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Sample records for double strand breaks

  1. Zinc chromate induces chromosome instability and DNA double strand breaks in human lung cells

    SciTech Connect (OSTI)

    Xie Hong; Holmes, Amie L.; Young, Jamie L.; Qin Qin; Joyce, Kellie; Pelsue, Stephen C.; Peng Cheng; Wise, Sandra S.; Jeevarajan, Antony S.; Wallace, William T.; Hammond, Dianne; Wise, John Pierce E-mail: John.Wise@usm.maine.edu

    2009-02-01

    Hexavalent chromium Cr(VI) is a respiratory toxicant and carcinogen, with solubility playing an important role in its carcinogenic potential. Zinc chromate, a water insoluble or 'particulate' Cr(VI) compound, has been shown to be carcinogenic in epidemiology studies and to induce tumors in experimental animals, but its genotoxicity is poorly understood. Our study shows that zinc chromate induced concentration-dependent increases in cytotoxicity, chromosome damage and DNA double strand breaks in human lung cells. In response to zinc chromate-induced breaks, MRE11 expression was increased and ATM and ATR were phosphorylated, indicating that the DNA double strand break repair system was initiated in the cells. In addition, our data show that zinc chromate-induced double strand breaks were only observed in the G2/M phase population, with no significant amount of double strand breaks observed in G1 and S phase cells. These data will aid in understanding the mechanisms of zinc chromate toxicity and carcinogenesis.

  2. Probability of double-strand breaks in genome-sized DNA by {gamma}-ray decreases markedly as the DNA concentration increases

    SciTech Connect (OSTI)

    Shimobayashi, Shunsuke F.; Iwaki, Takafumi; Mori, Toshiaki; Yoshikawa, Kenichi

    2013-05-07

    By use of the single-molecule observation, we count the number of DNA double-strand breaks caused by {gamma}-ray irradiation with genome-sized DNA molecules (166 kbp). We find that P{sub 1}, the number of double-strand breaks (DSBs) per base pair per unit Gy, is nearly inversely proportional to the DNA concentration above a certain threshold DNA concentration. The inverse relationship implies that the total number of DSBs remains essentially constant. We give a theoretical interpretation of our experimental results in terms of attack of reactive species upon DNA molecules, indicating the significance of the characteristics of genome-sized giant DNA as semiflexible polymers for the efficiency of DSBs.

  3. Double stranded nucleic acid biochips

    DOE Patents [OSTI]

    Chernov, Boris; Golova, Julia

    2006-05-23

    This invention describes a new method of constructing double-stranded DNA (dsDNA) microarrays based on the use of pre-synthesized or natural DNA duplexes without a stem-loop structure. The complementary oligonucleotide chains are bonded together by a novel connector that includes a linker for immobilization on a matrix. A non-enzymatic method for synthesizing double-stranded nucleic acids with this novel connector enables the construction of inexpensive and robust dsDNA/dsRNA microarrays. DNA-DNA and DNA-protein interactions are investigated using the microarrays.

  4. Induction and Rejoining of DNA Double Strand Breaks Assessed by H2AX Phosphorylation in Melanoma Cells Irradiated with Proton and Lithium Beams

    SciTech Connect (OSTI)

    Ibanez, Irene L.; Bracalente, Candelaria; Molinari, Beatriz L.; Palmieri, Monica A.; Policastro, Lucia; Kreiner, Andres J.; Burlon, Alejandro A.; Valda, Alejandro; Navalesi, Daniela; Davidson, Jorge; Davidson, Miguel; Vazquez, Monica; Ozafran, Mabel; Duran, Hebe

    2009-07-15

    Purpose: The aim of this study was to evaluate the induction and rejoining of DNA double strand breaks (DSBs) in melanoma cells exposed to low and high linear energy transfer (LET) radiation. Methods and Materials: DSBs and survival were determined as a function of dose in melanoma cells (B16-F0) irradiated with monoenergetic proton and lithium beams and with a gamma source. Survival curves were obtained by clonogenic assay and fitted to the linear-quadratic model. DSBs were evaluated by the detection of phosphorylated histone H2AX ({gamma}H2AX) foci at 30 min and 6 h post-irradiation. Results: Survival curves showed the increasing effectiveness of radiation as a function of LET. {gamma}H2AX labeling showed an increase in the number of foci vs. dose for all the radiations evaluated. A decrease in the number of foci was found at 6 h post-irradiation for low LET radiation, revealing the repair capacity of DSBs. An increase in the size of {gamma}H2AX foci in cells irradiated with lithium beams was found, as compared with gamma and proton irradiations, which could be attributed to the clusters of DSBs induced by high LET radiation. Foci size increased at 6 h post-irradiation for lithium and proton irradiations in relation with persistent DSBs, showing a correlation with surviving fraction. Conclusions: Our results showed the response of B16-F0 cells to charged particle beams evaluated by the detection of {gamma}H2AX foci. We conclude that {gamma}H2AX foci size is an accurate parameter to correlate the rejoining of DSBs induced by different LET radiations and radiosensitivity.

  5. Defects in the kinetics of the repair of DNA double-strand breaks and inhibition of DNA synthesis in the ataxia telangiectasia AT5Bl-VA cell line: Comparison to a corrected hybrid, atxbc

    SciTech Connect (OSTI)

    Kysela, B.P.; Lohrer, H.; Arrand, J.E.

    1995-12-01

    The nature of the primary biochemical defect in the human radiosensitive and cancer-prone syndrome, ataxia telangiectasia (AT), has remained obscure despite many efforts to elucidate it. In this study, AT complementation group D cells and a nearly isogenic corrected AT-hamster hybrid derivative have been analyzed for induction and repair of initial double-strand breaks (DSBs) after exposure to ionizing radiation, using a sensitive field-inversion electrophoresis technique. Results suggesting that initial levels of damage are the same in these two cell types, but indicating differences in the fast component of DNA repair, have been compared and correlated with those resulting from a study of the radioresistant DNA synthesis defect and its correction in the same cell lines. These measurements show that the radioresistant phenotype of the substantially corrected AT-hamster hybrid correlates with its higher level of fast-component DSB repair and higher level of inhibition of DNA synthesis, but that the initial damage induction does not contribute to the phenotype. We propose that the AT gene product(s) is likely to act early in a signaling pathway which controls both DNA repair and progression of cells through the phases of the cell cycle in response to ionizing radiation. 36 refs., 3 figs., 2 tabs.

  6. Solid phase sequencing of double-stranded nucleic acids

    DOE Patents [OSTI]

    Fu, Dong-Jing; Cantor, Charles R.; Koster, Hubert; Smith, Cassandra L.

    2002-01-01

    This invention relates to methods for detecting and sequencing of target double-stranded nucleic acid sequences, to nucleic acid probes and arrays of probes useful in these methods, and to kits and systems which contain these probes. Useful methods involve hybridizing the nucleic acids or nucleic acids which represent complementary or homologous sequences of the target to an array of nucleic acid probes. These probe comprise a single-stranded portion, an optional double-stranded portion and a variable sequence within the single-stranded portion. The molecular weights of the hybridized nucleic acids of the set can be determined by mass spectroscopy, and the sequence of the target determined from the molecular weights of the fragments. Nucleic acids whose sequences can be determined include nucleic acids in biological samples such as patient biopsies and environmental samples. Probes may be fixed to a solid support such as a hybridization chip to facilitate automated determination of molecular weights and identification of the target sequence.

  7. Method of preparing and applying single stranded DNA probes to double stranded target DNAs in situ

    DOE Patents [OSTI]

    Gray, Joe W.; Pinkel, Daniel

    1991-01-01

    A method is provided for producing single stranded non-self-complementary nucleic acid probes, and for treating target DNA for use therewith. Probe is constructed by treating DNA with a restriction enzyme and an exonuclease to form template/primers for a DNA polymerase. The digested strand is resynthesized in the presence of labeled nucleoside triphosphate precursor. Labeled single stranded fragments are separated from the resynthesized fragments to form the probe. Target DNA is treated with the same restriction enzyme used to construct the probe, and is treated with an exonuclease before application of the probe. The method significantly increases the efficiency and specificity of hybridization mixtures by increasing effective probe concentration by eliminating self-hybridization between both probe and target DNAs, and by reducing the amount of target DNA available for mismatched hybridizations.

  8. Method of preparing and applying single stranded DNA probes to double stranded target DNAs in situ

    DOE Patents [OSTI]

    Gray, J.W.; Pinkel, D.

    1991-07-02

    A method is provided for producing single stranded non-self-complementary nucleic acid probes, and for treating target DNA for use therewith. The probe is constructed by treating DNA with a restriction enzyme and an exonuclease to form template/primers for a DNA polymerase. The digested strand is resynthesized in the presence of labeled nucleoside triphosphate precursor. Labeled single stranded fragments are separated from the resynthesized fragments to form the probe. Target DNA is treated with the same restriction enzyme used to construct the probe, and is treated with an exonuclease before application of the probe. The method significantly increases the efficiency and specificity of hybridization mixtures by increasing effective probe concentration by eliminating self-hybridization between both probe and target DNAs, and by reducing the amount of target DNA available for mismatched hybridizations. No Drawings

  9. Spin transport and spin polarization properties in double-stranded DNA

    SciTech Connect (OSTI)

    Simchi, Hamidreza; Esmaeilzadeh, Mahdi Mazidabadi, Hossein

    2013-11-21

    We study the spin-dependent electron transport through a double-stranded DNA (dsDNA) using the Bogoliubov-de Gennes equations and non-equilibrium Green's function method. We calculate the spin-dependent electron conductance and spin-polarization for different lengths, helix angles, twist angles of dsDNA, the environment-induced dephasing factors, and hopping integral. It is shown that the conductance decreases by increasing the length and dephasing factor. Also, we show that the spin-polarization depends on the helical symmetry and the length of DNA. It is shown that the double-stranded DNA can act as a perfect spin filter. Finally, we show that the sign of spin polarization can be inverted from +1 (−1) to −1 (+1) for some values of hopping integral.

  10. Screening Tool for Providers of Double-Stranded DNA - Energy Innovation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Portal Startup America Startup America Industrial Technologies Industrial Technologies Energy Analysis Energy Analysis Biomass and Biofuels Biomass and Biofuels Find More Like This Return to Search Screening Tool for Providers of Double-Stranded DNA Oak Ridge National Laboratory Contact ORNL About This Technology Publications: PDF Document Publication 11-G00227_ID2510 (2).pdf (829 KB) Technology Marketing SummaryThe invention screens purchase orders submitted to DNA synthesis

  11. The effect of a magnetic field on the spin-selective transport in double-stranded DNA

    SciTech Connect (OSTI)

    Simchi, Hamidreza; Esmaeilzadeh, Mahdi Mazidabadi, Hossein

    2014-05-28

    Spin-polarization in double-stranded DNA is studied in the presence of a magnetic field applied along its helix axis using the non-equilibrium Green's function method. The spin-polarization could be tuned by changing the magnetic field. In some special cases, the double-stranded DNA behaved as a perfect spin-filter. Furthermore, the dependency of the spin-polarization on the spin-orbit strength and dephasing strength is studied.

  12. Binding of undamaged double stranded DNA to vaccinia virus uracil-DNA glycosylase

    SciTech Connect (OSTI)

    Schormann, Norbert; Banerjee, Surajit; Ricciardi, Robert; Chattopadhyay, Debasish

    2015-06-02

    Background: Uracil-DNA glycosylases are evolutionarily conserved DNA repair enzymes. However, vaccinia virus uracil-DNA glycosylase (known as D4), also serves as an intrinsic and essential component of the processive DNA polymerase complex during DNA replication. In this complex D4 binds to a unique poxvirus specific protein A20 which tethers it to the DNA polymerase. At the replication fork the DNA scanning and repair function of D4 is coupled with DNA replication. So far, DNA-binding to D4 has not been structurally characterized. Results: This manuscript describes the first structure of a DNA-complex of a uracil-DNA glycosylase from the poxvirus family. This also represents the first structure of a uracil DNA glycosylase in complex with an undamaged DNA. In the asymmetric unit two D4 subunits bind simultaneously to complementary strands of the DNA double helix. Each D4 subunit interacts mainly with the central region of one strand. DNA binds to the opposite side of the A20-binding surface on D4. In comparison of the present structure with the structure of uracil-containing DNA-bound human uracil-DNA glycosylase suggests that for DNA binding and uracil removal D4 employs a unique set of residues and motifs that are highly conserved within the poxvirus family but different in other organisms. Conclusion: The first structure of D4 bound to a truly non-specific undamaged double-stranded DNA suggests that initial binding of DNA may involve multiple non-specific interactions between the protein and the phosphate backbone.

  13. Binding of undamaged double stranded DNA to vaccinia virus uracil-DNA glycosylase

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Schormann, Norbert; Banerjee, Surajit; Ricciardi, Robert; Chattopadhyay, Debasish

    2015-06-02

    Background: Uracil-DNA glycosylases are evolutionarily conserved DNA repair enzymes. However, vaccinia virus uracil-DNA glycosylase (known as D4), also serves as an intrinsic and essential component of the processive DNA polymerase complex during DNA replication. In this complex D4 binds to a unique poxvirus specific protein A20 which tethers it to the DNA polymerase. At the replication fork the DNA scanning and repair function of D4 is coupled with DNA replication. So far, DNA-binding to D4 has not been structurally characterized. Results: This manuscript describes the first structure of a DNA-complex of a uracil-DNA glycosylase from the poxvirus family. This alsomore » represents the first structure of a uracil DNA glycosylase in complex with an undamaged DNA. In the asymmetric unit two D4 subunits bind simultaneously to complementary strands of the DNA double helix. Each D4 subunit interacts mainly with the central region of one strand. DNA binds to the opposite side of the A20-binding surface on D4. In comparison of the present structure with the structure of uracil-containing DNA-bound human uracil-DNA glycosylase suggests that for DNA binding and uracil removal D4 employs a unique set of residues and motifs that are highly conserved within the poxvirus family but different in other organisms. Conclusion: The first structure of D4 bound to a truly non-specific undamaged double-stranded DNA suggests that initial binding of DNA may involve multiple non-specific interactions between the protein and the phosphate backbone.« less

  14. Proximity-induced superconductivity effect in a double-stranded DNA

    SciTech Connect (OSTI)

    Simchi, Hamidreza; Esmaeilzadeh, Mahdi Mazidabadi, Hossein

    2014-02-07

    We study the proximity-induced superconductivity effect in a double-stranded DNA by solving the Bogoliubov-de Gennes equations and taking into account the effect of thermal fluctuations of the twist angle between neighboring base pairs. We show that the electron conductance is spin-dependent and the conductance of spin up (down) increases (decreases) due to the spin-orbit coupling (SOC). It is found that, for T < 100 K, the band gap energy is temperature-independent and it decreases due to the SOC. In addition, by solving the Bogoliubov-de Gennes equations and local gap parameter equation self-consistently, we find the critical temperature at which transition to superconductivity can take place.

  15. Double-stranded DNA organization in bacteriophage heads: An alternative toroid-based model

    SciTech Connect (OSTI)

    Hud, N.V.

    1995-10-01

    Studies of the organization of double-stranded DNA within bacteriophage heads during the past four decades have produced a wealth of data. However, despite the presentation of numerous models, the true organization of DNA within phage heads remains unresolved. The observations of toroidal DNA structures in electron micrographs of phage lysates have long been cited as support for the organization of DNA in a spool-like fashion. This particular model, like all other models, has not been found to be consistent with all available data. Recently, the authors proposed that DNA within toroidal condensates produced in vitro is organized in a manner significantly different from that suggested by the spool model. This new toroid model has allowed the development of an alternative model for DNA organization within bacteriophage heads that is consistent with a wide range of biophysical data. Here the authors propose that bacteriophage DNA is packaged in a toroid that is folded into a highly compact structure.

  16. Double-stranded RNA transcribed from vector-based oligodeoxynucleotide acts as transcription factor decoy

    SciTech Connect (OSTI)

    Xiao, Xiao; Gang, Yi; Wang, Honghong; Wang, Jiayin; Zhao, Lina; Xu, Li; Liu, Zhiguo

    2015-02-06

    Highlights: • A shRNA vector based transcription factor decoy, VB-ODN, was designed. • VB-ODN for NF-κB inhibited cell viability in HEK293 cells. • VB-ODN inhibited expression of downstream genes of target transcription factors. • VB-ODN may enhance nuclear entry ratio for its feasibility of virus production. - Abstract: In this study, we designed a short hairpin RNA vector-based oligodeoxynucleotide (VB-ODN) carrying transcription factor (TF) consensus sequence which could function as a decoy to block TF activity. Specifically, VB-ODN for Nuclear factor-κB (NF-κB) could inhibit cell viability and decrease downstream gene expression in HEK293 cells without affecting expression of NF-κB itself. The specific binding between VB-ODN produced double-stranded RNA and NF-κB was evidenced by electrophoretic mobility shift assay. Moreover, similar VB-ODNs designed for three other TFs also inhibit their downstream gene expression but not that of themselves. Our study provides a new design of decoy for blocking TF activity.

  17. Chemical repair of base lesions, AP-sites, and strand breaks on plasmid DNA in dilute aqueous solution by ascorbic acid

    SciTech Connect (OSTI)

    Hata, Kuniki; Advanced Science Research Center, Japan Atomic Energy Agency, 2-4 Shirakatashirane, Tokai-mura, Naka-gun, Ibaraki 319-1195 ; Urushibara, Ayumi; Yamashita, Shinichi; Shikazono, Naoya; Yokoya, Akinari; Katsumura, Yosuke; Nuclear Professional School, School of Engineering, The University of Tokyo, 2-22 Shirakatashirane, Tokai-mura, Naka-gun, Ibaraki 319-1188

    2013-05-03

    Highlights: We report a novel mechanism of radiation protection of DNA by chemical activity of ascorbic acid. The chemical repair of DNA damage was revealed using biochemical assay and chemical kinetics analysis. We found that ascorbic acid significantly repairs precursors of nucleobase lesions and abasic sites. However, ascorbic acid seldom repairs precursors of DNA-strand breaks. -- Abstract: We quantified the damage yields produced in plasmid DNA by ?-irradiation in the presence of low concentrations (10100 ?M) of ascorbic acid, which is a major antioxidant in living systems, to clarify whether it chemically repairs radiation damage in DNA. The yield of DNA single strand breaks induced by irradiation was analyzed with agarose gel electrophoresis as conformational changes in closed circular plasmids. Base lesions and abasic sites were also observed as additional conformational changes by treating irradiated samples with glycosylase proteins. By comparing the suppression efficiencies to the induction of each DNA lesion, in addition to scavenging of the OH radicals derived from water radiolysis, it was found that ascorbic acid promotes the chemical repair of precursors of AP-sites and base lesions more effectively than those of single strand breaks. We estimated the efficiency of the chemical repair of each lesion using a kinetic model. Approximately 5060% of base lesions and AP-sites were repaired by 10 ?M ascorbic acid, although strand breaks were largely unrepaired by ascorbic acid at low concentrations. The methods in this study will provide a route to understanding the mechanistic aspects of antioxidant activity in living systems.

  18. Activation of double-stranded RNA-dependent protein kinase inhibits proliferation of pancreatic ?-cells

    SciTech Connect (OSTI)

    Chen, Shan-Shan [Key Laboratory of Human Functional Genomics of Jiangsu Province, Nanjing Medical University, Nanjing (China) [Key Laboratory of Human Functional Genomics of Jiangsu Province, Nanjing Medical University, Nanjing (China); Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing (China); Jiang, Teng [Department of Neurology, Qingdao Municipal Hospital, Nanjing Medical University, Nanjing (China)] [Department of Neurology, Qingdao Municipal Hospital, Nanjing Medical University, Nanjing (China); Wang, Yi; Gu, Li-Ze [Key Laboratory of Human Functional Genomics of Jiangsu Province, Nanjing Medical University, Nanjing (China) [Key Laboratory of Human Functional Genomics of Jiangsu Province, Nanjing Medical University, Nanjing (China); Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing (China); Wu, Hui-Wen [Laboratory Center for Basic Medical Sciences, Nanjing Medical University, Nanjing (China)] [Laboratory Center for Basic Medical Sciences, Nanjing Medical University, Nanjing (China); Tan, Lan [Department of Neurology, Qingdao Municipal Hospital, Nanjing Medical University, Nanjing (China)] [Department of Neurology, Qingdao Municipal Hospital, Nanjing Medical University, Nanjing (China); Guo, Jun, E-mail: Guoj@njmu.edu.cn [Key Laboratory of Human Functional Genomics of Jiangsu Province, Nanjing Medical University, Nanjing (China) [Key Laboratory of Human Functional Genomics of Jiangsu Province, Nanjing Medical University, Nanjing (China); Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing (China)

    2014-01-17

    Highlights: PKR can be activated by glucolipitoxicity and pro-inflammatory cytokines in ?-cells. Activated PKR inhibited ?-cell proliferation by arresting cell cycle at G1 phase. Activated PKR fully abrogated the pro-proliferative effects of IGF-I on ?-cells. -- Abstract: Double-stranded RNA-dependent protein kinase (PKR) is revealed to participate in the development of insulin resistance in peripheral tissues in type 2 diabetes (T2DM). Meanwhile, PKR is also characterized as a critical regulator of cell proliferation. To date, no study has focused on the impact of PKR on the proliferation of pancreatic ?-cells. Here, we adopted insulinoma cell lines and mice islet ?-cells to investigate: (1) the effects of glucolipotoxicity and pro-inflammatory cytokines on PKR activation; (2) the effects of PKR on proliferation of pancreatic ?-cells and its underlying mechanisms; (3) the actions of PKR on pro-proliferative effects of IGF-I and its underlying pathway. Our results provided the first evidence that PKR can be activated by glucolipitoxicity and pro-inflammatory cytokines in pancreatic ?-cells, and activated PKR significantly inhibited cell proliferation by arresting cell cycle at G1 phase. Reductions in cyclin D1 and D2 as well as increases in p27 and p53 were associated with the anti-proliferative effects of PKR, and proteasome-dependent degradation took part in the reduction of cyclin D1 and D2. Besides, PKR activation abrogated the pro-proliferative effects of IGF-I by activating JNK and disrupting IRS1/PI3K/Akt signaling pathway. These findings indicate that the anti-proliferative actions of PKR on pancreatic ?-cells may contribute to the pathogenesis of T2DM.

  19. Spatio-temporal evolution and breaking of double layers: A description using Lagrangian hydrodynamics

    SciTech Connect (OSTI)

    Kaw, Predhiman; Sengupta, Sudip; Singh Verma, Prabal [Institute for Plasma Research, Bhat, Gandhinagar 382 428 (India)

    2012-10-15

    The nonlinear development and collapse (breaking) of double layers in the long scale length limit is well described by equations for the cold ion fluid with quasineutrality. It is shown that electron dynamics is responsible for giving an 'equation of state' with negative ratio of specific heats to this fluid. Introducing a transformation for the density variable, the governing equation for the transformed quantity in terms of Lagrange variables turns out exactly to be a linear partial differential equation. This equation has been analyzed in various limits of interest. Nonlinear development of double layers with a sinusoidal initial disturbance and collapse of double layers with an initial perturbation in the form of a density void are analytically investigated.

  20. Mutations Abrogating VP35 Interaction with Double-Stranded RNA Render Ebola Virus Avirulent in Guinea Pigs

    SciTech Connect (OSTI)

    Prins, Kathleen C.; Delpeut, Sebastien; Leung, Daisy W.; Reynard, Olivier; Volchkova, Valentina A.; Reid, St. Patrick; Ramanan, Parameshwaran; Cárdenas, Washington B.; Amarasinghe, Gaya K.; Volchkov, Viktor E.; Basler, Christopher F.

