National Library of Energy BETA

Sample records for fatty acids derived

  1. Microbial engineering for the production of fatty acids and fatty...

    Office of Scientific and Technical Information (OSTI)

    Microbial engineering for the production of fatty acids and fatty acid derivatives Citation Details In-Document Search Title: Microbial engineering for the production of fatty...

  2. Technetium radiodiagnostic fatty acids derived from bisamide bisthiol ligands

    DOE Patents [OSTI]

    Jones, Alun G. (Newton Centre, MA); Lister-James, John (Wellesley, MA); Davison, Alan (Needham, MA)

    1988-05-24

    A bisamide-bisthiol ligand containing fatty acid substituted thiol useful for producing Tc-labelled radiodiagnostic imaging agents is described. The ligand forms a complex with the radionuclide .sup.99m Tc suitable for administration as a radiopharmaceutical to obtain images of the heart for diagnosis of myocardial disfunction.

  3. Microbial engineering for the production of fatty acids and fatty...

    Office of Scientific and Technical Information (OSTI)

    for the production of fatty acids and fatty acid derivatives Some aspects of this invention relate to methods useful for the conversion of a carbon source to a biofuel or...

  4. Plant fatty acid hydroxylases

    DOE Patents [OSTI]

    Somerville, Chris (Portola Valley, CA); Broun, Pierre (Burlingame, CA); van de Loo, Frank (Lexington, KY)

    2001-01-01

    This invention relates to plant fatty acyl hydroxylases. Methods to use conserved amino acid or nucleotide sequences to obtain plant fatty acyl hydroxylases are described. Also described is the use of cDNA clones encoding a plant hydroxylase to produce a family of hydroxylated fatty acids in transgenic plants. In addition, the use of genes encoding fatty acid hydroxylases or desaturases to alter the level of lipid fatty acid unsaturation in transgenic plants is described.

  5. Use of plant fatty acyl hydroxylases to produce hydroxylated fatty acids and derivatives in plants

    DOE Patents [OSTI]

    Somerville, Chris (Portola Valley, CA); van de Loo, Frank (Lexington, KY)

    2002-01-01

    The present invention relates to the identification of nucleic acid sequences and constructs, and methods related thereto, and the use of these sequences and constructs to produce genetically modified plants for the purpose of altering the composition of plant oils, waxes and related compounds.

  6. Use of plant fatty acyl hydroxylases to produce hydroxylated fatty acids and derivatives in plants

    DOE Patents [OSTI]

    Somerville, Chris (Portola Valley, CA); van de Loo, Frank (Lexington, KY)

    1998-01-01

    The present invention relates to the identification of nucleic acid sequences and constructs, and methods related thereto, and the use of these sequences and constructs to produce genetically modified plants for the purpose of altering the composition of plant oils, waxes and related compounds.

  7. Use of plant fatty acyl hydroxylases to produce hydroxylated fatty acids and derivatives in plants

    DOE Patents [OSTI]

    Somerville, Chris (Portola Valley, CA); van de Loo, Frank (Lexington, KY)

    1997-01-01

    The present invention relates to the identification of nucleic acid sequences and constructs, and methods related thereto, and the use of these sequences and constructs to produce genetically modified plants for the purpose of altering the composition of plant oils, waxes and related compounds.

  8. Use of plant fatty acyl hydroxylases to produce hydroxylated fatty acids and derivatives in plants

    DOE Patents [OSTI]

    Somerville, C.; Loo, F. van de

    1997-09-16

    The present invention relates to the identification of nucleic acid sequences and constructs, and methods related thereto, and the use of these sequences and constructs to produce genetically modified plants for the purpose of altering the composition of plant oils, waxes and related compounds. 35 figs.

  9. Use of plant fatty acyl hydroxylases to produce hydroxylated fatty acids and derivatives in plants

    DOE Patents [OSTI]

    Somerville, C.; Loo, F. van de

    1998-09-01

    The present invention relates to the identification of nucleic acid sequences and constructs, and methods related thereto, and the use of these sequences and constructs to produce genetically modified plants for the purpose of altering the composition of plant oils, waxes and related compounds. 35 figs.

  10. Fatty Acid Carcass Mapping 

    E-Print Network [OSTI]

    Turk, Stacey N.

    2010-01-14

    We hypothesized that subcutaneous (s.c.) adipose tissue would differ in monounsaturated (MUFA) and saturated fatty acid (SFA) composition among different depots throughout a beef carcass. To test this, 50 carcasses from a variety of breed types...

  11. Fatty acid-producing hosts

    DOE Patents [OSTI]

    Pfleger, Brian F; Lennen, Rebecca M

    2013-12-31

    Described are hosts for overproducing a fatty acid product such as a fatty acid. The hosts include an exogenous nucleic acid encoding a thioesterase and, optionally, an exogenous nucleic acid encoding an acetyl-CoA carboxylase, wherein an acyl-CoA synthetase in the hosts are functionally delected. The hosts prefereably include the nucleic acid encoding the thioesterase at an intermediate copy number. The hosts are preferably recominantly stable and growth-competent at 37.degree. C. Methods of producing a fatty acid product comprising culturing such hosts at 37.degree. C. are also described.

  12. Plant fatty acid hydroxylase

    DOE Patents [OSTI]

    Somerville, Chris (Portola Valley, CA); van de Loo, Frank (Lexington, KY)

    2000-01-01

    The present invention relates to the identification of nucleic acid sequences and constructs, and methods related thereto, and the use of these sequences and constructs to produce genetically modified plants for the purpose of altering the composition of plant oils, waxes and related compounds.

  13. Production of hydroxylated fatty acids in genetically modified plants

    DOE Patents [OSTI]

    Somerville, Chris; Broun, Pierre; van de Loo, Frank; Boddupalli, Sekhar S.

    2005-08-30

    This invention relates to plant fatty acyl hydroxylases. Methods to use conserved amino acid or nucleotide sequences to obtain plant fatty acyl hydroxylases are described. Also described is the use of cDNA clones encoding a plant hydroxylase to produce a family of hydroxylated fatty acids in transgenic plants. In addition, the use of genes encoding fatty acid hydroxylases or desaturases to alter the level of lipid fatty acid unsaturation in transgenic plants is described.

  14. Production of hydroxylated fatty acids in genetically modified plants

    DOE Patents [OSTI]

    Somerville, Chris (Portola Valley, CA); Broun, Pierre (Burlingame, CA); van de Loo, Frank (Weston, AU); Boddupalli, Sekhar S. (Manchester, MI)

    2011-08-23

    This invention relates to plant fatty acyl hydroxylases. Methods to use conserved amino acid or nucleotide sequences to obtain plant fatty acyl hydroxylases are described. Also described is the use of cDNA clones encoding a plant hydroxylase to produce a family of hydroxylated fatty acids in transgenic plants. In addition, the use of genes encoding fatty acid hydroxylases or desaturases to alter the level of lipid fatty acid unsaturation in transgenic plants is described.

  15. Cell Metabolism Fatty Acid Flippase Activity

    E-Print Network [OSTI]

    Chou, James

    Cell Metabolism Article Fatty Acid Flippase Activity of UCP2 Is Essential for Its Proton Transport and pathological cell growth and differentiation. Mitochondria stores energy as a pro- ton gradient across their inner membrane. Uncou- pling proteins (UCPs) can dissipate the gradient to produce heat or regulate

  16. Fatty acid synthase plays a role in cancer metabolism beyond providing fatty acids for phospholipid synthesis or sustaining elevations in glycolytic activity

    SciTech Connect (OSTI)

    Hopperton, Kathryn E.; Duncan, Robin E.; Bazinet, Richard P.; Archer, Michael C.

    2014-01-15

    Fatty acid synthase is over-expressed in many cancers and its activity is required for cancer cell survival, but the role of endogenously synthesized fatty acids in cancer is unknown. It has been suggested that endogenous fatty acid synthesis is either needed to support the growth of rapidly dividing cells, or to maintain elevated glycolysis (the Warburg effect) that is characteristic of cancer cells. Here, we investigate both hypotheses. First, we compared utilization of fatty acids synthesized endogenously from {sup 14}C-labeled acetate to those supplied exogenously as {sup 14}C-labeled palmitate in the culture medium in human breast cancer (MCF-7 and MDA-MB-231) and untransformed breast epithelial cells (MCF-10A). We found that cancer cells do not produce fatty acids that are different from those derived from exogenous palmitate, that these fatty acids are esterified to the same lipid and phospholipid classes in the same proportions, and that their distribution within neutral lipids is not different from untransformed cells. These results suggest that endogenously synthesized fatty acids do not fulfill a specific function in cancer cells. Furthermore, we observed that cancer cells excrete endogenously synthesized fatty acids, suggesting that they are produced in excess of requirements. We next investigated whether lipogenic activity is involved in the maintenance of high glycolytic activity by culturing both cancer and non-transformed cells under anoxic conditions. Although anoxia increased glycolysis 2–3 fold, we observed no concomitant increase in lipogenesis. Our results indicate that breast cancer cells do not have a specific qualitative or quantitative requirement for endogenously synthesized fatty acids and that increased de novo lipogenesis is not required to sustain elevations in glycolytic activity induced by anoxia in these cells. - Highlights: • Fatty acid synthase (FASN) is over-expressed in cancer but its function is unknown. • We compare utilization of fatty acids produced by FASN to those derived exogenously. • Cancer cells do not have a specific requirement for fatty acids produced by FASN. • Fatty acids produced by FASN are in excess of cell requirements and are excreted. • Increased FASN activity is not required to sustain elevations in glycolysis.

  17. Production of hydroxylated fatty acids in genetically modified plants

    DOE Patents [OSTI]

    Somerville, Chris (Portola Valley, CA); Broun, Pierre (Burlingame, CA); van de Loo, Frank (Lexington, KY)

    2001-01-01

    This invention relates to plant fatty acyl hydroxylases. Methods to use conserved amino acid or nucleotide sequences to obtain plant fatty acyl hydroxylases are described. Also described is the use of cDNA clones encoding a plant hydroxylase to produce a family of hydroxylated fatty acids in transgenic plants.

  18. A First Look at Yeast Fatty Acid Synthase

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    synthesis is carried out by fatty acid sythase (FAS), which catalyzes cycles of multistep chemical reactions that are essentially the same in all organisms. FAS uses one...

  19. A First Look at Yeast Fatty Acid Synthase

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Synthase Print Fatty acids are the major constituents of eukaryotic and bacterial cellular membranes. They are used for functionally important post-translational protein...

  20. A First Look at Yeast Fatty Acid Synthase

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    2007 00:00 Fatty acids are the major constituents of eukaryotic and bacterial cellular membranes. They are used for functionally important post-translational protein...

  1. Stabilized epoxygenated fatty acids regulate inflammation, pain, angiogenesis and cancer

    E-Print Network [OSTI]

    Hammock, Bruce D.

    Review Stabilized epoxygenated fatty acids regulate inflammation, pain, angiogenesis and cancer Cancer Center, University of California, Davis, CA 95616, United States b Department of Food Science

  2. Glucose oxidation in heart-type fatty acid binding protein null mice 

    E-Print Network [OSTI]

    Adhikari, Sean

    2006-10-30

    Heart-type fatty acid binding protein (H-FABP) is a major fatty acid binding factor in skeletal muscles. Genetic lack of H-FABP severely impairs the esterification and oxidation of exogenous fatty acids in soleus muscles ...

  3. An insect assay for essential fatty acids 

    E-Print Network [OSTI]

    Kerur, Dundappa Ramachandrappa

    1956-01-01

    Ltc sn6 arachl@ontc acl6s 'es members sf' the essential fatty acid group. naut; defzolencp spmy&ms in the rat, associated cath e. fat &red Met, axes s) retardee@ growth, 'b) increased z YAM a%one o ' SMce She orz. g~l recognlM;on of zznolekc...'QPGP-, ' I T~ OP QQFg~V25 P8j. , X%4&4?C 4IQ? o e''e t ~ e jt 0 e s'o X o, s t tp ~ s o o o p e o * |p 0 o ~ t v o t s o ~ ~ o o o s", I" 'a "- "" K'1844X PCS, BCVlCV p e t's ?'o s e e tt p t e t p t a ~ t' o' o s o 's ~ s' 't o t' ~, ~ o'o a' A~t8ng58...

  4. Carbon pathways to zooplankton: insights from the combined use of stable isotope and fatty acid biomarkers

    E-Print Network [OSTI]

    Mazumder, Asit

    Carbon pathways to zooplankton: insights from the combined use of stable isotope and fatty acid quantified the relative contribution of terrestrial- and phytoplankton-derived carbon sources to zooplankton secondary production in lakes. However, few investigated the pathways along which allochthonous

  5. Synergistic interaction between oxides of copper and iron for production of fatty alcohols from fatty acids

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Kandel, Kapil; Chaudhary, Umesh; Nelson, Nicholas C.; Slowing, Igor I.

    2015-10-08

    In this study, the selective hydrogenation of fatty acids to fatty alcohols can be achieved under moderate conditions (180 °C, 30 bar H2) by simultaneously supporting copper and iron oxides on mesoporous silica nanoparticles. The activity of the cosupported oxides is significantly higher than that of each supported metal oxide and of a physical mixture of both individually supported metal oxides. A strong interaction between both metal oxides is evident from dispersion, XRD, TPR, and acetic acid TPD measurements, which is likely responsible for the synergistic behavior of the catalyst. Copper oxide is reduced in situ to its metallic formmore »and thereby activates hydrogen.« less

  6. Mutant fatty acid desaturase and methods for directed mutagenesis

    DOE Patents [OSTI]

    Shanklin, John (Shoreham, NY); Whittle, Edward J. (Greenport, NY)

    2008-01-29

    The present invention relates to methods for producing fatty acid desaturase mutants having a substantially increased activity towards substrates with fewer than 18 carbon atom chains relative to an unmutagenized precursor desaturase having an 18 carbon chain length specificity, the sequences encoding the desaturases and to the desaturases that are produced by the methods. The present invention further relates to a method for altering a function of a protein, including a fatty acid desaturase, through directed mutagenesis involving identifying candidate amino acid residues, producing a library of mutants of the protein by simultaneously randomizing all amino acid candidates, and selecting for mutants which exhibit the desired alteration of function. Candidate amino acids are identified by a combination of methods. Enzymatic, binding, structural and other functions of proteins can be altered by the method.

  7. Radiolabeled dimethyl branched long chain fatty acid for heart imaging

    DOE Patents [OSTI]

    Knapp, Jr., Furn F. (Oak Ridge, TN); Goodman, Mark M. (Knoxville, TN); Kirsch, Gilbert (Woippy, FR)

    1988-08-16

    A radiolabeled long chain fatty acid for heart imaging that has dimethyl branching at one of the carbons of the chain which inhibits the extent to which oxidation can occur. The closer to the carboxyl the branching is positioned, the more limited the oxidation, thereby resulting in prolonged retention of the radiolabeled compound in the heart.

  8. Fatty Acid Composition of Fish Oils UNITED STATES DEPART MENT OF THE INTERIOR

    E-Print Network [OSTI]

    Fatty Acid Composition of Fish Oils UNITED STATES DEPART MENT OF THE INTERIOR FISH AND WILDLIFE INTRODUCTION I . of the chemical nature of fish-oil fatty acids and their dis- ~ arine life is important of the distribution of fatty acids i~ also i~por~ant in _rstand the physical and chemical properties of fIsh oils

  9. ANTIBODY PURIFICATION USING CAPRYLIC ACID In mildly acidic conditions, the addition of short-chain fatty acids such as caprylic

    E-Print Network [OSTI]

    Mecham, Robert

    ANTIBODY PURIFICATION USING CAPRYLIC ACID In mildly acidic conditions, the addition of short-chain fatty acids such as caprylic acid to serum will precipitate most serum proteins with the exception or ammonium sulfate precipitation, caprylic acid will yield a relatively pure antibody preparation. 1. Measure

  10. Individual Variation in Lipidomic Profiles of Healthy Subjects in Response to Omega-3 Fatty Acids

    E-Print Network [OSTI]

    Hammock, Bruce D.

    Individual Variation in Lipidomic Profiles of Healthy Subjects in Response to Omega-3 Fatty Acids-variately in 12 healthy subjects pre vs. post six weeks of 3 fatty acids (1.9 g/d eicosapentaenoic acid [EPA significantly different pre vs. post treatment across all subjects (p=0.00007 and p=0.00002 respectively

  11. Isolation and identification of fatty acid amides from Shengli coal

    SciTech Connect (OSTI)

    Ming-Jie Ding; Zhi-Min Zong; Ying Zong; Xiao-Dong Ou-Yang; Yao-Guo Huang; Lei Zhou; Feng Wang; Jiang-Pei Cao; Xian-Yong Wei

    2008-07-15

    Shengli coal, a Chinese brown coal, was extracted with carbon disulfide and the extract was gradiently eluted with n-hexane and ethyl acetate (EA)/n-hexane mixed solvents with different concentrations of EA in a silica gel-filled column. A series of fatty acid amides, including fourteen alkanamides (C{sub 15}-C{sub 28}) and three alkenamides (C{sub 18} and C{sub 22}), were isolated from the coal by this method and analyzed with a gas chromatography/mass spectrometry. 26 refs., 2 figs., 2 tabs.

  12. Impact of Omega-3 Polyunsaturated Fatty Acids on Bone Adaptations to Simulated Resistance Training 

    E-Print Network [OSTI]

    Camp, Kaleigh Ann

    2013-04-30

    Young and ovariectomized animals eating diets rich in omega-3 polyunsaturated fatty acids (n-3 PUFAs) exhibit enhanced bone formation and decrease bone loss, respectively. Eicosapentaenoic acid, an n-3 PUFA found in fish ...

  13. Heart- and liver-type fatty acid binding proteins in lipid and glucose metabolism 

    E-Print Network [OSTI]

    Erol, Erdal

    2004-11-15

    Heart-type Fatty Acid-Binding Protein (H-FABP) is required for high rates of skeletal muscle long chain fatty acid (LCFA) oxidation and esterification. Here we assessed whether H-FABP affects soleus muscle glucose uptake when measured in vitro...

  14. Fatty Acid Chemistry at the Oil-Water Interface: Self-Propelled Oil Droplets

    E-Print Network [OSTI]

    Ikegami, Takashi

    Fatty Acid Chemistry at the Oil-Water Interface: Self-Propelled Oil Droplets Martin M. Hanczyc transitions. Here we have explored the possibility that fatty acid systems also demonstrate movement. An oil solution. The oil droplets showed autonomous, sustained movement through the aqueous media. Internal

  15. Journal of Chromatography A, 807 (1998) 111119 Microemulsion electrokinetic chromatography of fatty acids as

    E-Print Network [OSTI]

    Miksik, Ivan

    1998-01-01

    /w) and n-butanol (6.55%, w/w), pH 10.2. Separation by microemulsion electrokinetic chromatography of fatty acids as phenacyl esters *´ Ivan Miksik , Zdenek Deyl ´ ´Institute of Physiology, Academy of saturated fatty acids containing even number of carbon atoms was successfully separated as phenacyl esters

  16. PHOSPHOLIPID AND TRIACYLGLYCEROL FATTY ACIDS IN BLOOD AND BREAST TISSUE OBTAINED FROM WOMEN AT INCREASED RISK OF BREAST CANCER

    E-Print Network [OSTI]

    Li, Shengqi

    2010-06-08

    The purpose of this study was to determine the fatty acids composition in breast tissue of women with increased risk of breast cancer, and if the fatty acid composition in both PL and TAG in blood, both plasma and RBC, correlated to the fatty acid...

  17. Influence of ethanol on fatty acid composition of phospholipids in cultured neurons

    SciTech Connect (OSTI)

    Morrisson, M.; Wilce, P.A.; Shanley, B.C.

    1984-07-31

    Animals chronically exposed to ethanol show changes in neural membrane lipids which may underlie the development of tolerance and physical dependence. The object of this study was to investigate changes in the fatty acid composition of neuronal phospholipids cultured in the presence of ethanol (55 or 110 mM) for periods up to 7 days. Decreases were observed in the percentage of individual and total saturated fatty acids, while the double bond index: total saturated fatty acid ratio, increased. These changes do not support the hypothesis that neural membrane lipid composition changes to counteract the fluidizing action of ethanol. 13 references, 2 tables.

  18. Effect of dietary cysteine, methionine, and sterculic acid on fatty acid distribution in rat adipose tissue 

    E-Print Network [OSTI]

    Brotze, Mary Frances

    1968-01-01

    . Statistical Anal sis The data were treated according to the analysis of variance for data with a single criterion of classifica- tion(24). Each of the ratios for the triglyceride frac- tion were analyzed as: Source oi Variation De rees of Freedom Total... ACIDS IN ADIPOSE TISSUE OF THE RAT B. Free Fatty Acid Fraction Group No. Sterculia f~oa ao 1 Methionine level in diet Cysteine level in diet 16/16:1 18/18:1 18/18:2 18:1/18:2 III IV VI VII VIII 0. 2 0. 2 0. 2 0. 2 low low high...

  19. Crystallization and preliminary X-ray crystallographic studies of fatty acid-CoA racemase from Mycobacterium tuberculosis H37Rv

    SciTech Connect (OSTI)

    Rhee, Kyung-Hee; Lee, Ki Seog; Priyadarshi, Amit; Kim, Eunice Eunkyung; Hwang, Kwang Yeon

    2005-11-01

    Fatty acid-CoA racemase from M. tuberculosis H37Rv has been overexpressed, purified and crystallized. Diffraction data have been collected to beyond 2.7 Å resolution using a synchrotron-radiation source. Fatty acid-CoA racemase plays an important role in the ?-oxidation of branched-chain fatty acids and fatty-acid derivatives as it catalyzes the conversion of several (2R)-branched-chain fatty acid-CoAs to their (2S)-stereoisomers. Fatty acid-CoA racemase from Mycobacterium tuberculosis H37Rv has been purified to homogeneity and crystallized by the hanging-drop vapour-diffusion method with polyethylene glycol 4000 as precipitant. The crystals belong to the trigonal space group P3{sub 1} or P3{sub 2}, with unit-cell parameters a = b = 109.56, c = 147.97 Å. The asymmetric unit contains six monomers, corresponding to a V{sub M} value of 2.15 Å{sup 3} Da{sup ?1}. A complete native data set has been collected at 2.7 Å resolution using a synchrotron-radiation source.

  20. Induction of acid and oxidative reistance in Escherichia cole 0157:H7 by exposure to short-chain fatty acids 

    E-Print Network [OSTI]

    Arnold, Carrie Nell

    2013-02-22

    Short-chain fatty acids (SCFAs) are commonly used as food preservatives to prevent microbial contamination of meat carcasses. However, the food-borne pathogen, E. coli O157:H7, is more resistant than other E. coli strains ...

  1. Exploring volatile fatty acids (VFAs) as a novel substrate for microbial oil production

    E-Print Network [OSTI]

    Chakraborty, Sagar, Ph. D. Massachusetts Institute of Technology

    2015-01-01

    Cost effective production of biofuels depends critically on feedstock cost and availability. As such, volatile fatty acids (VFAs) can play an important role in advancing sustainable biofuel production since they can be ...

  2. Imflammatory response in horses fed diets containing omega-6 and omega-3 fatty acids 

    E-Print Network [OSTI]

    Wilson, Kristopher Ray

    2003-01-01

    This study was conducted to characterize the inflammatory response of horses to chronic and acute exercise as influenced by dietary fat supplementation. Different ratios of omega 6 vs. omega 3 fatty acid supplementation ...

  3. Fatty acid composition and distribution in the blubber of bottlenose dolphins (Tursiops truncatus) 

    E-Print Network [OSTI]

    Samuel, Asha Melina

    2000-01-01

    . Lipids were extracted from each sample, and fatty acid methyl esters analyzed using a gas-liquid chromatograph connected to a computerized integration system. Statistical analysis consisted of multivariate techniques, including classification...

  4. Modulation of purified murine T cell subset activation by dietary n-3 Polyunsaturated fatty acids 

    E-Print Network [OSTI]

    Arrington, Jennifer L

    2000-01-01

    n-3 Polyunsaturated fatty acids (PUFA) have been shown to reduce inflammation in diseases such as rheumatoid arthritis. The present studies sought to elucidate mechanisms whereby n-3 PUFAs downregulate T cell proliferation and affect CD4 and CD8...

  5. Conversion of essential fatty acids by Delta 6 desaturase in dog liver microsomes 

    E-Print Network [OSTI]

    Dunbar, Brent Lee

    2001-01-01

    substrates. Following incubation, lipids were extracted, saponified and phenacylated. The resulting fatty acid phenacyl esters (FAPES) were separated by HPLC. Radioactivity was measured with a liquid scintillation counter, converted to enzymatic activity...

  6. Effects of dietary fiber and carcinogen on fatty acid binding protein expression in exfoliated colonocytes 

    E-Print Network [OSTI]

    Clark, Amy Eunice

    1997-01-01

    EFFECTS OF DIETARY FIBER AND CARCINOGEN ON FATTY ACID BINDING PROTEIN EXPRESSION IN EXFOLIATED COLONOCYTES A Thesis by AMY EUNICE CLARK Submitted to the Office of Gmduate Studies of Texas A&M University in partial fulfillment... of the requireinents for the degree of MASTER OF SCIENCE August 1997 Major Subject: Nutriuon EFFECTS OF DIETARY FIBER AND CARCINOGEN ON FATTY ACID BINDING PROTEIN EXPRESSION IN EXFOLlATED COLONOCYTES by AMY EUNICE CLARK Submitted to Texas ARM University...

  7. Modifying yolk fatty acid composition to improve the health quality of shell eggs 

    E-Print Network [OSTI]

    Van Elswyk, Mary Elizabeth

    1990-01-01

    MODIFYING YOLK FATTY ACID COMPOSITION TO IMPROVE THE HEALTH QUALITY OF SHELL EGGS A Thesis by MARY ELIZABETH VAN ELSWYK Submitted to the Office of Graduate Studies of Texas A&M University in partial fulfillment of the requirements... for the degree of MASTER OF SCIENCE August 1990 Major Subject: Nutrition MODIFYING YOLK FATTY ACID COMPOSITION TO IMPROVE THE HEALTH QUALITY OF SHELL EGGS A Thesis by MARY ELIZABETH VAN ELSWYK Approved as to style and content by: Pamela S. Har s (Chair...

  8. Influence of dietary fat on the fatty acid composition of egg yolks and reproductive performance in the turkey 

    E-Print Network [OSTI]

    Cornett, Bonita Manis

    1966-01-01

    of Soybean Oil and Neatsfoot Oil 36 Table 4 - Patty Acid Composition of Diets 37 Table 5 - Yolk Fatty Acids at One and Three Months of Dietary Treatment (Polar Praction) 38 Table 6 - Yolk Fatty Acids at One and Three Months of Dietary Treatment (Neutral... fatty acids. The following study was undertaken to evaluate (1) the effects of feeding turkey hens on a fat- free diet or diets containing un- saturated oils, on the subsequent fatty acid composition in the yolk, and (2) the effects of the various...

  9. Immunosuppressive dietary n-3 polyunsaturated fatty acids differentially modulate costimulatory regulation of murine CD4+ T-cell function 

    E-Print Network [OSTI]

    Ly, Lan H.

    2005-02-17

    Consumption of fish oils (FO) enriched with the n-3 polyunsaturated fatty acids (PUFA), docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), is beneficial to a variety of inflammatory disorders due, in part, to the alteration of membrane...

  10. A study of the distribution of fatty acids in the system: cottonseed oil-oleic acid-isopropanol-water 

    E-Print Network [OSTI]

    Lamb, Frank E

    1948-01-01

    solutions. Density, relat1ve volume, percent non-vole tile, and fatty ac1d dis- tribution were determined for e . ch phase for various isopropanol - water r st1os over a considerable range of fatty acid concentrations. Counter-current liquid.... The unsaturated. fatty ac1ds are believed (6) to be destructive to the vitamin A snd D content of the oil due to the formation of oxidation products when exposed to atmospheric conditions. For improving the stability and quality of the oil, it 1s desirable...

  11. Microbial engineering for the production of fatty acids and fatty acid derivatives

    DOE Patents [OSTI]

    Stephanopoulos, Gregory; Abidi, Syed Hussain Imam

    2014-07-01

    Some aspects of this invention relate to methods useful for the conversion of a carbon source to a biofuel or biofuel precursor using engineered microbes. Some aspects of this invention relate to the discovery of a key regulator of lipid metabolism in microbes. Some aspects of this invention relate to engineered microbes for biofuel or biofuel precursor production.

  12. Human platelet aggregation and phospholipid fatty acid composition during omega-3 fatty acid enriched egg consumption: influence of nutrient intake and omega-3 fatty acid source 

    E-Print Network [OSTI]

    Hatch, Sandra D

    1997-01-01

    , normolipidemic individuals (n=30) consumed four typical, linolenic acid-rich (LNA; 18:3n-3), or docosahexaenoic acid-rich (DHA; 22:6n-3) eggs weekly for alternating six-week periods, separated by four-week washouts, in a completely randomized design. According...

  13. Fermentation of pectin and cellulose to short chain fatty acids: a comparative study with humans, baboons, pigs, and rats 

    E-Print Network [OSTI]

    Villalba, Leonilde Nonita

    1989-01-01

    Committee: Dr. Joanne R. Lupton The short chain fatty acid (SCFA) production from the fermentation of dietary fiber may have important physiological effects on human health and disease, indicating the importance of selecting the appropriate animal model... DETERMINATION OF pH DETERMINATION OF PERCENT RECOVERY OF FIBERS 16 18 20 20 CHAPTER PAGE SHORT CHAIN FATTY ACID ANALYSIS 23 STATISTICAL ANALYSIS 24 IV. RESULTS 25 INTERSPECIES DIFFERENCE OF SHORT CHAIN FATTY PRODUCTION PROFILE OF SCFA PRODUCTION...

  14. Omega 3 fatty acids increase spontaneous release of cytosolic components from tumor cells

    SciTech Connect (OSTI)

    Jenski, L.J.; Sturdevant, L.K.; Ehringer, W.D.; Stillwell, W. (Department of Biology, Indiana University-Purdue University, Indianapolis (United States))

    1991-05-01

    Mice fed menhaden (fish) oil or coconut oil-rich diets were inoculated intraperitoneally with a rapidly growing leukemia, T27A. After one week, the tumor cells were harvested, and 51Cr was used to label intracellular molecules. Spontaneous release of 51Cr was used as a measure of plasma membrane permeability. Compared to cells from mice fed coconut oil (rich in saturated fatty acids), tumor cells from mice fed menhaden oil (rich in long chain polyunsaturated omega 3 fatty acids) showed an increased level of spontaneous 51Cr release, which was exacerbated by increased temperature and reduced by extracellular protein. At physiological salt concentrations, the released 51Cr was detected in particles of approximately 2700 daltons. Enhanced permeability correlated with the incorporation of dietary (fish oil) omega 3 polyunsaturated fatty acids docosahexaenoic and eicosapentaenoic acid into the tumor cells. The results demonstrate that omega 3 fatty acids are incorporated into cellular constituents of tumor cells and change properties associated with the plasma membrane. This result suggests that dietary manipulation may be used to enhance tumor cell permeability and contribute to tumor eradication.

  15. Novel Biosynthetic Pathway for Production of Fatty Acid Derived Molecules -

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration wouldMass map shinesSolarNew scholarshipThreeFebruaryMuseumEffect901 Federal7 AEnergy Innovation

  16. A First Look at Yeast Fatty Acid Synthase

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach HomeA Better Anode Design to Improve Lithium-Ion Batteries Print Lithium-ionA First Look at Yeast Fatty

  17. A First Look at Yeast Fatty Acid Synthase

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach HomeA Better Anode Design to Improve Lithium-Ion Batteries Print Lithium-ionA First Look at Yeast FattyA

  18. A First Look at Yeast Fatty Acid Synthase

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach HomeA Better Anode Design to Improve Lithium-Ion Batteries Print Lithium-ionA First Look at Yeast FattyAA

  19. Prediction of liquid-liquid equilibrium for systems of vegetable oils, fatty acids, and ethanol

    SciTech Connect (OSTI)

    Batista, E.; Monnerat, S.; Stragevitch, L.; Pina, C.G.; Goncalves, C.B.; Meirelles, A.J.A.

    1999-12-01

    Group interaction parameters for the UNIFAC and ASOG models were specially adjusted for predicting liquid-liquid equilibrium (LLE) for systems of vegetable oils, fatty acids, and ethanol at temperatures ranging from 20 to 45 C. Experimental liquid-liquid equilibrium data for systems of triolein, oleic acid, and ethanol and of triolein, stearic acid, and ethanol were measured and utilized in the adjustment. The average percent deviation between experimental and calculated compositions was 0.79% and 0.52% for the UNIFAC and ASOG models, respectively. The prediction of liquid-liquid equilibrium for systems of vegetable oils, fatty acids, and ethanol was quite successful, with an average deviation of 1.31% and 1.32% for the UNIFAC and ASOG models, respectively.

  20. Author's personal copy Culture performance and tissue fatty acid compositions of yellow perch

    E-Print Network [OSTI]

    perch (Perca flavescens) Vegetable oils Growth performance Fatty acids The partial or total replacement of fish oil with soybean oil or flax oil in yellow perch Perca flavescens rearing performance and tissue% fish oil, which was used as a control; 2) FO:FXO, a 1:1 mixture of fish oil and flaxseed oil; 3) FXO

  1. ADS genes for reducing saturated fatty acid levels in seed oils

    DOE Patents [OSTI]

    Heilmann, Ingo H. (Bay Shore, NY); Shanklin, John (Shoreham, NY)

    2010-02-02

    The present invention relates to enzymes involved in lipid metabolism. In particular, the present invention provides coding sequences for Arabidopsis Desaturases (ADS), the encoded ADS polypeptides, and methods for using the sequences and encoded polypeptides, where such methods include decreasing and increasing saturated fatty acid content in plant seed oils.

  2. ADS genes for reducing saturated fatty acid levels in seed oils

    DOE Patents [OSTI]

    Heilmann, Ingo H; Shanklin, John

    2014-03-18

    The present invention relates to enzymes involved in lipid metabolism. In particular, the present invention provides coding sequences for Arabidopsis Desaturases (ADS), the encoded ADS polypeptides, and methods for using the sequences and encoded polypeptides, where such methods include decreasing and increasing saturated fatty acid content in plant seed oils.

  3. Vapor Pressures and Vaporization, Sublimation, and Fusion Enthalpies of Some Fatty Acids

    E-Print Network [OSTI]

    Chickos, James S.

    Vapor Pressures and Vaporization, Sublimation, and Fusion Enthalpies of Some Fatty Acids Joe A vapor pressures of both the subcooled liquid and solid state for those materials that are solids at T/K = 298.15. Equations for the prediction of vapor pressure from T/K = 298.15 to the boiling temperature

  4. Stable carbon isotopic compositions of bacterial fatty acids in a seagrass dominated system 

    E-Print Network [OSTI]

    Jones, Walter Brian

    2001-01-01

    as a proxy of organic carbon sources to the sediment. T. testudinum above ground tissues averaged -11.8±0.3[0/00] and benthic microalgae, as represented by the fatty acid 20:5[]3, averaged -20.5±0.6[0/00]. The TO¹³C from all habitats and depths were...

  5. Dengue virus nonstructural protein 3 redistributes fatty acid synthase to sites of viral replication and

    E-Print Network [OSTI]

    | replication complex Dengue virus (DENV) is the causative agent of dengue fever, dengue hemorrhagic feverDengue virus nonstructural protein 3 redistributes fatty acid synthase to sites of viral August 27, 2010 (received for review July 23, 2010) Dengue virus (DENV) modifies cellular membranes

  6. Investigating fatty acid biosynthesis within the algal chloroplast using Chlamydomonas reinhardtii as a model

    E-Print Network [OSTI]

    Blatti, Jillian L.

    2012-01-01

    18:1-ACP and FatB TEs siphon a range of saturated acyl-suggesting that the CrTE can siphon a range of acyl-ACPthat the CrTE is able to siphon 16:0- ACPs. Fatty acid

  7. Comparison of fatty acid profiles of spawning and non-spawning Pacific herring, Clupea harengus pallasi

    E-Print Network [OSTI]

    of the Pacific rim from Korea and Baja Mexico in the South to the Arctic in the North (Haegele and SchweigertComparison of fatty acid profiles of spawning and non-spawning Pacific herring, Clupea harengus from liver, intestine, roe, milt and flesh of spawning and non-spawning Pacific herring (Clupea

  8. Essential versus potentially toxic dietary substances: A seasonal comparison of essential fatty acids and methyl mercury

    E-Print Network [OSTI]

    Mazumder, Asit

    to storage lipids that represent the primary source of metabolic energy in zooplankton during periods of low supply of essential fatty acids, while MeHg bioaccumulates with zooplankton size. Abstract We significantly with increasing plankton size and were independent of plankton taxonomy. However, total EFA

  9. Viscosity of plant oils as a function of temperature, fatty acid chain length, and unsaturation 

    E-Print Network [OSTI]

    Neo, Tong Heng

    1988-01-01

    VISCOS1TY OF PLANT OILS AS A FUNCTION OF TEMPERATURE, FATIY ACID CHAIN LENGTH, AND UNSATURATION A Thesis by TONG HENG NEO Submitted to the Office of Graduate Studies of Texas A&M University in partial fulfillment of the requirements... for the degree of MASTER OF SCIENCE December 1988 Major Subject: Agricultural Engineering VISCOSITY OF PLANT OILS AS A FUNCTION OF TEMPERATURE, FATTY ACID CHAIN LENGTH, AND UNSATURATION A Thesis by TONG HENG NEO Approved as to style and content by...

  10. Genome-wide analysis of the omega-3 fatty acid desaturase gene family in Gossypium

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Yurchenko, Olga P.; Park, Sunjung; Ilut, Daniel C.; Inmon, Jay J.; Millhollon, Jon C.; Liechty, Zach; Page, Justin T.; Jenks, Matthew A.; Chapman, Kent D.; Udall, Joshua A.; et al

    2014-11-18

    The majority of commercial cotton varieties planted worldwide are derived from Gossypium hirsutum, which is a naturally occurring allotetraploid produced by interspecific hybridization of A- and D-genome diploid progenitor species. While most cotton species are adapted to warm, semi-arid tropical and subtropical regions, and thus perform well in these geographical areas, cotton seedlings are sensitive to cold temperature, which can significantly reduce crop yields. One of the common biochemical responses of plants to cold temperatures is an increase in omega-3 fatty acids, which protects cellular function by maintaining membrane integrity. The purpose of our study was to identify and characterizemore »the omega-3 fatty acid desaturase (FAD) gene family in G. hirsutum, with an emphasis on identifying omega-3 FADs involved in cold temperature adaptation. Results: Eleven omega-3 FAD genes were identified in G. hirsutum, and characterization of the gene family in extant A and D diploid species (G. herbaceum and G. raimondii, respectively) allowed for unambiguous genome assignment of all homoeologs in tetraploid G. hirsutum. The omega-3 FAD family of cotton includes five distinct genes, two of which encode endoplasmic reticulum-type enzymes (FAD3-1 and FAD3-2) and three that encode chloroplast-type enzymes (FAD7/8-1, FAD7/8-2, and FAD7/8-3). The FAD3-2 gene was duplicated in the A genome progenitor species after the evolutionary split from the D progenitor, but before the interspecific hybridization event that gave rise to modern tetraploid cotton. RNA-seq analysis revealed conserved, gene-specific expression patterns in various organs and cell types and semi-quantitative RT-PCR further revealed that FAD7/8-1 was specifically induced during cold temperature treatment of G. hirsutum seedlings. Conclusions: The omega-3 FAD gene family in cotton was characterized at the genome-wide level in three species, showing relatively ancient establishment of the gene family prior to the split of A and D diploid progenitor species. The FAD genes are differentially expressed in various organs and cell types, including fiber, and expression of the FAD7/8-1 gene was induced by cold temperature. These data define the genetic and functional genomic properties of this important gene family in cotton and provide a foundation for future efforts to improve cotton abiotic stress tolerance through molecular breeding approaches.« less

  11. Fatty Acid Biosynthesis Caught in the Act | Stanford Synchrotron...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Acid Biosynthesis", Nature 505, 427 (2014), DOI: 10.1038nature12810. Contacts: Shiou-Chuan Tsai, University of California-Irvine Michael Burkart, University of California-San...

  12. Guiding optimal biofuels : a comparative analysis of the biochemical production of ethanol and fatty acid ethyl esters from switchgrass.

