Sample records for fatty acids derived

  1. Technetium radiodiagnostic fatty acids derived from bisamide bisthiol ligands

    DOE Patents [OSTI]

    Jones, Alun G. (Newton Centre, MA); Lister-James, John (Wellesley, MA); Davison, Alan (Needham, MA)

    1988-05-24T23:59:59.000Z

    A bisamide-bisthiol ligand containing fatty acid substituted thiol useful for producing Tc-labelled radiodiagnostic imaging agents is described. The ligand forms a complex with the radionuclide .sup.99m Tc suitable for administration as a radiopharmaceutical to obtain images of the heart for diagnosis of myocardial disfunction.

  2. Use of plant fatty acyl hydroxylases to produce hydroxylated fatty acids and derivatives in plants

    DOE Patents [OSTI]

    Somerville, Chris (Portola Valley, CA); van de Loo, Frank (Lexington, KY)

    2002-01-01T23:59:59.000Z

    The present invention relates to the identification of nucleic acid sequences and constructs, and methods related thereto, and the use of these sequences and constructs to produce genetically modified plants for the purpose of altering the composition of plant oils, waxes and related compounds.

  3. Use of plant fatty acyl hydroxylases to produce hydroxylated fatty acids and derivatives in plants

    DOE Patents [OSTI]

    Somerville, Chris (Portola Valley, CA); van de Loo, Frank (Lexington, KY)

    1998-01-01T23:59:59.000Z

    The present invention relates to the identification of nucleic acid sequences and constructs, and methods related thereto, and the use of these sequences and constructs to produce genetically modified plants for the purpose of altering the composition of plant oils, waxes and related compounds.

  4. Use of plant fatty acyl hydroxylases to produce hydroxylated fatty acids and derivatives in plants

    DOE Patents [OSTI]

    Somerville, Chris (Portola Valley, CA); van de Loo, Frank (Lexington, KY)

    1997-01-01T23:59:59.000Z

    The present invention relates to the identification of nucleic acid sequences and constructs, and methods related thereto, and the use of these sequences and constructs to produce genetically modified plants for the purpose of altering the composition of plant oils, waxes and related compounds.

  5. Mutant fatty acid desaturase

    DOE Patents [OSTI]

    Shanklin, John; Cahoon, Edgar B.

    2004-02-03T23:59:59.000Z

    The present invention relates to a method for producing mutants of a fatty acid desaturase having a substantially increased activity towards fatty acid substrates with chains containing fewer than 18 carbons relative to an unmutagenized precursor desaturase having an 18 carbon atom chain length substrate specificity. The method involves inducing one or more mutations in the nucleic acid sequence encoding the precursor desaturase, transforming the mutated sequence into an unsaturated fatty acid auxotroph cell such as MH13 E. coli, culturing the cells in the absence of supplemental unsaturated fatty acids, thereby selecting for recipient cells which have received and which express a mutant fatty acid desaturase with an elevated specificity for fatty acid substrates having chain lengths of less than 18 carbon atoms. A variety of mutants having 16 or fewer carbon atom chain length substrate specificities are produced by this method. Mutant desaturases produced by this method can be introduced via expression vectors into prokaryotic and eukaryotic cells and can also be used in the production of transgenic plants which may be used to produce specific fatty acid products.

  6. Fatty Acid Carcass Mapping

    E-Print Network [OSTI]

    Turk, Stacey N.

    2010-01-14T23:59:59.000Z

    calves as yearlings optimizes beef fatty acid composition. M.S. thesis, College Station: Texas A&M University. Chung, K.Y., Lunt, D.K., Choi, C.B., Chae, S.H., Rhoades, R.D., Adams, T.H., Booren, B., & Smith, S.B. (2006). Lipid characteristics... of subcutaneous adipose tissue and M. longissiumus thoracis of Angus and Wagyu steers fed to U.S. and Japanese endpoints. Meat Science, 73(3), 432-441. Chung, K.Y., Lunt, D.K., Kawachi, H., Yano, H., & Smith, S.B. (2005). Stearoyl coenzyme A desaturase...

  7. Polyunsaturated Fatty Acid Suppression of Hepatic Fatty Acid Synthase and S14 Gene Expression Does Not Require Peroxisome

    E-Print Network [OSTI]

    Omiecinski, Curtis

    of genes encoding fatty acid synthase, stearoyl-CoA desaturase, the S14 protein, and L-type pyruvate kinasePolyunsaturated Fatty Acid Suppression of Hepatic Fatty Acid Synthase and S14 Gene Expression Does, Maryland 20892 Dietary polyunsaturated fatty acids (PUFA) induce hepatic peroxisomal and microsomal fatty

  8. Systems Virology Identifies a Mitochondrial Fatty Acid Oxidation...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Fatty Acid Oxidation Enyzme, Dodecenoyl Coenzyme A Delta Isomerase, Required for Systems Virology Identifies a Mitochondrial Fatty Acid Oxidation Enyzme, Dodecenoyl Coenzyme...

  9. Production of hydroxylated fatty acids in genetically modified plants

    DOE Patents [OSTI]

    Somerville, Chris (Portola Valley, CA); Broun, Pierre (Burlingame, CA); van de Loo, Frank (Weston, AU); Boddupalli, Sekhar S. (Manchester, MI)

    2011-08-23T23:59:59.000Z

    This invention relates to plant fatty acyl hydroxylases. Methods to use conserved amino acid or nucleotide sequences to obtain plant fatty acyl hydroxylases are described. Also described is the use of cDNA clones encoding a plant hydroxylase to produce a family of hydroxylated fatty acids in transgenic plants. In addition, the use of genes encoding fatty acid hydroxylases or desaturases to alter the level of lipid fatty acid unsaturation in transgenic plants is described.

  10. Production of hydroxylated fatty acids in genetically modified plants

    DOE Patents [OSTI]

    Somerville, Chris; Broun, Pierre; van de Loo, Frank; Boddupalli, Sekhar S.

    2005-08-30T23:59:59.000Z

    This invention relates to plant fatty acyl hydroxylases. Methods to use conserved amino acid or nucleotide sequences to obtain plant fatty acyl hydroxylases are described. Also described is the use of cDNA clones encoding a plant hydroxylase to produce a family of hydroxylated fatty acids in transgenic plants. In addition, the use of genes encoding fatty acid hydroxylases or desaturases to alter the level of lipid fatty acid unsaturation in transgenic plants is described.

  11. Fatty acid synthase plays a role in cancer metabolism beyond providing fatty acids for phospholipid synthesis or sustaining elevations in glycolytic activity

    SciTech Connect (OSTI)

    Hopperton, Kathryn E., E-mail: kathryn.hopperton@mail.utoronto.ca [Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, Toronto, ON, Canada M5S 3E2 (Canada); Duncan, Robin E., E-mail: robin.duncan@uwaterloo.ca [Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, Toronto, ON, Canada M5S 3E2 (Canada); Bazinet, Richard P., E-mail: richard.bazinet@utoronto.ca [Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, Toronto, ON, Canada M5S 3E2 (Canada); Archer, Michael C., E-mail: m.archer@utoronto.ca [Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, Toronto, ON, Canada M5S 3E2 (Canada); Department of Medical Biophysics, Faculty of Medicine, University of Toronto, Toronto, ON, Canada M5S 3E2 (Canada)

    2014-01-15T23:59:59.000Z

    Fatty acid synthase is over-expressed in many cancers and its activity is required for cancer cell survival, but the role of endogenously synthesized fatty acids in cancer is unknown. It has been suggested that endogenous fatty acid synthesis is either needed to support the growth of rapidly dividing cells, or to maintain elevated glycolysis (the Warburg effect) that is characteristic of cancer cells. Here, we investigate both hypotheses. First, we compared utilization of fatty acids synthesized endogenously from {sup 14}C-labeled acetate to those supplied exogenously as {sup 14}C-labeled palmitate in the culture medium in human breast cancer (MCF-7 and MDA-MB-231) and untransformed breast epithelial cells (MCF-10A). We found that cancer cells do not produce fatty acids that are different from those derived from exogenous palmitate, that these fatty acids are esterified to the same lipid and phospholipid classes in the same proportions, and that their distribution within neutral lipids is not different from untransformed cells. These results suggest that endogenously synthesized fatty acids do not fulfill a specific function in cancer cells. Furthermore, we observed that cancer cells excrete endogenously synthesized fatty acids, suggesting that they are produced in excess of requirements. We next investigated whether lipogenic activity is involved in the maintenance of high glycolytic activity by culturing both cancer and non-transformed cells under anoxic conditions. Although anoxia increased glycolysis 2–3 fold, we observed no concomitant increase in lipogenesis. Our results indicate that breast cancer cells do not have a specific qualitative or quantitative requirement for endogenously synthesized fatty acids and that increased de novo lipogenesis is not required to sustain elevations in glycolytic activity induced by anoxia in these cells. - Highlights: • Fatty acid synthase (FASN) is over-expressed in cancer but its function is unknown. • We compare utilization of fatty acids produced by FASN to those derived exogenously. • Cancer cells do not have a specific requirement for fatty acids produced by FASN. • Fatty acids produced by FASN are in excess of cell requirements and are excreted. • Increased FASN activity is not required to sustain elevations in glycolysis.

  12. Production of hydroxylated fatty acids in genetically modified plants

    DOE Patents [OSTI]

    Somerville, Chris (Portola Valley, CA); Broun, Pierre (Burlingame, CA); van de Loo, Frank (Lexington, KY)

    2001-01-01T23:59:59.000Z

    This invention relates to plant fatty acyl hydroxylases. Methods to use conserved amino acid or nucleotide sequences to obtain plant fatty acyl hydroxylases are described. Also described is the use of cDNA clones encoding a plant hydroxylase to produce a family of hydroxylated fatty acids in transgenic plants.

  13. THYROID HORMONE REVERSES AGING-INDUCED MYOCARDIAL FATTY ACID...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    occurs during aging in humans and mice and may contribute to development of heart failure. Aging also impairs myocardial fatty acid oxidation, causing increased...

  14. Fatty Acid Biosynthesis Caught in the Act | Stanford Synchrotron...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    the enzymes involved in fatty acid biosynthesis serve as models for novel antimicrobials development and biofuels engineering. Figure 1. E. coli AcpP and crosslinking strategy. a,...

  15. Glucose oxidation in heart-type fatty acid binding protein null mice 

    E-Print Network [OSTI]

    Adhikari, Sean

    2006-10-30T23:59:59.000Z

    Heart-type fatty acid binding protein (H-FABP) is a major fatty acid binding factor in skeletal muscles. Genetic lack of H-FABP severely impairs the esterification and oxidation of exogenous fatty acids in soleus muscles ...

  16. Genotypic influences on fatty acid profiles and germinability of pecan pollen

    E-Print Network [OSTI]

    Nail, William R

    1996-01-01T23:59:59.000Z

    genotypes. Total saturated and unsaturated fatty acids influenced pollen germination to a greater degree than individual fatty acids. The effects of fatty acids on pollen germination of protandrous genotypes were opposite those on 'Stuart', the only...

  17. Human platelet aggregation and phospholipid fatty acid composition during omega-3 fatty acid enriched egg consumption: influence of nutrient intake and omega-3 fatty acid source

    E-Print Network [OSTI]

    Hatch, Sandra D

    1997-01-01T23:59:59.000Z

    The current study investigated the usefulness of omega-3 polyunsaturated fatty acid (n-3 PUFA) enriched eggs for duplicating health benefits of consuming fish, specifically decreasing platelet aggregation via changes in plasma phospholipid (PL...

  18. An insect assay for essential fatty acids 

    E-Print Network [OSTI]

    Kerur, Dundappa Ramachandrappa

    1956-01-01T23:59:59.000Z

    Ltc sn6 arachl@ontc acl6s 'es members sf' the essential fatty acid group. naut; defzolencp spmy&ms in the rat, associated cath e. fat &red Met, axes s) retardee@ growth, 'b) increased z YAM a%one o ' SMce She orz. g~l recognlM;on of zznolekc...'QPGP-, ' I T~ OP QQFg~V25 P8j. , X%4&4?C 4IQ? o e''e t ~ e jt 0 e s'o X o, s t tp ~ s o o o p e o * |p 0 o ~ t v o t s o ~ ~ o o o s", I" 'a "- "" K'1844X PCS, BCVlCV p e t's ?'o s e e tt p t e t p t a ~ t' o' o s o 's ~ s' 't o t' ~, ~ o'o a' A~t8ng58...

  19. The hydrogenation of dietary unsaturated fatty acids by the ruminant

    E-Print Network [OSTI]

    Reddy, H. G. Ramakrishna

    1955-01-01T23:59:59.000Z

    at the double bonds (9), for, if this had occurred the mean molecular weights would have been quite low. The difference in composition of the fatty acids of the rumen contents of goats l and Z, is obviously due to the samples having been under different...LIBRARY g 4 M COLLEGE Pf TEXIIG THE HYDROGENATION OF DIETARY UNSATURATED FATTY ACIDS SY THE RUMINANT A The sis H. G. RAMAKRISHNA REDDY Submitted to the Graduate School of the Agni cultural and Mechanical College of Texas in partial...

  20. Modification of the fatty acid composition of bovine tissues

    E-Print Network [OSTI]

    Chang, Joyce

    1990-01-01T23:59:59.000Z

    for the oxidation of the saturated fatty acid to produce a molecule of water and a cis double bond between the 9th and 10th carbons (Brenner, 19). Numerous studies have shown that the desaturase system is sensitive to a wide variety of extraneous factors (20, 21... the location of the fatty acids under ultraviolet light. The spots depicting oleate and stearate separated due to the double bond in the oleate which provided the stearate greater mobility. These spots were scraped and ran on the liquid scintillation...

  1. Enrichment of selected fatty acids in broiler tissues

    E-Print Network [OSTI]

    Yau, Jia-Chyi

    1990-01-01T23:59:59.000Z

    ENRICHMENT OF SELECTED FATTY ACIDS IN BROILER TISSUES A Thesis by JIA-CHYI YAU Submitted to the Office of Graduate Study of Texas A&M University in partial fullfillment of the requirements for the degree of MASTER OF SCIENCE August 1990... Major Subject: Food Science and Technology ENRICHMENT OF SELECTED FATTY ACIDS IN BROILER TISSUES A Thesis by JIA-CHYI YAU Approved as to style and content by A. R. Sams (Chair of Comittee) C. A. Bailey (Member) J. T Eeet n (M mber) R. Creg...

  2. Mutant fatty acid desaturase and methods for directed mutagenesis

    DOE Patents [OSTI]

    Shanklin, John (Shoreham, NY); Whittle, Edward J. (Greenport, NY)

    2008-01-29T23:59:59.000Z

    The present invention relates to methods for producing fatty acid desaturase mutants having a substantially increased activity towards substrates with fewer than 18 carbon atom chains relative to an unmutagenized precursor desaturase having an 18 carbon chain length specificity, the sequences encoding the desaturases and to the desaturases that are produced by the methods. The present invention further relates to a method for altering a function of a protein, including a fatty acid desaturase, through directed mutagenesis involving identifying candidate amino acid residues, producing a library of mutants of the protein by simultaneously randomizing all amino acid candidates, and selecting for mutants which exhibit the desired alteration of function. Candidate amino acids are identified by a combination of methods. Enzymatic, binding, structural and other functions of proteins can be altered by the method.

  3. aberrant fatty acid-binding: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The adipocyte fatty acid-binding protein aP2 regulates systemic glucose (more) Shum, Bennett Oh Vic 2007-01-01 2 Fatty acid-binding protein in bovine skeletal muscle Texas A&M...

  4. Radiolabeled dimethyl branched long chain fatty acid for heart imaging

    DOE Patents [OSTI]

    Knapp, Jr., Furn F. (Oak Ridge, TN); Goodman, Mark M. (Knoxville, TN); Kirsch, Gilbert (Woippy, FR)

    1988-08-16T23:59:59.000Z

    A radiolabeled long chain fatty acid for heart imaging that has dimethyl branching at one of the carbons of the chain which inhibits the extent to which oxidation can occur. The closer to the carboxyl the branching is positioned, the more limited the oxidation, thereby resulting in prolonged retention of the radiolabeled compound in the heart.

  5. Marketability of omega-3 fatty acid enriched shell eggs

    E-Print Network [OSTI]

    Marshall, Autumn Chester

    1994-01-01T23:59:59.000Z

    further demonstrated the negative consequences of this activation by finding that metmyoglobin interacts with hydrogen peroxide to initiate lipid oxidation in membranes (38). Such damage to membranes could be detrimental in vivo. Other free ionic... series of reactions of the carbon chains of unsaturated lipids with oxygen to form compounds of varying volatility. The initial step of lipid oxidation involves the removal of a hydrogen radical from the fatty acid carbon chain. The double bonds...

  6. An insect assay for essential fatty acids

    E-Print Network [OSTI]

    Kerur, Dundappa Ramachandrappa

    1956-01-01T23:59:59.000Z

    ~ " ' ?, ? '-' exyex wen4ji'. M@leahi@. th@4 tJie yieaenee:. . of douM. 'e:boccie st ths'6t'f en@ g(10' poa3;Mgna, couzL52Eg, -'gx QN. Mle le?Q~ Th's'b'loant9vkty'6eexeaaea. vhen the nueLbeg . of-. . double, honda '. gene'alon nay aoiuel3, @ 9nezsieas the eaaen~3. a... ~ ~ &&. 7 3; @. 8; '' &7 . " T9;& C 3. 2$ ~G . K . . x6. g 1. g. 5 g . G. x e::. e ', , g5 gx. '8, '; ', l. ( N ops imp@i ieveio ' coxx&dgetje amex'pence q? f~i j ' gcvnL~G@ I 'motIA ie 'abahvvet3@', . -Xejg. ex' QQgg6ecect 52$t. -'i~leic acid...

  7. Importance of exogenous saturated fatty acids during brain development and myelination in mice

    E-Print Network [OSTI]

    Boyer, Edmond

    part of the cell, in mitochondria and in microsomes (Bourre et al., 1977a). Radioactive saturated fatty. The labelled stearic acid taken up was partly metabolized in the brain either by elongation or by degradation and in situ resynthesis of fatty acids. The activity of oleic acid and mono-unsaturated chains was detectable

  8. Effects of Light and Temperature on Fatty Acid Production in Nannochloropsis Salina

    SciTech Connect (OSTI)

    Van Wagenen, Jonathan M.; Miller, Tyler W.; Hobbs, Samuel J.; Hook, Paul W.; Crowe, Braden J.; Huesemann, Michael H.

    2012-03-12T23:59:59.000Z

    Accurate prediction of algal biofuel yield will require empirical determination of physiological responses to the climate, particularly light and temperature. One strain of interest, Nannochloropsis salina, was subjected to ranges of light intensity (5-850 {mu}mol m{sup -2} s{sup -1}) and temperature (13-40 C); exponential growth rate, total fatty acids (TFA) and fatty acid composition were measured. The maximum acclimated growth rate was 1.3 day{sup -1} at 23 C and 250 {mu}mol m{sup -2} s{sup -1}. Fatty acids were detected by gas chromatography with flame ionization detection (GC-FID) after transesterification to corresponding fatty acid methyl esters (FAME). A sharp increase in TFA containing elevated palmitic acid (C16:0) and palmitoleic acid (C16:1) during exponential growth at high light was observed, indicating likely triacylglycerol accumulation due to photo-oxidative stress. Lower light resulted in increases in the relative abundance of unsaturated fatty acids; in thin cultures, increases were observed in palmitoleic and eicosapentaenoeic acids (C20:5{omega}3). As cultures aged and the effective light intensity per cell converged to very low levels, fatty acid profiles became more similar and there was a notable increase of oleic acid (C18:1{omega}9). The amount of unsaturated fatty acids was inversely proportional to temperature, demonstrating physiological adaptations to increase membrane fluidity. This data will improve prediction of fatty acid characteristics and yields relevant to biofuel production.

  9. The Total Fatty Acids and Other Ether-Soluable Constituents of Feedstuffs.

    E-Print Network [OSTI]

    Rather, J. B. (James Burness)

    1914-01-01T23:59:59.000Z

    586-914-10m TEXAS AGRICULTURAL EXPERIMENT STATION BULLETIN NO. 169 SEPTEMBER, 19I4 DIVISION OF CHEMISTRY The Total Fatty Acids and Other Ether-Soluble Constituents of Feedstuffs BY J. B. RATHER Assistant Chemist POSTOFFICE COLLEGE STATION............................................................................................................. 5 An Improved Method for the Determination of Total Fatty Acids and Other Constituents of Ether Extracts................................... 7 A New Method for the Extraction of Total Fatty Acids and Other Constituents of Feed Stuffs...

  10. A study of the distribution of fatty acids in the system: cottonseed oil-oleic acid-isopropanol-water

    E-Print Network [OSTI]

    Lamb, Frank E

    1948-01-01T23:59:59.000Z

    STUDY OF THE DISTHIBUTION OF FATTY ACIDS IN THE SYSTEM: COTTONSEED OIL - OLEIC ACID - ISOFHOPANOL - WATER A Thesis By Frank E. Lamb January 1948 A STUDY QF TNE DISTRIBUTION OF FATTY ACIDS IN TRE SYSTEM: COTTONSEED OII, - OIEIC ACID - ISOPROPANOL...A STDDY OF THI' DISTBIRliTION OF FATTY ACIDS IN THE SYSTEM: COTTONSFED OIL - OLEIC ACID - ISOPBOPANOL - YlATEB A Thesis Frank E. Lomb January 1948 Approval as to style and content recommended: Head ' t Te epartm nt of hem cal Engineer ng A...

  11. Impact of Omega-3 Polyunsaturated Fatty Acids on Bone Adaptations to Simulated Resistance Training 

    E-Print Network [OSTI]

    Camp, Kaleigh Ann

    2013-04-30T23:59:59.000Z

    Young and ovariectomized animals eating diets rich in omega-3 polyunsaturated fatty acids (n-3 PUFAs) exhibit enhanced bone formation and decrease bone loss, respectively. Eicosapentaenoic acid, an n-3 PUFA found in fish ...

  12. The Role of Pyruvate Dehydrogenase and Acetyl-Coenzyme A Synthetase in Fatty Acid

    E-Print Network [OSTI]

    Wurtele, Eve Syrkin

    The Role of Pyruvate Dehydrogenase and Acetyl-Coenzyme A Synthetase in Fatty Acid Synthesis of fatty acids and, through them, a range of important biomolecules. The source of acetyl pyruvate dehydrogenase. To determine the importance of these two enzymes in synthesizing acetyl-CoA during

  13. Studies on the isolation and properties of the highly unsaturated fatty acids from natural sources

    E-Print Network [OSTI]

    Abu-Nasr, Ahmed Mostafa

    1953-01-01T23:59:59.000Z

    Adrenal Lipids ? ??. 6m Rapid Concentration of Polyunsaturated Methyl and Ethyl Esters of Cod Liver Oil by Urea Complexes . o ? C. CONCENTRATION BY LOW TEMPERATURE CRYSTALLIZATION . . . . \\ IV. ISOLATION OF INDIVIDUAL HIGHLY UNSATURATED FATTY ACIDS... octadecatetraenoate by elution ? 0 ? . . . Ill b. Methyl arachidonate from beef testes fat by elution............................... . 115 5. Effect of Vacuum Distillation on the Polyunsaturated Fatty Acids ? ? ? ? . ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 117 6...

  14. Intestinal fatty acid digestion and energy utilization in lambs infused with different plant oils

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    Intestinal fatty acid digestion and energy utilization in lambs infused with different plant oils the effects of different plant oils infused into the abomasum on intestinal fatty acid digestion and energy oil (RO) with Cr-EDTA was continuously infused into the abomasum for 17 days of each period. The daily

  15. Fatty Acid Chemistry at the Oil-Water Interface: Self-Propelled Oil Droplets

    E-Print Network [OSTI]

    Ikegami, Takashi

    Fatty Acid Chemistry at the Oil-Water Interface: Self-Propelled Oil Droplets Martin M. Hanczyc transitions. Here we have explored the possibility that fatty acid systems also demonstrate movement. An oil solution. The oil droplets showed autonomous, sustained movement through the aqueous media. Internal

  16. Color and fatty acid profiles of Texas pecans

    E-Print Network [OSTI]

    Ratcliff, James R.

    1989-01-01T23:59:59.000Z

    Information on the kernel testa color and the fatty acid profiles for cultivars, selections, and native seedlings from four locations in Texas was provided. On the average, colorimeter 'b' to 'a' ratio was 1. 58; 'L' was 35. G6; 'a' was 9. 56; 'b' was 15. 13...b Shoshoni 1. 46b Kiowa 1. 37bc Burkett 1. 27cd 37. 92bc 31. 58f 29. 3(g 11. 63b 16. 83a 9. 45d 12. 86c 9. 87cd 12. 42c wichita iAIohawk Cherokee l. 16de 1. 16de 1. 01e ++ I 35. 58d 32. 64f 27. 82g 12. 97a t 14. 96b 12. 55ab I 14...

  17. Effect of Conjugated Linoleic Acid or Oleic Acid Addition on Fatty Acid Composition Profiles of Poultry Meat

    E-Print Network [OSTI]

    Shin, Dae Keun

    2011-08-08T23:59:59.000Z

    on the omega-6 fatty acid accumulation in broiler chicken breast and thigh meat. Eight broilers from each treatment were processed at 4 and 6 weeks of age, respectively. Regarding the diets containing five different fat sources, broiler chickens fed CLA...

  18. Polar lipid fatty acids, LPS-hydroxy fatty acids, and respiratory quinones of three Geobacter strains, and variation with electron acceptor

    SciTech Connect (OSTI)

    Hedrick, David B.; Peacock, Aaron; Lovley, Derek; Woodard, Trevor L.; Nevin, Kelly P.; Long, Philip E.; White, David C.

    2009-02-01T23:59:59.000Z

    The polar lipid fatty acids, lipopolysaccharide hydroxy-fatty acids, and respiratory quinones of Geobacter metallireducens str. GS-15, Geobacter sulfurreducens str. PCA, and Geobacter bemidjiensis str. Bem are reported. Also, the lipids of G. metallireducens were compared when grown with Fe3+ or nitrate as electron acceptors and G. sulfurreducens with Fe3+ or fumarate. In all experiments, the most abundant polar lipid fatty acids were 14:0, i15:0, 16:1*7c, 16:1*5c, and 16:0; lipopolysaccharide hydroxyfatty acids were dominated by 3oh16:0, 3oh14:0, 9oh16:0, and 10oh16:0; and menaquinone-8 was the most abundant respiratory quinone. Some variation in lipid proWles with strain were observed, but not with electron acceptor.

  19. Effect of dietary cysteine, methionine, and sterculic acid on fatty acid distribution in rat adipose tissue

    E-Print Network [OSTI]

    Brotze, Mary Frances

    1968-01-01T23:59:59.000Z

    . Statistical Anal sis The data were treated according to the analysis of variance for data with a single criterion of classifica- tion(24). Each of the ratios for the triglyceride frac- tion were analyzed as: Source oi Variation De rees of Freedom Total... ACIDS IN ADIPOSE TISSUE OF THE RAT B. Free Fatty Acid Fraction Group No. Sterculia f~oa ao 1 Methionine level in diet Cysteine level in diet 16/16:1 18/18:1 18/18:2 18:1/18:2 III IV VI VII VIII 0. 2 0. 2 0. 2 0. 2 low low high...

  20. Effect of dietary cysteine, methionine, and sterculic acid on fatty acid distribution in rat adipose tissue 

    E-Print Network [OSTI]

    Brotze, Mary Frances

    1968-01-01T23:59:59.000Z

    . Statistical Anal sis The data were treated according to the analysis of variance for data with a single criterion of classifica- tion(24). Each of the ratios for the triglyceride frac- tion were analyzed as: Source oi Variation De rees of Freedom Total... ACIDS IN ADIPOSE TISSUE OF THE RAT B. Free Fatty Acid Fraction Group No. Sterculia f~oa ao 1 Methionine level in diet Cysteine level in diet 16/16:1 18/18:1 18/18:2 18:1/18:2 III IV VI VII VIII 0. 2 0. 2 0. 2 0. 2 low low high...

  1. An integrated computational and experimental study for overproducing fatty acids in Escherichia coli

    E-Print Network [OSTI]

    Maranas, Costas

    for petroleum have stimulated sustainable ways to produce chemicals and biofuels. Specifically, fatty acids for the catalytic production of industrial chemicals and diesel- like biofuels. However, bio-production of fatty., 2008); (Steen et al., 2010); (Handke et al., 2011); (Liu et al., 2010a)). First generation biofuels

  2. A role for AMPK in the inhibition of glucose-6-phosphate dehydrogenase by polyunsaturated fatty acids

    SciTech Connect (OSTI)

    Kohan, Alison B.; Talukdar, Indrani; Walsh, Callee M. [Department of Biochemistry, West Virginia University, Morgantown, WV (United States)] [Department of Biochemistry, West Virginia University, Morgantown, WV (United States); Salati, Lisa M., E-mail: lsalati@hsc.wvu.edu [Department of Biochemistry, West Virginia University, Morgantown, WV (United States)

    2009-10-09T23:59:59.000Z

    Both polyunsaturated fatty acids and AMPK promote energy partitioning away from energy consuming processes, such as fatty acid synthesis, towards energy generating processes, such as {beta}-oxidation. In this report, we demonstrate that arachidonic acid activates AMPK in primary rat hepatocytes, and that this effect is p38 MAPK-dependent. Activation of AMPK mimics the inhibition by arachidonic acid of the insulin-mediated induction of G6PD. Similar to intracellular signaling by arachidonic acid, AMPK decreases insulin signal transduction, increasing Ser{sup 307} phosphorylation of IRS-1 and a subsequent decrease in AKT phosphorylation. Overexpression of dominant-negative AMPK abolishes the effect of arachidonic acid on G6PD expression. These data suggest a role for AMPK in the inhibition of G6PD by polyunsaturated fatty acids.

  3. C resonance assignment of the acyl carrier protein subunit of the Saccharomyces cerevisiae fatty acid synthase

    E-Print Network [OSTI]

    Wider, Gerhard

    of the Saccharomyces cerevisiae fatty acid synthase Daniel R. Perez Ã? Gerhard Wider Received: 13 January 2009 the structural changes upon apo- holo transformation in order to unveil the mechanism of binding of the growing

  4. Influence of Fatty Alcohols and Acids on the Clarity and Biota of Impounded Reservoirs

    E-Print Network [OSTI]

    Davis, W. B.; Reynolds, T. D.

    TR-18 1969 Influence of Fatty Alcohols and Acids on the Clarity and Biota of Impounded Reservoirs W.B. Davis T.D. Reynolds Texas Water Resources Institute Texas A&M University ...

  5. Imflammatory response in horses fed diets containing omega-6 and omega-3 fatty acids 

    E-Print Network [OSTI]

    Wilson, Kristopher Ray

    2003-01-01T23:59:59.000Z

    This study was conducted to characterize the inflammatory response of horses to chronic and acute exercise as influenced by dietary fat supplementation. Different ratios of omega 6 vs. omega 3 fatty acid supplementation ...

  6. Viscosity of plant oils as a function of temperature, fatty acid chain length, and unsaturation

    E-Print Network [OSTI]

    Neo, Tong Heng

    1988-01-01T23:59:59.000Z

    for the degree of MASTER OF SCIENCE December 1988 Major Subject: Agricultural Engineering VISCOSITY OF PLANT OILS AS A FUNCTION OF TEMPERATURE, FATTY ACID CHAIN LENGTH, AND UNSATURATION A Thesis by TONG HENG NEO Approved as to style and content by...: Vincent E. Sweat (Chairman of Committee) Ron L. Richter (Member) R. Engler (Member) Edward A. Hiler (Head of the Department) December 1988 ABSTRACT Viscosity of Plant Oils as a Function of Temperature, Fatty Acid Chain Length, and Unsaturation...

  7. Effects of dietary fiber and carcinogen on fatty acid binding protein expression in exfoliated colonocytes

    E-Print Network [OSTI]

    Clark, Amy Eunice

    1997-01-01T23:59:59.000Z

    EFFECTS OF DIETARY FIBER AND CARCINOGEN ON FATTY ACID BINDING PROTEIN EXPRESSION IN EXFOLIATED COLONOCYTES A Thesis by AMY EUNICE CLARK Submitted to the Office of Gmduate Studies of Texas A&M University in partial fulfillment...) Bryan H. Johnson (Head of Department) August 1997 Major Subject: Nutrition ABSTRACT Effects of Dietary Fiber and Carcinogen on Fatty Acid Binding Protein Expression in Exfoliated Colonocytes. (August 1997) Amy Eunice Clark, B. S. , Texas A...

  8. Modifying yolk fatty acid composition to improve the health quality of shell eggs

    E-Print Network [OSTI]

    Van Elswyk, Mary Elizabeth

    1990-01-01T23:59:59.000Z

    MODIFYING YOLK FATTY ACID COMPOSITION TO IMPROVE THE HEALTH QUALITY OF SHELL EGGS A Thesis by MARY ELIZABETH VAN ELSWYK Submitted to the Office of Graduate Studies of Texas A&M University in partial fulfillment of the requirements... for the degree of MASTER OF SCIENCE August 1990 Major Subject: Nutrition MODIFYING YOLK FATTY ACID COMPOSITION TO IMPROVE THE HEALTH QUALITY OF SHELL EGGS A Thesis by MARY ELIZABETH VAN ELSWYK Approved as to style and content by: Pamela S. Har s (Chair...

  9. Influence of dietary fat on the fatty acid composition of egg yolks and reproductive performance in the turkey

    E-Print Network [OSTI]

    Cornett, Bonita Manis

    1966-01-01T23:59:59.000Z

    of Soybean Oil and Neatsfoot Oil 36 Table 4 - Patty Acid Composition of Diets 37 Table 5 - Yolk Fatty Acids at One and Three Months of Dietary Treatment (Polar Praction) 38 Table 6 - Yolk Fatty Acids at One and Three Months of Dietary Treatment (Neutral... fatty acids. The following study was undertaken to evaluate (1) the effects of feeding turkey hens on a fat- free diet or diets containing un- saturated oils, on the subsequent fatty acid composition in the yolk, and (2) the effects of the various...

  10. n-3 Polyunsaturated Fatty Acids Alter Mouse CD4+ T Cell Activation by Modifying the Lipid Bilayer Properties 

    E-Print Network [OSTI]

    Hou, Tim Yu-Tien

    2014-12-05T23:59:59.000Z

    Epidemiological and clinical studies have shown that very long chain n-3 polyunsaturated fatty acids (n-3 PUFA) such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) possess anti-inflammatory properties. The ...

  11. Immunosuppressive dietary n-3 polyunsaturated fatty acids differentially modulate costimulatory regulation of murine CD4+ T-cell function 

    E-Print Network [OSTI]

    Ly, Lan H.

    2005-02-17T23:59:59.000Z

    Consumption of fish oils (FO) enriched with the n-3 polyunsaturated fatty acids (PUFA), docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), is beneficial to a variety of inflammatory disorders due, in part, to the ...

  12. Lipid Metabolism, Learning Ability and Potential Biomarkers for Atherosclerosis in Monk Parrots (Myiopsitta monachus) Fed N-3 Fatty Acids 

    E-Print Network [OSTI]

    Petzinger, Christina

    2012-07-16T23:59:59.000Z

    factors for atherosclerosis in avian species and learning ability, a series of studies were conducted in Monk parrots: 1) alterations comparing a high linoleic acid diet with -linolenic acid (ALA) diet on lipid metabolism, fatty acid conversions...

  13. Microbial engineering for the production of fatty acids and fatty acid derivatives

    DOE Patents [OSTI]

    Stephanopoulos, Gregory; Abidi, Syed Hussain Imam

    2014-07-01T23:59:59.000Z

    Some aspects of this invention relate to methods useful for the conversion of a carbon source to a biofuel or biofuel precursor using engineered microbes. Some aspects of this invention relate to the discovery of a key regulator of lipid metabolism in microbes. Some aspects of this invention relate to engineered microbes for biofuel or biofuel precursor production.

  14. Effects on milk yield and composition of infusions of volatile fatty acids and caseinate into

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    Effects on milk yield and composition of infusions of volatile fatty acids and caseinate isoenergetic infusions of either a low (17 mol/day of 64% acetic, 21% propionic and 15% butyric acids) or high that with infusions, energy and nitrogen needs were met and consisted of 60% maize silage, 10% hay, 21.5% energy

  15. Fatty acid ecology of plankton communities. Progress report, May 1, 1979-April 30, 1980

    SciTech Connect (OSTI)

    Jeffries, H P

    1980-01-01T23:59:59.000Z

    The principal hypothesis addressed in this study states that community variability is related to fatty acid structure. As a test of this idea, the zooplankton in three regimes of increasing physical severity (Block Island Sound, Narragansett Bay and Green Hill Pond) are being compared. Measurements were made on the physical environment, on standing crop and on fatty acid composition in both the phytoplankton-microzooplankton and macrozooplankton. Fatty acid variation in these communities displays a unique trajectory in time at each location. Environmental change and biochemical variability are directly related. The resulting biochemical message is complex but apparently highly informative. Patterns of variation in some fatty acids are affected most strongly by physical environmental parameters whereas the variation of other fatty acids is more responsive to differences in species composition, diversity and food web relationships. Taken together, these two aspects of biochemical pattern appear to characterize complex species assemblages. The result offers a new strategem for convenient assessment of the ever changing state in a natural community.

  16. Optimum Requirements for the Synthesis of Biodiesel Using Fatty Acid Distillates

    E-Print Network [OSTI]

    Akunna E. Ejele

    The optimum requirements of temperature, retention time, mole ratio of reactants and catalyst for the direct synthesis of biodiesel from fatty acid distillates of palm kernel oil using tetraoxosulphate (VI) acid as catalyst was studied. The following parameters were used for the efficient and economic production of biodiesel: eight (8) moles of methanol per mole of fatty acid, 0.06 mole of tetraoxosulphate (VI) acid per mole of fatty acid, a retention time of sixty (60) minutes and reaction temperature of 65 OC. And this gave a maximum percentage yield of 98.4. Other parameters obtained include: an acid value of 0.1683 mg KOH/g, iodine value of 15.3549, flash point of 209 OC, viscosity of 3.7957 mm2s-1, density of 0.8776 g cm-3, water content of 400.05 mg kg-1, soap content of 2.30 mg/kg, and ester content of 98.804 %. From the obtained parameters, the biodiesel produced from fatty acid distillates of palm kernel oil reaches prescribed international standards for biodiesel production.

