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1

Genetics of x-ray induced double strand break repair in saccharomyces cerevisiae  

SciTech Connect

The possible fates of x-ray-induced double-strand breaks in Saccharomyces cerevisiae were examined. One possible pathway which breaks can follow, the repair pathway, was studied by assaying strains with mutations in the RAD51, RAD54, and RAD57 loci for double-strand break repair. In order of increasing radiation sensitivity one finds: rad57-1(23/sup 0/)> rad51-1(30/sup 0/)> rad54-3(36/sup 0/). At 36/sup 0/, rad54-3 cells cannot repair double-strand breaks, while 23/sup 0/, they can. Strains with the rad57-1 mutation can rejoin broken chromosomes at both temperatures. However, the low survival at 36/sup 0/ shows that the assay is not distinguishing large DNA fragments which allow cell survival from those which cause cell death. A rad51-1 strain could also rejoin broken chromosomes, and was thus capable of incomplete repair. The data can be explained with the hypothesis that rad54-3 cells are blocked in an early step of repair, while rad51-1 and rad57-1 strains are blocked in a later step of repair. The fate of double-strand breaks when they are left unrepaired was investigated with the rad54-3 mutation. If breaks are prevented from entering the RAD54 repair pathway they become uncommitted lesions. These lesions are repaired slower than the original breaks. One possible fate for an uncommitted lesion is conversion into a fixed lesion, which is likely to be an unrepairable or misrepaired double-strand break. The presence of protein synthesis after irradiation increases the probability that a break will enter the repair pathway. Evidence shows that increased probability of repair results from enhanced synthesis of repair proteins shortly after radiation. (ERB)

Budd, M.E.

1982-07-01T23:59:59.000Z

2

The Role of DNA double-strand break repair in cellular response to low  

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DNA double-strand break repair in cellular response to low DNA double-strand break repair in cellular response to low dose radiation exposure. David J. Chen Division of Molecular Radiation Biology, Department of Radiation Oncology, University of Texas Southwestern Medical Center, Dallas TX 75390 It has been assumed that molecular pathways that involved in the biological response for low dose of radiation should be similar to those for high dose radiation in general. The low dose-rate effect and DNA double-strand break repair are inextricably linked in mammalian cells. It has been reported that mammalian mutant cells deficient in nonhomologous end join (NHEJ) pathway have little or no cellular recovery when expose to low-dose-rate radiation. Recently, it has also been reported that cells deficient in

3

ASSESSMENT OF INDIVIDUAL VARIATION IN DNA DOUBLE-STRAND BREAK REPAIR CAPACITY IN HUMAN DIPLOID FIBROBLASTS  

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ASSESSMENT OF INDIVIDUAL VARIATION IN DNA DOUBLE-STRAND ASSESSMENT OF INDIVIDUAL VARIATION IN DNA DOUBLE-STRAND BREAK REPAIR CAPACITY IN HUMAN DIPLOID FIBROBLASTS Paul F. Wilson, John M. Hinz, Peter B. Nham, Salustra S. Urbin, Cynthia B. Thomas, Irene M. Jones, and Larry H. Thompson Biosciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA The induction of bi-stranded clustered DNA damage (BCD), which includes direct DNA double-strand breaks (DSBs), is a hallmark of ionizing radiation (IR) exposure. Incorrectly repaired DSBs can cause chromosomal rearrangements and an increased risk of genomic instability and cancer. Because there is polymorphic variation in DNA repair genes and much of this variation is predicted to have a functional impact, healthy people likely vary in their capacity to repair DSBs and other BCD. This project

4

A Key Step in Repairing DNA Double-Strand Breaks | Advanced Photon Source  

NLE Websites -- All DOE Office Websites (Extended Search)

X-ray Rainbow X-ray Rainbow An Insulating Breakthrough Science Highlights Archives: 2013 | 2012 | 2011 | 2010 2009 | 2008 | 2007 | 2006 2005 | 2004 | 2003 | 2002 2001 | 2000 | 1998 | Subscribe to APS Science Highlights rss feed A Key Step in Repairing DNA Double-Strand Breaks JANUARY 10, 2007 Bookmark and Share The atomic structure of the protein 53BP1 identified by Mayo researchers. (Courtesy: Mayo Clinic) A team of Mayo Clinic researchers has uncovered a key step in the molecular pathway of repairing DNA double-strand breaks. The studies were carried out using the 19-ID beamline (SBC-CAT) at the Advanced Photon Source (APS, Argonne) and the X12-C beamline at the National Synchrotron Light Source (NSLS, Brookhaven National Laboratory). Both the APS and the NSLS are funded by the U.S. Department of Energy's Office of Basic Energy

5

Repair of Site-Specific DNA Double-Strand Breaks in Barley Occurs via Diverse Pathways Primarily Involving the Sister Chromatid  

Science Journals Connector (OSTI)

...research-article RESEARCH ARTICLES Repair of Site-Specific DNA Double-Strand...various double-strand break repair pathways in the monocot plant...0.307_win32 with UPARSE pipeline (Edgar, 2013). The three...polymorphisms indicative of distinct repair mechanisms. SCE and Mitotic...

Giang T.H. Vu; Hieu X. Cao; Koichi Watanabe; Goetz Hensel; Frank R. Blattner; Jochen Kumlehn; Ingo Schubert

2014-05-29T23:59:59.000Z

6

Epigenetic Modifications in Double-Strand Break DNA Damage Signaling and Repair  

Science Journals Connector (OSTI)

...Focus 10 14 31 DNA Damage and Repair in Translational Oncology...investigators, DNA damage and repair has been a major focus of anticancer...Plummer R . Perspective on the pipeline of drugs being developed with...break DNA damage signaling and repair. Clin Cancer Res 2010;16...

Dorine Rossetto; Andrew W. Truman; Stephen J. Kron; and Jacques Côté

2010-09-15T23:59:59.000Z

7

The BLM Helicase Is Necessary for Normal DNA Double-Strand Break Repair  

Science Journals Connector (OSTI)

...control, corrected BS, BS, and AT cells in a DSB...EcoRI-cut CIP pUC18 in nuclear extracts are shown. Part...DSB repair assays. The costs of publication of this...abbreviations used are: BS, Blooms syndrome; AT...of linearized supF20 by nuclear extracts from control...

Gregory Langland; James Elliott; Yuling Li; Jenette Creaney; Kathleen Dixon; and Joanna Groden

2002-05-15T23:59:59.000Z

8

Human Polynucleotide Kinase Participates in Repair of DNA Double-Strand Breaks by Nonhomologous End Joining but not Homologous Recombination  

Science Journals Connector (OSTI)

...for the repair of topoisomerase I-mediated DNA lesions. DNA Repair (Amst) 2003;2:1087-100. 42 El-Khamisy SF, Saifi GM, Weinfeld M, et al. Defective DNA single-strand break repair in spinocerebellar ataxia with axonal neuropathy-1...

Feridoun Karimi-Busheri; Aghdass Rasouli-Nia; Joan Allalunis-Turner; Michael Weinfeld

2007-07-15T23:59:59.000Z

9

Natural insertions in rice commonly form tandem duplications indicative of patch-mediated double-strand break induction and repair  

Science Journals Connector (OSTI)

...high-throughput sequencing of DSB repair events in humans...conventional models of DSB repair are strained to predict...proposing that long patch base excision repair (BER) on complementary...in their short-read pipeline were true positives...

Justin N. Vaughn; Jeffrey L. Bennetzen

2014-01-01T23:59:59.000Z

10

Gene Expression Pathways Activated and Repair of DNA of Double Strand  

NLE Websites -- All DOE Office Websites (Extended Search)

Pathways Activated and Repair of DNA of Double Strand Pathways Activated and Repair of DNA of Double Strand Breaks (DSBs) Following Low Dose Ionizing Radiation in Human Bronchial Epithelial Cells (HBECs) With and Without Defined Premalignant Oncogenic Changes John Minna University of Texas Southwestern Medical Center at Dallas Abstract We want to determine the effect of low dose ionizing radiation on the molecular progression of lung epithelial cells towards lung cancer. Our endpoints are focused on specific molecular and cellular changes known to be associated with lung cancer development and quantitating those changes as a function of low dose ionizing radiation. We use immortalized human bronchial epithelial cells (HBECs) developed from >40 individuals (including those from current, former, and never smokers, males, females,

11

Ionizing Radiation Damage to the Folded Chromosome of Escherichia coli K-12: Repair of Double-Strand Breaks in Deoxyribonucleic Acid  

Science Journals Connector (OSTI)

...cell survival Dose (krad) % Recovery...it is in the range of sedimentation...that direct, radiation-induced cross-linking...during repair of ionizing radiation damage (3...is within the range ofsedimentation...biologically significant dose range. Unfortu...

Kevin M. Ulmer; Reinaldo F. Gomez; Anthony J. Sinskey

1979-05-01T23:59:59.000Z

12

Quantitative, non-invasive imaging of radiation-induced DNA double strand breaks in vivo  

NLE Websites -- All DOE Office Websites (Extended Search)

Quantitative, non-invasive imaging of radiation-induced DNA double strand breaks in vivo Quantitative, non-invasive imaging of radiation-induced DNA double strand breaks in vivo Wenrong Li 1, , Fang Li 1 , Qian Huang 1 , Jingping Shen 1 , Frank Wolf 1 , Yujun He 1 , Xinjian Liu 1 , Y. Angela Hu 1 , Joel. S. Bedford 5 , and Chuan-Yuan Li 1,2,* Departments of 1 Radiation Oncology, 2 Pharmacology, University of Colorado School of Medicine, Aurora, Colorado, USA; 3 Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, CO, USA DNA double strand breaks are a major form of DNA damage and a key mechanism through which radiotherapy and some chemotherapeutic agents kill cancer cells. Despite its importance, measuring DNA double strand breaks is still a tedious task that is normally carried out by gel electrophoresis or immunofluorescence staining. Here we report a novel approach to image and

13

Telomeres and DNA double-strand breaks: ever the twain shall S. M. Baileya,* and M. N. Cornforthb  

E-Print Network (OSTI)

between the naturally occurring DNA double-strand breaks (DSBs) found at chromosome ends (i.e., telomeres Collins, CO 80523-1618 (USA), Fax: +19704917742, e-mail: sbailey@colostate.edu b Department of Radiation on their inability to rejoin with broken chromosome ends produced by radiation. Today, we recognize two essential

14

Probability of double-strand breaks in genome-sized DNA by {gamma}-ray decreases markedly as the DNA concentration increases  

SciTech Connect

By use of the single-molecule observation, we count the number of DNA double-strand breaks caused by {gamma}-ray irradiation with genome-sized DNA molecules (166 kbp). We find that P{sub 1}, the number of double-strand breaks (DSBs) per base pair per unit Gy, is nearly inversely proportional to the DNA concentration above a certain threshold DNA concentration. The inverse relationship implies that the total number of DSBs remains essentially constant. We give a theoretical interpretation of our experimental results in terms of attack of reactive species upon DNA molecules, indicating the significance of the characteristics of genome-sized giant DNA as semiflexible polymers for the efficiency of DSBs.

Shimobayashi, Shunsuke F. [Department of Physics, Graduate School of Science, Kyoto University, Kyoto 606-8502 (Japan); Iwaki, Takafumi [Fukui Institute for Fundamental Chemistry, Kyoto University, Kyoto 606-8103 (Japan); Mori, Toshiaki [Radiation Research Center, Osaka Prefecture University, Sakai 599-8570 (Japan); Yoshikawa, Kenichi [Department of Physics, Graduate School of Science, Kyoto University, Kyoto 606-8502 (Japan); Faculty of Life and Medical Sciences, Doshisha University, Kyoto 610-0394 (Japan)

2013-05-07T23:59:59.000Z

15

Induction and Rejoining of DNA Double Strand Breaks Assessed by H2AX Phosphorylation in Melanoma Cells Irradiated with Proton and Lithium Beams  

SciTech Connect

Purpose: The aim of this study was to evaluate the induction and rejoining of DNA double strand breaks (DSBs) in melanoma cells exposed to low and high linear energy transfer (LET) radiation. Methods and Materials: DSBs and survival were determined as a function of dose in melanoma cells (B16-F0) irradiated with monoenergetic proton and lithium beams and with a gamma source. Survival curves were obtained by clonogenic assay and fitted to the linear-quadratic model. DSBs were evaluated by the detection of phosphorylated histone H2AX ({gamma}H2AX) foci at 30 min and 6 h post-irradiation. Results: Survival curves showed the increasing effectiveness of radiation as a function of LET. {gamma}H2AX labeling showed an increase in the number of foci vs. dose for all the radiations evaluated. A decrease in the number of foci was found at 6 h post-irradiation for low LET radiation, revealing the repair capacity of DSBs. An increase in the size of {gamma}H2AX foci in cells irradiated with lithium beams was found, as compared with gamma and proton irradiations, which could be attributed to the clusters of DSBs induced by high LET radiation. Foci size increased at 6 h post-irradiation for lithium and proton irradiations in relation with persistent DSBs, showing a correlation with surviving fraction. Conclusions: Our results showed the response of B16-F0 cells to charged particle beams evaluated by the detection of {gamma}H2AX foci. We conclude that {gamma}H2AX foci size is an accurate parameter to correlate the rejoining of DSBs induced by different LET radiations and radiosensitivity.

Ibanez, Irene L. [Comision Nacional de Energia Atomica, San Martin (Argentina); Consejo Nacional de Investigaciones Cientificas y Tecnicas, Buenos Aires (Argentina); Bracalente, Candelaria [Comision Nacional de Energia Atomica, San Martin (Argentina); Molinari, Beatriz L. [Comision Nacional de Energia Atomica, San Martin (Argentina); Consejo Nacional de Investigaciones Cientificas y Tecnicas, Buenos Aires (Argentina); Palmieri, Monica A. [Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires (Argentina); Policastro, Lucia [Comision Nacional de Energia Atomica, San Martin (Argentina); Consejo Nacional de Investigaciones Cientificas y Tecnicas, Buenos Aires (Argentina); Kreiner, Andres J. [Comision Nacional de Energia Atomica, San Martin (Argentina); Consejo Nacional de Investigaciones Cientificas y Tecnicas, Buenos Aires (Argentina); Escuela de Ciencia y Tecnologia, Universidad Nacional de San Martin (Argentina); Burlon, Alejandro A. [Comision Nacional de Energia Atomica, San Martin (Argentina); Escuela de Ciencia y Tecnologia, Universidad Nacional de San Martin (Argentina); Valda, Alejandro [Escuela de Ciencia y Tecnologia, Universidad Nacional de San Martin (Argentina); Navalesi, Daniela [Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires (Argentina); Davidson, Jorge; Davidson, Miguel [Consejo Nacional de Investigaciones Cientificas y Tecnicas, Buenos Aires (Argentina); Vazquez, Monica; Ozafran, Mabel [Comision Nacional de Energia Atomica, San Martin (Argentina); Duran, Hebe [Comision Nacional de Energia Atomica, San Martin (Argentina); Consejo Nacional de Investigaciones Cientificas y Tecnicas, Buenos Aires (Argentina); Escuela de Ciencia y Tecnologia, Universidad Nacional de San Martin (Argentina)], E-mail: hduran@cnea.gov.ar

2009-07-15T23:59:59.000Z

16

Creating Directed Double-strand Breaks with the Ref Protein: A Novel Rec A-Dependent Nuclease from Bacteriophage P1  

SciTech Connect

The bacteriophage P1-encoded Ref protein enhances RecA-dependent recombination in vivo by an unknown mechanism. We demonstrate that Ref is a new type of enzyme; that is, a RecA-dependent nuclease. Ref binds to ss- and dsDNA but does not cleave any DNA substrate until RecA protein and ATP are added to form RecA nucleoprotein filaments. Ref cleaves only where RecA protein is bound. RecA functions as a co-nuclease in the Ref/RecA system. Ref nuclease activity can be limited to the targeted strands of short RecA-containing D-loops. The result is a uniquely programmable endonuclease activity, producing targeted double-strand breaks at any chosen DNA sequence in an oligonucleotide-directed fashion. We present evidence indicating that cleavage occurs in the RecA filament groove. The structure of the Ref protein has been determined to 1.4 {angstrom} resolution. The core structure, consisting of residues 77-186, consists of a central 2-stranded {beta}-hairpin that is sandwiched between several {alpha}-helical and extended loop elements. The N-terminal 76 amino acid residues are disordered; this flexible region is required for optimal activity. The overall structure of Ref, including several putative active site histidine residues, defines a new subclass of HNH-family nucleases. We propose that enhancement of recombination by Ref reflects the introduction of directed, recombinogenic double-strand breaks.

Gruenig, Marielle C.; Lu, Duo; Won, Sang Joon; Dulberger, Charles L.; Manlick, Angela J.; Keck, James L.; Cox, Michael M. (UW)

2012-03-16T23:59:59.000Z

17

Induction of DNA Double-Strand Breaks and Cellular Migration Through Bystander Effects in Cells Irradiated With the Slit-Type Microplanar Beam of the Spring-8 Synchrotron  

SciTech Connect

Purpose: To determine whether glioma cells irradiated with a microplanar X-ray beam exert bystander effects. Methods and Materials: Microplanar beam irradiation of glioma cells in vitro was done using the SPring-8 synchrotron radiation facility. The amount of DNA double-strand breaks (dsbs) was measured by the fluorescence intensity of phosphorylated H2AX or the number of 53BP1 foci. The dose distribution in a cell population exposed to a single microplanar beam was determined by the amount of phosphorylated H2AX-positive cells. Bystander effects were determined by counting the number of 53BP1 foci in nonirradiated cells treated with conditioned medium from cultures of irradiated cells. Results: More DNA dsbs were detected in cells adjacent to an area irradiated by the single beam than in cells in distant, nonirradiated areas as a result of bystander effects caused by scattered X-rays and DNA dsbs. In support of this, more 53BP1 foci were observed in nonirradiated, conditioned medium-treated cells than in control cells (i.e., cells not treated with irradiation or conditioned medium). These results suggest that DNA dsbs were induced in nonirradiated cells by soluble factors in the culture medium. In addition, we observed cellular migration into areas irradiated with peak doses, suggesting that irradiated cells send signals that cause nonirradiated cells to migrate toward damaged cells. Conclusions: Bystander effects are produced by factors secreted as a result of slit-type microplanar X-ray beam irradiation.

Kashino, Genro [Research Reactor Institute, Kyoto University, Osaka (Japan)], E-mail: kashino@rri.kyoto-u.ac.jp; Kondoh, Takeshi [Department of Neurosurgery, Graduate School of Medicine, Kobe University, Kobe (Japan); Nariyama, Nobuteru; Umetani, Keiji; Ohigashi, Takuji [Japan Synchrotron Radiation Research Institute, Hyogo (Japan); Shinohara, Kunio [Japan Synchrotron Radiation Research Institute, Hyogo (Japan); Advanced Research Institute for Science and Engineering, Waseda University, Tokyo (Japan); Kurihara, Ai; Fukumoto, Manabu [Department of Pathology, Institute of Development, Aging and Cancer, Tohoku University, Sendai (Japan); Tanaka, Hiroki; Maruhashi, Akira; Suzuki, Minoru; Kinashi, Yuko; Liu Yong; Masunaga, Shin-ichiro; Watanabe, Masami; Ono, Koji [Research Reactor Institute, Kyoto University, Osaka (Japan)

2009-05-01T23:59:59.000Z

18

PAXX, a paralog of XRCC4 and XLF, interacts with Ku to promote DNA double-strand break repair  

E-Print Network (OSTI)

regions of PAXX1-204 and PAXXV199A/F201A were amplified from the vectors by PCR and cloned into the pcDNA3.1(-) vector (a gift from Dr. V. Bolanos-Garcia) together with GFP or FLAG tags using In-Fusion (Clontech). Vectors were sequenced by the DNA... -treated or treated for 1 hour with 300 µM phleomycin to induce large numbers of DSBs, before being harvested either at this point or after an additional 1-hour recovery period in phleomycin-free medium after three PBS washes. Cells were washed twice in ice-cold PBS...

Ochi, Takashi; Blackford, Andrew N.; Coates, Julia; Jhujh, Satpal; Mehmood, Shahid; Tamura, Naoka; Travers, Jon; Wu, Qian; Draviam, Viji M.; Robinson, Carol V.; Blundell, Tom L.; Jackson, Stephen P.

2015-01-09T23:59:59.000Z

19

Functional role of MRE11 in the repair of particulate chromium(VI)-induced DNA double strand breaks  

Science Journals Connector (OSTI)

...mast cells can behave alternatively as dangerous promoters, innocent bystanders, or...Italo Monzino, the Italian Ministry of Health, and Associazione Italiana Ricerca sul...mast cells can behave alternatively as dangerous promoters, innocent bystanders, or...

Hong Xie; Sandra S. Wise; Seme Dako; Spiros Katsifis; and John P. Wise

2006-04-15T23:59:59.000Z

20

Low Dose Radiation Research Program: Quantification of Repair of  

NLE Websites -- All DOE Office Websites (Extended Search)

Quantification of Repair of Low-Dose-Induced DNA Double-Strand Quantification of Repair of Low-Dose-Induced DNA Double-Strand Breaks in Diploid Human Cells Authors: David Schild,1 and Larry H. Thompson,2 Institutions: 1Life Sciences Division, Lawrence Berkeley National Laboratory; and 2BBR Program, Lawrence Livermore National Laboratory Double-strand breaks (DSBs) are the biochemical lesions of primary concern in radiation related health effects. Compelling evidence from rodent and chicken model systems indicates that homologous recombinational repair (HRR) plays an essential role for cell viability in the repair of spontaneous DSBs arising during DNA replication and an important role in the repair of IR-induced DSBs. IR-induced DSBs are also repaired by error-prone nonhomologous end joining (NHEJ). Using hTERT-immortalized

Note: This page contains sample records for the topic "double-strand break repair" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


21

Proteogenomic Analysis Reveals Unanticipated Adaptations of Colorectal Tumor Cells to Deficiencies in DNA Mismatch Repair  

Science Journals Connector (OSTI)

...related double-strand break repair protein NBN, which are constituents...decreased DNA synthesis and repair in MMR- cells (Fig. 4...application of our newly developed pipeline for the detection of variant...in DNA and RNA synthesis and repair. The driving proteins behind...

Patrick J. Halvey; Xiaojing Wang; Jing Wang; Ajaz A. Bhat; Punita Dhawan; Ming Li; Bing Zhang; Daniel C. Liebler; and Robbert J.C. Slebos

2014-01-01T23:59:59.000Z

22

Nej1 recruits the Srs2 helicase to DNA double-strand breaks and supports repair by a single-strand annealing-like mechanism  

Science Journals Connector (OSTI)

...strain and its derivatives. Relative to input DNA, galactose-induction in the SRS2-13XMYC WT strain...performed as described in ref. 52. Input DNA was recovered before immunoprecipitation...Genetic screens and selections for cell and nuclear fusion mutants . Methods Enzymol 194 : 774...

Sidney D. Carter; Dana Vigašová; Jiang Chen; Miroslav Chovanec; Stefan U. Åström

2009-01-01T23:59:59.000Z

23

Identification, Exploitation and Manipulation of BRCA1-Dependent DNA Double-Strand Break and Interstrand Crosslink Repair in Breast and Ovarian Cancer Therapy  

E-Print Network (OSTI)

Expression of functional breast cancer susceptibility gene 1 (BRCA1) in human cancers is associated with resistance to platinum-based chemotherapeutics and poly(ADP-ribose) polymerase (PARP) inhibitors. BRCA1 is a nuclear phosphoprotein with broad...

Stecklein, Shane Richard

2012-05-12T23:59:59.000Z

24

RAD-51-Dependent and -Independent Roles of a Caenorhabditis elegans BRCA2-Related Protein during DNA Double-Strand Break Repair  

Science Journals Connector (OSTI)

...previously described (5). To test pair-wise interactions in yeast cells, Gal4 DNA...four BRCs) (11, 21, 41, 49). Pair-wise alignment of the single BRC of T07E3...In addition, we thank Juliet Reid, Alison Schuldt, John Diffley, Jesper Svejstrup...

Julie S. Martin; Nicole Winkelmann; Mark I. R. Petalcorin; Michael J. McIlwraith; Simon J. Boulton

2005-04-01T23:59:59.000Z

25

Synergistic Anticancer Activity of Arsenic Trioxide with Erlotinib Is Based on Inhibition of EGFR-Mediated DNA Double-Strand Break Repair  

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...erlotinib (B). C, the diagrams on the left show viability...molecular mechanisms. Materials and Methods Chemicals...camera. Subsequent image handling was carried out in Adobe...in vivo. A schematic diagram illustrating the molecular...Administrative, technical, or material support (i.e...

Kushtrim Kryeziu; Ute Jungwirth; Mir Alireza Hoda; Franziska Ferk; Siegfried Knasmüller; Claudia Karnthaler-Benbakka; Christian R. Kowol; Walter Berger; and Petra Heffeter

2013-06-01T23:59:59.000Z

26

Increased apoptosis and DNA double-strand breaks in the embryonic mouse brain in response to very low-dose X-rays but not 50 Hz magnetic fields  

Science Journals Connector (OSTI)

...micronucleus formation, mutation induction and single-strand break...generated using a function generator and power amplifier connected...Assuming the same level of DSB induction, we conclude that the difference...low-dose radiation To assess the induction of 53BP1 foci after low-dose...

2014-01-01T23:59:59.000Z

27

Lattice ribbons: A model of double-stranded polymers  

Science Journals Connector (OSTI)

We introduce a discrete ribbon model for double-stranded polymers (such as duplex DNA) where the ribbon is constrained to lie on the simple cubic lattice Z3. The ribbon is made up of a sequence of plaquettes and can either be open or closed. We investigate the growth of the number of ribbons as a function of the number of plaquettes and use Monte Carlo methods to estimate the dimensions of the ribbon, the writhe of the backbone and, in the case of orientable closed ribbons, the linking number of the two boundary curves.

E.J. Janse van Rensburg; E. Orlandini; D.W Sumners; M.C. Tesi; S.G. Whittington

1994-12-01T23:59:59.000Z

28

The effect of double-stranded (DS) RNA on Monosporascus cannonballus culture morphology and virulence  

E-Print Network (OSTI)

Attempts to eliminate double-stranded (ds) RNA elements in Monosporascus cannonballus by subculturing on PDA amended with various concentrations of cycloheximide (O to 15 pg/ml) and incubating at elevated temperatures were partially effective...

Park, Yunjung

2012-06-07T23:59:59.000Z

29

Single-Molecule Measurements of the Persistence Length of Double-Stranded RNA  

E-Print Network (OSTI)

Single-Molecule Measurements of the Persistence Length of Double-Stranded RNA J. A. Abels, FRNA using two different single-molecule techniques: magnetic tweezers and atomic force microscopy. We deduce. The introduction of these single-molecule techniques for dsRNA analysis opens the way for real-time, quantitative

Dekker, Nynke

30

Spin transport and spin polarization properties in double-stranded DNA  

SciTech Connect

We study the spin-dependent electron transport through a double-stranded DNA (dsDNA) using the Bogoliubov-de Gennes equations and non-equilibrium Green's function method. We calculate the spin-dependent electron conductance and spin-polarization for different lengths, helix angles, twist angles of dsDNA, the environment-induced dephasing factors, and hopping integral. It is shown that the conductance decreases by increasing the length and dephasing factor. Also, we show that the spin-polarization depends on the helical symmetry and the length of DNA. It is shown that the double-stranded DNA can act as a perfect spin filter. Finally, we show that the sign of spin polarization can be inverted from +1 (?1) to ?1 (+1) for some values of hopping integral.

Simchi, Hamidreza, E-mail: simchi@iust.ac.ir [Department of Physics, Iran University of Science and Technology, Narrmak, Tehran 16844 (Iran, Islamic Republic of); Semiconductor Technology Center, Tehran (Iran, Islamic Republic of); Esmaeilzadeh, Mahdi, E-mail: mahdi@iust.ac.ir; Mazidabadi, Hossein [Department of Physics, Iran University of Science and Technology, Narrmak, Tehran 16844 (Iran, Islamic Republic of)

2013-11-21T23:59:59.000Z

31

Low Dose Radiation Induced DNA Damage Signaling and Repair Responses in  

NLE Websites -- All DOE Office Websites (Extended Search)

Induced DNA Damage Signaling and Repair Responses in Induced DNA Damage Signaling and Repair Responses in Human 3-Dimensional Skin Model System Yanrong Su, Jarah Meador and Adayabalam S. Balajee Center for Radiological Research, College of Physicians and Surgeons, Columbia University, 630 West, 168th Street, New York, NY 10032. Exposure to ionizing radiation (IR) inflicts a wide variety of lesions in the genomic DNA. Among them, DNA double strand break (DSB) is considered to be the critical lesion for most of the deleterious radiation effects including carcinogenesis. Much of our knowledge on induction and repair kinetics of DSB has come from studies in two dimensional cell culture systems. However, the damage signaling and repair responses to DSB in tissue microenvironment are largely unknown. Knowledge of tissue responses to

32

Examining the regulation of DNA end-processing at telomeres and double-strand breaks  

E-Print Network (OSTI)

do not exhibit synthetic growth or DNA damage responsedo not exhibit synthetic growth or DNA damage responsedo not exhibit synthetic growth or DNA damage response

Ballew, Bari Jane

2011-01-01T23:59:59.000Z

33

Evidence for the Involvement of Double-Strand Breaks in Heat-Induced Cell Killing  

Science Journals Connector (OSTI)

...between thermal tolerance for heat killing and heat-induced loss of DNA polymerase b activity...cells with different cell cycle distributions. Error bars, SD. Fig. 5...between thermotolerance and heat-induced H2AX foci formation...

Akihisa Takahashi; Hideki Matsumoto; Kosuke Nagayama; Mutsuko Kitano; Sayako Hirose; Hidenori Tanaka; Eiichiro Mori; Nobuhiro Yamakawa; Jun-ichi Yasumoto; Kazue Yuki; Ken Ohnishi; and Takeo Ohnishi

2004-12-15T23:59:59.000Z

34

Induction of linear tracks of DNA double-strand breaks by -particle irradiation of  

E-Print Network (OSTI)

eukaryotic cells by exposing the cells to particles from a small Americium source (Box 1). Each particle) EQUIPMENT Americium (Am-241) source (SupplementaryMethods and SupplementaryFigs.1­3 online) Polyester film

Cai, Long

35

A MICROBEAM STUDY OF DNA DOUBLE-STRAND BREAKS IN BYSTANDER PRIMARY HUMAN FIBROBLASTS  

E-Print Network (OSTI)

-or-nothing manner(7) . Bystander cells exhibit a variety of characteristics of IR-induced genomic instability

36

Exotic quantum transport in double-stranded Kronig-Penney model  

E-Print Network (OSTI)

We introduce double-stranded Kronig-Penney model and analyze its transport properties. The asymmetric fluxes between two strands with suddenly alternating localization patterns are found as the energy is varied. The zero-size limit of the internal lines connecting two strands is examined using quantum graph vertices with four edges. We also consider a two-dimensional Kronig-Penney lattice with two types of alternating layers with $\\delta$ and $\\delta'$ connections, and show that the existence of energy bands in which the quantum flux can flow only in selected directions.

Taksu Cheon; Sergey S. Poghosyan

2014-10-31T23:59:59.000Z

37

Proximity-induced superconductivity effect in a double-stranded DNA  

SciTech Connect

We study the proximity-induced superconductivity effect in a double-stranded DNA by solving the Bogoliubov-de Gennes equations and taking into account the effect of thermal fluctuations of the twist angle between neighboring base pairs. We show that the electron conductance is spin-dependent and the conductance of spin up (down) increases (decreases) due to the spin-orbit coupling (SOC). It is found that, for T?

Simchi, Hamidreza, E-mail: simchi@iust.ac.ir [Department of Physics, Iran University of Science and Technology, Narrmak, Tehran 16844 (Iran, Islamic Republic of); Semiconductor Technology Center, Tehran (Iran, Islamic Republic of); Esmaeilzadeh, Mahdi, E-mail: mahdi@iust.ac.ir; Mazidabadi, Hossein [Department of Physics, Iran University of Science and Technology, Narrmak, Tehran 16844 (Iran, Islamic Republic of)

2014-02-07T23:59:59.000Z

38

Low Dose Radiation Research Program: Checkpoint Responses and Repair of a  

NLE Websites -- All DOE Office Websites (Extended Search)

Checkpoint Responses and Repair of a Broken Chromosome in Budding Checkpoint Responses and Repair of a Broken Chromosome in Budding Yeast. Authors: André Walther, Mreshwar B. Vaze, Anna Malkova, Grzegorz Ira, Xuan Wang, Jung-Ae Kim, Neal Sugawara, James E. Haber Brandeis University, Waltham, Massachusetts We have studied how yeast cells respond to the creation of a single double-strand break (DSB) by using a site-specific endonuclease to create one (or more) DSBs. In collaboration with R. Schroff, M. Licheten and W. Bonner (NIH) we have shown that a single DSB rapidly becomes associated with histone H2AX-phosphorylation (y-H2AX) covering a distance of about 25 kb on either side of the DSB. The drop-off in phosphorylation is not attributable to specific boundary sequences. y-H2AX can be phosphorylated by either ATR (Mec1) or ATM (Tel1) protein kinases. A mutation that removes

39

KUB5/HERA: a dual acting protein that suppresses genomic instability and promotes DNA repair after low dose IR exposure  

NLE Websites -- All DOE Office Websites (Extended Search)

KUB5/HERA: a dual acting protein that suppresses genomic instability and KUB5/HERA: a dual acting protein that suppresses genomic instability and promotes DNA repair after low dose IR exposure Julio C. Morales 1 , Amy Rommel 1 , Konstantin Leskov 2 , Walter M. Hittelman 3 , David A. Boothman 1# 1 Simmons Cancer Center, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA. 2 Department of Radiation Oncology, Case Western Reserve University, Cleveland, OH 44106, USA. 3 Department of Experimental Therapeutics, M.D. Anderson Cancer Center, Houston, TX 77030, USA. # To whom correspondence should be addressed. E-mail: David.Boothman@utsouthwestern.edu Eukaryotic cells can respond to DNA double strand breaks created by low doses of IR by activating homologous recombination (HR) or non-homologous end- joining (NHEJ) pathways to repair DNA. A yeast two-hybrid screen using Ku70 as

40

Activation of double-stranded RNA-dependent protein kinase inhibits proliferation of pancreatic ?-cells  

SciTech Connect

Highlights: •PKR can be activated by glucolipitoxicity and pro-inflammatory cytokines in ?-cells. •Activated PKR inhibited ?-cell proliferation by arresting cell cycle at G1 phase. •Activated PKR fully abrogated the pro-proliferative effects of IGF-I on ?-cells. -- Abstract: Double-stranded RNA-dependent protein kinase (PKR) is revealed to participate in the development of insulin resistance in peripheral tissues in type 2 diabetes (T2DM). Meanwhile, PKR is also characterized as a critical regulator of cell proliferation. To date, no study has focused on the impact of PKR on the proliferation of pancreatic ?-cells. Here, we adopted insulinoma cell lines and mice islet ?-cells to investigate: (1) the effects of glucolipotoxicity and pro-inflammatory cytokines on PKR activation; (2) the effects of PKR on proliferation of pancreatic ?-cells and its underlying mechanisms; (3) the actions of PKR on pro-proliferative effects of IGF-I and its underlying pathway. Our results provided the first evidence that PKR can be activated by glucolipitoxicity and pro-inflammatory cytokines in pancreatic ?-cells, and activated PKR significantly inhibited cell proliferation by arresting cell cycle at G1 phase. Reductions in cyclin D1 and D2 as well as increases in p27 and p53 were associated with the anti-proliferative effects of PKR, and proteasome-dependent degradation took part in the reduction of cyclin D1 and D2. Besides, PKR activation abrogated the pro-proliferative effects of IGF-I by activating JNK and disrupting IRS1/PI3K/Akt signaling pathway. These findings indicate that the anti-proliferative actions of PKR on pancreatic ?-cells may contribute to the pathogenesis of T2DM.

Chen, Shan-Shan [Key Laboratory of Human Functional Genomics of Jiangsu Province, Nanjing Medical University, Nanjing (China) [Key Laboratory of Human Functional Genomics of Jiangsu Province, Nanjing Medical University, Nanjing (China); Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing (China); Jiang, Teng [Department of Neurology, Qingdao Municipal Hospital, Nanjing Medical University, Nanjing (China)] [Department of Neurology, Qingdao Municipal Hospital, Nanjing Medical University, Nanjing (China); Wang, Yi; Gu, Li-Ze [Key Laboratory of Human Functional Genomics of Jiangsu Province, Nanjing Medical University, Nanjing (China) [Key Laboratory of Human Functional Genomics of Jiangsu Province, Nanjing Medical University, Nanjing (China); Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing (China); Wu, Hui-Wen [Laboratory Center for Basic Medical Sciences, Nanjing Medical University, Nanjing (China)] [Laboratory Center for Basic Medical Sciences, Nanjing Medical University, Nanjing (China); Tan, Lan [Department of Neurology, Qingdao Municipal Hospital, Nanjing Medical University, Nanjing (China)] [Department of Neurology, Qingdao Municipal Hospital, Nanjing Medical University, Nanjing (China); Guo, Jun, E-mail: Guoj@njmu.edu.cn [Key Laboratory of Human Functional Genomics of Jiangsu Province, Nanjing Medical University, Nanjing (China) [Key Laboratory of Human Functional Genomics of Jiangsu Province, Nanjing Medical University, Nanjing (China); Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing (China)

2014-01-17T23:59:59.000Z

Note: This page contains sample records for the topic "double-strand break repair" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


41

DNA double-strand breaks promote methylation of histone H3 on lysine 9 and transient formation of repressive chromatin  

Science Journals Connector (OSTI)

...7456 ): 50 – 54 . 11 Price BD D’Andrea AD ( 2013...Y Gursoy-Yuzugullu O Price BD ( 2012 ) The...Apochromat/1.25-N.A. oil objective (Leica...Plan APO 63×/1.4 oil M27 lens (magnification...proteins were released by heating for 5 min with 2× sample...

Marina K. Ayrapetov; Ozge Gursoy-Yuzugullu; Chang Xu; Ye Xu; Brendan D. Price

2014-01-01T23:59:59.000Z

42

DNA-Repair Genetic Polymorphisms and Breast Cancer Risk  

Science Journals Connector (OSTI)

...auspices of US Department of Energy Contract #W-7405-ENG-48...double-strand break; FH, family history; HWE, Hardy-Weinberg...R., Perry H. R., Price F. M., Sanford K...adjustment for age, family history, age at menarche, age...North Carolina 27157, USA. | Journal Article Research...

Tasha R. Smith; Edward A. Levine; Nancy D. Perrier; Mark Steven Miller; Rita I. Freimanis; Kurt Lohman; L. Douglas Case; Jianfeng Xu; Harvey W. Mohrenweiser; and Jennifer J. Hu

2003-11-01T23:59:59.000Z

43

Chemical repair of base lesions, AP-sites, and strand breaks on plasmid DNA in dilute aqueous solution by ascorbic acid  

SciTech Connect

Highlights: •We report a novel mechanism of radiation protection of DNA by chemical activity of ascorbic acid. •The “chemical repair” of DNA damage was revealed using biochemical assay and chemical kinetics analysis. •We found that ascorbic acid significantly repairs precursors of nucleobase lesions and abasic sites. •However, ascorbic acid seldom repairs precursors of DNA-strand breaks. -- Abstract: We quantified the damage yields produced in plasmid DNA by ?-irradiation in the presence of low concentrations (10–100 ?M) of ascorbic acid, which is a major antioxidant in living systems, to clarify whether it chemically repairs radiation damage in DNA. The yield of DNA single strand breaks induced by irradiation was analyzed with agarose gel electrophoresis as conformational changes in closed circular plasmids. Base lesions and abasic sites were also observed as additional conformational changes by treating irradiated samples with glycosylase proteins. By comparing the suppression efficiencies to the induction of each DNA lesion, in addition to scavenging of the OH radicals derived from water radiolysis, it was found that ascorbic acid promotes the chemical repair of precursors of AP-sites and base lesions more effectively than those of single strand breaks. We estimated the efficiency of the chemical repair of each lesion using a kinetic model. Approximately 50–60% of base lesions and AP-sites were repaired by 10 ?M ascorbic acid, although strand breaks were largely unrepaired by ascorbic acid at low concentrations. The methods in this study will provide a route to understanding the mechanistic aspects of antioxidant activity in living systems.

Hata, Kuniki [Department of Nuclear Engineering and Management, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656 (Japan) [Department of Nuclear Engineering and Management, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656 (Japan); Advanced Science Research Center, Japan Atomic Energy Agency, 2-4 Shirakatashirane, Tokai-mura, Naka-gun, Ibaraki 319-1195 (Japan); Urushibara, Ayumi; Yamashita, Shinichi; Shikazono, Naoya; Yokoya, Akinari [Advanced Science Research Center, Japan Atomic Energy Agency, 2-4 Shirakatashirane, Tokai-mura, Naka-gun, Ibaraki 319-1195 (Japan)] [Advanced Science Research Center, Japan Atomic Energy Agency, 2-4 Shirakatashirane, Tokai-mura, Naka-gun, Ibaraki 319-1195 (Japan); Katsumura, Yosuke, E-mail: katsu@n.t.u-tokyo.ac.jp [Department of Nuclear Engineering and Management, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656 (Japan) [Department of Nuclear Engineering and Management, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656 (Japan); Nuclear Professional School, School of Engineering, The University of Tokyo, 2-22 Shirakatashirane, Tokai-mura, Naka-gun, Ibaraki 319-1188 (Japan)

2013-05-03T23:59:59.000Z

44

DNA repair: Dynamic defenders against cancer and aging  

SciTech Connect

You probably weren't thinking about your body's cellular DNA repair systems the last time you sat on the beach in the bright sunshine. Fortunately, however, while you were subjecting your DNA to the harmful effects of ultraviolet light, your cells were busy repairing the damage. The idea that our genetic material could be damaged by the sun was not appreciated in the early days of molecular biology. When Watson and Crick discovered the structure of DNA in 1953 [1], it was assumed that DNA is fundamentally stable since it carries the blueprint of life. However, over 50 years of research have revealed that our DNA is under constant assault by sunlight, oxygen, radiation, various chemicals, and even our own cellular processes. Cleverly, evolution has provided our cells with a diverse set of tools to repair the damage that Mother Nature causes. DNA repair processes restore the normal nucleotide sequence and DNA structure of the genome after damage [2]. These responses are highly varied and exquisitely regulated. DNA repair mechanisms are traditionally characterized by the type of damage repaired. A large variety of chemical modifications can alter normal DNA bases and either lead to mutations or block transcription if not repaired, and three distinct pathways exist to remove base damage. Base excision repair (BER) corrects DNA base alterations that do not distort the overall structure of the DNA helix such as bases damaged by oxidation resulting from normal cellular metabolism. While BER removes single damaged bases, nucleotide excision repair (NER) removes short segments of nucleotides (called oligonucleotides) containing damaged bases. NER responds to any alteration that distorts the DNA helix and is the mechanism responsible for repairing bulky base damage caused by carcinogenic chemicals such as benzo [a]pyrene (found in cigarette smoke and automobile exhaust) as well as covalent linkages between adjacent pyrimidine bases resulting from the ultraviolet (UV) component of sunlight. NER can be divided into two classes based on where the repair occurs. NER occurring in DNA that is not undergoing transcription (i.e., most of the genome) is called global genome repair (GGR or GGNER), while NER taking place in the transcribed strand of active genes is called transcription-coupled repair (TCR or TC-NER). We will explore NER in more detail below. Mismatch repair (MMR) is another type of excision repair that specifically removes mispaired bases resulting from replication errors. DNA damage can also result in breaks in the DNA backbone, in one or both strands. Single-strand breaks (SSBs) are efficiently repaired by a mechanism that shares common features with the later steps in BER. Double-strand breaks (DSBs) are especially devastating since by definition there is no intact complementary strand to serve as a template for repair, and even one unrepaired DSB can be lethal [3]. In cells that have replicated their DNA prior to cell division, the missing information can be supplied by the duplicate copy, or sister chromatid, and DSBs in these cells are faithfully repaired by homologous recombination involving the exchange of strands of DNA between the two copies. However, most cells in the body are non-dividing, and in these cells the major mechanism for repairing DSBs is by non-homologous end joining (NHEJ), which as the name implies involves joining two broken DNA ends together without a requirement for homologous sequence and which therefore has a high potential for loss of genetic information.

Fuss, Jill O.; Cooper, Priscilla K.

2006-04-01T23:59:59.000Z

45

Mutations Abrogating VP35 Interaction with Double-Stranded RNA Render Ebola Virus Avirulent in Guinea Pigs  

SciTech Connect

Ebola virus (EBOV) protein VP35 is a double-stranded RNA (dsRNA) binding inhibitor of host interferon (IFN)-{alpha}/{beta} responses that also functions as a viral polymerase cofactor. Recent structural studies identified key features, including a central basic patch, required for VP35 dsRNA binding activity. To address the functional significance of these VP35 structural features for EBOV replication and pathogenesis, two point mutations, K319A/R322A, that abrogate VP35 dsRNA binding activity and severely impair its suppression of IFN-{alpha}/{beta} production were identified. Solution nuclear magnetic resonance (NMR) spectroscopy and X-ray crystallography reveal minimal structural perturbations in the K319A/R322A VP35 double mutant and suggest that loss of basic charge leads to altered function. Recombinant EBOVs encoding the mutant VP35 exhibit, relative to wild-type VP35 viruses, minimal growth attenuation in IFN-defective Vero cells but severe impairment in IFN-competent cells. In guinea pigs, the VP35 mutant virus revealed a complete loss of virulence. Strikingly, the VP35 mutant virus effectively immunized animals against subsequent wild-type EBOV challenge. These in vivo studies, using recombinant EBOV viruses, combined with the accompanying biochemical and structural analyses directly correlate VP35 dsRNA binding and IFN inhibition functions with viral pathogenesis. Moreover, these studies provide a framework for the development of antivirals targeting this critical EBOV virulence factor.

Prins, Kathleen C.; Delpeut, Sebastien; Leung, Daisy W.; Reynard, Olivier; Volchkova, Valentina A.; Reid, St. Patrick; Ramanan, Parameshwaran; Cárdenas, Washington B.; Amarasinghe, Gaya K.; Volchkov, Viktor E.; Basler, Christopher F. (CNRS-INSERM); (Mount Sinai Hospital); (LB-Ecuador); (Iowa State)

2010-10-11T23:59:59.000Z

46

Breaking  

NLE Websites -- All DOE Office Websites (Extended Search)

2 Volume 9 Issue 3 2 Volume 9 Issue 3 Closer kin for reference . . . . . . . . . . . . . . . . . . . . . . . 2 Breaking down pulp . . . . . . . . . . . . . . . . . . . . . . . . . 3 Single-cell genomics at the DOE JGI . . . . . . . . . . . . 4 In the news . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-7 also in this issue MASSIE SANTOS BALLON The Department of Energy focuses on a variety of alternative energy sources rather than putting all its eggs in one basket. At the recently concluded 7th annual Sequencing, Finishing, Analysis in the Future (SFAF) Meeting held June 5-7, 2012, a similar message came through as speakers discussed combining sequencing platforms and conducting hybrid assemblies to produce more useful data in the form of finished genome sequences. Since the first Sequencing in the Future Meeting held in Santa Fe, New Mexico seven years ago, the number of attendees has increased each year from a starting group of 70, reaching a

47

The impact of homologous recombination repair deficiency on depleted uranium clastogenicity in Chinese hamster ovary cells: XRCC3 protects cells from chromosome aberrations, but increases chromosome fragmentation  

Science Journals Connector (OSTI)

Abstract Depleted uranium (DU) is extensively used in both industry and military applications. The potential for civilian and military personnel exposure to DU is rising, but there are limited data on the potential health hazards of DU exposure. Previous laboratory research indicates DU is a potential carcinogen, but epidemiological studies remain inconclusive. DU is genotoxic, inducing DNA double strand breaks, chromosome damage and mutations, but the mechanisms of genotoxicity or repair pathways involved in protecting cells against DU-induced damage remain unknown. The purpose of this study was to investigate the effects of homologous recombination repair deficiency on DU-induced genotoxicity using RAD51D and XRCC3-deficient Chinese hamster ovary (CHO) cell lines. Cells deficient in XRCC3 (irs1SF) exhibited similar cytotoxicity after DU exposure compared to wild-type (AA8) and XRCC3-complemented (1SFwt8) cells, but DU induced more break-type and fusion-type lesions in XRCC3-deficient cells compared to wild-type and XRCC3-complemented cells. Surprisingly, loss of RAD51D did not affect DU-induced cytotoxicity or genotoxicity. DU induced selective X-chromosome fragmentation irrespective of RAD51D status, but loss of XRCC3 nearly eliminated fragmentation observed after DU exposure in wild-type and XRCC3-complemented cells. Thus, XRCC3, but not RAD51D, protects cells from DU-induced breaks and fusions and also plays a role in DU-induced chromosome fragmentation.

Amie L. Holmes; Kellie Joyce; Hong Xie; Carolyne Falank; John M. Hinz; John Pierce Wise Sr.

2014-01-01T23:59:59.000Z

48

ATR Affecting Cell Radiosensitivity Is Dependent on Homologous Recombination Repair but Independent of Nonhomologous End Joining  

Science Journals Connector (OSTI)

...Toxicology, Maine Center for Toxicology and Environmental Health, Portland, ME, University of Bridgeport, Bridgeport, CT...DNA double strand breaks (DSBs) formation, one of the most dangerous types of DNA damage. The hMRE11-RAD50-NBS1 protein complex...