    SciTech Connect (OSTI)

    Paap, Scott M.; West, Todd H.; Manley, Dawn Kataoka; Dibble, Dean C.; Simmons, Blake Alexander; Steen, Eric J.; Beller, Harry R.; Keasling, Jay D.; Chang, Shiyan

    2013-01-01

    In the current study, processes to produce either ethanol or a representative fatty acid ethyl ester (FAEE) via the fermentation of sugars liberated from lignocellulosic materials pretreated in acid or alkaline environments are analyzed in terms of economic and environmental metrics. Simplified process models are introduced and employed to estimate process performance, and Monte Carlo analyses were carried out to identify key sources of uncertainty and variability. We find that the near-term performance of processes to produce FAEE is significantly worse than that of ethanol production processes for all metrics considered, primarily due to poor fermentation yields and higher electricity demands for aerobic fermentation. In the longer term, the reduced cost and energy requirements of FAEE separation processes will be at least partially offset by inherent limitations in the relevant metabolic pathways that constrain the maximum yield potential of FAEE from biomass-derived sugars.

  13. The effects of solvent mixtures on the gel permeation chromatography of alkanes, fatty acids, and fatty acid esters 

    E-Print Network [OSTI]

    Bolton, Patrick Lee

    1985-01-01

    with mixtures of 1:1 methanol-chloroform, 1:4 hexane- methylene chloride, 3:2 methylene chloride-acetone, 1:4 methylene chloride-acetone. Improved separation was observed because of enhanced partitioning and adsorption effects that occur with changing...- gold label o-dichlorohenzene- red label eicosane- 99% red label eicosanoic acid- 99% red label hexanoic acid- gold label octadecane- 97+% red label octane- 99+0 gold label octanoic acid- gold label sodium carbonate- gold label A. C. S...

  14. Production of long chain alcohols and alkanes upon coexpression of an acyl-ACP reductase and aldehyde-deformylating oxgenase with a bacterial type-I fatty acid synthase in E. coli

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Coursolle, Dan; Shanklin, John; Lian, Jiazhang; Zhao, Huimin

    2015-06-23

    Microbial long chain alcohols and alkanes are renewable biofuels that could one day replace petroleum-derived fuels. Here we report a novel pathway for high efficiency production of these products in Escherichia coli strain BL21(DE3). We first identified the acyl-ACP reductase/aldehyde deformylase combinations with the highest activity in this strain. Next, we used catalase coexpression to remove toxic byproducts and increase the overall titer. Finally, by introducing the type-I fatty acid synthase from Corynebacterium ammoniagenes, we were able to bypass host regulatory mechanisms of fatty acid synthesis that have thus far hampered efforts to optimize the yield of acyl-ACP-derived products inmore »BL21(DE3). When all these engineering strategies were combined with subsequent optimization of fermentation conditions, we were able to achieve a final titer around 100 mg/L long chain alcohol/alkane products including a 57 mg/L titer of pentadecane, the highest titer reported in E. coli BL21(DE3) to date. The expression of prokaryotic type-I fatty acid synthases offer a unique strategy to produce fatty acid-derived products in E. coli that does not rely exclusively on the endogenous type-II fatty acid synthase system.« less

  15. LONG-CHAIN POLYUNSATURATED FATTY ACID INTAKE AND ITS RELATIONSHIP TO RED BLOOD CELL AND SERUM LONG-CHAIN POLYUNSATURATED FATTY ACIDS IN WOMEN AT HIGH RISK FOR BREAST CANCER

    E-Print Network [OSTI]

    Harvey, Katherine

    2010-04-27

    A higher ratio of omega-6 (n-6) to omega-3 (n-3) long chain polyunsaturated fatty acids (LCPUFA) in breast tissue triglyceride (TG) has been correlated with increased risk of developing breast cancer. Before dietary recommendations can be made...

  16. The Total Fatty Acids and Other Ether-Soluable Constituents of Feedstuffs. 

    E-Print Network [OSTI]

    Rather, J. B. (James Burness)

    1914-01-01

    . Percentage of Fatty Acids in Feedstuffs and Excrements by Various Methods. Labora? tory No. Ether extract. Fatty acids in ether extract. ?a.3 ? g S | . 2 5 a a'-"a _ Differ? ence (B-A) Diges? tion Method. Precipi? tation Method. O O... Q'S'o'cS ? "3 Is E-i . c *= =? a J=! ^ c3 12996 3.79 3.08 0.37 3.45 4.43 0 98 12999 4.31 3.77 0.16 3.93 4.34 0 41 13021 15.23 13.82 0.69 14.51 14.46 -0 05 13023 7.75 6 .2 1 0.39 6.60 8 . 1 0 1 50 13030 3.22 2.05 0.37 2.42 2.92 0 50 13045...

  17. Electrochemical method for producing a biodiesel mixture comprising fatty acid alkyl esters and glycerol

    DOE Patents [OSTI]

    Lin, YuPo J; St. Martin, Edward J

    2013-08-13

    The present invention relates to an integrated method and system for the simultaneous production of biodiesel from free fatty acids (via esterification) and from triglycerides (via transesterification) within the same reaction chamber. More specifically, one preferred embodiment of the invention relates to a method and system for the production of biodiesel using an electrodeionization stack, wherein an ion exchange resin matrix acts as a heterogeneous catalyst for simultaneous esterification and transesterification reactions between a feedstock and a lower alcohol to produce biodiesel, wherein the feedstock contains significant levels of free fatty acid. In addition, because of the use of a heterogeneous catalyst, the glycerol and biodiesel have much lower salt concentrations than raw biodiesel produced by conventional transesterification processes. The present invention makes it much easier to purify glycerol and biodiesel.

  18. Stability and composition of palm, coconut and soy oil fatty acid microemulsion diesel fuels

    SciTech Connect (OSTI)

    Compere, A.L.; Griffith, W.L.; Googin, J.M.

    1985-01-01

    Microemulsions fuels containing fully and partially coconut, palm, and soy fatty acids; varying amounts of C/sub 1/ to C/sub 4/ alcohols; varying amounts of water; and four fuel bases were evaluated between 0 and 60/sup 0/C for stability as a single phase system. In general, ability to form a stable single phase system rose with increasing alcohol chain length, decreasing water, and increasing dispersed phase content. It was possible to form 0 to 60/sup 0/C stable single phase systems in all four fuels tested using 30 to 50% v/v dispersed phase containing 1-butanol and either palm or soy fatty acids. 11 refs., 3 tabs.

  19. Effects of Fatty Acids on Gene Expression and Lipid Metabolism in Bovine Intramuscular and Subcutaneous Adipose Tissues 

    E-Print Network [OSTI]

    Silvey, David Tyrone

    2012-10-19

    Pasture feeding depresses adipose tissue development in beef cattle whereas grain feeding, enhances adipogenesis. Therefore, we hypothesized that specific fatty acids would differentially affect lipogenesis in explants of bovine subcutaneous (SC...

  20. Enrichment of canine gestation and lactation diets with n-3 polyunsaturated fatty acids to support neurologic development 

    E-Print Network [OSTI]

    Heinemann, Kimberly Michele

    2005-11-01

    Long-chain polyunsaturated fatty acids (LCPUFA) are essential for proper neural and retinal development in many mammalian species. One objective of this research was to investigate the effects of dietary ?-linolenic ...

  1. Indices of stress in exercising horses fed diets containing varying amounts of omega-6 and omega-3 polyunsaturated fatty acids 

    E-Print Network [OSTI]

    Howard, Alicia Dawn

    2005-11-01

    -1 INDICES OF STRESS IN EXERCISING HORSES FED DIETS CONTAINING VARYING AMOUNTS OF OMEGA-6 AND OMEGA-3 POLYUNSATURATED FATTY ACIDS A Thesis by ALICIA DAWN HOWARD Submitted to the Office of Graduate Studies of Texas A... OF OMEGA-6 AND OMEGA-3 POLYUNSATURATED FATTY ACIDS A Thesis by ALICIA DAWN HOWARD Submitted to the Office of Graduate Studies of Texas A&M University in partial fulfillment of the requirements for the degree of MASTER...

  2. Inclusion of Blended Lipid Solutions as Functional Ingredients to Alter the Fatty Acid Profile of Beef Patties 

    E-Print Network [OSTI]

    Lowder, Austin C.

    2010-10-12

    affects the nutritive value of meat. Both fatty acids reduce total cholesterol and LDL cholesterol, but n-3 fatty acids provide a host of other health benefits, including being anticarcinogenic, antithrombotic, and antiarrythmic (Leaf and others 2003... antioxidant capacity. Olive oil has also shown to be antithrombotic and preventative of LDL oxidation, as noted by Luruena- Martinez and others (2004) who used olive oil and hydrocolloids to reduce fat levels in frankfurters. Park and others (1989, 1990...

  3. Evaluation of the fatty acid composition and caloric value of ground beef formulated to contain low levels of fat 

    E-Print Network [OSTI]

    Campbell, Laura Elaine

    1988-01-01

    EVALUATION OF THE FATTY ACID COMPOSITION AND CALORIC VALUE OF GROUND BEEF FORMULATED TO CONTAIN LOW LEVELS OF FAT A Thesis by LAURA ELAINE CAMPBELL Submitted to the Graduate College of Texas A&M University in partial fulfillment... of the requirements for the degree of MASTER OF SCIENCE August 1988 Major Subject: Food Science and Technology EVALUATION OF THE FATTY ACID COMPOSITION AND CALORIC VALUE OF GROUND BEEF FORMULATED TO CONTAIN LOW LEVELS OF FAT A Thesis by LAURA ELAINE CAMPBELL...

  4. ANALYSIS OF RICIN TOXIN PREPARATIONS FOR CARBOHYDRATE AND FATTY ACID ABUNDANCE AND ISOTOPE RATIO INFORMATION

    SciTech Connect (OSTI)

    Wunschel, David S.; Kreuzer-Martin, Helen W.; Antolick, Kathryn C.; Colburn, Heather A.; Moran, James J.; Melville, Angela M.

    2009-12-01

    This report describes method development and preliminary evaluation for analyzing castor samples for signatures of purifying ricin. Ricin purification from the source castor seeds is essentially a problem of protein purification using common biochemical methods. Indications of protein purification will likely manifest themselves as removal of the non-protein fractions of the seed. Two major, non-protein, types of biochemical constituents in the seed are the castor oil and various carbohydrates. The oil comprises roughly half the seed weight while the carbohydrate component comprises roughly half of the remaining “mash” left after oil and hull removal. Different castor oil and carbohydrate components can serve as indicators of specific toxin processing steps. Ricinoleic acid is a relatively unique fatty acid in nature and is the most abundant component of castor oil. The loss of ricinoleic acid indicates a step to remove oil from the seeds. The relative amounts of carbohydrates and carbohydrate-like compounds, including arabinose, xylose, myo-inositol fucose, rhamnose, glucosamine and mannose detected in the sample can also indicate specific processing steps. For instance, the differential loss of arabinose relative to mannose and N-acetyl glucosamine indicates enrichment for the protein fraction of the seed using protein precipitation. The methods developed in this project center on fatty acid and carbohydrate extraction from castor samples followed by derivatization to permit analysis by gas chromatography-mass spectrometry (GC-MS). Method descriptions herein include: the source and preparation of castor materials used for method evaluation, the equipment and description of procedure required for chemical derivatization, and the instrument parameters used in the analysis. Two types of derivatization methods describe analysis of carbohydrates and one procedure for analysis of fatty acids. Two types of GC-MS analysis is included in the method development, one employing a quadrupole MS system for compound identification and an isotope ratio MS for measuring the stable isotope ratios of deuterium and hydrogen (D/H) in fatty acids. Finally, the method for analyzing the compound abundance data is included. This study indicates that removal of ricinoleic acid is a conserved consequence of each processing step we tested. Furthermore, the stable isotope D/H ratio of ricinoleic acid distinguished between two of the three castor seed sources. Concentrations of arabinose, xylose, mannose, glucosamine and myo-inositol differentiated between crude or acetone extracted samples and samples produced by protein precipitation. Taken together these data illustrate the ability to distinguish between processes used to purify a ricin sample as well as potentially the source seeds.

  5. Dietary polyunsaturated fatty acids modify plasma lipids and red blood cell membrane composition but do not induce ?6 desaturase mediated conversions in the domestic feline 

    E-Print Network [OSTI]

    McClure, Melena Kathleen

    2009-05-15

    This study explored the effects of dietary unsaturated fatty acids on feline lipid metabolism. It was hypothesized that high dietary linoleic acid (18:2n-6, LA) would enhance conversion to arachidonic acid (20:4n-6), ...

  6. Decreased hepatotoxic bile acid composition and altered synthesis in progressive human nonalcoholic fatty liver disease

    SciTech Connect (OSTI)

    Lake, April D. [University of Arizona, Department of Pharmacology and Toxicology, Tucson, AZ 85721 (United States); Novak, Petr [Biology Centre ASCR, Institute of Plant Molecular Biology, Ceske Budejovice 37001 (Czech Republic); Shipkova, Petia; Aranibar, Nelly; Robertson, Donald; Reily, Michael D. [Pharmaceutical Candidate Optimization, Bristol-Myers Squibb Co., Princeton, NJ 08543 (United States); Lu, Zhenqiang [The Arizona Statistical Consulting Laboratory, University of Arizona, Tucson, AZ 85721 (United States); Lehman-McKeeman, Lois D. [Pharmaceutical Candidate Optimization, Bristol-Myers Squibb Co., Princeton, NJ 08543 (United States); Cherrington, Nathan J., E-mail: cherrington@pharmacy.arizona.edu [University of Arizona, Department of Pharmacology and Toxicology, Tucson, AZ 85721 (United States)

    2013-04-15

    Bile acids (BAs) have many physiological roles and exhibit both toxic and protective influences within the liver. Alterations in the BA profile may be the result of disease induced liver injury. Nonalcoholic fatty liver disease (NAFLD) is a prevalent form of chronic liver disease characterized by the pathophysiological progression from simple steatosis to nonalcoholic steatohepatitis (NASH). The hypothesis of this study is that the ‘classical’ (neutral) and ‘alternative’ (acidic) BA synthesis pathways are altered together with hepatic BA composition during progression of human NAFLD. This study employed the use of transcriptomic and metabolomic assays to study the hepatic toxicologic BA profile in progressive human NAFLD. Individual human liver samples diagnosed as normal, steatosis, and NASH were utilized in the assays. The transcriptomic analysis of 70 BA genes revealed an enrichment of downregulated BA metabolism and transcription factor/receptor genes in livers diagnosed as NASH. Increased mRNA expression of BAAT and CYP7B1 was observed in contrast to decreased CYP8B1 expression in NASH samples. The BA metabolomic profile of NASH livers exhibited an increase in taurine together with elevated levels of conjugated BA species, taurocholic acid (TCA) and taurodeoxycholic acid (TDCA). Conversely, cholic acid (CA) and glycodeoxycholic acid (GDCA) were decreased in NASH liver. These findings reveal a potential shift toward the alternative pathway of BA synthesis during NASH, mediated by increased mRNA and protein expression of CYP7B1. Overall, the transcriptomic changes of BA synthesis pathway enzymes together with altered hepatic BA composition signify an attempt by the liver to reduce hepatotoxicity during disease progression to NASH. - Highlights: ? Altered hepatic bile acid composition is observed in progressive NAFLD. ? Bile acid synthesis enzymes are transcriptionally altered in NASH livers. ? Increased levels of taurine and conjugated bile acids are observed in NASH. ? Hepatic bile acid synthesis shifts toward the alternative pathway in NASH.

  7. Low-Emissions Burner Technology using Biomass-Derived Liquid...

    Broader source: Energy.gov (indexed) [DOE]

    biomass-derived liquid fuels, such as glycerin or fatty acids, as a substitute for natural gas. low-emissionsburnertechnologyfactsheet.pdf More Documents & Publications...

  8. Gene expression profiles of murine fatty liver induced by the administration of valproic acid

    SciTech Connect (OSTI)

    Lee, Min-Ho [College of Pharmacy (Korea, Republic of); Bio-MAX Institute (Korea, Republic of); Hong, Il [College of Pharmacy (Korea, Republic of); Bio-MAX Institute (Korea, Republic of); Kim, Mingoo [Seoul National University Biomedical Informatics College of Medicine (Korea, Republic of); Toxicogenomics Research Consortium, Hanyang University, Seoul 133-791 (Korea, Republic of); Lee, Byung Hoon [College of Pharmacy (Korea, Republic of); Toxicogenomics Research Consortium, Hanyang University, Seoul 133-791 (Korea, Republic of); Kim, Ju-Han [Seoul National University Biomedical Informatics College of Medicine (Korea, Republic of); Toxicogenomics Research Consortium, Hanyang University, Seoul 133-791 (Korea, Republic of); Kang, Kyung-Sun [Department of Veterinary Public Health College of Veterinary Medicine, Seoul National University, Seoul 151-742 (Korea, Republic of); Toxicogenomics Research Consortium, Hanyang University, Seoul 133-791 (Korea, Republic of); Kim, Hyung-Lae [Department of Biochemistry College of Medicine, Ewha Womans University, Seoul 158-710 (Korea, Republic of); Toxicogenomics Research Consortium, Hanyang University, Seoul 133-791 (Korea, Republic of); Yoon, Byung-Il [School of Veterinary Medicine, Kangwon National University, Kangwon 200-701 (Korea, Republic of); Toxicogenomics Research Consortium, Hanyang University, Seoul 133-791 (Korea, Republic of); Chung, Heekyoung [Department of Pathology College of Medicine (Korea, Republic of); Toxicogenomics Research Consortium, Hanyang University, Seoul 133-791 (Korea, Republic of); Kong, Gu [Department of Pathology College of Medicine (Korea, Republic of); Toxicogenomics Research Consortium, Hanyang University, Seoul 133-791 (Korea, Republic of); Lee, Mi-Ock [College of Pharmacy (Korea, Republic of) and Bio-MAX Institute (Korea, Republic of) and Toxicogenomics Research Consortium, Hanyang University, Seoul 133-791 (Korea, Republic of)]. E-mail: molee@snu.ac.kr

    2007-04-01

    Valproic acid (VPA) has been used as anticonvulsants, however, it induces hepatotoxicity such as microvesicular steatosis and necrosis in the liver. To explore the mechanisms of VPA-induced steatosis, we profiled the gene expression patterns of the mouse liver that were altered by treatment with VPA using microarray analysis. VPA was orally administered as a single dose of 100 mg/kg (low-dose) or 1000 mg/kg (high-dose) to ICR mice and the animals were killed at 6, 24, or 72 h after treatment. Serum alanine aminotransferase and aspartate aminotransferase levels were not significantly altered in the experimental animals. However, symptoms of steatosis were observed at 72 h with low-dose and at 24 h and 72 h with high-dose. After microarray data analysis, 1910 genes were selected by two-way ANOVA (P < 0.05) as VPA-responsive genes. Hierarchical clustering revealed that gene expression changes depended on the time rather than the dose of VPA treatment. Gene profiling data showed striking changes in the expression of genes associated with lipid, fatty acid, and steroid metabolism, oncogenesis, signal transduction, and development. Functional categorization of 1156 characteristically up- and down-regulated genes (cutoff > 1.5-fold) revealed that 60 genes were involved in lipid metabolism that was interconnected with biological pathways for biosynthesis of triglyceride and cholesterol, catabolism of fatty acid, and lipid transport. This gene expression profile may be associated with the known steatogenic hepatotoxicity of VPA and it may provide useful information for prediction of hepatotoxicity of unknown chemicals or new drug candidates through pattern recognition.

  9. Impact of Herbicides on Winter Canola (Brassica napus L.) Production and Fatty Acid Composition in South Texas 

    E-Print Network [OSTI]

    Cogdill, Todd Joseph

    2013-01-16

    to evaluate a broad spectrum of herbicides for potential use in South Texas canola production with respect to crop injury, effects on canola seed oil content, fatty acid composition, weed control, biomass yield, and forage quality. Visual crop injury at 42...

  10. Enrichment of plasma lipid subfractions with varying dietary levels of N-3 fatty acids in the canine model 

    E-Print Network [OSTI]

    Spencer, Angela Leah

    1999-01-01

    In an attempt to better understand how varying levels of dietary n-3 fatty acids affected enrichment of plasma lipid subfractions, the current study was undertaken. Adult dogs were fed one of two basal diets with the addition of beef tallow...

  11. The selective effect of dietary n-3 polyunsaturated fatty acids on murine Th1 and Th2 cell development 

    E-Print Network [OSTI]

    Zhang, Ping

    2006-10-30

    To examine how dietary n-3 polyunsaturated fatty acids affect Th2 cell development, female C57BL/6 mice were fed a washout corn oil (CO) diet for 1 wk followed by 2 wk of either the same CO diet or a fish oil (FO) diet. CD4+ T cells were isolated...

  12. The influence of molecular structure of fatty acid monoalkyl esters on diesel combustion

    SciTech Connect (OSTI)

    Schoenborn, Alessandro; Ladommatos, Nicos [Department of Mechanical Engineering, University College London, Torrington Place, London WC1E 7JE (United Kingdom); Williams, John; Allan, Robert; Rogerson, John [BP Oil UK Limited, Technology Centre, Whitchurch Hill, Pangbourne, Reading, RG8 7QR (United Kingdom)

    2009-07-15

    The subject of this paper is a series of experiments conducted on a single-cylinder research engine investigating the influence of molecular structure on the combustion behaviour of fatty acid alcohol ester (biodiesel) molecules under diesel engine conditions. The fuels employed in these experiments comprised various samples of pure individual fatty acid alcohol ester molecules of different structure, as well as several mixtures of such molecules. The latter consisted in biodiesel fuels produced by the transesterification of naturally occurring plant oils or animal fat with a monohydric alcohol. It was observed that the molecular structure of the fuel significantly influenced the formation of NO{sub x} and particulate matter and their respective concentration in the exhaust gas. The influence on the formation of NO{sub x} in particular, appeared to be exerted first through the effect which the molecular structure had on the auto-ignition delay occurring after the fuel was injected into the combustion chamber, and second through the flame temperature at which the various molecules burned. The emission of particulates on the other hand showed correlation with the number of double bonds in the fuel molecules for the case of larger accumulation mode particles, and with the boiling point of the fuel samples for the case of the smaller, nucleation mode particles. The effect of ignition delay on the exhaust emissions of these pollutants was isolated by adding the ignition promoting molecule 2-ethylhexyl nitrate to some of the fuel samples in closely specified concentrations, so as to equalise the ignition delay for the relevant fuel samples. The removal of the ignition delay as a main influence on the combustion process enabled the observation of the lesser effects of adiabatic flame temperature. (author)

  13. Genome-wide analysis of the omega-3 fatty acid desaturase gene family in Gossypium

    SciTech Connect (OSTI)

    Yurchenko, Olga P.; Park, Sunjung; Ilut, Daniel C.; Inmon, Jay J.; Millhollon, Jon C.; Liechty, Zach; Page, Justin T.; Jenks, Matthew A.; Chapman, Kent D.; Udall, Joshua A.; Gore, Michael A.; Dyer, John M.

    2014-11-18

    The majority of commercial cotton varieties planted worldwide are derived from Gossypium hirsutum, which is a naturally occurring allotetraploid produced by interspecific hybridization of A- and D-genome diploid progenitor species. While most cotton species are adapted to warm, semi-arid tropical and subtropical regions, and thus perform well in these geographical areas, cotton seedlings are sensitive to cold temperature, which can significantly reduce crop yields. One of the common biochemical responses of plants to cold temperatures is an increase in omega-3 fatty acids, which protects cellular function by maintaining membrane integrity. The purpose of our study was to identify and characterize the omega-3 fatty acid desaturase (FAD) gene family in G. hirsutum, with an emphasis on identifying omega-3 FADs involved in cold temperature adaptation. Results: Eleven omega-3 FAD genes were identified in G. hirsutum, and characterization of the gene family in extant A and D diploid species (G. herbaceum and G. raimondii, respectively) allowed for unambiguous genome assignment of all homoeologs in tetraploid G. hirsutum. The omega-3 FAD family of cotton includes five distinct genes, two of which encode endoplasmic reticulum-type enzymes (FAD3-1 and FAD3-2) and three that encode chloroplast-type enzymes (FAD7/8-1, FAD7/8-2, and FAD7/8-3). The FAD3-2 gene was duplicated in the A genome progenitor species after the evolutionary split from the D progenitor, but before the interspecific hybridization event that gave rise to modern tetraploid cotton. RNA-seq analysis revealed conserved, gene-specific expression patterns in various organs and cell types and semi-quantitative RT-PCR further revealed that FAD7/8-1 was specifically induced during cold temperature treatment of G. hirsutum seedlings. Conclusions: The omega-3 FAD gene family in cotton was characterized at the genome-wide level in three species, showing relatively ancient establishment of the gene family prior to the split of A and D diploid progenitor species. The FAD genes are differentially expressed in various organs and cell types, including fiber, and expression of the FAD7/8-1 gene was induced by cold temperature. These data define the genetic and functional genomic properties of this important gene family in cotton and provide a foundation for future efforts to improve cotton abiotic stress tolerance through molecular breeding approaches.

  14. Extracting metal ions with diphosphonic acid, or derivative thereof

    DOE Patents [OSTI]

    Horwitz, Earl P. (Argonne, IL); Gatrone, Ralph C. (Argonne, IL); Nash, Kenneth L. (Argonne, IL)

    1994-01-01

    Thermodynamically-unstable complexing agents which are diphosphonic acids and diphosphonic acid derivatives (or sulphur containing analogs), like carboxyhydroxymethanediphosphonic acid and vinylidene-1,1-diphosphonic acid, are capable of complexing with metal ions, and especially metal ions in the II, III, IV, V and VI oxidation states, to form stable, water-soluble metal ion complexes in moderately alkaline to highly-acidic media. However, the complexing agents can be decomposed, under mild conditions, into non-organic compounds which, for many purposes are environmentally-nondamaging compounds thereby degrading the complex and releasing the metal ion for disposal or recovery. Uses for such complexing agents as well as methods for their manufacture are also described.

  15. Membrane extraction with thermodynamically unstable diphosphonic acid derivatives

    DOE Patents [OSTI]

    Horwitz, E.P.; Gatrone, R.C.; Nash, K.L.

    1997-10-14

    Thermodynamically-unstable complexing agents which are diphosphonic acids and diphosphonic acid derivatives (or sulphur containing analogs), like carboxyhydroxymethanediphosphonic acid and vinylidene-1,1-diphosphonic acid, are capable of complexing with metal ions, and especially metal ions in the II, III, IV, V and VI oxidation states, to form stable, water-soluble metal ion complexes in moderately alkaline to highly-acidic media. However, the complexing agents can be decomposed, under mild conditions, into non-organic compounds which, for many purposes are environmentally-nondamaging compounds thereby degrading the complex and releasing the metal ion for disposal or recovery. Uses for such complexing agents as well as methods for their manufacture are also described. 1 fig.

  16. Membrane extraction with thermodynamically unstable diphosphonic acid derivatives

    DOE Patents [OSTI]

    Horwitz, Earl Philip (Argonne, IL); Gatrone, Ralph Carl (Argonne, IL); Nash, Kenneth LaVerne (Argonne, IL)

    1997-01-01

    Thermodynamically-unstable complexing agents which are diphosphonic acids and diphosphonic acid derivatives (or sulphur containing analogs), like carboxyhydroxymethanediphosphonic acid and vinylidene-1,1-diphosphonic acid, are capable of complexing with metal ions, and especially metal ions in the II, III, IV, V and VI oxidation states, to form stable, water-soluble metal ion complexes in moderately alkaline to highly-acidic media. However, the complexing agents can be decomposed, under mild conditions, into non-organic compounds which, for many purposes are environmentally-nondamaging compounds thereby degrading the complex and releasing the metal ion for disposal or recovery. Uses for such complexing agents as well as methods for their manufacture are also described.

  17. Extracting metal ions with diphosphonic acid, or derivative thereof

    DOE Patents [OSTI]

    Horwitz, E.P.; Gatrone, R.C.; Nash, K.L.

    1994-07-26

    Thermodynamically-unstable complexing agents which are diphosphonic acids and diphosphonic acid derivatives (or sulfur containing analogs), like carboxyhydroxymethanediphosphonic acid and vinylidene-1,1-diphosphonic acid, are capable of complexing with metal ions, and especially metal ions in the II, III, IV, V and VI oxidation states, to form stable, water-soluble metal ion complexes in moderately alkaline to highly-acidic media. However, the complexing agents can be decomposed, under mild conditions, into non-organic compounds which, for many purposes are environmentally-nondamaging compounds thereby degrading the complex and releasing the metal ion for disposal or recovery. Uses for such complexing agents as well as methods for their manufacture are also described. 1 fig.

  18. Accurate and Reliable Quantification of Total Microalgal Fuel Potential as Fatty Acid Methyl Esters by in situ Transesterfication

    SciTech Connect (OSTI)

    Laurens, L. M. L.; Quinn, M.; Van Wychen, S.; Templeton, D. W.; Wolfrum, E. J.

    2012-04-01

    In the context of algal biofuels, lipids, or better aliphatic chains of the fatty acids, are perhaps the most important constituents of algal biomass. Accurate quantification of lipids and their respective fuel yield is crucial for comparison of algal strains and growth conditions and for process monitoring. As an alternative to traditional solvent-based lipid extraction procedures, we have developed a robust whole-biomass in situ transesterification procedure for quantification of algal lipids (as fatty acid methyl esters, FAMEs) that (a) can be carried out on a small scale (using 4-7 mg of biomass), (b) is applicable to a range of different species, (c) consists of a single-step reaction, (d) is robust over a range of different temperature and time combinations, and (e) tolerant to at least 50% water in the biomass. Unlike gravimetric lipid quantification, which can over- or underestimate the lipid content, whole biomass transesterification reflects the true potential fuel yield of algal biomass. We report here on the comparison of the yield of FAMEs by using different catalysts and catalyst combinations, with the acid catalyst HCl providing a consistently high level of conversion of fatty acids with a precision of 1.9% relative standard deviation. We investigate the influence of reaction time, temperature, and biomass water content on the measured FAME content and profile for 4 different samples of algae (replete and deplete Chlorella vulgaris, replete Phaeodactylum tricornutum, and replete Nannochloropsis sp.). We conclude by demonstrating a full mass balance closure of all fatty acids around a traditional lipid extraction process.

  19. Lipid Metabolism, Learning Ability and Potential Biomarkers for Atherosclerosis in Monk Parrots (Myiopsitta monachus) Fed N-3 Fatty Acids 

    E-Print Network [OSTI]

    Petzinger, Christina

    2012-07-16

    Dissertation by CHRISTINA A PETZINGER Submitted to the Office of Graduate Studies of Texas A&M University in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY Approved by: Co-Chairs of Committee, John E. Bauer J... and Potential Biomarkers for Atherosclerosis in Monk Parrots (Myiopsitta monachus) Fed N-3 Fatty Acids. (May 2012) Christina A Petzinger, B.S., Berry College Co-Chairs of Advisory Committee: Dr. John E. Bauer Dr. J. Jill Heatley...

  20. Functional screening and in vitro analysis reveals thioesterases with enhanced substrate specificity profiles that improve short-chain fatty acid production in Escherichia coli

    E-Print Network [OSTI]

    Prather, Kristala L. Jones

    2014-01-01

    Short-chain fatty acid (SCFAs) biosynthesis is pertinent to production of biofuels, industrial compounds, and pharmaceuticals from renewable resources. To expand on Escherichia coli SCFA products, we previously implemented ...

  1. Carcass characteristics, fatty acids, stearoyl-coa desaturase gene expression and sensory evaluation of calf-fed and yearling-fed angus steers 

    E-Print Network [OSTI]

    Brooks, Matthew Alan

    2009-05-15

    of the digesta is not only a function of the dietary fat intake but also of the microbial manipulation and production of fatty acids. 6 69 The specific microbes and controls in the rumen that facilitate biohydrogenation remain undetermined. This also... by the beef industry in providing a healthier product, it is apparent that different feeding strategies can lead to alterations in the quality of the fatty acid composition in beef. The beef industry can optimize their management strategies to raise cattle...

  2. Effect of Conjugated Linoleic Acid or Oleic Acid Addition on Fatty Acid Composition Profiles of Poultry Meat 

    E-Print Network [OSTI]

    Shin, Dae Keun

    2011-08-08

    and fish oil diet had a lower C20:4 (arachidonic acid, AA, n-6) deposition but showed a higher n-3/n-6 ratio in breast and thigh meat than those fed a flaxseed oil diet and CLA and flaxseed oil diet (P < 0.05). The C20:4 and n-3/n-6 ratio of breast...

  3. Investigating fatty acid biosynthesis within the algal chloroplast using Chlamydomonas reinhardtii as a model

    E-Print Network [OSTI]

    Blatti, Jillian L.

    2012-01-01

    acid biosynthesis in microalgae for sustainable biodiesel. (acid biosynthesis in microalgae for biofuel through protein-acid biosynthesis in microalgae through protein-protein

  4. Identification of 13-Hydroxy-14,15-epoxyeicosatrienoic Acid as an Acid-stable Endothelium-derived Hyperpolarizing

    E-Print Network [OSTI]

    Hammock, Bruce D.

    Identification of 13-Hydroxy-14,15-epoxyeicosatrienoic Acid as an Acid-stable Endothelium-derived and vasoconstric- tor autacoids. Two of the well-defined endothelium-derived vasodilators are prostacyclin (PGI2, this third factor is termed endothelium-derived hyperpo- larizing factor (EDHF) (7­10). Unlike PGI2 and NO

  5. Synthesis of amino acid derivatives of indole-3-acetic acid Ying Liu*, Liang Zhao, Liang Liu, Lin-Yi Wei and Lu-Hua Lai*

    E-Print Network [OSTI]

    Luhua, Lai

    Synthesis of amino acid derivatives of indole-3-acetic acid Ying Liu*, Liang Zhao, Liang Liu, Lin, Beijing, 100871, China Amino acid derivatives of a modified indole-3-acetic acid have been synthesised on the IR, 1H NMR, MS spectra. Keywords: indole-3-acetic acid, amino acid, dipeptide-like compound Indole-3

  6. Energy densification of biomass-derived organic acids

    DOE Patents [OSTI]

    Wheeler, M. Clayton; van Walsum, G. Peter; Schwartz, Thomas J.; van Heiningen, Adriaan

    2013-01-29

    A process for upgrading an organic acid includes neutralizing the organic acid to form a salt and thermally decomposing the resulting salt to form an energy densified product. In certain embodiments, the organic acid is levulinic acid. The process may further include upgrading the energy densified product by conversion to alcohol and subsequent dehydration.

  7. UNCORRECTEDPROOF Please cite this article in press as: D. Lipkind et al., The vaporization enthalpies and vapor pressures of a series of unsaturated fatty acids methyl

    E-Print Network [OSTI]

    Chickos, James S.

    enthalpies and vapor pressures of a series of unsaturated fatty acids methyl esters by correlation gas­8 Thermochimica Acta xxx (2007) xxx­xxx The vaporization enthalpies and vapor pressures of a series of unsaturated- vonate (methyl Z 15-tetracosenoate) are evaluated at T = 298.15 and vapor pressures are evaluated over

  8. Sources and distribution of CuO-derived benzene carboxylic acids in soils and sediments

    E-Print Network [OSTI]

    Long, Bernard

    Sources and distribution of CuO-derived benzene carboxylic acids in soils and sediments Angela F vas- cular plant-derived OC, through the environment. The method produces a suite of benzene

  9. Constructing and engineering fatty acid metabolic pathways for the production of fuels and chemicals

    E-Print Network [OSTI]

    Steen, Eric James

    2010-01-01

    of alternative energy and petroleum sources. Anotherto petroleum-derived fuel may provide energy sustainabilitypetroleum,” being a primary metabolite used by cells for both chemical and energy

  10. ORIGINAL ARTICLE Fatty Acid Profile of Sunshine Bass: I. Profile Change is Affected

    E-Print Network [OSTI]

    ) or a 50:50 blend of FO and coconut (CO), grapeseed (GO), linseed (LO), or poultry (PO) oil. For the final. Abbreviations ANOVA Analysis of variance CO Coconut oil DHA Docosahexaenoic acid, 22:6n-3 EPA Eicosapentaenoic

  11. Compound-Specific Radiocarbon Analyses of Phospholipid Fatty Acids and n-Alkanes in Ocean Sediments

    E-Print Network [OSTI]

    2010-01-01

    Monica Basin surface sediment: a model based on compound-ACIDS AND n-ALKANES IN OCEAN SEDIMENTS Ellen R M Druffel 1 •organic matter in ocean sediments from the northeast Pacific

  12.  COMPOUND-SPECIFIC RADIOCARBON ANALYSES OF PHOSPHOLIPID FATTY ACIDS AND n -ALKANES IN OCEAN SEDIMENTS

    E-Print Network [OSTI]

    2010-01-01

    Monica Basin surface sediment: a model based on compound-ACIDS AND n-ALKANES IN OCEAN SEDIMENTS Ellen R M Druffel 1 •organic matter in ocean sediments from the northeast Pacific

  13. The role of Ncb5or in fatty acid metabolism and redox homeostasis

    E-Print Network [OSTI]

    Xu, Ming

    2011-12-31

    response gene expression, oxidized glutathione content and cell death. These phenotypes could be alleviated by co-incubation with oleic acid via TAG channeling. Taken together, these findings suggest that increased FFA accumulation and catabolism...

  14. On the nature of the deactivation of supported palladium nanoparticle catalysts in the decarboxylation of fatty acids.

    SciTech Connect (OSTI)

    Ping, E. W.; Pierson, J.; Wallace, R.; Miller, J. T.; Fuller, T. F.; Jones, C. W.

    2011-04-15

    Supported palladium catalysts are effective catalysts for the hydrogen-free decarboxylation of fatty acids. However, the catalysts deactivate severely after one use. Here, the recyclability of a well-defined, mesoporous silica-supported palladium nanoparticle catalyst is evaluated in the batch decarboxylation of stearic acid at 300 C under inert atmosphere, producing n-heptadecane. The nature of the catalyst deactivation is examined in detail via an array of characterization techniques. X-ray photoelectron spectroscopy (XPS) demonstrates that little palladium surface oxidation occurs over the course of the reaction, and a combination of X-ray absorption spectroscopy and transmission electron microscopy (TEM) suggests negligible particle sintering or agglomeration. Physisorption and chemisorption measurements demonstrate substantial loss in total surface area and porosity as well as accessible palladium surface area with these losses attributed to significant organic deposition on the catalyst, as verified via thermogravimetric analysis. High temperature calcination is applied to combust and remove these residues, but resultant nanoparticle agglomeration is significant. Solid state nuclear magnetic resonance spectroscopy (NMR), Fourier transform infrared spectroscopy (FT-IR) and solid dissolution followed by organic extraction methodologies demonstrate that the carbonaceous deposits are not coke but rather strongly adsorbed reactants and products. Detrimental coke formation, as suggested by prior literature, is verified to be absent, as extraction of the surface-deposited organic species yields nearly complete recovery of the total surface area, pore volume, and active palladium surface area. Furthermore, the regenerated catalyst exhibits a corresponding significant recovery of decarboxylation activity.

  15. Fatty acid composition in the mature milk of Bolivian forager-horticulturalists: controlled comparisons with a

    E-Print Network [OSTI]

    Gurven, Michael

    comparisons with a US samplemcn_412 404..418 Melanie A. Martin*, William D. Lassek , Steven J.C. Gaulin, arachidonic acid, breast milk, infant and child nutrition. Correspondence: Melanie A. Martin, Integrative affecting the percent- ages of many specific FAs, including DHA, in milk (Jensen 1999; Innis 2007b). FAs

  16. Asymmetric Hydrogenation of Itaconic Acid and Enol Acetate Derivatives with

    E-Print Network [OSTI]

    Zhang, Xumu

    of acyclic enol acetates bearing aromatic substituents. Chiral 2-substituted succinic acids have attracted. When methanol was used as the reaction solvent, the hydrogenation product (S)-dimethyl 2

  17. A study of human plasma lipid and lipoprotein responses to a diet high in oleic acid derived from pecans 

    E-Print Network [OSTI]

    Barloon, Jessica Lynn

    2001-01-01

    . Dietary compliance was excellent as judged by changes in proportions of n-9 to n-7 fatty acids within individual plasma lipid classes. Serum lipids, density fractionated lipoproteins, apolipoproteins (Apo B100, Apo AI, Lp(a)), and acute phase proteins...

