offshore Fossil Fuels by Fluid

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Identification - Energy Innovation Portal Energy Analysis Energy Analysis Find More Like This Return to Search EMGeo: Risk Minimizing Software for Finding Offshore Fossil Fuels by Fluid Identification Lawrence Berkeley National Laboratory Contact LBL About This Technology Plots of electrical conductivity over the Troll Field in the North Sea produced by analyzing 3D electromagnetic field data. Plots of electrical conductivity over the Troll Field in the North Sea produced by analyzing 3D

  18. Fernald Environmental Management Project Director's Final Findings & Orders, June 6, 1996

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    . - ~ June 6, 1996 Re: Director's Final Findings & Orders U.S. Department of Energy Cincinnati, Ohio U.S. EPA ID No: OH6890008976 Mr. Phil Hammric U.S. Department of Energy Femald Environmental Management Project P.O. Box 389705 Cincinnati, Ohio 45239 Mr. John Bradbume Femald Environmental Restoration Corporation P.0. Box 538704 Cincinnati, Ohio 45253-8704 Dear Mr. Hammric and Mr. Bradbume: Transmitted herewith are Final Findings and Orders of the Director concrrning the matter indicated

  19. Energy Dept. Report Finds Major Potential to Grow Clean, Sustainable U.S.

    Office of Environmental Management (EM)

    Hydropower | Department of Energy Report Finds Major Potential to Grow Clean, Sustainable U.S. Hydropower Energy Dept. Report Finds Major Potential to Grow Clean, Sustainable U.S. Hydropower April 29, 2014 - 9:15am Addthis News Media Contact (202) 586-4940 WASHINGTON - Building on the Obama Administration's commitment to an all-of-the-above energy strategy, the Energy Department and its Oak Ridge National Laboratory released a renewable energy resource assessment today detailing the

  20. FINDING OF NO SIGNIFICANT IMPACT FOR SABINE PASS LIQUEFACTION EXPANSION PROJECT REGARDING

    Office of Environmental Management (EM)

    FINDING OF NO SIGNIFICANT IMPACT FOR SABINE PASS LIQUEFACTION EXPANSION PROJECT REGARDING SABINE PASS LIQUEFACTION, LLC, APPLICATIONS SEEKING DEPARTMENT OF ENERGY AUTHORIZATION TO EXPORT LIQUEFIED NATURAL GAS FROM SABINE PASS LNG TERMINAL TO NON-FREE TRADE AGREEMENT NATIONS AGENCY: U.S. Department of Energy, Office of Fossil Energy ACTION: Finding of No Significant Impact SUMMARY: Pursuant to section 1501.6 of the regulations of the Council on Environmental Quality (CEQ), 40 CFR 1501.6, the U.S.

  1. Innovative Idaho Site Crews Find Ways to Make Waste Retrieval Safer, More

    Office of Environmental Management (EM)

    Efficient | Department of Energy Innovative Idaho Site Crews Find Ways to Make Waste Retrieval Safer, More Efficient Innovative Idaho Site Crews Find Ways to Make Waste Retrieval Safer, More Efficient June 30, 2015 - 12:00pm Addthis Advanced Mixed Waste Treatment Project retrieval crews safely remove the final box from Pad 1, Cell 2 located in the Transuranic Storage Area Retrieval Enclosure. On the left, the original box was degraded and could not be retrieved intact, so crews removed its

  2. Find Energy Incentives Quicker and Easier with DSIRE Open Data and Website

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    | Department of Energy Incentives Quicker and Easier with DSIRE Open Data and Website Find Energy Incentives Quicker and Easier with DSIRE Open Data and Website March 3, 2015 - 4:16pm Addthis The updated Database of State Incentives for Renewables & Efficiency helps homeowners and businesses find incentive programs that can reduce or defray installation or purchase costs of technologies like photovoltaic systems. | Photo by Dennis Schroeder, National Renewable Energy Laboratory The