    2010-10-11

    Ebola virus (EBOV) protein VP35 is a double-stranded RNA (dsRNA) binding inhibitor of host interferon (IFN)-{alpha}/{beta} responses that also functions as a viral polymerase cofactor. Recent structural studies identified key features, including a central basic patch, required for VP35 dsRNA binding activity. To address the functional significance of these VP35 structural features for EBOV replication and pathogenesis, two point mutations, K319A/R322A, that abrogate VP35 dsRNA binding activity and severely impair its suppression of IFN-{alpha}/{beta} production were identified. Solution nuclear magnetic resonance (NMR) spectroscopy and X-ray crystallography reveal minimal structural perturbations in the K319A/R322A VP35 double mutant and suggest that loss of basic charge leads to altered function. Recombinant EBOVs encoding the mutant VP35 exhibit, relative to wild-type VP35 viruses, minimal growth attenuation in IFN-defective Vero cells but severe impairment in IFN-competent cells. In guinea pigs, the VP35 mutant virus revealed a complete loss of virulence. Strikingly, the VP35 mutant virus effectively immunized animals against subsequent wild-type EBOV challenge. These in vivo studies, using recombinant EBOV viruses, combined with the accompanying biochemical and structural analyses directly correlate VP35 dsRNA binding and IFN inhibition functions with viral pathogenesis. Moreover, these studies provide a framework for the development of antivirals targeting this critical EBOV virulence factor.

  1. Homologous recombination contributes to the repair of DNA double-strand breaks induced by high-energy iron ions

    SciTech Connect (OSTI)

    Zafar, Faria; Seidler, Sara B.; Kronenberg, Amy; Schild, David; Wiese, Claudia

    2010-06-29

    To test the contribution of homologous recombinational repair (HRR) in repairing DNA damaged sites induced by high-energy iron ions, we used: (1) HRR-deficient rodent cells carrying a deletion in the RAD51D gene and (2) syngeneic human cells impaired for HRR by RAD51D or RAD51 knockdown using RNA interference. We show that in response to iron ions, HRR contributes to cell survival in rodent cells, and that HRR-deficiency abrogates RAD51 foci formation. Complementation of the HRR defect by human RAD51D rescues both enhanced cytotoxicity and RAD51 foci formation. For human cells irradiated with iron ions, cell survival is decreased, and, in p53 mutant cells, the levels of mutagenesis are increased when HRR is impaired. Human cells synchronized in S phase exhibit more pronounced resistance to iron ions as compared with cells in G1 phase, and this increase in radioresistance is diminished by RAD51 knockdown. These results implicate a role for RAD51-mediated DNA repair (i.e. HRR) in removing a fraction of clustered lesions induced by charged particle irradiation. Our results are the first to directly show the requirement for an intact HRR pathway in human cells in ensuring DNA repair and cell survival in response to high-energy high LET radiation.

  2. Analysis of a double-ended cold-leg break simulation: THTF Test 3. 05. 5B. [PWR

    SciTech Connect (OSTI)

    Craddick, W.G.; Pevey, R.E.

    1982-09-01

    On July 3, 1980, an experiment was performed in the Oak Ridge National Laboratory Thermal-Hydraulic Test Facility that simulated a double-ended cold-leg break pressurized-water reactor (PWR) accident. Analysis of the experiment revealed that nuclear fuel rods exposed to the same hydrodynamic environment as that which existed in the experiment would have departed from nucleate boiling both earlier and later than the fuel rod simulator (FRS), depending on the size of the gap between the nuclear fuel pellets and cladding and on the initial power of the nuclear fuel rod. Comparison of the results of the current experiment, which used an FRS bundle with geometry similar to 17 x 17 PWR fuel assemblies, to the results of earlier experiments, which used an FRS bundle with geometry similar to 15 x 15 PWR fuel assemblies, revealed no differences that can be attributed to the difference in geometries.

  3. Protective effects of pulmonary epithelial lining fluid on oxidative stress and DNA single-strand breaks caused by ultrafine carbon black, ferrous sulphate and organic extract of diesel exhaust particles

    SciTech Connect (OSTI)

    Chuang, Hsiao-Chi; Cheng, Yi-Ling; Lei, Yu-Chen; Chang, Hui-Hsien; Cheng, Tsun-Jen

    2013-02-01

    Pulmonary epithelial lining fluid (ELF) is the first substance to make contact with inhaled particulate matter (PM) and interacts chemically with PM components. The objective of this study was to determine the role of ELF in oxidative stress, DNA damage and the production of proinflammatory cytokines following physicochemical exposure to PM. Ultrafine carbon black (ufCB, 15 nm; a model carbonaceous core), ferrous sulphate (FeSO{sub 4}; a model transition metal) and a diesel exhaust particle (DEP) extract (a model organic compound) were used to examine the acellular oxidative potential of synthetic ELF and non-ELF systems. We compared the effects of exposure to ufCB, FeSO{sub 4} and DEP extract on human alveolar epithelial Type II (A549) cells to determine the levels of oxidative stress, DNA single-strand breaks and interleukin-8 (IL-8) production in ELF and non-ELF systems. The effects of ufCB and FeSO{sub 4} on the acellular oxidative potential, cellular oxidative stress and DNA single-strand breakage were mitigated significantly by the addition of ELF, whereas there was no decrease following treatment with the DEP extract. There was no significant effect on IL-8 production following exposure to samples that were suspended in ELF/non-ELF systems. The results of the present study indicate that ELF plays an important role in the initial defence against PM in the pulmonary environment. Experimental components, such as ufCB and FeSO{sub 4}, induced the production of oxidative stress and led to DNA single-strand breaks, which were moderately prevented by the addition of ELF. These findings suggest that ELF plays a protective role against PM-driven oxidative stress and DNA damage. -- Highlights: ► To determine the role of ELF in ROS, DNA damage and IL-8 after exposure to PM. ► ufCB, FeSO{sub 4} and DEP extract were used to examine the protective effects of ELF. ► PM-driven oxidative stress and DNA single-strand breakage were mitigated by ELF. ► The findings

  4. Nonenzymatic Role for WRN in Preserving Nascent DNA Strands after Replication Stress

    SciTech Connect (OSTI)

    Su, Fengtao; Mukherjee, Shibani; Yang, Yanyong; Mori, Eiichiro; Bhattacharya, Souparno; Kobayashi, Junya; Yannone, Steven  M.; Chen, David  J.; Asaithamby, Aroumougame

    2014-11-20

    WRN, the protein defective in Werner syndrome (WS), is a multifunctional nuclease involved in DNA damage repair, replication, and genome stability maintenance. It was assumed that the nuclease activities of WRN were critical for these functions. Here, we report a nonenzymatic role for WRN in preserving nascent DNA strands following replication stress. We found that lack of WRN led to shortening of nascent DNA strands after replication stress. Furthermore, we discovered that the exonuclease activity of MRE11 was responsible for the shortening of newly replicated DNA in the absence of WRN. Mechanistically, the N-terminal FHA domain of NBS1 recruits WRN to replication-associated DNA double-stranded breaks to stabilize Rad51 and to limit the nuclease activity of its C-terminal binding partner MRE11. Thus, this previously unrecognized nonenzymatic function of WRN in the stabilization of nascent DNA strands sheds light on the molecular reason for the origin of genome instability in WS individuals.

  5. MicroRNAs form triplexes with double stranded DNA at sequence-specific binding sites; a eukaryotic mechanism via which microRNAs could directly alter gene expression

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Paugh, Steven W.; Coss, David R.; Bao, Ju; Laudermilk, Lucas T.; Grace, Christy R.; Ferreira, Antonio M.; Waddell, M. Brett; Ridout, Granger; Naeve, Deanna; Leuze, Michael Rex; et al

    2016-02-04

    MicroRNAs are important regulators of gene expression, acting primarily by binding to sequence-specific locations on already transcribed messenger RNAs (mRNA). Recent studies indicate that microRNAs may also play a role in up-regulating mRNA transcription levels, although a definitive mechanism has not been established. Double-helical DNA is capable of forming triple-helical structures through Hoogsteen and reverse Hoogsteen interactions in the major groove of the duplex, and we show physical evidence that microRNAs form triple-helical structures with duplex DNA, and identify microRNA sequences that favor triplex formation. We developed an algorithm (Trident) to search genome-wide for potential triplex-forming sites and show thatmore » several mammalian and non-mammalian genomes are enriched for strong microRNA triplex binding sites. We show that those genes containing sequences favoring microRNA triplex formation are markedly enriched (3.3 fold, p<2.2 x 10-16) for genes whose expression is positively correlated with expression of microRNAs targeting triplex binding sequences. As a result, this work has thus revealed a new mechanism by which microRNAs can interact with gene promoter regions to modify gene transcription.« less

  6. Nonenzymatic Role for WRN in Preserving Nascent DNA Strands after Replication Stress

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Su, Fengtao; Mukherjee, Shibani; Yang, Yanyong; Mori, Eiichiro; Bhattacharya, Souparno; Kobayashi, Junya; Yannone, Steven  M.; Chen, David  J.; Asaithamby, Aroumougame

    2014-11-20

    WRN, the protein defective in Werner syndrome (WS), is a multifunctional nuclease involved in DNA damage repair, replication, and genome stability maintenance. It was assumed that the nuclease activities of WRN were critical for these functions. Here, we report a nonenzymatic role for WRN in preserving nascent DNA strands following replication stress. We found that lack of WRN led to shortening of nascent DNA strands after replication stress. Furthermore, we discovered that the exonuclease activity of MRE11 was responsible for the shortening of newly replicated DNA in the absence of WRN. Mechanistically, the N-terminal FHA domain of NBS1 recruits WRNmore » to replication-associated DNA double-stranded breaks to stabilize Rad51 and to limit the nuclease activity of its C-terminal binding partner MRE11. Thus, this previously unrecognized nonenzymatic function of WRN in the stabilization of nascent DNA strands sheds light on the molecular reason for the origin of genome instability in WS individuals.« less

  7. BREAKING BOND

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    BREAKING BOND the Can chemistry help unbind us from fossil fuels? 1663 July 2016 21 When ... Their goal: to use chemistry to construct gasoline-like hydrocarbons from plant sugars. ...

  8. Method for producing labeled single-stranded nucleic acid probes

    DOE Patents [OSTI]

    Dunn, John J.; Quesada, Mark A.; Randesi, Matthew

    1999-10-19

    Disclosed is a method for the introduction of unidirectional deletions in a cloned DNA segment. More specifically, the method comprises providing a recombinant DNA construct comprising a DNA segment of interest inserted in a cloning vector, the cloning vector having an f1 endonuclease recognition sequence adjacent to the insertion site of the DNA segment of interest. The recombinant DNA construct is then contacted with the protein pII encoded by gene II of phage f1 thereby generating a single-stranded nick. The nicked DNA is then contacted with E. coli Exonuclease III thereby expanding the single-stranded nick into a single-stranded gap. The single-stranded gapped DNA is then contacted with a single-strand-specific endonuclease thereby producing a linearized DNA molecule containing a double-stranded deletion corresponding in size to the single-stranded gap. The DNA treated in this manner is then incubated with DNA ligase under conditions appropriate for ligation. Also disclosed is a method for producing single-stranded DNA probes. In this embodiment, single-stranded gapped DNA, produced as described above, is contacted with a DNA polymerase in the presence of labeled nucleotides to fill in the gap. This DNA is then linearized by digestion with a restriction enzyme which cuts outside the DNA segment of interest. The product of this digestion is then denatured to produce a labeled single-stranded nucleic acid probe.

  9. Pair breaking versus symmetry breaking: Origin of the Raman modes...

    Office of Scientific and Technical Information (OSTI)

    Pair breaking versus symmetry breaking: Origin of the Raman modes in superconducting cuprates Citation Details In-Document Search Title: Pair breaking versus symmetry breaking:...

  10. Double hull grounding experiments

    SciTech Connect (OSTI)

    Rodd, J.L.; Sikora, J.P.

    1995-12-31

    In the last few years the public and governments of many nations have become increasingly aware of the need for improving oil tanker safety. The requirements for double hull tankers are an attempt to address this need through legislation. Even though a number of investigations on the mechanics of collisions have been done in the past, until recently very little research supported the development of structural improvements to reduce oil tanker damage during grounding and stranding accidents. An aggressive evaluation of double hull tanker crashworthiness in stranding and grounding accidents is underway at CD/NSWC (formerly the David Taylor Research Center). The ability to predict damage from grounding accidents accurately is not currently available. The objective of this paper is to present qualitatively the structural failure mechanisms associated with stranding and grounding events for candidate double hull tanker structures and to present some simple methods for comparing damage scenarios. A comparison of the structural performance of key features in several very different designs will provide useful information toward this understanding.

  11. Breaking the Bond

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Breaking the Bond 1663 Los Alamos science and technology magazine Latest Issue:July 2016 past issues All Issues » submit Breaking the Bond Can chemistry help unbind us from fossil fuels? July 21, 2016 info graphic gas pump Accelerating toward a more sustainable future: gas pumps may someday soon contain bio-gasoline made from plants. We are condensing millions of years of fossilization into a few chemical reactions. Fossil fuels are a finite resource. Unfortunately, the existing global

  12. Neutrino Physics AAPT Strand Day

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Physics AAPT Strand Day NSTA Regional, 2005 Jocelyn Monroe, Columbia University 1. What Is a Neutrino Anyway? 2. The Question Of Neutrino Mass 3. Searching For Neutrino Oscillations 4. Where Are We Now? ν Neutrinos, they are very small. They have no charge and have no mass And do not interact at all. The earth is just a silly ball To them, through which they simply pass... ...And pierce the lover and his lass From underneath the bed- you call It wonderful; I call it crass. J from ``Cosmic

  13. Minimal Doubling and Point Splitting

    SciTech Connect (OSTI)

    Creutz, M.

    2010-06-14

    Minimally-doubled chiral fermions have the unusual property of a single local field creating two fermionic species. Spreading the field over hypercubes allows construction of combinations that isolate specific modes. Combining these fields into bilinears produces meson fields of specific quantum numbers. Minimally-doubled fermion actions present the possibility of fast simulations while maintaining one exact chiral symmetry. They do, however, introduce some peculiar aspects. An explicit breaking of hyper-cubic symmetry allows additional counter-terms to appear in the renormalization. While a single field creates two different species, spreading this field over nearby sites allows isolation of specific states and the construction of physical meson operators. Finally, lattice artifacts break isospin and give two of the three pseudoscalar mesons an additional contribution to their mass. Depending on the sign of this mass splitting, one can either have a traditional Goldstone pseudoscalar meson or a parity breaking Aoki-like phase.

  14. After Cajun, what next for stranded costs?

    SciTech Connect (OSTI)

    Pembroke, J.D.

    1994-10-01

    Members of FERC have said that the Cajun decision does not pose a serious problem to its proposed rulemaking on stranded costs. But the D.C. Circuit`s Cajun decision, criticizing the concept of recovery for stranded investment in broad terms, promises to make that task difficult, if not impossible. The United States Court of Appeals for the District of Columbia Circuit, in its July 12, 1994 opinion in Cajun Electric Power Cooperative, Inc. v. Federal Energy Regulatory Commission, subjected the Entergy Corporations`s transmission tariff`s stranded investment provision to broad and, perhaps, fatal criticism. The Cajun opinion, premised on basic concepts of antitrust law, was issued at a time of substantial industry discussion on the concept of stranded investment and should greatly impact the decisions of both federal and state regulators on stranded investment.

  15. Metallographic autopsies of full-scale ITER prototype cable-in-conduit conductors after full cyclic testing in SULTAN: II. Significant reduction of strand movement and strand damage in short twist pitch CICCs

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Sanabria, Charlos; Lee, Peter J.; Starch, William; Devred, Arnaud; Larbalestier, David C.

    2015-10-14

    Prototype cable in conduit conductors (CICCs) destined for use in the Toroidal Field (TF) and Central Solenoid (CS) coils of the ITER experimental fusion reactor underwent severe cyclic loading in the SULTAN facility. Their autopsies revealed significant and permanent transverse strand migration due to the large Lorentz forces of the SULTAN test. The movement resulted in a 3 7% void fraction increase on the Low Pressure (LP) side of the longer twist pitch CICCs. However, short twist pitch conductors exhibited less than 1% void fraction increase in the LP side, as well as a complete absence of the Nb3Sn filamentmore » fractures observed in the longer twist pitch conductors. We report here a detailed strand to cable analysis of short and longer “baseline” twist pitch CICCs. It was found that the use of Internal Tin strands in the longer “baseline” twist pitch CICCs can be beneficial possibly because of their superior stiffness—which better resist strand movement—while the use of Bronze Process strands showed more movement and poorer cyclic test performance. This was not the case for the short twist pitch CICC. Such conductor design seems to work well with both strand types. But it was found that despite the absence of filament fractures, the short twist pitch CICC made from the Internal Tin strands studied here developed severe strand distortion during cabling which resulted in diffusion barrier breaks and Sn contamination of the Cu stabilizer during the heat treatment. Furthermore, the short twist pitch CICC made from Bronze Process strands preserved diffusion barrier integrity.« less

  16. Titanium subhydride potassium perchlorate (TiH1.65/KClO4) burn rates from hybrid closed bomb-strand burner experiments.

    SciTech Connect (OSTI)

    Cooper, Marcia A.; Oliver, Michael S.

    2012-08-01

    A hybrid closed bomb-strand burner is used to measure the burning behavior of the titanium subhydride potassium perchlorate pyrotechnic with an equivalent hydrogen concentration of 1.65. This experimental facility allows for simultaneous measurement of the closed bomb pressure rise and pyrotechnic burn rate as detected by electrical break wires over a range of pressures. Strands were formed by pressing the pyrotechnic powders to bulk densities between 60% and 90% theoretical maximum density. The burn rate dependance on initial density and vessel pressure are measured. At all initial strand densities, the burn is observed to transition from conductive to convective burning within the strand. The measured vessel pressure history is further analyzed following the closed bomb analysis methods developed for solid propellants.

  17. Pair extended coupled cluster doubles

    SciTech Connect (OSTI)

    Henderson, Thomas M.; Scuseria, Gustavo E.; Bulik, Ireneusz W.

    2015-06-07

    The accurate and efficient description of strongly correlated systems remains an important challenge for computational methods. Doubly occupied configuration interaction (DOCI), in which all electrons are paired and no correlations which break these pairs are permitted, can in many cases provide an accurate account of strong correlations, albeit at combinatorial computational cost. Recently, there has been significant interest in a method we refer to as pair coupled cluster doubles (pCCD), a variant of coupled cluster doubles in which the electrons are paired. This is simply because pCCD provides energies nearly identical to those of DOCI, but at mean-field computational cost (disregarding the cost of the two-electron integral transformation). Here, we introduce the more complete pair extended coupled cluster doubles (pECCD) approach which, like pCCD, has mean-field cost and reproduces DOCI energetically. We show that unlike pCCD, pECCD also reproduces the DOCI wave function with high accuracy. Moreover, pECCD yields sensible albeit inexact results even for attractive interactions where pCCD breaks down.

  18. Method and apparatus for testing a forward-moving strand

    DOE Patents [OSTI]

    Ducommun, Joel; Vulliens, Philippe

    1980-01-01

    In a method for testing a continuously forward-moving strand a light beam which passes along a plane that extends approximately perpendicularly to the longitudinal axis of the strand is introduced into the strand. The brightness value is measured on a place of the strand exterior which is distal from the light incidence place by means of at least one photoelectronic element disposed directly on the strand exterior and the measured result is evaluated in a gating circuit which is electrically connected to the photoelectronic element.

  19. DNB CP - Steamline Break Analysis

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    5-000 DNB Challenge Problem Progress - Steamline Break Analysis Industry Council Meeting April 12, 2016 2 CASL-U-2016-1085-000 Outline * DNB CP to Meet Industry's Needs * PWR Steamline Break (SLB) Analysis Overview * Ongoing FY16 Activities on SLB Analysis 3 CASL-U-2016-1085-000 Departure from Nucleate Boiling (DNB) * DNB also referred to as Critical Heat Flux (CHF) - Local clad surface dryout causes dramatic reduction in heat transfer during transients - One of safety and regulatory acceptance

  20. Methods to estimate stranded commitments for a restructuring US electricity industry

    SciTech Connect (OSTI)

    Hirst, E.; Hadley, S.; Baxter, L.

    1996-01-01

    Estimates of stranded commitments for US investor-owned electric utilities range widely, from as little as $20 billion to as much as $500 billion (more than double the shareholder equity in US utilities). These potential losses are a consequence of the above-market book values for some utility-owned power plants, long-term power-purchase contracts, deferred income taxes, regulatory assets, and public-policy programs. Because of the wide range of estimates and the potentially large dollar amounts involved, state and federal regulators need a clear understanding of the methods used to calculate these estimates. In addition, they may want simple methods that they can use to check the reasonableness of the estimates that utilities and other parties present in regulatory proceedings. This report explains various top-down and bottom-up methods to calculate stranded commitments. The purpose of this analysis is to help regulators and others understand the implications of different analytical approaches to estimating stranded-commitment amounts. Top-down methods, because they use the utility as the unit of analysis, are simple to apply and to understand. However, their aggregate nature makes it difficult to determine what specific assets and liabilities affect their estimates. Bottom-up methods use the individual asset (e.g., power plant) or liability (e.g., power-purchase contract, fuel-supply contract, and deferred income taxes) as the unit of analysis. These methods have substantial data and computational requirements.

  1. Breaking the Biological Barriers to Cellulosic Ethanol, June...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Breaking the Biological Barriers to Cellulosic Ethanol, June 2006 Breaking the Biological Barriers to Cellulosic Ethanol, June 2006 Breaking the Biological Barriers to Cellulosic ...

  2. Give Young Scientists a Break

    SciTech Connect (OSTI)

    Wiley, H. S.

    2009-11-01

    There has been much concern about the impact of tight funding on the careers of young scientists. When only a small percentage of grants are approved, even the smallest problem or error with an application can push it out of the funding range. Unfortunately, the relative lack of grant writing skills by new investigators often has this effect. To avoid a situation where only experienced investigators with polished writing skills are funded, the National Institutes of Health has instituted a more generous ranking scale for new investigators. Not surprisingly, some senior investigators have protested, calling it reverse discrimination. I say that their anger is misplaced. New investigators do deserve a break.

  3. Total-derivative supersymmetry breaking

    SciTech Connect (OSTI)

    Haba, Naoyuki; Uekusa, Nobuhiro

    2010-05-15

    On an interval compactification in supersymmetric theory, boundary conditions for bulk fields must be treated carefully. If they are taken arbitrarily following the requirement that a theory is supersymmetric, the conditions could give redundant constraints on the theory. We construct a supersymmetric action integral on an interval by introducing brane interactions with which total-derivative terms under the supersymmetry transformation become zero due to a cancellation. The variational principle leads equations of motion and also boundary conditions for bulk fields, which determine boundary values of bulk fields. By estimating mass spectrum, spontaneous supersymmetry breaking in this simple setup can be realized in a new framework. This supersymmetry breaking does not induce a massless R axion, which is favorable for phenomenology. It is worth noting that fermions in hyper-multiplet, gauge bosons, and the fifth-dimensional component of gauge bosons can have zero-modes (while the other components are all massive as Kaluza-Klein modes), which fits the gauge-Higgs unification scenarios.

  4. NREL: Technology Transfer - Popular Mechanics: Scientists Break...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Popular Mechanics: Scientists Break This Virtual Power Grid to Save the Real One July 27, 2015 Popular Mechanics describes how NREL's Energy Systems Integration Facility (ESIF)...

  5. new chemistry to break down cell walls

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    new chemistry to break down cell walls - Sandia Energy Energy Search Icon Sandia Home ... Stationary Power Energy Conversion Efficiency Solar Energy Wind Energy Water Power ...

  6. Vanadium catalysts break down biomass for fuels

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    break down biomass into useful components Due to diminishing petroleum reserves, non-food biomass (lignocellulose) is an attractive alternative as a feedstock for the...

  7. Dyes designed for high sensitivity detection of double-stranded DNA

    DOE Patents [OSTI]

    Glazer, Alexander N.; Benson, Scott C.

    1998-01-01

    Novel fluorescent dyes are provided, characterized by having a fluorophore joined to a cationic chain. The dyes are found to provide for high enhancement upon binding to nucleic acid and have strong binding affinities to the nucleic acid, as compared to the fluorophore without the polycationic chain. The dyes find use in detection of dsDNA in gel electrophoresis and solution at substantially higher sensitivities using substantially less dye.

  8. Dyes designed for high sensitivity detection of double-stranded DNA

    DOE Patents [OSTI]

    Glazer, A.N.; Benson, S.C.

