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  1. Ormat Technologies Inc. Ormat Technologies Reports 2012 Fourth...

    Open Energy Info (EERE)

    Ormat Technologies Inc. Ormat Technologies Reports 2012 Fourth Quarter and Year End Results Jump to: navigation, search OpenEI Reference LibraryAdd to library Web Site: Ormat...

  2. Glass Buttes Exploration and Drilling: 2010 Geothermal Technologies Program Peer Review Presentation, Walsh, et al, Ormat

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Zemach, Ezra

    Glass Buttes Exploration and Drilling: 2010 Geothermal Technologies Program Peer Review Presentation, Walsh, et al, Ormat

  3. Glass Buttes Exploration and Drilling: 2010 Geothermal Technologies Program Peer Review Presentation, Walsh, et al, Ormat

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Zemach, Ezra

    2010-01-01

    Glass Buttes Exploration and Drilling: 2010 Geothermal Technologies Program Peer Review Presentation, Walsh, et al, Ormat

  4. Ormat Technologies Inc | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QAsource History ViewMayo, Maryland:NPIProtectio Program | OpenWisconsin: EnergyPark, Illinois:Orlovista,Ormat

  5. ORMAT NEVADA | Department of Energy

    Energy Savers [EERE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on DeliciousMathematicsEnergyInterested Parties -DepartmentAvailable forSite |n t787ORDER NO. 3357:ORMAT NEVADA ORMAT

  6. Ormat Technologies Inc. Ormat Technologies Reports 2012 Fourth Quarter and

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QAsource History ViewMayo, Maryland:NPIProtectio Program | OpenWisconsin: EnergyPark,Year End Results | Open Energy

  7. ORMAT NEVADA | Department of Energy

    Energy Savers [EERE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on DeliciousMathematicsEnergyInterested Parties -DepartmentAvailable forSite |n t787ORDER NO. 3357:ORMAT NEVADA

  8. Josh Nordquist Director Ormat Technologies, Inc. DOE's ...

    Broader source: Energy.gov (indexed) [DOE]

    homes) is being supplied to local grids in Canada and the US directly from the waste heat generated in the gas transmission process. This 100 MW is distributed amongst 19...

  9. Ormat Technologies Inc | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIX ECoop Inc Jump to:Newberg, Oregon:OGEProjects/DefinitionsOrchidx

  10. Ormat Technologies Inc | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIXsourceII JumpQuarterly Smart Grid DataInformationOpen Energy

  11. Ormat Technologies Inc. Ormat Technologies, Inc. Announces a Non-Cash

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIXsourceII JumpQuarterly Smart Grid DataInformationOpen EnergyPre-Tax Charge for

  12. EA-1849-S1: Phase II Facility- Ormat Tuscarora Geothermal Power Plant in Tuscarora, NV

    Broader source: Energy.gov [DOE]

    This Supplemental Environmental Assessment (SEA) will evaluate the potential impacts of the Phase II Facility of the Ormat Tuscarora Geothermal Power Plant.

  13. Assembling semiconductor nanocomposites using DNA replication technologies.

    SciTech Connect (OSTI)

    Heimer, Brandon W.; Crown, Kevin K.; Bachand, George David

    2005-11-01

    Deoxyribonucleic acid (DNA) molecules represent Nature's genetic database, encoding the information necessary for all cellular processes. From a materials engineering perspective, DNA represents a nanoscale scaffold with highly refined structure, stability across a wide range of environmental conditions, and the ability to interact with a range of biomolecules. The ability to mass-manufacture functionalized DNA strands with Angstrom-level resolution through DNA replication technology, however, has not been explored. The long-term goal of the work presented in this report is focused on exploiting DNA and in vitro DNA replication processes to mass-manufacture nanocomposite materials. The specific objectives of this project were to: (1) develop methods for replicating DNA strands that incorporate nucleotides with ''chemical handles'', and (2) demonstrate attachment of nanocrystal quantum dots (nQDs) to functionalized DNA strands. Polymerase chain reaction (PCR) and primer extension methodologies were used to successfully synthesize amine-, thiol-, and biotin-functionalized DNA molecules. Significant variability in the efficiency of modified nucleotide incorporation was observed, and attributed to the intrinsic properties of the modified nucleotides. Noncovalent attachment of streptavidin-coated nQDs to biotin-modified DNA synthesized using the primer extension method was observed by epifluorescence microscopy. Data regarding covalent attachment of nQDs to amine- and thiol-functionalized DNA was generally inconclusive; alternative characterization tools are necessary to fully evaluate these attachment methods. Full realization of this technology may facilitate new approaches to manufacturing materials at the nanoscale. In addition, composite nQD-DNA materials may serve as novel recognition elements in sensor devices, or be used as diagnostic tools for forensic analyses. This report summarizes the results obtained over the course of this 1-year project.

  14. Ormat Technologies Inc. North Brawley, California USA | Open Energy

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QAsource History ViewMayo, Maryland:NPIProtectio Program | OpenWisconsin: EnergyPark,

  15. BLM Fact Sheet- Ormat Technologies Salt Wells Geothermal Energy Project |

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIX ECoop IncIowaWisconsin: EnergyYorkColorado State Office Jump to: navigation,Open Energy

  16. EA-1849: Ormat Nevada Geothermal Projects in Northern NV | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE:FinancingPetroleum Based| Department8,DepartmentFinalinDepartmentSaginaw,Energy 9: Ormat Nevada

  17. Sub-Lithographic Patterning Technology for Nanowire Model Catalysts and DNA Label-Free Hybridization Detection

    E-Print Network [OSTI]

    Bokor, Jeffrey

    Sub-Lithographic Patterning Technology for Nanowire Model Catalysts and DNA Label were used as a mold in nanoimprint lithography and lift-off patterning of sub-30nm platinum nanowires a label-free tool for DNA hybridization detection based on measuring capacitance changes in the gap

  18. DNA isolation methods Many different methods and technologies are available for the

    E-Print Network [OSTI]

    Dever, Jennifer A.

    using this method, as the high levels of contamination often result in DNA degradation. 10 Genomic DNA using a detergent, and then mixed with phenol, chloroform, and isoamyl alcohol. The correct salt residual phenol and/or chloroform, which can inhibit enzyme reactions in downstream applications

  19. Vehicle Technologies Office Merit Review 2015: Fleet DNA Phase 1 Refinement & Phase 2 Implementation

    Broader source: Energy.gov [DOE]

    Presentation given by National Renewable Energy Laboratory at 2015 DOE Hydrogen and Fuel Cells Program and Vehicle Technologies Office Annual Merit Review and Peer Evaluation Meeting about Fleet...

  20. Microsoft Word - 338M_Geothermal_Project_Descriptions

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Location (City) Project Location (State) Description ORMAT Nevada, Inc. 4,911,330 Maui HI ORMAT Nevada will use a combination of technologies to locate fault zones within...

  1. Chimeric proteins for detection and quantitation of DNA mutations, DNA sequence variations, DNA damage and DNA mismatches

    DOE Patents [OSTI]

    McCutchen-Maloney, Sandra L. (Pleasanton, CA)

    2002-01-01

    Chimeric proteins having both DNA mutation binding activity and nuclease activity are synthesized by recombinant technology. The proteins are of the general formula A-L-B and B-L-A where A is a peptide having DNA mutation binding activity, L is a linker and B is a peptide having nuclease activity. The chimeric proteins are useful for detection and identification of DNA sequence variations including DNA mutations (including DNA damage and mismatches) by binding to the DNA mutation and cutting the DNA once the DNA mutation is detected.

  2. Nanotechnology with DNA DNA Nanodevices

    E-Print Network [OSTI]

    Ludwig-Maximilians-Universität, München

    Nanotechnology with DNA DNA Nanodevices Friedrich C. Simmel* and Wendy U. Dittmer A DNA actuator. Introduction.............285 2. Overview: DNA Nanotechnology.......285 3. Prototypes of Nanomechanical DNA overview of DNA nanotechnology as a whole is given. The most important properties of DNA molecules

  3. DNA Mixture DNA Mixture

    E-Print Network [OSTI]

    ;Introductions J.M. Butler ­ Wisconsin DNA Mixture Training May 12, 2009 http://www.cstl.nist.gov/biotech.M. Butler ­ Wisconsin DNA Mixture Training May 12, 2009 http://www.cstl.nist.gov/biotech textbook (now in its 2nd Edition) · STRBase website: http://www.cstl.nist.gov/biotech/strbase/ · Family

  4. Ormat Funding Corp | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QAsource History ViewMayo, Maryland:NPIProtectio Program | OpenWisconsin: EnergyPark, Illinois:Orlovista,

  5. Technolog

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    focuses on multi-scale, multiphysics approaches to understanding natural systems, "engineering the earth" with sensing and drilling technologies and characterizing geomaterials...

  6. The Future of Forensic DNA

    E-Print Network [OSTI]

    History and Mission · National Institute of Standards and Technology (NIST) was created in 1901The Future of Forensic DNA John M. Butler, PhD National Institute of Standards and Technology.S. Department of Commerce with a mission to develop and promote measurement, standards, and technology

  7. Technologies

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home RoomPreservationBio-Inspired Solar Fuel ProductionRecoverable15/2008Technologies Technologies

  8. Technology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home RoomPreservationBio-Inspired Solar FuelTechnology /newsroom/_assets/images/s-icon.png Technology

  9. Technologies

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power AdministrationRobust,Field-effectWorking With U.S. CoalMexico IndependentMatter and Technologies R&D

  10. Fleet DNA (Presentation)

    SciTech Connect (OSTI)

    Walkokwicz, K.; Duran, A.

    2014-06-01

    The Fleet DNA project objectives include capturing and quantifying drive cycle and technology variation for the multitude of medium- and heavy-duty vocations; providing a common data storage warehouse for medium- and heavy-duty vehicle fleet data across DOE activities and laboratories; and integrating existing DOE tools, models, and analyses to provide data-driven decision making capabilities. Fleet DNA advantages include: for Government - providing in-use data for standard drive cycle development, R&D, tech targets, and rule making; for OEMs - real-world usage datasets provide concrete examples of customer use profiles; for fleets - vocational datasets help illustrate how to maximize return on technology investments; for Funding Agencies - ways are revealed to optimize the impact of financial incentive offers; and for researchers -a data source is provided for modeling and simulation.

  11. Mitigating security issues in the evolving DNA synthesis industry

    E-Print Network [OSTI]

    Turlington, Ralph Donald, III

    2013-01-01

    DNA synthesis technologies are advancing at exponential rates, with production of ever longer, more complex, and less expensive sequences of double stranded DNA. This has fostered development of industrial scale design, ...

  12. NIJ-Funded Research in Forensic DNA

    E-Print Network [OSTI]

    Standards Funding from the FBI S&T Branch through NIST Information Access Division httpDNA) · Technology Evaluation and Development ­ Rapid multiplex PCR protocols* (PCR: 3 hr to FBI

  13. DNA Mixture Interpretation & Statistical Analysis

    E-Print Network [OSTI]

    of Standards and Technology Gaithersburg, Maryland John M. Butler CIB Forensic Science Center Training Seminar Mixture Workshop This workshop is for analysts, technical reviewers and technical leaders performing) National recommendations of the technical UK DNA working group on mixture interpretation for the NDNAD

  14. Recent advances in yeast molecular biology: recombinant DNA. [Lead abstract

    SciTech Connect (OSTI)

    Not Available

    1982-09-01

    Separate abstracts were prepared for the 25 papers presented at a workshop focusing on chromosomal structure, gene regulation, recombination, DNA repair, and cell type control, that have been obtained by experimental approaches incorporating the new technologies of yeast DNA transformation, molecular cloning, and DNA sequence analysis. (KRM)

  15. Storing data encoded DNA in living organisms

    DOE Patents [OSTI]

    Wong; Pak C. (Richland, WA), Wong; Kwong K. (Sugar Land, TX), Foote; Harlan P. (Richland, WA)

    2006-06-06

    Current technologies allow the generation of artificial DNA molecules and/or the ability to alter the DNA sequences of existing DNA molecules. With a careful coding scheme and arrangement, it is possible to encode important information as an artificial DNA strand and store it in a living host safely and permanently. This inventive technology can be used to identify origins and protect R&D investments. It can also be used in environmental research to track generations of organisms and observe the ecological impact of pollutants. Today, there are microorganisms that can survive under extreme conditions. As well, it is advantageous to consider multicellular organisms as hosts for stored information. These living organisms can provide as memory housing and protection for stored data or information. The present invention provides well for data storage in a living organism wherein at least one DNA sequence is encoded to represent data and incorporated into a living organism.

  16. Fleet DNA Project (Fact Sheet)

    SciTech Connect (OSTI)

    Not Available

    2012-10-01

    The Fleet DNA Project - designed by the U.S. Department of Energy's National Renewable Energy Laboratory (NREL) in partnership with Oak Ridge National Laboratory - aims to accelerate the evolution of advanced vehicle development and support the strategic deployment of market-ready technologies that reduce costs, fuel consumption, and emissions. At the heart of the Fleet DNA Project is a clearinghouse of medium- and heavy-duty commercial fleet transportation data for optimizing the design of advanced vehicle technologies or for selecting a given technology to invest in. An easy-to-access online database will help vehicle manufacturers and fleets understand the broad operational range for many of today's commercial vehicle vocations.

  17. Energy Department Finalizes Loan Guarantee for Ormat Geothermal...

    Office of Environmental Management (EM)

    largest solar generation facilities, three geothermal projects, the world's largest wind farm, and the nation's first new nuclear power plant in three decades. For more...

  18. EA-1849: Ormat Nevada Geothermal Projects in Northern NV | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    NV August 22, 2011 EA-1849: Final Environmental Assessment Tuscarora Geothermal Power Plant, Elko County, Nevada; Jersey Valley Geothermal Project, Pershing County, Nevada;...

  19. Energy Department Finalizes Loan Guarantee for Ormat Geothermal Project in

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of Natural GasAdjustmentsShirleyEnergy A plug-inPPLfor Innovative Solar Power Plant | Department of EnergyEnergy

  20. LPO5-002-Proj-Poster-CSP-Ormat

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious RankADVANCED MANUFACTURING OFFICE INDUSTRIALU.S. DepartmentJeanKeyLANLLG: Order4Tesla

  1. Advance the DNA computing 

    E-Print Network [OSTI]

    Qiu, Zhiquan Frank

    2004-09-30

    It has been previously shown that DNA computing can solve those problems currently intractable on even the fastest electronic computers. The algorithm design for DNA computing, however, is not straightforward. A strong background in both the DNA...

  2. Synthesis of DNA

    DOE Patents [OSTI]

    Mariella, Jr., Raymond P. (Danville, CA)

    2008-11-18

    A method of synthesizing a desired double-stranded DNA of a predetermined length and of a predetermined sequence. Preselected sequence segments that will complete the desired double-stranded DNA are determined. Preselected segment sequences of DNA that will be used to complete the desired double-stranded DNA are provided. The preselected segment sequences of DNA are assembled to produce the desired double-stranded DNA.

  3. DNA Engine Thermal Cycler

    E-Print Network [OSTI]

    Raizada, Manish N.

    ® Peltier Thermal Cycler PTC-0200 DNA Engine Cycler Operations Manual Version 4.0 #12;ii Tech Support: 1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .vi The DNA Engine® Peltier Thermal Cycler Introduction

  4. Context influences on TALE–DNA binding revealed by quantitative profiling

    E-Print Network [OSTI]

    Rogers, Julia M.

    Transcription activator-like effector (TALE) proteins recognize DNA using a seemingly simple DNA-binding code, which makes them attractive for use in genome engineering technologies that require precise targeting. Although ...

  5. DNA Topology: Fundamentals

    E-Print Network [OSTI]

    Mirkin, Sergei

    in Genome Functioning . Biological Role of Alternative DNA Structures Figure 1 A hypothetical circular DNA. 1ENCYCLOPEDIA OF LIFE SCIENCES / & 2001 Nature Publishing Group / www.els.net #12;topological

  6. The Fleet DNA Project (Fact Sheet), NREL (National Renewable...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of market-ready technologies that reduce costs, fuel consumption, and emissions. At the heart of the Fleet DNA Project is a clearinghouse of medium- and heavy-duty commercial fleet...

  7. International Standards in Forensic DNA and

    E-Print Network [OSTI]

    documentary (technical) standards physical (measurement) standards Certified reference material to aidInternational Standards in Forensic DNA and Recent Forensic Science Activities in the United States John M. Butler, Ph.D. National Institute of Standards and Technology NIST Fellow & Special Assistant

  8. Research Article DNA sequencing by microchip

    E-Print Network [OSTI]

    Barron, Annelise E.

    .1002/elps.200800389 1 Introduction The completion of the Human Genome Project [1, 2] has led to tremendous of the reasons for the early completion of the Human Genome Project was the technological advance- ment in DNA of personalized medicine based on the human genome cannot be fully realized until the cost of full human genome

  9. DNA Sequencing apparatus

    DOE Patents [OSTI]

    Tabor, Stanley (Cambridge, MA); Richardson, Charles C. (Chestnut Hill, MA)

    1992-01-01

    An automated DNA sequencing apparatus having a reactor for providing at least two series of DNA products formed from a single primer and a DNA strand, each DNA product of a series differing in molecular weight and having a chain terminating agent at one end; separating means for separating the DNA products to form a series bands, the intensity of substantially all nearby bands in a different series being different, band reading means for determining the position an This invention was made with government support including a grant from the U.S. Public Health Service, contract number AI-06045. The U.S. government has certain rights in the invention.

  10. Detection and quantitation of single nucleotide polymorphisms, DNA sequence variations, DNA mutations, DNA damage and DNA mismatches

    DOE Patents [OSTI]

    McCutchen-Maloney, Sandra L. (Pleasanton, CA)

    2002-01-01

    DNA mutation binding proteins alone and as chimeric proteins with nucleases are used with solid supports to detect DNA sequence variations, DNA mutations and single nucleotide polymorphisms. The solid supports may be flow cytometry beads, DNA chips, glass slides or DNA dips sticks. DNA molecules are coupled to solid supports to form DNA-support complexes. Labeled DNA is used with unlabeled DNA mutation binding proteins such at TthMutS to detect DNA sequence variations, DNA mutations and single nucleotide length polymorphisms by binding which gives an increase in signal. Unlabeled DNA is utilized with labeled chimeras to detect DNA sequence variations, DNA mutations and single nucleotide length polymorphisms by nuclease activity of the chimera which gives a decrease in signal.

  11. Emerging technologies

    SciTech Connect (OSTI)

    Lu, Shin-yee

    1993-03-01

    The mission of the Emerging Technologies thrust area at Lawrence Livermore National Laboratory is to help individuals establish technology areas that have national and commercial impact, and are outside the scope of the existing thrust areas. We continue to encourage innovative ideas that bring quality results to existing programs. We also take as our mission the encouragement of investment in new technology areas that are important to the economic competitiveness of this nation. In fiscal year 1992, we have focused on nine projects, summarized in this report: (1) Tire, Accident, Handling, and Roadway Safety; (2) EXTRANSYT: An Expert System for Advanced Traffic Management; (3) Odin: A High-Power, Underwater, Acoustic Transmitter for Surveillance Applications; (4) Passive Seismic Reservoir Monitoring: Signal Processing Innovations; (5) Paste Extrudable Explosive Aft Charge for Multi-Stage Munitions; (6) A Continuum Model for Reinforced Concrete at High Pressures and Strain Rates: Interim Report; (7) Benchmarking of the Criticality Evaluation Code COG; (8) Fast Algorithm for Large-Scale Consensus DNA Sequence Assembly; and (9) Using Electrical Heating to Enhance the Extraction of Volatile Organic Compounds from Soil.

  12. Introduction: DNA Electrophoresis Fralin Life Science

    E-Print Network [OSTI]

    Hopkins, William A.

    .................................... 12 Student Pre-Lab Activity: What is DNA? DNA extraction from strawberry ..... Teacher guide: DNA extraction from strawberry.................................. 14 Student guide: DNA extraction from strawberry.................................. 16

  13. Technology Assessment TECHNOLOGY ASSESSMENT

    E-Print Network [OSTI]

    Rock, Chris

    Technology Assessment 10/14/2004 1 TECHNOLOGY ASSESSMENT STRATEGIC PLAN MISSION STATEMENT Support the Mission of Texas Tech University and the TTU Information Technology Division by providing timely and relevant information and assistance in current and emerging technologies and their practical applications

  14. Bioscience Technology Bioscience Technology

    E-Print Network [OSTI]

    Vertes, Akos

    Bioscience Technology Bioscience Technology Advantage Business Media 100 Enterprise Drive Rockaway, co-director of George Washington University's Institute for Proteomics Technology and Applications-by-point. Manufacturers have stampeded to offer the new technology. Applied Biosystems got out in front in 2004 when

  15. Becky Hill Green Mountain DNA Conference LT-DNA Analysis

    E-Print Network [OSTI]

    Becky Hill ­ Green Mountain DNA Conference LT-DNA Analysis July 26, 2010 http of the Chief Medical Examiner, NYC Green Mountain DNA Conference Burlington, VT July 26, 2010 Low Template (LT generally aim for 0.5-2 ng 100 pg template 5 pg template #12;Becky Hill ­ Green Mountain DNA Conference LT

  16. Enhancing the DNA Patent Database

    SciTech Connect (OSTI)

    Walters, LeRoy B.

    2008-02-18

    Final Report on Award No. DE-FG0201ER63171 Principal Investigator: LeRoy B. Walters February 18, 2008 This project successfully completed its goal of surveying and reporting on the DNA patenting and licensing policies at 30 major U.S. academic institutions. The report of survey results was published in the January 2006 issue of Nature Biotechnology under the title “The Licensing of DNA Patents by US Academic Institutions: An Empirical Survey.” Lori Pressman was the lead author on this feature article. A PDF reprint of the article will be submitted to our Program Officer under separate cover. The project team has continued to update the DNA Patent Database on a weekly basis since the conclusion of the project. The database can be accessed at dnapatents.georgetown.edu. This database provides a valuable research tool for academic researchers, policymakers, and citizens. A report entitled Reaping the Benefits of Genomic and Proteomic Research: Intellectual Property Rights, Innovation, and Public Health was published in 2006 by the Committee on Intellectual Property Rights in Genomic and Protein Research and Innovation, Board on Science, Technology, and Economic Policy at the National Academies. The report was edited by Stephen A. Merrill and Anne-Marie Mazza. This report employed and then adapted the methodology developed by our research project and quoted our findings at several points. (The full report can be viewed online at the following URL: http://www.nap.edu/openbook.php?record_id=11487&page=R1). My colleagues and I are grateful for the research support of the ELSI program at the U.S. Department of Energy.

  17. A Rapid Micro Polymerase Chain Reaction System (GenSpector Micro PCR) for Hepatitis B Virus DNA Detection

    E-Print Network [OSTI]

    Oh, Kwang W.

    A Rapid Micro Polymerase Chain Reaction System (GenSpector® Micro PCR) for Hepatitis B Virus DNA reaction) system (GenSpector® Micro PCR) for the application of Hepatitis B virus (HBV) DNA detection of Hepatitis B virus (HBV) DNA detection [1]. Silicon micromachining technology has been utilized

  18. Multiplex analysis of DNA

    DOE Patents [OSTI]

    Church, George M. (Boston, MA); Kieffer-Higgins, Stephen (Dorchester, MA)

    1992-01-01

    This invention features vectors and a method for sequencing DNA. The method includes the steps of: a) ligating the DNA into a vector comprising a tag sequence, the tag sequence includes at least 15 bases, wherein the tag sequence will not hybridize to the DNA under stringent hybridization conditions and is unique in the vector, to form a hybrid vector, b) treating the hybrid vector in a plurality of vessels to produce fragments comprising the tag sequence, wherein the fragments differ in length and terminate at a fixed known base or bases, wherein the fixed known base or bases differs in each vessel, c) separating the fragments from each vessel according to their size, d) hybridizing the fragments with an oligonucleotide able to hybridize specifically with the tag sequence, and e) detecting the pattern of hybridization of the tag sequence, wherein the pattern reflects the nucleotide sequence of the DNA.

  19. Shear Unzipping of DNA

    E-Print Network [OSTI]

    Buddhapriya Chakrabarti; David R. Nelson

    2009-04-09

    We study theoretically the mechanical failure of a simple model of double stranded DNA under an applied shear. Starting from a more microscopic Hamiltonian that describes a sheared DNA, we arrive at a nonlinear generalization of a ladder model of shear unzipping proposed earlier by deGennes [deGennes P. G. C. R. Acad. Sci., Ser. IV; Phys., Astrophys. 2001, 1505]. Using this model and a combination of analytical and numerical methods, we study the DNA "unzipping" transition when the shearing force exceeds a critical threshold at zero temperature. We also explore the effects of sequence heterogeneity and finite temperature and discuss possible applications to determine the strength of colloidal nanoparticle assemblies functionalized by DNA.

  20. National Account Energy Alliance Final Report for the Basin Electric Project at Northern Border Pipeline Company's Compressor Station #7, North Dakota

    SciTech Connect (OSTI)

    Sweetzer, Richard [Exergy Partners Corp.; Leslie, Neil [Gas Technology Institute

    2008-02-01

    A field research test and verification project was conducted at the recovered energy generation plant at Northern Border Pipeline Company Compressor Station #7 (CS#7) near St. Anthony. Recovered energy generation plant equipment was supplied and installed by ORMAT Technologies, Inc. Basin Electric is purchasing the electricity under a purchase power agreement with an ORMAT subsidiary, which owns and operates the plant.

  1. DNA banking and DNA databanking: Legal, ethical, and public policy issues. Progress report, [April 1, 1993--March 31, 1994

    SciTech Connect (OSTI)

    Reilly, P.R.; McEwen, J.E.; Small, D.

    1994-02-18

    The purpose of the grant was to provide support to enable us to: (1) perform legal and empirical research and critically analyze DNA banking and DNA databanking as those activities are conducted by state forensic laboratories, the military, academic researchers, and commercial enterprises; and (2) develop a broadcast quality educational videotape for viewing by the general public about DNA technology and the privacy and related issues that it raises. The grant thus has both a research and analysis component and a public education component. This report outlines the work completed since the inception of the project and describes the activities still in progress.

  2. Coarse-graining DNA for simulations of DNA nanotechnology

    E-Print Network [OSTI]

    Jonathan P. K. Doye; Thomas E. Ouldridge; Ard A. Louis; Flavio Romano; Petr Sulc; Christian Matek; Benedict E. K. Snodin; Lorenzo Rovigatti; John S. Schreck; Ryan M. Harrison; William P. J. Smith

    2013-08-18

    To simulate long time and length scale processes involving DNA it is necessary to use a coarse-grained description. Here we provide an overview of different approaches to such coarse graining, focussing on those at the nucleotide level that allow the self-assembly processes associated with DNA nanotechnology to be studied. OxDNA, our recently-developed coarse-grained DNA model, is particularly suited to this task, and has opened up this field to systematic study by simulations. We illustrate some of the range of DNA nanotechnology systems to which the model is being applied, as well as the insights it can provide into fundamental biophysical properties of DNA.

  3. Coarse-graining DNA for simulations of DNA nanotechnology

    E-Print Network [OSTI]

    Doye, Jonathan P K; Louis, Ard A; Romano, Flavio; Sulc, Petr; Matek, Christian; Snodin, Benedict E K; Rovigatti, Lorenzo; Schreck, John S; Harrison, Ryan M; Smith, William P J

    2013-01-01

    To simulate long time and length scale processes involving DNA it is necessary to use a coarse-grained description. Here we provide an overview of different approaches to such coarse graining, focussing on those at the nucleotide level that allow the self-assembly processes associated with DNA nanotechnology to be studied. OxDNA, our recently-developed coarse-grained DNA model, is particularly suited to this task, and has opened up this field to systematic study by simulations. We illustrate some of the range of DNA nanotechnology systems to which the model is being applied, as well as the insights it can provide into fundamental biophysical properties of DNA.

  4. Faience Technology

    E-Print Network [OSTI]

    Nicholson, Paul

    2009-01-01

    by Joanne Hodges. Faience Technology, Nicholson, UEE 2009Egyptian materials and technology, ed. Paul T. Nicholson,Nicholson, 2009, Faience Technology. UEE. Full Citation:

  5. DNA polymerase having modified nucleotide binding site for DNA sequencing

    DOE Patents [OSTI]

    Tabor, S.; Richardson, C.

    1997-03-25

    A modified gene encoding a modified DNA polymerase is disclosed. The modified polymerase incorporates dideoxynucleotides at least 20-fold better compared to the corresponding deoxynucleotides as compared with the corresponding naturally-occurring DNA polymerase. 6 figs.

  6. Searching for DNA Lesions: Structural Evidence for Lower- and Higher-Affinity DNA Binding Conformations of Human Alkyladenine DNA Glycosylase

    E-Print Network [OSTI]

    Drennan, Catherine L.

    To efficiently repair DNA, human alkyladenine DNA glycosylase (AAG) must search the million-fold excess of unmodified DNA bases to find a handful of DNA lesions. Such a search can be facilitated by the ability of glycosylases, ...