  3. Small Catalyst Finding Could Lead to Big Breakthrough for Fuel Cell

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Deployment | Department of Energy Catalyst Finding Could Lead to Big Breakthrough for Fuel Cell Deployment Small Catalyst Finding Could Lead to Big Breakthrough for Fuel Cell Deployment April 29, 2014 - 3:02pm Addthis Researchers from the Energy Department's Berkeley and Argonne National Labs have developed a new class of fuel cell catalysts that uses roughly 85 percent less platinum and has more than 30 times the catalytic activity than conventional catalysts. The graphic above highlights

  4. Study Finds DOE-Funded Research in Energy Storage Provides a Vital

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Foundation for Success of Today's Hybrid and Electric Vehicles | Department of Energy Study Finds DOE-Funded Research in Energy Storage Provides a Vital Foundation for Success of Today's Hybrid and Electric Vehicles Study Finds DOE-Funded Research in Energy Storage Provides a Vital Foundation for Success of Today's Hybrid and Electric Vehicles This report discusses a study that found that U.S. Department of Energy-funded research in energy storage provides a vital foundation for the success

  5. DOE/EA-1976 FINDING OF NO SIGNIFICANT IMPACT FOR PROPOSED CNG PROJECT REGARDING

    Office of Environmental Management (EM)

    DOE/EA-1976 FINDING OF NO SIGNIFICANT IMPACT FOR PROPOSED CNG PROJECT REGARDING EMERA CNG, LLC APPLICATION SEEKING DEPARTMENT OF ENERGY AUTHORIZATION TO EXPORT COMPRESSED NATURAL GAS TO NON-FREE TRADE AGREEMENT NATIONS AGENCY: U.S. Department of Energy, Office of Fossil Energy ACTION: Finding of No Significant Impact SUMMARY: The U.S. Department of Energy (DOE) prepared an environmental assessment (EA) to evaluate the potential environmental impacts associated with the construction and operation

  6. EA-1339: Finding of No Significant Impact (Amended) | Department of Energy

    Office of Environmental Management (EM)

    Finding of No Significant Impact (Amended) EA-1339: Finding of No Significant Impact (Amended) Waste Disposition Activities at Paducah Site, Paducah, Kentucky The U.S. Department of Energy has completed an environmental assessment addendum (DOE/EA-1339-A), which is incorporated herein by reference, for proposed disposition of 17,600 m3 of waste from the Paducah Site in Paducah, Kentucky. It is anticipated that most of the waste would be transported for disposal at various locations in the United

  7. EA-1440-S1: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    Finding of No Significant Impact EA-1440-S1: Finding of No Significant Impact National Renewable Energy Laboratory's South Table Mountain Complex (Golden, Colorado) (see also DOE/EA-1440-S1 / DOE/EA-1609; DOE/EA-1440-S2 / DOE/EA-1738) In accordance with the Department of Energy National Environmental Policy Act (NEPA) implementing regulations, DOE evaluated the potential environmental impacts that would result from three actions at the National Renewable Energy Laboratory's (NREL) South Table

  8. EA-1605: Revised Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    Revised Finding of No Significant Impact EA-1605: Revised Finding of No Significant Impact The Department of Energy prepared an environmental assessment (EA) (DOE/EA-1605) to analyze the potential environmental impacts of the proposed construction and operation of new biomass cogeneration and heating facilities located at the Savannah River Site (SRS). A FONSI was issued in August 2008. In this revised FONSI, DOE proposes to design an construct a new heating plant to be located adjacent to the

  9. EA-1774-S1: Final Environmental Assessment and Finding of No Significant

    Office of Environmental Management (EM)

    Impact | Department of Energy 4-S1: Final Environmental Assessment and Finding of No Significant Impact EA-1774-S1: Final Environmental Assessment and Finding of No Significant Impact Energy Conservation Program: Energy Conservation Standards for Direct Heating Equipment (November 2011) For more information, contact: Mr. Mohammed Khan U.S. Department of Energy Office of Energy Efficiency and Renewable Energy Building Technologies Program, EE-2J 1000 Independence Avenue, SW., Washington, DC