    1998-07-21

    Novel fluorescent dyes are provided, characterized by having a fluorophore joined to a cationic chain. The dyes are found to provide for high enhancement upon binding to nucleic acid and have strong binding affinities to the nucleic acid, as compared to the fluorophore without the polycationic chain. The dyes find use in detection of dsDNA in gel electrophoresis and solution at substantially higher sensitivities using substantially less dye. 10 figs.

  9. Dyes designed for high sensitivity detection of double-stranded DNA

    DOE Patents [OSTI]

    Glazer, Alexander N.; Benson, Scott C.

    2000-01-01

    Novel fluorescent dyes are provided, characterized by having a fluorophore joined to a cationic chain. The dyes are found to provide for high enhancement upon binding to nucleic acid and have strong binding affinities to the nucleic acid, as compared to the fluorophore without the polycationic chain. The dyes find use in detection of dsDNA in gel electrophoresis and solution at substantially higher sensitivities using substantially less dye.

  10. Dyes designed for high sensitivity detection of double-stranded DNA

    DOE Patents [OSTI]

    Glazer, Alexander N.; Benson, Scott C.

    1994-01-01

    Novel fluorescent dyes are provided, characterized by having a fluorophore joined to a polycationic chain of at least two positive charges. The dyes are found to provide for high enhancement upon binding to nucleic acid and have strong binding affinities to the nucleic acid, as compared to the fluorophore without the polycationic chain. The dyes find use in detection of dsDNA in gel electrophoresis and solution at substantially higher sensitivities using substantially less dye.

  11. The concepts of leak before break and absolute reliability of NPP equipment and piping

    SciTech Connect (OSTI)

    Getman, A.F.; Komarov, O.V.; Sokov, L.M.

    1997-04-01

    This paper describes the absolute reliability (AR) concept for ensuring safe operation of nuclear plant equipment and piping. The AR of a pipeline or component is defined as the level of reliability when the probability of an instantaneous double-ended break is near zero. AR analysis has been applied to Russian RBMK and VVER type reactors. It is proposed that analyses required for application of the leak before break concept should be included in AR implementation. The basic principles, methods, and approaches that provide the basis for implementing the AR concept are described.

  12. Background meeting and technology demonstration: Chromium electroplating of superconductor strand

    SciTech Connect (OSTI)

    1995-05-01

    A meeting concerned with electroplating copper onto superconducting wires was held. Topics of discussion were concerned with the market, strand cleanliness, and an improved rinsing system for the process.

  13. Unidirectional Scaffold-Strand Arrangement in DNA Origami

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Unidirectional Scaffold-Strand Arrangement in DNA Origami Authors: Han, D., Jiang, S., Samanta, A., Liu, Y., and Yan, H. Title: Unidirectional Scaffold-Strand Arrangement in DNA Origami Source: Angewandte Chemie International Edition Year: 2013 Volume: 52 Pages: 9031-9034 ABSTRACT: Date of online publication: Sun, 2013-07-14 Link online: http://onlinelibrary.wiley.com/doi/10.1002/anie.201302177/suppinfo

  14. A double-double/double-single computation package

    Energy Science and Technology Software Center (OSTI)

    2004-12-01

    The DDFUNIDSFUN software permits a new or existing Fortran-90 program to utilize double-double precision (approx. 31 digits) or double-single precision (approx. 14 digits) arithmetic. Double-double precision is required by a rapidly expandirtg body of scientific computations in physics and mathematics, for which the conventional 64-bit IEEE computer arithmetic (about 16 decimal digit accuracy) is not sufficient. Double-single precision permits users of systems that do not have hardware 64-bit IEEE arithmetic (such as some game systems)more » to perform arithmetic at a precision nearly as high as that of systems that do. Both packages run significantly faster Than using multiple precision or arbitrary precision software for this purpose. The package includes an extensive set of low-level routines to perform high-precision arithmetic, including routines to calculate various algebraic and transcendental functions, such as square roots, sin, ccc, exp, log and others. In addition, the package includes high-level translation facilities, so that Fortran programs can utilize these facilities by making only a few changes to conventional Fortran programs. In most cases, the only changes that are required are to change the type statements of variables that one wishes to be treated as multiple precision, plus a few other minor changes. The DDFUN package is similar in functionality to the double-double part of the GD package, which was previously written at LBNL. However, the DDFUN package is written exclusively in Fortran-90, thus avoidIng difficulties that some users experience when using GD, which includes both Fortran-90 and C++ code.« less

  15. Chromosome doubling method

    DOE Patents [OSTI]

    Kato, Akio

    2006-11-14

    The invention provides methods for chromosome doubling in plants. The technique overcomes the low yields of doubled progeny associated with the use of prior techniques for doubling chromosomes in plants such as grasses. The technique can be used in large scale applications and has been demonstrated to be highly effective in maize. Following treatment in accordance with the invention, plants remain amenable to self fertilization, thereby allowing the efficient isolation of doubled progeny plants.

  16. An intercalation-locked parallel-stranded DNA tetraplex

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Tripathi, S.; Zhang, D.; Paukstelis, P. J.

    2015-01-27

    DNA has proved to be an excellent material for nanoscale construction because complementary DNA duplexes are programmable and structurally predictable. However, in the absence of Watson–Crick pairings, DNA can be structurally more diverse. Here, we describe the crystal structures of d(ACTCGGATGAT) and the brominated derivative, d(ACBrUCGGABrUGAT). These oligonucleotides form parallel-stranded duplexes with a crystallographically equivalent strand, resulting in the first examples of DNA crystal structures that contains four different symmetric homo base pairs. Two of the parallel-stranded duplexes are coaxially stacked in opposite directions and locked together to form a tetraplex through intercalation of the 5'-most A–A base pairs betweenmore » adjacent G–G pairs in the partner duplex. The intercalation region is a new type of DNA tertiary structural motif with similarities to the i-motif. 1H–1H nuclear magnetic resonance and native gel electrophoresis confirmed the formation of a parallel-stranded duplex in solution. Finally, we modified specific nucleotide positions and added d(GAY) motifs to oligonucleotides and were readily able to obtain similar crystals. This suggests that this parallel-stranded DNA structure may be useful in the rational design of DNA crystals and nanostructures.« less

  17. An intercalation-locked parallel-stranded DNA tetraplex

    SciTech Connect (OSTI)

    Tripathi, S.; Zhang, D.; Paukstelis, P. J.

    2015-01-27

    DNA has proved to be an excellent material for nanoscale construction because complementary DNA duplexes are programmable and structurally predictable. However, in the absence of Watson–Crick pairings, DNA can be structurally more diverse. Here, we describe the crystal structures of d(ACTCGGATGAT) and the brominated derivative, d(ACBrUCGGABrUGAT). These oligonucleotides form parallel-stranded duplexes with a crystallographically equivalent strand, resulting in the first examples of DNA crystal structures that contains four different symmetric homo base pairs. Two of the parallel-stranded duplexes are coaxially stacked in opposite directions and locked together to form a tetraplex through intercalation of the 5'-most A–A base pairs between adjacent G–G pairs in the partner duplex. The intercalation region is a new type of DNA tertiary structural motif with similarities to the i-motif. 1H–1H nuclear magnetic resonance and native gel electrophoresis confirmed the formation of a parallel-stranded duplex in solution. Finally, we modified specific nucleotide positions and added d(GAY) motifs to oligonucleotides and were readily able to obtain similar crystals. This suggests that this parallel-stranded DNA structure may be useful in the rational design of DNA crystals and nanostructures.

  18. A Fullerene that Breaks the Rules

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    A Fullerene that Breaks the Rules A Fullerene that Breaks the Rules Print Tuesday, 28 April 2015 00:00 In the 30 years since the discovery of the classic fullerene "buckyball" of 60 carbon atoms, scientists have found many variations on the fullerene theme: carbon nanotubes (elongated buckyballs), endohedral fullerenes (buckyballs with molecules inside), and even carbon "peapods" (nanotubes with buckyballs inside). Within this great diversity, however, certain rules emerged.

  19. Factors that affect electric-utility stranded commitments

    SciTech Connect (OSTI)

    Hirst, E.; Hadley, S.; Baxter, L.

    1996-07-01

    Estimates of stranded commitments for U.S. investor-owned utilities range widely, with many falling in the range of $100 to $200 billion. These potential losses exist because some utility-owned power plants, long-term power-purchase contracts and fuel-supply contracts, regulatory assets, and expenses for public-policy programs have book values that exceed their expected market values under full competition. This report quantifies the sensitivity of stranded- commitment estimates to the various factors that lead to these above- market-value estimates. The purpose of these sensitivity analyses is to improve understanding on the part of state and federal regulators, utilities, customers, and other electric-industry participants about the relative importance of the factors that affect stranded- commitment amounts.

  20. Bending-induced Symmetry Breaking of Lithiation in Germanium...

    Office of Scientific and Technical Information (OSTI)

    Bending-induced Symmetry Breaking of Lithiation in Germanium Nanowires Citation Details In-Document Search Title: Bending-induced Symmetry Breaking of Lithiation in Germanium ...

  1. Novel Thermal Break with Simplified Manufacturing for R7 Commercial...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Thermal Break with Simplified Manufacturing for R7 Commercial Windows Novel Thermal Break with Simplified Manufacturing for R7 Commercial Windows Lead Performer: Alcoa - ...

  2. Break-out Discussion i: Modeling Consumer Behavior Residential...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Break-out Discussion i: Modeling Consumer Behavior Residential Scale Break-out Discussion i: Modeling Consumer Behavior Residential Scale This presentaion summarizes the ...

  3. Double Flash | Open Energy Information

    Open Energy Info (EERE)

    Double Flash Jump to: navigation, search Retrieved from "http:en.openei.orgwindex.php?titleDoubleFlash&oldid599606...

  4. Method for fabricating multi-strand superconducting cable

    DOE Patents [OSTI]

    Borden, A.R.

    1985-04-01

    Multi-strand superconducting cables adapted to be used, for example, to wind a magnet are fabricated by directing wire strands inwardly from spools disposed on the perimeter of a rotating disk and wrapping them diagonally around a tapered mandrel with a flattened cross-sectional shape with a core having a wedge-shaped channel. As the cable is pulled axially, flexibly coupled wedge-shaped pieces are continuously passed through the channel in the mandrel and inserted into the cable as an internal support therefor.

  5. Applications of Strand-Specific in situ Hybridization

    SciTech Connect (OSTI)

    Goodwin, E.H.; Meyne, J.; Bailey, S.M.; Quigley, D.; Smith, L.; Tennyson, R.

    1997-01-01

    This is the final report of a three-year, Laboratory-Directed Research and Development (LDRD) project at the Los Alamos National Laboratory (LANL). Fluorescence in situ hybridization (FISH) is used to determine the location of specific DNA sequences on chromosomes. It is an effective tool in genomic mapping and is finding increasing use in medical diagnosis. A ''strand-specific'' version of FISH has been developed in the Life Sciences Division of LANL. The new procedure, named CO-FISH, reveals not only location but also the 5'-to-3'direction of a target sequence, such as the sense strand of a gene. This project was designed to investigate applications of the new technique. Strand-specific FISH was found to be useful and informative for genomic mapping of repetitive DNA sequences. The method provide a valuable new tool for investigating the mechanisms of aneuploidy inducing agents and the cytogenetic phenomena called lateral asymmetry. Finally, using strand-specific FISH, the authors were able to detect certain types of chromosome aberrations (isochromosomes, inversions and Robertsonian translocations) that can be difficult to observe with standard techniques.

  6. Chiral symmetry and chiral-symmetry breaking

    SciTech Connect (OSTI)

    Peskin, M.E.

    1982-12-01

    These lectures concern the dynamics of fermions in strong interaction with gauge fields. Systems of fermions coupled by gauge forces have a very rich structure of global symmetries, which are called chiral symmetries. These lectures will focus on the realization of chiral symmetries and the causes and consequences of thier spontaneous breaking. A brief introduction to the basic formalism and concepts of chiral symmetry breaking is given, then some explicit calculations of chiral symmetry breaking in gauge theories are given, treating first parity-invariant and then chiral models. These calculations are meant to be illustrative rather than accurate; they make use of unjustified mathematical approximations which serve to make the physics more clear. Some formal constraints on chiral symmetry breaking are discussed which illuminate and extend the results of our more explicit analysis. Finally, a brief review of the phenomenological theory of chiral symmetry breaking is presented, and some applications of this theory to problems in weak-interaction physics are discussed. (WHK)

  7. Workshop on electroweak symmetry breaking: proceedings

    SciTech Connect (OSTI)

    Hinchliffe, I.

    1984-10-01

    A theoretical workshop on electroweak symmetry breaking at the Superconducting Supercollider was held at Lawrence Berkeley Laboratory, June 4-22, 1984. The purpose of the workshop was to focus theoretical attention on the ways in which experimentation at the SSC could reveal manifestations of the phenomenon responsible for electroweak symmetry breaking. This issue represents, at present, the most compelling scientific argument for the need to explore the energy region to be made accessible by the SSC, and a major aim of the workshop was to involve a broad cross section of particle theorists in the ongoing process of sharpening the requirements for both accelerator and detector design that will ensure detection and identification of meaningful signals, whatever form the electroweak symmetry breaking phenomenon should actually take. Separate entries were prepared for the data base for the papers presented.

  8. Entropic information for travelling solitons in Lorentz and CPT breaking systems

    SciTech Connect (OSTI)

    Correa, R.A.C.; Rocha, Roldão da; Souza Dutra, A. de

    2015-08-15

    In this work we group four research topics apparently disconnected, namely solitons, Lorentz symmetry breaking, supersymmetry, and entropy. Following a recent work (Gleiser and Stamatopoulos, 2012), we show that it is possible to construct in the context of travelling wave solutions a configurational entropy measure in functional space, from the field configurations. Thus, we investigate the existence and properties of travelling solitons in Lorentz and CPT breaking scenarios for a class of models with two interacting scalar fields. Here, we obtain a complete set of exact solutions for the model studied which display both double and single-kink configurations. In fact, such models are very important in applications that include Bloch branes, Skyrmions, Yang–Mills, Q-balls, oscillons and various superstring-motivated theories. We find that the so-called Configurational Entropy (CE) for travelling solitons shows that the best value of parameter responsible to break the Lorentz symmetry is one where the energy density is distributed equally around the origin. In this way, the information-theoretical measure of travelling solitons in Lorentz symmetry violation scenarios opens a new window to probe situations where the parameters responsible for breaking the symmetries are arbitrary. In this case, the CE selects the best value of the parameter in the model.

  9. Improvements in strand feeding and its effect of sintering performance

    SciTech Connect (OSTI)

    Beer, H.; Kersting, K.; Werner, P.

    1995-12-01

    Sintering may be considered a rather simple, counter current gas-solid process. A bed of granular solids is moved horizontally on a strand of pallets and suction is applied beneath the grate. Shortly after the sinter mix is fed onto the strand the incorporated solid fuel is ignited in the surface layer and the hot gases are drawn into the bed. The temperature of the top layer is raised high enough to burn the fuel particles while air is sucked down through it. Passing the upper, already sintered part of the bed the air is first preheated then sustains the combustion reaction. The hot, still oxygen-rich combustion gases leave the sintering zone and transfer its heat to the charge below. While the solids are preheated, carbonates, combined water, and moisture are driven off, rapidly cooling the gas. Thus, a flame front propagates through the traveling bed, generating at peak temperatures enough heat to agglomerate the bed of quasi-particles into a sinter cake. The strand speed is adjusted so that the burning through of the combustion zone coincides with the end of the suction area. To ensure stable operation this cross stream reactor has to be kept in a steady state.

  10. A Fullerene that Breaks the Rules

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    A Fullerene that Breaks the Rules Print In the 30 years since the discovery of the classic fullerene "buckyball" of 60 carbon atoms, scientists have found many variations on the fullerene theme: carbon nanotubes (elongated buckyballs), endohedral fullerenes (buckyballs with molecules inside), and even carbon "peapods" (nanotubes with buckyballs inside). Within this great diversity, however, certain rules emerged. One such rule governed the number and placement of the

  11. A Fullerene that Breaks the Rules

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    A Fullerene that Breaks the Rules Print In the 30 years since the discovery of the classic fullerene "buckyball" of 60 carbon atoms, scientists have found many variations on the fullerene theme: carbon nanotubes (elongated buckyballs), endohedral fullerenes (buckyballs with molecules inside), and even carbon "peapods" (nanotubes with buckyballs inside). Within this great diversity, however, certain rules emerged. One such rule governed the number and placement of the

  12. A Fullerene that Breaks the Rules

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    A Fullerene that Breaks the Rules Print In the 30 years since the discovery of the classic fullerene "buckyball" of 60 carbon atoms, scientists have found many variations on the fullerene theme: carbon nanotubes (elongated buckyballs), endohedral fullerenes (buckyballs with molecules inside), and even carbon "peapods" (nanotubes with buckyballs inside). Within this great diversity, however, certain rules emerged. One such rule governed the number and placement of the

  13. A Fullerene that Breaks the Rules

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    A Fullerene that Breaks the Rules Print In the 30 years since the discovery of the classic fullerene "buckyball" of 60 carbon atoms, scientists have found many variations on the fullerene theme: carbon nanotubes (elongated buckyballs), endohedral fullerenes (buckyballs with molecules inside), and even carbon "peapods" (nanotubes with buckyballs inside). Within this great diversity, however, certain rules emerged. One such rule governed the number and placement of the

  14. Electroweak Symmetry Breaking in Historical Perspective

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Quigg, Chris

    2015-10-01

    The discovery of the Higgs boson is a major milestone in our progress toward understanding the natural world. A particular aim of my review is to show how diverse ideas came together in the conception of electroweak symmetry breaking that led up to the discovery. Furthermore, I survey what we know now that we did not know before, what properties of the Higgs boson remain to be established, and what new questions we may now hope to address.

  15. Textural break foundation wall construction modules

    DOE Patents [OSTI]

    Phillips, Steven J.

    1990-01-01

    Below-grade, textural-break foundation wall structures are provided for inhibiting diffusion and advection of liquids and gases into and out from a surrounding hydrogeologic environment. The foundation wall structure includes a foundation wall having an interior and exterior surface and a porous medium disposed around a portion of the exterior surface. The structure further includes a modular barrier disposed around a portion of the porous medium. The modular barrier is substantially removable from the hydrogeologic environment.

  16. Cloud Properties Working Group Break Out Session

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Break Out Session ARM Science Team Meeting Louisville, KY 30 March 2009 The Chair's Objectives for CPWG *Maintain continuity of "base" instruments - We're building a climatology! *Advocate for sufficient programmatic support to make our measurements useful. *Better retrieval vetting framework - moving towards Cloud Properties Best Estimate *Build a stronger connection with the modeling community - Producing the products they want. CPWG Breakout Agenda 30 March 2009, 3-5 pm *3:00-3:15

  17. 5-MW Dynamometer Ground Breaking | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    5-MW Dynamometer Ground Breaking 5-MW Dynamometer Ground Breaking December 19, 2011 - 3:04pm Addthis This is an excerpt from the Fourth Quarter 2011 edition of the Wind Program R&D ...

  18. Breaking a Pocket of Resistance in the Fight Against Cancer

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Breaking a Pocket of Resistance in the Fight Against Cancer Breaking a Pocket of Resistance in the Fight Against Cancer Print Thursday, 12 December 2013 11:55 ras protein The new...

  19. Modulated Tool-Path (MTP) Chip Breaking System - Energy Innovation...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    PDF Document Publication R&D 100 award (Chip Breaking)(final).pdf (8,767 KB) Technology Marketing Summary The MTP chip breaking system is a cost-saving solution for machining ...

  20. Ground Breaking of Blythe Solar Power Project | Department of...

    Energy Savers [EERE]

    Breaking of Blythe Solar Power Project Ground Breaking of Blythe Solar Power Project June 20, 2011 - 2:16pm Addthis Secretary Chu Secretary Chu Former Secretary of Energy What will ...

  1. Breaking the Biological Barriers to Cellulosic Ethanol, June 2006 |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Breaking the Biological Barriers to Cellulosic Ethanol, June 2006 Breaking the Biological Barriers to Cellulosic Ethanol, June 2006 Breaking the Biological Barriers to Cellulosic Ethanol, June 2006 b2blowres63006.pdf (8.11 MB) More Documents & Publications Breaking the Biological Barriers to Cellulosic Ethanol, June 2006 Review of Recent Pilot Scale Cellulosic Ethanol Demonstration Biochemical Conversion: Using Hydrolysis, Fermentation, and Catalysis to Make Fuels

  2. Neutrino decay and neutrinoless double beta decay in a 3-3-1 model

    SciTech Connect (OSTI)

    Dias, Alex G. [Instituto de Fisica, Universidade de Sao Paulo, Caixa Postal 66.318, 05315-970, Sao Paulo-SP (Brazil); Doff, A. [Instituto de Fisica Teorica, Universidade Estadual Paulista, Rua Pamplona 145, 01405-900 Sao Paulo-SP (Brazil); Pires, C.A. de S; Rodrigues da Silva, P.S. [Departamento de Fisica, Universidade Federal da Paraiba, Caixa Postal 5008, 58051-970, Joao Pessoa-PB (Brazil)

    2005-08-01

    In this work we show that the implementation of spontaneous breaking of the lepton number in the 3-3-1 model with right-handed neutrinos gives rise to fast neutrino decay with Majoron emission and generates a bunch of new contributions to the neutrinoless double beta decay.

  3. Breaking Barriers in Polymer Additive Manufacturing

    SciTech Connect (OSTI)

    Love, Lonnie J; Duty, Chad E; Post, Brian K; Lind, Randall F; Lloyd, Peter D; Kunc, Vlastimil; Peter, William H; Blue, Craig A

    2015-01-01

    Additive Manufacturing (AM) enables the creation of complex structures directly from a computer-aided design (CAD). There are limitations that prevent the technology from realizing its full potential. AM has been criticized for being slow and expensive with limited build size. Oak Ridge National Laboratory (ORNL) has developed a large scale AM system that improves upon each of these areas by more than an order of magnitude. The Big Area Additive Manufacturing (BAAM) system directly converts low cost pellets into a large, three-dimensional part at a rate exceeding 25 kg/h. By breaking these traditional barriers, it is possible for polymer AM to penetrate new manufacturing markets.

  4. Venezuela to double Supermetanol

    SciTech Connect (OSTI)

    1997-04-23

    Pequiven, the petrochemical arm of Venezuelan state oil company PDVSA, is conducting feasibility studies to double the size of its 750,000-m.t./year Supermetanol methanol joint venture with Ecofuel at Jose. The twin unit would be onstream by the end of the decade and would increase Pequiven`s capacity to 2.3 million m.t./year.

  5. Double resonator cantilever accelerometer

    DOE Patents [OSTI]

    Koehler, D.R.

    1982-09-23

    A digital quartz accelerometer includes a pair of spaced double-ended tuning forks fastened at one end to a base and at the other end through a spacer mass. Transverse movement of the resonator members stresses one and compresses the other, providing a differential frequency output which is indicative of acceleration.

  6. Double resonator cantilever accelerometer

    DOE Patents [OSTI]

    Koehler, Dale R.

    1984-01-01

    A digital quartz accelerometer includes a pair of spaced double-ended tuning forks fastened at one end to a base and at the other end through a spacer mass. Transverse movement of the resonator members stresses one and compresses the other, providing a differential frequency output which is indicative of acceleration.

  7. Chip breaking system for automated machine tool

    DOE Patents [OSTI]

    Arehart, Theodore A.; Carey, Donald O.

    1987-01-01

    The invention is a rotary selectively directional valve assembly for use in an automated turret lathe for directing a stream of high pressure liquid machining coolant to the interface of a machine tool and workpiece for breaking up ribbon-shaped chips during the formation thereof so as to inhibit scratching or other marring of the machined surfaces by these ribbon-shaped chips. The valve assembly is provided by a manifold arrangement having a plurality of circumferentially spaced apart ports each coupled to a machine tool. The manifold is rotatable with the turret when the turret is positioned for alignment of a machine tool in a machining relationship with the workpiece. The manifold is connected to a non-rotational header having a single passageway therethrough which conveys the high pressure coolant to only the port in the manifold which is in registry with the tool disposed in a working relationship with the workpiece. To position the machine tools the turret is rotated and one of the tools is placed in a material-removing relationship of the workpiece. The passageway in the header and one of the ports in the manifold arrangement are then automatically aligned to supply the machining coolant to the machine tool workpiece interface for breaking up of the chips as well as cooling the tool and workpiece during the machining operation.