Hongyan Wang; Huichen Wang; Simon N. Powell; George Iliakis; and Ya Wang

2004-10-01T23:59:59.000Z

49

Homologous recombination and non-homologous end-joining repair pathways in bovine embryos with different developmental competence  

SciTech Connect

This study investigated the expression of genes controlling homologous recombination (HR), and non-homologous end-joining (NHEJ) DNA-repair pathways in bovine embryos of different developmental potential. It also evaluated whether bovine embryos can respond to DNA double-strand breaks (DSBs) induced with ultraviolet irradiation by regulating expression of genes involved in HR and NHEJ repair pathways. Embryos with high, intermediate or low developmental competence were selected based on the cleavage time after in vitro insemination and were removed from in vitro culture before (36 h), during (72 h) and after (96 h) the expected period of embryonic genome activation. All studied genes were expressed before, during and after the genome activation period regardless the developmental competence of the embryos. Higher mRNA expression of 53BP1 and RAD52 was found before genome activation in embryos with low developmental competence. Expression of 53BP1, RAD51 and KU70 was downregulated at 72 h and upregulated at 168 h post-insemination in response to DSBs induced by ultraviolet irradiation. In conclusion, important genes controlling HR and NHEJ DNA-repair pathways are expressed in bovine embryos, however genes participating in these pathways are only regulated after the period of embryo genome activation in response to ultraviolet-induced DSBs.

Henrique Barreta, Marcos [Universidade Federal de Santa Catarina, Campus Universitario de Curitibanos, Curitibanos, SC (Brazil) [Universidade Federal de Santa Catarina, Campus Universitario de Curitibanos, Curitibanos, SC (Brazil); Laboratorio de Biotecnologia e Reproducao Animal-BioRep, Universidade Federal de Santa Maria, Santa Maria, RS (Brazil); Garziera Gasperin, Bernardo; Braga Rissi, Vitor; Cesaro, Matheus Pedrotti de [Laboratorio de Biotecnologia e Reproducao Animal-BioRep, Universidade Federal de Santa Maria, Santa Maria, RS (Brazil)] [Laboratorio de Biotecnologia e Reproducao Animal-BioRep, Universidade Federal de Santa Maria, Santa Maria, RS (Brazil); Ferreira, Rogerio [Centro de Educacao Superior do Oeste-Universidade do Estado de Santa Catarina, Chapeco, SC (Brazil)] [Centro de Educacao Superior do Oeste-Universidade do Estado de Santa Catarina, Chapeco, SC (Brazil); Oliveira, Joao Francisco de; Goncalves, Paulo Bayard Dias [Laboratorio de Biotecnologia e Reproducao Animal-BioRep, Universidade Federal de Santa Maria, Santa Maria, RS (Brazil)] [Laboratorio de Biotecnologia e Reproducao Animal-BioRep, Universidade Federal de Santa Maria, Santa Maria, RS (Brazil); Bordignon, Vilceu, E-mail: vilceu.bordignon@mcgill.ca [Department of Animal Science, McGill University, Ste-Anne-De-Bellevue, QC (Canada)] [Department of Animal Science, McGill University, Ste-Anne-De-Bellevue, QC (Canada)

2012-10-01T23:59:59.000Z

50

Investigating Deinococcus radiodurans RecA Protein Filament Formation on Double-Stranded DNA by a Real-Time  

E-Print Network (OSTI)

to desiccation.2 Many hypotheses have been proposed to explain the high- efficiency repair, including a ringlike condensed chromosome structure that could restrict fragment DNA diffusion,3-5 a high Mn2+ concentration: With the aid of an efficient, precise, and almost error-free DNA repair system, Deinococcus radiodurans can

Cox, Michael M.

51

Monosporascus root rot/vine decline: a study of double-stranded (DS) RNA and its role in the pathogenesis of Monosporascus cannonballus on muskmelon  

E-Print Network (OSTI)

Monosporascus Root Rot/Vine Decline: A Study of Double-Stranded (ds) RNA and Its Role in the Pathogenesis of Monosporascus cannonballus on Muskmelon. (May 1997) Jeffrey Samuel Batten, B. A. , Trinity University Co-Chairs of Advisory Committee: Dr. Raymond D... hybridization between cDNA clones (pJB14 and pJB38) and dsRNA from M 96+ cannonballus isolate, Ca91-17 55 IV-2 Agarose gel and corresponding northern blot showing hybridization between cDNA clones (pJB27, pJB28, pJB39, and pJB40) and dsRNA from M...

Batten, Jeffrey Samuel

2012-06-07T23:59:59.000Z

52

Effect of silver nanoparticle and glycyrrhizic acid (SN-GLY) complex on repair of whole body radiation-induced cellular DNA damage and genomic instability in mice  

Science Journals Connector (OSTI)

Silver nanoparticles (SN) were redispersed in aqueous solution of Pluronic F127 and complexed with the phytoceutical, glycyrrhizic acid (GLY) to obtain SN-GLY complex. The ability of the SN-GLY complex to offer protection against ionising radiation in post-irradiation scenarios was evaluated in ex vivo and in vivo models using Swiss albino mice. Treatment of mouse blood leucocytes with SN-GLY immediately after 4 Gy gamma radiation exposure, ex vivo, enhanced the rate of repair of cellular DNA damages as revealed by comet assay. Exposure of mice to 4 Gy whole body gamma radiation induced formation of strand breaks in cellular DNA and the unrepaired double strand breaks eventually caused the formation of micronuclei. The post-irradiation administration of SN-GLY resulted in a faster decrease in the comet parameters indicating enhanced cellular DNA repair process and reduction in micronucleus formation. Thus the studies showed effective radiation protection by SN-GLY in post-irradiation conditions.

Dhanya K. Chandrasekharan; Cherupally Krishnan Krishnan Nair

2010-01-01T23:59:59.000Z

53

WHERE MULTIFUNCTIONAL DNA REPAIR PROTEINS MEET: MAPPING THE INTERACTION DOMAINS BETWEEN XPG AND WRN  

SciTech Connect

The rapid recognition and repair of DNA damage is essential for the maintenance of genomic integrity and cellular survival. Multiple complex and interconnected DNA damage responses exist within cells to preserve the human genome, and these repair pathways are carried out by a specifi c interplay of protein-protein interactions. Thus a failure in the coordination of these processes, perhaps brought about by a breakdown in any one multifunctional repair protein, can lead to genomic instability, developmental and immunological abnormalities, cancer and premature aging. This study demonstrates a novel interaction between two such repair proteins, Xeroderma pigmentosum group G protein (XPG) and Werner syndrome helicase (WRN), that are both highly pleiotropic and associated with inherited genetic disorders when mutated. XPG is a structure-specifi c endonuclease required for the repair of UV-damaged DNA by nucleotide excision repair (NER), and mutations in XPG result in the diseases Xeroderma pigmentosum (XP) and Cockayne syndrome (CS). A loss of XPG incision activity results in XP, whereas a loss of non-enzymatic function(s) of XPG causes CS. WRN is a multifunctional protein involved in double-strand break repair (DSBR), and consists of 3’–5’ DNA-dependent helicase, 3’–5’ exonuclease, and single-strand DNA annealing activities. Nonfunctional WRN protein leads to Werner syndrome, a premature aging disorder with increased cancer incidence. Far Western analysis was used to map the interacting domains between XPG and WRN by denaturing gel electrophoresis, which separated purifi ed full length and recombinant XPG and WRN deletion constructs, based primarily upon the length of each polypeptide. Specifi c interacting domains were visualized when probed with the secondary protein of interest which was then detected by traditional Western analysis using the antibody of the secondary protein. The interaction between XPG and WRN was mapped to the C-terminal region of XPG as well as the C-terminal region of WRN. The physical interaction between XPG and WRN links NER, (made evident by the disease XP) with DSBR, which imparts additional knowledge of the overlapping nature of these two proteins and the previously distinct DNA repair pathways they are associated with. Since genomic integrity is constantly threatened by both endogenous and exogenous (internal and external) damage, understanding the roles of these proteins in coordinating DNA repair processes with replication will signifi cantly further understanding how defects instigate physiological consequences in response to various DNA damaging sources. This ultimately contributes to our understanding of cancer and premature aging.

Rangaraj, K.; Cooper, P.K.; Trego, K.S.

2009-01-01T23:59:59.000Z

54

RADIATION SENSITIVITY & PROCESSING OF DNA DAMAGE FOLLOWING LOW DOSES OF GAMMA-RAY ALPHA PARTICLES & HZE IRRADIATION OF NORMAL DSB REPAIR DEFICIENT CELLS  

SciTech Connect

Non-homologous end joining (NHEJ) predominates in the repair of DNA double strand breaks (DSB) over homologous recombination (HR). NHEJ occurs throughout the cell cycle whereas HR occurs in late S/G2 due to the requirement of a sister chromatid (Rothkamm et al, Mol Cell Biol 23 5706-15 [2003]). To date evidence obtained with DSB repair deficient cells using pulsed-field gel electrophoresis has revealed the major pathway throughout all phases of the cell cycle for processing high dose induced DSBs is NHEJ (Wang et al, Oncogene 20 2212-24 (2001); Pluth et al, Cancer Res. 61 2649-55 [2001]). These findings however were obtained at high doses when on average >> 20-30 DSBs are formed per cell. The contribution of the repair pathways (NHEJ and HR) induced in response to DNA damage during the various phases of the cell cycle may depend upon the dose (the level of initial DSBs) especially since low levels of DSBs are induced at low dose. To date, low dose studies using NHEJ and HR deficient mutants have not been carried out to address this important question with radiations of different quality. The work presented here leads us to suggest that HR plays a relatively minor role in the repair of radiation-induced prompt DSBs. SSBs lead to the induction of DSBs which are associated specifically with S-phase cells consistent with the idea that they are formed at stalled replication forks in which HR plays a major role in repair. That DNA-PKcs is in some way involved in the repair of the precursors to replication-induced DSB remains an open question. Persistent non-DSB oxidative damage also leads to an increase in RAD51 positive DSBs. Both simple and complex non-DSB DNA damage may therefore contribute to indirect DSBs induced by ionising radiation at replication forks.

O'Neil, Peter

2009-05-15T23:59:59.000Z

55

Fanconi Anemia FANCG Protein in Mitigating Radiation- and Enzyme-Induced DNA Double-Strand Breaks by Homologous Recombination in Vertebrate Cells  

Science Journals Connector (OSTI)

...Protein in Mitigating Radiation- and Enzyme-Induced...Okayama 701-0192, Japan. Phone: 81-86-462-1111...exhibit increased MMC and radiation-induced chromosome...Okayama 701-0192, Japan. | Journal Article...pharmacology DNA drug effects radiation effects DNA Damage DNA...

Kazuhiko Yamamoto; Masamichi Ishiai; Nobuko Matsushita; Hiroshi Arakawa; Jane E. Lamerdin; Jean-Marie Buerstedde; Mitsune Tanimoto; Mine Harada; Larry H. Thompson; Minoru Takata

2003-08-01T23:59:59.000Z

56

Gene Conversion Is Strongly Induced in Human Cells by Double-strand Breaks and Is Modulated by the Expression of BCL-xL  

Science Journals Connector (OSTI)

...with the time of incubation. Factors responsible for the Pasteur...glycolytic and the respiratory energy. This appears to be a unique...date can use only respiratory energy for Pi transport. Rate-limiting factors in glycolysis and transport...

Claudia Wiese; Andrew J. Pierce; Stacey S. Gauny; Maria Jasin; and Amy Kronenberg

2002-03-01T23:59:59.000Z

57

Replication protein A and g-H2AX foci assembly is triggered by cellular response to DNA double-strand breaks  

E-Print Network (OSTI)

pathway that removes the oxidized base lesions from the genomic DNA [6]. Evidence for the stimulation

58

The impact of age, exposure and genetics on homologous recombination at the engineered repeat sequence in mice  

E-Print Network (OSTI)

Mitotic homologous recombination is a critical pathway for the repair of DNA double-strand breaks and broken replication forks. Although homologous recombination is generally error-free, recombination between misaligned ...

Wiktor-Brown, Dominika M

2007-01-01T23:59:59.000Z

59

HIV-1 Tat depresses DNA-PK{sub CS} expression and DNA repair, and sensitizes cells to ionizing radiation  

SciTech Connect

Purpose There is accumulating evidence that cancer patients with human immmunodeficiency virus-1/acquired immunodeficency syndrome (HIV-1/AIDS) have more severe tissue reactions and often develop cutaneous toxic effects when subjected to radiotherapy. Here we explored the effects of the HIV-1 Tat protein on cellular responses to ionizing radiation. Methods and Materials Two Tat-expressing cell lines, TT2 and TE671-Tat, were derived from human rhabdomyosarcoma cells by transfecting with the HIV-1 tat gene. Radiosensitivity was determined using colony-forming ability. Gene expression was assessed by cDNA microarray and immunohybridization. The Comet assay and {gamma}-H2AX foci were use to detect DNA double-strand breaks (DSBs) and repair. Radiation-induced cell cycle changes were detected by flow cytometry. Results The radiosensitivity of TT2 and TE671-Tat cells was significantly increased as compared with parental TE671 cells or the control TE671-pCI cells. Tat also increased proliferation activity. The comet assay and {gamma}H2AX foci detection revealed a decreased capacity to repair radiation-induced DNA DSBs in Tat-expressing cells. Microarray assay demonstrated that the DNA repair gene DNA-PKcs, and cell cycle-related genes Cdc20, Cdc25C, KIF2C and CTS1 were downregulated in Tat-expressing cells. Depression of DNA-PKcs in Tat-expressing cells was further confirmed by RT-PCR and immuno-hybridization analysis. Tat-expressing cells exhibited a prolonged S phase arrest after 4 Gy {gamma}-irradiation, and a noticeable delay in the initiation and elimination of radiation-induced G{sub 2}/M arrest as compared with parental cells. In addition, the G{sub 2}/M arrest was incomplete in TT2 cells. Moreover, HIV-1 Tat resulted in a constitutive overexpression of cyclin B1 protein. Conclusion HIV-1 Tat protein sensitizes cells to ionizing radiation via depressing DNA repair and dysregulating cell cycle checkpoints. These observations provide new insight into the increased tissue reactions of AIDS cancer patients to radiotherapy.

Sun Yi [Department of Radiation Toxicology and Oncology, Beijing Institute of Radiation Medicine, Beijing (China); Huang Yuechen [Department of Radiation Toxicology and Oncology, Beijing Institute of Radiation Medicine, Beijing (China); Xu Qinzhi [Department of Radiation Toxicology and Oncology, Beijing Institute of Radiation Medicine, Beijing (China); Wang Huiping [Department of Radiation Toxicology and Oncology, Beijing Institute of Radiation Medicine, Beijing (China); Bai Bei [Department of Radiation Toxicology and Oncology, Beijing Institute of Radiation Medicine, Beijing (China); Sui Jianli [Department of Radiation Toxicology and Oncology, Beijing Institute of Radiation Medicine, Beijing (China); Zhou Pingkun [Department of Radiation Toxicology and Oncology, Beijing Institute of Radiation Medicine, Beijing (China)]. E-mail: zhoupk@nic.bmi.ac.cn

2006-07-01T23:59:59.000Z

60

DNA-PK inhibition causes a low level of H2AX phosphorylation and homologous recombination repair in Medaka (Oryzias latipes) cells  

SciTech Connect

Highlights: Black-Right-Pointing-Pointer We investigated the effect of DNA-PK inhibition on DSB repair using fish cells. Black-Right-Pointing-Pointer A radiation sensitive mutant RIC1 strain showed a low level of DNA-PK activity. Black-Right-Pointing-Pointer DNA-PK dysfunction leads defects in HR repair and DNA-PKcs autophosphorylation. Black-Right-Pointing-Pointer DNA-PK dysfunction leads a slight increase in the number of 53BP1 foci after DSBs. Black-Right-Pointing-Pointer DNA-PK dysfunction leads an alternative NHEJ that depends on 53BP1. -- Abstract: Nonhomologous end joining (NHEJ) and homologous recombination (HR) are known as DNA double-strand break (DSB) repair pathways. It has been reported that DNA-PK, a member of PI3 kinase family, promotes NHEJ and aberrant DNA-PK causes NHEJ deficiency. However, in this study, we demonstrate that a wild-type cell line treated with DNA-PK inhibitor and a mutant cell line with dysfunctional DNA-PK showed decreased HR efficiency in fish cells (Medaka, Oryzias latipes). Previously, we reported that the radiation-sensitive mutant RIC1 strain has a defect in the Histone H2AX phosphorylation after {gamma}-irradiation. Here, we showed that a DNA-PK inhibitor, NU7026, treatment resulted in significant reduction in the number of {gamma}H2AX foci after {gamma}-irradiation in wild-type cells, but had no significant effect in RIC1 cells. In addition, RIC1 cells showed significantly lower levels of DNA-PK kinase activity compared with wild-type cells. We investigated NHEJ and HR efficiency after induction of DSBs. Wild-type cells treated with NU7026 and RIC1 cells showed decreased HR efficiency. These results indicated that aberrant DNA-PK causes the reduction in the number of {gamma}H2AX foci and HR efficiency in RIC1 cells. We performed phosphorylated DNA-PKcs (Thr2609) and 53BP1 focus assay after {gamma}-irradiation. RIC1 cells showed significant reduction in the number of phosphorylated DNA-PKcs foci and no deference in the number of 53BP1 foci compared with wild-type cells. These results suggest that low level of DNA-PK activity causes aberrant DNA-PKcs autophosphorylation in RIC1 cells. It is known that 53BP1 is involved in both DNA-PK dependent and independent NHEJ. Therefore we suggest that DNA-PK independent NHEJ repair DSBs under the condition of decreased DNA-PK activity, which causes reduction of HR efficiency.

Urushihara, Yusuke [Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba 277-8562 (Japan)] [Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba 277-8562 (Japan); Kobayashi, Junya [Department of Genome Repair Dynamics, Radiation Biology Center, Kyoto University, Kyoto 606-8501 (Japan)] [Department of Genome Repair Dynamics, Radiation Biology Center, Kyoto University, Kyoto 606-8501 (Japan); Matsumoto, Yoshihisa [Research Laboratory for Nuclear Reactors, Tokyo Institute of Technology, Tokyo 152-8550 (Japan)] [Research Laboratory for Nuclear Reactors, Tokyo Institute of Technology, Tokyo 152-8550 (Japan); Komatsu, Kenshi [Department of Genome Repair Dynamics, Radiation Biology Center, Kyoto University, Kyoto 606-8501 (Japan)] [Department of Genome Repair Dynamics, Radiation Biology Center, Kyoto University, Kyoto 606-8501 (Japan); Oda, Shoji [Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba 277-8562 (Japan)] [Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba 277-8562 (Japan); Mitani, Hiroshi, E-mail: mitani@k.u-tokyo.ac.jp [Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba 277-8562 (Japan)] [Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba 277-8562 (Japan)

2012-12-14T23:59:59.000Z

Note: This page contains sample records for the topic "double-strand break repair" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


61

Radiosensitization of MDA-MB-231 breast tumor cells by adenovirus-mediated overexpression of a fragment of the XRCC4 protein  

Science Journals Connector (OSTI)

...Repair of DNA double strand breaks by non-homologous end joining. Biochimie 2003;85:1161-73. 9 El-Khamisy SF, Saifi GM, Weinfeld M, et al. Defective DNA single-strand break repair in spinocerebellar ataxia with axonal neuropathy-1...

Kara R. Jones; David A. Gewirtz; Steven M. Yannone; Shaoming Zhou; David G. Schatz; Kristoffer Valerie; Lawrence F. Povirk

2005-10-01T23:59:59.000Z

62

MDC1 Cleavage by Caspase-3: A Novel Mechanism for Inactivating the DNA Damage Response during Apoptosis  

Science Journals Connector (OSTI)

...double-strand break repair and may be a novel therapeutic...commonly used or are in the pipeline, yet virtually all pancreatic...these lines, the DNA repair pathway is an attractive...timely drug development pipeline. Therefore, the model...components of the DNA repair complex (31, 33...

Stéphanie Solier and Yves Pommier

2011-02-01T23:59:59.000Z

63

Structural biology and bioinformatics in drug design: opportunities and challenges for target identification and lead discovery  

Science Journals Connector (OSTI)

...by charged and polar patches. Figure 7 The human...essential for normal DNA repair, there is an advantage...structure determination pipeline. Proc. Natl Acad...double-strand-break-repair mutant mammalian cells...gene and its role in DNA repair and V(D)J recombination...

2006-01-01T23:59:59.000Z

64

Structure of gramicidin D-RbCl complex at atomic resolution from low-temperature synchrotron data: interactions of double-stranded gramicidin channel contents and cations with channel wall  

SciTech Connect

Gramicidin D (gD) is a naturally occurring ionophoric antibiotic that forms membrane channels specific for monovalent cations. The crystal structure of the RbCl complex of gD has been determined at 1.14 {angstrom} resolution from low-temperature (100 K) synchrotron-radiation data with a final R of 16%. The structure was refined with anisotropic temperature factors for all non-H atoms and with partial occupancies for many of them. The asymmetric unit in the crystal contains four crystallographically independent molecules that form two right-handed antiparallel double-stranded dimers. There are seven distinct rubidium-binding sites in each dimeric channel. The occupancy factors of Rb cations are between 0.11 and 0.35 and the total ion contents of the two crystallographically independent channels are 1.59 and 1.22 ions, respectively. Although each channel is 'chemically symmetrical', the side-chain conformations, the distributions of rubidium cations and their binding sites in the two independent channels are not. Cations are 'coordinated' by delocalized {pi}-electrons of three to five carbonyl groups that together with peptide backbone chains form the gramicidin channel walls. The water:cation ratio in the channel interior is four or five:one, and five or six waters separate Rb cations during their passage through the channel.

Glówka, M.L.; Olczak, A.; Bojarska, J.; Szczesio, M.; Duax, W.L.; Burkhart, B.M.; Pangborn, W.A.; Langs, D.A.; Wawrzak, Z. (Poland); (NWU); (Hauptman)

2010-03-05T23:59:59.000Z

65

Photoaffinity Labeling of Mouse Fibroblast Enzymes by a Base Excision Repair Intermediate: New Evidence on the Role of PARP-1 in DNA Repair  

SciTech Connect

To examine mammalian base excision repair (BER) enzymes interacting with DNA intermediates formed during BER, we used a novel photoaffinity labeling probe and mouse embryonic fibroblast (MEF) crude extract. The probe was formed in situ, using an end-labeled oligonucleotide containing a synthetic abasic site; this site was incised by AP endonuclease creating a nick with 3' hydroxyl and 5' reduced sugar phosphate groups at the margins, and then a dNMP carrying a photoreactive adduct was introduced at the 3' hydroxyl group. With near UV-light exposure (312nm) of the extract-probe mixture, only six proteins were strongly labeled, including poly (ADP-ribose) polymerase (PARP-1) and the well-known BER participants flap endonuclease (FEN-1), DNA polymerase b (b-pol), and AP endonuclease (APE). The amount of probe crosslinked to PARP-1 was greater than that crosslinked to the other proteins. The specificity of PARP-1 labeling was examined by competition experiments involving various oligonucleotide competitors; competition of labeling by the probe was much greater for the BER intermediates tested than for normal double-stranded DNA. The specificity of PARP-1 labeling also was examined using DNA probes with alternate structures; PARP-1 labeling was stronger with a DNA oligomer representing a BER intermediate than with a molecule representing a nick in double-stranded DNA. These results identifying interaction of PARP-1 with a BER intermediate are discussed in light of PARP-1's role in mammalian BER.

Lavrik, Olga I.(National Institute of Environmental Health Sciences) [National Institute of Environmental Health Sciences; Prasad, Rajendra (National Institute of Environmental Health Sciences) [National Institute of Environmental Health Sciences; Sobol, Robert W.(National Institute of Environmental Health Sciences) [National Institute of Environmental Health Sciences; Horton, Julie K.(National Institute of Environmental Health Sciences) [National Institute of Environmental Health Sciences; Ackerman, Eric J.(BATTELLE (PACIFIC NW LAB)) [BATTELLE (PACIFIC NW LAB); Wilson, Samuel H.(National Institute of Environmental Health Sciences) [National Institute of Environmental Health Sciences

2001-07-06T23:59:59.000Z

66

in this issue nature methods | VOL.9 NO.10 | OCTOBER 2012  

E-Print Network (OSTI)

difficult. Mirny, Dekker and colleagues describe a pipeline to normalize Hi-C data (which provides an all insertions and deletions during the repair of an endonuclease-mediated DNA double-strand break (DSB) is increasingly exploited to carry out precise gene disruption. However, the DSB can be precisely repaired and re

Cai, Long

67

The Sensitivity of MCF10A Breast Epithelial Cells to Alkylating Drugs is Enhanced by the Inhibition of O6-Methylguanine-DNA Methyltransferase Transcription with a Synthetic Double Strand DNA Oligonucleotide  

Science Journals Connector (OSTI)

Cytoxicity of alkylating chemotherapeutic drugs is affected by the cellular content of the enzyme O6-methylguanine-DNA methyl transferase (MGMT). Since high levels ... of the enzyme confer the efficient repair of...

Laura Mariani; Antonella Piccirilli; Lorenzo Citti…

2002-06-01T23:59:59.000Z

68

BioMed Central Page 1 of 10  

E-Print Network (OSTI)

of neocarzinostatin-induced DNA double-strand breaks. Background Ionizing radiation induces multiple lesions in cell) in proportion to the radiation dose. Among the enzymes that have evolved for the repair of radiation damage-B activation and proper management of the oxidative stress inherent in radiation exposure. DNA

Boyer, Edmond

69

Automated analysis of time-lapse fluorescence microscopy images: from live cell images to intracellular foci  

Science Journals Connector (OSTI)

......varying sizes and shapes. The segmentation pipeline consists of three steps: (i) detection...image stack. In the first step of the pipeline, a marker is identified for each potential...related to the DNA double-strand break repair kinetics. For example, we found in normal......

Oleh Dzyubachyk; Jeroen Essers; Wiggert A. van Cappellen; Céline Baldeyron; Akiko Inagaki; Wiro J. Niessen; Erik Meijering

2010-10-01T23:59:59.000Z

70

DNA Damage and Repair in Translational Oncology: An Overview  

Science Journals Connector (OSTI)

...is a molecular sensor of DNA breaks that facilitates DNA repair and controls genomic stability and apoptosis. PARP inhibitors...PARP-1 inhibitor NSC 737664 entered the NCI clinical development pipeline, we initiated a program to model pharmacodynamic responses...

Eddie Reed

2010-09-15T23:59:59.000Z

71

“Contextual” Synthetic Lethality and/or Loss of Heterozygosity: Tumor Hypoxia and Modification of DNA Repair  

Science Journals Connector (OSTI)

...Break DNA Damage Signaling and Repair Dorine Rossetto 1 Andrew W...machineries for chromosomal DNA repair. Genes Dev 2004;18:602-16...Plummer R . Perspective on the pipeline of drugs being developed with...hypoxia and modification of DNA repair. Clin Cancer Res 2010;16...

Norman Chan and Robert G. Bristow

2010-09-15T23:59:59.000Z

72

INTERNAL REPAIR OF PIPELINES  

SciTech Connect

The two broad categories of deposited weld metal repair and fiber-reinforced composite repair technologies were reviewed for potential application for internal repair of gas transmission pipelines. Both are used to some extent for other applications and could be further developed for internal, local, structural repair of gas transmission pipelines. Preliminary test programs were developed for both deposited weld metal repairs and for fiber-reinforced composite repair. To date, all of the experimental work pertaining to the evaluation of potential repair methods has focused on fiber-reinforced composite repairs. Hydrostatic testing was also conducted on four pipeline sections with simulated corrosion damage: two with composite liners and two without.

Robin Gordon; Bill Bruce; Nancy Porter; Mike Sullivan; Chris Neary

2003-05-01T23:59:59.000Z

73

Rapid road repair vehicle  

DOE Patents (OSTI)

Disclosed is a rapid road repair vehicle capable of moving over a surface to be repaired at near normal posted traffic speeds to scan for and find at the high rate of speed, imperfections in the pavement surface, prepare the surface imperfection for repair by air pressure and vacuum cleaning, applying a correct amount of the correct patching material to effect the repair, smooth the resulting repaired surface, and catalog the location and quality of the repairs for maintenance records of the road surface. The rapid road repair vehicle can repair surface imperfections at lower cost, improved quality, at a higher rate of speed than was not heretofor possible, with significantly reduced exposure to safety and health hazards associated with this kind of road repair activities in the past. 2 figs.

Mara, L.M.

1998-05-05T23:59:59.000Z

74

Rapid road repair vehicle  

DOE Patents (OSTI)

Disclosed is a rapid road repair vehicle capable of moving over a surface to be repaired at near normal posted traffic speeds to scan for and find an the high rate of speed, imperfections in the pavement surface, prepare the surface imperfection for repair by air pressure and vacuum cleaning, applying a correct amount of the correct patching material to effect the repair, smooth the resulting repaired surface, and catalog the location and quality of the repairs for maintenance records of the road surface. The rapid road repair vehicle can repair surface imperfections at lower cost, improved quality, at a higher rate of speed than was was heretofor possible, with significantly reduced exposure to safety and health hazards associated with this kind of road repair activities in the past.

Mara, Leo M. (Livermore, CA)

1998-01-01T23:59:59.000Z

75

Molecular Pathways: Exploiting Tumor-Specific Molecular Defects in DNA Repair Pathways for Precision Cancer Therapy  

Science Journals Connector (OSTI)

...represent genetic aberrations that are specific to cancer cells and not present in healthy...phenotype, which allows incipient cancer cells to accumulate additional cancer-promoting...break (DSB) repair mechanisms has led to the development of targeted therapeutic...

Felix Dietlein and H. Christian Reinhardt

2014-12-01T23:59:59.000Z

76

The DNA Damage Response Signaling Cascade Regulates Proliferation of the Phytopathogenic Fungus Ustilago maydis in Planta  

Science Journals Connector (OSTI)

...and Sanchez, 2004) in a broad range of eukaryotes, including U. maydis...double-strand breaks in DNA; and ionizing radiation (IR), which generates double-strand...were irradiated at the indicated dose after being spotted. The spots...

Carmen de Sena-Tomás; Alfonso Fernández-Álvarez; William K. Holloman; José Pérez-Martín

2011-04-08T23:59:59.000Z

77

Template Role of Double-Stranded RNA in Tombusvirus Replication  

Science Journals Connector (OSTI)

...repRNA). Briefly, the method for the CFE assay is as follows: in vitro TBSV replication assays were performed by using 2 mul of CFE, 0.25 mug DI-72 (+)repRNA T7-made...32P]UTP in a 20-mul total volume. The CFE assay was performed at 25C for 3 h and stopped...

Nikolay Kovalev; Judit Pogany; Peter D. Nagy

2014-03-05T23:59:59.000Z

78

Chemically accurate coarse graining of double-stranded DNA  

Science Journals Connector (OSTI)

...Phys Chem B 112, 9135 ZZQQhy9145. 8. Chen, A. A & Pappu, R. V. (2007) Parameters of monovalent ions in the amber-99...Chem B 111, 11884 ZZQQhy11887. 14 9. Chen, A. A & Pappu, R. V. (2007) Quantitative characterization of ion pairing...

Alexey Savelyev; Garegin A. Papoian

2010-01-01T23:59:59.000Z

79

Recognition of Double-Stranded RNA by Proteins  

E-Print Network (OSTI)

. Stephens Peter A. Beal University of Utah, Department of Chemistry, 315 South 1400 East, Room 2020, Salt level. In contrast, Correspondence to: Peter A. Beal; email: beal@chemistry. utah.edu). Contract grant

Beal, Peter A.

80

Nanowire Micronetworks from Carbon-Black Nanoparticles | Advanced Photon  

NLE Websites -- All DOE Office Websites (Extended Search)

A Key Step in Repairing DNA Double-Strand Breaks A Key Step in Repairing DNA Double-Strand Breaks An X-ray Rainbow An Insulating Breakthrough Science Highlights Archives: 2013 | 2012 | 2011 | 2010 2009 | 2008 | 2007 | 2006 2005 | 2004 | 2003 | 2002 2001 | 2000 | 1998 | Subscribe to APS Science Highlights rss feed Nanowire Micronetworks from Carbon-Black Nanoparticles JANUARY 11, 2007 Bookmark and Share Fig. 1. Diagram showing CB segregation to triple junctions during press sintering. Inset: USAXS image acquired from a press-sintered CB/PMMA sample with 1% CB mass fraction. Self-assembly is likely to play a decisive role in producing nanoscale elements for three dimensional (3D) electronic and nanoscale electro-mechanical systems. Until now, however, the difficult problem of fabricating a complex, interconnected 3D system of conductive nanoscale

Note: This page contains sample records for the topic "double-strand break repair" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


81

INTERNAL REPAIR OF PIPELINES  

SciTech Connect

The two broad categories of fiber-reinforced composite liner repair and deposited weld metal repair technologies were reviewed and evaluated for potential application for internal repair of gas transmission pipelines. Both are used to some extent for other applications and could be further developed for internal, local, structural repair of gas transmission pipelines. Principal conclusions from a survey of natural gas transmission industry pipeline operators can be summarized in terms of the following performance requirements for internal repair: (1) Use of internal repair is most attractive for river crossings, under other bodies of water, in difficult soil conditions, under highways, under congested intersections, and under railway crossings. (2) Internal pipe repair offers a strong potential advantage to the high cost of horizontal direct drilling when a new bore must be created to solve a leak or other problem. (3) Typical travel distances can be divided into three distinct groups: up to 305 m (1,000 ft.); between 305 m and 610 m (1,000 ft. and 2,000 ft.); and beyond 914 m (3,000 ft.). All three groups require pig-based systems. A despooled umbilical system would suffice for the first two groups which represents 81% of survey respondents. The third group would require an onboard self-contained power unit for propulsion and welding/liner repair energy needs. (4) The most common size range for 80% to 90% of operators surveyed is 508 mm (20 in.) to 762 mm (30 in.), with 95% using 558.8 mm (22 in.) pipe. Evaluation trials were conducted on pipe sections with simulated corrosion damage repaired with glass fiber-reinforced composite liners, carbon fiber-reinforced composite liners, and weld deposition. Additional un-repaired pipe sections were evaluated in the virgin condition and with simulated damage. Hydrostatic failure pressures for pipe sections repaired with glass fiber-reinforced composite liner were only marginally greater than that of pipe sections without liners, indicating that this type of liner is only marginally effective at restoring the pressure containing capabilities of pipelines. Failure pressures for larger diameter pipe repaired with a semi-circular patch of carbon fiber-reinforced composite lines were also marginally greater than that of a pipe section with un-repaired simulated damage without a liner. These results indicate that fiber reinforced composite liners have the potential to increase the burst pressure of pipe sections with external damage Carbon fiber based liners are viewed as more promising than glass fiber based liners because of the potential for more closely matching the mechanical properties of steel. Pipe repaired with weld deposition failed at pressures lower than that of un-repaired pipe in both the virgin and damaged conditions, indicating that this repair technology is less effective at restoring the pressure containing capability of pipe than a carbon fiber-reinforced liner repair. Physical testing indicates that carbon fiber-reinforced liner repair is the most promising technology evaluated to-date. In lieu of a field installation on an abandoned pipeline, a preliminary nondestructive testing protocol is being developed to determine the success or failure of the fiber-reinforced liner pipeline repairs. Optimization and validation activities for carbon-fiber repair methods are ongoing.

Bill Bruce; Nancy Porter; George Ritter; Matt Boring; Mark Lozev; Ian Harris; Bill Mohr; Dennis Harwig; Robin Gordon; Chris Neary; Mike Sullivan

2005-07-20T23:59:59.000Z

82

Local collapse of gas pipelines under sleeve repairs  

Science Journals Connector (OSTI)

Local collapse of the pipe wall under full encirclement sleeve reinforcements is associated with breaks and blow outs that cause large gas losses and abrupt depressurisation in gas pipelines. Although these defects do not represent an imminent risk of failure, they should be eliminated because they impede the normal passage of the “instrumented pig” for internal inspection. Four failed repairs were experimentally evaluated, and the effects of different geometric factors were numerically assessed via non-linear numerical modelling of fluid flow and pipe response. All possible causes of the appearance of these defects and measures to minimise their occurrence were evaluated. The position of the repaired portion with respect to the blow out, local geometry of the repair and previous defects, and the amount of gas caught in the interstice between the pipe and the reinforcement, have an important part in the event. The measures for the prevention of this problem involve the use of fillers and improved construction of repair sleeves.

J.L Otegui; S Urquiza; A Rivas; A Trunzo

2000-01-01T23:59:59.000Z

83

INTERNAL REPAIR OF PIPELINES  

SciTech Connect

The two broad categories of fiber-reinforced composite liner repair and deposited weld metal repair technologies were reviewed and evaluated for potential application for internal repair of gas transmission pipelines. Both are used to some extent for other applications and could be further developed for internal, local, structural repair of gas transmission pipelines. Principal conclusions from a survey of natural gas transmission industry pipeline operators can be summarized in terms of the following performance requirements for internal repair: (1) Use of internal repair is most attractive for river crossings, under other bodies of water, in difficult soil conditions, under highways, under congested intersections, and under railway crossings. (2) Internal pipe repair offers a strong potential advantage to the high cost of horizontal direct drilling when a new bore must be created to solve a leak or other problem. (3) Typical travel distances can be divided into three distinct groups: up to 305 m (1,000 ft.); between 305 m and 610 m (1,000 ft. and 2,000 ft.); and beyond 914 m (3,000 ft.). All three groups require pig-based systems. A despooled umbilical system would suffice for the first two groups which represents 81% of survey respondents. The third group would require an onboard self-contained power unit for propulsion and welding/liner repair energy needs. (4) The most common size range for 80% to 90% of operators surveyed is 508 mm (20 in.) to 762 mm (30 in.), with 95% using 558.8 mm (22 in.) pipe. Evaluation trials were conducted on pipe sections with simulated corrosion damage repaired with glass fiber-reinforced composite liners, carbon fiber-reinforced composite liners, and weld deposition. Additional un-repaired pipe sections were evaluated in the virgin condition and with simulated damage. Hydrostatic failure pressures for pipe sections repaired with glass fiber-reinforced composite liner were only marginally greater than that of pipe sections without liners, indicating that this type of liner is only marginally effective at restoring the pressure containing capabilities of pipelines. Failure pressures for larger diameter pipe repaired with a semi-circular patch of carbon fiber-reinforced composite lines were also marginally greater than that of a pipe section with un-repaired simulated damage without a liner. These results indicate that fiber reinforced composite liners have the potential to increase the burst pressure of pipe sections with external damage Carbon fiber based liners are viewed as more promising than glass fiber based liners because of the potential for more closely matching the mechanical properties of steel. Pipe repaired with weld deposition failed at pressures lower than that of un-repaired pipe in both the virgin and damaged conditions, indicating that this repair technology is less effective at restoring the pressure containing capability of pipe than a carbon fiber-reinforced liner repair. Physical testing indicates that carbon fiber-reinforced liner repair is the most promising technology evaluated to-date. The first round of optimization and validation activities for carbon-fiber repairs are complete. Development of a comprehensive test plan for this process is recommended for use in the field trial portion of this program.

Robin Gordon; Bill Bruce; Ian Harris; Dennis Harwig; George Ritter; Bill Mohr; Matt Boring; Nancy Porter; Mike Sullivan; Chris Neary

2004-12-31T23:59:59.000Z

84

INTERNAL REPAIR OF PIPELINES  

SciTech Connect

The two broad categories of fiber-reinforced composite liner repair and deposited weld metal repair technologies were reviewed and evaluated for potential application for internal repair of gas transmission pipelines. Both are used to some extent for other applications and could be further developed for internal, local, structural repair of gas transmission pipelines. Principal conclusions from a survey of natural gas transmission industry pipeline operators can be summarized in terms of the following performance requirements for internal repair: (1) Use of internal repair is most attractive for river crossings, under other bodies of water, in difficult soil conditions, under highways, under congested intersections, and under railway. (2) Internal pipe repair offers a strong potential advantage to the high cost of horizontal direct drilling when a new bore must be created to solve a leak or other problem. (3) Typical travel distances can be divided into three distinct groups: up to 305 m (1,000 ft.); between 305 m and 610 m (1,000 ft. and 2,000 ft.); and beyond 914 m (3,000 ft.). All three groups require pig-based systems. A despooled umbilical system would suffice for the first two groups which represents 81% of survey respondents. The third group would require an onboard self-contained power unit for propulsion and welding/liner repair energy needs. (4) The most common size range for 80% to 90% of operators surveyed is 508 mm (20 in.) to 762 mm (30 in.), with 95% using 558.8 mm (22 in.) pipe. Evaluation trials were conducted on pipe sections with simulated corrosion damage repaired with glass fiber-reinforced composite liners, carbon fiber-reinforced composite liners, and weld deposition. Additional un-repaired pipe sections were evaluated in the virgin condition and with simulated damage. Hydrostatic failure pressures for pipe sections repaired with glass fiber-reinforced composite liner were only marginally greater than that of pipe sections without liners, indicating that this type of liner is only marginally effective at restoring the pressure containing capabilities of pipelines. Failure pressures for larger diameter pipe repaired with a semi-circular patch of carbon fiber-reinforced composite lines were also marginally greater than that of a pipe section with un-repaired simulated damage without a liner. These results indicate that fiber reinforced composite liners have the potential to increase the burst pressure of pipe sections with external damage Carbon fiber based liners are viewed as more promising than glass fiber based liners because of the potential for more closely matching the mechanical properties of steel. Pipe repaired with weld deposition failed at pressures lower than that of un-repaired pipe in both the virgin and damaged conditions, indicating that this repair technology is less effective at restoring the pressure containing capability of pipe than a carbon fiber-reinforced liner repair. Physical testing indicates that carbon fiber-reinforced liner repair is the most promising technology evaluated to-date. Development of a comprehensive test plan for this process is recommended for use in the field trial portion of this program.

Robin Gordon; Bill Bruce; Ian Harris; Dennis Harwig; George Ritter; Bill Mohr; Matt Boring; Nancy Porter; Mike Sullivan; Chris Neary

2004-08-17T23:59:59.000Z

85

Breaking Earth Poems  

E-Print Network (OSTI)

CALIFORNIA RIVERSIDE Breaking Earth Poems A Thesis submittedFestival……………………………………………………………………..…..14 Earth Against Mylittle else in their hands. Earth Against My Back I lay in

Hernandez, Scott Mcnaul

2012-01-01T23:59:59.000Z

86

Rapid road repair vehicle  

DOE Patents (OSTI)

Disclosed are improvments to a rapid road repair vehicle comprising an improved cleaning device arrangement, two dispensing arrays for filling defects more rapidly and efficiently, an array of pre-heaters to heat the road way surface in order to help the repair material better bond to the repaired surface, a means for detecting, measuring, and computing the number, location and volume of each of the detected surface imperfection, and a computer means schema for controlling the operation of the plurality of vehicle subsystems. The improved vehicle is, therefore, better able to perform its intended function of filling surface imperfections while moving over those surfaces at near normal traffic speeds.

Mara, Leo M. (Livermore, CA)

1999-01-01T23:59:59.000Z

87

RAP80 Acts Independently of BRCA1 in Repair of Topoisomerase II Poison-Induced DNA Damage  

Science Journals Connector (OSTI)

...Implications for the repair of topoisomerase I-mediated DNA damage. J Biol Chem 2004;279:37343-8. 15 El-Khamisy SF , Saifi GM, Weinfeld M, et al. Defective DNA single-strand break repair in spinocerebellar ataxia with axonal neuropathy-1...

Junko Iijima; Zhihong Zeng; Shunichi Takeda; Yoshihito Taniguchi

2010-11-01T23:59:59.000Z

88

INTERNAL REPAIR OF PIPELINES  

SciTech Connect

The two broad categories of deposited weld metal repair and fiber-reinforced composite liner repair technologies were reviewed for potential application for internal repair of gas transmission pipelines. Both are used to some extent for other applications and could be further developed for internal, local, structural repair of gas transmission pipelines. Preliminary test programs were developed for both deposited weld metal repair and for fiber-reinforced composite liner repair. Evaluation trials have been conducted using a modified fiber-reinforced composite liner provided by RolaTube and pipe sections without liners. All pipe section specimens failed in areas of simulated damage. Pipe sections containing fiber-reinforced composite liners failed at pressures marginally greater than the pipe sections without liners. The next step is to evaluate a liner material with a modulus of elasticity approximately 95% of the modulus of elasticity for steel. Preliminary welding parameters were developed for deposited weld metal repair in preparation of the receipt of Pacific Gas & Electric's internal pipeline welding repair system (that was designed specifically for 559 mm (22 in.) diameter pipe) and the receipt of 559 mm (22 in.) pipe sections from Panhandle Eastern. The next steps are to transfer welding parameters to the PG&E system and to pressure test repaired pipe sections to failure. A survey of pipeline operators was conducted to better understand the needs and performance requirements of the natural gas transmission industry regarding internal repair. Completed surveys contained the following principal conclusions: (1) Use of internal weld repair is most attractive for river crossings, under other bodies of water, in difficult soil conditions, under highways, under congested intersections, and under railway crossings. (2) Internal pipe repair offers a strong potential advantage to the high cost of horizontal direct drilling (HDD) when a new bore must be created to solve a leak or other problem. (3) Typical travel distances can be divided into three distinct groups: up to 305 m (1,000 ft.); between 305 m and 610 m (1,000 ft. and 2,000 ft.); and beyond 914 m (3,000 ft.). All three groups require pig-based systems. A despooled umbilical system would suffice for the first two groups which represents 81% of survey respondents. The third group would require an onboard self-contained power unit for propulsion and welding/liner repair energy needs. (4) Pipe diameter sizes range from 50.8 mm (2 in.) through 1,219.2 mm (48 in.). The most common size range for 80% to 90% of operators surveyed is 508 mm to 762 mm (20 in. to 30 in.), with 95% using 558.8 mm (22 in.) pipe. An evaluation of potential repair methods clearly indicates that the project should continue to focus on the development of a repair process involving the use of GMAW welding and on the development of a repair process involving the use of fiber-reinforced composite liners.

Robin Gordon; Bill Bruce; Ian Harris; Dennis Harwig; Nancy Porter; Mike Sullivan; Chris Neary

2004-04-12T23:59:59.000Z

89

Electric motor model repair specifications  

SciTech Connect

These model repair specifications list the minimum requirements for repair and overhaul of polyphase AC squireel cage induction motors. All power ranges, voltages, and speeds of squirrel cage motors are covered.

NONE

1995-08-01T23:59:59.000Z

90

Heat induces gene amplification in cancer cells  

SciTech Connect

Highlights: Black-Right-Pointing-Pointer This study discovered that heat exposure (hyperthermia) results in gene amplification in cancer cells. Black-Right-Pointing-Pointer Hyperthermia induces DNA double strand breaks. Black-Right-Pointing-Pointer DNA double strand breaks are considered to be required for the initiation of gene amplification. Black-Right-Pointing-Pointer The underlying mechanism of heat-induced gene amplification is generation of DNA double strand breaks. -- Abstract: Background: Hyperthermia plays an important role in cancer therapy. However, as with radiation, it can cause DNA damage and therefore genetic instability. We studied whether hyperthermia can induce gene amplification in cancer cells and explored potential underlying molecular mechanisms. Materials and methods: (1) Hyperthermia: HCT116 colon cancer cells received water-submerged heating treatment at 42 or 44 Degree-Sign C for 30 min; (2) gene amplification assay using N-(phosphoacetyl)-L-aspartate (PALA) selection of cabamyl-P-synthetase, aspartate transcarbarmylase, dihydro-orotase (cad) gene amplified cells; (3) southern blotting for confirmation of increased cad gene copies in PALA-resistant cells; (4) {gamma}H2AX immunostaining to detect {gamma}H2AX foci as an indication for DNA double strand breaks. Results: (1) Heat exposure at 42 or 44 Degree-Sign C for 30 min induces gene amplification. The frequency of cad gene amplification increased by 2.8 and 6.5 folds respectively; (2) heat exposure at both 42 and 44 Degree-Sign C for 30 min induces DNA double strand breaks in HCT116 cells as shown by {gamma}H2AX immunostaining. Conclusion: This study shows that heat exposure can induce gene amplification in cancer cells, likely through the generation of DNA double strand breaks, which are believed to be required for the initiation of gene amplification. This process may be promoted by heat when cellular proteins that are responsible for checkpoints, DNA replication, DNA repair and telomere functions are denatured. To our knowledge, this is the first study to provide direct evidence of hyperthermia induced gene amplification.

Yan, Bin, E-mail: yanbin@mercyhealth.com [Department of Radiation Oncology, University of Mississippi Medical Center, Jackson, MS 39213 (United States) [Department of Radiation Oncology, University of Mississippi Medical Center, Jackson, MS 39213 (United States); Mercy Cancer Center, Mercy Medical Center-North Iowa, Mason City, IA 50401 (United States); Ouyang, Ruoyun [Department of Respiratory Medicine, The Second Xiangya Hospital, Xinagya School of Medicine, Central South University, Changsha 410011 (China)] [Department of Respiratory Medicine, The Second Xiangya Hospital, Xinagya School of Medicine, Central South University, Changsha 410011 (China); Huang, Chenghui [Department of Radiation Oncology, University of Mississippi Medical Center, Jackson, MS 39213 (United States) [Department of Radiation Oncology, University of Mississippi Medical Center, Jackson, MS 39213 (United States); Department of Oncology, The Third Xiangya Hospital, Xinagya School of Medicine, Central South University, Changsha 410013 (China); Liu, Franklin [Department of Radiation Oncology, Duke University Medical Center, Durham, NC 27710 (United States)] [Department of Radiation Oncology, Duke University Medical Center, Durham, NC 27710 (United States); Neill, Daniel [Department of Radiation Oncology, University of Mississippi Medical Center, Jackson, MS 39213 (United States)] [Department of Radiation Oncology, University of Mississippi Medical Center, Jackson, MS 39213 (United States); Li, Chuanyuan [Dermatology, Duke University Medical Center, Durham, NC 27710 (United States)] [Dermatology, Duke University Medical Center, Durham, NC 27710 (United States); Dewhirst, Mark [Department of Radiation Oncology, Duke University Medical Center, Durham, NC 27710 (United States)] [Department of Radiation Oncology, Duke University Medical Center, Durham, NC 27710 (United States)

2012-10-26T23:59:59.000Z

91

Modulated Tool-Path (MTP) Chip Breaking System  

SciTech Connect

The Modulated Tool-Path (MTP) Chip Breaking System produces user-selectable chip lengths and workpiece finishes and is compatible with any material, workpiece shape, and depth of cut. The MTP chip breaking system consistently creates the desired size of chips regardless of workpiece size, shape, or material, and the machine operator does not need to make any adjustments during the machining operation. The system's programmer configures the part program that commands the machine tool to move in a specific fashion to deliver the desired part size, shape, chip length, and workpiece surface finish. The MTP chip breaking system helps manufacturers avoid the detrimental effects of continuous chips, including expensive repair costs, delivery delays, and hazards to personnel.