  18. Effects of stress on serum triglycerides, nonsterified fatty acids, and total cholesterol levels in male rats after ethanol administration

    SciTech Connect (OSTI)

    Hershock, D.; Vogel, W.H. (Thomas Jefferson Univ., Philadelphia, PA (USA))

    1989-02-09

    Serum triglycerides, nonesterified fatty acids (NEFA), and total cholesterol were determined during one hour immobilization stress in adult male Sprague-Dawley rats after ethanol administration (2g/kg, i.p.). Stress and ethanol effects were evaluated in two experiments: (1) rats maintained on Purina Rodent Chow for six weeks and fasted for 24 hours; and (2) rats maintained on the same diet supplemented with 1% cholesterol and 10% peanut oil for six weeks and nonfasted prior to experimentation. Blood was obtained from indwelling jugular catheters. In each experiment, differences were seen in triglyceride and NEFA levels but not in total cholesterol. In the regular diet-fed rats (1), serum triglyceride levels were not affected by either stress or ethanol. However, NEFA levels did show differences in the response to ethanol and stress. A 63% decrease from baseline after 5{prime} of stress was partially abolished by ethanol; instead, a 24% increase was observed. Also, a stress-induced increase in NEFA which occurred after 15{prime} was not observed in the ethanol treated rats; rather, a decrease in NEFA was noted. Total cholesterol did not change in response to stress or ethanol. In the high cholesterol diet-fed rats (2), ethanol did not suppress a stress-induced increase in triglyceride levels. NEFA levels in ethanol-treated rats were higher during the first 15{prime} of stress as compared to stress alone. A decrease in NEFA was however seen in the ethanol-treated rats after 30{prime} of stress and these levels remained lower than the stress alone group. A diet-induced increase in total cholesterol levels was observed; however, no changes were seen due to either or ethanol. Thus, ethanol administration prior to acute immobilization stress did affect serum triglyceride and NEFA levels but did not change total cholesterol.

  19. Technological and economic potential of poly(lactic acid) and lactic acid derivatives

    SciTech Connect (OSTI)

    Datta, R.; Tsai, S.P.; Bonsignore, P.; Moon, S.H.; Frank, J.R.

    1993-10-01

    Lactic acid has been an intermediate-volume specialty chemical (world production {approximately}40,000 tons/yr) used in a wide range of food processing and industrial applications. lactic acid h,as the potential of becoming a very large volume, commodity-chemical intermediate produced from renewable carbohydrates for use as feedstocks for biodegradable polymers, oxygenated chemicals, plant growth regulators, environmentally friendly ``green`` solvents, and specially chemical intermediates. In the past, efficient and economical technologies for the recovery and purification of lactic acid from crude fermentation broths and the conversion of tactic acid to the chemical or polymer intermediates had been the key technology impediments and main process cost centers. The development and deployment of novel separations technologies, such as electrodialysis (ED) with bipolar membranes, extractive distillations integrated with fermentation, and chemical conversion, can enable low-cost production with continuous processes in large-scale operations. The use of bipolar ED can virtually eliminate the salt or gypsum waste produced in the current lactic acid processes. In this paper, the recent technical advances in tactic and polylactic acid processes are discussed. The economic potential and manufacturing cost estimates of several products and process options are presented. The technical accomplishments at Argonne National Laboratory (ANL) and the future directions of this program at ANL are discussed.

  20. The effect of different levels of forage and fish meal on the live performance and rumen volatile fatty acid concentation of heifers fed high molasses diets 

    E-Print Network [OSTI]

    Estrada, Sergio

    1973-01-01

    THE EFFECT OF DIFFERENT LEVELS OF FORAGE AND FISH MEAL ON THE LIVE PERFORMANCE AND RUMEN VOLATILE FATTY ACID CONCEN- TRATION OF HEIFERS FED HIGH MOLASSES DIETS A Thesis by SERGIO ESTRADA Submitted to the Graduate College of Texas ASM...- TRATION OF HEIFERS FED HIGH MOLASSES DIETS A Thesis by SERGIO ESTRADA Approved as to style and content by: ) / Chairman of i tee Member Head of Department Member August 1973 4 3 6 8 1 5 AB STRACT The Effect of Different Levels of Forage and Fish...

  1. Scaleable production and separation of fermentation-derived acetic acid. Final CRADA report.

    SciTech Connect (OSTI)

    Snyder, S. W.; Energy Systems

    2010-02-08

    Half of U.S. acetic acid production is used in manufacturing vinyl acetate monomer (VAM) and is economical only in very large production plants. Nearly 80% of the VAM is produced by methanol carbonylation, which requires high temperatures and exotic construction materials and is energy intensive. Fermentation-derived acetic acid production allows for small-scale production at low temperatures, significantly reducing the energy requirement of the process. The goal of the project is to develop a scaleable production and separation process for fermentation-derived acetic acid. Synthesis gas (syngas) will be fermented to acetic acid, and the fermentation broth will be continuously neutralized with ammonia. The acetic acid product will be recovered from the ammonium acid broth using vapor-based membrane separation technology. The process is summarized in Figure 1. The two technical challenges to success are selecting and developing (1) microbial strains that efficiently ferment syngas to acetic acid in high salt environments and (2) membranes that efficiently separate ammonia from the acetic acid/water mixture and are stable at high enough temperature to facilitate high thermal cracking of the ammonium acetate salt. Fermentation - Microbial strains were procured from a variety of public culture collections (Table 1). Strains were incubated and grown in the presence of the ammonium acetate product and the fastest growing cultures were selected and incubated at higher product concentrations. An example of the performance of a selected culture is shown in Figure 2. Separations - Several membranes were considered. Testing was performed on a new product line produced by Sulzer Chemtech (Germany). These are tubular ceramic membranes with weak acid functionality (see Figure 3). The following results were observed: (1) The membranes were relatively fragile in a laboratory setting; (2) Thermally stable {at} 130 C in hot organic acids; (3) Acetic acid rejection > 99%; and (4) Moderate ammonia flux. The advantages of producing acetic acid by fermentation include its appropriateness for small-scale production, lower cost feedstocks, low energy membrane-based purification, and lower temperature and pressure requirements. Potential energy savings of using fermentation are estimated to be approximately 14 trillion Btu by 2020 from a reduction in natural gas use. Decreased transportation needs with regional plants will eliminate approximately 200 million gallons of diesel consumption, for combined savings of 45 trillion Btu. If the fermentation process captures new acetic acid production, savings could include an additional 5 trillion Btu from production and 7 trillion Btu from transportation energy.

  2. Characterization and CPDM modeling of volatile fatty acid fermentation with cotton gin trash and chicken manrure as substrates 

    E-Print Network [OSTI]

    Han, Joseph

    2013-02-22

    . Maximum product concentrations when using GT and CM at a temperature of 55?C were 22 g of total acid/L with acetic acid constituting 78 % of the total acids. The Continuum Particle Distribution Modeling (CPDM) method is also applied to data collected...

  3. THE COMPETITION BETWEEN METHYLMERCURY RISKS AND OMEGA-3 POLYUNSATURATED FATTY ACID BENEFITS: A REVIEW OF CONFLICTING EVIDENCE ON FISH CONSUMPTION AND CARDIOVASCULAR HEALTH.

    SciTech Connect (OSTI)

    LIPFERT, F.W.; SULLIVAN, T.M.

    2006-10-31

    The health concerns of methylmercury (MeHg) contamination of seafood have recently been extended to include cardiovascular effects, especially premature mortality. Although the fatty acids (fish oils) found in most species are thought to confer a wide range of health benefits, especially to the cardiovascular system, some epidemiological studies have suggested that such benefits may be offset by adverse effects of MeHg. This comprehensive review is based on searches of the NIH MEDLINE database and compares and contrasts 145 published studies involving cardiovascular effects and exposures to mercury and other fish contaminants, intake of fatty acids including dietary supplements of fish oils, and rates of seafood consumption. Since few of these studies include adequate simultaneous measurements of all of these potential predictor variables, we summarized their effects separately, across the available studies of each, and then drew conclusions based on the aggregated findings. It is important to realize that studies of seafood consumption encompass the net effects of all of these predictor variables, but that seafood intake studies are rarely supported by human biomarker measurements that reflect the actual uptake of harmful as well as beneficial fish ingredients. As a result, exposure measurement error is an issue when comparing studies and predictor variables. It is also possible that the observed benefits of eating fish may relate more to the characteristics of the consumers than to those of the fish. We found the evidence for adverse cardiovascular effects of MeHg to be sparse and unconvincing. Studies of cardiovascular mortality show net benefits, and the findings of adverse effects are mainly limited to studies Finland at high mercury exposure levels. By contrast, a very consistent picture of beneficial effects is seen for fatty acids, after recognizing the effects of exposure uncertainties and the presence of threshold effects. Studies based on measured biomarker levels are seen to be the most reliable and present a convincing picture of strong beneficial effects, especially for those causes of death involving cardiac arrhythmia. This conclusion also extends to studies of fish-oil supplementation. Studies based on fish consumption show mainly benefits from increased consumption. This finding is supported by an ecological study at the national population level, for which the lifestyle effects that might be correlated with fish consumption within a given population would be expected to ''average out'' across nations. Finally, the net survival benefits resulting from eating fish are consistent with studies involving complete diets, although benefits are also seen to accrue from reduced consumption of red meat and saturated fats.

  4. Exploring the associations of maternal red blood cell fatty acids, infant body composition, and quality of infant growth

    E-Print Network [OSTI]

    Newbold, Emily A.

    2014-05-31

    studies (17). Vaccenic acid Vaccenic acid (18:1n-7t) is a natural monoene isomer of oleic acid (18:1) and is the major trans isomer in ruminant fats (17). It is created by the incomplete microbial biohydrogenation of linoleic acid (LA) (18:2 n-6... that an organic diet can produce significantly greater levels of vaccenic acid and CLA in breast milk compared to consuming a similar diet of conventional products (18). In this pilot study, women who consumed 50% or greater energy from organic meat and dairy...

  5. A randomised controlled trial investigating the effect of n-3 long-chain polyunsaturated fatty acid supplementation on cognitive and retinal function in cognitively healthy older people: the Older People And n-3 Long-chain polyunsaturated fatty acids (OPAL) study protocol [ISRCTN72331636

    E-Print Network [OSTI]

    Dangour, Alan D.; Clemens, Felicity; Elbourne, Diana; Fasey, Nicky; Fletcher, Astrid E.; Hardy, Pollyanna; Holder, Graham E.; Huppert, Felicia A.; Knight, Rosemary; Letley, Louise; Richards, Marcus; Truesdale, Ann; Vickers, Madge; Uauy, Ricardo

    2006-08-31

    -chain polyunsaturated fatty acids (OPAL) study protocol [ISRCTN72331636] Alan D Dangour*1, Felicity Clemens2, Diana Elbourne2, Nicky Fasey3, Astrid E Fletcher4, Pollyanna Hardy2,5, Graham E Holder6, Felicia A Huppert7, Rosemary Knight3, Louise Letley3, Marcus... will be identical in size, shape, colour and smell for both the intervention and placebo arms of the trial. Given the lack of trial evi- dence to support the use of any particular level of dietary supplementation, the dose selected in the OPAL study was based...

  6. Effect of consuming ground beef of differing monounsaturated fatty acid content on atherosclerotic cardiovascular disease risk factors in healthy men 

    E-Print Network [OSTI]

    Cao, Xiaojuan

    2009-05-15

    Atherosclerotic cardiovascular disease (ASCVD) is currently the most common cause of death in the United States. Some dietary factors contribute importantly to ASCVD and other factors can reduce risk of ASCVD. Oleic acid ...

  7. A Direct, Biomass-Based Synthesis of Benzoic Acid: Formic Acid-Mediated Deoxygenation of the Glucose-Derived Materials Quinic Acid and Shikimic Acid

    E-Print Network [OSTI]

    2010-01-01

    R.G.B and J.A.E. ). Keywords: biomass · carboxylic acids ·10.1002/cssc.201000111 A Direct, Biomass-Based Synthesis ofaro- matic compounds from biomass resources could provide a

  8. Effects of a High Oleic Acid Beef Diet on Cardiovascular Disease Risk Factors of Human Subjects 

    E-Print Network [OSTI]

    Adams, Thaddeus Hunter

    2012-10-19

    The consumption of high-fat hamburger enriched with saturated fatty acids (SFA) and trans-fatty acids (TFA) may increase risk factors for cardiovascular disease, whereas hamburger enriched with monounsaturated fatty acids ...

  9. A novel, easily synthesized, anhydrous derivative of phosphoric acid for use with electrolyte in phosphoric acid-based fuel cells.

    E-Print Network [OSTI]

    Angell, C. Austen

    that of any other type of fuel cell operating at 1 atm. pressure. Its voltage efficiency is also comparable in phosphoric acid-based fuel cells. Younes Ansari, Telpriore Tucker, and C. Austen Angell* Dept. of Chemistry as a fuel cell electrolyte, by designing a variant of the molecular acid that has increased temperature

  10. Oligonucleoside alkyl or arylphosphonate derivatives capable of crosslinking with or cleaving nucleic acids

    DOE Patents [OSTI]

    Miller, Paul S. (Baltimore, MD); Ts'o, Paul O.P. (Lutherville, MD)

    1999-06-15

    A composition for inactivating a target nucleic acid which comprises an oligonucleoside alkyl or arylphosphonate analogue which is complementary to the sequence of the target nucleic acid and includes a functional group which reacts with the target nucleic acid to render the target nucleic acid inactive or nonfunctional.

  11. Oligonucleoside alkyl or arylphosphonate derivatives capable of crosslinking with or cleaving nucleic acids

    DOE Patents [OSTI]

    Miller, P.S.; Ts'o, P.O.P.

    1999-06-15

    A composition for inactivating a target nucleic acid which comprises an oligonucleoside alkyl or arylphosphonate analogue which is complementary to the sequence of the target nucleic acid is provided. It includes a functional group which reacts with the target nucleic acid to render the target nucleic acid inactive or nonfunctional. 16 figs.

  12. Fuel and lubricant additives from acid treated mixtures of vegetable oil derived amides and esters

    SciTech Connect (OSTI)

    Bonazza, B.R.; Devault, A.N.

    1981-05-26

    Vegetable oils such as corn oil, peanut oil, and soy oil are reacted with polyamines to form a mixture containing amides, imides, half esters, and glycerol with subsequent treatment with a strong acid such as sulfonic acid to produce a product mix that has good detergent properties in fuels and lubricants.

  13. Study on reduction and back extraction of Pu(IV) by urea derivatives in nitric acid conditions

    SciTech Connect (OSTI)

    Ye, G.A.; Xiao, S.T.; Yan, T.H.; Lin, R.S.; Zhu, Z.W.

    2013-07-01

    The reduction kinetics of Pu(IV) by hydroxyl-semicarbazide (HSC), hydroxyurea (HU) and di-hydroxyurea (DHU) in nitric acid solutions were investigated separately with adequate kinetic equations. In addition, counter-current cascade experiments were conducted for Pu split from U in nitric acid media using three kinds of reductant, respectively. The results show that urea derivatives as a kind of novel salt-free reductant can reduce Pu(IV) to Pu(III) rapidly in the nitric acid solutions. The stripping experimental results showed that Pu(IV) in the organic phase can be stripped rapidly to the aqueous phase by the urea derivatives, and the separation factors of plutonium /uranium can reach more than 10{sup 4}. This indicates that urea derivatives is a kind of promising salt-free agent for uranium/plutonium separation. In addition, the complexing effect of HSC with Np(IV) was revealed, and Np(IV) can be back-extracted by HSC with a separation factor of about 20.

  14. Natural fats and oils route to fatty alcohols

    SciTech Connect (OSTI)

    Buchold, H.

    1983-02-21

    The Philippines will be the site for a chemical plant that will use coconut oil to make fatty alcohols by means of a direct hydrogenation route - eliminating an intermediate esterification step. The facility will produce about 65,000 tonnes/yr of coconut fatty acid and 7,900 tonnes/yr of glycerine. About half of the fatty acid produced will be used to make 29,000 tonnes/yr of fatty alcohols. The principles and operation of the process are outlined.

  15. Effects of the addition of water-filled bladders or volatile fatty acids to the rumen on the feeding behaviour in lactating dairy cows

    E-Print Network [OSTI]

    Boyer, Edmond

    tests (rumen bladders and VFA infusion) were carried out at 4, 9, 15 and 35 wk after calving in 4) was compared to a bladder-free control. In the second test, a con- tinuous infusion of 20.3 MJ/d volatile fatty- men was compared to a control infusion (7 g NaCl, 3.7 g KCI and 2.4 ml HCI 2 N perI water

  16. Nucleic Acid Encoding A Lectin-Derived Progenitor Cell Preservation Factor

    DOE Patents [OSTI]

    Colucci, M. Gabriella (Dugenta, IT); Chrispeels, Maarten J. (La Jolla, CA); Moore, Jeffrey G. (New York, NY)

    2001-10-30

    The invention relates to an isolated nucleic acid molecule that encodes a protein that is effective to preserve progenitor cells, such as hematopoietic progenitor cells. The nucleic acid comprises a sequence defined by SEQ ID NO:1, a homolog thereof, or a fragment thereof. The encoded protein has an amino acid sequence that comprises a sequence defined by SEQ ID NO:2, a homolog thereof, or a fragment thereof that contains an amino acid sequence TNNVLQVT. Methods of using the encoded protein for preserving progenitor cells in vitro, ex vivo, and in vivo are also described. The invention, therefore, include methods such as myeloablation therapies for cancer treatment wherein myeloid reconstitution is facilitated by means of the specified protein. Other therapeutic utilities are also enabled through the invention, for example, expanding progenitor cell populations ex vivo to increase chances of engraftation, improving conditions for transporting and storing progenitor cells, and facilitating gene therapy to treat and cure a broad range of life-threatening hematologic diseases.

  17. Omega-3 fatty acid rich eggs as an alternative to fish in the diet: effects on human platelet aggregation and plasma lipids 

    E-Print Network [OSTI]

    Mayo, Patricia

    1996-01-01

    ) or menhaden oil (DHA, high in docosahexaenoic acid). Subjects consumed all types for 6 weeks with a 4 week washout period between each for a total of 3 test periods. Plasma total cholesterol (TC) and triacylglycerol (TG) were determined enzymatically...

  18. EFFECT OF HISTAMINE ON FREE FATTY ACIDS IN RUMINANTS' A. R. 0., The Vo%ani Center, P. 0. B. 6, Bet Dagan, Israel

    E-Print Network [OSTI]

    Boyer, Edmond

    deals with the effect of omasal or intravenous infusions of hista- mine on the level of plasma free.5g histamine-2HCI and 16 ml formic acid were infused into the oma- sum by means of a cannula inserted assay of FFA. In all experi- ments blood was collected immediately before the histamine infusions

  19. Structural determination of triclosan derivatives as inhibitors of Plasmodium falciparum enoyl reductase (PfENR) 

    E-Print Network [OSTI]

    Lucumi Moreno, Edinson

    2007-04-25

    -malaria drugs is necessary in modern days. Recent studies have shown that the parasite synthesizes fatty acids using a fatty acid synthase type II (FAS-II) instead of a type-I fatty acid synthase (FAS-I) that is present in other eukaryotes. Plasmodium falciparum...

  20. Low-Emissions Burner Technology using Biomass-Derived Liquid Fuels

    SciTech Connect (OSTI)

    2010-07-01

    The University of Alabama will develop fuel-flexible, low-emissions burner technology for the metal processing industry that is capable of using biomass-derived liquid fuels, such as glycerin or fatty acids, as a substitute for natural gas. By replacing a fossil fuel with biomass fuels, this new burner will enable a reduction in energy consumption and greenhouse gas emissions and an increase in fuel flexibility.

  1. Identification of 6-octadecynoic acid from a methanol extract of Marrubium vulgare L. as a peroxisome proliferator-activated receptor ? agonist

    SciTech Connect (OSTI)

    Ohtera, Anna; Miyamae, Yusaku; Nakai, Naomi [Graduate School of Biostudies, Kyoto University, Kyoto 606-8502 (Japan)] [Graduate School of Biostudies, Kyoto University, Kyoto 606-8502 (Japan); Kawachi, Atsushi; Kawada, Kiyokazu; Han, Junkyu; Isoda, Hiroko [Alliance for Research on North Africa (ARENA), University of Tsukuba, Ibaraki 305-8572 (Japan) [Alliance for Research on North Africa (ARENA), University of Tsukuba, Ibaraki 305-8572 (Japan); Faculty of Life and Environment, University of Tsukuba, Ibaraki 305-8572 (Japan); Neffati, Mohamed [Arid Zone Research Institute (IRA), Médenine 4119 (Tunisia)] [Arid Zone Research Institute (IRA), Médenine 4119 (Tunisia); Akita, Toru; Maejima, Kazuhiro [Nippon Shinyaku CO., LTD., Kyoto 601-8550 (Japan)] [Nippon Shinyaku CO., LTD., Kyoto 601-8550 (Japan); Masuda, Seiji; Kambe, Taiho [Graduate School of Biostudies, Kyoto University, Kyoto 606-8502 (Japan)] [Graduate School of Biostudies, Kyoto University, Kyoto 606-8502 (Japan); Mori, Naoki; Irie, Kazuhiro [Graduate School of Agriculture, Kyoto University, Kyoto 606-8502 (Japan)] [Graduate School of Agriculture, Kyoto University, Kyoto 606-8502 (Japan); Nagao, Masaya, E-mail: mnagao@kais.kyoto-u.ac.jp [Graduate School of Biostudies, Kyoto University, Kyoto 606-8502 (Japan)] [Graduate School of Biostudies, Kyoto University, Kyoto 606-8502 (Japan)

    2013-10-18

    Highlights: •6-ODA, a rare fatty acid with a triple bond, was identified from Marrubium vulgare. •6-ODA was synthesized from petroselinic acid as a starting material. •6-ODA stimulated lipid accumulation in HSC-T6 and 3T3-L1 cells. •The first report of a fatty acid with a triple bond functioning as a PPAR? agonist. •This study sheds light on novel functions of a fatty acid with a triple bond. -- Abstract: 6-Octadecynoic acid (6-ODA), a fatty acid with a triple bond, was identified in the methanol extract of Marrubium vulgare L. as an agonist of peroxisome proliferator-activated receptor ? (PPAR?). Fibrogenesis caused by hepatic stellate cells is inhibited by PPAR? whose ligands are clinically used for the treatment of diabetes. Plant extracts of Marrubium vulgare L., were screened for activity to inhibit fibrosis in the hepatic stellate cell line HSC-T6 using Oil Red-O staining, which detects lipids that typically accumulate in quiescent hepatic stellate cells. A methanol extract with activity to stimulate accumulation of lipids was obtained. This extract was found to have PPAR? agonist activity using a luciferase reporter assay. After purification using several chromatographic methods, 6-ODA, a fatty acid with a triple bond, was identified as a candidate of PPAR? agonist. Synthesized 6-ODA and its derivative 9-octadecynoic acid (9-ODA), which both have a triple bond but in different positions, activated PPAR? in a luciferase reporter assay and increased lipid accumulation in 3T3-L1 adipocytes in a PPAR?-dependent manner. There is little information about the biological activity of fatty acids with a triple bond, and to our knowledge, this is the first report that 6-ODA and 9-ODA function as PPAR? agonists.

  2. Design of bioavailable derivatives of 12-(3-adamantan-1-yl-ureido)dodecanoic acid, a potent

    E-Print Network [OSTI]

    Hammock, Bruce D.

    in poor in vivo availability. We investigated the effect of derivatization of the acid functional group results in compounds that are easier to formulate in animal food and in triglycerides for gavage and other: Food a

  3. Storage lipid biosynthesis in microspore-derived Brassica napus embryos

    SciTech Connect (OSTI)

    Taylor, D.C.; Underhill, E.W.; Weber, N. ); Pomeroy, M.K. ); Edwards, L. )

    1989-04-01

    Erucic acid, a fatty acid which is confined to the neutral lipids in developing seed cotyledons or rape, was chosen as a marker to study triacylglycerol (TAG) biosynthesis in a Brassica napus L. cv Reston microspore-derived embryo culture system. Accumulation and changes in acyl composition of TAGs during embryogenesis strongly paralleled that observed during seed development. Homogenates of 29-day cultured embryos were examined for the ability to incorporate erucoyl moieties into storage lipids. In the presence of {sup 14}C erucoyl CoA and various acceptors, including glycerol-3-phosphate (G3P), {sup 14}C erucic acid was rapidly incorporated into the TAG fraction. However, in contrast to studies with {sup 14}C oleoyl CoA, there was no measurable radioactivity in any Kennedy Pathway intermediates or within membrane lipid components. Analysis of the radiolabelled TAG species suggested that erucoyl moieties were incorporated into the sn-3 position by a highly active diacylglyercol acyltransferase.

  4. Method for production of petroselinic acid and OMEGA12 hexadecanoic acid in transgenic plants

    DOE Patents [OSTI]

    Ohlrogge, J.B.; Cahoon, E.B.; Shanklin, J.; Somerville, C.R.

    1995-07-04

    The present invention relates to a process for producing lipids containing the fatty acid, petroselinic acid, in plants. The production of petroselinic acid is accomplished by genetically transforming plants which do not normally accumulate petroselinic acid with a gene for a {omega}12 desaturase from another species which does normally accumulate petroselinic acid. 19 figs.

  5. Method for production of petroselinic acid and OMEGA12 hexadecanoic acid in transgenic plants

    DOE Patents [OSTI]

    Ohlrogge, John B. (Okemos, MI); Cahoon, Edgar B. (Lansing, MI); Shanklin, John (Upton, NY); Somerville, Christopher R. (Okemos, MI)

    1995-01-01

    The present invention relates to a process for producing lipids containing the fatty acid petroselinic acid in plants. The production of petroselinic acid is accomplished by genetically transforming plants which do not normally accumulate petroselinic acid with a gene for a .omega.12 desaturase from another species which does normally accumulate petroselinic acid.

  6. Docosahexaenoic acid and butyrate synergistically modulate intracellular calcium compartmentalization to induce colonocyte apoptosis 

    E-Print Network [OSTI]

    Kolar, Satya Sree N.

    2009-05-15

    Docosahexaenoic acid (DHA, 22:6n-3) from fish oil, and butyrate, a short-chain fatty acid fiber-fermentation product, protect against colon tumorigenesis in part by coordinately inducing apoptosis. We have demonstrated ...

  7. Engineering of bacterial methyl ketone synthesis for biofuels

    E-Print Network [OSTI]

    Goh, Ee-Been

    2012-01-01

    ketone synthesis for biofuels Ee-Been Goh†† 1,3 , Edward E.microbes for use as biofuels, such as fatty acid ethylother fatty acid-derived biofuels, such as fatty acid ethyl

  8. Omega-3 Fatty Acid Supplementation – a Potential Treatment for Autism?

    E-Print Network [OSTI]

    Bui, Diem

    2009-01-01

    in adults with severe autism Study type: open label with a1. Rapin I, Tuchman RF. Autism: Definition, Neurobiology,et al. Positive Screening for Autism in Ex- preterm Infants:

  9. Marketability of omega-3 fatty acid enriched shell eggs 

    E-Print Network [OSTI]

    Marshall, Autumn Chester

    1994-01-01

    on oxidative stability and flavor of enriched eggs, eggs were collected weekly (week 0, 1, 2, 3, 4) for each of 3 replicates/diet, and all weeks analyzed within 24 hours of the week 0 collection. Effect of storage on flavor of eggs (weeks 0, 2, 4...

  10. Hepatic fatty acid and cholesterol metabolism in nephrotic syndrome

    E-Print Network [OSTI]

    Han, Seungyeup; Vaziri, Nosratola D; Gollapudi, Pavan; Kwok, Vincent; Moradi, Hamid

    2013-01-01

    In addition, the NS ani- mals had increased plasmain the liver tissue of nephrotic ani- mals. The underlying

  11. Original article Effect of dietary supplementation of fatty acids

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    to the turbot brood- stock diet resulted in a significant increase in egg diameter and oil globule diameter [26 -- Five isonitrogenous diets (33% crude protein) were fed to the brood female carp, Catla catla (weighing ponds. Diet-I (control) contained only basic ingredients like rice bran, ground- nut oil cake, roasted

  12. Production Of Hydroxylated Fatty Acids In Genetically Modified Plants

    DOE Patents [OSTI]

    Hall, David R. (Provo, UT); Fox, Joe (Provo, UT)

    2002-05-21

    An annular wire harness for use in drill pipe comprising two rings interconnected by one or more insulated conductors. The rings are positioned within annular grooves located within the tool joints and the conductors are fixed within grooves along the bore wall of the pipe. The rings may be recessed within annular grooves in order to permit refacing of the tool joint. The rings are provided with means for coupling a power and data signal from an adjacent pipe to the conductors in such a fashion that the signal may be transmitted along the drill pipe and along an entire drill string.

  13. Color and fatty acid profiles of Texas pecans 

    E-Print Network [OSTI]

    Ratcliff, James R.

    1989-01-01

    Desire. ble 1. 47b Cultivar 'b'/'a' Cheyenne 1. 79a t Shaw uee 1. 74 a 41. 94a t 36. 88cd 7. 86b 7 39. 79ab 33. 02ef iae 10. 02cd 17. 77at 9. 10d f 15. 60b 9. 98cd 16. 86a 11. 70b 17. 27a 10. 03cd 14. 54b Odom 1. 46b 35. 10de 10. 62c 15. 39... Desirable (70-10-69) Oklahoma X Mahan X western (70-9-33) Caddo X Sunmer (76-14-17) ebs/car 3. 36a 2. 58b 2. 55bc 2. 20cd 2. 03cde 2. 01cde 44. 09a 38. 75deC 43. 31ab 38. 69def 41. 06bcd 39. 38cde cas 6. 07h e+ 6. 31h 8. 14fg 7. 80g 8...

  14. MFR PAPER 1033 Research determin es fatty aCid

    E-Print Network [OSTI]

    ~1 Preparation of Esters \\leth) I e\\ler lIere preparuJ u\\lng the mClhod :r, "ere \\cparated an d Idenlilied u\\lng , Zook E J Powell B Hackley J Emerson J Brooker. and G M Knobl

  15. Synthesis of Commodity Chemicals from Renewable Fatty Acids

    E-Print Network [OSTI]

    Stoltz, Brian M.

    polymerization, drilling fluids, and precursors to plasticizers, lubricants and surfactants. As of 2013, global

  16. Omega-3 Fatty Acid Supplementation – a Potential Treatment for Autism?

    E-Print Network [OSTI]

    Bui, Diem

    2009-01-01

    placebo consisted of 7 g of coconut oil plus 7 mg of vitaminplacebo for reducing received 7 g of coconut oil symptoms of

  17. Enrichment of selected fatty acids in broiler tissues 

    E-Print Network [OSTI]

    Yau, Jia-Chyi

    1990-01-01

    , 1984. Official Methods of Analysis. 14 ed. Association of Official Analytical Chemists, Washington, D. C. Baggio G. , A. Pagnan, M. Muraca, S. Martini, A. Opportuno, A. Bonanome, G. B. Ambrosio, S. Ferrari, P. Guarini, D. Piccolo, E. Mankato, R...

  18. A First Look at Yeast Fatty Acid Synthase

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of budding yeast that has been known since ancient times, and used in baking bread and brewing beer. It is also one of our distant relatives. Like animals and plants, budding yeast...

  19. Modification of the fatty acid composition of bovine tissues 

    E-Print Network [OSTI]

    Chang, Joyce

    1990-01-01

    of the As desaturase in rats is environmental temperatures, and the effect of cold temperatures on activity levels of these enzymes has been described (28, 29). These studies demonstrated that the desaturase system is sensitive to a number of external conditions... Crude protein Total lipids Crude fiber NE~ (McaUkg) NE& (Meal/kg) Calcium Phosphorus 89. 5 12. 2 9. 7 1. 74 1. 06 0. 41 0. 37 90. 2 12. 1 10. 4 9. 7 1. 86 1. 20 0. 42 0. 38 a Calculated 15 procedure first described by Morrison...

  20. A First Look at Yeast Fatty Acid Synthase

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach HomeA Better Anode Design to Improve Lithium-Ion Batteries Print Lithium-ionA First Look at Yeast

  1. Mutant Fatty Acid Desaturase and Method for Directed Mutagenesis - Energy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration wouldMass map shines light on77 PAGEMissionStressMoveMuncrief Ames LaboratoryInnovation

  2. Mutant Fatty Acid Desaturase and Method for Directed Mutagenesis - Energy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration wouldMass map shines light on77 PAGEMissionStressMoveMuncrief Ames

  3. A First Look at Yeast Fatty Acid Synthase

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of NaturalDukeWakefieldSulfateSciTechtail.TheoryTuesday, August 10, 20102016 News Below are4BElectron---neutrinoAA CleanAAAAA

  4. Fatty Acid Biosynthesis Caught in the Act | Stanford Synchrotron Radiation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration would like submitKansasCommunitiesofExtransScientific Userflow phenomena in a

  5. Structure–activity relationships of imidazole-derived 2-[N-carbamoylmethyl-alkylamino]acetic acids, dual binders of human insulin-degrading enzyme

    SciTech Connect (OSTI)

    Charton, Julie [Biostructures and Drug Discovery, Lille (France); Institut Pasteur de Lille, Lille (France); PRIM, Lille (France); CDithem Platform/IGM, Paris (France); Gauriot, Marion [Biostructures and Drug Discovery, Lille (France); Institut Pasteur de Lille, Lille (France); PRIM, Lille (France); CDithem Platform/IGM, Paris (France); Totobenazara, Jane [Biostructures and Drug Discovery, Lille (France); Institut Pasteur de Lille, Lille (France); PRIM, Lille (France); CDithem Platform/IGM, Paris (France); Hennuyer, Nathalie [Institut Pasteur de Lille, Lille (France); INSERM U1011 Nuclear Receptors, Lille (France); Dumont, Julie [Biostructures and Drug Discovery, Lille (France); Institut Pasteur de Lille, Lille (France); PRIM, Lille (France); CDithem Platform/IGM, Paris (France); Bosc, Damien [Biostructures and Drug Discovery, Lille (France); Institut Pasteur de Lille, Lille (France); PRIM, Lille (France); CDithem Platform/IGM, Paris (France); Marechal, Xavier [Biostructures and Drug Discovery, Lille (France); Institut Pasteur de Lille, Lille (France); PRIM, Lille (France); CDithem Platform/IGM, Paris (France); Elbakali, Jamal [Biostructures and Drug Discovery, Lille (France); Institut Pasteur de Lille, Lille (France); PRIM, Lille (France); CDithem Platform/IGM, Paris (France); Herledan, Adrien [Biostructures and Drug Discovery, Lille (France); Institut Pasteur de Lille, Lille (France); PRIM, Lille (France); CDithem Platform/IGM, Paris (France); Wen, Xiaoan [Biostructures and Drug Discovery, Lille (France); Institut Pasteur de Lille, Lille (France); PRIM, Lille (France); CDithem Platform/IGM, Paris (France); Ronco, Cyril [Biostructures and Drug Discovery, Lille (France); Institut Pasteur de Lille, Lille (France); PRIM, Lille (France); CDithem Platform/IGM, Paris (France); Gras-Masse, Helene [Biostructures and Drug Discovery, Lille (France); Institut Pasteur de Lille, Lille (France); PRIM, Lille (France); CDithem Platform/IGM, Paris (France); Heninot, Antoine [Biostructures and Drug Discovery, Lille (France); Institut Pasteur de Lille, Lille (France); PRIM, Lille (France); CDithem Platform/IGM, Paris (France); Pottiez, Virginie [Biostructures and Drug Discovery, Lille (France); Institut Pasteur de Lille, Lille (France); PRIM, Lille (France); CDithem Platform/IGM, Paris (France); Landry, Valerie [Biostructures and Drug Discovery, Lille (France); Institut Pasteur de Lille, Lille (France); PRIM, Lille (France); CDithem Platform/IGM, Paris (France); Staels, Bart [Institut Pasteur de Lille, Lille (France); INSERM U1011 Nuclear Receptors, Lille (France); Liang, Wenguang G. [The Univ. of Chicago, Chicago, IL (United States); Leroux, Florence [Biostructures and Drug Discovery, Lille (France); Institut Pasteur de Lille, Lille (France); PRIM, Lille (France); CDithem Platform/IGM, Paris (France); Tang, Wei -Jen [The Univ. of Chicago, Chicago, IL (United States); Deprez, Benoit [Biostructures and Drug Discovery, Lille (France); Institut Pasteur de Lille, Lille (France); PRIM, Lille (France); CDithem Platform/IGM, Paris (France); Deprez-Poulain, Rebecca [Biostructures and Drug Discovery, Lille (France); Institut Pasteur de Lille, Lille (France); PRIM, Lille (France); CDithem Platform/IGM, Paris (France)

    2015-01-01

    Insulin degrading enzyme (IDE) is a zinc metalloprotease that degrades small amyloid peptides such as amyloid-â and insulin. So far the dearth of IDE-specific pharmacological inhibitors impacts the understanding of its role in the physiopathology of Alzheimer's disease, amyloid-â clearance, and its validation as a potential therapeutic target. Hit 1 was previously discovered by high-throughput screening. Here we describe the structure-activity study, that required the synthesis of 48 analogues. We found that while the carboxylic acid, the imidazole and the tertiary amine were critical for activity, the methyl ester was successfully optimized to an amide or a 1,2,4-oxadiazole. Along with improving their activity, compounds were optimized for solubility, lipophilicity and stability in plasma and microsomes. The docking or co-crystallization of some compounds at the exosite or the catalytic site of IDE provided the structural basis for IDE inhibition. The pharmacokinetic properties of best compounds 44 and 46 were measured in vivo. As a result, 44 (BDM43079) and its methyl ester precursor 48 (BDM43124) are useful chemical probes for the exploration of IDE's role.

  6. Docosahexaenoic acid differentially modulates plasma membrane targeting and subcellular localization of lipidated proteins in colonocytes 

    E-Print Network [OSTI]

    Seo, Jeongmin

    2006-04-12

    Correct localization of lipidated cytosolic proteins to the plasma membrane (PM) is mediated by interactions between lipid anchors of proteins and cell membranes. Previously, dietary fish oil and its major n-3 polyunsaturated fatty acid (PUFA...

  7. Examining the effect of sterol carrier protein-2 on phytanic acid toxicity 

    E-Print Network [OSTI]

    Chen, Cynthia

    2013-02-22

    Although peroxisomes are essential organelles involved in the conversion of hydrogen peroxide to water, they also contain unique metabolic pathways in lipid metabolism. One of these is the "-oxidation of branched-chain fatty acids such as phytanic...

  8. Effect of different amounts and types of fat on colonic cell proliferation and fecal bile acid concentrations 

    E-Print Network [OSTI]

    Chang, Wen-Chi

    1992-01-01

    of the experiment, butter, soft margarine, hard margarine, and combination (1/3 butter, I/3 soft margarine, I/3 hard margarine) were used to supply different types of fat at 20% by weight. Table 4 shows the fatty acid composition for each different type of fat... minerals 1. 53 1. 53 1. 53 0. 51 0. 51 0. 51 choline 0, 10 0. 10 0. 10 TABLE 4. Fatty acid composition of the experimental diets by percentage Fatty acid butter soft mar arine hard mar arine combination 10:0 6. 06 2. 43 2. 82 12:0 5. 85 17. 14 7...

  9. Synthesis of labeled oxalic acid derivatives

    DOE Patents [OSTI]

    Martinez, Rodolfo A. (Santa Fe, NM); Unkefer, Clifford J. (Los Alamos, NM); Alvarez, Marc A. (Santa Fe, NM)

    2004-06-22

    The present invention is directed to labeled compounds, specifically ##STR1## where each C* is selected from the group consisting of a carbon-12, i.e., .sup.12 C, or a carbon-13, i.e., .sup.13 C and at least one C* is .sup.13 C, R.sup.1 is selected from the group of C.sub.1 -C.sub.4 lower alkyl and aryl, and X is selected from the group of --NR.sup.2 R.sup.3 where R.sup.2 and R.sup.3 are each independently selected from the group of C.sub.1 -C.sub.4 lower alkyl, alkoxy and aryl, --SR.sup.4 where R.sup.4 is selected from the group of C.sub.1 -C.sub.4 lower alkyl, alkoxy and aryl, and --OR.sup.5 where R.sup.5 is selected from the group of C.sub.1 -C.sub.4 lower alkyl, alkoxy and aryl with the proviso that when R.sup.1 is methyl then R.sup.5 is other than methyl, when R.sup.1 is ethyl then R.sup.5 is other than ethyl, and when R.sup.1 is benzyl then R.sup.5 is other than benzyl.