  8. Analysis of Strand-Specific RNA-Seq Data Using Machine Learning...

    Office of Scientific and Technical Information (OSTI)

    Analysis of Strand-Specific RNA-Seq Data Using Machine Learning Reveals the Structures of Transcription Units in Clostridium thermocellum Citation Details In-Document Search Title: ...

  9. Double Beta Decay Experiments

    SciTech Connect (OSTI)

    Nanal, Vandana [Dept. of Nuclear and Atomic Physics, Tata Institute of Fundamental Research, Mumbai 400 005 (India)

    2011-11-23

    At present, neutrinoless double beta decay is perhaps the only experiment that can tell us whether the neutrino is a Dirac or a Majorana particle. Given the significance of the 0{nu}{beta}{beta}, there is a widespread interest for these rare event studies employing a variety of novel techniques. This paper describes the current status of DBD experiments. The Indian effort for an underground NDBD experiment at the upcoming INO laboratory is also presented.

  10. Breaking Ground in Miami-Dade

    Broader source: Energy.gov [DOE]

    Officials from Miami-Dade County and the U.S. Department of Energy were on hand Wednesday, October 13th to formally break ground on an innovative project that will help improve the energy efficiency of one of the county’s major water treatment facilities.   The project will upgrade and expand the existing power generation system at the water plant which generates electricity from digester gas produced at the plant.  Landfill gas, which is produced from the Solid Waste Department’s South Dade Landfill, will be collected and piped across a canal to the water plant where it will be mixed with digester gases.  By combining landfill and digester gases, the county will increase the amount of self-generated electricity, and reduce the county's consumption of electricity generated from fossil fuels.  

  11. Supersymmetry Breaking, Gauge Mediation, and the LHC

    SciTech Connect (OSTI)

    Shih, David

    2015-04-14

    Gauge mediated SUSY breaking (GMSB) is a promising class of supersymmetric models that automatically satisfies the precision constraints. Prior work of Meade, Seiberg and Shih in 2008 established the full, model-independent parameter space of GMSB, which they called "General Gauge Mediation" (GGM). During the first half of 2010-2015, Shih and his collaborators thoroughly explored the parameter space of GGM and established many well-motivated benchmark models for use by the experimentalists at the LHC. Through their work, the current constraints on GGM from LEP, the Tevatron and the LHC were fully elucidated, together with the possible collider signatures of GMSB at the LHC. This ensured that the full discovery potential for GGM could be completely realized at the LHC.

  12. A Gravity Dual of Metastable Dynamical Supersymmetry Breaking...

    Office of Scientific and Technical Information (OSTI)

    Subject: 72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS; DUALITY; EXPECTATION VALUE; RENORMALIZATION; SUPERGRAVITY; SUPERSYMMETRY; SYMMETRY BREAKING; SU GROUPS Theory-HEP,HEPTH...

  13. LANL breaks ground on key sediment control project

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Sediment control project LANL breaks ground on key sediment control project Called "grade-control" structures, the approximately 2 million features are up to eight feet high and...

  14. Pantex breaks ground on renewable energy project | National Nuclear...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    breaks ground on renewable energy project | National Nuclear Security Administration Facebook Twitter Youtube Flickr RSS People Mission Managing the Stockpile Preventing...

  15. Symmetry Breaking of H2 Dissociation by a Single Photon

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    breaking the molecular symmetry. A Molecular Paradox Symmetries in nature, such as the human body's bilateral symmetry and the snowflake's six-fold rotational symmetry, abound but...

  16. Breaking of Symmetry in Graphene Growth on Metal Substrates ...

    Office of Scientific and Technical Information (OSTI)

    Breaking of Symmetry in Graphene Growth on Metal Substrates Not Available Temp HTML Storage 2: Artyukhov, Vasilii I.; Hao, Yufeng; Ruoff, Rodney S.; Yakobson, Boris I. Temp Plain ...

  17. Breaking the Biological Barriers to Cellulosic Ethanol, June...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... including optical, X ray, elec- tron, and atomic force; and archives for molecular dynamic simulations of the molecular machinery that breaks down plant cell-wall components. ...

  18. NREL: Solar Research - New Partnerships Help Utilities Break...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    New Partnerships Help Utilities Break Down Solar Barriers May 11, 2016 The Solar Technical Assistance Team (STAT) Network launched its first program of technical assistance to ...

  19. Flavor symmetry breaking and vacuum alignment on orbifolds (Journal...

    Office of Scientific and Technical Information (OSTI)

    widely studied for figuring out the masses and mixing angles of standard model fermions. ... MIXING; POTENTIALS; SCALAR FIELDS; STANDARD MODEL; SYMMETRY; SYMMETRY BREAKING; ...

  20. ESnet's Science DMZ Breaks Down Barriers, Speeds up Science

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ESnet's Science DMZ Breaks Down Barriers, Speeds up Science News & Publications ESnet News Media & Press Publications and Presentations Galleries ESnet Awards and Honors Contact Us...

  1. Inter-strand current sharing and ac loss measurements in superconducting YBCO Roebel cables

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Majoros, M.; Sumption, M. D.; Collings, E. W.; Long, N. J.

    2015-04-08

    A Roebel cable, one twist pitch long, was modified from its as-received state by soldering copper strips between the strands to provide inter-strand connections enabling current sharing. Various DC transport currents (representing different percentages of its critical current) were applied to a single strand of such a modified cable at 77 K in a liquid nitrogen bath. Simultaneous monitoring of I–V curves in different parts of the strand as well as in its interconnections with other strands was made using a number of sensitive Keithley nanovoltmeters in combination with a multichannel high-speed data acquisition card, all controlled via LabView software.more » Current sharing onset was observed at about 1.02 of strand Ic. At a strand current of 1.3Ic about 5% of the current was shared through the copper strip interconnections. A finite element method modeling was performed to estimate the inter-strand resistivities required to enable different levels of current sharing. The relative contributions of coupling and hysteretic magnetization (and loss) were compared, and for our cable and tape geometry, and at dB/dt=1 T s-1, and our inter-strand resistance of 0.77 mΩ, (enabling a current sharing of 5% at 1.3Ic) the coupling component was 0.32% of the hysteretic component. However, inter-strand contact resistance values of 100–1000 times smaller (close to those of NbTi and Nb3Sn based accelerator cables) would make the coupling components comparable in size to the hysteretic components.« less

  2. Inter-strand current sharing and ac loss measurements in superconducting YBCO Roebel cables

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    sumption, Mike; Majoros, Milan; Collings, E. W.; Van der Laan, D. C.

    2014-11-07

    A Roebel cable, one twist pitch long, was modified from its as-received state by soldering copper strips between the strands to provide inter-strand connections enabling current sharing. Various DC transport currents (representing different percentages of its critical current) were applied to a single strand of such a modified cable at 77 K in a liquid nitrogen bath. Simultaneous monitoring of I–V curves in different parts of the strand as well as in its interconnections with other strands was made using a number of sensitive Keithley nanovoltmeters in combination with a multichannel high-speed data acquisition card, all controlled via LabView software.more » Current sharing onset was observed at about 1.02 of strand Ic. At a strand current of 1.3Ic about 5% of the current was shared through the copper strip interconnections. A finite element method modeling was performed to estimate the inter-strand resistivities required to enable different levels of current sharing. The relative contributions of coupling and hysteretic magnetization (and loss) were compared, and for our cable and tape geometry, and at dB/dt=1 T s-1, and our inter-strand resistance of 0.77 mΩ, (enabling a current sharing of 5% at 1.3Ic ) the coupling component was 0.32% of the hysteretic component. However, inter-strand contact resistance values of 100–1000 times smaller (close to those of NbTi and Nb3Sn based accelerator cables) would make the coupling components comparable in size to the hysteretic components.« less

  3. Inter-strand current sharing and ac loss measurements in superconducting YBCO Roebel cables

    SciTech Connect (OSTI)

    Majoros, M.; Sumption, M. D.; Collings, E. W.; Long, N. J.

    2015-04-08

    A Roebel cable, one twist pitch long, was modified from its as-received state by soldering copper strips between the strands to provide inter-strand connections enabling current sharing. Various DC transport currents (representing different percentages of its critical current) were applied to a single strand of such a modified cable at 77 K in a liquid nitrogen bath. Simultaneous monitoring of I–V curves in different parts of the strand as well as in its interconnections with other strands was made using a number of sensitive Keithley nanovoltmeters in combination with a multichannel high-speed data acquisition card, all controlled via LabView software. Current sharing onset was observed at about 1.02 of strand Ic. At a strand current of 1.3Ic about 5% of the current was shared through the copper strip interconnections. A finite element method modeling was performed to estimate the inter-strand resistivities required to enable different levels of current sharing. The relative contributions of coupling and hysteretic magnetization (and loss) were compared, and for our cable and tape geometry, and at dB/dt=1 T s-1, and our inter-strand resistance of 0.77 mΩ, (enabling a current sharing of 5% at 1.3Ic) the coupling component was 0.32% of the hysteretic component. However, inter-strand contact resistance values of 100–1000 times smaller (close to those of NbTi and Nb3Sn based accelerator cables) would make the coupling components comparable in size to the hysteretic components.

  4. Inter-strand current sharing and ac loss measurements in superconducting YBCO Roebel cables

    SciTech Connect (OSTI)

    sumption, Mike; Majoros, Milan; Collings, E. W.; Van der Laan, D. C.

    2014-11-07

    A Roebel cable, one twist pitch long, was modified from its as-received state by soldering copper strips between the strands to provide inter-strand connections enabling current sharing. Various DC transport currents (representing different percentages of its critical current) were applied to a single strand of such a modified cable at 77 K in a liquid nitrogen bath. Simultaneous monitoring of IV curves in different parts of the strand as well as in its interconnections with other strands was made using a number of sensitive Keithley nanovoltmeters in combination with a multichannel high-speed data acquisition card, all controlled via LabView software. Current sharing onset was observed at about 1.02 of strand Ic. At a strand current of 1.3Ic about 5% of the current was shared through the copper strip interconnections. A finite element method modeling was performed to estimate the inter-strand resistivities required to enable different levels of current sharing. The relative contributions of coupling and hysteretic magnetization (and loss) were compared, and for our cable and tape geometry, and at dB/dt=1 T s-1, and our inter-strand resistance of 0.77 m?, (enabling a current sharing of 5% at 1.3Ic ) the coupling component was 0.32% of the hysteretic component. However, inter-strand contact resistance values of 1001000 times smaller (close to those of NbTi and Nb3Sn based accelerator cables) would make the coupling components comparable in size to the hysteretic components.

  5. Characteristics of Cu stabilized Nb3Al strands with low Cu ratio

    SciTech Connect (OSTI)

    Kikuchi, A.; Yamada, R.; Barzi, E.; Kobayashi, M.; Lamm, M.; Nakagawa, K.; Sasaki, K.; Takeuchi, T.; Turrioni, D.; Zlobin, A.V.; /NIMC, Tsukuba /Fermilab /Hitachi, Tsuchiura Works /KEK, Tsukuba

    2008-12-01

    Characteristics of recently developed F4-Nb{sub 3}Al strand with low Cu ratio are described. The overall J{sub c} of the Nb{sub 3}Al strand could be easily increased by decreasing of the Cu ratio. Although the quench of a pulse-like voltage generation is usually observed in superconducting unstable conductor, the F4 strand with a low Cu ratio of 0.61 exhibited an ordinary critical transition of gradual voltage generation. The F4 strand does not have magnetic instabilities at 4.2 K because of the tantalum interfilament matrix. The overall J{sub c} of the F4 strand achieved was 80-85% of the RRP strand. In the large mechanical stress above 100 MPa, the overall J{sub c} of the F4 strand might be comparable to that of high J{sub c} RRP-Nb{sub 3}Sn strands. The Rutherford cable with a high packing factor of 86.5% has been fabricated using F4 strands. The small racetrack magnet, SR07, was also fabricated by a 14 m F4 cable. The quench current, I{sub q}, of SR07 were obtained 22.4 kA at 4.5 K and 25.2 kA at 2.2 K. The tantalum matrix Nb{sub 3}Al strands are promising for the application of super-cooled high-field magnets as well as 4.2 K operation magnets.

  6. Electrostatic supersolitons and double layers at the acoustic speed

    SciTech Connect (OSTI)

    Verheest, Frank; Hellberg, Manfred A.

    2015-01-15

    Supersolitons are characterized by subsidiary extrema on the sides of a typical bipolar electric field signature or by association with a root beyond double layers in the fully nonlinear Sagdeev pseudopotential description. It has been proven that supersolitons may exist in several plasmas having at least three constituent species, but they cannot be found in weakly nonlinear theory. Another recent aspect of pseudopotential theory is that in certain plasma models and parameter regimes solitons and/or double layers can exist at the acoustic speed, having no reductive perturbation counterparts. Importantly, they signal coexistence between solitons having positive and negative polarity, in that one solution can be realized at a time, depending on infinitesimal perturbations from the equilibrium state. Weaving the two strands together, we demonstrate here that one can even find supersolitons and double layers at the acoustic speed, as illustrated using the model of cold positive and negative ions, in the presence of nonthermal electrons following a Cairns distribution. This model has been discussed before, but the existence and properties of supersolitons at the acoustic speed were not established at the time of publication.

  7. Materials, Strands, and Cables for Superconducting Accelerator Magnets. Final Report

    SciTech Connect (OSTI)

    Sumption, Mike D.; Collings, Edward W.

    2014-09-19

    This report focuses on Materials, Strands and Cables for High Energy Physics Particle accelerators. In the materials area, work has included studies of basic reactions, diffusion, transformations, and phase assemblage of Nb3Sn. These materials science aspects have been married to results, in the form of flux pinning, Bc2, Birr, and transport Jc, with an emphasis on obtaining the needed Jc for HEP needs. Attention has also been paid to the “intermediate-temperature superconductor”, magnesium diboride emphasis being placed on (i) irreversibility field enhancement, (ii) critical current density and flux pinning, and (iii) connectivity. We also report on studies of Bi-2212. The second area of the program has been in the area of “Strands” in which, aside from the materials aspect of the conductor, its physical properties and their influence on performance have been studied. Much of this work has been in the area of magnetization estimation and flux jump calculation and control. One of the areas of this work was strand instabilities in high-performance Nb3Sn conductors due to combined fields and currents. Additionally, we investigated quench and thermal propagation in YBCO coated conductors at low temperatures and high fields. The last section, “Cables”, focussed on interstrand contact resistance, ICR, it origins, control, and implications. Following on from earlier work in NbTi, the present work in Nb3Sn has aimed to make ICR intermediate between the two extremes of too little contact (no current sharing) and too much (large and unacceptable magnetization and associated beam de-focussing). Interstrand contact and current sharing measurements are being made on YBCO based Roebel cables using transport current methods. Finally, quench was investigated for YBCO cables and the magnets wound from them, presently with a focus on 50 T solenoids for muon collider applications.

  8. Modulated Tool Path Chip Breaking Operation | Y-12 National Security

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Complex Modulated Tool Path Chip ... Modulated Tool Path Chip Breaking Operation The mp4 video format is not supported by this browser. Download video Captions: On Time: 2:09 min. The developers of the MTP chip-breaking system, an R&D 100 award winning technology, share some of the system's benefits and uses

  9. The hidden X-ray breaks in afterglow light curves

    SciTech Connect (OSTI)

    Curran, P. A.; Wijers, R. A. M. J.; Horst, A. J. van der; Starling, R. L. C.

    2008-05-22

    Gamma-Ray Burst (GRB) afterglow observations in the Swift era have a perceived lack of achromatic jet breaks compared to the BeppoSAX, or pre-Swift era. Specifically, relatively few breaks, consistent with jet breaks, are observed in the X-ray light curves of these bursts. If these breaks are truly missing, it has serious consequences for the interpretation of GRB jet collimation and energy requirements, and the use of GRBs as standard candles.Here we address the issue of X-ray breaks which are possibly 'hidden' and hence the light curves are misinterpreted as being single power-laws. We show how a number of precedents, including GRB 990510 and GRB 060206, exist for such hidden breaks and how, even with the well sampled light curves of the Swift era, these breaks may be left misidentified. We do so by synthesising X-ray light curves and finding general trends via Monte Carlo analysis. Furthermore, in light of these simulations, we discuss how to best identify achromatic breaks in afterglow light curves via multi-wavelength analysis.

  10. Leak before break evaluation for main steam piping system made of SA106 Gr.C

    SciTech Connect (OSTI)

    Yang, Kyoung Mo; Jee, Kye Kwang; Pyo, Chang Ryul; Ra, In Sik

    1997-04-01

    The basis of the leak before break (LBB) concept is to demonstrate that piping will leak significantly before a double ended guillotine break (DEGB) occurs. This is demonstrated by quantifying and evaluating the leak process and prescribing safe shutdown of the plant on the basis of the monitored leak rate. The application of LBB for power plant design has reduced plant cost while improving plant integrity. Several evaluations employing LBB analysis on system piping based on DEGB design have been completed. However, the application of LBB on main steam (MS) piping, which is LBB applicable piping, has not been performed due to several uncertainties associated with occurrence of steam hammer and dynamic strain aging (DSA). The objective of this paper is to demonstrate the applicability of the LBB design concept to main steam lines manufactured with SA106 Gr.C carbon steel. Based on the material properties, including fracture toughness and tensile properties obtained from the comprehensive material tests for base and weld metals, a parametric study was performed as described in this paper. The PICEP code was used to determine leak size crack (LSC) and the FLET code was used to perform the stability assessment of MS piping. The effects of material properties obtained from tests were evaluated to determine the LBB applicability for the MS piping. It can be shown from this parametric study that the MS piping has a high possibility of design using LBB analysis.

  11. Magnetization anomaly of Nb3Al strands and instability of Nb3Al Rutherford cables

    SciTech Connect (OSTI)

    Yamada, Ryuji; Kikuchi, Akihiro; Wake, Masayoshi; /KEK, Tsukuba

    2006-08-01

    Using a Cu stabilized Nb{sub 3}Al strand with Nb matrix, a 30 meter long Nb{sub 3}Al Rutherford cable was made by a collaboration of Fermilab and NIMS. Recently the strand and cable were tested. In both cases instability was observed at around 1.5 Tesla. The magnetization of this Nb{sub 3}Al strand was measured first using a balanced coil magnetometer at 4.2 K. Strands showed an anomalously large magnetization behavior around at 1.6 T, which is much higher than the usual B{sub c2} {approx} 0.5 Tesla (4.2 K) of Nb matrix. This result is compared with the magnetization data of short strand samples using a SQUID magnetometer, in which a flux-jump signal was observed at 0.5 Tesla, but not at higher field. As a possible explanation for this magnetization anomaly, the interfilament coupling through the thin Nb films in the strands is suggested. The instability problem observed in low field tests of the Nb{sub 3}Al Rutherford cables is attributed to this effect.

  12. A Gravity Dual of Metastable Dynamical Supersymmetry Breaking...

    Office of Scientific and Technical Information (OSTI)

    US0801780 DOE Contract Number: AC02-76SF00515 Resource ... Language: English Subject: 72 PHYSICS OF ELEMENTARY ... BREAKING; SU GROUPS Theory-HEP,HEPTH Word Cloud More ...

  13. Symmetry-Breaking Orbital Anisotropy Observed for Detwinned Ba...

    Office of Scientific and Technical Information (OSTI)

    Symmetry-Breaking Orbital Anisotropy Observed for Detwinned Ba(Fe (1-X) Co (X) ) (2) As (2) Above the Spin Density Wave Transition Citation Details In-Document Search Title: ...

  14. How Things Break (And Why Scientists Want to Know) | Argonne...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    This article was published in the spring 2016 issue of Argonne Now, the laboratory science magazine. Click for the rest of the issue. How Things Break (And Why Scientists Want to ...

  15. Double acting bit holder

    DOE Patents [OSTI]

    Morrell, Roger J.; Larson, David A.; Ruzzi, Peter L.

    1994-01-01

    A double acting bit holder that permits bits held in it to be resharpened during cutting action to increase energy efficiency by reducing the amount of small chips produced. The holder consist of: a stationary base portion capable of being fixed to a cutter head of an excavation machine and having an integral extension therefrom with a bore hole therethrough to accommodate a pin shaft; a movable portion coextensive with the base having a pin shaft integrally extending therefrom that is insertable in the bore hole of the base member to permit the moveable portion to rotate about the axis of the pin shaft; a recess in the movable portion of the holder to accommodate a shank of a bit; and a biased spring disposed in adjoining openings in the base and moveable portions of the holder to permit the moveable portion to pivot around the pin shaft during cutting action of a bit fixed in a turret to allow front, mid and back positions of the bit during cutting to lessen creation of small chip amounts and resharpen the bit during excavation use.

  16. Symmetry Breaking of H2 Dissociation by a Single Photon

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Symmetry Breaking of H2 Dissociation by a Single Photon Symmetry Breaking of H2 Dissociation by a Single Photon Print Wednesday, 25 July 2007 00:00 A single hydrogen (or deuterium) molecule consists of only two protons (deuterons) and two electrons and is perfectly symmetric. Linearly polarized photons are similarly symmetric. So one might think that the angular distribution of photoelectrons resulting from photoionization of the molecule by the photon accompanied by dissociation into a hydrogen

  17. Regional Test Centers Breaking Down Barriers to Solar Energy Deployment |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Regional Test Centers Breaking Down Barriers to Solar Energy Deployment Regional Test Centers Breaking Down Barriers to Solar Energy Deployment September 3, 2014 - 6:16pm Addthis EERE Assistant Secretary Dr. David Danielson stands with Senator Bernie Sanders of Vermont at the state's new regional solar test center. | Photo credit Kevin Fitzmaurice, Energy Department. EERE Assistant Secretary Dr. David Danielson stands with Senator Bernie Sanders of Vermont at the state's

  18. Energy Department Training Breaks New Ground | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Training Breaks New Ground Energy Department Training Breaks New Ground December 3, 2013 - 1:00pm Addthis The Federal Energy Management Program is now an authorized provider of continuing education units from the International Association for Continuing Education and Training. Watch the video above to learn more. Timothy Unruh Timothy Unruh FEMP Director High quality education for federal government employees helps streamline operations, improve services, and ensure that taxpayer dollars are

  19. Break-out Discussion i: Modeling Consumer Behavior Residential Scale |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Break-out Discussion i: Modeling Consumer Behavior Residential Scale Break-out Discussion i: Modeling Consumer Behavior Residential Scale This presentaion summarizes the information discussed during the breakout session on modeling consumer behavior during the DOE Ad Lucem Workshop on Feb. 17, 2012. adlucem2012_behavior_breakout.pdf (589.64 KB) More Documents & Publications Ad Lucem: Modeling of Market Transformation Pathways Workshop Agenda Modeling Solar Energy

  20. Deputy Secretary Poneman Attends Ground Breaking at Tennessee Advanced

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Vehicle Battery Plant | Department of Energy Attends Ground Breaking at Tennessee Advanced Vehicle Battery Plant Deputy Secretary Poneman Attends Ground Breaking at Tennessee Advanced Vehicle Battery Plant May 26, 2010 - 12:00am Addthis Smyrna, TN - Today, U.S. Deputy Secretary of Energy Daniel Poneman participated in the groundbreaking ceremony for Nissan North America's advanced battery manufacturing facility in Smyrna, Tennessee. This past January the Department closed a $1.4 billion loan

  1. EERE Success Story-Indiana Manufacturing Institute Breaks Ground at

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Purdue University in support of Composites Manufacturing Research | Department of Energy Indiana Manufacturing Institute Breaks Ground at Purdue University in support of Composites Manufacturing Research EERE Success Story-Indiana Manufacturing Institute Breaks Ground at Purdue University in support of Composites Manufacturing Research July 14, 2015 - 1:16pm Addthis Left: Gary Bertoline, Dean of Purdue Polytechnic Institute; Kelly Visconti, Technology Manager for the U.S. Department of

  2. More than $3 million raised during record-breaking Employee Giving...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Record-breaking employee giving campaign Community Connections: Your link to news and ... More than 3 million raised during record-breaking Employee Giving Campaign Contributions ...