  7. Molecular display of synthetic oligonucleotide libraries and their analysis with high throughput DNA sequencing

    E-Print Network [OSTI]

    Larman, Harry Benjamin

    2012-01-01

    High throughput methods in molecular biology have changed the landscape of biomedical research. In particular, advances in massively parallel DNA sequencing and synthesis technologies are defining our genomes and the ...

  8. DNA nanoconjugates Labeling TiO2 Nanoparticles with Dyes for Optical

    E-Print Network [OSTI]

    Brown, Eric

    signal as mapped by XFM. This strongly implies the intracellular integrity of the TiO2­DNA nanoconjugates Indian Institute of Technology Bombay Powai Mumbai 400076 (India) Prof. V. Dravid Department of Material

  9. DNA Structural Nanotechnology Duke University

    E-Print Network [OSTI]

    Reif, John H.

    DNA Structural Nanotechnology John Reif Duke University Graduate Students: Harish Chandran&Caltech Tube Lattices #12;Ned Seeman New York University, USA Ned Seeman: Father of DNA Nanotechnology His Initial Ideas & Motivation for DNA Nanotechnology #12;Cube Chen & Seeman, Nature350:631 (1991) Truncated

  10. Estimating Dataset Size Requirements for Classifying DNA Microarray Data

    E-Print Network [OSTI]

    Poggio, Tomaso

    + . + Whitehead Institute / Massachusetts Institute of Technology Center for Genome Research, Cambridge, MA 02139 approaches based on machine learning algorithms applied to DNA microarray data have been shown to have to extrapolate error statistics for larger datasets. Power calculations [Adcock 1997] are a standard approach

  11. DNA waves and water

    E-Print Network [OSTI]

    L. Montagnier; J. Aissa; E. Del Giudice; C. Lavallee; A. Tedeschi; G. Vitiello

    2010-12-23

    Some bacterial and viral DNA sequences have been found to induce low frequency electromagnetic waves in high aqueous dilutions. This phenomenon appears to be triggered by the ambient electromagnetic background of very low frequency. We discuss this phenomenon in the framework of quantum field theory. A scheme able to account for the observations is proposed. The reported phenomenon could allow to develop highly sensitive detection systems for chronic bacterial and viral infections.

  12. DNA | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIX E LISTStar EnergyLawler,CoalConcordiaConsumerLEDSEnergyDMS Company Ltd Jump to:DNA Jump

  13. Exploration Technologies - Technology Needs Assessment

    SciTech Connect (OSTI)

    Greene, Amanda I.; Thorsteinsson, Hildigunnur; Reinhardt, Tim; Solomon, Samantha; James, Mallory

    2011-06-01

    This assessment is a critical component of ongoing technology roadmapping efforts, and will be used to guide the Geothermal Technology Program's research and development.

  14. Method for assaying clustered DNA damages

    DOE Patents [OSTI]

    Sutherland, Betsy M.

    2004-09-07

    Disclosed is a method for detecting and quantifying clustered damages in DNA. In this method, a first aliquot of the DNA to be tested for clustered damages with one or more lesion-specific cleaving reagents under conditions appropriate for cleavage of the DNA to produce single-strand nicks in the DNA at sites of damage lesions. The number average molecular length (Ln) of double stranded DNA is then quantitatively determined for the treated DNA. The number average molecular length (Ln) of double stranded DNA is also quantitatively determined for a second, untreated aliquot of the DNA. The frequency of clustered damages (.PHI..sub.c) in the DNA is then calculated.

  15. Thermally Activated Technologies Technology Roadmap, May 2003...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Thermally Activated Technologies Technology Roadmap, May 2003 Thermally Activated Technologies Technology Roadmap, May 2003 The purpose of this Technology Roadmap is to outline a...

  16. Normalized cDNA libraries

    DOE Patents [OSTI]

    Soares, M.B.; Efstratiadis, A.

    1997-06-10

    This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3{prime} noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library. 4 figs.

  17. Normalized cDNA libraries

    DOE Patents [OSTI]

    Soares, Marcelo B. (New York, NY); Efstratiadis, Argiris (Englewood, NJ)

    1997-01-01

    This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3' noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library.

  18. Sequence independent amplification of DNA

    DOE Patents [OSTI]

    Bohlander, S.K.

    1998-03-24

    The present invention is a rapid sequence-independent amplification procedure (SIA). Even minute amounts of DNA from various sources can be amplified independent of any sequence requirements of the DNA or any a priori knowledge of any sequence characteristics of the DNA to be amplified. This method allows, for example, the sequence independent amplification of microdissected chromosomal material and the reliable construction of high quality fluorescent in situ hybridization (FISH) probes from YACs or from other sources. These probes can be used to localize YACs on metaphase chromosomes but also--with high efficiency--in interphase nuclei. 25 figs.

  19. ORNI 9, LLC, AND ORMAT NEVADA INC. APPLICATIONS FOR PERMIT TO DRILL

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE:Financing Tool Fits the BillDepartmentSitesUMTRCA3 ANNUAL REPORT OHAOPAM8, 2014JulyORNI 9, LLC, AND

  20. Ormat Becomes Sole Owner of the Mammoth Complex in Mammoth Lakes,

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIXsourceII JumpQuarterly Smart Grid DataInformationOpen Energy

  1. Ormat's North Brawley plant with 17MW short of its 50MW potential | Open

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIXsourceII JumpQuarterly Smart Grid DataInformationOpen EnergyPre-Tax Charge forEnergy

  2. EA-1849-S1: Phase II Facility - Ormat Tuscarora Geothermal Power Plant in

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE:FinancingPetroleum Based| Department8,DepartmentFinalinDepartmentSaginaw, MIEnergyFinding of

  3. Evolutionary theory, web-search technology combine for DNA analysis

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home Room NewsInformation Current HABFES OctoberEvan Racah Evan Racah 1517546Has Evidence ofofSequedex:

  4. Topics in Forensic DNA Analysis &

    E-Print Network [OSTI]

    chemistry from the University of Virginia. His dissertation research, which was conducted at the FBI Academy guest of the FBI's Scientific Working Group on DNA Analysis Methods (SWGDAM) and a member

  5. High Impact Technology Catalyst: Technology Deployment Strategies...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    High Impact Technology Catalyst: Technology Deployment Strategies High Impact Technology Catalyst: Technology Deployment Strategies The Energy Department released the High Impact...

  6. Vehicle Technologies Office: 2014 Electric Drive Technologies...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Vehicle Technologies Office: 2014 Electric Drive Technologies Annual Progress Report Vehicle Technologies Office: 2014 Electric Drive Technologies Annual Progress Report The...

  7. Technology '90

    SciTech Connect (OSTI)

    Not Available

    1991-01-01

    The US Department of Energy (DOE) laboratories have a long history of excellence in performing research and development in a number of areas, including the basic sciences, applied-energy technology, and weapons-related technology. Although technology transfer has always been an element of DOE and laboratory activities, it has received increasing emphasis in recent years as US industrial competitiveness has eroded and efforts have increased to better utilize the research and development resources the laboratories provide. This document, Technology '90, is the latest in a series that is intended to communicate some of the many opportunities available for US industry and universities to work with the DOE and its laboratories in the vital activity of improving technology transfer to meet national needs. Technology '90 is divided into three sections: Overview, Technologies, and Laboratories. The Overview section describes the activities and accomplishments of the DOE research and development program offices. The Technologies section provides descriptions of new technologies developed at the DOE laboratories. The Laboratories section presents information on the missions, programs, and facilities of each laboratory, along with a name and telephone number of a technology transfer contact for additional information. Separate papers were prepared for appropriate sections of this report.

  8. Technology Assessment

    Office of Environmental Management (EM)

    capabilities that are energy efficient, low environmental impact 72 and lower cost and that are employed to manufacture technologies and products for clean energy 73...

  9. Delineating Rearrangements in Single Yeast Artificial Chromosomes by Quantitative DNA Fiber Mapping

    SciTech Connect (OSTI)

    Weier, Heinz-Ulrich G.; Greulich-Bode, Karin M.; Wu, Jenny; Duell, Thomas

    2009-09-18

    Cloning of large chunks of human genomic DNA in recombinant systems such as yeast or bacterial artificial chromosomes has greatly facilitated the construction of physical maps, the positional cloning of disease genes or the preparation of patient-specific DNA probes for diagnostic purposes. For this process to work efficiently, the DNA cloning process and subsequent clone propagation need to maintain stable inserts that are neither deleted nor otherwise rearranged. Some regions of the human genome; however, appear to have a higher propensity than others to rearrange in any host system. Thus, techniques to detect and accurately characterize such rearrangements need to be developed. We developed a technique termed 'Quantitative DNA Fiber Mapping (QDFM)' that allows accurate tagging of sequence elements of interest with near kilobase accuracy and optimized it for delineation of rearrangements in recombinant DNA clones. This paper demonstrates the power of this microscopic approach by investigating YAC rearrangements. In our examples, high-resolution physical maps for regions within the immunoglobulin lambda variant gene cluster were constructed for three different YAC clones carrying deletions of 95 kb and more. Rearrangements within YACs could be demonstrated unambiguously by pairwise mapping of cosmids along YAC DNA molecules. When coverage by YAC clones was not available, distances between cosmid clones were estimated by hybridization of cosmids onto DNA fibers prepared from human genomic DNA. In addition, the QDFM technology provides essential information about clone stability facilitating closure of the maps of the human genome as well as those of model organisms.

  10. Characterization of nanoparticle-DNA conjugate and control of DNA conformation on particle surface

    E-Print Network [OSTI]

    Park, Sunho, 1976-

    2009-01-01

    Nano-science has exploited the hybridization and de-hybridization phenomena of DNA which are one of its fundamental functions. In particular, conjugates of gold nanoparticles and DNA (Au NP-DNA) have been extensively ...

  11. Regulation of DNA damage tolerance : studies of the translesion synthesis DNA ploymerase eta in Saccharomyces cerevisiae

    E-Print Network [OSTI]

    Woodruff, Rachel Van Etten

    2008-01-01

    All organisms must control the effects of DNA damage to protect the integrity of their genomes. In addition to DNA repair, this requires DNA damage tolerance pathways, which allow the continuation of essential processes ...

  12. Technology Commercialization Showcase 2008 Vehicle Technologies Program

    SciTech Connect (OSTI)

    Davis, Patrick B.

    2009-06-19

    Presentation illustrating various technology commercialization opportunities and unexploited investment gaps for the Vehicle Technologies Program.

  13. Magnetic tweezers to studyMagnetic tweezers to studyMagnetic tweezers to studyMagnetic tweezers to study DNA motorsDNA motorsDNA motorsDNA motors

    E-Print Network [OSTI]

    Ritort, Felix

    to study DNA motorsDNA motorsDNA motorsDNA motors MariaMariaMariaMaria MañosasMañosasMañosasMañosas Ritort) · Applications: 1. Tracking DNA motors: (i) Helicases (ii) Annealing motor 2. Studying a multiprotein system: DNA hexamers (Dong et al, JBC 1995) Tracking DNA motors: (i) Helicases #12;Passive: helicase behaves

  14. Thermally activated technologies: Technology Roadmap

    SciTech Connect (OSTI)

    None, None

    2003-05-01

    The purpose of this Technology Roadmap is to outline a set of actions for government and industry to develop thermally activated technologies for converting America’s wasted heat resources into a reservoir of pollution-free energy for electric power, heating, cooling, refrigeration, and humidity control. Fuel flexibility is important. The actions also cover thermally activated technologies that use fossil fuels, biomass, and ultimately hydrogen, along with waste heat.

  15. sustainable technologies

    E-Print Network [OSTI]

    Zhang, Junshan

    : · realize continuous improvements in performance (efficiency), cost and manufacturability of PV technologies, transformative PV technologies that circumvent cost/performance trade-offs and maintain compatibility with P the growing demand for energy. Photovoltaics (PV) leverages one of the 20th century's greatest scientific

  16. The Structure of DNA within Cationic Lipid/DNA Complexes

    E-Print Network [OSTI]

    Braun, Chad S.; Jas, Gouri S.; Choosakoonriang, Sirirat; Koe, Gary S.; Smith, Janet G.; Middaugh, C. Russell

    2003-02-01

    hexagonal phase of cationic liposome-DNA complexes related to DNA release and delivery. Science. 281:78–81. Koppel, D. E. 1972. Analysis of macromolecular polydispersity in inten- sity correlation spectroscopy: the method of cumulants. J. Chem. Phys. 57:4814..., DOTAP, 1,2-dioleoyl-sn-glycero-3-phosphatidyletha- nolamine, and cholesterol were purchased from Avanti Polar Lipids (Alabaster, AL). Poly(dG) Æ poly(dC) (4 kbp), poly(dA) Æ poly(dT) (;229 bp), poly(dGdC) Æ poly(dGdC) (724 bp), and poly(dAdT) Æ poly(d...

  17. DNA repair decline during mouse spermiogenesis results in the accumulation of heritable DNA damage

    E-Print Network [OSTI]

    Marchetti, Francesco

    2008-01-01

    male germ cells handle DNA damage? Toxicol. Appl. Pharmacol.strand breaks and DNA base damage at different cellularrelationship to genetic damage, Mutat. Res. 216 (1989) 221-

  18. DNA Repair Decline During Mouse Spermiogenesis Results in the Accumulation of Heritable DNA Damage

    E-Print Network [OSTI]

    Marchetti, Francesco

    2008-01-01

    male germ cells handle DNA damage? Toxicol. Appl. Pharmacol.strand breaks and DNA base damage at different cellularrelationship to genetic damage, Mutat. Res. 216 (1989) 221-

  19. INTERSTATE WASTE TECHNOLOGIES THERMOSELECT TECHNOLOGY

    E-Print Network [OSTI]

    Columbia University

    1 INTERSTATE WASTE TECHNOLOGIES THERMOSELECT TECHNOLOGY AN OVERVIEW Presented to the DELAWARE SOLID WASTE MANAGEMENT TECHNICAL WORKING GROUP January 10, 2006 #12;2 INTERSTATE WASTE MANAGEMENT ALLIANCE and maintenance (30 years) ­ Will guarantee performance and Operation and Maintenance ­ Serves solid waste

  20. Technology Validation

    Broader source: Energy.gov [DOE]

    To reduce solar technology risks, DOE and its partners evaluate the performance and reliability of novel photovoltaic (PV) hardware and systems through laboratory and field testing. The focus of...

  1. Bioconjugated Nanoparticles for DNA Protection from Cleavage Xiao-xiao He, Kemin Wang,* Weihong Tan, Bin Liu, Xia Lin, Chunmei He, Du Li,

    E-Print Network [OSTI]

    Tan, Weihong

    in water-in-oil microemulsion. Briefly, a well- distributed mixture of the two silanes (with volume ratio 1/Biosensing and Chemometrics, College of Chemistry & Chemical Engineering, Institute of Biological Technology, Hunan Uni and 4, plasmid DNA moved in the electric field, and amino-modified silica nanopar- ticles-plasmid DNA

  2. The Initiation of Bacterial DNA Replication

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    for this system. Instead, DnaA forms an open right-handed helix. In addition, the architecture indicates that this AAA+ superhelix will wrap coils of the DNA around its exterior,...

  3. Micropatterned cell arrays for detecting DNA damage

    E-Print Network [OSTI]

    Mittal, Sukant

    2008-01-01

    Numerous agents are capable of interacting with DNA and damaging it. Permanent changes in the DNA structure can be both mutagenic and cytotoxic; therefore, methods to measure the susceptibility of cells to mutations are ...

  4. Towards Privacy Preserving of Forensic DNA Databases 

    E-Print Network [OSTI]

    Liu, Sanmin

    2012-02-14

    Protecting privacy of individuals is critical for forensic genetics. In a kinship/identity testing, related DNA profiles between user's query and the DNA database need to be extracted. However, unrelated profiles cannot be revealed to each other...

  5. Technology Roadmap Analysis 2013: Assessing Automotive Technology...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Technology Roadmap Analysis 2013: Assessing Automotive Technology R&D Relevant to DOE Power Electronics Cost Targets Technology Roadmap Analysis 2013: Assessing Automotive...

  6. Chromosome specific repetitive DNA sequences

    DOE Patents [OSTI]

    Moyzis, Robert K. (Los Alamos, NM); Meyne, Julianne (Los Alamos, NM)

    1991-01-01

    A method is provided for determining specific nucleotide sequences useful in forming a probe which can identify specific chromosomes, preferably through in situ hybridization within the cell itself. In one embodiment, chromosome preferential nucleotide sequences are first determined from a library of recombinant DNA clones having families of repetitive sequences. Library clones are identified with a low homology with a sequence of repetitive DNA families to which the first clones respectively belong and variant sequences are then identified by selecting clones having a pattern of hybridization with genomic DNA dissimilar to the hybridization pattern shown by the respective families. In another embodiment, variant sequences are selected from a sequence of a known repetitive DNA family. The selected variant sequence is classified as chromosome specific, chromosome preferential, or chromosome nonspecific. Sequences which are classified as chromosome preferential are further sequenced and regions are identified having a low homology with other regions of the chromosome preferential sequence or with known sequences of other family me This invention is the result of a contract with the Department of Energy (Contract No. W-7405-ENG-36).

  7. Origami DNA model Mountain fold

    E-Print Network [OSTI]

    Csürös, Miklós

    Origami DNA model Mountain fold Solid lines are "mountains" and are to be folded away from you with the peak pointing towards you. 1. Fold all solid lines going lengthwise down the page into "mountain folds fold 2. Fold all dashed lines going lengthwise down the page into "valley folds". Mountain folds along

  8. The Future of Forensic DNA

    E-Print Network [OSTI]

    ;Checks and Controls on DNA Results Community FBI Quality Assurance Standards (and interlaboratory studies Washington D.C. Dulles Airport Reagan National Airport BWI Airport NIST FBI Lab Baltimore, MD Richmond, VA Materials (SRMs) Helps meet FBI QAS and ISO 17025 requirements Traceable standards to ensure accurate

  9. DNA sequencing protocols BN Danforth

    E-Print Network [OSTI]

    Danforth, Bryan Nicholas

    in the degradation of DNA. (7) Spin the tubes down at the end to remove condensation from tops of tubes. B. Extraction and RNA digestion. NOTE: We now skip the Rnase step. You will need: Phenol and gently invert several times. This step removes the phenol from the previous extraction. Spin

  10. Building Technologies Office Overview

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Roland Risser Director, Building Technologies Office Building Technologies Office Energy Efficiency Starts Here. 2 Building Technologies Office Integrated Approach: Improving...

  11. Biological Physics of DNA Typeset by FoilTEX 1

    E-Print Network [OSTI]

    Potsdam, Universität

    fragments to ssDNA Labelling: eg radioact probe fragm & Xray film http;Packaging of DNA in bacteria 11 #12;DNA melting 12 #12;Polymerase chain reaction Heating dsDNA sample 2

  12. DNA extraction techniques for DNA barcoding of minute gall-inhabiting wasps

    E-Print Network [OSTI]

    extraction methods were compared to determine their efficacy in isolating DNA. Success of each methodDNA extraction techniques for DNA barcoding of minute gall-inhabiting wasps GUDRUN DITTRICH, South Africa Abstract DNA extraction from minute hymenopterans and their larvae is difficult

  13. DNA Word Design Strategy for Creating Sets of Non-interacting Oligonucleotides for DNA Microarrays

    E-Print Network [OSTI]

    DNA Word Design Strategy for Creating Sets of Non-interacting Oligonucleotides for DNA Microarrays mismatches with the complements of all the other members in the set. These "DNA word" sets are denoted as nbm. To achieve good discrimination between each DNA word in each set generated using the template-map strategy

  14. Probe and method for DNA detection

    DOE Patents [OSTI]

    Yeh, Hsin-Chih; Werner, James Henry; Sharma, Jaswinder Kumar; Martinez, Jennifer Suzanne

    2013-07-02

    A hybridization probe containing two linear strands of DNA lights up upon hybridization to a target DNA using silver nanoclusters that have been templated onto one of the DNA strands. Hybridization induces proximity between the nanoclusters on one strand and an overhang on the other strand, which results in enhanced fluorescence emission from the nanoclusters.

  15. MATERIALS AND METHODS 1) DNA extraction

    E-Print Network [OSTI]

    Collins, Gary S.

    MATERIALS AND METHODS 1) DNA extraction · DNA was extracted from the ileo-cecal nodes of 475 Holstein cows from two herds using the Qiagen DNA extraction kit (Valencia, CA). 2) Map detection · Map was extracted from ileo-cecal nodes using Ambion's MagMAX Total Nucleic Acid Isolation kit (Austin, TX

  16. Binding of undamaged double stranded DNA to vaccinia virus uracil-DNA glycosylase

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Schormann, Norbert; Banerjee, Surajit; Ricciardi, Robert; Chattopadhyay, Debasish

    2015-06-02

    Background: Uracil-DNA glycosylases are evolutionarily conserved DNA repair enzymes. However, vaccinia virus uracil-DNA glycosylase (known as D4), also serves as an intrinsic and essential component of the processive DNA polymerase complex during DNA replication. In this complex D4 binds to a unique poxvirus specific protein A20 which tethers it to the DNA polymerase. At the replication fork the DNA scanning and repair function of D4 is coupled with DNA replication. So far, DNA-binding to D4 has not been structurally characterized. Results: This manuscript describes the first structure of a DNA-complex of a uracil-DNA glycosylase from the poxvirus family. This alsomore »represents the first structure of a uracil DNA glycosylase in complex with an undamaged DNA. In the asymmetric unit two D4 subunits bind simultaneously to complementary strands of the DNA double helix. Each D4 subunit interacts mainly with the central region of one strand. DNA binds to the opposite side of the A20-binding surface on D4. In comparison of the present structure with the structure of uracil-containing DNA-bound human uracil-DNA glycosylase suggests that for DNA binding and uracil removal D4 employs a unique set of residues and motifs that are highly conserved within the poxvirus family but different in other organisms. Conclusion: The first structure of D4 bound to a truly non-specific undamaged double-stranded DNA suggests that initial binding of DNA may involve multiple non-specific interactions between the protein and the phosphate backbone.« less

  17. Science & Technology Review September 2009

    SciTech Connect (OSTI)

    Bearinger, J P

    2009-07-24

    This month's issue has the following articles: (1) Remembering the Laboratory's First Director - Commentary by Harold Brown; (2) Herbert F. York (1921-2009): A Life of Firsts, an Ambassador for Peace - The Laboratory's first director, who died on May 19, 2009, used his expertise in science and technology to advance arms control and prevent nuclear war; (3) Searching for Life in Extreme Environments - DNA will help researchers discover new marine species and prepare to search for life on other planets; (4) Energy Goes with the Flow - Lawrence Livermore is one of the few organizations that distills the big picture about energy resources and use into a concise diagram; and (5) The Radiant Side of Sound - An experimental method that converts sound waves into light may lead to new technologies for scientific and industrial applications.

  18. Microfluidic DNA sample preparation method and device

    DOE Patents [OSTI]

    Krulevitch, Peter A. (Pleasanton, CA); Miles, Robin R. (Danville, CA); Wang, Xiao-Bo (San Diego, CA); Mariella, Raymond P. (Danville, CA); Gascoyne, Peter R. C. (Bellaire, TX); Balch, Joseph W. (Livermore, CA)

    2002-01-01

    Manipulation of DNA molecules in solution has become an essential aspect of genetic analyses used for biomedical assays, the identification of hazardous bacterial agents, and in decoding the human genome. Currently, most of the steps involved in preparing a DNA sample for analysis are performed manually and are time, labor, and equipment intensive. These steps include extraction of the DNA from spores or cells, separation of the DNA from other particles and molecules in the solution (e.g. dust, smoke, cell/spore debris, and proteins), and separation of the DNA itself into strands of specific lengths. Dielectrophoresis (DEP), a phenomenon whereby polarizable particles move in response to a gradient in electric field, can be used to manipulate and separate DNA in an automated fashion, considerably reducing the time and expense involved in DNA analyses, as well as allowing for the miniaturization of DNA analysis instruments. These applications include direct transport of DNA, trapping of DNA to allow for its separation from other particles or molecules in the solution, and the separation of DNA into strands of varying lengths.

  19. Contributing Data to the Fleet DNA Project (Brochure)

    SciTech Connect (OSTI)

    Not Available

    2014-09-01

    The Fleet DNA clearinghouse of commercial fleet transportation data helps vehicle manufacturers and developers optimize vehicle designs and helps fleet managers choose advanced technologies for their fleets. This online tool - available at www.nrel.gov/fleetdna - provides data summaries and visualizations similar to real-world 'genetics' for medium- and heavy-duty commercial fleet vehicles operating within a variety of vocations. To contribute your fleet data, please contact Adam Duran of the U.S. Department of Energy's National Renewable Energy Laboratory (NREL) at adam.duran@nrel.gov or 303-275-4586.

  20. Unravelling Microbial Communities with DNA-Microarrays: Challengesand Future Directions.

    SciTech Connect (OSTI)

    Wagner, Michael; Smidt, Hauke; Loy, Alexander; Zhou, Jizhong

    2007-03-08

    High-throughput technologies are urgently needed formonitoring the formidable biodiversity and functional capabilities ofmicroorganisms in the environment. Ten years ago, DNA microarrays,miniaturized platforms for highly parallel hybridization reactions, foundtheir way into environmental microbiology and raised great expectationsamong researchers in the field. In this article, we briefly summarize thestate-of-the-art of microarray approaches in microbial ecology researchand discuss in more detail crucial problems and promising solutions.Finally, we outline scenarios for an innovative combination ofmicroarrays with other molecular tools for structure-function analysis ofcomplex microbial communities.

  1. ANALYTICAL SCIENCE & TECHNOLOGY

    E-Print Network [OSTI]

    Kemp, Brian M.

    found that a phenol/chloroform extraction, followed by ultrafiltration, and cleanup by via the QIAquick research on exogenous contamination,1 problems with DNA degradation and damage,2 and the possible co that DNA extracted from bone yields minimal amounts of degraded human DNA, mixed with high amounts

  2. Electromagnetic Signals from Bacterial DNA

    E-Print Network [OSTI]

    A. Widom; J. Swain; Y. N. Srivastava; S. Sivasubramanian

    2012-02-09

    Chemical reactions can be induced at a distance due to the propagation of electromagnetic signals during intermediate chemical stages. Although is is well known at optical frequencies, e.g. photosynthetic reactions, electromagnetic signals hold true for muck lower frequencies. In E. coli bacteria such electromagnetic signals can be generated by electric transitions between energy levels describing electrons moving around DNA loops. The electromagnetic signals between different bacteria within a community is a "wireless" version of intercellular communication found in bacterial communities connected by "nanowires". The wireless broadcasts can in principle be of both the AM and FM variety due to the magnetic flux periodicity in electron energy spectra in bacterial DNA orbital motions.

  3. Engineering &Technology

    E-Print Network [OSTI]

    Southampton, University of

    Software Technologies Deloitte Dialog Semiconductor ECM Selection EDT-Year in Industry EMC Corporation to join our organisation and be based in our Ferndown, Dorset, location within our product electronics have application, design and manufacturing facilities in Canada, America, Europe and China. We

  4. Manufacturing technologies

    SciTech Connect (OSTI)

    1995-09-01

    The Manufacturing Technologies Center is an integral part of Sandia National Laboratories, a multiprogram engineering and science laboratory, operated for the Department of Energy (DOE) with major facilities at Albuquerque, New Mexico, and Livermore, California. Our Center is at the core of Sandia`s Advanced Manufacturing effort which spans the entire product realization process.

  5. Pyroprocessing Technologies

    E-Print Network [OSTI]

    Kemner, Ken

    of pyrochemical processes for the recycle of oxide, carbide and other advanced fuels and laid the foundationPyroprocessing Technologies RECYCLING USED NUCLEAR FUEL FOR A SUSTAINABLE ENERGY FUTURE #12;32 Storing Used Nuclear Fuel is a Real Waste Nuclear power is the most environmentally friendly way

  6. Channel plate for DNA sequencing

    DOE Patents [OSTI]

    Douthart, R.J.; Crowell, S.L.

    1998-01-13

    This invention is a channel plate that facilitates data compaction in DNA sequencing. The channel plate has a length, a width and a thickness, and further has a plurality of channels that are parallel. Each channel has a depth partially through the thickness of the channel plate. Additionally an interface edge permits electrical communication across an interface through a buffer to a deposition membrane surface. 15 figs.