  17. N-3 FATTY ACIDS AND JUVENILE TRAUMATIC BRAIN INJURY: EFFECTS OF DIETARY N-3 FATTY ACID CONTENT, N-3 FATTY ACID STATUS, AND ORALLY DOSED FISH OIL ON SENSORIMOTOR AND BIOCHEMICAL OUTCOMES

    E-Print Network [OSTI]

    Russell, Kristin L.

    2013-08-31T23:59:59.000Z

    MMP Matrix metalloproteinase MUFA Monounsaturated fatty acid NF-?B Nuclear factor kappa B NMDA N-methyl-D-aspartic acid NPD1 Neuroprotectin D1 xii Abbreviation Full Name PBF Phosphate buffered formalin PBS Phosphate buffered...). Transcription of a pro-MMP-2 and pro-MMP-9 is regulated by nuclear factor kappa B (NF-?B) (Gottschall and Deb, 1996). The pro- forms are cleaved to active forms by each other and other MMPs when necessary (Nagase et al., 2006). MMPs are endogenously...

  18. Determination of Free Fatty Acids and Triglycerides by Gas Chromatography Using Selective Esterification Reactions

    SciTech Connect (OSTI)

    Kail, Brian W.; Link, Dirk D.; Morreale, Bryan D

    2012-11-01T23:59:59.000Z

    A method for selectively determining both free fatty acids (FFA) and triacylglycerides (TAGs) in biological oils was investigated and optimized using gas chromatography after esterification of the target species to their corresponding fatty acid methyl esters (FAMEs). The method used acid catalyzed esterification in methanolic solutions under conditions of varying severity to achieve complete conversion of more reactive FFAs while preserving the concentration of TAGs. Complete conversion of both free acids and glycerides to corresponding FAMEs was found to require more rigorous reaction conditions involving heating to 120°C for up to 2 h. Method validation was provided using gas chromatography–flame ionization detection, gas chromatography–mass spectrometry, and liquid chromatography–mass spectrometry. The method improves on existing methods because it allows the total esterified lipid to be broken down by FAMEs contributed by FFA compared to FAMEs from both FFA and TAGs. Single and mixed-component solutions of pure fatty acids and triglycerides, as well as a sesame oil sample to simulate a complex biological oil, were used to optimize the methodologies. Key parameters that were investigated included: HCl-to-oil ratio, temperature and reaction time. Pure free fatty acids were found to esterify under reasonably mild conditions (10 min at 50°C with a 2.1:1 HCl to fatty acid ratio) with 97.6 ± 2.3% recovery as FAMEs, while triglycerides were largely unaffected under these reaction conditions. The optimized protocol demonstrated that it is possible to use esterification reactions to selectively determine the free acid content, total lipid content, and hence, glyceride content in biological oils. This protocol also allows gas chromatography analysis of FAMEs as a more ideal analyte than glyceride species in their native state.

  19. Effects of dietary fish oil on fatty acid composition and flavor of channel catfish

    E-Print Network [OSTI]

    Haynes, Kellie Cecile

    1989-01-01T23:59:59.000Z

    EFFECTS OF DIETARY FISH OIL ON FATTY ACID COMPOSITION AND FLAVOR OF CHANNEL CATFISH A Thesis by KELLIE CECILE HAYNES Submitted to the Office of Graduate Studies of Texas A8M University in partial fulfilment of the requirements for the degree... of MASTER OF SCIENCE December 1989 Major Subject: Food Science and Technology EFFECTS OF DIETARY FISH OIL ON FATTY ACID COMPOSITION AND FLAVOR OF CHANNEL CATFISH A thesis by KELLIE CECILE HAYNES Approved as to style and content by: J myT Ke...

  20. Novel short chain fatty acids restore chloride secretion in cystic fibrosis

    SciTech Connect (OSTI)

    Nguyen, Toan D. [Division of Gastroenterology, Department of Medicine, University of Washington and VA Puget Sound Health Care System, Seattle, WA 98108 (United States)]. E-mail: T1Nguyen@u.washington.edu; Kim, Ug-Sung [Division of Gastroenterology, Department of Medicine, University of Washington and VA Puget Sound Health Care System, Seattle, WA 98108 (United States); Perrine, Susan P. [Cancer Center, Departments of Pediatrics, Medicine, Pharmacology and Experimental Therapeutics, Boston University School of Medicine, Boston, MA 02118 (United States)

    2006-03-31T23:59:59.000Z

    Phenylalanine deletion at position 508 of the cystic fibrosis transmembrane conductance regulator ({delta}F508-CFTR), the most common mutation in cystic fibrosis (CF), causes a misfolded protein exhibiting partial chloride conductance and impaired trafficking to the plasma membrane. 4-Phenylbutyrate corrects defective {delta}F508-CFTR trafficking in vitro, but is not clinically efficacious. From a panel of short chain fatty acid derivatives, we showed that 2,2-dimethyl-butyrate (ST20) and {alpha}-methylhydrocinnamic acid (ST7), exhibiting high oral bioavailability and sustained plasma levels, correct the {delta}F508-CFTR defect. Pre-incubation ({>=}6 h) of CF IB3-1 airway cells with {>=}1 mM ST7 or ST20 restored the ability of 100 {mu}M forskolin to stimulate an {sup 125}I{sup -} efflux. This efflux was fully inhibited by NPPB, DPC, or glibenclamide, suggesting mediation through CFTR. Partial inhibition by DIDS suggests possible contribution from an additional Cl{sup -} channel regulated by CFTR. Thus, ST7 and ST20 offer treatment potential for CF caused by the {delta}F508 mutation.

  1. Cryptosporidium parvum: enhancing our understanding of its unique fatty acid metabolism and the elucidation of putative new inhibitors 

    E-Print Network [OSTI]

    Fritzler, Jason Michael

    2008-10-10T23:59:59.000Z

    of this parasitic protist is increasing at a rapid rate due to recent molecular and genetic advances. The topic of our research is in the area of C. parvum fatty acid metabolism, which is highly streamlined in this parasite. In addition to a type I fatty acid...

  2. Enrichment of canine gestation and lactation diets with n-3 polyunsaturated fatty acids to support neurologic development

    E-Print Network [OSTI]

    Heinemann, Kimberly Michele

    2005-11-01T23:59:59.000Z

    on the fatty acid composition of canine plasma phospholipids (PL) and milk during the gestation and lactation periods. The fatty acid composition of plasma PL and the retinal development of puppies reared on the same experimental diets as their mothers were...

  3. Novel Biosynthetic Pathway for Production of Fatty Acid Derived Molecules -

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level:Energy: Grid Integration Redefining What's Possible for Renewable Energy:Nanowire3627 Federal Register / Vol. 77, No. 23807 1- EnergyEnergy

  4. Induction of acid and oxidative reistance in Escherichia cole 0157:H7 by exposure to short-chain fatty acids

    E-Print Network [OSTI]

    Arnold, Carrie Nell

    2013-02-22T23:59:59.000Z

    Short-chain fatty acids (SCFAs) are commonly used as food preservatives to prevent microbial contamination of meat carcasses. However, the food-borne pathogen, E. coli O157:H7, is more resistant than other E. coli strains to SCFA treatment...

  5. Changes in the fatty acid composition of goat milk fat after a 48-hour fast

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    milking was analysed. A total of 10 samples was taken from each goat and cooled. Milk samples 1-6 wereChanges in the fatty acid composition of goat milk fat after a 48-hour fast Anne-Marie MASSART, B-9000 Ghent, Belgium. Summary. Five lactating goats were milked twice daily. After a control period

  6. EFFECT OF DIETARY FISH OIL ON THE FATTY ACID COMPOSITION AND PALATABILITY OF PIG TISSUES'

    E-Print Network [OSTI]

    organoleptic score' when the pigs were fed the oil containing diets to a market weight of 90.9 kg. (2) Removal the oil during an interval of time before the animals are marketed (Frazer, Stot- hart, and GutteridgeEFFECT OF DIETARY FISH OIL ON THE FATTY ACID COMPOSITION AND PALATABILITY OF PIG TISSUES' ROBERT R

  7. Stable carbon isotopic compositions of bacterial fatty acids in a seagrass dominated system

    E-Print Network [OSTI]

    Jones, Walter Brian

    2001-01-01T23:59:59.000Z

    as a proxy of organic carbon sources to the sediment. T. testudinum above ground tissues averaged -11.8±0.3[0/00] and benthic microalgae, as represented by the fatty acid 20:5[]3, averaged -20.5±0.6[0/00]. The TO¹³C from all habitats and depths were...

  8. Some characteristics of mitochondrial fatty acid oxidation in the liver of the neonatal pig : preliminary results

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    body uti- lization but to a low rate of hepatic ketone body production (Pégorier et al., 1983). The low rate of ketone body production also results from a limited capacity for fatty acid oxidation associated to Mersmann et al. (1972). The mitochondrial pellet was suspended in sucrose-Tris buffer to give

  9. Sequential fatty acid analysis of a peat core covering the last two millennia (Tritrivakely lake, Madagascar)

    E-Print Network [OSTI]

    Boyer, Edmond

    Sequential fatty acid analysis of a peat core covering the last two millennia (Tritrivakely lake, Université d'Orléans, BP 6759, 45067 Orléans Cedex 2, France Abstract Seven samples from a 1 m long peat core targets because of their dominant or even exclusive OM content, peat deposits have received relatively

  10. The effect of valinomycin in fibroblasts from patients with fatty acid oxidation disorders

    SciTech Connect (OSTI)

    Ndukwe Erlingsson, Uzochi Chimdinma [Division of Medical Genetics, Department of Pediatrics, University of Utah, 2C412 SOM, 50 North Mario Capecchi Drive, Salt Lake City, UT 84132 (United States)] [Division of Medical Genetics, Department of Pediatrics, University of Utah, 2C412 SOM, 50 North Mario Capecchi Drive, Salt Lake City, UT 84132 (United States); Iacobazzi, Francesco [Division of Medical Genetics, Department of Pediatrics, University of Utah, 2C412 SOM, 50 North Mario Capecchi Drive, Salt Lake City, UT 84132 (United States) [Division of Medical Genetics, Department of Pediatrics, University of Utah, 2C412 SOM, 50 North Mario Capecchi Drive, Salt Lake City, UT 84132 (United States); Department of Basic Medical Sciences, University of Bari, Piazza Giulio Cesare 11, Policlinico, I-70124 Bari (Italy); Liu, Aiping [ARUP Institute for Clinical and Experimental Pathology, ARUP Laboratories, 500 Chipeta Way, Salt Lake City, UT 84108 (United States)] [ARUP Institute for Clinical and Experimental Pathology, ARUP Laboratories, 500 Chipeta Way, Salt Lake City, UT 84108 (United States); Ardon, Orly; Pasquali, Marzia [Division of Medical Genetics, Department of Pediatrics, University of Utah, 2C412 SOM, 50 North Mario Capecchi Drive, Salt Lake City, UT 84132 (United States) [Division of Medical Genetics, Department of Pediatrics, University of Utah, 2C412 SOM, 50 North Mario Capecchi Drive, Salt Lake City, UT 84132 (United States); ARUP Institute for Clinical and Experimental Pathology, ARUP Laboratories, 500 Chipeta Way, Salt Lake City, UT 84108 (United States); Department of Pathology, University of Utah, Salt Lake City, UT 84132 (United States); Longo, Nicola, E-mail: Nicola.Longo@hsc.utah.edu [Division of Medical Genetics, Department of Pediatrics, University of Utah, 2C412 SOM, 50 North Mario Capecchi Drive, Salt Lake City, UT 84132 (United States) [Division of Medical Genetics, Department of Pediatrics, University of Utah, 2C412 SOM, 50 North Mario Capecchi Drive, Salt Lake City, UT 84132 (United States); ARUP Institute for Clinical and Experimental Pathology, ARUP Laboratories, 500 Chipeta Way, Salt Lake City, UT 84108 (United States); Department of Pathology, University of Utah, Salt Lake City, UT 84132 (United States)

    2013-08-09T23:59:59.000Z

    Highlights: •Valinomycin can cause mitochondrial stress and stimulate fatty acid oxidation. •Cells with VLCAD deficiency fail to increase fatty acid oxidation in response to valinomycin. •Response to valinomycin can help in the diagnosis of VLCAD deficiency. -- Abstract: Disorders of the carnitine cycle and of the beta oxidation spiral impair the ability to obtain energy from fats at time of fasting and stress. This can result in hypoketotic hypoglycemia, cardiomyopathy, cardiac arrhythmia and other chronic medical problems. The in vitro study of fibroblasts from patients with these conditions is impaired by their limited oxidative capacity. Here we evaluate the capacity of valinomycin, a potassium ionophore that increases mitochondrial respiration, to increase the oxidation of fatty acids in cells from patients with inherited fatty acid oxidation defects. The addition of valinomycin to fibroblasts decreased the accumulation of the lipophilic cation tetraphenylphosphonium (TPP{sup +}) at low concentrations due to the dissipation of the mitochondrial membrane potential. At higher doses, valinomycin increased TPP{sup +} accumulation due to the increased potassium permeability of the plasma membrane and subsequent cellular hyperpolarization. The incubation of normal fibroblasts with valinomycin increased [{sup 14}C]-palmitate oxidation (measured as [{sup 14}C]O{sub 2} release) in a dose-dependent manner. By contrast, valinomycin failed to increase palmitate oxidation in fibroblasts from patients with very long chain acyl CoA dehydrogenase (VLCAD) deficiency. This was not observed in fibroblasts from patients heterozygous for this condition. These results indicate that valinomycin can increase fatty acid oxidation in normal fibroblasts and could be useful to differentiate heterozygotes from patients affected with VLCAD deficiency.

  11. Guiding optimal biofuels : a comparative analysis of the biochemical production of ethanol and fatty acid ethyl esters from switchgrass.

    SciTech Connect (OSTI)

    Paap, Scott M.; West, Todd H.; Manley, Dawn Kataoka; Dibble, Dean C.; Simmons, Blake Alexander; Steen, Eric J. [Joint BioEnergy Institute, Emeryville, CA; Beller, Harry R. [Lawrence Berkeley National Laboratory, Berkeley, CA; Keasling, Jay D. [Lawrence Berkeley National Laboratory, Berkeley, CA; Chang, Shiyan [Tsinghua University, Beijing, PR China

    2013-01-01T23:59:59.000Z

    In the current study, processes to produce either ethanol or a representative fatty acid ethyl ester (FAEE) via the fermentation of sugars liberated from lignocellulosic materials pretreated in acid or alkaline environments are analyzed in terms of economic and environmental metrics. Simplified process models are introduced and employed to estimate process performance, and Monte Carlo analyses were carried out to identify key sources of uncertainty and variability. We find that the near-term performance of processes to produce FAEE is significantly worse than that of ethanol production processes for all metrics considered, primarily due to poor fermentation yields and higher electricity demands for aerobic fermentation. In the longer term, the reduced cost and energy requirements of FAEE separation processes will be at least partially offset by inherent limitations in the relevant metabolic pathways that constrain the maximum yield potential of FAEE from biomass-derived sugars.

  12. Electrochemical method for producing a biodiesel mixture comprising fatty acid alkyl esters and glycerol

    DOE Patents [OSTI]

    Lin, YuPo J; St. Martin, Edward J

    2013-08-13T23:59:59.000Z

    The present invention relates to an integrated method and system for the simultaneous production of biodiesel from free fatty acids (via esterification) and from triglycerides (via transesterification) within the same reaction chamber. More specifically, one preferred embodiment of the invention relates to a method and system for the production of biodiesel using an electrodeionization stack, wherein an ion exchange resin matrix acts as a heterogeneous catalyst for simultaneous esterification and transesterification reactions between a feedstock and a lower alcohol to produce biodiesel, wherein the feedstock contains significant levels of free fatty acid. In addition, because of the use of a heterogeneous catalyst, the glycerol and biodiesel have much lower salt concentrations than raw biodiesel produced by conventional transesterification processes. The present invention makes it much easier to purify glycerol and biodiesel.

  13. Effect of dietary polyunsaturated fatty acids and related nutrients on sebum lipids, and skin and hair coat condition in canines 

    E-Print Network [OSTI]

    Kirby, Naomi Anne

    2005-02-17T23:59:59.000Z

    A study was performed to investigate the effect of diets rich in polyunsaturated fatty acids and other related nutrients, in the effort to improve skin and hair coat conditions in canines. The study included 24 dogs fed a ...

  14. Br J Nutr . Author manuscript Maternal fatty acid intake and fetal growth: evidence for an association in

    E-Print Network [OSTI]

    -Guillain 1 , Olivier Thi baugeorgesé 2 , Val rie Gouaé 3 , Guillaume Magnin 3 , Michel Schweitzer 2 , Monique and lactation. Essential fatty acids (EFA) play a major role during pregnancy. They provide the precursors

  15. The effect of dietary n-3 polyunsaturated fatty acids on T cell subset activation-induced cell death 

    E-Print Network [OSTI]

    Switzer, Kirsten Collette

    2004-11-15T23:59:59.000Z

    Dietary n-3 polyunsaturated fatty acids (PUFA) have been shown to potently attenuate T cell-mediated inflammation, in part, by suppressing T cell activation and proliferation. Apoptosis is an important mechanism for ...

  16. Effect of consuming ground beef of differing monounsaturated fatty acid content on atherosclerotic cardiovascular disease risk factors in healthy men

    E-Print Network [OSTI]

    Cao, Xiaojuan

    2009-05-15T23:59:59.000Z

    Atherosclerotic cardiovascular disease (ASCVD) is currently the most common cause of death in the United States. Some dietary factors contribute importantly to ASCVD and other factors can reduce risk of ASCVD. Oleic acid is a monounsaturated fatty...

  17. Indices of stress in exercising horses fed diets containing varying amounts of omega-6 and omega-3 polyunsaturated fatty acids 

    E-Print Network [OSTI]

    Howard, Alicia Dawn

    2005-11-01T23:59:59.000Z

    Dietary omega-3 polyunsaturated fatty acids have shown substantial benefits in humans including lowered serum cholesterol, blood pressure and indices of stress. The caloric and extracaloric benefits of feeding fat supplemented diets to performance...

  18. A study on the adsorbability characteristics of unsaturated fatty acids

    E-Print Network [OSTI]

    Williams, William Thomas

    1951-01-01T23:59:59.000Z

    tatty acids (AOy39) ~ The triglycerides vere least adsorbed, ut in re: 'ral it wns found that alumina adsorbs the c-&asti tuerts of high molecular weight while the reverse ts tz. !e of silica eel, and that &. atter separation is obta'ncd?ith aluz... of the solven? nnd the solute, "-'he 'fiselius method is also completely independent of the color oi' the adsorbent, thus allow&n;? thc use of &ctive &. arbon which seems to !?e espeoio. !ly suite! lc for &sany senarations, ':han-es in c 'ncontration of thc...

  19. ANALYSIS OF RICIN TOXIN PREPARATIONS FOR CARBOHYDRATE AND FATTY ACID ABUNDANCE AND ISOTOPE RATIO INFORMATION

    SciTech Connect (OSTI)

    Wunschel, David S.; Kreuzer-Martin, Helen W.; Antolick, Kathryn C.; Colburn, Heather A.; Moran, James J.; Melville, Angela M.

    2009-12-01T23:59:59.000Z

    This report describes method development and preliminary evaluation for analyzing castor samples for signatures of purifying ricin. Ricin purification from the source castor seeds is essentially a problem of protein purification using common biochemical methods. Indications of protein purification will likely manifest themselves as removal of the non-protein fractions of the seed. Two major, non-protein, types of biochemical constituents in the seed are the castor oil and various carbohydrates. The oil comprises roughly half the seed weight while the carbohydrate component comprises roughly half of the remaining “mash” left after oil and hull removal. Different castor oil and carbohydrate components can serve as indicators of specific toxin processing steps. Ricinoleic acid is a relatively unique fatty acid in nature and is the most abundant component of castor oil. The loss of ricinoleic acid indicates a step to remove oil from the seeds. The relative amounts of carbohydrates and carbohydrate-like compounds, including arabinose, xylose, myo-inositol fucose, rhamnose, glucosamine and mannose detected in the sample can also indicate specific processing steps. For instance, the differential loss of arabinose relative to mannose and N-acetyl glucosamine indicates enrichment for the protein fraction of the seed using protein precipitation. The methods developed in this project center on fatty acid and carbohydrate extraction from castor samples followed by derivatization to permit analysis by gas chromatography-mass spectrometry (GC-MS). Method descriptions herein include: the source and preparation of castor materials used for method evaluation, the equipment and description of procedure required for chemical derivatization, and the instrument parameters used in the analysis. Two types of derivatization methods describe analysis of carbohydrates and one procedure for analysis of fatty acids. Two types of GC-MS analysis is included in the method development, one employing a quadrupole MS system for compound identification and an isotope ratio MS for measuring the stable isotope ratios of deuterium and hydrogen (D/H) in fatty acids. Finally, the method for analyzing the compound abundance data is included. This study indicates that removal of ricinoleic acid is a conserved consequence of each processing step we tested. Furthermore, the stable isotope D/H ratio of ricinoleic acid distinguished between two of the three castor seed sources. Concentrations of arabinose, xylose, mannose, glucosamine and myo-inositol differentiated between crude or acetone extracted samples and samples produced by protein precipitation. Taken together these data illustrate the ability to distinguish between processes used to purify a ricin sample as well as potentially the source seeds.

  20. The effect of dietary n-3 polyunsaturated fatty acids on T cell subset activation-induced cell death

    E-Print Network [OSTI]

    Switzer, Kirsten Collette

    2004-11-15T23:59:59.000Z

    oil (FO), which is enriched with the two n-3 polyunsaturated fatty acids (PUFA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). The majority of clinical studies have focused on the use of FO supplementation in rheumatoid arthritis... the methyl end. Of the n-3 PUFA class, EPA (20:5n-3) and DHA are found in cold-water fatty fish while alphalinolenic acid (ALA, 18:3n-3) is found in green leafy vegetables, and walnut and flaxseed oils. n-6 PUFA are primarily found in animal fats...

  1. Crystal structure of FAS thioesterase domain with polyunsaturated fatty acyl adduct and inhibition by dihomo-[gamma]-linolenic acid

    SciTech Connect (OSTI)

    Zhang, Wei; Chakravarty, Bornali; Zheng, Fei; Gu, Ziwei; Wu, Hongmei; Mao, Jianqiang; Wakil, Salih J.; Quiocho, Florante A. (Baylor)

    2012-05-29T23:59:59.000Z

    Human fatty acid synthase (hFAS) is a homodimeric multidomain enzyme that catalyzes a series of reactions leading to the de novo biosynthesis of long-chain fatty acids, mainly palmitate. The carboxy-terminal thioesterase (TE) domain determines the length of the fatty acyl chain and its ultimate release by hydrolysis. Because of the upregulation of hFAS in a variety of cancers, it is a target for antiproliferative agent development. Dietary long-chain polyunsaturated fatty acids (PUFAs) have been known to confer beneficial effects on many diseases and health conditions, including cancers, inflammations, diabetes, and heart diseases, but the precise molecular mechanisms involved have not been elucidated. We report the crystal structure of the hFAS TE domain covalently modified and inactivated by methyl {gamma}-linolenylfluorophosphonate. Whereas the structure confirmed the phosphorylation by the phosphonate head group of the active site serine, it also unexpectedly revealed the binding of the 18-carbon polyunsaturated {gamma}-linolenyl tail in a long groove-tunnel site, which itself is formed mainly by the emergence of an {alpha} helix (the 'helix flap'). We then found inhibition of the TE domain activity by the PUFA dihomo-{gamma}-linolenic acid; {gamma}- and {alpha}-linolenic acids, two popular dietary PUFAs, were less effective. Dihomo-{gamma}-linolenic acid also inhibited fatty acid biosynthesis in 3T3-L1 preadipocytes and selective human breast cancer cell lines, including SKBR3 and MDAMB231. In addition to revealing a novel mechanism for the molecular recognition of a polyunsaturated fatty acyl chain, our results offer a new framework for developing potent FAS inhibitors as therapeutics against cancers and other diseases.

  2. Milk fat composition of a lactating dairy cow as influenced by abomasal infusion of various long chain fatty acids 

    E-Print Network [OSTI]

    Switzer, Leonard Anderson

    1971-01-01T23:59:59.000Z

    MILK FAT COMPOS ITIQN QF A LACTATING DAIRY COLI AS INFLUENCED BY ABOMASAL INFUSION OF VARIOUS LONG CHAIN FATTY ACIDS A Thesis by LEONARD ANDERSON S4/ITZER Submitted to the Graduate College of Texas ARM University in partial fulfillment... of the requirement for the degree of MASTER OF SCIENCE August 1971 Major Subject: Dairy Science MILK FAT COMPOSITION OF A LACTATING DAIRY COIJ AS INFLUENCED BY ABOMASAL INF'US ION OF VARIOUS LONG CHAIN FATTY ACIDS A Thesis by LEONARD ANDERSON SIJITZER...

  3. The role of apaptosis in the suppression of lymphoproliferation which is observed in animals fed diets rich in n-3 polyunsaturated fatty acids

    E-Print Network [OSTI]

    Shawver, Paula Ann

    2013-02-22T23:59:59.000Z

    for many types of inflammation. Previously, we demonstratied that splenocyte cultures of mice fed diets reich in the n-3 fatty acid eicosapentanoic acid (EPA) undergo a higher degree of apoptosis, or programmed cell death, upon stimulation, than celks from...

  4. Competition between Atmospherically Relevant Fatty Acid Monolayers at the Air/Water Laura F. Voss, Christopher M. Hadad,* and Heather C. Allen*

    E-Print Network [OSTI]

    Competition between Atmospherically Relevant Fatty Acid Monolayers at the Air/Water Interface Laura F. Voss, Christopher M. Hadad,* and Heather C. Allen* Department of Chemistry, The Ohio State Uni Competition and oxidation of fatty acids spread at the air/water interface were investigated using surface

  5. The selective effect of dietary n-3 polyunsaturated fatty acids on murine Th1 and Th2 cell development 

    E-Print Network [OSTI]

    Zhang, Ping

    2006-10-30T23:59:59.000Z

    To examine how dietary n-3 polyunsaturated fatty acids affect Th2 cell development, female C57BL/6 mice were fed a washout corn oil (CO) diet for 1 wk followed by 2 wk of either the same CO diet or a fish oil (FO) diet. ...

  6. Multiscale structures of lipids in foods & Fatty acid metabolism Revised version submitted to Progress in Lipid Research March 2013

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    -Ferrand, France; * RMT LISTRAL: Mixed Technological Network combining academic and industrial partners, devoted On a nutritional standpoint, lipids are now being studied beyond their energy content and fatty acid (FA) profiles in native and formulated food products and (iv) the food matrix. Further work should be accomplished now

  7. Enrichment of plasma lipid subfractions with varying dietary levels of N-3 fatty acids in the canine model

    E-Print Network [OSTI]

    Spencer, Angela Leah

    1999-01-01T23:59:59.000Z

    In an attempt to better understand how varying levels of dietary n-3 fatty acids affected enrichment of plasma lipid subfractions, the current study was undertaken. Adult dogs were fed one of two basal diets with the addition of beef tallow...

  8. Fatty acid oxidation and ketogenesis during development J. GIRARD, P. H. DUE, P. FERR J. P. PGORIER F. ESCRIVA

    E-Print Network [OSTI]

    Boyer, Edmond

    (a high-fat diet) develops rapidly in various tissues and remains very high throughout the suckling a high-fat to a high- carbohydrate diet is attended by a progressive decrease in the ketogenic capacity-fat diet. The principal organs responsible for fatty acid oxidation are the heart, skeletal muscles, liver

  9. Chemistry of natural fuel: Use of wastes of synthetic fatty acid production for obtaining water-bitumen emulsions

    SciTech Connect (OSTI)

    Syroezhko, A.M.; Antipova, E.I.; Paukku, A.N. [St. Petersburg Technological Inst. (Russian Federation)

    1995-12-10T23:59:59.000Z

    The possibility of producing water-emulsion waterproofing mastic and waterproofing coating based on bitumen, rubber crumb, and bottoms from production of synthetic fatty acids was studied. The physicochemical properties (softening point, ductility, sorptive properties, and friability) of the waterproofing coating based on a water-emulsion mastic were measured.

  10. The selective effect of dietary n-3 polyunsaturated fatty acids on murine Th1 and Th2 cell development

    E-Print Network [OSTI]

    Zhang, Ping

    2006-10-30T23:59:59.000Z

    To examine how dietary n-3 polyunsaturated fatty acids affect Th2 cell development, female C57BL/6 mice were fed a washout corn oil (CO) diet for 1 wk followed by 2 wk of either the same CO diet or a fish oil (FO) diet. CD4+ T cells were isolated...

  11. Effects of digestive infusions of volatile fatty acids or glucose on food intake in lactating or dry cows

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    Effects of digestive infusions of volatile fatty acids or glucose on food intake in lactating lactating (30 kg of milk/d) and 3 non pregnant dry dairy cows. The effect of each energetic infusion) control solutions of VFA and glucose treatments were infused at the same vol, pH and osmolarity

  12. Effects of Fatty Acids on Gene Expression and Lipid Metabolism in Bovine Intramuscular and Subcutaneous Adipose Tissues

    E-Print Network [OSTI]

    Silvey, David Tyrone

    2012-10-19T23:59:59.000Z

    ) and intramuscular (IM) adipose tissues. Angus steers were harvested at 12, 14, and 16 mo of age, and IM and SC adipose tissue explants from the 8-11th thoracic rib region were dissected and cultured in media. Media contained no supplemental fatty acids or 40 micro...

  13. The influence of molecular structure of fatty acid monoalkyl esters on diesel combustion

    SciTech Connect (OSTI)

    Schoenborn, Alessandro; Ladommatos, Nicos [Department of Mechanical Engineering, University College London, Torrington Place, London WC1E 7JE (United Kingdom); Williams, John; Allan, Robert; Rogerson, John [BP Oil UK Limited, Technology Centre, Whitchurch Hill, Pangbourne, Reading, RG8 7QR (United Kingdom)

    2009-07-15T23:59:59.000Z

    The subject of this paper is a series of experiments conducted on a single-cylinder research engine investigating the influence of molecular structure on the combustion behaviour of fatty acid alcohol ester (biodiesel) molecules under diesel engine conditions. The fuels employed in these experiments comprised various samples of pure individual fatty acid alcohol ester molecules of different structure, as well as several mixtures of such molecules. The latter consisted in biodiesel fuels produced by the transesterification of naturally occurring plant oils or animal fat with a monohydric alcohol. It was observed that the molecular structure of the fuel significantly influenced the formation of NO{sub x} and particulate matter and their respective concentration in the exhaust gas. The influence on the formation of NO{sub x} in particular, appeared to be exerted first through the effect which the molecular structure had on the auto-ignition delay occurring after the fuel was injected into the combustion chamber, and second through the flame temperature at which the various molecules burned. The emission of particulates on the other hand showed correlation with the number of double bonds in the fuel molecules for the case of larger accumulation mode particles, and with the boiling point of the fuel samples for the case of the smaller, nucleation mode particles. The effect of ignition delay on the exhaust emissions of these pollutants was isolated by adding the ignition promoting molecule 2-ethylhexyl nitrate to some of the fuel samples in closely specified concentrations, so as to equalise the ignition delay for the relevant fuel samples. The removal of the ignition delay as a main influence on the combustion process enabled the observation of the lesser effects of adiabatic flame temperature. (author)

  14. Membrane extraction with thermodynamically unstable diphosphonic acid derivatives

    DOE Patents [OSTI]

    Horwitz, E.P.; Gatrone, R.C.; Nash, K.L.

    1997-10-14T23:59:59.000Z

    Thermodynamically-unstable complexing agents which are diphosphonic acids and diphosphonic acid derivatives (or sulphur containing analogs), like carboxyhydroxymethanediphosphonic acid and vinylidene-1,1-diphosphonic acid, are capable of complexing with metal ions, and especially metal ions in the II, III, IV, V and VI oxidation states, to form stable, water-soluble metal ion complexes in moderately alkaline to highly-acidic media. However, the complexing agents can be decomposed, under mild conditions, into non-organic compounds which, for many purposes are environmentally-nondamaging compounds thereby degrading the complex and releasing the metal ion for disposal or recovery. Uses for such complexing agents as well as methods for their manufacture are also described. 1 fig.

  15. Membrane extraction with thermodynamically unstable diphosphonic acid derivatives

    DOE Patents [OSTI]

    Horwitz, Earl Philip (Argonne, IL); Gatrone, Ralph Carl (Argonne, IL); Nash, Kenneth LaVerne (Argonne, IL)

    1997-01-01T23:59:59.000Z

    Thermodynamically-unstable complexing agents which are diphosphonic acids and diphosphonic acid derivatives (or sulphur containing analogs), like carboxyhydroxymethanediphosphonic acid and vinylidene-1,1-diphosphonic acid, are capable of complexing with metal ions, and especially metal ions in the II, III, IV, V and VI oxidation states, to form stable, water-soluble metal ion complexes in moderately alkaline to highly-acidic media. However, the complexing agents can be decomposed, under mild conditions, into non-organic compounds which, for many purposes are environmentally-nondamaging compounds thereby degrading the complex and releasing the metal ion for disposal or recovery. Uses for such complexing agents as well as methods for their manufacture are also described.

  16. Extracting metal ions with diphosphonic acid, or derivative thereof

    DOE Patents [OSTI]

    Horwitz, E.P.; Gatrone, R.C.; Nash, K.L.

    1994-07-26T23:59:59.000Z

    Thermodynamically-unstable complexing agents which are diphosphonic acids and diphosphonic acid derivatives (or sulfur containing analogs), like carboxyhydroxymethanediphosphonic acid and vinylidene-1,1-diphosphonic acid, are capable of complexing with metal ions, and especially metal ions in the II, III, IV, V and VI oxidation states, to form stable, water-soluble metal ion complexes in moderately alkaline to highly-acidic media. However, the complexing agents can be decomposed, under mild conditions, into non-organic compounds which, for many purposes are environmentally-nondamaging compounds thereby degrading the complex and releasing the metal ion for disposal or recovery. Uses for such complexing agents as well as methods for their manufacture are also described. 1 fig.

  17. Activation of type 2 cannabinoid receptors (CB2R) promotes fatty acid oxidation through the SIRT1/PGC-1? pathway

    SciTech Connect (OSTI)

    Zheng, Xuqin [Department of Endocrinology, First Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu Province 210029 (China)] [Department of Endocrinology, First Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu Province 210029 (China); Sun, Tao [Department of Neurology, Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu Province 210002 (China)] [Department of Neurology, Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu Province 210002 (China); Wang, Xiaodong, E-mail: xdwang666@hotmail.com [Department of Endocrinology, First Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu Province 210029 (China)] [Department of Endocrinology, First Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu Province 210029 (China)

    2013-07-05T23:59:59.000Z

    Highlights: •TC, a CB2R specific agonist, stimulates SIRT1 activity by PKA/CREB pathway. •TC promotes PGC-1? transcriptional activity by increasing its deacetylation. •TC increases the expression of genes linked to FAO and promotes the rate of FAO. •The effects of TC in FAO are dependent on CB2R. •Suggesting CB2R as a target to treat diseases with lipid dysregulation. -- Abstract: Abnormal fatty acid oxidation has been associated with obesity and type 2 diabetes. At the transcriptional level, peroxisome proliferator-activated receptor-gamma coactivator 1? (PGC-1?) has been reported to strongly increase the ability of hormone nuclear receptors PPAR? and ERR? to drive transcription of fatty acid oxidation enzymes. In this study, we report that a specific agonist of the type 2 cannabinoid receptor (CB2R) can lead to fatty acid oxidation through the PGC-1? pathway. We have found that CB2R is expressed in differentiated C2C12 myotubes, and that use of the specific agonist trans-caryophyllene (TC) stimulates sirtuin 1 (SIRT1) deacetylase activity by increasing the phosphorylation of cAMP response element-binding protein (CREB), thus leading to increased levels of PGC-1? deacetylation. This use of TC treatment increases the expression of genes linked to the fatty acid oxidation pathway in a SIRT1/PGC-1?-dependent mechanism and also drastically accelerates the rate of complete fatty acid oxidation in C2C12 myotubes, neither of which occur when CB2R mRNA is knocked down using siRNA. These results reveal that activation of CB2R by a selective agonist promotes lipid oxidation through a signaling/transcriptional pathway. Our findings imply that pharmacological manipulation of CB2R may provide therapeutic possibilities to treat metabolic diseases associated with lipid dysregulation.

  18. Extracorporeal membrane oxygenation promotes long chain fatty...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    membrane oxygenation promotes long chain fatty acid oxidation in the immature swine heart in vivo. Extracorporeal membrane oxygenation promotes long chain fatty acid oxidation...