Graham, K. B.

2010-04-01T23:59:59.000Z

92

Will the Web break?  

Science Journals Connector (OSTI)

...Discussion 1003 51 Discussion Meeting Issue Web science: a new frontier organized and edited...Wendy Hall and James A. Hendler Will the Web break? Jonathan Zittrain * e-mail: zittrain...contribution of 15 to a Discussion Meeting Issue Web science: a new frontier . What is the Web...

2013-01-01T23:59:59.000Z

93

SuperBreak: Using Interactivity to Enhance Ergonomic Typing Breaks  

E-Print Network (OSTI)

SuperBreak: Using Interactivity to Enhance Ergonomic Typing Breaks Dan Morris, A.J. Bernheim Brush Repetitive strain injuries and ergonomics concerns have become increasingly significant health issues software mechanisms exist for managing ergonomics; the most well-known are "break-reminder" packages

Rajamani, Sriram K.

94

$R$-Parity Breaking Phenomenology  

E-Print Network (OSTI)

We review various features of the $R$-parity breaking phenomenology, with particular attention to the low energy observables, and to the patterns of the $R$-parity breaking interactions that arise in Grand Unified models.

Francesco Vissani

1996-03-11T23:59:59.000Z

95

Application of a Downhole Flowmeter to Detecting Casing Breaks in a Geothermal Well  

SciTech Connect

The downhole flowmeter logging system for high temperature geothermal wells developed at Wairakei, New Zealand, is proving to be an invaluable tool for use during workovers to repair or reinstate problem wells. This contribution describes a straightforward example of identifying breaks in plain casing near the top of a productive well.

Syms, Margot C.; Syms, Peter H.; Bixley, Paul F.

1980-12-16T23:59:59.000Z

96

Models of Dynamical Supersymmetry Breaking  

E-Print Network (OSTI)

We review a class of models of dynamical supersymmetry breaking, and give a unified description of these models.

Lisa Randall

1997-06-23T23:59:59.000Z

97

Breaking Down the Barriers  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Breaking Down the Barriers Breaking Down the Barriers Engaging Agency Legal Resources to be Part of the Solution Daniel Gore US Coast Guard Energy Manager X ESPC ISC Kodiak ESPC/UESC Unit Contract Type Coast Guard Alternatively Financed Project Status Estimated Contract Value (Millions) Under Consideration Initial Proposal Delayed by lack of Contracting Officer or Champion Detailed Design Study Recently Awarded TRACEN Cape May ESPC X TRACEN Petaluma PPA X X ISC San Pedro UESC X X CG Academy ESPC X TRACEN Cape May UESC X Air Station Borenquin ESPC X X Sector New York (3 sites) ESPC X X CG Yard (BAMF) ESPC X E-City ESPC X West Coast - 9 Sites ESPC X Five Essentials for Alt. Financed Project * Site approval * Technical Champion * Contracting Officer * Financial Analyst * Legal Support

98

Energy Loss by Breaking waves  

Science Journals Connector (OSTI)

Observations of the frequency of wind wave breaking in deep water are combined with laboratory estimates of the rate of energy loss a from single breaking wave to infer the net rate of energy transfer to the mixed layer from breaking waves, as a ...

S. A. Thorpe

1993-11-01T23:59:59.000Z

99

Inspect and Repair Steam Traps  

Energy.gov (U.S. Department of Energy (DOE))

This tip sheet on inspecting and repairing steam traps provides how-to advice for improving industrial steam systems using low-cost, proven practices and technologies.

100

DNA Repair Mechanisms and Their Biological Roles in the Malaria Parasite Plasmodium falciparum  

Science Journals Connector (OSTI)

...Fabre, and M Kupiec. 2013. Effect of nuclear architecture on the efficiency of double-strand...2013. Gene copy-number alterations: a cost-benefit analysis. Cell 152 :394-405...pnas.93.3.1130 . 140. Crabb, BS , and AF Cowman. 1996. Characterization...

Andrew H. Lee; Lorraine S. Symington; David A. Fidock

2014-09-01T23:59:59.000Z

Note: This page contains sample records for the topic "double-strand break repair" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


101

Review of Gas Transmission Pipeline Repair Methods  

Science Journals Connector (OSTI)

Repair methods are key operations for the integrity management of pipelines. The parameters guiding the repair decision are briefly reminded. A nonexhaustive external and internal repair techniques are described,...

Remi Batisse

2008-01-01T23:59:59.000Z

102

Update on pipeline repair methods  

SciTech Connect

A comprehensive review of pipeline repair methods has been recently completed under the sponsorship of the American Gas Association`s, Pipeline Research Committee. This paper is intended to summarize the important results of that review. First and foremost, two relatively new methods of repair are reviewed. One involves the use of a continuous-fiber fiberglass composite material which can be applied as an alternative to a steel sleeve for the reinforcement of nonleaking defects. The second is the use of deposited weld metal to replace metal lost to external corrosion. This latter technique is not new in principle, but recent research has shown how it can be done safely on a pressurized pipeline. The other significant outcome of the comprehensive review was a set of guidelines for using all types of repairs including full-encirclement sleeves and repair clamps. Pipeline operators can use these guidelines to enhance their current repair procedures, or to train new personnel in maintenance techniques.

Kiefner, J.F. [Kiefner and Associates, Inc., Worthington, OH (United States); Bruce, W.A. [Edison Welding Inst., Columbus, OH (United States); Stephens, D.R. [Battelle, Columbus, OH (United States)

1995-12-31T23:59:59.000Z

103

Adhesive technologies in repairing polyethylene pipelines  

Science Journals Connector (OSTI)

Adhesive technologies for repairing polyethylene pipelines are considered taking into account the peculiarities ... the modified binder for application in the gluing repair technologies under consideration. It is...

V. F. Stroganov

2014-07-01T23:59:59.000Z

104

Restore: Modeling Repair and Restoration Processes | Argonne...  

NLE Websites -- All DOE Office Websites (Extended Search)

Restore: Modeling Repair and Restoration Processes Restore: Modeling Repair and Restoration Processes Argonne's Restore software models complex sets of steps required to accomplish...

105

Earth Repair: A Transatlantic History of Environmental Restoration  

E-Print Network (OSTI)

Earth Repair: A Transatlantic History of EnvironmentalHall. Earth Repair: A Transatlantic History of Environmental

Hamilton-Smith, Elery

2006-01-01T23:59:59.000Z

106

Coal bunker repairs  

SciTech Connect

Detroit Edison's St. Clair Power Plant (STCPP) Units 1 through 4 are 1950's vintage fossil fueled units with an average capacity of 163 megawatt per unit. Each unit had identical 2190 ton bunkers. The Unit No. 1 bunker had been experiencing noticeable exterior deterioration at the lower level internal support system. An internal bunker inspection revealed large deflections in the network of beams supporting the bunker side walls. A complete collapse of the internal support beams was imminent. Failure of these beams would have transferred the coal pressure loads to the bunker skin and external stiffeners which were not capable of sustaining the load and were also showing signs of distress. This paper presents the temporary repair installed immediately after inspection, the redesign of the lower internal support system and construction procedures involved in bringing the bunker back into operating condition.

Emmons, M.H.; Hoffman, M.G. (Detroit Edison Co., MI (United States))

1992-01-01T23:59:59.000Z

107

Electroweak Breaking in Supersymmetric Models  

E-Print Network (OSTI)

We discuss the mechanism for electroweak symmetry breaking in supersymmetric versions of the standard model. After briefly reviewing the possible sources of supersymmetry breaking, we show how the required pattern of symmetry breaking can automatically result from the structure of quantum corrections in the theory. We demonstrate that this radiative breaking mechanism works well for a heavy top quark and can be combined in unified versions of the theory with excellent predictions for the running couplings of the model. (To be published in ``Perspectives in Higgs Physics'', G. Kane editor.)

L. E. Ibanez; G. G. Ross

1992-04-01T23:59:59.000Z

108

Large steam turbine repair: A survey  

SciTech Connect

This report covers a survey taken to document the current state-of-the-art in repairs to large steam turbines. One objective was to provide information to assist utilities in making repair or replacement decisions. The survey revealed that a large number of repairs have been successfully repaired involving both mechanical and welding repair techniques. Repair techniques have been improving in recent years and are being used more frequently. No guidelines or codes exist for the repair of steam turbine components so each repair is primarily controlled by agreement between the utility, contractor and insurer. Types of repairs are reviewed in this report and in addition, the capabilities of various contractors who are currently active in providing repair service. 40 refs., 10 figs., 4 tabs.

Findlan, S.J.; Lube, B. (EPRI Nondestructive Evaluation Center, Charlotte, NC (United States))

1991-07-01T23:59:59.000Z

109

Selected Bibliography on Electric Motor Repair | Department of...  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Selected Bibliography on Electric Motor Repair Selected Bibliography on Electric Motor Repair The following series of repair documents related to electric motors were produced by...

110

Vanadium catalysts break down biomass for fuels  

NLE Websites -- All DOE Office Websites (Extended Search)

Vanadium catalysts break down biomass into useful components Breaking down biomass could help in converting biomass to fuels. March 26, 2012 Biomass Due to diminishing petroleum...

111

Breaking down the bubbly | EMSL  

NLE Websites -- All DOE Office Websites (Extended Search)

down the bubbly Breaking down the bubbly Micromodels redefine how bubbles characterize CO2 gas flow No, it's not a Lego: This Lab-on-a-Chip micromodel (left) was designed to...

112

Robotic equipment for pipeline repair  

SciTech Connect

Hyperbaric welding provides the most reliable method for connection or repair of subsea oil and gas pipelines. Research on hyperbaric arc welding processes indicates that it should be possible to achieve stable welding conditions with Gas Tungsten Arc (GTA) to approximately 600m, and with Gas Metal Arc (GMA) and Plasma Arc to at least 1,000m. These depths are well beyond the limits of manned saturation diving. At the present time the limitation on the maximum depth to which these processes can be applied, in practice, is the requirement for completely diverless operation deeper than approximately 350m. Fully diverless hyperbaric welding is not presently available to the industry but several diverless pipeline repair systems which utilize mechanical connectors have been developed. This paper reviews the present status of mechanized hyperbaric welding systems currently being used in the North Sea and discusses some of the work being done to achieve fully diverless robotic pipeline repair with both welding and connectors.

Gibson, D.E.; Barratt, K.; Paterson, J. [National Hyperbaric Centre, Aberdeen (United Kingdom)

1995-12-31T23:59:59.000Z

113

Technological Advances in Pipeline Isolation and Repair  

Science Journals Connector (OSTI)

Pipeline isolation has been practised for many years to implement various repairs. The tools originally applied were simple and...

Dr A. Aldeen

1994-01-01T23:59:59.000Z

114

Strongly Incremental Repair Detection Julian Hough  

E-Print Network (OSTI)

Strongly Incremental Repair Detection Julian Hough Dialogue Systems Group Faculty of Linguistics.purver@qmul.ac.uk Abstract We present STIR (STrongly Incremen- tal Repair detection), a system that de- tects speech repairs-theoretic measures from n-gram models as its principal decision features in a pipeline of classifiers detecting

Purver, Matthew

115

NERSC's Hopper Breaks Petaflops Barrier  

NLE Websites -- All DOE Office Websites (Extended Search)

NERSC's Hopper Breaks NERSC's Hopper Breaks Petaflops Barrier NERSC's Hopper Breaks Petaflops Barrier Ranks 5th in the World November 14, 2010 Media Contact: Jon Bashor, jbashor@lbl.gov, 510-486-5849 hopper1.jpg NERSC's Cray XE6-Hopper BERKELEY, Calif.-The Department of Energy's National Energy Research Scientific Computing Center (NERSC), already one of the world's leading centers for scientific productivity, is now home to the fifth most powerful supercomputer in the world and the second most powerful in the United States, according to the latest edition of the TOP500 list, the definitive ranking of the world's top computers NERSC's newest supercomputer, a 153,408 processor-core Cray XE6 system, posted a performance of 1.05 petaflops (quadrillions of calculations per second) running the Linpack benchmark. In keeping with NERSC's tradition of

116

Chiral Symmetry Breaking in Graphene  

E-Print Network (OSTI)

The question of whether the Coulomb interaction is strong enough to break the sublattice symmetry of un-doped graphene is discussed. We formulate a strong coupling expansion where the ground state of the Coulomb Hamiltonian is found exactly and the kinetic hopping Hamiltonian is treated as a perturbation. We argue that many of the properties of the resulting system would be shared by graphene with a Hubbard model interaction. In particular, the best candidate sublattice symmetry breaking ground state is an antiferromagnetic Mott insulator. We discuss the results of some numerical simulations which indicate that the Coulomb interaction is indeed subcritical. We also point out the curious fact that, if the electron did not have spin degeneracy, the tendency to break chiral symmetry would be much greater and even relatively weak Coulomb interactions would likely gap the spectrum.

Gordon W. Semenoff

2011-08-15T23:59:59.000Z

117

Dyes designed for high sensitivity detection of double-stranded DNA  

DOE Patents (OSTI)

Novel fluorescent dyes are provided, characterized by having a fluorophore joined to a cationic chain. The dyes are found to provide for high enhancement upon binding to nucleic acid and have strong binding affinities to the nucleic acid, as compared to the fluorophore without the polycationic chain. The dyes find use in detection of dsDNA in gel electrophoresis and solution at substantially higher sensitivities using substantially less dye.

Glazer, Alexander N. (Orinda, CA); Benson, Scott C. (Oakland, CA)

2000-01-01T23:59:59.000Z

118

Intense photoluminescence from dried double-stranded DNA and single-walled carbon nanotube hybrid  

SciTech Connect

Semiconducting single-walled carbon nanotubes (SWNTs) show near-infrared photoluminescence (PL) when they are individually isolated. This was an obstacle to use photonic properties of SWNTs on a solid surface. We show that SWNTs wrapped with DNA maintain intense PL under the dry conditions. SWNTs are well isolated individually by DNA even when the DNA-SWNT hybrids are agglomerated. This finding opens up application of SWNTs to photonic devices.

Ito, M.; Kobayashi, T.; Ito, Y.; Hayashida, T.; Nii, D.; Umemura, K.; Homma, Y. [Department of Physics, Tokyo University of Science, Tokyo 162-8601 (Japan)

2014-01-27T23:59:59.000Z

119

Dissection of Double-Stranded RNA Binding Protein B2 from Betanodavirus  

Science Journals Connector (OSTI)

...ahead of print on 21 March 2007. Beau J. Fenner Winnie Goh Jimmy Kwang Corresponding author...Mol. Biol. 279: 1085-1099. 10 Fenner, B. J., Q. Du, W. Goh, R. Thiagarajan...ELISA. J. Fish Dis. 29: 423-432. 11 Fenner, B. J., W. Goh, and J. Kwang...

Beau J. Fenner; Winnie Goh; Jimmy Kwang

2007-03-21T23:59:59.000Z

120

CTRLWORK AND MACBREAKZ ERGONOMIC BREAK REMINDER SOFTWARE  

E-Print Network (OSTI)

CTRLWORK AND MACBREAKZ ERGONOMIC BREAK REMINDER SOFTWARE SOFTWARE FEATURES: Analyzes your for installation assistance. 1. Please uninstall the former ergonomic break software, RSIGuard, if currently instructions on the screen. For more information, contact the UCSB Ergonomics Program at Ergonomics

California at Santa Barbara, University of

Note: This page contains sample records for the topic "double-strand break repair" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


121

The Novel Poly(ADP-Ribose) Polymerase Inhibitor, AG14361, Sensitizes Cells to Topoisomerase I Poisons by Increasing the Persistence of DNA Strand Breaks  

Science Journals Connector (OSTI)

...topoisomerase I as a resistance mechanism to camptothecin in tumour cells. Cancer Res 2001;61:5926-32. 46 El-Khamisy SF, Saifi GM, Weinfeld M, et al. Defective DNA single-strand break repair in spinocerebellar ataxia with axonal neuropathy. Nature...

Lisa M. Smith; Elaine Willmore; Caroline A. Austin; Nicola J. Curtin

2005-12-01T23:59:59.000Z

122

Sleeve installations speed pipeline defect repair  

SciTech Connect

Repairing defects in pipelines can be a major challenge for pipeline companies or contractors. To reduce cost and eliminate unscheduled shut downs, pipeline operating companies have adopted ``in-service`` repair methods to restore overall integrity of the pipeline without taking it out of service. Interprovincial Pipe Line Co. has undertaken an aggressive approach to this ``in-service`` repair method by using a developed sleeving system for repairing leaking and non-leaking defects. A structural reinforcement sleeve consists of two non-fillet welded collars (one on each side of the defect) and a full encirclement sleeve welded on top of these collars. The annular space between the pipe and sleeve is filled with a hardenable, non-shrinking epoxy. Three different pressure vessel sleeves can be used for repairing certain defects. They can be used in combination with the pre-stressed sleeve or for independent repairs. This paper reviews the performance and installation of these sleeves.

Friedrich, J.; Smith, J.

1995-12-01T23:59:59.000Z

123

Single molecule studies of DNA mismatch repair  

Science Journals Connector (OSTI)

Abstract DNA mismatch repair, which involves is a widely conserved set of proteins, is essential to limit genetic drift in all organisms. The same system of proteins plays key roles in many cancer related cellular transactions in humans. Although the basic process has been reconstituted in vitro using purified components, many fundamental aspects of DNA mismatch repair remain hidden due in part to the complexity and transient nature of the interactions between the mismatch repair proteins and DNA substrates. Single molecule methods offer the capability to uncover these transient but complex interactions and allow novel insights into mechanisms that underlie DNA mismatch repair. In this review, we discuss applications of single molecule methodology including electron microscopy, atomic force microscopy, particle tracking, FRET, and optical trapping to studies of DNA mismatch repair. These studies have led to formulation of mechanistic models of how proteins identify single base mismatches in the vast background of matched DNA and signal for their repair.

Dorothy A. Erie; Keith R. Weninger

2014-01-01T23:59:59.000Z

124

Breaking the Biological Barriers to Cellulosic Ethanol, June...  

Energy Savers (EERE)

Breaking the Biological Barriers to Cellulosic Ethanol, June 2006 Breaking the Biological Barriers to Cellulosic Ethanol, June 2006 Breaking the Biological Barriers to Cellulosic...

125

Repair Geometric Model by the Healing Method  

Science Journals Connector (OSTI)

Repairing the panel CAD geometric model is an ... and analyzed by NURBS technology. Through the pipeline method determines the matching-curves border fast...

Di Chi; Wang Weibo; Wen Lishu; Liu Zhaozheng

2012-01-01T23:59:59.000Z

126

Tests validate pipeline sleeve repair technique  

SciTech Connect

The sleeve-on-sleeve pipeline-repair technique is a viable, acceptable means of repairing sleeves and preventing fluid leakage resulting from fracture of cracked fillet welds at the ends of an existing single-layer sleeve. This technique was conceived by Interprovincial Pipe Line Co. as a means of repair for situations in which one or both of the circumferential fillet welds at the ends of an initial repair sleeve may need to be reinforced. It was necessary to determine whether this technique can be employed without serious impairment of the integrity of the pipelines on which it is to be used.

Kiefner, J.F.; Maxey, W.A. (Battelle Memorial Inst., Columbus, OH (USA))

1989-08-28T23:59:59.000Z

127

Crews repair transmission lines after Washington wildfire  

NLE Websites -- All DOE Office Websites (Extended Search)

Privacy Publications Tribal Affairs Newsroom Search News Articles... Search Crews repair transmission lines after Washington wildfire 662014 12:00 AM Tweet Page Content BPA...

128

Low Dose Radiation Research Program: Assessing Biological Function of DNA  

NLE Websites -- All DOE Office Websites (Extended Search)

Assessing Biological Function of DNA Damage Response Genes Assessing Biological Function of DNA Damage Response Genes Larry H. Thompson Lawrence Livermore National Laboratory Why This Project To understand the relative importance of individual DNA repair and DNA-damage response pathways to the recovery of mammalian cells after exposure to low doses of ionizing radiation (IR). This understanding may lead to better ways of setting limits on human exposure to IR. In spite of the discovery of many mammalian DNA repair genes, our current knowledge of how many of these genes contribute to cellular recovery from IR exposure is quite limited. Project Goals Measure cellular responses at doses in the 5-100 cGy range, which generally cause changes too small to detect in normal, repair-proficient cells Focus on DNA double-strand breaks (DSBs) and DNA oxidative base

129

Low Dose Radiation Research Program: Research Highlights - 2010  

NLE Websites -- All DOE Office Websites (Extended Search)

DNA Double-Strand Break Repair Capacities Before and After Exposure to Low DNA Double-Strand Break Repair Capacities Before and After Exposure to Low Dose Radiation Immunochemical detection of DSB foci in the nuclei of human fibroblasts. (A) γ-H2AX phospho-serine 139 foci (chromatin marker of DSBs; green). (B) ataxia-telangiectasia mutated phospho-serine 1981 foci (ATM, DNA damage-responsive kinase; red). (C) Merge of images A and B. (White arrows mark large γ-H2AX foci and coincident γ-H2AX/pATM foci that were positively scored; yellow arrows mark small γ-H2AX foci that lack corresponding pATM foci that were not scored.) Enlarge Image Immunochemical detection of DSB foci in the nuclei of human fibroblasts. (A) γ-H2AX phospho-serine 139 foci (chromatin marker of DSBs; green). (B) ataxia-telangiectasia mutated phospho-serine 1981 foci (ATM,

130

Direct Enzymatic Repair of Deoxyribonucleic Acid Single-Strand Breaks in Dormant Spores  

Science Journals Connector (OSTI)

...cesium-137 Gammator (Radiation Machinery Corp., Parsippany, N.J.). The dose rate was 100 or 300 krads...three tubes for each radiation dose were plated. 'TPhe...produced by more severe radiation doses in the exponential decline...

E. Durban; N. Grecz; J. Farkas

1974-04-01T23:59:59.000Z

131

Defective DNA single-strand break repair in spinocerebellar ataxia with axonal neuropathy-1  

Science Journals Connector (OSTI)

... in spinocerebellar ataxia with axonal neuropathy-1 Sherif F. El-Khamisy SF Gulam M.Saifi GM Michael Weinfeld M Fredrik Johansson F Thomas Helleday T James R. Lupski JR ...

Sherif F. El-Khamisy; Gulam M. Saifi; Michael Weinfeld; Fredrik Johansson; Thomas Helleday; James R. Lupski; Keith W. Caldecott

2005-03-03T23:59:59.000Z

132

International congress on DNA damage and repair: Book of abstracts  

SciTech Connect

This document contains the abstracts of 105 papers presented at the Congress. Topics covered include the Escherichia coli nucleotide excision repair system, DNA repair in malignant transformations, defective DNA repair, and gene regulation. (TEM)

Not Available

1987-01-01T23:59:59.000Z

133

Repair of overheating linear accelerator  

SciTech Connect

Los Alamos Neutron Science Center (LANSCE) is a proton accelerator that produces high energy particle beams for experiments. These beams include neutrons and protons for diverse uses including radiography, isotope production, small feature study, lattice vibrations and material science. The Drift Tube Linear Accelerator (DTL) is the first portion of a half mile long linear section of accelerator that raises the beam energy from 750 keV to 100 MeV. In its 31st year of operation (2003), the DTL experienced serious issues. The first problem was the inability to maintain resonant frequency at full power. The second problem was increased occurrences of over-temperature failure of cooling hoses. These shortcomings led to an investigation during the 2003 yearly preventative maintenance shutdown that showed evidence of excessive heating: discolored interior tank walls and coper oxide deposition in the cooling circuits. Since overheating was suspected to be caused by compromised heat transfer, improving that was the focus of the repair effort. Investigations revealed copper oxide flow inhibition and iron oxide scale build up. Acid cleaning was implemented with careful attention to protection of the base metal, selection of components to clean and minimization of exposure times. The effort has been very successful in bringing the accelerator through a complete eight month run cycle allowing an incredible array of scientific experiments to be completed this year (2003-2004). This paper will describe the systems, investigation analysis, repair, return to production and conclusion.

Barkley, Walter; Baldwin, William; Bennett, Gloria; Bitteker, Leo; Borden, Michael; Casados, Jeff; Fitzgerald, Daniel; Gorman, Fred; Johnson, Kenneth; Kurennoy, Sergey; Martinez, Alberto; O’Hara, James; Perez, Edward; Roller, Brandon; Rybarcyk, Lawrence; Stark, Peter; Stockton, Jerry

2004-01-01T23:59:59.000Z

134

Human embryonic stem cells for brain repair?  

Science Journals Connector (OSTI)

...repair compiled by Siddharthan Chandran, Maeve Caldwell and Nick Allen Human embryonic stem cells for brain repair? Su-Chun...stromal cell co-culture system. This type of technique has merits in its simplicity and the differentiated progenies appear to...

2008-01-01T23:59:59.000Z

135

NSLS Services | Repair & Equipment Services  

NLE Websites -- All DOE Office Websites (Extended Search)

Repair & Equipment Services Repair & Equipment Services Cleaning Facility (BNL Central Shops) Solvent cleaning of vacuum parts and leak checking service is also available. Work is billed to each user via a BNL ILR. Contact the NSLS Building Manager to arrange for any of these services. Electronics Repair Limited repairs for electronic equipment are available from an outside contractor through the Control Room. Contact Control Room Supervisor Randy Church (x2550 or x2736, pager 5310). Shipping memos are filled out on the web, and pick ups are on Fridays. Come to the Control Room for assistance. The user should call the contractor on the day before scheduled pickups to alert the contractor of the pickup. Items to be repaired should be left in the Control Room with the completed shipping memo. Costs are charged to the

136

Integrated structural repair of a producing FPSO  

SciTech Connect

The state of the art in FPSO design is advancing rapidly. The long-term reliability of FPSO systems has improved as maintenance issues, have received greater emphasis in both new-builds and conversions. Despite this new emphasis, problems will still arise and repairs will still be required. Ultimately, the ability of any FPSO to stay on location and on production will depend on the scope of repairs which can be economically performed in-situ. In 1994 and 1995, Marathon Petroleum Indonesia Limited (MPIL) performed an in-situ repair on the FPSO Kakap Natuna. The scope and complexity of this work suggests there are few, if any, limits on in-situ structural repairs which can be successfully performed on a producing FPSO. The use of an integrated execution strategy for the repairs greatly reduced their cost.

Johnson, P.R. [Marathon Oil Co., Houston, TX (United States); Smith, T.A. [Aker Omega Inc., Houston, TX (United States)

1997-07-01T23:59:59.000Z

137

Chapter 4 - Pipeline Inspection and Subsea Repair  

Science Journals Connector (OSTI)

Abstract Pipeline inspection is a part of the pipeline integrity management for keeping the pipeline in good condition. The rules governing inspection are the pipeline safety regulations. In most cases the pipeline is inspected regularly. The pipeline safety regulations require that the operator shall insure that a pipeline is maintained in an efficient state, in efficient working order and in good repair. The pipeline inspection includes external inspection and internal inspection. In this chapter, the metal loss inspection techniques are discussed. The subsea pipeline internal inspection is normally carried out through non-destructive testing techniques and technologies by intelligent pigs, such as magnetic-flux leakage technology inn axial and circumferential, ultrasound technologies, eddy-current technologies and other technologies. The repair methods are different for shallow and deep water subsea pipelines. The conventional repair methods are used for shallow water pipeline, but diverless repair and intelligent plus are good for deepwater pipeline repair.

Yong Bai; Qiang Bai

2014-01-01T23:59:59.000Z

138

INTERNAL REPAIR OF PIPELINES REVIEW & EVALUATION OF INTERNAL PIPELINE REPAIR TRIALS REPORT  

SciTech Connect

The two broad categories of fiber-reinforced composite liner repair and deposited weld metal repair technologies were reviewed and evaluated for potential application for internal repair of gas transmission pipelines. Both are used to some extent for other applications and could be further developed for internal, local, structural repair of gas transmission pipelines. Evaluation trials were conducted on pipe sections with simulated corrosion damage repaired with glass fiber-reinforced composite liners, carbon fiber-reinforced composite liners, and weld deposition. Additional un-repaired pipe sections were evaluated in the virgin condition and with simulated damage. Hydrostatic failure pressures for pipe sections repaired with glass fiber-reinforced composite liner were only marginally greater than that of pipe sections without liners, indicating that this type of liner is generally ineffective at restoring the pressure containing capabilities of pipelines. Failure pressure for pipe repaired with carbon fiber-reinforced composite liner was greater than that of the un-repaired pipe section with damage, indicating that this type of liner is effective at restoring the pressure containing capability of pipe. Pipe repaired with weld deposition failed at pressures lower than that of un-repaired pipe in both the virgin and damaged conditions, indicating that this repair technology is less effective at restoring the pressure containing capability of pipe than a carbon fiber-reinforced liner repair. Physical testing indicates that carbon fiber-reinforced liner repair is the most promising technology evaluated to-date. Development of a comprehensive test plan for this process is recommended for use in the next phase of this project.

Robin Gordon; Bill Bruce; Ian Harris; Dennis Harwig; George Ritter; Bill Mohr; Matt Boring; Nancy Porter; Mike Sullivan; Chris Neary

2004-09-01T23:59:59.000Z

139

Cadmium sulfate and CdTe-quantum dots alter DNA repair in zebrafish (Danio rerio) liver cells  

SciTech Connect

Increasing use of quantum dots (QDs) makes it necessary to evaluate their toxicological impacts on aquatic organisms, since their contamination of surface water is inevitable. This study compares the genotoxic effects of ionic Cd versus CdTe nanocrystals in zebrafish hepatocytes. After 24 h of CdSO{sub 4} or CdTe QD exposure, zebrafish liver (ZFL) cells showed a decreased number of viable cells, an accumulation of Cd, an increased formation of reactive oxygen species (ROS), and an induction of DNA strand breaks. Measured levels of stress defense and DNA repair genes were elevated in both cases. However, removal of bulky DNA adducts by nucleotide excision repair (NER) was inhibited with CdSO{sub 4} but not with CdTe QDs. The adverse effects caused by acute exposure of CdTe QDs might be mediated through differing mechanisms than those resulting from ionic cadmium toxicity, and studying the effects of metallic components may be not enough to explain QD toxicities in aquatic organisms. - Highlights: • Both CdSO{sub 4} and CdTe QDs lead to cell death and Cd accumulation. • Both CdSO{sub 4} and CdTe QDs induce cellular ROS generation and DNA strand breaks. • Both CdSO{sub 4} and CdTe QDs induce the expressions of stress defense and DNA repair genes. • NER repair capacity was inhibited with CdSO{sub 4} but not with CdTe QDs.

Tang, Song; Cai, Qingsong [The Institute of Environmental and Human Health, Texas Tech University, Lubbock, TX 79416 (United States); Chibli, Hicham [Department of Biomedical Engineering, McGill University, Montréal, QC H3A 2B4 (Canada); Allagadda, Vinay [The Institute of Environmental and Human Health, Texas Tech University, Lubbock, TX 79416 (United States); Nadeau, Jay L. [Department of Biomedical Engineering, McGill University, Montréal, QC H3A 2B4 (Canada); Mayer, Gregory D., E-mail: greg.mayer@ttu.edu [The Institute of Environmental and Human Health, Texas Tech University, Lubbock, TX 79416 (United States)

2013-10-15T23:59:59.000Z

140

Influence of old rectangular repair patches on the burst pressure of a gas pipeline  

Science Journals Connector (OSTI)

Seven full scale hydrostatic burst tests were carried out on pipes extracted from an API 5LX52 gas pipeline that contained rectangular and elliptical fillet welded patches and other repairs of different geometries. All breaks took place after widespread yielding. This analysis shows that the patches that generate greater risks are those that: (1) were attached to the pipeline at very low pressure, (2) were placed to repair large defects, (3) are rectangular, long in the direction of the pipe, and narrow, (4) the quality of the weld is doubtful. Based on data reported by In Line Inspection (ILI), of the four conditions mentioned above, only the third can be assessed in order to quantify risks and to schedule replacements.

Pablo Gabriel Fazzini; José Luis Otegui

2006-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "double-strand break repair" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


141

Diverless pipeline repair clamp: Phase 1  

SciTech Connect

Offshore oil and gas developments are underway for water depths beyond which divers can function. The economic lifelines of these projects are the pipelines which will transport the products to shore. In preparation for the day when one of these pipelines will require repair because of a leak, the Pipeline Research Committee of the American Gas Association is funding research directed at developing diverless pipeline repair capabilities. Several types of damage are possible, ranging from latent weld defects on one end of the spectrum to damage resulting in parting of the pipe at the other end. This study is specifically directed toward laying the groundwork for development of a diverless pipeline repair clamp for use in repair of leaks resulting from minor pipe defects. The incentive for a clamp type repair is costs. When compared to replacing a section of pipe, either by welding or by mechanical means, the clamp type repair requires much less disturbance of the pipe, less time, fewer operations and less equipment. This report summarizes (1) capabilities of remotely operated vehicles (ROV's) and associated systems, (2) highlights areas for further research and development, (3) describes the required capabilities of the diverless repairclamp, (4) investigates some alternatives to the diverless clamp, (5) overviews the state of the art in leak repair clamps, and (6) critiques several possible generic clamp concepts.

Miller, J.E.; Knott, B. (Stress Engineering Services, Inc., Houston, TX (United States))

1991-12-01T23:59:59.000Z

142

Deepwater pipeline repair technology: A general overview  

SciTech Connect

During the life of oil and gas transportation sea lines, periodic inspection, maintenance and repair in case of major damage are the most important tasks to be considered especially in deepwater installations. In particular the capabilities to perform quick and cost effective repairs have been of strategic importance in the eighties for SNAM during the development of the S.A.S. (Submersible Automatic System) a diverless and guidelineless repair system for the 20 inch Transmediterranean sealines. The trials on this prototype were successfully completed in early summer 1992, simulating a complete repair procedure at 610 in water depth. Based on the technology the authors have acquired during the implementation of the system, an upgrading phase aimed at improving the capability to mate the new 26 inch lines is being developed. Considering that at the moment only a few pipeline transportation systems are laid in deep water, but some new installations are foreseen in the near future, technological developments would be necessary in view of different scenarios other than the Mediterranean area. This paper will be focused on an overview of the existing repair technologies and will discuss the possible future pipelines operating scenarios and the envisaged new developments of repair technology. Possible way of approaching and solving in a cost-effective way the needs of Pipeline Operators to have repair systems available will be discussed for the different pipeline scenarios.

Magnelli, G.; Radicioni, A. [Snamprogetti S.p.A., Fano (Italy). Offshore Division

1994-12-31T23:59:59.000Z

143

Issues in standard model symmetry breaking  

SciTech Connect

This work discusses the symmetry breaking sector of the SU(2) x U(1) electroweak model. The first two chapters discuss Higgs masses in two simple Higgs models. The author proves low-enery theorems for the symmetry breaking sector: The threshold behavior of gauge-boson scattering is completely determined, whenever the symmetry breaking sector meets certain simple conditions. The author uses these theorems to derive event rates for the superconducting super collider (SSC). The author shows that the SSC may be able to determine whether the interactions of the symmetry breaking sector are strong or weak. 54 refs.

Golden, M.

1988-04-01T23:59:59.000Z

144

Developments advance subsea pipelaying, inspection, repair  

SciTech Connect

Recent advances in laying, inspecting, and repairing pipelines are helping to cut both costs and time. A new dredging system that employs jets to clear a subsea trench for pipelay received trials off Belgium last spring. Also, within the last year, projects in the Middle East and North Sea employed technologies that promise to make inspecting the surface of a subsea pipeline in difficult terrain easier, less time consuming, and therefore less costly. Plus, subsea repair of damaged pipelines may take less time with a new ``stabbable`` pipe connector. The paper describes jet dredging, inspection advance, support software, the North Sea site, and pipeline repair.

NONE

1997-09-15T23:59:59.000Z

145

System Break-Out Session  

NLE Websites -- All DOE Office Websites (Extended Search)

Alkaline Membrane Fuel Cell Alkaline Membrane Fuel Cell Workshop System Break-Out Session Anil Trehan (CommScope) Huyen Dinh (NREL) 5/8 - 5/9/11 Washington DC Current State-of-art Performance * MEA level - 200 mW/cm2 peak power at 0.5 V, 60-65C * System level - Air-cooled 2 kW (net) - Energy density is advantage over incumbent battery and Gensets for stationary application - Significant price advantage over PEM fuel cells * Refer to CellEra and Tokuyama presentations for more details Application - Alcohol fuel small power levels Application Description Power range Military Remote sensor < 10 W Soldier Power 20- 50 W Battery charger 300 W Commercial Consumer electronics < 100 W Recreation < 500 W Commercial Application - Hydrogen Description Power range Back-up 1 - 10 kW Material Handling 1 - 10 kW Transportation

146

MDM2 Is Required for Suppression of Apoptosis by Activated Akt1 in Salivary Acinar Cells  

Science Journals Connector (OSTI)

...serves to hydrolyze starches to simple sugars (97). Expression of amylase was analyzed in all...double-stranded DNA breaks (84), and gamma...suggest that the down-regulation of p53...Biochemistry of human alpha amylase isoenzymes. Crit...

Kirsten H. Limesand; Kathryn L. Schwertfeger; Steven M. Anderson

2006-09-18T23:59:59.000Z

147

E-Print Network 3.0 - adjacent pericarcinomatous foci Sample...  

NLE Websites -- All DOE Office Websites (Extended Search)

p53 binding protein 1 foci as a biomarker of DNA double strand breaks induced by ionizing radiation... Radiation effect Bysta effect 53 BP1 a b s t r a c t Foci of p53 binding...

148

Joint Characterisation for Repair Brazing of Superalloys  

Science Journals Connector (OSTI)

The restricted weldability of superalloys that can operate at high temperatures and under mechanical loadings, required in modern gas turbines, has lead to the development of repair brazing techniques for vari...

L. S. K. Heikinheimo; A. Laukkanen; J. Veivo

2005-05-01T23:59:59.000Z

149

Encirclement sleeves reduce pipeline repair costs  

SciTech Connect

Welded sleeve, or replacement of line repair methods have been used successfully for many years in the pipeline industry but can lead to other difficulties for a pipeline operator. Clock Spring`s composite sleeves have been used in over ten thousand pipeline repairs with pipe sizes ranging from 6- to 56-inches in diameter, all without costly shutdown, welding or purging. Repairs can be completed while the pipeline is fully operational and require only six inches of clearance under the pipe for wrapping the eight thicknesses of the coil. This minimizes costly digging and backfilling over long runs of pipe and necessary shoring for personnel safety. Also it provides a more cost-effective alterative to conventional pipeline repair since special handling, lifting, or installation equipment is not needed. This paper reviews the installation and performance of these sleeves.

NONE

1996-01-01T23:59:59.000Z

150

A fluorescence method to detect photo-activated repair of UV-induced DNA damage in marine organisms  

SciTech Connect

After UV exposure, marine microorganisms may re-activate their photosynthetic systems or regain their pre-exposure metabolic rates, but they will not long survive, nor grow or reproduce, if they cannot repair DNA or ERN damage. Several repair mechanisms, including nucleotide excision repair and photo-reactivation, are known in terrestrial organisms, but there is little information on either process in marine microorganisms. Photo-reactivation may be especially important near the ocean surface where a few meters difference in vertical position or a few hours difference in time may alleviate UV-irradiation stress yet still provide sufficient light for photoactivated repair processes. We have developed a relatively simple, sensitive method to measure the activity of the photo-reactivation enzyme, photolyase, by fluorometry. UV irradiation causes adjacent thymine nucleotides in DNA to form cyclobutane dimers. Photolyase attaches to the dimerized DNA then breaks the dimers in a reaction catalyzed by visible light, thereby allowing restoration of cross-strand base pairing. Our assay for photolyase activity involves a dimerized synthetic DNA and DNA-specific fluorochromes with affinity for thymine-rich regions of DNA. When thymine dimers are present, fluorescence of these dyes is reduced. As dimers are repaired, fluorescence increases, allowing us to demonstrate the presence of photolyase and measure its activity using conventional fluorometers available in most marine laboratories. Photo-reactivation activity has been unambiguously demonstrated in samples of coastal seawater. In preliminary investigations, cells taken from surface slicks did not have enhanced photo-repair capabilities relative to cells collected 5 meters below the surface. Additional tests of the method and measurements of photo-repair activities of surface and subsurface organisms will be made during the SLIX-89 Surface Microlayer Experiment in October 1989.

Carlson, D.J.; Mordy, C.W.; Nelson, K. (Oregon State Univ., Corvallis (United States))

1990-01-09T23:59:59.000Z

151

Explicit CP Breaking and Electroweak Baryogenesis  

Science Journals Connector (OSTI)

......1996 research-article Articles Explicit CP Breaking and Electroweak Baryogenesis Koichi...We investigate spatial behaviors of the CP-violating angle theta by solving the equation...model in the presence of a small explicit CP breaking delta. The moduli of the two Higgs......

Koichi Funakubo; Akira Kakuto; Shoichiro Otsuki; Fumihiko Toyoda

1996-10-01T23:59:59.000Z

152

Vanadium catalysts break down biomass for fuels  

E-Print Network (OSTI)

- 1 - Vanadium catalysts break down biomass for fuels March 26, 2012 Vanadium catalysts break down biomass into useful components Due to diminishing petroleum reserves, non-food biomass (lignocellulose biomass into high-value commodity chemicals. The journal Angewandte Chemie International Edition published

153

Application of composite repair for pipeline anomalies  

SciTech Connect

The cost of maintaining the structural integrity of the 650,000 kilometer high-pressure gas gathering and transmission pipeline network is a significant part of the operating budget of the US pipeline industry. To help in controlling thee costs, the Gas Research institute (GRI) has supported research resulting in the development of Clock Spring{reg_sign}, a low-cost fiberglass composite alternative to conventional steel sleeves for transmission line pipe reinforcement and repair. Investigation and development of engineering guidelines have been completed. Field validation of laboratory research on application of Clock Spring as a repair for corrosion and mechanical damage defects is in progress. This paper presents an overview of composite repair technology for pipeline corrosion and mechanical damage defects. It summarizes the results and conclusions of modeling and experiments on reinforcement and repair of both corrosion and mechanical damage (i.e., dent and gouge) pipeline defects. These investigations provide quantitative results on the operating envelope of composite reinforcements and installation requirements that ensure sound and reliable repair of pipeline defects. The paper further summarizes the work to date on field installation in verification of composite repair performance.

Stephens, D.R. [Battelle, Columbus, OH (United States); Lindholm, U.S. [Southwest Research Inst., San Antonio, TX (United States); Hill, V.L. [Gas Research Inst., Chicago, IL (United States); Block, N. [Clock Spring Co., Houston, TX (United States)

1996-09-01T23:59:59.000Z

154

Vanadium catalysts break down biomass for fuels  

NLE Websites -- All DOE Office Websites (Extended Search)

Vanadium catalysts break down biomass for fuels Vanadium catalysts break down biomass for fuels Vanadium catalysts break down biomass into useful components Breaking down biomass could help in converting biomass to fuels. March 26, 2012 Biomass Due to diminishing petroleum reserves, non-food biomass (lignocellulose) is an attractive alternative as a feedstock for the production of renewable chemicals and fuels. Get Expertise Researcher Susan Hanson Inorganic Isotope & Actinide Chem Email Researcher Ruilian Wu Bioenergy & Environmental Science Email Researcher Louis "Pete" Silks Bioenergy & Environmental Science Email Vanadium is an inexpensive, earth-abundant metal that is well suited for promoting oxidations in air. Vanadium catalysts break down biomass into useful components Due to diminishing petroleum reserves, non-food biomass (lignocellulose) is

155

Energy and Technology Review: Unlocking the mysteries of DNA repair  

SciTech Connect

DNA, the genetic blueprint, has the remarkable property of encoding its own repair following diverse types of structural damage induced by external agents or normal metabolism. We are studying the interplay of DNA damaging agents, repair genes, and their protein products to decipher the complex biochemical pathways that mediate such repair. Our research focuses on repair processes that correct DNA damage produced by chemical mutagens and radiation, both ionizing and ultraviolet. The most important type of DNA repair in human cells is called excision repair. This multistep process removes damaged or inappropriate pieces of DNA -- often as a string of 29 nucleotides containing the damage -- and replaces them with intact ones. We have isolated, cloned, and mapped several human repair genes associated with the nucleotide excision repair pathway and involved in the repair of DNA damage after exposure to ultraviolet light or mutagens in cooked food. We have shown that a defect in one of these repair genes, ERCC2, is responsible for the repair deficiency in one of the groups of patients with the recessive genetic disorder xeroderma pigmentosum (XP group D). We are exploring ways to purify sufficient quantities (milligrams) of the protein products of these and other repair genes so that we can understand their functions. Our long-term goals are to link defective repair proteins to human DNA repair disorders that predispose to cancer, and to produce DNA-repair-deficient mice that can serve as models for the human disorders.

Quirk, W.A.

1993-04-01T23:59:59.000Z

156

Radiation Induced Chromatid-type Aberrations after Irradiation of Late-S/G2  

NLE Websites -- All DOE Office Websites (Extended Search)

Induced Chromatid-type Aberrations after Irradiation of Late-S/G2 Induced Chromatid-type Aberrations after Irradiation of Late-S/G2 Cells: Roles of Homologous Recombination and Non-Homologous End Joining Joel Bedford Colorado State University Abstract There has been considerable discussion, with some data reported, addressing the question of the relative contributions of non-homologous end joining (NHEJ) and homologous recombinational repair (HRR) on the repair or rejoining of radiation-induced DNA double strand breaks, especially in relation to their operation during the cell cycle. Reports have included studies on chromosomal aberration induction in G1/G0 cells defective in NHEJ or A-T cells, for example, but relatively little on S/G2 cells and especially on mutants defective in HRR. The broad biological importance of

157

E-Print Network 3.0 - auto body repair Sample Search Results  

NLE Websites -- All DOE Office Websites (Extended Search)

"repairable assets... of which can be plugged into SAWA. Figure 1. Repairable Assets Pipeline 2 Repairable Assets ... Source: Baclawski, Kenneth B. - College of Computer...

158

Transcriptional and Epigenetic Responses of Human Cells to Low Dose  

NLE Websites -- All DOE Office Websites (Extended Search)

Transcriptional and Epigenetic Responses of Human Cells to Low Dose Transcriptional and Epigenetic Responses of Human Cells to Low Dose Ionizing Radiation Identified through High Throughput ChIP-Seq Analysis Carl Anderson Brookhaven National Laboratory Abstract The major consequence of human exposures to ionizing radiation (IR) is considered to be an increased incidence of cancer (Brenner et al., 2003). Exposure of cells to 1 Gy of IR produces approximately 40 double-stranded breaks, 1000 single-stranded breaks, and 1000 damaged bases per genome equivalent (Pandita and Richardson, 2009); however, most direct DNA damage is rapidly repaired. Exposure to IR also induces epigenetic changes including both increases and decreases in DNA methylation, and increasing evidence suggests that epigenetic changes can both initiate cancer and

159

Repair of mismatched basepairs in mammalian DNA  

SciTech Connect

We have concentrated on three specific areas of our research plan. Our greatest emphasis is on the role of single strand nicks in influencing template strand selection in mismatch repair. We have found, that the ability of a nick in one strand to influence which strand is repaired is not a simple function of distance from the mismatched site but rather that an hot spot where a nick is more likely to have an influence can exist. The second line was production of single-genotype heteroduplexes in order to examine independently the repair of T/G and A/C mispairs within the same sequence context as in our mixed mispair preparations. We have shown preparations of supercoiled heteroduplex can be prepared that were exclusively T/G or exclusively A/C at the mispair site. The third effort has been to understand the difference in repair bias of different cell lines or different transfection conditions as it may relate to different repair systems in the cell. We have identified some of the sources of variation, including cell cycle position. We hope to continue this work to more precisely identify the phase of the cell cycle.

Taylor, J.H.; Hare, J.T.

1991-08-01T23:59:59.000Z

160

Fabrication Flaws in Reactor Pressure Vessel Repair Welds  

SciTech Connect

This paper describes the fabrication flaw distribution and characterization in the repair weld metal of reactor pressure vessels. This work indicates that the large flaws occur in these repairs. These results show that repair flaws are complex in composition and sometimes include cracks on the repair ends. Parametric analysis using an exponential fit is performed on the data. A description of repair flaw morphology is provided. Fabrication flaws in repairs are characterized using high sensitivity nondestructive ultrasonic testing, validation by other nondestructive evaluation (NDE) techniques, and complemented by destructive testing.

Schuster, George J.; Doctor, Steven R.

2007-12-01T23:59:59.000Z

Note: This page contains sample records for the topic "double-strand break repair" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


161

Capillary Break Beneath a Slab: Polyethylene Sheeting over Aggregate...  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

Capillary Break Beneath a Slab: Polyethylene Sheeting Over Aggregate Southwestern Pennsylvania PROJECT INFORMATION Project Name: Capillary Break Beneath a Slab: Polyethylene...