  10. Method for high specific bioproductivity of .alpha.,.omega.-alkanedicarboxylic acids

    DOE Patents [OSTI]

    Mobley, David Paul (Schenectady, NY); Shank, Gary Keith (Rocky Hill, CT)

    2000-01-01

    This invention provides a low-cost method of producing .alpha.,.omega.-alkanedicarboxylic acids. Particular bioconversion conditions result in highly efficient conversion of fatty acid, fatty acid ester, or alkane substrates to diacids. Candida tropicalis AR40 or similar yeast strains are grown in a medium containing a carbon source and a nitrogen source at a temperature of 31.degree. C. to 38.degree. C., while additional carbon source is continuously added, until maximum cell growth is attained. Within 0-3 hours of this point, substrate is added to the culture to initiate conversion. An .alpha.,.omega.-alkanedicarboxylic acid made according to this method is also provided.

  11. Lipid Metabolism, Gene Expression, Substrate Oxidation, and Meat Quality of Growing-finishing Pigs Supplemented with Conjugated Linoleic Acid and Arginine 

    E-Print Network [OSTI]

    Go, Gwang-Woong, 1979-

    2012-02-14

    .............................................. 9 Lipogenesis and CO2 production in vitro ...................................................... 10 Fatty acid composition .................................................................................. 11 Plasma amino acids... REVIEW OF LITERATURE General aspects of CLA and arginine. CLA is a group of geometric and positional isomers of linoleic acid, having conjugated double bonds (1), originating from biohydrogenation and isomerization of linoleic acid by rumen bacteria...

  12. Fatty Acid Engineering 2011 p. 1 Lipid signals: jasmonic acid & green leaf volatiles

    E-Print Network [OSTI]

    Constabel, Peter

    occurrence of modified 'specialty' FAs - genetic transformation technology available (NB: oil seeds amenable -> plastics - special 6 desaturase related to 9 stearoyl- ACP (18:0) desaturase. (NB: takes 16:0 ACP) - single

  13. Derived Types What Are Derived Types?

    E-Print Network [OSTI]

    Collett Jr., Jeffrey L.

    Derived Types #12;What Are Derived Types? As usual, a hybrid of two, unrelated concepts C++, Python orientation comes in #12;Simple Derived Types TYPE Wheel INTEGER :: spokes REAL :: diameter, width CHARACTER(LEN=15) :: material END TYPE Wheel That defines a derived type Wheel Using derived types needs a special

  14. Derived Types What Are Derived Types?

    E-Print Network [OSTI]

    Collett Jr., Jeffrey L.

    Derived Types #12;What Are Derived Types? As usual, a hybrid of two, unrelated concepts C object orientation comes in This course will only describe the former. #12;Simple Derived Types TYPE That defines a derived type Wheel Using derived types needs a special syntax TYPE(Wheel) :: w1 #12;More

  15. Factors determining the progression of nonalcoholic fatty liver disease; the role of abnormal fatty acid and glucocorticoid metabolism 

    E-Print Network [OSTI]

    MacFarlane, David Peter

    2011-11-25

    Obesity and insulin resistance are associated with a constellation of features including hypertension, dyslipidaemia, type 2 diabetes, and premature cardiovascular disease, collectively termed the metabolic syndrome. ...

  16. N-3 FATTY ACIDS AND JUVENILE TRAUMATIC BRAIN INJURY: EFFECTS OF DIETARY N-3 FATTY ACID CONTENT, N-3 FATTY ACID STATUS, AND ORALLY DOSED FISH OIL ON SENSORIMOTOR AND BIOCHEMICAL OUTCOMES

    E-Print Network [OSTI]

    Russell, Kristin L.

    2013-08-31

    Children under five years of age are at high risk for sustaining traumatic brain injury (TBI) and tend to have poorer outcomes despite greater neuroplasticity in children. Hence, there is a great need to study TBI specifically ...

  17. I. A1,3-Strain Enabled Retention of Chirality During Bis-Cyclization of ?-Ketoamides: Asymmetric Synthesis and Bioactivity of Salinosporamide A and Derivatives II. Optimization of an Organic Syntheses: Asymmetric Nucleophile-Catalyzed Aldol- Lactonization of Aldehyde Acids 

    E-Print Network [OSTI]

    Nguyen, Henry

    2010-11-17

    .2.4. Hatakeyama ? s Enantiosel e c t i v e Sy nthe sis of (?)-1.1a Hatakeyama?s synthesis of ( ? )-1.1a 6h began with propargyl alcohol 1.37 by a palladium mediated addition to an acetal dehyde derivative to give ether 1.38 . The PMB protection group...

  18. Topical vitamin-A-acid therapy for cutaneous metastatic melanoma.

    E-Print Network [OSTI]

    Levine, N; Meyskens, F L

    1980-01-01

    21: 236-7. 2. Bollag W, Ott F. Vitamin A acid in benign andZesch A. Penetration of vitamin A acid into human skin. Actaphosphoryl derivatives of vitamin A in biological membranes.

  19. Long range transport of acid rain precursors

    E-Print Network [OSTI]

    Fay, James A.

    1983-01-01

    A model of the long range transport of primary and secondary pollutants derived by Fay and Rosenzweig (1) is applied to the problem of the transport of acid rain precursors. The model describes the long term average (annual ...

  20. DERIVED FUNCTORS GROTHENDIECK DUALITY

    E-Print Network [OSTI]

    Lipman, Joseph

    NOTES ON DERIVED FUNCTORS AND GROTHENDIECK DUALITY Joseph Lipman In Foundations of Grothendieck the basics of derived categories and functors, and of the rich formalism, over ringed spaces, of the derived of the derived functor Rf when f is a quasi-proper map of concentrated schemes, the twisted inverse image

  1. The role of docosahexaenoic acid in mediating mitochondrial membrane lipid peroxidation and apoptosis in colonocytes. 

    E-Print Network [OSTI]

    Ng, Yee Voon

    2005-11-01

    ) or no fatty acid (control) for 72 h with or without 5 mM butyrate for the final 6-24 h. Real time analysis of cellular membrane lipid oxidation, as indicated by oxidation of a lipophilic vital dye, mitochondrial permeability transition (MPT), as characterized...

  2. Arginine and Conjugated Linoleic Acid Reduce Fat Mass in Rats 

    E-Print Network [OSTI]

    Nall, Jennifer L.

    2010-10-12

    = iv 0.09) palmitic acid incorporation into total lipids in epididymal adipose tissue. CLA plus arginine supplementation also reduced glucose incorporation into total lipid (P = 0.20). Arginine stimulated lipid synthesis from glucose in the absence... in the liver and decreases PPAR?2 expression in adipose tissue (18,19,20). PPAR?2 is a master gene that is found in brown and white adipose tissue. It acts to increase SCD expression and increase fatty acid synthesis (18,19,20). By decreasing PPAR...

  3. Carbonic Acid Pretreatment of Biomass

    SciTech Connect (OSTI)

    G. Peter van Walsum; Kemantha Jayawardhana; Damon Yourchisin; Robert McWilliams; Vanessa Castleberry

    2003-05-31

    This project sought to address six objectives, outlined below. The objectives were met through the completion of ten tasks. 1) Solidify the theoretical understanding of the binary CO2/H2O system at reaction temperatures and pressures. The thermodynamics of pH prediction have been improved to include a more rigorous treatment of non-ideal gas phases. However it was found that experimental attempts to confirm theoretical pH predictions were still off by a factor of about 1.8 pH units. Arrhenius experiments were carried out and the activation energy for carbonic acid appears to be substantially similar to sulfuric acid. Titration experiments have not yet confirmed or quantified the buffering or acid suppression effects of carbonic acid on biomass. 2) Modify the carbonic acid pretreatment severity function to include the effect of endogenous acid formation and carbonate buffering, if necessary. It was found that the existing severity functions serve adequately to account for endogenous acid production and carbonate effects. 3) Quantify the production of soluble carbohydrates at different reaction conditions and severity. Results show that carbonic acid has little effect on increasing soluble carbohydrate concentrations for pretreated aspen wood, compared to pretreatment with water alone. This appears to be connected to the release of endogenous acids by the substrate. A less acidic substrate such as corn stover would derive benefit from the use of carbonic acid. 4) Quantify the production of microbial inhibitors at selected reaction conditions and severity. It was found that the release of inhibitors was correlated to reaction severity and that carbonic acid did not appear to increase or decrease inhibition compared to pretreatment with water alone. 5) Assess the reactivity to enzymatic hydrolysis of material pretreated at selected reaction conditions and severity. Enzymatic hydrolysis rates increased with severity, but no advantage was detected for the use of carbonic acid compared to water alone. 6) Determine optimal conditions for carbonic acid pretreatment of aspen wood. Optimal severities appeared to be in the mid range tested. ASPEN-Plus modeling and economic analysis of the process indicate that the process could be cost competitive with sulfuric acid if the concentration of solids in the pretreatment is maintained very high (~50%). Lower solids concentrations result in larger reactors that become expensive to construct for high pressure applications.

  4. Biological Production of a Hydrocarbon Fuel Intermediate Polyhydroxybutyrate (PHB) from a Process Relevant Lignocellulosic Derived Sugar (Poster)

    SciTech Connect (OSTI)

    Wang, W.; Mittal, A.; Mohagheghi, A.; Johnson, D. K.

    2014-04-01

    PHAs are synthesized by many microorganisms to serve as intracellular carbon storage molecules. In some bacterial strains, PHB can account for up to 80% of cell mass. In addition to its application in the packaging sector, PHB also has great potential as an intermediate in the production of hydrocarbon fuels. PHB can be thermally depolymerized and decarboxylated to propene which can be upgraded to hydrocarbon fuels via commercial oligomerization technologies. Cupriavidus necator is the microorganism that has been most extensively studied and used for PHB production on an industrial scale; However the substrates used for producing PHB are mainly fructose, glucose, sucrose, fatty acids, glycerol, etc., which are expensive. In this study, we demonstrate production of PHB from a process relevant lignocellulosic derived sugar stream, i.e., saccharified slurry from pretreated corn stover. The strain was first investigated in shake flasks for its ability to utilize glucose, xylose and acetate. In addition, the strain was also grown on pretreated lignocellulose hydrolyzate slurry and evaluated in terms of cell growth, sugar utilization, PHB accumulation, etc. The mechanism of inhibition in the toxic hydrolysate generated by the pretreatment and saccharification process of biomass, was also studied.

  5. Modular pathway engineering of microbial fatty acid metabolism for the synthesis of branched acids, alcohols, and alkanes

    E-Print Network [OSTI]

    Sheppard, Micah J. (Micah James)

    2014-01-01

    Historically, microbial platforms have been used to synthesize a variety of chemical products and potential biofuels. More recently, increasingly complex metabolic pathways have been engineered by using novel hosts, modifying ...

  6. Absolute Time Derivatives

    E-Print Network [OSTI]

    T. Matolcsi; P. Van

    2006-10-23

    A four dimensional treatment of nonrelativistic space-time gives a natural frame to deal with objective time derivatives. In this framework some well known objective time derivatives of continuum mechanics appear as Lie-derivatives. Their coordinatized forms depends on the tensorial properties of the relevant physical quantities. We calculate the particular forms of objective time derivatives for scalars, vectors, covectors and different second order tensors from the point of view of a rotating observer. The relation of substantial, material and objective time derivatives is treated.

  7. Facilitated Phospholipid Flip-Flop Using Synthetic Steroid-Derived Translocases

    E-Print Network [OSTI]

    Smith, Bradley D.

    Facilitated Phospholipid Flip-Flop Using Synthetic Steroid-Derived Translocases Timothy N. Lambert research.4 Previously, we have reported that tris(2-aminoethyl)amine (tren)-derived translocases can of significantly more active synthetic translocases derived from cholic acid.6 Cholate derivatives 1-5 were

  8. Hydroxycarboxylic acids and salts

    SciTech Connect (OSTI)

    Kiely, Donald E; Hash, Kirk R; Kramer-Presta, Kylie; Smith, Tyler N

    2015-02-24

    Compositions which inhibit corrosion and alter the physical properties of concrete (admixtures) are prepared from salt mixtures of hydroxycarboxylic acids, carboxylic acids, and nitric acid. The salt mixtures are prepared by neutralizing acid product mixtures from the oxidation of polyols using nitric acid and oxygen as the oxidizing agents. Nitric acid is removed from the hydroxycarboxylic acids by evaporation and diffusion dialysis.

  9. Carbohydrate Derived-Pseudo-Lignin Can Retard Cellulose Biological Conversion

    E-Print Network [OSTI]

    California at Riverside, University of

    ARTICLE Carbohydrate Derived-Pseudo-Lignin Can Retard Cellulose Biological Conversion Rajeev Kumar derived pseudo-lignin on cellulose conversion at the moderate to low enzyme loadings necessary for favorable economics, dilute acid pretreatment of Avicel cellulose alone and mixed with beechwood xylan

  10. Concentrating aqueous volatile fatty acid salt solutions using a tertiary amine mixture 

    E-Print Network [OSTI]

    Gaskin, David J

    1997-01-01

    Lee (1993) has shown that tertiary amines are able to hics. extract water from low-concentration calcium acetate and sodium acetate solutions. This thesis extends the previous work to include calcium propionate and butyrate. Amine extraction may...

  11. Associations of Fatty Acids in Cerebrospinal Fluid with Peripheral Glucose Concentrations and Energy Metabolism

    E-Print Network [OSTI]

    Jumpertz, Reiner; Guijarro, Ana; Pratley, Richard E.; Mason, Clinton C.; Piomelli, Daniele; Krakoff, Jonathan

    2012-01-01

    composition was measured by dual energy X-ray absorptiometrycomposition was measured by dual-energy X-ray absorptio-

  12.  COMPOUND-SPECIFIC RADIOCARBON ANALYSES OF PHOSPHOLIPID FATTY ACIDS AND n -ALKANES IN OCEAN SEDIMENTS

    E-Print Network [OSTI]

    2010-01-01

    G. 1980. Environmental chemistry; an interdisciplinaryTechniques in Environmental Chemistry. Pergamon Series on

  13. Compound-Specific Radiocarbon Analyses of Phospholipid Fatty Acids and n-Alkanes in Ocean Sediments

    E-Print Network [OSTI]

    2010-01-01

    G. 1980. Environmental chemistry; an interdisciplinaryTechniques in Environmental Chemistry. Pergamon Series on

  14. Investigating fatty acid biosynthesis within the algal chloroplast using Chlamydomonas reinhardtii as a model

    E-Print Network [OSTI]

    Blatti, Jillian L.

    2012-01-01

    for bioenergy (i.e. bio-H 2 , bioethanol, and biodiesel),and carbohydrates fermented to bioethanol or converted toincluding biohydrogen, bioethanol, biodiesel, and

  15. Constructing and engineering fatty acid metabolic pathways for the production of fuels and chemicals

    E-Print Network [OSTI]

    Steen, Eric James

    2010-01-01

    Russell, D. W. Mammalian wax biosynthesis. I. IdentificationACB1 5.3.5. Expressing the wax ester synthase, atfA 5.3.6.A. AAC45217 calcoaceticus atfA, wax ester synthase A. baylyi

  16. Studies on the isolation and properties of the highly unsaturated fatty acids from natural sources 

    E-Print Network [OSTI]

    Abu-Nasr, Ahmed Mostafa

    1953-01-01

    m * ^ - 8 ?62 g. urea______ Complex 1 El^cm ? 1 *9 Filtrate Complex 2 Cool to 5 C# Filtrate +50 g. urea Filtrate Cool to 0 C# Filtrate s f *46.7 1 cm +30 g. urea and cool to 0? C. Filtrate ? i Complex 3 Ei*cm " 2... r-J (T < X GO 00 U.

  17. Sequential fatty acid analysis of a peat core covering the last two millennia (Tritrivakely lake, Madagascar)

    E-Print Network [OSTI]

    Boyer, Edmond

    , Madagascar): Diagenesis appraisal and consequences for palaeoenvironmental reconstruction Jean-Robert Disnar

  18. Investigating fatty acid biosynthesis within the algal chloroplast using Chlamydomonas reinhardtii as a model

    E-Print Network [OSTI]

    Blatti, Jillian L.

    2012-01-01

    current renewable energy issues, and are exposed to futurerenewable transportation fuel such as biodiesel has the potential to dramatically impact the future energy

  19. Volatile fatty acid fermentation of lime-treated bagasse by rumen microorganisms 

    E-Print Network [OSTI]

    Lee, Chang-Ming

    1993-01-01

    raw substrate (or 0.82 g VFA/g dry ash-free substrate or 0.94 g VFA/g dry ash-free, lignin- free substrate). This is greater than yields previously reported in the literature using lignocellulosic substrates, and hence demonstrates the superiority...

  20. Investigating fatty acid biosynthesis within the algal chloroplast using Chlamydomonas reinhardtii as a model

    E-Print Network [OSTI]

    Blatti, Jillian L.

    2012-01-01

    organism for biohydrogen production [25]. The photosyntheticalgae for biohydrogen and biofuel production. Currentbiofuel production Biofuel Biodiesel Bioethanol Biohydrogen

  1. Suppression of Lipid Autoxidation by Small Amounts of Isotope-reinforced Polyunsaturated Fatty Acids

    E-Print Network [OSTI]

    Tsui, Hui Su

    2013-01-01

    ml borosilicate glass tubes with PTFE lined screw caps untilml borosilicate glass tubes with PTFE lined screw caps untilBorosilicate glass tube with a PTFE lined screw cap. Lipid

  2. Constructing and engineering fatty acid metabolic pathways for the production of fuels and chemicals

    E-Print Network [OSTI]

    Steen, Eric James

    2010-01-01

    was performed using the lithium acetate method 12 . Strainswas performed using the lithium acetate method 24 . Strains

  3. Investigating fatty acid biosynthesis within the algal chloroplast using Chlamydomonas reinhardtii as a model

    E-Print Network [OSTI]

    Blatti, Jillian L.

    2012-01-01

    biomass feedstocks and yields of biomass feedstocks a) Amount of land requiredOil yields of leading biomass feedstocks. (Adapted from the

  4. Influence of Fatty Alcohols and Acids on the Clarity and Biota of Impounded Reservoirs 

    E-Print Network [OSTI]

    Davis, W. B.; Reynolds, T. D.

    1969-01-01

    This project presents results on a laboratory and field investigation of some of the biological and physical effects as a result of the use of a 1:1 mixture of hexadecanol and octadecanol. Laboratory investigations were ...

  5. An investigation of the origin and metabolism of the polyunsaturated fatty acids found in aquatic organisms 

    E-Print Network [OSTI]

    Kelly, Peter B.

    1958-01-01

    MfDGIVi Eg IcD EOBM gVEx IcD gOVMI SODIiVp DqfDVOxDNI TMONa xTBBDIr 1TGVEMD siMD SODIMr oyPo 200 250 300 350 374WAVE LENGTH IN MILLIMICRONS A - The oil of F. heteroclitus on their natural diet. B - The oil of F. heteroclitus on the fat-free diet.... C - The oil of F. heteroclitus on the fresh-water diet. D - The oil of F. heteroclitus on the marine diet. bAmdRh P QcD MfDGIVi Eg IcD EOBM gVEx IcD gOVMI SODIiVp DqfDVOxDNI TMONa bTNSTBTM cDIDVEGBOITMr 1TGVEMD siMD SODIM9 oyCo EXT INC TIO N...

  6. Investigating fatty acid biosynthesis within the algal chloroplast using Chlamydomonas reinhardtii as a model

    E-Print Network [OSTI]

    Blatti, Jillian L.

    2012-01-01

    budget to bioethanol production. The work conducted duringbiofuel production Biofuel Biodiesel Bioethanol Biohydrogen

  7. ORIGINAL ARTICLE Fatty Acid Profile of Sunshine Bass: II. Profile Change Differs

    E-Print Network [OSTI]

    . saxatilis) raised on feeds containing fish oil or 50:50 blend of fish oil and coconut, grapeseed, linseed affecting growth performance of the livestock. We previously demonstrated canola, coconut, corn, grapeseed

  8. Plasma lipidomics reveal profound perturbation of glycerophospholipids, fatty acids, and sphingolipids in diet-induced hyperlipidemia

    E-Print Network [OSTI]

    Miao, H; Chen, H; Pei, S; Bai, X; Vaziri, ND; Zhao, Y-Y

    2015-01-01

    bilayer structure, energy storage, signal ARTICLE IN PRESSserve as cellular energy storage and play an important role

  9. Fatty acid elongation and Delta9 desaturation: effects of diet and genetic background in cattle 

    E-Print Network [OSTI]

    Page, Amy Maurine

    1995-01-01

    on these enzyme systems we conducted three experiments. 1) The influence of feed on lipogenesis and enzyme activity: Elongase and desaturase activity in liver and elongase and desaturase activity, cellularity, and lipogenesis in subcutaneous adipose tissue were...

  10. Constructing and engineering fatty acid metabolic pathways for the production of fuels and chemicals

    E-Print Network [OSTI]

    Steen, Eric James

    2010-01-01

    costs and benefits of biodiesel and ethanol biofuels. Proccosts and benefits of biodiesel and ethanol biofuels. Procproduction of this biodiesel from hemicellulose demonstrates

  11. Polyploid genome of Camelina sativa revealed by isolation of fatty acid synthesis genes

    E-Print Network [OSTI]

    2010-01-01

    oil as a feedstock for biodiesel production. Industrialthe properties of C. sativa biodiesel are already well6], and both seed oil and biodiesel from C. sativa were used

  12. Investigating fatty acid biosynthesis within the algal chloroplast using Chlamydomonas reinhardtii as a model

    E-Print Network [OSTI]

    Blatti, Jillian L.

    2012-01-01

    microalgae for sustainable biodiesel. (2012) Blatti, J.L. ,energy within pond scum: Biodiesel from algal lipids. J.energy within pond scum: biodiesel from algal lipids. ”

  13. Investigating fatty acid biosynthesis within the algal chloroplast using Chlamydomonas reinhardtii as a model

    E-Print Network [OSTI]

    Blatti, Jillian L.

    2012-01-01

    from microalgae. Enzymatic Conversion of Biomass for Fuelsmicroalgae are capable of accumulating large quantities of oil, this is often associated with stressors that compromise biomassMicroalgae naturally accumulate energy-dense metabolites such as lipids ( Figure 1-2 ), some species producing oil up to 60% of their biomass [

  14. Cytoprotective Effects of Dietary Fatty Acids on Oxidative Stress in Hepatocytes 

    E-Print Network [OSTI]

    Richardson, Thomas C

    2012-07-11

    are most likely proteins that are susceptible to degradation upon carbonylation. Identification of these proteins by mass spectrometry will provide insights into the proteins susceptible to oxidative stress during steatosis, as well as serve as likely...

  15. Effects of dietary polyunsaturated fatty acid supplementation on urinary eicosanoids in canine chronic renal failure 

    E-Print Network [OSTI]

    Crocker, Raquel Cristina

    1996-01-01

    Changes in renal eicosanoids may be an index of disease progression or response to therapy in chronic renal failure (CRF). This study measured urinary thromboxane B2 (TXB2) and prostaglandin E2 (PGE2) concentrations in ...

  16. Anti-Oxidants, Omega-3 Fatty Acids, and Cognitive Decline with Aging

    E-Print Network [OSTI]

    Patel, Tanu Ashok

    2011-01-01

    neurobiology of cognitive aging”. Ageing Research Reviewsinterventions. Neurobiology of Aging , 133-136. Cutler, R.1991). Antioxidants and aging. American Journal of Clinical

  17. Volatile fatty acid fermentation of AFEX-treated newspaper and bagasse by rumen microorganisms 

    E-Print Network [OSTI]

    Blasig, Jorge Dari?o

    1991-01-01

    production vatied between 6. 6 and 8. 2 mmoles/g volatile solids (VS) digested and biogas (approximately 40% methane ranged from 0. 18 and 0. 29 L/g VS digested for organic waste materials of various origin and composition. The loading rates varied between...

  18. Investigating fatty acid biosynthesis within the algal chloroplast using Chlamydomonas reinhardtii as a model

    E-Print Network [OSTI]

    Blatti, Jillian L.

    2012-01-01

    as feedstocks for biofuel production: perspectives andas feedstocks for biofuel production: perspectives andof algae for enhanced biofuel production. Eukaryotic Cell 9:

  19. Preparation of Propylene Glycol Fatty Acid Ester or Other Glycol, or Polyol

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration wouldMass mapSpeedingProgram Guidelines This document w1.½tankSurfaceSciTechenergyand*

  20. Determination of Total Lipids as Fatty Acid Methyl Esters (FAME) by in situ

    Office of Scientific and Technical Information (OSTI)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of NaturalDukeWakefield MunicipalTechnical Report: Achievements ofCOMPOSITION OF VAPORSSeries)SupportedDavidA - L

  1. Fatty Acid-Producing Microbes for Generating Medium- and Long-Chain

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration would like submitKansasCommunitiesofExtransScientific Userflow phenomena in aHydrocarbons -

  2. Effets de drivs d'acides gras sur les ingestions volontaires d'aliments chez le mouton

    E-Print Network [OSTI]

    Boyer, Edmond

    Notre-Dame de la Paix, B-5000 Namur, Belgique. Summary. The continuous infusion of different fatty acid the same decreasing effect on the voluntary dry matter intake of adult sheep than the infusion of oleic jours au cours desquelles les sous-groupes reçoivent, en alternance, des infusions continues (1 1/animal

  3. Credit derivatives in Brazil

    E-Print Network [OSTI]

    Rüther, Henrique

    2007-01-01

    The amounts outstanding of credit derivatives have grown exponentially over the past years, and these financial intruments that allow market participants to trade credit risk have become very popular in Europe and in the ...

  4. Definitions Derived from Neutrosophics

    E-Print Network [OSTI]

    Florentin Smarandache

    2003-01-28

    Thirty-three new definitions are presented, derived from neutrosophic set, neutrosophic probability, neutrosophic statistics, and neutrosophic logic. Each one is independent, short, with references and cross references like in a dictionary style.

  5. Acid Violence in Pakistan

    E-Print Network [OSTI]

    Zia, Taiba

    2013-01-01

    Jordan. “Acid Attacks: Bangladesh’s Efforts to Stop thesubcontinent, especially in Bangladesh and Pakistan, and inon acid crimes in Bangladesh, for instance). Reliable data

  6. Production of Succinic Acid for Lignocellulosic Hydrolysates

    SciTech Connect (OSTI)

    Davison, B.H.; Nghiem, J.

    2002-06-01

    The purpose of this Cooperative Research and Development Agreement (CRADA) is to add and test new metabolic activities to existing microbial catalysts for the production of succinic acid from renewables. In particular, they seek to add to the existing organism the ability to utilize xylose efficiently and simultaneously with glucose in mixtures of sugars or to add succinic acid production to another strain and to test the value of this new capability for production of succinic acid from industrial lignocellulosic hydrolyasates. The Contractors and Participant are hereinafter jointly referred to as the 'Parties'. Research to date in succinic acid fermentation, separation and genetic engineering has resulted in a potentially economical process based on the use of an Escherichia coli strain AFP111 with suitable characteristics for the production of succinic acid from glucose. Economic analysis has shown that higher value commodity chemicals can be economically produced from succinic acid based on repliminary laboratory findings and predicted catalytic parameters. The initial target markets include succinic acid itself, succinate salts, esters and other derivatives for use as deicers, solvents and acidulants. The other commodity products from the succinic acid platform include 1,4-butanediol, {gamma}-butyrolactone, 2-pyrrolidinone and N-methyl pyrrolidinone. Current economic analyses indicate that this platform is competitive with existing petrochemical routes, especially for the succinic acid and derivatives. The report presents the planned CRADA objectives followed by the results. The results section has a combined biocatalysis and fermentation section and a commercialization section. This is a nonproprietary report; additional proprietary information may be made available subject to acceptance of the appropriate proprietary information agreements.

  7. Pushing schedule derivation method

    SciTech Connect (OSTI)

    Henriquez, B. [Compania Siderurgica Huachipato S.A., Talcahuano (Chile)

    1996-12-31

    The development of a Pushing Schedule Derivation Method has allowed the company to sustain the maximum production rate at CSH`s Coke Oven Battery, in spite of having single set oven machinery with a high failure index as well as a heat top tendency. The stated method provides for scheduled downtime of up to two hours for machinery maintenance purposes, periods of empty ovens for decarbonization and production loss recovery capability, while observing lower limits and uniformity of coking time.

  8. Asphaltene damage in matrix acidizing 

    E-Print Network [OSTI]

    Hinojosa, Roberto Antonio

    1996-01-01

    were acidized with three stage treatments of 15% hydrochloric acid (HCl), 12% HCL-3% hydrofluoric acid (HF) and 15% HCL. No additives were used in the acid. Comparisons were made between cores acidized with a variety of saturating fluids. Petrographic...

  9. Vaporization Enthalpy and Vapor Pressure of Valproic Acid by Correlation Gas Chromatography

    E-Print Network [OSTI]

    Chickos, James S.

    Vaporization Enthalpy and Vapor Pressure of Valproic Acid by Correlation Gas Chromatography Joe A-propylpentanoic acid) is reported, and the vapor pressures of a series on aliphatic carboxylic acids are used to evaluate its vapor pressure as a function of temperature. The vaporization enthalpy was derived

  10. Formic Acid Mechanical,

    E-Print Network [OSTI]

    Lee, Tonghun

    that make building block chemicals. Ethanol is blended with gasoline, reducing our dependence on petroleum for high valued products, including: Aspartic acid Glucaric acid Glycerol Sorbitol Some companies already

  11. Effect of Root-Derived Substrates on the Expression of nah-lux Genes in

    E-Print Network [OSTI]

    Alvarez, Pedro J.

    -derived substrates (e.g., sugars, carboxylic acids, amino acids, and phenolics) on the expression of nahG, one of the genes responsible for naphthalene dioxygenase transcription. Whereas nahG was induced by some phenolic degradation assays. However, treatments with root extracts exhibited significantly higher microbial growth

  12. Nucleic acid detection assays

    DOE Patents [OSTI]

    Prudent, James R.; Hall, Jeff G.; Lyamichev, Victor I.; Brow, Mary Ann; Dahlberg, James E.

    2005-04-05

    The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

  13. Nucleic acid detection compositions

    DOE Patents [OSTI]

    Prudent, James R. (Madison, WI); Hall, Jeff G. (Madison, WI); Lyamichev, Victor I. (Madison, WI); Brow, Mary Ann (Madison, WI); Dahlberg, James L. (Madison, WI)

    2008-08-05

    The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

  14. Cleavage of nucleic acids

    DOE Patents [OSTI]

    Prudent, James R. (Madison, WI); Hall, Jeff G. (Waunakee, WI); Lyamichev, Victor I. (Madison, WI); Brow; Mary Ann D. (Madison, WI); Dahlberg, James E. (Madison, WI)

    2010-11-09

    The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

  15. Cleavage of nucleic acids

    DOE Patents [OSTI]

    Prudent, James R. (Madison, WI); Hall, Jeff G. (Madison, WI); Lyamichev, Victor L. (Madison, WI); Brow, Mary Ann D. (Madison, WI); Dahlberg, James E. (Madison, WI)

    2007-12-11

    The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

  16. Metal reduction at low pH by a Desulfosporosinus species: implications for the biological1 treatment of acidic mine drainage2

    E-Print Network [OSTI]

    Burgos, William

    treatment of acidic mine drainage2 3 John M. Senko1,2 *, Gengxin Zhang2§ , Jeffrey T. McDonough4 , Mary Ann, GBSRB4.2, from coal mine-derived23 acidic mine drainage (AMD)-derived sediments. Sequence analysisH 7.1 groundwater.32 #12;3 INTRODUCTION33 Acidic mine drainage (AMD) arises when sulfide

  17. Nucleic acid detection kits

    DOE Patents [OSTI]

    Hall, Jeff G.; Lyamichev, Victor I.; Mast, Andrea L.; Brow, Mary Ann; Kwiatkowski, Robert W.; Vavra, Stephanie H.

    2005-03-29

    The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of nucleic acid from various viruses in a sample.

  18. Microorganisms for producing organic acids

    DOE Patents [OSTI]

    Pfleger, Brian Frederick; Begemann, Matthew Brett

    2014-09-30

    Organic acid-producing microorganisms and methods of using same. The organic acid-producing microorganisms comprise modifications that reduce or ablate AcsA activity or AcsA homolog activity. The modifications increase tolerance of the microorganisms to such organic acids as 3-hydroxypropionic acid, acrylic acid, propionic acid, lactic acid, and others. Further modifications to the microorganisms increase production of such organic acids as 3-hydroxypropionic acid, lactate, and others. Methods of producing such organic acids as 3-hydroxypropionic acid, lactate, and others with the modified microorganisms are provided. Methods of using acsA or homologs thereof as counter-selectable markers are also provided.

  19. Production of Chemical Derivatives from Renewables

    SciTech Connect (OSTI)

    Davison, Brian; Nghiem, John; Donnelly, Mark; Tsai, Shih-Perng; Frye, John; Landucci, Ron; Griffin, Michael

    1996-06-01

    The purpose of this Cooperative Research and Development Agreement (CRADA) between Lockheed Martin Energy Research Corp., (LMER), Argonne National Laboratory (ANL), National Renewable Energy Laboratory (NREL), and Battelle Memorial Institute, operator of Pacific Northwest National Laboratory (PNNL), (collectively referred to as the 'Contractor'), and Applied Carbochemicals, Inc. (Participant) was to scale-up from bench results an economically promising and competitive process for the production of chemical derivatives from biologically produced succinic acid. The products that were under consideration for production from the succinic acid platform included 1,4-butanediol, {gamma}y-butyrolactone, 2-pyrrolidinone and N-methyl pyrrolidinone. Preliminary economic analyses indicated that this platform was competitive with the most recent petrochemical routes. The Contractors and participant are hereinafter jointly referred to as the 'Parties.' Research to date in succinic acid fermentation, separation and genetic engineering resulted in a potentially economical process based on the use of an Escherichia coli strain AFP111 with suitable characteristics for the production of succinic acid from glucose. Economic analysis has shown that higher value commodity chemicals can be economically produced from succinic acid based on preliminary laboratory findings and predicted catalytic parameters. At the time, the current need was to provide the necessary laboratory follow-up information to properly optimize, design and operate a pilot scale process. The purpose of the pilot work was to validate the integrated process, assure 'robustness' of the process, define operating conditions, and provide samples for potential customer evaluation. The data from the pilot scale process was used in design and development of a full scale production facility. A new strain, AFP111 (patented), discovered at ANL was tested and developed for process use at the Oak Ridge National Laboratory (ORNL) and ANL. The operability and product formation are attractive for this strain and effort was being directed at process development and optimization. Key to the transition from the fermentative production unit operation to the chemical catalysis is the 'clean-up' of fermentation broth, succinic acid formation from the salt, and succinic acid concentration. These steps are accomplished by a two-stage membrane ED separation process developed at AWL. Although the current process is well developed, possible modifications and optimization may be called for as development work continues in both the fermentation and catalysis areas. Research to date performed at PNNL has demonstrated that succinic acid can be converted to value added chemicals such as 1,4-butanediol, {gamma}-butyrolactone, N-methyl pyrrolidinone, and 2 pyrrolidinone with high conversion and selectivities. Continued research will be performed in catalyst development and reaction condition optimization to move this work from the bench scale to the pilot scale. All development of the process was guided by the NREL technoeconomic model. The model showed that direct aqueous phase catalysis of succinic acid to 1,4-butanediol, {gamma}-butyrolactone, and N-methyl pyrrolidinone provided significant economical advantages in the market, the margin, and the return on capital investment over existing petrochemical processes for production of these compounds. The model also provided the baseline for evaluating current laboratory research. As data from the bench and pilot work were made available the model was modified and appropriate sensitivities ran to determine impact of the process changes and optimization. The report will present the planned CRADA tasks followed by the results. The results section has an overall project summary follwed by more detailed reports from the participants. This is a nonproprietary report; additional proprietary information may be made available subject to acceptance of the appropriate proprietary information agreements.

  20. Process Design and Economics for the Conversion of Lignocellulosic Biomass to Hydrocarbons: Dilute-Acid and Enzymatic Deconstruction of Biomass to Sugars and Biological Conversion of Sugars to Hydrocarbons

    SciTech Connect (OSTI)

    Davis, R.; Tao, L.; Tan, E. C. D.; Biddy, M. J.; Beckham, G. T.; Scarlata, C.; Jacobson, J.; Cafferty, K.; Ross, J.; Lukas, J.; Knorr, D.; Schoen, P.

    2013-10-01

    This report describes one potential conversion process to hydrocarbon products by way of biological conversion of lingnocellulosic-dervied sugars. The process design converts biomass to a hydrocarbon intermediate, a free fatty acid, using dilute-acid pretreatement, enzymatic saccharification, and bioconversion. Ancillary areas--feed handling, hydrolysate conditioning, product recovery and upgrading (hydrotreating) to a final blendstock material, wastewater treatment, lignin combusion, and utilities--are also included in the design.

  1. Recovery of organic acids

    DOE Patents [OSTI]

    Verser, Dan W. (Golden, CO); Eggeman, Timothy J. (Lakewood, CO)

    2009-10-13

    A method is disclosed for the recovery of an organic acid from a dilute salt solution in which the cation of the salt forms an insoluble carbonate salt. A tertiary amine and CO.sub.2 are introduced to the solution to form the insoluble carbonate salt and a complex between the acid and an amine. A water immiscible solvent, such as an alcohol, is added to extract the acid/amine complex from the dilute salt solution to a reaction phase. The reaction phase is continuously dried and a product between the acid and the solvent, such as an ester, is formed.

  2. Recovery of organic acids

    DOE Patents [OSTI]

    Verser, Dan W. (Menlo Park, CA); Eggeman, Timothy J. (Lakewood, CO)

    2011-11-01

    A method is disclosed for the recovery of an organic acid from a dilute salt solution in which the cation of the salt forms an insoluble carbonate salt. A tertiary amine and CO.sub.2 are introduced to the solution to form the insoluble carbonate salt and a complex between the acid and an amine. A water immiscible solvent, such as an alcohol, is added to extract the acid/amine complex from the dilute salt solution to a reaction phase. The reaction phase is continuously dried and a product between the acid and the solvent, such as an ester, is formed.

  3. Reversible Acid Gas Capture

    ScienceCinema (OSTI)

    Dave Heldebrant

    2012-12-31

    Pacific Northwest National Laboratory scientist David Heldebrant demonstrates how a new process called reversible acid gas capture works to pull carbon dioxide out of power plant emissions.

  4. HYDROFLUORIC ACID Safety Office

    E-Print Network [OSTI]

    Davis, Lloyd M.

    in industrial processes: glass etching, metal cleaning, laboratory reagents, etc. Can be found in household products: rust removers, automotive detailing products, stain removers. #12;Hydrofluoric Acid ­ Chemical

  5. Process for producing peracids from aliphatic hydroxy carboxylic acids

    DOE Patents [OSTI]

    Chum, H.L.; Palasz, P.D.; Ratcliff, M.A.

    1984-12-20

    A process is described for producing peracids from lactic acid-containing solutions derived from biomass processing systems. It consists of adjusting the pH of the solution to about 8 to 9 and removing alkaline residue fractions therefrom to form a solution comprised substantially of lower aliphatic hydroxy acids. The solution is oxidized to produce volatile lower aliphatic aldehydes. The aldehydes are removed as they are generated and converted to peracids.

  6. Materials and methods for efficient lactic acid production

    SciTech Connect (OSTI)

    Zhou, Shengde; Ingram, Lonnie O'Neal; Shanmugam, Keelnatham T; Yomano, Lorraine; Grabar, Tammy B; Moore, Jonathan C

    2013-04-23

    The present invention provides derivatives of Escherichia coli constructed for the production of lactic acid. The transformed E. coli of the invention are prepared by deleting the genes that encode competing pathways followed by a growth-based selection for mutants with improved performance. These transformed E. coli are useful for providing an increased supply of lactic acid for use in food and industrial applications.

  7. Materials and methods for efficient lactic acid production

    DOE Patents [OSTI]

    Zhou, Shengde (Sycamore, IL); Ingram, Lonnie O'Neal (Gainesville, FL); Shanmugam, Keelnatham T. (Gainesville, FL); Yomano, Lorraine (Gainesville, FL); Grabar, Tammy B. (Gainesville, FL); Moore, Jonathan C. (Gainesville, FL)

    2009-12-08

    The present invention provides derivatives of ethanologenic Escherichia coli K011 constructed for the production of lactic acid. The transformed E. coli of the invention are prepared by deleting the genes that encode competing pathways followed by a growth-based selection for mutants with improved performance. These transformed E. coli are useful for providing an increased supply of lactic acid for use in food and industrial applications.