  19. Intestinal-fatty acid binding protein and lipid transport in human intestinal epithelial cells

    SciTech Connect (OSTI)

    Montoudis, Alain [Department of Nutrition, Universite de Montreal and Research Center, CHU Sainte Justine, 3175 Cote Ste-Catherine, Montreal, Que., H3T 1C5 (Canada); Delvin, Edgard [Department of Biochemistry, Universite de Montreal and Research Center, CHU Sainte Justine, 3175 Cote Ste-Catherine, Montreal, Que., H3T 1C5 (Canada); Canadian Institute of Health Research, Group of the Functional Development and Physiopathology of the Digestive Tract, and Department of Anatomy and Cellular Biology, Faculty of Medicine and Health Sciences, Universite de Sherbrooke, Sherbrooke, Que., Canada J1H 5N4 (Canada); Menard, Daniel [Department of Pathology and Cell Biology, Universite de Montreal and Research Center, CHU Sainte Justine, 3175 Cote Ste-Catherine, Montreal, Que., H3T 1C5 (Canada); Canadian Institute of Health Research, Group of the Functional Development and Physiopathology of the Digestive Tract, and Department of Anatomy and Cellular Biology, Faculty of Medicine and Health Sciences, Universite de Sherbrooke, Sherbrooke, Que., J1H 5N4 (Canada)] (and others)

    2006-01-06T23:59:59.000Z

    Intestinal-fatty acid binding protein (I-FABP) is a 14-15 kDa cytoplasmic molecule highly expressed in the enterocyte. Although different functions have been proposed for various FABP family members, the specific function of I-FABP in human intestine remains unclear. Here, we studied the role of I-FABP in molecularly modified normal human intestinal epithelial cells (HIEC-6). cDNA transfection resulted in 90-fold I-FABP overexpression compared to cells treated with empty pQCXIP vector. The high-resolution immunogold technique revealed labeling mainly in the cytosol and confirmed the marked phenotype abundance of I-FABP in cDNA transfected cells. I-FABP overexpression was not associated with alterations in cell proliferation and viability. Studies using these transfected cells cultured with [{sup 14}C]oleic acid did not reveal higher efficiency in de novo synthesis or secretion of triglycerides, phospholipids, and cholesteryl esters compared to cells treated with empty pQCXIP vector only. Similarly, the incubation with [{sup 35}S]methionine did not disclose a superiority in the biogenesis of apolipoproteins (apo) A-I, A-IV, B-48, and B-100. Finally, cells transfected with I-FABP did not exhibit an increased production of chylomicrons, VLDL, LDL, and HDL. Our observations establish that I-FABP overexpression in normal HIEC-6 is not related to cell proliferation, lipid esterification, apo synthesis, and lipoprotein assembly, and, therefore, exclude its role in intestinal fat transport.

  20. The effect of omega-3 and omega-6 polyunsaturated fatty acids on illness in children up to 4 years of age

    E-Print Network [OSTI]

    Foiles, Amanda

    2011-05-01T23:59:59.000Z

    OBJECTIVES: Supplementation of omega-3 (n-3) and omega-6 (n-6) long-chain polyunsaturated fatty acids (LCPUFA) during the first year of life has been associated with a decreased incidence of illness in children. The need ...

  1. The effect of different levels of forage and fish meal on the live performance and rumen volatile fatty acid concentation of heifers fed high molasses diets 

    E-Print Network [OSTI]

    Estrada, Sergio

    1973-01-01T23:59:59.000Z

    of differ- ent-carbohydrates on fatty acid production. His work indicated that sugars, beyond a certain concentration, depress cellulose digestion mo- re than equivalent concentration of starch. Depression of cellulose di- gestion was accompanied by a...

  2. The effect of polyunsaturated fatty acid peroxidation on cholesterol concentrations in plasma, aorta, liver, and spleen of the guinea pig

    E-Print Network [OSTI]

    Corrigan, Sheila Marie

    1986-01-01T23:59:59.000Z

    Biochemistry and Metabolism with Clinical Applications (Linder, M. , ed. ), pp. 33-50, Elsevier Science Publishing Company, Inc. , New York. 20. Stocks, J. & Dormandy, T. (1971) The autoxidation of human red cells lipids induced by 36 hydrogen peroxide...THE EFFECT OF POLYUNSATURATED FATTY ACID PEROXIDATION ON CHOLESTEROL CONCENTRAT IONS IN PLASMA AORTA g LIVER g AND SPLEEN OF THE GUINEA PIG A Thesis by SHEILA MARIE CORRIGAN Submitted to the Graduate College of Texas A&M University...

  3. Enrichment of canine gestation and lactation diets with n-3 polyunsaturated fatty acids to support neurologic development 

    E-Print Network [OSTI]

    Heinemann, Kimberly Michele

    2005-11-01T23:59:59.000Z

    ENRICHMENT OF CANINE GESTATION AND LACTATION DIETS WITH N-3 POLYUNSATURATED FATTY ACIDS TO SUPPORT NEUROLOGIC DEVELOPMENT A Dissertation by KIMBERLY MICHELE HEINEMANN Submitted to the Office of Graduate Studies of Texas A... NEUROLOGIC DEVELOPMENT A Dissertation by KIMBERLY MICHELE HEINEMANN Submitted to the Office of Graduate Studies of Texas A&M University in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY Approved as to style...

  4. Dietary polyunsaturated fatty acids modify plasma lipids and red blood cell membrane composition but do not induce ?6 desaturase mediated conversions in the domestic feline 

    E-Print Network [OSTI]

    McClure, Melena Kathleen

    2009-05-15T23:59:59.000Z

    This study explored the effects of dietary unsaturated fatty acids on feline lipid metabolism. It was hypothesized that high dietary linoleic acid (18:2n-6, LA) would enhance conversion to arachidonic acid (20:4n-6), enrichment of dietary long chain...

  5. Milk fat composition of a lactating dairy cow as influenced by abomasal infusion of various long chain fatty acids

    E-Print Network [OSTI]

    Switzer, Leonard Anderson

    1971-01-01T23:59:59.000Z

    acids of milk comes from blood acetate, which contributes ma. inly to the fatty acids containing from to 12 carbon atoms. Shaw and co-workers (44) demonstrated that 5- hydroxybutyrate was absorbed in significant amounts by the normal lactating udder... and abbreviations used will be as normal milking (NM), residual milking (SM), and hourly samples as 1 (1PI), 2 (2PI), 3 (3M), (4M), 5 (5PI)~ 6 (6M), 7 (7M)~ 8 (BPI), 9 (9M) and 10 (10M) hr after infusion. Blood samples were collected immediately before...

  6. Synthesis of pyroglutamic acid derivatives via double Michael addition reactions of alkynones. 

    E-Print Network [OSTI]

    Scansetti, Myriam

    2009-01-01T23:59:59.000Z

    I. Synthesis of pyroglutamic acid derivatives via double Michael reactions of alkynones Pyroglutamic acids and their derivatives are common structural units of widespread chemical significance and they have been heavily ...

  7. Effect of somatostatin on nonesterified fatty acid levels modifies glucose homeostasis during fasting

    SciTech Connect (OSTI)

    Hendrick, G.K.; Frizzell, R.T.; Cherrington, A.D. (Vanderbilt Univ. School of Medicine, Nashville, TN (USA))

    1987-10-01T23:59:59.000Z

    In the 7-days fasted conscious dog, unlike the postabsorptive conscious dog, somatostatin infusion results in decreased levels of nonesterified fatty acids (NEFA) and increased glucose utilization (R{sub d}) even when insulin and glucagon levels are held constant. The aim of this study was to determine whether NEFA replacement in such animals would prevent the increase in R{sub d}. In each of three protocols there was an 80-min tracer equilibration period, a 40-min basal period, and a 3-h test period. During the test period in the first protocol saline was infused, in the second protocol somatostatin was infused along with intraportal replacement amounts of insulin and glucagon (hormone replacement), while in the third protocol somatostatin plus the pancreatic hormones were infused with concurrent heparin plus Intralipid infusion. Glucose turnover was assessed using (3-{sup 3}H)glucose. The peripheral levels of insulin, glucagon, and glucose were similar and constant in all three protocols; however, during somatostatin infusion, exogenous glucose infusion was necessary to maintain euglycemia. The NEFA level was constant during saline infusion and decreased in the hormone replacement protocol. In the hormone replacement plus NEFA protocol, the NEFA level did not change during the first 90-min period and then increased during the second 90-min period. After a prolonged fast in the dog, (1) somatostatin directly or indirectly inhibits adipose tissue NEFA release and causes a decrease in the plasma NEFA level, and (2) this decrease in the NEFA level causes an increase in R{sub d}.

  8. THYROID HORMONE REVERSES AGING-INDUCED MYOCARDIAL FATTY ACID OXIDATION DEFECTS AND IMPROVES THE RESPONSE TO ACUTELY INCREASED AFTERLOAD

    SciTech Connect (OSTI)

    Ledee, Dolena; Portman, Michael A.; Kajimoto, Masaki; Isern, Nancy G.; Olson, Aaron

    2013-06-07T23:59:59.000Z

    Background: Subclinical hypothyroidism occurs during aging in humans and mice and may contribute to development of heart failure. Aging also impairs myocardial fatty acid oxidation, causing increased reliance on flux through pyruvate dehydrogenase (PDH) to maintain function. We hypothesize that the metabolic changes in aged hearts make them less tolerant to acutely increased work and that thyroid hormone reverses these defects. Methods: Studies were performed on young (Young, 4-6 months) and aged (Old, 22-24 months) C57/BL6 mice at standard (50 mmHg) and high afterload (80 mmHg). Another aged group received thyroid hormone for 3 weeks (Old-TH, high afterload only). Function was measured in isolated working hearts along with substrate fractional contributions (Fc) to the citric acid cycle (CAC) using perfusate with 13C labeled lactate, pyruvate, glucose and unlabeled palmitate and insulin. Results: Cardiac function was similar between Young and Old mice at standard afterload. Palmitate Fc was reduced but no individual carbohydrate contributions differed. CAC and individual substrate fluxes decreased in aged. At high afterload, -dP/dT was decreased in Old versus Young. Similar to low afterload, palmitate Fc was decreased in Old. Thyroid hormone reversed aging-induced changes in palmitate Fc and flux while significantly improving cardiac function. Conclusion: The aged heart shows diminished ability to increase cardiac work due to substrate limitations, primarily impaired fatty acid oxidation. The heart accommodates slightly by increasing efficiency through oxidation of carbohydrate substrates. Thyroid hormone supplementation in aged mice significantly improves cardiac function potentially through restoration of fatty acid oxidation.

  9. Investigating fatty acid biosynthesis within the algal chloroplast using Chlamydomonas reinhardtii as a model

    E-Print Network [OSTI]

    Blatti, Jillian L.

    2012-01-01T23:59:59.000Z

    acid biosynthesis in microalgae for sustainable biodiesel. (acid biosynthesis in microalgae for biofuel through protein-acid biosynthesis in microalgae through protein-protein

  10. Volatile fatty acid fermentation of lime-treated bagasse by rumen microorganisms

    E-Print Network [OSTI]

    Lee, Chang-Ming

    1993-01-01T23:59:59.000Z

    untreated bagasse . . . . . . . . Iso-Butyric acid concentrations from untreated bagasse . . . Iso-Valeric acid concentrations from untreated bagasse Total acid concentrations from Ca(OH)2-treated bagasse . . Acetic acid concentrations from Ca(OH)2...(OH)q-treated bagasse . . . . . . . Iso-Butyric acid concentrations from Ca(OH/-treated bagasse . . Iso-Valeric acid concentrations from Ca(OH)q-treated bagasse . . Total acid yields from untreated bagasse . 53 54 55 56 57 59 30 31 32 Acetic acid yields...

  11. Fermentation of pectin and cellulose to short chain fatty acids: a comparative study with humans, baboons, pigs, and rats

    E-Print Network [OSTI]

    Villalba, Leonilde Nonita

    1989-01-01T23:59:59.000Z

    digestibility to the human results. SCFA were measured using gas chromatography. The pH was measured before and after the fermentations. The percent fiber remaining after fermentation was assayed colorimetrically. The greatest interspecies differences were...%I@ W, W, '. '" yW~, t . . . M~~~)~ '1 r FERMENTATION OF PECTIN AND CELLULOSE TO SHORT CHAIN FATTY ACIDS: A COMPARATIVE STUDY WITH HUMANS, BABOONS, PIGS, AND RATS A Thesis by LEONILDE NONITA VILLALBA IL W I Z IJ Z 4 Z 4 2 5 V Z I...

  12. The influence of dietary fat on the fatty acid composition of the egg yolk

    E-Print Network [OSTI]

    Saloma Orozco, Abraham Eduardo

    1966-01-01T23:59:59.000Z

    acids (linolenic and eleostearic) produced an increase in all polyunsaturates having from two to six double bonds. further investigations by the same laboratory (Choudhury and Reiser, 1959) indicate that the inclusion of dietary linoleate at various... number, denoting carbon chain length, is proceeded by a colon, in turn followed by the number of double bonds per molecule. Stearic acid (octadecanoic acid) is thus symbolized by 18:0, oleic acid (octadecenoic acid) by 18:1, arachidonic acid...

  13. Cytoprotective Effects of Dietary Fatty Acids on Oxidative Stress in Hepatocytes

    E-Print Network [OSTI]

    Richardson, Thomas C

    2012-07-11T23:59:59.000Z

    Non-alcoholic fatty liver disease or NAFLD is characterized by the accumulation of fat in the liver, also known as steatosis. This is one of the most common types of liver diseases and can occur in a range of individuals. Once the liver is affected...

  14. Energy densification of biomass-derived organic acids

    DOE Patents [OSTI]

    Wheeler, M. Clayton; van Walsum, G. Peter; Schwartz, Thomas J.; van Heiningen, Adriaan

    2013-01-29T23:59:59.000Z

    A process for upgrading an organic acid includes neutralizing the organic acid to form a salt and thermally decomposing the resulting salt to form an energy densified product. In certain embodiments, the organic acid is levulinic acid. The process may further include upgrading the energy densified product by conversion to alcohol and subsequent dehydration.

  15. Sources and distribution of CuO-derived benzene carboxylic acids in soils and sediments

    E-Print Network [OSTI]

    Long, Bernard

    Sources and distribution of CuO-derived benzene carboxylic acids in soils and sediments Angela F vas- cular plant-derived OC, through the environment. The method produces a suite of benzene

  16. acid derivative functions: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    stem cell (hESC)-derived neurons engineered to be specifically activated by light Fortuna, Miguel A. 22 Functional Determinants in Higher Derivative Lagrangian Theories...

  17. acetic acid derivatives: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    affects the values of the derivatives. Our constructions use randomness extractors and expander graphs. We study our derivatives in a more general setting than Arora et al. In...

  18. Production of volatile fatty acids as a result of bacterial interactions in the cecum

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    that the C. butyricum strain fermented lactose and D-lactic acid to butyric and acetic acids, whereas L-lactic acid was not fermented. The V. alcalescens strain did not ferment lactose and fermented L better than D of lactose (up to 20 %) to the diet increases bacterial fermentation in the large intestine and cecum

  19. Thermosyntropha lipolytica gen. nov., sp. nov., a lipolytic, anaerobic, alkalitolerant, thermophilic bacterium utilizing short- and long-chain fatty acids in syntrophic coculture with a methanogenic archaeum

    SciTech Connect (OSTI)

    Svetlitshnyi, V.; Wiegel, J. [Univ. of Georgia, Athens, GA (United States); Rainey, F. [German Collection of Microorganisms and Cell Cultures, Braunschweig (Germany)

    1996-10-01T23:59:59.000Z

    Three strains of an anaerobic thermophilic organoheterotrophic lipolytic alkalitolerant bacterium, Thermosyntropha lipolytica gen. nov., sp. nov. (type strain JW/VS-264{sup T}; DSM 11003) were isolated from alkaline hot springs of Lake Bogoria (Kenya). The cells were nonmotile, non-spore forming, straight or slightly curved rods. At 60{degrees}C, the pH range for growth determined at 25{degrees}C [pH{sup 25{degrees}C}] was 7.15 to 9.5, with an optimum between 8.1 and 8.9 (pH{sup 60{degrees}C} of 7.6 and 8.1). At a pH{sup 25{degrees}C} of 8.5 temperature range for growth was from 52 to 70{degrees}C, with an optimum between 60 and 66{degrees}C. The shortest doubling time was around 1 h. In pure culture the bacterium grew in a mineral base medium supplemented with yeast extract, tryptone, Casamino Acids, betaine, and crotonate as carbon sources, producing acetate as a major product and constitutively a lipase. During growth in the presence of olive oil, free long-chain fatty acids were accumulated in the medium but the pure culture syntrophic coculture (Methanobacterium strain JW/VS-M29) the lipolytic bacteria grew on triacylglycerols and linear saturated and unsaturated fatty acids with 4 to 18 carbon atoms, but glycerol was not utilized. Fatty acids with even numbers of carbon atoms were degraded to acetate and methane, while from odd-numbered fatty acids 1 mol of propionate per mol of fatty acid was additionally formed. 16S rDNA sequence analysis identified Syntrophospora and Syntrophomonas spp. as closest phylogenetic neighbors.

  20. Evaluation of bone biochemical markers and inflammatory markers in yearlings fed varying ratios of omega-6 and omega-3 polyunsaturated fatty acids

    E-Print Network [OSTI]

    Ross, Trinette Noel

    2009-05-15T23:59:59.000Z

    was similar between groups (P > 0.05). Horses consuming soybean oil (SBO) diet had lower fatty acid profiles (% by weight) of C16:0 and C16:1 (P < 0.05) when compared to horses consuming either corn oil (CO) or menhaden/corn oil (MCO) diets. Though numerically...

  1. Sequential fatty acid analysis of a peat core covering the last two millennia (Tritrivakely lake, Madagascar): diagenesis appraisal and consequences for palaeoenvironmental

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    1 Sequential fatty acid analysis of a peat core covering the last two millennia (Tritrivakely lake and petrographical work [2], we applied such an approach to the analysis of a peat core section to get additional (saturated and unsaturated n-FAs, plus i-C16) at the surface of the peat sequence and low amounts

  2. Preparation of Propylene Glycol Fatty Acid Ester or Other Glycol, or Polyol

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level:Energy: Grid Integration Redefining What's Possible forPortsmouth/Paducah ProjectPRE-AWARD ACCOUNTINGQuantitative hiRXand Aeroacoustic*Fatty

  3. Low-Emissions Burner Technology using Biomass-Derived Liquid...

    Broader source: Energy.gov (indexed) [DOE]

    This factsheet describes a project that developed fuel-flexible, low-emissions burner technology capable of using biomass-derived liquid fuels, such as glycerin or fatty acids, as...

  4. Inhibitions of ascorbate fatty acid derivatives on three rabbit muscle glycolytic enzymes

    E-Print Network [OSTI]

    Pham, Duyen-Anh

    2011-01-01T23:59:59.000Z

    PET), primary and secondary metastatic cancer cells exhibit upregulated aerobic glycolysis and result in increased glucose consumption [

  5. Comparative investigation of volatile fatty acids, soluble glucides and cellulase enzymes activity

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    and Animal Nutrition, 8113, Balotesti, Romania Following a comparative study of the ruminal fermentation in buffaloes for both diets. The molar proportion of acetic acid was significantly higher in buffaloes

  6. Improving fatty acids production by engineering dynamic pathway regulation and metabolic control

    E-Print Network [OSTI]

    Stephanopoulos, Gregory

    Global energy demand and environmental concerns have stimulated increasing efforts to produce carbon-neutral fuels directly from renewable resources. Microbially derived aliphatic hydrocarbons, the petroleum-replica fuels, ...

  7. Constructing and engineering fatty acid metabolic pathways for the production of fuels and chemicals

    E-Print Network [OSTI]

    Steen, Eric James

    2010-01-01T23:59:59.000Z

    motivated production of renewable, biomass-derived fuels andconversion of renewable feedstocks, such as biomass-derivedrenewable energy is the production of these compounds directly from cellulosic plant biomass.

  8. acid derivative dynamically: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    in addition to the usual ... Liu, Jun 2003-09-25 2 Realistic Derivation of Heisenberg Dynamics Quantum Physics (arXiv) Summary: Einstein conjectured long ago that much of quantum...

  9. Fatty acid composition in the mature milk of Bolivian forager-horticulturalists: controlled comparisons with a

    E-Print Network [OSTI]

    Gurven, Michael

    comparisons with a US samplemcn_412 404..418 Melanie A. Martin*, William D. Lassek , Steven J.C. Gaulin, arachidonic acid, breast milk, infant and child nutrition. Correspondence: Melanie A. Martin, Integrative affecting the percent- ages of many specific FAs, including DHA, in milk (Jensen 1999; Innis 2007b). FAs

  10. A Direct, Biomass-Based Synthesis of Benzoic Acid: Formic Acid-Mediated Deoxygenation of the Glucose-Derived Materials Quinic Acid and Shikimic Acid

    SciTech Connect (OSTI)

    Arceo, Elena; Ellman, Jonathan; Bergman, Robert

    2010-05-03T23:59:59.000Z

    An alternative biomass-based route to benzoic acid from the renewable starting materials quinic acid and shikimic acid is described. Benzoic acid is obtained selectively using a highly efficient, one-step formic acid-mediated deoxygenation method.

  11. Technological and economic potential of poly(lactic acid) and lactic acid derivatives

    SciTech Connect (OSTI)

    Datta, R.; Tsai, S.P.; Bonsignore, P.; Moon, S.H.; Frank, J.R.

    1993-10-01T23:59:59.000Z

    Lactic acid has been an intermediate-volume specialty chemical (world production {approximately}40,000 tons/yr) used in a wide range of food processing and industrial applications. lactic acid h,as the potential of becoming a very large volume, commodity-chemical intermediate produced from renewable carbohydrates for use as feedstocks for biodegradable polymers, oxygenated chemicals, plant growth regulators, environmentally friendly ``green`` solvents, and specially chemical intermediates. In the past, efficient and economical technologies for the recovery and purification of lactic acid from crude fermentation broths and the conversion of tactic acid to the chemical or polymer intermediates had been the key technology impediments and main process cost centers. The development and deployment of novel separations technologies, such as electrodialysis (ED) with bipolar membranes, extractive distillations integrated with fermentation, and chemical conversion, can enable low-cost production with continuous processes in large-scale operations. The use of bipolar ED can virtually eliminate the salt or gypsum waste produced in the current lactic acid processes. In this paper, the recent technical advances in tactic and polylactic acid processes are discussed. The economic potential and manufacturing cost estimates of several products and process options are presented. The technical accomplishments at Argonne National Laboratory (ANL) and the future directions of this program at ANL are discussed.

  12. Nickel(II) and copper(II) complexes with humic acid anions and their derivatives

    SciTech Connect (OSTI)

    Ryabova, I.N. [Kazakh Academy of Science, Karaganda (Kazakhstan)

    2008-01-15T23:59:59.000Z

    Complexation of Ni(II) and Cu(II) in aqueous solutions with anions of humic acids, extracted from naturally oxidized coal, and with their hydroxymethyl derivatives is studied spectrophotometrically and potentiometrically. The complexation stoichiometry and the stability constants of the complexes are determined.

  13. Scaleable production and separation of fermentation-derived acetic acid. Final CRADA report.

    SciTech Connect (OSTI)

    Snyder, S. W.; Energy Systems

    2010-02-08T23:59:59.000Z

    Half of U.S. acetic acid production is used in manufacturing vinyl acetate monomer (VAM) and is economical only in very large production plants. Nearly 80% of the VAM is produced by methanol carbonylation, which requires high temperatures and exotic construction materials and is energy intensive. Fermentation-derived acetic acid production allows for small-scale production at low temperatures, significantly reducing the energy requirement of the process. The goal of the project is to develop a scaleable production and separation process for fermentation-derived acetic acid. Synthesis gas (syngas) will be fermented to acetic acid, and the fermentation broth will be continuously neutralized with ammonia. The acetic acid product will be recovered from the ammonium acid broth using vapor-based membrane separation technology. The process is summarized in Figure 1. The two technical challenges to success are selecting and developing (1) microbial strains that efficiently ferment syngas to acetic acid in high salt environments and (2) membranes that efficiently separate ammonia from the acetic acid/water mixture and are stable at high enough temperature to facilitate high thermal cracking of the ammonium acetate salt. Fermentation - Microbial strains were procured from a variety of public culture collections (Table 1). Strains were incubated and grown in the presence of the ammonium acetate product and the fastest growing cultures were selected and incubated at higher product concentrations. An example of the performance of a selected culture is shown in Figure 2. Separations - Several membranes were considered. Testing was performed on a new product line produced by Sulzer Chemtech (Germany). These are tubular ceramic membranes with weak acid functionality (see Figure 3). The following results were observed: (1) The membranes were relatively fragile in a laboratory setting; (2) Thermally stable {at} 130 C in hot organic acids; (3) Acetic acid rejection > 99%; and (4) Moderate ammonia flux. The advantages of producing acetic acid by fermentation include its appropriateness for small-scale production, lower cost feedstocks, low energy membrane-based purification, and lower temperature and pressure requirements. Potential energy savings of using fermentation are estimated to be approximately 14 trillion Btu by 2020 from a reduction in natural gas use. Decreased transportation needs with regional plants will eliminate approximately 200 million gallons of diesel consumption, for combined savings of 45 trillion Btu. If the fermentation process captures new acetic acid production, savings could include an additional 5 trillion Btu from production and 7 trillion Btu from transportation energy.

  14. The mitochondrial fatty acid synthesis (mtFASII) pathway is capable of mediating nuclear-mitochondrial cross talk through the PPAR system of transcriptional activation

    SciTech Connect (OSTI)

    Parl, Angelika; Mitchell, Sabrina L.; Clay, Hayley B.; Reiss, Sara; Li, Zhen; Murdock, Deborah G., E-mail: deborah.murdock@vanderbilt.edu

    2013-11-15T23:59:59.000Z

    Highlights: •The function of the mitochondria fatty acid synthesis pathway is partially unknown. •Overexpression of the pathway causes transcriptional activation through PPARs. •Knock down of the pathway attenuates that activation. •The last enzyme in the pathway regulates its own transcription. •Products of the mtFASII pathway are able to drive nuclear transcription. -- Abstract: Mammalian cells contain two fatty acid synthesis pathways, the cytosolic FASI pathway, and the mitochondrial FASII pathway. The selection behind the conservation of the mitochondrial pathway is not completely understood, given the presence of the cytosolic FAS pathway. In this study, we show through heterologous gene reporter systems and PCR-based arrays that overexpression of MECR, the last step in the mtFASII pathway, causes modulation of gene expression through the PPAR pathway. Electromobility shift assays (EMSAs) demonstrate that overexpression of MECR causes increased binding of PPARs to DNA, while cell fractionation and imaging studies show that MECR remains localized to the mitochondria. Interestingly, knock down of the mtFASII pathway lessens the effect of MECR on this transcriptional modulation. Our data are most consistent with MECR-mediated transcriptional activation through products of the mtFASII pathway, although we cannot rule out MECR acting as a coactivator. Further investigation into the physiological relevance of this communication will be necessary to better understand some of the phenotypic consequences of deficits in this pathway observed in animal models and human disease.

  15. THE COMPETITION BETWEEN METHYLMERCURY RISKS AND OMEGA-3 POLYUNSATURATED FATTY ACID BENEFITS: A REVIEW OF CONFLICTING EVIDENCE ON FISH CONSUMPTION AND CARDIOVASCULAR HEALTH.

    SciTech Connect (OSTI)

    LIPFERT, F.W.; SULLIVAN, T.M.

    2006-10-31T23:59:59.000Z

    The health concerns of methylmercury (MeHg) contamination of seafood have recently been extended to include cardiovascular effects, especially premature mortality. Although the fatty acids (fish oils) found in most species are thought to confer a wide range of health benefits, especially to the cardiovascular system, some epidemiological studies have suggested that such benefits may be offset by adverse effects of MeHg. This comprehensive review is based on searches of the NIH MEDLINE database and compares and contrasts 145 published studies involving cardiovascular effects and exposures to mercury and other fish contaminants, intake of fatty acids including dietary supplements of fish oils, and rates of seafood consumption. Since few of these studies include adequate simultaneous measurements of all of these potential predictor variables, we summarized their effects separately, across the available studies of each, and then drew conclusions based on the aggregated findings. It is important to realize that studies of seafood consumption encompass the net effects of all of these predictor variables, but that seafood intake studies are rarely supported by human biomarker measurements that reflect the actual uptake of harmful as well as beneficial fish ingredients. As a result, exposure measurement error is an issue when comparing studies and predictor variables. It is also possible that the observed benefits of eating fish may relate more to the characteristics of the consumers than to those of the fish. We found the evidence for adverse cardiovascular effects of MeHg to be sparse and unconvincing. Studies of cardiovascular mortality show net benefits, and the findings of adverse effects are mainly limited to studies Finland at high mercury exposure levels. By contrast, a very consistent picture of beneficial effects is seen for fatty acids, after recognizing the effects of exposure uncertainties and the presence of threshold effects. Studies based on measured biomarker levels are seen to be the most reliable and present a convincing picture of strong beneficial effects, especially for those causes of death involving cardiac arrhythmia. This conclusion also extends to studies of fish-oil supplementation. Studies based on fish consumption show mainly benefits from increased consumption. This finding is supported by an ecological study at the national population level, for which the lifestyle effects that might be correlated with fish consumption within a given population would be expected to ''average out'' across nations. Finally, the net survival benefits resulting from eating fish are consistent with studies involving complete diets, although benefits are also seen to accrue from reduced consumption of red meat and saturated fats.

  16. A determination of the heats of combustion of the five-carbon fatty acids and their methyl and ethyl esters

    E-Print Network [OSTI]

    Gilby, Ralph F

    1952-01-01T23:59:59.000Z

    ; g7;P~. X% FATTI ACXDS ND TAX'. K~~~. Ah. ' RKg', Ei~K'~ P~ FuXVQJ~ ef %he 1&aydgewetie fea %he Twine of ~ of . "-elmer F~ F QQXly xy, ay~ Oe esther w9atew to thank Lh ~ C+ K ~ eeet W 9 H~ Vetoes ebs 45xoe~ @de esesaveh The ~ also ~ to thsnk N... of preparation had to be used? Tbongh sane diff ioalt and tins oonsnaing than sxxidationx the prsparatdon through the Orignard synthesis prosed quite satisfaotory? Isobntyl ~ prepared frcxa the aloohol was converted to isobutgrl nayvvxtuxs bread, de whioh...

  17. A determination of the heats of combustion of the five-carbon fatty acids and their methyl and ethyl esters 

    E-Print Network [OSTI]

    Gilby, Ralph F

    1952-01-01T23:59:59.000Z

    ; g7;P~. X% FATTI ACXDS ND TAX'. K~~~. Ah. ' RKg', Ei~K'~ P~ FuXVQJ~ ef %he 1&aydgewetie fea %he Twine of ~ of . "-elmer F~ F QQXly xy, ay~ Oe esther w9atew to thank Lh ~ C+ K ~ eeet W 9 H~ Vetoes ebs 45xoe~ @de esesaveh The ~ also ~ to thsnk N... of preparation had to be used? Tbongh sane diff ioalt and tins oonsnaing than sxxidationx the prsparatdon through the Orignard synthesis prosed quite satisfaotory? Isobntyl ~ prepared frcxa the aloohol was converted to isobutgrl nayvvxtuxs bread, de whioh...

  18. Fatty acid composition of retail cuts trimmed to different external fat levels from choice, select, and standard beef grade carcasses

    E-Print Network [OSTI]

    Sullivan, Dana Renee

    1988-01-01T23:59:59.000Z

    distillation system. Distilled water (50 ml) was added to each tube. A flask containing 25 ml boric acid was placed on the distillation platform so that the condenser tube was below the surface of the boric acid solution. To generate steam for distillation..., 40$ NaOH was dispensed into the digestion tube. When distillation was completed, the boric acid solution was titrated with 0. 1 N sulfuric acid using a Tecator titr ation apparatus . Endpoint was indicated by a neutral gray color. The amount...

  19. acid solutions part: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    m Popovych, Roman 71 Z .Comparative Biochemistry and Physiology Part B 128 2001 445 450 Purification and characterization of the fatty acid Chemistry Websites Summary: fatty acid...

  20. acid synthase activity: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    use Rippe, Karsten 53 Z .Comparative Biochemistry and Physiology Part B 128 2001 445 450 Purification and characterization of the fatty acid Chemistry Websites Summary: fatty acid...

  1. A Direct, Biomass-Based Synthesis of Benzoic Acid: Formic Acid-Mediated Deoxygenation of the Glucose-Derived Materials Quinic Acid and Shikimic Acid

    E-Print Network [OSTI]

    2010-01-01T23:59:59.000Z

    R.G.B and J.A.E. ). Keywords: biomass · carboxylic acids ·10.1002/cssc.201000111 A Direct, Biomass-Based Synthesis ofaro- matic compounds from biomass resources could provide a

  2. Omega-3 polyunsaturated fatty acid has an anti-oxidant effect via the Nrf-2/HO-1 pathway in 3T3-L1 adipocytes

    SciTech Connect (OSTI)

    Kusunoki, Chisato, E-mail: yosizaki@belle.shiga-med.ac.jp [Department of Medicine, Shiga University of Medical Science, Seta Tsukinowa-Cho, Otsu, Shiga 520-2192 (Japan)] [Department of Medicine, Shiga University of Medical Science, Seta Tsukinowa-Cho, Otsu, Shiga 520-2192 (Japan); Yang, Liu; Yoshizaki, Takeshi; Nakagawa, Fumiyuki; Ishikado, Atsushi; Kondo, Motoyuki; Morino, Katsutaro; Sekine, Osamu; Ugi, Satoshi [Department of Medicine, Shiga University of Medical Science, Seta Tsukinowa-Cho, Otsu, Shiga 520-2192 (Japan)] [Department of Medicine, Shiga University of Medical Science, Seta Tsukinowa-Cho, Otsu, Shiga 520-2192 (Japan); Nishio, Yoshihiko [Division of Diabetes, Metabolism and Endocrinology, Department of Graduate School of Medical and Dental Sciences, Kagoshima University, 8-35-1 Sakuragaoka, Kagoshima 890-8544 (Japan)] [Division of Diabetes, Metabolism and Endocrinology, Department of Graduate School of Medical and Dental Sciences, Kagoshima University, 8-35-1 Sakuragaoka, Kagoshima 890-8544 (Japan); Kashiwagi, Atsunori; Maegawa, Hiroshi [Department of Medicine, Shiga University of Medical Science, Seta Tsukinowa-Cho, Otsu, Shiga 520-2192 (Japan)] [Department of Medicine, Shiga University of Medical Science, Seta Tsukinowa-Cho, Otsu, Shiga 520-2192 (Japan)

    2013-01-04T23:59:59.000Z

    Highlights: Black-Right-Pointing-Pointer Omega-3 PUFA has a direct anti-oxidant effect in adipocytes. Black-Right-Pointing-Pointer EPA and DHA induce HO-1 expression in 3T3-L1 adipocytes. Black-Right-Pointing-Pointer Omega-3 PUFA and its end-product, 4-HHE, activates the Nrf-2/HO-1 pathway. Black-Right-Pointing-Pointer Omega-3 PUFA protects against oxidative stress-induced cytotoxicity. -- Abstract: Oxidative stress is produced in adipose tissue of obese subjects and has been associated with obesity-related disorders. Recent studies have shown that omega-3 polyunsaturated fatty acid ({omega}3-PUFA) has beneficial effects in preventing atherosclerotic diseases and insulin resistance in adipose tissue. However, the role of {omega}3-PUFA on adipocytes has not been elucidated. In this study, 3T3-L1 adipocytes were treated with {omega}3-PUFA and its metabolites, eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), or 4-hydroxy hexenal (4-HHE). {omega}3-PUFA and its metabolites dose-dependently increased mRNA and protein levels of the anti-oxidative enzyme, heme oxygenase-1 (HO-1); whereas no changes in the well-known anti-oxidant molecules, superoxide dismutase, catalase, and glutathione peroxidase, were observed. Knockdown of nuclear factor erythroid 2-related factor 2 (Nrf-2) significantly reduced EPA, DHA or 4-HHE-induced HO-1 mRNA and protein expression. Also, pretreatment with {omega}3-PUFA prevented H{sub 2}O{sub 2}-induced cytotoxicity in a HO-1 dependent manner. In conclusion, treatment with EPA and DHA induced HO-1 through the activation of Nrf-2 and prevented oxidative stress in 3T3-L1 adipocytes. This anti-oxidant defense may be of high therapeutic value for clinical conditions associated with systemic oxidative stress.

  3. Saturated fatty acid palmitate induces extracellular release of histone H3: A possible mechanistic basis for high-fat diet-induced inflammation and thrombosis

    SciTech Connect (OSTI)

    Shrestha, Chandan [Department of Systems Biology in Thromboregulation, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima (Japan) [Department of Systems Biology in Thromboregulation, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima (Japan); Department of Laboratory and Vascular Medicine, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima (Japan); Ito, Takashi [Department of Systems Biology in Thromboregulation, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima (Japan)] [Department of Systems Biology in Thromboregulation, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima (Japan); Kawahara, Ko-ichi [Department of Biomedical Engineering, Osaka Institute of Technology, Osaka (Japan)] [Department of Biomedical Engineering, Osaka Institute of Technology, Osaka (Japan); Shrestha, Binita; Yamakuchi, Munekazu; Hashiguchi, Teruto [Department of Laboratory and Vascular Medicine, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima (Japan)] [Department of Laboratory and Vascular Medicine, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima (Japan); Maruyama, Ikuro, E-mail: rinken@m3.kufm.kagoshima-u.ac.jp [Department of Systems Biology in Thromboregulation, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima (Japan)] [Department of Systems Biology in Thromboregulation, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima (Japan)

    2013-08-09T23:59:59.000Z

    Highlights: •High-fat diet feeding and palmitate induces the release of nuclear protein histone H3. •ROS production and JNK signaling mediates the release of histone H3. •Extracellular histones induces proinflammatory and procoagulant response. -- Abstract: Chronic low-grade inflammation is a key contributor to high-fat diet (HFD)-related diseases, such as type 2 diabetes, non-alcoholic steatohepatitis, and atherosclerosis. The inflammation is characterized by infiltration of inflammatory cells, particularly macrophages, into obese adipose tissue. However, the molecular mechanisms by which a HFD induces low-grade inflammation are poorly understood. Here, we show that histone H3, a major protein component of chromatin, is released into the extracellular space when mice are fed a HFD or macrophages are stimulated with the saturated fatty acid palmitate. In a murine macrophage cell line, RAW 264.7, palmitate activated reactive oxygen species (ROS) production and JNK signaling. Inhibitors of these pathways dampened palmitate-induced histone H3 release, suggesting that the extracellular release of histone H3 was mediated, in part, through ROS and JNK signaling. Extracellular histone activated endothelial cells toexpress the adhesion molecules ICAM-1 and VCAM-1 and the procoagulant molecule tissue factor, which are known to contribute to inflammatory cell recruitment and thrombosis. These results suggest the possible contribution of extracellular histone to the pathogenesis of HFD-induced inflammation and thrombosis.