  3. The Honorable Deborah L. Wince-Smith Discusses Breaking the Glass...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The Honorable Deborah L. Wince-Smith Discusses Breaking the Glass Ceiling in Manufacturing The Honorable Deborah L. Wince-Smith Discusses Breaking the Glass Ceiling in ...

  4. Search for: All records | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    ... laser-accelerated protons on human lung cancer cells.more In-vitro A549 cells are ... of DNA double-strand breaks in human cancer cells Yogo, A. ; Nishikino, M. ; Mori, M. ...

  5. Application of break preclusion concept in German nuclear power plants

    SciTech Connect (OSTI)

    Roos, E.; Maier, V.; Nagel, G.

    1997-04-01

    The break preclusion concept is based on {open_quotes}KTA rules{close_quotes}, {open_quotes}RSK guidelines{close_quotes} and {open_quotes}Rahmenspeziflkation Basissicherheit{close_quotes}. These fundamental rules containing for example requirements on material, design, calculation, manufacturing and testing procedures are explained and the technical realisation is shown by means of examples. The proof of the quality of these piping systems can be executed by means of fracture mechanics calculations by showing that in every case the leakage monitoring system already detect cracks which are clearly smaller than the critical crack. Thus the leak before break behavior and the break preclusion concept is implicitly affirmed. In order to further diminish conservativities in the fracture mechanics procedures, specific research projects are executed which are explained in this contribution.

  6. Strong Electroweak Symmetry Breaking and Spin-0 Resonances

    SciTech Connect (OSTI)

    Evans, Jared; Luty, Markus A.

    2009-09-04

    We argue that theories of the strong electroweak symmetry breaking sector necessarily contain new spin 0 states at the TeV scale in the tt and tb/bt channels, even if the third generation quarks are not composite at the TeV scale. These states couple sufficiently strongly to third generation quarks to have significant production at LHC via gg->phi{sup 0} or gb->tphi{sup -}. The existence of narrow resonances in QCD suggests that the strong electroweak breaking sector contains narrow resonances that decay to tt or tb/bt, with potentially significant branching fractions to 3 or more longitudinal W and Z bosons. These may give new 'smoking gun' signals of strong electroweak symmetry breaking.

  7. SB Electronics Breaks Ground on New Factory | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    SB Electronics Breaks Ground on New Factory SB Electronics Breaks Ground on New Factory April 29, 2010 - 5:22pm Addthis U.S. Rep. Peter Welch (from left), Vermont Lt. Gov. Brian Dubie, SBE board member Win Hunter, SBE board chair Stan Fishkin, Assi U.S. Rep. Peter Welch (from left), Vermont Lt. Gov. Brian Dubie, SBE board member Win Hunter, SBE board chair Stan Fishkin, Assi Paul Lester Paul Lester Digital Content Specialist, Office of Public Affairs A Vermont company broke ground on a new

  8. Margins in high temperature leak-before-break assessments

    SciTech Connect (OSTI)

    Budden, P.J.; Hooton, D.G.

    1997-04-01

    Developments in the defect assessment procedure R6 to include high-temperature mechanisms in Leak-before-Break arguments are described. In particular, the effect of creep on the time available to detect a leak and on the crack opening area, and hence leak rate, is discussed. The competing influence of these two effects is emphasized by an example. The application to Leak-before-Break of the time-dependent failure assessment diagram approach for high temperature defect assessment is then outlined. The approach is shown to be of use in assessing the erosion of margins by creep.

  9. Jefferson Lab Breaks Ground On $310 Million Project | Jefferson Lab

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Breaks Ground On $310 Million Project Jefferson Lab Breaks Ground On $310 Million Project groundbreaking Newport News Mayor Joe Frank addresses the crowd at the groundbreaking ceremony for the Jefferson Lab 12 GeV Upgrade. Also on the stage are (front row, l-r) U.S. Rep. Rob Wittman of Virginia's 1st District; U.S. Rep. Bobby Scott of Virginia's 3rd District; Gene Henry, Associate Director of the DOE's Office of Science for Nuclear Physics; Jefferson Lab Director Hugh Montgomery; (back row, l-r)

  10. Manganese oxide helices, rings, strands, and films, and methods for their preparation

    DOE Patents [OSTI]

    Suib, Steven L.; Giraldo, Oscar; Marquez, Manuel; Brock, Stephanie

    2003-01-07

    Methods for the preparation of mixed-valence manganese oxide compositions with quaternary ammonium ions are described. The compositions self-assemble into helices, rings, and strands without any imposed concentration gradient. These helices, rings, and strands, as well as films having the same composition, undergo rapid ion exchange to replace the quaternary ammonium ions with various metal ions. And the metal-ion-containing manganese oxide compositions so formed can be heat treated to form semi-conducting materials with high surface areas.

  11. Double field theory inspired cosmology

    SciTech Connect (OSTI)

    Wu, Houwen; Yang, Haitang E-mail: hyanga@scu.edu.cn

    2014-07-01

    Double field theory proposes a generalized spacetime action possessing manifest T-duality on the level of component fields. We calculate the cosmological solutions of double field theory with vanishing Kalb-Ramond field. It turns out that double field theory provides a more consistent way to construct cosmological solutions than the standard string cosmology. We construct solutions for vanishing and non-vanishing symmetry preserving dilaton potentials. The solutions assemble the pre- and post-big bang evolutions in one single line element. Our results show a smooth evolution from an anisotropic early stage to an isotropic phase without any special initial conditions in contrast to previous models. In addition, we demonstrate that the contraction of the dual space automatically leads to both an inflation phase and a decelerated expansion of the ordinary space during different evolution stages.

  12. Attosecond Double-Slit Experiment

    SciTech Connect (OSTI)

    Lindner, F.; Schaetzel, M.G.; Baltuska, A.; Goulielmakis, E.; Walther, H.; Krausz, F.; Milosevic, D.B.; Bauer, D.; Becker, W.; Paulus, G.G.

    2005-07-22

    A new scheme for a double-slit experiment in the time domain is presented. Phase-stabilized few-cycle laser pulses open one to two windows (slits) of attosecond duration for photoionization. Fringes in the angle-resolved energy spectrum of varying visibility depending on the degree of which-way information are measured. A situation in which one and the same electron encounters a single and a double slit at the same time is observed. The investigation of the fringes makes possible interferometry on the attosecond time scale. From the number of visible fringes, for example, one derives that the slits are extended over about 500 as.

  13. Towards breaking temperature equilibrium in multi-component Eulerian schemes

    SciTech Connect (OSTI)

    Grove, John W; Masser, Thomas

    2009-01-01

    We investigate the effects ofthermal equilibrium on hydrodynamic flows and describe models for breaking the assumption ofa single temperature for a mixture of components in a cell. A computational study comparing pressure-temperature equilibrium simulations of two dimensional implosions with explicit front tracking is described as well as implementation and J-D calculations for non-equilibrium temperature methods.

  14. How does a thermal binary crystal break under shear?

    SciTech Connect (OSTI)

    Horn, Tobias Löwen, Hartmut

    2014-12-14

    When exposed to strong shearing, the particles in a crystal will rearrange and ultimately, the crystal will break by forming large nonaffine defects. Even for the initial stage of this process, only little effort has been devoted to the understanding of the breaking process on the scale of the individual particle size for thermalized mixed crystals. Here, we explore the shear-induced breaking for an equimolar two-dimensional binary model crystal with a high interaction asymmetry between the two different species such that the initial crystal has an intersecting square sublattice of the two constituents. Using Brownian dynamics computer simulations, we show that the combination of shear and thermal fluctuations leads to a characteristic hierarchical breaking scenario where initially, the more strongly coupled particles are thermally distorted, paving the way for the weakly coupled particles to escape from their cage. This in turn leads to mobile defects which may finally merge, proliferating a cascade of defects, which triggers the final breakage of the crystal. This scenario is in marked contrast to the breakage of one-component crystals close to melting. Moreover, we explore the orientational dependence of the initial shear direction relative to the crystal orientation and compare this to the usual melting scenario without shear. Our results are verifiable in real-space experiments of superparamagnetic colloidal mixtures at a pending air-water interface in an external magnetic field where the shear can be induced by an external laser field.

  15. EECBG Success Story: Breaking Ground in Miami-Dade

    Broader source: Energy.gov [DOE]

    Officials from Miami-Dade County and the U.S. Department of Energy were on hand Wednesday, October 13th to formally break ground on an innovative project that will help improve the energy efficiency of one of the county’s major water treatment facilities. Learn more.

  16. Gedanken Worlds without Higgs: QCD-Induced Electroweak Symmetry Breaking

    SciTech Connect (OSTI)

    Quigg, Chris; Shrock, Robert; /YITP, Stony Brook

    2009-01-01

    To illuminate how electroweak symmetry breaking shapes the physical world, we investigate toy models in which no Higgs fields or other constructs are introduced to induce spontaneous symmetry breaking. Two models incorporate the standard SU(3){sub c} {circle_times} SU(2){sub L} {circle_times} U(1){sub Y} gauge symmetry and fermion content similar to that of the standard model. The first class--like the standard electroweak theory--contains no bare mass terms, so the spontaneous breaking of chiral symmetry within quantum chromodynamics is the only source of electroweak symmetry breaking. The second class adds bare fermion masses sufficiently small that QCD remains the dominant source of electroweak symmetry breaking and the model can serve as a well-behaved low-energy effective field theory to energies somewhat above the hadronic scale. A third class of models is based on the left-right-symmetric SU(3){sub c} {circle_times} SU(2){sub L} {circle_times} SU(2){sub R} {circle_times} U(1)B?L gauge group. In a fourth class of models, built on SU(4){sub PS} {circle_times} SU(2){sub L} {circle_times} SU(2){sub R} gauge symmetry, lepton number is treated as a fourth color. Many interesting characteristics of the models stem from the fact that the effective strength of the weak interactions is much closer to that of the residual strong interactions than in the real world. The Higgs-free models not only provide informative contrasts to the real world, but also lead us to consider intriguing issues in the application of field theory to the real world.

  17. Nb3Sn RRP® strand and Rutherford cable development for a 15 T dipole demonstrator

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Barzi, E.; Andreev, N.; Li, P.; Lombardo, V.; Turrioni, D.; Zlobin, A. V.

    2016-03-16

    Keystoned Rutherford cables made of 28 strands and with a stainless steel core were developed and manufactured using 1 mm Nb3Sn composite wires produced by Oxford Superconducting Technology with 127 and 169 restacks using the Restacked-Rod-Process®. Furthermore, the performance and properties of these cables were studied to evaluate possible candidates for 15 T accelerator magnets.

  18. The double well mass filter

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Gueroult, Renaud; Rax, Jean -Marcel; Fisch, Nathaniel J.

    2014-02-03

    Various mass filter concepts based on rotating plasmas have been suggested with the specific purpose of nuclear waste remediation. We report on a new rotating mass filter combining radial separation with axial extraction. Lastly, the radial separation of the masses is the result of a “double-well” in effective radial potential in rotating plasma with a sheared rotation profile.

  19. Traveling solitons in Lorentz and CPT breaking systems

    SciTech Connect (OSTI)

    Souza Dutra, A. de; Correa, R. A. C.

    2011-05-15

    In this work we present a class of traveling solitons in Lorentz and CPT breaking systems. In the case of Lorentz violating scenarios, as far as we know, only static solitonic configurations were analyzed up to now in the literature. Here it is shown that it is possible to construct some traveling solitons which cannot be mapped into static configurations by means of Lorentz boosts due to explicit breaking. In fact, the traveling solutions cannot be reached from the static ones by using something similar to a Lorentz boost in those cases. Furthermore, in the model studied, a complete set of exact solutions is obtained. The solutions present a critical behavior controlled by the choice of an arbitrary integration constant.

  20. Breaking discrete symmetries in the effective field theory of inflation

    SciTech Connect (OSTI)

    Cannone, Dario; Gong, Jinn-Ouk; Tasinato, Gianmassimo

    2015-08-03

    We study the phenomenon of discrete symmetry breaking during the inflationary epoch, using a model-independent approach based on the effective field theory of inflation. We work in a context where both time reparameterization symmetry and spatial diffeomorphism invariance can be broken during inflation. We determine the leading derivative operators in the quadratic action for fluctuations that break parity and time-reversal. Within suitable approximations, we study their consequences for the dynamics of linearized fluctuations. Both in the scalar and tensor sectors, we show that such operators can lead to new direction-dependent phases for the modes involved. They do not affect the power spectra, but can have consequences for higher correlation functions. Moreover, a small quadrupole contribution to the sound speed can be generated.

  1. Single-Sector Supersymmetry Breaking, Chirality, and Unification

    SciTech Connect (OSTI)

    Behbahani, Siavosh R.; Craig, Nathaniel; Torroba, Gonzalo; /SLAC /Stanford U., Phys. Dept.

    2011-08-12

    Calculable single-sector models provide an elegant framework for generating the flavor textures via compositeness, breaking supersymmetry, and explaining the electroweak scale. Such models may be realized naturally in supersymmetric QCD with additional gauge singlets (SSQCD), though it remains challenging to construct models without a surfeit of light exotic states where the Standard Model index emerges naturally. We classify possible single-sector models based on Sp confining SSQCD according to their Standard Model index and number of composite messengers. This leads to simple, calculable models that spontaneously break supersymmetry, reproduce the fermion flavor hierarchy, and explain the Standard Model index dynamically with little or no additional matter. At low energies these theories realize a 'more minimal' soft spectrum with direct mediation and a gravitino LSP.

  2. New Partnerships Help Utilities Break Down Solar Barriers | Energy Systems

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Integration | NREL New Partnerships Help Utilities Break Down Solar Barriers May 11, 2016 The Solar Technical Assistance Team (STAT) Network launched its first program of technical assistance to electric utilities and announced three new efforts to provide direct support to utility partners. The activities range from providing interconnection training to conducting detailed techno-economic modeling to leveraging community solar finance tools. The National Renewable Energy Laboratory (NREL),

  3. Symmetry Breaking of H2 Dissociation by a Single Photon

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Symmetry Breaking of H2 Dissociation by a Single Photon Print A single hydrogen (or deuterium) molecule consists of only two protons (deuterons) and two electrons and is perfectly symmetric. Linearly polarized photons are similarly symmetric. So one might think that the angular distribution of photoelectrons resulting from photoionization of the molecule by the photon accompanied by dissociation into a hydrogen atom and a hydrogen ion would itself be symmetric. However, an international team of

  4. Symmetry Breaking of H2 Dissociation by a Single Photon

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Symmetry Breaking of H2 Dissociation by a Single Photon Print A single hydrogen (or deuterium) molecule consists of only two protons (deuterons) and two electrons and is perfectly symmetric. Linearly polarized photons are similarly symmetric. So one might think that the angular distribution of photoelectrons resulting from photoionization of the molecule by the photon accompanied by dissociation into a hydrogen atom and a hydrogen ion would itself be symmetric. However, an international team of

  5. Symmetry Breaking of H2 Dissociation by a Single Photon

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Symmetry Breaking of H2 Dissociation by a Single Photon Print A single hydrogen (or deuterium) molecule consists of only two protons (deuterons) and two electrons and is perfectly symmetric. Linearly polarized photons are similarly symmetric. So one might think that the angular distribution of photoelectrons resulting from photoionization of the molecule by the photon accompanied by dissociation into a hydrogen atom and a hydrogen ion would itself be symmetric. However, an international team of

  6. Break-out Discussion i: Modeling Consumer Behavior Residential Scale

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Break-out Discussion I: Modeling Consumer Behavior Residential Scale  Are we asking the right questions?  What is a question we could ask about modeling consumer behavior that might lead to deeper insights into how to spur innovation? Why Are We Here? 2 Opower: visual comparison 3 opower 4 EERE Home Energy Score 5 * Similar comparison model * Adds climate context * Simple, visual  Data: - How much - What type  Data: - What is actionable today? - What is bodacious, over-the-top

  7. Symmetry-breaking instability of quadratic soliton bound states

    SciTech Connect (OSTI)

    Delque, Michaeel; Fanjoux, Gil; Maillotte, Herve; Kockaert, Pascal; Sylvestre, Thibaut; Haelterman, Marc

    2011-01-15

    We study both numerically and experimentally two-dimensional soliton bound states in quadratic media and demonstrate their symmetry-breaking instability. The experiment is performed in a potassium titanyl phosphate crystal in a type-II configuration. The bound state is generated by the copropagation of the antisymmetric fundamental beam locked in phase with the symmetrical second harmonic one. Experimental results are in good agreement with numerical simulations of the nonlinear wave equations.

  8. Sandia California breaks ground on new building | National Nuclear Security

    National Nuclear Security Administration (NNSA)

    Administration | (NNSA) breaks ground on new building Monday, August 3, 2015 - 11:11am A groundbreaking ceremony was recently held in Livermore, Calif., for a building that will enable consolidation of "front door" activities for Sandia National Laboratories California. NNSA Blog Building 926 will house the site's human resources department and will be home of the training center for students and new hires. The new 20,000 square foot facility, funded by institutional investments,

  9. Women in STEM careers: Breaking down barriers | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    This article was published in the spring 2016 issue of Argonne Now, the laboratory science magazine. Click for the rest of the issue. Women in STEM careers: Breaking down barriers By Diana Anderson * March 7, 2016 Tweet EmailPrint This article was originally published in the spring 2016 issue of Argonne Now, the laboratory's science magazine. Growing national attention is focused on increasing the number of women in Science, Technology, Engineering, and Mathematics (STEM) careers. Even as

  10. Symmetry Breaking of H2 Dissociation by a Single Photon

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Symmetry Breaking of H2 Dissociation by a Single Photon Print A single hydrogen (or deuterium) molecule consists of only two protons (deuterons) and two electrons and is perfectly symmetric. Linearly polarized photons are similarly symmetric. So one might think that the angular distribution of photoelectrons resulting from photoionization of the molecule by the photon accompanied by dissociation into a hydrogen atom and a hydrogen ion would itself be symmetric. However, an international team of

  11. LANL breaks ground on key sediment control project

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Sediment control project LANL breaks ground on key sediment control project Called "grade-control" structures, the approximately $2 million features are up to eight feet high and made of rocks packed tightly into wire enclosures. November 5, 2009 Los Alamos National Laboratory sits on top of a once-remote mesa in northern New Mexico with the Jemez mountains as a backdrop to research and innovation covering multi-disciplines from bioscience, sustainable energy sources, to plasma physics

  12. Soft supersymmetry breaking terms from D{sub 4}xZ{sub 2} lepton...

    Office of Scientific and Technical Information (OSTI)

    Soft supersymmetry breaking terms from Dsub 4xZsub 2 lepton flavor symmetry Citation Details In-Document Search Title: Soft supersymmetry breaking terms from Dsub 4xZsub 2 ...

  13. U.S. Department of Energy Breaks Ground on State-of-the-Art Wind...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Breaks Ground on State-of-the-Art Wind Turbine Test Facility U.S. Department of Energy Breaks Ground on State-of-the-Art Wind Turbine Test Facility October 1, 2012 - 12:08pm ...

  14. Green Future Double Barrel House

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    University Green Future Double Barrel House DOE Race to Zero Student Competition 2016 Sean Benson Team Leader - Net Zero Energy Design I & II Bachelor of Science in Architecture, Dec 2016 Alexis Borman Net Zero Energy Design II Bachelor of Science in Architecture, May 2016 Christopher Brown AIA COTE, Net Zero Energy Design I & II Bachelor of Science in Architecture, May 2016 Devonta Magee Net Zero Energy Design II Bachelor of Science in Architecture, Aug 2016 Yasmine Parker Net Zero

  15. DNA purification by triplex-affinity capture and affinity capture electrophoresis

    DOE Patents [OSTI]

    Cantor, Charles R.; Ito, Takashi; Smith, Cassandra L.

    1996-01-01

    The invention provides a method for purifying or isolating double stranded DNA intact using triple helix formation. The method includes the steps of complexing an oligonucleotide and double stranded DNA to generate a triple helix and immobilization of the triple helix on a solid phase by means of a molecular recognition system such as avidin/biotin. The purified DNA is then recovered intact by treating the solid phase with a reagent that breaks the bonds between the oligonucleotide and the intact double stranded DNA while not affecting the Watson-Crick base pairs of the double helix. The present invention also provides a method for purifying or isolating double stranded DNA intact by complexing the double stranded DNA with a specific binding partner and recovering the complex during electrophoresis by immobilizing it on a solid phase trap imbedded in an electrophoretic gel.

  16. DNA purification by triplex-affinity capture and affinity capture electrophoresis

    DOE Patents [OSTI]

    Cantor, C.R.; Ito, Takashi; Smith, C.L.

    1996-01-09

    The invention provides a method for purifying or isolating double stranded DNA intact using triple helix formation. The method includes the steps of complexing an oligonucleotide and double stranded DNA to generate a triple helix and immobilization of the triple helix on a solid phase by means of a molecular recognition system such as avidin/biotin. The purified DNA is then recovered intact by treating the solid phase with a reagent that breaks the bonds between the oligonucleotide and the intact double stranded DNA while not affecting the Watson-Crick base pairs of the double helix. The present invention also provides a method for purifying or isolating double stranded DNA intact by complexing the double stranded DNA with a specific binding partner and recovering the complex during electrophoresis by immobilizing it on a solid phase trap imbedded in an electrophoretic gel. 6 figs.

  17. Doubling Geothermal Generation Capacity by 2020: A Strategic...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Doubling Geothermal Generation Capacity by 2020: A Strategic Analysis Doubling Geothermal Generation Capacity by 2020: A Strategic Analysis PDF icon NREL Doubling Geothermal ...

  18. Experiment data report for LOFT large-break loss-of-coolant experiment L2-5. [PWR

    SciTech Connect (OSTI)

    Bayless, P.D.; Divine, J.M.

    1982-08-01

    Selected pertinent and uninterpreted data from the third nuclear large break loss-of-coolant experiment (Experiment L2-5) conducted in the Loss-of-Fluid Test (LOFT) facility are presented. The LOFT facility is a 50-MW(t) pressurized water reactor (PWR) system with instruments that measure and provide data on the system thermal-hydraulic and nuclear conditions. The operation of the LOFT system is typical of large (approx. 1000 MW(e)) commercial PWR operations. Experiment L2-5 simulated a double-ended offset shear of a cold leg in the primary coolant system. The primary coolant pumps were tripped within 1 s after the break initiation, simulating a loss of site power. Consistent with the loss of power, the starting of the high- and low-pressure injection systems was delayed. The peak fuel rod cladding temperature achieved was 1078 +- 13 K. The emergency core cooling system re-covered the core and quenched the cladding. No evidence of core damage was detected.

  19. Method for nucleic acid hybridization using single-stranded DNA binding protein

    DOE Patents [OSTI]

    Tabor, Stanley; Richardson, Charles C.

    1996-01-01

    Method of nucleic acid hybridization for detecting the presence of a specific nucleic acid sequence in a population of different nucleic acid sequences using a nucleic acid probe. The nucleic acid probe hybridizes with the specific nucleic acid sequence but not with other nucleic acid sequences in the population. The method includes contacting a sample (potentially including the nucleic acid sequence) with the nucleic acid probe under hybridizing conditions in the presence of a single-stranded DNA binding protein provided in an amount which stimulates renaturation of a dilute solution (i.e., one in which the t.sub.1/2 of renaturation is longer than 3 weeks) of single-stranded DNA greater than 500 fold (i.e., to a t.sub.1/2 less than 60 min, preferably less than 5 min, and most preferably about 1 min.) in the absence of nucleotide triphosphates.

  20. Evaluation of Juvenile Fall Chinook Stranding on the Hanford Reach, 1997-1999 Interim Report.

    SciTech Connect (OSTI)

    Wagner, Paul; Nugent, John; Price, William

    1999-02-15

    Pilot work conducted in 1997 to aid the development of the study for the 1998 Evaluation of Juvenile Fall Chinook Stranding on The Hanford Reach. The objectives of the 1997 work were to: (1) identify juvenile chinook production and rearing areas..., (2) identify sampling sites and develop the statistical parameters necessary to complete the study, (3) develop a study plan..., (4) conduct field sampling activities...