  7. Building Technologies Office Overview

    SciTech Connect (OSTI)

    2013-04-01

    Building Technologies Office Overview Presentation for the 2013 Building Technologies Office's Program Peer Review

  8. Vacuum Technology

    SciTech Connect (OSTI)

    Biltoft, P J

    2004-10-15

    The environmental condition called vacuum is created any time the pressure of a gas is reduced compared to atmospheric pressure. On earth we typically create a vacuum by connecting a pump capable of moving gas to a relatively leak free vessel. Through operation of the gas pump the number of gas molecules per unit volume is decreased within the vessel. As soon as one creates a vacuum natural forces (in this case entropy) work to restore equilibrium pressure; the practical effect of this is that gas molecules attempt to enter the evacuated space by any means possible. It is useful to think of vacuum in terms of a gas at a pressure below atmospheric pressure. In even the best vacuum vessels ever created there are approximately 3,500,000 molecules of gas per cubic meter of volume remaining inside the vessel. The lowest pressure environment known is in interstellar space where there are approximately four molecules of gas per cubic meter. Researchers are currently developing vacuum technology components (pumps, gauges, valves, etc.) using micro electro mechanical systems (MEMS) technology. Miniature vacuum components and systems will open the possibility for significant savings in energy cost and will open the doors to advances in electronics, manufacturing and semiconductor fabrication. In conclusion, an understanding of the basic principles of vacuum technology as presented in this summary is essential for the successful execution of all projects that involve vacuum technology. Using the principles described above, a practitioner of vacuum technology can design a vacuum system that will achieve the project requirements.

  9. Science &Technology Facilities Council

    E-Print Network [OSTI]

    Science &Technology Facilities Council Science &Technology Facilities Council Science and Technology Facilities Council Annual Report and Accounts 2011-2012 Science and Technology Facilities Council Laboratory, Cheshire; UK Astronomy Technology Centre, Edinburgh; Chilbolton Observatory, Hampshire; Isaac

  10. SELECTING INFORMATION TECHNOLOGY SECURITY

    E-Print Network [OSTI]

    April 2004 SELECTING INFORMATION TECHNOLOGY SECURITY PRODUCTS Shirley Radack, Editor Computer Security Division Information Technology Laboratory National Institute of Standards and Technology Information technology security prod ucts are essential to better secure infor mation technology (IT) systems

  11. The dynamic interplay between DNA damage and metabolism : the metabolic fate and transport of DNA lesions and novel DNA damage derived from intermediary metabolism

    E-Print Network [OSTI]

    Jumpathong, Watthanachai

    2014-01-01

    The work presented in this thesis explores two novel and complementary facets of endogenous DNA damage: the development of biomarkers of inflammation based on metabolites of DNA damage products and the formation of DNA ...

  12. Portal Technology

    E-Print Network [OSTI]

    Warner, Beth Forrest

    2002-03-27

    Portal Technology Beth Forrest Warner Director, KU Digital Library Initiatives bwarner@ku.edu PUAD 839 March 27, 2002 Defining the issue… Today’s government agencies at all levels should note that the citizens they serve are “little concerned... their citizens’ perspectives. Instead of launching online services on a department-by-department basis, they are aggregating services across departments, accessible through a common portal.” (Janet Caldow, “The Quest for Electronic Government: A Defining...

  13. Technology disrupted

    SciTech Connect (OSTI)

    Papatheodorou, Y.

    2007-02-15

    Three years ago, the author presented a report on power generation technologies which in summary said 'no technology available today has the potential of becoming transformational or disruptive in the next five to ten years'. In 2006 the company completed another strategic view research report covering the electric power, oil, gas and unconventional energy industries and manufacturing industry. This article summarises the strategic view findings and then revisits some of the scenarios presented in 2003. The cost per megawatt-hour of the alternatives is given for plants ordered in 2005 and then in 2025. The issue of greenhouse gas regulation is dealt with through carbon sequestration and carbon allowances or an equivalent carbon tax. Results reveal substantial variability through nuclear power, hydro, wind, geothermal and biomass remain competitive through every scenario. Greenhouse gas scenario analysis shows coal still be viable, albeit less competitive against nuclear and renewable technologies. A carbon tax or allowance at $24 per metric ton has the same effect on IGCC cost as a sequestration mandate. However, the latter would hurt gas plants much more than a tax or allowance. Sequestering CO{sub 2} from a gas plant is almost as costly per megawatt-hour as for coal. 5 refs., 5 figs., 5 tabs.

  14. Photochemistry of psoralen-DNA adducts, biological effects of psoralen-DNA adducts, applications of psoralen-DNA photochemistry

    SciTech Connect (OSTI)

    Shi, Yun-bo

    1988-03-01

    This thesis consists of three main parts and totally eight chapters. In Part I, The author will present studies on the photochemistry of psoralen-DNA adducts, specifically, the wavelength dependencies for the photoreversals of thymidine-HMT (4'-hydroxymethyl-4, 5', 8-trimenthylpsoralen) monoadducts and diadduct and the same adducts incorporated in DNA helices and the wavelength dependecies for the photocrossslinking of thymidine-HMT monoadducts in double-stranded helices. In Part II, The author will report some biological effects of psoralen-DNA adducts, i.e., the effects on double-stranded DNA stability, DNA structure, and transcription by E. coli and T7 RNA polymerases. Finally, The author will focus on the applications of psoralen-DNA photochemistry to investigation of protein-DNA interaction during transcription, which includes the interaction of E. coli and T7 RNA polymerases with DNA in elongation complexes arrested at specific psoralen-DNA adduct sites as revealed by DNase I footprinting experiments. 123 refs., 52 figs., 12 tabs.

  15. Venus Technology Plan Venus Technology Plan

    E-Print Network [OSTI]

    Rathbun, Julie A.

    Venus Technology Plan May 2014 #12; ii Venus Technology Plan At the Venus Exploration Survey priorities, and (3) develop a Technology Plan for future Venus missions (after a Technology Forum at VEXAG Meeting 11 in November 2013). Here, we present the 2014 Venus Technology Plan

  16. DNA Sequencing Using capillary Electrophoresis

    SciTech Connect (OSTI)

    Dr. Barry Karger

    2011-05-09

    The overall goal of this program was to develop capillary electrophoresis as the tool to be used to sequence for the first time the Human Genome. Our program was part of the Human Genome Project. In this work, we were highly successful and the replaceable polymer we developed, linear polyacrylamide, was used by the DOE sequencing lab in California to sequence a significant portion of the human genome using the MegaBase multiple capillary array electrophoresis instrument. In this final report, we summarize our efforts and success. We began our work by separating by capillary electrophoresis double strand oligonucleotides using cross-linked polyacrylamide gels in fused silica capillaries. This work showed the potential of the methodology. However, preparation of such cross-linked gel capillaries was difficult with poor reproducibility, and even more important, the columns were not very stable. We improved stability by using non-cross linked linear polyacrylamide. Here, the entangled linear chains could move when osmotic pressure (e.g. sample injection) was imposed on the polymer matrix. This relaxation of the polymer dissipated the stress in the column. Our next advance was to use significantly lower concentrations of the linear polyacrylamide that the polymer could be automatically blown out after each run and replaced with fresh linear polymer solution. In this way, a new column was available for each analytical run. Finally, while testing many linear polymers, we selected linear polyacrylamide as the best matrix as it was the most hydrophilic polymer available. Under our DOE program, we demonstrated initially the success of the linear polyacrylamide to separate double strand DNA. We note that the method is used even today to assay purity of double stranded DNA fragments. Our focus, of course, was on the separation of single stranded DNA for sequencing purposes. In one paper, we demonstrated the success of our approach in sequencing up to 500 bases. Other application papers of sequencing up to this level were also published in the mid 1990's. A major interest of the sequencing community has always been read length. The longer the sequence read per run the more efficient the process as well as the ability to read repeat sequences. We therefore devoted a great deal of time to studying the factors influencing read length in capillary electrophoresis, including polymer type and molecule weight, capillary column temperature, applied electric field, etc. In our initial optimization, we were able to demonstrate, for the first time, the sequencing of over 1000 bases with 90% accuracy. The run required 80 minutes for separation. Sequencing of 1000 bases per column was next demonstrated on a multiple capillary instrument. Our studies revealed that linear polyacrylamide produced the longest read lengths because the hydrophilic single strand DNA had minimal interaction with the very hydrophilic linear polyacrylamide. Any interaction of the DNA with the polymer would lead to broader peaks and lower read length. Another important parameter was the molecular weight of the linear chains. High molecular weight (> 1 MDA) was important to allow the long single strand DNA to reptate through the entangled polymer matrix. In an important paper, we showed an inverse emulsion method to prepare reproducibility linear polyacrylamide polymer with an average MWT of 9MDa. This approach was used in the polymer for sequencing the human genome. Another critical factor in the successful use of capillary electrophoresis for sequencing was the sample preparation method. In the Sanger sequencing reaction, high concentration of salts and dideoxynucleotide remained. Since the sample was introduced to the capillary column by electrokinetic injection, these salt ions would be favorably injected into the column over the sequencing fragments, thus reducing the signal for longer fragments and hence reading read length. In two papers, we examined the role of individual components from the sequencing reaction and then developed a protocol to reduce the deleterio

  17. Photoelectrochemical synthesis of DNA microarrays

    E-Print Network [OSTI]

    Jacobson, Joseph

    Optical addressing of semiconductor electrodes represents a powerful technology that enables the independent and parallel control of a very large number of electrical phenomena at the solid-electrolyte interface. To date, ...

  18. DNA

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration would like submit theCovalent Bonding Low-Cost Ground8 GasDEVELOPMENTS E P I IT h it cdrives

  19. DNA

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    blueprint of a bacterium's "molecular machinery," showing how bacterial immune systems fight off the viruses that infect them. By tracking down how bacterial defense systems work,...

  20. Base Excision by Thymine DNA Glycosylase Mediates DNA-Directed Cytotoxicity of 5-Fluorouracil 

    E-Print Network [OSTI]

    Selfridge J.; Schar P.; Lettieri T.; Schuermann D.; Saito Y.; Focke F.; Kunz C.

    2009-04-01

    5-Fluorouracil (5-FU), a chemotherapeutic drug commonly used in cancer treatment, imbalances nucleotide pools, thereby favoring misincorporation of uracil and 5-FU into genomic DNA. The processing of these bases by DNA repair activities was proposed...

  1. Protein-DNA Interactions Determine the Shapes of DNA Toroids Condensed in Virus Capsids

    E-Print Network [OSTI]

    Podgornik, Rudolf

    Protein-DNA Interactions Determine the Shapes of DNA Toroids Condensed in Virus Capsids Ame (13), or the virus capsid itself (14­16), either upon addition of spermine (Spm4þ ) or in a monovalent

  2. DNA binding specificity of the p73 DNA-binding domain

    E-Print Network [OSTI]

    Tse, Pui Wah

    2011-01-01

    of DNA recognition by p53 tetramers. Mol Cell 22, 741-753.site as a self-assembled tetramer. Structure 18, 246- Chene,structure of a p53 core tetramer bound to DNA. Oncogene 28,

  3. DNA-guided nanoparticle assemblies

    DOE Patents [OSTI]

    Gang, Oleg; Nykypanchuk, Dmytro; Maye, Mathew; van der Lelie, Daniel

    2013-07-16

    In some embodiments, DNA-capped nanoparticles are used to define a degree of crystalline order in assemblies thereof. In some embodiments, thermodynamically reversible and stable body-centered cubic (bcc) structures, with particles occupying <.about.10% of the unit cell, are formed. Designs and pathways amenable to the crystallization of particle assemblies are identified. In some embodiments, a plasmonic crystal is provided. In some aspects, a method for controlling the properties of particle assemblages is provided. In some embodiments a catalyst is formed from nanoparticles linked by nucleic acid sequences and forming an open crystal structure with catalytically active agents attached to the crystal on its surface or in interstices.

  4. DNA: The Strand that Connects Us All

    SciTech Connect (OSTI)

    Kaplan, Matt (University of Arizona Genetics Core) [University of Arizona Genetics Core

    2011-03-29

    Learn how the methods and discoveries of human population genetics are applied for personal genealogical reconstruction and anthropological testing. Dr. Kaplan starts with a short general review of human genetics and the biology behind this form of DNA testing. He looks at how DNA testing is performed and how samples are processed in the University of Arizona laboratory. He also examines examples of personal genealogical results from Family Tree DNA and personal anthropological results from the Genographic Project. Finally, he describes the newest project in the UA laboratory, the DNA Shoah Project.

  5. DNA sequencing using fluorescence background electroblotting membrane

    DOE Patents [OSTI]

    Caldwell, K.D.; Chu, T.J.; Pitt, W.G.

    1992-05-12

    A method for the multiplex sequencing on DNA is disclosed which comprises the electroblotting or specific base terminated DNA fragments, which have been resolved by gel electrophoresis, onto the surface of a neutral non-aromatic polymeric microporous membrane exhibiting low background fluorescence which has been surface modified to contain amino groups. Polypropylene membranes are preferably and the introduction of amino groups is accomplished by subjecting the membrane to radio or microwave frequency plasma discharge in the presence of an aminating agent, preferably ammonia. The membrane, containing physically adsorbed DNA fragments on its surface after the electroblotting, is then treated with crosslinking means such as UV radiation or a glutaraldehyde spray to chemically bind the DNA fragments to the membrane through amino groups contained on the surface. The DNA fragments chemically bound to the membrane are subjected to hybridization probing with a tagged probe specific to the sequence of the DNA fragments. The tagging may be by either fluorophores or radioisotopes. The tagged probes hybridized to the target DNA fragments are detected and read by laser induced fluorescence detection or autoradiograms. The use of aminated low fluorescent background membranes allows the use of fluorescent detection and reading even when the available amount of DNA to be sequenced is small. The DNA bound to the membranes may be reprobed numerous times. No Drawings

  6. SnapShot: DNA Polymerases II Mammals

    E-Print Network [OSTI]

    Foti, James J.

    DNA polymerases ensure the faithful duplication of genetic information inside the nuclease and mitochondria of eukaryotic cells and the nucleoid of prokaryotic cells. These remarkable enzymes synthesize polynucleotide ...

  7. Technology and the Box

    E-Print Network [OSTI]

    Maitland, Padma

    2013-01-01

    its explorations of technology in partnership with radicalPadma Maitland Technology and the Box The room is thedisciplines. The theme of “Technology and the Box” emerged

  8. Information Technology and Libraries

    E-Print Network [OSTI]

    Hubble, Ann; Murphy, Deborah A.; Perry, Susan Chesley

    2011-01-01

    Sue Chesley Perry 196 INFORMATION TECHNOLOGY AND LIBRARIES |LITA - Library & Information Technology Association). ”Two of the 190 INFORMATION TECHNOLOGY AND LIBRARIES |

  9. Nuclear Science & Technology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Nuclear Science & Technology Nuclear Science & Technology1354608000000Nuclear Science & TechnologySome of these resources are LANL-only and will require Remote Access. No...

  10. Available Technologies

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 OutreachProductswsicloudwsiclouddenDVA N C E D B L O O DBiomass andAtomsVehicles and Fuels VehiclesTechnologies

  11. DNA Profiling Using Solid-State Nanopores: Detection of DNA-Binding

    E-Print Network [OSTI]

    Meller, Amit

    a 3.5 nm pore results from threading of a dye-intercalated DNA molecule, as compared to the typical for drug development, necessitating new in vitro methods for rapid and low-cost assessment of the binding molecules, which give the DNA/intercalator complex a bulkier structure than that of native DNA. Furthermore

  12. Ancient DNA Chronology within Sediment Deposits: Are Paleobiological Reconstructions Possible and Is DNA Leaching a Factor?

    E-Print Network [OSTI]

    Nielsen, Rasmus

    Ancient DNA Chronology within Sediment Deposits: Are Paleobiological Reconstructions Possible reported the successful extraction of ancient DNA (aDNA) from both frozen and nonfrozen sediments (even sediments up to 3300 years old at 2 cave sites in the North Island of New Zealand. These sites are ideal

  13. Dellaporta DNA Extraction Citation: Stephen L. Dellaporta,Jonathan Wood , James B. Hicks. A plant DNA

    E-Print Network [OSTI]

    Wurtele, Eve Syrkin

    1 Dellaporta DNA Extraction Citation: Stephen L. Dellaporta,Jonathan Wood , James B. Hicks. A plant supernatant and lightly dry DNA pellets by inverting the tubes on paper towels for 10 min. #12;4 12. Redissolve each DNA pellet with 0.7 mL EB2. May need to let sit overnight at 4°C if having trouble dissolving

  14. Distributed Energy Technology Characterization (Desiccant Technologies...

    Office of Environmental Management (EM)

    technologies can be designed to utilize the available thermal energy from a combined heat and power (CHP) system. This technology characterization is intended to provide...

  15. Vehicle Technologies Office: 2014 Electric Drive Technologies...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    automotive technologies under development. Research is focused on developing power electronics (PE), electric motor, and traction drive system (TDS) technologies that will reduce...

  16. NATIONAL ENERGY TECHNOLOGY LABORATORY Technology Transfer Basic...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    in Technology Transfer" award from the Federal Laboratory Consortium. Application of this technology reduces the costs and energy associated with more conventional scrubbing...

  17. Technology Support Bob Davis

    E-Print Network [OSTI]

    Technology Support Services · Bob Davis · Associate Director User Support Services 1 #12;Technology Support Services · NUIT Technology Support Services (TSS) helps Northwestern faculty, staff, and students Technologies · Brian Nielsen · Project Manager Faculty Initiatives 8 #12;Support for Teaching & Learning

  18. NREL: Technology Deployment - Technology Acceleration

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration wouldMass map shines lightGeospatial ToolkitSMARTS -Being Replicated AcrossSolarTechnology

  19. Allostery through protein-induced DNA bubbles

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Traverso, Joseph J.; Manoranjan, Valipuram S.; Bishop, A. R.; Rasmussen, Kim Ø.; Voulgarakis, Nikolaos K.

    2015-03-12

    Allostery through DNA is increasingly recognized as an important modulator of DNA functions. Here, we show that the coalescence of protein-induced DNA bubbles can mediate allosteric interactions that drive protein aggregation. We propose that such allostery may regulate DNA's flexibility and the assembly of the transcription machinery. Mitochondrial transcription factor A (TFAM), a dual-function protein involved in mitochondrial DNA (mtDNA) packaging and transcription initiation, is an ideal candidate to test such a hypothesis owing to its ability to locally unwind the double helix. Numerical simulations demonstrate that the coalescence of TFAM-induced bubbles can explain experimentally observed TFAM oligomerization. The resultingmore »melted DNA segment, approximately 10 base pairs long, around the joints of the oligomers act as flexible hinges, which explains the efficiency of TFAM in compacting DNA. Since mitochondrial polymerase (mitoRNAP) is involved in melting the transcription bubble, TFAM may use the same allosteric interaction to both recruit mitoRNAP and initiate transcription.« less

  20. DNA Nanomechanical Switches under Folding Kinetics Control

    E-Print Network [OSTI]

    Meller, Amit

    DNA Nanomechanical Switches under Folding Kinetics Control Virgile Viasnoff,, Amit Meller operate at equilibrium under changes in solution composition. We propose an alternative DNA switch design after heat denaturation drives the switch to its lowest energy conformation, while rapid cooling (>100

  1. Dynamics and control of DNA sequence amplification

    SciTech Connect (OSTI)

    Marimuthu, Karthikeyan [Department of Chemical Engineering and Center for Advanced Process Decision-Making, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213 (United States); Chakrabarti, Raj, E-mail: raj@pmc-group.com, E-mail: rajc@andrew.cmu.edu [Department of Chemical Engineering and Center for Advanced Process Decision-Making, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213 (United States); Division of Fundamental Research, PMC Advanced Technology, Mount Laurel, New Jersey 08054 (United States)

    2014-10-28

    DNA amplification is the process of replication of a specified DNA sequence in vitro through time-dependent manipulation of its external environment. A theoretical framework for determination of the optimal dynamic operating conditions of DNA amplification reactions, for any specified amplification objective, is presented based on first-principles biophysical modeling and control theory. Amplification of DNA is formulated as a problem in control theory with optimal solutions that can differ considerably from strategies typically used in practice. Using the Polymerase Chain Reaction as an example, sequence-dependent biophysical models for DNA amplification are cast as control systems, wherein the dynamics of the reaction are controlled by a manipulated input variable. Using these control systems, we demonstrate that there exists an optimal temperature cycling strategy for geometric amplification of any DNA sequence and formulate optimal control problems that can be used to derive the optimal temperature profile. Strategies for the optimal synthesis of the DNA amplification control trajectory are proposed. Analogous methods can be used to formulate control problems for more advanced amplification objectives corresponding to the design of new types of DNA amplification reactions.

  2. Recombinant DNA encoding a desulfurization biocatalyst

    DOE Patents [OSTI]

    Rambosek, J.; Piddington, C.S.; Kovacevich, B.R.; Young, K.D.; Denome, S.A.

    1994-10-18

    This invention relates to a recombinant DNA molecule containing a gene or genes which encode a biocatalyst capable of desulfurizing a fossil fuel which contains organic sulfur molecules. For example, the present invention encompasses a recombinant DNA molecule containing a gene or genes of a strain of Rhodococcus rhodochrous. 13 figs.

  3. Prospects & Overviews Integrating DNA barcode data and

    E-Print Network [OSTI]

    DeSalle, Rob

    , and description Paul Z. Goldstein and Rob DeSalleà DNA barcodes, like traditional sources of taxonomic information interpretation. The role of DNA barcoding in generating hypotheses of new taxa in need of formal taxonomic information but also for our comprehension of the magnitude of species diversity and its disappearance

  4. Technologies de base Architectures

    E-Print Network [OSTI]

    Grigoras, .Romulus

    Technologies de base Architectures Cinquième partie Technologies Web Intergiciels et applications communicantes 1 / 38 #12;Technologies de base Architectures Client-serveur HTTP Présentation Plan 1 Technologies Contenu dynamique 2-tier 3-tier V ­ Technologies Web 2 / 38 #12;Technologies de base Architectures Client

  5. The shape of the DNA minor groove directs binding by the DNA-bending protein Fis

    SciTech Connect (OSTI)

    Stella, Stefano; Cascio, Duilio; Johnson, Reid C.

    2010-06-21

    The bacterial nucleoid-associated protein Fis regulates diverse reactions by bending DNA and through DNA-dependent interactions with other control proteins and enzymes. In addition to dynamic nonspecific binding to DNA, Fis forms stable complexes with DNA segments that share little sequence conservation. Here we report the first crystal structures of Fis bound to high- and low-affinity 27-base-pair DNA sites. These 11 structures reveal that Fis selects targets primarily through indirect recognition mechanisms involving the shape of the minor groove and sequence-dependent induced fits over adjacent major groove interfaces. The DNA shows an overall curvature of {approx}65{sup o}, and the unprecedented close spacing between helix-turn-helix motifs present in the apodimer is accommodated by severe compression of the central minor groove. In silico DNA structure models show that only the roll, twist, and slide parameters are sufficient to reproduce the changes in minor groove widths and recreate the curved Fis-bound DNA structure. Models based on naked DNA structures suggest that Fis initially selects DNA targets with intrinsically narrow minor grooves using the separation between helix-turn-helix motifs in the Fis dimer as a ruler. Then Fis further compresses the minor groove and bends the DNA to generate the bound structure.

  6. Elastic and Proton Dynamics of the DNA

    E-Print Network [OSTI]

    V. L. Golo

    2008-03-28

    The subject of this report is the dynamics of elastic system in conjunction with hydrogen bonds of the DNA. We draw attention to the draw-back of the familiar rod model of the DNA, and make a case of constructing models that could accommodate the intrinsic structure of the DNA. In this respect studying the interplay among the elastic system and the protons of the DNA, is of interest, for it could accommodate the inter-strand as well as the tunneling modes of protons. Following this direction, we come to the conclusion that the elastic-proton dynamics may have a bearing on biophysics of the DNA. The phenomenon of point mutations is discussed within this framework.

  7. Programmable DNA-mediated multitasking processor

    E-Print Network [OSTI]

    Shu, Jian-Jun; Yong, Kian-Yan; Shao, Fangwei; Lee, Kee Jin

    2015-01-01

    Because of DNA appealing features as perfect material, including minuscule size, defined structural repeat and rigidity, programmable DNA-mediated processing is a promising computing paradigm, which employs DNAs as information storing and processing substrates to tackle the computational problems. The massive parallelism of DNA hybridization exhibits transcendent potential to improve multitasking capabilities and yield a tremendous speed-up over the conventional electronic processors with stepwise signal cascade. As an example of multitasking capability, we present an in vitro programmable DNA-mediated optimal route planning processor as a functional unit embedded in contemporary navigation systems. The novel programmable DNA-mediated processor has several advantages over the existing silicon-mediated methods, such as conducting massive data storage and simultaneous processing via much fewer materials than conventional silicon devices.

  8. Method for sequencing DNA base pairs

    DOE Patents [OSTI]

    Sessler, A.M.; Dawson, J.

    1993-12-14

    The base pairs of a DNA structure are sequenced with the use of a scanning tunneling microscope (STM). The DNA structure is scanned by the STM probe tip, and, as it is being scanned, the DNA structure is separately subjected to a sequence of infrared radiation from four different sources, each source being selected to preferentially excite one of the four different bases in the DNA structure. Each particular base being scanned is subjected to such sequence of infrared radiation from the four different sources as that particular base is being scanned. The DNA structure as a whole is separately imaged for each subjection thereof to radiation from one only of each source. 6 figures.

  9. TECHNOLOGY LICENSE APPLICATION Office of Technology Transfer

    E-Print Network [OSTI]

    Page 1 TECHNOLOGY LICENSE APPLICATION Office of Technology Transfer UT-Battelle, LLC (UT. One of the functions of UT-BATTELLE's Office of Technology Transfer is to negotiate license agreements for such intellectual property with companies for commercial applications of ORNL-developed technologies. Such licenses

  10. APPROPRIATE HOME TECHNOLOGY: Depending on Dependable Technology

    E-Print Network [OSTI]

    Sommerville, Ian

    penetrate more and more into people's everyday lives and homes, the `design problem' is not so muchAPPROPRIATE HOME TECHNOLOGY: Depending on Dependable Technology Systems Guy Dewsbury, Karen Clarke 2002 #12;Dewsbury et al (2002): Appropriate Home Technology APPROPRIATE HOME TECHNOLOGY: Depending

  11. Particulate Carrier Systems for Mucosal DNA Vaccine Delivery

    E-Print Network [OSTI]

    Borchard, Gerrit

    2006-10-26

    Streptomyces griseus Stop solution: 1M KOH In humans: degradation by lysozyme Incubation with chitosanase (1) GPEN 2006 Free DNA chitoplexes Incubation with chitosanase, 37?C Intact DNA ? Degraded chitosan Intact DNA ? Extraction with phenol: chloroform... Streptomyces griseus Stop solution: 1M KOH In humans: degradation by lysozyme Incubation with chitosanase (1) GPEN 2006 Free DNA chitoplexes Incubation with chitosanase, 37?C Intact DNA ? Degraded chitosan Intact DNA ? Extraction with phenol: chloroform...

  12. Microfluidics: Kinetics of Hybridized DNA With Fluid Flow Variations...

    Office of Scientific and Technical Information (OSTI)

    Conference: Microfluidics: Kinetics of Hybridized DNA With Fluid Flow Variations. Citation Details In-Document Search Title: Microfluidics: Kinetics of Hybridized DNA With Fluid...

  13. Protein Bridges DNA Base and Nucleotide Excision Repair Pathways

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Bridges DNA Base and Nucleotide Excision Repair Pathways Print Alkyltransferase proteins (AGT) protect cells from the biological effects of DNA damage caused by the addition...

  14. IN VITRO MUTAGENIC AND DNA AND CHROMOSOMAL DAMAGE ACTIVITY BY...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    IN VITRO MUTAGENIC AND DNA AND CHROMOSOMAL DAMAGE ACTIVITY BY SURFACTANT DISPERSION OR SOLVENT EXTRACT OF A REFERENCE DIESEL EXHAUST PARTICULATE MATERIAL IN VITRO MUTAGENIC AND DNA...

  15. Topoisomerase II Structure Suggests Novel DNA Cleavage Mechanism

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Topoisomerase II Structure Suggests Novel DNA Cleavage Mechanism Print Type II topoisomerases are molecular machines that regulate DNA supercoiling and separate interlocked...

  16. Charge-transport-mediated recruitment of DNA repair enzymes Pak-Wing Fok,1,2,a

    E-Print Network [OSTI]

    Levine, Alex J.

    Charge-transport-mediated recruitment of DNA repair enzymes Pak-Wing Fok,1,2,a Chin-Lin Guo,3 and Tom Chou2,4,b 1 Applied and Computational Mathematics, California Institute of Technology, Pasadena, California 91125, USA 2 Department of Biomathematics, UCLA, Los Angeles, California 90095-1766, USA 3 Applied

  17. Gel Electrophoresis of Gold-DNA Nanoconjugates

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Pellegrino, T.; Sperling, R. A.; Alivisatos, A. P.; Parak, W. J.