  4. Quercetin and Chlorogenic Acid Mitigate DSS-Induced Changes in Expression of Select Pro-Inflammatory Cytokines and Short Chain Fatty Acid Transporter Genes

    E-Print Network [OSTI]

    Piefer, Leigh

    2012-10-19T23:59:59.000Z

    oxide synthase LPS lipopolysaccharide LS least squares MCT-1 monocarboxylate transporter-1 MyD88 myeloid differentiation factor-88 NF-?B nuclear transcription factor kappa B PBS phosphate buffered solution PFA paraformaldehyde RT-PCR reverse... animals (acetic acid, pMCT-1 and SLC5A8 mRNA levels show that DSS significantly...

  5. Fatty Acid-Producing Microbes for Generating Medium- and Long-Chain Hydrocarbons: Inventors: Brian Pfleger, Rebecca Lennen

    Energy Innovation Portal (Marketing Summaries) [EERE]

    2011-06-01T23:59:59.000Z

    New, renewable sources of transportation fuel are needed to meet continuing demand. While the main focus has been on biomass-derived gasoline alternatives such as ethanol and other short-chain alcohols, distillates with higher energy density such as diesel and jet fuel are required for the heavy transportation sector. Biodiesel is a biodegradable, clean-burning combustible fuel made of medium- to long-chain hydrocarbons that can be used in most internal combustion diesel engines. Current...

  6. Oligonucleoside alkyl or arylphosphonate derivatives capable of crosslinking with or cleaving nucleic acids

    DOE Patents [OSTI]

    Miller, Paul S. (Baltimore, MD); Ts'o, Paul O.P. (Lutherville, MD)

    1999-06-15T23:59:59.000Z

    A composition for inactivating a target nucleic acid which comprises an oligonucleoside alkyl or arylphosphonate analogue which is complementary to the sequence of the target nucleic acid and includes a functional group which reacts with the target nucleic acid to render the target nucleic acid inactive or nonfunctional.

  7. Oligonucleoside alkyl or arylphosphonate derivatives capable of crosslinking with or cleaving nucleic acids

    DOE Patents [OSTI]

    Miller, P.S.; Ts'o, P.O.P.

    1999-06-15T23:59:59.000Z

    A composition for inactivating a target nucleic acid which comprises an oligonucleoside alkyl or arylphosphonate analogue which is complementary to the sequence of the target nucleic acid is provided. It includes a functional group which reacts with the target nucleic acid to render the target nucleic acid inactive or nonfunctional. 16 figs.

  8. A novel, easily synthesized, anhydrous derivative of phosphoric acid for use with electrolyte in phosphoric acid-based fuel cells.

    E-Print Network [OSTI]

    Angell, C. Austen

    ] such as polybenzimidazole, into a very promising and cost-competitive alternative[4] to the Nafion fuel cell[5, 6 in phosphoric acid-based fuel cells. Younes Ansari, Telpriore Tucker, and C. Austen Angell* Dept. of Chemistry as a fuel cell electrolyte, by designing a variant of the molecular acid that has increased temperature

  9. E-Print Network 3.0 - acid derivative protein Sample Search Results

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    North Carolina State University Collection: Biology and Medicine 5 Cotranslational protein folding with L-systems Gemma B. Danks1,2 Summary: derivation steps. The axiom in our...

  10. Nucleic Acid Encoding A Lectin-Derived Progenitor Cell Preservation Factor

    DOE Patents [OSTI]

    Colucci, M. Gabriella (Dugenta, IT); Chrispeels, Maarten J. (La Jolla, CA); Moore, Jeffrey G. (New York, NY)

    2001-10-30T23:59:59.000Z

    The invention relates to an isolated nucleic acid molecule that encodes a protein that is effective to preserve progenitor cells, such as hematopoietic progenitor cells. The nucleic acid comprises a sequence defined by SEQ ID NO:1, a homolog thereof, or a fragment thereof. The encoded protein has an amino acid sequence that comprises a sequence defined by SEQ ID NO:2, a homolog thereof, or a fragment thereof that contains an amino acid sequence TNNVLQVT. Methods of using the encoded protein for preserving progenitor cells in vitro, ex vivo, and in vivo are also described. The invention, therefore, include methods such as myeloablation therapies for cancer treatment wherein myeloid reconstitution is facilitated by means of the specified protein. Other therapeutic utilities are also enabled through the invention, for example, expanding progenitor cell populations ex vivo to increase chances of engraftation, improving conditions for transporting and storing progenitor cells, and facilitating gene therapy to treat and cure a broad range of life-threatening hematologic diseases.

  11. Effects of the addition of water-filled bladders or volatile fatty acids to the rumen on the feeding behaviour in lactating dairy cows

    E-Print Network [OSTI]

    Boyer, Edmond

    tests (rumen bladders and VFA infusion) were carried out at 4, 9, 15 and 35 wk after calving in 4) was compared to a bladder-free control. In the second test, a con- tinuous infusion of 20.3 MJ/d volatile fatty- men was compared to a control infusion (7 g NaCl, 3.7 g KCI and 2.4 ml HCI 2 N perI water

  12. acid mediates brain-derived: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    mediates brain-derived First Page Previous Page 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 Next Page Last Page Topic Index 1 DevelopmentPlasticityRepair...

  13. Analysis of potential combustion source impacts on acid deposition using an independently derived inventory. Volume I

    SciTech Connect (OSTI)

    Not Available

    1983-12-01T23:59:59.000Z

    This project had three major objectives. The first objective was to develop a fossil fuel combustion source inventory (NO/sub x/, SO/sub x/, and hydrocarbon emissions) that would be relatively easy to use and update for analyzing the impact of combustion emissions on acid deposition in the eastern United States. The second objective of the project was to use the inventory data as a basis for selection of a number of areas that, by virtue of their importance in the acid rain issue, could be further studied to assess the impact of local and intraregional combustion sources. The third objective was to conduct an analysis of wet deposition monitoring data in the areas under study, along with pertinent physical characteristics, meteorological conditions, and emission patterns of these areas, to investigate probable relationships between local and intraregional combustion sources and the deposition of acidic material. The combustion source emissions inventory has been developed for the eastern United States. It characterizes all important area sources and point sources on a county-by-county basis. Its design provides flexibility and simplicity and makes it uniquely useful in overall analysis of emission patterns in the eastern United States. Three regions with basically different emission patterns have been identified and characterized. The statistical analysis of wet deposition monitoring data in conjunction with emission patterns, wind direction, and topography has produced consistent results for each study area and has demonstrated that the wet deposition in each area reflects the characteristics of the localized area around the monitoring sites (typically 50 to 150 miles). 8 references, 28 figures, 39 tables.

  14. acid methyl esters: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    3 (> 170 o C). Each fraction was analyzed composition of fatty acid ethyl esters using gas chromatography (GC). The result showed that the yield medium chain fatty acid ethyl...

  15. acidic antigenic fractions: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    3 (> 170 o C). Each fraction was analyzed composition of fatty acid ethyl esters using gas chromatography (GC). The result showed that the yield medium chain fatty acid ethyl...

  16. acid ester homologs: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    3 (> 170 o C). Each fraction was analyzed composition of fatty acid ethyl esters using gas chromatography (GC). The result showed that the yield medium chain fatty acid ethyl...

  17. acid methyl ester: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    3 (> 170 o C). Each fraction was analyzed composition of fatty acid ethyl esters using gas chromatography (GC). The result showed that the yield medium chain fatty acid ethyl...

  18. acid binding proteins: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The adipocyte fatty acid-binding protein aP2 regulates systemic glucose (more) Shum, Bennett Oh Vic 2007-01-01 2 Fatty acid-binding protein in bovine skeletal muscle Texas A&M...

  19. acid base regulation: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The adipocyte fatty acid-binding protein aP2 regulates systemic glucose (more) Shum, Bennett Oh Vic 2007-01-01 3 Stabilized epoxygenated fatty acids regulate inflammation, pain,...

  20. acid dopac binds: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The adipocyte fatty acid-binding protein aP2 regulates systemic glucose (more) Shum, Bennett Oh Vic 2007-01-01 2 Fatty acid-binding protein in bovine skeletal muscle Texas A&M...

  1. Structural determination of triclosan derivatives as inhibitors of Plasmodium falciparum enoyl reductase (PfENR)

    E-Print Network [OSTI]

    Lucumi Moreno, Edinson

    2007-04-25T23:59:59.000Z

    -malaria drugs is necessary in modern days. Recent studies have shown that the parasite synthesizes fatty acids using a fatty acid synthase type II (FAS-II) instead of a type-I fatty acid synthase (FAS-I) that is present in other eukaryotes. Plasmodium falciparum...

  2. EFFECT OF HISTAMINE ON FREE FATTY ACIDS IN RUMINANTS' A. R. 0., The Vo%ani Center, P. 0. B. 6, Bet Dagan, Israel

    E-Print Network [OSTI]

    Boyer, Edmond

    deals with the effect of omasal or intravenous infusions of hista- mine on the level of plasma free.5g histamine-2HCI and 16 ml formic acid were infused into the oma- sum by means of a cannula inserted assay of FFA. In all experi- ments blood was collected immediately before the histamine infusions

  3. Dietary polyunsaturated fatty acids modify plasma lipids and red blood cell membrane composition but do not induce ?6 desaturase mediated conversions in the domestic feline

    E-Print Network [OSTI]

    McClure, Melena Kathleen

    2009-05-15T23:59:59.000Z

    diets consisted of a commercial diet, supplemented with 8g oil/100g kibble. Oil supplements and subsequent diets were: high-oleic sunflower (H diet) with 82% oleic acid (18:1n-9), Menhaden fish (M diet) with LCn-3FA, and safflower (S diet) with 75% 18:2n...

  4. Identification of 6-octadecynoic acid from a methanol extract of Marrubium vulgare L. as a peroxisome proliferator-activated receptor ? agonist

    SciTech Connect (OSTI)

    Ohtera, Anna; Miyamae, Yusaku; Nakai, Naomi [Graduate School of Biostudies, Kyoto University, Kyoto 606-8502 (Japan)] [Graduate School of Biostudies, Kyoto University, Kyoto 606-8502 (Japan); Kawachi, Atsushi; Kawada, Kiyokazu; Han, Junkyu; Isoda, Hiroko [Alliance for Research on North Africa (ARENA), University of Tsukuba, Ibaraki 305-8572 (Japan) [Alliance for Research on North Africa (ARENA), University of Tsukuba, Ibaraki 305-8572 (Japan); Faculty of Life and Environment, University of Tsukuba, Ibaraki 305-8572 (Japan); Neffati, Mohamed [Arid Zone Research Institute (IRA), Médenine 4119 (Tunisia)] [Arid Zone Research Institute (IRA), Médenine 4119 (Tunisia); Akita, Toru; Maejima, Kazuhiro [Nippon Shinyaku CO., LTD., Kyoto 601-8550 (Japan)] [Nippon Shinyaku CO., LTD., Kyoto 601-8550 (Japan); Masuda, Seiji; Kambe, Taiho [Graduate School of Biostudies, Kyoto University, Kyoto 606-8502 (Japan)] [Graduate School of Biostudies, Kyoto University, Kyoto 606-8502 (Japan); Mori, Naoki; Irie, Kazuhiro [Graduate School of Agriculture, Kyoto University, Kyoto 606-8502 (Japan)] [Graduate School of Agriculture, Kyoto University, Kyoto 606-8502 (Japan); Nagao, Masaya, E-mail: mnagao@kais.kyoto-u.ac.jp [Graduate School of Biostudies, Kyoto University, Kyoto 606-8502 (Japan)] [Graduate School of Biostudies, Kyoto University, Kyoto 606-8502 (Japan)

    2013-10-18T23:59:59.000Z

    Highlights: •6-ODA, a rare fatty acid with a triple bond, was identified from Marrubium vulgare. •6-ODA was synthesized from petroselinic acid as a starting material. •6-ODA stimulated lipid accumulation in HSC-T6 and 3T3-L1 cells. •The first report of a fatty acid with a triple bond functioning as a PPAR? agonist. •This study sheds light on novel functions of a fatty acid with a triple bond. -- Abstract: 6-Octadecynoic acid (6-ODA), a fatty acid with a triple bond, was identified in the methanol extract of Marrubium vulgare L. as an agonist of peroxisome proliferator-activated receptor ? (PPAR?). Fibrogenesis caused by hepatic stellate cells is inhibited by PPAR? whose ligands are clinically used for the treatment of diabetes. Plant extracts of Marrubium vulgare L., were screened for activity to inhibit fibrosis in the hepatic stellate cell line HSC-T6 using Oil Red-O staining, which detects lipids that typically accumulate in quiescent hepatic stellate cells. A methanol extract with activity to stimulate accumulation of lipids was obtained. This extract was found to have PPAR? agonist activity using a luciferase reporter assay. After purification using several chromatographic methods, 6-ODA, a fatty acid with a triple bond, was identified as a candidate of PPAR? agonist. Synthesized 6-ODA and its derivative 9-octadecynoic acid (9-ODA), which both have a triple bond but in different positions, activated PPAR? in a luciferase reporter assay and increased lipid accumulation in 3T3-L1 adipocytes in a PPAR?-dependent manner. There is little information about the biological activity of fatty acids with a triple bond, and to our knowledge, this is the first report that 6-ODA and 9-ODA function as PPAR? agonists.

  5. acid-binding protein genes: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The adipocyte fatty acid-binding protein aP2 regulates systemic glucose (more) Shum, Bennett Oh Vic 2007-01-01 2 Fatty acid-binding protein in bovine skeletal muscle Texas A&M...

  6. acid-binding protein ii: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The adipocyte fatty acid-binding protein aP2 regulates systemic glucose (more) Shum, Bennett Oh Vic 2007-01-01 2 Fatty acid-binding protein in bovine skeletal muscle Texas A&M...

  7. acid-binding immunoglobulin-like lectin-6: Topics by E-print...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The adipocyte fatty acid-binding protein aP2 regulates systemic glucose (more) Shum, Bennett Oh Vic 2007-01-01 2 Fatty acid-binding protein in bovine skeletal muscle Texas A&M...

  8. The Fatty Acid Content of Ocean Water

    E-Print Network [OSTI]

    Slowey, James Frank

    1960-01-01T23:59:59.000Z

    acetate sea water ethyl acetate 28, 620 226, 750 13, 780 1Z, 500 9, 540 5, 500 89 5-1/ 2 Run 2 sea water ethyl acetate sea water ethyl acetate sea water ethyl acetate 26, 500 20Z, 500 13, 780 12, 250 8, 480 3, 750 11-1/ 2 88-1/ 2...'ters)(CIpCIpCI2C14 14(1=) 16 16(1 =) 18 18(1 =) 18(2 =) Number of Carbon Atoms t. % Wei ht of Esters mg. /I. 10 300 900 1900 0 6Q 20 4 3Q 12 11 7 35 94 0 0 0 7 6 42 15 0 22 16 Bg 14 9 0 0 0 0 0. 5 0. 4 0. 5 0. 3 23 FIGURE 2 SEPARATION...

  9. acid degradation products: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFRICAN DWARF SHEEP CiteSeer Summary: This study describes the effect of intraruminal infusion of different proportions of palmitic (saturated fatty acid) and linolenic...

  10. acid sunflower oil: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    lipid metabolism in an atherogenic resistant species, effects of n-3 fatty acid rich fish oil on canine lipoproteins and lecithin:cholesterol acyltransferase (LCAT) activities...

  11. acid production plant: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Summary: . - ,, ,,, , -,, . 12;Volatile fatty acids (VIA), produced mainly from fermentation of carbohydrates in the ration of carbohydrates and the production of VFA have not...

  12. Method for production of petroselinic acid and OMEGA12 hexadecanoic acid in transgenic plants

    DOE Patents [OSTI]

    Ohlrogge, John B. (Okemos, MI); Cahoon, Edgar B. (Lansing, MI); Shanklin, John (Upton, NY); Somerville, Christopher R. (Okemos, MI)

    1995-01-01T23:59:59.000Z

    The present invention relates to a process for producing lipids containing the fatty acid petroselinic acid in plants. The production of petroselinic acid is accomplished by genetically transforming plants which do not normally accumulate petroselinic acid with a gene for a .omega.12 desaturase from another species which does normally accumulate petroselinic acid.

  13. Method for production of petroselinic acid and OMEGA12 hexadecanoic acid in transgenic plants

    DOE Patents [OSTI]

    Ohlrogge, J.B.; Cahoon, E.B.; Shanklin, J.; Somerville, C.R.

    1995-07-04T23:59:59.000Z

    The present invention relates to a process for producing lipids containing the fatty acid, petroselinic acid, in plants. The production of petroselinic acid is accomplished by genetically transforming plants which do not normally accumulate petroselinic acid with a gene for a {omega}12 desaturase from another species which does normally accumulate petroselinic acid. 19 figs.

  14. Analysis of potential combustion source impacts on acid deposition using an independently derived inventory. Volume II, appendices

    SciTech Connect (OSTI)

    Not Available

    1983-12-01T23:59:59.000Z

    This document contains 2 appendices. The first documents the methodologies used to calculate production, unit energy consumption, fuel type and emission estimates for 16 industries and 35 types of facilities utilizing direct-fired industrial combustion processes, located in 26 states (and the District of Columbia) east of the Mississippi River. As discussed in the text of this report, a U.S. total of 16 industries and 45 types of facilities utilizing direct-fired combustion processes were identified by an elimination type method that was developed based on evaluation of fuel use in industrial SIC codes 20-39 to identify pollutant sources contributing to acid rain. The final population included only plants that have direct-fired fuel consumption greater than or equal to 100 x 10/sup 9/ Btu/yr of equivalent energy consumption. The goal for this analysis was to provide at least a 1980 base year for the data. This was achieved for all of the industries and in fact, 1981 data were used for a number of the industries evaluated. The second contains an analysis of all consumption of major fossil fuels to: (1) identify all fuel usage categories, and (2) identify the kinds of combustion equipment used within each category. This analysis provides a frame of reference for the balance of the study and permits using an energy accounting methodology to quantify the degree to which the inventoried sources in individual consuming sectors are complete and representative of the total population for the sector.

  15. acid sensitive protein: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The adipocyte fatty acid-binding protein aP2 regulates systemic glucose (more) Shum, Bennett Oh Vic 2007-01-01 2 Protein structures uncovered Amino acids are molecules and are...

  16. Protection from hypertension in mice by the Mediterranean diet is mediated by nitro fatty

    E-Print Network [OSTI]

    Hammock, Bruce D.

    Protection from hypertension in mice by the Mediterranean diet is mediated by nitro fatty acid-induced hypertension model in wild-type (WT) but not KI mice. Furthermore, EET/dihydroxy-epoxyeicosatrienoic acid accounting for protection from hypertension afforded by the Mediterranean diet. thiol | cardiovascular

  17. The fatty liver dystrophy (fld) mutation: Developmentally related alterations in hepatic triglyceride metabolism and protein expression

    SciTech Connect (OSTI)

    Reue, K.; Rehnmark, S.; Cohen, R.D.; Leete, T.H.; Doolittle, M.H. [West Los Angeles VA Medical Center, CA (United States). Lipid Research Lab.; [Univ. of California, Los Angeles, CA (United States). Dept. of Medicine; Giometti, C.S.; Mishler, K. [Argonne National Lab., IL (United States); Slavin, B.G. [Univ. of Southern California, Los Angeles, CA (United States)

    1997-07-01T23:59:59.000Z

    Fatty liver dystrophy (fld) is an autosomal recessive mutation in mice characterized by hypertriglyceridemia and development of a fatty liver in the early neonatal period. Also associated with the fld phenotype is a tissue-specific deficiency in the expression of lipoprotein lipase and hepatic lipase, as well as elevations in hepatic apolipoprotein A-IV and apolipoprotein C-II mRNA levels. Although these lipid abnormalities resolve at the age of weaning, adult mutant mice exhibit a peripheral neuropathy associated with abnormal myelin formation. The fatty liver in fld/fld neonates is characterized by the accumulation of large triglyceride droplets within the parenchymal cells, and these droplets persist within isolated hepatocytes maintained in culture for several days. To identify the metabolic defect that leads to lipid accumulation, the authors investigated several aspects of cellular triglyceride metabolism. The mutant mice exhibited normal activity of acid triacylglycerol lipase, an enzyme thought to be responsible for hydrolysis of dietary triglycerides in the liver. Metabolic labeling studies performed with oleic acid revealed that free fatty acids accumulate in the liver of 3 day old fld/fld mice, but not in adults. This accumulation in liver was mirrored by elevated free fatty acid levels in plasma of fld/fld neonates, with levels highest in very young mice and returning to normal by the age of one month. Quantitation of fatty acid oxidation in cells isolated from fld/fld neonates revealed that oxidation rate is reduced 60% in hepatocytes and 40% in fibroblasts; hepatocytes from adult fld/fld mice exhibited an oxidation rate similar to those from wild-type mice.

  18. An efficient didehydroxylation method for the biomass-derived polyols glycerol and erythritol. Mechanistic studies of a formic acid-mediated

    E-Print Network [OSTI]

    of solid acid catalysts6 and in super- critical aqueous media7 to afford the unsaturated aldehyde acrolein-induced dehydration of glycerol to acrolein via a 1,2-hydrogen shift pathway, followed by reduction of the aldehyde

  19. Antibacterial Activities of Liposomal Linolenic Acids against Antibiotic-Resistant Helicobacter pylori

    E-Print Network [OSTI]

    Zhang, Liangfang

    , California 92093, United States ABSTRACT: Helicobacter pylori (H. pylori) infection with its vast prevalence nanotherapeutic to treat antibiotic-resistant H. pylori infection. KEYWORDS: Helicobacter pylori, bacterial infection, free fatty acid, linolenic acid, liposome INTRODUCTION Helicobacter pylori (H. pylori) colonizes

  20. acid increases zn: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    remains mostly unknown and technically challenging to address because of the short half-life of these chemical messengers in the brain. Mice lacking the enzyme fatty acid amide...

  1. Engineering of bacterial methyl ketone synthesis for biofuels

    E-Print Network [OSTI]

    Goh, Ee-Been

    2012-01-01T23:59:59.000Z

    ketone synthesis for biofuels Ee-Been Goh†† 1,3 , Edward E.microbes for use as biofuels, such as fatty acid ethylother fatty acid-derived biofuels, such as fatty acid ethyl

  2. Synthesis and Purification of Various Acyl-ACP Derivatives Kathy Goodson, McNair Scholar, Virginia State University

    E-Print Network [OSTI]

    Omiecinski, Curtis

    the pyruvate dehydrogenase complex, the branched-chain oxo-acid dehydrogenase complex, the -ketoglutarate responsible for the synthesis of lipoic acid, a unique biological cofactor that is involved in cellular-ACP synthetase. II. Background and Significance Lipoic acid is an eight-carbon fatty acid with a unique 1

  3. Factors Affecting Fatty Acid Composition in Forage and Milk

    E-Print Network [OSTI]

    and concentrations of crude protein (CP) and crude fat, which might be useful as tools to predict FA concentrations

  4. Original article Polyunsaturated fatty acids status in blood, heart,

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    and brain of newborn piglets fed either sow milk or a milk replacer diet JM Alessandri, B Goustard, P

  5. MFR PAPER 1033 Research determin es fatty aCid

    E-Print Network [OSTI]

    ~1 Preparation of Esters \\leth) I e\\ler lIere preparuJ u\\lng the mClhod :r, "ere \\cparated an d Idenlilied u\\lng , Zook E J Powell B Hackley J Emerson J Brooker. and G M Knobl

  6. Aging Impairs Myocardial Fatty Acid and Ketone Oxidation and...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    that aging modifies substrate utilization and alters insulin sensitivity in mouse heart when provided multiple substrates. In vivo cardiac function was measured with...

  7. Production Of Hydroxylated Fatty Acids In Genetically Modified Plants

    DOE Patents [OSTI]

    Hall, David R. (Provo, UT); Fox, Joe (Provo, UT)

    2002-05-21T23:59:59.000Z

    An annular wire harness for use in drill pipe comprising two rings interconnected by one or more insulated conductors. The rings are positioned within annular grooves located within the tool joints and the conductors are fixed within grooves along the bore wall of the pipe. The rings may be recessed within annular grooves in order to permit refacing of the tool joint. The rings are provided with means for coupling a power and data signal from an adjacent pipe to the conductors in such a fashion that the signal may be transmitted along the drill pipe and along an entire drill string.

  8. Mutant Fatty Acid Desaturase and Method for Directed Mutagenesis - Energy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOEThe Bonneville PowerCherries 82981-1cnHighandSWPA / SPRA /Ml'.SolarUSAdvancedMuseum Day at BradburyInnovation

  9. Mutant Fatty Acid Desaturase and Method for Directed Mutagenesis - Energy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOEThe Bonneville PowerCherries 82981-1cnHighandSWPA / SPRA /Ml'.SolarUSAdvancedMuseum Day at

  10. Fatty Acid Biosynthesis Caught in the Act | Stanford Synchrotron Radiation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOEThe Bonneville Power AdministrationField8,Dist. Category UC-lFederalFYRANDOMFailure Modesflow phenomena inFat

  11. A First Look at Yeast Fatty Acid Synthase

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of ScienceandMesa del(ANL-IN-03-032) -Less isNFebruaryOctober 2, AlgeriaQ1 Q2you aA Deep Dive intoF

  12. A First Look at Yeast Fatty Acid Synthase

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of ScienceandMesa del(ANL-IN-03-032) -Less isNFebruaryOctober 2, AlgeriaQ1 Q2you aA Deep Dive intoFA

  13. A First Look at Yeast Fatty Acid Synthase

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of ScienceandMesa del(ANL-IN-03-032) -Less isNFebruaryOctober 2, AlgeriaQ1 Q2you aA Deep Dive intoFAA

  14. A First Look at Yeast Fatty Acid Synthase

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of ScienceandMesa del(ANL-IN-03-032) -Less isNFebruaryOctober 2, AlgeriaQ1 Q2you aA Deep Dive intoFAAA

  15. A First Look at Yeast Fatty Acid Synthase

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742EnergyOnItemResearch >InternshipDepartment ofAugustDecember8threbuildA ComprehensiveachA First

  16. acids fish oils: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    acids fish oils First Page Previous Page 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 Next Page Last Page Topic Index 1 Fatty Acid Composition of Fish Oils...

  17. International Journal of Pharmaceutics 307 (2006) 225231 Interactions of oleic acid and model stratum

    E-Print Network [OSTI]

    Rowat, Amy C.

    2006-01-01T23:59:59.000Z

    deuterium nuclear magnetic resonance to monitor such multilamellar SC dispersions containing either, is not clear. Some have speculated that cis-unsaturated fatty acids such as OA `fluidize' SC lipids

  18. Synthesis of labeled oxalic acid derivatives

    DOE Patents [OSTI]

    Martinez, Rodolfo A. (Santa Fe, NM); Unkefer, Clifford J. (Los Alamos, NM); Alvarez, Marc A. (Santa Fe, NM)

    2004-06-22T23:59:59.000Z

    The present invention is directed to labeled compounds, specifically ##STR1## where each C* is selected from the group consisting of a carbon-12, i.e., .sup.12 C, or a carbon-13, i.e., .sup.13 C and at least one C* is .sup.13 C, R.sup.1 is selected from the group of C.sub.1 -C.sub.4 lower alkyl and aryl, and X is selected from the group of --NR.sup.2 R.sup.3 where R.sup.2 and R.sup.3 are each independently selected from the group of C.sub.1 -C.sub.4 lower alkyl, alkoxy and aryl, --SR.sup.4 where R.sup.4 is selected from the group of C.sub.1 -C.sub.4 lower alkyl, alkoxy and aryl, and --OR.sup.5 where R.sup.5 is selected from the group of C.sub.1 -C.sub.4 lower alkyl, alkoxy and aryl with the proviso that when R.sup.1 is methyl then R.sup.5 is other than methyl, when R.sup.1 is ethyl then R.sup.5 is other than ethyl, and when R.sup.1 is benzyl then R.sup.5 is other than benzyl.

  19. Method for high specific bioproductivity of .alpha.,.omega.-alkanedicarboxylic acids

    DOE Patents [OSTI]

    Mobley, David Paul (Schenectady, NY); Shank, Gary Keith (Rocky Hill, CT)

    2000-01-01T23:59:59.000Z

    This invention provides a low-cost method of producing .alpha.,.omega.-alkanedicarboxylic acids. Particular bioconversion conditions result in highly efficient conversion of fatty acid, fatty acid ester, or alkane substrates to diacids. Candida tropicalis AR40 or similar yeast strains are grown in a medium containing a carbon source and a nitrogen source at a temperature of 31.degree. C. to 38.degree. C., while additional carbon source is continuously added, until maximum cell growth is attained. Within 0-3 hours of this point, substrate is added to the culture to initiate conversion. An .alpha.,.omega.-alkanedicarboxylic acid made according to this method is also provided.

  20. Effect of Salt Stress on Purslane and Potential Health Benefits: Oxalic Acid and Fatty Acids Profile

    E-Print Network [OSTI]

    Carvalho, Isabel S.; Teixeira, Mónica; Brodelius, Maria

    2009-01-01T23:59:59.000Z

    IS. 2009. Effects of salt stress on purslane (Portulacaacid concentration occurs when the salt stress concentrationfor higher concentration of salt in both purslanes. In GL

  1. Antimicrobial Property of Lauric Acid Against Propionibacterium Acnes: Its Therapeutic Potential

    E-Print Network [OSTI]

    Zhang, Liangfang

    et al., 1999). Previous studies have found that P. acnes stimulates the production of proinflammatory of lauric acid (C12:0), a middle chain-free fatty acid commonly found in natural products, have been shown treatment as a natural antibiotic against Propionibacterium acnes (P. acnes), which promotes follicular

  2. The Production and Analysis of Biodiesel from Waste Chicken Skin and Pork Skin Fat and a Comparison of Fuel Properties to Petroleum Derived Diesel Fuel

    E-Print Network [OSTI]

    Krish T Bharat; Agni Bhattacharya

    Abstract—People today are increasingly health conscious and therefore shopkeepers tend to dispose of fatty chicken and pork skin. Chicken and pork skins thus are sources of solid waste that are usually not utilized. This paper deals with the production of useful biodiesel from utilizing the waste chicken and pork skins. Fat from the waste chicken and pork skins (sourced from local shops), was first extracted and subjected to transesterification. The products of transesterification were FAME (Fatty acid methyl esters) and glycerol. The FAME produced was tested for five parameters namely calorific value, pour point and cloud point when compared to ASTM E2515-11 standard values. Comparison of the obtained values of the five parameters with the standard values for diesel was performed to determine the viability of the biodiesel produced. The results of this experiment showed that the calorific values of FAME produced from chicken skin and pork skin fat were close to that of petroleum derived diesel. However, two test parameters namely kinematic viscosity and pour point differed when compared to diesel; this problem can be circumvented by modifying an automobile’s internal combustion engine. Due to the relatively high yield value of biodiesel, it is feasible to utilize chicken skin and pork skin fat at a rural level to produce FAME that can be an alternative to diesel in this time of acute fuel scarcity.

  3. Effets d'infusions d'un mlange complet d'acides gras volatils en cours de repas sur la prise alimentaire de vaches taries

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    Effets d'infusions d'un mélange complet d'acides gras volatils en cours de repas sur la prise-Gilles, 35590 L'Hermitage, France Summary ― Infusions of 3 or 6 mol of a volatile fatty acid mixture dépressifs des infusions d'acides gras volatils (AGV) dans le rumen sur l'ingestion d'aliments ont été

  4. Factors determining the progression of nonalcoholic fatty liver disease; the role of abnormal fatty acid and glucocorticoid metabolism 

    E-Print Network [OSTI]

    MacFarlane, David Peter

    2011-11-25T23:59:59.000Z

    Obesity and insulin resistance are associated with a constellation of features including hypertension, dyslipidaemia, type 2 diabetes, and premature cardiovascular disease, collectively termed the metabolic syndrome. ...

  5. Process for the preparation of 3,4-dihydroxybutanoic acid and salts thereof

    DOE Patents [OSTI]

    Hollingsworth, Rawle I. (Haslett, MI)

    1994-01-01T23:59:59.000Z

    A process for the preparation of 3,4-dihydroxybutanoic acid (1) and salts thereof from a glucose source containing 1,4-linked glucose as a substituent is described. The process uses an alkali metal hdyroxide and hydrogen peroxide to convert the glucose source to (1). The compound (1) is useful as a chemical intermediate to naturally occurring fatty acids and is used to prepare 3,4-dihydroxybutanoic acid-gamma-lactone (2) and furanone (3), particularly stereoisomers of these compounds.

  6. Process For The Preparation Of 3,4-Dihyd Roxybutanoic Acid And Salts Thereof

    DOE Patents [OSTI]

    Hollingsworth, Rawle I. (Haslett, MI)

    1994-06-07T23:59:59.000Z

    A process for the preparation of 3,4-dihydroxybutanoic acid (1) and salts thereof from a glucose source containing 1,4-linked glucose as a substituent is described. The process uses an alkali metal hdyroxide and hydrogen peroxide to convert the glucose source to (1). The compound (1) is useful as a chemical intermediate to naturally occurring fatty acids and is used to prepare 3,4-dihydroxybutanoic acid-gamma-lactone (2) and furanone (3), particularly stereoisomers of these compounds.

  7. ascorbic acid oxidase: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Formation of a fluorescent compound. derivative of the vitamin is prevented by forming a boric acid-dehydro- ascorbic acid complex prior to addition of the diamine solution. This...

  8. Three-dimensional Modeling of Acid Transport and Etching in a Fracture 

    E-Print Network [OSTI]

    Oeth, Cassandra V

    2013-11-25T23:59:59.000Z

    -dimensional acid transport and reaction within a fracture to yield the etched width created by acid along the fracture. The conductivity is calculated with the simulator derived acid-etched width, using correlations recently developed that reflect the small scale...

  9. Long range transport of acid rain precursors

    E-Print Network [OSTI]

    Fay, James A.

    1983-01-01T23:59:59.000Z

    A model of the long range transport of primary and secondary pollutants derived by Fay and Rosenzweig (1) is applied to the problem of the transport of acid rain precursors. The model describes the long term average (annual ...

  10. Synthesis and Biological Interest of Structured Docosahexaenoic Acid-Containing Triacylglycerols and Phospholipids

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    (CarMeN), Univ-Lyon 1, Inra, INSA-Lyon, IMBL, Villeurbanne, France Abstract: In light of rapid progress consumption of fats, obesity has espe- cially emerged as one of the major cardiovascular risk factor ac polyunsaturated fatty acids (LC n-3 PUFAs) from fish oils, such as docosahexaenoic (DHA; 22:6n-3

  11. The role of docosahexaenoic acid in mediating mitochondrial membrane lipid peroxidation and apoptosis in colonocytes.

    E-Print Network [OSTI]

    Ng, Yee Voon

    2005-11-01T23:59:59.000Z

    ) or no fatty acid (control) for 72 h with or without 5 mM butyrate for the final 6-24 h. Real time analysis of cellular membrane lipid oxidation, as indicated by oxidation of a lipophilic vital dye, mitochondrial permeability transition (MPT), as characterized...

  12. Derivation of a human equivalent concentration for n-butanol using a physiologically based pharmacokinetic model for n-butyl acetate and metabolites n-butanol and n-butyric acid

    SciTech Connect (OSTI)

    Teeguarden, Justin G.; Deisinger, P. J.; Poet, Torka S.; English, J C.; Faber, W D.; Barton, H. A.; Corley, Rick A.; Clewell, III, H. J.

    2005-05-01T23:59:59.000Z

    The metabolic series (family) approach for risk assessment uses a dosimetry-based analysis to develop toxicity information for a group of metabolically linked compounds using pharmacokinetic (PK) data for each compound and toxicity data for the parent compound. An initial physiologically-based pharmacokinetic (PBPK) model was developed to support the implementation of the metabolic series approach for n-butyl acetate and its subsequent metabolites, n-butanol, and n-butyric acid (the butyl series) (Barton et al. 2000). In conjunction with pilot pharmacokinetic studies, the model was used to design the definitive intravenous (i.v.) PK studies. Rats were implanted with dual indwelling cannulae and administered test compounds by i.v. bolus dose, i.v. infusion, or by inhalation in a recirculating closed chamber. Hepatic, vascular and extravascular metabolic constants for metabolism were estimated by fitting the model to the blood time course data from these experiments. The respiratory bioavailability of n-butyl acetate and n-butanol was estimated from closed chamber inhalation studies and measured ventilation rates. The resulting butyl series PBPK model successfully reproduces the blood time course of these compounds following i.v. administration, and inhalation exposure to n-butyl acetate and n-butanol. A fully scaled human version of the model successfully reproduces arterial blood n-butanol kinetics following inhalation exposure to n-butanol. These validated i.v (rat) and inhalation route models (rat, butyl acetate, n-butanol; human, butanol only) can be used to support species and dose-route extrapolations required for risk assessment of butyl series family of compounds. Further, this work demonstrates the usefulness of i.v. kinetic data for parameterization of systemic metabolism and the value of collaboration between experimentalists and kineticists in the development of PBPK models. The product of this effort, validated rat and human PBPK models for the butyl series compounds, illustrates the effectiveness of broad multi-institutional public/private collaborations in the pursuit of developing state of the art tools for risk assessment.