  10. EA-1792-S1: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    Finding of No Significant Impact EA-1792-S1: Finding of No Significant Impact The U.S. Department of Energy (DOE) has completed a Supplemental Environmental Assessment (Supplemental EA)(DOE/EA-1792-S1) for the University of Maine's (UMaine) Deepwater Offshore Floating Wind Turbine Testing and Demonstration Project - Castine. Based on the analysis in the Supplemental EA, DOE has determined that the decision to authorize the expenditure of Congressionally directed federal funding by UMaine to

  11. EA-1849-S1: Finding of No Significant Impact | Department of Energy

    Office of Environmental Management (EM)

    -S1: Finding of No Significant Impact EA-1849-S1: Finding of No Significant Impact Phase II Facility - Ormat Tuscarora Geothermal Power Plant in Tuscarora, Nevada On the basis of the Final SEA, DOE has determined that issuing a disbursement from Federal loan guaranteed funding of to John Hancock Financial Services for Ormat to construct the Phase II facility at Tuscarora would not have a significant effect on the human environment. The preparation of an environmental impact statement is

  12. EM Finds Success with Fixed-Priced Hybrid Contract Approach Benefitting

    Office of Environmental Management (EM)

    Taxpayers | Department of Energy Finds Success with Fixed-Priced Hybrid Contract Approach Benefitting Taxpayers EM Finds Success with Fixed-Priced Hybrid Contract Approach Benefitting Taxpayers January 29, 2014 - 12:00pm Addthis A registered nurse with EM contractor HPM Corporation checks a patient's vital signs at the Hanford Occupational Medical Services Clinic. WASHINGTON, D.C. - EM plans to complete more fixed-priced procurements in the future, and some may involve a new combination of

  13. AAV-mediated delivery of the transcription factor XBP1s into the striatum reduces mutant Huntingtin aggregation in a mouse model of Huntington's disease

    SciTech Connect (OSTI)

    Zuleta, Amparo [Biomedical Neuroscience Institute, Faculty of Medicine, University of Chile, Santiago (Chile) [Biomedical Neuroscience Institute, Faculty of Medicine, University of Chile, Santiago (Chile); Center for Molecular Studies of the Cell, Institute of Biomedical Sciences, University of Chile, Santiago (Chile); Vidal, Rene L. [Biomedical Neuroscience Institute, Faculty of Medicine, University of Chile, Santiago (Chile) [Biomedical Neuroscience Institute, Faculty of Medicine, University of Chile, Santiago (Chile); Neurounion Biomedical Foundation, Santiago (Chile); Armentano, Donna; Parsons, Geoffrey [Department of Molecular Biology, Genzyme Corporation, 49 New York Avenue, Framingham, MA 01701 (United States)] [Department of Molecular Biology, Genzyme Corporation, 49 New York Avenue, Framingham, MA 01701 (United States); Hetz, Claudio, E-mail: chetz@med.uchile.cl [Biomedical Neuroscience Institute, Faculty of Medicine, University of Chile, Santiago (Chile) [Biomedical Neuroscience Institute, Faculty of Medicine, University of Chile, Santiago (Chile); Center for Molecular Studies of the Cell, Institute of Biomedical Sciences, University of Chile, Santiago (Chile); Department of Immunology and Infectious Diseases, Harvard School of Public Health, 651 Huntington Av., Boston, MA 02446 (United States); Neurounion Biomedical Foundation, Santiago (Chile)