    2007-01-01

    Gold-DNA conjugates were investigated in detail by a comprehensive gel electrophoresis study based on 1200 gels. A controlled number of single-stranded DNA of different length was attached specifically via thiol-Au bonds to phosphine-stabilized colloidal gold nanoparticles. Alternatively, the surface of the gold particles was saturated with single stranded DNA of different length either specifically via thiol-Au bonds or by nonspecific adsorption. From the experimentally determined electrophoretic mobilities, estimates for the effective diameters of the gold-DNA conjugates were derived by applying two different data treatment approaches. The first method is based on making a calibration curve for the relation between effectivemore »diameters and mobilities with gold nanoparticles of known diameter. The second method is based on Ferguson analysis which uses gold nanoparticles of known diameter as reference database. Our study shows that effective diameters derived from gel electrophoresis measurements are affected with a high error bar as the determined values strongly depend on the method of evaluation, though relative changes in size upon binding of molecules can be detected with high precision. Furthermore, in this study, the specific attachment of DNA via gold-thiol bonds to Au nanoparticles is compared to nonspecific adsorption of DNA. Also, the maximum number of DNA molecules that can be bound per particle was determined.« less

  18. Method of quantitating dsDNA

    DOE Patents [OSTI]

    Stark, Peter C. (Los Alamos, NM); Kuske, Cheryl R. (Los Alamos, NM); Mullen, Kenneth I. (Los Alamos, NM)

    2002-01-01

    A method for quantitating dsDNA in an aqueous sample solution containing an unknown amount of dsDNA. A first aqueous test solution containing a known amount of a fluorescent dye-dsDNA complex and at least one fluorescence-attenutating contaminant is prepared. The fluorescence intensity of the test solution is measured. The first test solution is diluted by a known amount to provide a second test solution having a known concentration of dsDNA. The fluorescence intensity of the second test solution is measured. Additional diluted test solutions are similarly prepared until a sufficiently dilute test solution having a known amount of dsDNA is prepared that has a fluorescence intensity that is not attenuated upon further dilution. The value of the maximum absorbance of this solution between 200-900 nanometers (nm), referred to herein as the threshold absorbance, is measured. A sample solution having an unknown amount of dsDNA and an absorbance identical to that of the sufficiently dilute test solution at the same chosen wavelength is prepared. Dye is then added to the sample solution to form the fluorescent dye-dsDNA-complex, after which the fluorescence intensity of the sample solution is measured and the quantity of dsDNA in the sample solution is determined. Once the threshold absorbance of a sample solution obtained from a particular environment has been determined, any similarly prepared sample solution taken from a similar environment and having the same value for the threshold absorbance can be quantified for dsDNA by adding a large excess of dye to the sample solution and measuring its fluorescence intensity.

  19. Nanopores formed by DNA origami: a review

    E-Print Network [OSTI]

    Bell, Nicholas A. W.; Keyser, Ulrich F.

    2014-06-10

    , coated with hydrophobic moieties, into a lipid bilayer. Recent work in these two branches is now discussed. Hybrid nanopores formed by trapping DNA origami onto a solid state nanopore The combination of DNA origami and solid state nanopores was first... (1982) Nucleic acid junctions and lattices. J. Theor. Biol. 99, 237–47. 28 Rothemund PWK (2006) Folding DNA to create nanoscale shapes and patterns. Nature 440, 297– 302. 29 Kuzyk A, Schreiber R, Fan Z, Pardatscher G, Roller E-M, Högele A, Simmel FC...

  20. Workshop on induced Seismicity due to fluid injection/production from Energy-Related Applications

    E-Print Network [OSTI]

    Majer, E.L.

    2011-01-01

    Ormat MIT ATI Nuclear Regulatory Commission LLNL USGS/Ormat MIT ATI Nuclear Regulatory Commission LLNL USGS/

  1. Plasma technology directory

    SciTech Connect (OSTI)

    Ward, P.P.; Dybwad, G.L.

    1995-03-01

    The Plasma Technology Directory has two main goals: (1) promote, coordinate, and share plasma technology experience and equipment within the Department of Energy; and (2) facilitate technology transfer to the commercial sector where appropriate. Personnel are averaged first by Laboratory and next by technology area. The technology areas are accelerators, cleaning and etching deposition, diagnostics, and modeling.

  2. Technology Application Centers: Facilitating Technology Transfer 

    E-Print Network [OSTI]

    Kuhel, G. J.

    1994-01-01

    Industrial DSM programs cannot succeed unless customers learn about and implement new technologies in a timely manner. Why? Because this expeditious transfer of new technologies represents the key challenge for the 1990s. This paper explores...

  3. DNA Assembly Line for Nano-Construction

    ScienceCinema (OSTI)

    Oleg Gang

    2010-01-08

    Building on the idea of using DNA to link up nanoparticles scientists at Brookhaven National Lab have designed a molecular assembly line for high-precision nano-construction. Nanofabrication is essential for exploiting the unique properties of nanoparticl

  4. Casting inorganic structures with DNA molds

    E-Print Network [OSTI]

    Sun, Wei

    We report a general strategy for designing and synthesizing inorganic nanostructures with arbitrarily prescribed three-dimensional shapes. Computationally designed DNA strands self-assemble into a stiff “nanomold” that ...

  5. Deoxyribose oxidation chemistry and endogenous DNA adducts

    E-Print Network [OSTI]

    Zhou, Xinfeng

    2006-01-01

    Endogenous and exogenous oxidants react with cellular macromolecules to generate a variety of electrophiles that react with DNA produce cytotoxic and mutagenic adducts. One source of such electrophiles is deoxyribose in ...

  6. Ubiquitylation, neddylation and the DNA damage response

    E-Print Network [OSTI]

    Brown, Jessica S.; Jackson, Stephen P.

    2015-04-01

    , collectively termed the DNA damage response (DDR), requires the recruitment and subsequent post-translational modification (PTM) of a complex network of proteins. Ubiquitin and the ubiquitin-like protein (UBL) SUMO have established roles in regulating...

  7. Intriguing DNA Editor Has a Structural Trigger

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    "plug in" very specific snippets of DNA coding for a variety of reasons. For example, microbes could be re-engineered to consume specific environmental toxins or to produce better...

  8. Linear Thermodynamics of Rodlike DNA Filtration

    E-Print Network [OSTI]

    Li, Zirui

    Linear thermodynamics transportation theory is employed to study filtration of rodlike DNA molecules. Using the repeated nanoarray consisting of alternate deep and shallow regions, it is demonstrated that the complex ...

  9. Extracting biological knowledge from DNA sequences

    SciTech Connect (OSTI)

    De La Vega, F.M.; Thieffry, D.; Collado-Vides, J.

    1996-12-31

    This session describes the elucidation of information from dna sequences and what challenges computational biologists face in their task of summarizing and deciphering the human genome. Techniques discussed include methods from statistics, information theory, artificial intelligence and linguistics. 1 ref.

  10. Euler buckling and nonlinear kinking of double-stranded DNA

    E-Print Network [OSTI]

    Cohen, Adam E.

    physiological conditions: at high curvature, does the DNA bend smoothly, or does it kink like a drinking straw and damage). These aspects of DNA mechanics are likely to influence protein binding and DNA packaging, yet the effect of single-nucleotide mismatches on DNA bending. Our data support a model of linear elastic bending

  11. The unholy trinity: taxonomy, species delimitation and DNA barcoding

    E-Print Network [OSTI]

    DeSalle, Rob

    The unholy trinity: taxonomy, species delimitation and DNA barcoding Rob DeSalle*, Mary G. Egan are clarified and resolved, before the use of DNA as a tool for taxonomy and species delimitation can framework for interweaving classical taxonomy with the goals of `DNA barcoding'. Keywords: DNA barcoding

  12. Synopsis: Repulsion Helps Virus Pack DNA APS/Joan Tycko

    E-Print Network [OSTI]

    Smith, Douglas E.

    Synopsis: Repulsion Helps Virus Pack DNA APS/Joan Tycko Repulsive DNA-DNA Interactions Accelerate, and Douglas E. Smith Phys. Rev. Lett. 112, 248101 (2014) Published June 17, 2014 Featured in Physics Editors reported in Physical Review Letters show that, surprisingly, switching the DNA self-interaction from

  13. DNA Concentration By UV Spectrophotometry Measure Absorption

    E-Print Network [OSTI]

    Aris, John P.

    DNA Concentration By UV Spectrophotometry Measure Absorption: 1. Dilute DNA to 0.5 to 50 µg/ml in TE buffer or dH2O. Plan to use a quartz cuvette or a UV-transparent plastic (disposable) cuvette (1 2. Measure absorption at 260 nm (A260). Start by zeroing instrument with TE buffer or dH2O alone

  14. Overview of DNA Programs at NIST

    E-Print Network [OSTI]

    .nist.gov/mml/bmd/genetics/applied_genetics_pubs.cfm Pete Vallone Erica Butts DNA Biometrics Team Funding from the FBI S&T Branch through NIST Information http://www.cstl.nist.gov/strbase/NISTpub.htm +FBI-funding (DNA biometrics) Workshops 0 0 0 1 2 7 9 6 11) measurement calibration Required under FBI Quality Assurance Standard 9.5.5 for labs connected to the national

  15. Carbon Nanotube DNA Sensor and Sensing Mechanism

    E-Print Network [OSTI]

    Le Roy, Robert J.

    nanotube (SWNT) DNA sensors and the sensing mechanism. The simple and generic protocol for label for direct label-free detection of DNA hybridization in a biocompatible buffer solution. We also carried out is a field effect device, which has a typical on-current of 3-6 µA at 10 mV source- drain bias and an on-off

  16. Technology and the Box

    E-Print Network [OSTI]

    Maitland, Padma

    2013-01-01

    study of architecture through references to “Technology andhis new “Architecture for Man” that combines technology withArchitecture and Minarc Architects, two contemporary designers that are pushing prefab technologies

  17. Adoption of New Technology

    E-Print Network [OSTI]

    Hall, Bronwyn H.; Khan, Beethika

    2003-01-01

    Firm Diffusion of New Technology: A Real Options Model. ”and the Adoption of New technology: Evidence from the U.S.affect whether or not new technologies are successful, the

  18. Technology & Engineering Division

    E-Print Network [OSTI]

    Technology & Engineering Division High-Temperature Superconducting Magnets for Fusion: New & Engineering Division Contents · Background on Superconductivity · Fusion Magnets ­ Present and Future ­ Vision/15/2014 2Joseph V. Minervini #12;Technology & Engineering Division Superconductivity #12;Technology

  19. Adoption of New Technology

    E-Print Network [OSTI]

    Hall, Bronwyn H.; Khan, Beethika

    2003-01-01

    Firm Diffusion of New Technology: A Real Options Model. ”and the Adoption of New technology: Evidence from the U.S.the Diffusion of New Technology in the Banking Industry. ”

  20. Denaturation of DNA at high salt concentrations

    E-Print Network [OSTI]

    Maity, Arghya; Singh, Navin

    2015-01-01

    Cations present in the solution are important for the stability of two negative strands of DNA molecules. Experimental as well as theoretical results show that the DNA molecule is more stable as the concentration of salt (or cations) increases. It is known that the two strands of DNA molecule carry negative charge due to phosphate group along the strands. These cations act as a shielding particles to the two like charge strands. Recently, in an experiment it is shown that there is a critical value in the concentration of salts (or cations) that can stabilize the helical structure of DNA. If one add more salt in the solution beyond this critical value, the stability of the DNA molecule will disrupt. In this work we study the stability of DNA molecules at higher concentrations. How the stability at higher concentration can be explained through some theoretical calculations is the aim of this manuscript. We consider the PBD model with proper modifications that can explain the negative stability of the molecule a...

  1. Denaturation of DNA at high salt concentrations

    E-Print Network [OSTI]

    Arghya Maity; Amar Singh; Navin Singh

    2015-08-19

    Cations present in the solution are important for the stability of two negative strands of DNA molecules. Experimental as well as theoretical results show that the DNA molecule is more stable as the concentration of salt (or cations) increases. It is known that the two strands of DNA molecule carry negative charge due to phosphate group along the strands. These cations act as a shielding particles to the two like charge strands. Recently, in an experiment it is shown that there is a critical value in the concentration of salts (or cations) that can stabilize the helical structure of DNA. If one add more salt in the solution beyond this critical value, the stability of the DNA molecule will disrupt. In this work we study the stability of DNA molecules at higher concentrations. How the stability at higher concentration can be explained through some theoretical calculations is the aim of this manuscript. We consider the PBD model with proper modifications that can explain the negative stability of the molecule at higher concentration. Our findings are in close match with the experimental results.

  2. Technology Readiness Assessment Report

    Office of Environmental Management (EM)

    of management decisions by identifying key technologies that have been demonstrated to work or by highlighting immature or unproven technologies that might result in increased...

  3. Promising Technologies List

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    about promising new and underutilized energy-saving technologies available for Federal and commercial building sector deployment. To identify promising technologies,...

  4. Building Technologies Office Overview

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Roland Risser Director, Building Technologies Office Building Technologies Office Overview Our Homes and Buildings Use 40% of Our Nation's Energy and 75% of Electricity Energy Use...

  5. Hydropower Program Technology Overview

    SciTech Connect (OSTI)

    Not Available

    2001-10-01

    New fact sheets for the DOE Office of Power Technologies (OPT) that provide technology overviews, description of DOE programs, and market potential for each OPT program area.

  6. Vehicle Technologies Office: News

    Broader source: Energy.gov [DOE]

    EERE intends to issue, on behalf of its Fuel Cell Technologies Office, a Funding Opportunity Announcement (FOA) entitled "Fuel Cell Technologies Incubator: Innovations in Fuel Cell and Hydrogen...

  7. Building Technologies Office Overview

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Technologies Office Roland Risser Director, Building Technologies Office National Energy Consumption 40% 60% Reducing consumption or improving performance calls for cutting-edge...

  8. Essays on University Technology Management

    E-Print Network [OSTI]

    Drivas, Kyriakos

    2011-01-01

    of university technology management and their implicationson University Technology Management by Kyriakos Drivas Aon University Technology Management by Kyriakos Drivas

  9. Morgantown Energy Technology Center, technology summary

    SciTech Connect (OSTI)

    Not Available

    1994-06-01

    This document has been prepared by the DOE Environmental Management (EM) Office of Technology Development (OTD) to highlight its research, development, demonstration, testing, and evaluation activities funded through the Morgantown Energy Technology Center (METC). Technologies and processes described have the potential to enhance DOE`s cleanup and waste management efforts, as well as improve US industry`s competitiveness in global environmental markets. METC`s R&D programs are focused on commercialization of technologies that will be carried out in the private sector. META has solicited two PRDAs for EM. The first, in the area of groundwater and soil technologies, resulted in twenty-one contact awards to private sector and university technology developers. The second PRDA solicited novel decontamination and decommissioning technologies and resulted in eighteen contract awards. In addition to the PRDAs, METC solicited the first EM ROA in 1993. The ROA solicited research in a broad range of EM-related topics including in situ remediation, characterization, sensors, and monitoring technologies, efficient separation technologies, mixed waste treatment technologies, and robotics. This document describes these technology development activities.

  10. 2009 Wind Technologies Market Report

    E-Print Network [OSTI]

    Wiser, Ryan

    2010-01-01

    land- based wind energy technology. 2009 Wind TechnologiesRenewable Energy 2009 WIND TECHNOLOGIES MARKET REPORT AUGUSTfor a variety of energy technologies, including wind energy.

  11. Technology Licensing | ornl.gov

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    License ORNL Technologies Licensing Guidelines NDA(s) and MTA(s) Sample Agreements Technology Innovation Program Technology Assistance Program Licensing Staff Technology Search...

  12. Training & Technology Solutions Queens College ~ Office of Information Technology ~ Training & Technology Solutions

    E-Print Network [OSTI]

    Johnson Jr.,, Ray

    Training & Technology Solutions Queens College ~ Office of Information Technology ~ Training & Technology Solutions 718-997-4875 ~ training@qc.cuny.edu ~ I-Bldg 214 Faculty Center Verification & Technology Solutions Queens College ~ Office of Information Technology ~ Training & Technology Solutions 718

  13. TOKYO INSTITUTE OF TECHNOLOGY

    E-Print Network [OSTI]

    TOKYO INSTITUTE OF TECHNOLOGY 2005 TOKYO INSTITUTE OF TECHNOLOGY 152-8550 2 12 1 E3-3 2005 8 TEL. 03 5734 2975 URL. http://www.titech.ac.jp/ PROFILE #12;TOKYO INSTITUTE OF TECHNOLOGY 0201 CONTENTS 03 06 06 08 09 10 15 17 25 31 33 37 41 0201 #12;TOKYO INSTITUTE OF TECHNOLOGY TOKYO INSTITUTE

  14. Predictive Maintenance Technologies

    Broader source: Energy.gov [DOE]

    Several diagnostic technologies and best practices are available to assist Federal agencies with predictive maintenance programs.

  15. Hydrogen delivery technology roadmap

    SciTech Connect (OSTI)

    None, None

    2005-11-15

    Document describing plan for research into and development of hydrogen delivery technology for transportation applications.

  16. Northwest Regional Technology Center

    E-Print Network [OSTI]

    management and public safety professionals to define and prioritize technology needs. Coordinate and leadNorthwest Regional Technology Center for Homeland Security The Northwest Regional Technology Center and deployment of technologies that are effective homeland security solutions for the region, and accelerate

  17. SPACE TECHNOLOGY Actual Estimate

    E-Print Network [OSTI]

    technology readiness of new missions, mitigate their technological risks, improve the quality of cost estimates, and thereby contribute to better overall mission cost management..." Space Technology investmentsSPACE TECHNOLOGY TECH-1 Actual Estimate Budget Authority (in $ millions) FY 2011 FY 2012 FY 2013 FY

  18. DNA nanotechnology: understanding and optimisation through simulation

    E-Print Network [OSTI]

    Thomas E. Ouldridge

    2014-11-07

    DNA nanotechnology promises to provide controllable self-assembly on the nanoscale, allowing for the design of static structures, dynamic machines and computational architectures. In this article I review the state-of-the art of DNA nanotechnology, highlighting the need for a more detailed understanding of the key processes, both in terms of theoretical modelling and experimental characterisation. I then consider coarse-grained models of DNA, mesoscale descriptions that have the potential to provide great insight into the operation of DNA nanotechnology if they are well designed. In particular, I discuss a number of nanotechnological systems that have been studied with oxDNA, a recently developed coarse-grained model, highlighting the subtle interplay of kinetic, thermodynamic and mechanical factors that can determine behaviour. Finally, new results highlighting the importance of mechanical tension in the operation of a two-footed walker are presented, demonstrating that recovery from an unintended `overstepped' configuration can be accelerated by three to four orders of magnitude by application of a moderate tension to the walker's track. More generally, the walker illustrates the possibility of biasing strand-displacement processes to affect the overall rate.

  19. Vehicle Technologies Office: 2008 Advanced Vehicle Technology...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Advanced Vehicle Technology Analysis and Evaluation Activities and Heavy Vehicle Systems Optimization Program Annual Progress Report Well-to-Wheels Analysis of Energy Use and...

  20. Vehicle Technologies Office: 2009 Advanced Vehicle Technology...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Advanced Vehicle Technology Analysis and Evaluation Activities and Heavy Vehicle Systems Optimization Program Annual Progress Report 2009avtaehvso.pdf More Documents &...

  1. Conditions for positioning of nucleosomes on DNA

    E-Print Network [OSTI]

    Michael Sheinman; Ho-Ryun Chung

    2015-04-29

    Positioning of nucleosomes along eukaryotic genomes plays an important role in their organization and regulation. There are many different factors affecting the location of nucleosomes. Some can be viewed as preferential binding of a single nucleosome to different locations along the DNA and some as interactions between neighboring nucleosomes. In this study we analyzed how well nucleosomes are positioned along the DNA as a function of strength of the preferential binding, correlation length of the binding energy landscape, interactions between neighboring nucleosomes and others relevant system properties. We analyze different scenarios: designed energy landscapes and generically disordered ones and derive conditions for good positioning. Using analytic and numerical approaches we find that, even if the binding preferences are very weak, synergistic interplay between the interactions and the binding preferences is essential for a good positioning of nucleosomes, especially on correlated energy landscapes. Analyzing empirical energy landscape, we discuss relevance of our theoretical results to positioning of nucleosomes on DNA \\emph{in vivo.}

  2. Flow cytometric measurement of total DNA and incorporated halodeoxyuridine

    DOE Patents [OSTI]

    Dolbeare, Frank A. (Livermore, CA); Gray, Joe W. (Livermore, CA)

    1986-01-01

    A method for the simultaneous flow cytometric measurement of the total DNA content and the level of DNA synthesis in normal and malignant cells is disclosed. The sensitivity of the method allows a study of cell cycle traverse rates for large scale cell populations as well as single cell measurements. A DNA stain such as propidium iodide is used as the probe for the measurement of total DNA content and a monoclonal antibody reactive with a DNA precursor such as bromodeoxyuridine (BrdU) is used as a probe for the measurement of BrdU uptake by the cells as a measure of DNA synthesis.

  3. DNA analysis conference in Santa Fe

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home Room NewsInformation Current HAB Packet Hanford Advisory Board6/23/2014DLFM libraryDNA OrigamiDNA

  4. Next generation sequencing (NGS)technologies and applications

    SciTech Connect (OSTI)

    Vuyisich, Momchilo

    2012-09-11

    NGS technology overview: (1) NGS library preparation - Nucleic acids extraction, Sample quality control, RNA conversion to cDNA, Addition of sequencing adapters, Quality control of library; (2) Sequencing - Clonal amplification of library fragments, (except PacBio), Sequencing by synthesis, Data output (reads and quality); and (3) Data analysis - Read mapping, Genome assembly, Gene expression, Operon structure, sRNA discovery, and Epigenetic analyses.

  5. Assessing Software Engineering Technology Transfer

    E-Print Network [OSTI]

    Zelkowitz, Marvin V.

    , and technology infusion, or the adoption of a new technology by an individual organization. 1 #12;Table ¢ ¡ £ ¡ ¢ ¡ ¡ ¢ ¡ ¡ ¡ ¢ ¡ £ ¤ £ ¡ ¡ ¢ ¡ ¡ ¢ ¡ ¡ £ ¤ £ ¡ ¢ ¡ ¡ ¡ ¢ ¡ ¡ ¢ ¡ £ ¡ ¢ 15 3.4 Exporting and Infusing Technology ¡ ¡ ¡ ¢ ¡ £ ¤ £ ¡ ¡ ¢ ¡ ¡ ¢ ¡ ¡ £ ¤ £ ¡ ¢ ¡ ¡ ¡ ¢ ¡ ¡ ¢ ¡ £ ¡ ¢ 16 4 Infusion of Technology 18 4.1 Technologies of Interest

  6. SHARED TECHNOLOGY TRANSFER PROGRAM

    SciTech Connect (OSTI)

    GRIFFIN, JOHN M. HAUT, RICHARD C.

    2008-03-07

    The program established a collaborative process with domestic industries for the purpose of sharing Navy-developed technology. Private sector businesses were educated so as to increase their awareness of the vast amount of technologies that are available, with an initial focus on technology applications that are related to the Hydrogen, Fuel Cells and Infrastructure Technologies (Hydrogen) Program of the U.S. Department of Energy. Specifically, the project worked to increase industry awareness of the vast technology resources available to them that have been developed with taxpayer funding. NAVSEA-Carderock and the Houston Advanced Research Center teamed with Nicholls State University to catalog NAVSEA-Carderock unclassified technologies, rated the level of readiness of the technologies and established a web based catalog of the technologies. In particular, the catalog contains technology descriptions, including testing summaries and overviews of related presentations.

  7. Training & Technology Solutions Queens College ~ Office of Converging Technologies ~ Training & Technology Solutions

    E-Print Network [OSTI]

    Johnson Jr.,, Ray

    Training & Technology Solutions Queens College ~ Office of Converging Technologies ~ Training & Technology Solutions 718-997-4875 ~ training@qc.cuny.edu ~ I-Bldg 214 Advisor Center Navigation: Login #12;Training & Technology Solutions Queens College ~ Office of Converging Technologies ~ Training

  8. Analysis of the structural changes caused by positive DNA supercoiling

    E-Print Network [OSTI]

    Barth, Marita Christine

    2007-01-01

    The procession of helix-tracking enzymes along a DNA molecule results in the formation of supercoils in the DNA, with positive supercoiling (overwinding) generated ahead of the enzyme, and negative supercoiling (underwinding) ...

  9. Engineering of DNA-mediated assemblies for biosensing applications /

    E-Print Network [OSTI]

    Xu, Phyllis F.

    2013-01-01

    OF THE DISSERTATION Engineering of DNA-mediated assembliesCALIFORNIA, SAN DIEGO Engineering of DNA-mediated assembliesMaterials Science and Engineering by Phyllis F. Xu Committee

  10. Manipulation of cellular DNA repair by early adenovirus proteins

    E-Print Network [OSTI]

    Orazio, Nicole Ise

    2010-01-01

    The function of BLM in the DDR and in processing of DNA endscellular DNA damage response (DDR) functions to inhibit thespecific components of the DDR, and provides insight into

  11. DNA hybridization : fundamental studies and applications in directed assembly

    E-Print Network [OSTI]

    Bajaj, Manish G. (Manish Gopal)

    2005-01-01

    Programmed self-assembly using non-covalent DNA-DNA interactions is a promising technique for the creation of next-generation functional devices for electronic, optical, and magnetic applications. This thesis develops the ...

  12. Genome scanning : an AFM-based DNA sequencing technique

    E-Print Network [OSTI]

    Elmouelhi, Ahmed (Ahmed M.), 1979-

    2003-01-01

    Genome Scanning is a powerful new technique for DNA sequencing. The method presented in this thesis uses an atomic force microscope with a functionalized cantilever tip to sequence single stranded DNA immobilized to a mica ...

  13. Defining functional DNA elements in the human genome

    E-Print Network [OSTI]

    Kellis, Manolis

    With the completion of the human genome sequence, attention turned to identifying and annotating its functional DNA elements. As a complement to genetic and comparative genomics approaches, the Encyclopedia of DNA Elements ...

  14. THE NEW YORK BOTANICAL GARDEN DNA BANK ACQUISITIONS POLICY

    E-Print Network [OSTI]

    Law, Wayne

    THE NEW YORK BOTANICAL GARDEN DNA BANK ACQUISITIONS POLICY The DNA Bank of The New York Botanical herbarium. Please contact Lisa M. Campbell, Plant Research Laboratory, The New York Botanical Garden, Bronx

  15. Unveiling Stability Criteria of DNA-Carbon Nanotubes Constructs...

    Office of Scientific and Technical Information (OSTI)

    constructs. Application of STM allows direct observation of very stable CNT-DNA hybrid structures with the well-defined DNA wrapping angle of 63.4and a coiling period of...

  16. Dna electrophoresis in photopolymerized polyacrylamide gels on a microfluidic device 

    E-Print Network [OSTI]

    Lo, Chih-Cheng

    2009-05-15

    -throughput DNA gel electrophoresis. However, further progress toward dramatic improvements of separation performance over ultra-short distances requires a much more detailed understanding of the physics of DNA migration in the sieving gel matrix than is currently...

  17. Enhancement of in vitro Translation by Gold Nanoparticle – DNA Conjugates

    E-Print Network [OSTI]

    Park, Sunho

    Gold nanoparticle (AuNP)?DNA conjugates can enhance in vitro translation of a protein. Enhancement occurs via a combination of nonspecific adsorption of translation-related molecules and the ribosome to the AuNP?DNA and ...

  18. Role of DNA repair protein ERCC1 in skin cancer 

    E-Print Network [OSTI]

    Song, Liang

    2009-01-01

    Nucleotide excision repair (NER) is one of the major repair systems for removal of DNA lesions. The NER pathway has evolved mainly to repair UV-induced DNA damage and is also active against a broad range of endogenously ...

  19. A model for sample stacking in microcapillary DNA electrophoresis

    E-Print Network [OSTI]

    Srivastava, Alok Kumar, 1967-

    2002-01-01

    Sanger's method of chain termination is the method of choice in DNA sequencing, where electrophoresis is used to separate the different sized DNA. In the past decade, microfabricated capillary devices have been developed ...

  20. Flow cytometric measurement of total DNA and incorporated halodeoxyuridine

    DOE Patents [OSTI]

    Dolbeare, F.A.; Gray, J.W.

    1983-10-18

    A method for the simultaneous flow cylometric measurement of total cellular DNA content and of the uptake of DNA precursors as a measure of DNA synthesis during various phases of the cell cycle in normal and malignant cells in vitro and in vivo is described. The method comprises reacting cells with labelled halodeoxyuridine (HdU), partially denaturing cellular DNA, adding to the reaction medium monoclonal antibodies (mabs) reactive with HdU, reacting the bound mabs with a second labelled antibody, incubating the mixture with a DNA stain, and measuring simultaneously the intensity of the DNA stain as a measure of the total cellular DNA and the HdU incorporated as a measure of DNA synthesis. (ACR)

  1. Photoelectrochemical array platform for genomic scale DNA synthesis

    E-Print Network [OSTI]

    Emig, Christopher Joseph

    2006-01-01

    Molecular and synthetic biologists have increasing demand for large, high-fidelity constructs of synthetic DNA. Recent developments in harvesting oligonucleotides from DNA microarrays has proven that these can be assembled ...