  13. Effets sur la digestion et le mtabolisme des vaches laitires d'infusions d'acides gras volatils dans le rumen

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    Effets sur la digestion et le métabolisme des vaches laitières d'infusions d'acides gras volatils.N.R.A., Saint-Gilles, 35590, L'Hermitage, France. Summary. Effects of !uminal infusion of volatile fatty acids and duodenal infusion of caseinate on digestion and metabolism in the dairy cow. II. - General and mammary

  14. Effets sur la digestion et le mtabolisme des vaches laitires d'infusions d'acides gras volatils dans le rumen

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    Effets sur la digestion et le métabolisme des vaches laitières d'infusions d'acides gras volatils.N.R.A. Saint-Gilles, 35590 L'Hermitage, France Summary. Effects of ruminal infusion of volatile fatty acids and duodenal infusion of casei- nate on digestion and metabolism in the dairy cow. l. - Production

  15. Identification of arachidonic acid and its metabolism in Gulf of Mexico shrimp

    E-Print Network [OSTI]

    Lilly, Martha Lae

    1980-01-01T23:59:59.000Z

    pattern of double bonds, and it was thus identified as azachidonic acid. A series of experiments was then done to study the synthesis o arachidonic acid (20:4w6) from linoleic acid (18:2w6) in microsomes prepared from shrimp hepatopancreas and tail... allows the *The number preceding the colon indicates the number of carbons in the fatty acid chain. The number following the colon indicates the number of double bonds in the chain. mn signifies that the first double bond is at the nth carbon counting...

  16. Biological Production of a Hydrocarbon Fuel Intermediate Polyhydroxybutyrate (PHB) from a Process Relevant Lignocellulosic Derived Sugar (Poster)

    SciTech Connect (OSTI)

    Wang, W.; Mittal, A.; Mohagheghi, A.; Johnson, D. K.

    2014-04-01T23:59:59.000Z

    PHAs are synthesized by many microorganisms to serve as intracellular carbon storage molecules. In some bacterial strains, PHB can account for up to 80% of cell mass. In addition to its application in the packaging sector, PHB also has great potential as an intermediate in the production of hydrocarbon fuels. PHB can be thermally depolymerized and decarboxylated to propene which can be upgraded to hydrocarbon fuels via commercial oligomerization technologies. Cupriavidus necator is the microorganism that has been most extensively studied and used for PHB production on an industrial scale; However the substrates used for producing PHB are mainly fructose, glucose, sucrose, fatty acids, glycerol, etc., which are expensive. In this study, we demonstrate production of PHB from a process relevant lignocellulosic derived sugar stream, i.e., saccharified slurry from pretreated corn stover. The strain was first investigated in shake flasks for its ability to utilize glucose, xylose and acetate. In addition, the strain was also grown on pretreated lignocellulose hydrolyzate slurry and evaluated in terms of cell growth, sugar utilization, PHB accumulation, etc. The mechanism of inhibition in the toxic hydrolysate generated by the pretreatment and saccharification process of biomass, was also studied.

  17. Carbonic Acid Pretreatment of Biomass

    SciTech Connect (OSTI)

    G. Peter van Walsum; Kemantha Jayawardhana; Damon Yourchisin; Robert McWilliams; Vanessa Castleberry

    2003-05-31T23:59:59.000Z

    This project sought to address six objectives, outlined below. The objectives were met through the completion of ten tasks. 1) Solidify the theoretical understanding of the binary CO2/H2O system at reaction temperatures and pressures. The thermodynamics of pH prediction have been improved to include a more rigorous treatment of non-ideal gas phases. However it was found that experimental attempts to confirm theoretical pH predictions were still off by a factor of about 1.8 pH units. Arrhenius experiments were carried out and the activation energy for carbonic acid appears to be substantially similar to sulfuric acid. Titration experiments have not yet confirmed or quantified the buffering or acid suppression effects of carbonic acid on biomass. 2) Modify the carbonic acid pretreatment severity function to include the effect of endogenous acid formation and carbonate buffering, if necessary. It was found that the existing severity functions serve adequately to account for endogenous acid production and carbonate effects. 3) Quantify the production of soluble carbohydrates at different reaction conditions and severity. Results show that carbonic acid has little effect on increasing soluble carbohydrate concentrations for pretreated aspen wood, compared to pretreatment with water alone. This appears to be connected to the release of endogenous acids by the substrate. A less acidic substrate such as corn stover would derive benefit from the use of carbonic acid. 4) Quantify the production of microbial inhibitors at selected reaction conditions and severity. It was found that the release of inhibitors was correlated to reaction severity and that carbonic acid did not appear to increase or decrease inhibition compared to pretreatment with water alone. 5) Assess the reactivity to enzymatic hydrolysis of material pretreated at selected reaction conditions and severity. Enzymatic hydrolysis rates increased with severity, but no advantage was detected for the use of carbonic acid compared to water alone. 6) Determine optimal conditions for carbonic acid pretreatment of aspen wood. Optimal severities appeared to be in the mid range tested. ASPEN-Plus modeling and economic analysis of the process indicate that the process could be cost competitive with sulfuric acid if the concentration of solids in the pretreatment is maintained very high (~50%). Lower solids concentrations result in larger reactors that become expensive to construct for high pressure applications.

  18. Modular pathway engineering of microbial fatty acid metabolism for the synthesis of branched acids, alcohols, and alkanes

    E-Print Network [OSTI]

    Sheppard, Micah J. (Micah James)

    2014-01-01T23:59:59.000Z

    Historically, microbial platforms have been used to synthesize a variety of chemical products and potential biofuels. More recently, increasingly complex metabolic pathways have been engineered by using novel hosts, modifying ...

  19. Essential versus potentially toxic dietary substances: A seasonal comparison of essential fatty acids and methyl mercury

    E-Print Network [OSTI]

    Mazumder, Asit

    , Asit Mazumder a a Water and Aquatic Sciences Research Program, University of Victoria, Department of Biology, P.O. Box 3020, Stn. CSC, Victoria, BC V8W 3N5, Canada b Aquatic Ecosystem Management Research, Burlington, ON L7R 4A6, Canada Received 21 August 2007; received in revised form 15 November 2007; accepted

  20. The role of Ncb5or in fatty acid metabolism and redox homeostasis

    E-Print Network [OSTI]

    Xu, Ming

    2011-12-31T23:59:59.000Z

    The endoplasmic reticulum (ER)-associated NADH cytochrome b5 oxidoreductase (Ncb5or) is widely distributed in animal tissues. It contains two redox domains that are homologous to microsomal cytochrome b5 (Cyb5A) at the N ...

  1. Influence of Fatty Alcohols and Acids on the Clarity and Biota of Impounded Reservoirs 

    E-Print Network [OSTI]

    Davis, W. B.; Reynolds, T. D.

    1969-01-01T23:59:59.000Z

    This project presents results on a laboratory and field investigation of some of the biological and physical effects as a result of the use of a 1:1 mixture of hexadecanol and octadecanol. Laboratory investigations were ...

  2. Hydrogen-isotopic variability in fatty acids from Yellowstone National Park hot spring microbial communities

    E-Print Network [OSTI]

    of Geological and Planetary Sciences, California Institute of Technology, Pasadena, CA 91125, USA b Division intermediate fractionations similar in magnitude to those expressed by higher plants. These data support of seawater is nearly invariant, these data sug- gest the existence of controlling factors independent

  3. Purification and expression of fatty acid binding proteins in chicken liver and intestine

    E-Print Network [OSTI]

    Sewell, Julia Ellen

    1988-01-01T23:59:59.000Z

    -mercaptoethanol, 0. 54 Sarkosyl, O. lt Antifoam A) until mixture was smooth. 18 Cesium chloride (8 g) was added and blended for a few seconds. Homogenate was then layered onto a 7 ml cushion of CsC1 solution (5. 7 M CsCl, 0. 1 M EDTA, pH 7. 5... was removed and added to 990 ul of ddH20 in a cuvette. A blank of ddH20 was used and optical densities were read at wavelengths 260 nm and 280 nm. The ratio of wavelengths 260/280 was determined to assess protein contamination and degradation of RNA...

  4. Investigating fatty acid biosynthesis within the algal chloroplast using Chlamydomonas reinhardtii as a model

    E-Print Network [OSTI]

    Blatti, Jillian L.

    2012-01-01T23:59:59.000Z

    Energy (La Jolla, CA), Solazyme (San Francisco, CA), Aurorawith the NREL and Solazyme, Exxon Mobil with SyntheticDOE has awarded funds to Solazyme and Sapphire Energy to

  5. Glucose oxidation in heart-type fatty acid binding protein null mice

    E-Print Network [OSTI]

    Adhikari, Sean

    2006-10-30T23:59:59.000Z

    . In the complex protocol, the mutation first inhibited the synthesis and accumulation of triglycerides and then their mobilization; with this protocol, the mutation increased glucose oxidation in both CHOW- and HFD-solei. We conclude: (i) H-FABP mediates a non...

  6. Digestion and rumen volatile fatty acid production by heifers fed reconstituted sorghum grain

    E-Print Network [OSTI]

    Pantin, Eduardo Jose

    1969-01-01T23:59:59.000Z

    & high in ccncent' ate. VI. The effect o? the physical snd biochmrtcal process of the reticulo-runan. J. Dairy Res. 22!270. l3aich, C. C. , J. T. Reid and J, W. Stroud. 1957. Factors influ- e!ruing t3:e rote of excretion of aiministercd chromium ses...

  7. Impact of Omega-3 Polyunsaturated Fatty Acids on Bone Adaptations to Simulated Resistance Training

    E-Print Network [OSTI]

    Camp, Kaleigh Ann

    2013-04-30T23:59:59.000Z

    properties of proximal tibia were measured using in vivo peripheral quantitative CT. Bone formation rate was quantified on the periosteal the surface by standard bone histomorphometry after intraperitoneal injections of calcein. There was a significant main...

  8. Investigating fatty acid biosynthesis within the algal chloroplast using Chlamydomonas reinhardtii as a model

    E-Print Network [OSTI]

    Blatti, Jillian L.

    2012-01-01T23:59:59.000Z

    for bioenergy (i.e. bio-H 2 , bioethanol, and biodiesel),and carbohydrates fermented to bioethanol or converted toincluding biohydrogen, bioethanol, biodiesel, and

  9. Fatty acid composition and distribution in the blubber of bottlenose dolphins (Tursiops truncatus)

    E-Print Network [OSTI]

    Samuel, Asha Melina

    2000-01-01T23:59:59.000Z

    bottlenose dolphin (Tursiops truncatus) sampled at the trailing edge of the dorsal fin, dorsal surface. Visible are three blubber layers, along with the skin and underlying muscle tissue. . . . . . . . . . . . . . . . . . . . . . Blubber sampling... and Atmospheric Administration. . 17 Classification and regression tree analysis for all samples (n=61) from 22 animals at the trailing edge of the dorsal fin, dorsal surface, separated by blubber layer 24 Classification and regression tree analysis for sub...

  10. Investigating fatty acid biosynthesis within the algal chloroplast using Chlamydomonas reinhardtii as a model

    E-Print Network [OSTI]

    Blatti, Jillian L.

    2012-01-01T23:59:59.000Z

    microalgae for sustainable biodiesel. (2012) Blatti, J.L. ,energy within pond scum: Biodiesel from algal lipids. J.energy within pond scum: biodiesel from algal lipids. ”

  11. Polyploid genome of Camelina sativa revealed by isolation of fatty acid synthesis genes

    E-Print Network [OSTI]

    2010-01-01T23:59:59.000Z

    oil as a feedstock for biodiesel production. Industrialthe properties of C. sativa biodiesel are already well6], and both seed oil and biodiesel from C. sativa were used

  12. Effects of dietary polyunsaturated fatty acid supplementation on urinary eicosanoids in canine chronic renal failure

    E-Print Network [OSTI]

    Crocker, Raquel Cristina

    1996-01-01T23:59:59.000Z

    , with PGE2 being first extracted on C 1 8silica columns. Values were normalized by urinary creatinine (UrCr) concentrations. A PGE2:TXB2 ratio was calculated as an index of renal vasodilation. Glomerular filtration rate (GFR) was determined at each sample...

  13. Modulation of purified murine T cell subset activation by dietary n-3 Polyunsaturated fatty acids

    E-Print Network [OSTI]

    Arrington, Jennifer L

    2000-01-01T23:59:59.000Z

    of glycerophospholipid. These lipids are localized to predominantly either the outer or inner leaflet of the plasma membrane (Zachowski et al. 1993) and, thus, membrane lipid composition is vital since components of the plasma membrane can generate second messengers... association of TcR to RAFTs is necessary for enhanced TcR signal generation (Yashiro-Ohtari et al. 2000, Xavier et al. 1998) Additionally, tyrosine phosphorylation of ITAMs occurs in RAFTs (Ilangumaran et al. 2000). Thus the membrane lipid composition could...

  14. Investigating fatty acid biosynthesis within the algal chloroplast using Chlamydomonas reinhardtii as a model

    E-Print Network [OSTI]

    Blatti, Jillian L.

    2012-01-01T23:59:59.000Z

    water. The ligand was prepared by extracting the stearate-phosphopantetheine structure from the structure of spinach

  15. Investigating fatty acid biosynthesis within the algal chloroplast using Chlamydomonas reinhardtii as a model

    E-Print Network [OSTI]

    Blatti, Jillian L.

    2012-01-01T23:59:59.000Z

    Physical Solutions for Hydrogen Storage. Angewandte Chemie -Practical solutions of hydrogen storage. Angewandte Chemie -economy’ are aware that hydrogen storage and transport are

  16. SHORT COMMUNICATION Unsaturated fatty acids inhibit MP2C, a protein

    E-Print Network [OSTI]

    Hirt, Heribert

    or to wound-related compounds (BoÈ gre etal., 1997; Seo et al., 1995; Stratmann and Ryan, 1997). Moreover (Seo et al., 1995; Seo et al., 1999). Whereas MAPK activation relies on phosphorylation

  17. Investigating fatty acid biosynthesis within the algal chloroplast using Chlamydomonas reinhardtii as a model

    E-Print Network [OSTI]

    Blatti, Jillian L.

    2012-01-01T23:59:59.000Z

    for biofuel production: Perspectives and advances. Plantfor biofuel production: perspectives and advances. Plantfor biofuel production: perspectives and advances. Plant

  18. Constructing and engineering fatty acid metabolic pathways for the production of fuels and chemicals

    E-Print Network [OSTI]

    Steen, Eric James

    2010-01-01T23:59:59.000Z

    Russell, D. W. Mammalian wax biosynthesis. I. IdentificationACB1 5.3.5. Expressing the wax ester synthase, atfA 5.3.6.A. AAC45217 calcoaceticus atfA, wax ester synthase A. baylyi

  19. Associations of Fatty Acids in Cerebrospinal Fluid with Peripheral Glucose Concentrations and Energy Metabolism

    E-Print Network [OSTI]

    Jumpertz, Reiner; Guijarro, Ana; Pratley, Richard E.; Mason, Clinton C.; Piomelli, Daniele; Krakoff, Jonathan

    2012-01-01T23:59:59.000Z

    composition was measured by dual energy X-ray absorptiometrycomposition was measured by dual-energy X-ray absorptio-

  20. An investigation of the origin and metabolism of the polyunsaturated fatty acids found in aquatic organisms

    E-Print Network [OSTI]

    Kelly, Peter B.

    1958-01-01T23:59:59.000Z

    the dietary experiment iising rock- FIGURE 13 bass. Starch base diets. A - The oil of rockbass on their natural diet. B - The oil of rockbass on the fat-free diet. C - The oil of rockbass on the fresh-water diet D - The oil of rockbass on the marine diet... dietary changes in a manner similar to that of fish. In the crab experiment it can be seen that the crabs on the natural diet have fat generally reminiscent of the fats of marine fish, which under normal conditions would be their major fat source...

  1. Constructing and engineering fatty acid metabolic pathways for the production of fuels and chemicals

    E-Print Network [OSTI]

    Steen, Eric James

    2010-01-01T23:59:59.000Z

    production. The products were detected in both the pellet andproduction, 1 mL of culture was centrifuged, 30 s, 20,000 x g and the supernatant was separated from the pellet.

  2. ORIGINAL ARTICLE Fatty Acid Profile of Sunshine Bass: I. Profile Change is Affected

    E-Print Network [OSTI]

    . Currently, 45% of the fish consumed worldwide are cultured [1]. Assuming per capita seafood consumption­8]. As a result, both nutritional and medical communities have rec- ommended increasing human consumption of LC

  3. Modifying yolk fatty acid composition to improve the health quality of shell eggs 

    E-Print Network [OSTI]

    Van Elswyk, Mary Elizabeth

    1990-01-01T23:59:59.000Z

    . Reesterification of free cholesterol is catalyzed by the ACAT enzyme. When free cholesterol is abundant inside the cell, new LDL receptors are not synthesized, and so the uptake of additional cholesterol from plasma LDL is blocked. De Schrijver and Privett... to apoprotein B and E receptors causing competition with LDL for removal mechanisms, or reduced receptor- mediated removal of LDL. Packard et al. (1984) suggested that in response to fish oil consumption the liver secretes both triglyceride rich, large VLDL...

  4. Investigating fatty acid biosynthesis within the algal chloroplast using Chlamydomonas reinhardtii as a model

    E-Print Network [OSTI]

    Blatti, Jillian L.

    2012-01-01T23:59:59.000Z

    transport and storage. Along with the aforementioned goals to exploit native metabolic pathways to produce biofuels

  5. Investigating fatty acid biosynthesis within the algal chloroplast using Chlamydomonas reinhardtii as a model

    E-Print Network [OSTI]

    Blatti, Jillian L.

    2012-01-01T23:59:59.000Z

    of biodiesel fuel from microalgae. Enzymatic Conversion ofHydrogen fuel production by transgenic microalgae. In: LeonMicroalgae are a promising feedstock for renewable fuels,

  6. Author's personal copy Culture performance and tissue fatty acid compositions of yellow perch

    E-Print Network [OSTI]

    . Introduction Historically, the aquaculture industry has relied on fish meal and fish oil as the main sources and more avail- able than fish oil, although market price variation and availability play a role been about the same price as fish oil, but recently has been considerably less expensive. Flaxseed oil

  7. Volatile fatty acid fermentation of AFEX-treated newspaper and bagasse by rumen microorganisms

    E-Print Network [OSTI]

    Blasig, Jorge Dari?o

    1991-01-01T23:59:59.000Z

    days and filters were replaced every 12 hours. Loading rate does not include alfalfa. H A D Figure 4. Continuous culture system. A, buffer reservoir, B, fermenter; C, overflow effluent reservoir; D, filtered effluent reservoir; E, gas collector; F..., G, peristaltic pumps; H, filter, I, magnetic stir plate, containing up to 4 fermenter vessels; J, temperature bath circulator 28 FIGURE 5. Fermentor diagram. 1, fermenter lid; 2, buffer input; 3, gas outlet; 4, overflow port; 5, filter; 6...

  8. Effect of fermentable carbohydrates on volatile fatty acids, ammonia and mineral absorption in the rat caecum

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    intestine on caecal digestion and absorption have been studied in the rat. Even in diets lacking these carbo of the urea nitrogen was used in bacterial protein synthesis. Arteriovenous differences in the caecum showed, for which hydrolysing enzymes are lacking or poorly efficient in the small i ntesti ne. These carbohydrates

  9. Investigating fatty acid biosynthesis within the algal chloroplast using Chlamydomonas reinhardtii as a model

    E-Print Network [OSTI]

    Blatti, Jillian L.

    2012-01-01T23:59:59.000Z

    renewable and sustainable biofuels using a photosyntheticFebruary 20, 2011 Algae Biofuels Symposium Invited Lecture.to land-based crops for biofuels. Current Opinion in

  10. Constructing and engineering fatty acid metabolic pathways for the production of fuels and chemicals

    E-Print Network [OSTI]

    Steen, Eric James

    2010-01-01T23:59:59.000Z

    of biodiesel and ethanol biofuels. Proc Natl Acad Sci U Sanalysis of alternative biofuels produced from geneticallychain higher alcohols as biofuels. Nature 451, 86-9 (2008).

  11. Relationships of the minerals and fatty acid contents in processed turkey meat products

    E-Print Network [OSTI]

    Ferreira, Márcia M. C.

    -970, Campinas, SP, Brazil b Instituto de Tecnologia de Alimentos, ITAL- CP 139, 13073-001 Campinas SP, Brazil

  12. Investigating fatty acid biosynthesis within the algal chloroplast using Chlamydomonas reinhardtii as a model

    E-Print Network [OSTI]

    Blatti, Jillian L.

    2012-01-01T23:59:59.000Z

    of interest for biofuels Microalgae store solar energy asS (2011) Microalgae for the production of biofuels and bio-produce liquid biofuels via the sun using microalgae as a

  13. Imflammatory response in horses fed diets containing omega-6 and omega-3 fatty acids

    E-Print Network [OSTI]

    Wilson, Kristopher Ray

    2003-01-01T23:59:59.000Z

    ~m~y~i A less commonly used, but more potent, acute phase protein inflammatory marker is Serum Amyloid A (SAA) (Hulten et al. , 1999). Serum Amyloid A is an acute phase protein that is antigenically the same as amyloid A, which is the major protein... mg/1, but noted a wide range in peak values, from 46-286 mg/1. for horses that were induced with arthritis. Pepys et al. (1989) also reported variations in peak concentrations of SAA in horses following cold elective surgery. Both instances suggest...

  14. Investigating fatty acid biosynthesis within the algal chloroplast using Chlamydomonas reinhardtii as a model

    E-Print Network [OSTI]

    Blatti, Jillian L.

    2012-01-01T23:59:59.000Z

    biodiesel at two times the cost of petroleum with current technology [Biodiesel production by direct methanolysis of oleaginous microbial biomass. Journal of Chemical Technologytechnology, we describe the extraction of algal lipids and explore their transesterification into biodiesel.

  15. Volatile fatty acid fermentation of lime-treated biomass by rumen microorganisms

    E-Print Network [OSTI]

    Rapier, Charles Robert

    1995-01-01T23:59:59.000Z

    concentration was studied. A counter-current fermentation system was also simulated to determine if it provided advantages over a conventional batch fermentor. All of the substrates studied produced similar amounts of VFA's. Approximately 7- I 0 g/L of acetic... sugars. This is accomplished by hydrolytic enzymes. However, cellulose possesses a recalcitrant crystalline structure and is insoluble in aqueous solutions, making it highly resistant to enzymatic degradation. Compounding this problem is lignin, which...

  16. Concentrating aqueous volatile fatty acid salt solutions using a tertiary amine mixture

    E-Print Network [OSTI]

    Gaskin, David J

    1997-01-01T23:59:59.000Z

    Lee (1993) has shown that tertiary amines are able to hics. extract water from low-concentration calcium acetate and sodium acetate solutions. This thesis extends the previous work to include calcium propionate and butyrate. Amine extraction may...

  17. Investigating fatty acid biosynthesis within the algal chloroplast using Chlamydomonas reinhardtii as a model

    E-Print Network [OSTI]

    Blatti, Jillian L.

    2012-01-01T23:59:59.000Z

    for renewable energy has resurged, and the potential ofrenewable transportation fuel such as biodiesel has the potential to dramatically impact the future energy

  18. Effect of temperature on the fatty acid profile of pecan oil

    E-Print Network [OSTI]

    Bahri, Sami

    1992-01-01T23:59:59.000Z

    University Brison Orchard and analyzed for total oi l content by the Nuclear Magnetic Resonance ( N M R ) method and by the Soxhlet extraction method. Nut samples were separated by diameter and size, and dried overnight at 105C. The N M R instrument used...

  19. Investigating fatty acid biosynthesis within the algal chloroplast using Chlamydomonas reinhardtii as a model

    E-Print Network [OSTI]

    Blatti, Jillian L.

    2012-01-01T23:59:59.000Z

    harness new forms of energy, microalgae have emerged as aFigure 1-1 ). Microalgae naturally accumulate energy-densefor biofuels Microalgae store solar energy as lipids or

  20. Constructing and engineering fatty acid metabolic pathways for the production of fuels and chemicals

    E-Print Network [OSTI]

    Steen, Eric James

    2010-01-01T23:59:59.000Z

    synthesized (Epoch biolabs). pdc and adhB were cloned fromA. baylyi ADP1 AAO17391 pdc, pyruvate decarboxylase Z.of an ethanol pathway (pdc and adhB) and wax esters were

  1. Investigating fatty acid biosynthesis within the algal chloroplast using Chlamydomonas reinhardtii as a model

    E-Print Network [OSTI]

    Blatti, Jillian L.

    2012-01-01T23:59:59.000Z

    biomass feedstocks and yields of biomass feedstocks a) Amount of land requiredOil yields of leading biomass feedstocks. (Adapted from the

  2. The fatty acid esterification pathway in bovine liver and adipose tissue

    E-Print Network [OSTI]

    Wilson, John

    1988-01-01T23:59:59.000Z

    . . . . . . . . , . . . . . . . . . . . . , . . . . . 24 25 13 USED TO DETERMINE DIFFERENCES BETWEEN BREEDS, TISSUES AND EN~S AND TEST FOR INTERACIIONS. ?. ?. .. . . . . . . ?. . . . . . . . . . 26 LIST OF MODELS (continued) Model Page 14. USED TO DETERMINE DIFFERENCES BETWEEN BREEDS, TISSUES.... This inactivation of the micmsomal glycerolphosphate acyltransferase was also observed by others (Declercq et a1. , 1982; Jamdar and Osborne, 1982; Nimmo and Nimmo, 1984) when ?thylmaleimide was used. Nimmo and Nimmo (1984) showed that when 25 irM palmitoyl...

  3. Fatty Acid-Producing Microbes for Generating Medium- and Long-Chain

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOEThe Bonneville Power AdministrationField8,Dist. Category UC-lFederalFYRANDOMFailure Modesflow phenomena

  4. Determination of Total Lipids as Fatty Acid Methyl Esters (FAME) by in situ

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOEThe Bonneville Power AdministrationField Campaign: Potential Application to ARMTransesterification: Laboratory

  5. acidic lipase derived: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and as concluding remarks, the advantages, concerns, and future prospects of enzymatic biodiesel are presented. 1. Lipid Feedstocks The main feedstocks which present paramount...

  6. acid ester derivatives: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    was strarted by preparing sodium ethoxyde from sodium hydroxyde and ethanol and then mixed it with the coconut oil: ratio ethanoloil (1:2), stirring speed 500...

  7. ascorbic acid derivatives: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    to enamine Paris-Sud XI, Universit de 104 Expression of ASCORBATE PEROXIDASE 8 in roots of rice (Oryza sativa L.) seedlings in response to NaCl CiteSeer Summary: Reactive...

  8. ascorbic acid derivative: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    to enamine Paris-Sud XI, Universit de 104 Expression of ASCORBATE PEROXIDASE 8 in roots of rice (Oryza sativa L.) seedlings in response to NaCl CiteSeer Summary: Reactive...

  9. Effets de drivs d'acides gras sur les ingestions volontaires d'aliments chez le mouton

    E-Print Network [OSTI]

    Boyer, Edmond

    Notre-Dame de la Paix, B-5000 Namur, Belgique. Summary. The continuous infusion of different fatty acid the same decreasing effect on the voluntary dry matter intake of adult sheep than the infusion of oleic jours au cours desquelles les sous-groupes reçoivent, en alternance, des infusions continues (1 1/animal

  10. Derivative actions in China 

    E-Print Network [OSTI]

    Lin, Shaowei

    2014-07-02T23:59:59.000Z

    The enactment of derivative action was expected to be actively used by shareholders to protect their interests. In fact, it turned out that this reform effort seemed futile as the right to engage in such actions was ...

  11. Dynamic Derivative Strategies

    E-Print Network [OSTI]

    Liu, Jun

    2003-09-25T23:59:59.000Z

    This paper studies the optimal investment strategy of an investor who can access not only the bond and the stock markets, but also the derivatives market. We consider the investment situation where, in addition to the usual ...

  12. Credit derivatives in Brazil

    E-Print Network [OSTI]

    Rüther, Henrique

    2007-01-01T23:59:59.000Z

    The amounts outstanding of credit derivatives have grown exponentially over the past years, and these financial intruments that allow market participants to trade credit risk have become very popular in Europe and in the ...

  13. Process for the preparation of 3,4-dihydroxybutanoic acid and salts thereof

    DOE Patents [OSTI]

    Hollingsworth, Rawle I. (Haslett, MI)

    1994-01-01T23:59:59.000Z

    A process for the preparation of 3,4-dihydroxybutanoic acid (1) and salts thereof from a glucose source containing 1,4-1inked glucose as a substituent is described. The process uses an alkali metal hdyroxide and hydrogen peroxide to convert the glucose source to (1). The compound (1) is useful as a chemical intermediate to naturally occurring fatty acids and is used to prepare 3,4-dihydroxybutanoic acid-gamma-lactone (2) and furanone (3), particularly stereoisomers of these compounds.

  14. Carrier-mediated transport of valproic acid in BeWo cells, a human trophoblast cell line

    E-Print Network [OSTI]

    Utoguchi, Naoki; Audus, Kenneth L.

    2000-01-01T23:59:59.000Z

    et al., 1998), asymmetric transferrin transport (Van der Ende et al., 1990; Cerneus et al., 1993), asymmetric fatty acid transport (Liu et al., 1997), choline uptake (Eaton and Sooranna, 1998a), glucose modulation of arginine transport (Eaton... Costar Corporation for support of the Cellular and Molecular Biopharmaceutics Handling Laboratory. Utoguchi, N. and Audus, K.L. (2000) Carrier-mediated transport of valproic acid in BeWo cells, a human trophoblast cell line. Int. J. Pharm. 195, 115...

  15. acrylate-acrylic acid dry: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Formation of a fluorescent compound. derivative of the vitamin is prevented by forming a boric acid-dehydro- ascorbic acid complex prior to addition of the diamine solution. This...

  16. Derived Azumaya algebras and generators for twisted derived categories

    E-Print Network [OSTI]

    Toen, Bertrand

    implies the existence of a global compact generator. We present explicit examples of derived Azumaya

  17. Non-essential amino acid metabolism in rats

    E-Print Network [OSTI]

    Crooks, James Darrell

    1971-01-01T23:59:59.000Z

    -ketoglutarate by glutamic dehydrogenase is a major route of synthesis of glutamic acid (15, 33) . This path- way provides for the entrance of ammonia nitrogen into the amino acid pool (34) ~ The nitrogen can be distributed to the amino acids by the transamination... of ammonia in the mammal have been discussed. Due to this close relationship, glutamine can be derived from any compound forming glutamic acid (31, 35) . This is demonstrated by the synthesis of its carbon skeleton from aspartic acid (34), asparagine (29...

  18. Pushing schedule derivation method

    SciTech Connect (OSTI)

    Henriquez, B. [Compania Siderurgica Huachipato S.A., Talcahuano (Chile)

    1996-12-31T23:59:59.000Z

    The development of a Pushing Schedule Derivation Method has allowed the company to sustain the maximum production rate at CSH`s Coke Oven Battery, in spite of having single set oven machinery with a high failure index as well as a heat top tendency. The stated method provides for scheduled downtime of up to two hours for machinery maintenance purposes, periods of empty ovens for decarbonization and production loss recovery capability, while observing lower limits and uniformity of coking time.

  19. Production of Succinic Acid for Lignocellulosic Hydrolysates

    SciTech Connect (OSTI)

    Davison, B.H.; Nghiem, J.

    2002-06-01T23:59:59.000Z

    The purpose of this Cooperative Research and Development Agreement (CRADA) is to add and test new metabolic activities to existing microbial catalysts for the production of succinic acid from renewables. In particular, they seek to add to the existing organism the ability to utilize xylose efficiently and simultaneously with glucose in mixtures of sugars or to add succinic acid production to another strain and to test the value of this new capability for production of succinic acid from industrial lignocellulosic hydrolyasates. The Contractors and Participant are hereinafter jointly referred to as the 'Parties'. Research to date in succinic acid fermentation, separation and genetic engineering has resulted in a potentially economical process based on the use of an Escherichia coli strain AFP111 with suitable characteristics for the production of succinic acid from glucose. Economic analysis has shown that higher value commodity chemicals can be economically produced from succinic acid based on repliminary laboratory findings and predicted catalytic parameters. The initial target markets include succinic acid itself, succinate salts, esters and other derivatives for use as deicers, solvents and acidulants. The other commodity products from the succinic acid platform include 1,4-butanediol, {gamma}-butyrolactone, 2-pyrrolidinone and N-methyl pyrrolidinone. Current economic analyses indicate that this platform is competitive with existing petrochemical routes, especially for the succinic acid and derivatives. The report presents the planned CRADA objectives followed by the results. The results section has a combined biocatalysis and fermentation section and a commercialization section. This is a nonproprietary report; additional proprietary information may be made available subject to acceptance of the appropriate proprietary information agreements.

  20. DOI: 10.1002/cssc.201000111 A Direct, Biomass-Based Synthesis of Benzoic Acid: Formic Acid-Mediated

    E-Print Network [OSTI]

    DOI: 10.1002/cssc.201000111 A Direct, Biomass-Based Synthesis of Benzoic Acid: Formic Acid] Production of industrial aro- matic compounds from biomass resources could provide a sus- tainable derived from benzene in the USA and Western Europe. Phenol is used mainly in the production of phenolic

  1. Antioxidant and Antiradical SiO2 Nanoparticles Covalently Functionalized with Gallic Acid

    E-Print Network [OSTI]

    Daraio, Chiara

    Antioxidant and Antiradical SiO2 Nanoparticles Covalently Functionalized with Gallic Acid Yiannis with no impairment of their RSC. KEYWORDS: nanoantioxidant, DPPH radical, gallic acid, grafting, nanoparticle Zurich, Switzerland *S Supporting Information ABSTRACT: Gallic acid (GA) and its derivatives are natural

  2. Effects of a High Oleic Acid Beef Diet on Cardiovascular Disease Risk Factors of Human Subjects 

    E-Print Network [OSTI]

    Adams, Thaddeus Hunter

    2012-10-19T23:59:59.000Z

    acid were negatively correlated with LDL particle diameter (all P <= 0.05). Because plasma palmitoleic acid was derived from [delta]9 desaturation of palmitic acid in the liver, we conclude that alterations in hepatic stearoyl-CoA desaturase activity...

  3. Cleavage of nucleic acids

    DOE Patents [OSTI]

    Prudent, James R. (Madison, WI); Hall, Jeff G. (Madison, WI); Lyamichev, Victor L. (Madison, WI); Brow, Mary Ann D. (Madison, WI); Dahlberg, James E. (Madison, WI)

    2007-12-11T23:59:59.000Z

    The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

  4. Nucleic acid detection compositions

    DOE Patents [OSTI]

    Prudent, James R. (Madison, WI); Hall, Jeff G. (Madison, WI); Lyamichev, Victor I. (Madison, WI); Brow, Mary Ann (Madison, WI); Dahlberg, James L. (Madison, WI)

    2008-08-05T23:59:59.000Z

    The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

  5. Cleavage of nucleic acids

    DOE Patents [OSTI]

    Prudent, James R. (Madison, WI); Hall, Jeff G. (Waunakee, WI); Lyamichev, Victor I. (Madison, WI); Brow; Mary Ann D. (Madison, WI); Dahlberg, James E. (Madison, WI)

    2010-11-09T23:59:59.000Z

    The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

  6. Nucleic acid detection assays

    DOE Patents [OSTI]

    Prudent, James R.; Hall, Jeff G.; Lyamichev, Victor I.; Brow, Mary Ann; Dahlberg, James E.

    2005-04-05T23:59:59.000Z

    The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

  7. Process for the preparation of lactic acid and glyceric acid

    DOE Patents [OSTI]

    Jackson, James E [Haslett, MI; Miller, Dennis J [Okemos, MI; Marincean, Simona [Dewitt, MI

    2008-12-02T23:59:59.000Z

    Hexose and pentose monosaccharides are degraded to lactic acid and glyceric acid in an aqueous solution in the presence of an excess of a strongly anionic exchange resin, such as AMBERLITE IRN78 and AMBERLITE IRA400. The glyceric acid and lactic acid can be separated from the aqueous solution. Lactic acid and glyceric acid are staple articles of commerce.

  8. Nucleic acid detection kits

    DOE Patents [OSTI]

    Hall, Jeff G.; Lyamichev, Victor I.; Mast, Andrea L.; Brow, Mary Ann; Kwiatkowski, Robert W.; Vavra, Stephanie H.

    2005-03-29T23:59:59.000Z

    The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of nucleic acid from various viruses in a sample.

  9. Microorganisms for producing organic acids

    DOE Patents [OSTI]

    Pfleger, Brian Frederick; Begemann, Matthew Brett

    2014-09-30T23:59:59.000Z

    Organic acid-producing microorganisms and methods of using same. The organic acid-producing microorganisms comprise modifications that reduce or ablate AcsA activity or AcsA homolog activity. The modifications increase tolerance of the microorganisms to such organic acids as 3-hydroxypropionic acid, acrylic acid, propionic acid, lactic acid, and others. Further modifications to the microorganisms increase production of such organic acids as 3-hydroxypropionic acid, lactate, and others. Methods of producing such organic acids as 3-hydroxypropionic acid, lactate, and others with the modified microorganisms are provided. Methods of using acsA or homologs thereof as counter-selectable markers are also provided.

  10. Process Design and Economics for the Conversion of Lignocellulosic Biomass to Hydrocarbons: Dilute-Acid and Enzymatic Deconstruction of Biomass to Sugars and Biological Conversion of Sugars to Hydrocarbons

    SciTech Connect (OSTI)

    Davis, R.; Tao, L.; Tan, E. C. D.; Biddy, M. J.; Beckham, G. T.; Scarlata, C.; Jacobson, J.; Cafferty, K.; Ross, J.; Lukas, J.; Knorr, D.; Schoen, P.

    2013-10-01T23:59:59.000Z

    This report describes one potential conversion process to hydrocarbon products by way of biological conversion of lingnocellulosic-dervied sugars. The process design converts biomass to a hydrocarbon intermediate, a free fatty acid, using dilute-acid pretreatement, enzymatic saccharification, and bioconversion. Ancillary areas--feed handling, hydrolysate conditioning, product recovery and upgrading (hydrotreating) to a final blendstock material, wastewater treatment, lignin combusion, and utilities--are also included in the design.