  8. A determination of the heats of combustion of the five-carbon fatty acids and their methyl and ethyl esters 

    E-Print Network [OSTI]

    Gilby, Ralph F

    1952-01-01

    in the sehcsl dateasdmtdccw ef ths haste of oosbccstico ef tks sccLds scsL ef their ostace? In this wsy& ~ of the oonstsnt errorsi ssoh sec hest incest frcsc stirring? the hest fer elaotrio cnrrent ~sry te Tbs eaayls ef hsnseio scdd to bs cceed wss weighed...

  9. Milk fat composition of a lactating dairy cow as influenced by abomasal infusion of various long chain fatty acids 

    E-Print Network [OSTI]

    Switzer, Leonard Anderson

    1971-01-01

    Ql 0 tD E IQ CD 4 ID 3 0 0 0 4 Q? 0 ttl CQ 0 0 4 4 LQ 0 Ln CJ 4 tD 3 0 Q- Q? IQ cnl 4 tD 3 Ql 0 CI 0 0 Q- 4 L- ID 0 Gl Lnl o cn 0 4 CQ 3 tD 0 0 0 C Q- 4 Q- IQ 0 Ql cnl cl cn ID 37 IQ V tl In U 4 O cnl 0 CO F ll O... in the same column uith dif'ferent superscripts (a, b, c) differed significantly (P&0. 05). 20 03 C 'I Cll IO LO I CO M OI IO N M IO N IO H C7I 4 LC3 D N M C LO CO D rt W N t N D Ot Cll N N CO LO IO (O CO D D D N \\0 IO LO (8 N N N LO M D f71 CII...

  10. Effect of Omega 3 Polyunsaturated Fatty Acids (PUFAs) on Markers of Inflammation in Young Horses in Training 

    E-Print Network [OSTI]

    Lucia, Jessica Lauren

    2011-02-22

    was obtained from right radial carpal joint by arthrocentesis every 28 d and was analyzed for white blood cell count (WBC), total protein (TP), and specific gravity (SG). Serum concentrations of carboxypeptide type II collagen (CPII) and chondroitin sulfate 846...

  11. The response of fatty acids and pigments to variations in temperature and irradiance in the Marine Diatom Thalassiosira pseudonana :

    E-Print Network [OSTI]

    Shang, Frank F.

    2011-01-01

    in Temperature and Irradiance on the Diatom ThalassiosiraSizes of the 18 ? C, at 3 irradiance levels 80, 240 and 420Srezepek. Influence of irradiance and temperature on the

  12. Effect of dietary polyunsaturated fatty acid and related nutrients on plasma lipids, and skin and hair coat condition in canines 

    E-Print Network [OSTI]

    Hester, Shaleah Lynnae

    2004-11-15

    A study was performed to investigate the effect of diet modifications on skin and hair coat condition in canines. The study included 24 normal adult dogs fed a baseline diet (Ol'Roy[trademark]), during an acclimation period of 12 wk (Phase I). Nine...

  13. Effect of dietary polyunsaturated fatty acids and related nutrients on sebum lipids, and skin and hair coat condition in canines 

    E-Print Network [OSTI]

    Kirby, Naomi Anne

    2005-02-17

    feeding period of Phase II, test results revealed statistically significant increases in both CE and CE/WD, and a prolonged growth of hair follicles in the anagen phase of diet B dogs, which are both consistent with improved skin and hair coat scores....

  14. n-3 Polyunsaturated Fatty Acids Alter Mouse CD4+ T Cell Activation by Modifying the Lipid Bilayer Properties 

    E-Print Network [OSTI]

    Hou, Tim Yu-Tien

    2014-12-05

    phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2], which modulates actin remodeling, is perturbed by n-3 PUFA. Utilizing the transgenic Fat-1 mouse model that synthesizes n-3 PUFA de novo and enriches the plasma membrane with n-3 PUFA, and wild type (WT) mice fed...

  15. The effect of dietary n-3 polyunsaturated fatty acids on T cell subset activation-induced cell death 

    E-Print Network [OSTI]

    Switzer, Kirsten Collette

    2004-11-15

    (control) or n-3 PUFA for 14 d. Splenic T cells were stimulated with ?CD3/?CD28, ?CD3/PMA, or PMA/Ionomycin for 48 h followed by reactivation with the same stimuli for 5 h. Apoptosis was measured using Annexin V/propidium iodide and flow cytometry...

  16. Cryptosporidium parvum: enhancing our understanding of its unique fatty acid metabolism and the elucidation of putative new inhibitors 

    E-Print Network [OSTI]

    Fritzler, Jason Michael

    2008-10-10

    synthase (CpFAS1), C. parvum also possesses an enormous type I polyketide synthase (CpPKS1). Because of the size of this megasynthase, functional characterization of the complete enzyme is not possible. We have isolated and characterized the loading unit...

  17. The response of fatty acids and pigments to variations in temperature and irradiance in the Marine Diatom Thalassiosira pseudonana :

    E-Print Network [OSTI]

    Shang, Frank F.

    2011-01-01

    Oliveria Luisa Gouveia. Microalgae as a raw materal forunique promise. Microalgae are efficient photoautotrophicto combat global warming. Microalgae embody an exceptionally

  18. Organ-Specific Alterations in Fatty Acid De Novo Synthesis and Desaturation in a Rat Model of Programmed Obesity

    E-Print Network [OSTI]

    Yee, Jennifer K; Lee, Wai-Nang P; Han, Guang; Ross, Michael G; Desai, Mina

    2011-01-01

    end of the nursing period, then develop adult obesity. Thethe end of the nursing period, and become obese adults. The

  19. N-3 Polyunsaturated Fatty Acids Reduce Th17 Differentiation by Decreasing Responsiveness to Interleukin-6 in Mice 

    E-Print Network [OSTI]

    Allen, Marilyn Jean

    2014-05-29

    CD4^(+) effector T cell subsets (e.g., Th1, Th17) are implicated in autoimmune and inflammatory disorders such as multiple sclerosis, psoriasis and rheumatoid arthritis. For optimal activation, IL-6 induces Th17 polarization and signals through...

  20. n-3 Polyunsaturated Fatty Acids Suppress Mitochondrial Translocation to the Immunological Synapse and Modulate Calcium Signaling in T Cells 

    E-Print Network [OSTI]

    Yog, Rajeshwari

    2011-02-22

    T helper (Th) cell activation is necessary for the adaptive immune response. Formation of an immunological synapse (IS) between Th cells and antigen-presenting cells is the first step in Th cell activation. In vitro studies indicate that formation...

  1. Dietary supplementation of omega-3 fatty acids and subsequent effects on fresh, cooled, and frozen seminal characteristics of stallions 

    E-Print Network [OSTI]

    Grady, Sicilia Tatiana

    2009-05-15

    The use of cooled and frozen/thawed semen offers many advantages to breeders. However, many stallions produce spermatozoa that are unable to endure the stresses of cooling/storage and freezing/thawing. Improving the quality ...

  2. Development and validation of a robust automated analysis of plasma phospholipid fatty acids for metabolic phenotyping of large epidemiological studies

    E-Print Network [OSTI]

    Wang, Laura Y.; Summerhill, Keith; Rodriguez-Canas, Carmen; Mather, Ian; Patel, Pinal; Eiden, Michael; Young, Stephen; Forouhi, Nita G.; Koulman, Albert

    2013-04-25

    from the different fractions of human biological fluids is a laborious task. In previous projects, in which sample sets were analyzed using conventional manual methods, it was found that a team of two people could maintain a throughput of around 300... , and transfer to a clean vial by [2] from [4]. 10. Dry the crude extract under a stream of nitrogen at 55°C on [7]. 11. Reconstitute the crude extract in 2.0 ml dry chloroform by [2] from [4]. 12. Pass the crude extract through a 1 ml aminopropyl silica...

  3. Direct comparison of fatty acid ratios in single cellular lipid droplets as determined by comparative Raman spectroscopy and gas chromatography

    E-Print Network [OSTI]

    2013-01-01

    by AsLS, were indicated by pie charts, and expressed asnumerically below the pie charts. we show the correspondingusing AsLS ?tting. The pie chart indicates the relative

  4. The response of fatty acids and pigments to variations in temperature and irradiance in the Marine Diatom Thalassiosira pseudonana :

    E-Print Network [OSTI]

    Shang, Frank F.

    2011-01-01

    microalgae generate lipids, alcohols, hydrogen, and polysaccharides that can all be transformed into fuel [12]; the byproducts and waste biomass

  5. Controlling acid rain

    E-Print Network [OSTI]

    Fay, James A.

    1983-01-01

    High concentrations of sulfuric and nitric acid in raTn fn the northeastern USA are caused by the large scale combustion of fossil fuels within this region. Average precipitation acidity is pH 4.2, but spatial and temporal ...

  6. Processes for converting lignocellulosics to reduced acid pyrolysis oil

    DOE Patents [OSTI]

    Kocal, Joseph Anthony; Brandvold, Timothy A

    2015-01-06

    Processes for producing reduced acid lignocellulosic-derived pyrolysis oil are provided. In a process, lignocellulosic material is fed to a heating zone. A basic solid catalyst is delivered to the heating zone. The lignocellulosic material is pyrolyzed in the presence of the basic solid catalyst in the heating zone to create pyrolysis gases. The oxygen in the pyrolysis gases is catalytically converted to separable species in the heating zone. The pyrolysis gases are removed from the heating zone and are liquefied to form the reduced acid lignocellulosic-derived pyrolysis oil.

  7. Solubility of small-chain carboxylic acids in supercritical carbon dioxide

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Sparks, Darrell L.; Este?vez, L. Antonio; Hernandez, Rafael; McEwen, Jason; French, Todd

    2010-07-08

    The solubility of heptanoic acid and octanoic acid in supercritical carbon dioxide has been determined at temperatures of (313.15, 323.15, and 333.15) K over a pressure range of (8.5 to 30.0) MPa, depending upon the solute. The solubility of heptanoic acid ranged from a solute concentration of (0.08 ± 0.03) kg • m-3 (T = 323.15 K, p = 8.5 MPa) to (147 ± 0.2) kg • m-3 (T = 323.15 K, p = 20.0 MPa). The lowest octanoic acid solubility obtained was a solute concentration of (0.40 ± 0.1) kg • m-3 (T = 333.15 K, p = 10.0more »MPa), while the highest solute concentration was (151 ± 2) kg • m-3 (T = 333.15 K, p = 26.7 MPa). In addition, solubility experiments were performed for nonanoic acid in supercritical carbon dioxide at 323.15 K and pressures of (10.0 to 30.0) MPa to add to the solubility data previously published by the authors. In general, carboxylic acid solubility increased with increasing solvent density. The results also showed that the solubility of the solutes decreased with increasing molar mass at constant supercritical-fluid density. Additionally, the efficacy of Chrastil's equation and other density-based models was evaluated for each fatty acid.« less

  8. Long Chain Fatty Acyl-Coenzyme A Synthetase as Novel Drug Targets in Cryptosporidium parvum 

    E-Print Network [OSTI]

    Guo, Fengguang

    2014-07-07

    screening hundreds of compounds in vitro and in vivo, fully effective therapeutic agents are still unavailable. The major goal of this study is to explore three long-chain fatty acyl-CoA synthetase (CpACSs) of the parasite as novel drug targets...

  9. Higher-derivative Schwinger model

    SciTech Connect (OSTI)

    Amaral, R.L.P.G.; Belvedere, L.V.; Lemos, N.A. (Instituto de Fisica, Universidade Federal Fluminense, Outeiro de Sao Joao Batista s/n, 24020 Centro, Niteroi, Rio de Janeiro (Brazil)); Natividade, C.P. (Departamento de Matematica, Universidade Estadual Paulista, Campus de Guaratingueta, 12500 Sao Paulo, Sao Paulo (Brazil))

    1993-04-15

    Using the operator formalism, we obtain the bosonic representation for the free fermion field satisfying an equation of motion with higher-order derivatives. Then, we consider the operator solution of a generalized Schwinger model with higher-derivative coupling. Since the increasing of the derivative order implies the introduction of an equivalent number of extra fermionic degrees of freedom, the mass acquired by the gauge field is bigger than the one for the standard two-dimensional QED. An analysis of the problem from the functional integration point of view corroborates the findings of canonical quantization, and corrects certain results previously announced in the literature on the basis of Fujikawa's technique.

  10. SYNTHESIS OF METHACRYLATES FROM COAL-DERIVED SYNGAS

    SciTech Connect (OSTI)

    Jang, B.W.L.; Spivey, J.J.; Gogate, M.R.; Zoeller, J.R.; Colberg, R.D.; Choi, G.N.

    1999-12-01

    Research Triangle Institute (RTI), Eastman Chemical Company, and Bechtel have developed a novel process for synthesis of methyl methacrylate (MMA) from coal-derived syngas, under a contract from the US Department of Energy/Fossil Energy Technology Center (DOE/FETC). This project has resulted in five US patents (four already published and one pending publication). It has served as the basis for the technical and economic assessment of the production of this high-volume intermediate from coal-derived synthesis gas. The three-step process consists of the synthesis of a propionate from ethylene carbonylation using coal-derived CO, condensation of the propionate with formaldehyde to form methacrylic acid (MAA); and esterification of MAA with methanol to yield MMA. The first two steps, propionate synthesis and condensation catalysis, are the key technical challenges and the focus of the research presented here.

  11. Quercetin and Chlorogenic Acid Mitigate DSS-Induced Changes in Expression of Select Pro-Inflammatory Cytokines and Short Chain Fatty Acid Transporter Genes 

    E-Print Network [OSTI]

    Piefer, Leigh

    2012-10-19

    as many different types of phytochemicals. Phytochemicals are secondary metabolites synthesized by plants, and include chemical classes such phenols, anthocyanins, and flavonols. The composition of phytochemicals varies between not only the types... and vegetables are a few ways to reduce risk of colon cancer (2, 3). These food groups are nutrient-dense and a good source of fiber, phytochemicals, and antioxidants (3). Peaches, for example, contain high levels of phenolic compounds like quercetin...

  12. Rational Pathway Engineering of Type I Fatty Acid Synthase Allows the Biosynthesis of Triacetic Acid Lactone from D-Glucose in Vivo

    E-Print Network [OSTI]

    Zhao, Huimin

    *, Department of Chemical and Biomolecular Engineering, UniVersity of Illinois, 600 South Mathews AVenue, Urbana, Illinois 61801, and Department of Chemistry, Michigan State UniVersity, 528 Chemistry Building, East-B that is heterologously expressed in a microorganism would lead to TAL formation in vivo. To test this hypothesis, we

  13. Synthesis of new, single-isomer quaternary ammonium derivatives of beta-cyclodextrin for electrophoretic enantiomer separations 

    E-Print Network [OSTI]

    Nzeadibe, Kingsley C. I.

    2007-09-17

    by 1D and 2D NMR and massBoth cationic CD derivatives have been used for the separation of the enantiomers of strong acid, weak acid, weak base, ampholytic, and neutral analytes by capillary electrophoresis. Because the charge state of these cationic...

  14. Nucleic Acid Database: a Repository of Three-Dimensional Information about Nucleic Acids

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Berman, H. M.; Olson, W. K.; Beveridge, D. L.; Westbrook, J.; Gelbin, A.; Demeny, T.; Hsieh, S. H.; Srinivasan, A. R.; Schneider, B.

    The Nucleic Acid Database (NDB) provides 3-D structural information about nucleic acids.  It is a relational database designed to facilitate the easy search for nucleic acid structures using any of the stored primary or derived structural features. Reports can then be created describing any properties of the selected structures and structures may be viewed in several different formats, including the mmCIF format, the NDB Atlas format, the NDB coordinate format, or the PDB coordinate format. Browsing structure images created directly from coordinates in the repository can also be done. More than 7000 structures have been released as of May 2014. This website also includes a number of specialized tools and interfaces. The NDB Project is funded by the National Institutes of Health and has been funded by the National Science Foundation and the Department of Energy in the past.

  15. Effect of acetic acid on lipid accumulation by glucose-fed activated sludge cultures

    SciTech Connect (OSTI)

    Mondala, Andro; Hernandez, Rafael; French, Todd; McFarland, Linda; Sparks, Darrell; Holmes, William; Haque, Monica

    2012-01-01

    The effect of acetic acid, a lignocellulose hydrolysis by-product, on lipid accumulation by activated sludge cultures grown on glucose was investigated. This was done to assess the possible application of lignocellulose as low-cost and renewable fermentation substrates for biofuel feedstock production. Results: Biomass yield was reduced by around 54% at a 2 g L -1 acetic acid dosage but was increased by around 18% at 10 g L -1 acetic acid dosage relative to the control run. The final gravimetric lipid contents at 2 and 10 g L -1 acetic acid levels were 12.5 ���± 0.7% and 8.8 ���± 3.2% w/w, respectively, which were lower than the control (17.8 ���± 2.8% w/w). However, biodiesel yields from activated sludge grown with acetic acid (5.6 ���± 0.6% w/w for 2 g L -1 acetic acid and 4.2 ���± 3.0% w/w for 10 g L -1 acetic acid) were higher than in raw activated sludge (1-2% w/w). The fatty acid profiles of the accumulated lipids were similar with conventional plant oil biodiesel feedstocks. Conclusions: Acetic acid enhanced biomass production by activated sludge at high levels but reduced lipid production. Further studies are needed to enhance acetic acid utilization by activated sludge microorganisms for lipid biosynthesis.

  16. Derivative

    National Nuclear Security Administration (NNSA)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of NaturalDukeWakefield Municipal GasAdministration Medal01 Sandia4) AugustA. GeographicYucca fault (Class A)10 |

  17. Process for the synthesis of unsaturated alcohols

    SciTech Connect (OSTI)

    Maughon, Bob R.; Burdett, Kenneth A.; Lysenko, Zenon

    2007-02-13

    A process of preparing an unsaturated alcohol (olefin alcohol), such as, a homo-allylic mono-alcohol or homo-allylic polyol, involving protecting a hydroxy-substituted unsaturated fatty acid or fatty acid ester, such as methyl ricinoleate, derived from a seed oil, to form a hydroxy-protected unsaturated fatty acid or fatty acid ester; homo-metathesizing or cross-metathesizing the hydroxy-protected unsaturated fatty acid or fatty acid ester to produce a product mixture containing a hydroxy-protected unsaturated metathesis product; and deprotecting the hydroxy-protected unsaturated metathesis product under conditions sufficient to prepare the unsaturated alcohol. Preferably, methyl ricinoleate is converted by cross-metathesis or homo-metathesis into the homo-allylic mono-alcohol 1-decene-4-ol or the homo-allylic polyol 9-octadecene-7,12-diol, respectively.

  18. Beta-alanine/alpha-ketoglutarate aminotransferase for 3-hydroxypropionic acid production

    DOE Patents [OSTI]

    Jessen, Holly Jean (Chanhassen, MN); Liao, Hans H. (Eden Prairie, MN); Gort, Steven John (Apple Valley, MN); Selifonova, Olga V. (Plymouth, MN)

    2011-10-04

    The present disclosure provides novel beta-alanine/alpha ketoglutarate aminotransferase nucleic acid and protein sequences having increased biological activity. Also provided are cells containing such enzymes, as well as methods of their use, for example to produce malonyl semialdehyde and downstream products thereof, such as 3-hydroxypropionic acid and derivatives thereof.

  19. Beta-alanine/alpha-ketoglutarate aminotransferase for 3-hydroxypropionic acid production

    DOE Patents [OSTI]

    Jessen, Holly Jean; Liao, Hans H; Gort, Steven John; Selifonova, Olga V

    2014-11-18

    The present disclosure provides novel beta-alanine/alpha ketoglutarate aminotransferase nucleic acid and protein sequences having increased biological activity. Also provided are cells containing such enzymes, as well as methods of their use, for example to produce malonyl semialdehyde and downstream products thereof, such as 3-hydroxypropionic acid and derivatives thereof.

  20. Sol-gel derived sorbents

    DOE Patents [OSTI]

    Sigman, Michael E.; Dindal, Amy B.

    2003-11-11

    Described is a method for producing copolymerized sol-gel derived sorbent particles for the production of copolymerized sol-gel derived sorbent material. The method for producing copolymerized sol-gel derived sorbent particles comprises adding a basic solution to an aqueous metal alkoxide mixture for a pH.ltoreq.8 to hydrolyze the metal alkoxides. Then, allowing the mixture to react at room temperature for a precalculated period of time for the mixture to undergo an increased in viscosity to obtain a desired pore size and surface area. The copolymerized mixture is then added to an immiscible, nonpolar solvent that has been heated to a sufficient temperature wherein the copolymerized mixture forms a solid upon the addition. The solid is recovered from the mixture, and is ready for use in an active sampling trap or activated for use in a passive sampling trap.

  1. Magnetic cellulose-derivative structures

    DOE Patents [OSTI]

    Walsh, Myles A. (Falmouth, MA); Morris, Robert S. (Fairhaven, MA)

    1986-09-16

    Structures to serve as selective magnetic sorbents are formed by dissolving a cellulose derivative such as cellulose triacetate in a solvent containing magnetic particles. The resulting solution is sprayed as a fine mist into a chamber containing a liquid coagulant such as n-hexane in which the cellulose derivative is insoluble but in which the coagulant is soluble or miscible. On contact with the coagulant, the mist forms free-flowing porous magnetic microspheric structures. These structures act as containers for the ion-selective or organic-selective sorption agent of choice. Some sorbtion agents can be incorporated during the manufacture of the structure.

  2. Magnetic cellulose-derivative structures

    DOE Patents [OSTI]

    Walsh, M.A.; Morris, R.S.

    1986-09-16

    Structures to serve as selective magnetic sorbents are formed by dissolving a cellulose derivative such as cellulose triacetate in a solvent containing magnetic particles. The resulting solution is sprayed as a fine mist into a chamber containing a liquid coagulant such as n-hexane in which the cellulose derivative is insoluble but in which the coagulant is soluble or miscible. On contact with the coagulant, the mist forms free-flowing porous magnetic microspheric structures. These structures act as containers for the ion-selective or organic-selective sorption agent of choice. Some sorption agents can be incorporated during the manufacture of the structure. 3 figs.

  3. Oral Probiotic Microcapsule Formulation Ameliorates Non-Alcoholic Fatty Liver Disease in Bio F1B Golden Syrian Hamsters

    E-Print Network [OSTI]

    Bhathena, Jasmine; Martoni, Christopher; Kulamarva, Arun; Tomaro-Duchesneau, Catherine; Malhotra, Meenakshi; Paul, Arghya; Urbanska, Aleksandra Malgorzata; Prakash, Satya

    2013-03-12

    The beneficial effect of a microencapsulated feruloyl esterase producing Lactobacillus fermentum ATCC 11976 formulation for use in non-alcoholic fatty liver disease (NAFLD) was investigated. For which Bio F1B Golden Syrian hamsters were fed a...

  4. DERIVED CATEGORIES AND TILTING BERNHARD KELLER

    E-Print Network [OSTI]

    Keller, Bernhard - Institut de Mathématiques de Jussieu, Université Paris 7

    DERIVED CATEGORIES AND TILTING BERNHARD KELLER Abstract. We review the basic definitions of derived categories and deri* *ved functors. We that each tilting triple yields an* * equiv- alence between derived categories. We establish its

  5. Quaternion Derivatives: The GHR Calculus

    E-Print Network [OSTI]

    Dongpo Xu; Cyrus Jahanchahi; Clive C. Took; Danilo P. Mandic

    2014-09-25

    Quaternion derivatives in the mathematical literature are typically defined only for analytic (regular) functions. However, in engineering problems, functions of interest are often real-valued and thus not analytic, such as the standard cost function. The HR calculus is a convenient way to calculate formal derivatives of both analytic and non-analytic functions of quaternion variables, however, both the HR and other functional calculus in quaternion analysis have encountered an essential technical obstacle, that is, the traditional product rule is invalid due to the non- commutativity of the quaternion algebra. To address this issue, a generalized form of the HR derivative is proposed based on a general orthogonal system. The so introduced generalization, called the generalized HR (GHR) calculus, encompasses not just the left- and right-hand versions of quaternion derivative, but also enables solutions to some long standing problems, such as the novel product rule, the chain rule, the mean-valued theorem and Taylor's theorem. At the core of the proposed approach is the quaternion rotation, which can naturally be applied to other functional calculi in non-commutative settings. Examples on using the GHR calculus in adaptive signal processing support the analysis.

  6. Synthesis of acid addition salt of delta-aminolevulinic acid from 5-bromo levulinic acid esters

    DOE Patents [OSTI]

    Moens, Luc (Lakewood, CO)

    2003-06-24

    A process of preparing an acid addition salt of delta-aminolevulinc acid comprising: a) dissolving a lower alkyl 5-bromolevulinate and hexamethylenetetramine in a solvent selected from the group consisting of water, ethyl acetate, chloroform, acetone, ethanol, tetrahydrofuran and acetonitrile, to form a quaternary ammonium salt of the lower alkyl 5-bromolevulinate; and b) hydrolyzing the quaternary ammonium salt with an inorganic acid to form an acid addition salt of delta-aminolevulinic acid.

  7. DERIVATION OF STOCHASTIC ACCELERATION MODEL CHARACTERISTICS FOR...

    Office of Scientific and Technical Information (OSTI)

    DERIVATION OF STOCHASTIC ACCELERATION MODEL CHARACTERISTICS FOR SOLAR FLARES FROM RHESSI HARD X-RAY OBSERVATIONS Citation Details In-Document Search Title: DERIVATION OF STOCHASTIC...

  8. Derived Hom-Tensor adjointness. Local duality.

    E-Print Network [OSTI]

    2009-02-07

    Lectures on Grothendieck Duality. II: Derived Hom-Tensor adjointness. Local duality. Joseph Lipman. February 16, 2009. Contents. 1 Left-derived functors.

  9. Macrobiotic Vertical Transport of Litter Derived Carbon

    E-Print Network [OSTI]

    Post, Wilfred M.

    Macrobiotic Vertical Transport of Litter Derived Carbon (Earthworm Phase) Mac Callaham Corey Babb in each treatment Sampling #12;Macrobiotic Vertical Transport of Litter Derived Carbon (millipede phase

  10. Limited demand seen for scrubber-derived fertilizers

    SciTech Connect (OSTI)

    Not Available

    1985-12-01

    By-product marketability of scrubber-derived materials to the fertilizer industry will likely make only a small contribution to the acid rain problem. Those who claim the ammonia-based flue-gas materials will have an economic market cite the Bhara process undergoing testing at Indianapolis Power and Light for the removal of nitrogen oxides and sulfur dioxide, but fertilizer spokesmen feel the market will be limited to certain areas in the Midwest and the Pacific Northwest. This would reduce economic benefits. The Ebara material is easier to handle than ammonia, and should have a competitive price.

  11. Acidizing of Sandstone Reservoirs Using HF and Organic Acids 

    E-Print Network [OSTI]

    Yang, Fei

    2012-10-19

    Mud acid, which is composed of HCl and HF, is commonly used to remove the formation damage in sandstone reservoirs. However, many problems are associated with HCl, especially at high temperatures. Formic-HF acids have served as an alternative...

  12. Organic derivatives of zirconium phosphate 

    E-Print Network [OSTI]

    Fine, Steven Beryl

    1980-01-01

    ] . Zirconium phosphate is customarily thought of as a cation exchanger. But zirconium phosphate can also be an anion exchanger in its ester form or its regular form [12, 5]. When the ethanol ester of Y-zirconium phosphate is refluxed in a solution of sodium... dihydrogen phosphate, it forms phase P, which is the fully sodium- exchanged form of Y-zirconium phosphate [12]. When this is exchanged with protons via hydrochloric acid, Y-zirconium phosphate is formed. The reaction is: Zr(O3POCHZCHZOH)2 + 2NaHZP04 = Z...

  13. Acid Placement in Acid Jetting Treatments in Long Horizontal Wells 

    E-Print Network [OSTI]

    Sasongko, Hari

    2012-07-16

    In the Middle East, extended reach horizontal wells (on the order of 25,000 feet of horizontal displacement) are commonly acid stimulated by jetting acid out of drill pipe. The acid is jetted onto the face of the openhole wellbore as the drill pipe...

  14. Acid placement and coverage in the acid jetting process 

    E-Print Network [OSTI]

    Mikhailov, Miroslav I.

    2009-05-15

    Many open-hole acid treatments are being conducted by pumping acid through jetting ports placed at the end of coiled tubing or drill pipe. The filter-cake on the bore-hole is broken by the jet; the acid-soluble material is dissolved, creating...

  15. DERIVED CATEGORIES AND TILTING BERNHARD KELLER

    E-Print Network [OSTI]

    Keller, Bernhard - Institut de Mathématiques de Jussieu, Université Paris 7

    DERIVED CATEGORIES AND TILTING BERNHARD KELLER Abstract. We review the basic definitions of derived categories and derived functors. We illustrate them on simple but non trivial examples. Then we explain Happel's theorem which states that each tilting triple yields an equiv- alence between derived categories

  16. Synthesis and characterization of interfacially polymerized films of tetraphenylporphyrin derivatives

    SciTech Connect (OSTI)

    Li, W.; Wamser, C.C.

    1995-10-01

    Thin films of polymeric porphyrins have been made by interfacial polymerization of derivatives of tetraphenyl porphyrins, in particular by condensation of a dichloromethane solution of the acid chloride derivative (TCCPP) with a buffered aqueous solution of either the amine derivative (TAPP) or the phenol derivative (THPP). Spectroscopic and other studies are consistent with cross-linked polyamide or polyester network structures. The polyamide and polyester films display a novel asymmetry of functional groups on opposite sides of the film; excess amine (or hydroxyl) groups appear on one side of the film and excess carboxyl groups on the other. Film thickness can be correlated with the intensity of the UV-visible absorption spectrum, x (in nm) = 120 A{sub max} (at the Soret peak), with typical thicknesses in the range 10-500 nm, easily controlled by reaction time and conditions. Significantly thicker films (up to several {mu}m) can be prepared using an aliphatic diamine orpolyamine as the comonomer with TCCPP. Addition of 2, 6-lutidine to the organic phase substantially increases the rate of polymerization, which is especially useful for TAPP reactions. In addition, control experiments show that TCCPP with lutidine in CH{sub 2}Cl{sub 2} reacts at the interface with an aqueous pH 3 buffer, giving a very thin, easily hydrolyzed film, apparently due to anhydride linkages formed by condensation reactions with partially hydrolyzed TCCPP. 56 refs., 11 figs., 4 tabs.

  17. 2 SPRAY OILS--BEYOND 2000 Modern use of petroleum-derived oils as agricultural crop

    E-Print Network [OSTI]

    Agnello, Arthur M.

    2 SPRAY OILS--BEYOND 2000 Abstract Modern use of petroleum-derived oils as agricultural crop,buttheseweretoophytotoxic.Eventually, researchersconcentratedondistillatesintherangebetween kerosene and lubricating oils.Three basic classes of carbon structures present in petroleum oils (aromatics and other un- saturated components) in oils that were removable with sulfuric acid; the remainder

  18. LABORATORY DETECTION OF THIOCYANIC ACID HSCN

    SciTech Connect (OSTI)

    Bruenken, S.; Yu, Z.; Gottlieb, C. A.; McCarthy, M. C.; Thaddeus, P., E-mail: sbruenken@cfa.harvard.ed, E-mail: cgottlieb@cfa.harvard.ed, E-mail: mccarthy@cfa.harvard.ed, E-mail: pthaddeus@cfa.harvard.ed [Harvard-Smithsonian Center for Astrophysics, 60 Garden St., Cambridge, MA 02138 (United States)

    2009-12-01

    The rotational spectrum of thiocyanic acid HSCN, a highly polar isomer of the well-known astronomical molecule isothiocyanic acid HNCS, has been measured in two radio bands: in the centimeter-wave band by Fourier transform microwave spectroscopy in a molecular beam, and in the millimeter-wave band by long-path absorption spectroscopy in a low-pressure glow discharge. Twelve spectroscopic constants were derived from more than 60 a-type rotational transitions between 11 and 346 GHz with J up to 30 and K{sub a} <= 6, including seven centimeter-wave transitions with resolved hyperfine structure. With these constants the rotational spectrum in the K{sub a} = 0 and K{sub a} = 1 ladders-those most likely to be observed in space-can now be calculated up to 400 GHz with formal uncertainties of less than 0.2 km s{sup -1} in equivalent radial velocity. Thiocyanic acid was recently identified in Sgr B2 by Halfen et al. following the laboratory measurements, and there is possible evidence for it in cold dark clouds, with the implication that HSCN may be detectable in many galactic sources.

  19. Carboxylic acid sorption regeneration process

    DOE Patents [OSTI]

    King, C.J.; Poole, L.J.

    1995-05-02

    Carboxylic acids are sorbed from aqueous feedstocks into an organic liquid phase or onto a solid adsorbent. The acids are freed from the sorbent phase by treating it with aqueous alkylamine thus forming an alkylammonium carboxylate which is dewatered and decomposed to the desired carboxylic acid and the alkylamine. 10 figs.

  20. Pantothenic acid biosynthesis in zymomonas

    DOE Patents [OSTI]

    Tao, Luan; Tomb, Jean-Francois; Viitanen, Paul V.

    2014-07-01

    Zymomonas is unable to synthesize pantothenic acid and requires this essential vitamin in growth medium. Zymomonas strains transformed with an operon for expression of 2-dehydropantoate reductase and aspartate 1-decarboxylase were able to grow in medium lacking pantothenic acid. These strains may be used for ethanol production without pantothenic acid supplementation in seed culture and fermentation media.

  1. Mutant E. coli strain with increased succinic acid production

    DOE Patents [OSTI]

    Donnelly, Mark (Warrenville, IL); Millard, Cynthia S. (Plainfield, IL); Stols, Lucy (Woodridge, IL)

    1998-01-01

    A method for isolating succinic acid producing bacteria is provided comprising increasing the biomass of an organism which lacks the ability to catabolize pyruvate, and then subjecting the biomass to glucose-rich medium in an anaerobic environment to enable pyruvate-catabolizing mutants to grow. The invention also provides for a mutant that produces high amounts of succinic acid, which as been derived from a parent which lacked the genes for pyruvate formate lyase and lactate dehydrogenase, and which belongs to the E.coli Group of Bacteria.

  2. Mutant E. coli strain with increased succinic acid production

    DOE Patents [OSTI]

    Donnelly, Mark; Millard, Cynthia S.; Stols, Lucy

    2001-09-25

    A method for isolating succinic acid producing bacteria is provided comprising increasing the biomass of an organism which lacks the ability to catabolize pyruvate, and then subjecting the biomass to glucose-rich medium in an anaerobic environment to enable pyruvate-catabolizing mutants to grow. The invention also provides for a mutant that produces high amounts of succinic acid, which has been derived from a parent which lacked the genes for pyruvate formate lyase and lactate dehydrogenase, and which belongs to the E.coli Group of Bacteria.

  3. Mutant E. coli strain with increased succinic acid production

    DOE Patents [OSTI]

    Donnelly, Mark (Warrenville, IL); Millard, Cynthia S. (Plainfield, IL); Stols, Lucy (Woodridge, IL)

    2002-01-01

    A method for isolating succinic acid producing bacteria is provided comprising increasing the biomass of an organism which lacks the ability to catabolize pyruvate, and then subjecting the biomass to glucose-rich medium in an anaerobic environment to enable pyruvate-catabolizing mutants to grow. The invention also provides for a mutant that produces high amounts of succinic acid, which has been derived from a parent which lacked the genes for pyruvate formate lyase and lactate dehydrogenase, and which belongs to the E.coli Group of Bacteria.

  4. Binder enhanced refuse derived fuel

    DOE Patents [OSTI]

    Daugherty, Kenneth E. (Lewisville, TX); Venables, Barney J. (Denton, TX); Ohlsson, Oscar O. (Naperville, IL)

    1996-01-01

    A refuse derived fuel (RDF) pellet having about 11% or more particulate calcium hydroxide which is utilized in a combustionable mixture. The pellets are used in a particulate fuel bring a mixture of 10% or more, on a heat equivalent basis, of the RDF pellet which contains calcium hydroxide as a binder, with 50% or more, on a heat equivalent basis, of a sulphur containing coal. Combustion of the mixture is effective to produce an effluent gas from the combustion zone having a reduced SO.sub.2 and polycyclic aromatic hydrocarbon content of effluent gas from similar combustion materials not containing the calcium hydroxide.

  5. Composition for nucleic acid sequencing

    DOE Patents [OSTI]

    Korlach, Jonas (Ithaca, NY); Webb, Watt W. (Ithaca, NY); Levene, Michael (Ithaca, NY); Turner, Stephen (Ithaca, NY); Craighead, Harold G. (Ithaca, NY); Foquet, Mathieu (Ithaca, NY)

    2008-08-26

    The present invention is directed to a method of sequencing a target nucleic acid molecule having a plurality of bases. In its principle, the temporal order of base additions during the polymerization reaction is measured on a molecule of nucleic acid, i.e. the activity of a nucleic acid polymerizing enzyme on the template nucleic acid molecule to be sequenced is followed in real time. The sequence is deduced by identifying which base is being incorporated into the growing complementary strand of the target nucleic acid by the catalytic activity of the nucleic acid polymerizing enzyme at each step in the sequence of base additions. A polymerase on the target nucleic acid molecule complex is provided in a position suitable to move along the target nucleic acid molecule and extend the oligonucleotide primer at an active site. A plurality of labelled types of nucleotide analogs are provided proximate to the active site, with each distinguishable type of nucleotide analog being complementary to a different nucleotide in the target nucleic acid sequence. The growing nucleic acid strand is extended by using the polymerase to add a nucleotide analog to the nucleic acid strand at the active site, where the nucleotide analog being added is complementary to the nucleotide of the target nucleic acid at the active site. The nucleotide analog added to the oligonucleotide primer as a result of the polymerizing step is identified. The steps of providing labelled nucleotide analogs, polymerizing the growing nucleic acid strand, and identifying the added nucleotide analog are repeated so that the nucleic acid strand is further extended and the sequence of the target nucleic acid is determined.

  6. Invasive cleavage of nucleic acids

    DOE Patents [OSTI]

    Prudent, James R. (Madison, WI); Hall, Jeff G. (Madison, WI); Lyamichev, Victor I. (Madison, WI); Brow, Mary Ann D. (Madison, WI); Dahlberg, James E. (Madison, WI)

    2002-01-01

    The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

  7. Transpiration and water use by cotton plants as affected by the soil application of fatty alcohols 

    E-Print Network [OSTI]

    Rhoads, Frederick Milton

    1963-01-01

    on tz snspl: ' icn cont~ oi is almost non-existent for cotton. The reseat'ch zepo 'ted berin is one. pa. -. of a joint resea h program which is being conducred by t?e Texas Agriculcur=l Experimen Station and the Procter and Gamble Company fo. de... the fatty alcohols were incuba ed in moist soil for the pre- scribed length of time, the soil was dried in an oven, pulverized by grind- ing, placed in marked containers, and shipped to lfr. heal Thompson, of The Procter and Gamble Company (Pecos, Texas...

  8. Total Acid Value Titration of Hydrotreated Biomass Fast Pyrolysis Oil: Determination of Carboxylic Acids and Phenolics with Multiple End-Point Detection

    SciTech Connect (OSTI)

    Christensen, E.; Alleman, T. L.; McCormick, R. L.

    2013-01-01

    Total acid value titration has long been used to estimate corrosive potential of petroleum crude oil and fuel oil products. The method commonly used for this measurement, ASTM D664, utilizes KOH in isopropanol as the titrant with potentiometric end point determination by pH sensing electrode and Ag/AgCl reference electrode with LiCl electrolyte. A natural application of the D664 method is titration of pyrolysis-derived bio-oil, which is a candidate for refinery upgrading to produce drop in fuels. Determining the total acid value of pyrolysis derived bio-oil has proven challenging and not necessarily amenable to the methodology employed for petroleum products due to the different nature of acids present. We presented an acid value titration for bio-oil products in our previous publication which also utilizes potentiometry using tetrabutylammonium hydroxide in place of KOH as the titrant and tetraethylammonium bromide in place of LiCl as the reference electrolyte to improve the detection of these types of acids. This method was shown to detect numerous end points in samples of bio-oil that were not detected by D664. These end points were attributed to carboxylic acids and phenolics based on the results of HPLC and GC-MS studies. Additional work has led to refinement of the method and it has been established that both carboxylic acids and phenolics can be determined accurately. Use of pH buffer calibration to determine half-neutralization potentials of acids in conjunction with the analysis of model compounds has allowed us to conclude that this titration method is suitable for the determination of total acid value of pyrolysis oil and can be used to differentiate and quantify weak acid species. The measurement of phenolics in bio-oil is subject to a relatively high limit of detection, which may limit the utility of titrimetric methodology for characterizing the acidic potential of pyrolysis oil and products.