162

Breaking the Fuel Cell Cost Barrier | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Breaking the Fuel Cell Cost Barrier Breaking the Fuel Cell Cost Barrier Presentation at the AMFC Workshop, May 8, Arlington, VA amfc050811gottesfeldcellera.pdf More Documents &...

163

Regional Test Centers Breaking Down Barriers to Solar Energy...  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Regional Test Centers Breaking Down Barriers to Solar Energy Deployment Regional Test Centers Breaking Down Barriers to Solar Energy Deployment September 3, 2014 - 6:16pm Addthis...

164

Airborne observations of the kinematics and statistics of breaking waves  

E-Print Network (OSTI)

v 3 Observations of wave breaking kinematics in fetch-crest length . . . . . C.6 Wave elevation . . . . . . . .breaking waves in the images . . . . . . . . . . . 3.3.3

Kleiss, Jessica M.

2009-01-01T23:59:59.000Z

165

Wave Breaking Dissipation Observed with “SWIFT” Drifters  

Science Journals Connector (OSTI)

Energy dissipation rates during ocean wave breaking are estimated from high-resolution profiles of turbulent velocities collected within 1 m of the surface. The velocity profiles are obtained from a pulse-coherent acoustic Doppler sonar on a wave-...

Jim Thomson

2012-12-01T23:59:59.000Z

166

Ignition with Laser Break-Down  

Science Journals Connector (OSTI)

It is considered that ignition with laser break-down is one of the applications of solid-state lasers. This paper shows basic experimental results indicating the advantages of laser...

Furutani, Hirohide; Saito, Takeshi

167

Repairable chip bonding/interconnect process  

DOE Patents (OSTI)

A repairable, chip-to-board interconnect process which addresses cost and testability issues in the multi-chip modules. This process can be carried out using a chip-on-sacrificial-substrate technique, involving laser processing. This process avoids the curing/solvent evolution problems encountered in prior approaches, as well is resolving prior plating problems and the requirements for fillets. For repairable high speed chip-to-board connection, transmission lines can be formed on the sides of the chip from chip bond pads, ending in a gull wing at the bottom of the chip for subsequent solder.

Bernhardt, Anthony F. (Berkeley, CA); Contolini, Robert J. (Livermore, CA); Malba, Vincent (Livermore, CA); Riddle, Robert A. (Tracy, CA)

1997-01-01T23:59:59.000Z

168

Tank Remote Repair System Conceptual Design  

SciTech Connect

This document describes two conceptual designs for a Tank Remote Repair System to perform leak site repairs of double shell waste tank walls (Types I, II, III, and IIIA) from the annulus space. The first concept uses a magnetic wall crawler and an epoxy patch system and the second concept uses a magnetic wall crawler and a magnetic patch system. The recommended concept uses the magnetic patch system, since it is simpler to deliver, easier to apply, and has a higher probability of stopping an active leak.

Kriikku, E.

2002-12-06T23:59:59.000Z

169

Development of a composite repair system for reinforcing offshore risers  

E-Print Network (OSTI)

A research program was conducted to investigate the application of composite materials in repairing corroded offshore risers, leading to the development of an optimized repair using a hybrid carbon/E-glass system. The objective of this research...

Alexander, Christopher Richard

2009-05-15T23:59:59.000Z

170

In-service repair of main pipelines by welding  

Science Journals Connector (OSTI)

A new approach to the repair of main pipelines by welding without removing them from service ... failure risk; safety of welding works on pipeline under pressure; use of different variants of repair by welding; s...

V. I. Makhnenko; V. S. But; O. I. Oleinik

2009-09-01T23:59:59.000Z

171

Role of Lon, an ATP-Dependent Protease Homolog, in Resistance of Pseudomonas aeruginosa to Ciprofloxacin  

Science Journals Connector (OSTI)

...in the pharmaceutical pipeline, resistance to the current...of SOS-mediated DNA repair mechanisms and cell filamentation...PA4763 recN RecN DNA repair protein H1121 6_G1...faithful recombinational repair of DNA double-strand...in the pharmaceutical pipeline, resistance to the current...

Michelle D. Brazas; Elena B. M. Breidenstein; Joerg Overhage; Robert E. W. Hancock

2007-09-24T23:59:59.000Z

172

Predicting cisplatin and trabectedin drug sensitivity in ovarian and colon cancers  

Science Journals Connector (OSTI)

...www.gene.com/gene/pipeline/status/oncology/apo2l...to broken chromosomes.DNA Repair (Amst) 2006;5:534-43...19. Lukas J Bartek J.DNA repair: New tales of an old tail...depends on nucleotide excision repair and not DNA double-strand...

Ellen V. Stevens; Satoshi Nishizuka; Smitha Antony; Mark Reimers; Sudhir Varma; Lynn Young; Peter J. Munson; John N. Weinstein; Elise C. Kohn; and Yves Pommier

2008-01-01T23:59:59.000Z

173

Improving the Design Reliability of Petroleum Pipeline Components on Repair  

Science Journals Connector (OSTI)

Developments are considered that may appreciably improve the design reliability in the repair of petroleum pipeline components.

I. N. Karelin

174

Strongly Incremental Repair Detection Julian Hough1,2  

E-Print Network (OSTI)

in a pipeline of classifiers detecting the the different stages of repairs. Results on the Switchboard disStrongly Incremental Repair Detection Julian Hough1,2 1 Dialogue Systems Group Faculty of London m.purver@qmul.ac.uk Abstract We present STIR (STrongly Incremen- tal Repair detection), a system

Purver, Matthew

175

Pipeline incidents and emergency repair in the North Sea  

SciTech Connect

The failures of submarine pipelines in the North Sea, and the response of pipeline operators are first discussed. Against this background, the methods currently available for submarine pipeline repairs are reviewed. The Emergency Pipeline Repair Services available are described, and some future developments in the field of submarine pipeline repair are briefly outlined.

Wood, G.D.

1988-12-01T23:59:59.000Z

176

New Mechanisms of Gauge-Mediated Supersymmetry Breaking  

E-Print Network (OSTI)

We review the motivation for Gauge-Mediated Supersymmetry Breaking and discuss some recent advances.

Lisa Randall

1997-06-23T23:59:59.000Z

177

The Effect of Wave Breaking on the Wave Energy Spectrum  

Science Journals Connector (OSTI)

The effect of wave breaking on the wave energy spectral shape is examined. The Stokes wave-breaking criterion is first extended to random waves and a breaking wave model is established in which the elevation of breaking waves is expressed in ...

C. C. Tung; N. E. Huang

1987-08-01T23:59:59.000Z

178

Conformal Supersymmetry Breaking and Dynamical Tuningof the Cosmological Constant  

SciTech Connect

We propose 'conformal supersymmetry breaking' models, which tightly relate the conformal breaking scale (i.e. R-symmetry breaking scale) and the supersymmetry breaking scale. The both scales are originated from the constant term in the superpotential through the common source of the R-symmetry breaking. We show that dynamical tuning between those mass scales significantly reduces the degree of fine-tuning necessary for generating the almost vanishing cosmological constant.

Ibe, M.; /SLAC; Nakayama, Y.; /UC, Berkeley; Yanagida, T.T.; /Tokyo U.

2008-03-07T23:59:59.000Z

179

Breaking Down Stovepipes | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Breaking Down Stovepipes Breaking Down Stovepipes Breaking Down Stovepipes Coordination with other DOE-wide teams and groups An assigned liaison to DOE's NEPA [National Environmental Policy Act] Six Sigma Team Coordination with the Facilities and Infrastructure Steering Committee: Ten-Year Site Plan requirements, streamlining processes for transferring facilities, work for others Communication across sites, programs, and communities to share lessons learned Kansas City Office coordinating with Oak Ridge and the Office of Environmental Management on the Bannister Federal Complex disposal lessons learned Office of Science sharing lessons on private industry opportunities to expand research capabilities at Oak Ridge and Brookhaven National Lab and on the use of opportunities offered by local government

180

The breaking strain of neutron star crust  

SciTech Connect

Mountains on rapidly rotating neutron stars efficiently radiate gravitational waves. The maximum possible size of these mountains depends on the breaking strain of neutron star crust. With multimillion ion molecular dynamics simulations of Coulomb solids representing the crust, we show that the breaking strain of pure single crystals is very large and that impurities, defects, and grain boundaries only modestly reduce the breaking strain to around 0.1. Due to the collective behavior of the ions during failure found in our simulations, the neutron star crust is likely very strong and can support mountains large enough so that their gTavitational wave radiation could limit the spin periods of some stars and might be detectable in large scale interferometers. Furthermore, our microscopic modeling of neutron star crust material can help analyze mechanisms relevant in Magnetar Giant and Micro Flares.

Kadau, Kai [Los Alamos National Laboratory; Horowitz, C J [INDIANA UNIV

2009-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "double-strand break repair" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


181

Modelling oil price volatility with structural breaks  

Science Journals Connector (OSTI)

In this paper, we provide two main innovations: (i) we analyze oil prices of two prominent markets namely West Texas Intermediate (WTI) and Brent using the two recently developed tests by Narayan and Popp (2010) and Liu and Narayan, 2010 both of which allow for two structural breaks in the data series; and (ii) the latter method is modified to include both symmetric and asymmetric volatility models. We identify two structural breaks that occur in 1990 and 2008 which coincidentally correspond to the Iraqi/Kuwait conflict and the global financial crisis, respectively. We find evidence of persistence and leverage effects in the oil price volatility. While further extensions can be pursued, the consideration of asymmetric effects as well as structural breaks should not be jettisoned when modelling oil price volatility.

Afees A. Salisu; Ismail O. Fasanya

2013-01-01T23:59:59.000Z

182

Gauge - Mediated Supersymmetry Breaking in String Compactifications  

SciTech Connect

We provide string theory examples where a toy model of a SUSY GUT or the MSSM is embedded in a compactification along with a gauge sector which dynamically breaks supersymmetry. We argue that by changing microscopic details of the model (such as precise choices of flux), one can arrange for the dominant mediation mechanism transmitting SUSY breaking to the Standard Model to be either gravity mediation or gauge mediation. Systematic improvement of such examples may lead to top-down models incorporating a solution to the SUSY flavor problem.

Diaconescu, Duiliu-Emanuel; /Rutgers U., Piscataway; Florea, Bogdan; Kachru, Shamit; Svrcek, Peter; /Stanford U., Phys. Dept. /SLAC

2006-01-04T23:59:59.000Z

183

Actions to promote energy-efficient electric motor repair  

Science Journals Connector (OSTI)

Electric motors are repaired 2-3 times over their lifetime. Poor repair practices, particularly widespread in developing countries, can lead to a significant increase in motor losses. It is estimated that worldwide poor repair leads to annual electricity losses of approximately 40 TWh, representing 20 Mtons of CO2 emissions. The main objective of this paper is to provide a technical basis for designing demand side management actions, which address the motor repair market. This paper brings into focus motor repair process trends, energy-efficient motors, typical repair process, actions to promote energy-efficient motor repair, energy-efficient motor rebate schemes to replace old, badly damaged motors and the factors influencing payback. This paper will be useful to energy policy makers, and Demand Side Management (DSM) staff in energy agencies and electric utilities.

Aníbal T. de Almeida; Fernando J.T.E. Ferreira

2003-01-01T23:59:59.000Z

184

Spontaneous symmetry breaking in gauge theories  

Science Journals Connector (OSTI)

...and beyond the discovery of the Higgs boson. organised and edited by John...and beyond the discovery of the Higgs boson . The aim of this historical article...whose features is the now-famous Higgs boson. symmetry breaking|gauge theory...

2015-01-01T23:59:59.000Z

185

Void fraction measurements in breaking waves  

Science Journals Connector (OSTI)

...height H b and depth d b, energy dissipated per unit crest length in each breaking...vortex area A v. The energy dissipated was obtained from measurements of the incident, transmitted...transmitted and reflected wave energies per unit surface area, respectively...

2007-01-01T23:59:59.000Z

186

REPLACE YOUR MERCURY THERMOMETERS BEFORE THEY BREAK!  

E-Print Network (OSTI)

REPLACE YOUR MERCURY THERMOMETERS BEFORE THEY BREAK! Did you know, mercury from broken thermometers to the local environment, if broken thermometers in sinks eventually end at the sanitary sewer plant. Broken mercury thermometers create hazardous waste that is costly to clean up and costly to dispose of. Other

187

Natural and synthetic double-stranded DNA binding studies of macrocyclic tetraamine zinc(II) complexes appended with polyaromatic groups  

Science Journals Connector (OSTI)

...?The characteristic binding mode of zinc(II) complexes of macrocyclic tetraamines (1,4,7,10-tetraazacyclododecane, cyclen) appended with one or two arylmethyl group(s) [(4-quinolyl)methyl-, 1,7-bis(4-quinolyl)...

Emiko Kikuta; Naomi Katsube; E. Kimura

1999-08-01T23:59:59.000Z

188

Unzipping Kinetics of Double-Stranded DNA in a Nanopore Alexis F. Sauer-Budge,1,2  

E-Print Network (OSTI)

- technological applications [11,12]. To explore strand separation in a nanopore we designed two synthetic DNA

189

Algae create glue to repair cell damage  

Science Journals Connector (OSTI)

... The reproductive secret of an environmentally damaging alga has been rumbled. When strands of Caulerpa taxifolia break off to form separate organisms, ... organisms, the parent heals itself with a fast-acting natural glue. This helps the algae to spread rapidly, but might also prove an Achilles heel, aiding the ecologists fighting ...

Mark Peplow

2005-03-23T23:59:59.000Z

190

Distribution System Audits, Leak Detection, and Repair: Kirtland Air Force Base Leak Detection and Repair Program  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Best Management Practice Best Management Practice Case Study #3 Distribution System Audits, Leak Detection, and Repair Kirtland Air Force Base - Leak Detection and Repair Program Overview Kirtland Air Force Base (AFB) performed an award winning leak detection and repair program in 2006. The results of the project are saving Kirtland AFB 179 million gallons each year, which is over 16% of the total water use at the base. Kirtland AFB is located on 52,000 acres, southeast and adjacent to Albuquerque, New Mexico. The area is a high altitude desert, only receiving about 8 inches of rain each year. Kirtland AFB draws water from an under- ground aquifer via seven production wells through- out the base. The base also has access to water from the City of Albuquerque. The underground water

191

Distribution System Audits, Leak Detection, and Repair: Kirtland Air Force Base Leak Detection and Repair Program  

NLE Websites -- All DOE Office Websites (Extended Search)

Best Management Practice Best Management Practice Case Study #3 Distribution System Audits, Leak Detection, and Repair Kirtland Air Force Base - Leak Detection and Repair Program Overview Kirtland Air Force Base (AFB) performed an award winning leak detection and repair program in 2006. The results of the project are saving Kirtland AFB 179 million gallons each year, which is over 16% of the total water use at the base. Kirtland AFB is located on 52,000 acres, southeast and adjacent to Albuquerque, New Mexico. The area is a high altitude desert, only receiving about 8 inches of rain each year. Kirtland AFB draws water from an under- ground aquifer via seven production wells through- out the base. The base also has access to water from the City of Albuquerque. The underground water

192

Rapid pipeline repair technology for war damage recovery. Technical note  

SciTech Connect

This report documents the development of three experimental pipeline couplers for rapid repair of fuel lines damaged in an attack. The experimental couplers are: (1) the Cold Forge coupler, (2) the Internal Coupler, and (3) the Inflatable Seal Coupler. The focus of the evaluation was to determine the feasibility of rapidly repairing bomb-damaged fuel lines with each coupler, particularly underground pipelines made of carbon steel. Evaluating the feasibility of repair with each coupler was based on such aspects as installations speed and effectiveness. The test results confirmed that each coupler could be used during base recovery, operations to rapidly and effectively repair a fuel pipeline that may be out-of-round or highly misaligned. Recommended that each experimental coupler be taken into advanced development for extensive testing and field evaluation. Base recovery, Expedient pipeline repair, Utility repair.

Anguiano, G.

1993-06-01T23:59:59.000Z

193

Housing and Dining Services Spring Break Information March 2011  

E-Print Network (OSTI)

charge to stay over spring break. Kitchenettes will be open in all residence halls over the break period equipment · Refrigerators with food may be left on · Close and lock your windows and doors · Turn off your

194

Winter Break 2011 (January) Knowledge is Power Program  

E-Print Network (OSTI)

(Wellington, Colorado) Winter Break 2009 (January) � La Union del Pueblo Entero (San Juan, TX) (10 participants) � Community Collaborations, Flood Relief (Atlanta, GA) (10 participants) Weekend Breaks Fall 2009

195

Spectral Energy Dissipation due to Surface Wave Breaking  

Science Journals Connector (OSTI)

A semiempirical determination of the spectral dependence of the energy dissipation due to surface wave breaking is presented and then used to propose a model for the spectral dependence of the breaking strength parameter b, defined in the O. M. ...

Leonel Romero; W. Kendall Melville; Jessica M. Kleiss

2012-09-01T23:59:59.000Z

196

Cells Forming Blood Vessels Send Their Copper to the Edge | Advanced Photon  

NLE Websites -- All DOE Office Websites (Extended Search)

A Molecular Cause for One Form of Deafness A Molecular Cause for One Form of Deafness Water Theory is Watertight Nanowire Micronetworks from Carbon-Black Nanoparticles A Key Step in Repairing DNA Double-Strand Breaks An X-ray Rainbow Science Highlights Archives: 2013 | 2012 | 2011 | 2010 2009 | 2008 | 2007 | 2006 2005 | 2004 | 2003 | 2002 2001 | 2000 | 1998 | Subscribe to APS Science Highlights rss feed Cells Forming Blood Vessels Send Their Copper to the Edge FEBRUARY 20, 2007 Bookmark and Share Areas at the tips of HMVEC filopodia extensions were scanned by XFM at high resolution. The optical image is shown to the right and metal maps are shown to the left. False color images of P, Cu, and Zn are shown in the red, green and blue images respectively, and their overlay is shown to the lower right, demonstrating a transfer of cellular copper across the cell

197

Low Dose Radiation Research Program: Howard L. Liber  

NLE Websites -- All DOE Office Websites (Extended Search)

Howard L. Liber Howard L. Liber Department of Environmental and Radiological Health Sciences Colorado State University Currently Funded Projects Molecular Mechanisms of Radiation-Induced Genomic Instability in Human Cells Technical Abstracts 2003 Workshop: Delayed genomic instability in human lymphoblasts exposed to 137Cs y-rays radiation Schwartz, J.L., Jordan, R., Lenarczyk, M. and Liber, H.L. 2002 Workshop: Molecular Mechanisms of Radiation-Induced Genomic Instability in Human Cells. Liber, H.L. and Schwartz, J.L. Publications Zhang, Y., Zhou, J., Held, K.D., Redmond, R.W., Prise, K.M., and Liber, H.L. (2008). Deficiencies of double-strand break repair factors and effects on mutagenesis in directly [gamma]-irradiated and medium-mediated bystander human lymphoblastoid cells. Radiation Research 169(2):197-206.

198

A Molecular Cause for One Form of Deafness | Advanced Photon Source  

NLE Websites -- All DOE Office Websites (Extended Search)

Water Theory is Watertight Water Theory is Watertight Nanowire Micronetworks from Carbon-Black Nanoparticles A Key Step in Repairing DNA Double-Strand Breaks An X-ray Rainbow An Insulating Breakthrough Science Highlights Archives: 2013 | 2012 | 2011 | 2010 2009 | 2008 | 2007 | 2006 2005 | 2004 | 2003 | 2002 2001 | 2000 | 1998 | Subscribe to APS Science Highlights rss feed A Molecular Cause for One Form of Deafness FEBRUARY 12, 2007 Bookmark and Share Gerard Wong, a professor of materials science and engineering, of physics, and of bioengineering at Illinois, and colleagues have found an underlying molecular cause for one form of deafness, while exploring the physics of hearing. (Photo by L. Brian Stauffer) Scientists exploring the physics of hearing have found an underlying molecular cause for one form of deafness, and a conceptual connection

199

10089_Primer_v1i2_spreads  

NLE Websites -- All DOE Office Websites (Extended Search)

5 Vol. 2, 5 Vol. 2, Issue 1 8 / THE PRIMER CONTACT THE PRIMER David Gilbert, Editor gilbert21@llnl.gov (925) 296-5643 New Names/Transitions Eric Abbott contract Laboratory Technician Ranjana Ambannavar contract Laboratory Technician Mariana Anaya Biomedical Scientist Shane Brubaker Computer Scientist Sandra Chaparro Career appointment Jarrod Chapman Post Doc appointment Vanessa Chiu Scientific Technician Peg Folta Acting Department Head for Informatics Navjett Kaur Laboratory Technician Victor Kunin Post Doc Konstantinos Mavrommatis Post Doc Donna McCown contract Secretary Brenda Mitchell contract Secretary Hope Tice Career appointment Jennifer White Sequencing Supervisor Matthew Zane Scientific Technician JAN 14 Stephen West, Cancer Research UK "Double-strand Break Repair in Mammalian Cells"

200

Laboratory Studies of Nonlinear and Breaking Surface Waves  

E-Print Network (OSTI)

A. Breaking-wave generation . . . . . . . . . . . . . . . .of the wave tank. . . . . . . . . . . . . . . . . . . .On steep gravity waves meeting a vertical wall: a triple

Drazen, David

2006-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "double-strand break repair" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


201

Breaking the Fuel Cell Cost Barrier  

NLE Websites -- All DOE Office Websites (Extended Search)

Breaking the Fuel Cell Cost Barrier Breaking the Fuel Cell Cost Barrier AMFC Workshop May 8 th , 2011, Arlington, VA Shimshon Gottesfeld, CTO The Fuel Cell Cost Challenge 2 CellEra's goal - achieve price parity with incumbents earlier on in market entry process ! Mainstream Polymer Electrolyte Fuel Cell ( PEM) Cost Barriers 3 Graphite / stainless steel hardware Acidic membrane Platinum based electrodes Cost barriers deeply embedded in core tech materials BOM-based cost barriers - 90% of stack cost Cost volatility - Platinum $500/Oz - $2,500/Oz The possibility of an OH - ion conducting membrane 4 Non-acidic membrane CellEra Took Advantage of this Opportunity A new type of membrane component with potential for strong fuel cell cost cuts was revealed in 2006, but was accompanied by general industry skepticism

202

Breaking the Code of Cell Communication  

NLE Websites -- All DOE Office Websites (Extended Search)

Breaking the Code of Cell Communication Breaking the Code of Cell Communication A method that cells use to communicate and coordinate activities has been confirmed by researchers from Cornell University, Monsanto Co., and Argonne. This work could lead to new drugs for the fight against such diseases as cystic fibrosis and the bubonic plague, or to new technologies that perform useful environmental tasks such as filtering water. The structure of the key protein that enables quorum-sensing bacteria to communicate and spread infection. Above: The structure of the key protein that enables quorum-sensing bacteria to communicate and spread infection. Biologists have theorized that bacteria communicate by releasing and sensing chemical pheromones to detect their population densities, an activity called "quorum sensing." This theory was confirmed by

203

Broadband acoustic imaging of breaking waves  

Science Journals Connector (OSTI)

An acoustic array was deployed in the near?surface layer in Saanich Inlet BC to image breaking waves using only the naturally occurring acoustical radiation in the band (160 and 2000 Hz) from the breaking region. The 15?element array was configured as a horizontal cross with an 8?m aperture bottom?moored and positioned nominally 3 m beneath the surface. A novel broadband scheme combined information at six independent frequencies above about 400 Hz to yield unambiguous resolved images. A parametric image analysis shows that the images align closely with the wind and can be observed moving downwind with a speed roughly equal to the dominant phase speed of the wind waves. Absolute power levels are found to be consistent with previously published results. The data also provide inferences regarding the sound generation mechanism at ‘‘collective oscillation’’ frequencies below about 400 Hz. [Work supported by ONR.

Rex K. Andrew; David M. Farmer; R. Lynn Kirlin

1997-01-01T23:59:59.000Z

204

External breaking of ground-state symmetry  

Science Journals Connector (OSTI)

Ground-state symmetry can be broken by an external field. The threshold value of the symmetry-breaking field may be roughly estimated by comparing experimentally the behavior of two identical physical quantities, as functions of the field, for the set of field directions equivalent, with respect to the action, to a group generator. The kinetic coefficients as the functions of magnetic induction B are used as an illustration.

S. Malinowski

1994-01-01T23:59:59.000Z

205

Dec. 13-Jan. 3 Winter Break  

E-Print Network (OSTI)

Dec. 13-Jan. 3 Winter Break Students must be out of residence halls by 10 a.m. on Dec. 13. Students may return to residence halls after 1 p.m. on Jan. 3. Jan. 8 Last day to drop a course without a fee Dance Company Grupo de Rua 8 p.m., Campbell Hall Arts & Lectures tickets may be purchased by calling

California at Santa Barbara, University of

206

Charge breaking bounds in the Zee model  

E-Print Network (OSTI)

We study the possibility that charge breaking minima occur in the Zee model. We reach very different conclusions from those attained in simpler, two Higgs doublet models, and the reason for this is traced back to the existence of cubic terms in the potential. A scan of the Zee model's parameter space shows that CB is restricted to a narrow region of values of the parameters.

A. Barroso; P. M. Ferreira

2005-10-11T23:59:59.000Z

207

Window Replacement, Rehabilitation, & Repair Guides- Building America Top Innovation  

Energy.gov (U.S. Department of Energy (DOE))

Building America team Building Science Corporation guides contractors through several options for repairing or replacing old windows to improve air sealing and thermal performance.

208

Underwater Pipeline Repair in Difficult Seabed Conditions: Problems and Solutions  

Science Journals Connector (OSTI)

This paper examines the application of innovative technology to significantly reduce the time for a major repair to a 36 inch diameter buried submarine pipeline.

B. A. Jones; N. Wright

1994-01-01T23:59:59.000Z

209

Modelling repairable system reliability with explanatory variables and repair and maintenance actions  

Science Journals Connector (OSTI)

......However, in general, in RMs...overhaul of an engine (a midlife...used with diesel engines...g. from diesel engines, such as...is a quite general model, and...analysis of diesel engine failure data...systems with general repair......

Diederik Lugtigheid; agan Banjevic; Andrew K. S. Jardine

2004-04-01T23:59:59.000Z

210

Flavopiridol Potentiates the Cytotoxic Effects of Radiation in Radioresistant Tumor Cells in Which p53 is Mutated or Bcl-2 is Overexpressed  

SciTech Connect

Purpose: Loss of the cell-cycle regulatory protein p53 or overexpression of the antiapoptotic protein Bcl-2 is associated with resistance to radiation in several types of cancer cells. Flavopiridol, a synthetic flavone, inhibits the growth of malignant tumors cells in vitro and in vivo through multiple mechanisms. The purpose of the present study is to clarify whether flavopiridol enhances the cytotoxic effects of radiation in tumor cells that contain dysfunction p53 or that overexpress Bcl-2. Methods and Materials: A human glioma cell line (A172/mp53) stably transfected with a plasmid containing mutated p53 and a human cervical cancer cell line (HeLa/bcl-2) transfected with a bcl-2 expression plasmid were used. Cells were incubated with flavopiridol for 24 h after radiation, and then cell viability was determined by a colony formation assay. Foci of phosphorylated histone H2AX were also evaluated as a sensitive indicator of DNA double-strand breaks. Results: Compared with the parental wild-type cells, both transfected cell lines were more resistant to radiation. Post-treatment with flavopiridol increased the cytotoxic effects of radiation in both transfected cell lines, but not in their parental wild-type cell lines. Post-treatment with flavopiridol inhibited sublethal damage repair as well as the repair of DNA double-strand breaks in response to radiation. Conclusions: Flavopiridol enhanced the cytotoxic effect of radiation in radioresistant tumor cells that harbor p53 dysfunction or Bcl-2 overexpression. A combination treatment of flavopiridol with radiation has the potential to conquer the radioresistance of malignant tumors induced by the genetic alteration of p53 or bcl-2.

Hara, Takamitsu [Department of Radiology, Yokohama City University Graduate School of Medicine, Yokohama (Japan); Omura-Minamisawa, Motoko [Department of Radiology, Yokohama City University Graduate School of Medicine, Yokohama (Japan)], E-mail: momuram@med.yokohama-cu.ac.jp; Kang, Yun; Cheng, Chao; Inoue, Tomio [Department of Radiology, Yokohama City University Graduate School of Medicine, Yokohama (Japan)

2008-08-01T23:59:59.000Z

211

Substance-specific and shared transcription and epigenetic changes in the human hippocampus chronically exposed to cocaine and alcohol  

Science Journals Connector (OSTI)

...finger, CCCH-type with G patch domain 0.55 9.2E-05 0...the end of incubation for end repair. Synthesized double-stranded...the Klenow fragment for end repair. The 5' end of the DNA fragments...the Illumina Genome Analyzer Pipeline (GApipeline) and aligned...

Zhifeng Zhou; Qiaoping Yuan; Deborah C. Mash; David Goldman

2011-01-01T23:59:59.000Z

212

Availability Analysis of Repairable Computer Systems and Stationarity Detection  

E-Print Network (OSTI)

Availability Analysis of Repairable Computer Systems and Stationarity Detection Bruno Sericola computer system and we assume, as usual, that the system is modeled by a finite Markov process. We propose of performability measures. Index TermsÐRepairable computer systems, dependability, availability, performability

Sericola, Bruno

213

Proceedings of design, repair, and refurbishment of steam turbines  

SciTech Connect

This book reports on the proceedings of design, repair and refurbishment of steam engines. Topics covered include: Advisor/Expert Systems for Steam Turbines; Moisture Effects on the Operating and Performance of Steam Turbines; Turbine Steam Path Development; Repair and Refurbishment of the Electric Generator Components; and Advanced Steam Turbine Designs.

Warnock, A.S. (Lehigh Univ., PA (United States))

1991-01-01T23:59:59.000Z

214

Improved Criteria for the Repair of Fabrication Flaws  

SciTech Connect

Section III of the American Society of Mechanical Engineers (ASME) Boiler and Pressure Vessel Code for nuclear power plant components requires radiographic examinations (RT) of welds and requires repairs for RT indications that exceed code acceptable sizes. This paper describes research that has generated data on welding flaws, which indicated that the largest flaws occur in repaired welds. The fabrication flaws were detected in material removed from cancelled nuclear power plants using high sensitivity Nondestructive Examination (NDE) and validated by complementary NDE and destructive testing. Evidence suggests that repairs are often for small and benign RT indications at locations buried within the vessel or pipe wall. Probabilistic fracture mechanics calculations are described in this paper to predict the increases in vessel and piping failure probabilities caused by the repair-induced flaws. Calculations address failures of embrittled vessel welds for pressurized thermal shock (PTS) transients and piping failures caused by fatigue crack growth. For vessels the small flaws, which are relatively common, can cause brittle fracture, such that the rarely encountered repair flaws of large sizes gave only modestly increased failure probabilities. Calculations for piping show that only relatively large fabrication flaws can cause failures because of the ductile nature of the piping material. The large repair flaws therefore significantly increased the failure probabilities. The paper recommends the use of more discriminating ultrasonic examinations in place of RT examinations along with repair criteria based on a fitness-for-purpose approach that minimize the number of unjustified repairs.

Doctor, Steven R.; Schuster, George J.; Simonen, Fredric A.

2004-12-01T23:59:59.000Z

215

Nucleotide Excision Repair Pathway Genes and Oral Premalignant Lesions  

Science Journals Connector (OSTI)

...repair protein XPA binds replication protein A (RPA). J Biol Chem 1995;270:4152-7. 18 van Gool AJ, Citterio E, Rademakers S, et al. The Cockayne syndrome B protein, involved in transcription-coupled DNA repair, resides in an RNA polymerase...

Yunfei Wang; Margaret R. Spitz; J. Jack Lee; Maosheng Huang; Scott M. Lippman; Xifeng Wu

2007-06-15T23:59:59.000Z

216

The Cable Repair Ship H.M.T.S. Monarch  

Science Journals Connector (OSTI)

... -6), members of the general public had an opportunity of inspecting the Post Office cable repair ship Monarch, which was lying in the Thames off the Tower. This ship ... fitted out with the special gear necessary for carrying out all the operations required in cable laying and repair, on which service she is at sea for most of the ...

1934-10-06T23:59:59.000Z

217

Spontaneous Supersymmetry Breaking Induced by Vacuum Condensates  

E-Print Network (OSTI)

We propose a novel mechanism of spontaneous supersymmetry breaking which relies upon an ubiquitous feature of Quantum Field Theory, vacuum condensates. Such condensates play a crucial r\\^{o}le in many phenomena. Examples include Unruh effect, superconductors, particle mixing, and quantum dissipative systems. We argue that in all these phenomena supersymmetry, when present, is spontaneously broken. Evidence for our conjecture is given for the Wess--Zumino model, that can be considered an approximation to the supersymmetric extensions of the above mentioned systems. The magnitude of the effect is estimated for a recently proposed experimental setup based on an optical lattice.

Antonio Capolupo; Marco Di Mauro

2012-08-29T23:59:59.000Z

218

Chip breaking system for automated machine tool  

DOE Patents (OSTI)

The invention is a rotary selectively directional valve assembly for use in an automated turret lathe for directing a stream of high pressure liquid machining coolant to the interface of a machine tool and workpiece for breaking up ribbon-shaped chips during the formation thereof so as to inhibit scratching or other marring of the machined surfaces by these ribbon-shaped chips. The valve assembly is provided by a manifold arrangement having a plurality of circumferentially spaced apart ports each coupled to a machine tool. The manifold is rotatable with the turret when the turret is positioned for alignment of a machine tool in a machining relationship with the workpiece. The manifold is connected to a non-rotational header having a single passageway therethrough which conveys the high pressure coolant to only the port in the manifold which is in registry with the tool disposed in a working relationship with the workpiece. To position the machine tools the turret is rotated and one of the tools is placed in a material-removing relationship of the workpiece. The passageway in the header and one of the ports in the manifold arrangement are then automatically aligned to supply the machining coolant to the machine tool workpiece interface for breaking up of the chips as well as cooling the tool and workpiece during the machining operation.

Arehart, Theodore A. (Clinton, TN); Carey, Donald O. (Oak Ridge, TN)

1987-01-01T23:59:59.000Z

219

Acoustic radiation due to surface wave breaking.  

Science Journals Connector (OSTI)

While wave breaking is continually occurring at the sea surface its transient and sporadic nature makes it difficult to measure. Experimental results are presented that show how acoustic methods can be used as a remote sensor of this fundamental process. Sea surface?generated acoustic radiation (40 to 4000 Hz) is directly related to a quantitative measure of the boundary dynamics; i.e. the Toba variable. The frequency spectrum of the radiation remains remarkably unchanged over a wide range of environmental conditions but the correlation between the sound pressure level and the Toba variable undergoes an abrupt change when spilling breakers start to occur. Results support the use of acoustics to remotely measure the rate of energy being dissipated by wave breaking and the wavelength of the dominant gravity wave component. Theoretical studies have related the field measurements to analytical and laboratory results cited in the literature indicating that remote monitoring of the rate of occurrence and size distribution of ‘‘infant’’ (freshly entrained) bubbles may be possible if splashes on the surface do not radiate significant sound. Signal processing algorithms for the remote measurements discussed above are enhanced by eigenstructure analysis of the measured cross?spectral density matrix. [Work sponsored by ONR and NUSC.

Robert M. Kennedy; Stewart A. L. Glegg

1992-01-01T23:59:59.000Z

220

Exploring Cartan gravity with dynamical symmetry breaking  

E-Print Network (OSTI)

It has been known for some time that General Relativity can be regarded as a Yang-Mills-type gauge theory in a symmetry broken phase. In this picture the gravity sector is described by an $SO(1,4)$ or $SO(2,3)$ gauge field $A^{a}_{\\phantom{a}b\\mu}$ and Higgs field $V^{a}$ which acts to break the symmetry down to that of the Lorentz group $SO(1,3)$. This symmetry breaking mirrors that of electroweak theory. However, a notable difference is that while the Higgs field $\\Phi$ of electroweak theory is taken as a genuine dynamical field satisfying a Klein-Gordon equation, the gauge independent norm $V^2\\equiv \\eta_{ab}V^{a}V^{b}$ of the Higgs-type field $V^a$ is typically regarded as non-dynamical. Instead, in many treatments $V^a$ does not appear explicitly in the formalism or is required to satisfy $V^2 = \\mathrm{const.} \

H. F. Westman; T. G. Zlosnik

2014-03-24T23:59:59.000Z

Note: This page contains sample records for the topic "double-strand break repair" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


221

DNA damage and repair in human skin in situ  

SciTech Connect

Understanding the molecular and cellular origins of sunlight-induced skin cancers in man requires knowledge of the damages inflicted on human skin during sunlight exposure, as well as the ability of cells in skin to repair or circumvent such damage. Although repair has been studied extensively in procaryotic and eucaryotic cells - including human cells in culture - there are important differences between repair by human skin cells in culture and human skin in situ: quantitative differences in rates of repair, as well as qualitative differences, including the presence or absence of repair mechanisms. Quantitation of DNA damage and repair in human skin required the development of new approaches for measuring damage at low levels in nanogram quantities of non-radioactive DNA. The method allows for analysis of multiple samples and the resulting data should be related to behavior of the DNA molecules by analytic expressions. Furthermore, it should be possible to assay a variety of lesions using the same methodology. The development of new analysis methods, new technology, and new biochemical probes for the study of DNA damage and repair are described. 28 refs., 4 figs.

Sutherland, B.M.; Gange, R.W.; Freeman, S.E.; Sutherland, J.C.

1987-01-01T23:59:59.000Z

222

Española entrepreneur breaks ground; expansion will create 50...  

NLE Websites -- All DOE Office Websites (Extended Search)

Espaola entrepreneur breaks ground; expansion will create 50 new jobs Community Connections: Your link to news and opportunities from Los Alamos National Laboratory Latest Issue:...

223

Symmetry Breaking in Graphene Gregory S. Boebinger, National...  

NLE Websites -- All DOE Office Websites (Extended Search)

Symmetry Breaking in Graphene Gregory S. Boebinger, National High Magnetic Field Laboratory DMR-Award 0654118 DC Field Facility While the laws of physics are often symmetric,...

224

Dynamical electroweak symmetry breaking and the top quark  

SciTech Connect

In this talk, I discuss theories of dynamical electroweak symmetry breaking, with emphasis on the implications of a heavy top quark on the weak interaction {rho} parameter.

Chivukula, R.S. [Boston Univ., MA (United States)

1997-01-01T23:59:59.000Z

225

LANL breaks ground on key sediment control project  

NLE Websites -- All DOE Office Websites (Extended Search)

Sediment control project LANL breaks ground on key sediment control project Called "grade-control" structures, the approximately 2 million features are up to eight feet high and...

226

Breaking Through the Glass Ceiling: Recent Experimental Approaches...  

NLE Websites -- All DOE Office Websites (Extended Search)

Through the Glass Ceiling: Recent Experimental Approaches to Probe the Properties of Supercooled Liquids near the Glass Breaking Through the Glass Ceiling: Recent Experimental...

227

Breaking through the Glass Ceiling: The Correlation Between the...  

NLE Websites -- All DOE Office Websites (Extended Search)

through the Glass Ceiling: The Correlation Between the Self-Diffusivity in and Krypton Permeation through Deeply Breaking through the Glass Ceiling: The Correlation Between the...

228

Tips for Running an Air Conditioner Without Breaking the Bank  

Energy.gov (U.S. Department of Energy (DOE))

With summer temperatures rising, air conditioners are working overtime, but it is possible to enjoy to cool comfort without breaking the bank.

229

Analysis of a carbon composite overwrap pipeline repair system  

Science Journals Connector (OSTI)

A relatively new method has been developed to stop external corrosion and structurally reinforce steel pipes by external wrapping of damaged sections using fibre reinforced polymer (FRP) materials. Several different defect geometries representing corrosion patches on steel pipe were characterized using finite-element analysis, by changing the circumferential length of the defect. Pipe vessels containing these defects along with the composite structural repairs were modeled and the results were compared to field tests to determine the effectiveness of the repairs. It was found that the defect width around the circumference had little impact on the ultimate rupture pressure of the repaired vessel, but influenced the stress state in the underlying pipe substrate.

J.M. Duell; J.M. Wilson; M.R. Kessler

2008-01-01T23:59:59.000Z

230

Repair-Mediated Duplication by Capture of Proximal Chromosomal DNA Has Shaped Vertebrate Genome  

E-Print Network (OSTI)

computational pipeline that takes advantage of the repetitive structure of genomes to detect repairRepair-Mediated Duplication by Capture of Proximal Chromosomal DNA Has Shaped Vertebrate Genome if not repaired promptly. Experimental systems have shown that DSB repair in eukaryotic cells is often imperfect

Feschotte, Cedric

231

A tissue engineering strategy for integrative cartilage repair  

E-Print Network (OSTI)

Tissue engineering for cartilage repair is a promising approach for improving the healing of articular defects, as biomaterials and growth factors can be supplied directly to a focal lesion. However, integrating neo-tissue ...

Mroszczyk, Keri A

2014-01-01T23:59:59.000Z

232

Repair of Damaged DNA by Arabidopsis Cell Extract  

Science Journals Connector (OSTI)

...and irradiated at the doses indicated in figure legends...the repair of a model ionizing radiation-induced DNA strand...their genomes from a wide range of genotoxic stresses...Arabidopsis cytology genetics radiation effects Cell Extracts...

Anatoliy Li; David Schuermann; Francesca Gallego; Igor Kovalchuk; Bruno Tinland

233

How Do Bacteria Repair Damage from the Sun? | Advanced Photon...  

NLE Websites -- All DOE Office Websites (Extended Search)

| Subscribe to APS Science Highlights rss feed How Do Bacteria Repair Damage from the Sun? JANUARY 22, 2014 Bookmark and Share Modeling of UvrAUvrB SAXS data. Panel A shows the...

234

Automated Image Processing for the Analysis of DNA Repair Dynamics  

E-Print Network (OSTI)

The efficient repair of cellular DNA is essential for the maintenance and inheritance of genomic information. In order to cope with the high frequency of spontaneous and induced DNA damage, a multitude of repair mechanisms have evolved. These are enabled by a wide range of protein factors specifically recognizing different types of lesions and finally restoring the normal DNA sequence. This work focuses on the repair factor XPC (xeroderma pigmentosum complementation group C), which identifies bulky DNA lesions and initiates their removal via the nucleotide excision repair pathway. The binding of XPC to damaged DNA can be visualized in living cells by following the accumulation of a fluorescent XPC fusion at lesions induced by laser microirradiation in a fluorescence microscope. In this work, an automated image processing pipeline is presented which allows to identify and quantify the accumulation reaction without any user interaction. The image processing pipeline comprises a preprocessing stage where the ima...

Riess, Thorsten; Tomas, Martin; Ferrando-May, Elisa; Merhof, Dorit

2011-01-01T23:59:59.000Z

235

Solar Water Heating System Maintenance and Repair | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Water Heating System Maintenance and Repair Water Heating System Maintenance and Repair Solar Water Heating System Maintenance and Repair May 30, 2012 - 2:35pm Addthis Rooftop solar water heaters need regular maintenance to operate at peak efficiency. | Photo from iStockphoto.com Rooftop solar water heaters need regular maintenance to operate at peak efficiency. | Photo from iStockphoto.com Solar energy systems require periodic inspections and routine maintenance to keep them operating efficiently. Also, from time to time, components may need repair or replacement. You should also take steps to prevent scaling, corrosion, and freezing. You might be able to handle some of the inspections and maintenance tasks on your own, but others may require a qualified technician. Ask for a cost estimate in writing before having any work done. For some systems, it may

236

Coherent testing and simulation of composite blade repair  

E-Print Network (OSTI)

COHERENT TESTING AND SIMULATION OF COMPOSITE BLADE REPAIR A Thesis by BRIAN GLENN MCNICHOLS Submitted to the Office of Graduate Studies of Texas A&M University in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE... December 1997 Major Subject: Mechanical Engineering COHERENT TESTING AND SIMULATION OF COMPOSITE BLADE REPAIR A Thesis by BRIAN GLENN MCNICHOLS Submitted to Texas A&M University In partial fulfillment of the requirements for the degree of MASTER...

McNichols, Brian Glenn

2012-06-07T23:59:59.000Z

237

Resonant Instability in Mountain Waves: Breaking at Subcritical Mountain Heights  

E-Print Network (OSTI)

Resonant Instability in Mountain Waves: Breaking at Subcritical Mountain Heights Kevin Viner1 and breaks subcritical critical Nh/U = 0.5 Nh/U = 0.8 #12;Subcritical Instability: An Example three peaks · Nh/U = 0.6 · U/NL = 0.1 · nonrotating · Time-dependent model initialized with subcritical steady wave

238

Manual HVOF thermal spray repair of nickel aluminum bronze castings  

SciTech Connect

Manual high velocity oxyfuel (HVOF) thermal spray repairs were accomplished on a large nickel aluminum bronze propeller castings. The repairs were done on three different configurations of surface defects of up to 100 square inches and as deep as 90 mils. Nickel aluminum bronze alloy powder, sieve sized for the HVOF process, was sprayed. High quality, high coating density, repairs were achieved even on porous areas of the castings. Prior to performance of the repairs, a procedure was qualified in accordance with MIL-STD-1687 and a mock-up simulating the repair was produced. After HVOF spraying of the mock-up, the sprayed surface was sanded, milled, and drilled to determine how finishing of the actual castings would be done. After successful procedure qualification, the HVOF equipment was moved to the job site, metal masking was devised for the spray areas and grit blasting and manual HVOF spraying was done. Results of HVOF coating chemical analyses, bend tests, coating tensile bond strength tests, coating microscopic examinations, and mock-up evaluations are reported along with the spray procedures and techniques used in the repairs.

Brenna, R.T.; McCaw, R.L.; Pugh, J.L.

1994-12-31T23:59:59.000Z

239

Deepwater pipeline-repair system deployed to Mediterranean  

SciTech Connect

The latest phase in development of a deepwater pipeline-repair system received full-scale trials earlier this summer in Norway and has been deployed on standby for the Trans-Mediterranean pipeline by operator SNAM. In Stavanger harbor in June, Sonsub International Inc.`s Arcos diverless repair system underwent successful shallow-water trials that employed all the system`s equipment. (Arcos is an Italian acronym for attrezzaturre per la riparazione di condotte sottomarine-subsea pipe repair tooling.) The system is the most recent development in an evolution of efforts to develop a diverless pipeline-repair system for deepwater use. The prototype PRS (pipeline repair system) received deepwater (300m) trials offshore southern Italy in 1992. It used two work-class ROVs. In 1995, a modified PRS, renamed the DSRS (diverless sealine repair system), underwent shallow-water trials, also offshore southern Italy, that led to a modification of its pipe-lifting system. In 1997, the DSRS underwent more shallow-water trials, this time in Stavanger, which led to improvement in the spool-installation module. According to Sonsub, this refined version of the Arcos employs a low-force modular concept that is ROV supported and can be adapted quickly and easily to a wide range of pipe sizes.

True, W.R.

1998-11-16T23:59:59.000Z

240

Breaking wind waves as a source of ambient noise  

Science Journals Connector (OSTI)

A theoretical model for the prediction of ambient noise level due to collective oscillations of air bubbles under breaking wind waves is presented. The model uses a budget of the energy flux from the breaking waves to quantify acoustic power radiation by a bubble cloud. A shift of the noise spectra to lower frequency due to collective bubble oscillation is assumed. The model derives good estimates of the magnitude slope and frequency range of the noise spectra using the wind speed or height of breaking waves.

Pavlo Tkalich; Eng Soon Chan

2002-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "double-strand break repair" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


241

RG analysis of magnetic catalysis in dynamical symmetry breaking  

SciTech Connect

We perform the renormalization group analysis on the dynamical symmetry breaking under strong external magnetic field, studied recently by Gusynin, Miransky and Shovkovy. We find that any attractive four-Fermi interaction becomes strong in the low energy, thus leading to dynamical symmetry breaking. When the four-Fermi interaction is absent, the {beta}-function for the electromagnetic coupling vanishes in the leading order in 1/N. By solving the Schwinger-Dyson equation for the fermion propagator, we show that in 1/N expansion, for any electromagnetic coupling, dynamical symmetry breaking occurs due to the presence of Landau energy gap by the external magnetic field. 5 refs.

Hong, Deog Ki [Pusan National Univ., (Korea). Dept. of Physics]|[Florida Univ., Gainesville, FL (United States); Kim, Youngman [Hanyang Univ., Seoul (Korea). Dept. of Physics

1996-05-01T23:59:59.000Z

242

Single molecule techniques in DNA repair: A primer  

Science Journals Connector (OSTI)

Abstract A powerful new approach has become much more widespread and offers insights into aspects of DNA repair unattainable with billions of molecules. Single molecule techniques can be used to image, manipulate or characterize the action of a single repair protein on a single strand of DNA. This allows search mechanisms to be probed, and the effects of force to be understood. These physical aspects can dominate a biochemical reaction, where at the ensemble level their nuances are obscured. In this paper we discuss some of the many technical advances that permit study at the single molecule level. We focus on DNA repair to which these techniques are actively being applied. DNA repair is also a process that encompasses so much of what single molecule studies benefit – searching for targets, complex formation, sequential biochemical reactions and substrate hand-off to name just a few. We discuss how single molecule biophysics is poised to transform our understanding of biological systems, in particular DNA repair.

Craig D. Hughes; Michelle Simons; Cassidy E. Mackenzie; Bennett Van Houten; Neil M. Kad

2014-01-01T23:59:59.000Z

243

Symmetry Breaking of H2 Dissociation by a Single Photon  

NLE Websites -- All DOE Office Websites (Extended Search)

Symmetry Breaking of H2 Symmetry Breaking of H2 Dissociation by a Single Photon Symmetry Breaking of H2 Dissociation by a Single Photon Print Wednesday, 25 July 2007 00:00 A single hydrogen (or deuterium) molecule consists of only two protons (deuterons) and two electrons and is perfectly symmetric. Linearly polarized photons are similarly symmetric. So one might think that the angular distribution of photoelectrons resulting from photoionization of the molecule by the photon accompanied by dissociation into a hydrogen atom and a hydrogen ion would itself be symmetric. However, an international team of researchers from Germany, Spain, and the U.S. has now shown that this need not be the case. When there are multiple quantum paths for the process, interference between waves in the coherent superposition of electron states (which exists when the molecular fragments are still close together) skews the distribution by breaking the molecular symmetry.