  11. EMSL - Nuclei acid structure

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    conditions showed a temporary pH decrease, with a concomitant increase in formic acid during exponential growth. Fermentation experiments performed outside of the magnet...

  12. Nuclei acid structure | EMSL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    conditions showed a temporary pH decrease, with a concomitant increase in formic acid during exponential growth. Fermentation experiments performed outside of the magnet...

  13. Recovery of organic acids

    DOE Patents [OSTI]

    Verser, Dan W. (Menlo Park, CA); Eggeman, Timothy J. (Lakewood, CO)

    2011-11-01T23:59:59.000Z

    A method is disclosed for the recovery of an organic acid from a dilute salt solution in which the cation of the salt forms an insoluble carbonate salt. A tertiary amine and CO.sub.2 are introduced to the solution to form the insoluble carbonate salt and a complex between the acid and an amine. A water immiscible solvent, such as an alcohol, is added to extract the acid/amine complex from the dilute salt solution to a reaction phase. The reaction phase is continuously dried and a product between the acid and the solvent, such as an ester, is formed.

  14. Recovery of organic acids

    DOE Patents [OSTI]

    Verser, Dan W. (Golden, CO); Eggeman, Timothy J. (Lakewood, CO)

    2009-10-13T23:59:59.000Z

    A method is disclosed for the recovery of an organic acid from a dilute salt solution in which the cation of the salt forms an insoluble carbonate salt. A tertiary amine and CO.sub.2 are introduced to the solution to form the insoluble carbonate salt and a complex between the acid and an amine. A water immiscible solvent, such as an alcohol, is added to extract the acid/amine complex from the dilute salt solution to a reaction phase. The reaction phase is continuously dried and a product between the acid and the solvent, such as an ester, is formed.

  15. Reversible Acid Gas Capture

    ScienceCinema (OSTI)

    Dave Heldebrant

    2012-12-31T23:59:59.000Z

    Pacific Northwest National Laboratory scientist David Heldebrant demonstrates how a new process called reversible acid gas capture works to pull carbon dioxide out of power plant emissions.

  16. Nmr Study of Thiocarbonyl Derivatives of Fe and Mn were made with solutions of the tungsten and molybdenum species

    E-Print Network [OSTI]

    Bodner, George M.

    Nmr Study of Thiocarbonyl Derivatives of Fe and Mn were made with solutions of the tungsten.g.. the tungsten ion readily oxidizes in aqueous acid [A. Samotus and B. Kosowicz-Czajkowska, Rocz. Chem., 45, 1623

  17. Complex higher order derivative theories

    SciTech Connect (OSTI)

    Margalli, Carlos A.; Vergara, J. David [Instituto de Ciencias Nucleares, Universidad Nacional Autonoma de Mexico, Apartado Postal 70-543, Mexico 04510 DF (Mexico)

    2012-08-24T23:59:59.000Z

    In this work is considered a complex scalar field theory with higher order derivative terms and interactions. A procedure is developed to quantize consistently this system avoiding the presence of negative norm states. In order to achieve this goal the original real scalar high order field theory is extended to a complex space attaching a complex total derivative to the theory. Next, by imposing reality conditions the complex theory is mapped to a pair of interacting real scalar field theories without the presence of higher derivative terms.

  18. Process for producing peracids from aliphatic hydroxy carboxylic acids

    DOE Patents [OSTI]

    Chum, H.L.; Palasz, P.D.; Ratcliff, M.A.

    1984-12-20T23:59:59.000Z

    A process is described for producing peracids from lactic acid-containing solutions derived from biomass processing systems. It consists of adjusting the pH of the solution to about 8 to 9 and removing alkaline residue fractions therefrom to form a solution comprised substantially of lower aliphatic hydroxy acids. The solution is oxidized to produce volatile lower aliphatic aldehydes. The aldehydes are removed as they are generated and converted to peracids.

  19. Materials and methods for efficient lactic acid production

    DOE Patents [OSTI]

    Zhou, Shengde; Ingram, Lonnie O'Neal; Shanmugam, Keelnatham T; Yomano, Lorraine; Grabar, Tammy B; Moore, Jonathan C

    2013-04-23T23:59:59.000Z

    The present invention provides derivatives of Escherichia coli constructed for the production of lactic acid. The transformed E. coli of the invention are prepared by deleting the genes that encode competing pathways followed by a growth-based selection for mutants with improved performance. These transformed E. coli are useful for providing an increased supply of lactic acid for use in food and industrial applications.

  20. SYNTHESIS OF METHACRYLATES FROM COAL-DERIVED SYNGAS

    SciTech Connect (OSTI)

    Jang, B.W.L.; Spivey, J.J.; Gogate, M.R.; Zoeller, J.R.; Colberg, R.D.; Choi, G.N.

    1999-12-01T23:59:59.000Z

    Research Triangle Institute (RTI), Eastman Chemical Company, and Bechtel have developed a novel process for synthesis of methyl methacrylate (MMA) from coal-derived syngas, under a contract from the US Department of Energy/Fossil Energy Technology Center (DOE/FETC). This project has resulted in five US patents (four already published and one pending publication). It has served as the basis for the technical and economic assessment of the production of this high-volume intermediate from coal-derived synthesis gas. The three-step process consists of the synthesis of a propionate from ethylene carbonylation using coal-derived CO, condensation of the propionate with formaldehyde to form methacrylic acid (MAA); and esterification of MAA with methanol to yield MMA. The first two steps, propionate synthesis and condensation catalysis, are the key technical challenges and the focus of the research presented here.

  1. LONG-CHAIN POLYUNSATURATED FATTY ACID INTAKE AND ITS RELATIONSHIP TO RED BLOOD CELL AND SERUM LONG-CHAIN POLYUNSATURATED FATTY ACIDS IN WOMEN AT HIGH RISK FOR BREAST CANCER

    E-Print Network [OSTI]

    Harvey, Katherine

    2010-04-27T23:59:59.000Z

    questionnaire (DHQ). The mean age of the subjects was 47 ± 9.9 years, and the mean body mass index (BMI) was 25 ± 4.4. The mean 5-year Gail risk was 2.7 ± 2.2%. Twenty-two (48%) of the subjects were premenopausal and 24 (52%) were postmenopausal. Fifteen (33...

  2. Solubility of Small-Chain Carboxylic Acids in Supercritical Carbon Dioxide

    SciTech Connect (OSTI)

    Sparks, Darrell L.; Estevez, L. Antonio; Hernandez, Rafael; McEwen, Jason; French, Todd

    2010-07-08T23:59:59.000Z

    The solubility of heptanoic acid and octanoic acid in supercritical carbon dioxide has been determined at temperatures of (313.15, 323.15, and 333.15) K over a pressure range of (8.5 to 30.0) MPa, depending upon the solute. The solubility of heptanoic acid ranged from a solute concentration of (0.08 ± 0.03) kg�m -3 (T = 323.15 K, p = 8.5 MPa) to (147 ± 0.2) kg�m -3 (T = 323.15 K, p = 20.0 MPa). The lowest octanoic acid solubility obtained was a solute concentration of (0.40 ± 0.1) kg�m -3 (T = 333.15 K, p = 10.0 MPa), while the highest solute concentration was (151 ± 2) kg�m -3 (T = 333.15 K, p = 26.7 MPa). Additionally, solubility experiments were performed for nonanoic acid in supercritical carbon dioxide at 323.15 K and pressures of (10.0 to 30.0) MPa to add to the solubility data previously published by the authors. In general, carboxylic acid solubility increased with increasing solvent density. The results also showed that the solubility of the solutes decreased with increasing molar mass at constant supercritical-fluid density. Additionally, the efficacy of Chrastil's equation and other density-based models was evaluated for each fatty acid.

  3. Solubility of Small-Chain Carboxylic Acids in Supercritical Carbon Dioxide

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Sparks, Darrell L.; Estevez, L. Antonio; Hernandez, Rafael; McEwen, Jason; French, Todd

    2010-11-11T23:59:59.000Z

    The solubility of heptanoic acid and octanoic acid in supercritical carbon dioxide has been determined at temperatures of (313.15, 323.15, and 333.15) K over a pressure range of (8.5 to 30.0) MPa, depending upon the solute. The solubility of heptanoic acid ranged from a solute concentration of (0.08 ± 0.03) kg • m -3 (T = 323.15 K, p = 8.5 MPa) to (147 ± 0.2) kg • m -3 (T = 323.15 K, p = 20.0 MPa). The lowest octanoic acid solubility obtained was a solute concentration of (0.40 ± 0.1) kg • m -3 (T = 333.15 K,more »p = 10.0 MPa), while the highest solute concentration was (151 ± 2) kg • m -3 (T = 333.15 K, p = 26.7 MPa). Additionally, solubility experiments were performed for nonanoic acid in supercritical carbon dioxide at 323.15 K and pressures of (10.0 to 30.0) MPa to add to the solubility data previously published by the authors. In general, carboxylic acid solubility increased with increasing solvent density. The results also showed that the solubility of the solutes decreased with increasing molar mass at constant supercritical-fluid density. Additionally, the efficacy of Chrastil's equation and other density-based models was evaluated for each fatty acid.« less

  4. Solubility of Small-Chain Carboxylic Acids in Supercritical Carbon Dioxide

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Sparks, Darrell L.; Estevez, L. Antonio; Hernandez, Rafael; McEwen, Jason; French, Todd

    2010-11-11T23:59:59.000Z

    The solubility of heptanoic acid and octanoic acid in supercritical carbon dioxide has been determined at temperatures of (313.15, 323.15, and 333.15) K over a pressure range of (8.5 to 30.0) MPa, depending upon the solute. The solubility of heptanoic acid ranged from a solute concentration of (0.08 ± 0.03) kg • m -3 (T = 323.15 K, p = 8.5 MPa) to (147 ± 0.2) kg • m -3 (T = 323.15 K, p = 20.0 MPa). The lowest octanoic acid solubility obtained was a solute concentration of (0.40 ± 0.1) kg • m -3 (T = 333.15 K, p = 10.0 MPa), while the highest solute concentration was (151 ± 2) kg • m -3 (T = 333.15 K, p = 26.7 MPa). Additionally, solubility experiments were performed for nonanoic acid in supercritical carbon dioxide at 323.15 K and pressures of (10.0 to 30.0) MPa to add to the solubility data previously published by the authors. In general, carboxylic acid solubility increased with increasing solvent density. The results also showed that the solubility of the solutes decreased with increasing molar mass at constant supercritical-fluid density. Additionally, the efficacy of Chrastil's equation and other density-based models was evaluated for each fatty acid.

  5. Controlling acid rain

    E-Print Network [OSTI]

    Fay, James A.

    1983-01-01T23:59:59.000Z

    High concentrations of sulfuric and nitric acid in raTn fn the northeastern USA are caused by the large scale combustion of fossil fuels within this region. Average precipitation acidity is pH 4.2, but spatial and temporal ...

  6. Effect of dietary polyunsaturated fatty acid and related nutrients on plasma lipids, and skin and hair coat condition in canines

    E-Print Network [OSTI]

    Hester, Shaleah Lynnae

    2004-11-15T23:59:59.000Z

    A study was performed to investigate the effect of diet modifications on skin and hair coat condition in canines. The study included 24 normal adult dogs fed a baseline diet (Ol'Roy[trademark]), during an acclimation period of 12 wk (Phase I). Nine...

  7. Bioaccumulation patterns of methyl mercury and essential fatty acids in lacustrine planktonic food webs and fishB

    E-Print Network [OSTI]

    Mazumder, Asit

    Program, Department of Biology, University of Victoria, P.O. Box 3020, Stn CSC, Victoria (BC) V8W 3N5 Canada b School of Earth and Ocean Sciences, University of Victoria, P.O. Box 3055 STN CSC Victoria (BC) V8W 3P6 Canada Received 13 October 2004; received in revised form 5 August 2005; accepted 12

  8. The response of fatty acids and pigments to variations in temperature and irradiance in the Marine Diatom Thalassiosira pseudonana :

    E-Print Network [OSTI]

    Shang, Frank F.

    2011-01-01T23:59:59.000Z

    Oliveria Luisa Gouveia. Microalgae as a raw materal forunique promise. Microalgae are efficient photoautotrophicto combat global warming. Microalgae embody an exceptionally

  9. Impact of Herbicides on Winter Canola (Brassica napus L.) Production and Fatty Acid Composition in South Texas

    E-Print Network [OSTI]

    Cogdill, Todd Joseph

    2013-01-16T23:59:59.000Z

    DAE was unacceptable for saflufenacil at both 0.12 and 0.06 kg ai ha-1 and ethalfluralin at 1.05 kg ai ha-1. Trifluralin at 1.12 and 0.56 kg ai ha-1, S-metolachlor at 2.14 and 1.07 kg ai ha-1, pyroxasulfone at 0.24 and 0.12 kg ai ha-1...

  10. Effect of dietary polyunsaturated fatty acid and related nutrients on plasma lipids, and skin and hair coat condition in canines 

    E-Print Network [OSTI]

    Hester, Shaleah Lynnae

    2004-11-15T23:59:59.000Z

    A study was performed to investigate the effect of diet modifications on skin and hair coat condition in canines. The study included 24 normal adult dogs fed a baseline diet (Ol'Roy[trademark]), during an acclimation period of 12 wk (Phase I). Nine...

  11. The influence of quality grade, fat trim level and degree of doneness on the fatty acid composition of beef

    E-Print Network [OSTI]

    Harberson, Tish Joan

    1988-01-01T23:59:59.000Z

    in dietary fat rather than the percentage of calories from fat are of utmost importance in effecting serum cholesterol was studied by Hegsted et al. , (1965). Becker et al. (1983) demonstrated that a polyunsaturated fat diet had a lowering effect on both... a b c Means in the same row beanng a common superscript letter are not different (P & 0. 05). d Fat trim levels reported in cm. 23 beneficial in lowering serum LDL-C and leaving HDL-C constant (Becker et al. , 1983). Thus, the large percentage...

  12. Association of Dietary, Circulating, and Supplement Fatty Acids With Coronary Risk: A Systematic Review and Meta-analysis

    E-Print Network [OSTI]

    Chowdhury, Rajiv; Warnakula, Samantha; Kunutsor, Setor; Crowe, Francesca; Ward, Heather A.; Johnson, Laura; Franco, Oscar H.; Butterworth, Adam; Forouhi, Nita G.; Thompson, Simon G.; Khaw, Kay-Tee; Mozaffarian, Dariush; Danesh, John; Di Angelantonio, Emanuele

    2014-03-18T23:59:59.000Z

    Franco5, MD, PhD; Adam Butterworth1, PhD; Nita G Forouhi6, PhD; Simon G Thompson1, FMedSci; Kay-Tee Khaw1, FMedSci; Dariush Mozaffarian7, MD, DrPH; John Danesh1*, FRCP; Emanuele Di Angelantonio1*, MD, MSc, PhD. 1 Department of Public Health... Strangeways Research Laboratory Cambridge, CB1 8RN, England Mailing Address of the authors: Rajiv Chowdhury, Samantha Warnakula, Setor Kunutsor, Adam Butterworth, Kay-Tee Khaw, Simon G Thompson, John Danesh and Emanuele Di Angelantonio: Department...

  13. Effect of Omega 3 Polyunsaturated Fatty Acids (PUFAs) on Markers of Inflammation in Young Horses in Training

    E-Print Network [OSTI]

    Lucia, Jessica Lauren

    2011-02-22T23:59:59.000Z

    was obtained from right radial carpal joint by arthrocentesis every 28 d and was analyzed for white blood cell count (WBC), total protein (TP), and specific gravity (SG). Serum concentrations of carboxypeptide type II collagen (CPII) and chondroitin sulfate 846...

  14. Engineering Bacteria for Efficient Fuel Production: Novel Biological Conversion of Hydrogen and Carbon Dioxide Directly into Free Fatty Acids

    SciTech Connect (OSTI)

    None

    2010-07-12T23:59:59.000Z

    Electrofuels Project: OPX Biotechnologies is engineering a microorganism currently used in industrial biotechnology to directly produce a liquid fuel from hydrogen and carbon dioxide (CO2). The microorganism has the natural ability to use hydrogen and CO2 for growth. OPX Biotechnologies is modifying the microorganism to divert energy and carbon away from growth and towards the production of liquid fuels in larger, commercially viable quantities. The microbial system will produce a fuel precursor that can be chemically upgraded to various hydrocarbon fuels.

  15. PHOSPHOLIPID AND TRIACYLGLYCEROL FATTY ACIDS IN BLOOD AND BREAST TISSUE OBTAINED FROM WOMEN AT INCREASED RISK OF BREAST CANCER

    E-Print Network [OSTI]

    Li, Shengqi

    2010-06-08T23:59:59.000Z

    of PL and TAG in breast tissue were 0.36 ± 0.27% and 1.28 ± 0.44%, respectively. The means of total n-6 PUFA of PL in plasma and RBCs for the subjects were 34.04 ± 3.22% and 28.10 ± 3.45%, respectively, and in plasma TAG was 28.35 ± 3.97%. The means...

  16. Cell, Vol. 119, 889900, December 17, 2004, Copyright 2004 by Cell Press Specific Polyunsaturated Fatty Acids Drive

    E-Print Network [OSTI]

    Schafer, William R.

    that a subset Runnels et al., 2002), prostaglandins (Alessandri-Haber of PUFAs with omega-3 and omega-6 acyl

  17. Prominent bacterial heterotrophy and sources of [superscript 13]C-depleted fatty acids to the interior Canada Basin

    E-Print Network [OSTI]

    Shah, S. R.

    In recent decades, the Canada Basin of the Arctic Ocean has experienced rapidly decreasing summer sea ice coverage and freshening of surface waters. It is unclear how these changes translate to deeper waters, particularly ...

  18. Organ-Specific Alterations in Fatty Acid De Novo Synthesis and Desaturation in a Rat Model of Programmed Obesity

    E-Print Network [OSTI]

    Yee, Jennifer K; Lee, Wai-Nang P; Han, Guang; Ross, Michael G; Desai, Mina

    2011-01-01T23:59:59.000Z

    Prevalence and Trends in Obesity Among US Adults, 1999-2008.C, Barker DJ, Bleker OP: Obesity at the age of 50 y in menDevelopmental programming of obesity in mammals. Exp Physiol

  19. Balancing the benefits of n-3 polyunsaturated fatty acids and the risks of methylmercury exposure from fish consumption

    E-Print Network [OSTI]

    Sunderland, Elsie M.

    capita per day in island states such as Iceland and Japan.1 Consuming fish has many health benefits due from fish consumption Kathryn R Mahaffey, Elsie M Sunderland, Hing Man Chan, Anna L Choi, Philippe documents the latest knowledge on the risks and benefits of seafood consumption for perinatal development

  20. Evaluation of the fatty acid composition and caloric value of ground beef formulated to contain low levels of fat 

    E-Print Network [OSTI]

    Campbell, Laura Elaine

    1988-01-01T23:59:59.000Z

    of the Journal of Food Science. from the bloodstream by attaching to receptor sites located on the liver cells and to a lesser extent at extrahepatic receptor sites. When these receptors become oversaturated with excess LDL's, the LDL's circulate in the plasma... of serum cholesterol and HDL's. However, the high cholesterol diet resulted in an increase in LDL's ar:d a decrease in LDL receptor activity. According to the LDL-receptor hypothesis, there is an increased risk of atherosclerosis when LDL levels become...

  1. Effect of consuming ground beef of differing monounsaturated fatty acid content on atherosclerotic cardiovascular disease risk factors in healthy men 

    E-Print Network [OSTI]

    Cao, Xiaojuan

    2009-05-15T23:59:59.000Z

    lipoprotein (LDL) and increases in high density lipoprotein (HDL). LDL oxidation plays a central role in atherosclerosis development as it both initiates and propagates atherosclerosis. HDL is anti-atherogenic as it can attenuate LDL oxidation. HDLs are a...

  2. The response of fatty acids and pigments to variations in temperature and irradiance in the Marine Diatom Thalassiosira pseudonana :

    E-Print Network [OSTI]

    Shang, Frank F.

    2011-01-01T23:59:59.000Z

    microalgae generate lipids, alcohols, hydrogen, and polysaccharides that can all be transformed into fuel [

  3. n-3 Polyunsaturated Fatty Acids Alter Mouse CD4+ T Cell Activation by Modifying the Lipid Bilayer Properties

    E-Print Network [OSTI]

    Hou, Tim Yu-Tien

    2014-12-05T23:59:59.000Z

    phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2], which modulates actin remodeling, is perturbed by n-3 PUFA. Utilizing the transgenic Fat-1 mouse model that synthesizes n-3 PUFA de novo and enriches the plasma membrane with n-3 PUFA, and wild type (WT) mice fed...

  4. n-3 Polyunsaturated Fatty Acids Suppress Mitochondrial Translocation to the Immunological Synapse and Modulate Calcium Signaling in T Cells

    E-Print Network [OSTI]

    Yog, Rajeshwari

    2011-02-22T23:59:59.000Z

    T helper (Th) cell activation is necessary for the adaptive immune response. Formation of an immunological synapse (IS) between Th cells and antigen-presenting cells is the first step in Th cell activation. In vitro studies indicate that formation...

  5. Effects of ?-, ?1-, and ?2-adrenoceptor agonist infusion on plasma non-esterified fatty acids in non-pregnant,

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    Effects of ?-, ?1-, and ?2-adrenoceptor agonist infusion on plasma non concentration. The latter increased when the demand was enhanced by energy restriction. When injected or infused infusion, three multiparous dry non- pregnant Holstein x Friesian cows were underfed at 60 % of maintenance

  6. Characterization and CPDM modeling of volatile fatty acid fermentation with cotton gin trash and chicken manrure as substrates 

    E-Print Network [OSTI]

    Han, Joseph

    2013-02-22T23:59:59.000Z

    With literally tons of biomass produced annually, a process that uses this waste as a feedstock would help reduce the problem of disposal. The MixAlco process is one that does just that. It converts biomass through anaerobic fermentation...

  7. Characterization and CPDM modeling of volatile fatty acid fermentation with cotton gin trash and chicken manrure as substrates

    E-Print Network [OSTI]

    Han, Joseph

    2013-02-22T23:59:59.000Z

    With literally tons of biomass produced annually, a process that uses this waste as a feedstock would help reduce the problem of disposal. The MixAlco process is one that does just that. It converts biomass through anaerobic fermentation...

  8. N-3 Polyunsaturated Fatty Acids Reduce Th17 Differentiation by Decreasing Responsiveness to Interleukin-6 in Mice

    E-Print Network [OSTI]

    Allen, Marilyn Jean

    2014-05-29T23:59:59.000Z

    and overnight incubation in 10 µg/mL rat anti-mouse gp130 (R&D Systems, Minneapolis, MN). Following washing, cells were subsequently incubated with 10 µg/mL Alexa 555 conjugated donkey anti-rat IgG for two hours followed by incubation with 6 µg/mL Alexa 488... for 2 hours followed by a 90 minute transfer onto a polyvinylidene difluoride membrane (Immobilon) at 400 mAmps. A quantity of 0.1 µg/mL rabbit anti-gp130 (Santa Cruz) was applied overnight followed by 0.1 µg/mL peroxidase labeled goat anti-rabbit IgG...

  9. Cryptosporidium parvum: enhancing our understanding of its unique fatty acid metabolism and the elucidation of putative new inhibitors

    E-Print Network [OSTI]

    Fritzler, Jason Michael

    2008-10-10T23:59:59.000Z

    -2-1,3-benzoxadiazol-4-yl)-methyl]amino NCBI National Center for Biotechnology Information NIH National Institutes of Health NTZ Nitazoxanide ORP Oxysterol Binding Protein-Related Protein OSBP Oxysterol Binding Protein PAGE Polyacrylamide Gel...

  10. Indices of stress in exercising horses fed diets containing varying amounts of omega-6 and omega-3 polyunsaturated fatty acids

    E-Print Network [OSTI]

    Howard, Alicia Dawn

    2005-11-01T23:59:59.000Z

    total workload of 11 approximately 4,000 kg:km per day. On d2 and 4 the lope was shortened to 15 min, followed by a prescribed pattern of maneuvers consisting of galloping circles (~390 m/min), spinning and stopping to achieve a total workload... of approximately 4,000 kg:km per day. The pattern began by galloping 4 circles one direction and a change of leads, followed by 4 circles in the opposite direction and a second change of leads. Next, instead of closing this circle, the horses were galloped...

  11. The response of fatty acids and pigments to variations in temperature and irradiance in the Marine Diatom Thalassiosira pseudonana :

    E-Print Network [OSTI]

    Shang, Frank F.

    2011-01-01T23:59:59.000Z

    towards energy production. Marine microalgae generateMicroalgae are efficient photoautotrophic organisms capable of growing rapidly, producing higher energy

  12. Immunosuppressive dietary n-3 polyunsaturated fatty acids differentially modulate costimulatory regulation of murine CD4+ T-cell function

    E-Print Network [OSTI]

    Ly, Lan H.

    2005-02-17T23:59:59.000Z

    composition of T-lymphocytes and other immune cells. We previously observed that down-regulation of proliferation and cytokine synthesis by CD4+ T-cells in mice fed diets rich in n-3 PUFA was dependent on the involvement of CD28, a co-stimulatory molecule...

  13. Determination of Total Lipids as Fatty Acid Methyl Esters (FAME) by in situ Transesterification: Laboratory Analytical Procedure (LAP)

    Office of Scientific and Technical Information (OSTI)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742EnergyOnItem Not Found Item Not Found The itemAIR57451 CleanFOR IMMEDIATE RELEASE April 13,

  14. Derivative

    National Nuclear Security Administration (NNSA)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742EnergyOn AprilA Approved:AdministrationAnalysisDarby/%2AO 474.2 Chg U.S.09 | Nationaldocument

  15. INTERACTION OF A SUB-BITUMINOUS COAL WITH A STRONG ACID AND A STRONG BASE

    E-Print Network [OSTI]

    Seth, M.

    2010-01-01T23:59:59.000Z

    indicate that coal-derived asphaltenes exhibit an acid-baseanyone functional group is asphaltenes is amphoteric. Theseto oil, and 31.6% to asphaltenes) of a bituminous coal when

  16. Sol-gel derived sorbents

    DOE Patents [OSTI]

    Sigman, Michael E.; Dindal, Amy B.

    2003-11-11T23:59:59.000Z

    Described is a method for producing copolymerized sol-gel derived sorbent particles for the production of copolymerized sol-gel derived sorbent material. The method for producing copolymerized sol-gel derived sorbent particles comprises adding a basic solution to an aqueous metal alkoxide mixture for a pH.ltoreq.8 to hydrolyze the metal alkoxides. Then, allowing the mixture to react at room temperature for a precalculated period of time for the mixture to undergo an increased in viscosity to obtain a desired pore size and surface area. The copolymerized mixture is then added to an immiscible, nonpolar solvent that has been heated to a sufficient temperature wherein the copolymerized mixture forms a solid upon the addition. The solid is recovered from the mixture, and is ready for use in an active sampling trap or activated for use in a passive sampling trap.

  17. Nucleic Acid Database: a Repository of Three-Dimensional Information about Nucleic Acids

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Berman, H. M.; Olson, W. K.; Beveridge, D. L.; Westbrook, J.; Gelbin, A.; Demeny, T.; Hsieh, S. H.; Srinivasan, A. R.; Schneider, B.

    The Nucleic Acid Database (NDB) provides 3-D structural information about nucleic acids.  It is a relational database designed to facilitate the easy search for nucleic acid structures using any of the stored primary or derived structural features. Reports can then be created describing any properties of the selected structures and structures may be viewed in several different formats, including the mmCIF format, the NDB Atlas format, the NDB coordinate format, or the PDB coordinate format. Browsing structure images created directly from coordinates in the repository can also be done. More than 7000 structures have been released as of May 2014. This website also includes a number of specialized tools and interfaces. The NDB Project is funded by the National Institutes of Health and has been funded by the National Science Foundation and the Department of Energy in the past.

  18. Effect of acetic acid on lipid accumulation by glucose-fed activated sludge cultures

    SciTech Connect (OSTI)

    Mondala, Andro; Hernandez, Rafael; French, Todd; McFarland, Linda; Sparks, Darrell; Holmes, William; Haque, Monica

    2012-01-01T23:59:59.000Z

    The effect of acetic acid, a lignocellulose hydrolysis by-product, on lipid accumulation by activated sludge cultures grown on glucose was investigated. This was done to assess the possible application of lignocellulose as low-cost and renewable fermentation substrates for biofuel feedstock production. Results: Biomass yield was reduced by around 54% at a 2 g L -1 acetic acid dosage but was increased by around 18% at 10 g L -1 acetic acid dosage relative to the control run. The final gravimetric lipid contents at 2 and 10 g L -1 acetic acid levels were 12.5 ���± 0.7% and 8.8 ���± 3.2% w/w, respectively, which were lower than the control (17.8 ���± 2.8% w/w). However, biodiesel yields from activated sludge grown with acetic acid (5.6 ���± 0.6% w/w for 2 g L -1 acetic acid and 4.2 ���± 3.0% w/w for 10 g L -1 acetic acid) were higher than in raw activated sludge (1-2% w/w). The fatty acid profiles of the accumulated lipids were similar with conventional plant oil biodiesel feedstocks. Conclusions: Acetic acid enhanced biomass production by activated sludge at high levels but reduced lipid production. Further studies are needed to enhance acetic acid utilization by activated sludge microorganisms for lipid biosynthesis.

  19. Magnetic cellulose-derivative structures

    DOE Patents [OSTI]

    Walsh, Myles A. (Falmouth, MA); Morris, Robert S. (Fairhaven, MA)

    1986-09-16T23:59:59.000Z

    Structures to serve as selective magnetic sorbents are formed by dissolving a cellulose derivative such as cellulose triacetate in a solvent containing magnetic particles. The resulting solution is sprayed as a fine mist into a chamber containing a liquid coagulant such as n-hexane in which the cellulose derivative is insoluble but in which the coagulant is soluble or miscible. On contact with the coagulant, the mist forms free-flowing porous magnetic microspheric structures. These structures act as containers for the ion-selective or organic-selective sorption agent of choice. Some sorbtion agents can be incorporated during the manufacture of the structure.

  20. Heterotrophic microbial activity in lake sediments: effects of organic electron donors

    E-Print Network [OSTI]

    Florida, University of

    by competition for fermentation products such as H2, CO2 and acetate. Microorganisms derive energy production and further break down monomers to alcohols, fatty acids, and hydrogen (H2) through fermentation. Alcohols and fatty acids are then further degraded by syntrophic bacteria (secondary ferment- ers

  1. Quaternion Derivatives: The GHR Calculus

    E-Print Network [OSTI]

    Dongpo Xu; Cyrus Jahanchahi; Clive C. Took; Danilo P. Mandic

    2014-09-25T23:59:59.000Z

    Quaternion derivatives in the mathematical literature are typically defined only for analytic (regular) functions. However, in engineering problems, functions of interest are often real-valued and thus not analytic, such as the standard cost function. The HR calculus is a convenient way to calculate formal derivatives of both analytic and non-analytic functions of quaternion variables, however, both the HR and other functional calculus in quaternion analysis have encountered an essential technical obstacle, that is, the traditional product rule is invalid due to the non- commutativity of the quaternion algebra. To address this issue, a generalized form of the HR derivative is proposed based on a general orthogonal system. The so introduced generalization, called the generalized HR (GHR) calculus, encompasses not just the left- and right-hand versions of quaternion derivative, but also enables solutions to some long standing problems, such as the novel product rule, the chain rule, the mean-valued theorem and Taylor's theorem. At the core of the proposed approach is the quaternion rotation, which can naturally be applied to other functional calculi in non-commutative settings. Examples on using the GHR calculus in adaptive signal processing support the analysis.

  2. Amino acid classes and the protein folding problem

    E-Print Network [OSTI]

    Marek Cieplak; Neal S. Holter; Amos Maritan; Jayanth R. Banavar

    2000-10-18T23:59:59.000Z

    We present and implement a distance-based clustering of amino acids within the framework of a statistically derived interaction matrix and show that the resulting groups faithfully reproduce, for well-designed sequences, thermodynamic stability in and kinetic accessibility to the native state. A simple interpretation of the groups is obtained by eigenanalysis of the interaction matrix.

  3. Focus Sheet | Hydrofluoric Acid Health hazards of hydrofluoric acid

    E-Print Network [OSTI]

    Wilcock, William

    Focus Sheet | Hydrofluoric Acid Health hazards of hydrofluoric acid Hydrofluoric acid (HF characterized by weight loss, brittle bones, anemia, and general ill health. Safe use If possible, avoid working to exposures. #12;Focus Sheet | Hydrofluoric Acid Environmental Health and Safety Environmental Programs Office

  4. Beta-alanine/alpha-ketoglutarate aminotransferase for 3-hydroxypropionic acid production

    DOE Patents [OSTI]

    Jessen, Holly Jean (Chanhassen, MN); Liao, Hans H. (Eden Prairie, MN); Gort, Steven John (Apple Valley, MN); Selifonova, Olga V. (Plymouth, MN)

    2011-10-04T23:59:59.000Z

    The present disclosure provides novel beta-alanine/alpha ketoglutarate aminotransferase nucleic acid and protein sequences having increased biological activity. Also provided are cells containing such enzymes, as well as methods of their use, for example to produce malonyl semialdehyde and downstream products thereof, such as 3-hydroxypropionic acid and derivatives thereof.

  5. Beta-alanine/alpha-ketoglutarate aminotransferase for 3-hydroxypropionic acid production

    DOE Patents [OSTI]

    Jessen, Holly Jean; Liao, Hans H; Gort, Steven John; Selifonova, Olga V

    2014-11-18T23:59:59.000Z

    The present disclosure provides novel beta-alanine/alpha ketoglutarate aminotransferase nucleic acid and protein sequences having increased biological activity. Also provided are cells containing such enzymes, as well as methods of their use, for example to produce malonyl semialdehyde and downstream products thereof, such as 3-hydroxypropionic acid and derivatives thereof.

  6. Limited demand seen for scrubber-derived fertilizers

    SciTech Connect (OSTI)

    Not Available

    1985-12-01T23:59:59.000Z

    By-product marketability of scrubber-derived materials to the fertilizer industry will likely make only a small contribution to the acid rain problem. Those who claim the ammonia-based flue-gas materials will have an economic market cite the Bhara process undergoing testing at Indianapolis Power and Light for the removal of nitrogen oxides and sulfur dioxide, but fertilizer spokesmen feel the market will be limited to certain areas in the Midwest and the Pacific Northwest. This would reduce economic benefits. The Ebara material is easier to handle than ammonia, and should have a competitive price.

  7. Asphaltene damage in matrix acidizing

    E-Print Network [OSTI]

    Hinojosa, Roberto Antonio

    1996-01-01T23:59:59.000Z

    REVIEW CONSTRUCTION OF APPARATUS . DESCRIPTION OF CORE SAMPLES DESIGN OF EXPERIMENTAL ACID TREATMENT . . . ACIDIZING RESULTS BRINE SATURATED CORE L1D ACIDIZING RESULTS BRINE/CRUDE OIL SATURATED CORE S2A . . . ACIDIZING RESULTS BRINE/KEROSENE OIL... experiment they used HCl saturated kerosene to test the same crude samples. Deposition occurred with the HCl saturated acid. The authors concluded, though deposition at an interface was preferential, sludge formation did not require an interface. Moore et...

  8. Optical high acidity sensor

    DOE Patents [OSTI]

    Jorgensen, B.S.; Nekimken, H.L.; Carey, W.P.; O`Rourke, P.E.

    1997-07-22T23:59:59.000Z

    An apparatus and method for determining acid concentrations in solutions having acid concentrations of from about 0.1 Molar to about 16 Molar is disclosed. The apparatus includes a chamber for interrogation of the sample solution, a fiber optic light source for passing light transversely through the chamber, a fiber optic collector for receiving the collimated light after transmission through the chamber, a coating of an acid resistant polymeric composition upon at least one fiber end or lens, the polymeric composition in contact with the sample solution within the chamber and having a detectable response to acid concentrations within the range of from about 0.1 Molar to about 16 Molar, a measurer for the response of the polymeric composition in contact with the sample solution, and a comparer of the measured response to predetermined standards whereby the acid molarity of the sample solution within the chamber can be determined. Preferably, a first lens is attached to the end of the fiber optic light source, the first lens adapted to collimate light from the fiber optic light source, and a second lens is attached to the end of the fiber optic collector for focusing the collimated light after transmission through the chamber. 10 figs.

  9. INTRODUCTION CHONDROCYTES DERIVE their primary energy supply

    E-Print Network [OSTI]

    Waldman, Stephen D.

    of glucose (gly- colysis).1,2 As little of the pyruvate produced by glycol- ysis enters the citric acid cycle (Krebs cycle),3,4 pyruvate is converted to lactic acid by lactate dehydrogenase (in 2:1 molar ratio in culture media because they can perform under both slightly acidic and alkaline conditions. However

  10. Electrochemical versus Anionic Oxygenation of Azathymine Derivatives

    E-Print Network [OSTI]

    Hudlicky, Tomas

    carbohydrazide, pyruvic acid, and 3-pyridinecarboxaldehyde.2 However, all attempts at selective reduction hemiaminals 20, 21, and 22, respectively. Hydrolysis of 20 to 2 was accomplished under either acid- or base in the reduction of the aldimine moiety. The acid-catalyzed deprotection and subsequent closure of 5, prepared from

  11. 2 SPRAY OILS--BEYOND 2000 Modern use of petroleum-derived oils as agricultural crop

    E-Print Network [OSTI]

    Agnello, Arthur M.