  1. Booster double harmonic setup notes

    SciTech Connect (OSTI)

    Gardner, C. J.

    2015-02-17

    The motivation behind implementing a booster double harmonic include the reduced transverse space charge force from a reduced peak beam current and reduced momentum spread of the beam, both of which can be achieved from flattening the RF bucket. RF capture and acceleration of polarized protons (PP) is first set up in the single harmonic mode with RF harmonic h=1. Once capture and acceleration have been set up in the single harmonic mode, the second harmonic system is brought on and programmed to operate in concert with the single harmonic system.

  2. Leak before break application in French PWR plants under operation

    SciTech Connect (OSTI)

    Faidy, C.

    1997-04-01

    Practical applications of the leak-before break concept are presently limited in French Pressurized Water Reactors (PWR) compared to Fast Breeder Reactors. Neithertheless, different fracture mechanic demonstrations have been done on different primary, auxiliary and secondary PWR piping systems based on similar requirements that the American NUREG 1061 specifications. The consequences of the success in different demonstrations are still in discussion to be included in the global safety assessment of the plants, such as the consequences on in-service inspections, leak detection systems, support optimization,.... A large research and development program, realized in different co-operative agreements, completes the general approach.

  3. Double distributions and evolution equations

    SciTech Connect (OSTI)

    A.V. Radyushkin

    1998-05-01

    Applications of perturbative QCD to deeply virtual Compton scattering and hard exclusive meson electroproduction processes require a generalization of usual parton distributions for the case when long-distance information is accumulated in nonforward matrix elements < p{prime} {vert_bar}O(0,z){vert_bar}p > of quark and gluon light-cone operators. In their previous papers the authors used two types of nonperturbative functions parameterizing such matrix elements: double distributions F(x,y;t) and nonforward distribution functions F{sub {zeta}}(X;t). Here they discuss in more detail the double distributions (DD's) and evolution equations which they satisfy. They propose simple models for F(x,y;t=0) DD's with correct spectral and symmetry properties which also satisfy the reduction relations connecting them to the usual parton densities f(x). In this way, they obtain self-consistent models for the {zeta}-dependence of nonforward distributions. They show that, for small {zeta}, one can easily obtain nonforward distributions (in the X > {zeta} region) from the parton densities: F{sub {zeta}} (X;t=0) {approx} f(X{minus}{zeta}/2).

  4. Effects of hot electron inertia on electron-acoustic solitons and double layers

    SciTech Connect (OSTI)

    Verheest, Frank; Hellberg, Manfred A.

    2015-07-15

    The propagation of arbitrary amplitude electron-acoustic solitons and double layers is investigated in a plasma containing cold positive ions, cool adiabatic and hot isothermal electrons, with the retention of full inertial effects for all species. For analytical tractability, the resulting Sagdeev pseudopotential is expressed in terms of the hot electron density, rather than the electrostatic potential. The existence domains for Mach numbers and hot electron densities clearly show that both rarefactive and compressive solitons can exist. Soliton limitations come from the cool electron sonic point, followed by the hot electron sonic point, until a range of rarefactive double layers occurs. Increasing the relative cool electron density further yields a switch to compressive double layers, which ends when the model assumptions break down. These qualitative results are but little influenced by variations in compositional parameters. A comparison with a Boltzmann distribution for the hot electrons shows that only the cool electron sonic point limit remains, giving higher maximum Mach numbers but similar densities, and a restricted range in relative hot electron density before the model assumptions are exceeded. The Boltzmann distribution can reproduce neither the double layer solutions nor the switch in rarefactive/compressive character or negative/positive polarity.

  5. Encapsulation of Protonated Diamines in a Water-Soluble Chiral, Supramolecular Assembly Allows for Measurement of Hydrogen-Bond Breaking Followed by Nitrogen Inversion/Rotation (NIR)

    SciTech Connect (OSTI)

    Meux, Susan C.; Pluth, Michael D.; Bergman, Robert G.; Raymond, Kenneth N.

    2007-09-19

    Amine nitrogen inversion, difficult to observe in aqueous solution, is followed in a chiral, supramolecular host molecule with purely-rotational T-symmetry that reduces the local symmetry of encapsulated monoprotonated diamines and enables the observation and quantification of {Delta}G{double_dagger} for the combined hydrogen-bond breaking and nitrogen inversion rotation (NIR) process. Free energies of activation for the combined hydrogen-bond breaking and NIR process inside of the chiral assembly were determined by the NMR coalescence method. Activation parameters for ejection of the protonated amines from the assembly confirm that the NIR process responsible for the coalescence behavior occurs inside of the assembly rather than by a guest ejection/NIR/re-encapsulation mechanism. For one of the diamines, N,N,N{prime},N{prime}-tetramethylethylenediamine (TMEDA), the relative energy barriers for the hydrogen-bond breaking and NIR process were calculated at the G3(MP2)//B3LYP/6-31++G(d,p) level of theory, and these agreed well with the experimental data.

  6. Flavour symmetry breaking in the kaon parton distribution amplitude

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    none,

    2014-11-01

    We compute the kaon's valence-quark (twist-two parton) distribution amplitude (PDA) by projecting its Poincar-covariant BetheSalpeter wave-function onto the light-front. At a scale ? = 2 GeV, the PDA is a broad, concave and asymmetric function, whose peak is shifted 1216% away from its position in QCD's conformal limit. These features are a clear expression of SU(3)-flavour-symmetry breaking. They show that the heavier quark in the kaon carries more of the bound-state's momentum than the lighter quark and also that emergent phenomena in QCD modulate the magnitude of flavour-symmetry breaking: it is markedly smaller than one might expect based on themoredifference between light-quark current masses. Our results add to a body of evidence which indicates that at any energy scale accessible with existing or foreseeable facilities, a reliable guide to the interpretation of experiment requires the use of such nonperturbatively broadened PDAs in leading-order, leading-twist formulae for hard exclusive processes instead of the asymptotic PDA associated with QCD's conformal limit. We illustrate this via the ratio of kaon and pion electromagnetic form factors: using our nonperturbative PDAs in the appropriate formulae, FK/F?=1.23 at spacelike-Q2=17 GeV2, which compares satisfactorily with the value of 0.92(5) inferred in e+e- annihilation at s=17 GeV2.less

  7. Intrinsic transverse momentum and dynamical chiral symmetry breaking

    SciTech Connect (OSTI)

    Christian Weiss, Peter Schweitzer, Mark Strikman

    2013-01-01

    We study the effect of QCD vacuum structure on the intrinsic transverse momentum distribution of partons in the nucleon at a low scale. The dynamical breaking of chiral symmetry is caused by non-perturbative interactions at distances of the order rho ~ 0.2 - 0.3 fm, much smaller than the typical nucleon size R ~ 1 fm, resulting in a two-scale picture of nucleon structure. Using an effective dynamical model based on chiral constituent quark degrees of freedom and the 1/N_c expansion (chiral quark-soliton model), we calculate the transverse momentum distribution of quarks and antiquarks at a low scale. The distribution of valence quarks is localized at p_T ~ 1/R. The distribution of flavor-singlet unpolarized sea quarks exhibits a power-like tail extending up to the chiral-symmetry-breaking scale 1/{rho}. A similar tail is present in the flavor-nonsinglet polarized sea. These features are model-independent and represent the imprint of the QCD vacuum on the nucleon's partonic structure. At the level of the nucleon's light-cone wave function, we show that sea quarks partly exist in correlated pairs of transverse size {rho} << R, analogous to short-range NN correlations in nuclei. We discuss the implications of our findings for the transverse momentum distributions in hard scattering processes (semi-inclusive DIS, Drell-Yan pair production) and possible experimental tests of the non-perturbative parton correlations induced by QCD vacuum structure.

  8. Double perovskite catalysts for oxidative coupling

    DOE Patents [OSTI]

    Campbell, Kenneth D.

    1991-01-01

    Alkali metal doped double perovskites containing manganese and at least one of cobalt, iron and nickel are useful in the oxidative coupling of alkane to higher hydrocarbons.

  9. Ligation of single-stranded oligodeoxyribonucleotides by T4 RNA ligase

    SciTech Connect (OSTI)

    Tessier, D.C.; Brousseau, R.; Vernet, T.

    1986-10-01

    Despite its unique ability to ligate single-stranded DNA molecules, T4 RNA ligase has so far seen little use in molecular biology due to long reaction times, modest yields, and apparent inability to promote ligations of long oligodeoxyribonucleotides. The authors describe here a set of reaction conditions which dramatically shorten the reaction time and give reproducible 40 to 60% ligation of DNA fragments of up to 40 bases in length. These improvements open promising new fields of application to T4 RNA ligase.

  10. Segregation of DNA polynucleotide strands into sister chromatids and the use of endoreduplicated cells to track sister chromatid exchanges induced by crosslinks, alkylations, or x-ray damage

    SciTech Connect (OSTI)

    Wolff, S.; Afzal, V.

    1996-06-11

    The method of Matsumoto and Ohta to induce large numbers of endoreduplicated Chinese hamster ovary cells has now been coupled with the fluorescence-plus-Giemsa method of Perry and Wolff to produce harlequin endoreduplicated chromosomes that after the third round of DNA replication are composed of a chromosome with a light chromatid and a dark chromatid in close apposition to its sister chromosome containing two light chromatids. Unless the pattern is disrupted by sister chromatid exchange (SCE), the dark chromatid is always in the center, so that the order of the chromatids is light-dark light-light. The advent of this method, which permits the observation of SCEs in endoreduplicated cells, makes it possible to determine with great ease in which cell cycle an SCE occurred. This now allows us to approach several vexing questions about the induction of SCEs (genetic damage and its repair) after exposure to various types of mutagenic carcinogens. The present experiments have allowed observation of how many cell cycles various types of lesions that are induced in DNA by a crosslinking agent, an alkylating agent, or ionizing radiation, and that are responsible for the induction of SCEs, persist before being repaired and thus lose their ability to inflict genetic damage. Other experiments with various types of mutagenic carcinogens and various types of cell lines that have defects in different DNA repair processes, such as mismatch repair, excision repair, crosslink repair, and DNA-strand-break repair, can now be carried out to determine the role of these types of repair in removing specific types of lesions. 22 refs., 3 figs., 1 tab.

  11. CSNI specialist meeting on leak-before-break in nuclear reactor piping: proceedings

    SciTech Connect (OSTI)

    Not Available

    1984-08-01

    On September 1 and 2, 1983, the CSNI subcommittee on primary system integrity held a special meeting in Monterey, California, on the subject of leak-before-break in nuclear reactor piping systems. The purpose of the meeting was to provide an international forum for the exchange of ideas, positions, and research results; to identify areas requiring additional research and development; and to determine the general attitude toward acceptance of the leak-before-break concept. The importance of the leak-before-break issue was evidenced by excellent attendance at the meeting and through active participation by the meeting attendees. Approximately 125 people representing fifteen different nations attended the meeting. The meeting was divided into four technical sessions addressing the following areas: Application of Piping Fracture Mechanics to Leak-Before Break, Leak Rate and Leak Detection, Leak-Before-Break Studies, Methods and Results, Current and Proposed Positions on Leak-Before-Break.

  12. Evaluation of a main steam line break with induced, multiple tube ruptures: A comparison of NUREG 1477 (Draft) and transient methodologies Palo Verde Nuclear Generating Station

    SciTech Connect (OSTI)

    Parrish, K.R.

    1995-09-01

    This paper presents the approach taken to analyze the radiological consequences of a postulated main steam line break event, with one or more tube ruptures, for the Palo Verde Nuclear Generating Station. The analysis was required to support the restart of PVNGS Unit 2 following the steam generator tube rupture event on March 14, 1993 and to justify continued operation of Units 1 and 3. During the post-event evaluation, the NRC expressed concern that Unit 2 could have been operating with degraded tubes and that similar conditions could exist in Units 1 and 3. The NRC therefore directed that a safety assessment be performed to evaluate a worst case scenario in which a non-isolable main steam line break occurs inducing one or more tube failures in the faulted steam generator. This assessment was to use the generic approach described in NUREG 1477, Voltage-Based Interim Plugging Criteria for Steam Generator Tubes - Task Group Report. An analysis based on the NUREG approach was performed but produced unacceptable results for off-site and control room thyroid doses. The NUREG methodology, however, does not account for plant thermal-hydraulic transient effects, system performance, or operator actions which could be credited to mitigate dose consequences. To deal with these issues, a more detailed analysis methodology was developed using a modified version of the Combustion Engineering Plant Analysis Code, which examines the dose consequences for a main steam line break transient with induced tube failures for a spectrum equivalent to 1 to 4 double ended guillotine U-tube breaks. By incorporating transient plant system responses and operator actions, the analysis demonstrates that the off-site and control room does consequences for a MSLBGTR can be reduced to acceptable limits. This analysis, in combination with other corrective and recovery actions, provided sufficient justification for continued operation of PVNGS Units 1 and 3, and for the subsequent restart of Unit 2.

  13. Spontaneous supersymmetry breaking in two dimensional lattice super QCD

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Catterall, Simon; Veernala, Aarti

    2015-10-02

    We report on a non-perturbative study of two dimensional N=(2,2) super QCD. Our lattice formulation retains a single exact supersymmetry at non-zero lattice spacing, and contains Nf fermions in the fundamental representation of a U(Nc) gauge group. The lattice action we employ contains an additional Fayet-Iliopoulos term which is also invariant under the exact lattice supersymmetry. This work constitutes the first numerical study of this theory which serves as a toy model for understanding some of the issues that are expected to arise in four dimensional super QCD. As a result, we present evidence that the exact supersymmetry breaks spontaneouslymore » when Nf < Nc in agreement with theoretical expectations.« less

  14. Spontaneous supersymmetry breaking in two dimensional lattice super QCD

    SciTech Connect (OSTI)

    Catterall, Simon; Veernala, Aarti

    2015-10-02

    We report on a non-perturbative study of two dimensional N=(2,2) super QCD. Our lattice formulation retains a single exact supersymmetry at non-zero lattice spacing, and contains Nf fermions in the fundamental representation of a U(Nc) gauge group. The lattice action we employ contains an additional Fayet-Iliopoulos term which is also invariant under the exact lattice supersymmetry. This work constitutes the first numerical study of this theory which serves as a toy model for understanding some of the issues that are expected to arise in four dimensional super QCD. As a result, we present evidence that the exact supersymmetry breaks spontaneously when Nf < Nc in agreement with theoretical expectations.

  15. Española entrepreneur breaks ground; expansion will create 50 new jobs

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Española entrepreneur breaks ground; expansion will create 50 new jobs Community Connections: Your link to news and opportunities from Los Alamos National Laboratory Latest Issue: September 1, 2016 all issues All Issues » submit Española entrepreneur breaks ground; expansion will create 50 new jobs Governor Susana Martinez initiated new site on June 17 August 1, 2014 Breaking ground at PMI's new site. Española Mayor Alice Lucero, New Mexico Economic Development Dept. Cabinet Secretary Jon

  16. ON THE EVOLUTION OF THE SPECTRAL BREAK IN THE AFTERGLOW OF GAMMA-RAY BURSTS

    SciTech Connect (OSTI)

    Dado, Shlomo; Dar, Arnon [Physics Department, Technion, Haifa 32000 (Israel)

    2012-02-20

    The temporal evolution of the spectral break in the time-resolved spectral energy density of the broadband afterglow of gamma-ray bursts (GRBs) 091127 and 080319B was shown recently to be inconsistent with that expected for the cooling break in the standard fireball model of GRBs. Here we show that it is, however, in good agreement with the predicted temporal evolution of the smooth injection break/bend in the cannonball model of GRBs.

  17. Double bevel construction of a diamond anvil

    DOE Patents [OSTI]

    Moss, William C.

    1988-01-01

    A double or multiple bevel culet geometry is used on a diamond anvil in a high pressure cell apparatus to provide increased sample pressure and stability for a given force applied to the diamond tables. Double or multiple bevel culet geometries can also be used for sapphire or other hard crystal anvils. Pressures up to and above 5 Megabars can be reached.

  18. Double bevel construction of a diamond anvil

    DOE Patents [OSTI]

    Moss, W.C.

    1988-10-11

    A double or multiple bevel culet geometry is used on a diamond anvil in a high pressure cell apparatus to provide increased sample pressure and stability for a given force applied to the diamond tables. Double or multiple bevel culet geometries can also be used for sapphire or other hard crystal anvils. Pressures up to and above 5 Megabars can be reached. 8 figs.

  19. Break-Even Cost for Residential Solar Water Heating in the United...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Break-even Cost for Residential Solar Water Heating in the United States: Key Drivers and Sensitivities Hannah Cassard, Paul Denholm, and Sean Ong Technical Report NREL...

  20. Final Report: MATERIALS, STRANDS, AND CABLES FOR SUPERCONDUCTING ACCELERATOR MAGNETS [Grant Number DE-SC0010312

    SciTech Connect (OSTI)

    Sumption, Mike; Collings, E.

    2014-10-29

    Our program consisted of the two components: Strand Research and Cable Research, with a focus on Nb3Sn, Bi2212, and YBCO for accelerator magnet applications. We demonstrated a method to refine the grains in Nb3Sn by a factor of two, reaching 45 nm grain sizes, and layer Jcs of 6 kA/mm2 at 12 T. W also measured conductor magnetization for field quality. This has been done both with Nb3Sn conductor, as well as Bi:2212 strand. Work in support of quench studies of YBCO coils was also performed. Cable loss studies in Nb3Sn focused on connecting and comparing persistent magnetization and coupling magnetization for considering their relative impact on HEP machines. In the area of HTS cables, we have investigated both the quench in multistrand YBCO CORC cables, as well as the magnetization of these cables for use in high field magnets. In addition, we examined the magnetic and thermal properties of large (50 T) solenoids.

  1. Reliability Estimation for Double Containment Piping

    SciTech Connect (OSTI)

    L. Cadwallader; T. Pinna

    2012-08-01

    Double walled or double containment piping is considered for use in the ITER international project and other next-generation fusion device designs to provide an extra barrier for tritium gas and other radioactive materials. The extra barrier improves confinement of these materials and enhances safety of the facility. This paper describes some of the design challenges in designing double containment piping systems. There is also a brief review of a few operating experiences of double walled piping used with hazardous chemicals in different industries. This paper recommends approaches for the reliability analyst to use to quantify leakage from a double containment piping system in conceptual and more advanced designs. The paper also cites quantitative data that can be used to support such reliability analyses.

  2. Instability limits for spontaneous double layer formation

    SciTech Connect (OSTI)

    Carr, J. Jr.; Department of Physics, Texas Lutheran University, Seguin, Texas 78155 ; Galante, M. E.; McCarren, D.; Scime, E. E.; Sears, S.; VanDervort, R. W.; Magee, R. M.; TriAlpha Energy, Inc., Foothill Ranch, California 92610 ; Reynolds, E.

    2013-11-15

    We present time-resolved measurements that demonstrate that large amplitude electrostatic instabilities appear in pulsed, expanding helicon plasmas at the same time as particularly strong double layers appear in the expansion region. A significant cross-correlation between the electrostatic fluctuations and fluctuations in the number of ions accelerated by the double layer electric field is observed. No correlation is observed between the electrostatic fluctuations and ions that have not passed through the double layer. These measurements confirm that the simultaneous appearance of the electrostatic fluctuations and the double layer is not simple coincidence. In fact, the accelerated ion population is responsible for the growth of the instability. The double layer strength, and therefore, the velocity of the accelerated ions, is limited by the appearance of the electrostatic instability.

  3. Double-disc gate valve

    DOE Patents [OSTI]

    Wheatley, Seth J.

    1979-01-01

    This invention relates to an improvement in a conventional double-disc gate valve having a vertically movable gate assembly including a wedge, spreaders slidably engaged therewtih, a valve disc carried by the spreaders. When the gate assembly is lowered to a selected point in the valve casing, the valve discs are moved transversely outward to close inlet and outlet ports in the casing. The valve includes hold-down means for guiding the disc-and-spreader assemblies as they are moved transversely outward and inward. If such valves are operated at relatively high differential pressures, they sometimes jam during opening. Such jamming has been a problem for many years in gate valves used in gaseous diffusion plants for the separtion of uranium isotopes. The invention is based on the finding that the above-mentioned jamming results when the outlet disc tilts about its horizontal axis in a certain way during opening of the valve. In accordance with the invention, tilting of the outlet disc is maintained at a tolerable value by providing the disc with a rigid downwardly extending member and by providing the casing with a stop for limiting inward arcuate movement of the member to a preselected value during opening of the valve.

  4. Belgian experience in applying the {open_quotes}leak-before-break{close_quotes} concept to the primary loop piping

    SciTech Connect (OSTI)

    Gerard, R.; Malekian, C.; Meessen, O.

    1997-04-01

    The Leak Before Break (LBB) concept allows to eliminate from the design basis the double-ended guillotine break of the primary loop piping, provided it can be demonstrated by a fracture mechanics analysis that a through-wall flaw, of a size giving rise to a leakage still well detectable by the plant leak detection systems, remains stable even under accident conditions (including the Safe Shutdown Earthquake (SSE)). This concept was successfully applied to the primary loop piping of several Belgian Pressurized Water Reactor (PWR) units, operated by the Utility Electrabel. One of the main benefits is to permit justification of supports in the primary loop and justification of the integrity of the reactor pressure vessel and internals in case of a Loss Of Coolant Accident (LOCA) in stretch-out conditions. For two of the Belgian PWR units, the LBB approach also made it possible to reduce the number of large hydraulic snubbers installed on the primary coolant pumps. Last but not least, the LBB concept also facilitates the steam generator replacement operations, by eliminating the need for some pipe whip restraints located close to the steam generator. In addition to the U.S. regulatory requirements, the Belgian safety authorities impose additional requirements which are described in details in a separate paper. An novel aspect of the studies performed in Belgium is the way in which residual loads in the primary loop are taken into account. Such loads may result from displacements imposed to close the primary loop in a steam generator replacement operation, especially when it is performed using the {open_quote}two cuts{close_quotes} technique. The influence of such residual loads on the LBB margins is discussed in details and typical results are presented.

  5. Development of double-decker pulse radiolysis

    SciTech Connect (OSTI)

    Kan, K.; Kondoh, T.; Yang, J.; Ogata, A.; Norizawa, K.; Yoshida, Y.

    2012-07-15

    Double-decker pulse radiolysis (DDPR), which utilizes double-decker electron beams, was investigated to develop a new pulse radiolysis with a high time resolution. The double-decker electron beams were generated by injecting two UV pulses into a photocathode radio-frequency gun. In the pulse radiolysis, one electron beam was used as a pump beam, and the other was converted to a probe pulse. Finally, as its first application, the DDPR was successfully used for observing solvated electrons in water, with a 10%-90% rise time of 8.6 ps.

  6. Double-reed exhaust valve engine

    DOE Patents [OSTI]

    Bennett, Charles L.

    2015-06-30

    An engine based on a reciprocating piston engine that extracts work from pressurized working fluid. The engine includes a double reed outlet valve for controlling the flow of low-pressure working fluid out of the engine. The double reed provides a stronger force resisting closure of the outlet valve than the force tending to open the outlet valve. The double reed valve enables engine operation at relatively higher torque and lower efficiency at low speed, with lower torque, but higher efficiency at high speed.

  7. Navy Breaks World Record With Futuristic Free-Electron Laser (FOX News.com)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    | Jefferson Lab Navy Breaks World Record With Futuristic Free-Electron Laser (FOX News.com) External Link: http://www.foxnews.com/scitech/2011/02/18/navy-breaks-world-record-futuristic-la... By jlab_admin on Sun, 2011-02-20

  8. Isospin-breaking two-nucleon force with explicit {delta} excitations

    SciTech Connect (OSTI)

    Epelbaum, E.; Meissner, Ulf-G.; Krebs, H.

    2008-03-15

    We study the leading isospin-breaking contributions to the two-nucleon two-pion exchange potential due to explicit {delta} degrees of freedom in chiral effective field theory. In particular, we find important contributions due to the delta mass splittings to the charge symmetry breaking potential that act opposite to the effects induced by the nucleon mass splitting.