244

Pantex breaks ground on renewable energy project | National Nuclear  

NLE Websites -- All DOE Office Websites (Extended Search)

breaks ground on renewable energy project | National Nuclear breaks ground on renewable energy project | National Nuclear Security Administration Our Mission Managing the Stockpile Preventing Proliferation Powering the Nuclear Navy Emergency Response Recapitalizing Our Infrastructure Continuing Management Reform Countering Nuclear Terrorism About Us Our Programs Our History Who We Are Our Leadership Our Locations Budget Our Operations Media Room Congressional Testimony Fact Sheets Newsletters Press Releases Speeches Events Social Media Video Gallery Photo Gallery NNSA Archive Federal Employment Apply for Our Jobs Our Jobs Working at NNSA Blog Home > NNSA Blog > Pantex breaks ground on renewable energy project Pantex breaks ground on renewable energy project Posted By Office of Public Affairs Today was a rare windless day on the plains of the Texas Panhandle, but

245

Breaking Ground in Miami-Dade | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Breaking Ground in Miami-Dade Breaking Ground in Miami-Dade Breaking Ground in Miami-Dade October 15, 2010 - 4:28pm Addthis Existing Miami-Dade county water treatment facility. Existing Miami-Dade county water treatment facility. Andy Oare Andy Oare Former New Media Strategist, Office of Public Affairs Officials from Miami-Dade County and the U.S. Department of Energy were on hand Wednesday, October 13th to formally break ground on an innovative project that will help improve the energy efficiency of one of the county's major water treatment facilities. The project will upgrade and expand the existing power generation system at the water plant which generates electricity from digester gas produced at the plant. Landfill gas, which is produced from the Solid Waste Department's South Dade Landfill, will be collected and piped across a

246

Pantex breaks ground on renewable energy project | National Nuclear  

National Nuclear Security Administration (NNSA)

breaks ground on renewable energy project | National Nuclear breaks ground on renewable energy project | National Nuclear Security Administration Our Mission Managing the Stockpile Preventing Proliferation Powering the Nuclear Navy Emergency Response Recapitalizing Our Infrastructure Continuing Management Reform Countering Nuclear Terrorism About Us Our Programs Our History Who We Are Our Leadership Our Locations Budget Our Operations Media Room Congressional Testimony Fact Sheets Newsletters Press Releases Speeches Events Social Media Video Gallery Photo Gallery NNSA Archive Federal Employment Apply for Our Jobs Our Jobs Working at NNSA Blog Home > NNSA Blog > Pantex breaks ground on renewable energy project Pantex breaks ground on renewable energy project Posted By Office of Public Affairs Today was a rare windless day on the plains of the Texas Panhandle, but

247

Vermont Yankee simulator qualification: large-break LOCA  

SciTech Connect

Yankee Atomic Electric Company (YAEC) has developed simulator benchmark capabilities for the Seabrook, Maine Yankee, and Vermont Yankee Nuclear Power Station (VYNPS) simulators. The goal is to establish that each simulator has a satisfactory real-time response for different scenarios that will enhance operator training. Vermont Yankee purchased a full-scope plane simulator for the VYNPS, a four-unit boiling water reactor with a Mark-I containment. The following seven benchmark cases were selected by YAEC and VYNPC to supplement the Simulator Acceptance Test Program: (1) control rod swap; (2) partial reactor scram; (3) recirculation pump trip; (4) main steam isolation valve (MSIV) closure without scram, (5) main steamline break, (6) small-break loss-of-coolant accident (LOCA), and (7) large-break LOCA. Five simulator benchmark sessions have been completed. Each session identified simulator capabilities and limitations that needed correction. This paper discusses results from the latest large-break LOCA case.

Loomis, J.N.; Fernandez, R.T.

1987-01-01T23:59:59.000Z

248

Purchasing Power Parity: Error Correction Models and Structural Breaks  

Science Journals Connector (OSTI)

This paper examines purchasing power parity (PPP) behavior using error correction models (ECM) and allowing for structural breaks. We distinguish four different objectives: first, this paper examines which var...

Amalia Morales Zumaquero; Rodrigo Peruga Urrea

2002-01-01T23:59:59.000Z

249

Calculation of the Limiting CESSAR Steam Line Break Transients  

Science Journals Connector (OSTI)

Argonne National Laboratory (ANL), under contract to the Nuclear Regulatory Commission, performed audit calculations of the limiting and Steam Line Break (SLB) [1] transient presented in the CESSAR FSAR. The r...

G. B. Peeler; D. L. Caraher; J. Guttmann

1984-01-01T23:59:59.000Z

250

New Mechanisms of Gauge-Mediated Supersymmetry Breaking  

E-Print Network (OSTI)

We introduce new mechanisms for the communication of supersymmetry breaking via gauge interactions. These models do not require complicated dynamics to induce a nonvanishing F term for a singlet. The first class of models communicates supersymmetry breaking to the visible sector through a ``mediator" field that transforms under both a messenger gauge group of the dynamical supersymmetry breaking sector and the standard model gauge group. This model has distinctive phenomenology; in particular, the scalar superpartners should be heavier by at least an order of magnitude than the gaugino superpartners. The second class of models has phenomenology more similar to the ``standard" messenger sectors. A singlet is incorporated, but the model does not require complicated mechanisms to generate a singlet F term. The role of the singlet is to couple fields from the dynamical symmetry breaking sector to fields transforming under the standard model gauge group. We also mention a potential solution to the $\\mu$ problem.

Lisa Randall

1996-12-19T23:59:59.000Z

251

Some Evidence of Colinear Wind Stress and Wave Breaking  

Science Journals Connector (OSTI)

Data collected during the Surface Waves and Processes Program are employed to investigate a possible interrelation between wind stress and surface wave breaking. From comparison of data from 15 half-hour long time segments, the directions of the ...

Karl F. Rieder; Jerome A. Smith; Robert A. Weller

1996-11-01T23:59:59.000Z

252

Symmetry Breaking of H2 Dissociation by a Single Photon  

NLE Websites -- All DOE Office Websites (Extended Search)

Symmetry Breaking of H2 Dissociation by a Single Photon Print A single hydrogen (or deuterium) molecule consists of only two protons (deuterons) and two electrons and is perfectly...

253

Statistical estimation of water distribution system pipe break risk  

E-Print Network (OSTI)

The deterioration of pipes in urban water distribution systems is of concern to water utilities throughout the world. This deterioration generally leads to pipe breaks and leaks, which may result in reduction in the water-carrying capacity...

Yamijala, Shridhar

2009-05-15T23:59:59.000Z

254

Sets of Symmetry Breaking Constraints Barbara M. Smith  

E-Print Network (OSTI)

Sets of Symmetry Breaking Constraints Barbara M. Smith Cork Constraint Computation Centre, University College Cork, Ireland b.m.smith@4c.ucc.ie Abstract [Puget, 2004] has shown that if the symmetry

Smith, Barbara M.

255

Energy Dissipation of Unsteady Wave Breaking on Currents  

Science Journals Connector (OSTI)

Energy dissipation for unsteady deep-water breaking in wave groups on following and opposing currents, including partial wave-blocking conditions, was investigated by detailed laboratory measurements. A range of focusing wave conditions, ...

Aifeng Yao; Chin H. Wu

2004-10-01T23:59:59.000Z

256

Protein Bridges DNA Base and Nucleotide Excision Repair Pathways  

NLE Websites -- All DOE Office Websites (Extended Search)

Protein Bridges DNA Base and Protein Bridges DNA Base and Nucleotide Excision Repair Pathways Protein Bridges DNA Base and Nucleotide Excision Repair Pathways Print Wednesday, 28 October 2009 00:00 Alkyltransferase proteins (AGT) protect cells from the biological effects of DNA damage caused by the addition of alkyl groups (alkylation). Alkyltransferase-like proteins (ATLs) can do the same, but they lack the reactive cysteine residue that allows the alkyltransferase function, and the mechanism for cell protection has remained unknown. To address this mystery, a British-American team lead by researchers at the Scripps Research Institute recently applied a combination of x-ray structural, biochemical, and genetic studies to ATLs in the yeast Schizosaccharomyces pombe without and with damaged DNA. By showing how a process called non-enzymatic nucleotide flipping activates ATL-initiated DNA repair, their results may improve our understanding of genomic integrity and responses to DNA damage relevant to pathogens and cancer development.

257

Simple Maintenance Saves Costly Furnace Repair/Replacement | Department of  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Simple Maintenance Saves Costly Furnace Repair/Replacement Simple Maintenance Saves Costly Furnace Repair/Replacement Simple Maintenance Saves Costly Furnace Repair/Replacement January 6, 2010 - 8:26am Addthis Chris Stewart Senior Communicator at DOE's National Renewable Energy Laboratory For the past few weeks, my forced-air gas furnace has been on the fritz. I blame this on the fact that I haven't been as diligent as I should have been with regular furnace maintenance, which includes: Checking the condition of the vent connection pipe and chimney Checking the physical integrity of the heat exchanger Adjusting the controls to provide optimum water and air temperature settings for both efficiency and comfort Having a technician perform a combustion-efficiency test Checking the combustion chamber for cracks. Testing for carbon monoxide

258

Protein Bridges DNA Base and Nucleotide Excision Repair Pathways  

NLE Websites -- All DOE Office Websites (Extended Search)

Protein Bridges DNA Base and Nucleotide Excision Repair Pathways Print Protein Bridges DNA Base and Nucleotide Excision Repair Pathways Print Alkyltransferase proteins (AGT) protect cells from the biological effects of DNA damage caused by the addition of alkyl groups (alkylation). Alkyltransferase-like proteins (ATLs) can do the same, but they lack the reactive cysteine residue that allows the alkyltransferase function, and the mechanism for cell protection has remained unknown. To address this mystery, a British-American team lead by researchers at the Scripps Research Institute recently applied a combination of x-ray structural, biochemical, and genetic studies to ATLs in the yeast Schizosaccharomyces pombe without and with damaged DNA. By showing how a process called non-enzymatic nucleotide flipping activates ATL-initiated DNA repair, their results may improve our understanding of genomic integrity and responses to DNA damage relevant to pathogens and cancer development.

259

RECORD OF CATEGORICAL EXCLUSION DETERMINATION Hygiene Substation Access Road Repairs  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Hygiene Substation Access Road Repairs Hygiene Substation Access Road Repairs A. Brief Description of Proposal: Western Area Power Administration proposes to repair the access road/driveway into the Hygiene Substation. There is a low spot in the middle of the driveway that impedes maneuvering oflarge maintenance equipment in and out of the yard. Western plans to fill the low spot with road base and crumbled concrete fill. The Hygiene Substation is located in Boulder County, approximately 2 miles northwest of Longmont, CO (T3N, R69W, Sec 18). All proposed construction will occur within the existing, disturbed substation access road/driveway. B. Number and Title of tile Categorical Exclusion Being Applied: (See text in 10 CFR 1021, Subpart D.) BI.3 Routine maintenance activities and custodial services for buildings, structures,

260

Protein Bridges DNA Base and Nucleotide Excision Repair Pathways  

NLE Websites -- All DOE Office Websites (Extended Search)

Protein Bridges DNA Base and Nucleotide Excision Repair Pathways Print Protein Bridges DNA Base and Nucleotide Excision Repair Pathways Print Alkyltransferase proteins (AGT) protect cells from the biological effects of DNA damage caused by the addition of alkyl groups (alkylation). Alkyltransferase-like proteins (ATLs) can do the same, but they lack the reactive cysteine residue that allows the alkyltransferase function, and the mechanism for cell protection has remained unknown. To address this mystery, a British-American team lead by researchers at the Scripps Research Institute recently applied a combination of x-ray structural, biochemical, and genetic studies to ATLs in the yeast Schizosaccharomyces pombe without and with damaged DNA. By showing how a process called non-enzymatic nucleotide flipping activates ATL-initiated DNA repair, their results may improve our understanding of genomic integrity and responses to DNA damage relevant to pathogens and cancer development.

Note: This page contains sample records for the topic "double-strand break repair" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


261

Protein Bridges DNA Base and Nucleotide Excision Repair Pathways  

NLE Websites -- All DOE Office Websites (Extended Search)

Protein Bridges DNA Base and Nucleotide Excision Repair Pathways Print Protein Bridges DNA Base and Nucleotide Excision Repair Pathways Print Alkyltransferase proteins (AGT) protect cells from the biological effects of DNA damage caused by the addition of alkyl groups (alkylation). Alkyltransferase-like proteins (ATLs) can do the same, but they lack the reactive cysteine residue that allows the alkyltransferase function, and the mechanism for cell protection has remained unknown. To address this mystery, a British-American team lead by researchers at the Scripps Research Institute recently applied a combination of x-ray structural, biochemical, and genetic studies to ATLs in the yeast Schizosaccharomyces pombe without and with damaged DNA. By showing how a process called non-enzymatic nucleotide flipping activates ATL-initiated DNA repair, their results may improve our understanding of genomic integrity and responses to DNA damage relevant to pathogens and cancer development.

262

The repair and rehabilitation method of Tokyo gas  

SciTech Connect

It has been an important matter for gas utilities to maintain their pipelines easily and economically. But pipes that have become old suffer from progressive corrosion, and as a result of vibration caused by traffic or the effects of land subsidence, joints can become loosened. To repair pipes that have suffered in such ways, the usual method to do is to dig them up and replace them with new ones. But this method requires a large amount of excavation, so the work takes a long time to complete and is costly. To deal with these problems, Tokyo Gas developed number of repair and rehabilitation methods to permit renewal of pipelines without replacing them. In this paper, I would like to introduce our repair and rehabilitation methods for low-pressure pipeline. 4 refs., 9 figs., 4 tabs.

Yagi, Shuichi [Tokyo Gas Co., Ltd. (Japan)

1996-08-01T23:59:59.000Z

263

Additive manufacturing for in situ repair of osteochondral defects  

Science Journals Connector (OSTI)

Tissue engineering holds great promise for injury repair and replacement of defective body parts. While a number of techniques exist for creating living biological constructs in vitro, none have been demonstrated for in situ repair. Using novel geometric feedback-based approaches and through development of appropriate printing-material combinations, we demonstrate the in situ repair of both chondral and osteochondral defects that mimic naturally occurring pathologies. A calf femur was mounted in a custom jig and held within a robocasting-based additive manufacturing (AM) system. Two defects were induced: one a cartilage-only representation of a grade IV chondral lesion and the other a two-material bone and cartilage fracture of the femoral condyle. Alginate hydrogel was used for the repair of cartilage; a novel formulation of demineralized bone matrix was used for bone repair. Repair prints for both defects had mean surface errors less than 0.1 mm. For the chondral defect, 42.8 ± 2.6% of the surface points had errors that were within a clinically acceptable error range; however, with 1 mm path planning shift, an estimated ~75% of surface points could likely fall within the benchmark envelope. For the osteochondral defect, 83.6 ± 2.7% of surface points had errors that were within clinically acceptable limits. In addition to implications for minimally invasive AM-based clinical treatments, these proof-of-concept prints are some of the only in situ demonstrations to-date, wherein the substrate geometry was unknown a priori. The work presented herein demonstrates in situ AM, suggests potential biomedical applications and also explores in situ-specific issues, including geometric feedback, material selection and novel path planning techniques.

Daniel L Cohen; Jeffrey I Lipton; Lawrence J Bonassar; Hod Lipson

2010-01-01T23:59:59.000Z

264

Repairs for damaged bolt holes in continuous fiber reinforced plastics  

E-Print Network (OSTI)

repair method for damaged bolt holes in such composites. Bolt holes in three types of graphite-epoxy were purposely damaged and then repaired. Each was tested to characterize its static and fatigue behavior. The tests used a special fixture to simulate... composite joints . Bearing-bypass ratio can change the failure mode of bolted composite joints 7 Drilling defects in graphite-epoxy coupons 12 26 The exit side of a IM7/8551-7A 18 ply tape coupon showing the damage due to drilling 27 9 Resin filled...

Copps, Kevin Daniel

2012-06-07T23:59:59.000Z

265

Ice plug employed on subsea pipeline bend during repair  

SciTech Connect

The first controlled-temperature ice plug in the bend of an offshore gas trunkline has been carried out for Phillips Petroleum Co. Norway on its Norpipe A.S. platform in the German sector of the North Sea. The procedure was part of a subsea valve repair operation. The ice plug was successfully formed offshore and tested to a differential pressure of 1,450 psi. Repair of two valves required only 5 days during which time gas production was operating at close to 50--60% via the platform bypass, says the service company. The paper discusses the procedure.

NONE

1997-12-22T23:59:59.000Z

266

A Comparison of Measured and Predicted Wave-Impact Pressures from Breaking and Non-breaking Waves  

E-Print Network (OSTI)

Impact loads from waves on vessels and coastal structures are complex and may involve wave breaking, which has made these loads difficult to estimate numerically or empirically. Results from previous experiments have shown a wide range of forces and pressures measured from breaking and nonbreaking waves, with no clear trend between wave characteristics and the localized forces and pressures that they generate. In 2008, a canonical breaking wave impact data set was obtained at the Naval Surface Warfare Center, Carderock Division, by measuring the distribution of impact pressures of incident nonbreaking and breaking waves on one face of a cube. This experimental effort was sponsored by the Office of Naval Research (ONR), under the Dynamics of Interacting Platforms Program, Program Manager Dr. Ron Joslin. The effects of wave height, wavelength, face orientation, face angle, and submergence depth were investigated. Additionally, a limited number of runs were made at low forward speeds, ranging from about 0.5 to 2...

Fullerton, Anne M; Brewton, Susan; Brucker, Kyle A; O'Shea, Thomas T; Dommermuth, Douglas G

2014-01-01T23:59:59.000Z

267

Guardian | Cracking future for fabric that heals its own breaks Cracking future for fabric that heals its own breaks  

E-Print Network (OSTI)

that heals its own breaks Ian Sample looks at how self-healing materials inspired by nature could make cars. Manufacturing costs are dominated by the need to make high- performance materials as defect-free as possible

Aksay, Ilhan A.

268

Polymorphisms in DNA repair genes and risk of non-Hodgkin lymphoma among women in Connecticut  

Science Journals Connector (OSTI)

Several hereditary syndromes characterized by defective DNA repair are associated with high risk of non-Hodgkin lymphoma (NHL). To explore whether common polymorphisms in DNA repair genes affect risk of NHL in th...

Min Shen; Tongzhang Zheng; Qing Lan; Yawei Zhang; Shelia H. Zahm…

2006-07-01T23:59:59.000Z

269

Subsea Repair of Concrete Weight Coating on 30? North Sea Pipeline  

Science Journals Connector (OSTI)

The 30? North Sea Gas Pipeline in the Danish sector had suffered damage ... trawlboards and other heavy fishing gear. To repair this damage several methods of repair were considered to find one which could ... pr...

Erik Reker

1991-01-01T23:59:59.000Z

270

Minimum thickness for circumferential sleeve repair fillet welds in corroded gas pipelines  

Science Journals Connector (OSTI)

The minimum weldable pipe wall thickness for sleeve repair welds is numerically assessed in this work, as a function of pressure during the welding operations of a corroded gas pipeline, according to the approach by Battelle. The minimum weldable thickness is found to increase when the flow rate of the transported gas in the section being repaired increases. Integrity of the repairs is assessed, and alternative measures to momentarily increase the flow in the area of the repair are evaluated.

A.P Cisilino; M.D Chapetti; J.L Otegui

2002-01-01T23:59:59.000Z

271

Remote Connection Systems for Subsea Pipeline Tie-Ins and Repairs  

Science Journals Connector (OSTI)

The MORGRIP coupling has been used on applications both Topside and for subsea pipeline tie ins and repairs.

Derek Cruickshank; Phil Maxted

1994-01-01T23:59:59.000Z

272

Reduced COX-2 Protein in Colorectal Cancer with Defective Mismatch Repair  

Science Journals Connector (OSTI)

...Protein in Colorectal Cancer with Defective Mismatch Repair 1 1 This work...typic characteristics of defective DNA mismatch repair express...may arise in the setting of defective DNA mismatch repair. INTRODUCTION...sporadic colorectal adenomas and car cinomas (6-7) and in FAP...

William E. Karnes, Jr.; Rebecca Shattuck-Brandt; Lawrence J. Burgart; Raymond N. DuBois; David J. Tester; Julie M. Cunningham; Cheong-Yong Kim; Shannon K. McDonnell; Daniel J. Schaid; and Stephen N. Thibodeau

1998-12-01T23:59:59.000Z

273

Repair of UV Dimers in Skin DNA of Patients with Basal Cell Carcinoma  

Science Journals Connector (OSTI)

...carcinoma have a reduced capacity to repair UV-induced...after a brief exposure to solar-simulated UV radiation...Therefore, a reduced capacity to repair photolesions...induced in human skin by solar-simulating UV radiation...Grossman L. DNA repair capacity for ultraviolet light-induced...

Dan Segerbäck; Malgorzata Strozyk; Erna Snellman; and Kari Hemminki

2008-09-01T23:59:59.000Z

274

Development and Field-Test Validation of an Assay for DNA Repair in Circulating Human Lymphocytes  

Science Journals Connector (OSTI)

...as a result of the reduced capacity of patients' cells to repair...Since the UV component of solar radiation exists as the predominant...study. orientation of reduced capacity for DNA repair of UV-irradi...of estrus on the DNA repair capacity of these subjects. The effect...

William F. Athas; Mohammad A. Hedayati; Genevieve M. Matanoski; Evan R. Farmer; and Lawrence Grossman

1991-11-01T23:59:59.000Z

275

Inhibition of Short Patch and Long Patch Base Excision Repair by an Oxidized Abasic Site  

Science Journals Connector (OSTI)

Inhibition of Short Patch and Long Patch Base Excision Repair by an Oxidized Abasic Site ... Inhibition of DNA polymerase ?’s lyase function is accompanied by inactivation of its DNA polymerase activity as well, which prevents long patch base excision repair of DOB. ... Overall, DOB is highly refractory to short patch and long patch base excision repair. ...

Lirui Guan; Katarzyna Bebenek; Thomas A. Kunkel; Marc M. Greenberg

2010-10-20T23:59:59.000Z

276

Corrosion Repair and Corrosion Protection of Reinforced Concrete Structures in  

E-Print Network (OSTI)

11/14/2014 1 Corrosion Repair and Corrosion Protection of Reinforced Concrete Structures in Pulp and Paper Mills Vector Construction / Vector Corrosion Technologies www.vectorgroup.com www.vector-corrosion.com Presentation Outline · Introduction · Corrosion of Reinforced Concrete Structures · Protection of Chests

Das, Suman

277

HOW TO REPAIR A SECOND-ORDER SURFACE FOR COMPUTER  

E-Print Network (OSTI)

1 HOW TO REPAIR A SECOND-ORDER SURFACE FOR COMPUTER EXPERIMENTS BY KRIGING Astrid JOURDAN and analysis of computer experiments have been widely investigated in the past decade. Two approaches seem describes and compares them briefly in order to present a methodology for computer experiments based

Paris-Sud XI, Université de

278

Workshop Proceedings: Life Assessment and Repair of Steam Turbine Casings  

SciTech Connect

With the trend toward operating steam turbines far beyond their design lives, utilities need methods for assessing and extending the lives of the turbine casings. Consolidating and publishing industry experience on this subject will help utilities make run/replace decisions, select appropriate repair techniques, implement operational changes, and select new designs to meet future needs.

None

1986-07-01T23:59:59.000Z

279

Development/Plasticity/Repair Identification of Nicotinic Acetylcholine Receptor Recycling  

E-Print Network (OSTI)

Development/Plasticity/Repair Identification of Nicotinic Acetylcholine Receptor Recycling and Its, University of Michigan, Ann Arbor, Michigan 48109 In the CNS, receptor recycling is critical for synaptic plasticity; however, the recycling of receptors has never been observed at peripheral synapses. Using a novel

Alford, Simon

280

USDA Grants to Help Repair Housing in Rural Areas  

Energy.gov (U.S. Department of Energy (DOE))

The U.S. Department of Agriculture (USDA) is accepting applications for the Housing Preservation Grant program, which provides funding to repair low-income housing, rental properties, or co-op owned housing. Eligible sponsors include state agencies, local government, Tribes, and nonprofits.

Note: This page contains sample records for the topic "double-strand break repair" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


281

DNA Damage Repair Pathways in Cancer Stem Cells  

Science Journals Connector (OSTI)

...reads to the genome was performed using the AB BioScope 1.2 Pipeline and outputs imported into Partek Genomics Suite for analysis...results reveals upregulation of genes and miRNAs involved in DNA repair, angiogenesis, and inhibitors of Estrogen Receptor-alpha...

Marcello Maugeri-Saccà; Monica Bartucci; and Ruggero De Maria

2012-08-01T23:59:59.000Z

282

Thermosetting Polymer-Matrix Composites for Strucutral Repair Applications  

SciTech Connect

Several classes of thermosetting polymer matrix composites were evaluated for use in structural repair applications. Initial work involved the characterization and evaluation of woven carbon fiber/epoxy matrix composites for structural pipeline repair. Cyanate ester resins were evaluated as a replacement for epoxy in composites for high-temperature pipe repair applications, and as the basis for adhesives for resin infusion repair of high-temperature composite materials. Carbon fiber/cyanate ester matrix composites and fumed silica/cyanate ester nanocomposites were evaluated for their thermal, mechanical, viscoelastic, and rheological properties as they relate to their structure, chemistry, and processing characteristics. The bisphenol E cyanate ester under investigation possesses a high glass transition temperature, excellent mechanical properties, and unique ambient temperature processability. The incorporate of fumed silica served to enhance the mechanical and rheological properties of the polymer and reduce thermal expansion without sacrificing glass transition or drastically altering curing kinetics. Characterization of the composites included dynamic mechanical analysis, thermomechanical analysis, differential scanning calorimetry, thermogravimetric analysis, rheological and rheokinetic evaluation, and transmission electron microscopy.

William Kirby Goertzen

2007-12-01T23:59:59.000Z

283

Are high-redshift DLA galxies Lyman-break galaxies?  

E-Print Network (OSTI)

We use deep HST STIS and NICMOS images of three spectroscopically confirmed galaxy counterparts of high-redshift damped Ly-alpha (DLA) absorbers (one of which is a new discovery) to test the hypothesis that high-redshift DLA galaxies are Lyman-break galaxies. If this hypothesis is correct the emission properties of DLA galaxies must lie within the range of emission properties measured for Lyman-break galaxies of similar absolute magnitude. This will be true regardless of selection biases in the sample of detected DLA galaxies. We test this prediction using several emission properties: half-light radius, radial profile (Sersic n parameter), optical-to-near-infrared colour, morphology, Ly alpha emission equivalent width, and Ly alpha emission velocity structure. In all cases the measured values for the DLA galaxies lie within the range measured for the population of Lyman-break galaxies. None of the measurements is in conflict with the prediction. We conclude that the measured emission properties of the three DLA galaxies studied here are consistent with the conjecture that high-redshift DLA galaxies are Lyman-break galaxies. We show that this result does not conflict with the observation that the few high-redshift DLA galaxies discovered are mostly fainter than spectroscopically confirmed L* Lyman-break galaxies.

P. Møller; S. J. Warren; S. M. Fall; J. U. Fynbo; P. Jakobsen

2002-03-21T23:59:59.000Z

284

SB Electronics Breaks Ground on New Factory | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

SB Electronics Breaks Ground on New Factory SB Electronics Breaks Ground on New Factory SB Electronics Breaks Ground on New Factory April 29, 2010 - 5:22pm Addthis U.S. Rep. Peter Welch (from left), Vermont Lt. Gov. Brian Dubie, SBE board member Win Hunter, SBE board chair Stan Fishkin, Assi U.S. Rep. Peter Welch (from left), Vermont Lt. Gov. Brian Dubie, SBE board member Win Hunter, SBE board chair Stan Fishkin, Assi Paul Lester Communications Specialist, Office of Energy Efficiency and Renewable Energy A Vermont company broke ground on a new factory that will produce cutting-edge technology for electric and hybrid cars and create more than 100 jobs. The event ushering in SB Electronics' power ring capacitor facility in Barre was attended by Vermont Gov. Jim Douglas and federal, state and local

285

Deputy Secretary Poneman Attends Ground Breaking at Tennessee Advanced  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Attends Ground Breaking at Tennessee Attends Ground Breaking at Tennessee Advanced Vehicle Battery Plant Deputy Secretary Poneman Attends Ground Breaking at Tennessee Advanced Vehicle Battery Plant May 26, 2010 - 12:00am Addthis Smyrna, TN - Today, U.S. Deputy Secretary of Energy Daniel Poneman participated in the groundbreaking ceremony for Nissan North America's advanced battery manufacturing facility in Smyrna, Tennessee. This past January the Department closed a $1.4 billion loan with Nissan North America to retool the Smyrna factory to build advanced electric automobiles and an advanced battery manufacturing facility. "I'm excited about the future we have begun to build here today -- a future where America's workers have good jobs, making clean cars that will reduce our dependence on oil and help us transition to a clean energy economy,"

286

Symmetry Breaking of H2 Dissociation by a Single Photon  

NLE Websites -- All DOE Office Websites (Extended Search)

Symmetry Breaking of H2 Dissociation by a Single Photon Print Symmetry Breaking of H2 Dissociation by a Single Photon Print A single hydrogen (or deuterium) molecule consists of only two protons (deuterons) and two electrons and is perfectly symmetric. Linearly polarized photons are similarly symmetric. So one might think that the angular distribution of photoelectrons resulting from photoionization of the molecule by the photon accompanied by dissociation into a hydrogen atom and a hydrogen ion would itself be symmetric. However, an international team of researchers from Germany, Spain, and the U.S. has now shown that this need not be the case. When there are multiple quantum paths for the process, interference between waves in the coherent superposition of electron states (which exists when the molecular fragments are still close together) skews the distribution by breaking the molecular symmetry.

287

Symmetry Breaking of H2 Dissociation by a Single Photon  

NLE Websites -- All DOE Office Websites (Extended Search)

Symmetry Breaking of H2 Dissociation by a Single Photon Print Symmetry Breaking of H2 Dissociation by a Single Photon Print A single hydrogen (or deuterium) molecule consists of only two protons (deuterons) and two electrons and is perfectly symmetric. Linearly polarized photons are similarly symmetric. So one might think that the angular distribution of photoelectrons resulting from photoionization of the molecule by the photon accompanied by dissociation into a hydrogen atom and a hydrogen ion would itself be symmetric. However, an international team of researchers from Germany, Spain, and the U.S. has now shown that this need not be the case. When there are multiple quantum paths for the process, interference between waves in the coherent superposition of electron states (which exists when the molecular fragments are still close together) skews the distribution by breaking the molecular symmetry.

288

Deputy Secretary Poneman Attends Ground Breaking at Tennessee Advanced  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Poneman Attends Ground Breaking at Tennessee Poneman Attends Ground Breaking at Tennessee Advanced Vehicle Battery Plant Deputy Secretary Poneman Attends Ground Breaking at Tennessee Advanced Vehicle Battery Plant May 26, 2010 - 12:00am Addthis Smyrna, TN - Today, U.S. Deputy Secretary of Energy Daniel Poneman participated in the groundbreaking ceremony for Nissan North America's advanced battery manufacturing facility in Smyrna, Tennessee. This past January the Department closed a $1.4 billion loan with Nissan North America to retool the Smyrna factory to build advanced electric automobiles and an advanced battery manufacturing facility. "I'm excited about the future we have begun to build here today -- a future where America's workers have good jobs, making clean cars that will reduce our dependence on oil and help us transition to a clean energy economy,"

289

Wind Power Reliability: Breaking Down a Barrier | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Wind Power Reliability: Breaking Down a Barrier Wind Power Reliability: Breaking Down a Barrier Wind Power Reliability: Breaking Down a Barrier June 25, 2010 - 12:16pm Addthis EnerNex Corporation is developing documentation and validating generic wind turbine and plant models that test reliability. | File photo EnerNex Corporation is developing documentation and validating generic wind turbine and plant models that test reliability. | File photo Stephen Graff Former Writer & editor for Energy Empowers, EERE The steady increase of wind power on the grid presents new challenges for power system operators charged with making sure the grid stays up and running. "We need to ensure that we are going down a path that will lead to better reliability [with wind power]," said Bob Zavadil, an executive vice

290

Breaking a Pocket of Resistance in the Fight Against Cancer  

NLE Websites -- All DOE Office Websites (Extended Search)

Breaking a Pocket of Resistance in Breaking a Pocket of Resistance in the Fight Against Cancer Breaking a Pocket of Resistance in the Fight Against Cancer Print Thursday, 12 December 2013 11:55 ras protein The new class of inhibitors interacts with a specific mutation (Glycine to Cysteine) associated with a number of types of lung cancer. Mutations in the protein K-RAS are a very common cause for certain types of human cancers and are generally associated with a poor response to standard therapies. RAS, an abbreviation of Rat Sarcoma, is a nucleotide binding protein that responds to chemical signals (nucleotides). When in the "on" state, RAS activates other proteins, resulting in a cascade of biochemical processes; in the "off" state, RAS remains inactive. Mutations in the RAS

291

Energy Department Training Breaks New Ground | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Training Breaks New Ground Training Breaks New Ground Energy Department Training Breaks New Ground December 3, 2013 - 1:00pm Addthis The Federal Energy Management Program is now an authorized provider of continuing education units from the International Association for Continuing Education and Training. Watch the video above to learn more. Timothy Unruh FEMP Program Manager High quality education for federal government employees helps streamline operations, improve services, and ensure that taxpayer dollars are spent wisely. This is why I am pleased to announce the Energy Department's Federal Energy Management Program (FEMP) has earned the designation as an "Authorized Provider" of continuing education units (CEUs) by the prestigious International Association for Continuing Education and Training

292

NREL: News Feature - NREL Breaks Down Walls for Biofuels  

NLE Websites -- All DOE Office Websites (Extended Search)

NREL Breaks Down Walls for Biofuels NREL Breaks Down Walls for Biofuels November 30, 2009 Researchers at the National Renewable Energy Laboratory (NREL) and ethanol producers are racing to come up with ways to make ethanol from cellulosic biomass that are cheaper and easier to produce than current methods. But they are hitting a wall. Cell walls in plants are making the production of cellulosic ethanol a challenge. So researchers are creating their own computer program to help model and break down the tiny fibers of cellulose - or fibrils - found in plant cells. Although ethanol is becoming more available to consumers, NREL is working closely with the U.S. Department of Energy (DOE) to meet a quickly approaching goal to produce competitively priced ethanol for $1.50 per gallon by 2012. Why the rush? DOE believes this is the price at which

293

Lorentz symmetry breaking as a quantum field theory regulator  

Science Journals Connector (OSTI)

Perturbative expansions of quantum field theories typically lead to ultraviolet (short-distance) divergences requiring regularization and renormalization. Many different regularization techniques have been developed over the years, but most regularizations require severe mutilation of the logical foundations of the theory. In contrast, breaking Lorentz invariance, while it is certainly a radical step, at least does not damage the logical foundations of the theory. I shall explore the features of a Lorentz symmetry breaking regulator in a simple polynomial scalar field theory and discuss its implications. In particular, I shall quantify just “how much” Lorentz symmetry breaking is required to fully regulate the quantum theory and render it finite. This scalar field theory provides a simple way of understanding many of the key features of Ho?ava’s recent article [Phys. Rev. D 79, 084008 (2009)] on 3+1 dimensional quantum gravity.

Matt Visser

2009-07-23T23:59:59.000Z

294

Ground Breaking of Blythe Solar Power Project | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Ground Breaking of Blythe Solar Power Project Ground Breaking of Blythe Solar Power Project Ground Breaking of Blythe Solar Power Project June 20, 2011 - 2:16pm Addthis Secretary Chu Secretary Chu Former Secretary of Energy What will the project do? Blythe Solar Power Project will generate 1,000 megawatts of solar power, enough to power more than 300,000 single-family homes a year. Back in April, I had the pleasure of announcing that the Department of Energy had extended our largest conditional loan guarantee for a solar project - $2.1 billion to support a concentrating solar thermal power plant near Blythe, California. Last Friday, the Blythe Solar Power Project broke ground, beginning construction of a project that upon completion will generate 1,000 megawatts of solar power, enough to power more than 300,000

295

Reddit AMA: Marius Stan, energy researcher and Breaking Bad actor |  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Reddit AMA: Marius Stan, energy researcher and Breaking Bad actor Reddit AMA: Marius Stan, energy researcher and Breaking Bad actor Reddit AMA: Marius Stan, energy researcher and Breaking Bad actor February 14, 2013 - 12:36pm Addthis Marius Stan, computational energy scientist from Argonne National Lab, submits his verification photo as part of an informal question and answer session with Internet users on the Ask Me Anything section of the social website Reddit. Marius Stan, computational energy scientist from Argonne National Lab, submits his verification photo as part of an informal question and answer session with Internet users on the Ask Me Anything section of the social website Reddit. Michael Hess Michael Hess Former Digital Communications Specialist, Office of Public Affairs What is an Reddit AMA? AMA stands for "Ask me anything."

296

Margins in high temperature leak-before-break assessments  

SciTech Connect

Developments in the defect assessment procedure R6 to include high-temperature mechanisms in Leak-before-Break arguments are described. In particular, the effect of creep on the time available to detect a leak and on the crack opening area, and hence leak rate, is discussed. The competing influence of these two effects is emphasized by an example. The application to Leak-before-Break of the time-dependent failure assessment diagram approach for high temperature defect assessment is then outlined. The approach is shown to be of use in assessing the erosion of margins by creep.

Budden, P.J.; Hooton, D.G.

1997-04-01T23:59:59.000Z

297

Charge and CP symmetry breaking in two Higgs doublet models  

E-Print Network (OSTI)

We show that, for the most generic model with two Higgs doublets possessing a minimum that preserves the $U(1)_{em}$ symmetry, charge breaking (CB) cannot occur. If CB does not occur, the potential could have two different minima, and there is in principle no general argument to show which one is the deepest. The depth of the potential at a stationary point that breaks CB or CP, relative to the $U(1)_{em}$ preserving minimum, is proportional to the squared mass of the charged or pseudoscalar Higgs, respectively.

A. Barroso; P. M. Ferreira; R. Santos

2005-11-07T23:59:59.000Z

298

Parg deficiency confers radio-sensitization through enhanced cell death in mouse ES cells exposed to various forms of ionizing radiation  

SciTech Connect

Highlights: •Parg{sup ?/?} ES cells were more sensitive to ?-irradiation than Parp-1{sup ?/?} ES cells. •Parg{sup ?/?} cells were more sensitive to carbon-ion irradiation than Parp-1{sup ?/?} cells. •Parg{sup ?/?} cells showed defects in DSB repair after carbon-ion irradiation. •PAR accumulation was enhanced after carbon-ion irradiation compared to ?-irradiation. -- Abstract: Poly(ADP-ribose) glycohydrolase (Parg) is the main enzyme involved in poly(ADP-ribose) degradation. Here, the effects of Parg deficiency on sensitivity to low and high linear-energy-transfer (LET) radiation were investigated in mouse embryonic stem (ES) cells. Mouse Parg{sup ?/?} and poly(ADP-ribose) polymerase-1 deficient (Parp-1{sup ?/?}) ES cells were used and responses to low and high LET radiation were assessed by clonogenic survival and biochemical and biological analysis methods. Parg{sup ?/?} cells were more sensitive to ?-irradiation than Parp-1{sup ?/?} cells. Transient accumulation of poly(ADP-ribose) was enhanced in Parg{sup ?/?} cells. Augmented levels of phosphorylated H2AX (?-H2AX) from early phase were observed in Parg{sup ?/?} ES cells. The induction level of p53 phophorylation at ser18 was similar in wild-type and Parp-1{sup ?/?} cells and apoptotic cell death process was mainly observed in the both genotypes. These results suggested that the enhanced sensitivity of Parg{sup ?/?} ES cells to ?-irradiation involved defective repair of DNA double strand breaks. The effects of Parg and Parp-1 deficiency on the ES cell response to carbon-ion irradiation (LET13 and 70 keV/?m) and Fe-ion irradiation (200 keV/?m) were also examined. Parg{sup ?/?} cells were more sensitive to LET 70 keV/?m carbon-ion irradiation than Parp-1{sup ?/?} cells. Enhanced apoptotic cell death also accompanied augmented levels of ?-H2AX in a biphasic manner peaked at 1 and 24 h. The induction level of p53 phophorylation at ser18 was not different between wild-type and Parg{sup ?/?} cells. The augmented level of poly(ADP-ribose) accumulation was noted after carbon-ion irradiation compared to ?-irradiation even in the wild-type cells. An enhanced poly(ADP-ribose) accumulation was further observed in Parg{sup ?/?} cells. Both Parg{sup ?/?} cells and Parp-1{sup ?/?} cells did not show sensitization to Fe-ion irradiation. Parg deficiency sensitizes mouse ES cells to a wide therapeutic range of LET radiation through the effects on DNA double strand break repair responses and enhanced cell death.

Shirai, Hidenori; Fujimori, Hiroaki [Division of Genome Stability Research, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan)] [Division of Genome Stability Research, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan); Gunji, Akemi [Biochemistry Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan)] [Biochemistry Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan); Maeda, Daisuke [Biochemistry Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan) [Biochemistry Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan); ADP-Ribosylation in Oncology Project, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan); Hirai, Takahisa [Division of Genome Stability Research, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan) [Division of Genome Stability Research, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan); Department of Radiation Oncology, Juntendo University Faculty of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421 (Japan); Poetsch, Anna R. [ADP-Ribosylation in Oncology Project, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan)] [ADP-Ribosylation in Oncology Project, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan); Harada, Hiromi [Division of Genome Stability Research, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan)] [Division of Genome Stability Research, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan); Yoshida, Tomoko [Biochemistry Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan) [Biochemistry Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan); Kyoritsu College of Pharmacy, 1-5-30 Shibakoen, Minatoku, Tokyo 105-8512 (Japan); Sasai, Keisuke [Department of Radiation Oncology, Juntendo University Faculty of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421 (Japan)] [Department of Radiation Oncology, Juntendo University Faculty of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421 (Japan); Okayasu, Ryuichi [International Open Laboratory, National Institute of Radiological Science, 4-9-1 Anagawa, Inage, Chiba 263-8555 (Japan)] [International Open Laboratory, National Institute of Radiological Science, 4-9-1 Anagawa, Inage, Chiba 263-8555 (Japan); Masutani, Mitsuko, E-mail: mmasutan@ncc.go.jp [Division of Genome Stability Research, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan) [Division of Genome Stability Research, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan); Biochemistry Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan); ADP-Ribosylation in Oncology Project, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan)

2013-05-24T23:59:59.000Z

299

Solar Cells, Wound Repair Winning GVC Technologies | ornl.gov  

NLE Websites -- All DOE Office Websites (Extended Search)

Solar Cells, Wound Repair Winning GVC Technologies Solar Cells, Wound Repair Winning GVC Technologies March 26, 2010 Graduate student teams from the University of Arkansas and the University of Maryland earned first place in the energy and security categories at the 2010 GVC hosted by ORNL March 24-26. The competition, in its fourth year, attracts students developing new technologies and venture investors with expertise in the market. The first-prize winners each received $25,000. Douglas Hutchings, Stephen Ritterbush, and Seth Shumate from Arkansas won first place in the energy division for Silicon Solar Solutions. "Our method replaces the expensive top layer of solar cells with a thinner, large-grain polysilicon at lower temperatures, which reduces cost and is appealing to manufacturers," said Ritterbush.

300

Freeze plug proves safe, economical in riser repair  

SciTech Connect

In October 1992, Exxon Pipeline Co., Houston, performed in the Gulf of Mexico what the company believes to have been the first underwater freeze-plug procedure. To form a plug, water in a small section of the pipe is frozen with liquid nitrogen. In partially replacing a 10-in. riser at South Marsh Island Block 6A, Exxon Pipeline worked closely with a freeze-plug service company to minimize environmental and personnel exposure and to avoid the chance of an oil spill. The freeze plug reduced the time the pipe was open-ended during the repair, and hydrotesting the freeze plug area and repair section ensured integrity. The paper describes onshore testing of the procedures, pre-work surface cleaning, and the freeze-plug procedure.

Nelson, M.J. [Exxon Co. U.S.A., New Orleans, LA (United States)

1995-05-01T23:59:59.000Z

Note: This page contains sample records for the topic "double-strand break repair" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


301

How does a thermal binary crystal break under shear?  

E-Print Network (OSTI)

When exposed to strong shearing, the particles in a crystal will rearrange and ultimately, the crystal will break by forming large nonaffine defects. Even for the initial stage of this process, only little effort has been devoted to the understanding of the breaking process on the scale of the individual particle size for thermalized mixed crystals. Here, we explore the shear-induced breaking for an equimolar two-dimensional binary model crystal with a high interaction asymmetry between the two different species such that the initial crystal has an intersecting square sublattice of the two constituents. Using Brownian dynamics computer simulations, we show that the combination of shear and thermal fluctuations leads to a characteristic hierarchical breaking scenario where initially, the more strongly coupled particles are thermally distorted, paving the way for the weakly coupled particles to escape from their cage. This in turn leads to mobile defects which may finally merge, proliferating a cascade of defects, which triggers the final breakage of the crystal. This scenario is in marked contrast to the breakage of one-component crystals close to melting. Moreover, we explore the orientational dependence of the initial shear direction relative to the crystal orientation and compare this to the usual melting scenario without shear. Our results are verifiable in real-space experiments of superparamagnetic colloidal mixtures at a pending air-water interface in an external magnetic field where the shear can be induced by an external laser field.

Tobias Horn; Hartmut Löwen

2014-11-21T23:59:59.000Z

302

Vacuum condensates, flavor mixing and spontaneous supersymmetry breaking  

E-Print Network (OSTI)

Spontaneous supersymmetry (SUSY) breaking is revealed in all phenomena in which vacuum condensates are physically relevant. The dynamical breakdown of SUSY is generated by the condensates themselves, which lift the zero point energy. Evidence is presented in the case of the Wess-Zuimino model, and the flavor mixing case is treated in detail.

Antonio Capolupo; Marco Di Mauro

2013-01-05T23:59:59.000Z

303

Dimension-5 operators in a Gauge Mediated Supersymmetry Breaking Model  

E-Print Network (OSTI)

We study the novel features in a model with Gauge Mediated Supersymmetry Breaking. If the messenger fields have positive R-parity, there will be new sources of flavor violations. We show that the dimension-5 operators will be quite important. When dressing these operators by wino-loops, the constraints on them by the present data are given.

Da-Xin Zhang

2004-12-28T23:59:59.000Z

304

Towards breaking temperature equilibrium in multi-component Eulerian schemes  

SciTech Connect

We investigate the effects ofthermal equilibrium on hydrodynamic flows and describe models for breaking the assumption ofa single temperature for a mixture of components in a cell. A computational study comparing pressure-temperature equilibrium simulations of two dimensional implosions with explicit front tracking is described as well as implementation and J-D calculations for non-equilibrium temperature methods.

Grove, John W [Los Alamos National Laboratory; Masser, Thomas [Los Alamos National Laboratory

2009-01-01T23:59:59.000Z

305

Aeration Due to Breaking Waves. Part I: Bubble Populations  

Science Journals Connector (OSTI)

The population of bubbles produced by breaking waves in (10 m) winds of up to 12 m s?1 is analyzed using calibrated images from a vertical pencil-beam sonar system placed on the seabed near the Dutch coast. The structure in the images is ...

A. Graham; D. K. Woolf; A. J. Hall

2004-05-01T23:59:59.000Z

306

Providing protection: Agencies receive funding to repair, upgrade dams  

E-Print Network (OSTI)

Story by Kathy Wythe tx H2O | pg. 26 Providing protection Agencies receive funding to repair, upgrade dams along with local partners, can apply for grant funds, he said. Construction of the dams began through four federal authorizations... totaling about $11 million. Of the 343 dams currently classified as high hazard, Scattered across Texas are almost 2,000 nondescript, earthen dams built on private land to protect property, roads, and bridges from flood damages. Some of these dams...

Wythe, Kathy

2009-01-01T23:59:59.000Z

307

Biological Effects of Space Radiation on Human Cells: History, Advances and Outcomes  

Science Journals Connector (OSTI)

......XIV crossed intense solar flares explaining...and clo- nogenic cell survival assays that...Although the onboard technology available did not...were likely due to solar flares. From all...time variation of solar cosmic rays during...double-strand breaks in human cells: history, progress......

Mira Maalouf; Marco Durante; Nicolas Foray

2011-03-01T23:59:59.000Z

308

Ionizing radiation damage to the folded chromosome of Escherichia coli K-12: sedimentation properties of irradiated nucleoids and chromosomal deoxyribonucleic acid.  

Science Journals Connector (OSTI)

...double-strand breaks by ionizing radiation listed in Table 2 were calculated...4, 5). For this reason, doses of ionizing radiation in excess of 10 krads were used...the biologically significant dose range of less than 10 krads suggested...