    ,buttheseweretoophytotoxic.Eventually, researchersconcentratedondistillatesintherangebetween kerosene and lubricating oils.Three basic classes of carbon structures present in petroleum oils2 SPRAY OILS--BEYOND 2000 Abstract Modern use of petroleum-derived oils as agricultural crop (aromatics and other un- saturated components) in oils that were removable with sulfuric acid; the remainder

  12. Synthesis of acid addition salt of delta-aminolevulinic acid from 5-bromo levulinic acid esters

    DOE Patents [OSTI]

    Moens, Luc (Lakewood, CO)

    2003-06-24T23:59:59.000Z

    A process of preparing an acid addition salt of delta-aminolevulinc acid comprising: a) dissolving a lower alkyl 5-bromolevulinate and hexamethylenetetramine in a solvent selected from the group consisting of water, ethyl acetate, chloroform, acetone, ethanol, tetrahydrofuran and acetonitrile, to form a quaternary ammonium salt of the lower alkyl 5-bromolevulinate; and b) hydrolyzing the quaternary ammonium salt with an inorganic acid to form an acid addition salt of delta-aminolevulinic acid.

  13. Binder enhanced refuse derived fuel

    DOE Patents [OSTI]

    Daugherty, Kenneth E. (Lewisville, TX); Venables, Barney J. (Denton, TX); Ohlsson, Oscar O. (Naperville, IL)

    1996-01-01T23:59:59.000Z

    A refuse derived fuel (RDF) pellet having about 11% or more particulate calcium hydroxide which is utilized in a combustionable mixture. The pellets are used in a particulate fuel bring a mixture of 10% or more, on a heat equivalent basis, of the RDF pellet which contains calcium hydroxide as a binder, with 50% or more, on a heat equivalent basis, of a sulphur containing coal. Combustion of the mixture is effective to produce an effluent gas from the combustion zone having a reduced SO.sub.2 and polycyclic aromatic hydrocarbon content of effluent gas from similar combustion materials not containing the calcium hydroxide.

  14. Organic derivatives of zirconium phosphate

    E-Print Network [OSTI]

    Fine, Steven Beryl

    1980-01-01T23:59:59.000Z

    -zirconium phosphate derivatives. 9 Thermogravimetric analysis of y-zirconium 2-hydroxy- 1-ethyl phosphate. 25 10 Thermogravimetric analysis of y-zirconium 2-carboxy- 2-hydroxy-1-ethyl phosphate. 28 11 Thermo gravimetric 1-ethyl phosphate. analysis of n...-ZrP Figure 3. A comparison of the layered structures of c and y zirconium phosphate. according to Yamanaka [9], ethylene oxide is 18. 4 A [ 10] . When this phase is dehydrated at 150 for one hour, the d-spacing decreases to 17. 2 A, but when left in air...

  15. Deriving Ontologies from XML Schema

    E-Print Network [OSTI]

    Bedini, Ivan; Nguyen, Benjamin

    2010-01-01T23:59:59.000Z

    In this paper, we present a method and a tool for deriving a skeleton of an ontology from XML schema files. We first recall what an is ontology and its relationships with XML schemas. Next, we focus on ontology building methodology and associated tool requirements. Then, we introduce Janus, a tool for building an ontology from various XML schemas in a given domain. We summarize the main features of Janus and illustrate its functionalities through a simple example. Finally, we compare our approach to other existing ontology building tools.

  16. Biosynthesis of the unique trans-delta 3-hexadecenoic acid component of chloroplast phosphatidylglycerol: Evidence concerning its site and mechanism of formation

    SciTech Connect (OSTI)

    Ohnishi, M.; Thompson, G.A. Jr. (Department of Botany, University of Texas, Austin (United States))

    1991-08-01T23:59:59.000Z

    As in most higher plants, chloroplast membranes of the green alga Dunaliella salina contain phosphatidylglycerol (PG) that is rich in trans-delta 3-hexadecenoic acid (16:1t), a fatty acid found nowhere else in the cell. After labeling D. salina with exogenous (3H)myristic acid (( 3H)14:0), the cis-unsaturated fatty acids of monogalactosyldiacylglycerol and sulfoquinovosyldiacylglycerol as well as PG had higher specific radioactivities in chloroplast envelopes than in thylakoids. In contrast, 16:1t was very slow to become radioactive, and its specific radioactivity was several times higher in isolated thylakoids than in envelopes after brief (3-20 min) labeling with (3H)14:0. Analysis of individual PG molecular species revealed that the fatty acid paired with 16:1t was also labeled slowly. Thus linoleate (18:2) released from a 16:1t-containing PG had a 350-fold (at 3 min) to 20-fold (at 60 min) lower specific radioactivity than did 18:2 from a palmitate (16:0)-containing PG. The findings suggest that the substrates for trans-desaturation are 16:0-containing PG molecular species which are readily labeled from (3H)14:0 in the envelope but are diluted by the large pool of thylakoid PG before penetrating to the desaturation site. By examining the labeling patterns of individual PG molecular species classes, it was concluded that D. salina 16:1t is formed from 16:0 linked to 18:2/16:0 PG and 18:3/16:0 PG by a trans-desaturase located within the inner recesses of the thylakoid compartment.

  17. acid succinic acid: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    simulated the laser heating of the succinic acid (this data is still simulation is that infrared heating generates about 10-15 more succinic acid molecules bound to the analyte...

  18. acid docosahexaenoic acid: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    by USDA and U of I researchers Illinois at Urbana-Champaign, University of 38 Controlling acid rain MIT - DSpace Summary: High concentrations of sulfuric and nitric acid in raTn fn...

  19. acid aspartic acid: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    by USDA and U of I researchers Illinois at Urbana-Champaign, University of 20 Controlling acid rain MIT - DSpace Summary: High concentrations of sulfuric and nitric acid in raTn fn...

  20. acid caffeic acid: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    by USDA and U of I researchers Illinois at Urbana-Champaign, University of 11 Controlling acid rain MIT - DSpace Summary: High concentrations of sulfuric and nitric acid in raTn fn...

  1. acid propionic acid: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    by USDA and U of I researchers Illinois at Urbana-Champaign, University of 19 Controlling acid rain MIT - DSpace Summary: High concentrations of sulfuric and nitric acid in raTn fn...

  2. acid sorbic acid: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    by USDA and U of I researchers Illinois at Urbana-Champaign, University of 9 Controlling acid rain MIT - DSpace Summary: High concentrations of sulfuric and nitric acid in raTn fn...

  3. acid benzoic acid: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    by USDA and U of I researchers Illinois at Urbana-Champaign, University of 24 Controlling acid rain MIT - DSpace Summary: High concentrations of sulfuric and nitric acid in raTn fn...

  4. acid propanoic acid: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    by USDA and U of I researchers Illinois at Urbana-Champaign, University of 9 Controlling acid rain MIT - DSpace Summary: High concentrations of sulfuric and nitric acid in raTn fn...

  5. acid methoxyacetic acid: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    by USDA and U of I researchers Illinois at Urbana-Champaign, University of 7 Controlling acid rain MIT - DSpace Summary: High concentrations of sulfuric and nitric acid in raTn fn...

  6. acid eicosapentaenoic acid: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    by USDA and U of I researchers Illinois at Urbana-Champaign, University of 18 Controlling acid rain MIT - DSpace Summary: High concentrations of sulfuric and nitric acid in raTn fn...

  7. acids eicosapentaenoic acid: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    by USDA and U of I researchers Illinois at Urbana-Champaign, University of 18 Controlling acid rain MIT - DSpace Summary: High concentrations of sulfuric and nitric acid in raTn fn...

  8. acid acetylsalicylic acid: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    by USDA and U of I researchers Illinois at Urbana-Champaign, University of 10 Controlling acid rain MIT - DSpace Summary: High concentrations of sulfuric and nitric acid in raTn fn...

  9. acid dichloroacetic acid: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    by USDA and U of I researchers Illinois at Urbana-Champaign, University of 7 Controlling acid rain MIT - DSpace Summary: High concentrations of sulfuric and nitric acid in raTn fn...

  10. acid oleic acid: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    by USDA and U of I researchers Illinois at Urbana-Champaign, University of 31 Controlling acid rain MIT - DSpace Summary: High concentrations of sulfuric and nitric acid in raTn fn...

  11. Recovery of Carboxylic Acids from Fermentation Broth via Acid Springing

    E-Print Network [OSTI]

    Dong, Jipeng

    2010-01-14T23:59:59.000Z

    RECOVERY OF CARBOXYLIC ACIDS FROM FERMENTATION BROTH VIA ACID SPRINGING A Thesis by JIPENG DONG Submitted to the Office of Graduate Studies of Texas A&M University in partial fulfillment of the requirements for the degree... of MASTER OF SCIENCE December 2008 Major Subject: Chemical Engineering RECOVERY OF CARBOXYLIC ACIDS FROM FERMENTATION BROTH VIA ACID SPRINGING A Thesis by JIPENG DONG Submitted to the Office of Graduate Studies of Texas A...

  12. Acid placement and coverage in the acid jetting process

    E-Print Network [OSTI]

    Mikhailov, Miroslav I.

    2009-05-15T23:59:59.000Z

    Many open-hole acid treatments are being conducted by pumping acid through jetting ports placed at the end of coiled tubing or drill pipe. The filter-cake on the bore-hole is broken by the jet; the acid-soluble material is dissolved, creating...

  13. Lubrication with boric acid additives

    DOE Patents [OSTI]

    Erdemir, Ali (Naperville, IL)

    2000-01-01T23:59:59.000Z

    Self-lubricating resin compositions including a boric acid additive and a synthetic polymer including those thermoset materials.

  14. Mutant E. coli strain with increased succinic acid production

    DOE Patents [OSTI]

    Donnelly, M.; Millard, C.S.; Stols, L.

    1998-06-23T23:59:59.000Z

    A method for isolating succinic acid producing bacteria is provided comprising increasing the biomass of an organism which lacks the ability to catabolize pyruvate, and then subjecting the biomass to glucose-rich medium in an anaerobic environment to enable pyruvate-catabolizing mutants to grow. The invention also provides for a mutant that produces high amounts of succinic acid, which as been derived from a parent which lacked the genes for pyruvate formate lyase and lactate dehydrogenase, and which belongs to the E.coli Group of Bacteria. 2 figs.

  15. Mutant E. coli strain with increased succinic acid production

    DOE Patents [OSTI]

    Donnelly, Mark; Millard, Cynthia S.; Stols, Lucy

    2001-09-25T23:59:59.000Z

    A method for isolating succinic acid producing bacteria is provided comprising increasing the biomass of an organism which lacks the ability to catabolize pyruvate, and then subjecting the biomass to glucose-rich medium in an anaerobic environment to enable pyruvate-catabolizing mutants to grow. The invention also provides for a mutant that produces high amounts of succinic acid, which has been derived from a parent which lacked the genes for pyruvate formate lyase and lactate dehydrogenase, and which belongs to the E.coli Group of Bacteria.

  16. Mutant E. coli strain with increased succinic acid production

    DOE Patents [OSTI]

    Donnelly, Mark (Warrenville, IL); Millard, Cynthia S. (Plainfield, IL); Stols, Lucy (Woodridge, IL)

    2002-01-01T23:59:59.000Z

    A method for isolating succinic acid producing bacteria is provided comprising increasing the biomass of an organism which lacks the ability to catabolize pyruvate, and then subjecting the biomass to glucose-rich medium in an anaerobic environment to enable pyruvate-catabolizing mutants to grow. The invention also provides for a mutant that produces high amounts of succinic acid, which has been derived from a parent which lacked the genes for pyruvate formate lyase and lactate dehydrogenase, and which belongs to the E.coli Group of Bacteria.

  17. Mutant E. coli strain with increased succinic acid production

    DOE Patents [OSTI]

    Donnelly, Mark (Warrenville, IL); Millard, Cynthia S. (Plainfield, IL); Stols, Lucy (Woodridge, IL)

    1998-01-01T23:59:59.000Z

    A method for isolating succinic acid producing bacteria is provided comprising increasing the biomass of an organism which lacks the ability to catabolize pyruvate, and then subjecting the biomass to glucose-rich medium in an anaerobic environment to enable pyruvate-catabolizing mutants to grow. The invention also provides for a mutant that produces high amounts of succinic acid, which as been derived from a parent which lacked the genes for pyruvate formate lyase and lactate dehydrogenase, and which belongs to the E.coli Group of Bacteria.

  18. Pantothenic acid biosynthesis in zymomonas

    DOE Patents [OSTI]

    Tao, Luan; Tomb, Jean-Francois; Viitanen, Paul V.

    2014-07-01T23:59:59.000Z

    Zymomonas is unable to synthesize pantothenic acid and requires this essential vitamin in growth medium. Zymomonas strains transformed with an operon for expression of 2-dehydropantoate reductase and aspartate 1-decarboxylase were able to grow in medium lacking pantothenic acid. These strains may be used for ethanol production without pantothenic acid supplementation in seed culture and fermentation media.

  19. Investigating acid rain

    SciTech Connect (OSTI)

    Not Available

    1981-01-01T23:59:59.000Z

    A report is given of an address by Kathleen Bennett, Assistant Administrator of Air, Noise and Radiation, Environmental Protection Agency which was presented to the US Senate Committee on the Environment and Public Works. Bennet explained that in view of the many unknowns about acid rain, and the possible substantial cost burden of additional controls, EPA is proceeding with its program to investigate this environmental malady over a 10-year period. The three major areas of the research program are (1) transport, transformation, and deposition processes, (2) effects of acid deposition, and (3) assessments and policy studies. Other issues discussed were global transboundary air pollution and Senate amendments addressing long-range transport. (JMT)

  20. Introduction Aliphatic polyesters derived from renewable

    E-Print Network [OSTI]

    Introduction Aliphatic polyesters derived from renewable resources are of increasing interest, there are also opportunities to derive lactones from biomass, which can then be converted to a wide range

  1. A study on real estate derivatives

    E-Print Network [OSTI]

    Lim, Jong Yoon, S.M. Massachusetts Institute of Technology

    2006-01-01T23:59:59.000Z

    All major asset classes including stocks and bonds have a well developed derivative market. Derivatives enable counterparties to reflect a view on a particular market, without having to trade the underlying asset. This ...

  2. The Fourth Partial Derivative In Transport Dynamics

    E-Print Network [OSTI]

    Trinh Khanh Tuoc

    2010-01-11T23:59:59.000Z

    A new fourth partial derivative is introduced for the study of transport dynamics. It is a Lagrangian partial derivative following the path of diffusion, not the path of convection. Use of this derivative decouples the effect of diffusion and convection and simplifies the analysis of transport processes.

  3. Postgraduate Scholarship Pricing temperature derivatives and modelling

    E-Print Network [OSTI]

    Banaji,. Murad

    the volumetric risk of the energy units sold, rather than the price risk of each unit. Weather derivativesPostgraduate Scholarship Pricing temperature derivatives and modelling the market price of risk: Pricing temperature derivatives and modelling the market price of risk. Main Supervisor: A. Alexandridis

  4. Synthesis of acrylates and Methacrylates from Coal-Derived Syngas

    SciTech Connect (OSTI)

    NONE

    1997-05-12T23:59:59.000Z

    Research Triangle Institute (RTI), Eastman Chemical Company, and Bechtel collectively are developing a novel process for the synthesis of methyl methacrylate (MMA) from coal-derived syngas, under a contract from the U.S. Department of Energy, Federal Energy Technology Center. This three-step process consists of synthesis of a propionate, its condensation with formaldehyde, and esterification of resulting methacrylic acid (MAA) with methanol to produce MMA. Eastman has focused on the propionate synthesis step. The resultant Mo catalysts work efficiently at much less severe conditions (170{degrees}C and 30 atm) than the conventional Ni catalysts (270{degrees} C and 180 atm). Bechtel has performed an extensive cost analysis which shows that Eastman`s propionate synthesis step is competitive with other technologies to produce the anhydride. Eastman and Bechtel have also compared the RTI- Eastman-Bechtel three-step methanol route to five other process routes to MMA. The results show that the product MMA can be produced at 520/lb, for a 250 Mlb/year MMA plant, and this product cost is competitive to all other process routes to MMA, except propyne carbonylation. In the second step, RTI and Eastman have developed active and stable V-SI-P tertiary metal oxide catalysts, Nb/Si0{sub 2}, and Ta/Si0{sub 2} catalysts for condensation of propionic anhydride or propionic acid with formaldehyde. RTI has demonstrated a novel correlation among the catalyst acid-base properties, condensation reaction yield, and long-term catalyst performance. Eastman and Bechtel have used the RTI experimental results of a 20 percent Nb/Si0{sub 2} catalyst, in terms of reactant conversions, MAA selectivities, and MAA yield, for their economic analysis. Recent research focuses on enhancing the condensation reaction yields, a better understanding of the acid-base property correlation and enhancing the catalyst lifetime.

  5. Invasive cleavage of nucleic acids

    DOE Patents [OSTI]

    Prudent, James R. (Madison, WI); Hall, Jeff G. (Madison, WI); Lyamichev, Victor I. (Madison, WI); Brow, Mary Ann D. (Madison, WI); Dahlberg, James E. (Madison, WI)

    2002-01-01T23:59:59.000Z

    The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

  6. Total Acid Value Titration of Hydrotreated Biomass Fast Pyrolysis Oil: Determination of Carboxylic Acids and Phenolics with Multiple End-Point Detection

    SciTech Connect (OSTI)

    Christensen, E.; Alleman, T. L.; McCormick, R. L.

    2013-01-01T23:59:59.000Z

    Total acid value titration has long been used to estimate corrosive potential of petroleum crude oil and fuel oil products. The method commonly used for this measurement, ASTM D664, utilizes KOH in isopropanol as the titrant with potentiometric end point determination by pH sensing electrode and Ag/AgCl reference electrode with LiCl electrolyte. A natural application of the D664 method is titration of pyrolysis-derived bio-oil, which is a candidate for refinery upgrading to produce drop in fuels. Determining the total acid value of pyrolysis derived bio-oil has proven challenging and not necessarily amenable to the methodology employed for petroleum products due to the different nature of acids present. We presented an acid value titration for bio-oil products in our previous publication which also utilizes potentiometry using tetrabutylammonium hydroxide in place of KOH as the titrant and tetraethylammonium bromide in place of LiCl as the reference electrolyte to improve the detection of these types of acids. This method was shown to detect numerous end points in samples of bio-oil that were not detected by D664. These end points were attributed to carboxylic acids and phenolics based on the results of HPLC and GC-MS studies. Additional work has led to refinement of the method and it has been established that both carboxylic acids and phenolics can be determined accurately. Use of pH buffer calibration to determine half-neutralization potentials of acids in conjunction with the analysis of model compounds has allowed us to conclude that this titration method is suitable for the determination of total acid value of pyrolysis oil and can be used to differentiate and quantify weak acid species. The measurement of phenolics in bio-oil is subject to a relatively high limit of detection, which may limit the utility of titrimetric methodology for characterizing the acidic potential of pyrolysis oil and products.

  7. Alternative Fuels Data Center

    Alternative Fuels and Advanced Vehicles Data Center [Office of Energy Efficiency and Renewable Energy (EERE)]

    Biodiesel Definition and Specification Biodiesel is defined as a fuel that is comprised of mono-alkyl esters of long-chain fatty acids derived from vegetable oils or animal fats...

  8. Alternative Fuels Data Center

    Alternative Fuels and Advanced Vehicles Data Center [Office of Energy Efficiency and Renewable Energy (EERE)]

    Biodiesel Producer Requirements Biodiesel is defined as a fuel that is composed of mono-alkyl esters of long-chain fatty acids derived from plant or animal matter, meets the...

  9. Alternative Fuels Data Center

    Alternative Fuels and Advanced Vehicles Data Center [Office of Energy Efficiency and Renewable Energy (EERE)]

    Biodiesel Definition Biodiesel is a renewable special fuel that is composed of mono-alkyl esters of long chain fatty acids, derived from vegetable oils or animal fats, and meets...

  10. Alternative Fuels Data Center

    Alternative Fuels and Advanced Vehicles Data Center [Office of Energy Efficiency and Renewable Energy (EERE)]

    Biodiesel Definition Biodiesel fuel is defined as a mono alkyl ester of long chain fatty acids derived from vegetable oils or animal fats for use in compression-ignition engines...

  11. Alternative Fuels Data Center

    Alternative Fuels and Advanced Vehicles Data Center [Office of Energy Efficiency and Renewable Energy (EERE)]

    Biodiesel Definition Biodiesel is defined as a fuel comprised of mono-alkyl esters of long chain fatty acids derived from vegetable oils or animal fats, either in pure form or...

  12. Unconventional fuel: Tire derived fuel

    SciTech Connect (OSTI)

    Hope, M.W. [Waste Recovery, Inc., Portland, OR (United States)

    1995-09-01T23:59:59.000Z

    Material recovery of scrap tires for their fuel value has moved from a pioneering concept in the early 1980`s to a proven and continuous use in the United States` pulp and paper, utility, industrial, and cement industry. Pulp and paper`s use of tire derived fuel (TDF) is currently consuming tires at the rate of 35 million passenger tire equivalents (PTEs) per year. Twenty mills are known to be burning TDF on a continuous basis. The utility industry is currently consuming tires at the rate of 48 million PTEs per year. Thirteen utilities are known to be burning TDF on a continuous basis. The cement industry is currently consuming tires at the rate of 28 million PTEs per year. Twenty two cement plants are known to be burning TDF on a continuous basis. Other industrial boilers are currently consuming tires at the rate of 6.5 million PTEs per year. Four industrial boilers are known to be burning TDF on a continuous basis. In total, 59 facilities are currently burning over 117 million PTEs per year. Although 93% of these facilities were not engineered to burn TDF, it has become clear that TDF has found acceptance as a supplemental fuel when blending with conventional fuels in existing combustion devices designed for normal operating conditions. The issues of TDF as a supplemental fuel and its proper specifications are critical to the successful development of this fuel alternative. This paper will focus primarily on TDF`s use in a boiler type unit.

  13. Transpiration and water use by cotton plants as affected by the soil application of fatty alcohols 

    E-Print Network [OSTI]

    Rhoads, Frederick Milton

    1963-01-01T23:59:59.000Z

    on tz snspl: ' icn cont~ oi is almost non-existent for cotton. The reseat'ch zepo 'ted berin is one. pa. -. of a joint resea h program which is being conducred by t?e Texas Agriculcur=l Experimen Station and the Procter and Gamble Company fo. de... the fatty alcohols were incuba ed in moist soil for the pre- scribed length of time, the soil was dried in an oven, pulverized by grind- ing, placed in marked containers, and shipped to lfr. heal Thompson, of The Procter and Gamble Company (Pecos, Texas...

  14. Release of Brain Mitochondrial Hexokinase by Acidic Proteins and Macromolecular Polyanions

    E-Print Network [OSTI]

    F. Moller

    2005-03-22T23:59:59.000Z

    Preparations of arachidonic acid binding and non-binding proteins from bovine brain, four acidic proteins (alpha-casein, phosvitin, beta-lactoglobulin A and B), the peptide polyglutamate, and two polyanions (heparin, dextran sulfate) enhanced both basal and glucose 6-phosphate induced solubilization of rat brain mitochondrial hexokinase (ATP:D-hexose 6-phosphotransferase, EC 2.7.1.1). In contrast, three other acidic proteins, had little (alpha-lactalbumin) or no effect (bovine serum albumin, ovalbumin) and five basic proteins inhibited release of the enzyme. Solubilizing activity therefore appears to require a net negative charge and one or more of the following structural features: extended conformation, random coil, and unordered or beta-structure, in the latter case, as the beta-barrel in the fatty acid binding proteins and beta-lactoglobulins. It is of interest that a difference of a single negative charge between beta-lactoglobulin A and B, resulted in a statistically significant difference in the stimulation of hexokinase release. Possible physiological and pathological roles of this hexokinase solubilizing effect are discussed briefly.

  15. Metabolic evolution of Escherichia coli strains that produce organic acids

    DOE Patents [OSTI]

    Grabar, Tammy; Gong, Wei; Yocum, R Rogers

    2014-10-28T23:59:59.000Z

    This invention relates to the metabolic evolution of a microbial organism previously optimized for producing an organic acid in commercially significant quantities under fermentative conditions using a hexose sugar as sole source of carbon in a minimal mineral medium. As a result of this metabolic evolution, the microbial organism acquires the ability to use pentose sugars derived from cellulosic materials for its growth while retaining the original growth kinetics, the rate of organic acid production and the ability to use hexose sugars as a source of carbon. This invention also discloses the genetic change in the microorganism that confers the ability to use both the hexose and pentose sugars simultaneously in the production of commercially significant quantities of organic acids.

  16. acid acetic acid: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of asphaltene deposition that occurs during acid treatments of oil reservoirs. Asphaltenes are present to some degree in most hydrocarbons. Due to the molecular weight of the...

  17. The effect of dietary fat, caloric intake, and protein level on the development of fatty livers in the mature hen 

    E-Print Network [OSTI]

    McDaniel, Archie Hugh

    1958-01-01T23:59:59.000Z

    fc h. M CUlLcUE Or ] tXA? THE EFFECT OF DIETARY FAT, CALORIC INTAKE, AND PROTEIN LEVEL ON THE DEVELOPMENT OF FATTY LIVERS IN THE MATURE HEN A Dissertation By Archie Hugh McDaniel Submitted to the Graduate School of the Agricultural... and Mechanical College of Texas in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY May 1958 Major Subject: Poultry Science (Nutrition) THE EFFECT OF DIETARY FAT, CALORIC INTAKE, AND PROTEIN LEVEL ON THE DEVELOPMENT OF FATTY...

  18. Mechanistic insights into a BINOL-derived phosphoric acid-catalyzed asymmetric Pictet-Spengler reaction

    E-Print Network [OSTI]

    Overvoorde, Lois M.; Grayson, Matthew N.; Luo, Yi; Goodman, Jonathan M.

    2015-02-05T23:59:59.000Z

    Chemistry Article DOI: 10.1021/jo5028134 J. Org. Chem. 2015, 80, 2634?2640 2635 proton to form 6 (RR), and the formation of this cis-fused product is consistent with the experimental evidence found by Holloway et al.1 2.2. Catalysis. The catalyzed reaction... the full catalyst structure was considered. DFT (B3LYP/6- 31G**) was used for the high layer and MM53 [universal force field (UFF)54] for the low layer, with M06-2X single-point energy correction subsequently applied to the whole structure. The use of ONIOM...

  19. Advanced electrodialysis and pervaporation for fermentation-derived organic acids production.

    SciTech Connect (OSTI)

    Tsai, S. P.

    1998-11-18T23:59:59.000Z

    Lactate esters produced from carbohydrate have potential markets as nontoxic replacements for halogenated and toxic solvents and as feedstocks for large-volume chemicals and polymers. Argonne National Laboratory has developed a novel process for the production of high-purity lactate esters from carbohydrate. The process uses advanced electrodialysis and pervaporation technologies to overcome major technical barriers in product separation; more specifically, the process involves cation elimination without the generation of salt waste and efficient esterification for final purification. This patented process requires little energy input, is highly efficient and selective, eliminates the large volumes of salt waste produced by conventional processes, and significantly reduces manufacturing costs. The enabling membrane separation technologies make it technically and commercially feasible for lactate esters to penetrate the potential markets.

  20. Characterization of ?-carrageenan and its derivative based green polymer electrolytes

    SciTech Connect (OSTI)

    Jumaah, Fatihah Najirah; Mobaraka, Nadhratun Naiim; Ahmad, Azizan; Ramli, Nazaruddin [School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600, Bangi, Selangor Darul Ehsan (Malaysia)

    2013-11-27T23:59:59.000Z

    The new types of green polymer electrolytes based on ?-carrageenan derivative have been prepared. ?-carrageenan act as precursor was reacted with monochloroacetic acid to produce carboxymethyl ?-carrageenan. The powders were characterized by Attenuated Total Reflection Fourier Transform infrared (ATR-FTIR) spectroscopy and {sup 1}H nuclear magnetic resonance (NMR) to confirm the substitution of targeted functional group in ?-carrageenan. The green polymer electrolyte based on ?-carrageenan and carboxymethyl ?-carrageenan was prepared by solution-casting technique. The films were characterized by electrochemical impedance spectroscopy to determine the ionic conductivity. The ionic conductivity ?-carrageenan film were higher than carboxymethyl ?-carrageenan which 4.87 ×10{sup ?6} S cm{sup ?1} and 2.19 ×10{sup ?8} S cm{sup ?1}, respectively.

  1. Analysis of the scent gland secretions of Dumeril's ground boa (Acrantophis dumerili Jan) by gas chromatography-mass spectrometry

    E-Print Network [OSTI]

    Simpson, John Thomas

    1989-01-01T23:59:59.000Z

    -MS). The lipids were extracted from the crude secretions with chloroform and frac- tionated by acid-base extraction or by thin-layer chromatography. Appropriate frac- tions were converted to derivatives with diazomethane or with trimethylsilyl chloride. Total... fatty acid content of the crude secretions was obtained by transesterification to the methyl esters and analysis by GC-MS. The lipid fraction of the scent gland secretions of A. dumerili contain primarily fatty acids and cholesterol. A homologous...

  2. Acidic gas capture by diamines

    DOE Patents [OSTI]

    Rochelle, Gary (Austin, TX); Hilliard, Marcus (Missouri City, TX)

    2011-05-10T23:59:59.000Z

    Compositions and methods related to the removal of acidic gas. In particular, the present disclosure relates to a composition and method for the removal of acidic gas from a gas mixture using a solvent comprising a diamine (e.g., piperazine) and carbon dioxide. One example of a method may involve a method for removing acidic gas comprising contacting a gas mixture having an acidic gas with a solvent, wherein the solvent comprises piperazine in an amount of from about 4 to about 20 moles/kg of water, and carbon dioxide in an amount of from about 0.3 to about 0.9 moles per mole of piperazine.

  3. Calibration by Optimization Without Using Derivatives

    E-Print Network [OSTI]

    Markus Lazar

    2015-03-06T23:59:59.000Z

    Mar 6, 2015 ... Abstract: Applications in engineering frequently require the ... to upper and lower bounds without relying on the knowledge of the derivative of f .

  4. Anisotropic higher derivative gravity and inflationary universe

    E-Print Network [OSTI]

    W. F. Kao

    2006-05-21T23:59:59.000Z

    Stability analysis of the Kantowski-Sachs type universe in pure higher derivative gravity theory is studied in details. The non-redundant generalized Friedmann equation of the system is derived by introducing a reduced one dimensional generalized KS type action. This method greatly reduces the labor in deriving field equations of any complicate models. Existence and stability of inflationary solution in the presence of higher derivative terms are also studied in details. Implications to the choice of physical theories are discussed in details in this paper.

  5. GLOBAL CONVERGENCE OF GENERAL DERIVATIVE-FREE ...

    E-Print Network [OSTI]

    2006-10-26T23:59:59.000Z

    a relatively recent topic [1, 3, 7, 10]. In this paper we address trust-region methods for unconstrained derivative-free optimization. These methods ... Page 2

  6. Organic Phosphoric Acid of the Soil.

    E-Print Network [OSTI]

    Fraps, G. S. (George Stronach)

    1911-01-01T23:59:59.000Z

    . ................................................ introduction 5 .............................. hmmonia-Soluble Phosphoric Acid 5 ................ Solubility of Phosphates in Ammonia 6 I Fixation of Phosphoric Acid from Ammonia .......... 7 Effect of Ratio of Soil to Solvent in Extraction of Phos- I I... .............. phoric Acid by Acid and Ammonia 7 I ........ Other Soil Constituents Dissolved by Ammonia 8 ................... Solution of Fixed Phosphoric Acid 10 ................ ormation of Ammonia-Solubla Phosphoric Acid 11 ....... hosphoric Acid Dissolved...

  7. acidic alpha-amino acids: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    acid indicated that more succinic acid... Gilliland, Patti Lynn 2012-06-07 24 A ACID RAIN Audrey Gibson Geosciences Websites Summary: , oxygen, and oxidants to form...

  8. Soluble epoxide hydrolase regulates hematopoietic progenitor cell function via generation of fatty

    E-Print Network [OSTI]

    Hammock, Bruce D.

    formation), but the sEH products 12,13-dihydroxyoctadecenoic acid (12,13-DiHOME) and 11,12- dihydroxyeicosatrienoic acid stimulated canonical Wnt signaling and rescued the effects of sEH inhibition. In murine bone either the restoration of bone marrow sEH activity or treatment with 12,13-DiHOME. Thus, sEH activity

  9. A Multi-period Equilibrium Pricing Model of Weather Derivatives

    E-Print Network [OSTI]

    Lee, Yongheon; Oren, Shmuel S.

    2008-01-01T23:59:59.000Z

    2002). On modelling and pricing weather derivatives. Applied2003). Arbitrage-fee pricing of weather derivatives based onfects and valuation of weather derivatives. The Financial

  10. A multi-period equilibrium pricing model of weather derivatives

    E-Print Network [OSTI]

    Lee, Yongheon; Oren, Shmuel S.

    2010-01-01T23:59:59.000Z

    Y. : Valuation and hedging of weather derivatives on monthlyJ. Risk 31. Yoo, S. : Weather derivatives and seasonaleffects and valuation of weather derivatives. Financ. Rev.

  11. Superbase-derived protic ionic liquids

    DOE Patents [OSTI]

    Dai, Sheng; Luo, Huimin; Baker, Gary A.

    2013-09-03T23:59:59.000Z

    Protic ionic liquids having a composition of formula (A.sup.-)(BH.sup.+) wherein A.sup.- is a conjugate base of an acid HA, and BH.sup.+ is a conjugate acid of a superbase B. In particular embodiments, BH.sup.+ is selected from phosphazenium species and guanidinium species encompassed, respectively, by the general formulas: ##STR00001## The invention is also directed to films and membranes containing these protic ionic liquids, with particular application as proton exchange membranes for fuel cells.

  12. CER4 Encodes an Alcohol-Forming Fatty Acyl-Coenzyme A Reductase Involved in Cuticular Wax Production

    E-Print Network [OSTI]

    Kunst, Ljerka

    CER4 Encodes an Alcohol-Forming Fatty Acyl-Coenzyme A Reductase Involved in Cuticular Wax surfaces of land plants. It is composed of a cutin polymer matrix and waxes. Cuticular waxes are complex and characterization of CER4, a wax bio- synthetic gene from Arabidopsis (Arabidopsis thaliana). Arabidopsis cer4

  13. A ACID RAIN Audrey Gibson

    E-Print Network [OSTI]

    Toohey, Darin W.

    acid and nitric acid. Sunlight increases the rate of most of these reactions. Electric utility plants;Gas Natural Sources Concentration Carbon dioxide CO2 Decomposition 355 ppm Nitric oxide NO Electric, 2010 #12;Gas Non-Natural Sources Concentration Nitric oxide NO Internal Combustion (cars) 0.2 ppm

  14. High octane ethers from synthesis gas-derived alcohols

    SciTech Connect (OSTI)

    Klier, K.; Herman, R.G.; Feeley, O.C.; Johansson, M.A.

    1992-07-01T23:59:59.000Z

    The objective of the proposed research is to synthesize high octane ethers, primarily methyl isobutyl ether (MIBE) and methyl tertiary butyl ether (MTBE), directly from H[sub 2]/CO/CO[sub 2] coal-derived synthesis gas via alcohol mixtures that are rich in methanol and 2-methyl-l-propanol (isobutanol). The overall scheme involves gasification of coal, purification and shifting of the synthesis gas, higher alcohol synthesis, and direct synthesis of ethers. The last stage of the synthesis involves direct coupling of synthesis gas-derived methanol and isobutanol that has been demonstrated by us to occur over superacid catalysts to yield methyl isobutyl ether (MIBE) at moderate pressures and a mixture of methanol and isobutene at low pressures. MIBE is an isomer of MTBE and a process is proposed whereby MTBE from the two alcohols is maximized and MIBE is minimized. This will be achieved by the proper choice of reaction conditions, i.e. intermediate pressures, and of inorganic acid catalysts that are stable at temperatures higher than 200[degree]C, at which the carbonium ion reaction coupling of the two alcohols to MTBE is more effective than the oxonium ion or ester reaction coupling to MIBE. Both organic and inorganic catalysts will be investigated, and the better catalysts of these classes will be subjected to long term performance studies. The long term performance studies of the combined process will extend to 1000 hr and detailed analytical data for all products will be provided.

  15. Effects of Continuous Triiodothyronine Infusion on Citric Acid Cycle in the Normal Immature Swine Heart under Extracorporeal Membrane Oxygenation in vivo

    SciTech Connect (OSTI)

    Kajimoto, Masaki; O'Kelly-Priddy, Colleen M.; Ledee, Dolena R.; Xu, Chun; Isern, Nancy G.; Olson, Aaron; Portman, Michael A.

    2014-02-15T23:59:59.000Z

    Extracorporeal membrane oxygenation (ECMO) is frequently used in infants with postoperative cardiopulmonary failure. ECMO also suppresses circulating triiodothyronine (T3) levels and modifies myocardial metabolism. We assessed the hypothesis that T3 supplementation reverses ECMO induced metabolic abnormalities in the immature heart. Twenty-two male Yorkshire pigs (age 25-38 days) with ECMO were received [2-13C]lactate, [2,4,6,8-13C]octanoate (medium chain fatty acid) and [U-13C]long-chain fatty acids as metabolic tracers either systemically (totally physiological intracoronary concentration) or directly into the coronary artery (high substrate concentration) for the last 60 minutes of each protocol. Nuclear magnetic resonance (NMR) analysis of left ventricular tissue determined the fractional contribution (Fc) of these substrates to the citric acid cycle (CAC). Fifty percent of the pigs in each group received intravenous T3 supplement (bolus at 0.6 ?g/kg and then continuous infusion at 0.2 ?g/kg/hour) during ECMO. Under both substrate loading conditions T3 significantly increased lactate-Fc with a marginal increase in octanoate-Fc. Both T3 and high substrate provision increased myocardial energy status indexed by [Phosphocreatine]/[ATP]. In conclusion, T3 supplementation promoted lactate metabolism to the CAC during ECMO suggesting that T3 releases inhibition of pyruvate dehydrogenase. Manipulation of substrate utilization by T3 may be used therapeutically during ECMO to improve resting energy state and facilitate weaning.