  2. Single cell trapping and DNA damage analysis using microwell arrays

    E-Print Network [OSTI]

    Wood, David

    With a direct link to cancer, aging, and heritable diseases as well as a critical role in cancer treatment, the importance of DNA damage is well-established. The intense interest in DNA damage in applications ranging from ...

  3. Alternative Methods for Human Identification: Mitochondrial DNA Base Composition Profiling

    E-Print Network [OSTI]

    Applied Genetics Alternative Methods for Human Identification: Mitochondrial DNA Base Composition · Evaluation Experiments · Future directions #12;Applied Genetics Mitochondrial DNA · Mitochondria are organelles within cells ­ Produce energy via the Krebs Cycle · Separate genome from the nucleus ­ 16,569 bp

  4. The Genetic Structure of the Kuwaiti Population: Mitochondrial DNA Markers

    E-Print Network [OSTI]

    Theyab, Jasem

    2010-06-14

    the expansion of early Homo sapiens out of Africa. Kuwait is located in the Northeast portion of the Arabian Peninsula. This thesis investigated the mitochondrial DNA (mtDNA) genetic variation in 117 unrelated individuals to determine the genetic structure...

  5. Lectin cDNA and transgenic plants derived therefrom

    DOE Patents [OSTI]

    Raikhel, Natasha V. (Okemos, MI)

    2000-10-03

    Transgenic plants containing cDNA encoding Gramineae lectin are described. The plants preferably contain cDNA coding for barley lectin and store the lectin in the leaves. The transgenic plants, particularly the leaves exhibit insecticidal and fungicidal properties.

  6. Isolation of Yeast DNA Prepare in advance

    E-Print Network [OSTI]

    Aris, John P.

    Isolation of Yeast DNA Prepare in advance: · 2.0 ml microfuge tubes containing 0.25 g of 0.5 mm600 ~1, or 1 ml saturated YPD culture at OD600 ~10). Centrifuge for 5 minutes at 2000 rpm. Discard will thaw during centrifugation. Transfer supernatant (200-225 µl) to a fresh 1.5 ml tube. Avoid

  7. Rewritable Memory by Controllable Nanopatterning of DNA

    E-Print Network [OSTI]

    Pierce, Niles A.

    ABSTRACT Fabricating a nanostructure capable of reversibly patterning molecules is a fundamental goal within nanotechnology, underlying diverse processes such as information storage, scaffold functioning of the device as rewritable memory. The bit state of each address is controlled by specific DNA

  8. Advanced Review Mediators and dynamics of DNA

    E-Print Network [OSTI]

    Wang, Wei

    -wide classifies cell types uniquely and in several cases discriminates between healthy and cancerous cell types zinc fin- ger proteins, recognize the presence of methylated cytosines.4­13 DNMT1 maintains methylation mechanism that prevents the activation of these sequences.31­34 DNA methyla- tion is also present in genes

  9. Identification of animal images based on DNA

    E-Print Network [OSTI]

    Wolf, Lior

    ongus ? Given mtDNA, the algorithm identifies the correct unseen image #12;Data sets Fishes of Australia Regularized CCA #12;Results · 93 fish species, 82 for training, 11 for testing · Significantly better than chance or NN #12;Results Fish: 90% correct Birds: 72% correct Dorsal: 59% correct Head: 56% correct

  10. Conserved Steps in Eukaryotic DNA Replication

    E-Print Network [OSTI]

    Blow, J. Julian

    and 50 car- bons of deoxyribose. The 10 carbon of the deoxyribose is linked to one of four different of DNA contains all the information necessary to produce new second strands through complementary base the input of chemical energy to break the hydrogen bonds. This energy is derived from hydrolysis of ATP

  11. A User's Guide to the Encyclopedia of DNA Elements (ENCODE)

    E-Print Network [OSTI]

    Bernstein, Bradley E.

    The mission of the Encyclopedia of DNA Elements (ENCODE) Project is to enable the scientific and medical communities to

  12. Structural basis for the inhibition of human alkyladenine DNA by 3,N4-ethenocytosine containing DNA

    E-Print Network [OSTI]

    Lingaraju, Gondichatnahalli M.

    Reactive oxygen and nitrogen species, generated by neutrophils and macrophages in chronically inflamed tissues, readily damage DNA, producing a variety of potentially genotoxic etheno base lesions; such inflammation-related ...

  13. Validation of DNA probes for molecular cytogenetics by mapping onto immobilized circular DNA

    E-Print Network [OSTI]

    Greulich-Bode, Karin

    2009-01-01

    two linked P1 clones (‘3012’, ‘3015’) that contain one orcircular DNA molecule (‘3012’) as well as the RGB image (J)overlap between clones ‘3012’ and ‘3015’ and the location of

  14. RhoJ Regulates Melanoma Chemoresistance by Suppressing Pathways that Sense DNA Damage

    E-Print Network [OSTI]

    Ho, Hsiang; Aruri, Jayavani; Kapadia, Rubina; Mehr, Hootan; White, Michael A.; Ganesan, Anand K.

    2012-01-01

    Pathways that Sense DNA Damage $watermark-text Hsiang Ho 1 ,16. Roos WP, Kaina B. DNA damage-induced apoptosis: FromDNA lesions to the DNA damage response and apoptosis. Cancer

  15. An Investigation on Gel Electrophoresis with Quantum Dots End-labeled DNA 

    E-Print Network [OSTI]

    Chen, Xiaojia

    2009-05-15

    explored manipulating DNA fragments by end labeling DNA molecules with quantum dot nanocrystals. The quantum dot-DNA conjugates can be further modified through binding interactions with biotinylated single-stranded DNA primers. Single molecule visualization...

  16. UNIVERSITY of STRATHCLYDE TECHNOLOGY &

    E-Print Network [OSTI]

    Mottram, Nigel

    electricity networks and distribution systems, through to using smart grid technologies for more effective of dynamic collaborations delivering productive outcomes. #12;#12;LOW CARBON POWER AND ENERGY FUTURE CITIES Advanced Manufacturing Future Cities Health Technologies Working collaboratively, programmes within

  17. Massachusetts Institute of Technology

    E-Print Network [OSTI]

    ChemE Massachusetts Institute of Technology Department of Chemical Engineering Undergraduate technology, chemical engineers play a role in almost every industry and they collaborate with all types, creating and improving pharmaceuticals, fuels, polymers, plastics, cosmetics, cereals and more." Klavs

  18. SIMULATING EVOLUTION OF TECHNOLOGY

    E-Print Network [OSTI]

    SIMULATING EVOLUTION OF TECHNOLOGY: AN AID TO ENERGY POLICY ANALYSIS A CASE STUDY OF STRATEGIES Approval Name: John Nyboer Degree: Doctor of Philosophy Title of Thesis: Simulating Evolution of Technology

  19. Technology Readiness Assessment Guide

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2011-09-15

    The Guide assists individuals and teams involved in conducting Technology Readiness Assessments (TRAs) and developing Technology Maturation Plans (TMPs) for the DOE capital asset projects subject to DOE O 413.3B. Supersedes DOE G 413.3-4.

  20. Technology Deployment Case Studies

    Broader source: Energy.gov [DOE]

    Find technology deployment case studies below. Click on each individual project link to see the full case study. You can also view a map of technology deployment case studies.

  1. Utilities Inspection Technologies 

    E-Print Network [OSTI]

    Messock, R. K.

    1993-01-01

    Preventive and predictive maintenance programs are enhanced by using various inspection technologies to detect problems and potential failures before catastrophic failure. This paper discusses successful inspection technologies that have been...

  2. Technology Business Incubation Programme

    E-Print Network [OSTI]

    1 Technology Business Incubation Programme Ms. Kimmie Wong Assistant Manager Incubation Admission and organization. Industry Technology Biotechnology Clusters IT & Telecomm. Pharmaceutical Precision Engg. Chinese Lab Premises Technical Support Facilities Technical and Management Assistance Management and Technical

  3. Tag: technology transfer

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    17all en CNS, UT chemical sensing technology wins R&D 100 Award http:www.y12.doe.govnewspress-releasescns-ut-chemical-sensing-technology-wins-rd-100-award

  4. Protein search for multiple targets on DNA

    E-Print Network [OSTI]

    Martin Lange; Maria Kochugaeva; Anatoly B. Kolomeisky

    2015-08-03

    Protein-DNA interactions are crucial for all biological processes. One of the most important fundamental aspects of these interactions is the process of protein searching and recognizing specific binding sites on DNA. A large number of experimental and theoretical investigations have been devoted to uncovering the molecular description of these phenomena, but many aspects of the mechanisms of protein search for the targets on DNA remain not well understood. One of the most intriguing problems is the role of multiple targets in protein search dynamics. Using a recently developed theoretical framework we analyze this question in detail. Our method is based on a discrete-state stochastic approach that takes into account most relevant physical-chemical processes and leads to fully analytical description of all dynamic properties. Specifically, systems with two and three targets have been explicitly investigated. It is found that multiple targets in most cases accelerate the search in comparison with a single target situation. However, the acceleration is not always proportional to the number of targets. Surprisingly, there are even situations when it takes longer to find one of the multiple targets in comparison with the single target. It depends on the spatial position of the targets, distances between them, average scanning lengths of protein molecules on DNA, and the total DNA lengths. Physical-chemical explanations of observed results are presented. Our predictions are compared with experimental observations as well as with results from a continuum theory for the protein search. Extensive Monte Carlo computer simulations fully support our theoretical calculations.

  5. Generation of Full-Length cDNA Library

    E-Print Network [OSTI]

    Chuong, Cheng-Ming

    Generation of Full- Length cDNA Library from Single Human Prostate Cancer Cells BioTechniques 27 are performed on fixed and per- meabilized cells. Subsequent RT-PCR generates full-length cDNA libraries. Flowchart of current method for generating a full-length cDNA library from single cells. Cell fixation

  6. Mechanochemistry of a Viral DNA Packaging Motor , Jeffrey Moffitt1

    E-Print Network [OSTI]

    Oster, George

    Mechanochemistry of a Viral DNA Packaging Motor Jin Yu1 , Jeffrey Moffitt1 , Craig L. Hetherington1 The pentameric ATPase motor gp16 packages double-stranded DNA into the bacteriophage 29 virus capsid to explain how the packaging motor translocates the DNA in bursts of four 2.5 bp power strokes, while

  7. Footprinting proteinDNA complexes using the hydroxyl radical

    E-Print Network [OSTI]

    Tullius, Thomas D.

    Footprinting protein­DNA complexes using the hydroxyl radical Swapan S Jain & Thomas D Tullius.2008.72 Hydroxyl radical footprinting has been widely used for studying the structure of DNA and DNA­protein complexes. The high reactivity and lack of base specificity of the hydroxyl radical makes it an excellent

  8. Single-DNA Molecule Nanomotor Regulated by Photons

    E-Print Network [OSTI]

    Tan, Weihong

    Single-DNA Molecule Nanomotor Regulated by Photons Huaizhi Kang, Haipeng Liu, Joseph A. Phillips the design of a single-molecule nanomotor driven by photons. The nanomotor is a DNA hairpin photonic wires,8 enzyme assemblies,9 and functional DNA probes.10,11 Similar to RNA or protein

  9. Advances in DNA Testing for Genealogy and Anthropology

    E-Print Network [OSTI]

    Aazhang, Behnaam

    Advances in DNA Testing for Genealogy and Anthropology Join the MIT Enterprise Forum of Texas that are affecting Genealogy, Anthropology, Medicine, and more. In the second half of the program, Russ Capper company in the world for Genealogy (Family Tree DNA). Family Tree DNA has collaborated with other

  10. Carbon Fiber Technology Facility

    Broader source: Energy.gov [DOE]

    2013 DOE Hydrogen and Fuel Cells Program and Vehicle Technologies Program Annual Merit Review and Peer Evaluation Meeting

  11. Membrane Technology Workshop

    Broader source: Energy.gov [DOE]

    Presentation by Charles Page (Air Products & Chemicals, Inc.) for the Membrane Technology Workshop held July 24, 2012

  12. States & Emerging Energy Technologies

    Office of Energy Efficiency and Renewable Energy (EERE)

    This presentation, given through the DOE's Technical Assitance Program (TAP), provides information on States & Emerging Energy Technologies.

  13. Technology in water conservation 

    E-Print Network [OSTI]

    Finch, Dr. Calvin

    2013-01-01

    ?? percent to ?? percent. Water reuse systems treat wastewater by various technologies including ?ltering, bioremediation and ozone exposure. ?ese technologies can involve billions of gallons of wastewater ? such as in a municipal recycling e... Column by Dr. Calvin Finch, Water Conservation and Technology Center director WAT E R CONSERVATION & TECHNOLOGY CENTER Securing Our Water Future It is not unusual for individuals to describe water conservation as a behavioral exercise and urge...

  14. Advanced Propulsion Technology Strategy

    Broader source: Energy.gov [DOE]

    GM is also developing new classes of electrically driven vehicles, leveraging technology first used in their hybrids.

  15. Do New Technologies Matter?

    Office of Energy Efficiency and Renewable Energy (EERE)

    Check out a few stories of companies who have taken a breakthrough energy technology and run with it.

  16. Consumer Vehicle Technology Data

    Broader source: Energy.gov [DOE]

    2013 DOE Hydrogen and Fuel Cells Program and Vehicle Technologies Program Annual Merit Review and Peer Evaluation Meeting

  17. Technology Readiness Assessment (TRA)/Technology Maturation Plan...

    Energy Savers [EERE]

    (TRA)Technology Maturation Plan (TMP) Process Guide Technology Readiness Assessment (TRA)Technology Maturation Plan (TMP) Process Guide This document is a guide for those...

  18. Vehicle Technologies Office: 2010 Fuel Technologies R&D Annual...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Vehicle Technologies Office: 2010 Fuel Technologies R&D Annual Progress Report The Fuels Technologies subprogram supports fuels and lubricants research and development (R&D)...

  19. EM Engineering & Technology Roadmap and Major Technology Demonstration...

    Office of Environmental Management (EM)

    Processing Office of Engineering and Technology April 2008 EM Engineering & Technology Roadmap and Major Technology Demonstrations Introduction Progress made in EM cleanup...

  20. Vehicle Technologies Office: 2012 Fuel and Lubricant Technologies...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    2 Fuel and Lubricant Technologies R&D Annual Progress Report Vehicle Technologies Office: 2012 Fuel and Lubricant Technologies R&D Annual Progress Report The Fuel & Lubricant...

  1. Does Doctrine Drive Technology or Does Technology Drive Doctrine?

    E-Print Network [OSTI]

    Blasko, Dennis

    2010-01-01

    Policy Brief No. 4 September 2010 Does Doctrine DriveTechnology or Does Technology Drive Doctrine? Dennis Blaskoone way. However, technology does not determine strat- egy.

  2. Does Doctrine Drive Technology or Does Technology Drive Doctrine?

    E-Print Network [OSTI]

    Blasko, Dennis

    2010-01-01

    Brief No. 4 September 2010 Does Doctrine Drive Technology orDoes Technology Drive Doctrine? Dennis Blasko Summary Wthat emphasizes strategy over technology and may hold some

  3. MHK Technologies/Oregon State University Columbia Power Technologies...

    Open Energy Info (EERE)

    OSU Project(s) where this technology is utilized *MHK ProjectsOSU Direct Drive Power Generation Buoys Technology Resource Click here Wave Technology Type Click here Point...

  4. Vehicle Technologies Office: 2008 Oak Ridge Transportation Technology...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Oak Ridge Transportation Technology Program Annual Report Vehicle Technologies Office: 2008 Oak Ridge Transportation Technology Program Annual Report ornlttpreportfy08.pdf More...

  5. Technology Innovations and Experience Curves for Nitrogen Oxides Control Technologies

    E-Print Network [OSTI]

    Yeh, Sonia; Rubin, Edward S.; Taylor, Margaret R.

    2007-01-01

    including issues of technology and cost un- certainties, areon NO x Control Technologies and Cost Effectiveness forand other factors on technology cost trends (hence, the

  6. Technology Advertising Contact Information

    E-Print Network [OSTI]

    Peters, Richard

    Overview #12;Technology Advertising Contact Information Alex Sheath 8596 4063 asheath Overview Our online Technology section is geared towards an IT professional environment, reaching a range of technology enthusiasts from every day gadget consumers to business decision makers where enterprise solutions

  7. Department of Science, Technology, &

    E-Print Network [OSTI]

    Huang, Wei

    Developing Leaders of Innovation Department of Science, Technology, & Society #12;Understanding the relationship between technology and society is crucial to becoming a successful leader in any field. #12;Our Students The University of Virginia Department of Science, Technology, and Society offers a comprehensive

  8. Responder Technology Alert Monthly

    E-Print Network [OSTI]

    PNNL-24014 Responder Technology Alert Monthly (Oct-Nov 2014) January 2015 JF Upton SL Stein #12;#12;PNNL-24014 Responder Technology Alert Monthly (Oct-Nov 2014) JF Upton SL Stein January 2015 Prepared for the Department of Homeland Security Science and Technology Directorate under Contract HSHQPM-14-X-00058. Pacific

  9. PUBLICATIONS BRANCH OF TECHNOLOGY

    E-Print Network [OSTI]

    INDEX of PUBLICATIONS by the BRANCH OF TECHNOLOGY BUREAU OF COMMERCIAL FISHERIES, 1955-59 Inclusive OF PUBLICATIONS BY THE BRANCH OF TECHNOLOGY BUREAU OF COMMERCIAL FISHERIES 1955-59 Inclusive by F. Bruce Sanford continue s, for the year s 1955- 59, the listing of publications by the Branch of Technology given

  10. Science &Technology Facilities Council

    E-Print Network [OSTI]

    Science &Technology Facilities Council Accelerator Science and Technology Centre Daresbury Science)1235 445808 www.stfc.ac.uk/astec Head office, Science and Technology Facilities Council, Polaris House, North Newton Group, La Palma: Joint Astronomy Centre, Hawaii. ASTeC Science Highlights 2009 - 2010 Science

  11. Technology Performance Exchange

    SciTech Connect (OSTI)

    2015-09-01

    To address the need for accessible, high-quality data, the Department of Energy has developed the Technology Performance Exchange (TPEx). TPEx enables technology suppliers, third-party testing laboratories, and other entities to share product performance data. These data are automatically transformed into a format that technology evaluators can easily use in their energy modeling assessments to inform procurement decisions.

  12. NORTHWEST REGIONAL TECHNOLOGY CENTER

    E-Print Network [OSTI]

    NORTHWEST REGIONAL TECHNOLOGY CENTER for Homeland Security Northwest Regional Technology Center May 2015 | 1 AROUND THE REGION IN HOMELAND SECURITY The Northwest Regional Technology Center (NWRTC.S. Army Cyber Command; and Michael Echols, Director, Cyber Joint Program Management Office National

  13. Web Technology (elective package)

    E-Print Network [OSTI]

    Franssen, Michael

    Web Technology (elective package) Offered by: Department of Mathematics and Computer Science? Computer Science-based approaches and enabling technologies for the web. Course descriptions Human and efficient. Web Technology The web has become the major source of information retrieval and is playing

  14. Transmission Enhancement Technology Report

    E-Print Network [OSTI]

    a recommendation of the most cost-effective methods and technologies to enhance electricity transmission from a review of methods and technologies with potential to enhance electricity transmission capability-traditional methods and technologies to increase the capacity of the high voltage electric power transmission system

  15. Can we model DNA at the mesoscale ? Comment on: Fluctuations in the DNA double helix: A critical review

    E-Print Network [OSTI]

    Peyrard, Michel

    2015-01-01

    Comment on "Fluctuations in the DNA double helix: A critical review" by Frank-Kamenetskii and Prakash

  16. http://blogs.ucdavis.edu/egghead/[9/1/2010 1:48:11 PM] DNA repair machine on the bench

    E-Print Network [OSTI]

    Kowalczykowski, Stephen C.

    Egghead http://blogs.ucdavis.edu/egghead/[9/1/2010 1:48:11 PM] DNA repair machine on the bench of Technology. Funding was provided by the National Institutes of Health and the Swiss National Science with social bookmarking: #12;Egghead http://blogs.ucdavis.edu/egghead/[9/1/2010 1:48:11 PM] #12;

  17. The 125 MW Upper Mahiao geothermal power plant

    SciTech Connect (OSTI)

    Forte, N.

    1996-12-31

    The 125 MW Upper Mahiao power plant, the first geothermal power project to be financed under a Build-Own-Operate-and-Transfer (BOOT) arrangement in the Philippines, expected to complete its start-up testing in August of this year. This plant uses Ormat`s environmentally benign technology and is both the largest geothermal steam/binary combined cycle plant as well as the largest geothermal power plant utilizing air cooled condensers. The Ormat designed and constructed plant was developed under a fast track program, with some two years from the April 1994 contract signing through design, engineering, construction and startup. The plant is owned and operated by a subsidiary of CalEnergy Co., Inc. and supplies power to PNOC-Energy Development Corporation for the National Power Corporation (Napocor) national power grid in the Philippines.

  18. Building Technologies Office Window and Envelope Technologies...

    Broader source: Energy.gov (indexed) [DOE]

    Technologies R&D Program Karma Sawyer, Ph.D. karma.sawyer@ee.doe.gov BTO Goal Reduce building energy use by 50% in 2030, compared to the "business-as- usual" energy consumption...

  19. DNA digestion protocol & hints Overview: Although it is pretty standard to digest DNA with restriction enzymes, here

    E-Print Network [OSTI]

    Doering, Tamara

    Liu 4/2004 DNA digestion protocol & hints Overview: Although it is pretty standard to digest DNA in molecular biology (3.1.1-3.1.2) Materials: · DNA sample in water or TE buffer · 10x digestion buffer.1 to 4 µg 10x Digestion buffer 2 µl 5 µl Enzyme ? ? Water Rest of volume Rest of volume 2. Add the enzyme

  20. Carrier DNA For Yeast Transformation Preparation of high molecular weight single stranded carrier DNA for yeast transformations.

    E-Print Network [OSTI]

    Aris, John P.

    minute pulses. Chill on ice for 1 minute in between. The DNA solution should not heat above room into ice bucket to cool quickly. Keep on ice. This denaturation step also sterilizes the DNA. 10. Freeze to collect solution at bottom of tube. Place on ice. 3. Sonicate DNA solution in tube with tip sonifier

  1. Technology Investment Roadmap 2012 -2017

    E-Print Network [OSTI]

    Hickman, Mark

    Technology Investment Roadmap 2012 - 2017 20 February 2012 #12;2 Contents Introduction & Overview ............................................................................................ 8 Trend 3: Technology Enabled Learning .................................................................................................... 16 2. Technology enabled learning and teaching

  2. Technology Policy and Economic Growth

    E-Print Network [OSTI]

    Borrus, Michael; Stowsky, Jay

    1997-01-01

    economic growth) and the Pentagon’s Technology Reinvestment20 Tassey, Technology and Economic Growth: Implications forTechnology Policy and Economic Growth Michael Borrus Jay

  3. 2008 Solar Technologies Market Report

    E-Print Network [OSTI]

    Price, S.

    2010-01-01

    Concentrating Solar Power—Technology, Cost, and Markets.Concentrating Solar Power—Technology, Cost, and Markets.Concentrating Solar Power—Technology, Cost, and Markets.

  4. 2011 Wind Technologies Market Report

    E-Print Network [OSTI]

    Bolinger, Mark

    2013-01-01

    land-based wind energy technology. 2011 Wind Technologiesfor a variety of energy technologies, including wind energy.Renewable Energy Laboratory’s National Wind Technology

  5. 2010 Wind Technologies Market Report

    E-Print Network [OSTI]

    Wiser, Ryan

    2012-01-01

    land-based wind energy technology. 2010 Wind Technologiesfor a variety of energy technologies, including wind energy.2010 Wind Technologies Market Report Federal Energy

  6. 2012 Wind Technologies Market Report

    E-Print Network [OSTI]

    Wiser, Ryan

    2014-01-01

    land-based wind energy technology. 2012 Wind Technologiesfor a variety of energy technologies, including wind energy.of Energy (DOE) Wind & Water Power Technology Office team

  7. High Impact Technology Hub- Results

    Broader source: Energy.gov [DOE]

    Highlights, outcomes and activities to support the adoption of High Impact TechnologiesTechnology Highlights preview early results from current technology demonstrations.  Case Studies overview...

  8. Advanced Integrated Systems Technology Development

    E-Print Network [OSTI]

    2013-01-01

    Renewable Energy Technologies Transportation Advanced Integrated Systems Technology Development is the final report for the Advanced Integrated Systems Technology Development project (

  9. Technology Innovation Program | Partnerships | ORNL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Innovation Program SHARE Technology Innovation Program The Technology Innovation Program (TIP) is a 1-year program designed to accelerate selected technologies to commercial...

  10. T4 DNA condensation in water-alcohol media

    E-Print Network [OSTI]

    M. O. Gallyamov; O. A. Pyshkina; V. G. Sergeyev; I. V. Yaminsky

    2011-07-21

    The process of compaction of high molecular weight DNA T4 is investigated directly in a AFM liquid cell. The AFM-images of globules formed by DNA molecules in the result of compaction in water-alcohol environments at high izopropanol concentration (80%) are received; it is found that at intermediate concentration of izopropanol (40-50%) the DNA molecules form partially compacted formations in which the separate coils of macromolecules twist in toroidal structures. It is shown using the technique of deconvolution of the AFM-images that the globule include only one closely packed DNA molecule. The model of DNA packing is proposed on the basis of AFM experiment.

  11. Preparation of DNA-containing extract for PCR amplification

    DOE Patents [OSTI]

    Dunbar, John M.; Kuske, Cheryl R.

    2006-07-11

    Environmental samples typically include impurities that interfere with PCR amplification and DNA quantitation. Samples of soil, river water, and aerosol were taken from the environment and added to an aqueous buffer (with or without detergent). Cells from the sample are lysed, releasing their DNA into the buffer. After removing insoluble cell components, the remaining soluble DNA-containing extract is treated with N-phenacylthiazolium bromide, which causes rapid precipitation of impurities. Centrifugation provides a supernatant that can be used or diluted for PCR amplification of DNA, or further purified. The method may provide a DNA-containing extract sufficiently pure for PCR amplification within 5–10 minutes.

  12. Obama Administration Announces Availability of $3.9 Billion to Invest in

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of Natural GasAdjustmentsShirleyEnergy AEnergy Managing853926 NewsORMAT NEVADA ORMAT NEVADA2016Smart Grid Technologies

  13. Technology reviews: Glazing systems

    SciTech Connect (OSTI)

    Schuman, J.; Rubinstein, F.; Papamichael, K.; Beltran, L.; Lee, E.S.; Selkowitz, S.

    1992-09-01

    We present a representative review of existing, emerging, and future technology options in each of five hardware and systems areas in envelope and lighting technologies: lighting systems, glazing systems, shading systems, daylighting optical systems, and dynamic curtain wall systems. The term technology is used here to describe any design choice for energy efficiency, ranging from individual components to more complex systems to general design strategies. The purpose of this task is to characterize the state of the art in envelope and lighting technologies in order to identify those with promise for advanced integrated systems, with an emphasis on California commercial buildings. For each technology category, the following activities have been attempted to the extent possible: Identify key performance characteristics and criteria for each technology; determine the performance range of available technologies; identify the most promising technologies and promising trends in technology advances; examine market forces and market trends; and develop a continuously growing in-house database to be used throughout the project. A variety of information sources have been used in these technology characterizations, including miscellaneous periodicals, manufacturer catalogs and cut sheets, other research documents, and data from previous computer simulations. We include these different sources in order to best show the type and variety of data available, however publication here does not imply our guarantee of these data. Within each category, several broad classes are identified, and within each class we examine the generic individual technologies that fag into that class.

  14. Inhibition of DNA ejection from bacteriophage by Mg+2 counterions

    E-Print Network [OSTI]

    SeIl Lee; C. V. Tran; T. T. Nguyen

    2010-11-09

    The problem of inhibiting viral DNA ejection from bacteriophages by multivalent counterions, specifically Mg$^{+2}$ counterions, is studied. Experimentally, it is known that MgSO$_4$ salt has a strong and non-monotonic effect on the amount of DNA ejected. There exists an optimal concentration at which the minimum amount of DNA is ejected from the virus. At lower or higher concentrations, more DNA is ejected from the capsid. We propose that this phenomenon is the result of DNA overcharging by Mg$^{+2}$ multivalent counterions. As Mg$^{+2}$ concentration increases from zero, the net charge of DNA changes from negative to positive. The optimal inhibition corresponds to the Mg$^{+2}$ concentration where DNA is neutral. At lower/higher concentrations, DNA genome is charged. It prefers to be in solution to lower its electrostatic self-energy, which consequently leads to an increase in DNA ejection. By fitting our theory to available experimental data, the strength of DNA$-$DNA short range attraction energies, mediated by Mg$^{+2}$, is found to be $-$0.004 $k_BT$ per nucleotide base. This and other fitted parameters agree well with known values from other experiments and computer simulations. The parameters are also in aggreement qualitatively with values for tri- and tetra-valent counterions.