  9. Break-Even Cost for Residential Photovoltaics in the United States: Key Drivers and Sensitivities

    SciTech Connect (OSTI)

    Denholm, P.; Margolis, R. M.; Ong, S.; Roberts, B.

    2009-12-01

    Grid parity--or break-even cost--for photovoltaic (PV) technology is defined as the point where the cost of PV-generated electricity equals the cost of electricity purchased from the grid. Break-even cost is expressed in $/W of an installed system. Achieving break-even cost is a function of many variables. Consequently, break-even costs vary by location and time for a country, such as the United States, with a diverse set of resources, electricity prices, and other variables. In this report, we analyze PV break-even costs for U.S. residential customers. We evaluate some key drivers of grid parity both regionally and over time. We also examine the impact of moving from flat to time-of-use (TOU) rates, and we evaluate individual components of the break-even cost, including effect of rate structure and various incentives. Finally, we examine how PV markets might evolve on a regional basis considering the sensitivity of the break-even cost to four major drivers: technical performance, financing parameters, electricity prices and rates, and policies. We find that local incentives rather than ?technical? parameters are in general the key drivers of the break-even cost of PV. Additionally, this analysis provides insight about the potential viability of PV markets.

  10. Can quantum coherent solar cells break detailed balance?

    SciTech Connect (OSTI)

    Kirk, Alexander P.

    2015-07-21

    Carefully engineered coherent quantum states have been proposed as a design attribute that is hypothesized to enable solar photovoltaic cells to break the detailed balance (or radiative) limit of power conversion efficiency by possibly causing radiative recombination to be suppressed. However, in full compliance with the principles of statistical mechanics and the laws of thermodynamics, specially prepared coherent quantum states do not allow a solar photovoltaic cell—a quantum threshold energy conversion device—to exceed the detailed balance limit of power conversion efficiency. At the condition given by steady-state open circuit operation with zero nonradiative recombination, the photon absorption rate (or carrier photogeneration rate) must balance the photon emission rate (or carrier radiative recombination rate) thus ensuring that detailed balance prevails. Quantum state transitions, entropy-generating hot carrier relaxation, and photon absorption and emission rate balancing are employed holistically and self-consistently along with calculations of current density, voltage, and power conversion efficiency to explain why detailed balance may not be violated in solar photovoltaic cells.

  11. Sea quark transverse momentum distributions and dynamical chiral symmetry breaking

    SciTech Connect (OSTI)

    Schweitzer, Peter; Strikman, Mark; Weiss, Christian

    2014-01-01

    Recent theoretical studies have provided new insight into the intrinsic transverse momentum distributions of valence and sea quarks in the nucleon at a low scale. The valence quark transverse momentum distributions (q - qbar) are governed by the nucleon's inverse hadronic size R{sup -1} ~ 0.2 GeV and drop steeply at large p{sub T}. The sea quark distributions (qbar) are in large part generated by non-perturbative chiral-symmetry breaking interactions and extend up to the scale rho{sup -1} ~ 0.6 GeV. These findings have many implications for modeling the initial conditions of perturbative QCD evolution of TMD distributions (starting scale, shape of p{sub T}. distributions, coordinate-space correlation functions). The qualitative difference between valence and sea quark intrinsic p{sub T}. distributions could be observed experimentally, by comparing the transverse momentum distributions of selected hadrons in semi-inclusive deep-inelastic scattering, or those of dileptons produced in pp and pbar-p scattering.

  12. Treatment methods for breaking certain oil and water emulsions

    DOE Patents [OSTI]

    Sealock, Jr., L. John; Baker, Eddie G.; Elliott, Douglas C.

    1992-01-01

    Disclosed are treatment methods for breaking emulsions of petroleum oil and salt water, fatty oil and water, and those resulting from liquefication of organic material. The emulsions are broken by heating to a predetermined temperature at or above about 200.degree. C. and pressurizing to a predetermined pressure above the vapor pressure of water at the predetermined temperature to produce a heated and pressurized fluid. The heated and pressurized fluid is contained in a single vessel at the predetermined temperature and pressure for a predetermined period of time to effectively separate the emulsion into substantially distinct first and second phases, the first phase comprising primarily the petroleum oil, the second phase comprising primarily the water. The first and second phases are separately withdrawn from the vessel at a withdraw temperature between about 200.degree. C. and 374.degree. C. and a withdraw pressure above the vapor pressure of water at the withdraw temperature. Where solids are present in the certain emulsions, the above described treatment may also effectively separate the certain emulsion into a substantially distinct third phase comprising primarily the solids.

  13. Crystal Structure of a Super Leucine Zipper an Extended Two-Stranded Super Long Coiled Coil

    SciTech Connect (OSTI)

    J Diao

    2011-12-31

    Coiled coil is a ubiquitous structural motif in proteins, with two to seven alpha helices coiled together like the strands of a rope, and coiled coil folding and assembly is not completely understood. A GCN4 leucine zipper mutant with four mutations of K3A, D7A, Y17W, and H18N has been designed, and the crystal structure has been determined at 1.6 {angstrom} resolution. The peptide monomer shows a helix trunk with short curved N- and C-termini. In the crystal, two monomers cross in 35{sup o} and form an X-shaped dimer, and each X-shaped dimer is welded into the next one through sticky hydrophobic ends, thus forming an extended two-stranded, parallel, super long coiled coil rather than a discrete, two-helix coiled coil of the wild-type GCN4 leucine zipper. Leucine residues appear at every seventh position in the super long coiled coil, suggesting that it is an extended super leucine zipper. Compared to the wild-type leucine zipper, the N-terminus of the mutant has a dramatic conformational change and the C-terminus has one more residue Glu 32 determined. The mutant X-shaped dimer has a large crossing angle of 35{sup o} instead of 18{sup o} in the wild-type dimer. The results show a novel assembly mode and oligomeric state of coiled coil, and demonstrate that mutations may affect folding and assembly of the overall coiled coil. Analysis of the formation mechanism of the super long coiled coil may help understand and design self-assembling protein fibers.

  14. SciTech Connect: "neutrinoless double beta decay"

    Office of Scientific and Technical Information (OSTI)

    neutrinoless double beta decay" Find + Advanced Search Term Search Semantic Search Advanced Search All Fields: "neutrinoless double beta decay" Semantic Semantic Term Title:...

  15. Double Shock Experiments and Reactive Flow Modeling of High Pressure...

    Office of Scientific and Technical Information (OSTI)

    Double Shock Experiments and Reactive Flow Modeling of High Pressure LX-17 Detonation Reaction Product States Citation Details In-Document Search Title: Double Shock Experiments ...

  16. Water-lithium bromide double-effect absorption cooling analysis...

    Office of Scientific and Technical Information (OSTI)

    Water-lithium bromide double-effect absorption cooling analysis Citation Details In-Document Search Title: Water-lithium bromide double-effect absorption cooling analysis You ...

  17. A Microscopic Double-Slit Experiment

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Microscopic Double-Slit Experiment Print Two centuries ago, Thomas Young performed the classic demonstration of the wave nature of light. He placed a screen with two tiny slits in...

  18. A Microscopic Double-Slit Experiment

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    A Microscopic Double-Slit Experiment Print Two centuries ago, Thomas Young performed the classic demonstration of the wave nature of light. He placed a screen with two tiny slits...

  19. Double bevel construction of a diamond anvil

    DOE Patents [OSTI]

    Moss, W.C.

    1987-02-06

    Use of double or multiple bevel culet geometry on a diamond anvil to provide increased sample pressure and stability for a given force applied to the diamond tables. 7 figs.

  20. Double logarithmic asymptotic behavior in quantum chromodynamics

    SciTech Connect (OSTI)

    Kirschner, R.

    1981-08-01

    The double logarithmic contributions to the quark-(anti)quark scattering and annihilation amplitudes are summed to all orders in quantum chromodynamics. The results are a generalization of the calculations of Gorshkov et al. in the case of quantum electrodynamics.

  1. Double shell tank waste analysis plan

    SciTech Connect (OSTI)

    Mulkey, C.H.; Jones, J.M.

    1994-12-15

    Waste analysis plan for the double shell tanks. SD-WM-EV-053 is Superseding SD-WM-EV-057.This document provides the plan for obtaining information needed for the safe waste handling and storage of waste in the Double Shell Tank Systems. In Particular it addresses analysis necessary to manage waste according to Washington Administrative Code 173-303 and Title 40, parts 264 and 265 of the Code of Federal Regulations.

  2. Wavelength-doubling optical parametric oscillator

    DOE Patents [OSTI]

    Armstrong, Darrell J.; Smith, Arlee V.

    2007-07-24

    A wavelength-doubling optical parametric oscillator (OPO) comprising a type II nonlinear optical medium for generating a pair of degenerate waves at twice a pump wavelength and a plurality of mirrors for rotating the polarization of one wave by 90 degrees to produce a wavelength-doubled beam with an increased output energy by coupling both of the degenerate waves out of the OPO cavity through the same output coupler following polarization rotation of one of the degenerate waves.

  3. JET COLLIMATION IN THE EJECTA OF DOUBLE NEUTRON STAR MERGERS: A NEW CANONICAL PICTURE OF SHORT GAMMA-RAY BURSTS

    SciTech Connect (OSTI)

    Nagakura, Hiroki; Sekiguchi, Yuichiro; Shibata, Masaru; Hotokezaka, Kenta; Ioka, Kunihito

    2014-04-01

    The observations of jet breaks in the afterglows of short gamma-ray bursts (SGRBs) indicate that the jet has a small opening angle of ? 10. The collimation mechanism of the jet is a longstanding theoretical problem. We numerically analyze the jet propagation in the material ejected by a double neutron star (NS) merger, and demonstrate that if the ejecta mass is ? 10{sup 2} M {sub ?}, the jet is well confined by the cocoon and emerges from the ejecta with the required collimation angle. Our results also suggest that there are some populations of choked (failed) SGRBs or new types of events with low luminosity. By constructing a model for SGRB 130603B, which is associated with the first kilonova/macronova candidate, we infer that the equation of state of NSs would be soft enough to provide sufficient ejecta to collimate the jet, if this event is associated with a double NS merger.

  4. Particle-Hole Symmetry Breaking in the Pseudogap State of Bi2201

    SciTech Connect (OSTI)

    Hashimoto, M.; He, R.-H.; Tanaka, K.; Testaud, J.P.; Meevasana1, W.; Moore, R.G.; Lu, D.H.; Yao, H.; Yoshida, Y.; Eisaki, H.; Devereaux, T.P.; Hussain, Z.; Shen, Z.-X.; /SIMES, Stanford /Stanford U., Geballe Lab.

    2011-08-19

    In conventional superconductors, a gap exists in the energy absorption spectrum only below the transition temperature (T{sub c}), corresponding to the energy price to pay for breaking a Cooper pair of electrons. In high-T{sub c} cuprate superconductors above T{sub c}, an energy gap called the pseudogap exists, and is controversially attributed either to pre-formed superconducting pairs, which would exhibit particle-hole symmetry, or to competing phases which would typically break it. Scanning tunnelling microscopy (STM) studies suggest that the pseudogap stems from lattice translational symmetry breaking and is associated with a different characteristic spectrum for adding or removing electrons (particle-hole asymmetry). However, no signature of either spatial or energy symmetry breaking of the pseudogap has previously been observed by angle-resolved photoemission spectroscopy (ARPES). Here we report ARPES data from Bi2201 which reveals both particle-hole symmetry breaking and dramatic spectral broadening indicative of spatial symmetry breaking without long range order, upon crossing through T* into the pseudogap state. This symmetry breaking is found in the dominant region of the momentum space for the pseudogap, around the so-called anti-node near the Brillouin zone boundary. Our finding supports the STM conclusion that the pseudogap state is a broken-symmetry state that is distinct from homogeneous superconductivity.

  5. A fair compromise to break the climate impasse. A major economies forum approach to emissions reductions budgeting

    SciTech Connect (OSTI)

    Grasso, Marco; J. Roberts, Timmons

    2013-04-15

    Key messages of the study are: Given the stalemate in U.N. climate negotiations, the best arena to strike a workable deal is among the members the Major Economies Forum on Energy and Climate (MEF); The 13 MEF members—including the EU-27 (but not double-counting the four EU countries that are also individual members of the MEF)—account for 81.3 percent of all global emissions; This proposal devises a fair compromise to break the impasse to develop a science-based approach for fairly sharing the carbon budget in order to have a 75 percent chance of avoiding dangerous climate change; To increase the likelihood of a future climate agreement, carbon accounting must shift from production-based inventories to consumption-based ones; The shares of a carbon budget to stay below 2 deg C through 2050 are calculated by cumulative emissions since 1990, i.e. according to a short-horizon polluter pays principle, and national capability (income), and allocated to MEF members through emission rights. This proposed fair compromise addresses key concerns of major emitters; According to this accounting, no countries have negative carbon budgets, there is substantial time for greening major developing economies, and some developed countries need to institute very rapid reductions in emissions; and, To provide a 'green ladder' to developing countries and to ensure a fair global deal, it will be crucial to agree how to extend sufficient and predictable financial support and the rapid transfer of technology.

  6. TRAC-PF1/MOD1 analysis of a 200% cold-leg break in a US/Japanese PWR with four loops and 15 x 15 fuel

    SciTech Connect (OSTI)

    Spore, J.W.; Cappiello, M.W.

    1986-01-01

    This report presents the results of a TRAC-PF1/MOD1 calculation that simulated a 200% double-ended cold-leg-break loss-of-coolant accident in a generic US/Japanese pressurized water reactor. This is a best-estimate analysis using conservative boundary conditions and minimum safeguards. The calculation shows that the peak cladding temperature (PCT) occurs during blowdown and that the core reheat is minimal during reflood. The results also show that for an evaluation-model peak rod linear power of 15.85 kW/ft, a PCT of 1084 K is reached at 3.5 s into the blowdown transient, which is approx.394 K below the design basis limit of 1478 K. 10 figs.

  7. U.S. Department of Energy Breaks Ground on State-of-the-Art Wind Turbine

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Test Facility | Department of Energy Breaks Ground on State-of-the-Art Wind Turbine Test Facility U.S. Department of Energy Breaks Ground on State-of-the-Art Wind Turbine Test Facility October 1, 2012 - 12:08pm Addthis This is an excerpt from the Third Quarter 2012 edition of the Wind Program R&D Newsletter. The U.S. Department of Energy (DOE) joined with Texas Tech University (TTU) and Sandia National Laboratories (SNL) in July 2012 to break ground on a new state-of-the-art wind turbine

  8. Dynamics of Peccei-Quinn breaking field after inflation and axion isocurvature perturbations

    SciTech Connect (OSTI)

    Harigaya, Keisuke; Ibe, Masahiro; Kawasaki, Masahiro; Yanagida, Tsutomu T.

    2015-11-04

    The Peccei-Quinn mechanism suffers from the problem of the isocurvature perturbations. The isocurvature perturbations are suppressed if the Peccei-Quinn breaking scale is large during inflation. The oscillation of the Peccei-Quinn breaking field after inflation, however, leads to the formation of domain walls due to the parametric resonance effect. In this paper, we discuss the evolution of the Peccei-Quinn breaking field after inflation in detail, and propose a model where the parametric resonance is ineffective and hence domain walls are not formed. We also discuss consistency of our model with supersymmetric theory.

  9. Photo of the Week: Record-Breaking Solar Cells | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Record-Breaking Solar Cells Photo of the Week: Record-Breaking Solar Cells December 7, 2012 - 2:27pm Addthis Solar Junction, in partnership with NREL, has developed solar cells that reach a record-breaking 44 percent efficiency -- meaning that more than 40 percent of the sunlight the solar cells are exposed to is converted into electrical energy. In this photo, an operator inspects a photolithography tool used to manufacture these solar cells. | Photo by Daniel Derkacs/SolarJunction. Solar

  10. Pair correlations in neutrinoless double {beta} decay candidate {sup 130}Te.

    SciTech Connect (OSTI)

    Bloxham, T.; Kay, B. P.; Schiffer, J. P.; Clark, J. A.; Deibel, C. M.; Freeman, S. J.; Freedman, S. J.; Howard, A. M.; McAllister, S. A.; Parker, P. D.; Sharp, D. K.; Thomas, J. S. (Physics); ( PSC-USR); (LBNL); (Michigan State Univ.); (Univ. of Manchester); (Yale Univ.)

    2010-08-16

    Pair correlations in the ground state of {sup 130}Te have been investigated using pair-transfer experiments to explore the validity of approximations in calculating the matrix element for neutrinoless double-{beta} decay. This nucleus is a candidate for the observation of such decay, and a good understanding of its structure is crucial for eventual calculations of the neutrino mass, should such a decay indeed be observed. For proton-pair adding, strong transitions to excited 0{sup +} states had been observed in the Te isotopes by Alford et al. [Nucl. Phys. A 323, 339 (1979)], indicating a breaking of the BCS approximation for protons in the ground state. We measured the neutron-pair removing (p,t) reaction on {sup 130}Te and found no indication of a corresponding splitting of the BCS nature of the ground state for neutrons.

  11. Can one measure nuclear matrix elements of neutrinoless double {beta} decay?

    SciTech Connect (OSTI)

    Rodin, Vadim; Faessler, Amand [Institute fuer Theoretische Physik der Universitaet Tuebingen, D-72076 Tuebingen (Germany)

    2009-10-15

    By making use of the isospin conservation by strong interaction, the Fermi 0{nu}{beta}{beta} nuclear matrix element M{sub F}{sup 0{nu}} is transformed to acquire the form of an energy-weighted double Fermi transition matrix element. This useful representation allows reconstruction of the total M{sub F}{sup 0{nu}} provided a small isospin-breaking Fermi matrix element between the isobaric analog state in the intermediate nucleus and the ground state of the daughter nucleus could be measured, e.g., by charge-exchange reactions. Such a measurement could set a scale for the 0{nu}{beta}{beta} nuclear matrix elements and help to discriminate between the different nuclear structure models in which calculated M{sub F}{sup 0{nu}} may differ by as much as a factor of 5 (that translates to about 20% difference in the total M{sup 0{nu}})

  12. Pair correlations in the neutrinoless double-{beta} decay candidate {sup 130}Te

    SciTech Connect (OSTI)

    Bloxham, T.; Freedman, S. J. [Lawrence Berkeley National Laboratory, Berkeley, California 94720 (United States); Kay, B. P.; Schiffer, J. P.; Clark, J. A. [Physics Division, Argonne National Laboratory, Argonne, Illinois 60439 (United States); Deibel, C. M. [Physics Division, Argonne National Laboratory, Argonne, Illinois 60439 (United States); Joint Institute for Nuclear Astrophysics, Michigan State University, East Lansing, Michigan 48825 (United States); Freeman, S. J.; Howard, A. M.; McAllister, S. A.; Sharp, D. K.; Thomas, J. S. [Schuster Laboratory, University of Manchester, Manchester, M13 9PL (United Kingdom); Parker, P. D. [A. W. Wright Nuclear Structure Laboratory, Yale University, New Haven, Connecticut 06520 (United States)

    2010-08-15

    Pair correlations in the ground state of {sup 130}Te have been investigated using pair-transfer experiments to explore the validity of approximations in calculating the matrix element for neutrinoless double-{beta} decay. This nucleus is a candidate for the observation of such decay, and a good understanding of its structure is crucial for eventual calculations of the neutrino mass, should such a decay indeed be observed. For proton-pair adding, strong transitions to excited 0{sup +} states had been observed in the Te isotopes by Alford et al. [Nucl. Phys. A 323, 339 (1979)], indicating a breaking of the BCS approximation for protons in the ground state. We measured the neutron-pair removing (p,t) reaction on {sup 130}Te and found no indication of a corresponding splitting of the BCS nature of the ground state for neutrons.

  13. Disentangling the various Mechanisms of neutrinoless double beta decay to extract the neutrino mass

    SciTech Connect (OSTI)

    Vergados, J. D. [Physics Department, University of Ioannina, Ioannina, GR 451 10, Greece and Theory Division, CERN 1211, Geneva 23 (Greece)

    2011-12-16

    It is well known that there exist many mechanisms that may contribute to neutrinoless double beta decay. By exploiting the fact that the associated nuclear matrix elements are target dependent we show that, given definite experimental results on a sufficient number of targets, one can determine or sufficiently constrain all lepton violating parameters including the mass term. As a specific example we show that, given the observation of the 0{nu}{beta}{beta}-decay in three different nuclei, e.g. {sup 76}Ge, {sup 100}Mo and {sup 130}Te, and assuming just three active lepton number violating parameters, e.g. light and heavy neutrino mass mechanisms in left handed currents as well as R-parity breaking SUSY mechanism, one may determine all lepton violating parameters, provided that they are relatively real.

  14. Direct link between neutrinoless double beta decay and leptogenesis in a seesaw model with S{sub 4} symmetry

    SciTech Connect (OSTI)

    Ahn, Y. H. [Institute of Physics, Academia Sinica, Taipei 115, Taiwan (China); Kang, Sin Kyu [School of Liberal Arts, Seoul National Univ. of Technology, Seoul 139-743 (Korea, Republic of); Kim, C. S. [Department of Physics and IPAP, Yonsei University, Seoul 120-749 (Korea, Republic of); Nguyen, T. Phong [Department of Physics and IPAP, Yonsei University, Seoul 120-749 (Korea, Republic of); Department of Physics, Cantho University, Cantho (Viet Nam)

    2010-11-01

    We study how leptogenesis can be implemented in a seesaw model with S{sub 4} flavor symmetry, which leads to the neutrino tribimaximal mixing matrix and degenerate right-handed (RH) neutrino spectrum. Introducing a tiny soft S{sub 4} symmetry breaking term in the RH neutrino mass matrix, we show that the flavored resonant leptogenesis can be successfully realized, which can lower the seesaw scale much so, as to make it possible to probe in colliders. Even though such a tiny soft breaking term is essential for leptogenesis, it does not significantly affect the low-energy observables. We also investigate how the effective light neutrino mass || associated with neutrinoless double beta decay can be predicted along with the neutrino mass hierarchies by imposing experimental data of low-energy observables. We find a direct link between leptogenesis and neutrinoless double beta decay characterized by || through a high energy CP phase {phi}, which is correlated with low-energy Majorana CP phases. It is shown that our predictions of || for some fixed parameters of high energy physics can be constrained by the current observation of baryon asymmetry.

  15. San Carlos Apache Tribe Set to Break Ground on New Solar Project...

    Energy Savers [EERE]

    This spring, the San Carlos Apache Tribe is planning to break ground on a new tribally financed and owned 1.1-megawatt (MW) solar photovoltaic (PV) array. The PV system will ...

  16. Introduction of Break-Out Session at the International PV Module Quality Assurance Forum (Presentation)

    SciTech Connect (OSTI)

    Kurtz, S.; Wohlgemuth, J.; Yamamichi, M.; Sample, T.

    2011-07-01

    This presentation outlines review requirements for quality assurance (QA) rating systems, logical design of QA systems, and specific tasks for break-out session 1 of the 2011 International PV Module Quality Assurance Forum.

  17. Dynamics of Peccei-Quinn breaking field after inflation and axion...

    Office of Scientific and Technical Information (OSTI)

    In this paper, we discuss the evolution of the Peccei-Quinn breaking field after inflation in detail, and propose a model where the parametric resonance is ineffective and hence ...

  18. Fermi-LAT Detection of a Break in the Gamma-Ray Spectrum of the...

    Office of Scientific and Technical Information (OSTI)

    in the Gamma-Ray Spectrum of the Supernova Remnant Cassiopeia A Citation Details In-Document Search Title: Fermi-LAT Detection of a Break in the Gamma-Ray Spectrum of the Supernova ...

  19. Detection of a Spectral Break in the Extra Hard Component of...

    Office of Scientific and Technical Information (OSTI)

    the Extra Hard Component of GRB 090926A Citation Details In-Document Search Title: Detection of a Spectral Break in the Extra Hard Component of GRB 090926A You are accessing a ...