  9. Spontaneous and reversible interaction of vanadium(V) oxyanions with amine derivatives

    SciTech Connect (OSTI)

    Crans, D.C.; Shin, P.K.

    1988-05-18

    The interaction between vanadate and tri- or tetradentate ethanolamine derivatives has been studied by using /sup 51/V NMR spectroscopy. The reactions occur spontaneously in aqueous solutions, at ambient temperatures and in the physiological pH range. In addition to one amine group and one hydroxyl group, the ethanolamine derivative should contain a third and/or fourth functionality that is an alcohol, a carboxylic acid, a phosphonium acid, or an amine. The reactions are highly dependent on pH, concentrations of monomeric vanadate, amine. The stability constants for the complexes are minimum orders of magnitude greater than those found for vanadate derivatives of corresponding ether derivatives, and the high stability is associated with the central nitrogen. Only one vanadium complex is formed in substantial amounts in the reaction of ethanolamine derivatives with vanadate, and that complex is mononuclear in vanadium. Several of the ethanolamine derivatives that form complexes are commonly used buffers in biological and biomedical studies in vitro. 22 refs., 10 figs., 7 tabs.

  10. A study on real estate derivatives

    E-Print Network [OSTI]

    Lim, Jong Yoon, S.M. Massachusetts Institute of Technology

    2006-01-01

    All major asset classes including stocks and bonds have a well developed derivative market. Derivatives enable counterparties to reflect a view on a particular market, without having to trade the underlying asset. This ...

  11. Macrobiotic Vertical Transport of Litter Derived Carbon

    E-Print Network [OSTI]

    Post, Wilfred M.

    Macrobiotic Vertical Transport of Litter Derived Carbon (UPDATE) Mac Callaham Corey Babb Paul vertical transport of litter derived carbon-millipede phase More germane to upland sites Sampled uplands

  12. Equivalence of Conventionally-Derived and Parthenote-Derived Human Embryonic Stem Cells

    E-Print Network [OSTI]

    2011-01-01

    Equivalence of Conventionally-Derived andParthenote- Derived Human Embryonic Stem Cells Julie V.cell (hESC) lines can be derived via multiple means, it is

  13. Nucleic acid detection methods

    DOE Patents [OSTI]

    Smith, C.L.; Yaar, R.; Szafranski, P.; Cantor, C.R.

    1998-05-19

    The invention relates to methods for rapidly determining the sequence and/or length a target sequence. The target sequence may be a series of known or unknown repeat sequences which are hybridized to an array of probes. The hybridized array is digested with a single-strand nuclease and free 3{prime}-hydroxyl groups extended with a nucleic acid polymerase. Nuclease cleaved heteroduplexes can be easily distinguish from nuclease uncleaved heteroduplexes by differential labeling. Probes and target can be differentially labeled with detectable labels. Matched target can be detected by cleaving resulting loops from the hybridized target and creating free 3-hydroxyl groups. These groups are recognized and extended by polymerases added into the reaction system which also adds or releases one label into solution. Analysis of the resulting products using either solid phase or solution. These methods can be used to detect characteristic nucleic acid sequences, to determine target sequence and to screen for genetic defects and disorders. Assays can be conducted on solid surfaces allowing for multiple reactions to be conducted in parallel and, if desired, automated. 18 figs.

  14. Nucleic Acid Detection Methods

    DOE Patents [OSTI]

    Smith, Cassandra L. (Boston, MA); Yaar, Ron (Brookline, MA); Szafranski, Przemyslaw (Boston, MA); Cantor, Charles R. (Boston, MA)

    1998-05-19

    The invention relates to methods for rapidly determining the sequence and/or length a target sequence. The target sequence may be a series of known or unknown repeat sequences which are hybridized to an array of probes. The hybridized array is digested with a single-strand nuclease and free 3'-hydroxyl groups extended with a nucleic acid polymerase. Nuclease cleaved heteroduplexes can be easily distinguish from nuclease uncleaved heteroduplexes by differential labeling. Probes and target can be differentially labeled with detectable labels. Matched target can be detected by cleaving resulting loops from the hybridized target and creating free 3-hydroxyl groups. These groups are recognized and extended by polymerases added into the reaction system which also adds or releases one label into solution. Analysis of the resulting products using either solid phase or solution. These methods can be used to detect characteristic nucleic acid sequences, to determine target sequence and to screen for genetic defects and disorders. Assays can be conducted on solid surfaces allowing for multiple reactions to be conducted in parallel and, if desired, automated.

  15. Genetic Optimization Using Derivatives Jasjeet S. Sekhon

    E-Print Network [OSTI]

    Sekhon, Jasjeet S.

    Genetic Optimization Using Derivatives by Jasjeet S. Sekhon and Walter R. Mebane, Jr. Draft://macht.arts.cornell.edu/wrm1/. #12;Abstract Genetic Optimization Using Derivatives We describe a new computer program unconstrained optimization problems. The program, called GENOUD (GENetic Optimization Using Derivatives

  16. The Fourth Partial Derivative In Transport Dynamics

    E-Print Network [OSTI]

    Trinh Khanh Tuoc

    2010-01-11

    A new fourth partial derivative is introduced for the study of transport dynamics. It is a Lagrangian partial derivative following the path of diffusion, not the path of convection. Use of this derivative decouples the effect of diffusion and convection and simplifies the analysis of transport processes.

  17. Development of the beta-pressure derivative 

    E-Print Network [OSTI]

    Hosseinpour-Zoonozi, Nima

    2007-04-25

    The proposed work provides a new definition of the pressure derivative function [that is the �²-derivative function, ��p �²d(t)], which is defined as the derivative of the logarithm of pressure drop data with respect ...

  18. Postgraduate Scholarship Pricing temperature derivatives and modelling

    E-Print Network [OSTI]

    Banaji,. Murad

    the volumetric risk of the energy units sold, rather than the price risk of each unit. Weather derivativesPostgraduate Scholarship Pricing temperature derivatives and modelling the market price of risk: Pricing temperature derivatives and modelling the market price of risk. Main Supervisor: A. Alexandridis

  19. Derived Equivalences of Orders Alexander Zimmermann

    E-Print Network [OSTI]

    Zimmermann, Alexander

    Derived Equivalences of Orders Alexander Zimmermann Abstract Using the result of Roggenkamp with Steffen K¨onig [17]. The last section gives a definition and first properties of Picard groups of derived Derived categories arose first in algebraic geometry in the early 60's and proved there to be a powerful

  20. Derived critical loci I -Basics Gabriele Vezzosi

    E-Print Network [OSTI]

    Vezzosi, Gabriele

    Derived critical loci I - Basics Gabriele Vezzosi Dipartimento di Sistemi ed Informatica Universit`a di Firenze Italy Notes ­ September 2011 Contents 1 Introduction 1 2 Koszul complexes and derived zero . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 2.2 Affine derived zero loci . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 2

  1. HOCHSCHILD COHOMOLOGY AND DERIVED PICARD BERNHARD KELLER

    E-Print Network [OSTI]

    Keller, Bernhard - Institut de Mathématiques de Jussieu, Université Paris 7

    HOCHSCHILD COHOMOLOGY AND DERIVED PICARD GROUPS BERNHARD KELLER Dedicated to Idun Reiten- rived Picard group and deduce that it is preserved under derived equivalences. 1. Introduction groups of the bimodule A by itself or as morphisms from A to A[i] in the derived category D(Aop A) of A

  2. HOCHSCHILD COHOMOLOGY AND DERIVED PICARD BERNHARD KELLER

    E-Print Network [OSTI]

    Keller, Bernhard - Institut de Mathématiques de Jussieu, Université Paris 7

    HOCHSCHILD COHOMOLOGY AND DERIVED PICARD GROUPS BERNHARD KELLER Dedicated to Idun Reiten­ rived Picard group and deduce that it is preserved under derived equivalences. 1. Introduction extension groups of the bimodule A by itself or as morphisms from A to A[i] in the derived category D(A op

  3. Metabolic evolution of Escherichia coli strains that produce organic acids

    SciTech Connect (OSTI)

    Grabar, Tammy; Gong, Wei; Yocum, R Rogers

    2014-10-28

    This invention relates to the metabolic evolution of a microbial organism previously optimized for producing an organic acid in commercially significant quantities under fermentative conditions using a hexose sugar as sole source of carbon in a minimal mineral medium. As a result of this metabolic evolution, the microbial organism acquires the ability to use pentose sugars derived from cellulosic materials for its growth while retaining the original growth kinetics, the rate of organic acid production and the ability to use hexose sugars as a source of carbon. This invention also discloses the genetic change in the microorganism that confers the ability to use both the hexose and pentose sugars simultaneously in the production of commercially significant quantities of organic acids.

  4. Acidic gas capture by diamines

    DOE Patents [OSTI]

    Rochelle, Gary (Austin, TX); Hilliard, Marcus (Missouri City, TX)

    2011-05-10

    Compositions and methods related to the removal of acidic gas. In particular, the present disclosure relates to a composition and method for the removal of acidic gas from a gas mixture using a solvent comprising a diamine (e.g., piperazine) and carbon dioxide. One example of a method may involve a method for removing acidic gas comprising contacting a gas mixture having an acidic gas with a solvent, wherein the solvent comprises piperazine in an amount of from about 4 to about 20 moles/kg of water, and carbon dioxide in an amount of from about 0.3 to about 0.9 moles per mole of piperazine.

  5. Total-derivative supersymmetry breaking

    SciTech Connect (OSTI)

    Haba, Naoyuki; Uekusa, Nobuhiro

    2010-05-15

    On an interval compactification in supersymmetric theory, boundary conditions for bulk fields must be treated carefully. If they are taken arbitrarily following the requirement that a theory is supersymmetric, the conditions could give redundant constraints on the theory. We construct a supersymmetric action integral on an interval by introducing brane interactions with which total-derivative terms under the supersymmetry transformation become zero due to a cancellation. The variational principle leads equations of motion and also boundary conditions for bulk fields, which determine boundary values of bulk fields. By estimating mass spectrum, spontaneous supersymmetry breaking in this simple setup can be realized in a new framework. This supersymmetry breaking does not induce a massless R axion, which is favorable for phenomenology. It is worth noting that fermions in hyper-multiplet, gauge bosons, and the fifth-dimensional component of gauge bosons can have zero-modes (while the other components are all massive as Kaluza-Klein modes), which fits the gauge-Higgs unification scenarios.

  6. Acid Strength and Bifunctional Catalytic Behavior of Alloys Comprised of Noble Metals and Oxophilic Metal Promoters

    SciTech Connect (OSTI)

    Hibbitts, David D.; Tan, Qiaohua; Neurock, Matthew

    2014-06-01

    The promotion of metal catalysts with partially oxidized oxophilic MOx species, such as ReOx-promoted Rh, has been demonstrated to produce Brønsted acid sites that can promote hydrogenolysis of oxygenate intermediates such as those found in biomass-derived species. A wide variety of alloy compositions and structures are examined in this work to investigate strongly acidic promoters by using DFT-calculated deprotonation energies (DPE) as a measure of acid strength. Sites with the highest acid strength had DPE less than 1100 kJ mol-1, similar to DPE values of heteropolyacids or acid-containing zeolites, and were found on alloys composed of an oxophilic metal (such as Re or W) with a noble metal (such as Rh or Pt). NH3 adsorbs more strongly to sites with increasing acid strength and the activation barriers for acid-catalyzed ring opening of a furan ring decrease with increasing acid strength, which was also shown to be stronger for OH acid sites bound to multiple oxophilic metal atoms in a three-fold configuration rather than OH sites adsorbed in an atop configuration on one oxophilic metal, indicating that small MOx clusters may yield sites with the highest acid strength.

  7. The utilization of tricarboxylic acid cycle acids and the uptake of succinic acid by Neurospora crassa 

    E-Print Network [OSTI]

    Gilliland, Patti Lynn

    1978-01-01

    molecules not directly involved in the TCA cycle, aspartate and glutamate, have been the subject of several investigations, primarily in relation to amino acid transport. The uptake of the dicarboxylic amino acids is fre- quently studied in conjunction... with the uptake of the structurally similar TCA cycle compounds (22, 44, 76). In 1968, Jacobson snd Metzenberg (29) reported that aspartate and glutamate uptake by N. crassa was gene-controlled. Pall (44) has described a mycelial amino acid transport system...

  8. Anti-inflammatory Activity of New Guaiane Acid Derivatives from Achillea Coarctata

    E-Print Network [OSTI]

    Paré, Paul W.

    ]. An essential oil is traditionally obtained for medical uses [4]. Previous chemical studies on A. coarctata revealed a wide range of sesquiterpene lactones [5]. Essential oils [6] and flavonoids [7] have also been oil { 25 D + 5.2 (c 1.99, CHCl3)}. The IR spectrum showed an absorption band at 3448 (OH), 2852 (C

  9. Advanced electrodialysis and pervaporation for fermentation-derived organic acids production.

    SciTech Connect (OSTI)

    Tsai, S. P.

    1998-11-18

    Lactate esters produced from carbohydrate have potential markets as nontoxic replacements for halogenated and toxic solvents and as feedstocks for large-volume chemicals and polymers. Argonne National Laboratory has developed a novel process for the production of high-purity lactate esters from carbohydrate. The process uses advanced electrodialysis and pervaporation technologies to overcome major technical barriers in product separation; more specifically, the process involves cation elimination without the generation of salt waste and efficient esterification for final purification. This patented process requires little energy input, is highly efficient and selective, eliminates the large volumes of salt waste produced by conventional processes, and significantly reduces manufacturing costs. The enabling membrane separation technologies make it technically and commercially feasible for lactate esters to penetrate the potential markets.

  10. CER4 Encodes an Alcohol-Forming Fatty Acyl-Coenzyme A Reductase Involved in Cuticular Wax Production

    E-Print Network [OSTI]

    Kunst, Ljerka

    CER4 Encodes an Alcohol-Forming Fatty Acyl-Coenzyme A Reductase Involved in Cuticular Wax surfaces of land plants. It is composed of a cutin polymer matrix and waxes. Cuticular waxes are complex and characterization of CER4, a wax bio- synthetic gene from Arabidopsis (Arabidopsis thaliana). Arabidopsis cer4

  11. Preliminary results of a comparison between 4 pig breeds in chemical composition of fatty tissue and intramuscular fat content

    E-Print Network [OSTI]

    Boyer, Edmond

    and relatively slow growth rate, gave a fatty tissue with higher water content, and the lipids of this tissue water content did not exceed 12 p. 100 in the other breeds. Backfat with so much water was not suitable hand, carcass fatness and growth rate on the other hand. Water content and degree of unsaturation

  12. A ACID RAIN Audrey Gibson

    E-Print Network [OSTI]

    Toohey, Darin W.

    account for about 70 percent of annual SO2 emissions and 30 percent of NOx emissions in the United States acid and nitric acid. Sunlight increases the rate of most of these reactions. Electric utility plants and NOx are harmful to the lungs and can cause disease and premature death. Thursday, April 29, 2010 #12

  13. Characterization of ?-carrageenan and its derivative based green polymer electrolytes

    SciTech Connect (OSTI)

    Jumaah, Fatihah Najirah; Mobaraka, Nadhratun Naiim; Ahmad, Azizan; Ramli, Nazaruddin [School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600, Bangi, Selangor Darul Ehsan (Malaysia)

    2013-11-27

    The new types of green polymer electrolytes based on ?-carrageenan derivative have been prepared. ?-carrageenan act as precursor was reacted with monochloroacetic acid to produce carboxymethyl ?-carrageenan. The powders were characterized by Attenuated Total Reflection Fourier Transform infrared (ATR-FTIR) spectroscopy and {sup 1}H nuclear magnetic resonance (NMR) to confirm the substitution of targeted functional group in ?-carrageenan. The green polymer electrolyte based on ?-carrageenan and carboxymethyl ?-carrageenan was prepared by solution-casting technique. The films were characterized by electrochemical impedance spectroscopy to determine the ionic conductivity. The ionic conductivity ?-carrageenan film were higher than carboxymethyl ?-carrageenan which 4.87 ×10{sup ?6} S cm{sup ?1} and 2.19 ×10{sup ?8} S cm{sup ?1}, respectively.

  14. Nucleic acids encoding human trithorax protein

    DOE Patents [OSTI]

    Evans, Glen A. (Encinitas, CA); Djabali, Malek (Marseilles, FR); Selleri, Licia (Del Mar, CA); Parry, Pauline (San Diego, CA)

    2001-01-01

    In accordance with the present invention, there is provided an isolated peptide having the characteristics of human trithorax protein (as well as DNA encoding same, antisense DNA derived therefrom and antagonists therefor). The invention peptide is characterized by having a DNA binding domain comprising multiple zinc fingers and at least 40% amino acid identity with respect to the DNA binding domain of Drosophila trithorax protein and at least 70% conserved sequence with respect to the DNA binding domain of Drosophila trithorax protein, and wherein said peptide is encoded by a gene located at chromosome 11 of the human genome at q23. Also provided are methods for the treatment of subject(s) suffering from immunodeficiency, developmental abnormality, inherited disease, or cancer by administering to said subject a therapeutically effective amount of one of the above-described agents (i.e., peptide, antagonist therefor, DNA encoding said peptide or antisense DNA derived therefrom). Also provided is a method for the diagnosis, in a subject, of immunodeficiency, developmental abnormality, inherited disease, or cancer associated with disruption of chromosome 11 at q23.

  15. Extending Bauer's corollary to fractional derivatives

    E-Print Network [OSTI]

    David W. Dreisigmeyer; Peter M. Young

    2004-02-11

    We comment on the method of Dreisigmeyer and Young [D. W. Dreisigmeyer and P. M. Young, J. Phys. A \\textbf{36}, 8297, (2003)] to model nonconservative systems with fractional derivatives. It was previously hoped that using fractional derivatives in an action would allow us to derive a single retarded equation of motion using a variational principle. It is proven that, under certain reasonable assumptions, the method of Dreisigmeyer and Young fails.

  16. Producing Transportation Fuels via Photosynthetically-derived...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    technology using cyanobacteria. This technology has potential to produce biofuels and green chemicals (1) at cost that is competitive with conventional ethylene and derivatives...

  17. An extension of the classical derivative

    E-Print Network [OSTI]

    Diego Dominici

    2006-03-24

    We extend the usual definition of the derivative in a way that Calculus I students can easily comprehend and which allows calculations at branch points.

  18. Calibration by Optimization Without Using Derivatives

    E-Print Network [OSTI]

    Markus Lazar

    2015-03-06

    Mar 6, 2015 ... Abstract: Applications in engineering frequently require the ... to upper and lower bounds without relying on the knowledge of the derivative of f .

  19. Generalized Gravitational Entropy from Total Derivative Action

    E-Print Network [OSTI]

    Dong, Xi

    2015-01-01

    We investigate the generalized gravitational entropy from total derivative terms in the gravitational action. Following the method of Lewkowycz and Maldacena, we find that the generalized gravitational entropy from total derivatives vanishes. We compare our results with the work of Astaneh, Patrushev, and Solodukhin. We find that if total derivatives produced nonzero entropy, the holographic and the field-theoretic universal terms of entanglement entropy would not match. Furthermore, the second law of thermodynamics could be violated if the entropy of total derivatives did not vanish.

  20. Proceedings of refuse-derived fuel (RDF)

    SciTech Connect (OSTI)

    Saltiel, C. )

    1991-01-01

    This book contains proceedings of Refuse-Derived Fuel (RDF)-Quality. Standards and Processing. Topics covered include: An Overview of RDF Processing Systems: Current Status, Design Features, and Future Trends. The Impact of Recycling and Pre-Combustion Processing of Municipal Solid Waste on Fuel Properties and Steam Combustion. The Changing Role of Standards in the Marketing of RDF. Refuse Derived Fuel Quality Requirements for Firing in Utility, Industrial or Dedicated Boilers. Refuse-Derived Fuel Moisture Effects on Boiler Performance and Operability. Refuse Derived Fuels: Technology, Processing, Quality and Combustion Experiences.

  1. Biomass Derivatives Competitive with Heating Oil Costs.

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Biomass Derivatives Competitive with Heating Oil Costs Transportation fuel Heat or electricity * Data are from literature, except heating oil is adjusted from 2011 winter average *...

  2. Tetrahydroquinoline Derivatives as Potent and Selective Factor...

    Office of Scientific and Technical Information (OSTI)

    Derivatives as Potent and Selective Factor XIa Inhibitors Authors: Quan, Mimi L. ; Wong, Pancras C. ; Wang, Cailan ; Woerner, Francis ; Smallheer, Joanne M. ; Barbera, Frank...

  3. Derived Hom-Tensor adjointness. Local duality.

    E-Print Network [OSTI]

    2009-02-22

    Feb 16, 2009 ... 1 Left-derived functors. Tensor and Tor. 2 Hom -Tensor adjunction. 3 Abstract local duality. 4 Concrete local duality. 5 Residues and duality for ...

  4. Derived Hochschild Cohomology and Grothendieck Duality: local ...

    E-Print Network [OSTI]

    2008-05-24

    May 20, 2008 ... 6 Relative dualizing complexes in Commutative Algebra. 7 Back to schemes. Joseph Lipman (Purdue University). Derived Hochschild and ...

  5. Efficient Route to Highly Water-Soluble Aromatic Cyclic Hydroxamic Acid Ligands

    SciTech Connect (OSTI)

    Seitz, Michael; Raymond, Kenneth N.

    2008-02-06

    2-Hydroxyisoquinolin-1-one (1,2-HOIQO) is a new member of the important class of aromatic cyclic hydroxamic acid ligands which are widely used in metal sequestering applications and metal chelating therapy. The first general approach for the introduction of substituents at the aromatic ring of the chelating moiety is presented. As a useful derivative, the highly water-soluble sulfonic acid has been synthesized by an efficient route that allows general access to 1,2-HOQIO 3-carboxlic acid amides, which are the most relevant for applications.

  6. Metabolism of Thioctic Acid in Algae

    E-Print Network [OSTI]

    Grisebach, Hans; Fuller, R.C.; Calvin, M.

    1956-01-01

    METABOLISM OF THlOCTlC ACID IN ALGAE TWO-WEEK LOAN COPY ThisMETABOLISM OF THIOCTIC ACID IN ALGAE Hans Grisebach, R. , C.METABOLISM OF THIOCTIC ACID IN ALGAE Hans Grisebach, R. C.

  7. Modeling of the formation of short-chain acids in propane flames

    E-Print Network [OSTI]

    Battin-Leclerc, Frédérique; Jaffrezo, J L; Legrand, M

    2009-01-01

    In order to better understand their potential formation in combustion systems, a detailed kinetic mechanism for the formation of short-chain monocarboxylic acids, formic (HCOOH), acetic (CH3COOH), propionic (C2H5COOH) and propenic (C2H3COOH)) acids, has been developed. Simulations of lean (equivalence ratios from 0.9 to 0.48) laminar premixed flames of propane stabilized at atmospheric pressure with nitrogen as diluent have been performed. It was found that amounts up to 25 ppm of acetic acid, 15 ppm of formic acid and 1 ppm of C3 acid can be formed for some positions in the flames. Simulations showed that the more abundant C3 acid formed is propenic acid. A quite acceptable agreement has been obtained with the scarce results from the literature concerning oxygenated compounds, including aldehydes (CH2O, CH3CHO) and acids. A reaction pathways analysis demonstrated that each acid is mainly derived from the aldehyde of similar structure.

  8. A multi-period equilibrium pricing model of weather derivatives

    E-Print Network [OSTI]

    Lee, Yongheon; Oren, Shmuel S.

    2010-01-01

    Y. : Valuation and hedging of weather derivatives on monthlyJ. Risk 31. Yoo, S. : Weather derivatives and seasonaleffects and valuation of weather derivatives. Financ. Rev.

  9. A Multi-period Equilibrium Pricing Model of Weather Derivatives

    E-Print Network [OSTI]

    Lee, Yongheon; Oren, Shmuel S.

    2008-01-01

    2002). On modelling and pricing weather derivatives. Applied2003). Arbitrage-fee pricing of weather derivatives based onfects and valuation of weather derivatives. The Financial

  10. Endothelial influences enhance human pluripotent stem cell -derived cardiomyocyte maturation

    E-Print Network [OSTI]

    Wei, Karen A.

    2011-01-01

    Human embryonic stem cell-derived cardiomyocytes survive andet al. , Cardiomyocytes derived from human embryonic stemcardiac function by hESC-derived cardiomyocytes correlates

  11. INFORMATION ON MEMBRANES DERIVED FROM ELECTRICAL SURFACE CHARGE MEASUREMENTS

    E-Print Network [OSTI]

    Tenforde, Tom S.

    2012-01-01

    V/cm) for fi cell lines derived from both Swiss and Balb 148 INFORMATION ON MEMBRANES DERIVED FROM ELECTRICAL SURFACEINFORMATION ON MEMBRANES DERIVED FROM ELECTRICAL SURFACE

  12. The Direction of Optimal Skin Incisions Derived From Striae Distensae.

    E-Print Network [OSTI]

    Lemperle, Gottfried

    2015-01-01

    of Optimal Skin Incisions Derived from Striae Distensaestriae lines. These are derived from the striae compositecal incisions and scars derived from the Internet. Incision

  13. Superbase-derived protic ionic liquids

    DOE Patents [OSTI]

    Dai, Sheng; Luo, Huimin; Baker, Gary A.

    2013-09-03

    Protic ionic liquids having a composition of formula (A.sup.-)(BH.sup.+) wherein A.sup.- is a conjugate base of an acid HA, and BH.sup.+ is a conjugate acid of a superbase B. In particular embodiments, BH.sup.+ is selected from phosphazenium species and guanidinium species encompassed, respectively, by the general formulas: ##STR00001## The invention is also directed to films and membranes containing these protic ionic liquids, with particular application as proton exchange membranes for fuel cells.

  14. Derivatives of Isogeometric Functions on Rational Patches

    E-Print Network [OSTI]

    Jüttler, Bert

    patch in Rd , then the graph of an isogeometric function is a d-dimensional patch (a hyper and their derivatives. Given a geometry mapping as an n- dimensional NURBS patch in Rd , an isogeometric function for the derivatives of the isogeometric functions as parametric patches. We deal with domains represented by n-dimensional

  15. Carbonic Acid Shows Promise in Geology, Biology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The Surprising Secrets of Carbonic Acid Probing the Surprising Secrets of Carbonic Acid Berkeley Lab Study Holds Implications for Geological and Biological Processes October 23,...

  16. In vivo incorporation of unnatural amino acids

    DOE Patents [OSTI]

    Schultz, Peter G. (La Jolla, CA); Wang, Lei (San Diego, CA); Anderson, John Christopher (San Diego, CA); Chin, Jason W. (Cambridge, GB); Liu, David R. (Lexington, MA); Magliery, Thomas J. (North Haven, CT); Meggers, Eric L. (Philadelphia, PA); Mehl, Ryan Aaron (Lancaster, PA); Pastrnak, Miro (San Diego, CA); Santoro, Stephen William (Cambridge, MA); Zhang, Zhiwen (San Diego, CA)

    2012-05-08

    The invention provides methods and compositions for in vivo incorporation of unnatural amino acids. Also provided are compositions including proteins with unnatural amino acids.

  17. In vivo incorporation of unnatural amino acids

    DOE Patents [OSTI]

    Schultz, Peter (La Jolla, CA); Wang, Lei (San Diego, CA); Anderson, John Christopher (San Diego, CA); Chin, Jason W. (Cambridge, GB); Liu, David R. (Lexington, MA); Magliery, Thomas J. (North Haven, CT); Meggers, Eric (Philadelphia, PA); Mehl, Ryan Aaron (Lancaster, PA); Pastrnak, Miro (San Diego, CA); Santoro, Stephen William (Cambridge, MA); Zhang, Zhiwen (San Diego, CA)

    2011-03-29

    The invention provides methods and compositions for in vivo incorporation of unnatural amino acids. Also provided are compositions including proteins with unnatural amino acids.

  18. In vivo incorporation of unnatural amino acids

    DOE Patents [OSTI]

    Schultz, Peter (La Jolla, CA); Wang, Lei (San Diego, CA); Anderson, John Christopher (San Diego, CA); Chin, Jason W. (Cambridge, GB); Liu, David R. (Lexington, MA); Magliery, Thomas J. (North Haven, CT); Meggers, Eric (Philadelphia, PA); Mehl, Ryan Aaron (Lancaster, PA); Pastrnak, Miro (San Diego, CA); Santoro, Steven William (Cambridge, MA); Zhang, Zhiwen (San Diego, CA)

    2008-05-06

    The invention provides methods and compositions for in vivo incorporation of unnatural amino acids. Also provided are compositions including proteins with unnatural amino acids.

  19. In vivo incorporation of unnatural amino acids

    DOE Patents [OSTI]

    Schultz, Peter G. (La Jolla, CA); Wang, Lei (San Diego, CA); Anderson, John Christopher (San Diego, CA); Chin, Jason W. (Cambridge, GB); Liu, David R. (Lexington, MA); Magliery, Thomas J. (North Haven, CT); Meggers, Eric L. (Philadelphia, PA); Mehl, Ryan Aaron (Lancaster, PA); Pastrnak, Miro (San Diego, CA); Santoro, Stephen William (Cambridge, MA); Zhang, Zhiwen (San Diego, CA)

    2012-02-14

    The invention provides methods and compositions for in vivo incorporation of unnatural amino acids. Also provided are compositions including proteins with unnatural amino acids.

  20. In vivo incorporation of unnatural amino acids

    DOE Patents [OSTI]

    Schultz, Peter G. (La Jolla, CA); Wang, Lei (San Diego, CA); Anderson, John Christopher (San Diego, CA); Chin, Jason W. (Cambridge, GB); Liu, David R. (Lexington, MA); Magliery, Thomas J. (North Haven, CT); Meggers, Eric L. (Philadelphia, PA); Mehl, Ryan Aaron (Lancaster, PA); Pastrnak, Miro (San Diego, CA); Santoro, Stephen William (Cambridge, MA); Zhang, Zhiwen (San Diego, CA)

    2011-10-04

    The invention provides methods and compositions for in vivo incorporation of unnatural amino acids. Also provided are compositions including proteins with unnatural amino acids.

  1. In vivo incorporation of unnatural amino acids

    DOE Patents [OSTI]

    Schultz, Peter (La Jolla, CA); Wang, Lei (San Diego, CA); Anderson, John Christopher (San Diego, CA); Chin, Jason W. (Cambridge, GB); Liu, David R. (Lexington, MA); Magliery, Thomas J. (North Haven, CT); Meggers, Eric (Philadelphia, PA); Mehl, Ryan Aaron (Lancaster, PA); Pastrnak, Miro (San Diego, CA)

    2009-12-29

    The invention provides methods and compositions for in vivo incorporation of unnatural amino acids. Also provided are compositions including proteins with unnatural amino acids

  2. Integrating weather derivatives for managing risks

    SciTech Connect (OSTI)

    Bilski, B. [WeatherWise USA LLC, Pittsburgh, PA (United States)

    1999-11-01

    As deregulation and customer choice loom on the horizon, many energy utilities and other energy suppliers are scrambling to find new services that add value for consumers. Many are also seeking opportunities for increasing efficiency to ensure that costs remain competitive. Integrating weather derivatives with marketing programs and financial management can produce attractive new services and increase efficiency. Weather derivatives can be used to create innovative consumer services, such as a guaranteed annual energy bill which is unaffected by weather and energy price changes. They can also be used to protect the earnings of energy suppliers from one of their most significant financial risks, unpredictable weather. There are three basic types of weather derivatives available today. Option or insurance based derivatives (options), swaps or hedge based derivatives (swaps) and packages where other services are combined with one or both of the above.

  3. Controlling acid rain : policy issues

    E-Print Network [OSTI]

    Fay, James A.

    1983-01-01

    The policy and regulatory ramifications of U.S. acid rain control programs are examined; particularly, the alternative of a receptor-oriented strategy as constrasted to emission-oriented proposals (e.g., the Mitchell bill) ...

  4. Nitrate and Prussic Acid Poisoning 

    E-Print Network [OSTI]

    Stichler, Charles; Reagor, John C.

    2001-09-05

    Nitrate and prussic acid poisoning in cattle are noninfectious conditions that can kill livestock. This publication explains the causes and symptoms of these conditions as well as preventive measures and sampling and testing steps....

  5. Seasonalepisodic control of acid deposition

    E-Print Network [OSTI]

    Fay, James A.

    1988-01-01

    This report contains the climatological, technical and economic factors for episodic and seasonal control of emissions in existing power plants. Analyzing a large data set of acid deposition for the years 1982-85, we find ...

  6. Phosphonic acid based exchange resins

    DOE Patents [OSTI]

    Horwitz, E.P.; Alexandratos, S.D.; Gatrone, R.C.; Chiarizia, R.

    1995-09-12

    An ion exchange resin is described for extracting metal ions from a liquid waste stream. An ion exchange resin is prepared by copolymerizing a vinylidene diphosphonic acid with styrene, acrylonitrile and divinylbenzene. 10 figs.

  7. Phosphonic acid based exchange resins

    DOE Patents [OSTI]

    Horwitz, E. Philip (Naperville, IL); Alexandratos, Spiro D. (Knoxville, TN); Gatrone, Ralph C. (Naperville, IL); Chiarizia, Ronato (Oak Park, IL)

    1995-01-01

    An ion exchange resin for extracting metal ions from a liquid waste stream. An ion exchange resin is prepared by copolymerizing a vinylidene diphosphonic acid with styrene, acrylonitrile and divinylbenzene.

  8. Derived Differential Geometry Prof Joyce 14 lectures TT 2015 Overview

    E-Print Network [OSTI]

    Burton, Geoffrey R.

    1 Derived Differential Geometry Prof Joyce 14 lectures TT 2015 Overview Derived Differential Geometry is the study of derived smooth manifolds and orbifolds, where "derived" is in the sense

  9. Proportional-Integral-Derivative Control Derivative Filtering and Integral Anti-Windup

    E-Print Network [OSTI]

    Proportional-Integral-Derivative Control with Derivative Filtering and Integral Anti 2015 Neil Avenue, Columbus, OH 43210-1272 March 22, 2002 Abstract First, you will design a proportional controller to meet a given set of specifications. Then, you will design a proportional integral derivative

  10. Growing attraction of refuse-derived fuels

    SciTech Connect (OSTI)

    Singh, R.

    1981-09-08

    A review of Dr. Andrew Porteous' book, Refuse Derived Fuels is presented. The escalating price of fossil fuel, particularily oil, together with the high cost of handling and transporting refuse makes the idea of refuse-derived fuel production an attractive and economic proposition. Refuse-derived fuel production is discussed and the various manufacturing processes in the UK and the USA are described. The pyrolysis of refuse for the production of gas, oil or heat and the production of methane and ethyl alcohol or other possibilities for refuse conversion.

  11. Humic acid preparation, properties and interactions with metals lead and cadmium

    E-Print Network [OSTI]

    Coles, Cynthia

    Humic acid preparation, properties and interactions with metals lead and cadmium Cynthia A. Coles a of HAs Engineering Geology 85 (2006) 26­32 www.elsevier.com/locate/enggeo Corresponding author. Tel.: +1 in the early stages of decomposition (Yong and Mourato, 1988), is derived from lignin in woody plants (Rashid

  12. Photoelectron Spectroscopy of Anilinide and Acidity of Aniline Scott W. Wren,

    E-Print Network [OSTI]

    Lineberger, W. Carl

    ion thermochemical cycle to derive the deprotonation enthalpy of aniline at 0 K; acidH0(PhHN-H) = 1535-stabilized radical has been a subject of a large number of spectroscopic and thermochemical studies,4-10 revealing (BDE) of aniline, D0(PhHN-H). This valuable thermochemical quantity can be utilized to deduce another

  13. Poly(lactic acid) degradable plastics, coatings, and binders

    SciTech Connect (OSTI)

    Bonsignore, P.V.; Coleman, R.D.; Mudde, J.P.

    1992-01-01

    Biochemical processes to derive value from the management of high carbohydrate food wastes, such as potato starch, corn starch, and cheese whey permeate, have typically been limited to the production of either ethanol or methane. Argonne National Laboratory (ANL) believes that lactic acid presents an attractive option for an alternate fermentation end product, especially in light of lactic acids' being a viable candidate for conversion to environmentally safe poly(lactic acid) (PLA) degradable plastics, coatings, and binders. Technology is being developed at ANL to permit a more cost effective route to modified high molecular weight PLA. Preliminary data on the degradation behavior of these modified PLAs shows the retention to the inherent hydrolytic degradability of the PLA modified, however, by introduced compositional variables. A limited study was done on the hydrolytic stability of soluble oligomers of poly(L-lactic acid). Over a 34 day hold period, water-methanol solutions of Pl-LA oligomers in the 2-10 DP range retained some 75% of their original molecular weight.

  14. Poly(lactic acid) degradable plastics, coatings, and binders

    SciTech Connect (OSTI)

    Bonsignore, P.V.; Coleman, R.D.; Mudde, J.P.

    1992-05-01

    Biochemical processes to derive value from the management of high carbohydrate food wastes, such as potato starch, corn starch, and cheese whey permeate, have typically been limited to the production of either ethanol or methane. Argonne National Laboratory (ANL) believes that lactic acid presents an attractive option for an alternate fermentation end product, especially in light of lactic acids` being a viable candidate for conversion to environmentally safe poly(lactic acid) (PLA) degradable plastics, coatings, and binders. Technology is being developed at ANL to permit a more cost effective route to modified high molecular weight PLA. Preliminary data on the degradation behavior of these modified PLAs shows the retention to the inherent hydrolytic degradability of the PLA modified, however, by introduced compositional variables. A limited study was done on the hydrolytic stability of soluble oligomers of poly(L-lactic acid). Over a 34 day hold period, water-methanol solutions of Pl-LA oligomers in the 2-10 DP range retained some 75% of their original molecular weight.

  15. Acid sorption regeneration process using carbon dioxide

    DOE Patents [OSTI]

    King, C. Judson (Kensington, CA); Husson, Scott M. (Anderson, SC)

    2001-01-01

    Carboxylic acids are sorbed from aqueous feedstocks onto a solid adsorbent in the presence of carbon dioxide under pressure. The acids are freed from the sorbent phase by a suitable regeneration method, one of which is treating them with an organic alkylamine solution thus forming an alkylamine-carboxylic acid complex which thermally decomposes to the desired carboxylic acid and the alkylamine.

  16. Thermal Stability of Acetohydroxamic Acid/Nitric Acid Solutions

    SciTech Connect (OSTI)

    Rudisill, T.S.

    2002-03-13

    The transmutation of transuranic actinides and long-lived fission products in spent commercial nuclear reactor fuel has been proposed as one element of the Advanced Accelerator Applications Program. Preparation of targets for irradiation in an accelerator-driven subcritical reactor would involve dissolution of the fuel and separation of uranium, technetium, and iodine from the transuranic actinides and other fission products. The UREX solvent extraction process is being developed to reject and isolate the transuranic actinides in the acid waste stream by scrubbing with acetohydroxamic acid (AHA). To ensure that a runaway reaction will not occur between nitric acid and AHA, an analogue of hydroxyl amine, thermal stability tests were performed to identify if any processing conditions could lead to a runaway reaction.

  17. Human retroviruses and AIDS 1996. A compilation and analysis of nucleic acid and amino acid sequences

    SciTech Connect (OSTI)

    Myers, G.; Foley, B.; Korber, B.; Mellors, J.W.; Jeang, K.T.; Wain-Hobson, S.

    1997-04-01

    This compendium and the accompanying floppy diskettes are the result of an effort to compile and rapidly publish all relevant molecular data concerning the human immunodeficiency viruses (HIV) and related retroviruses. The scope of the compendium and database is best summarized by the five parts that it comprises: (1) Nuclear Acid Alignments and Sequences; (2) Amino Acid Alignments; (3) Analysis; (4) Related Sequences; and (5) Database Communications. Information within all the parts is updated throughout the year on the Web site, http://hiv-web.lanl.gov. While this publication could take the form of a review or sequence monograph, it is not so conceived. Instead, the literature from which the database is derived has simply been summarized and some elementary computational analyses have been performed upon the data. Interpretation and commentary have been avoided insofar as possible so that the reader can form his or her own judgments concerning the complex information. In addition to the general descriptions of the parts of the compendium, the user should read the individual introductions for each part.