  15. MCM ring hexamerization is a prerequisite for DNA-binding

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Froelich, Clifford A.; Nourse, Amanda; Enemark, Eric J.

    2015-09-13

    The hexameric Minichromosome Maintenance (MCM) protein complex forms a ring that unwinds DNA at the replication fork in eukaryotes and archaea. Our recent crystal structure of an archaeal MCM N-terminal domain bound to single-stranded DNA (ssDNA) revealed ssDNA associating across tight subunit interfaces but not at the loose interfaces, indicating that DNA-binding is governed not only by the DNA-binding residues of the subunits (MCM ssDNA-binding motif, MSSB) but also by the relative orientation of the subunits. We now extend these findings to show that DNA-binding by the MCM N-terminal domain of the archaeal organism Pyrococcus furiosus occurs specifically in themore »hexameric oligomeric form. We show that mutants defective for hexamerization are defective in binding ssDNA despite retaining all the residues observed to interact with ssDNA in the crystal structure. One mutation that exhibits severely defective hexamerization and ssDNA-binding is at a conserved phenylalanine that aligns with the mouse Mcm4(Chaos3) mutation associated with chromosomal instability, cancer, and decreased intersubunit association.« less

  16. Science & Technology RoadmapScience & Technology Roadmap 03/24/20063:03 PMSOCD Science & Technology Roadmap

    E-Print Network [OSTI]

    Kuligowski, Bob

    Science & Technology RoadmapScience & Technology Roadmap #12;03/24/20063:03 PMSOCD Science & Technology Roadmap 2 TABLE OF CONTENTS EXECUTIVE SUMMARY ..........................................................................................................................................19 4 ROADMAPS AND LINKAGES

  17. Distinct kinetics of human DNA ligases I, IIIalpha, IIIbeta, and IV reveal direct DNA sensing ability and differential physiological functions in DNA repair

    SciTech Connect (OSTI)

    Chen, Xi; Ballin, Jeff D.; Della-Maria, Julie; Tsai, Miaw-Sheue; White, Elizabeth J.; Tomkinson, Alan E.; Wilson, Gerald M.

    2009-05-11

    The three human LIG genes encode polypeptides that catalyze phosphodiester bond formation during DNA replication, recombination and repair. While numerous studies have identified protein partners of the human DNA ligases (hLigs), there has been little characterization of the catalytic properties of these enzymes. In this study, we developed and optimized a fluorescence-based DNA ligation assay to characterize the activities of purified hLigs. Although hLigI joins DNA nicks, it has no detectable activity on linear duplex DNA substrates with short, cohesive single-strand ends. By contrast, hLigIII{beta} and the hLigIII{alpha}/XRCC1 and hLigIV/XRCC4 complexes are active on both nicked and linear duplex DNA substrates. Surprisingly, hLigIV/XRCC4, which is a key component of the major non-homologous end joining (NHEJ) pathway, is significantly less active than hLigIII on a linear duplex DNA substrate. Notably, hLigIV/XRCC4 molecules only catalyze a single ligation event in the absence or presence of ATP. The failure to catalyze subsequent ligation events reflects a defect in the enzyme-adenylation step of the next ligation reaction and suggests that, unless there is an in vivo mechanism to reactivate DNA ligase IV/XRCC4 following phosphodiester bond formation, the cellular NHEJ capacity will be determined by the number of adenylated DNA ligaseIV/XRCC4 molecules.

  18. Graz University of Technology Institute for Software Technology

    E-Print Network [OSTI]

    Graz University of Technology Institute for Software Technology Birgit Vogtenhuber Problem Analysis.054, 3 VU Birgit Vogtenhuber Institute for Software Technology Graz University of Technology email: bvogt of Technology Institute for Software Technology Birgit Vogtenhuber Problem Analysis and Complexity Theory, 716

  19. Fast DNA Sequencing via Transverse Electronic Transport

    E-Print Network [OSTI]

    Johan Lagerqvist; Michael Zwolak; Massimiliano Di Ventra

    2006-03-01

    A rapid and low-cost method to sequence DNA would usher in a revolution in medicine. We propose and theoretically show the feasibility of a protocol for sequencing based on the distributions of transverse electrical currents of single-stranded DNA while it translocates through a nanopore. Our estimates, based on the statistics of these distributions, reveal that sequencing of an entire human genome could be done with very high accuracy in a matter of hours without parallelization, e.g., orders of magnitude faster than present techniques. The practical implementation of our approach would represent a substantial advancement in our ability to study, predict and cure diseases from the perspective of the genetic makeup of each individual.

  20. Adam Merkling Major: Information Technology

    E-Print Network [OSTI]

    Hamburger, Peter

    #12;Adam Merkling Major: Information Technology Position: Student System Administrator Employed Technology Position: User Technology Services Student Technician Employed Since: February 2011 #12;Gabi Mosquera Major: Electrical/Computer Engineering Position: User Technology Services Student Technician

  1. Image credit: Dreamstime Technology for

    E-Print Network [OSTI]

    through development of superconducting magnet technology. Without that research today's high- resolutionImage credit: Dreamstime Technology for research saves lives Technology developed to advance STFC in particle physics technology not only supported important experiments at CERN but pioneered early

  2. Technology transfer 1994

    SciTech Connect (OSTI)

    Not Available

    1994-01-01

    This document, Technology Transfer 94, is intended to communicate that there are many opportunities available to US industry and academic institutions to work with DOE and its laboratories and facilities in the vital activity of improving technology transfer to meet national needs. It has seven major sections: Introduction, Technology Transfer Activities, Access to Laboratories and Facilities, Laboratories and Facilities, DOE Office, Technologies, and an Index. Technology Transfer Activities highlights DOE`s recent developments in technology transfer and describes plans for the future. Access to Laboratories and Facilities describes the many avenues for cooperative interaction between DOE laboratories or facilities and industry, academia, and other government agencies. Laboratories and Facilities profiles the DOE laboratories and facilities involved in technology transfer and presents information on their missions, programs, expertise, facilities, and equipment, along with data on whom to contact for additional information on technology transfer. DOE Offices summarizes the major research and development programs within DOE. It also contains information on how to access DOE scientific and technical information. Technologies provides descriptions of some of the new technologies developed at DOE laboratories and facilities.

  3. Determining orientation and direction of DNA sequences

    DOE Patents [OSTI]

    Goodwin, Edwin H. (Los Alamos, NM); Meyne, Julianne (Los Alamos, NM)

    2000-01-01

    Determining orientation and direction of DNA sequences. A method by which fluorescence in situ hybridization can be made strand specific is described. Cell cultures are grown in a medium containing a halogenated nucleotide. The analog is partially incorporated in one DNA strand of each chromatid. This substitution takes place in opposite strands of the two sister chromatids. After staining with the fluorescent DNA-binding dye Hoechst 33258, cells are exposed to long-wavelength ultraviolet light which results in numerous strand nicks. These nicks enable the substituted strand to be denatured and solubilized by heat, treatment with high or low pH aqueous solutions, or by immersing the strands in 2.times.SSC (0.3M NaCl+0.03M sodium citrate), to name three procedures. It is unnecessary to enzymatically digest the strands using Exo III or another exonuclease in order to excise and solubilize nucleotides starting at the sites of the nicks. The denaturing/solubilizing process removes most of the substituted strand while leaving the prereplication strand largely intact. Hybridization of a single-stranded probe of a tandem repeat arranged in a head-to-tail orientation will result in hybridization only to the chromatid with the complementary strand present.

  4. Reactor Technology | Nuclear Science | ORNL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Reactor Technology Advanced Reactor Concepts Advanced Instrumentation & Controls Light Water Reactor Sustainability Safety and Regulatory Technology Small Modular Reactors Nuclear...

  5. Geothermal innovative technologies catalog

    SciTech Connect (OSTI)

    Kenkeremath, D.

    1988-09-01

    The technology items in this report were selected on the basis of technological readiness and applicability to current technology transfer thrusts. The items include technologies that are considered to be within 2 to 3 years of being transferred. While the catalog does not profess to be entirely complete, it does represent an initial attempt at archiving innovative geothermal technologies with ample room for additions as they occur. The catalog itself is divided into five major functional areas: Exploration; Drilling, Well Completion, and Reservoir Production; Materials and Brine Chemistry; Direct Use; and Economics. Within these major divisions are sub-categories identifying specific types of technological advances: Hardware; Software; Data Base; Process/Procedure; Test Facility; and Handbook.

  6. Digital Sensor Technology

    SciTech Connect (OSTI)

    Ted Quinn; Jerry Mauck; Richard Bockhorst; Ken Thomas

    2013-07-01

    The nuclear industry has been slow to incorporate digital sensor technology into nuclear plant designs due to concerns with digital qualification issues. However, the benefits of digital sensor technology for nuclear plant instrumentation are substantial in terms of accuracy, reliability, availability, and maintainability. This report demonstrates these benefits in direct comparisons of digital and analog sensor applications. It also addresses the qualification issues that must be addressed in the application of digital sensor technology.

  7. Overview: STEEL Enabling Technologies

    Broader source: Energy.gov [DOE]

    Presentation from the U.S. DOE Office of Vehicle Technologies "Mega" Merit Review 2008 on February 25, 2008 in Bethesda, Maryland.

  8. 2016 Technology Innovation Projects

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Projects FY 2016 Technology Innovation Project Briefs Demand Response TIP 292: Advanced Heat Pump Water Heater Research TIP 336: Scaled Deployment and Demonstration of Demand...

  9. Overview of biomass technologies

    SciTech Connect (OSTI)

    None, None

    2009-01-18

    The biomass overview of the Renewable Energy Technology Characterizations describes the technical and economic status of this emerging renewable energy option for electricity supply.

  10. Ocean Energy Technology Overview

    SciTech Connect (OSTI)

    none,

    2009-08-05

    Introduction to and overview of ocean renewable energy resources and technologies prepared for the U.S. Department of Energy Federal Energy management Program.

  11. Technology transfer issue

    SciTech Connect (OSTI)

    Jacobson, C.

    1982-05-31

    Testimony by Lawrence J. Brady, Commerce Assistant Secretary for Trade Administration, at Congressional hearings on the national security issues of technology transfers to the Soviet Union identified steps the US needs to take to deal effectively with the problem. These steps include an understanding of how the Soviet Union has and will benefit militarily by acquiring Western technology and efforts to work with other countries, counterintelligence agencies, and industries to stem the flow of technological information. Brady outlined changes in technology development that complicate the enforcement of transfer rules, and emphasized the importance of a close relationship between the business community and the Commerce Department. (DCK)

  12. Supervisory Information Technology Specialist

    Broader source: Energy.gov [DOE]

    A successful candidate in this position will be responsible for providing Information Technology (IT) infrastructure, capabilities and technical support to the Department of Energy (DOE),...

  13. Genome Science/Technologies

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    primary focus is to develop technologies to obtain near complete genomes from single cells for the purposes of improved taxonomic identification and determining metabolic...

  14. Renewable energy technology characterizations

    SciTech Connect (OSTI)

    None, None

    1997-12-01

    The Renewable Energy Technology Characterizations describe the technical and economic status of the major emerging renewable energy options for electricity supply.

  15. TECHNOLOGY PROGRAM PLAN

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    efforts conducted within the Carbon Capture program include development of advanced solvents, sorbents, and membranes for both the Post- and Pre-Combustion Technology Areas...

  16. ENERGY EFFICIENCY TECHNOLOGY ROADMAP

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    managed the overall development and maturation of this Energy Efficiency Technology Roadmap, the effort would not have been possible without the active engagement of a diverse...

  17. Collaborative Transmission Technology Roadmap

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Addendum to the Collaborative Transmission Technology Roadmap March 2014 Bonneville Power Administration Enhanced PDF Functionality Functionality of the PDF version of this...

  18. Mobile Technology Management

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2013-11-21

    The directive will ensure that federal organizations and employees within the Department can use mobile technology to support mission requirements in a safe and secure manner.

  19. Fuel Cell Technologies Budget

    SciTech Connect (OSTI)

    EERE

    2012-03-16

    The Fuel Cell Technologies Office receives appropriations from Energy and Water Development. The offices's major activities and budget are outlined in this Web page.

  20. Arc Position Sensing Technology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    (VAR) furnaces for industries that use specialty metals such as nickel, titanium, and zirconium. The technology could be used to help produce materials with stronger chemical and...

  1. Consumer Vehicle Technology Data

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    technologies Relevance: An informed understanding of the consumer allows VTO to achieve petroleum-use reduction goals through: * Robust assumptions for consumer modeling,...

  2. Consumer Vehicle Technology Data

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    technologies. Relevance: An informed understanding of the consumer allows VTO to achieve petroleum-use reduction goals through: * Robust assumptions for consumer modeling,...

  3. Building Technologies Office

    Broader source: Energy.gov (indexed) [DOE]

    Technologies Office Richard Karney, P.E. May 8, 2014 40% 60% National Energy Consumption Reducing consumption or improving performance calls for cutting-edge...

  4. Marine & Hydrokinetic Technologies

    SciTech Connect (OSTI)

    2011-07-01

    This fact sheet describes the Wind and Water Power Program's current approach to supporting the development and deployment of marine and hydrokinetic technologies.

  5. TEAM Technologies, Inc.

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    that by designating TEAM Technologies as one of only a handful of Strategic Suppliers working at the Laboratories," says Sachs. Licensing Partners - Sandia's Stingray...

  6. Solar Energy Technologies Office

    Office of Energy Efficiency and Renewable Energy (EERE)

    In 2011, the Energy Department's Solar Energy Technologies Office (SETO) became the SunShot Initiative, a collaborative national effort that aggressively drives innovation to make solar energy...

  7. DNA repair decline during mouse spermiogenesis results in the accumulation of heritable DNA damage

    SciTech Connect (OSTI)

    Marchetti, Francesco; Marchetti, Francesco; Wryobek, Andrew J

    2008-02-21

    The post-meiotic phase of mouse spermatogenesis (spermiogenesis) is very sensitive to the genomic effects of environmental mutagens because as male germ cells form mature sperm they progressively lose the ability to repair DNA damage. We hypothesized that repeated exposures to mutagens during this repair-deficient phase result in the accumulation of heritable genomic damage in mouse sperm that leads to chromosomal aberrations in zygotes after fertilization. We used a combination of single or fractionated exposures to diepoxybutane (DEB), a component of tobacco smoke, to investigate how differential DNA repair efficiencies during the three weeks of spermiogenesis affected the accumulation of DEB-induced heritable damage in early spermatids (21-15 days before fertilization, dbf), late spermatids (14-8 dbf) and sperm (7- 1 dbf). Analysis of chromosomalaberrations in zygotic metaphases using PAINT/DAPI showed that late spermatids and sperm are unable to repair DEB-induced DNA damage as demonstrated by significant increases (P<0.001) in the frequencies of zygotes with chromosomal aberrations. Comparisons between single and fractionated exposures suggested that the DNA repair-deficient window during late spermiogenesis may be less than two weeks in the mouse and that during this repair-deficient window there is accumulation of DNA damage in sperm. Finally, the dose-response study in sperm indicated a linear response for both single and repeated exposures. These findings show that the differential DNA repair capacity of post-meioitic male germ cells has a major impact on the risk of paternally transmitted heritable damage and suggest that chronic exposures that may occur in the weeks prior to fertilization because of occupational or lifestyle factors (i.e, smoking) can lead to an accumulation of genetic damage in sperm and result in heritable chromosomal aberrations of paternal origin.

  8. DNA Repair Decline During Mouse Spermiogenesis Results in the Accumulation of Heritable DNA Damage

    SciTech Connect (OSTI)

    Marchetti, Francesco; Marchetti, Francesco; Wyrobek, Andrew J.

    2007-12-01

    The post-meiotic phase of mouse spermatogenesis (spermiogenesis) is very sensitive to the genomic effects of environmental mutagens because as male germ cells form mature sperm they progressively lose the ability to repair DNA damage. We hypothesized that repeated exposures to mutagens during this repair-deficient phase result in the accumulation of heritable genomic damage in mouse sperm that leads to chromosomal aberrations in zygotes after fertilization. We used a combination of single or fractionated exposures to diepoxybutane (DEB), a component of tobacco smoke, to investigate how differential DNA repair efficiencies during the three weeks of spermiogenesis affected the accumulation of DEB-induced heritable damage in early spermatids (21-15 days before fertilization, dbf), late spermatids (14-8 dbf) and sperm (7-1 dbf). Analysis of chromosomal aberrations in zygotic metaphases using PAINT/DAPI showed that late spermatids and sperm are unable to repair DEB-induced DNA damage as demonstrated by significant increases (P<0.001) in the frequencies of zygotes with chromosomal aberrations. Comparisons between single and fractionated exposures suggested that the DNA repair-deficient window during late spermiogenesis may be less than two weeks in the mouse and that during this repair-deficient window there is accumulation of DNA damage in sperm. Finally, the dose-response study in sperm indicated a linear response for both single and repeated exposures. These findings show that the differential DNA repair capacity of post-meioitic male germ cells has a major impact on the risk of paternally transmitted heritable damage and suggest that chronic exposures that may occur in the weeks prior to fertilization because of occupational or lifestyle factors (i.e, smoking) can lead to an accumulation of genetic damage in sperm and result in heritable chromosomal aberrations of paternal origin.

  9. Cytokine secretion profiles in primary cultured cells and in mice stimulated with plasmid DNA

    E-Print Network [OSTI]

    Yoshida, Hiroyuki

    2006-10-27

    University Elimination of DNA by antigen presenting cells Gene therapy Infection Leakage Processing Macrophages and dendritic cells Apoptotic or necrotic cells Endogenous DNA DNA Virus Plasmid DNA Exogenous DNA CpG motif5?- Pur Pur CG Pyr Pyr -3? Danger... signal Naked CpG DNA DNA lipoplex Macrophages or dendritic cells ???? ???? Cytokine geneNF-?B AP-1 ???? ???? Endosome Nucleus ? TLR9 Interferon ?/? gene Inflammatory cytokines TNF-?, IL-6 etc. Type I interferon IFN-?, INF-? ? Mechanisms of immune response...

  10. TOKYO INSTITUTE OF TECHNOLOGY TOKYO INSTITUTE OF TECHNOLOGY TOKYO INSTITUTE OF TECHNOLOGY

    E-Print Network [OSTI]

    #12;06 06 TOKYO INSTITUTE OF TECHNOLOGY 0201 CONTENTS 03 08 09 10 15 17 25 31 33 37 41 0201 08 23 TokyoTech #12;TOKYO INSTITUTE OF TECHNOLOGY TOKYO INSTITUTE OF TECHNOLOGY 0403 20 1 5 11 #12;TOKYO INSTITUTE OF TECHNOLOGY TOKYO INSTITUTE OF TECHNOLOGY 0605 4 3 2 5 5 4 3 #12;TOKYO INSTITUTE OF TECHNOLOGY

  11. Digital Actuator Technology

    SciTech Connect (OSTI)

    Ken Thomas; Ted Quinn; Jerry Mauck; Richard Bockhorst

    2014-09-01

    There are significant developments underway in new types of actuators for power plant active components. Many of these make use of digital technology to provide a wide array of benefits in performance of the actuators and in reduced burden to maintain them. These new product offerings have gained considerable acceptance in use in process plants. In addition, they have been used in conventional power generation very successfully. This technology has been proven to deliver the benefits promised and substantiate the claims of improved performance. The nuclear industry has been reluctant to incorporate digital actuator technology into nuclear plant designs due to concerns due to a number of concerns. These could be summarized as cost, regulatory uncertainty, and a certain comfort factor with legacy analog technology. The replacement opportunity for these types of components represents a decision point for whether to invest in more modern technology that would provide superior operational and maintenance benefits. Yet, the application of digital technology has been problematic for the nuclear industry, due to qualification and regulatory issues. With some notable exceptions, the result has been a continuing reluctance to undertake the risks and uncertainties of implementing digital actuator technology when replacement opportunities present themselves. Rather, utilities would typically prefer to accept the performance limitations of the legacy analog actuator technologies to avoid impacts to project costs and schedules. The purpose of this report is to demonstrate that the benefits of digital actuator technology can be significant in terms of plant performance and that it is worthwhile to address the barriers currently holding back the widespread development and use of this technology. It addresses two important objectives in pursuit of the beneficial use of digital actuator technology for nuclear power plants: 1. To demonstrate the benefits of digital actuator technology over legacy analog sensor technology in both quantitative and qualitative ways. 2. To recognize and address the added difficulty of digital technology qualification, especially in regard to software common cause failure (SCCF), that is introduced by the use of digital actuator technology.

  12. Technology Catalogue. First edition

    SciTech Connect (OSTI)

    Not Available

    1994-02-01

    The Department of Energy`s Office of Environmental Restoration and Waste Management (EM) is responsible for remediating its contaminated sites and managing its waste inventory in a safe and efficient manner. EM`s Office of Technology Development (OTD) supports applied research and demonstration efforts to develop and transfer innovative, cost-effective technologies to its site clean-up and waste management programs within EM`s Office of Environmental Restoration and Office of Waste Management. The purpose of the Technology Catalogue is to provide performance data on OTD-developed technologies to scientists and engineers assessing and recommending technical solutions within the Department`s clean-up and waste management programs, as well as to industry, other federal and state agencies, and the academic community. OTD`s applied research and demonstration activities are conducted in programs referred to as Integrated Demonstrations (IDs) and Integrated Programs (IPs). The IDs test and evaluate.systems, consisting of coupled technologies, at specific sites to address generic problems, such as the sensing, treatment, and disposal of buried waste containers. The IPs support applied research activities in specific applications areas, such as in situ remediation, efficient separations processes, and site characterization. The Technology Catalogue is a means for communicating the status. of the development of these innovative technologies. The FY93 Technology Catalogue features technologies successfully demonstrated in the field through IDs and sufficiently mature to be used in the near-term. Technologies from the following IDs are featured in the FY93 Technology Catalogue: Buried Waste ID (Idaho National Engineering Laboratory, Idaho); Mixed Waste Landfill ID (Sandia National Laboratories, New Mexico); Underground Storage Tank ID (Hanford, Washington); Volatile organic compound (VOC) Arid ID (Richland, Washington); and VOC Non-Arid ID (Savannah River Site, South Carolina).

  13. Emerging Technologies - Capturing Innovation with Technology

    SciTech Connect (OSTI)

    None

    2012-12-01

    ET team research results are critical to achieving 50% energy savings across U.S. buildings within the next two decades. The ET team focuses on supporting research, development, and tech-to-market opportunities of high impact technologies, or those that demonstrate potential for achieving significant energy savings cost effectively.

  14. Capstan friction model for DNA ejection from bacteriophages

    E-Print Network [OSTI]

    Sandip Ghosal

    2013-01-09

    Bacteriophages infect cells by attaching to the outer membrane and injecting their DNA into the cell.The phage DNA is then transcribed by the cell's transcription machinery.A number of physical mechanisms by which DNA can be translocated from the phage capsid into the cell have been identified. A fast ejection driven by the elastic and electrostatic potential energy of the compacted DNA within the viral capsid appears to be used by most phages, at least to initiate infection.In recent in vitro experiments, the speed of DNA translocation from a lambda phage capsid has been measured as a function of ejected length over the entire duration of the event.Here a mechanical model is proposed that is able to explain the observed dependence of exit velocity on ejected length, and that is also consistent with the accepted picture of the geometric arrangement of DNA within the viral capsid.

  15. Flow cytometric measurement of total DNA and incorporated halodeoxyuridine

    DOE Patents [OSTI]

    Dolbeare, Frank A. (Livermore, CA); Gray, Joe W. (Livermore, CA)

    1988-01-01

    A method for the simultaneous flow cytometric measurement of the total DNA content and the level of DNA synthesis in normal and malignant cells is disclosed. The sensitivity of the method allows a study of cell cycle traverse rates for large scale cell populations as well as single cell measurements. A DNA stain such as propidium iodide or Hoechst 33258 is used as the probe for the measurement of total DNA content and a monoclonal antibody reactive with a DNA precursor such as halodeoxy-uridine (HdU), more specifically bromodeoxyuridine (BrdU) is used as a probe for the measurement of HdU or BrdU uptake by the cells as a measure of DNA synthesis.

  16. Advanced uranium enrichment technologies

    SciTech Connect (OSTI)

    Merriman, R.

    1983-03-10

    The Advanced Gas Centrifuge and Atomic Vapor Laser Isotope Separation methods are described. The status and potential of the technologies are summarized, the programs outlined, and the economic incentives are noted. How the advanced technologies, once demonstrated, might be deployed so that SWV costs in the 1990s can be significantly reduced is described.

  17. Gasification: A Cornerstone Technology

    SciTech Connect (OSTI)

    Gary Stiegel

    2008-03-26

    NETL is a leader in the science and technology of gasification - a process for the conversion of carbon-based materials such as coal into synthesis gas (syngas) that can be used to produce clean electrical energy, transportation fuels, and chemicals efficiently and cost-effectively using domestic fuel resources. Gasification is a cornerstone technology of 21st century zero emissions powerplants

  18. Gasification: A Cornerstone Technology

    ScienceCinema (OSTI)

    Gary Stiegel

    2010-01-08

    NETL is a leader in the science and technology of gasification - a process for the conversion of carbon-based materials such as coal into synthesis gas (syngas) that can be used to produce clean electrical energy, transportation fuels, and chemicals efficiently and cost-effectively using domestic fuel resources. Gasification is a cornerstone technology of 21st century zero emissions powerplants

  19. Technologies Team Leader

    E-Print Network [OSTI]

    Wood, Stephen L.

    :(321) 674-8096 Fax: (321) 674-7212 Water Wave Technologies purpose is to develop new renewable energyWater Wave Technologies Team Leader: ­ Khoury Mains (kmains@fit.edu) Members: ­ Sean Cox ­ Michael methods. We chose ocean waves and designed a wing to capture the energy from the wave. The captured energy

  20. Salinity Management Desalination Technology

    E-Print Network [OSTI]

    Scott, Christopher

    Salinity Management and Desalination Technology for Brackish Water Resources in the Arid West.S. Bureau of Reclamation August, 2008 #12;Salinity Management and Desalination Technology for Brackish Water a practical roadmap forward for achieving sustainable, viable desalination of inland, moderate salinity waters

  1. PRESSURE ACTIVATED SEALANT TECHNOLOGY

    SciTech Connect (OSTI)

    Michael A. Romano

    2004-04-01

    The objective of this project is to develop new, efficient, cost effective methods of internally sealing natural gas pipeline leaks through the application of differential pressure activated sealants. In researching the current state of the art for gas pipeline sealing technologies we concluded that if the project was successful, it appeared that pressure activated sealant technology would provide a cost effective alternative to existing pipeline repair technology. From our analysis of current field data for a 13 year period from 1985 to 1997 we were able to identify 205 leaks that were candidates for pressure activated sealant technology, affirming that pressure activated sealant technology is a viable option to traditional external leak repairs. The data collected included types of defects, areas of defects, pipe sizes and materials, incident and operating pressures, ability of pipeline to be pigged and corrosion states. This data, and subsequent analysis, was utilized as a basis for constructing applicable sealant test modeling.

  2. Transposon-containing DNA cloning vector and uses thereof

    DOE Patents [OSTI]

    Berg, Claire M. (W. Willington, CT); Berg, Douglas E. (St. Louis, MO); Wang, Gan (Storrs, CT)

    1997-01-01

    The present invention discloses a rapid method of restriction mapping, sequencing or localizing genetic features in a segment of deoxyribonucleic acid (DNA) that is up to 42 kb in size. The method in part comprises cloning of the DNA segment in a specialized cloning vector and then isolating nested deletions in either direction in vivo by intramolecular transposition into the cloned DNA. A plasmid has been prepared and disclosed.

  3. Transposon-containing DNA cloning vector and uses thereof

    DOE Patents [OSTI]

    Berg, C.M.; Berg, D.E.; Wang, G.

    1997-07-08

    The present invention discloses a rapid method of restriction mapping, sequencing or localizing genetic features in a segment of deoxyribonucleic acid (DNA) that is up to 42 kb in size. The method in part comprises cloning of the DNA segment in a specialized cloning vector and then isolating nested deletions in either direction in vivo by intramolecular transposition into the cloned DNA. A plasmid has been prepared and disclosed. 4 figs.

  4. DNA Methylation as a Biomarker for Preeclampsia

    SciTech Connect (OSTI)

    Anderson, Cindy M.; Ralph, Jody L.; Wright, Michelle L.; Linggi, Bryan E.; Ohm, Joyce E.