  16. Nucleic acids encoding human trithorax protein

    DOE Patents [OSTI]

    Evans, Glen A. (Encinitas, CA); Djabali, Malek (Marseilles, FR); Selleri, Licia (Del Mar, CA); Parry, Pauline (San Diego, CA)

    2001-01-01T23:59:59.000Z

    In accordance with the present invention, there is provided an isolated peptide having the characteristics of human trithorax protein (as well as DNA encoding same, antisense DNA derived therefrom and antagonists therefor). The invention peptide is characterized by having a DNA binding domain comprising multiple zinc fingers and at least 40% amino acid identity with respect to the DNA binding domain of Drosophila trithorax protein and at least 70% conserved sequence with respect to the DNA binding domain of Drosophila trithorax protein, and wherein said peptide is encoded by a gene located at chromosome 11 of the human genome at q23. Also provided are methods for the treatment of subject(s) suffering from immunodeficiency, developmental abnormality, inherited disease, or cancer by administering to said subject a therapeutically effective amount of one of the above-described agents (i.e., peptide, antagonist therefor, DNA encoding said peptide or antisense DNA derived therefrom). Also provided is a method for the diagnosis, in a subject, of immunodeficiency, developmental abnormality, inherited disease, or cancer associated with disruption of chromosome 11 at q23.

  17. Modeling of the formation of short-chain acids in propane flames

    E-Print Network [OSTI]

    Battin-Leclerc, Frédérique; Jaffrezo, J L; Legrand, M

    2009-01-01T23:59:59.000Z

    In order to better understand their potential formation in combustion systems, a detailed kinetic mechanism for the formation of short-chain monocarboxylic acids, formic (HCOOH), acetic (CH3COOH), propionic (C2H5COOH) and propenic (C2H3COOH)) acids, has been developed. Simulations of lean (equivalence ratios from 0.9 to 0.48) laminar premixed flames of propane stabilized at atmospheric pressure with nitrogen as diluent have been performed. It was found that amounts up to 25 ppm of acetic acid, 15 ppm of formic acid and 1 ppm of C3 acid can be formed for some positions in the flames. Simulations showed that the more abundant C3 acid formed is propenic acid. A quite acceptable agreement has been obtained with the scarce results from the literature concerning oxygenated compounds, including aldehydes (CH2O, CH3CHO) and acids. A reaction pathways analysis demonstrated that each acid is mainly derived from the aldehyde of similar structure.

  18. Metabolism of Thioctic Acid in Algae

    E-Print Network [OSTI]

    Grisebach, Hans; Fuller, R.C.; Calvin, M.

    1956-01-01T23:59:59.000Z

    METABOLISM OF THlOCTlC ACID IN ALGAE TWO-WEEK LOAN COPY ThisMETABOLISM OF THIOCTIC ACID IN ALGAE Hans Grisebach, R. , C.METABOLISM OF THIOCTIC ACID IN ALGAE Hans Grisebach, R. C.

  19. acetic acid solutions: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been...

  20. arachidonic acid activation: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been...

  1. acid inertness studies: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been...

  2. acid alleviates decreases: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been...

  3. acid activated montmorillonite: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been...

  4. acid amide hydrolase: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been studied....

  5. acid chelation phototherapeutic: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been...

  6. acid phosphatase activity: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been...

  7. acetic acid solution: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been...

  8. acid 2-benzothiazolylthiomethyl ester: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been...

  9. acetic acid operational: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been...

  10. acid phosphatase activities: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been...

  11. acid sphingomyelinase activity: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been...

  12. acids decreases fibrinolysis: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been...

  13. acid potassium glycyrrhetinate: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been...

  14. arachidonic acid activates: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been...

  15. acid decarboxylase activity: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been...

  16. acid activates nrf2: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been...

  17. acid processing activity: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been...

  18. ascorbic acid enhances: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been...

  19. acid incorporating poloxamer: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been...

  20. acid cupric chloride: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been...

  1. acids increase cardiovascular: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been...

  2. The Theory of Quaternion Matrix Derivatives

    E-Print Network [OSTI]

    Dongpo Xu; Danilo P. Mandic

    2014-10-01T23:59:59.000Z

    A systematic theory is introduced for calculating the derivatives of quaternion matrix function with respect to quaternion matrix variables. The proposed methodology is equipped with the matrix product rule and chain rule and it is able to handle both analytic and nonanalytic functions. This corrects a flaw in the existing methods, that is, the incorrect use of the traditional product rule. In the framework introduced, the derivatives of quaternion matrix functions can be calculated directly without the differential of this function. Key results are summarized in tables. Several examples show how the quaternion matrix derivatives can be used as an important tool for solving problems related to signal processing.

  3. In vivo incorporation of unnatural amino acids

    DOE Patents [OSTI]

    Schultz, Peter G. (La Jolla, CA); Wang, Lei (San Diego, CA); Anderson, John Christopher (San Diego, CA); Chin, Jason W. (Cambridge, GB); Liu, David R. (Lexington, MA); Magliery, Thomas J. (North Haven, CT); Meggers, Eric L. (Philadelphia, PA); Mehl, Ryan Aaron (Lancaster, PA); Pastrnak, Miro (San Diego, CA); Santoro, Stephen William (Cambridge, MA); Zhang, Zhiwen (San Diego, CA)

    2012-05-08T23:59:59.000Z

    The invention provides methods and compositions for in vivo incorporation of unnatural amino acids. Also provided are compositions including proteins with unnatural amino acids.

  4. Carbonic Acid Shows Promise in Geology, Biology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The Surprising Secrets of Carbonic Acid Probing the Surprising Secrets of Carbonic Acid Berkeley Lab Study Holds Implications for Geological and Biological Processes October 23,...

  5. Comprehension by Derivation Douglas R. Smith

    E-Print Network [OSTI]

    Comprehension by Derivation Douglas R. Smith Kestrel Institute 3260 Hillview Avenue Palo Alto, California 94304 USA smith@kestrel.edu Abstract We argue that to comprehend a software system is to have

  6. Estimating Derivatives of Noisy Simulations1

    E-Print Network [OSTI]

    2010-11-02T23:59:59.000Z

    derivative f (x0;p) by examining the errors. E(h) = (f(x0 + hp) ... Following [13], we scale the matrices by their diagonals and randomly select the base point x0 and ...

  7. Tax Credit for Forest Derived Biomass

    Broader source: Energy.gov [DOE]

    Forest-derived biomass includes tree tops, limbs, needles, leaves, and other woody debris leftover from activities such as timber harvesting, forest thinning, fire suppression, or forest health m...

  8. Direct synthesis of pyridine and pyrimidine derivatives

    E-Print Network [OSTI]

    Hill, Matthew D. (Matthew Dennis)

    2008-01-01T23:59:59.000Z

    I. Synthesis of Substituted Pyridine Derivatives via the Ruthenium-Catalyzed Cycloisomerization of 3-Azadienynes. The two-step conversion of various N-vinyl and N-aryl amides to the corresponding substituted pyridines and ...

  9. SCM Forcing Data Derived from NWP Analyses

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Jakob, Christian

    Forcing data, suitable for use with single column models (SCMs) and cloud resolving models (CRMs), have been derived from NWP analyses for the ARM (Atmospheric Radiation Measurement) Tropical Western Pacific (TWP) sites of Manus Island and Nauru.

  10. Modelling and Simulating of Rain Derivatives

    E-Print Network [OSTI]

    Modelling and Simulating of Rain Derivatives Master thesis Cathrin van Emmerich Supervisor, Februar 2005 Cathrin van Emmerich i #12;Table of Contents Table of Contents ii 1 Introduction 1 2

  11. Entanglement entropy in higher derivative holography

    E-Print Network [OSTI]

    Arpan Bhattacharyya; Apratim Kaviraj; Aninda Sinha

    2013-08-16T23:59:59.000Z

    We consider holographic entanglement entropy in higher derivative gravity theories. Recently Lewkowycz and Maldacena arXiv:1304.4926 have provided a method to derive the equations for the entangling surface from first principles. We use this method to compute the entangling surface in four derivative gravity. Certain interesting differences compared to the two derivative case are pointed out. For Gauss-Bonnet gravity, we show that in the regime where this method is applicable, the resulting equations coincide with proposals in the literature as well as with what follows from considerations of the stress tensor on the entangling surface. Finally we demonstrate that the area functional in Gauss-Bonnet holography arises as a counterterm needed to make the Euclidean action free of power law divergences.

  12. Controlling acid rain : policy issues

    E-Print Network [OSTI]

    Fay, James A.

    1983-01-01T23:59:59.000Z

    The policy and regulatory ramifications of U.S. acid rain control programs are examined; particularly, the alternative of a receptor-oriented strategy as constrasted to emission-oriented proposals (e.g., the Mitchell bill) ...

  13. Nitrate and Prussic Acid Poisoning

    E-Print Network [OSTI]

    Stichler, Charles; Reagor, John C.

    2001-09-05T23:59:59.000Z

    Nitrate and prussic acid poisoning in cattle are noninfectious conditions that can kill livestock. This publication explains the causes and symptoms of these conditions as well as preventive measures and sampling and testing steps....

  14. Seasonalepisodic control of acid deposition

    E-Print Network [OSTI]

    Fay, James A.

    1988-01-01T23:59:59.000Z

    This report contains the climatological, technical and economic factors for episodic and seasonal control of emissions in existing power plants. Analyzing a large data set of acid deposition for the years 1982-85, we find ...

  15. Nitrate and Prussic Acid Poisoning 

    E-Print Network [OSTI]

    Stichler, Charles; Reagor, John C.

    2001-09-05T23:59:59.000Z

    Nitrate and prussic acid poisoning in cattle are noninfectious conditions that can kill livestock. This publication explains the causes and symptoms of these conditions as well as preventive measures and sampling and testing steps....

  16. ADENOSINE TRIPHOSPHATE (ATP) AND DEOXYRIBONUCLEIC ACID (DNA) CONTENT OF MARINE MICROALGAE AND BACTERIA WITH

    E-Print Network [OSTI]

    Luther, Douglas S.

    ADENOSINE TRIPHOSPHATE (ATP) AND DEOXYRIBONUCLEIC ACID (DNA) CONTENT OF MARINE MICROALGAE the relationship between DNA and ATP content of marine bacteria and microalgae. This relationship was used. Laboratory-derived DNA:ATP ratios ranged from 8.5 to 33 (wt:wt) for cultures of marine microalgae, and from

  17. Poly(lactic acid) degradable plastics, coatings, and binders

    SciTech Connect (OSTI)

    Bonsignore, P.V.; Coleman, R.D.; Mudde, J.P.

    1992-01-01T23:59:59.000Z

    Biochemical processes to derive value from the management of high carbohydrate food wastes, such as potato starch, corn starch, and cheese whey permeate, have typically been limited to the production of either ethanol or methane. Argonne National Laboratory (ANL) believes that lactic acid presents an attractive option for an alternate fermentation end product, especially in light of lactic acids' being a viable candidate for conversion to environmentally safe poly(lactic acid) (PLA) degradable plastics, coatings, and binders. Technology is being developed at ANL to permit a more cost effective route to modified high molecular weight PLA. Preliminary data on the degradation behavior of these modified PLAs shows the retention to the inherent hydrolytic degradability of the PLA modified, however, by introduced compositional variables. A limited study was done on the hydrolytic stability of soluble oligomers of poly(L-lactic acid). Over a 34 day hold period, water-methanol solutions of Pl-LA oligomers in the 2-10 DP range retained some 75% of their original molecular weight.

  18. Poly(lactic acid) degradable plastics, coatings, and binders

    SciTech Connect (OSTI)

    Bonsignore, P.V.; Coleman, R.D.; Mudde, J.P.

    1992-05-01T23:59:59.000Z

    Biochemical processes to derive value from the management of high carbohydrate food wastes, such as potato starch, corn starch, and cheese whey permeate, have typically been limited to the production of either ethanol or methane. Argonne National Laboratory (ANL) believes that lactic acid presents an attractive option for an alternate fermentation end product, especially in light of lactic acids` being a viable candidate for conversion to environmentally safe poly(lactic acid) (PLA) degradable plastics, coatings, and binders. Technology is being developed at ANL to permit a more cost effective route to modified high molecular weight PLA. Preliminary data on the degradation behavior of these modified PLAs shows the retention to the inherent hydrolytic degradability of the PLA modified, however, by introduced compositional variables. A limited study was done on the hydrolytic stability of soluble oligomers of poly(L-lactic acid). Over a 34 day hold period, water-methanol solutions of Pl-LA oligomers in the 2-10 DP range retained some 75% of their original molecular weight.

  19. Succinic acid production by Anaerobiospirillum succiniciproducens

    E-Print Network [OSTI]

    , succinic acid has been produced commercially by chemical processes. Recently, however, fermentative of bacteria produce succinic acid as a fermentation end product,4 7 few species can produce it as the major 10 Previous studies showed that A. succiniciproducens produces succinic acid and acetic acid

  20. Human retroviruses and AIDS 1996. A compilation and analysis of nucleic acid and amino acid sequences

    SciTech Connect (OSTI)

    Myers, G.; Foley, B.; Korber, B. [eds.] [Los Alamos National Lab., NM (United States). Theoretical Div.] [eds.; Los Alamos National Lab., NM (United States). Theoretical Div.; Mellors, J.W. [ed.] [Univ. of Pittsburgh, PA (United States)] [ed.; Univ. of Pittsburgh, PA (United States); Jeang, K.T. [ed.] [National Institutes of Health, Bethesda, MD (United States). Molecular Virology Section] [ed.; National Institutes of Health, Bethesda, MD (United States). Molecular Virology Section; Wain-Hobson, S. [Pasteur Inst., Paris (France)] [ed.] [Pasteur Inst., Paris (France); ed.

    1997-04-01T23:59:59.000Z

    This compendium and the accompanying floppy diskettes are the result of an effort to compile and rapidly publish all relevant molecular data concerning the human immunodeficiency viruses (HIV) and related retroviruses. The scope of the compendium and database is best summarized by the five parts that it comprises: (1) Nuclear Acid Alignments and Sequences; (2) Amino Acid Alignments; (3) Analysis; (4) Related Sequences; and (5) Database Communications. Information within all the parts is updated throughout the year on the Web site, http://hiv-web.lanl.gov. While this publication could take the form of a review or sequence monograph, it is not so conceived. Instead, the literature from which the database is derived has simply been summarized and some elementary computational analyses have been performed upon the data. Interpretation and commentary have been avoided insofar as possible so that the reader can form his or her own judgments concerning the complex information. In addition to the general descriptions of the parts of the compendium, the user should read the individual introductions for each part.

  1. Conversion of carboxylate salts to carboxylic acids via reactive distillation

    E-Print Network [OSTI]

    Williamson, Shelly Ann

    2000-01-01T23:59:59.000Z

    biomass used in this study . . . 1-2 Approxitnate annual production and spot selling price of various carboxylic acids. 1-3 Names used for biomass feedstocks. . 1-4 2-1 Sample processing. Rinsing scheme for precipitated solids. 12 3-1 Concentration... fermentation broth derived trom various wastes. Table 1-4 describes the studies performed for each fermentation broth, as well as reagent-grade chemicals. Table 1-3. Names used for biomass feedstocks. Sample Name MSW1 MSW2 MSW3 MSW4 MSWS PF/Biol PF...

  2. Moment Methods for Exotic Volatility Derivatives

    E-Print Network [OSTI]

    Albanese, Claudio

    2007-01-01T23:59:59.000Z

    The latest generation of volatility derivatives goes beyond variance and volatility swaps and probes our ability to price realized variance and sojourn times along bridges for the underlying stock price process. In this paper, we give an operator algebraic treatment of this problem based on Dyson expansions and moment methods and discuss applications to exotic volatility derivatives. The methods are quite flexible and allow for a specification of the underlying process which is semi-parametric or even non-parametric, including state-dependent local volatility, jumps, stochastic volatility and regime switching. We find that volatility derivatives are particularly well suited to be treated with moment methods, whereby one extrapolates the distribution of the relevant path functionals on the basis of a few moments. We consider a number of exotics such as variance knockouts, conditional corridor variance swaps, gamma swaps and variance swaptions and give valuation formulas in detail.

  3. Process for the reclamation of battery acid and fluid from expended lead-acid batteries

    SciTech Connect (OSTI)

    Spitz, R.A.

    1990-11-20T23:59:59.000Z

    This patent describes a method for recycling contaminated sulfuric acid from lead acid batteries to reclaimed sulfuric acid fore reuse in the batteries by removing contaminating iron impurities. It comprises: diluting the contaminated sulfuric acid to a concentration between 150 and 230 grams per liter; filtering the sulfuric acid through a first filter means to remove solid impurities.

  4. High ethanol producing derivatives of Thermoanaerobacter ethanolicus

    DOE Patents [OSTI]

    Ljungdahl, Lars G. (Athens, GA); Carriera, Laura H. (Athens, GA)

    1983-01-01T23:59:59.000Z

    Derivatives of the newly discovered microorganism Thermoanaerobacter ethanolicus which under anaerobic and thermophilic conditions continuously ferment substrates such as starch, cellobiose, glucose, xylose and other sugars to produce recoverable amounts of ethanol solving the problem of fermentations yielding low concentrations of ethanol using the parent strain of the microorganism Thermoanaerobacter ethanolicus are disclosed. These new derivatives are ethanol tolerant up to 10% (v/v) ethanol during fermentation. The process includes the use of an aqueous fermentation medium, containing the substrate at a substrate concentration greater than 1% (w/v).

  5. High ethanol producing derivatives of Thermoanaerobacter ethanolicus

    DOE Patents [OSTI]

    Ljungdahl, L.G.; Carriera, L.H.

    1983-05-24T23:59:59.000Z

    Derivatives of the newly discovered microorganism Thermoanaerobacter ethanolicus which under anaerobic and thermophilic conditions continuously ferment substrates such as starch, cellobiose, glucose, xylose and other sugars to produce recoverable amounts of ethanol solving the problem of fermentations yielding low concentrations of ethanol using the parent strain of the microorganism Thermoanaerobacter ethanolicus are disclosed. These new derivatives are ethanol tolerant up to 10% (v/v) ethanol during fermentation. The process includes the use of an aqueous fermentation medium, containing the substrate at a substrate concentration greater than 1% (w/v).

  6. Process for removal of ammonia and acid gases from contaminated waters

    DOE Patents [OSTI]

    King, C.J.; Mackenzie, P.D.

    1982-09-03T23:59:59.000Z

    Contaminating basic gases, i.e., ammonia and acid gases, e.g., carbon dioxide, are removed from process waters or waste waters in a combined extraction and stripping process. Ammonia in the form of ammonium ion is extracted by an immiscible organic phase comprising a liquid cation exchange component, especially an organic phosphoric acid derivative, and preferably di-2-ethyl hexyl phosphoric acid, dissolved in an alkyl hydrocarbon, aryl hydrocarbon, higher alcohol, oxygenated hydrocarbon, halogenated hydrocarbon, and mixtures thereof. Concurrently, the acidic gaseous contaminants are stripped from the process or waste waters by stripping with stream, air, nitrogen, or the like. The liquid cation exchange component has the ammonia stripped therefrom by heating, and the component may be recycled to extract additional amounts of ammonia.

  7. Process for removal of ammonia and acid gases from contaminated waters

    DOE Patents [OSTI]

    King, C. Judson (Kensington, CA); MacKenzie, Patricia D. (Berkeley, CA)

    1985-01-01T23:59:59.000Z

    Contaminating basic gases, i.e., ammonia, and acid gases, e.g., carbon dioxide, are removed from process waters or waste waters in a combined extraction and stripping process. Ammonia in the form of ammonium ion is extracted by an immiscible organic phase comprising a liquid cation exchange component, especially an organic phosphoric acid derivative, and preferably di-2-ethyl hexyl phosphoric acid, dissolved in an alkyl hydrocarbon, aryl hydrocarbon, higher alcohol, oxygenated hydrocarbon, halogenated hydrocarbon, and mixtures thereof. Concurrently, the acidic gaseous contaminants are stripped from the process or waste waters by stripping with steam, air, nitrogen, or the like. The liquid cation exchange component has the ammonia stripped therefrom by heating, and the component may be recycled to extract additional amounts of ammonia.

  8. Impact of carbohydrate restriction with and without fatty acid loading on myocardial 18F-FDG uptake during PET: A randomized controlled trial

    E-Print Network [OSTI]

    Cheng, Victor Y.; Slomka, Piotr J.; Ahlen, Marie; Thomson, Louise E.; Waxman, Alan D.; Berman, Daniel S.

    2010-01-01T23:59:59.000Z

    18 F-FDG uptake during PET: A randomized controlled trialmay enhance 18 F-FDG-PET-based imaging of small, in?amedpreparations prior to 18 F-FDG-PET. Methods and Results. We

  9. Effect of HZE radiation and diets rich in fiber and n-3 poly unsaturated fatty acids (n-3 PUFA) on colon cancer in rats

    E-Print Network [OSTI]

    Glagolenko, Anna Anatolievna

    2006-08-16T23:59:59.000Z

    This study examines the carcinogenic effect of HZE radiation and protective effects of different types of diets against colon carcinogenesis in a rat model. The effect of HZE radiation on health state and colon cancer development was evaluated. HZE...

  10. Effect of HZE radiation and diets rich in fiber and n-3 poly unsaturated fatty acids (n-3 PUFA) on colon cancer in rats 

    E-Print Network [OSTI]

    Glagolenko, Anna Anatolievna

    2006-08-16T23:59:59.000Z

    This study examines the carcinogenic effect of HZE radiation and protective effects of different types of diets against colon carcinogenesis in a rat model. The effect of HZE radiation on health state and colon cancer development was evaluated. HZE...

  11. Dietary long-chain omega-3 fatty acids of marine origin: a comparison of their protective effects on coronary heart disease and breast cancers

    E-Print Network [OSTI]

    Boyer, Edmond

    (France). *Centre de Recherches Biologiques, Chemin de Montifault, 18800 Baugy (France) Corresponding 37032 TOURS Cedex (France) Tel: (+33) 2 47 36 61 30 Fax: (+33) 2 47 36 62 26 Email: Jean;90(1-3):299-325" DOI : 10.1016/j.pbiomolbio.2005.05.006 #12;2 ABSTRACT The relationship between high fish consumption

  12. The effect of different levels of forage and fish meal on the live performance and rumen volatile fatty acid concentation of heifers fed high molasses diets

    E-Print Network [OSTI]

    Estrada, Sergio

    1973-01-01T23:59:59.000Z

    in a high molasses diet is of critical economical and nutritional importance. Developing an appropriate system of feeding high levels of molasses (B5/. or more of the ration) to cattle could drastically change the horizons of cattle feeding... of molasses as the major energy source in beef cattle fattening rations. In recent years Preston and co-worl ers undertook the feeding of molasses-urea mixtures, first as a supplement to ad libitum grain feeding. Later they developed a feeding system...

  13. By studying the mechanisms of wax and oil production in plants and genetically manipulating their fatty acids chains, Ljerka Kunst hopes

    E-Print Network [OSTI]

    Karczmarek, Joanna

    By studying the mechanisms of wax and oil production in plants and genetically manipulating, locally- produced seed oils for industrial applications. All land plants produce a thin wax coating and altering the complex array of enzy- matic reactions involved in wax production is an integral part of plant

  14. The effect of wheat bran and pectin supplementation on luminal pH and short chain fatty acid production in the rat large intestine

    E-Print Network [OSTI]

    Parish, Pamela Denise

    1985-01-01T23:59:59.000Z

    , Jeanne Gazzaniga. Without Jeanne there would be no one to complain to that really understands that doing this research and writing this thesis is "THE most difficult thing in the world. " She has taught me that to accomplish anything you need a sense...

  15. Omega-3 fatty acid rich eggs as an alternative to fish in the diet: effects on human platelet aggregation and plasma lipids

    E-Print Network [OSTI]

    Mayo, Patricia

    1996-01-01T23:59:59.000Z

    aggregation, and a longer bleeding time, contributed to a lower risk of heart disease among Eskimos when compared to Danes. Eskimo platelet phospholipids (PPL) were indicative of a diet rich in marine-type omega-3 FA, specifically a low content (&I... that decreasing platelet aggregation may reduce heart disease mortality. Bang and Dyerberg (20) proposed that PPL EPA mediated interactions between platelets and vascular endothelia resulting in the production of an anti-inflanunatory, anti- constrictive...

  16. Carcass characteristics, fatty acids, stearoyl-coa desaturase gene expression and sensory evaluation of calf-fed and yearling-fed angus steers

    E-Print Network [OSTI]

    Brooks, Matthew Alan

    2009-05-15T23:59:59.000Z

    body weight, their MUFA:SFA ratio will be the same regardless of being calf-fed (CF) or yearling-fed (YF). Twenty-four Angus cattle were acquired for this study. Cattle were slaughtered at weaning at 8 mo of age (SFCF, n=4), eight steers were assigned...

  17. The effect of a controlled manipulation of maternal dietary fat intake on medium and long chain fatty acids in human breast milk in Saskatoon, Canada

    E-Print Network [OSTI]

    Nasser, Roseann; Stephen, Alison M; Goh, Yeow K; Clandinin, M Thomas

    2010-02-19T23:59:59.000Z

    Nutr Soc 2007, 66:397-404. 2. Hachey DL, Thomas MR, Emken EA, Garza C, Brown-Booth L, Adlof RO, Klein PD: Human lactation: maternal transfer to dietary triglycerides labeled with stable isotopes. J Lipid Res 1987, 28:1185-1192. 3. Emken EA, Adlof RO... . Within-feed and between-breast variation in selected components of human milk. Am J Clin Nutr 1984, 40:635-646. 20. Jensen RG: Determination of lipid content. The Lipids of Human Milk Boca Raton: CRC PressJensen RG 1989, 25-41. 21. Hall B: Uniformity...

  18. Impact of carbohydrate restriction with and without fatty acid loading on myocardial 18F-FDG uptake during PET: A randomized controlled trial

    E-Print Network [OSTI]

    Cheng, Victor Y.; Slomka, Piotr J.; Ahlen, Marie; Thomson, Louise E.; Waxman, Alan D.; Berman, Daniel S.

    2010-01-01T23:59:59.000Z

    coronary arteries with 18F-FDG PET/CT in patients with sup-acquired in a Gemini PET/CT scanner (Philips Healthcare,in a quiet room for 60 minutes. CT and PET images were then

  19. Carbide-derived carbons - From porous networks to nanotubes and...

    Office of Scientific and Technical Information (OSTI)

    Carbide-derived carbons - From porous networks to nanotubes and graphene Re-direct Destination: Carbide-derived carbons (CDCs) are a large family of carbon materials derived from...

  20. PRICING ENERGY DERIVATIVES BY LINEAR PROGRAMMING: TOLLING AGREEMENT CONTRACTS

    E-Print Network [OSTI]

    Uryasev, Stanislav

    PRICING ENERGY DERIVATIVES BY LINEAR PROGRAMMING: TOLLING AGREEMENT CONTRACTS Valeriy Ryabchenko for pricing energy derivatives known as tolling agreement contracts. The pricing problem is reduced energy derivatives. The problem of pricing such contracts falls into the class of multiple optimal

  1. Higher Derivative D-brane Couplings

    E-Print Network [OSTI]

    Guo, Guangyu

    2012-10-19T23:59:59.000Z

    supersymmetry. In the third part, we obtain the higher derivative D-brane action by using both linearized T-duality and string disc amplitude computation. We evaluate disc amplitude of one R-R field C^(p-3) and two NS-NS fields in the presence of a single Dp...

  2. Derivation of a Stochastic Neutron Transport Equation

    E-Print Network [OSTI]

    Edward J. Allen

    2010-04-14T23:59:59.000Z

    Stochastic difference equations and a stochastic partial differential equation (SPDE) are simultaneously derived for the time-dependent neutron angular density in a general three-dimensional medium where the neutron angular density is a function of position, direction, energy, and time. Special cases of the equations are given such as transport in one-dimensional plane geometry with isotropic scattering and transport in a homogeneous medium. The stochastic equations are derived from basic principles, i.e., from the changes that occur in a small time interval. Stochastic difference equations of the neutron angular density are constructed, taking into account the inherent randomness in scatters, absorptions, and source neutrons. As the time interval decreases, the stochastic difference equations lead to a system of Ito stochastic differential equations (SDEs). As the energy, direction, and position intervals decrease, an SPDE is derived for the neutron angular density. Comparisons between numerical solutions of the stochastic difference equations and independently formulated Monte Carlo calculations support the accuracy of the derivations.

  3. Deriving Security Requirements from Crosscutting Threat Descriptions

    E-Print Network [OSTI]

    Haley, Charles B.

    Deriving Security Requirements from Crosscutting Threat Descriptions Charles B. Haley, Robin C representing threats as crosscutting concerns aids in determining the effect of security requirements on the functional requirements. Assets (objects that have value in a system) are first enumerated, and then threats

  4. Wave function derivation of the JIMWLK equation

    E-Print Network [OSTI]

    Alexey V. Popov

    2008-12-16T23:59:59.000Z

    Using the stationary lightcone perturbation theory, we propose the complete and careful derivation the JIMWLK equation. We show that the rigorous treatment requires the knowledge of a boosted wave function with second order accuracy. Previous wave function approaches are incomplete and implicitly used the time ordered perturbation theory, which requires a usage of an external target field.

  5. Wind information derived from hot air

    E-Print Network [OSTI]

    Haak, Hein

    Wind information derived from hot air balloon flights for use in short term wind forecasts E Introduction/Motivation Hot air balloons as wind measuring device Setup of nested HIRLAM models Results · Three, The Nertherlands #12;Hot air balloon ·Displacement/time unit = wind speed ·Vertical resolution 30m ·Inertia (500 kg

  6. Biofuels and bio-products derived from

    E-Print Network [OSTI]

    Ginzel, Matthew

    NEED Biofuels and bio- products derived from lignocellulosic biomass (plant materials) are part improve the energy and carbon efficiencies of biofuels production from a barrel of biomass using chemical and thermal catalytic mechanisms. The Center for Direct Catalytic Conversion of Biomass to Biofuels IMPACT

  7. Background and Motivation Biomass derived syngas contains

    E-Print Network [OSTI]

    Das, Suman

    Background and Motivation · Biomass derived syngas contains: CO, H2, small hydrocarbons, H2S shown to be effective for syngas conditioning 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 0 1 2 3 Co2+(molm-2

  8. High speed point derivative microseismic detector

    DOE Patents [OSTI]

    Uhl, James Eugene (Albuquerque, NM); Warpinski, Norman Raymond (Albuquerque, NM); Whetten, Ernest Blayne (Albuquerque, NM)

    1998-01-01T23:59:59.000Z

    A high speed microseismic event detector constructed in accordance with the present invention uses a point derivative comb to quickly and accurately detect microseismic events. Compressional and shear waves impinging upon microseismic receiver stations disposed to collect waves are converted into digital data and analyzed using a point derivative comb including assurance of quiet periods prior to declaration of microseismic events. If a sufficient number of quiet periods have passed, the square of a two point derivative of the incoming digital signal is compared to a trip level threshold exceeding the determined noise level to declare a valid trial event. The squaring of the derivative emphasizes the differences between noise and signal, and the valid event is preferably declared when the trip threshold has been exceeded over a temporal comb width to realize a comb over a given time period. Once a trial event has been declared, the event is verified through a spatial comb, which applies the temporal event comb to additional stations. The detector according to the present invention quickly and accurately detects initial compressional waves indicative of a microseismic event which typically exceed the ambient cultural noise level by a small amount, and distinguishes the waves from subsequent larger amplitude shear waves.

  9. High speed point derivative microseismic detector

    DOE Patents [OSTI]

    Uhl, J.E.; Warpinski, N.R.; Whetten, E.B.

    1998-06-30T23:59:59.000Z

    A high speed microseismic event detector constructed in accordance with the present invention uses a point derivative comb to quickly and accurately detect microseismic events. Compressional and shear waves impinging upon microseismic receiver stations disposed to collect waves are converted into digital data and analyzed using a point derivative comb including assurance of quiet periods prior to declaration of microseismic events. If a sufficient number of quiet periods have passed, the square of a two point derivative of the incoming digital signal is compared to a trip level threshold exceeding the determined noise level to declare a valid trial event. The squaring of the derivative emphasizes the differences between noise and signal, and the valid event is preferably declared when the trip threshold has been exceeded over a temporal comb width to realize a comb over a given time period. Once a trial event has been declared, the event is verified through a spatial comb, which applies the temporal event comb to additional stations. The detector according to the present invention quickly and accurately detects initial compressional waves indicative of a microseismic event which typically exceed the ambient cultural noise level by a small amount, and distinguishes the waves from subsequent larger amplitude shear waves. 9 figs.

  10. Synthesis of methyl methacrylate from coal-derived syngas: Quarterly report,, October 1-December 31, 1997

    SciTech Connect (OSTI)

    NONE

    1998-09-01T23:59:59.000Z

    Research Triangle Institute (RTI), Eastman Chemical Company, and Bechtel collectively are developing a novel process for the synthesis of methyl methacrylate (MMA) from coal-derived syngas that consists of three steps of synthesis of a propionate, its condensation with formaldehyde, and esterification of resulting methacrylic acid (MAA) with methanol to produce MMA. Over the last quarter, Eastman developed two new processes which have resulted in two new invention reports. One process deals with carbonylation of benzyl ether which represents a model for coal liquefaction and the second focuses on the acceleration of carbonylation rates for propionic acid synthesis, via use of polar aprotic solvents. These two inventions are major improvements in the novel Mo-catalyzed homogeneous process for propionic acid synthesis technology, developed by Eastman. Over the last quarter, RTI completed three reaction cycles and two regeneration cycles as a part of long-term reaction regeneration cycle study on a 10% Nb{sub 2}O{sub 5}/Si0{sub 2} catalyst, for vapor phase condensation reaction of formaldehyde with propionic acid.

  11. Interaction of coal-derived synthesis gas impurities with solid...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    coal-derived synthesis gas impurities with solid oxide fuel cell metallic components. Interaction of coal-derived synthesis gas impurities with solid oxide fuel cell metallic...

  12. Phosphine oxide derivatives as hosts for blue phosphors: A joint...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    oxide derivatives as hosts for blue phosphors: A joint theoretical and experimental study of their electronic Phosphine oxide derivatives as hosts for blue phosphors: A joint...

  13. Precise attention filters for Weber contrast derived from centroid estimations

    E-Print Network [OSTI]

    Sperling, George

    Precise attention filters for Weber contrast derived from centroid estimations Department attention filters for Weber contrast derived from centroid estimations. Journal of Vision, 10(10):20, 1

  14. Agenda for the Derived Liquids to Hydrogen Distributed Reforming...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Derived Liquids to Hydrogen Distributed Reforming Working Group (BILIWG) Hydrogen Production Technical Team Research Review Agenda for the Derived Liquids to Hydrogen Distributed...

  15. BILIWG Meeting: High Pressure Steam Reforming of Bio-Derived...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    High Pressure Steam Reforming of Bio-Derived Liquids (Presentation) BILIWG Meeting: High Pressure Steam Reforming of Bio-Derived Liquids (Presentation) Presented at the 2007...

  16. amino acetal derivative: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    affects the values of the derivatives. Our constructions use randomness extractors and expander graphs. We study our derivatives in a more general setting than Arora et al. In...

  17. Progress toward Biomass and Coal-Derived Syngas Warm Cleanup...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Progress toward Biomass and Coal-Derived Syngas Warm Cleanup: Proof-of-Concept Process Demonstration of Multicontaminant Removal Progress toward Biomass and Coal-Derived Syngas...

  18. HYDROFLUORIC ACID FIRST AID INSTRUCTIONS

    E-Print Network [OSTI]

    Jalali. Bahram

    with large amounts of cool running water. Immediately washing off the acid is of primary importance. 2.Remove Immediately flush eyes for at least 15 minutes with copious cool flowing water. 2 If only one eye is affected by a glass of milk or milk of magnesia. 3 Call 911 for immediate medical assistance. REMEMBER, ALL PERSONNEL

  19. Challenges in Catalytic Manufacture of Renewable Pyrrolidinones from Fermentation Derived Succinate

    SciTech Connect (OSTI)

    White, James F.; Holladay, Johnathan E.; Zacher, Alan H.; Frye, John G.; Werpy, Todd A.

    2014-09-05T23:59:59.000Z

    Fermentation derived succinic acid ammonium salt is an ideal precursor for manufacture of renewable N-methyl pyrrolidinone (NMP) or 2-pyrrolidinone (2P) via heterogeneous catalysis. However, there are many challenges to making this a practical reality. Chief among the challenges is avoiding catalyst poisoning by fermentation by- and co-products. Battelle / Pacific Northwest National Laboratory (PNNL) have developed an economically effective technology strategy for this purpose. The technology is a combination of purely thermal processing, followed by simple catalytic hydrogenation that together avoids catalyst poisoning from fermentation impurities and provides high selectivity and yields of NMP or 2P.

  20. A derivative standard for polarimeter calibration

    SciTech Connect (OSTI)

    Mulhollan, G.; Clendenin, J.; Saez, P. [and others

    1996-10-01T23:59:59.000Z

    A long-standing problem in polarized electron physics is the lack of a traceable standard for calibrating electron spin polarimeters. While several polarimeters are absolutely calibrated to better than 2%, the typical instrument has an inherent accuracy no better than 10%. This variability among polarimeters makes it difficult to compare advances in polarized electron sources between laboratories. The authors have undertaken an effort to establish 100 nm thick molecular beam epitaxy grown GaAs(110) as a material which may be used as a derivative standard for calibrating systems possessing a solid state polarized electron source. The near-bandgap spin polarization of photoelectrons emitted from this material has been characterized for a variety of conditions and several laboratories which possess well calibrated polarimeters have measured the photoelectron polarization of cathodes cut from a common wafer. Despite instrumentation differences, the spread in the measurements is sufficiently small that this material may be used as a derivative calibration standard.