  20. Strand-Specific RNA-Seq Analyses of Fruiting Body Development in Coprinopsis cinerea

    SciTech Connect (OSTI)

    Muraguchi, Hajime; Umezawa, Kiwamu; Niikura, Mai; Yoshida, Makoto; Kozaki, Toshinori; Ishii, Kazuo; Sakai, Kiyota; Shimizu, Motoyuki; Nakahori, Kiyoshi; Sakamoto, Yuichi; Choi, Cindy; Ngan, Chew Yee; Lindquist, Eika; Lipzen, Anna; Tritt, Andrew; Haridas, Sajeet; Barry, Kerrie; Grigoriev, Igor V.; Pukkila, Patricia J.

    2015-10-28

    We report that the basidiomycete fungus Coprinopsis cinerea is an important model system for multicellular development. Fruiting bodies of C. cinerea are typical mushrooms, which can be produced synchronously on defined media in the laboratory. To investigate the transcriptome in detail during fruiting body development, high-throughput sequencing (RNA-seq) was performed using cDNA libraries strand-specifically constructed from 13 points (stages/tissues) with two biological replicates. The reads were aligned to 14,245 predicted transcripts, and counted for forward and reverse transcripts. Differentially expressed genes (DEGs) between two adjacent points and between vegetative mycelium and each point were detected by Tag Count Comparison (TCC). To validate RNA-seq data, expression levels of selected genes were compared using RPKM values in RNA-seq data and qRT-PCR data, and DEGs detected in microarray data were examined in MA plots of RNA-seq data by TCC. We discuss events deduced from GO analysis of DEGs. In addition, we uncovered both transcription factor candidates and antisense transcripts that are likely to be involved in developmental regulation for fruiting.

  1. Strand-Specific RNA-Seq Analyses of Fruiting Body Development in Coprinopsis cinerea

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Muraguchi, Hajime; Umezawa, Kiwamu; Niikura, Mai; Yoshida, Makoto; Kozaki, Toshinori; Ishii, Kazuo; Sakai, Kiyota; Shimizu, Motoyuki; Nakahori, Kiyoshi; Sakamoto, Yuichi; et al

    2015-10-28

    We report that the basidiomycete fungus Coprinopsis cinerea is an important model system for multicellular development. Fruiting bodies of C. cinerea are typical mushrooms, which can be produced synchronously on defined media in the laboratory. To investigate the transcriptome in detail during fruiting body development, high-throughput sequencing (RNA-seq) was performed using cDNA libraries strand-specifically constructed from 13 points (stages/tissues) with two biological replicates. The reads were aligned to 14,245 predicted transcripts, and counted for forward and reverse transcripts. Differentially expressed genes (DEGs) between two adjacent points and between vegetative mycelium and each point were detected by Tag Count Comparison (TCC).more » To validate RNA-seq data, expression levels of selected genes were compared using RPKM values in RNA-seq data and qRT-PCR data, and DEGs detected in microarray data were examined in MA plots of RNA-seq data by TCC. We discuss events deduced from GO analysis of DEGs. In addition, we uncovered both transcription factor candidates and antisense transcripts that are likely to be involved in developmental regulation for fruiting.« less

  2. Laminar burn rates of gun propellants measured in the high-pressure strand burner

    SciTech Connect (OSTI)

    Reaugh, J. E., LLNL

    1997-10-01

    The pressure dependence of the laminar burn rate of gun propellants plays a role in the design and behavior of high-performance guns. We have begun a program to investigate the effects of processing variables on the laminar burn rates, using our high-pressure strand burner to measure these rates at pressures exceeding 700 MPa. We have burned JA2 and M43 propellant samples, provided by Dr. Arpad Juhasz, ARL, from propellant lots previously used in round-robin tests. Our results at room temperature are in accord with other measurements. In addition, we present results measured for propellant that has been preheated to 50 C before burning. We used our thermochemical equilibrium code, CHEETAH, to help interpret the simultaneous pressure and temperature measurements taken during the testing, and show examples of its use. It has been modified to provide performance measures and equations of state for the products that are familiar to the gun-propellant community users of BLAKE.

  3. Energy Department Announces $12 Million to Accelerate Record-Breaking Solar

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Cell Efficiency | Department of Energy 2 Million to Accelerate Record-Breaking Solar Cell Efficiency Energy Department Announces $12 Million to Accelerate Record-Breaking Solar Cell Efficiency January 25, 2013 - 3:41pm Addthis As part of the Energy Department's SunShot Initiative, the Department today announced $12 million to develop innovative, ultra-efficient solar devices that will close the gap with the theoretical efficiency limit, or the highest potential percentage of sunlight

  4. More than $3 million raised during record-breaking Employee Giving Campaign

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Record-breaking employee giving campaign Community Connections: Your link to news and opportunities from Los Alamos National Laboratory Latest Issue: September 1, 2016 all issues All Issues » submit More than $3 million raised during record-breaking Employee Giving Campaign Contributions will benefit regional nonprofits December 1, 2014 Mobile medical and dental clinic owned by Tierra Amarillas' La Clinica del Pueblo de Rio Arriba. The purchase of the vehicle was made possible by donations

  5. Breaking Up (Hydrogen) No Longer As Hard To Do | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Breaking Up (Hydrogen) No Longer As Hard To Do Breaking Up (Hydrogen) No Longer As Hard To Do December 29, 2011 - 1:12pm Addthis Researchers at Argonne National Lab have recently developed a process to improve the efficiency of producing hydrogen to run cars such as this prototype, which was developed at the Oakridge National Lab. | Photo courtesy of Oak Ridge National Laboratory. Researchers at Argonne National Lab have recently developed a process to improve the efficiency of producing

  6. Novel Thermal Break with Simplified Manufacturing for R7 Commercial Windows

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    | Department of Energy Thermal Break with Simplified Manufacturing for R7 Commercial Windows Novel Thermal Break with Simplified Manufacturing for R7 Commercial Windows Image courtesy of Alcoa and BTO Peer Review. Image courtesy of Alcoa and BTO Peer Review. Lead Performer: Alcoa - Pittsburgh, PA DOE Funding: $1,123,838 Cost Share: $280,960 Project Term: October 2014 - September 2016 Funding Opportunity: Building Energy Efficiency Frontiers and Incubator Technologies (BENEFIT) - 2014

  7. Metallic glass could make your next cell phone harder to break

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Metallic glass could make your next cell phone harder to break Alumni Link: Opportunities, News and Resources for Former Employees Latest Issue:September 2015 all issues All Issues » submit Metallic glass could make your next cell phone harder to break Lab researcher works to rearrange the atoms in metals June 20, 2014 New insights to changing the atomic structure of metals New insights to changing the atomic structure of metals Contact Linda Anderman Email Metal and glass objects are all

  8. Helping STEM Education Take Root: Breaking Ground on a Field Station in

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Indian Country | Department of Energy Helping STEM Education Take Root: Breaking Ground on a Field Station in Indian Country Helping STEM Education Take Root: Breaking Ground on a Field Station in Indian Country November 7, 2012 - 10:50am Addthis (From Left) Stuart Harris, Confederated Tribes of the Umatilla Indian Reservation, Director of the Department of Science and Engineering, Dave Huizenga, Senior Advisor for the Office of Environmental Management, and Tracey LeBeau, Director of the

  9. Record-breaking year for patents | Y-12 National Security Complex

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Record-breaking year for ... Record-breaking year for patents Posted: February 11, 2013 - 2:57pm | Y-12 Report | Volume 9, Issue 2 | 2013 Fiscal 2012 was a fruitful year for innovative research and development at the Y-12 National Security Complex. Site inventors were issued an unprecedented 11 patents. Technologies and processes from many of Y-12's latest patents, like those from previous years, can extend beyond the Nuclear Security Enterprise into diverse areas of private industry. One

  10. Breaking Ground and Driving Discovery | U.S. DOE Office of Science (SC)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Breaking Ground and Driving Discovery News News Home Featured Articles 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 Science Headlines Science Highlights Presentations & Testimony News Archives Communications and Public Affairs Contact Information Office of Science U.S. Department of Energy 1000 Independence Ave., SW Washington, DC 20585 P: (202) 586-5430 12.16.11 Breaking Ground and Driving Discovery Director Brinkman welcomes the Illinois Accelerator Research Center. Print

  11. Breaking Up - And Making Up - Are Hard To Do | U.S. DOE Office of

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Breaking Up (Hydrogen) No Longer As Hard To Do Breaking Up (Hydrogen) No Longer As Hard To Do December 29, 2011 - 1:12pm Addthis Researchers at Argonne National Lab have recently developed a process to improve the efficiency of producing hydrogen to run cars such as this prototype, which was developed at the Oakridge National Lab. | Photo courtesy of Oak Ridge National Laboratory. Researchers at Argonne National Lab have recently developed a process to improve the efficiency of producing

  12. Bubbles Help Break Energy Storage Record for Lithium Air-Batteries

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Bubbles Help Break Energy Storage Record for Lithium Air-Batteries Bubbles Help Break Energy Storage Record for Lithium Air-Batteries Foam-base graphene keeps oxygen flowing in batteries that holds promise for electric vehicles January 25, 2012 Linda Vu, lvu@lbl.gov, +1 510 495 2402 Using a new approach, the team built a graphene membrane for use in lithium-air batteries, which could, one day, replace conventional batteries in electric vehicles. Resembling coral, this porous graphene material

  13. BLOCKING OSCILLATOR DOUBLE PULSE GENERATOR CIRCUIT

    DOE Patents [OSTI]

    Haase, J.A.

    1961-01-24

    A double-pulse generator, particuiarly a double-pulse generator comprising a blocking oscillator utilizing a feedback circuit to provide means for producing a second pulse within the recovery time of the blocking oscillator, is described. The invention utilized a passive network which permits adjustment of the spacing between the original pulses derived from the blocking oscillator and further utilizes the original pulses to trigger a circuit from which other pulses are initiated. These other pulses are delayed and then applied to the input of the blocking oscillator, with the result that the output from the oscillator circuit contains twice the number of pulses originally initiated by the blocking oscillator itself.

  14. A Microscopic Double-Slit Experiment

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    A Microscopic Double-Slit Experiment A Microscopic Double-Slit Experiment Print Wednesday, 29 February 2012 00:00 Two centuries ago, Thomas Young performed the classic demonstration of the wave nature of light. He placed a screen with two tiny slits in front of a single light source, effectively converting it into a two-centered source. On a second screen far away, he saw a pattern of light and dark diffraction fringes, a well-known hallmark of wave interference. Along with later studies using

  15. Searches for Neutrino-less Double

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Searches for Neutrino-less Double Beta-Decay: a Decade of Discovery Ahead at the ton-scale? David Nygren University of Texas at Arlington September 2, 2015 4:00 p.m. Double beta-decay with the emission of neutrinos is an allowed second-order process and has been observed in several candidate nuclei such as 76Ge, 82Se, and 136Xe. A neutrino-less decay mode is theoretically possible as well, with profound implications such as non-conservation of lepton number. The neutrino-less decay can only

  16. The double-beta decay: Theoretical challenges

    SciTech Connect (OSTI)

    Horoi, Mihai

    2012-11-20

    Neutrinoless double beta decay is a unique process that could reveal physics beyond the Standard Model of particle physics namely, if observed, it would prove that neutrinos are Majorana particles. In addition, it could provide information regarding the neutrino masses and their hierarchy, provided that reliable nuclear matrix elements can be obtained. The two neutrino double beta decay is an associate process that is allowed by the Standard Model, and it was observed for about ten nuclei. The present contribution gives a brief review of the theoretical challenges associated with these two process, emphasizing the reliable calculation of the associated nuclear matrix elements.

  17. On the neutrinoless double ?{sup +}/EC decays

    SciTech Connect (OSTI)

    Suhonen, Jouni

    2013-12-30

    The neutrinoless double positron-emission/electron-capture (0??{sup +}/EC) decays are studied for the magnitudes of the involved nuclear matrix elements (NMEs). Decays to the ground state, 0{sub gs}{sup +}, and excited 0{sup +} states are discussed. The participant many-body wave functions are evaluated in the framework of the quasiparticle random-phase approximation (QRPA). Effective, G-matrix-derived nuclear forces are used in realistic single-particle model spaces. The channels ?{sup +}?{sup +}, ?{sup +}EC, and the resonant neutrinoless double electron capture (R0?ECEC) are discussed.

  18. Double layer capacitor prospects look good

    SciTech Connect (OSTI)

    1995-07-01

    The Fourth International Seminar in Double Layer Capacitors and similar energy devices has been sponsored again by Dr. S.P. Wolsky and Dr. Nikola Marincic. The seminar was held in December 1994, at Deerfield Beach, FL. This report provides a brief description of information on supercapacitors.

  19. The Promiscuity of [beta]-Strand Pairing Allows for Rational Design of [beta]-Sheet Face Inversion

    SciTech Connect (OSTI)

    Makabe, Koki; Koide, Shohei

    2009-06-17

    Recent studies suggest the dominant role of main-chain H-bond formation in specifying {beta}-sheet topology. Its essentially sequence-independent nature implies a large degree of freedom in designing {beta}-sheet-based nanomaterials. Here we show rational design of {beta}-sheet face inversions by incremental deletions of {beta}-strands from the single-layer {beta}-sheet of Borrelia outer surface protein A. We show that a {beta}-sheet structure can be maintained when a large number of native contacts are removed and that one can design large-scale conformational transitions of a {beta}-sheet such as face inversion by exploiting the promiscuity of strand-strand interactions. High-resolution X-ray crystal structures confirmed the success of the design and supported the importance of main-chain H-bonds in determining {beta}-sheet topology. This work suggests a simple but effective strategy for designing and controlling nanomaterials based on {beta}-rich peptide self-assemblies.

  20. Solid-state NMR analysis of the {beta}-strand orientation of the protofibrils of amyloid {beta}-protein

    SciTech Connect (OSTI)

    Doi, Takashi; Masuda, Yuichi; Irie, Kazuhiro; Akagi, Ken-ichi; Monobe, Youko; Imazawa, Takayoshi; Takegoshi, K.

    2012-11-30

    Highlights: Black-Right-Pointing-Pointer The supramolecular structure of A{beta}42 protofibrils was analyzed by solid-state NMR. Black-Right-Pointing-Pointer The Ala-21 residue in the A{beta}42 protofibrils is included in a slightly disordered {beta}-strand. Black-Right-Pointing-Pointer The A{beta}42 protofibrils do not form intermolecular in-register parallel {beta}-sheets. -- Abstract: Alzheimer's disease (AD) is caused by abnormal deposition (fibrillation) of a 42-residue amyloid {beta}-protein (A{beta}42) in the brain. During the process of fibrillation, the A{beta}42 takes the form of protofibrils with strong neurotoxicity, and is thus believed to play a crucial role in the pathogenesis of AD. To elucidate the supramolecular structure of the A{beta}42 protofibrils, the intermolecular proximity of the Ala-21 residues in the A{beta}42 protofibrils was analyzed by {sup 13}C-{sup 13}C rotational resonance experiments in the solid state. Unlike the A{beta}42 fibrils, an intermolecular {sup 13}C-{sup 13}C correlation was not found in the A{beta}42 protofibrils. This result suggests that the {beta}-strands of the A{beta}42 protofibrils are not in an in-register parallel orientation. A{beta}42 monomers would assemble to form protofibrils with the {beta}-strand conformation, then transform into fibrils by forming intermolecular parallel {beta}-sheets.

  1. Multicopy single-stranded DNA directs intestinal colonization of enteric pathogens

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Elfenbein, Johanna R.; Knodler, Leigh A.; Nakayasu, Ernesto S.; Ansong, Charles; Brewer, Heather M.; Bogomolnaya, Lydia; Adams, L. Garry; McClelland, Michael; Adkins, Joshua N.; Andrews-Polymenis, Helene L.; et al

    2015-09-14

    Multicopy single-stranded DNAs (msDNAs) are hybrid RNA-DNA molecules encoded on retroelements called retrons and produced by the action of retron reverse transcriptases. Retrons are widespread in bacteria but the natural function of msDNA has remained elusive despite 30 years of study. The major roadblock to elucidation of the function of these unique molecules has been the lack of any identifiable phenotypes for mutants unable to make msDNA. We report that msDNA of the zoonotic pathogen Salmonella Typhimurium is necessary for colonization of the intestine. Similarly, we observed a defect in intestinal persistence in an enteropathogenic E. coli mutant lacking itsmore » retron reverse transcriptase. Under anaerobic conditions in the absence of msDNA, proteins of central anaerobic metabolism needed for Salmonella colonization of the intestine are dysregulated. We show that the msDNA-deficient mutant can utilize nitrate, but not other alternate electron acceptors in anaerobic conditions. Consistent with the availability of nitrate in the inflamed gut, a neutrophilic inflammatory response partially rescued the ability of a mutant lacking msDNA to colonize the intestine. These findings together indicate that the mechanistic basis of msDNA function during Salmonella colonization of the intestine is proper production of proteins needed for anaerobic metabolism. We further conclude that a natural function of msDNA is to regulate protein abundance, the first attributable function for any msDNA. Our data provide novel insight into the function of this mysterious molecule that likely represents a new class of regulatory molecules.« less

  2. Multicopy single-stranded DNA directs intestinal colonization of enteric pathogens

    SciTech Connect (OSTI)

    Elfenbein, Johanna R.; Knodler, Leigh A.; Nakayasu, Ernesto S.; Ansong, Charles; Brewer, Heather M.; Bogomolnaya, Lydia; Adams, L. Garry; McClelland, Michael; Adkins, Joshua N.; Andrews-Polymenis, Helene L.; Fang, Ferric C.

    2015-09-14

    Multicopy single-stranded DNAs (msDNAs) are hybrid RNA-DNA molecules encoded on retroelements called retrons and produced by the action of retron reverse transcriptases. Retrons are widespread in bacteria but the natural function of msDNA has remained elusive despite 30 years of study. The major roadblock to elucidation of the function of these unique molecules has been the lack of any identifiable phenotypes for mutants unable to make msDNA. We report that msDNA of the zoonotic pathogen Salmonella Typhimurium is necessary for colonization of the intestine. Similarly, we observed a defect in intestinal persistence in an enteropathogenic E. coli mutant lacking its retron reverse transcriptase. Under anaerobic conditions in the absence of msDNA, proteins of central anaerobic metabolism needed for Salmonella colonization of the intestine are dysregulated. We show that the msDNA-deficient mutant can utilize nitrate, but not other alternate electron acceptors in anaerobic conditions. Consistent with the availability of nitrate in the inflamed gut, a neutrophilic inflammatory response partially rescued the ability of a mutant lacking msDNA to colonize the intestine. These findings together indicate that the mechanistic basis of msDNA function during Salmonella colonization of the intestine is proper production of proteins needed for anaerobic metabolism. We further conclude that a natural function of msDNA is to regulate protein abundance, the first attributable function for any msDNA. Our data provide novel insight into the function of this mysterious molecule that likely represents a new class of regulatory molecules.

  3. Double, Double Toil and Trouble: Tungsten Burns and Helium Bubbles | U.S.

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    DOE Office of Science (SC) Double, Double Toil and Trouble: Tungsten Burns and Helium Bubbles Advanced Scientific Computing Research (ASCR) ASCR Home About Research Facilities Science Highlights Benefits of ASCR Funding Opportunities Advanced Scientific Computing Advisory Committee (ASCAC) Community Resources Contact Information Advanced Scientific Computing Research U.S. Department of Energy SC-21/Germantown Building 1000 Independence Ave., SW Washington, DC 20585 P: (301) 903-7486 F: (301)

  4. Symmetry breaking indication for supergravity inflation in light of the Planck 2015

    SciTech Connect (OSTI)

    Li, Tianjun; Li, Zhijin; Nanopoulos, Dimitri V.

    2015-09-01

    Supergravity (SUGRA) theories with exact global U(1) symmetry or shift symmetry in Kähler potential provide natural frameworks for inflation. However, quadratic inflation is disfavoured by the new results on primordial tensor fluctuations from the Planck Collaboration. To be consistent with the new Planck data, we point out that the explicit symmetry breaking is needed, and study these two SUGRA inflation in detail. For SUGRA inflation with global U(1) symmetry, the symmetry breaking term leads to a trigonometric modulation on inflaton potential. Coefficient of the U(1) symmetry breaking term is of order 10{sup −2}, which is sufficient large to improve the inflationary predictions while its higher order corrections are negligible. Such models predict sizeable tensor fluctuations and highly agree with the Planck results. In particular, the model with a linear U(1) symmetry breaking term predicts the tensor-to-scalar ratio around r∼0.01 and running spectral index α{sub s}∼−0.004, which comfortably fit with the Planck observations. For SUGRA inflation with breaking shift symmetry, the inflaton potential is modulated by an exponential factor. The modulated linear and quadratic models are consistent with the Planck observations. In both types of models the tensor-to-scalar ratio can be of order 10{sup −2}, which will be tested by the near future observations.

  5. The evolution of the break preclusion concept for nuclear power plants in Germany

    SciTech Connect (OSTI)

    Schulz, H.

    1997-04-01

    In the updating of the Guidelines for PWR`s of the {open_quotes}Reaktor-Sicherheitskommission{close_quotes} (RSK) in 1981 the requirements on the design have been changed with respect to the postulated leaks and breaks in the primary pressure boundary. The major change was a revision in the requirements for pipe whip protection. As a logical consequence of the {open_quotes}concept of basic safety{close_quotes} a guillotine type break or any other break type resulting in a large opening is not postulated any longer for the calculation of reaction and jet forces. As an upper limit for a leak an area of 0, 1 A (A = open cross section of the pipe) is postulated. This decision was based on a general assessment of the present PWR system design in Germany. Since then a number of piping systems have been requalified in the older nuclear power plants to comply with the break preclusion concept. Also a number of extensions of the concept have been developed to cover also leak-assumptions for branch pipes. Furthermore due considerations have been given to other aspects which could contribute to a leak development in the primary circuit, like vessel penetrations, manhole covers, flanges, etc. Now the break preclusion concept originally applied to the main piping has been developed into an integrated concept for the whole pressure boundary within the containment and will be applied also in the periodic safety review of present nuclear power plants.

  6. HANFORD DOUBLE SHELL TANK THERMAL AND SEISMIC PROJECT SENSITIVITY...

    Office of Scientific and Technical Information (OSTI)

    Title: HANFORD DOUBLE SHELL TANK THERMAL AND SEISMIC PROJECT SENSITIVITY OF DOUBLE SHELL ... the definition of the design ground motion or in the properties of the tank-waste system. ...

  7. WATER-LITHIUM BROMIDE DOUBLE-EFFECT ABSORPTION COOLING ANALYSIS

    Office of Scientific and Technical Information (OSTI)

    WATER-LITHIUM BROMIDE DOUBLE-EFFECT ABSORPTION COOLING ANALYSIS Gary C . V l i e t , ... i e n t simulation of the double-effect, water-1 ithium bromide absorption cooling ...

  8. Double photoionization of helium with synchrotron x-rays: Proceedings

    SciTech Connect (OSTI)

    Not Available

    1994-01-01

    This report contains papers on the following topics: Overview and comparison of photoionization with charged particle impact; The ratio of double to single ionization of helium: the relationship of photon and bare charged particle impact ionization; Double photoionization of helium at high energies; Compton scattering of photons from electrons bound in light elements; Electron ionization and the Compton effect in double ionization of helium; Elimination of two atomic electrons by a single energy photon; Double photoionization of helium at intermediate energies; Double Photoionization: Gauge Dependence, Coulomb Explosion; Single and Double Ionization by high energy photon impact; The effect of Compton Scattering on the double to single ionization ratio in helium; and Double ionization of He by photoionization and Compton scattering. These papers have been cataloged separately for the database.

  9. Sensitivity of CUORE to Neutrinoless Double-Beta Decay (Journal...

    Office of Scientific and Technical Information (OSTI)

    Sensitivity of CUORE to Neutrinoless Double-Beta Decay Citation Details In-Document Search Title: Sensitivity of CUORE to Neutrinoless Double-Beta Decay In this paper, we study the...

  10. Sensitivity of CUORE to Neutrinoless Double-Beta Decay (Journal...

    Office of Scientific and Technical Information (OSTI)

    Sensitivity of CUORE to Neutrinoless Double-Beta Decay Citation Details In-Document Search Title: Sensitivity of CUORE to Neutrinoless Double-Beta Decay You are accessing a...