K M Ulmer; R F Gomez; A J Sinskey

1979-05-01T23:59:59.000Z

309

Chapter 32 - Cellular Signaling in Dentin Repair and Regeneration  

Science Journals Connector (OSTI)

Abstract Injury to the dentin-pulp complex results in a variety of cellular responses that determine tooth vitality and survival. Initially, these responses center on the odontoblasts as the first line of defense and environmental sensors, but will subsequently encompass other cells in the pulp with a complex interplay being seen between regenerative responses involving odontoblasts and the inflammation associated with pulpal responses to injury. Local degradation of dentin matrix following injury will release bioactive molecules contributing to cellular signaling. While tissue repair and regeneration will likely occur only when infection and inflammation are under control, low-level cytokine stimulation during the earlier stages of dental disease, and possibly as resolution of inflammation ensues, may facilitate and promote reparative events. Whether odontoblasts survive the injurious challenge will determine if reactionary or reparative dentinogenesis occurs during repair and regeneration in the dentin-pulp complex. Notably the complexity of cellular responses is significantly greater for reparative dentinogenesis where stem/progenitor cell recruitment and induction of differentiation are required prior to up-regulation of cellular secretion of new matrix. Angiogeneic and neurogenic signaling are also important events during repair and regeneration. Improved understanding of cellular signaling following tooth injury helps underpin our approaches to diagnosis and treatment planning, as well as driving novel strategies for translation of new clinical therapeutic approaches.

Anthony (Tony) J. Smith; Paul R. Cooper

2015-01-01T23:59:59.000Z

310

Managed operation and repair of a deteriorating large diameter pipeline  

SciTech Connect

Since 1986, Brandon Shores Generating Station has successfully managed a recurring maintenance problem with the buried prestressed concrete cylinder pipe (PCCP) of its circulating water system. It is the intent of this paper to provide a history of the plant`s approach to safely operating and maintaining the deteriorating pipe of this system. This paper will briefly discuss the results of failure analysis performed on the ruptured pipe. Findings of engineering studies addressing the structural integrity of the remaining pipeline will be discussed. The inspection methodology used at Brandon Shores to periodically assess the pipe will be described. Several methods of pipe repair, both external and internal will be detailed. Particular focus will be placed on an internal repair design successfully implemented on two pipe sections during a 1993 plant outage. This internal repair resulted in no loss of hydraulic section, and was erected in-place using conventional materials which were entirely transported through 36 inch manholes. The pipeline was placed back in service and has performed satisfactorily to date.

Cramblitt, K.L. [Baltimore Gas and Electric Company, Baltimore, MD (United States); Lawson, T.J. [Construction Technology Laboratories Inc., Skokie, IL (United States); Ciolko, A.T. [Construction Technology Labs. Inc., Skokie, IL (United States)

1996-07-01T23:59:59.000Z

311

Recombinant Modified Vaccinia Virus Ankara Generating Excess Early Double-Stranded RNA Transiently Activates Protein Kinase R and Triggers Enhanced Innate Immune Responses  

Science Journals Connector (OSTI)

...interferon beta) were purchased from PBL Assay Science (Piscataway, NJ, USA). Murine IFN-alpha...adaptive immunity by the innate immune system. Science 327 :291-295. doi: 10.1126/science.1183021 . 6. Frenz, T , Z Waibler, J Hofmann...

Michael Wolferstätter; Marc Schweneker; Michaela Späth; Susanne Lukassen; Marieken Klingenberg; Kay Brinkmann; Ursula Wielert; Henning Lauterbach; Hubertus Hochrein; Paul Chaplin; Mark Suter; Jürgen Hausmann

2014-10-08T23:59:59.000Z

312

Noncatalytic Ions Direct the RNA-Dependent RNA Polymerase of Bacterial Double-Stranded RNA Virus ?6 from De Novo Initiation to Elongation  

Science Journals Connector (OSTI)

...the interaction between heparin and a battery of mutant enzymes (Fig. 4A). Mutations...like WT) has a significantly lower thermal stability than WT even in the presence...and structure refinement.) Fig. S1 (Thermal denaturation curves.) Fig. S2 (Electron...

Sam Wright; Minna M. Poranen; Dennis H. Bamford; David I. Stuart; Jonathan M. Grimes

2011-12-28T23:59:59.000Z

313

Specific and Efficient Binding of Xeroderma Pigmentosum Complementation Group A to Double-Strand/Single-Strand DNA Junctions with 3‘- and/or 5‘-ssDNA Branches  

Science Journals Connector (OSTI)

11.?Rademakers, S., Volker, M., Hoogstraten, D., Nigg, A. L., Mone, M. J., Van Zeeland, A. A., Hoeijmakers, J. H., Houtsmuller, A. B., and Vermeulen, W. (2003) Xeroderma pigmentosum group A protein loads as a separate factor onto DNA lesions, Mol. ... Rademakers, Suzanne; Volker, Marcel; Hoogstraten, Deborah; Nigg, Alex L.; Mone, Martijn J.; van Zeeland, Albert A.; Hoeijmakers, Jan H. J.; Houtsmuller, Adriaan B.; Vermeulen, Wim ...

Zhengguan Yang; Marina Roginskaya; Laureen C. Colis; Ashis K. Basu; Steven M. Shell; Yiyong Liu; Phillip R. Musich; Constance M. Harris; Thomas M. Harris; Yue Zou

2006-12-19T23:59:59.000Z

314

One "Cool Tool" That's Helping Repair Your DNA | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

"Cool Tool" That's Helping Repair Your DNA "Cool Tool" That's Helping Repair Your DNA One "Cool Tool" That's Helping Repair Your DNA April 18, 2011 - 1:20pm Addthis Charles Rousseaux Charles Rousseaux Senior Writer, Office of Science What are the key facts? Berkeley Lab's Advanced Light Source produces x-ray light that shines a billion times bright than the sun. Scientists discovered the motor that drives the actual repair of the DNA repair machine called MRN, which is tethered to the rest of the machine in a fairly flexible fashion. Further insight into MRN and other DNA repair machines may also give scientists new insights into fighting some cancers as well as other diseases such as cystic fibrosis. With the arrival of spring, many families are dusting off their tools to make some home improvements. It's worth remembering that nature also has

315

Experimental investigation of small-scale breaking waves : flow visualization across the air-water interface  

E-Print Network (OSTI)

The dynamics of breaking waves significantly affect air-sea fluxes of heat, momentum, mass and energy across the ocean interface. Breaking waves also contribute considerable loading to offshore and coastal structures, and ...

McDonald, Angus Kai

2005-01-01T23:59:59.000Z

316

Navier-Stokes simulations of steep breaking water waves with a coupled air-water interface  

E-Print Network (OSTI)

Wave breaking on the ocean surface significantly facilitates the transfer of mass, momentum, heat and energy across the air-sea interface. In the context of the near field flow about a surface ship, the breaking bow wave ...

Hendrickson, Kelli L

2005-01-01T23:59:59.000Z

317

Quantitative imaging of the air-water flow fields formed by unsteady breaking waves  

E-Print Network (OSTI)

An experimental method for simultaneously measuring the velocity fields on the air and water side of unsteady breaking waves is presented. The method is applied to breaking waves to investigate the physics of the air and ...

Belden, Jesse (Jesse Levi)

2009-01-01T23:59:59.000Z

318

Katz A: Breaking the silence on cancer and sexuality: a handbook for healthcare providers  

Science Journals Connector (OSTI)

Although Breaking the Silence does contain correct factual information about the effects ... the book states that it is a “Handbook for Healthcare Providers” and the back cover...Breaking the Silence clearly spea...

Kelly D. Skinner

2008-06-01T23:59:59.000Z

319

Significance of changes in residual stresses and mechanical properties due to SMAW repair of girth welds in linepipe  

SciTech Connect

This program assessed the effects of SMAW repair welding on changes in surface residual stress distribution, fracture toughness and hardness around girth weld joints in linepipe. The following types of repair welds were studied: a part wall repair, a multiple part wall repair and full wall repair. The results were compared with a non-repaired weld sample. It was found that for the weld samples studied in this program, the full wall repair produced the most severe residual stress distribution followed by the multiple and single part wall repairs. The single repair only slightly increased the residual stress distribution when compared to the as-welded condition. Dramatic reductions in toughness were found in the multiple and full repairs due to coarse-grained regions produced during the repair operations. The single part wall repair exhibited an increase in toughness as a result of the addition of a cosmetic capping pass which resulted in greater grain refinement. This suggests that repair procedures utilizing a stringer or temper bead technique may reduce the effect of weld repairs on toughness.

McGaughy, T.; Boyles, L.

1990-10-31T23:59:59.000Z

320

DOE Joint Genome Institute: Breaking down cellulose without blasting  

NLE Websites -- All DOE Office Websites (Extended Search)

4, 2011 4, 2011 Breaking down cellulose without blasting lignin: "Dry rot" genome offers lessons for biofuel pretreatment WALNUT CREEK, Calif.-Feared by realtors and homeowners alike, dry rot due to the fungus Serpula lacrymans causes millions of dollars worth of damage to homes and buildings around the world. This brown rot fungus' capacity to break down the cellulose in wood led to its selection for sequencing by the U.S. Department of Energy (DOE) Joint Genome Institute (JGI) in 2007, with the goal of identifying the enzymes involved in the degradation process and using the information to improve cellulosic biofuels production. Photo: A variant of Serpula lacrymans causes dry rot. (Dave Brown via Flickr/Creative Commons Attribution 2.0) As reported online July 14 in Science Express, an international team of

Note: This page contains sample records for the topic "double-strand break repair" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


321

Breaking the Disk/Halo Degeneracy with Gravitational Lensing  

E-Print Network (OSTI)

The degeneracy between the disk and the dark matter contribution to galaxy rotation curves remains an important uncertainty in our understanding of disk galaxies. Here we discuss a new method for breaking this degeneracy using gravitational lensing by spiral galaxies, and apply this method to the spiral lens B1600+434 as an example. The combined image and lens photometry constraints allow models for B1600+434 with either a nearly singular dark matter halo, or a halo with a sizable core. A maximum disk model is ruled out with high confidence. Further information, such as the circular velocity of this galaxy, will help break the degeneracies. Future studies of spiral galaxy lenses will be able to determine the relative contribution of disk, bulge, and halo to the mass in the inner parts of galaxies.

Ariyeh H. Maller; Luc Simard; Puragra Guhathakurta; Jens Hjorth; Andreas O. Jaunsen; Ricardo A. Flores; Joel R. Primack

1999-10-12T23:59:59.000Z

322

Landscape of Supersymmetry Breaking Vacua in Geometrically Realized Gauge Theories  

E-Print Network (OSTI)

We study vacuum structure of N=1 supersymmetric quiver gauge theories which can be realized geometrically by D brane probes wrapping cycles of local Calabi-Yau three-folds. In particular, we show that the A_2 quiver theory with gauge group U(N_1) \\times U(N_2) with N_1 / 2 landscape of inequivalent meta-stable vacua where supersymmetry is dynamically broken and all the moduli are stabilized. Each vacuum has distinct unbroken gauge symmetry. B-terms generated by the supersymmetry breaking give rise to gaugino masses at one-loop, and we are left with the bosonic pure Yang-Mills theory in the infrared. We also identify the supersymmetric vacua in this model using their infrared free descriptions and show that the decay rates of the supersymmetry breaking vacua into the supersymmetric vacua can be made parametrically small.

Hirosi Ooguri; Yutaka Ookouchi

2006-07-07T23:59:59.000Z

323

Single-Sector Supersymmetry Breaking, Chirality, and Unification  

SciTech Connect

Calculable single-sector models provide an elegant framework for generating the flavor textures via compositeness, breaking supersymmetry, and explaining the electroweak scale. Such models may be realized naturally in supersymmetric QCD with additional gauge singlets (SSQCD), though it remains challenging to construct models without a surfeit of light exotic states where the Standard Model index emerges naturally. We classify possible single-sector models based on Sp confining SSQCD according to their Standard Model index and number of composite messengers. This leads to simple, calculable models that spontaneously break supersymmetry, reproduce the fermion flavor hierarchy, and explain the Standard Model index dynamically with little or no additional matter. At low energies these theories realize a 'more minimal' soft spectrum with direct mediation and a gravitino LSP.

Behbahani, Siavosh R.; /SLAC /Stanford U., Phys. Dept.; Craig, Nathaniel; /Stanford U., Phys. Dept. /Princeton, Inst. Advanced Study /Rutgers U., Piscataway; Torroba, Gonzalo; /SLAC /Stanford U., Phys. Dept.

2011-08-12T23:59:59.000Z

324

Best Management Practice #3: Distribution System Audits, Leak Detection, and Repair  

Energy.gov (U.S. Department of Energy (DOE))

A distribution system audit, leak detection, and repair programs help Federal facilities reduce water losses and make better use of limited water resources.

325

E-Print Network 3.0 - automobile repair shops Sample Search Results  

NLE Websites -- All DOE Office Websites (Extended Search)

Search Powered by Explorit Topic List Advanced Search Sample search results for: automobile repair shops Page: << < 1 2 3 4 5 > >> 1 Program Director: Jim Headlee Phone:...

326

E-Print Network 3.0 - anemia dna repair Sample Search Results  

NLE Websites -- All DOE Office Websites (Extended Search)

Laboratory Workshop Recent Development in DNA Repair Studies" Feb, 2009, NIRS, Chiba, Japan 2... , Matsumoto T, Niwa O. Radiation induction of delayed recombination in ......

327

Using 3D Painting to Build and Repair Parts | GE Global Research  

NLE Websites -- All DOE Office Websites (Extended Search)

a part or add material to repair an existing part. Cold spray is part of GE's expanded additive manufacturing toolkit. nteneh Kebbede, Manager of the Coating and Surface...

328

E-Print Network 3.0 - arthroscopic instability repair Sample...  

NLE Websites -- All DOE Office Websites (Extended Search)

arthroscopically... of arthroscopic rotator cuff repairs: double-row ... Source: Holzbau, Katherine R.S. - Department of Biomedical Engineering, Wake Forest University Collection:...

329

E-Print Network 3.0 - abdominal wall repair Sample Search Results  

NLE Websites -- All DOE Office Websites (Extended Search)

fluid-structure interaction in Summary: of fluid-structure interaction in abdominal aor- tic aneurysms: initial wall stress effects 25 3... of endovascular aneurysm repair (EVAR)....

330

An integrated structural health monitoring approach to composite-based pipeline repair.  

E-Print Network (OSTI)

??One of the most common problems in the structural integrity of industrial pipelines is external corrosion. Composite materials have been accepted as a repair option… (more)

Abd Murad, Mahadi

2011-01-01T23:59:59.000Z

331

Universality-breaking effects in leptonic Z decays  

Science Journals Connector (OSTI)

We analyze the possibility of universality violation in diagonal leptonic decays of the Z boson, in the context of interfamily ‘‘seesaw’’ models. In a minimal extension of the standard model with right-handed neutrino fields, we find that universality-breaking effects increase quadratically with the heavy Majorana neutrino mass and may be observed in the current experiments at the CERN e+e- collider LEP.

J. Bernabéu; J. G. Körner; A. Pilaftsis; K. Schilcher

1993-10-25T23:59:59.000Z

332

Is Privatization Enough? Finding Performance Breaks for UK Power Plants  

E-Print Network (OSTI)

can be retrofitted. Though for NOx (and particular gas-fired stations) these decrease thermal efficiency as they dial down the combustion temperature to reduce the nitrogen intake from the air (Martin et al., 2007). In the early 1990s National... should be positive for CO2 and SO2 but negative for NOx at least for gas-fired stations. 5 Empirical Model Our model has three main characteristics: the generation technology, efficiency, and the structural breaks in efficiency. As usual...

Triebs, Thomas P.; Pollitt, Michael G.

333

13 - Maintenance and repair of gas turbine components  

Science Journals Connector (OSTI)

Abstract: Material selection is a key factor in gas turbine performance and lifecycle cost because it has a central influence in the maintenance of the gas turbine.1 Further, the operation of a gas turbine does result in gas path degradation2 that impacts lifecycle costs and eventually design, manufacture, material choice and maintenance.3 A component repair programme that minimizes maintenance costs and maximizes equipment availability can be instituted to meet or improve lifecycle cost. This chapter presents the key factors influencing the need for maintenance and the choices available.

T. Álvarez Tejedor; R. Singh; P. Pilidis

2013-01-01T23:59:59.000Z

334

Induction and Persistence of Large ?H2AX Foci by High Linear Energy Transfer Radiation in DNA-Dependent protein kinase–Deficient Cells  

SciTech Connect

Purpose: To evaluate the cell response to DNA double-strand breaks induced by low and high linear energy transfer (LET) radiations when the catalytic subunit of DNA-dependent protein kinase (DNA-PKcs), an essential protein of the nonhomologous end-joining repair pathway, lacks kinase activity. Methods and Materials: CHO10B2, a Chinese hamster ovary cell line, and its derived radiosensitive mutant cell line, irs-20, lacking DNA-PKcs activity, were evaluated after 0 to 3 Gy of ?-rays, plateau and Bragg peak protons, and lithium beams by clonogenic assay, and as a measurement of double-strand breaks, phosphorylated H2AX (?H2AX) foci number and size were quantified by immunocytofluorescence. Results: Irs-20 exhibited greater radiosensitivity and a higher amount of ?H2AX foci than CHO10B2 at 6 hours after irradiation for all types of radiations. Remarkably, CHO10B2 and irs-20 maintained their difference in radiosensitivity after high-LET radiation. Six hours after low-LET radiations, irs-20 did not reach basal levels of ?H2AX at high doses, whereas CHO10B2 recovered basal levels for all doses. After high-LET radiation, only CHO10B2 exhibited a reduction in ?H2AX foci, but it never reached basal levels. Persistent foci in irs-20 confirmed a repair deficiency. Interestingly, after 30 minutes of high-LET radiation both cell lines exhibited large foci (size >0.9 ?m{sup 2}) related to the damage nature, whereas at 6 hours irs-20 showed a higher amount of large foci than CHO10B2, with a 7-fold increase at 3 Gy, that could also be associated to radiosensitivity. Conclusions: We demonstrated, for the first time, an association between deficient DNA-PKcs activity and not only high levels of H2AX phosphorylation but also persistence and size increase of ?H2AX foci after high-LET irradiation.

Bracalente, Candelaria; Ibañez, Irene L. [Departamento de Micro y Nanotecnología, Comisión Nacional de Energía Atómica, San Martín, Buenos Aires (Argentina); Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires (Argentina); Molinari, Beatriz [Departamento de Radiobiología, Comisión Nacional de Energía Atómica, San Martín, Buenos Aires (Argentina); Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires (Argentina); Palmieri, Mónica [Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires (Argentina); Kreiner, Andrés [Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires (Argentina); Gerencia de Investigación y Aplicaciones, Comisión Nacional de Energía Atómica, San Martín, Buenos Aires (Argentina); Escuela de Ciencia y Tecnología, Universidad Nacional de San Martín, San Martín, Buenos Aires (Argentina); Valda, Alejandro [Escuela de Ciencia y Tecnología, Universidad Nacional de San Martín, San Martín, Buenos Aires (Argentina); and others

2013-11-15T23:59:59.000Z

335

The roles of APE1, APE2, DNA polymerase ? and mismatch repair in creating S region DNA breaks during antibody class switch  

Science Journals Connector (OSTI)

...of the cell cycle. antibody class switch|DNA recombination...increases antibody affinity. Class switch recombination (CSR...and the DBL cells showed a greater reduction of CSR. Surprisingly...could be deleterious, given the great amount of reactive oxygen species...

2009-01-01T23:59:59.000Z

336

An integrated approach for performance enhancement in automobile repair shops  

Science Journals Connector (OSTI)

Performance management has been a topic of interest for academicians and practitioners. This paper aims at integrating fuzzy logic, data envelopment analysis (DEA) and fuzzy failure mode and effects analysis (fuzzy FMEA) for enhancing the performance in automobile (car) repair shops. A questionnaire with linguistic terms has been used to collect the data related to customer's perceived service quality and the perceptions are quantified using triangular fuzzy numbers. The weights for the service quality parameters considered are gained through analytical hierarchy process (AHP). Fuzzy perceived quality score is then calculated by combining the fuzzy numbers of criteria with the corresponding weights. Then the service performance is measured using DEA by considering both quantitative and qualitative measures. The results from DEA model provide the relative efficiency measure and efficient input/output targets for each repair shop. For further improvement of the service process, fuzzy FMEA is used to prioritise the potential failure modes. Based on the calculated fuzzy risk priority numbers (FRPN) remedial actions can be initiated.

R. Parameshwaran; P.S.S. Srinivasan; M. Punniyamoorthy

2010-01-01T23:59:59.000Z

337

Effective hardware for connection and repair of polyethylene pipelines using ultrasonic modification and heat shrinkage. Part 2. Production bases for molding of epoxy repair couplings with shape memory  

Science Journals Connector (OSTI)

Production bases developed for the molding of epoxy repair couplings with shape memory are described. It is established that maximum deformation of tubular blanks of the epoxy polymers under investigation, whi...

A. E. Kolosov; O. S. Sakharov; V. I. Sivetskii…

2011-07-01T23:59:59.000Z

338

Excision of Tamoxifen-DNA Adducts by the Human Nucleotide Excision Repair System  

Science Journals Connector (OSTI)

...Using the reconstituted human excision repair system and synthetic DNA substrates, we found that the four types of tamoxifen-DNA...Using the reconstituted human excision repair system and synthetic DNA substrates, we found that the four types of tamoxifen-DNA...

Shinya Shibutani; Joyce T. Reardon; Naomi Suzuki; Aziz Sancar

2000-05-15T23:59:59.000Z

339

Bridge Damage and Repair Costs from Hurricane Katrina Jamie Padgett1  

E-Print Network (OSTI)

Bridge Damage and Repair Costs from Hurricane Katrina Jamie Padgett1 ; Reginald DesRoches2 ; Bryant to repair or replace the bridges damaged during the hurricane is estimated at over $1 billion. This paper describes the observed damage patterns to bridges, including damage attributed to storm surge, wind, impact

Padgett, Jamie Ellen

340

Models for Mixed Irradiation with a 'Reciprocal-Time' Pattern of the Repair Function  

Science Journals Connector (OSTI)

......pattern of the repair function (R; Eq. (10)) assuming the...pattern of repair function (R) using the LQ model parameters...P. D., DeLuca, P. M. Jr., Peason, D. W. and Gould...Radiat Res 95: 4556. 3. McNally, N. J., Ronde, J. De......

Shozo Suzuki; Yuri Miura; Shoichi Mizuno; Yoshiya Furusawa

Note: This page contains sample records for the topic "double-strand break repair" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


341

Optimizing Repair Programs for Consistent Query Answering Monica Caniupan and Leopoldo Bertossi  

E-Print Network (OSTI)

, a logic programming system that implements the stable models semantics, in particular. We also analyze be restored minimally by inserting Q(a) or elim- inating S(a). The repair program contains the following 1Optimizing Repair Programs for Consistent Query Answering Monica Caniupan and Leopoldo Bertossi

Bertossi, Leopoldo

342

Lazy Means Smart: Reducing Repair Bandwidth Costs in Erasure-coded Distributed Storage  

E-Print Network (OSTI)

- ditional bandwidth savings. 1. INTRODUCTION Erasure coding schemes, e.g. Reed-Solomon (RS) codes on the example of Reed-Solomon codes, and then explain the repair bandwidth problem. In an RS(n,k) stor- age scheme. 4. EVALUATION METHODOLOGY Evaluating the efficacy of lazy recovery in reducing repair bandwidth

Alvisi, Lorenzo

343

Myelin repair: the role of stem and precursor cells in multiple sclerosis  

Science Journals Connector (OSTI)

...repair compiled by Siddharthan Chandran, Maeve Caldwell and Nick Allen Myelin repair: the role of stem and precursor cells in...cells and the foetal and adult CNS (figure 3). There are merits and disadvantages associated with selecting any human-derived...

2008-01-01T23:59:59.000Z

344

Evaluating Indoor Exposure Modeling Alternatives for LCA: A Case Study in the Vehicle Repair Industry  

Science Journals Connector (OSTI)

Evaluating Indoor Exposure Modeling Alternatives for LCA: A Case Study in the Vehicle Repair Industry ... Alternatives for modeling occupational exposure in LCA are evaluated using experimental monitoring data in the vehicle-repair industry. ... In addition to their use in occupational hygiene, exposure models may also be applied in environmental assessments, such as risk assessment (RA) and life-cycle assessment (LCA). ...

Evangelia Demou; Stefanie Hellweg; Michael P. Wilson; S. Katharine Hammond; Thomas E. McKone

2009-06-25T23:59:59.000Z

345

Identification and Dissection of a Complex DNA Repair Sensitivity Phenotype in Baker's Yeast  

E-Print Network (OSTI)

Identification and Dissection of a Complex DNA Repair Sensitivity Phenotype in Baker's Yeast Ann-assisted bulk segregant analysis, we identified one other locus, MKT1, and a QTL on Chromosome VII that also) Identification and Dissection of a Complex DNA Repair Sensitivity Phenotype in Baker's Yeast. PLoS Genet 4(7): e

Kruglyak, Leonid

346

A Centralized, TreeBased Approach to Network Repair Service for Multicast Streaming Media  

E-Print Network (OSTI)

transmission, and can forward (via unicast) packets to other repair servers that did not receive the packets via the original transmission. After a fixed delay on the order of several seconds, a repair server with a transmission that is delayed by a few seconds relative to the original multicast session. However, this delayed

Maxemchuk, Nicholas F.

347

Genetic Variation in the Nucleotide Excision Repair Pathway and Colorectal Cancer Risk  

Science Journals Connector (OSTI)

...complex formation and stimulation of XPC repair activity. Mol Cell Biol 1997;17:6924-31. 35 van der Spek PJ, Eker A, Rademakers S, et al. XPC and human homologs of RAD23: intracellular localization and relationship to other nucleotide excision repair...

Sonja I. Berndt; Elizabeth A. Platz; M. Daniele Fallin; Lucy W. Thuita; Sandra C. Hoffman; Kathy J. Helzlsouer

2006-11-01T23:59:59.000Z

348

Repair welding influence on offshore pipelines residual stress fields: An experimental study  

Science Journals Connector (OSTI)

Abstract Repair welds, are frequently used in steel structures either to remedy initial fabrication defects, or to rectify in-service degradations of the components. Some previous investigations indicated that repair welding is likely to pose adverse effects on the long-term integrity of the structure exposed to high pressure and temperature actions. It is believed that high residual stresses, associated with the repair process, most probably play an important role in many of subsequent failures. Repair welds might aggravate the size, magnitude and distribution of the tensile residual stresses in the weldments. These adversely affect the component structural integrity and remaining life. So far, no generally accepted guideline is available to provide reliable evaluations on the possible side effects from the repair welding in offshore oil/gas pipelines. This paper reports the result of residual stress measurement on single/double and partial/full repair welds in offshore pipelines. The semi destructive blind hole drilling and destructive sectioning methods have been employed to measure the residual stress fields in each case. In general, the results of the two measurement methods are in reasonable agreement. Residual stresses which are caused by full and partial repairs in the studied samples slightly increased the residual stress distribution when compared to the as-welded condition. Repetition of repair welding in same area influenced the residual stresses' magnitude and distribution especially in areas close to the weld centre line.

M. Zeinoddini; S. Arnavaz; A.P. Zandi; Y. Alizadeh Vaghasloo

2013-01-01T23:59:59.000Z

349

Pipeline repair development in support of the Oman to India gas pipeline  

SciTech Connect

This paper provides a summary of development which has been conducted to date for the ultra deep, diverless pipeline repair system for the proposed Oman to India Gas Pipeline. The work has addressed critical development areas involving testing and/or prototype development of tools and procedures required to perform a diverless pipeline repair in water depths of up to 3,525 m.

Abadie, W.; Carlson, W.

1995-12-01T23:59:59.000Z

350

Rec2 Interplay with both Brh2 and Rad51 Balances Recombinational Repair in Ustilago maydis  

Science Journals Connector (OSTI)

...recombinational repair system powered by Rad51...recombinational repair system. Here, we have...in the vertebrate system, all five Rad51...catalytic muscle to power recombination...sensitivity and restoration of Rad51 focus formation...King (ed.), Handbook of genetics, vol...

Milorad Kojic; Qingwen Zhou; Michael Lisby; William K. Holloman

2006-01-15T23:59:59.000Z

351

Assessing the Risk of Mercury in Drinking Water after UV Lamp Breaks Page 1 Assessing the Risk of Mercury in Drinking Water after UV Lamp Breaks  

E-Print Network (OSTI)

Assessing the Risk of Mercury in Drinking Water after UV Lamp Breaks Page 1 Assessing the Risk of Mercury in Drinking Water after UV Lamp Breaks Heidi Borchers University of New Hampshire, Environmental Ultraviolet (UV) lamps generate ultraviolet light through the vaporization of elemental mercury, by using

352

Repair of gamma-ray-induced DNA base damage in xeroderma pigmentosum cells  

SciTech Connect

The repair of DNA damage produced by /sup 137/Cs gamma irradiation was measured with a preparation from Micrococcus luteus containing DNA damage-specific endonucleases in combination with alkaline elution. The frequency of these endonuclease sensitive sites (ESS) was determined after 54 or 110 Gy of oxic irradiation in normal and xeroderma pigmentosum (XP) fibroblasts from complementation groups A, C, D, and G. Repair was rapid in all cell strains with greater than 50% repair after 1.5 h of repair incubation. At later repair times, 12-17 h, more ESS remained in XP than in normal cells. The frequency of excess ESS in XP cells was approximately 0.04 per 10(9) Da of DNA per Gy which was equivalent to 10% of the initial ESS produced. The removal of ESS was comparable in XP cells with normal radiosensitivity and XP3BR cells which have been reported to be moderately radiosensitive.

Fornace, A.J. Jr.; Dobson, P.P.; Kinsella, T.J.

1986-04-01T23:59:59.000Z

353

Influence of multiple sleeve repairs on the structural integrity of gas pipelines  

Science Journals Connector (OSTI)

This paper addresses the structural integrity of gas pipelines with multiple full-encirclement weld repairs. The scope of the work is to identify and quantify the effects of the number and type of repairs, the distance between them, and the pressurization of the pipe to sleeve gap on the mechanical behaviour of the component. The study includes full-scale experimental testing and finite element modelling. Burst tests were carried out in tracts of pipelines removed from service, including various geometric configurations with and without circumferential girth welds. It is concluded that the reliability of the repairs is strongly influenced by the construction procedures and that interaction effects between successive repairs are not appreciable if the repairs are more than a half pipe diameter apart.

J.L Otegui; A Cisilino; A.E Rivas; M Chapetti; G Soula

2002-01-01T23:59:59.000Z

354

Conserved structural chemistry for incision activity in structurally non-homologous apurinic/apyrimidinic endonuclease APE1 and endonuclease IV DNA repair enzymes.  

E-Print Network (OSTI)

specificity for DNA repair enzyme superfamily. Nat StructIV. Characterization of an enzyme that recognizes oxidativemultifunctional DNA-repair enzyme exonuclease III. Nature

Tsutakawa, Susan E.

2014-01-01T23:59:59.000Z

355

Z(4) model: Criticality and break-collapse method  

Science Journals Connector (OSTI)

Within a real-space renormalization-group (RG) framework, we study the criticality of the Z(4) ferromagnet on the square lattice. The phase diagram (exhibiting ferromagnetic, paramagnetic, and nematiclike phase) recovers all the available exact results, and possibly is of high precision everywhere. In particular, we establish the main asymptotic behaviors (bifurcation and Ising regions). In addition, we develop an operational procedure (break-collapse method) which considerably simplifies the exact calculation of arbitrary Z(4) two-terminal clusters (commonly appearing in RG approaches).

Ananias M. Mariz; Constantino Tsallis; Paulo Fulco

1985-11-01T23:59:59.000Z

356

CMB Anisotropies from Outflows in Lyman Break Galaxies  

E-Print Network (OSTI)

Thomson scattering of the Cosmic Microwave Background (CMB) on moving electrons in the outflows of Lyman Break Galaxies (LBGs) at redshifts 2-8 contributes to the small-scale CMB anisotropies. The net effect produced by each outflow depends on its level of deviation from spherical symmetry, caused either by an anisotropic energy injection from the nuclear starburst or quasar activity, or by an inhomogeneous intergalactic environment. We find that for plausible outflow parameters consistent with spectroscopic observations of LBGs, the induced CMB anisotropies on arcminute scales reach up to $\\sim 1 \\mu$K, comparable to the level produced during the epoch of reionization.

Daniel Babich; Abraham Loeb

2006-09-14T23:59:59.000Z

357

CPT- and Lorentz-symmetry breaking: a review  

E-Print Network (OSTI)

The breakdown of spacetime symmetries has recently been identified as a promising candidate signal for underlying physics, possibly arising through quantum-gravitational effects. This talk gives an overview over various aspects of CPT- and Lorentz-violation research. Particular emphasis is given to the interplay between CPT, Lorentz, and translation symmetry, mechanisms for CPT and Lorentz breaking, and the construction of a low-energy quantum-field description of such effect. This quantum field framework, called the SME, is employed to determine possible phenomenological consequences of CPT and Lorentz violation for neutral-meson interferometry.

Ralf Lehnert

2006-12-28T23:59:59.000Z

358

Spontaneous symmetry breaking and optimization of functional renormalization group  

E-Print Network (OSTI)

The requirement for the absence of spontaneous symmetry breaking in the d=1 dimension has been used to optimize the regulator dependence of functional renormalization group equations in the framework of the sine-Gordon scalar field theory. Results obtained by the optimization of this kind were compared to those of the Litim-Pawlowski and the principle of minimal sensitivity optimization scenarios. The optimal parameters of the compactly supported smooth (CSS) regulator, which recovers all major types of regulators in appropriate limits, have been determined beyond the local potential approximation, and the Litim limit of the CSS was found to be the optimal choice.

I. Nandori; I. G. Marian; V. Bacso

2014-02-27T23:59:59.000Z

359

Repair Capacity for UV Light–Induced DNA Damage Associated with Risk of Nonmelanoma Skin Cancer and Tumor Progression  

Science Journals Connector (OSTI)

...reactivation|DNA repair capacity|epidemiology|skin...increasing levels of solar UV radiation (2-4...studying the DNA repair capacity (DRC) as a marker for...keratosis with cumulative solar ultraviolet exposure...influence the DNA repair capacity of normal and skin cancer-affected...

Li-E Wang; Chunying Li; Sara S. Strom; Leonard H. Goldberg; Abenaa Brewster; Zhaozheng Guo; Yawei Qiao; Gary L. Clayman; J. Jack Lee; Adel K. El-Naggar; Victor G. Prieto; Madeleine Duvic; Scott M. Lippman; Randal S. Weber; Margaret L. Kripke; and Qingyi Wei

2007-11-01T23:59:59.000Z

360

Effects of Chronic Low-Dose Ultraviolet B Radiation on DNA Damage and Repair in Mouse Skin  

Science Journals Connector (OSTI)

...of mammalian skin to solar UVB. We analyzed DNA...significantly less repair capacity than rapidly dividing...the loss of this repair capacity could partially contribute...in excision-repair capacity, and potentiation of...low-dose exposure to solar UVB may result in a significant...

David L. Mitchell; Rüdiger Greinert; Frank R. de Gruijl; Kees L. H. Guikers; Eckhard W. Breitbart; Michelle Byrom; Michelle M. Gallmeier; Megan G. Lowery; and Beate Volkmer

1999-06-15T23:59:59.000Z

Note: This page contains sample records for the topic "double-strand break repair" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


361

Garcinol, a Histone Acetyltransferase Inhibitor, Radiosensitizes Cancer Cells by Inhibiting Non-Homologous End Joining  

SciTech Connect

Purpose: Non-homologous end joining (NHEJ), a major pathway used to repair DNA double-strand breaks (DSBs) generated by ionizing radiation (IR), requires chromatin remodeling at DSB sites through the acetylation of histones by histone acetyltransferases (HATs). However, the effect of compounds with HAT inhibitory activities on the DNA damage response (DDR), including the NHEJ and cell cycle checkpoint, as well as on the radiosensitivity of cancer cells, remains largely unclear. Here, we investigated whether garcinol, a HAT inhibitor found in the rinds of Garcinia indica fruit (called mangosteens), has effects on DDR, and whether it can be used for radiosensitization. Methods and Materials: The following assays were used to examine the effect of garcinol on the inhibition of DSB repair, including the following: a conventional neutral comet assay; a cell-based assay recently developed by us, in which NHEJ repair of DSBs on chromosomal DNA was evaluated; the micrococcal nuclease sensitivity assay; and immunoblotting for autophosphorylation of DNA-dependent protein kinase catalytic subunit (DNA-PKcs). We assessed the effect of garcinol on the cell cycle checkpoint after IR treatment by analyzing the phosphorylation levels of checkpoint kinases CHK1 and CHK2 and histone H3, and by cell cycle profile analysis using flow cytometry. The radiosensitizing effect of garcinol was assessed by a clonogenic survival assay, whereas its effects on apoptosis and senescence were examined by annexin V and senescence-associated {beta}-galactosidase (SA-{beta}-Gal) staining, respectively. Results: We found that garcinol inhibits DSB repair, including NHEJ, without affecting cell cycle checkpoint. Garcinol radiosensitized A549 lung and HeLa cervical carcinoma cells with dose enhancement ratios (at 10% surviving fraction) of 1.6 and 1.5, respectively. Cellular senescence induced by IR was enhanced by garcinol. Conclusion: These results suggest that garcinol is a radiosensitizer that inhibits NHEJ and facilitates senescence without impairing activation of the cell cycle checkpoint.

Oike, Takahiro [Division of Multistep Carcinogenesis, National Cancer Center Research Institute, Chuo-ku, Tokyo (Japan) [Division of Multistep Carcinogenesis, National Cancer Center Research Institute, Chuo-ku, Tokyo (Japan); Division of Genome Biology, National Cancer Center Research Institute, Chuo-ku, Tokyo (Japan); Department of Radiation Oncology, Gunma University Graduate School of Medicine, Maebashi, Gunma (Japan); Ogiwara, Hideaki [Division of Genome Biology, National Cancer Center Research Institute, Chuo-ku, Tokyo (Japan)] [Division of Genome Biology, National Cancer Center Research Institute, Chuo-ku, Tokyo (Japan); Torikai, Kohta [Gunma University Heavy Ion Medical Center, Maebashi, Gunma (Japan)] [Gunma University Heavy Ion Medical Center, Maebashi, Gunma (Japan); Nakano, Takashi [Department of Radiation Oncology, Gunma University Graduate School of Medicine, Maebashi, Gunma (Japan)] [Department of Radiation Oncology, Gunma University Graduate School of Medicine, Maebashi, Gunma (Japan); Yokota, Jun [Division of Multistep Carcinogenesis, National Cancer Center Research Institute, Chuo-ku, Tokyo (Japan)] [Division of Multistep Carcinogenesis, National Cancer Center Research Institute, Chuo-ku, Tokyo (Japan); Kohno, Takashi, E-mail: tkkohno@ncc.go.jp [Division of Genome Biology, National Cancer Center Research Institute, Chuo-ku, Tokyo (Japan)] [Division of Genome Biology, National Cancer Center Research Institute, Chuo-ku, Tokyo (Japan)

2012-11-01T23:59:59.000Z

362

Shelf?break tidally induced environmental influences on acoustic propagation  

Science Journals Connector (OSTI)

Continuous wave propagation in the 100–500 Hz band in littoral regions depends upon both time?dependent oceanography and bathymetry. The environmental influences interact nonlinearly in the acoustical time variation especially since the diurnal tidesurface height changes creates time?dependent total water depth. A submesoscale hydrodynamic model developed by Shen and Evans is used with tidal forcing and a simple shelf?break bathymetry to produce surface height variation and internal wave activity due to internal tide in a stratified ocean environment. A three?dimensional parabolic equation acoustic model is used to acoustically probe this environment at various bearings relative to the shelf break and the resulting internal tidal dynamics. In particular the acoustical results are examined for three?dimensional effects such as horizontal refraction. First the influence of bathymetry alone is shown and then compared to the full environment due to hydrodynamic action. The relative influences will then be compared by various measures such as modal decomposition acoustic energy summed over depth and signal gain degradation. [This research is sponsored by the ONR.

2004-01-01T23:59:59.000Z

363

Flavour symmetry breaking in the kaon parton distribution amplitude  

E-Print Network (OSTI)

We compute the kaon's valence-quark (twist-two parton) distribution amplitude (PDA) by projecting its Poincare'-covariant Bethe-Salpeter wave-function onto the light-front. At a scale \\zeta=2GeV, the PDA is a broad, concave and asymmetric function, whose peak is shifted 12-16% away from its position in QCD's conformal limit. These features are a clear expression of SU(3)-flavour-symmetry breaking. They show that the heavier quark in the kaon carries more of the bound-state's momentum than the lighter quark and also that emergent phenomena in QCD modulate the magnitude of flavour-symmetry breaking: it is markedly smaller than one might expect based on the difference between light-quark current masses. Our results add to a body of evidence which indicates that at any energy scale accessible with existing or foreseeable facilities, a reliable guide to the interpretation of experiment requires the use of such nonperturbatively broadened PDAs in leading-order, leading-twist formulae for hard exclusive processes instead of the asymptotic PDA associated with QCD's conformal limit. We illustrate this via the ratio of kaon and pion electromagnetic form factors: using our nonperturbative PDAs in the appropriate formulae, $F_K/F_\\pi=1.23$ at spacelike-$Q^2=17\\,{\\rm GeV}^2$, which compares satisfactorily with the value of $0.92(5)$ inferred in $e^+ e^-$ annihilation at $s=17\\,{\\rm GeV}^2$.

Chao Shi; Lei Chang; Craig D. Roberts; Sebastian M. Schmidt; Peter C. Tandy; Hong-Shi Zong

2014-06-12T23:59:59.000Z

364

Method of tissue repair using a composite material  

DOE Patents (OSTI)

A composite biocompatible hydrogel material includes a porous polymer matrix, the polymer matrix including a plurality of pores and providing a Young's modulus of at least 10 GPa. A calcium comprising salt is disposed in at least some of the pores. The porous polymer matrix can comprise cellulose, including bacterial cellulose. The composite can be used as a bone graft material. A method of tissue repair within the body of animals includes the steps of providing a composite biocompatible hydrogel material including a porous polymer matrix, the polymer matrix including a plurality of pores and providing a Young's modulus of at least 10 GPa, and inserting the hydrogel material into cartilage or bone tissue of an animal, wherein the hydrogel material supports cell colonization in vitro for autologous cell seeding.

Hutchens, Stacy A; Woodward, Jonathan; Evans, Barbara R; O'Neill, Hugh M

2014-03-18T23:59:59.000Z

365

Experience in the repair of steam generator auxiliary feedwater nozzle  

SciTech Connect

The auxiliary feedwater nozzle is quite often subjected to more thermal stress cycles and other loading mechanisms during their service life than the material was designed and fabricated for at the nozzle of the earlier steam generators in many nuclear plants. During plant operation, the auxiliary feedwater nozzle outlet is exposed to the hot steam from the generator side, while the auxiliary feedwater piping which contains subcooled water from the inlet often induces water hammer as a result of the steam-water mixing phenomena. The thermal cycles and the steam bubble collapse at the nozzle may cause cracking in the nozzle liner and interior surface of the nozzle, and subsequently results in structural damage to the steam generator. This presentation is intended to share the lessons learned from the evaluation of the nozzle condition and the subsequent modification and repair made to the auxiliary feedwater nozzle at the Palisades Nuclear Plant. Other nuclear plant owners may benefit from this experience.

Chao, K.K.N. [Consumers Power Co., Jackson, MI (United States)

1996-12-01T23:59:59.000Z

366

Development of a Remote External Repair Tool for Damaged or Defective Polyethylene Pipe  

SciTech Connect

Current procedures for repairing polyethylene (PE) gas pipe require excavation, isolation, and removal of the damaged section of pipe followed by fusing a new section of pipe into place. These techniques are costly and very disruptive. An alternative repair method was developed at Timberline Tool with support from Oregon State University (OSU) and funding by the U. S. Department of Energy National Energy Technology Laboratory (DOE/NETL). This project was undertaken to design, develop and test a tool and method for repairing damaged PE pipe remotely and externally in situ without squeezing off the flow of gas, eliminating the need for large-scale excavations. Through an iterative design and development approach, a final engineered prototype was developed that utilizes a unique thermo-chemical and mechanical process to apply a permanent external patch to repair small nicks, gouges and punctures under line pressure. The project identified several technical challenges during the design and development process. The repair tool must be capable of being installed under live conditions and operate in an 18-inch keyhole. This would eliminate the need for extensive excavations thus reducing the cost of the repair. Initially, the tool must be able to control the leak by encapsulating the pipe and apply slight pressure at the site of damage. Finally, the repair method must be permanent at typical operating pressures. The overall results of the project have established a permanent external repair method for use on damaged PE gas pipe in a safe and cost-effective manner. The engineered prototype was subjected to comprehensive testing and evaluation to validate the performance. Using the new repair tool, samples of 4-inch PE pipe with simulated damage were successfully repaired under line pressure to the satisfaction of DOE/NETL and the following natural gas companies: Northwest Natural; Sempra Energy, Southwest Gas Corporation, Questar, and Nicor. However, initial results of accelerated age testing on repaired pipe samples showed that the high density polyethylene (HDPE) pipe patch material developed a small crack at the high stress areas surrounding the patched hole within the first 48 hours of hot water testing, indicating that the patch material has a 25-year lifespan. Based on these results, further research is continuing to develop a stronger repair patch for a satisfactory 50-year patch system. Additional tests were also conducted to evaluate whether any of the critical performance properties of the PE pipe were reduced or compromised by the repair technique. This testing validated a satisfactory 50-year patch system for the pipe.

Kenneth H. Green; Willie E. Rochefort; Nick Wannenmacher; John A. Clark; Kevin Harris

2006-06-30T23:59:59.000Z

367

Solvent-mediated repair and patterning of surfaces by AFM  

SciTech Connect

A tip-based approach to shaping surfaces of soluble materials with nanometer-scale control is reported. The proposed method can be used, for example, to eliminate defects and inhomogeneities in surface shape, repair mechanical or laser-induced damage to surfaces, or perform 3D lithography on the length scale of an AFM tip. The phenomenon that enables smoothing and repair of surfaces is based on the transport of material from regions of high- to low-curvature within the solution meniscus formed in a solvent-containing atmosphere between the surface in question and an AFM tip scanned over the surface. Using in situ AFM measurements of the kinetics of surface remodeling on KDP (KH{sub 2}PO{sub 4}) crystals in humid air, we show that redistribution of solute material during relaxation of grooves and mounds is driven by a reduction in surface free energy as described by the Gibbs-Thomson law. We find that the perturbation from a flat interface evolves according to the diffusion equation where the effective diffusivity is determined by the product of the surface stiffness and the step kinetic coefficient. We also show that, surprisingly, if the tip is instead scanned over or kept stationary above an atomically flat area of the surface, a convex structure is formed with a diameter that is controlled by the dimensions of the meniscus, indicating that the presence of the tip and meniscus reduces the substrate chemical potential beneath that of the free surface. This allows one to create nanometer-scale 3D structures of arbitrary shape without the removal of substrate material or the use of extrinsic masks or chemical compounds. Potential applications of these tip-based phenomena are discussed.

Elhadj, S; Chernov, A; De Yoreo, J

2007-10-30T23:59:59.000Z

368

CSNI specialist meeting on leak-before-break in nuclear reactor piping: proceedings  

SciTech Connect

On September 1 and 2, 1983, the CSNI subcommittee on primary system integrity held a special meeting in Monterey, California, on the subject of leak-before-break in nuclear reactor piping systems. The purpose of the meeting was to provide an international forum for the exchange of ideas, positions, and research results; to identify areas requiring additional research and development; and to determine the general attitude toward acceptance of the leak-before-break concept. The importance of the leak-before-break issue was evidenced by excellent attendance at the meeting and through active participation by the meeting attendees. Approximately 125 people representing fifteen different nations attended the meeting. The meeting was divided into four technical sessions addressing the following areas: Application of Piping Fracture Mechanics to Leak-Before Break, Leak Rate and Leak Detection, Leak-Before-Break Studies, Methods and Results, Current and Proposed Positions on Leak-Before-Break.

Not Available

1984-08-01T23:59:59.000Z

369

Fracture behavior of surface cracked wide plates of high strength steel containing overmatched repair welds  

SciTech Connect

This paper presents the experimental results of tests conducted using surface cracked wide plates containing overmatched repair weld joints. The deformation and fracture characteristics of the repair welded wideplates notched at the original weld deposit, repair weld and HAZ regions are discussed. The aim of this work was to investigate the effects of strength mis-match and notch position on the fracture performance of such complex weldments. Furthermore, the predictions of crack driving force using the Engineering Treatment Model for mis-matched welds (ETM-MM) procedure was compared with the results of the wide plates containing semielliptical surface cracks. For this study, 1/2K weld joints were prepared on 30 nm thick pipeline steel X65 plates by using a SAW process, resulting in 50% overmatching. Repair was performed at the cap side of the original joint up to half depth of plate thickness with a GMA welding process under hyperbaric conditions, leading to 41% yield strength overmatching. In order to assess the fracture behavior of these welds, surface cracked (semielliptic defects) wide plates containing original and repair welds were tested in tension at {minus}10 C. The surface cracked wide plate tests results have confirmed that overmatched repair weld metal can exert a significant effect on the deformation and fracture behavior of the wide plates. Wide plates containing root cracks clearly showed a shielding effect of the overmatched repair weld since it prevented development of through thickness ligament yielding.

Junghans, E.; Kocak, M.; Schwalbe, K.H. [GKSS Research Center, Geesthacht (Germany). Inst. of Materials Research

1996-12-01T23:59:59.000Z

370

Effect of multiple repairs in girth welds of pipelines on the mechanical properties  

SciTech Connect

This work presents the results of multiple weld repairs in the same area in seamless API X-52 microalloyed steel pipe. Four conditions of shielded metal arc welding repairs and one as-welded specimen of the girth weld were characterized to determine changes in the microstructure, grain size in the heat affected zone, and to evaluate their effect on the mechanical properties of the weld joints. The mechanical properties by means of tension tests, Charpy-V impact resistance and Vickers hardness of the welds were analyzed. The results indicate that significant changes are not generated in the microstructural constituents of the heat affected zone. Grain growth in the heat affected zone at the specimen mid-thickness with the number of repairs was observed. Tensile strength of the weld joints meets the requirement of the API 1104 standard even after the fourth weld repair. Significant reduction in Charpy-V impact resistance with the number of weld repairs was found when the notch location was in the intersection of the fusion line with the specimen mid-thickness. A significant increase in the Vickers hardness of the heat affected zone occurred after the first repair and a gradual decrease in the Vickers hardness occurred as the number of repairs increases.