  18. Direct synthesis of pyridine and pyrimidine derivatives

    E-Print Network [OSTI]

    Hill, Matthew D. (Matthew Dennis)

    2008-01-01

    I. Synthesis of Substituted Pyridine Derivatives via the Ruthenium-Catalyzed Cycloisomerization of 3-Azadienynes. The two-step conversion of various N-vinyl and N-aryl amides to the corresponding substituted pyridines and ...

  19. SCM Forcing Data Derived from NWP Analyses

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Jakob, Christian

    2008-01-15

    Forcing data, suitable for use with single column models (SCMs) and cloud resolving models (CRMs), have been derived from NWP analyses for the ARM (Atmospheric Radiation Measurement) Tropical Western Pacific (TWP) sites of Manus Island and Nauru.

  20. Isatin Derivatives as Inhibitors of Microtubule Assembly

    E-Print Network [OSTI]

    Beckman, Karen

    2008-09-04

    This thesis describes the rationale, design, and syntheses of derivatives of isatin (1-H-indole-2,3-dione). Isatin was identified, during a high throughput screen of 10,000 compounds, as a potential scaffold for ...

  1. Tax Credit for Forest Derived Biomass

    Broader source: Energy.gov [DOE]

    Forest-derived biomass includes tree tops, limbs, needles, leaves, and other woody debris leftover from activities such as timber harvesting, forest thinning, fire suppression, or forest health m...

  2. Confidence Measures Derived from an Acceptor HMM 

    E-Print Network [OSTI]

    Williams, Gethin; Renals, Steve

    In this paper we define a number of confidence measures derived from an acceptor HMM and evaluate their performance for the task of utterance verification using the North American Business News (NAB) and Broadcast News (BN) corpora. Results...

  3. Deriving Mathisson - Papapetrou equations from relativistic pseudomechanics

    E-Print Network [OSTI]

    R. R. Lompay

    2005-03-12

    It is shown that the equations of motion of a test point particle with spin in a given gravitational field, so called Mathisson - Papapetrou equations, can be derived from Euler - Lagrange equations of the relativistic pseudomechanics -- relativistic mechanics, which side by side uses the conventional (commuting) and Grassmannian (anticommuting) variables. In this approach the known difficulties of the Mathisson - Papapetrou equations, namely, the problem of the choice of supplementary conditions and the problem of higher derivatives are not appear.

  4. By studying the mechanisms of wax and oil production in plants and genetically manipulating their fatty acids chains, Ljerka Kunst hopes

    E-Print Network [OSTI]

    Karczmarek, Joanna

    By studying the mechanisms of wax and oil production in plants and genetically manipulating, locally- produced seed oils for industrial applications. All land plants produce a thin wax coating and altering the complex array of enzy- matic reactions involved in wax production is an integral part of plant

  5. Fatty Acid Blood Levels, Vitamin D Status, and Physical Performance and Its Relationship to Resiliency and Mood in Active Duty Soldiers 

    E-Print Network [OSTI]

    Barringer, Nicholas D

    2015-05-11

    The mental health of soldiers is a growing concern as rates of depression and suicide have increased in soldiers with recently more deaths attributed to suicide than deaths due to combat in Afghanistan in 2012. Previous ...

  6. Effect of HZE radiation and diets rich in fiber and n-3 poly unsaturated fatty acids (n-3 PUFA) on colon cancer in rats 

    E-Print Network [OSTI]

    Glagolenko, Anna Anatolievna

    2006-08-16

    weeks earlier and at the end of the study had morbidity/mortality rate 14.2% higher (P=0.0005) than non-irradiated rats. There was no significant effect of HZE radiation on colon cancer incidence. The effects of dietary fibers and oils on health state...

  7. Transcriptional modulation of hepatic lipoprotein assembly and secretion : coordinate regulation of the liver-fatty acid binding protein and microsomal triglyceride transfer protein genes

    E-Print Network [OSTI]

    Spann, Nathanael J.

    2006-01-01

    et al. 2001. Control of hepatic gluconeogenesis through thegenes involved in gluconeogenesis and mitochondrial

  8. The role of apaptosis in the suppression of lymphoproliferation which is observed in animals fed diets rich in n-3 polyunsaturated fatty acids 

    E-Print Network [OSTI]

    Shawver, Paula Ann

    2013-02-22

    oagc (( pcr cage lor onc group) m pohcarbonaic ages on siainleyi steel mesh Doors and provided free access (o commercial chnw und iap wauv I ollovnng, a ono-iicek acclnnation porn&4 ihc mico were fcd dicta wnh 4% corn orl + I H arachidonic acrd...

  9. Evaluation of bone biochemical markers and inflammatory markers in yearlings fed varying ratios of omega-6 and omega-3 polyunsaturated fatty acids 

    E-Print Network [OSTI]

    Ross, Trinette Noel

    2009-05-15

    . Thanks to my family, my parents Denny and Linda Ross, and my sisters Tracy and Buffy. I couldn?t have done it without all your love, support and confidence in me. Thanks to Dick and Kathy Goodell. None of this would have been possible without your... support. Thank you for having a vested interest in my education. And to the Griffith family: second to my own family, your encouragement and assurance was overwhelming and greatly appreciated! A special thanks to the nine yearlings on my project: Doc...

  10. Acidizing High-Temperature Carbonate Formations Using Methanesulfonic Acid 

    E-Print Network [OSTI]

    Ortega, Alexis

    2015-03-25

    Hydrochloric acid (HCl) is the most commonly used stimulation fluid for high-temperature wells drilled in carbonate reservoirs due to its high dissolving power and low cost. However, the high corrosion rate of HCl on well tubulars could make its use...

  11. Process for removal of ammonia and acid gases from contaminated waters

    DOE Patents [OSTI]

    King, C. Judson (Kensington, CA); MacKenzie, Patricia D. (Berkeley, CA)

    1985-01-01

    Contaminating basic gases, i.e., ammonia, and acid gases, e.g., carbon dioxide, are removed from process waters or waste waters in a combined extraction and stripping process. Ammonia in the form of ammonium ion is extracted by an immiscible organic phase comprising a liquid cation exchange component, especially an organic phosphoric acid derivative, and preferably di-2-ethyl hexyl phosphoric acid, dissolved in an alkyl hydrocarbon, aryl hydrocarbon, higher alcohol, oxygenated hydrocarbon, halogenated hydrocarbon, and mixtures thereof. Concurrently, the acidic gaseous contaminants are stripped from the process or waste waters by stripping with steam, air, nitrogen, or the like. The liquid cation exchange component has the ammonia stripped therefrom by heating, and the component may be recycled to extract additional amounts of ammonia.

  12. Process for removal of ammonia and acid gases from contaminated waters

    DOE Patents [OSTI]

    King, C.J.; Mackenzie, P.D.

    1982-09-03

    Contaminating basic gases, i.e., ammonia and acid gases, e.g., carbon dioxide, are removed from process waters or waste waters in a combined extraction and stripping process. Ammonia in the form of ammonium ion is extracted by an immiscible organic phase comprising a liquid cation exchange component, especially an organic phosphoric acid derivative, and preferably di-2-ethyl hexyl phosphoric acid, dissolved in an alkyl hydrocarbon, aryl hydrocarbon, higher alcohol, oxygenated hydrocarbon, halogenated hydrocarbon, and mixtures thereof. Concurrently, the acidic gaseous contaminants are stripped from the process or waste waters by stripping with stream, air, nitrogen, or the like. The liquid cation exchange component has the ammonia stripped therefrom by heating, and the component may be recycled to extract additional amounts of ammonia.

  13. An assessment of acid fog

    SciTech Connect (OSTI)

    Lipfert, F.W.

    1992-12-31

    Airborne particles have long been associated with adverse effects on public health, begin with the notorious air pollution disasters of several decades ago. Although H{sub 2}SO{sub 4} was identified early on as a potential causal factors during these episodes (in part because of concern for potential health effects of particle acidity per se has intensified only recently. Most of the recent aerometric research in the US on acid fog has focused on the ability of clouds and fog to deliver acidity to vegetation and ecosystems. Strong acids are characterized chemically by their pH or H{sup +} concentration. For fog, concentrations are referred to the droplet liquid content; for other (i.e., ``clear air``) aerosols, to the volume of air sampled. A useful measure of the relationship between aerosol and fog is obtained by comparing their mass concentrations on the basis of the same volume of air, by multiplying fogwater concentrations by liquid water content (LWC). This paper reviews fog measurement capability, physical properties and chemistry, and presents a simple urban airshed model which is used to simulate the evolution of fog and aerosol concentrations under urban stagnation conditions.

  14. Process for forming sulfuric acid

    DOE Patents [OSTI]

    Lu, Wen-Tong P. (Upper St. Clair, PA)

    1981-01-01

    An improved electrode is disclosed for the anode in a sulfur cycle hydrogen generation process where sulfur dioxie is oxidized to form sulfuric acid at the anode. The active compound in the electrode is palladium, palladium oxide, an alloy of palladium, or a mixture thereof. The active compound may be deposited on a porous, stable, conductive substrate.

  15. Second derivatives for approximate spin projection methods

    SciTech Connect (OSTI)

    Thompson, Lee M.; Hratchian, Hrant P.

    2015-02-07

    The use of broken-symmetry electronic structure methods is required in order to obtain correct behavior of electronically strained open-shell systems, such as transition states, biradicals, and transition metals. This approach often has issues with spin contamination, which can lead to significant errors in predicted energies, geometries, and properties. Approximate projection schemes are able to correct for spin contamination and can often yield improved results. To fully make use of these methods and to carry out exploration of the potential energy surface, it is desirable to develop an efficient second energy derivative theory. In this paper, we formulate the analytical second derivatives for the Yamaguchi approximate projection scheme, building on recent work that has yielded an efficient implementation of the analytical first derivatives.

  16. Quadratic forms and Clifford algebras on derived stacks

    E-Print Network [OSTI]

    Vezzosi, Gabriele

    Quadratic forms and Clifford algebras on derived stacks Gabriele Vezzosi Institut de Math structures in derived algebraic geometry. We define derived n-shifted quadratic complexes, over derived affine stacks and over general derived stacks, and give several examples of those. We define

  17. High ethanol producing derivatives of Thermoanaerobacter ethanolicus

    DOE Patents [OSTI]

    Ljungdahl, Lars G. (Athens, GA); Carriera, Laura H. (Athens, GA)

    1983-01-01

    Derivatives of the newly discovered microorganism Thermoanaerobacter ethanolicus which under anaerobic and thermophilic conditions continuously ferment substrates such as starch, cellobiose, glucose, xylose and other sugars to produce recoverable amounts of ethanol solving the problem of fermentations yielding low concentrations of ethanol using the parent strain of the microorganism Thermoanaerobacter ethanolicus are disclosed. These new derivatives are ethanol tolerant up to 10% (v/v) ethanol during fermentation. The process includes the use of an aqueous fermentation medium, containing the substrate at a substrate concentration greater than 1% (w/v).

  18. High ethanol producing derivatives of Thermoanaerobacter ethanolicus

    DOE Patents [OSTI]

    Ljungdahl, L.G.; Carriera, L.H.

    1983-05-24

    Derivatives of the newly discovered microorganism Thermoanaerobacter ethanolicus which under anaerobic and thermophilic conditions continuously ferment substrates such as starch, cellobiose, glucose, xylose and other sugars to produce recoverable amounts of ethanol solving the problem of fermentations yielding low concentrations of ethanol using the parent strain of the microorganism Thermoanaerobacter ethanolicus are disclosed. These new derivatives are ethanol tolerant up to 10% (v/v) ethanol during fermentation. The process includes the use of an aqueous fermentation medium, containing the substrate at a substrate concentration greater than 1% (w/v).

  19. Variables affecting the yields of fatty esters from transesterified vegetable oils

    SciTech Connect (OSTI)

    Freedman, B.; Pryde, E.H.; Mounts, T.L.

    1984-10-01

    Transesterification reaction variables that affect yield and purity of the product esters from cottonseed, peanut, soybean and sunflower oils include molar ratio of alcohol to vegetable oil, type of catalyst (alkaline vs acidic), temperature and degree of refinement of the vegetable oil. With alkaline catalysts (either sodium hydroxide or methoxide), temperatures of 60 degrees C or higher, molar ratios of at least 6 to 1 and with fully refined oils, conversion to methyl, ethyl and butyl esters was essentially complete in 1 hr. At moderate temperatures (32 degrees C), vegetable oils were 99% transesterified in ca. 4 hr with an alkaline catalyst. Transesterification by acid catalysis was much slower than by alkali catalysis. Although the crude oils could be transesterified, ester yields were reduced because of gums and extraneous material present in the crude oils. 30 references.

  20. Barriers to growth in the US real estate derivatives market

    E-Print Network [OSTI]

    Venter, Jani

    2007-01-01

    Commercial real estate is an important asset class but it does not yet have a well-developed derivatives market in the United States. A derivative is a contract that derives its value from an underlying index or asset. ...

  1. SEP Request for Approval Form 2 - Other Derived Energy Sources...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    2 - Other Derived Energy Sources SEP Request for Approval Form 2 - Other Derived Energy Sources SEP-Request-for-Approval-Form-2Other-Derived-Energy-Sources.docx More Documents &...

  2. Brain Derived Vision Algorithm on High Performance Architectures

    E-Print Network [OSTI]

    2009-01-01

    Granger, R. : Novel brain-derived algorithms scale linearly37:345–369 17. Brain derived vision on IBM CELL: http://10.1007/s10766-009-0106-9 Brain Derived Vision Algorithm on

  3. Development/Plasticity/Repair Dorsally and Ventrally Derived Oligodendrocytes Have

    E-Print Network [OSTI]

    Richardson, William D.

    Development/Plasticity/Repair Dorsally and Ventrally Derived Oligodendrocytes Have Similar derived oligodendrocyte (OL) lineage cells have different properties. We generated a dual reporter mouse line to color code ventrally and dorsally derived OLPs (vOLPs and d

  4. Constraining Higher Derivative Supergravity with Scattering Amplitudes

    E-Print Network [OSTI]

    Yifan Wang; Xi Yin

    2015-03-05

    We study supersymmetry constraints on higher derivative deformations of type IIB supergravity by consideration of superamplitudes. Combining constraints of on-shell supervertices and basic results from string perturbation theory, we give a simple argument for the non-renormalization theorem of Green and Sethi, and some of its generalizations.

  5. Derivation of a Stochastic Neutron Transport Equation

    E-Print Network [OSTI]

    Edward J. Allen

    2010-04-14

    Stochastic difference equations and a stochastic partial differential equation (SPDE) are simultaneously derived for the time-dependent neutron angular density in a general three-dimensional medium where the neutron angular density is a function of position, direction, energy, and time. Special cases of the equations are given such as transport in one-dimensional plane geometry with isotropic scattering and transport in a homogeneous medium. The stochastic equations are derived from basic principles, i.e., from the changes that occur in a small time interval. Stochastic difference equations of the neutron angular density are constructed, taking into account the inherent randomness in scatters, absorptions, and source neutrons. As the time interval decreases, the stochastic difference equations lead to a system of Ito stochastic differential equations (SDEs). As the energy, direction, and position intervals decrease, an SPDE is derived for the neutron angular density. Comparisons between numerical solutions of the stochastic difference equations and independently formulated Monte Carlo calculations support the accuracy of the derivations.

  6. Equity and Equity Index Derivatives Trading Strategies

    E-Print Network [OSTI]

    Ciocan-Fontanine, Ionut

    Indexes 20 Theoretical (Fair) Value 21 Basis 22 Cost of Carry = Basis Equity Index Futures Strategies 23e u r e x Equity and Equity Index Derivatives Trading Strategies e u r e x #12;Please Note The definitions of "basis" and "cost of carry" have been changed in this version of the brochure. In previous

  7. Higher Derivative D-brane Couplings 

    E-Print Network [OSTI]

    Guo, Guangyu

    2012-10-19

    supersymmetry. In the third part, we obtain the higher derivative D-brane action by using both linearized T-duality and string disc amplitude computation. We evaluate disc amplitude of one R-R field C^(p-3) and two NS-NS fields in the presence of a single Dp...

  8. Derivation of a poroelastic flexural shell model

    E-Print Network [OSTI]

    Mikelic, Andro

    2015-01-01

    In this paper we investigate the limit behavior of the solution to quasi-static Biot's equations in thin poroelastic flexural shells as the thickness of the shell tends to zero and extend the results obtained for the poroelastic plate by Marciniak-Czochra and Mikeli\\'c. We choose Terzaghi's time corresponding to the shell thickness and obtain the strong convergence of the three-dimensional solid displacement, fluid pressure and total poroelastic stress to the solution of the new class of shell equations. The derived bending equation is coupled with the pressure equation and it contains the bending moment due to the variation in pore pressure across the shell thickness. The effective pressure equation is parabolic only in the normal direction. As additional terms it contains the time derivative of the middle-surface flexural strain. Derivation of the model presents an extension of the results on the derivation of classical linear elastic shells by Ciarlet and collaborators to the poroelastic shells case. The n...

  9. Biofuels and bio-products derived from

    E-Print Network [OSTI]

    Ginzel, Matthew

    NEED Biofuels and bio- products derived from lignocellulosic biomass (plant materials) are part improve the energy and carbon efficiencies of biofuels production from a barrel of biomass using chemical and thermal catalytic mechanisms. The Center for Direct Catalytic Conversion of Biomass to Biofuels IMPACT

  10. Wave function derivation of the JIMWLK equation

    E-Print Network [OSTI]

    Alexey V. Popov

    2008-12-16

    Using the stationary lightcone perturbation theory, we propose the complete and careful derivation the JIMWLK equation. We show that the rigorous treatment requires the knowledge of a boosted wave function with second order accuracy. Previous wave function approaches are incomplete and implicitly used the time ordered perturbation theory, which requires a usage of an external target field.

  11. High speed point derivative microseismic detector

    DOE Patents [OSTI]

    Uhl, J.E.; Warpinski, N.R.; Whetten, E.B.

    1998-06-30

    A high speed microseismic event detector constructed in accordance with the present invention uses a point derivative comb to quickly and accurately detect microseismic events. Compressional and shear waves impinging upon microseismic receiver stations disposed to collect waves are converted into digital data and analyzed using a point derivative comb including assurance of quiet periods prior to declaration of microseismic events. If a sufficient number of quiet periods have passed, the square of a two point derivative of the incoming digital signal is compared to a trip level threshold exceeding the determined noise level to declare a valid trial event. The squaring of the derivative emphasizes the differences between noise and signal, and the valid event is preferably declared when the trip threshold has been exceeded over a temporal comb width to realize a comb over a given time period. Once a trial event has been declared, the event is verified through a spatial comb, which applies the temporal event comb to additional stations. The detector according to the present invention quickly and accurately detects initial compressional waves indicative of a microseismic event which typically exceed the ambient cultural noise level by a small amount, and distinguishes the waves from subsequent larger amplitude shear waves. 9 figs.

  12. High speed point derivative microseismic detector

    DOE Patents [OSTI]

    Uhl, James Eugene (Albuquerque, NM); Warpinski, Norman Raymond (Albuquerque, NM); Whetten, Ernest Blayne (Albuquerque, NM)

    1998-01-01

    A high speed microseismic event detector constructed in accordance with the present invention uses a point derivative comb to quickly and accurately detect microseismic events. Compressional and shear waves impinging upon microseismic receiver stations disposed to collect waves are converted into digital data and analyzed using a point derivative comb including assurance of quiet periods prior to declaration of microseismic events. If a sufficient number of quiet periods have passed, the square of a two point derivative of the incoming digital signal is compared to a trip level threshold exceeding the determined noise level to declare a valid trial event. The squaring of the derivative emphasizes the differences between noise and signal, and the valid event is preferably declared when the trip threshold has been exceeded over a temporal comb width to realize a comb over a given time period. Once a trial event has been declared, the event is verified through a spatial comb, which applies the temporal event comb to additional stations. The detector according to the present invention quickly and accurately detects initial compressional waves indicative of a microseismic event which typically exceed the ambient cultural noise level by a small amount, and distinguishes the waves from subsequent larger amplitude shear waves.

  13. Derivative of Map of Banach algebra

    E-Print Network [OSTI]

    Aleks Kleyn

    2015-05-14

    Let $A$ be Banach algebra over commutative ring $D$. The map $f:A\\rightarrow A\\ $ is called differentiable in the Gateaux sense, if $$f(x+a)-f(x)=\\partial f(x)\\circ a+o(a)$$ where the Gateaux derivative $\\partial f(x)$ of map $f$ is linear map of increment $a$ and $o$ is such continuous map that $$ \\lim_{a\\rightarrow 0}\\frac{|o(a)|}{|a|}=0 $$ Assuming that we defined the Gateaux derivative $\\partial^{n-1} f(x)$ of order $n-1$, we define $$ \\partial^n f(x)\\circ(a_1\\otimes...\\otimes a_n) =\\partial(\\partial^{n-1} f(x)\\circ(a_1\\otimes...\\otimes a_{n-1}))\\circ a_n $$ the Gateaux derivative of order $n$ of map $f$. Since the map $f(x)$ has all derivatives, then the map $f(x)$ has Taylor series expansion $$ f(x)=\\sum_{n=0}^{\\infty}(n!)^{-1}\\partial^n f(x_0)\\circ(x-x_0)^n $$

  14. Replica amplification of nucleic acid arrays

    DOE Patents [OSTI]

    Church, George M. (Brookline, MA)

    2002-01-01

    A method of producing a plurality of a nucleic acid array, comprising, in order, the steps of amplifying in situ nucleic acid molecules of a first randomly-patterned, immobilized nucleic acid array comprising a heterogeneous pool of nucleic acid molecules affixed to a support, transferring at least a subset of the nucleic acid molecules produced by such amplifying to a second support, and affixing the subset so transferred to the second support to form a second randomly-patterned, immobilized nucleic acid array, wherein the nucleic acid molecules of the second array occupy positions that correspond to those of the nucleic acid molecules from which they were amplified on the first array, so that the first array serves as a template to produce a plurality, is disclosed.

  15. Modeling of Acid Fracturing in Carbonate Reservoirs 

    E-Print Network [OSTI]

    Al Jawad, Murtada s

    2014-06-05

    The acid fracturing process is a thermal, hydraulic, mechanical, and geochemical (THMG)-coupled phenomena in which the behavior of these variables are interrelated. To model the flow behavior of an acid into a fracture, mass and momentum balance...

  16. PREDICTING TEMPERATURE BEHAVIOR IN CARBONATE ACIDIZING TREATMENTS 

    E-Print Network [OSTI]

    Tan, Xuehao

    2010-01-16

    ........................................................................................ 4 1.4 Objective and Procedures .................................................................. 4 1.5 Outline .............................................................................................. 5 2. ACID INJECTION PROBLEM.......................................................... 10 2.4 Wormhole Growth Model.................................................................. 11 2.5 Modified Volumetric Model .............................................................. 14 2.6 Solution of Acid Injection Problem...

  17. Recovery of mercury from acid waste residues

    DOE Patents [OSTI]

    Greenhalgh, W.O.

    1987-02-27

    Mercury can be recovered from nitric acid-containing fluids by reacting the fluid with aluminum metal to produce mercury metal, and thence quenching the reactivity of the nitric acid prior to nitration of the mercury metal. 1 fig.

  18. Fuel cell electrolyte membrane with acidic polymer

    DOE Patents [OSTI]

    Hamrock, Steven J. (Stillwater, MN); Larson, James M. (Saint Paul, MN); Pham, Phat T. (Little Canada, MN); Frey, Matthew H. (Cottage Grove, MN); Haugen, Gregory M. (Edina, MN); Lamanna, William M. (Stillwater, MN)

    2009-04-14

    An electrolyte membrane is formed by an acidic polymer and a low-volatility acid that is fluorinated, substantially free of basic groups, and is either oligomeric or non-polymeric.

  19. Synthesis of Methyl Methacrylate from Coal-Derived Syngas

    SciTech Connect (OSTI)

    Gerald N. Choi; James J. Spivey; Jospeh R. Zoeller; Makarand R. Gogate; Richard D. Colberg; Samuel S. Tam

    1998-04-17

    Research Triangle Institute (RTI), Eastman Chemical Company, and Bechtel collectively are developing a novel three-step process for the synthesis of methyl methacrylate (MMA) from coal-derived syngas that consists of the steps of synthesis of a propionate, its condensation with formaldehyde to form methacrylic acid (MAA), and esterification of MAA with methanol to produce MMA. RTI has completed the research on the three-step methanol-based route to MMA. Under an extension to the original contract, RTI is currently evaluating a new DME-based process for MMA. The key research need for DME route is to develop catalysts for DME partial oxidation reactions and DME condensation reactions. Over the last month, RTI has finalized the design of a fixed-bed microreactor system for DME partial oxidation reactions. RTI incorporated some design changes to the feed blending system, so as to be able to blend varying proportions of DME and oxygen. RTI has also examined the flammability limits of DME-air mixtures. Since the lower flammability limit of DME in air is 3.6 volume percent, RTI will use a nominal feed composition of 1.6 percent in air, which is less than half the lower explosion limit for DME-air mixtures. This nominal feed composition is thus considered operationally safe, for DME partial oxidation reactions. RTI is also currently developing an analytical system for DME partial oxidation reaction system.

  20. Synthesis of methyl methacrylate from coal-derived syngas: Quarterly report,, October 1-December 31, 1997

    SciTech Connect (OSTI)

    1998-09-01

    Research Triangle Institute (RTI), Eastman Chemical Company, and Bechtel collectively are developing a novel process for the synthesis of methyl methacrylate (MMA) from coal-derived syngas that consists of three steps of synthesis of a propionate, its condensation with formaldehyde, and esterification of resulting methacrylic acid (MAA) with methanol to produce MMA. Over the last quarter, Eastman developed two new processes which have resulted in two new invention reports. One process deals with carbonylation of benzyl ether which represents a model for coal liquefaction and the second focuses on the acceleration of carbonylation rates for propionic acid synthesis, via use of polar aprotic solvents. These two inventions are major improvements in the novel Mo-catalyzed homogeneous process for propionic acid synthesis technology, developed by Eastman. Over the last quarter, RTI completed three reaction cycles and two regeneration cycles as a part of long-term reaction regeneration cycle study on a 10% Nb{sub 2}O{sub 5}/Si0{sub 2} catalyst, for vapor phase condensation reaction of formaldehyde with propionic acid.

  1. Chemical Additive Selection in Matrix Acidizing 

    E-Print Network [OSTI]

    Weidner, Jason 1981-

    2011-05-09

    critical detail of weak acid chemistry. One concern when using any acid in oilfield operations is the corrosion of well tubulars. Thus operators often choose to pump corrosion inhibitor, a chemical additive electrostatically attracted... to the negative charge of the well casing or production tubing, to decrease the rate at which the acid accesses well tubular surfaces (Crowe and Minor 1985). A typical working concentration of corrosion inhibitor is 1-2 wt% of injected acid (Smith et al. 1978...

  2. Production of high molecular weight polylactic acid

    DOE Patents [OSTI]

    Bonsignore, Patrick V. (Joilet, IL)

    1995-01-01

    A degradable high molecular weight poly(lactic acid). A poly(lactic acid) has a terminal end group of one of carboxyl or hydroxyl groups with low molecular weight poly(lactic acid) units coupled with linking agents of di-isocyanates, bis-epoxides, bis-oxazolines and bis-ortho esters. The resulting high molecular weight poly(lactic acid) can be used for applications taking advantage of the improved physical properties.

  3. Production of high molecular weight polylactic acid

    DOE Patents [OSTI]

    Bonsignore, P.V.

    1995-11-28

    A degradable high molecular weight poly(lactic acid) is described. The poly(lactic acid) has a terminal end group of one of carboxyl or hydroxyl groups with low molecular weight poly(lactic acid) units coupled with linking agents of di-isocyanates, bis-epoxides, bis-oxazolines and bis-ortho esters. The resulting high molecular weight poly(lactic acid) can be used for applications taking advantage of the improved physical properties.

  4. Metabolically active eukaryotic communities in extremely acidic mine drainage

    E-Print Network [OSTI]

    Baker, Brett J; Lutz, M A; Dawson, S C; Bond, P L; Banfield, J F

    2004-01-01

    Microbial communities in acid mine drainage. FEMS Microbiol.Biogeochem- istry of acid mine drainage at Iron Mountain,in an extreme acid mine drainage environment. Appl. Environ.

  5. Detailed Characterization of Lubricant-Derived Ash-Related Species...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Characterization of Lubricant-Derived Ash-Related Species in Diesel Exhaust and Aftertreatment Systems Detailed Characterization of Lubricant-Derived Ash-Related Species in Diesel...

  6. Derivative-free optimization for parameter estimation in computational...

    Office of Scientific and Technical Information (OSTI)

    Derivative-free optimization for parameter estimation in computational nuclear physics Citation Details In-Document Search Title: Derivative-free optimization for parameter...

  7. Low-Emissions Burner Technology using Biomass-Derived Liquid...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Low-Emissions Burner Technology using Biomass-Derived Liquid Fuels Low-Emissions Burner Technology using Biomass-Derived Liquid Fuels This factsheet describes a project that...

  8. The Impact of Using Derived Fuel Consumption Maps to Predict...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    The Impact of Using Derived Fuel Consumption Maps to Predict Fuel Consumption The Impact of Using Derived Fuel Consumption Maps to Predict Fuel Consumption Poster presented at the...

  9. BILIWG Meeting: High Pressure Steam Reforming of Bio-Derived...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    High Pressure Steam Reforming of Bio-Derived Liquids (Presentation) BILIWG Meeting: High Pressure Steam Reforming of Bio-Derived Liquids (Presentation) Presented at the 2007...

  10. Bio-Derived Liquids to Hydrogen Distributed Reforming Targets...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Targets (Presentation) Bio-Derived Liquids to Hydrogen Distributed Reforming Targets (Presentation) Presented at the 2007 Bio-Derived Liquids to Hydrogen Distributed Reforming...

  11. Unnatural reactive amino acid genetic code additions

    SciTech Connect (OSTI)

    Deiters, Alexander; Cropp, T. Ashton; Chin, Jason W.; Anderson, J. Christopher; Schultz, Peter G.

    2014-08-26

    This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in eukaryotic cells. The components include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, orthogonal pairs of tRNAs/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in eukaryotic cells are also provided.

  12. Unnatural reactive amino acid genetic code additions

    DOE Patents [OSTI]

    Deiters, Alexander (La Jolla, CA); Cropp, T. Ashton (San Diego, CA); Chin, Jason W. (Cambridge, GB); Anderson, J. Christopher (San Francisco, CA); Schultz, Peter G. (La Jolla, CA)

    2011-02-15

    This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in eukaryotic cells. The components include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, orthogonal pairs of tRNAs/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in eukaryotic cells are also provided.

  13. Unnatural reactive amino acid genetic code additions

    DOE Patents [OSTI]

    Deiters, Alexander (La Jolla, CA); Cropp, T. Ashton (Bethesda, MD); Chin, Jason W. (Cambridge, GB); Anderson, J. Christopher (San Francisco, CA); Schultz, Peter G. (La Jolla, CA)

    2011-08-09

    This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in eukaryotic cells. The components include orthogonal tRNAs, orthogonal aminoacyl-tRNAsyn-thetases, pairs of tRNAs/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in eukaryotic cells are also provided.

  14. Unnatural reactive amino acid genetic code additions

    DOE Patents [OSTI]

    Deiters, Alexander; Cropp, Ashton T; Chin, Jason W; Anderson, Christopher J; Schultz, Peter G

    2013-05-21

    This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in eukaryotic cells. The components include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, pairs of tRNAs/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in eukaryotic cells are also provided.

  15. Synthesis of an acid addition salt of delta-aminolevulinic acid from 5-bromo levulinic acid esters

    DOE Patents [OSTI]

    Moens, L.

    1999-05-25

    A process is disclosed for preparing an acid addition salt of delta-aminolevulinic acid comprising. The process involves dissolving a lower alkyl 5-bromolevulinate and an alkali metal diformylamide in an organic solvent selected from the group consisting of acetonitrile, methanol, tetrahydrofuran, 2-methyltetrahydrofuran and methylformate or mixtures to form a suspension of an alkyl 5-(N,N-diformylamino) levulinate ester; and hydrolyzing the alkyl 5-(N,N-diformylamino) levulinate with an inorganic acid to form an acid addition salt of delta-amino levulinic acid.

  16. Synthesis of an acid addition salt of delta-aminolevulinic acid from 5-bromo levulinic acid esters

    DOE Patents [OSTI]

    Moens, Luc (Lakewood, CO)

    1999-01-01

    A process of preparing an acid addition salt of delta-aminolevulinic acid comprising: dissolving a lower alkyl 5-bromolevulinate and an alkali metal diformylamide in an organic solvent selected from the group consisting of acetonitrile, methanol, tetrahydrofuran, 2-methyltetrahydrofuran and methylformate or mixtures thereof to form a suspension of an alkyl 5-(N,N-diformylamino) levulinate ester; and hydrolyzing said alkyl 5-(N,N-diformylamino) levulinate with an inorganic acid to form an acid addition salt of delta-amino levulinic acid.

  17. Thermal Stability Of Formohydroxamic Acid

    SciTech Connect (OSTI)

    Fondeur, F. F.; Rudisill, T. S.

    2011-10-21

    The thermal stability of formohydroxamic acid (FHA) was evaluated to address the potential for exothermic decomposition during storage and its use in the uranium extraction process. Accelerating rate calorimetry showed rapid decomposition at a temperature above 65 {degree}?C; although, the rate of pressure rise was greater than two orders of magnitude less than the lower bound for materials which have no explosive properties with respect to transportation. FHA solutions in water and nitric acid did not reach runaway conditions until 150 {degree}?C. Analysis by differential scanning calorimetry showed that FHA melted at 67 {degree}?C and thermally decomposed at 90 {degree}?C with an enthalpy of -1924 J/g. The energics of the FHA thermal decomposition are comparable to those measured for aqueous solutions of hydroxylamine nitrate. Solid FHA should be stored in a location where the temperature does not exceed 20-25 {degree}?C. As a best practice, the solid material should be stored in a climate-controlled environment such as a refrigerator or freezer. FHA solutions in water are not susceptible to degradation by acid hydrolysis and are the preferred way to handle FHA prior to use.

  18. Cyclohexyl-triethylenetetraamine hexacetic acid

    DOE Patents [OSTI]

    Mease, Ronnie C. (Coram, NY); Srivastava, Suresh C. (Setauket, NY); Gestin, Jean-Francois (Oudon, FR)

    1992-01-01

    The present invention relates to new rigid chelating structures, to methods for preparing these materials, and to their use in preparing radiometal labeled immunoconjugates. These new chelates include cyclohexyl EDTA monohydride, the trans forms of cyclohexyl DTPA and TTHA, and derivatives of these cyclohexyl polyaminocarboxylate materials.

  19. Hydrochloric Acid-Catalyzed Levulinic Acid Formation from Cellulose: Data and Kinetic

    E-Print Network [OSTI]

    California at Riverside, University of

    Hydrochloric Acid-Catalyzed Levulinic Acid Formation from Cellulose: Data and Kinetic Model.com). In this study, the kinetics of the acid catalyzed hydrolysis of microcrystalline cellulose (Avicel PH101 of conditions: 160­200 C, hydrochloric acid concentrations of 0.309­0.927 M (11.3­33.8 g/l), cellulose

  20. Acid Diversion in Carbonate Reservoirs Using Polymer-Based In-Situ Gelled Acids 

    E-Print Network [OSTI]

    Gomaa, Ahmed Mohamed Mohamed

    2012-07-16

    and determine factors that impact its performance. Lab test of polymer-based in-situ gelled acids reveal that polymer and other additives separate out of the acid when these acids are prepared in high salinity water. In coreflood tests, in-situ gelled acid...

  1. Challenges in Catalytic Manufacture of Renewable Pyrrolidinones from Fermentation Derived Succinate

    SciTech Connect (OSTI)

    White, James F.; Holladay, Johnathan E.; Zacher, Alan H.; Frye, John G.; Werpy, Todd A.

    2014-09-05

    Fermentation derived succinic acid ammonium salt is an ideal precursor for manufacture of renewable N-methyl pyrrolidinone (NMP) or 2-pyrrolidinone (2P) via heterogeneous catalysis. However, there are many challenges to making this a practical reality. Chief among the challenges is avoiding catalyst poisoning by fermentation by- and co-products. Battelle / Pacific Northwest National Laboratory (PNNL) have developed an economically effective technology strategy for this purpose. The technology is a combination of purely thermal processing, followed by simple catalytic hydrogenation that together avoids catalyst poisoning from fermentation impurities and provides high selectivity and yields of NMP or 2P.

  2. Method for sequencing nucleic acid molecules

    DOE Patents [OSTI]

    Korlach, Jonas; Webb, Watt W.; Levene, Michael; Turner, Stephen; Craighead, Harold G.; Foquet, Mathieu

    2006-06-06

    The present invention is directed to a method of sequencing a target nucleic acid molecule having a plurality of bases. In its principle, the temporal order of base additions during the polymerization reaction is measured on a molecule of nucleic acid, i.e. the activity of a nucleic acid polymerizing enzyme on the template nucleic acid molecule to be sequenced is followed in real time. The sequence is deduced by identifying which base is being incorporated into the growing complementary strand of the target nucleic acid by the catalytic activity of the nucleic acid polymerizing enzyme at each step in the sequence of base additions. A polymerase on the target nucleic acid molecule complex is provided in a position suitable to move along the target nucleic acid molecule and extend the oligonucleotide primer at an active site. A plurality of labelled types of nucleotide analogs are provided proximate to the active site, with each distinguishable type of nucleotide analog being complementary to a different nucleotide in the target nucleic acid sequence. The growing nucleic acid strand is extended by using the polymerase to add a nucleotide analog to the nucleic acid strand at the active site, where the nucleotide analog being added is complementary to the nucleotide of the target nucleic acid at the active site. The nucleotide analog added to the oligonucleotide primer as a result of the polymerizing step is identified. The steps of providing labelled nucleotide analogs, polymerizing the growing nucleic acid strand, and identifying the added nucleotide analog are repeated so that the nucleic acid strand is further extended and the sequence of the target nucleic acid is determined.

  3. Method for sequencing nucleic acid molecules

    DOE Patents [OSTI]

    Korlach, Jonas; Webb, Watt W.; Levene, Michael; Turner, Stephen; Craighead, Harold G.; Foquet, Mathieu

    2006-05-30

    The present invention is directed to a method of sequencing a target nucleic acid molecule having a plurality of bases. In its principle, the temporal order of base additions during the polymerization reaction is measured on a molecule of nucleic acid, i.e. the activity of a nucleic acid polymerizing enzyme on the template nucleic acid molecule to be sequenced is followed in real time. The sequence is deduced by identifying which base is being incorporated into the growing complementary strand of the target nucleic acid by the catalytic activity of the nucleic acid polymerizing enzyme at each step in the sequence of base additions. A polymerase on the target nucleic acid molecule complex is provided in a position suitable to move along the target nucleic acid molecule and extend the oligonucleotide primer at an active site. A plurality of labelled types of nucleotide analogs are provided proximate to the active site, with each distinguishable type of nucleotide analog being complementary to a different nucleotide in the target nucleic acid sequence. The growing nucleic acid strand is extended by using the polymerase to add a nucleotide analog to the nucleic acid strand at the active site, where the nucleotide analog being added is complementary to the nucleotide of the target nucleic acid at the active site. The nucleotide analog added to the oligonucleotide primer as a result of the polymerizing step is identified. The steps of providing labelled nucleotide analogs, polymerizing the growing nucleic acid strand, and identifying the added nucleotide analog are repeated so that the nucleic acid strand is further extended and the sequence of the target nucleic acid is determined.