    2012-04-13

    Highlights: Black-Right-Pointing-Pointer The contribution of ER stress to HD has not been directly addressed. Black-Right-Pointing-Pointer Expression of XBP1s using AAVs decreases Huntingtin aggregation in vivo. Black-Right-Pointing-Pointer We describe a new in vivo model of HD based on the expression of a large fragment of mHtt-RFP. -- Abstract: Huntington's disease (HD) is caused by mutations that expand a polyglutamine region in the amino-terminal domain of Huntingtin (Htt), leading to the accumulation of intracellular inclusions and progressive neurodegeneration. Recent reports indicate the engagement of endoplasmic reticulum (ER) stress responses in human HD post mortem samples and animal models of the disease. Adaptation to ER stress is mediated by the activation of the unfolded protein response (UPR), an integrated signal transduction pathway that attenuates protein folding stress by controlling the expression of distinct transcription factors including X-Box binding protein 1 (XBP1). Here we targeted the expression of XBP1 on a novel viral-based model of HD. We delivered an active form of XBP1 locally into the striatum of adult mice using adeno-associated vectors (AAVs) and co-expressed this factor with a large fragment of mutant Htt as a fusion protein with RFP (Htt588{sup Q95}-mRFP) to directly visualize the accumulation of Htt inclusions in the brain. Using this approach, we observed a significant reduction in the accumulation of Htt588{sup Q95}-mRFP intracellular inclusion when XBP1 was co-expressed in the striatum. These results contrast with recent findings indicating a protective effect of XBP1 deficiency in neurodegeneration using knockout mice, and suggest a potential use of gene therapy strategies to manipulate the UPR in the context of HD.

  14. An evaluation of accounting-based finding costs as efficiency measures for oil and gas exploration

    SciTech Connect (OSTI)

    Boynton, C.E. IV; Boone, J.P.

    1994-08-01

    The authors have operationalized firm-specific exploration efficiency as the difference between a firm-specific intercept estimated in a fixed-effects panel data Cobb-Douglas production frontier model and the maximum firm-specific intercept estimated in that model. The production model was estimated during two different time periods, 1982--1985 and 1989--1992, allowing efficiency to vary intertemporally. This efficiency estimate served as a benchmark against which they compared various measures of inverse finding costs. They assumed that the degree of association with an efficiency benchmark is an important attribute of any finding cost measure and that, further, the degree of association may be used as a metric for choosing between alternative finding cost measures. Accordingly, they evaluated the cross-sectional statistical association between estimated efficiency and alternative inverse finding cost measures. They discovered that the inverse finding cost measure that exhibited the strongest association with efficiency during the two time periods was a three-year moving-average finding cost which included exploration plus development expenditures as costs and reserve extensions and additions plus revisions as the units added.

  15. Uncovering Transcriptional Circuits in Arabidoposis by Functional Genomics (2013 DOE JGI Genomics of Energy and Environment 8th Annual User Meeting)

    SciTech Connect (OSTI)

    Pruneda-Paz, Jose [UC San Diego

    2013-03-01

    Jose Pruneda-Paz of UC San Diego on "Uncovering Transcriptional Circuits in Arabidopsis by Functional Genomics" at the 8th Annual Genomics of Energy & Environment Meeting in Walnut Creek, Calif.

  16. Transcript of See Action and Technical Assistance Program Webcast: Energy Audit and Retro-Commissioning Policies for Public and Commercial Buildings

    Broader source: Energy.gov [DOE]

    U.S. Department of Energy Office (DOE) of Energy Efficiency and Renewable Energy (EERE) transcript of See Action and Technical Assistance Program Webcast: Energy Audit and Retro-Commissioning Policies for Public and Commercial Buildings.

  17. Modeling the effects of vorinostat in vivo reveals both transient and delayed HIV transcriptional activation and minimal killing of latently infected cells

    SciTech Connect (OSTI)

    Ke, Ruian; Lewin, Sharon R.; Elliott, Julian H.; Perelson, Alan S.; Chakraborty, Arup K.

    2015-10-23

    Recent efforts to cure human immunodeficiency virus type-1 (HIV-1) infection have focused on developing latency reversing agents as a first step to eradicate the latent reservoir. The histone deacetylase inhibitor, vorinostat, has been shown to activate HIV RNA transcription in CD4+ T-cells and alter host cell gene transcription in HIV-infected individuals on antiretroviral therapy. In order to understand how latently infected cells respond dynamically to vorinostat treatment and determine the impact of vorinostat on reservoir size in vivo, we have constructed viral dynamic models of latency that incorporate vorinostat treatment. We fitted these models to data collected from a recent clinical trial in which vorinostat was administered daily for 14 days to HIV-infected individuals on suppressive ART. The results show that HIV transcription is increased transiently during the first few hours or days of treatment and that there is a delay before a sustained increase of HIV transcription, whose duration varies among study participants and may depend on the long term impact of vorinostat on host gene expression. Parameter estimation suggests that in latently infected cells, HIV transcription induced by vorinostat occurs at lower levels than in productively infected cells. Lastly, the estimated loss rate of transcriptionally induced cells remains close to baseline in most study participants, suggesting vorinostat treatment does not induce latently infected cell killing and thus reduce the latent reservoir in vivo.