Vega, O.E.; Hallen, J.M. [Departamento de Ingenieria Metalurgica, ESIQIE-IPN, Laboratorios Pesados de Metalurgia, UPALM, Zacatenco, C.P. 07738, Mexico D.F. (Mexico); Villagomez, A. [Construcciones Maritimas Mexicanas, CMM-PROTEXA, Av. Periferica s/n, Fracc. Lomas de Holche, C.P. 24120, Cd. del Carmen, Campeche (Mexico); Contreras, A. [Instituto Mexicano del Petroleo, Investigacion en Ductos, Corrosion y Materiales, Eje Central Lazaro Cardenas Norte 152 Col. San Bartolo Atepehuacan, C.P. 07730, Mexico D.F. (Mexico)], E-mail: acontrer@imp.mx

2008-10-15T23:59:59.000Z

371

Fabrication Flaw Density and Distribution In Repairs to Reactor Pressure Vessel and Piping Welds  

SciTech Connect

The Pacific Northwest National Laboratory is developing a generalized fabrication flaw distribution for the population of nuclear reactor pressure vessels and for piping welds in U.S. operating reactors. The purpose of the generalized flaw distribution is to predict component-specific flaw densities. The estimates of fabrication flaws are intended for use in fracture mechanics structural integrity assessments. Structural integrity assessments, such as estimating the frequency of loss-of-coolant accidents, are performed by computer codes that require, as input, accurate estimates of flaw densities. Welds from four different reactor pressure vessels and a collection of archived pipes have been studied to develop empirical estimates of fabrication flaw densities. This report describes the fabrication flaw distribution and characterization in the repair weld metal of vessels and piping. This work indicates that large flaws occur in these repairs. These results show that repair flaws are complex in composition and sometimes include cracks on the ends of the repair cavities. Parametric analysis using an exponential fit is performed on the data. The relevance of construction records is established for describing fabrication processes and product forms. An analysis of these records shows there was a significant change in repair frequency over the years when these components were fabricated. A description of repair flaw morphology is provided with a discussion of fracture mechanics significance. Fabrication flaws in repairs are characterized using optimized-access, high-sensitivity nondestructive ultrasonic testing. Flaw characterizations are then validated by other nondestructive evaluation techniques and complemented by destructive testing.

GJ Schuster, FA Simonen, SR Doctor

2008-04-01T23:59:59.000Z

372

Comparative Study of Inguinal Hernia Repair Rates After Radical Prostatectomy or External Beam Radiotherapy  

SciTech Connect

Purpose: We tested the hypothesis that patients treated for localized prostate cancer with radical prostatectomy (RP) have a higher risk of requiring an inguinal hernia (IH) repair than their counterparts treated with external beam radiotherapy (EBRT). Methods and Materials: Within the Quebec Health Plan database, we identified 6,422 men treated with RP and 4,685 men treated with EBRT for localized prostate cancer between 1990 and 2000, in addition to 6,933 control patients who underwent a prostate biopsy. From among that population, we identified patients who underwent a unilateral or bilateral hernia repair after either RP or EBRT. Kaplan-Meier plots showed IH repair-free survival rates. Univariable and multivariable Cox regression models tested the predictors of IH repair after RP or EBRT. Covariates consisted of age, year of surgery, and Charlson Comorbidity Index. Results: IH repair-free survival rates at 1, 2, 5, and 10 years were 96.8, 94.3, 90.5, and 86.2% vs. 98.9, 98.0, 95.4, and 92.2%, respectively, in RP vs. EBRT patients (log-rank test, p < 0.001). IH repair-free survival rates in the biopsy population were 98.3, 97.1, 94.9, and 90.2% at the same four time points. In multivariable Cox regression models, RP predisposed to a 2.3-fold higher risk of IH repair than EBRT (p < 0.001). Besides therapy type, patient age (p < 0.001) represented the only other independent predictor of IH repair. Conclusions: RP predisposes to a higher rate of IH repair relative to EBRT. This observation should be considered at informed consent.

Lughezzani, Giovanni [Cancer Prognostics and Health Outcomes Unit, University of Montreal Health Center, Montreal, Quebec (Canada); Department of Urology, Vita-Salute San Raffaele University, Milan (Italy); Sun, Maxine [Cancer Prognostics and Health Outcomes Unit, University of Montreal Health Center, Montreal, Quebec (Canada); Perrotte, Paul [Department of Urology, University of Montreal, Montreal, Quebec (Canada); Alasker, Ahmed; Jeldres, Claudio [Cancer Prognostics and Health Outcomes Unit, University of Montreal Health Center, Montreal, Quebec (Canada); Isbarn, Hendrik; Budaeus, Lars [Cancer Prognostics and Health Outcomes Unit, University of Montreal Health Center, Montreal, Quebec (Canada); Martini-clinic, Prostate Cancer Center Hamburg-Eppendorf, Hamburg (Germany); Lattouf, Jean-Baptiste; Valiquette, Luc; Benard, Francois; Saad, Fred [Department of Urology, University of Montreal, Montreal, Quebec (Canada); Graefen, Markus [Cancer Prognostics and Health Outcomes Unit, University of Montreal Health Center, Montreal, Quebec (Canada); Martini-clinic, Prostate Cancer Center Hamburg-Eppendorf, Hamburg (Germany); Montorsi, Francesco [Department of Urology, Vita-Salute San Raffaele University, Milan (Italy); Karakiewicz, Pierre I., E-mail: pierre.karakiewicz@umontreal.c [Cancer Prognostics and Health Outcomes Unit, University of Montreal Health Center, Montreal, Quebec (Canada)

2010-12-01T23:59:59.000Z

373

ON THE EVOLUTION OF THE SPECTRAL BREAK IN THE AFTERGLOW OF GAMMA-RAY BURSTS  

SciTech Connect

The temporal evolution of the spectral break in the time-resolved spectral energy density of the broadband afterglow of gamma-ray bursts (GRBs) 091127 and 080319B was shown recently to be inconsistent with that expected for the cooling break in the standard fireball model of GRBs. Here we show that it is, however, in good agreement with the predicted temporal evolution of the smooth injection break/bend in the cannonball model of GRBs.

Dado, Shlomo; Dar, Arnon [Physics Department, Technion, Haifa 32000 (Israel)

2012-02-20T23:59:59.000Z

374

Maintenance and repair expenditures: determinants and tradeoffs with new capital goods  

Science Journals Connector (OSTI)

Traditionally economic analysis has focused on new capital investment, while ignoring expenditures to maintain or repair existing stock. Recent, broader theories of replacement have emphasized the important role played by both maintenance and utilization rates. However, the decision to extend the service life of capital has been given scant empirical attention, owing partly to a lack of appropriate data. This study exploits survey data that report annual maintenance/repair expenditures for Canadian manufacturing industries. A standard production framework, modified to incorporate endogenous depreciation, guides the econometric analysis. The empirical results indicate that the cost of capital, among other variables, has a statistically significant effect on maintenance/repair decisions.

J.K. Mullen; Martin Williams

2004-01-01T23:59:59.000Z

375

Assessment of Concrete Repair Techniques for Radiologically Contaminated Tank Farm Pump and Valve Pits  

SciTech Connect

As part of the scope of Project W-314, ''Tank Farm Restoration and Safe Operations,'' the condition of pump and valve pit walls and floors is being assessed, and repairs made as needed, to support upgrading the infrastructure necessary to safely transfer tank waste for treatment. Flaws in the surfaces of the pits (e.g., concrete crack/faults, protective coating deterioration) must be repaired to ensure containment integrity and to facilitate future decontamination of the pits. This engineering study presents a cost/risk/benefit evaluation of concrete and protective coating repair methods in pump and valve pits using various manual and remote tool systems.

MINTEER, D.J.

2000-09-19T23:59:59.000Z

376

An alternative methodology to repair localized corrosion damage in metallic pipelines with epoxy resins  

Science Journals Connector (OSTI)

The present work is concerned with the analysis of epoxy repair systems for metallic pipelines undergoing elastic or inelastic deformations with localized corrosion damage that impair the serviceability. In the case of trough-thickness damage, the main focus is to assure an adequate application of the epoxy filler in such a way the pipe wont leak after repair. Such a procedure can be used or not associated with a composite sleeve that assures a satisfactory level of structural integrity. Examples concerning the use of repair systems in different damage situations are presented and analyzed showing the possibilities of practical use of the proposed methodology.

H.S. da Costa-Mattos; J.M.L. Reis; R.F. Sampaio; V.A. Perrut

2009-01-01T23:59:59.000Z

377

Microsoft Word - SPR Emergency Pipeline Repair, 2013-2017 NEPA.docx  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Emergency Pipeline and Piping Repair, 2013-2017 Emergency Pipeline and Piping Repair, 2013-2017 Description: Subcontractor shall shall provide all labor, materials, equipment, supervision, transportation and services required to perform emergency repairs on the SPR system site piping (including underground fire protection systems and offsite pipelines). Subcontractor shall mobilize equipment to perform excavation, backfill, hydrostatic testing, welding and cutting, blasting, coating and painting of large and small diameter piping systems on the SPR facilities and offsite pipelines. Subcontractor shall also assist in emergency exercises by assemblying temporary piping spools and positioning pump skids and temporary supports. This NEPA document covers the period from 2013-2017.

378

Metallic glass could make your next cell phone harder to break  

NLE Websites -- All DOE Office Websites (Extended Search)

Metallic glass could make your next cell phone harder to break Alumni Link: Opportunities, News and Resources for Former Employees Latest Issue:January 2015 All Issues submit...

379

Metallic glass could make your next cell phone harder to break  

NLE Websites -- All DOE Office Websites (Extended Search)

April 2014 Metallic glass could make your next cell phone harder to break Community Connections: Your link to news and opportunities from Los Alamos National Laboratory Latest...

380

Bubbles Help Break Energy Storage Record for Lithium Air-Batteries  

NLE Websites -- All DOE Office Websites (Extended Search)

Bubbles Help Break Energy Storage Record for Lithium Air-Batteries Foam-base graphene keeps oxygen flowing in batteries that holds promise for electric vehicles January...

Note: This page contains sample records for the topic "double-strand break repair" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


381

Planetary Wave Breaking and Nonlinear Reflection: Seasonal Cycle and Interannual Variability  

E-Print Network (OSTI)

and L. M. Polvani, 2000: Climatology of intrusions into theM. H. Hitchman, 1999: A climatology of Rossby wave breaking

Abatzoglou, John T; Magnusdottir, Gudrun

2006-01-01T23:59:59.000Z

382

When Size Matters: Yttrium Oxide Breaking Down Under Pressure | Advanced  

NLE Websites -- All DOE Office Websites (Extended Search)

Breakthrough in Nanocrystals' Growth Breakthrough in Nanocrystals' Growth Next Step to Drought-Resistant Plants? A Boring Material "Stretched" Could Lead to an Electronics Revolution At the Crossroads of Chromosomes Unveiling the Structure of Adenovirus Science Highlights Archives: 2013 | 2012 | 2011 | 2010 2009 | 2008 | 2007 | 2006 2005 | 2004 | 2003 | 2002 2001 | 2000 | 1998 | Subscribe to APS Science Highlights rss feed When Size Matters: Yttrium Oxide Breaking Down Under Pressure NOVEMBER 2, 2010 Bookmark and Share Top: Pair distribution function (PDF) of 16 nm-sized Y2O3 at high pressures. Bottom: Lin Wang (left) and Wenge Yang (right) of the Carnegie Institution of Washington shown with the diamond anvil cell and x-ray instrumentation used to probe the PDF of the sample at high applied

383

Breaking Records in Neurological Microradiology | Advanced Photon Source  

NLE Websites -- All DOE Office Websites (Extended Search)

Exposing Valence-Bond Model Inadequacies Exposing Valence-Bond Model Inadequacies Plants' Rapid Response System Revealed Rewriting the Organofluorine Playbook Computer-Designed Proteins to Disarm a Variety of Flu Viruses Driving Membrane Curvature Science Highlights Archives: 2013 | 2012 | 2011 | 2010 2009 | 2008 | 2007 | 2006 2005 | 2004 | 2003 | 2002 2001 | 2000 | 1998 | Subscribe to APS Science Highlights rss feed Breaking Records in Neurological Microradiology AUGUST 9, 2012 Bookmark and Share A dense neuron cluster including details of cells in deeper tissue layers. As neuroscientists probe ever deeper into the mysteries of the brain and nervous system, they need ever sharper vision. A group of researchers has developed some exciting new techniques for imaging neuronal and synaptic networks using the hard synchrotron x-rays

384

LANL breaks ground on key sediment control project  

NLE Websites -- All DOE Office Websites (Extended Search)

Sediment control project Sediment control project LANL breaks ground on key sediment control project Called "grade-control" structures, the approximately $2 million features are up to eight feet high and made of rocks packed tightly into wire enclosures. November 5, 2009 Los Alamos National Laboratory sits on top of a once-remote mesa in northern New Mexico with the Jemez mountains as a backdrop to research and innovation covering multi-disciplines from bioscience, sustainable energy sources, to plasma physics and new materials. Los Alamos National Laboratory sits on top of a once-remote mesa in northern New Mexico with the Jemez mountains as a backdrop to research and innovation covering multi-disciplines from bioscience, sustainable energy sources, to plasma physics and new materials.

385

Domain Walls and Vortices in Chiral Symmetry Breaking  

E-Print Network (OSTI)

We study domain walls and vortices in chiral symmetry breaking in a QCD-like theory with N flavors in the chiral limit. If the axial anomaly is absent, there exist stable Abelian axial vortices winding around the spontaneously broken U(1)_A symmetry and non-Abelian axial vortices winding around both the U(1)_A and non-Abelian SU(N) chiral symmetries. In the presence of the axial anomaly term, metastable domain walls are present and Abelian axial vortices must be attached by N domain walls, forming domain wall junctions. We show that a domain wall junction decays into N non-Abelian vortices attached by domain walls, implying its metastability. We also show that domain walls decay through the quantum tunneling by creating a hole bounded by a closed non-Abelian vortex.

Minoru Eto; Yuji Hirono; Muneto Nitta

2013-09-18T23:59:59.000Z

386

Embedded defects and symmetry breaking in flipped SU(5)  

Science Journals Connector (OSTI)

We explicitly show the analogy between the symmetry-breaking scheme for the GUT flipped SU(5) with that of the Weinberg-Salam theory of electroweak interactions. This allows us to construct the embedded defect spectrum of the flipped SU(5) theory. We find that the spectrum consists of twelve gauge-equivalent unstable leptoquark strings, which are analogous to W strings in electroweak theory, and another string that is gauge inequivalent to the leptoquark strings which we call the ‘‘V string.’’ The V string is analogous to the Z string of electroweak theory, correspondingly admitting a stable semilocal limit. Using data on the running coupling constants we indicate that in the nonsupersymmetric case V strings can be stable for part of the physically viable parameter space. Cosmological consequences are briefly discussed. © 1995 The American Physical Society.

Anne-Christine Davis and Nathan F. Lepora

1995-12-15T23:59:59.000Z

387

Are There Local Analogs of Lyman Break Galaxies?  

E-Print Network (OSTI)

To make direct comparisons in the rest-far-ultraviolet between LBGs at z~3 and more local star-forming galaxies, we use HST/STIS to image a set of 12 nearby (z<0.05) HII galaxies in the FUV and a set of 14 luminous compact blue galaxies (LCBGs) at moderate redshift (z~0.5) in the NUV, corresponding to the rest-FUV. We then subject both sets of galaxy images and those of LBGs at z~3 to the same morphological and structural analysis. We find many qualitative and quantitative similarities between the rest-FUV characteristics of distant LBGs and of the more nearby starburst samples, including general morphologies, sizes, asymmetries, and concentrations. Along with some kinematic similarities, this implies that nearby HII galaxies and LCBGs may be reasonable local analogs of distant Lyman break galaxies.

James D. Lowenthal; R. Nick Durham; Brian J. Lyons; Matthew A. Bershady; Jesus Gallego; Rafael Guzman; David C. Koo

2005-02-14T23:59:59.000Z

388

A Protein that Repairs Damage to Cancer Cells | Advanced Photon Source  

NLE Websites -- All DOE Office Websites (Extended Search)

An X-ray Vortex on the Horizon? An X-ray Vortex on the Horizon? How Two Drops Become One Scientists Discover How Nanocluster Contaminants Increase Risk of Spreading Mobile RNA is Poised and Ready Glass Does a Double-Take Science Highlights Archives: 2013 | 2012 | 2011 | 2010 2009 | 2008 | 2007 | 2006 2005 | 2004 | 2003 | 2002 2001 | 2000 | 1998 | Subscribe to APS Science Highlights rss feed A Protein that Repairs Damage to Cancer Cells MAY 5, 2008 Bookmark and Share The ABH2-DNA complex. A team of University of Chicago scientists has shown how two proteins locate and repair damaged genetic material inside cells. Because one of the proteins detects and repairs DNA damage that may result from a certain type of cancer therapy, the researchers raised the possibility of designing a molecule that could interfere with the repair process, making cancer

389

“Synthetic Lethality”: Molecular Co-targeting to Restore the DNA Repair Mechanisms in Prostate Cancer Cells  

Science Journals Connector (OSTI)

To this end, PARP inhibitors are the well-known class of drugs that have recently been proposed to reach synthetic lethality in DNA repair-defective, radio-resistant prostate tumors.

Gennaro Ilardi; Stefania Staibano

2013-01-01T23:59:59.000Z

390

Evaluation of versions of electric power grid repair schemes from the results of structural reliability calculations  

Science Journals Connector (OSTI)

A procedure for processing repair claims using the results of an estimate of the structural reliability of the electric grid, the results of the approval of algorithms using the example of a test model, and th...

P. V. Toshchakov; O. M. Kotov; A. F. Kostarev

2013-01-01T23:59:59.000Z

391

Genetic Variation in Base Excision Repair Genes and the Prevalence of Advanced Colorectal Adenoma  

Science Journals Connector (OSTI)

...with dietary factors were adjusted for energy. Statistical analysis. Conditional logistic...Genetic variants that increased repair efficiency may protect against colorectal neoplasia...early-onset colorectal carcinoma in Egypt. Cancer Lett 2000;159:79-86. 45...

Sonja I. Berndt; Wen-Yi Huang; M. Daniele Fallin; Kathy J. Helzlsouer; Elizabeth A. Platz; Joel L. Weissfeld; Timothy R. Church; Robert Welch; Stephen J. Chanock; and Richard B. Hayes

2007-02-01T23:59:59.000Z

392

Repair of Radiation Damage to Deoxyribonucleic Acid in Germinating Spores of Bacillus subtilis  

Science Journals Connector (OSTI)

...Metabolism Repair of Radiation Damage to Deoxyribonucleic...Tsukijii, Tokyo, Japan 1 Present address...in vegetative cells. Radiation-induced single-strand...Tsukijii, Tokyo, Japan Received for publication...in vegetative cells. Radiation-induced single-strand...

Hiroshi Terano; Hiroshi Tanooka; Hajime Kadota

1971-06-01T23:59:59.000Z

393

E-Print Network 3.0 - arthroscopic partial repair Sample Search...  

NLE Websites -- All DOE Office Websites (Extended Search)

Tissue: A Validated Finite Element Model Summary: :364-373, 2005. 2 Arciero, R. A., J. H. Wheeler, J. B. Ryan, and J. T. McBride. Arthroscopic Bankart repair... ., and A. B....

394

MyoD Gene Transfer for Cardiac Repair 2209 J. Clin. Invest.  

E-Print Network (OSTI)

the myogenic determination gene, MyoD, into cardiac granulation (wound repair) tissue. A replica- tion-defective suffer myocardial infarctions have significantly impaired car- diac function. Thus, therapy to induce

Kay, Mark A.

395

Polymorphisms in DNA Repair Genes, Medical Exposure to Ionizing Radiation, and Breast Cancer Risk  

Science Journals Connector (OSTI)

...the role of DSB repair in response to low-dose radiation, particularly the range experienced by women from mammograms and other...breast cancer associated with exposure to low doses of ionizing radiation. Although we specifically designed our questionnaire...

Robert C. Millikan; Jon S. Player; Allan Rene deCotret; Chiu-Kit Tse; and Temitope Keku

2005-10-01T23:59:59.000Z

396

Use of CFRP Overlays to Repair Fatigue Damage in Steel Bridge Girders and Components  

E-Print Network (OSTI)

Fatigue damage in steel girder bridges built prior to the mid-1980s is common due to distortion-induced fatigue. Different repair techniques have been developed and implemented to retrofit bridges of this era with existing fatigue damage...

Gangel, Regan Elisabeth

2012-05-31T23:59:59.000Z

397

USE OF COMPOSITE MATERIALS TO REPAIR STEEL STRUCTURES VULNERABLE TO FATIGUE DAMAGE  

E-Print Network (OSTI)

This study investigates mainly two different topics related to the use of CFRP's for strengthening and repair of steel bridges: the use of CFRP's to prevent damage in fatigue vulnerable welded connections prior to fatigue-crack initiation...

Alemdar, Fatih

2010-01-01T23:59:59.000Z

398

Project: UAF Utilities Waste Line Repairs Ch6 to Ch13 Project No: 2013101 UTWH  

E-Print Network (OSTI)

Project: UAF Utilities Waste Line Repairs Ch6 to Ch13 Project No: 2013101 UTWH Subject: Project Schedule Project Duration: May 27 to August 10, 2014 The sewer line will be constructed in phases

Ickert-Bond, Steffi

399

E-Print Network 3.0 - aortic repair pushing Sample Search Results  

NLE Websites -- All DOE Office Websites (Extended Search)

for: aortic repair pushing Page: << < 1 2 3 4 5 > >> 1 Mechanobiology of the Aortic Heart Valve Jonathan T. Butcher1 Summary: ;13:798-803 18. Deck JD, Thubrikar MJ, Schneider...

400

Application of adhesive materials for the repair of acting gas pipelines  

Science Journals Connector (OSTI)

The materials and equipment developed to eliminate damage in the pipelines that transmit hydro-carbons are presented. The ... adhesive materials and processing methods used in the repairs are described.

V. S. Smirnov; N. N. Parakhina; A. F. Murokh…

2010-04-01T23:59:59.000Z

Note: This page contains sample records for the topic "double-strand break repair" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


401

Fatigue crack retardation by the application of repair coatings to gas pipelines under pressure  

Science Journals Connector (OSTI)

We describe additional capabilities of the repair insulation coatings applied to main pipelines (without taking them out of operation) in increasing the durability of these pipelines. As a specific feature of thi...

T. P. Venhrynyuk

2013-05-01T23:59:59.000Z

402

Study on Sub-sea Pipelines Hyperbaric Welding Repair under High Air Pressures  

Science Journals Connector (OSTI)

Most Chinese sub-sea pipelines are buried in Bohai Sea less than ... are widely used For the application of offshore pipelines repair, the hyperbaric TIG welding process under high...

Canfeng Zhou; Xiangdong Jiao; Long Xue…

2011-01-01T23:59:59.000Z

403

Unmanned deepwater-line repair system passes full-scale trials  

SciTech Connect

The first ever full-scale tests of an unmanned, deepwater-pipeline repair system were successfully conducted last year off the coast of Italy. The Italian gas-transmission company SNAM tested a submersible automatic system (SAS) sealine repair system at a depth of 600 m. The modular SAS allows sealines to be repaired by installation of the Nuovo Pignone mechanical connector. The system's trials simulated complete repair intervention on the 20-in. Trans mediterranean pipeline and provided unprecedented experience to SNAM and to the other involved in this project. The paper discusses the origin of the idea for the SAS, the design of the system, construction and testing, the first sea trials, final deep sea trials, and future developments.

Venzi, S.; Vienna, A. (SNAM SpA, Milan (Italy))

1993-09-06T23:59:59.000Z

404

Tiny Terminators: New Micro-Robots Assemble, Repair Themselves and Are Surprisingly Strong  

Energy.gov (U.S. Department of Energy (DOE))

Tiny robots that can assemble and repair themselves? Watch a video of Argonne National Laboratory's micro-robots that easily perform tasks that can challenge other robots, including moving objects that are larger than themselves.

405

Diverless pipeline-repair system passes tests for 20-in. pipe  

SciTech Connect

Full-scale tests of a diverless pipeline repair system have shown its effectiveness for repairing subsea pipelines of up to 20 in. in diameter. The repair principle is based upon a metal-to-metal seal. The pipe end is cold forged to form a flare. The metal-to-metal seal is located on a cone which is part of the mechanical hydraulically actuated connector. The cone is advanced and the metal-to-metal seal is compressed between the cone and the inside diameter of the flare, forming a high integrity pipeline seal. A set of remotely controlled modules was constructed to perform the repair operations, including pipe flaring. A series of onshore and shallow water trials have demonstrated the practicability of these modules and the feasibility of the new tie-in concept for a diverless pipeline repair. A second set of tools has also been designed for pipeline tie-ins which works with the pipeline-repair spread.

Lerique, M.; Thiberge, P. (Elf Aquitaine, Pau (FR)); Wright, N. (British Gas PLC, Newcastle upon Tyne (UK))

1990-05-21T23:59:59.000Z

406

Design Considerations for Monopile Founded Offshore Wind Turbines Subject to Breaking Waves  

E-Print Network (OSTI)

DESIGN CONSIDERATIONS FOR MONOPILE FOUNDED OFFSHORE WIND TURBINES SUBJECT TO BREAKING WAVES A Thesis by GARRETT REESE OWENS Submitted to the Office of Graduate Studies of Texas A&M University in partial fulfillment... Farms ...... 4 Figure 2 Overview of Offshore Wind Turbine Terminology................................. 7 Figure 3 Overturning Moment as a Function of Water Depth ............................... 10 Figure 4 Types of Breaking Waves...

Owens, Garrett Reese 1987-

2012-11-26T23:59:59.000Z

407

Formation of beads-on-a-string structures during break-up of viscoelastic filaments  

E-Print Network (OSTI)

Break-up of viscoelastic filaments is pervasive in both nature and technology. If a filament is formed by placing a drop of saliva between a thumb and forefinger and is stretched, the filament’s morphology close to break-up ...

Bhat, Pardeep P.

408

Symmetry Breaking in Graphene Gregory S. Boebinger, National High Magnetic Field Laboratory  

E-Print Network (OSTI)

Symmetry Breaking in Graphene Gregory S. Boebinger, National High Magnetic Field Laboratory DMR. In this experiment, Landau levels in graphene were used to study the breaking of SU(4) symmetry--a higher dimensional states by their spin polarization. It was found that graphene turns into either a spin ferromagnet

Weston, Ken

409

Hydrogen bond breaking probed with multidimensional stimulated vibrational echo correlation spectroscopy  

E-Print Network (OSTI)

Hydrogen bond breaking probed with multidimensional stimulated vibrational echo correlation September 2003 Hydrogen bond population dynamics are extricated with exceptional detail using ultrafast ( 50 of methanol­OD oligomers in CCl4 . Hydrogen bond breaking makes it possible to acquire data for times much

Fayer, Michael D.

410

Giving up Twitter for Lent: how and why we take breaks from social media  

Science Journals Connector (OSTI)

Social media use is widespread, but many people worry about overuse. This paper explores how and why people take breaks from social media. Using a mixed methods approach, we pair data from users who tweeted about giving up Twitter for Lent with an interview ... Keywords: Twitter, breaks, internet, media refusal, self-control, social media, willpower

Sarita Yardi Schoenebeck

2014-04-01T23:59:59.000Z

411

On Wave Breaking and the Equilibrium Spectrum of Wind-Generated Waves  

Science Journals Connector (OSTI)

...Equilibrium Spectrum of Wind-Generated Waves M...is made of the loss of energy by wave breaking in a...g the proportion of energy lost per mean wave cycle...equilibrium state the loss of energy by breaking is comparable to that supplied by the wind, one can estimate the...

1969-01-01T23:59:59.000Z

412

EXPERIMENTAL VALIDATION OF A COUPLED BEM-NAVIER-STOKES MODEL FOR SOLITARY WAVE SHOALING AND BREAKING  

E-Print Network (OSTI)

applications are considered. The first one is the case of the breaking of a solitary wave over a step, which). INTRODUCTION Breaking waves on beaches and over ocean bottom discontinuities, constitute one of the most. In particular, due to the increasing power of mod- ern computers, direct Computational Fluid Dynamics (CFD

Grilli, Stéphan T.

413

Transgenic plants are sensitive bioindicators of nuclear pollution caused by the Chernobyl accident  

SciTech Connect

To evaluate the genetic consequences of radioactive contamination originating from the Nuclear reactor accident of Chernobyl on indigenous populations of plants and animals, it is essential to determine the rates of accumulating genetic changes in chronically irradiated populations. An increase in germline mutation rates in humans living close to the Chernobyl Nuclear Power Plant site, and a two- to tenfold increase in germline mutations in barn swallows breeding in Chernobyl have been reported. Little is known, however, about the effects of chronic irradiation on plant genomes. Ionizing radiation causes double-strand breaks in DNA, which are repaired via illegitimate or homologous recombination. The authors make use of Arabidopsis thaliana plants carrying a {beta}-glucuronidase marker gene as a recombination substrate to monitor genetic alterations in plant populations, which are caused by nuclear pollution of the environment around Chernobyl. A significant increase in somatic intrachromosomal recombination frequencies was observed at nuclear pollution levels from 0.1--900 Ci/km{sup 2}, consistent with an increase in chromosomal aberrations. This bioindicator may serve as a convenient and ethically acceptable alternative to animal systems.

Kovalchuk, I.; Kovalchuk, O. [Ivano-Frankivsk State Medical Academy (Ukraine)]|[Friedrich Miescher Inst., Basel (Switzerland); Arkhipov, A. [Chernobyl Scientific and Technical Center of International Research (Ukraine); Hohn, B. [Friedrich Miescher Inst., Basel (Switzerland)

1998-11-01T23:59:59.000Z

414

An evaluation of genotoxicity in human neuronal-type cells subjected to oxidative stress under an extremely low frequency pulsed magnetic field  

Science Journals Connector (OSTI)

Abstract The possible genotoxicity of extremely low frequency magnetic field (ELF-MF) exposure is still a controversial topic. The most of the reported data suggests that it alone does not affect DNA integrity, but several recent reports have suggested that sinusoidal ELF-MF may increase the effect of known genotoxic agents. Only a few studies deal with non sinusoidal ELF-MF, including pulsed magnetic field (PMF), which are produced by several devices. The aim of this study is to investigate whether PMF exposure can interfere with DNA damage and repair in the presence of a genotoxic oxidative agent in neuronal type cells. To this purpose gamma-H2AX foci formation, which is a sensitive marker of DNA double strand breaks (DSB), was investigated at different points of time (1, 24, 48, 72 h) after the H2O2 treatment (300 ?M for 1 h) under PMF exposure (1 mT, 50 Hz) in human neuroblastoma BE(2)C cells. Moreover, cytotoxicity evaluation, by MTT assay and cell cycle analysis, was performed at various points of time after the treatment. Taken together, results suggest that PMF exposure does not interfere with genotoxicity and cytotoxicity induced by oxidative stress.

Gianfranco Giorgi; Mariangela Lecciso; Miriam Capri; Stella Lukas Yani; Angela Virelli; Ferdinando Bersani; Brunella Del Re

2014-01-01T23:59:59.000Z

415

Slow elimination of phosphorylated histone {gamma}-H2AX from DNA of terminally differentiated mouse heart cells in situ  

SciTech Connect

Phosphorylation of replacement histone H2AX occurs in megabase chromatin domains around double-strand DNA breaks (DSBs) and this modification (called {gamma}-H2AX) may serve as a useful marker of genome damage and repair in terminally differentiated cells. Here using immunohistochemistry we studied kinetics of {gamma}-H2AX formation and elimination in the X-irradiated mouse heart and renal epithelial tissues in situ. Unirradiated tissues have 3-5% {gamma}-H2AX-positive cells and in tissues fixed 1 h after X-irradiation {gamma}-H2AX-positive nuclei are induced in a dose-dependent manner approaching 20-30% after 3 Gy of IR. Analysis of mouse tissues at different times after 3 Gy of IR showed that maximal induction of {gamma}-H2AX in heart is observed 20 min after IR and then is decreased slowly with about half remaining 23 h later. In renal epithelium maximum of the {gamma}-H2AX-positive cells is observed 40 min after IR and then decreases to control values in 23 h. This indicates that there are significant variations between non-proliferating mammalian tissues in the initial H2AX phosphorylation rate as well as in the rate of {gamma}-H2AX elimination after X-irradiation, which should be taken into account in the analysis of radiation responses.

Gavrilov, Boris [Institute of Cytology, Russian Academy of Sciences, 194064 St. Petersburg (Russian Federation); Vezhenkova, Irina [Institute of Cytology, Russian Academy of Sciences, 194064 St. Petersburg (Russian Federation); Firsanov, Denis [Institute of Cytology, Russian Academy of Sciences, 194064 St. Petersburg (Russian Federation); Solovjeva, Liudmila [Institute of Cytology, Russian Academy of Sciences, 194064 St. Petersburg (Russian Federation); Svetlova, Maria [Institute of Cytology, Russian Academy of Sciences, 194064 St. Petersburg (Russian Federation); Mikhailov, Vyacheslav [Institute of Cytology, Russian Academy of Sciences, 194064 St. Petersburg (Russian Federation); Tomilin, Nikolai [Institute of Cytology, Russian Academy of Sciences, 194064 St. Petersburg (Russian Federation)]. E-mail: nvtom@hotmail.com

2006-09-08T23:59:59.000Z

416

Photo of the Week: Record-Breaking Solar Cells | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Photo of the Week: Record-Breaking Solar Cells Photo of the Week: Record-Breaking Solar Cells Photo of the Week: Record-Breaking Solar Cells December 7, 2012 - 2:27pm Addthis Solar Junction, in partnership with NREL, has developed solar cells that reach a record-breaking 44 percent efficiency -- meaning that more than 40 percent of the sunlight the solar cells are exposed to is converted into electrical energy. In this photo, an operator inspects a photolithography tool used to manufacture these solar cells. | Photo by Daniel Derkacs/SolarJunction. Solar Junction, in partnership with NREL, has developed solar cells that reach a record-breaking 44 percent efficiency -- meaning that more than 40 percent of the sunlight the solar cells are exposed to is converted into electrical energy. In this photo, an operator inspects a photolithography

417

Photo of the Week: Record-Breaking Solar Cells | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Record-Breaking Solar Cells Record-Breaking Solar Cells Photo of the Week: Record-Breaking Solar Cells December 7, 2012 - 2:27pm Addthis Solar Junction, in partnership with NREL, has developed solar cells that reach a record-breaking 44 percent efficiency -- meaning that more than 40 percent of the sunlight the solar cells are exposed to is converted into electrical energy. In this photo, an operator inspects a photolithography tool used to manufacture these solar cells. | Photo by Daniel Derkacs/SolarJunction. Solar Junction, in partnership with NREL, has developed solar cells that reach a record-breaking 44 percent efficiency -- meaning that more than 40 percent of the sunlight the solar cells are exposed to is converted into electrical energy. In this photo, an operator inspects a photolithography

418

INTERNAL REPAIR OF GAS PIPLINES SURVEY OF OPERATOR EXPERIENCE AND INDUSTRY NEEDS REPORT  

SciTech Connect

A repair method that can be applied from the inside of a gas transmission pipeline (i.e., a trenchless repair) is an attractive alternative to conventional repair methods since the need to excavate the pipeline is precluded. This is particularly true for pipelines in environmentally sensitive and highly populated areas. The objectives of the project are to evaluate, develop, demonstrate, and validate internal repair methods for pipelines; develop a functional specification for an internal pipeline repair system; and prepare a recommended practice for internal repair of pipelines. The purpose of this survey is to better understand the needs and performance requirements of the natural gas transmission industry regarding internal repair. A total of fifty-six surveys were sent to pipeline operators. A total of twenty completed surveys were returned, representing a 36% response rate, which is considered very good given the fact that tailored surveys are known in the marketing industry to seldom attract more than a 10% response rate. The twenty survey responses produced the following principal conclusions: (1) Use of internal weld repair is most attractive for river crossings, under other bodies of water (e.g., lakes and swamps) in difficult soil conditions, under highways, under congested intersections, and under railway crossings. All these areas tend to be very difficult and very costly if, and where, conventional excavated repairs may be currently used. (2) Internal pipe repair offers a strong potential advantage to the high cost of horizontal direct drilling (HDD) when a new bore must be created to solve a leak or other problem in a water/river crossing. (3) The typical travel distances required can be divided into three distinct groups: up to 305 m (1,000 ft.); between 305 m and 610 m (1,000 ft. and 2,000 ft.); and beyond 914 m (3,000 ft.). In concept, these groups require pig-based systems; despooled umbilical systems could be considered for the first two groups. For the last group a self-propelled system with an onboard self-contained power and welding system is required. (4) Pipe size range requirements range from 50.8 mm (2 in.) through 1,219.2 mm (48 in.) in diameter. The most common size range for 80% to 90% of operators surveyed is 508 mm to 762 mm (20 in. to 30 in.) diameter, with 95% using 558.8 mm (22 in.) diameter pipe.

Ian D. Harris

2003-09-01T23:59:59.000Z

419

Symmetry Breaking, Unification, and Theories Beyond the Standard Model  

SciTech Connect

A model was constructed in which the supersymmetric fine-tuning problem is solved without extending the Higgs sector at the weak scale. We have demonstrated that the model can avoid all the phenomenological constraints, while avoiding excessive fine-tuning. We have also studied implications of the model on dark matter physics and collider physics. I have proposed in an extremely simple construction for models of gauge mediation. We found that the {mu} problem can be simply and elegantly solved in a class of models where the Higgs fields couple directly to the supersymmetry breaking sector. We proposed a new way of addressing the flavor problem of supersymmetric theories. We have proposed a new framework of constructing theories of grand unification. We constructed a simple and elegant model of dark matter which explains excess flux of electrons/positrons. We constructed a model of dark energy in which evolving quintessence-type dark energy is naturally obtained. We studied if we can find evidence of the multiverse.

Nomura, Yasunori

2009-07-31T23:59:59.000Z

420

Treatment methods for breaking certain oil and water emulsions  

DOE Patents (OSTI)

Disclosed are treatment methods for breaking emulsions of petroleum oil and salt water, fatty oil and water, and those resulting from liquefication of organic material. The emulsions are broken by heating to a predetermined temperature at or above about 200.degree. C. and pressurizing to a predetermined pressure above the vapor pressure of water at the predetermined temperature to produce a heated and pressurized fluid. The heated and pressurized fluid is contained in a single vessel at the predetermined temperature and pressure for a predetermined period of time to effectively separate the emulsion into substantially distinct first and second phases, the first phase comprising primarily the petroleum oil, the second phase comprising primarily the water. The first and second phases are separately withdrawn from the vessel at a withdraw temperature between about 200.degree. C. and 374.degree. C. and a withdraw pressure above the vapor pressure of water at the withdraw temperature. Where solids are present in the certain emulsions, the above described treatment may also effectively separate the certain emulsion into a substantially distinct third phase comprising primarily the solids.

Sealock, Jr., L. John (W. Richland, WA); Baker, Eddie G. (Richland, WA); Elliott, Douglas C. (Richland, WA)

1992-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "double-strand break repair" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


421

Spontaneous symmetry breaking in Schroedinger lattices with two nonlinear sites  

SciTech Connect

We introduce discrete systems in the form of straight (infinite) and ring-shaped chains, with two symmetrically placed nonlinear sites. The systems can be implemented in nonlinear optics (as waveguiding arrays) and Bose-Einstein condensates (by means of an optical lattice). A full set of exact analytical solutions for symmetric, asymmetric, and antisymmetric localized modes is found, and their stability is investigated in a numerical form. The symmetry-breaking bifurcation, through which the asymmetric modes emerge from the symmetric ones, is found to be of the subcritical type. It is transformed into a supercritical bifurcation if the nonlinearity is localized in relatively broad domains around two central sites, and also in the ring of a small size, i.e., in effectively nonlocal settings. The family of antisymmetric modes does not undergo bifurcations and features both stable and unstable portions. The evolution of unstable localized modes is investigated by means of direct simulations. In particular, unstable asymmetric states, which exist in the case of the subcritical bifurcation, give rise to breathers oscillating between the nonlinear sites, thus restoring an effective dynamical symmetry between them.

Brazhnyi, Valeriy A.; Malomed, Boris A. [Centro de Fisica do Porto, Faculdade de Ciencias, Universidade do Porto, R. Campo Alegre 687, Porto 4169-007 (Portugal); ICFO-Institut de Ciencies Fotoniques, Universitat Politecnica de Catalunya, Mediterranean Technology Park, 08860 Castelldefels (Barcelona) (Spain) and Department of Physical Electronics, School of Electrical Engineering, Faculty of Engineering, Tel Aviv University, Tel Aviv 69978 (Israel)

2011-05-15T23:59:59.000Z

422

Natural cold baryogenesis from strongly interacting electroweak symmetry breaking  

SciTech Connect

The mechanism of ''cold electroweak baryogenesis'' has been so far unpopular because its proposal has relied on the ad-hoc assumption of a period of hybrid inflation at the electroweak scale with the Higgs acting as the waterfall field. We argue here that cold baryogenesis can be naturally realized without the need to introduce any slow-roll potential. Our point is that composite Higgs models where electroweak symmetry breaking arises via a strongly first-order phase transition provide a well-motivated framework for cold baryogenesis. In this case, reheating proceeds by bubble collisions and we argue that this can induce changes in Chern-Simons number, which in the presence of new sources of CP violation commonly lead to baryogenesis. We illustrate this mechanism using as a source of CP violation an effective dimension-six operator which is free from EDM constraints, another advantage of cold baryogenesis compared to the standard theory of electroweak baryogenesis. Our results are general as they do not rely on any particular UV completion but only on a stage of supercooling ended by a first-order phase transition in the evolution of the universe, which can be natural if there is nearly conformal dynamics at the TeV scale. Besides, baryon-number violation originates from the Standard Model only.

Konstandin, Thomas; Servant, Géraldine, E-mail: tkonstan@cern.ch, E-mail: geraldine.servant@cern.ch [CERN Physics Department, Theory Division, CH-1211 Geneva 23 (Switzerland)

2011-07-01T23:59:59.000Z

423

Physical Naturalness and Dynamical Breaking of Classical Scale Invariance  

E-Print Network (OSTI)

We propose a model of a confining dark sector, dark technicolor, that communicates with the Standard Model through the Higgs portal. In this model electroweak symmetry breaking and dark matter share a common origin, and the electroweak scale is generated dynamically. Our motivation to suggest this model is the absense of evidence for new physics from recent LHC data. Although the conclusion is far from certain at this point, this lack of evidence may suggest that no mechanism exists at the electroweak scale to stabilise the Higgs mass against radiative corrections from UV physics. The usual reaction to this puzzling situation is to conclude that the stabilising new physics is either hidden from us by accident, or that it appears at energies that are currently inaccessible, such that nature is indeed fine-tuned. In order to re-examine the arguments that have lead to this dichotomy, we review the concept of naturalness in effective field theories, discussing in particular the role of quadratic divergences in relation to different energy scales. This leads us to suggest classical scale invariance as a guidline for model building, implying that explicit mass scales are absent in the underlying theory.

Matti Heikinheimo; Antonio Racioppi; Martti Raidal; Christian Spethmann; Kimmo Tuominen

2014-05-05T23:59:59.000Z

424

Automated inspection of surface breaking cracks using GMR sensor arrays  

SciTech Connect

We present a prototype for automated magnetic stray field testing of ferromagnetic roller bearings. For this purpose NDE-adapted GMR sensor arrays (giant magneto resistance) are used for the detection of surface breaking cracks. The sensors are miniaturized down to the lower ?m-regime to achieve adequate spatial resolution. In doing so, sensor arrays with up to 48 elements are used to inspect the bearing surface within a few seconds only. In contrast to magnetic particle inspection (MPI), where the global magnetization requires a further inspection step and succeeding demagnetization, the presented prototype only locally magnetize the surface area in the vicinity of the GMR Sensors. For the local magnetization, the applied sub-surface magnetic field was simulated and proofed for detecting flaws with a depth of a few 10 ?m. By multiplexing the sensor array with an adapted read out electronics we quasi simultaneously detect the normal field component of about 100?m above the surface. The detection of artificial notches with a depth of 40 ?m and more could be resolved with a SNR better than 20 dB. The presented testing facility is fast and provides a step towards automated testing of safety relevant steel components.

Pelkner, Matthias; Reimund, Verena; Erthner, Thomas; Panke, Nicolai; Kreutzbruck, Marc [BAM Federal Institute for Material Research and Testing, Unter den Eichen 87, 12205 Berlin (Germany)

2014-02-18T23:59:59.000Z

425

Long?term variations of ice breaking noise in Antarctica  

Science Journals Connector (OSTI)

Numerous low frequency transient signals arriving from Antarctica have been detected in five?year sea noise recordings in 2002?2006 at the hydroacoustic listening station deployed off Cape Leeuwin in Western Australia as part of the International Monitoring System of the Comprehensive Nuclear?Test?Ban Treaty. The majority of these signals consist of a single arrival of mode 1 which dominates the higher modes and undergoes strong frequency dispersion due to long?range propagation in the polar ocean environment. These signals are believed to be emitted primarily by ice rifting and breaking events on the Antarctic shelf. Several regions along the Antarctic coast associated with certain glaciers and ice tongues were found to persistently be much more active in generatingicenoise than other parts of the Antarctic shelf. An analysis of long?term changes in the frequency of occurrence of ice events showed noticeable seasonal cycles but did not reveal any significant interannual variations against the background of short?term fluctuations. Some of the ice events detected at the Cape Leeuwin station were also identified in sea noise recordings made on autonomous acoustic loggers deployed on the Antarctic shelf which allowed us to investigate characteristics of the acoustic signals from ice events near their origins.

Alexander Gavrilov; Binghui Li

2008-01-01T23:59:59.000Z

426

Scales of Fermion Mass Generation and Electroweak Symmetry Breaking  

E-Print Network (OSTI)

The scale of mass generation for fermions (including neutrinos) and the scale for electroweak symmetry breaking (EWSB) can be bounded from above by the unitarity of scattering involving longitudinal weak gauge bosons or their corresponding would-be Goldstone bosons. Including the exact n-body phase space we analyze the 2 --> n ($n \\geq 2$) processes for the fermion-(anti)fermion scattering into multiple gauge boson final states. Contrary to naive energy power counting, we demonstrate that as $n$ becomes large, the competition between an increasing energy factor and a phase-space suppression leads to a {\\it strong new upper bound} on the scale of fermion mass generation at a finite value $n=n_s$, which is {\\it independent of the EWSB scale,} $v = (\\sqrt{2}G_F)^{-1/2}$. For quarks, leptons and Majorana neutrinos, the strongest 2 --> n limits range from about 3TeV to 130-170TeV (with $2\\lesssim n_s \\lesssim 24$), depending on the measured fermion masses. Strikingly, given the tiny neutrino masses as constrained by the neutrino oscillations, neutrinoless double-beta decays and astrophysical observations, the unitarity violation of $\

Duane A. Dicus; Hong-Jian He

2005-06-11T23:59:59.000Z

427

Intraoperative Sac Pressure Measurement During Endovascular Abdominal Aortic Aneurysm Repair  

SciTech Connect

PurposeIntraoperative sac pressure was measured during endovascular abdominal aortic aneurysm repair (EVAR) to evaluate the clinical significance of sac pressure measurement.MethodsA microcatheter was placed in an aneurysm sac from the contralateral femoral artery, and sac pressure was measured during EVAR procedures in 47 patients. Aortic blood pressure was measured as a control by a catheter from the left brachial artery.ResultsThe systolic sac pressure index (SPI) was 0.87 {+-} 0.10 after main-body deployment, 0.63 {+-} 0.12 after leg deployment (P < 0.01), and 0.56 {+-} 0.12 after completion of the procedure (P < 0.01). Pulse pressure was 55 {+-} 21 mmHg, 23 {+-} 15 mmHg (P < 0.01), and 16 {+-} 12 mmHg (P < 0.01), respectively. SPI showed no significant differences between the Zenith and Excluder stent grafts (0.56 {+-} 0.13 vs. 0.54 {+-} 0.10, NS). Type I endoleak was found in seven patients (15%), and the SPI decreased from 0.62 {+-} 0.10 to 0.55 {+-} 0.10 (P = 0.10) after fixing procedures. Type II endoleak was found in 12 patients (26%) by completion angiography. The SPI showed no difference between type II endoleak positive and negative (0.58 {+-} 0.12 vs. 0.55 {+-} 0.12, NS). There were no significant differences between the final SPI of abdominal aortic aneurysms in which the diameter decreased in the follow-up and that of abdominal aortic aneurysms in which the diameter did not change (0.53 {+-} 0.12 vs. 0.57 {+-} 0.12, NS).ConclusionsSac pressure measurement was useful for instant hemodynamic evaluation of the EVAR procedure, especially in type I endoleaks. However, on the basis of this small study, the SPI cannot be used to reliably predict sac growth or regression.