    2014-10-01

    Background: Preeclampsia contributes significantly to pregnancy-associated morbidity and mortality as well as future risk of cardiovascular disease in mother and offspring, and preeclampsia in offspring. The lack of reliable methods for early detection limits the opportunities for prevention, diagnosis, and timely treatment. Purpose: The purpose of this study was to explore distinct DNA methylation patterns associated with preeclampsia in both maternal cells and fetal-derived tissue that represent potential biomarkers to predict future preeclampsia and inheritance in children. Method: A convenience sample of nulliparous women (N = 55) in the first trimester of pregnancy was recruited for this prospective study. Genome-wide DNA methylation was quantified in first-trimester maternal peripheral white blood cells and placental chorionic tissue from normotensive women and those with preeclampsia (n = 6/group). Results: Late-onset preeclampsia developed in 12.7% of women. Significant differences in DNA methylation were identified in 207 individual linked cytosine and guanine (CpG) sites in maternal white blood cells collected in the first trimester (132 sites with gain and 75 sites with loss of methylation), which were common to approximately 75% of the differentially methylated CpG sites identified in chorionic tissue of fetal origin. Conclusion: This study is the first to identify maternal epigenetic targets and common targets in fetal-derived tissue that represent putative biomarkers for early detection and heritable risk of preeclampsia. Findings may pave the way for diagnosis of preeclampsia prior to its clinical presentation and acute damaging effects, and the potential for prevention of the detrimental long-term sequelae.

  5. Allosteric Modulation of DNA by Small Molecules

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 OutreachProductswsicloudwsiclouddenDVA N C E D B L O O D S TA I N P A T TSorbentsAllosteric Modulation of DNA by

  6. DNA Origami: A History and Current Perspective

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home Room NewsInformation Current HAB Packet Hanford Advisory Board6/23/2014DLFM libraryDNA Origami

  7. A DNA tweezer-actuated enzyme nanoreactor

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of NaturalDukeWakefieldSulfateSciTechtail.TheoryTuesday, August 10, 20102016 News Below are4BElectron---neutrinoAA CleanAAA DNA

  8. Intriguing DNA Editor Has a Structural Trigger

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration would likeUniverseIMPACT EVALUATION PLANIsProcessRegulationRadiativeIntriguing DNA Editor Has a

  9. Intriguing DNA Editor Has a Structural Trigger

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration would likeUniverseIMPACT EVALUATION PLANIsProcessRegulationRadiativeIntriguing DNA Editor Has

  10. Ergonomics in DNA Sequencing: Standing Down to Ergonomics

    E-Print Network [OSTI]

    Naca, Christine

    2009-01-01

    Ergonomics in DNA SequencingStanding Down to Ergonomics Presented at the COEH 2009We Do: Introduction • How We Work: Ergonomics Challenges in

  11. High-Performance Integrated Genetic Analyzers for Forensic DNA Typing

    E-Print Network [OSTI]

    Liu, Peng

    2009-01-01

    in nanoliter volumes. Analytical Chemistry 72, 5507-5512 (genetic analysis. Analytical Chemistry 78, 5474-5479 (2006).DNA sequencing. Analytical Chemistry 78, 3632-3637 (2006).

  12. Effect of salt concentration on the stability of heterogeneous DNA

    E-Print Network [OSTI]

    Amar Singh; Navin Singh

    2015-09-28

    We study the role of cations on the stability of double stranded DNA (dsDNA) molecules.It is known that the two strands of double stranded DNA(dsDNA) have negative charge due to phosphate group. Cations in the form of salt in the solution, act as shielding agents thereby reducing the repulsion between these strands. We study several heterogeneous DNA molecules. We calculate the phase diagrams for DNA molecules in thermal as well as in force ensembles using Peyrard-Bishop-Dauxois (PBD) model. The dissociation and the stacking energies are the two most important factors that play an important role in the DNA stability. With suitable modifications in the model parameters we investigate the role of cation concentration on the stability of different heterogeneous DNA molecules. The objective of this work is to understand how these cations modify the strength of different pairs or bases along the strand. The phase diagram for the force ensemble case (a dsDNA is pulled from an end) is compared with the experimental results.

  13. Ionic switch controls the DNA state in phage ?

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Li, Dong; Liu, Ting; Zuo, Xiaobing; Li, Tao; Qiu, Xiangyun; Evilevitch, Alex

    2015-06-19

    We have recently found that DNA packaged in phage ? undergoes a disordering transition triggered by temperature, which results in increased genome mobility. This solid-to-fluid like DNA transition markedly increases the number of infectious ? particles facilitating infection. However, the structural transition strongly depends on temperature and ionic conditions in the surrounding medium. Using titration microcalorimetry combined with solution X-ray scattering, we mapped both energetic and structural changes associated with transition of the encapsidated ?-DNA. Packaged DNA needs to reach a critical stress level in order for transition to occur. We varied the stress on DNA in the capsid bymore »changing the temperature, packaged DNA length and ionic conditions. We found striking evidence that the intracapsid DNA transition is ‘switched on’ at the ionic conditions mimicking those in vivo and also at the physiologic temperature of infection at 37°C. This ion regulated on-off switch of packaged DNA mobility in turn affects viral replication. The results suggest a remarkable adaptation of phage ? to the environment of its host bacteria in the human gut. The metastable DNA state in the capsid provides a new paradigm for the physical evolution of viruses.« less

  14. Jefferson Lab Hosts Upcoming Science Lectures on DNA and Chocolate...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    lecture on March 29 titled DNA: The Strand That Connects Us All presented by Matt Kaplan from the Human Origins Genotyping Laboratory, Phoenix, Ariz. Kaplan will discuss how...

  15. Nanoscale topographical replication of graphene architecture by artificial DNA nanostructures

    SciTech Connect (OSTI)

    Moon, Y.; Seo, S.; Park, J.; Park, T.; Ahn, J. R., E-mail: jrahn@skku.edu [Department of Physics, Sungkyunkwan University, Suwon 440-746 (Korea, Republic of); Shin, J.; Dugasani, S. R. [Sungkyunkwan Advanced Institute of Nanotechnology (SAINT), Sungkyunkwan University, Suwon 440-746 (Korea, Republic of); Woo, S. H. [College of Pharmacy, Chungnam National University, Daejeon 305-764 (Korea, Republic of); Park, S. H., E-mail: sunghapark@skku.edu [Department of Physics, Sungkyunkwan University, Suwon 440-746 (Korea, Republic of); Sungkyunkwan Advanced Institute of Nanotechnology (SAINT), Sungkyunkwan University, Suwon 440-746 (Korea, Republic of)

    2014-06-09

    Despite many studies on how geometry can be used to control the electronic properties of graphene, certain limitations to fabrication of designed graphene nanostructures exist. Here, we demonstrate controlled topographical replication of graphene by artificial deoxyribonucleic acid (DNA) nanostructures. Owing to the high degree of geometrical freedom of DNA nanostructures, we controlled the nanoscale topography of graphene. The topography of graphene replicated from DNA nanostructures showed enhanced thermal stability and revealed an interesting negative temperature coefficient of sheet resistivity when underlying DNA nanostructures were denatured at high temperatures.

  16. Fleet DNA Project - Data Dictionary for Public Download Files

    SciTech Connect (OSTI)

    Duran, A.; Burton, E.; Kelly, K.; Walkowicz, K.

    2014-09-01

    Reference document for the Fleet DNA results data shared on the NREL public website. The document includes variable definitions and descriptions to assist users in understanding data.

  17. Selective chemical labelling of natural T modifications in DNA

    E-Print Network [OSTI]

    Hardisty, Robyn E.; Kawasaki, Fumiko; Sahakyan, Aleksandr B.; Balasubramanian, Shankar

    2015-05-06

    enriched, we carried out experiments exploiting the selective reactions developed for probes 1, 2, and 3. A double-stranded 80-mer bearing two modifications per strand was used as a model for 5-fU (fU-DNA), while an analogous ODN containing 5-fC (f... C-DNA) and a non-modified ODN (GCAT-DNA) were used as controls (Supporting Information, Table S1). These ODNs were subjected to the biotinylation reaction followed by affinity enrichment using streptavidin- coated magnetic beads. fU-DNA was enriched over f...

  18. Validation of DNA probes for molecular cytogenetics by mapping onto immobilized circular DNA

    SciTech Connect (OSTI)

    Greulich-Bode, Karin M.; Wang, Mei; Rhein, Andreas P.; Weier, Jingly F.; Weier, Heinz-Ulli G.

    2008-12-04

    Fluorescence in situ hybridization (FISH) is a sensitive and rapid procedure to detect gene rearrangements in tumor cells using non-isotopically labeled DNA probes. Large insert recombinant DNA clones such as bacterial artificial chromosome (BAC) or P1/PAC clones have established themselves in recent years as preferred starting material for probe preparations due to their low rates of chimerism and ease of use. However, when developing probes for the quantitative analysis of rearrangements involving genomic intervals of less than 100kb, careful probe selection and characterization are of paramount importance. We describe a sensitive approach to quality control probe clones suspected of carrying deletions or for measuring clone overlap with near kilobase resolution. The method takes advantage of the fact that P1/PAC/BAC's can be isolated as circular DNA molecules, stretched out on glass slides and fine-mapped by multicolor hybridization with smaller probe molecules. Two examples demonstrate the application of this technique: mapping of a gene-specific {approx}6kb plasmid onto an unusually small, {approx}55kb circular P1 molecule and the determination of the extent of overlap between P1 molecules homologous to the human NF-{kappa}B2 locus. The relatively simple method presented here does not require specialized equipment and may thus find widespread applications in DNA probe preparation and characterization, the assembly of physical maps for model organisms or in studies on gene rearrangements.

  19. Validation of DNA probes for molecular cytogenetics by mapping onto immobilized circular DNA

    SciTech Connect (OSTI)

    Greulich-Bode, Karin; Wang, Mei; Rhein, Andreas; Weier, Jingly; Weier, Heinz-Ulli

    2008-12-16

    Fluorescence in situ hybridization (FISH) is a sensitive and rapid procedure to detect gene rearrangements in tumor cells using non-isotopically labeled DNA probes. Large insert recombinant DNA clones such as bacterial artificial chromosome (BAC) or P1/PAC clones have established themselves in recent years as preferred starting material for probe preparations due to their low rates of chimerism and ease of use. However, when developing probes for the quantitative analysis of rearrangements involving genomic intervals of less than 100kb, careful probe selection and characterization are of paramount importance. We describe a sensitive approach to quality control probe clones suspected of carrying deletions or for measuring clone overlap with near kilobase resolution. The method takes advantage of the fact that P1/PAC/BAC's can be isolated as circular DNA molecules, stretched out on glass slides and fine-mapped by multicolor hybridization with smaller probe molecules. Two examples demonstrate the application of this technique: mapping of a gene-specific {approx}6kb plasmid onto an unusually small, {approx}55kb circular P1 molecule and the determination of the extent of overlap between P1 molecules homologous to the human NF-?B2 locus. The relatively simple method presented here does not require specialized equipment and may thus find widespread applications in DNA probe preparation and characterization, the assembly of physical maps for model organisms or in studies on gene rearrangements.

  20. Technology Innovation Program Advisory Board

    E-Print Network [OSTI]

    Technology Innovation Program Advisory Board 2011 Annual Report of the #12;#12;i 2011 Annual Report of the Technology Innovation Program Advisory Board U.S. Department of Commerce National Institute of Standards and Technology Technology Innovation Program March 2012 #12;ii For Information regarding the Technology

  1. The IDA Technology Stan Franklin

    E-Print Network [OSTI]

    Memphis, University of

    The IDA Technology Stan Franklin and the `Conscious' Software Research Group #12;FedEx Institute of Technology--The IDA Technology 2 Introducing IDA An intelligent software agent capable of entirely of Technology--The IDA Technology 3 IDA Negotiates IDA negotiates with clients in natural language

  2. APPLIED TECHNOLOGY Strategic Plan Summary

    E-Print Network [OSTI]

    Heller, Barbara

    SCHOOL OF APPLIED TECHNOLOGY Strategic Plan Summary #12;School of Applied Technology Strategic Plan Summary | 1 SCHOOL OF APPLIED TECHNOLOGY STRATEGIC PLAN SUMMARY MISSION STATEMENT The mission Technology and Management program to achieve national visibility. #12;School of Applied Technology Strategic

  3. Vehicle Technologies Office: Graduate Automotive Technology Education

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE: Alternative FuelsofProgram: Report1538-1950 TimelineUtility-ScaleTechnology|

  4. Technology development productivity : case studies in technology transition

    E-Print Network [OSTI]

    Taplett, Amanda Kingston

    2007-01-01

    Development of new technology is critical to the growth and success of technology-driven companies. New technology is generated in a number of ways, one of the most important being the company's own internal research and ...

  5. Indian Institute of Technology Bombay INDIAN INSTITUTE OF TECHNOLOGY BOMBAY

    E-Print Network [OSTI]

    Narayanan, H.

    Indian Institute of Technology Bombay INDIAN INSTITUTE OF TECHNOLOGY BOMBAY INVITATION Description of work Estimated cost (1) (2) (3) 1 Construction of Institutional/Residential buildings, external development, HVAC, Elevators etc. for Indian Institute of Technology Bombay, at the campus

  6. Vehicle Technologies Office: 2013 Fuel and Lubricant Technologies...

    Office of Environmental Management (EM)

    3 Fuel and Lubricant Technologies R&D Annual Progress Report Vehicle Technologies Office: 2013 Fuel and Lubricant Technologies R&D Annual Progress Report This report describes the...

  7. Physical properties of naked DNA influence nucleosome positioning and correlate with transcription start and termination sites in yeast

    E-Print Network [OSTI]

    Deniz, Özgen; Flores, Oscar; Battistini, Federica; Pérez, Alberto; Soler-López, Montserrat; Orozco, Modesto

    2011-01-01

    al. : Physical properties of naked DNA influence nucleosomeAccess Physical properties of naked DNA influence nucleosomeelusive. Results: Naked (histone-free) and nucleosomal DNA

  8. Robotics Technology Development Program. Technology summary

    SciTech Connect (OSTI)

    Not Available

    1994-02-01

    The Robotics Technology Development Program (RTDP) is a ``needs-driven`` effort. A lengthy series of presentations and discussions at DOE sites considered critical to DOE`s Environmental Restoration and Waste Management (EM) Programs resulted in a clear understanding of needed robotics applications toward resolving definitive problems at the sites. A detailed analysis of the Tank Waste Retrieval (TWR), Contaminant Analysis Automation (CAA), Mixed Waste Operations (MWO), and Decontamination & Dismantlement (D&D). The RTDP Group realized that much of the technology development was common (Cross Cutting-CC) to each of these robotics application areas, for example, computer control and sensor interface protocols. Further, the OTD approach to the Research, Development, Demonstration, Testing, and Evaluation (RDDT&E) process urged an additional organizational break-out between short-term (1--3 years) and long-term (3--5 years) efforts (Advanced Technology-AT). The RDTP is thus organized around these application areas -- TWR, CAA, MWO, D&D and CC&AT -- with the first four developing short-term applied robotics. An RTDP Five-Year Plan was developed for organizing the Program to meet the needs in these application areas.

  9. DNA-Based Optomechanical Molecular Motor

    SciTech Connect (OSTI)

    McCullagh, Martin; Franco, Ignacio; Ratner, Mark A.; Schatz, George C.

    2011-03-16

    An azobenzene-capped DNA hairpin coupled to an AFM is presented as an optically triggered single-molecule motor. The photoinduced trans to cis isomerization of azobenzene affects both the overall length of the molecule and the ability of the DNA bases to hybridize. Using a combination of molecular dynamics simulations and free energy calculations the unfolding of both isomers along the O5'-O3' extension coordinate is monitored. The potentials of mean force (PMFs) along this coordinate indicate that there are two major differences induced by photoisomerization. The first is that the interbase hydrogen bond and stacking interactions are stable for a greater range of extensions in the trans system than in the cis system. The second difference is due to a decreased chain length of the cis isomer with respect to the trans isomer. These differences are exploited to extract work in optomechanical cycles. The disruption of the hairpin structure gives a maximum of 3.4 kcal mol-1 of extractable work per cycle with an estimated maximum efficiency of 2.4%. Structure-function insights into the operation of this motor are provided, and the effect of the cantilever stiffness on the extractable work is characterized.

  10. Force steps during viral DNA packaging ?

    E-Print Network [OSTI]

    Prashant K. Purohit; Jane' Kondev; Rob Phillips

    2003-09-22

    Biophysicists and structural biologists increasingly acknowledge the role played by the mechanical properties of macromolecules as a critical element in many biological processes. This change has been brought about, in part, by the advent of single molecule biophysics techniques that have made it possible to exert piconewton forces on key macromolecules and observe their deformations at nanometer length scales, as well as to observe the mechanical action of macromolecules such as molecular motors. This has opened up immense possibilities for a new generation of mechanical investigations that will respond to such measurements in an attempt to develop a coherent theory for the mechanical behavior of macromolecules under conditions where thermal and chemical effects are on an equal footing with deterministic forces. This paper presents an application of the principles of mechanics to the problem of DNA packaging, one of the key events in the life cycle of bacterial viruses with special reference to the nature of the internal forces that are built up during the DNA packaging process.

  11. OHVT technology roadmap [2000

    SciTech Connect (OSTI)

    Bradley, R.A.

    2000-02-01

    The OHVT Technology Roadmap for 2000 presents the multiyear program plan of the U.S. DOE's Office of Heavy Vehicle Technologies (OHVT). It is an update of the 1997 plan, reflecting changes in regulations and ongoing discussions with DOE's heavy vehicle customers. The technical plan covers three classes of trucks: (1) class 7-8 (large, on-highway trucks); (2) class 3-6 (medium duty trucks); (3) class 1-2 (pickups, vans, and sport utility vehicles) as well as enabling and supporting technologies. The Roadmap documents program goals, schedules, and milestones.

  12. SYNTHESIS OF THE FULLY PROTECTED PHOSPHORAMIDITE OF THE BENZENE-DNA ADDUCT, N2- (4-HYDROXYPHENYL)-2'-DEOXYGUANOSINE AND INCORPORATION OF THE LATER INTO DNA OLIGOMERS

    SciTech Connect (OSTI)

    Chenna, Ahmed; Gupta, Ramesh C.; Bonala, Radha R.; Johnson, Francis; Huang, Bo

    2008-06-09

    N2-(4-Hydroxyphenyl)-2'-deoxyguanosine-5'-O-DMT-3'-phosphoramidite has been synthesized and used to incorporate the N2-(4-hydroxyphenyl)-2'-dG (N2-4-HOPh-dG) into DNA, using solid-state synthesis technology. The key step to obtaining the xenonucleoside is a palladium (Xantphos-chelated) catalyzed N2-arylation (Buchwald-Hartwig reaction) of a fully protected 2'-deoxyguanosine derivative by 4-isobutyryloxybromobenzene. The reaction proceeded in good yield and the adduct was converted to the required 5'-O-DMT-3'-O-phosphoramidite by standard methods. The latter was used to synthesize oligodeoxynucleotides in which the N2-4-HOPh-dG adduct was incorporated site-specifically. The oligomers were purified by reverse-phase HPLC. Enzymatic hydrolysis and HPLC analysis confirmed the presence of this adduct in the oligomers.

  13. Water Management Technologies from Europe 

    E-Print Network [OSTI]

    Woinsky, S. G.

    2000-01-01

    EPRl is cooperating with European companies to apply their know-how and technologies in the United States. One such alliance involves Pell Frischmann (a UK engineering firm) and BG Technology (a UK technology firm). These firms have worked together...

  14. Sandia Energy - A Green Technology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    A Green Technology Home Energy Research EFRCs Solid-State Lighting Science EFRC A Green Technology A Green TechnologyTara Camacho-Lopez2015-05-11T21:08:32+00:00 Solid-State...

  15. Office of Information Technology Accomplishments

    E-Print Network [OSTI]

    Yorke, James

    Office of Information Technology Accomplishments Fiscal Year 2007 #12;Office of Information'scentralinformationtechnologyserviceorganization,itismypleasuretosharewithyou theOfficeofInformationTechnology'spublication,"OITAccomplishmentsFiscalYear2007,"whichfeatures's goal is to take a quantum leap forward in the information technology environmentavailabletostudents

  16. Wind Technologies & Evolving Opportunities (Presentation)

    SciTech Connect (OSTI)

    Robichaud, R.

    2014-07-01

    This presentation covers opportunities for wind technology; wind energy market trends; an overview of the National Wind Technology Center near Boulder, Colorado; wind energy price and cost trends; wind turbine technology improvements; and wind resource characterization improvements.

  17. February 2000 Advanced Technology Program

    E-Print Network [OSTI]

    of Standards and Technology (NIST) is a cost-sharing program designed to partner the federal governmentFebruary 2000 Advanced Technology Program Information Infrastructure for Healthcare Focused Program: A Brief History ADADVANCEDANCED TECHNOLOGY PRTECHNOLOGY PROGRAMOGRAM NISTIR 6477 National Institute

  18. Energy Technology Engineering Center

    Office of Energy Efficiency and Renewable Energy (EERE)

    The Energy Technology Engineering Center (ETEC) is located within Area IV of the Santa Susana Field Laboratory. The ETEC occupies 90-acres within the 290 acre site. The Santa Susana Field...

  19. Mapping healthcare information technology

    E-Print Network [OSTI]

    Crawford, William Charles Richards

    2010-01-01

    In this thesis I have developed a map of Healthcare Information Technology applications used in the United States for care delivery, healthcare enterprise management, clinical support, research and patient engagement. No ...

  20. Engineering, Architecture & Technology

    E-Print Network [OSTI]

    Engineering, Architecture & Technology Chemistry Engineering and Engineering Sciences Mathematics ECEN 3714 ECEN 4133 IEM 3503 MAE 3123 MAE 3223 EET 1244 BIOC 3713 Chemistry Engineering and Engineering Sciences Mathematics Physics Other Chemistry Engineering and Engineering Sciences Mathematics Physics Other

  1. Clean Coal Technology (Indiana)

    Broader source: Energy.gov [DOE]

    A public utility may not use clean coal technology at a new or existing electric generating facility without first applying for and obtaining from the Utility Regulatory Commission a certificate...

  2. Science & Technology - 2015

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and finally compressed to a short pulse and highest peak power in large compressor vessels. The new front-end technology is based on a short-pulse optical-parametric...

  3. New technologies for neuromodulation

    E-Print Network [OSTI]

    Moini, Azadeh

    2009-01-01

    Non-invasive neural stimulation techniques are of increasing importance as devices move from the lab to the clinical environment. One such technology-transcranial magnetic stimulation-has already made the transition and ...

  4. Membrane Technology Workshop

    Broader source: Energy.gov [DOE]

    At the Membrane Technology Workshop (held July 24, 2012, in Rosemont, IL), stakeholders from industry and academia explored the status of membrane research and development (R&D). Participants ...

  5. Quadrennial Technology Review 2015

    Broader source: Energy.gov (indexed) [DOE]

    program supported improvements in this technology, such as the use of nano-clay for next-generation HVDC cables. A research emphasis is also needed on superconducting HVDC cables,...

  6. Stimulating Energy Technology Innovation

    E-Print Network [OSTI]

    Moniz, Ernest J.

    The innovation system has interrelated components of invention, translation, adoption, and diffusion. Energy technology innovation has lagged that in other domains, and there is a compelling public interest in picking up ...

  7. SCIENCE CHINA Technological Sciences

    E-Print Network [OSTI]

    Liu, Yijun

    turbines, jet engines, nuclear power plants and space crafts, have placed severe demands on highSCIENCE CHINA Technological Sciences © Science China Press and Springer-Verlag Berlin Heidelberg

  8. ENERGY EFFICIENCY TECHNOLOGY ROADMAP

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    n E n v e l o p e This document is one component of the Energy Efficiency Technology Roadmap (EE Roadmap), published by the Bonneville Power Administration (BPA) on behalf of...

  9. ENERGY EFFICIENCY TECHNOLOGY ROADMAP

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    3 : L i g h t i n g This document is one component of the Energy Efficiency Technology Roadmap (EE Roadmap), published by the Bonneville Power Administration (BPA) on behalf of...

  10. Technology catalogue. Second edition

    SciTech Connect (OSTI)

    1995-04-01

    The Department of Energy`s (DOE`s) Office of Environmental Management (EM) is responsible for remediating DOE contaminated sites and managing the DOE waste inventory in a safe and efficient manner. EM`s Office of Technology Development (OTD) supports applied research and demonstration efforts to develop and transfer innovative, cost-effective technologies to its site clean-up and waste-management programs within EM. The purpose of the Technology Catalogue is to: (a) provide performance data on OTD-developed technologies to scientists and engineers responsible for preparing Remedial Investigation/Feasibility Studies (RI/FSs) and other compliance documents for the DOE`s clean-up and waste-management programs; and (b) identify partnering and commercialization opportunities with industry, other federal and state agencies, and the academic community.

  11. TECHNOLOGY PROGRAM PLAN

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    quality. As a component of that effort, the CCRP-administered by the Office of Clean Coal and implemented by the National Energy Technology Laboratory (NETL)-is engaged in...

  12. Agriculture, technology, and conflict 

    E-Print Network [OSTI]

    Zilverberg, Cody John

    2009-05-15

    Conflict and agriculture have a long, shared history. The purpose of this research is to look at the relationships between agriculture, agricultural technologies, and conflict during current and recent conflicts, large scale and localized...

  13. Technology Performance Exchange

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Q3 Deliverable: Develop Site Workflows and Design Website Layout Q3 GoNo-Go DOE Decision Point: Passed Q1 Milestone: Develop Data Entry Forms Q2 Milestone: Release Technology...

  14. Voting Technology and Innovation

    E-Print Network [OSTI]

    Hall, Thad E.

    The 2008 election was different from the last two presidential elections in that there was a clear winner on Election Day and the winner was a Democrat, Barack Obama. Controversies over voting technology that raged in 2000 ...

  15. MECHANICAL ENGINEERING Calculus for Technology I

    E-Print Network [OSTI]

    -2014 Oklahoma State University College of Engineering, Architecture & Technology CEAT Engineering Technology: cc University College of Engineering, Architecture & Technology CEAT Engineering Technology: cc 3MECHANICAL ENGINEERING TECHNOLOGY Name: Advisor: Sem MATH 2123 Calculus for Technology I Grade

  16. SCHOOL OF ENGINEERING TECHNOLOGY Surveying Engineering

    E-Print Network [OSTI]

    Thomas, Andrew

    SCHOOL OF ENGINEERING TECHNOLOGY Surveying Engineering Technology practice FOCUSED WHY SURVEYING ENGINEERING TECHNOLOGY? Surveying engineering technology is a practice- focused program that provides students ENGINEERING TECHNOLOGY DEGREE? A graduate with a surveying engineering technology degree can work as a party

  17. ORIGINAL ARTICLE Forensic DNA databasesEthical and legal

    E-Print Network [OSTI]

    Noble, William Stafford

    number of innocent people's records to be kept, became highly controversial.1­3 * Corresponding author Authority. #12;Under former UK Prime Minister Tony Blair, legislation was introduced as part of the Criminal's DNA profiles to be retained on what was previously a criminal DNA database, overturning the pre

  18. Molecular Devices A Unidirectional DNA Walker That Moves

    E-Print Network [OSTI]

    Yin, Peng

    a designated path. The successful construction of self-assembled DNA nanostructures provides a solid structural,2] In particular, recent years have seen remarkable success in the construction of both self-assembled that moves along a DNA track. The self-assembled track contains three anchor- ages at which the walker, a six

  19. Alternative Methods for Human Identification: Mitochondrial DNA Base Composition Profiling

    E-Print Network [OSTI]

    Perkins, Richard A.

    of nucleotides ­ Base composition of a DNA molecule can be inferred ­ An empirical formula of numbers of A, G, C) · Fully automated ­ Plate stacker holds up to 15 PCR plates ­ Desalting by magnetic bead cleanup · Cleanup are dissociated on injection · DNA molecular masses are measured ­ Forward and reverse strands measured separately

  20. DNA damage in cells exhibiting radiation-induced genomic instability

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Keszenman, Deborah J.; Kolodiuk, Lucia; Baulch, Janet E.