  4. Low Temperature Autoignition of C8H16O2 Ethyl and Methyl Esters in a Motored Engine

    Office of Energy Efficiency and Renewable Energy (EERE)

    The alkyl chain of fatty acid esters experience the typical paraffin-like low temperature oxidation sequence; the alkyl chain length of fatty acid esters has a crucial impact on the ignition behavior of fatty acid esters

  5. Solid-state actinide acid phosphites from phosphorous acid melts

    SciTech Connect (OSTI)

    Oh, George N. [Department of Civil and Environmental Engineering and Earth Sciences, University of Notre Dame, 156 Fitzpatrick Hall, Notre Dame, IN 46556 (United States); Burns, Peter C., E-mail: pburns@nd.edu [Department of Civil and Environmental Engineering and Earth Sciences, University of Notre Dame, 156 Fitzpatrick Hall, Notre Dame, IN 46556 (United States); Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556 (United States)

    2014-07-01

    The reaction of UO{sub 3} and H{sub 3}PO{sub 3} at 100 °C and subsequent reaction with dimethylformamide (DMF) produces crystals of the compound (NH{sub 2}(CH{sub 3}){sub 2})[UO{sub 2}(HPO{sub 2}OH)(HPO{sub 3})]. This compound crystallizes in space group P2{sub 1}/n and consists of layers of uranyl pentagonal bipyramids that share equatorial vertices with phosphite units, separated by dimethylammonium. In contrast, the reaction of phosphorous acid and actinide oxides at 210 °C produces a viscous syrup. Subsequent dilution in solvents and use of standard solution-state methods results in the crystallization of two polymorphs of the actinide acid phosphites An(HPO{sub 2}OH){sub 4} (An=U, Th) and of the mixed acid phosphite–phosphite U(HPO{sub 3})(HPO{sub 2}OH){sub 2}(H{sub 2}O)·2(H{sub 2}O). ?- and ?-An(HPO{sub 2}OH){sub 4} crystallize in space groups C2/c and P2{sub 1}/n, respectively, and comprise a three-dimensional network of An{sup 4+} cations in square antiprismatic coordination corner-sharing with protonated phosphite units, whereas U(HPO{sub 3})(HPO{sub 2}OH){sub 2}(H{sub 2}O){sub 2}·(H{sub 2}O) crystallizes in a layered structure in space group Pbca that is composed of An{sup 4+} cations in square antiprismatic coordination corner-sharing with protonated phosphites and water ligands. We discuss our findings in using solid inorganic reagents to produce a solution-workable precursor from which solid-state compounds can be crystallized. - Graphical abstract: Reaction of UO{sub 3} and H{sub 3}PO{sub 3} at 100 °C and subsequent reaction with DMF produces crystals of (NH{sub 2}(CH{sub 3}){sub 2})[UO{sub 2}(HPO{sub 2}OH)(HPO{sub 3})] with a layered structure. Reaction of phosphorous acid and actinide oxides at 210 °C produces a viscous syrup and further solution-state reactions result in the crystallization of the actinide acid phosphites An(HPO{sub 2}OH){sub 4} (An=U, Th), with a three-dimensional network structure, and the mixed acid phosphite–phosphite U(HPO{sub 3})(HPO{sub 2}OH){sub 2}(H{sub 2}O){sub 2}·(H{sub 2}O) with a layered structure. - Highlights: • U(VI), U(IV) and Th(IV) phosphites were synthesized by solution-state methods. • A new uranyl phosphite structure is based upon uranyl phosphite anionic sheets. • New U and Th phosphites have framework structures.

  6. Derivation of evolutionary payoffs from observable behavior

    E-Print Network [OSTI]

    Feigel, Alexander; Engel, Assaf

    2008-01-01

    Interpretation of animal behavior, especially as cooperative or selfish, is a challenge for evolutionary theory. Strategy of a competition should follow from corresponding Darwinian payoffs for the available behavioral options. The payoffs and decision making processes, however, are difficult to observe and quantify. Here we present a general method for the derivation of evolutionary payoffs from observable statistics of interactions. The method is applied to combat of male bowl and doily spiders, to predator inspection by sticklebacks and to territorial defense by lions, demonstrating animal behavior as a new type of game theoretical equilibrium. Games animals play may be derived unequivocally from their observable behavior, the reconstruction, however, can be subjected to fundamental limitations due to our inability to observe all information exchange mechanisms (communication).

  7. A derivative standard for polarimeter calibration

    SciTech Connect (OSTI)

    Mulhollan, G.; Clendenin, J.; Saez, P. [and others

    1996-10-01

    A long-standing problem in polarized electron physics is the lack of a traceable standard for calibrating electron spin polarimeters. While several polarimeters are absolutely calibrated to better than 2%, the typical instrument has an inherent accuracy no better than 10%. This variability among polarimeters makes it difficult to compare advances in polarized electron sources between laboratories. The authors have undertaken an effort to establish 100 nm thick molecular beam epitaxy grown GaAs(110) as a material which may be used as a derivative standard for calibrating systems possessing a solid state polarized electron source. The near-bandgap spin polarization of photoelectrons emitted from this material has been characterized for a variety of conditions and several laboratories which possess well calibrated polarimeters have measured the photoelectron polarization of cathodes cut from a common wafer. Despite instrumentation differences, the spread in the measurements is sufficiently small that this material may be used as a derivative calibration standard.

  8. Uncertainty estimates for derivatives and intercepts

    SciTech Connect (OSTI)

    Clark, E.L.

    1990-01-01

    Straight line least squares fits of experimental data are widely used in the analysis of test results to provide derivatives and intercepts. A method for evaluating the uncertainty in these parameters is described. The method utilizes conventional least squares results and is applicable to experiments where the independent variable is controlled, but not necessarily free of error. A Monte Carlo verification of the method is given 7 refs., 2 tabs.

  9. Uncertainty estimates for derivatives and intercepts

    SciTech Connect (OSTI)

    Clark, E.L.

    1994-09-01

    Straight line least squares fits of experimental data are widely used in the analysis of test results to provide derivatives and intercepts. A method for evaluating the uncertainty in these parameters is described. The method utilizes conventional least squares results and is applicable to experiments where the independent variable is controlled, but not necessarily free of error. A Monte Carlo verification of the method is given.

  10. Triamine chelants, their derivatives, complexes and conjugates

    DOE Patents [OSTI]

    Troutner, D.E.; John, C.S.; Pillai, M.R.A.

    1995-03-07

    A group of functionalized triamine chelants and their derivatives that form complexes with radioactive metal ions are disclosed. The complexes can be covalently attached to a protein or an antibody or antibody fragment and used for therapeutic and/or diagnostic purposes. The chelants are of the formula, as shown in the accompanying diagrams, wherein n, m, R, R{sup 1}, R{sup 2} and L are defined in the specification.

  11. Enhanced Coset Symmetries and Higher Derivative Corrections

    E-Print Network [OSTI]

    Neil Lambert; Peter West

    2006-08-17

    After dimensional reduction to three dimensions, the lowest order effective actions for pure gravity, M-theory and the Bosonic string admit an enhanced symmetry group. In this paper we initiate study of how this enhancement is affected by the inclusion of higher derivative terms. In particular we show that the coefficients of the scalar fields associated to the Cartan subalgebra are given by weights of the enhanced symmetry group.

  12. Two-point derivative dispersion relations

    E-Print Network [OSTI]

    Erasmo Ferreira; Javier Sesma

    2014-03-24

    A new derivation is given for the representation, under certain conditions, of the integral dispersion relations of scattering theory through local forms. The resulting expressions have been obtained through an independent procedure to construct the real part, and consist of new mathematical structures of double infinite summations of derivatives. In this new form the derivatives are calculated at the generic value of the energy $E$ and separately at the reference point $E=m$ that is the lower limit of the integration. This new form may be more interesting in certain circumstances and directly shows the origin of the difficulties in convergence that were present in the old truncated forms called standard-DDR. For all cases in which the reductions of the double to single sums were obtained in our previous work, leading to explicit demonstration of convergence, these new expressions are seen to be identical to the previous ones. We present, as a glossary, the most simplified explicit results for the DDR's in the cases of imaginary amplitudes of forms $(E/m)^\\lambda[\\ln (E/m)]^n$, that cover the cases of practical interest in particle physics phenomenology at high energies. We explicitly study the expressions for the cases with $\\lambda$ negative odd integers, that require identification of cancelation of singularities, and provide the corresponding final results.

  13. Acid Fracture and Fracture Conductivity Study of Field Rock Samples 

    E-Print Network [OSTI]

    Underwood, Jarrod

    2013-11-15

    Acid fracturing is a well stimulation strategy designed to increase the productivity of a producing well. The parameters of acid fracturing and the effects of acid interaction on specific rock samples can be studied experimentally. Acid injection...

  14. Micro-electro-mechanical systems phosphoric acid fuel cell

    DOE Patents [OSTI]

    Sopchak, David A. (Livermore, CA); Morse, Jeffrey D. (Martinez, CA); Upadhye, Ravindra S. (Pleasanton, CA); Kotovsky, Jack (Oakland, CA); Graff, Robert T. (Modesto, CA)

    2010-08-17

    A phosphoric acid fuel cell system comprising a porous electrolyte support, a phosphoric acid electrolyte in the porous electrolyte support, a cathode electrode contacting the phosphoric acid electrolyte, and an anode electrode contacting the phosphoric acid electrolyte.

  15. Micro-electro-mechanical systems phosphoric acid fuel cell

    DOE Patents [OSTI]

    Sopchak, David A. (Livermore, CA); Morse, Jeffrey D. (Martinez, CA); Upadhye, Ravindra S. (Pleasanton, CA); Kotovsky, Jack (Oakland, CA); Graff, Robert T. (Modesto, CA)

    2010-12-21

    A phosphoric acid fuel cell system comprising a porous electrolyte support, a phosphoric acid electrolyte in the porous electrolyte support, a cathode electrode contacting the phosphoric acid electrolyte, and an anode electrode contacting the phosphoric acid electrolyte.

  16. Transformation of spatial and perturbation derivatives of travel time

    E-Print Network [OSTI]

    Cerveny, Vlastislav

    Transformation of spatial and perturbation derivatives of travel time at a general interface and perturbation parameters. We derive the explicit equations for transforming these travel­time derivatives Hamiltonian function and are applicable to the transformation of travel­time derivatives in both isotropic

  17. Contemporary Mathematics Derived Canonical Algebras as One-Point Extensions

    E-Print Network [OSTI]

    Barot, Michael

    Contemporary Mathematics Derived Canonical Algebras as One-Point Extensions Michael Barot-dimensional algebra by a -module M is derived canonical, i.e. derived equivalent to a canonical algebra. We give the conditions are even sufficient. As a further result we obtain that, if is derived canonical then the one

  18. Inorganic-polymer-derived dielectric films

    DOE Patents [OSTI]

    Brinker, C. Jeffrey (Albuquerque, NM); Keefer, Keith D. (Albuquerque, NM); Lenahan, Patrick M. (State College, PA)

    1987-01-01

    A method of coating a substrate with a thin film of a polymer of predetermined porosity comprises depositing the thin film on the substrate from a non-gelled solution comprising at least one hydrolyzable metal alkoxide of a polymeric network forming cation, water, an alcohol compatible with the hydrolysis and the polymerization of the metal alkoxide, and an acid or a base, prior to depositing the film, controlling the structure of the polymer for a given composition of the solution exclusive of the acid or base component and the water component, (a) by adjusting each of the water content, the pH, and the temperature to obtain the desired concentration of alkoxide, and then adjusting the time of standing of the solution prior to lowering the temperature of the solution, and (b) lowering the temperature of the solution after the time of standing to about 15 degrees C. or lower to trap the solution in a state in which, after the depositing step, a coating of the desired porosity will be obtained, and curing the deposited film at a temperature effective for curing whereby there is obtained a thin film of a polymer of a predetermined porosity and corresponding pore size on the substrate.

  19. Inorganic-polymer-derived dielectric films

    DOE Patents [OSTI]

    Brinker, C.J.; Keefer, K.D.; Lenahan, P.M.

    1985-02-25

    A method is disclosed for coating a substrate with a thin film of a predetermined porosity. The method comprises: depositing the thin film on the substrate from a non-gelled solution comprising at least one metal alkoxide of a polymeric network forming cation, water, an alcohol compatible with the hydrolysis and the polymerization of the metal alkoxide, and an acid or a base; prior to said depositing step, controlling the porosity and structure of said coating for a given composition of said solution exclusive of the acid or base component and the water component, by adjusting each of the water content, the pH, the temperature and the time of standing of said solution, increasing/descreasing the water content or the pH to increase/decrease the pore size of said coating, and increasing/decreasing the temperature or time of standing of said solution to increase/decrease the pore size of said coating; and curing said deposited film at a temperature effective for curing whereby there is obtained a thin film coating of a predetermined porosity on the substrate.

  20. D-Amino Acid Utilization in Algae Jennifer Meoni1, Farrah Moazeni2, Gaosen Zhang, Ph.D.2,

    E-Print Network [OSTI]

    Walker, Lawrence R.

    D-Amino Acid Utilization in Algae Jennifer Meoni1, Farrah Moazeni2, Gaosen Zhang, Ph.D.2, Henry Sun are derived primarily from cell wall (peptidoglycan) remnants of bacteria. Algae, which are composed environment so that we are not exposed. However, racemases are not known to be present in algae. So

  1. Double stranded nucleic acid biochips

    DOE Patents [OSTI]

    Chernov, Boris; Golova, Julia

    2006-05-23

    This invention describes a new method of constructing double-stranded DNA (dsDNA) microarrays based on the use of pre-synthesized or natural DNA duplexes without a stem-loop structure. The complementary oligonucleotide chains are bonded together by a novel connector that includes a linker for immobilization on a matrix. A non-enzymatic method for synthesizing double-stranded nucleic acids with this novel connector enables the construction of inexpensive and robust dsDNA/dsRNA microarrays. DNA-DNA and DNA-protein interactions are investigated using the microarrays.

  2. ARM - Lesson Plans: Acid Rain

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Comments?govInstrumentsnoaacrnBarrow, Alaska Outreach Home Room News Publications TraditionalPlans OutreachAcid

  3. The Synthesis of Carboracycles Derived from B,B'-Bis(aryl) Derivatives of Icosahedral ortho-Carborane

    E-Print Network [OSTI]

    Hawthorne, M. Frederick

    The Synthesis of Carboracycles Derived from B,B'-Bis(aryl) Derivatives of Icosahedral ortho)] compounds in high yields. The anisole derivatives 3 and 8 were deprotected to yield the corresponding bis(O)Me). Deprotection of compound 11 led to the corresponding acetyl derivative 18 (R' C(O)Me). Bis- anisole 3

  4. Deriving time from the geometry of space

    E-Print Network [OSTI]

    James M. Chappell; John G. Hartnett; Nicolangelo Iannella; Derek Abbott

    2015-04-08

    The Minkowski formulation of special relativity reveals the essential four-dimensional nature of spacetime, consisting of three space and one time dimension. Recognizing its fundamental importance, a variety of arguments have been proposed over the years attempting to derive the Minkowski spacetime structure from fundamental physical principles. In this paper we illustrate how Minkowski spacetime follows naturally from the geometric properties of three dimensional Clifford space modeled with multivectors. This approach also generalizes spacetime to an eight dimensional space as well as doubling the size of the Lorentz group. This description of spacetime also provides a new geometrical interpretation of the nature of time.

  5. Derivation of an Applied Nonlinear Schroedinger Equation.

    SciTech Connect (OSTI)

    Pitts, Todd Alan; Laine, Mark Richard; Schwarz, Jens; Rambo, Patrick K.; Karelitz, David B.

    2015-01-01

    We derive from first principles a mathematical physics model useful for understanding nonlinear optical propagation (including filamentation). All assumptions necessary for the development are clearly explained. We include the Kerr effect, Raman scattering, and ionization (as well as linear and nonlinear shock, diffraction and dispersion). We explain the phenomenological sub-models and each assumption required to arrive at a complete and consistent theoretical description. The development includes the relationship between shock and ionization and demonstrates why inclusion of Drude model impedance effects alters the nature of the shock operator. Unclassified Unlimited Release

  6. Complete Genome Sequence of a thermotolerant sporogenic lactic acid bacterium, Bacillus coagulans strain 36D1

    SciTech Connect (OSTI)

    Rhee, Mun Su [University of Florida, Gainesville; Moritz, Brelan E. [University of Florida, Gainesville; Xie, Gary [Los Alamos National Laboratory (LANL); Glavina Del Rio, Tijana [U.S. Department of Energy, Joint Genome Institute; Dalin, Eileen [U.S. Department of Energy, Joint Genome Institute; Tice, Hope [U.S. Department of Energy, Joint Genome Institute; Bruce, David [Los Alamos National Laboratory (LANL); Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Chertkov, Olga [Los Alamos National Laboratory (LANL); Brettin, Thomas S [ORNL; Han, Cliff [Los Alamos National Laboratory (LANL); Detter, J. Chris [U.S. Department of Energy, Joint Genome Institute; Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Land, Miriam L [ORNL; Patel, Milind [University of Florida, Gainesville; Ou, Mark [University of Florida, Gainesville; Harbrucker, Roberta [University of Florida, Gainesville; Ingram, Lonnie O. [University of Florida; Shanmugam, Keelnathan T. [University of Florida

    2011-01-01

    Bacillus coagulans is a ubiquitous soil bacterium that grows at 50-55 C and pH 5.0 and fer- ments various sugars that constitute plant biomass to L (+)-lactic acid. The ability of this spo- rogenic lactic acid bacterium to grow at 50-55 C and pH 5.0 makes this organism an attrac- tive microbial biocatalyst for production of optically pure lactic acid at industrial scale not only from glucose derived from cellulose but also from xylose, a major constituent of hemi- cellulose. This bacterium is also considered as a potential probiotic. Complete genome se- quence of a representative strain, B. coagulans strain 36D1, is presented and discussed.

  7. Self-assembling multimeric nucleic acid constructs

    DOE Patents [OSTI]

    Cantor, Charles R. (Boston, MA); Niemeyer, Christof M. (Bremen, DE); Smith, Cassandra L. (Boston, MA); Sano, Takeshi (Boston, MA); Hnatowich, Donald J. (Brookline, MA); Rusckowski, Mary (Southborough, MA)

    1996-01-01

    The invention is directed to constructs and compositions containing multimeric forms of nucleic acid. Multimeric nucleic acids comprise single-stranded nucleic acids attached via biotin to streptavidin and bound with a functional group. These constructs can be utilized in vivo to treat or identify diseased tissue or cells. Repeated administrations of multimeric nucleic acid compositions produce a rapid and specific amplification of nucleic acid constructs and their attached functional groups. For treatment purposes, functional groups may be toxins, radioisotopes, genes or enzymes. Diagnostically, labeled multimeric constructs may be used to identify specific targets in vivo or in vitro. Multimeric nucleic acids may also be used in nanotechnology and to create self-assembling polymeric aggregates such as membranes of defined porosity, microcircuits and many other products.

  8. Self-assembling multimeric nucleic acid constructs

    DOE Patents [OSTI]

    Cantor, Charles R. (Boston, MA); Niemeyer, Christof M. (Bremen, DE); Smith, Cassandra L. (Boston, MA); Sano, Takeshi (Boston, MA); Hnatowich, Donald J. (Brookline, MA); Rusckowski, Mary (Southborough, MA)

    1999-10-12

    The invention is directed to constructs and compositions containing multimeric forms of nucleic acid. Multimeric nucleic acids comprise single-stranded nucleic acids attached via biotin to streptavidin and bound with a functional group. These constructs can be utilized in vivo to treat or identify diseased tissue or cells. Repeated administrations of multimeric nucleic acid compositions produce a rapid and specific amplification of nucleic acid constructs and their attached functional groups. For treatment purposes, functional groups may be toxins, radioisotopes, genes or enzymes. Diagnostically, labeled multimeric constructs may be used to identify specific targets in vivo or in vitro. Multimeric nucleic acids may also be used in nanotechnology and to create self-assembling polymeric aggregates such as membranes of defined porosity, microcircuits and many other products.

  9. Self-assembling multimeric nucleic acid constructs

    DOE Patents [OSTI]

    Cantor, C.R.; Niemeyer, C.M.; Smith, C.L.; Sano, Takeshi; Hnatowich, D.J.; Rusckowski, M.

    1996-10-01

    The invention is directed to constructs and compositions containing multimeric forms of nucleic acid. Multimeric nucleic acids comprise single-stranded nucleic acids attached via biotin to streptavidin and bound with a functional group. These constructs can be utilized in vivo to treat or identify diseased tissue or cells. Repeated administrations of multimeric nucleic acid compositions produce a rapid and specific amplification of nucleic acid constructs and their attached functional groups. For treatment purposes, functional groups may be toxins, radioisotopes, genes or enzymes. Diagnostically, labeled multimeric constructs may be used to identify specific targets in vivo or in vitro. Multimeric nucleic acids may also be used in nanotechnology and to create self-assembling polymeric aggregates such as membranes of defined porosity, microcircuits and many other products. 5 figs.

  10. Protein location prediction using atomic composition and global features of the amino acid sequence

    SciTech Connect (OSTI)

    Cherian, Betsy Sheena, E-mail: betsy.skb@gmail.com [Centre for Bioinformatics, University of Kerala, Kariyavattom Campus, Thiruvananthapuram, Kerala (India); Nair, Achuthsankar S. [Centre for Bioinformatics, University of Kerala, Kariyavattom Campus, Thiruvananthapuram, Kerala (India)] [Centre for Bioinformatics, University of Kerala, Kariyavattom Campus, Thiruvananthapuram, Kerala (India)

    2010-01-22

    Subcellular location of protein is constructive information in determining its function, screening for drug candidates, vaccine design, annotation of gene products and in selecting relevant proteins for further studies. Computational prediction of subcellular localization deals with predicting the location of a protein from its amino acid sequence. For a computational localization prediction method to be more accurate, it should exploit all possible relevant biological features that contribute to the subcellular localization. In this work, we extracted the biological features from the full length protein sequence to incorporate more biological information. A new biological feature, distribution of atomic composition is effectively used with, multiple physiochemical properties, amino acid composition, three part amino acid composition, and sequence similarity for predicting the subcellular location of the protein. Support Vector Machines are designed for four modules and prediction is made by a weighted voting system. Our system makes prediction with an accuracy of 100, 82.47, 88.81 for self-consistency test, jackknife test and independent data test respectively. Our results provide evidence that the prediction based on the biological features derived from the full length amino acid sequence gives better accuracy than those derived from N-terminal alone. Considering the features as a distribution within the entire sequence will bring out underlying property distribution to a greater detail to enhance the prediction accuracy.

  11. Primer on electricity futures and other derivatives

    SciTech Connect (OSTI)

    Stoft, S.; Belden, T.; Goldman, C.; Pickle, S.

    1998-01-01

    Increased competition in bulk power and retail electricity markets is likely to lower electricity prices, but will also result in greater price volatility as the industry moves away from administratively determined, cost-based rates and encourages market-driven prices. Price volatility introduces new risks for generators, consumers, and marketers. Electricity futures and other derivatives can help each of these market participants manage, or hedge, price risks in a competitive electricity market. Futures contracts are legally binding and negotiable contracts that call for the future delivery of a commodity. In most cases, physical delivery does not take place, and the futures contract is closed by buying or selling a futures contract on or near the delivery date. Other electric rate derivatives include options, price swaps, basis swaps, and forward contracts. This report is intended as a primer for public utility commissioners and their staff on futures and other financial instruments used to manage price risks. The report also explores some of the difficult choices facing regulators as they attempt to develop policies in this area.

  12. Acid rain information book. Draft final report

    SciTech Connect (OSTI)

    None

    1980-12-01

    Acid rain is one of the most widely publicized environmental issues of the day. The potential consequences of increasingly widespread acid rain demand that this phenomenon be carefully evaluated. Reveiw of the literature shows a rapidly growing body of knowledge, but also reveals major gaps in understanding that need to be narrowed. This document discusses major aspects of the acid rain phenomenon, points out areas of uncertainty, and summarizes current and projected research by responsible government agencies and other concerned organizations.

  13. Carboxylic acid accelerated formation of diesters

    DOE Patents [OSTI]

    Tustin, G.C.; Dickson, T.J.

    1998-04-28

    This invention pertains to accelerating the rate of formation of 1,1-dicarboxylic esters from the reaction of an aldehyde with a carboxylic acid anhydride or a ketene in the presence of a non-iodide containing a strong Bronsted acid catalyst by the addition of a carboxylic acid at about one bar pressure and between about 0 and 80 C in the substantial absence of a hydrogenation or carbonylation catalyst.

  14. Understanding Naphthenic Acid Corrosion in Refinery Settings

    E-Print Network [OSTI]

    Patrick, Brian Neil

    2015-01-01

    R. & Tordo, S. , 2005. Crude oil price differentials andfiles/08105.Technical Paper_Crude Oil Price DifferentialsAcids Present in Crude Oils Using Nanospray Fourier

  15. Investigating intermediates in 6-methylsalicylic acid biosynthesis

    E-Print Network [OSTI]

    Potter, Helen Katherine

    2011-07-12

    .3.1 Chlorination ............................................................................................ 174 A2.3.2 Coupling of amino acids.......................................................................... 174 A2.3.3 Protecting groups...

  16. Acid Doped Membranes for High Temperature PEMFC

    Broader source: Energy.gov [DOE]

    Presentation on Acid Doped Membranes for High Temperature PEMFC to the High Temperature Membrane Working Group, May 25, 2004 in Philadelphia, PA.

  17. Bioconversion of coal-derived synthesis gas to liquid fuels. [Butyribacterium methylotrophicum

    SciTech Connect (OSTI)

    Jain, M.K.

    1991-01-01

    The use of coal-derived synthesis gas as an industrial feedstock for production of fuels and chemicals has become an increasingly attractive alternative to present petroleum-based chemicals production. However, one of the major limitations in developing such a process is the required removal of catalyst poisons such as hydrogen sulfide (H{sub 2}S), carbonyl sulfide (COS), and other trace contaminants from the synthesis gas. Purification steps necessary to remove these are energy intensive and add significantly to the production cost, particularly for coals having a high sulfur content such as Illinois coal. A two-stage, anaerobic bioconversion process requiring little or no sulfur removal is proposed, where in the first stage the carbon monoxide (CO) gas is converted to butyric and acetic acids by the CO strain of Butyribacterium methylotrophicum. In the second stage, these acids along with the hydrogen (H{sub 2}) gas are converted to butanol, ethanol, and acetone by an acid utilizing mutant of Clostridium acetobutylicum. 18 figs., 18 tabs.

  18. Organic acid-tolerant microorganisms and uses thereof for producing organic acids

    DOE Patents [OSTI]

    Pfleger, Brian Frederick; Begemann, Matthew Brett

    2014-05-06

    Organic acid-tolerant microorganisms and methods of using same. The organic acid-tolerant microorganisms comprise modifications that reduce or ablate AcsA activity or AcsA homolog activity. The modifications increase tolerance of the microorganisms to such organic acids as 3-hydroxypropionic acid (3HP), acrylic acid, and propionic acid. Further modifications to the microorganisms such as increasing expression of malonyl-CoA reductase and/or acetyl-CoA carboxylase provide or increase the ability of the microorganisms to produce 3HP. Methods of generating an organic acid with the modified microorganisms are provided. Methods of using acsA or homologs thereof as counter-selectable markers include replacing acsA or homologs thereof in cells with genes of interest and selecting for the cells comprising the genes of interest with amounts of organic acids effective to inhibit growth of cells harboring acsA or the homologs.

  19. Effects of Acid Additives on Spent Acid Flowback through Carbonate Cores 

    E-Print Network [OSTI]

    Nasir, Ehsaan Ahmad

    2012-07-16

    these challenges, different chemicals, or additives, are added to the acid solution such as corrosion inhibitors and iron control agents. These additives may change the relative permeability of the spent acid, and formation wettability, and may either hinder...

  20. A method to attenuate U(VI) mobility in acidic waste plumes using humic acids

    E-Print Network [OSTI]

    Wan, J.

    2011-01-01

    base properties of a goethite surface model: A theoreticalcomplexation of U(VI) on goethite (alpha-FeOOH). Geochim.acid and humic-acid on goethite, gibbsite and imogolite. J.

  1. Experimental Investigation on the Effect of Permeability on the Optimum Acid Flux in Carbonate Matrix Acidizing 

    E-Print Network [OSTI]

    Etten, Jordan Ruby

    2015-05-05

    stimulation design. Optimum interstitial velocity determines the injection rate for a treatment, and the optimum pore volume to breakthrough, PVbt-opt, suggests the total volume of acid needed. Studies of carbonate matrix acidizing have focused on the role...

  2. Non-essential amino acid metabolism in rats 

    E-Print Network [OSTI]

    Crooks, James Darrell

    1971-01-01

    -bound amino acids were studied' The amino acids studied were asparagine, aspartic acid, glutamine, glutamic acid, glycine, alanine, arginine, and praline. Growth rates of rats increased with increased levels of glutamate in the diet. Effects of level... of dietary glutamic acid on endogenous pool size;, of the free amino acids were studied. No consistent patterns were observed. The pool sizes of all but four of the free amino acids increased after the ingestion of a meal. Glucose levels decreased...

  3. Automation of Nested Matrix and Derivative Operations Robert Kalaba

    E-Print Network [OSTI]

    Tesfatsion, Leigh

    Automation of Nested Matrix and Derivative Operations Robert Kalaba Departments of Electrical of special functions a=x, b=y, c = ab, (4) a=log(c), z=a+d. #12;Automation of Matrix Derivative Operations

  4. Higher Derivative Corrections to O-Plane Actions 

    E-Print Network [OSTI]

    Wang, Zhao

    2014-11-17

    Higher derivative corrections to effective actions are very important and of great interest in string theory. The aim of this dissertation is to develop a method to constrain the higher derivative corrections to O-plane ...

  5. Optimal design of derivatives in illiquid market: an alternative approach

    E-Print Network [OSTI]

    Vargiolu, Tiziano

    by Barrieu and El Karoui in [1, 2] for the design of derivatives in illiquid markets. Its motivation is the Optimal design of derivatives in illiquid * market: an alternative approach Grazia De Silvestro

  6. Symbolic Neural Networks Derived from Stochastic Grammar Domain Models 1 Symbolic Neural Networks Derived from Stochastic

    E-Print Network [OSTI]

    Mjolsness, Eric

    Symbolic Neural Networks Derived from Stochastic Grammar Domain Models 1 Symbolic Neural Networks neural network architectures with some of the expressive power of a semantic network and also some of the pattern recognition and learning capabilities of more conventional neural networks. For example

  7. Producing dicarboxylic acids using polyketide synthases

    DOE Patents [OSTI]

    Katz, Leonard; Fortman, Jeffrey L; Keasling, Jay D

    2013-10-29

    The present invention provides for a polyketide synthase (PKS) capable of synthesizing a dicarboxylic acid (diacid). Such diacids include diketide-diacids and triketide-diacids. The invention includes recombinant nucleic acid encoding the PKS, and host cells comprising the PKS. The invention also includes methods for producing the diacids.

  8. Nitrates and Prussic Acid in Forages 

    E-Print Network [OSTI]

    Provin, Tony; Pitt, John L.

    2003-01-06

    When nitrates and prussic acid accumulate in forage, the feed may not be safe for livestock consumption. Learn the symptoms of nitrate and prussic acid poisoning and which plants are most likely to pose a risk to livestock. Also learn sampling...

  9. Nanoparticles modified with multiple organic acids

    DOE Patents [OSTI]

    Cook, Ronald Lee (Lakewood, CO); Luebben, Silvia DeVito (Golden, CO); Myers, Andrew William (Arvada, CO); Smith, Bryan Matthew (Boulder, CO); Elliott, Brian John (Superior, CO); Kreutzer, Cory (Brighton, CO); Wilson, Carolina (Arvada, CO); Meiser, Manfred (Aurora, CO)

    2007-07-17

    Surface-modified nanoparticles of boehmite, and methods for preparing the same. Aluminum oxyhydroxide nanoparticles are surface modified by reaction with selected amounts of organic acids. In particular, the nanoparticle surface is modified by reactions with two or more different carboxylic acids, at least one of which is an organic carboxylic acid. The product is a surface modified boehmite nanoparticle that has an inorganic aluminum oxyhydroxide core, or part aluminum oxyhydroxide core and a surface-bonded organic shell. Organic carboxylic acids of this invention contain at least one carboxylic acid group and one carbon-hydrogen bond. One embodiment of this invention provides boehmite nanoparticles that have been surface modified with two or more acids one of which additional carries at least one reactive functional group. Another embodiment of this invention provides boehmite nanoparticles that have been surface modified with multiple acids one of which has molecular weight or average molecular weight greater than or equal to 500 Daltons. Yet, another embodiment of this invention provides boehmite nanoparticles that are surface modified with two or more acids one of which is hydrophobic in nature and has solubility in water of less than 15 by weight. The products of the methods of this invention have specific useful properties when used in mixture with liquids, as filler in solids, or as stand-alone entities.

  10. Amino acid analogs for tumor imaging

    DOE Patents [OSTI]

    Goodman, M.M.; Shoup, T.

    1998-10-06

    The invention provides novel amino acid compounds of use in detecting and evaluating brain and body tumors. These compounds combine the advantageous properties of 1-amino-cycloalkyl-1-carboxylic acids, namely, their rapid uptake and prolonged retention in tumors with the properties of halogen substituents, including certain useful halogen isotopes including fluorine-18, iodine-123, iodine-125, iodine-131, bromine-75, bromine-76, bromine-77 and bromine-82. In one aspect, the invention features amino acid compounds that have a high specificity for target sites when administered to a subject in vivo. Preferred amino acid compounds show a target to non-target ratio of at least 5:1, are stable in vivo and substantially localized to target within 1 hour after administration. An especially preferred amino acid compound is [{sup 18}F]-1-amino-3-fluorocyclobutane-1-carboxylic acid (FACBC). In another aspect, the invention features pharmaceutical compositions comprised of an {alpha}-amino acid moiety attached to either a four, five, or a six member carbon-chain ring. In addition, the invention features analogs of {alpha}-aminoisobutyric acid.

  11. Amino acid analogs for tumor imaging

    DOE Patents [OSTI]

    Goodman, M.M.; Shoup, T.

    1998-09-15

    The invention provides novel amino acid compounds of use in detecting and evaluating brain and body tumors. These compounds combine the advantageous properties of 1-amino-cycloalkyl-1-carboxylic acids, namely, their rapid uptake and prolonged retention in tumors with the properties of halogen substituents, including certain useful halogen isotopes including fluorine-18, iodine-123, iodine-125, iodine-131, bromine-75, bromine-76, bromine-77 and bromine-82. In one aspect, the invention features amino acid compounds that have a high specificity for target sites when administered to a subject in vivo. Preferred amino acid compounds show a target to non-target ratio of at least 5:1, are stable in vivo and substantially localized to target within 1 hour after administration. An especially preferred amino acid compound is [{sup 18}F]-1-amino-3-fluorocyclobutane-1-carboxylic acid (FACBC). In another aspect, the invention features pharmaceutical compositions comprised of an {alpha}-amino acid moiety attached to either a four, five, or a six member carbon-chain ring. In addition, the invention features analogs of {alpha}-aminoisobutyric acid.

  12. Amino acid analogs for tumor imaging

    DOE Patents [OSTI]

    Goodman, Mark M. (Atlanta, GA); Shoup, Timothy (Decatur, GA)

    1998-09-15

    The invention provides novel amino acid compounds of use in detecting and evaluating brain and body tumors. These compounds combine the advantageous properties of 1-amino-cycloalkyl-1-carboxylic acids, namely, their rapid uptake and prolonged retention in tumors with the properties of halogen substituents, including certain useful halogen isotopes including fluorine-18, iodine-123, iodine-125, iodine-131, bromine-75, bromine-76, bromine-77 and bromine-82. In one aspect, the invention features amino acid compounds that have a high specificity for target sites when administered to a subject in vivo. Preferred amino acid compounds show a target to non-target ratio of at least 5:1, are stable in vivo and substantially localized to target within 1 hour after administration. An especially preferred amino acid compound is ›.sup.18 F!-1-amino-3-fluorocyclobutane-1-carboxylic acid (FACBC). In another aspect, the invention features pharmaceutical compositions comprised of an .alpha.-amino acid moiety attached to either a four, five, or a six member carbon-chain ring. In addition, the invention features analogs of .alpha.-aminoisobutyric acid.

  13. Amino acid analogs for tumor imaging

    DOE Patents [OSTI]

    Goodman, Mark M. (Atlanta, GA); Shoup, Timothy (Decatur, GA)

    1998-10-06

    The invention provides novel amino acid compounds of use in detecting and evaluating brain and body tumors. These compounds combine the advantageous properties of 1-amino-cycloalkyl-1-carboxylic acids, namely, their rapid uptake and prolonged retention in tumors with the properties of halogen substituents, including certain useful halogen isotopes including fluorine-18, iodine-123, iodine-125, iodine-131, bromine-75, bromine-76, bromine-77 and bromine-82. In one aspect, the invention features amino acid compounds that have a high specificity for target sites when administered to a subject in vivo. Preferred amino acid compounds show a target to non-target ratio of at least 5:1, are stable in vivo and substantially localized to target within 1 hour after administration. An especially preferred amino acid compound is ›.sup.18 F!-1-amino-3-fluorocyclobutane-1-carboxylic acid (FACBC). In another aspect, the invention features pharmaceutical compositions comprised of an .alpha.-amino acid moiety attached to either a four, five, or a six member carbon-chain ring. In addition, the invention features analogs of .alpha.-aminoisobutyric acid.

  14. Organic Acid Production by Filamentous Fungi

    E-Print Network [OSTI]

    12 Organic Acid Production by Filamentous Fungi Jon K. Magnuson and Linda L. Lasure 1. Introduction Many of the commercial production processes for organic acids are excellent examples of fungal overshadowed by the successful deploy- ment of the -lactam processes.Yet, in terms of productivity, fungal

  15. Petrochemicals from coal-derived syngas

    SciTech Connect (OSTI)

    Sardesai, A.; Lee, S.

    1996-12-31

    The development of the Liquid Phase Dimethyl Ether (LPDME) process has established a means to effectively convert CO-rich syngas to dimethyl ether (DME) in a mechanically agitated slurry reactor. By operating in a dual catalyst mode, in-situ produced methanol may be converted to DME, thereby alleviating the chemical equilibrium limitation imposed on the methanol synthesis reaction. As a result, higher syngas conversions and methyl productivities are seen over methanol synthesis alone. This effective route to DME production over methanol has led to the development of conversion technologies based on a DME feedstock. Oxygenates, in particular, ethers and their precursors, are very important as potential clean fuel additives and have been postulated through vinylation/hydrogenation and oxidative coupling reactions. Specialty chemicals such as methyl acetate and acetic acid have widescale industrial importance in the conversion to ethanol from a non-agricultural feedstock. Vapor phase oxidative dimerization of DME over tin based catalysts produced precursors of ethylene glycol. Finally, DME has been extensively used as a feedstock for hydrocarbon synthesis including olefins, paraffins and gasoline range hydrocarbons, over zeolite based catalysts with a 46% increase in product selectivity over methanol. The efficient production of DME in the liquid phase has given it widescale industrial significance as a potential replacement for methanol and as a keystone for more important petrochemicals.

  16. Derivative Securities G63.2791.001, Fall 2007

    E-Print Network [OSTI]

    -based pricing of derivative securities. Topics include: arbitrage; risk-neutral valuation; the log, and other interest-based derivatives; credit risk and credit derivatives. This section versus Steve Allen, one every couple of weeks. Col- laboration on homework is encouraged (homeworks are not exams

  17. Derivations of Marcus's formula G.F. Bertsch1

    E-Print Network [OSTI]

    Bertsch George F.

    Derivations of Marcus's formula G.F. Bertsch1 1 Institute for Nuclear Theory and Dept. of Physics, University of Washington, Seattle, Washington Abstract Two derivations of Marcus's formula for transition rates are presented. The first derivation is based on the Landau-Zener transition rate formula

  18. Biomass-Derived Energy Products and Co-Products Market

    E-Print Network [OSTI]

    Biomass-Derived Energy Products and Co-Products Market This report identifies the bio-fuels and co & Earth Science & Technology ­ University of Hawai`i at Manoa #12;Biomass-Derived Energy Products and Co agency thereof. #12;Biomass Derived Energy Products and Co- Products Market and Off-take Study Hawaii

  19. Carbohydrate-derived aminoalcohol ligands for asymmetric Reformatsky reactions

    E-Print Network [OSTI]

    Davis, Ben G.

    Carbohydrate-derived aminoalcohol ligands for asymmetric Reformatsky reactions Daniel P. G of functionally and stereochemically diverse DD-glucosamine-derived tertiary aminoalcohol ligands have been used bromoacetate-3 and difluorobromoacetate4 -derived Reformatsky reagents 2a­c (Scheme 1). A general, enan

  20. RNA Interference: Endogenous siRNAs Derived from Transposable

    E-Print Network [OSTI]

    Jiggins, Francis

    RNA Interference: Endogenous siRNAs Derived from Transposable Elements The Piwi-interacting RNARNAs are about 22 nt long, are derived from host-expressed fold-back structures, and associate primarily with Ago1 [5]. Conversely, antiviral siRNAs (viRNAs), are about 21 nt, are derived from double