Ishibashi, Hiroyuki, E-mail: ishibash@aichi-med-u.ac.j [Aichi Medical University Hospital, Department of Vascular Surgery (Japan); Ishiguchi, Tsuneo [Aichi Medical University Hospital, Department of Radiology (Japan); Ohta, Takashi; Sugimoto, Ikuo; Iwata, Hirohide; Yamada, Tetsuya; Tadakoshi, Masao; Hida, Noriyuki; Orimoto, Yuki [Aichi Medical University Hospital, Department of Vascular Surgery (Japan); Kamei, Seiji [Aichi Medical University Hospital, Department of Radiology (Japan)

2010-10-15T23:59:59.000Z

428

Models for Achromatic Light-Curve Breaks in GRB Afterglows: Jets, Structured Outflows, and Energy Injection  

E-Print Network (OSTI)

The steepening (break) of the power-law fall-off observed in the optical emission of some GRB afterglows at epoch ~1 day is often attributed to a collimated outflow (jet), undergoing lateral spreading. Wider opening GRB ejecta with a non-uniform energy angular distribution (structured outflows) or the cessation of energy injection in the afterglow can also yield light-curve breaks. We determine the optical and X-ray light-curve decay indices and spectral energy distribution slopes for 10 GRB afterglows with optical light-curve breaks (980519, 990123, 990510, 991216, 000301, 000926, 010222, 011211, 020813, 030226), and use these properties to test the above models for light-curve steepening. It is found that the optical breaks of six of these afterglows can be accommodated by either energy injection or by structured outflows. In the refreshed shock model, a wind-like stratification of the circumburst medium (as expected for massive stars as GRB progenitors) is slightly favoured. A spreading jet interacting with a homogeneous circumburst medium is required by the afterglows 990510, 000301, 011211, and 030226. The optical pre- and post-break decays of these four afterglows are incompatible with a wind-like medium. The current sample of 10 afterglows with breaks suggests that the distribution of the break magnitude (defined as the increase of the afterglow decay exponent) is bimodal, with a gap at 1. If true, this bimodality favours the structured outflow model, while the gap location indicates a homogeneous circumburst environment.

A. Panaitescu

2005-06-23T23:59:59.000Z

429

Repair of mismatched basepairs in mammalian DNA. Progress report, March 1, 1990--February 28, 1991  

SciTech Connect

We have concentrated on three specific areas of our research plan. Our greatest emphasis is on the role of single strand nicks in influencing template strand selection in mismatch repair. We have found, that the ability of a nick in one strand to influence which strand is repaired is not a simple function of distance from the mismatched site but rather that an hot spot where a nick is more likely to have an influence can exist. The second line was production of single-genotype heteroduplexes in order to examine independently the repair of T/G and A/C mispairs within the same sequence context as in our mixed mispair preparations. We have shown preparations of supercoiled heteroduplex can be prepared that were exclusively T/G or exclusively A/C at the mispair site. The third effort has been to understand the difference in repair bias of different cell lines or different transfection conditions as it may relate to different repair systems in the cell. We have identified some of the sources of variation, including cell cycle position. We hope to continue this work to more precisely identify the phase of the cell cycle.

Taylor, J.H.; Hare, J.T.

1991-08-01T23:59:59.000Z

430

HANFORD SITE LOW EXPOSURE PIPELINE REPAIR USING A NON-METALLIC COMPOSITE SYSTEM  

SciTech Connect

At the Department of Energy, Richland Operations (DOE-RL) Hanford site in eastern Washington, a 350 mm (14 inch) diameter high density polyethylene (HDPE) pump recirculation pipeline failed at a bonded joint adjacent to a radiologically and chemically contaminated groundwater storage basin. The responsible DOE-RL contractor, CH2MHill Plateau Remediation Company, applied a fiberglass reinforced plastic (composite) field repair system to the failed joint. The system was devised specifically for the HDPE pipe repair at the Hanford site, and had not been used on this type of plastic piping previously. This paper introduces the pipe failure scenario, describes the options considered for repair and discusses the ultimate resolution of the problem. The failed pipeline was successfully returned to service with minimal impact on waste water treatment plant operating capacity. Additionally, radiological and chemical exposures to facility personnel were maintained as low as reasonably achievable (ALARA). The repair is considered a success for the near term, and future monitoring will prove whether the repair can be considered for long term service and as a viable alternative for similar piping failures at the Hanford site.

HUTH RJ

2009-11-12T23:59:59.000Z

431

Effectiveness of using fibre-reinforced polymer composites for underwater steel pipeline repairs  

Science Journals Connector (OSTI)

Metal pipelines are the most efficient and safe ways for oil and gas transportation over a long distance. At present, almost all pipelines are made by ferrous steel which is sensitive to corrosion at harsh working environments, particularly in the presence of salty water and sulphur ingress media. For years, the most traditionally-credible solution for a damaged steel pipe is to remove the pipe entirely or just a localised damaged section and then replace it by a new one or cover with a steel patch through welding, respectively. Welding or fixing the steel patch is a bulky process especially if the location is underground or underwater. Thus, many researchers have been striving to find effective and safe repair solutions which are light, fast and easy to handle. Numerous literatures have shown that fibre-reinforced polymer-based composites can be effectively used for steel pipe repairs. Considerable research has been carried out on the repair of corroded and gouged pipes incorporating with fibre-reinforced composite wraps. This paper provides a comprehensive review on the use of fibre-reinforced polymer composites for in-air, underground and underwater pipeline repairs. Future developments and prospects on this are also discussed. Critical aspects of technical challenges, benefits and shortcomings in determining the feasibility and suitability for repair systems involving the composites are also presented.

Md Shamsuddoha; Md Mainul Islam; Thiru Aravinthan; Allan Manalo; Kin-tak Lau

2013-01-01T23:59:59.000Z

432

Breaking Up - And Making Up - Are Hard To Do | U.S. DOE Office of  

NLE Websites -- All DOE Office Websites (Extended Search)

Breaking Up Breaking Up - And Making Up - Are Hard To Do News Featured Articles 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 Science Headlines Presentations & Testimony News Archives Contact Information Office of Science U.S. Department of Energy 1000 Independence Ave., SW Washington, DC 20585 P: (202) 586-5430 12.27.11 Breaking Up - And Making Up - Are Hard To Do But as Office of Science research shows, the results can be electric. Print Text Size: A A A Subscribe FeedbackShare Page It's said that breaking up is hard to do. That's undoubtedly true. And so is making up, as more than a few forlorn hearts have found. But when the lightning does strike, the electricity is lasting. What's true for the affairs of the heart also holds for the affairs of hydrogen, as researchers at the Office of Science's Argonne National

433

TeV scale left-right symmetry with spontaneous D-parity breaking  

SciTech Connect

The different scenarios of spontaneous breaking of D parity have been studied in both the nonsupersymmetric and the supersymmetric version of the left-right symmetric models (LRSM). We explore the possibility of a TeV scale SU(2){sub R} breaking scale M{sub R} and hence TeV scale right-handed neutrinos from both minimization of the scalar potential as well as the coupling constant unification point of view. We show that, although minimization of the scalar potential allows the possibility of a TeV scale M{sub R} and tiny neutrino masses in LRSM with spontaneous D-parity breaking, the gauge coupling unification at a high scale {approx}10{sup 16} GeV does not favor a TeV scale symmetry breaking except in the supersymmetric left-right model with Higgs doublet and bidoublet. The phenomenology of neutrino mass is also discussed.

Borah, Debasish [Indian Institute of Technology Bombay, Mumbai-400076 (India); Patra, Sudhanwa; Sarkar, Utpal [Physical Research Laboratory, Ahmedabad-380009 (India)

2011-02-01T23:59:59.000Z

434

Rapid generation of upwelling at a shelf break caused by buoyancy shutdown  

Science Journals Connector (OSTI)

Model analyses of an along-shelf flow over a continental shelf and slope reveal upwelling near the shelf break. A stratified, initially uniform along-shelf flow undergoes a rapid adjustment with notable differences onshore and offshore of the ...

Jessica Benthuysen; Leif N. Thomas; Steven J. Lentz

435

Electroweak symmetry breaking in the era of the Higgs boson discovery  

E-Print Network (OSTI)

In this thesis we study possible deviations from the electroweak symmetry breaking mechanism as predicted by the Standard Model (SM), and we introduce a new framework to analyze hadronic final states at colliders. In the ...

Bertolini, Daniele, Ph. D. Massachusetts Institute of Technology

2014-01-01T23:59:59.000Z

436

Effect of catechins and tannins on depleted uranium-induced DNA strand breaks  

Science Journals Connector (OSTI)

The effects of polyphenols on plasmid DNA strand breaks by depleted uranium were studied using four catechins: (+)...2 2+) with hydrogen peroxide (H2O2) were strongly enhanced by EGC, EGCG, MMT, a...

Emiko Matsuda; Akira Nakajima

2012-08-01T23:59:59.000Z

437

Break-up of Pt catalyst surfaces by high CO coverage  

E-Print Network (OSTI)

and atomic arrangement of catalyst surface under reactionof reactant molecules on the catalyst surfaces are the norm.Break-up of Pt catalyst surfaces by high CO coverage Feng

Tao, Feng

2010-01-01T23:59:59.000Z

438

Symmetry breaking in O+4: an application of the Brueckner coupled-cluster method  

Science Journals Connector (OSTI)

A recent calculation of the antisymmetric stretch frequency for the rectangular structure of quartet O+4 using the QCISD(T) method gave a value of 3710 cm?1. This anomalous frequency is shown to be a consequence of symmetry-breaking effects, which occur even though the QCISD(T) solution derived from a delocalized SCF reference function lies energetically well below the two localized (symmetry-broken) solutions at the equilibrium geometry. The symmetry breaking is almost eliminated at the CCSD level of theory, but the small remaining symmetry-breaking effects are magnified at the CCSD(T) level of theory so that the antisymmetric stretch frequency is still significantly in error. The Brueckner coupled-cluster method, however, leads to a symmetrical solution which is free of symmetry-breaking effects, with an antisymmetric stretch frequency of 1322 cm?1, in good agreement with our earlier calculations using the CASSCF/CASSI method.

Leslie A. Barnes; Roland Lindh

1994-01-01T23:59:59.000Z

439

Physics and learning based computational models for breaking bow waves based on new boundary immersion approaches  

E-Print Network (OSTI)

A ship moving on the free surface produces energetic breaking bow waves which generate spray and air entrainment. Present experimental, analytic, and numerical studies of this problem are costly, inaccurate and not robust. ...

Weymouth, Gabriel David

2008-01-01T23:59:59.000Z

440

Numerical evidence of turbulence generated by non-breaking surface waves  

Science Journals Connector (OSTI)

Numerical simulation of monochromatic surface waves propagating over a turbulent field is conducted to reveal the mechanism of turbulence production by non-breaking waves. The numerical model solves the primitive equations subject to the fully ...

Wu-ting Tsai; Shi-ming Chen; Guan-hung Lu

Note: This page contains sample records for the topic "double-strand break repair" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


441

Introduction of Break-Out Session at the International PV Module Quality Assurance Forum (Presentation)  

SciTech Connect

This presentation outlines review requirements for quality assurance (QA) rating systems, logical design of QA systems, and specific tasks for break-out session 1 of the 2011 International PV Module Quality Assurance Forum.

Kurtz, S.; Wohlgemuth, J.; Yamamichi, M.; Sample, T.

2011-07-01T23:59:59.000Z

442

Double Ended Guillotine Break in a Prismatic Block VHTR Lower Plenum Air Ingress Scenario  

E-Print Network (OSTI)

The double ended guillotine break leading to density-driven air ingress has been identified as a low probability yet high consequence event for Very High Temperature Reactor (VHTR). The lower plenum of the VHTR contains the core support structure...

Hartley, Jessica

2012-10-19T23:59:59.000Z

443

Fluid-bubble interaction and dissipation mechanisms under unsteady breaking waves  

E-Print Network (OSTI)

Fluid-bubble interaction and dissipation mechanisms under unsteady breaking waves of air in bubbles that are believed to suppress liquid phase turbulence to incorporate entrained bubble populations using an Eulerian-Eulerian formulation

Kirby, James T.

444

U.S. gasoline prices decreases for 16th week in a row; breaking...  

U.S. Energy Information Administration (EIA) Indexed Site

18, 2015 U.S. gasoline prices decreases for 16th week in a row; breaking previous record set in 2008 (long version) The U.S. average retail price for regular gasoline fell 7.3...

445

U.S. gasoline prices decreases for 16th week in a row; breaking...  

U.S. Energy Information Administration (EIA) Indexed Site

gasoline prices decreases for 16th week in a row; breaking previous record set in 2008 (short version) The U.S. average retail price for regular gasoline fell 7.3 cents from a week...

446

E-Print Network 3.0 - anomaly-mediated supersymmetry breaking...  

NLE Websites -- All DOE Office Websites (Extended Search)

; Physics 58 arXiv:hep-ph0103095v19Mar2001 Low-Energy Supersymmetry and its Phenomenology Summary: . This approach has been called anomaly-mediated SUSY-breaking (AMSB)....

447

Substitutional Routing for Trunk Line Break-Down in the Teletransmission Network  

Science Journals Connector (OSTI)

Trunk-line break-down in teletransmission network interrupts some number of the routes of some different links. It makes necessary to restore, at least partially, failed transmission possibilities for each affect...

dr ing. Kornel Wydro

1988-01-01T23:59:59.000Z

448

Spring Break Course in Florida! Forensic entomology is a branch of forensic sci-  

E-Print Network (OSTI)

Spring Break Course in Florida! Forensic entomology is a branch of forensic sci- ence utilizing the science of forensic en- tomology through daily laboratory and field ac- tivities. We also interact

449

Microsoft PowerPoint - Bull Shoals U1 repair MSB edit 3.ppt  

NLE Websites -- All DOE Office Websites (Extended Search)

Bull Shoals Unit 1 161kV Bull Shoals Unit 1 161kV Switchyard Feeder Repair Bull Shoals Unit 1 161kV Bull Shoals Unit 1 161kV Switchyard Feeder Repair Switchyard Feeder Repair Mark Dixson Little Rock District US Army Corps US Army Corps of Engineers of Engineers ® ® One Corps Serving The Army and the Nation * Original Equipment - 52 years old. * Unit 1 Oil filled cable pothead failed and exploded on Sept 6 2006. * Resulting fire and fire suppression damaged oil insulated cable system * Minor damage to power plant. Background Background Background US Army Corps US Army Corps of Engineers of Engineers ® ® One Corps Serving The Army and the Nation * Transformer Unit 2 damaged and removed from service. * Cause of fire - Failure of Pothead stress cone assembly and build up of combustible gas * Power Plant lost all power and went in the

450

Emailed Comments of Elizabeth Chimento RE: Pepco Scheduled Line Repair Dec.  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Emailed Comments of Elizabeth Chimento RE: Pepco Scheduled Line Emailed Comments of Elizabeth Chimento RE: Pepco Scheduled Line Repair Dec. 1-20, 2006 Emailed Comments of Elizabeth Chimento RE: Pepco Scheduled Line Repair Dec. 1-20, 2006 Docket No. EO-05-01. Regarding the planned line outage, scheduled for Feb. 20 through Mar. 6, I am requesting that the residents near the plant be moved for the duration of the outage to protect their health. Emailed Comments of Elizabeth Chimento RE: Pepco Scheduled Line Repair Dec. 1-20, 2006 More Documents & Publications Letter from Elizabeth Chimento and Poul Hertel to the Federal Energy Regulatory Commission Re: District of Columbia Public Service Commission Emergency Petition and Complaint Docket No. EL05-145-000 Re: Potomac River Generating Station Department of Energy Case No.

451

Structural and Functional Studies on Nucleotide Excision Repair From Recognition to Incision.  

SciTech Connect

Maintenance of the correct genetic information is crucial for all living organisms because mutations are the primary cause of hereditary diseases, as well as cancer and may also be involved in aging. The importance of genomic integrity is underscored by the fact that 80 to 90% of all human cancers are ultimately due to DNA damage. Among the different repair mechanisms that have evolved to protect the genome, nucleotide excision repair (NER) is a universal pathway found in all organisms. NER removes a wide variety of bulky DNA adducts including the carcinogenic cyclobutane pyrimidine dimers induced by UV radiation, benzo(a)pyrene-guanine adducts caused by smoking and the guanine-cisplatin adducts induced by chemotherapy. The importance of this repair mechanism is reflected by three severe inherited diseases in humans, which are due to defects in NER: xeroderma pigmentosum, Cockayne's syndrome and trichothiodystrophy.

Caroline Kisker

2001-01-01T23:59:59.000Z

452

Evaluation of bisphenol E cyanate ester for the resin-injection repair of advanced composites  

SciTech Connect

This thesis is a compilation of a general introduction and literature review that ties together the subsequent chapters which consist of two journal articles that have yet to be submitted for publication. The overall topic relates to the evaluation and application of a new class of cyanate ester resin with unique properties that lend it applicable to use as a resin for injection repair of high glass transition temperature polymer matrix composites. The first article (Chapter 2) details the evaluation and optimization of adhesive properties of this cyanate ester and alumina nanocomposites under different conditions. The second article (Chapter 3) describes the development and evaluation of an injection repair system for repairing delaminations in polymer matrix composites.

Wilber Yaote Lio

2009-12-19T23:59:59.000Z

453

A survey of repair practices for nuclear power plant containment metallic pressure boundaries  

SciTech Connect

The Nuclear Regulatory Commission has initiated a program at the Oak Ridge National Laboratory to provide assistance in their assessment of the effects of potential degradation on the structural integrity and leaktightness of metal containment vessels and steel liners of concrete containments in nuclear power plants. One of the program objectives is to identify repair practices for restoring metallic containment pressure boundary components that have been damaged or degraded in service. This report presents issues associated with inservice condition assessments and continued service evaluations and identifies the rules and requirements for the repair and replacement of nonconforming containment pressure boundary components by welding or metal removal. Discussion topics include base and welding materials, welding procedure and performance qualifications, inspection techniques, testing methods, acceptance criteria, and documentation requirements necessary for making acceptable repairs and replacements so that the plant can be returned to a safe operating condition.

Oland, C.B.; Naus, D.J. [Oak Ridge National Lab., TN (United States)

1998-05-01T23:59:59.000Z

454

Live Cell Imaging and in situ analysis of cellular responses to DNA doublestrand  

NLE Websites -- All DOE Office Websites (Extended Search)

Live Cell Imaging and in situ analysis of cellular responses to DNA double- Live Cell Imaging and in situ analysis of cellular responses to DNA double- strand breaks in mammalian cells. David J. Chen Division of Molecular Radiation Biology, Department of Radiation Oncology, University of Texas Southwestern Medical Center, Dallas TX 75390 The integrity of the human genome is constantly threatened by internal as well as external factors with the propensity to cause DNA damage. Of the various types of DNA damage that can occur within the mammalian cell nucleus, the DNA double strand break (DSB) is perhaps the most dangerous. Estimates put the number of endogenous DSBs anywhere between 10-100 per nucleus per day. A direct link between DSBs and cancer has been surmised by researchers based upon the fact that many cancer-predisposition

455

Global methylation responses to low dose radiation exposure  

NLE Websites -- All DOE Office Websites (Extended Search)

methylation responses to low dose radiation exposure methylation responses to low dose radiation exposure Pamela J Sykes, Michelle R Newman, Benjamin J Blyth and Rebecca J Ormsby Haematology and Genetic Pathology, Flinders University and Medical Centre, Flinders Centre for Cancer Prevention and Control, Bedford Park, Adelaide, South Australia 5042 Australia. (pam.sykes@flinders.edu.au). Our goal is to study the mechanisms involved in biological responses to low doses of radiation in vivo in the dose range that is relevant to population and occupational exposures. At high radiation doses, DNA double-strand breaks are considered the critical lesion underlying the initiation of radiation-induced carcinogenesis. However, at the very low radiation doses relevant for the general public, the induction of DNA double-strand breaks

456

Simulation of a small break loss of coolant accident conducted at the BETHSY Integral Test Facility  

E-Print Network (OSTI)

. The computer code RELAP5/MOD3 was used to model the BETHSY Integral Test Facility for a. small break loss of coolant accident. This transient simulates a 2 inch cold leg break without high pressure safety injection, following the conditions of International..., and general input to my gra, duate education. TABLE OF CONTENTS CHAPTER Page I INTRODUCTION I. 1 Need for Investigation I. 2 Computational Modeling . I. 3 Experimental Modeling I, 4 International Cooperation . 1 3 RELAP5 CODE DESCRIPTION II. 1...

Bott, Charles Patrick

1992-01-01T23:59:59.000Z

457

Functions of disordered regions in mammalian early base excision repair proteins  

Science Journals Connector (OSTI)

Reactive oxygen species, generated endogenously and induced as a toxic response, produce several dozen oxidized or modified bases and/or single-strand breaks in mammalian and other genomes. These lesions are pred...

Muralidhar L. Hegde; Tapas K. Hazra; Sankar Mitra

2010-11-01T23:59:59.000Z

458

Hurricane-damaged Gulf of Mexico pipeline repaired with cold forging  

SciTech Connect

Damage to Texaco Pipeline Inc.'s Eugene Island Pipeline System (EIPS) in last year's Hurricane Andrew prompted a complex repair project unique for the Gulf of Mexico. Damage, suffered when the anchor of a runaway semisubmersible drilling rig crashed into the 20-in. EPIS during the height of the storm, caused the pipeline to fail under pressure within 48 hr. after start-up following the storm. The paper describes the importance of the EIPS; system safety; Andrew's damage; locating the leak; repair options; the chosen system; mechanical bonding; end connectors and ball flanges; and diving operations.

Lewis, G. (Texaco Pipeline Inc., Houma, LA (United States)); DeGruy, P. (Texaco Inc., New Orleans, LA (United States)); Avery, L. (Big Inch Marine Systems Inc., Lafayette, LA (United States))

1993-05-03T23:59:59.000Z

459

EVALUATION OF A UAV COMPOSITE WING SPAR REPAIR USING AN EMBEDDED OPTICAL FIBER RAYLEIGH BACK-SCATTERING DISTRIBUTED STRAIN SENSING  

E-Print Network (OSTI)

EVALUATION OF A UAV COMPOSITE WING SPAR REPAIR USING AN EMBEDDED OPTICAL FIBER RAYLEIGH BACK Branch, Tel-Aviv Israel ikressel@iai.co.il ABSTRACT A representing damaged UAV wing spar cap was repaired requirements for UAVs, where the substantiation of structural bonded joint can be based on: "repeatable

Paris-Sud XI, Université de

460

Nearest-neighbor distributions of free radicals produced within charged-particle tracks in liquid water  

E-Print Network (OSTI)

for 20-MeV alpha particles compared to maximum double strand break distance INTRODUCTION All forms of ionizing radiation eventually produce charged particles which are responsible for depositing all of the energy to the medium being irradiated. We... quantify this energy deposition in terms of absorbed dose. The definition of absorbed dose is the energy absorbed per unit mass from any kind of ionizing radiation in any type of matter (2). It has long been recognized that different types of radiation...

Smith, Miles Clay

2012-06-07T23:59:59.000Z

Note: This page contains sample records for the topic "double-strand break repair" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


461

SMITH, GLEN. Bond Characteristics and Qualifications of Adhesives for Marine Applications and Steel Pipe Repair. (Under the direction of Dr. Sami Rizkalla.)  

E-Print Network (OSTI)

for marine application and repair of steel pipeline. #12;BOND CHARACTERISTICS AND QUALIFICATIONS OF ADHESIVES and Steel Pipe Repair. (Under the direction of Dr. Sami Rizkalla.) Performance of adhesives significantly FOR MARINE APPLICATIONS AND STEEL PIPE REPAIR By Glen E. Smith A thesis submitted to the Graduate Faculty

462

Multilayer sulfur-resistant composite metal membranes and methods of making and repairing the same  

DOE Patents (OSTI)

The invention relates to thin, hydrogen-permeable, sulfur-resistant membranes formed from multi-layers of palladium or palladium-alloy coatings on porous, ceramic or metal supports, methods of making these membranes, methods of repairing layers of these membranes and devices that incorporate these membranes.

Way, J. Douglas; Hatlevik, Oyvind

2014-07-15T23:59:59.000Z

463

Investigation of Corrosion Damage and Repair System in a Concrete Jetty Structure  

E-Print Network (OSTI)

Investigation of Corrosion Damage and Repair System in a Concrete Jetty Structure Farid Moradi of the deterioration was reinforcement corrosion, which initiated within few years after the completion of the jetty structure. The structure underwent major corrosion rehabilitation after 7 years of service. Despite the use

Mobasher, Barzin

464

Evaluation of Hardware and Procedures for Astronaut Assembly and Repair of Large Precision Reflectors  

Science Journals Connector (OSTI)

A detailed procedure is presented that enables astronauts in extravehicular activity (EVA) to efficiently assemble and repair large (i.e., greater than 10m-diameter) segmented reflectors, supported by a truss, for space-based optical or radio-frequency ...

Lake Mark S.; Jr Walter L. Heard; Watson Judith J.; Collins Timothy J.

2000-08-01T23:59:59.000Z

465

Latest advances in steam turbine design, blading, repairs, condition assessment, and condenser interaction  

SciTech Connect

This book contains papers presented at a conference on power generation. Topics covered include: a life extension approach for steam turbine blading in Electricite de France fossil plants, and on site 430 MW high pressure reheat turbine shell cracking and distortion repairs.

Rasmussen, D.M. (Turbine Consultants, Inc., Milwaukee, WI (US))

1989-01-01T23:59:59.000Z

466

Evolutionary neural network modeling for forecasting the field failure data of repairable systems  

Science Journals Connector (OSTI)

An accurate product reliability prediction model can not only learn and track the product's reliability and operational performance, but also offer useful information for managers to take follow-up actions to improve the product' quality and cost. This ... Keywords: Genetic algorithms, Neural network model, Reliability prediction, Repairable system

L. Yi-Hui

2007-11-01T23:59:59.000Z

467

Rational Design of Human DNA Ligase Inhibitors that Target Cellular DNA Replication and Repair  

Science Journals Connector (OSTI)

...agents to treat human cancer. Grant support: U.S. NIH grants GM47251, ES12512, and GM57479...Biology of DNA Repair Program grant CA92584 (A.E. Tomkinson...University of Maryland, School of Pharmacy Computer-Aided Drug Design Center...

Xi Chen; Shijun Zhong; Xiao Zhu; Barbara Dziegielewska; Tom Ellenberger; Gerald M. Wilson; Alexander D. MacKerell, Jr.; and Alan E. Tomkinson

2008-05-01T23:59:59.000Z

468

Recombination between a defective retrovirus and homologous sequences in host DNA: reversion by patch repair.  

Science Journals Connector (OSTI)

...transcriptase assay (16). Virus infections were car- ried out with the addition of polybrene...NIH/3T3 cells were infected with the defective virions as described above, but these...infected cell. Thus, we have shown that defective viruses can repair their mutations by...

P Schwartzberg; J Colicelli; S P Goff

1985-03-01T23:59:59.000Z

469

Evaluation of temporary non-code repairs in safety class 3 piping systems  

SciTech Connect

Temporary non-ASME Code repairs in safety class 3 pipe and piping components are permissible during plant operation in accordance with Nuclear Regulatory Commission Generic Letter 90-05. However, regulatory acceptance of such repairs requires the licensee to undertake several timely actions. Consistent with the requirements of GL 90-05, this paper presents an overview of the detailed evaluation and relief request process. The technical criteria encompasses both ductile and brittle piping materials. It also lists appropriate evaluation methods that a utility engineer can select to perform a structural integrity assessment for design basis loading conditions to support the use of temporary non-Code repair for degraded piping components. Most use of temporary non-code repairs at a nuclear generating station is in the service water system which is an essential safety related system providing the ultimate heat sink for various plant systems. Depending on the plant siting, the service water system may use fresh water or salt water as the cooling medium. Various degradation mechanisms including general corrosion, erosion/corrosion, pitting, microbiological corrosion, galvanic corrosion, under-deposit corrosion or a combination thereof continually challenge the pressure boundary structural integrity. A good source for description of corrosion degradation in cooling water systems is provided in a cited reference.

Godha, P.C.; Kupinski, M.; Azevedo, N.F. [Northeast Utilities System, Hartford, CT (United States)

1996-12-01T23:59:59.000Z

470

Overview of NCHRP Design Guideline for EPS-Block Geofoam in Slope Stabilization and Repair  

E-Print Network (OSTI)

1 Overview of NCHRP Design Guideline for EPS-Block Geofoam in Slope Stabilization and Repair David in Construction Applications (EPS 2011 Norway) June 6-8, 2011 #12;2 ABSTRACT This paper presents an overview of the design guideline for the use of expanded polystyrene (EPS)-block geofoam for slope stabilization

Horvath, John S.

471

Development/Plasticity/Repair A Structural MRI Study of Human Brain Development from  

E-Print Network (OSTI)

Development/Plasticity/Repair A Structural MRI Study of Human Brain Development from Birth to 2 Carolina, Chapel Hill, North Carolina 27599-7510 Brain development in the first 2 years after birth and schizophrenia. Knowledge regarding this period is currently quite limited. We studied structural brain

Utah, University of

472

Self-repairing Self-assembled Structures Daniel Arbuckle and Aristides A. G. Requicha  

E-Print Network (OSTI)

1 Self-repairing Self-assembled Structures Daniel Arbuckle and Aristides A. G. Requicha Department.arbuckle@usc.edu, requicha@usc.edu Abstract-- This paper introduces a method by which structures can be self-assembled from by a process analogous to mitosis. I. INTRODUCTION Self-assembly agents have the potential to become an im

Southern California, University of

473

Home Maintenance and Repair Owning a home is a major responsibility.  

E-Print Network (OSTI)

Home Maintenance and Repair Owning a home is a major responsibility. Not only do you have the obligation of paying the mortgage each month, but you also have to maintain your home so that it remains a good investment. When talking about taking care of your home, most folks lump home maintenance

474

Analysis and Repair of Name Tagger Errors Heng Ji Ralph Grishman  

E-Print Network (OSTI)

Analysis and Repair of Name Tagger Errors Heng Ji Ralph Grishman Department of Computer Science New feedback from subsequent stages in an information extraction pipeline: name structure parsing, cross' pipelined system archi- tectures, NE tagging is one of the first steps in the pipeline. NE errors adversely

475

CAN-bus based distributed control system for hydraulic turbine blade repairing robot  

Science Journals Connector (OSTI)

A CAN-bus (Controller Area Network) based distributed control system was designed for a mobile on-site hydraulic turbine blade repairing robot (HTBRR). The system is composed of one supervisory station, seven joint servo nodes, and four accessory nodes. ... Keywords: distributed control system, mobile robot, virtual reality

Kangtai Xiang; Zhenguo Sun; Hongjun Dai; Qiang Chen; Jiajun Liu

2010-11-01T23:59:59.000Z

476

Repair of DNA Alkylation Induced in L1210 Leukemia and Murine Bone Marrow by Three Chloroethylnitrosoureas  

Science Journals Connector (OSTI)

...cross-links proceeds much more slowly than does repair of monoadducts; we observed that...cross-links. Further, removal of label does not ensure that the full process of excision...Sartorelli and D. G. Johns (eds.). Handbook of Experimental Pharma cology, Vol. 38...

James D. Ahlgren; Dianna C. Green; Kenneth D. Tew; and Philip S. Schein

1982-07-01T23:59:59.000Z

477

Repairing and maintaining a public bicycle system: the case of Vlib' in Paris1  

E-Print Network (OSTI)

Repairing and maintaining a public bicycle system: the case of Vélib' in Paris1 . Martin Tironi Phd. Valued for their 'planet friendly' character, public bicycle systems have become the hallmark for cities to the worldwide spread of public bicycle schemes (PBS). They now exist in more than 110 cities around the world

Boyer, Edmond

478

Persistence of gamma-H2AX and 53BP1 foci in proliferating and nonproliferating human mammary epithelial cells after exposure to gamma-rays or iron ions  

SciTech Connect

To investigate {gamma}-H2AX (phosphorylated histone H2AX) and 53BP1 (tumour protein 53 binding protein No. 1) foci formation and removal in proliferating and non-proliferating human mammary epithelial cells (HMEC) after exposure to sparsely and densely ionizing radiation under different cell culture conditions. HMEC cells were grown either as monolayers (2D) or in extracellular matrix to allow the formation of acinar structures in vitro (3D). Foci numbers were quantified by image analysis at various time points after exposure. Our results reveal that in non-proliferating cells under 2D and 3D cell culture conditions, iron-ion induced {gamma}-H2AX foci were still present at 72 h after exposure, although 53BP1 foci returned to control levels at 48 h. In contrast in proliferating HMEC, both {gamma}-H2AX and 53BP1 foci decreased to control levels during the 24-48 h time interval after irradiation under 2D conditions. Foci numbers decreased faster after {gamma}-ray irradiation and returned to control levels by 12 h regardless of marker, cell proliferation status, and cell culture condition. Conclusions: The disappearance of radiation induced {gamma}-H2AX and 53BP1 foci in HMEC have different dynamics that depend on radiation quality and proliferation status. Notably, the general patterns do not depend on the cell culture condition (2D versus 3D). We speculate that the persistent {gamma}-H2AX foci in iron-ion irradiated non-proliferating cells could be due to limited availability of double strand break (DSB) repair pathways in G0/G1-phase, or that repair of complex DSB requires replication or chromatin remodeling.

Groesser, Torsten; Chang, Hang; Fontenay, Gerald; Chen, James; Costes, Sylvain V.; Barcellos-Hoff, Mary Helen; Parvin, Bahram; Rydberg, Bjorn

2010-12-22T23:59:59.000Z

479

C-terminal region of DNA ligase IV drives XRCC4/DNA ligase IV complex to chromatin  

SciTech Connect

Highlights: •Chromatin binding of XRCC4 is dependent on the presence of DNA ligase IV. •C-terminal region of DNA ligase IV alone can recruit itself and XRCC4 to chromatin. •Two BRCT domains of DNA ligase IV are essential for the chromatin binding of XRCC4. -- Abstract: DNA ligase IV (LIG4) and XRCC4 form a complex to ligate two DNA ends at the final step of DNA double-strand break (DSB) repair through non-homologous end-joining (NHEJ). It is not fully understood how these proteins are recruited to DSBs. We recently demonstrated radiation-induced chromatin binding of XRCC4 by biochemical fractionation using detergent Nonidet P-40. In the present study, we examined the role of LIG4 in the recruitment of XRCC4/LIG4 complex to chromatin. The chromatin binding of XRCC4 was dependent on the presence of LIG4. The mutations in two BRCT domains (W725R and W893R, respectively) of LIG4 reduced the chromatin binding of LIG4 and XRCC4. The C-terminal fragment of LIG4 (LIG4-CT) without N-terminal catalytic domains could bind to chromatin with XRCC4. LIG4-CT with W725R or W893R mutation could bind to chromatin but could not support the chromatin binding of XRCC4. The ability of C-terminal region of LIG4 to interact with chromatin might provide us with an insight into the mechanisms of DSB repair through NHEJ.

Liu, Sicheng; Liu, Xunyue; Kamdar, Radhika Pankaj; Wanotayan, Rujira; Sharma, Mukesh Kumar [Research Laboratory for Nuclear Reactors and Department of Nuclear Engineering, Graduate School of Science and Engineering, Tokyo Institute of Technology, Tokyo 152-8550 (Japan)] [Research Laboratory for Nuclear Reactors and Department of Nuclear Engineering, Graduate School of Science and Engineering, Tokyo Institute of Technology, Tokyo 152-8550 (Japan); Adachi, Noritaka [Graduate School of Nanobioscience, Yokohama City University, Yokohama 236-0027 (Japan)] [Graduate School of Nanobioscience, Yokohama City University, Yokohama 236-0027 (Japan); Matsumoto, Yoshihisa, E-mail: yoshim@nr.titech.ac.jp [Research Laboratory for Nuclear Reactors and Department of Nuclear Engineering, Graduate School of Science and Engineering, Tokyo Institute of Technology, Tokyo 152-8550 (Japan)] [Research Laboratory for Nuclear Reactors and Department of Nuclear Engineering, Graduate School of Science and Engineering, Tokyo Institute of Technology, Tokyo 152-8550 (Japan)

2013-09-20T23:59:59.000Z

480

Repair-dependent cell radiation survival and transformation: an integrated theory  

Science Journals Connector (OSTI)

The repair-dependent model of cell radiation survival is extended to include radiation-induced transformations. The probability of transformation is presumed to scale with the number of potentially lethal damages that are repaired in a surviving cell or the interactions of such damages. The theory predicts that at doses corresponding to high survival, the transformation frequency is the sum of simple polynomial functions of dose; linear, quadratic, etc, essentially as described in widely used linear-quadratic expressions. At high doses, corresponding to low survival, the ratio of transformed to surviving cells asymptotically approaches an upper limit. The low dose fundamental- and high dose plateau domains are separated by a downwardly concave transition region. Published transformation data for mammalian cells show the high-dose plateaus predicted by the repair-dependent model for both ultraviolet and ionizing radiation. For the neoplastic transformation experiments that were analyzed, the data can be fit with only the repair-dependent quadratic function. At low doses, the transformation frequency is strictly quadratic, but becomes sigmodial over a wider range of doses. Inclusion of data from the transition region in a traditional linear-quadratic analysis of neoplastic transformation frequency data can exaggerate the magnitude of, or create the appearance of, a linear component. Quantitative analysis of survival and transformation data shows good agreement for ultraviolet radiation; the shapes of the transformation components can be predicted from survival data. For ionizing radiations, both neutrons and x-rays, survival data overestimate the transforming ability for low to moderate doses. The presumed cause of this difference is that, unlike UV photons, a single x-ray or neutron may generate more than one lethal damage in a cell, so the distribution of such damages in the population is not accurately described by Poisson statistics. However, the complete sigmodial dose-response data for neoplastic transformations can be fit using the repair-dependent functions with all parameters determined only from transformation frequency data.

John C Sutherland

2014-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "double-strand break repair" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


481

Effects of $?$-cluster breaking on 3$?$ cluster structures in $^{12}$C  

E-Print Network (OSTI)

To clarify the effects of $\\alpha$-cluster breaking on 3$\\alpha$ cluster structures in $^{12}$C, we investigate $^{12}$C using a hybrid model that combines the Brink-Bloch cluster model with the $p_{3/2}$ subshell closure wave function. We have found that $\\alpha$-cluster breaking caused by spin-orbit force significantly changes cluster structures of excited $0^{+}$ states through orthogonality to lower states. Spatially developed cluster components of the $0^{+}_{2}$ state are reduced. The $0^{+}_{3}$ state changes from a vibration mode in the bending motion of three $\\alpha$ clusters to a chain-like 3$\\alpha$ structure having an open triangle configuration. As a result of these structure changes of $0^{+}$ states, the band assignment for the $2^{+}_{2}$ state is changed by the $\\alpha$-cluster breaking effect. Namely, in model calculations without the $\\alpha$-cluster breaking effect, the $0^{+}_{2}$ state is assigned to be the band-head of the $2^{+}_{2}$ state. However, when we incorporate $\\alpha$-cluster breaking caused by the spin-orbit force, the $0^{+}_{3}$ state is regarded as the band-head of the $2^{+}_{2}$ state.

Tadahiro Suhara; Yoshiko Kanada-En'yo

2014-11-05T23:59:59.000Z

482

The noise spectral parameters and the energy of breaking waves experimental study  

Science Journals Connector (OSTI)

The process of wave breaking and whitecap creation is one of the most important and least understood phenomena associated with the evolution of the surface gravity waves in the open sea. This process is the main way of energy and momentum transfer between ocean and atmosphere. However it is very difficult to estimate under real sea conditions the frequency of breaking wave events or the fraction of sea surface covered by whitecaps and the amount of dissipated energy produced by wave breaking. A controlled experiment was carried out in the Ocean Basin Laboratory at MARINTEK Trondheim (Norway). The simulation of random waves of the prescribed spectra provided a very realistic pattern of the sea surface. The number of breaking waves was estimated using photography method and wave staff recording. Acoustic measurements during the experiments were conducted in order to examine the relationship between the noise spectral parameters and both the whitecap coverage and dissipation energy of breaking waves for different types of waves. A comparison of simultaneous video observations wave staff records of the surface wave above the hydrophones with the spectral parameters of acoustical signals made it possible to find physical links between processes.

2003-01-01T23:59:59.000Z

483

Breaking Down Enzymes' Role in Biofuel Production | U.S. DOE Office of  

Office of Science (SC) Website

Breaking Down Enzymes' Role in Biofuel Production Breaking Down Enzymes' Role in Biofuel Production Biological and Environmental Research (BER) BER Home About Research Facilities Science Highlights Searchable Archive of BER Highlights External link Benefits of BER Funding Opportunities Biological & Environmental Research Advisory Committee (BERAC) News & Resources Contact Information Biological and Environmental Research U.S. Department of Energy SC-23/Germantown Building 1000 Independence Ave., SW Washington, DC 20585 P: (301) 903-3251 F: (301) 903-5051 E: sc.ber@science.doe.gov More Information » February 2013 Breaking Down Enzymes' Role in Biofuel Production Molecular structures provide insights into biomass deconstruction. Print Text Size: A A A Subscribe FeedbackShare Page Click to enlarge photo. Enlarge Photo

484

Record-breaking year for patents | Y-12 National Security Complex  

NLE Websites -- All DOE Office Websites (Extended Search)

Record-breaking year for ... Record-breaking year for ... Record-breaking year for patents Posted: February 11, 2013 - 2:57pm | Y-12 Report | Volume 9, Issue 2 | 2013 Fiscal 2012 was a fruitful year for innovative research and development at the Y-12 National Security Complex. Site inventors were issued an unprecedented 11 patents. Technologies and processes from many of Y-12's latest patents, like those from previous years, can extend beyond the Nuclear Security Enterprise into diverse areas of private industry. One technology, the measurement system Extrans, determines how much water permeates a material. At Y-12 this measuring method can protect moisture-sensitive material from corroding. In the food or pharmaceutical industry, however, Extrans could test product packaging to ensure that no

485

Breaking a Cycle-Free Lifestyle for Earth Day | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Breaking a Cycle-Free Lifestyle for Earth Day Breaking a Cycle-Free Lifestyle for Earth Day Breaking a Cycle-Free Lifestyle for Earth Day April 12, 2012 - 12:07pm Addthis Elizabeth Spencer Communicator, National Renewable Energy Laboratory Earth Day is just around the corner! And while it might not be a major day for most people, I still think it's kind of fun. I think every special day is an excuse to do something you normally wouldn't! (And, speaking of little somethings, the Energy Efficiency and Renewable Energy website is going to have a small page up to commemorate Earth Day this year. Look for that next week.) This Earth Day, I'm buying a new bicycle! Now, before I start, let me get this out of the way: Bikes and I have not historically gotten along. When I was a young girl, I was so thrilled to

486

Breaking a Cycle-Free Lifestyle for Earth Day | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Breaking a Cycle-Free Lifestyle for Earth Day Breaking a Cycle-Free Lifestyle for Earth Day Breaking a Cycle-Free Lifestyle for Earth Day April 12, 2012 - 12:07pm Addthis Elizabeth Spencer Communicator, National Renewable Energy Laboratory Earth Day is just around the corner! And while it might not be a major day for most people, I still think it's kind of fun. I think every special day is an excuse to do something you normally wouldn't! (And, speaking of little somethings, the Energy Efficiency and Renewable Energy website is going to have a small page up to commemorate Earth Day this year. Look for that next week.) This Earth Day, I'm buying a new bicycle! Now, before I start, let me get this out of the way: Bikes and I have not historically gotten along. When I was a young girl, I was so thrilled to

487

DuPont Technology Breaks Away From Glass | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

DuPont Technology Breaks Away From Glass DuPont Technology Breaks Away From Glass DuPont Technology Breaks Away From Glass April 22, 2010 - 4:20pm Addthis Delaware-based DuPont is working to develop ultra-thin moisture protective films for photovoltaic panels - so thin they're about 1,000 times thinner than a human hair. DuPont is working on new photovoltaic technology that will let manufacturers of copper indium gallium selenide, or CIGS, solar cells and organic light emitting diodes, or OLED, displays protect products with thin layers of ceramic and polymer material instead of glass. These ultra-thin protective films could help prevent deterioration from moisture. Because of their potential to reduce the cost of producing solar energy, "thin-film PV modules are projected to be the fastest-growing segment of

488

Largest Federally-Owned Wind Farm Breaks Ground at U.S. Weapons Facility |  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Largest Federally-Owned Wind Farm Breaks Ground at U.S. Weapons Largest Federally-Owned Wind Farm Breaks Ground at U.S. Weapons Facility Largest Federally-Owned Wind Farm Breaks Ground at U.S. Weapons Facility August 13, 2013 - 10:54am Addthis News Media Contact (202) 586-4940 WASHINGTON - Building on President Obama's Climate Action Plan, which calls for steady, responsible steps to reduce carbon pollution, the Energy Department today broke ground on the nation's largest federally-owned wind project at the Pantex Plant in Amarillo, Texas. Once completed, this five-turbine 11.5 megawatt project will power more than 60 percent of the plant with clean, renewable wind energy and reduce carbon emissions by over 35,000 metric tons per year - equivalent to taking 7,200 cars off the road. The Pantex Plant is the primary site for the assembly, disassembly,

489

Largest Federally-Owned Wind Farm Breaks Ground at U.S. Weapons Facility |  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Largest Federally-Owned Wind Farm Breaks Ground at U.S. Weapons Largest Federally-Owned Wind Farm Breaks Ground at U.S. Weapons Facility Largest Federally-Owned Wind Farm Breaks Ground at U.S. Weapons Facility August 13, 2013 - 10:54am Addthis News Media Contact (202) 586-4940 WASHINGTON - Building on President Obama's Climate Action Plan, which calls for steady, responsible steps to reduce carbon pollution, the Energy Department today broke ground on the nation's largest federally-owned wind project at the Pantex Plant in Amarillo, Texas. Once completed, this five-turbine 11.5 megawatt project will power more than 60 percent of the plant with clean, renewable wind energy and reduce carbon emissions by over 35,000 metric tons per year - equivalent to taking 7,200 cars off the road. The Pantex Plant is the primary site for the assembly, disassembly,

490

Breaking Up (Hydrogen) No Longer As Hard To Do | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Breaking Up (Hydrogen) No Longer As Hard To Do Breaking Up (Hydrogen) No Longer As Hard To Do Breaking Up (Hydrogen) No Longer As Hard To Do December 29, 2011 - 1:12pm Addthis Researchers at Argonne National Lab have recently developed a process to improve the efficiency of producing hydrogen to run cars such as this prototype, which was developed at the Oakridge National Lab. | Photo courtesy of Oak Ridge National Laboratory. Researchers at Argonne National Lab have recently developed a process to improve the efficiency of producing hydrogen to run cars such as this prototype, which was developed at the Oakridge National Lab. | Photo courtesy of Oak Ridge National Laboratory. Charles Rousseaux Charles Rousseaux Senior Writer, Office of Science What does this project do? Researchers have new insight into producing pure hydrogen -- a

491

DuPont Technology Breaks Away From Glass | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

DuPont Technology Breaks Away From Glass DuPont Technology Breaks Away From Glass DuPont Technology Breaks Away From Glass April 22, 2010 - 4:20pm Addthis Delaware-based DuPont is working to develop ultra-thin moisture protective films for photovoltaic panels - so thin they're about 1,000 times thinner than a human hair. DuPont is working on new photovoltaic technology that will let manufacturers of copper indium gallium selenide, or CIGS, solar cells and organic light emitting diodes, or OLED, displays protect products with thin layers of ceramic and polymer material instead of glass. These ultra-thin protective films could help prevent deterioration from moisture. Because of their potential to reduce the cost of producing solar energy, "thin-film PV modules are projected to be the fastest-growing segment of

492

Breaking Up (Hydrogen) No Longer As Hard To Do | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Breaking Up (Hydrogen) No Longer As Hard To Do Breaking Up (Hydrogen) No Longer As Hard To Do Breaking Up (Hydrogen) No Longer As Hard To Do December 29, 2011 - 1:12pm Addthis Researchers at Argonne National Lab have recently developed a process to improve the efficiency of producing hydrogen to run cars such as this prototype, which was developed at the Oakridge National Lab. | Photo courtesy of Oak Ridge National Laboratory. Researchers at Argonne National Lab have recently developed a process to improve the efficiency of producing hydrogen to run cars such as this prototype, which was developed at the Oakridge National Lab. | Photo courtesy of Oak Ridge National Laboratory. Charles Rousseaux Charles Rousseaux Senior Writer, Office of Science What does this project do? Researchers have new insight into producing pure hydrogen -- a

493

The concepts of leak before break and absolute reliability of NPP equipment and piping  

SciTech Connect

This paper describes the absolute reliability (AR) concept for ensuring safe operation of nuclear plant equipment and piping. The AR of a pipeline or component is defined as the level of reliability when the probability of an instantaneous double-ended break is near zero. AR analysis has been applied to Russian RBMK and VVER type reactors. It is proposed that analyses required for application of the leak before break concept should be included in AR implementation. The basic principles, methods, and approaches that provide the basis for implementing the AR concept are described.

Getman, A.F.; Komarov, O.V.; Sokov, L.M. [and others

1997-04-01T23:59:59.000Z

494

Small break LOCA analysis of the ONRL high flux isotope reactor  

SciTech Connect

A digital simulation program, HFIRSYS, was developed using MMS to analyze small break loss of coolant events in the ORNL High Flux Isotope Reactor. The code evaluates the response of the primary reactor system including automatic controls actions resulting from breaks in auxiliary piping connected to the primary. The primary output of the code is the margin to the onset of nucleate boiling expressed as a ratio of heat flux which would cause boiling to the current hot channel heat flux. A description of the model, validation results and a sample transient are presented.

Wilson, T.L. Jr.; Cook, D.H.; Sozer, A.

1988-01-01T23:59:59.000Z

495

Isospin Symmetry Breaking and Octet Baryon Masses due to Their Mixing with Decuplet Baryons  

E-Print Network (OSTI)

We study the isospin symmetry breaking and mass splittings of the eight lowest-lying baryons. We consider three kinds of baryon mass terms, including the bare mass term, the electromagnetic terms and the spontaneous chiral symmetry breaking terms. We include the mixing term between flavor-octet and flavor-decuplet baryons. This assumes that the lowest-lying Sigma and Xi baryons contain a few decuplet components and so are not purely flavor-octet. We achieve a good fitting that the difference between every fitted mass and its experimental value is less than 0.2 MeV.

Hua-Xing Chen

2013-12-05T23:59:59.000Z