  18. Modeling the effects of vorinostat in vivo reveals both transient and delayed HIV transcriptional activation and minimal killing of latently infected cells

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Ke, Ruian; Lewin, Sharon R.; Elliott, Julian H.; Perelson, Alan S.; Chakraborty, Arup K.

    2015-10-23

    Recent efforts to cure human immunodeficiency virus type-1 (HIV-1) infection have focused on developing latency reversing agents as a first step to eradicate the latent reservoir. The histone deacetylase inhibitor, vorinostat, has been shown to activate HIV RNA transcription in CD4+ T-cells and alter host cell gene transcription in HIV-infected individuals on antiretroviral therapy. In order to understand how latently infected cells respond dynamically to vorinostat treatment and determine the impact of vorinostat on reservoir size in vivo, we have constructed viral dynamic models of latency that incorporate vorinostat treatment. We fitted these models to data collected from a recentmore » clinical trial in which vorinostat was administered daily for 14 days to HIV-infected individuals on suppressive ART. The results show that HIV transcription is increased transiently during the first few hours or days of treatment and that there is a delay before a sustained increase of HIV transcription, whose duration varies among study participants and may depend on the long term impact of vorinostat on host gene expression. Parameter estimation suggests that in latently infected cells, HIV transcription induced by vorinostat occurs at lower levels than in productively infected cells. Lastly, the estimated loss rate of transcriptionally induced cells remains close to baseline in most study participants, suggesting vorinostat treatment does not induce latently infected cell killing and thus reduce the latent reservoir in vivo.« less

  19. Spent nuclear fuel project, Cold Vacuum Drying Facility human factors engineering (HFE) analysis: Results and findings

    SciTech Connect (OSTI)

    Garvin, L.J.

    1998-07-17

    This report presents the background, methodology, and findings of a human factors engineering (HFE) analysis performed in May, 1998, of the Spent Nuclear Fuels (SNF) Project Cold Vacuum Drying Facility (CVDF), to support its Preliminary Safety Analysis Report (PSAR), in responding to the requirements of Department of Energy (DOE) Order 5480.23 (DOE 1992a) and drafted to DOE-STD-3009-94 format. This HFE analysis focused on general environment, physical and computer workstations, and handling devices involved in or directly supporting the technical operations of the facility. This report makes no attempt to interpret or evaluate the safety significance of the HFE analysis findings. The HFE findings presented in this report, along with the results of the CVDF PSAR Chapter 3, Hazards and Accident Analyses, provide the technical basis for preparing the CVDF PSAR Chapter 13, Human Factors Engineering, including interpretation and disposition of findings. The findings presented in this report allow the PSAR Chapter 13 to fully respond to HFE requirements established in DOE Order 5480.23. DOE 5480.23, Nuclear Safety Analysis Reports, Section 8b(3)(n) and Attachment 1, Section-M, require that HFE be analyzed in the PSAR for the adequacy of the current design and planned construction for internal and external communications, operational aids, instrumentation and controls, environmental factors such as heat, light, and noise and that an assessment of human performance under abnormal and emergency conditions be performed (DOE 1992a).

  20. Local-global alignment for finding 3D similarities in protein structures

    DOE Patents [OSTI]

    Zemla, Adam T.

    2011-09-20

    A method of finding 3D similarities in protein structures of a first molecule and a second molecule. The method comprises providing preselected information regarding the first molecule and the second molecule. Comparing the first molecule and the second molecule using Longest Continuous Segments (LCS) analysis. Comparing the first molecule and the second molecule using Global Distance Test (GDT) analysis. Comparing the first molecule and the second molecule using Local Global Alignment Scoring function (LGA_S) analysis. Verifying constructed alignment and repeating the steps to find the regions of 3D similarities in protein structures.