    2015-02-22

    Cells exhibiting radiation induced genomic instability exhibit varied spectra of genetic and chromosomal aberrations. Even so, oxidative stress remains a common theme in the initiation and/or perpetuation of this phenomenon. Isolated oxidatively modified bases, abasic sites, DNA single strand breaks and clustered DNA damage are induced in normal mammalian cultured cells and tissues due to endogenous reactive oxygen species generated during normal cellular metabolism in an aerobic environment. While sparse DNA damage may be easily repaired, clustered DNA damage may lead to persistent cytotoxic or mutagenic events that can lead to genomic instability. In this study, we tested the hypothesismore »that DNA damage signatures characterised by altered levels of endogenous, potentially mutagenic, types of DNA damage and chromosomal breakage are related to radiation-induced genomic instability and persistent oxidative stress phenotypes observed in the chromosomally unstable progeny of irradiated cells. The measurement of oxypurine, oxypyrimidine and abasic site endogenous DNA damage showed differences in non-double-strand breaks (DSB) clusters among the three of the four unstable clones evaluated as compared to genomically stable clones and the parental cell line. These three unstable clones also had increased levels of DSB clusters. The results of this study demonstrate that each unstable cell line has a unique spectrum of persistent damage and lead us to speculate that alterations in DNA damage signaling and repair may be related to the perpetuation of genomic instability.« less

  1. Redesigning the Specificity of ProteinDNA Interactions with Rosetta

    E-Print Network [OSTI]

    Baker, David

    +Business Media New York 2014 #12;266 DNA cleavage enzymes, such as LAGLIDADG endonucleases [4, 5], to sites specificity offer an efficient alternative to more labor-intensive experimental procedures, such as directed­DNA specificity.Each panel represents the structure with the mean energy from a set of 56 repacks done

  2. DNA topology confers sequence specificity to nonspecific architectural proteins

    E-Print Network [OSTI]

    Swigon, David

    DNA topology confers sequence specificity to nonspecific architectural proteins Juan Weia , Luke proteins into tightly organized 3D struc- tures. The bacterial heat-unstable (HU) protein builds up. The HU protein introduces a unique spatial pathway in the DNA upon closure. The many ways in which

  3. Short communication The evolution of DNA databases--Recommendations

    E-Print Network [OSTI]

    Short communication The evolution of DNA databases--Recommendations for new European STR loci Peter from 7 to 10. # 2005 Elsevier Ireland Ltd. All rights reserved. Keywords: National DNA database; STR; Evolution; European standards 1. Introduction The European Network of Forensic Science Institutes (ENFSI

  4. Shape and energetics of DNA plectonemes Prashant K. Purohit

    E-Print Network [OSTI]

    Purohit, Prashant

    , including single molecule experiments, and it is known that the average bending modulus of a random sequence scales of few hundred nanometers is described by a fluctuating elastic rod model. In this paper we couple that the twisting modulus of DNA is around Kt = 431pNnm2 [12]. The fact that DNA is a twist storing polymer allows

  5. cDNA encoding a polypeptide including a hevein sequence

    DOE Patents [OSTI]

    Raikhel, N.V.; Broekaert, W.F.; Namhai Chua; Kush, A.

    1993-02-16

    A cDNA clone (HEV1) encoding hevein was isolated via polymerase chain reaction (PCR) using mixed oligonucleotides corresponding to two regions of hevein as primers and a Hevea brasiliensis latex cDNA library as a template. HEV1 is 1,018 nucleotides long and includes an open reading frame of 204 amino acids.

  6. DNA barcoding and the renaissance of taxonomy Scott E. Miller*

    E-Print Network [OSTI]

    DNA barcoding and the renaissance of taxonomy Scott E. Miller* Office of the Under Secretary representing field ecology, molecular genetics, and morphological taxonomy presents data in this issue of PNAS). The work of Smith et al. (2) is a display of integrated taxonomy, demon- strating how DNA barcoding

  7. Google matrix analysis of DNA sequences Vivek Kandiah1

    E-Print Network [OSTI]

    Shepelyansky, Dima

    1 Google matrix analysis of DNA sequences Vivek Kandiah1 , Dima L. Shepelyansky1, 1 Laboratoire de.quantware.ups-tlse.fr/dima Abstract For DNA sequences of various species we construct the Google matrix G of Markov tran- sitions between nearby words composed of several letters. The statistical distribution of matrix elements

  8. Semiflexible Polymers: Fundamental Theory and Applications in DNA Packaging

    E-Print Network [OSTI]

    Straight, Aaron

    Semiflexible Polymers: Fundamental Theory and Applications in DNA Packaging Thesis by Andrew James of perfectly flexible and perfectly rigid polymer chains; however, many polymers, for example DNA, are somewhere in between these two limiting cases. Such polymers are termed semiflexible, and their molecular

  9. 2006 Nature Publishing Group Mechanochemical analysis of DNA gyrase using

    E-Print Network [OSTI]

    Gore, Jeff

    -independent step at the end of the reaction cycle, and an ATP-binding step in the middle of the cycle, subsequent of DNA gyrase, which works against mechanical stresses to drive the genome into an elastically strained releasing the DNA substrate), the location of the rate-limiting step for the overall reaction cycle

  10. Hot Technology, Cool Science (LBNL Science at the Theater)

    ScienceCinema (OSTI)

    None available

    2011-04-28

    Great innovations start with bold ideas. Learn how Berkeley Lab scientists are devising practical solutions to everything from global warming to how you get to work. On May 11, 2009, five Berkeley Lab scientists participated in a roundtable dicussion ? moderated by KTVU's John Fowler ? on their leading-edge research. This "Science at the Theater" event, held at the Berkeley Repertory Theatre, featured technologies such as cool roofs, battery-driven transportation, a pocket-sized DNA probe, green supercomputing, and a noncontact method for restoring damaged and fragile mechanical recordings.

  11. Office of Technology Transitions

    Broader source: Energy.gov [DOE]

    DOE's Technology Commercialization activities in 2009-13 have involved three broad areas of focus. The primary focus of technology commercialization has continued to be through new technologies developed at the National Laboratories and Facilities. As a second focus, to support and streamline commercialization of these DOE technologies, DOE has carried out a number of new initiatives and pilot projects. Finally, DOE's Department-wide commitment to using commercialization as one mechanism to support U.S. economic growth has led to new cross-cutting programs. U.S. Department of Energy researchers won 31 of the 100 awards in 2014, 36 awards in each of 2013, 2012 and 2011, and 46 in 2010, for a total of 185 over the period of 2009-13. A subset of these awards and other DOE developed technologies are described in Appendix E. These represent a spectrum of commercial areas including DOE mission areas of energy, efficiency, environment and security, as well as spin-off applications in the agricultural, aeronautical, medical, semiconductor and information technology industries, and broad applications in cyber security and sensing/control systems.

  12. Nuclear Reactors and Technology

    SciTech Connect (OSTI)

    Cason, D.L.; Hicks, S.C. [eds.

    1992-01-01

    This publication Nuclear Reactors and Technology (NRT) announces on a monthly basis the current worldwide information available from the open literature on nuclear reactors and technology, including all aspects of power reactors, components and accessories, fuel elements, control systems, and materials. This publication contains the abstracts of DOE reports, journal articles, conference papers, patents, theses, and monographs added to the Energy Science and Technology Database during the past month. Also included are US information obtained through acquisition programs or interagency agreements and international information obtained through the International Energy Agency`s Energy Technology Data Exchange or government-to-government agreements. The digests in NRT and other citations to information on nuclear reactors back to 1948 are available for online searching and retrieval on the Energy Science and Technology Database and Nuclear Science Abstracts (NSA) database. Current information, added daily to the Energy Science and Technology Database, is available to DOE and its contractors through the DOE Integrated Technical Information System. Customized profiles can be developed to provide current information to meet each user`s needs.

  13. Technology's Impact on Production

    SciTech Connect (OSTI)

    Rachel Amann; Ellis Deweese; Deborah Shipman

    2009-06-30

    As part of a cooperative agreement with the United States Department of Energy (DOE) - entitled Technology's Impact on Production: Developing Environmental Solutions at the State and National Level - the Interstate Oil and Gas Compact Commission (IOGCC) has been tasked with assisting state governments in the effective, efficient, and environmentally sound regulation of the exploration and production of natural gas and crude oil, specifically in relation to orphaned and abandoned wells and wells nearing the end of productive life. Project goals include: (1) Developing (a) a model framework for prioritization and ranking of orphaned or abandoned well sites; (b) a model framework for disbursement of Energy Policy Act of 2005 funding; and (c) a research study regarding the current status of orphaned wells in the nation. (2) Researching the impact of new technologies on environmental protection from a regulatory perspective. Research will identify and document (a) state reactions to changing technology and knowledge; (b) how those reactions support state environmental conservation and public health; and (c) the impact of those reactions on oil and natural gas production. (3) Assessing emergent technology issues associated with wells nearing the end of productive life. Including: (a) location of orphaned and abandoned well sites; (b) well site remediation; (c) plugging materials; (d) plug placement; (e) the current regulatory environment; and (f) the identification of emergent technologies affecting end of life wells. New Energy Technologies - Regulating Change, is the result of research performed for Tasks 2 and 3.

  14. Mapping Technology Space by Normalizing Technology Relatedness Networks

    E-Print Network [OSTI]

    Alstott, Jeff; Yan, Bowen; Luo, Jianxi

    2015-01-01

    Technology is a complex system, with technologies relating to each other in a space that can be mapped as a network. The technology relatedness network's structure can reveal properties of technologies and of human behavior, if it can be mapped accurately. Technology networks have been made from patent data, using several measures of relatedness. These measures, however, are influenced by factors of the patenting system that do not reflect technologies or their relatedness. We created technology networks that precisely controlled for these impinging factors and normalized them out, using data from 3.9 million patents. The normalized technology relatedness networks were sparse, with only ~20% of technology domain pairs more related than would be expected by chance. Different measures of technology relatedness became more correlated with each other after normalization, approaching a single dimension of technology relatedness. The normalized network corresponded with human behavior: we analyzed the patenting his...

  15. Investigating the Role of Vitamin D and DNA Repair in Influencing Cancer Presentation and Outcomes 

    E-Print Network [OSTI]

    Syed, Moinuddin Mohammed

    2014-09-26

    differences in DNA repair genes exist, which may in turn contribute to underlying differences in DNA repair capacity. These genes with high Fst value represent all six known common DNA repair pathways and DDR response. This supports our hypothesis...

  16. The role of the de novo DNA methyltransferase Dnmt3a in the nervous system

    E-Print Network [OSTI]

    Nguyen, Suzanne Pham

    2007-01-01

    DNA methylation is an important mechanism of gene regulation. Evidence is mounting that epigenetic mechanisms including that of DNA methylation operate in the nervous system. Genetic disruption of the de novo DNA ...

  17. Telomere Regulation in Arabidopsis thaliana by the CST Capping Complex and DNA Damage Response Proteins 

    E-Print Network [OSTI]

    Boltz, Kara A.

    2013-09-11

    The ends of chormosomes are capped by telomeres, which distinguish the termini from damaged DNA. Paradoxically, DNA repair proteins are also required for telomere maintenance. How DNA repair pathways are regulated to ...

  18. Morphological taxonomy, DNA barcoding, and species diversity in southern Rocky Mountain headwater streams

    E-Print Network [OSTI]

    Zamudio, Kelly R.

    Morphological taxonomy, DNA barcoding, and species diversity in southern Rocky Mountain headwater and Conditions #12;MOLECULAR APPROACHES IN FRESHWATER ECOLOGY Morphological taxonomy, DNA barcoding, and species: diversity, elevation, DNA barcoding, taxonomy, aquatic insect, EPT, southern Rocky Mountain Elevation

  19. Extending the realm of SuNS to DNA nanoarrays and peptide features

    E-Print Network [OSTI]

    Akbulut Halatci, Özge

    2010-01-01

    Intense research on DNA arrays has been fostered by their applications in the field of biomedicine. DNA microarrays are composed of several different DNA sequences to be analyzed in parallel allowing high throughput ...

  20. Structure of DNA-Bound FEN1 Reveals Mechanism of Action

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Structure of DNA-Bound FEN1 Reveals Mechanism of Action Structure of DNA-Bound FEN1 Reveals Mechanism of Action Print Tuesday, 24 January 2012 11:30 DNA replication is a critical...

  1. Climbing Up the Technology Ladder? High-Technology Exports in China and Latin America

    E-Print Network [OSTI]

    Gallagher, Kevin P.; Porzecanski, Roberto

    2008-01-01

    the Technology Ladder? High- Technology Exports in China andthe Technology Ladder? High-Technology Exports in China andin the global market for high technology products? How does

  2. Science and technology news Nanotechnology

    E-Print Network [OSTI]

    Science and technology news Home Nanotechnology Physics Space & Earth Electronics Technology, nanotechnology and medical diagnostics. Magnetism observed in gas for the first time http

  3. Energy Technologies | Department of Energy

    Office of Environmental Management (EM)

    Technologies Image of industrial pipes. District energy technologies-such as combined heat and power and microgrids-can help state, local, and tribal governments effectively...

  4. Vehicle Technologies Office: Information Resources

    Broader source: Energy.gov [DOE]

    From here you can access additional information on advanced transportation technologies; view programmatic publications and technical information; learn the basics of hybrid vehicle technology;...

  5. Video Resources on Geothermal Technologies

    Broader source: Energy.gov [DOE]

    Geothermal video offerings at the Department of Energy include simple interactive illustrations of geothermal power technologies and interviews on initiatives in the Geothermal Technologies Office.

  6. Technology Licensing Contacts | Partnerships | ORNL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Staff SHARE Technology Licensing Contacts Name TitlePosition Email Address Phone Number Biography Mike Paulus Director, Technology Transfer paulusmj@ornl.gov (865) 574-1051...

  7. Technology Commercialization Showcase - EERE Commercialization...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    the business community. If no one learns of a technology's promise, then that technology will indefinitely sit on the lab shelf. The Solution: The Department Of Energy...

  8. Technology Assistance Program | Partnerships | ORNL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Assistance Program SHARE Technology Assistance Program Electronics Research Assistance is available for small business licensees of ORNL technologies to leverage ORNL's expertise...

  9. Materials Technologies: Goals, Strategies, and Top Accomplishments...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Materials Technologies: Goals, Strategies, and Top Accomplishments (Brochure), Vehicle Technologies Program (VTP) Materials Technologies: Goals, Strategies, and Top Accomplishments...

  10. Sandia Energy - Conventional Water Power: Technology Development

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Technology Development Home Stationary Power Energy Conversion Efficiency Water Power Conventional Water Power: Technology Development Conventional Water Power: Technology...

  11. Implementing Advances in Transport Security Technologies | Department...

    Office of Environmental Management (EM)

    Implementing Advances in Transport Security Technologies Implementing Advances in Transport Security Technologies Implementing Advances in Transport Security Technologies More...

  12. Fact Sheet: Energy Storage Technology Advancement Partnership...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Technology Advancement Partnership (October 2012) Fact Sheet: Energy Storage Technology Advancement Partnership (October 2012) The Energy Storage Technology Advancement Partnership...

  13. Quadrennial Technology Review Workshops | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Quadrennial Technology Review Workshops Quadrennial Technology Review Workshops Department of Energy Quadrennial Technology Review Building & Industrial Efficiency Workshop...

  14. Microearthquake Technology for EGS Fracture Characterization...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Microearthquake Technology for EGS Fracture Characterization; 2010 Geothermal Technology Program Peer Review Report Microearthquake Technology for EGS Fracture Characterization;...

  15. Transaction Costs, Information Technology and Development

    E-Print Network [OSTI]

    Singh, Nirvikar

    2004-01-01

    Transaction Costs, Information Technology and Development 1.Transaction Costs, Information Technology and DevelopmentTransaction Costs, Information Technology and Development *

  16. Transaction Costs, Information Technology and Development

    E-Print Network [OSTI]

    Singh, Nirvikar

    2004-01-01

    Transaction Costs, Information Technology and Development 1.Transaction Costs, Information Technology and DevelopmentTransaction Costs, Information Technology and Development

  17. Advanced Technology Vehicles Manufacturing (ATVM) Loan Program...

    Office of Environmental Management (EM)

    Advanced Technology Vehicles Manufacturing (ATVM) Loan Program Advanced Technology Vehicles Manufacturing (ATVM) Loan Program Advanced Technology Vehicles Manufacturing (ATVM) Loan...

  18. NREL: Technology Transfer - Technologies Available for Licensing

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration wouldMass map shines lightGeospatial ToolkitSMARTS -BeingFuture for SolarTechnologyNew Amber

  19. NREL: Technology Transfer - Technologies Available for Licensing

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home Room NewsInformationJessework usesof EnergyY-12WorkingSolarTechnologies Available for Licensing

  20. NREL: Technology Transfer - Technology Partnership Agreements

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home Room NewsInformationJessework usesof EnergyY-12WorkingSolarTechnologies Available for

  1. Sandia National Laboratories: Training and Technology Demonstration...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Training and Technology Demonstration Area Training and Technology Demonstration Area Sandia's Training and Technology Demonstration Area (TTD) showcases technologies that can be...

  2. 2012 DOE Vehicle Technologies Office Annual Merit Review | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    and Electrical Machines Technologies 4. Advanced Combustion Engine Technologies 5. Fuels and Lubricants Technologies 6. Materials Technologies 7. Materials Technologies:...

  3. 2009 DOE Vehicle Technologies Office Annual Merit Review | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    & Electrical Machines Technologies 4. Advanced Combustion Engine Technologies 5. Fuels and Lubricants Technologies 6. Materials Technologies 7. Materials Technologies:...

  4. 2010 DOE Vehicle Technologies Office Annual Merit Review | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    & Electrical Machines Technologies 4. Advanced Combustion Engine Technologies 5. Fuels and Lubricants Technologies 6. Materials Technologies 7. Materials Technologies:...

  5. 2011 DOE Vehicle Technologies Office Annual Merit Review | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    and Electrical Machines Technologies 4. Advanced Combustion Engine Technologies 5. Fuels and Lubricants Technologies 6. Materials Technologies 7. Materials Technologies:...

  6. Hybridization and Selective Release of DNA Microarrays

    SciTech Connect (OSTI)

    Beer, N R; Baker, B; Piggott, T; Maberry, S; Hara, C M; DeOtte, J; Benett, W; Mukerjee, E; Dzenitis, J; Wheeler, E K

    2011-11-29

    DNA microarrays contain sequence specific probes arrayed in distinct spots numbering from 10,000 to over 1,000,000, depending on the platform. This tremendous degree of multiplexing gives microarrays great potential for environmental background sampling, broad-spectrum clinical monitoring, and continuous biological threat detection. In practice, their use in these applications is not common due to limited information content, long processing times, and high cost. The work focused on characterizing the phenomena of microarray hybridization and selective release that will allow these limitations to be addressed. This will revolutionize the ways that microarrays can be used for LLNL's Global Security missions. The goals of this project were two-fold: automated faster hybridizations and selective release of hybridized features. The first study area involves hybridization kinetics and mass-transfer effects. the standard hybridization protocol uses an overnight incubation to achieve the best possible signal for any sample type, as well as for convenience in manual processing. There is potential to significantly shorten this time based on better understanding and control of the rate-limiting processes and knowledge of the progress of the hybridization. In the hybridization work, a custom microarray flow cell was used to manipulate the chemical and thermal environment of the array and autonomously image the changes over time during hybridization. The second study area is selective release. Microarrays easily generate hybridization patterns and signatures, but there is still an unmet need for methodologies enabling rapid and selective analysis of these patterns and signatures. Detailed analysis of individual spots by subsequent sequencing could potentially yield significant information for rapidly mutating and emerging (or deliberately engineered) pathogens. In the selective release work, optical energy deposition with coherent light quickly provides the thermal energy to single spots to release hybridized DNA. This work leverages LLNL expertise in optics, microfluids, and bioinformatics.

  7. Ceramic Technology Project

    SciTech Connect (OSTI)

    Not Available

    1992-03-01

    The Ceramic Technology Project was developed by the USDOE Office of Transportation Systems (OTS) in Conservation and Renewable Energy. This project, part of the OTS's Materials Development Program, was developed to meet the ceramic technology requirements of the OTS's automotive technology programs. Significant accomplishments in fabricating ceramic components for the USDOE and NASA advanced heat engine programs have provided evidence that the operation of ceramic parts in high-temperature engine environments is feasible. These programs have also demonstrated that additional research is needed in materials and processing development, design methodology, and data base and life prediction before industry will have a sufficient technology base from which to produce reliable cost-effective ceramic engine components commercially. A five-year project plan was developed with extensive input from private industry. In July 1990 the original plan was updated through the estimated completion of development in 1993. The objective is to develop the industrial technology base required for reliable ceramics for application in advanced automotive heat engines. The project approach includes determining the mechanisms controlling reliability, improving processes for fabricating existing ceramics, developing new materials with increased reliability, and testing these materials in simulated engine environments to confirm reliability. Although this is a generic materials project, the focus is on the structural ceramics for advanced gas turbine and diesel engines, ceramic bearings and attachments, and ceramic coatings for thermal barrier and wear applications in these engines. To facilitate the rapid transfer of this technology to US industry, the major portion of the work is being done in the ceramic industry, with technological support from government laboratories, other industrial laboratories, and universities.

  8. A novel role for the transcriptional modulator NusA in DNA repair/damage tolerance pathways in Escherichia coli

    E-Print Network [OSTI]

    Cohen, Susan E., Ph. D. Massachusetts Institute of Technology

    2009-01-01

    All organisms must contend with the consequences of DNA damage, induced by a variety of both endogenous and exogenous sources. Mechanisms of DNA repair and DNA damage tolerance are crucial for cellular survival after DNA ...

  9. Trusted Computing Technologies, Intel Trusted Execution Technology.

    SciTech Connect (OSTI)

    Guise, Max Joseph; Wendt, Jeremy Daniel

    2011-01-01

    We describe the current state-of-the-art in Trusted Computing Technologies - focusing mainly on Intel's Trusted Execution Technology (TXT). This document is based on existing documentation and tests of two existing TXT-based systems: Intel's Trusted Boot and Invisible Things Lab's Qubes OS. We describe what features are lacking in current implementations, describe what a mature system could provide, and present a list of developments to watch. Critical systems perform operation-critical computations on high importance data. In such systems, the inputs, computation steps, and outputs may be highly sensitive. Sensitive components must be protected from both unauthorized release, and unauthorized alteration: Unauthorized users should not access the sensitive input and sensitive output data, nor be able to alter them; the computation contains intermediate data with the same requirements, and executes algorithms that the unauthorized should not be able to know or alter. Due to various system requirements, such critical systems are frequently built from commercial hardware, employ commercial software, and require network access. These hardware, software, and network system components increase the risk that sensitive input data, computation, and output data may be compromised.

  10. Statistical Mutation Calling from Sequenced Overlapping DNA Pools in TILLING Experiments

    E-Print Network [OSTI]

    Missirian, Victor; Comai, Luca; Filkov, Vladimir

    2011-01-01

    S, Pe’er I: Overlapping pools for high-throughput targetedSequenced Overlapping DNA Pools in TILLING Experiments. BMCgeneration resequencing of DNA pools. Bioinformatics 2010,

  11. Sensitive method for measurement of telomeric DNA content in human tissues

    DOE Patents [OSTI]

    Bryant, Jennifer E. (Albuquerque, NM); Hutchings, Kent G. (Albuquerque, NM); Moyzis, Robert K. (Corona Del Mar, CA); Griffith, Jeffrey K. (Cedar Crest, NM)

    1999-02-16

    A sensitive method for measurement of telomeric DNA content in human tissue, based upon the ratio of telomeric to centromeric DNA present in the tissue.

  12. FIELD-BASED TECHNOLOGY EDUCATION: JUST IN TIME TECHNOLOGY TRAINING

    E-Print Network [OSTI]

    Larkin, Teresa L.

    FIELD-BASED TECHNOLOGY EDUCATION: JUST IN TIME TECHNOLOGY TRAINING Sarah Irvine Belson1 and Teresa, Audio Technology, and Physics, American University, 4400 Massachusetts Ave. NW, Washington, DC 20016, tlarkin@american.edu Abstract -- This paper outlines the current status of technology integration

  13. Roadmap: Engineering Technology -Electronics Engineering Technology -Bachelor of Science

    E-Print Network [OSTI]

    Khan, Javed I.

    36620 Project Management in Engineering and Technology 3 ! TECH 33363 Metallurgy and Materials Science

  14. Japan Advanced Institute of Science and Technology Nano Materials Technology

    E-Print Network [OSTI]

    Ogawa, Mizuhito

    started in April 2002 as a renewal of the former Center for New Materials originally established as oneJapan Advanced Institute of Science and Technology Nano Materials Technology (Lecture) Course Center for Nano Materials and Technology #12;The Center for Nano Materials and Technology (CNMT) has

  15. Technology Available for Licensing Office of Technology Management

    E-Print Network [OSTI]

    Maroncelli, Mark

    Technology Available for Licensing Office of Technology Management The Pennsylvania State University 113 Technology Center, University Park, PA 16802 814.865.6277 phone; 814.865.3591 fax Contact: Matthew D. Smith Sr. Technology Licensing Officer The Pennsylvania State University Phone: (814) 863

  16. NASA Earth Science Technology Office (ESTO) Decadal Survey Technology Investments

    E-Print Network [OSTI]

    Christian, Eric

    investments · Risks are retired before major dollars are invested: a cost-effective approach to technologyNASA Earth Science Technology Office (ESTO) Decadal Survey Technology Investments January 7, 2009 #12;Overview: Earth Science Technology Office Science Driven, Competed, Actively Managed

  17. Fleet DNA Project Data Summary Report (Presentation)

    SciTech Connect (OSTI)

    Walkowicz, K.; Duran, A.; Burton, E.

    2014-04-01

    This presentation includes graphical data summaries that highlight statistical trends for medium- and heavy-duty commercial fleet vehicles operating in a variety of vocations. It offers insight for the development of vehicle technologies that reduce costs, fuel consumption, and emission.

  18. Chief Technology Officer Opportunity Profile

    E-Print Network [OSTI]

    Mazzotti, Frank

    Chief Technology Officer Opportunity Profile #12;CHIEF TECHNOLOGY OFFICER Date: 01/23/2015 Prepared for the position and to give a deeper understanding of the role of Chief Technology Officer. We have also included: POSITION ANNOUNCEMENT TAB V: THE REGION TAB I: POSITION ANNOUNCEMENT #12;Chief Technology Officer

  19. Digital Technology and Culture Program

    E-Print Network [OSTI]

    Collins, Gary S.

    Digital Technology and Culture Program College of Arts and Sciences Degree Options Bachelor of Arts in Digital Technology and Culture Minors Digital Technology and Culture Program Strengths · Demonstrate competency with technology for designing and distributing digital works in various mediums. · Demonstrate

  20. SCANNING THE TECHNOLOGY Scanning Advanced

    E-Print Network [OSTI]

    , electronics and software technologies as shown in Fig. 2. A coarse history of the automobile reveals the broadSCANNING THE TECHNOLOGY Scanning Advanced Automobile Technology BY HAMID GHARAVI National Institute of Standards and Technology Guest Editor K. VENKATESH PRASAD Ford Motor Company Guest Editor PETROS IOANNOU

  1. Automation Direct Technology Scholarship Fund

    E-Print Network [OSTI]

    Kostic, Milivoje M.

    in Manufacturing Engineering Technology or Industrial Management & Technology (CAD or Manufacturing area of study or Industrial Management & Technology (CAD or Manufacturing area of study). Candidates must have a minimum NIU's university account. Eligibility: Candidates must be a declared major in Manufacturing Engineering Technology

  2. 2008 Solar Technologies Market Report

    E-Print Network [OSTI]

    Price, S.

    2010-01-01

    Photovoltaics subprogram 84 invests in technologies across the development pipeline that demonstrate progress

  3. Design with Uncertain Technology Evolution 

    E-Print Network [OSTI]

    Arendt, Jonathan Lee

    2012-10-19

    of technology. Techniques for modeling evolution of a technology that has multiple performance attributes are developed. An S-curve technology evolution model is used. The performance of a technology develops slowly at first, quickly during heavy R&D effort...

  4. TECHNOLOGY TRANSFER: PROBLEMS AND PROSPECTS

    E-Print Network [OSTI]

    TECHNOLOGY TRANSFER: PROBLEMS AND PROSPECTS Jesse w. Fussell Department of Defense 9800 Savage Road of technology transfer in this technical area in the past, to forecast prospects for technology transfer in the future, and to suggest some ideas for stimulating the process. 2. TECHNOLOGY TRANSFER PROBLEMS Many

  5. Technology Deployment Annual Report 2010

    SciTech Connect (OSTI)

    Keith Arterburn

    2010-12-01

    This report is a catalog of selected INL technology transfer and commercialization transactions during FY-2010.

  6. DNA repair: Dynamic defenders against cancer and aging

    SciTech Connect (OSTI)

    Fuss, Jill O.; Cooper, Priscilla K.

    2006-04-01

    You probably weren't thinking about your body's cellular DNA repair systems the last time you sat on the beach in the bright sunshine. Fortunately, however, while you were subjecting your DNA to the harmful effects of ultraviolet light, your cells were busy repairing the damage. The idea that our genetic material could be damaged by the sun was not appreciated in the early days of molecular biology. When Watson and Crick discovered the structure of DNA in 1953 [1], it was assumed that DNA is fundamentally stable since it carries the blueprint of life. However, over 50 years of research have revealed that our DNA is under constant assault by sunlight, oxygen, radiation, various chemicals, and even our own cellular processes. Cleverly, evolution has provided our cells with a diverse set of tools to repair the damage that Mother Nature causes. DNA repair processes restore the normal nucleotide sequence and DNA structure of the genome after damage [2]. These responses are highly varied and exquisitely regulated. DNA repair mechanisms are traditionally characterized by the type of damage repaired. A large variety of chemical modifications can alter normal DNA bases and either lead to mutations or block transcription if not repaired, and three distinct pathways exist to remove base damage. Base excision repair (BER) corrects DNA base alterations that do not distort the overall structure of the DNA helix such as bases damaged by oxidation resulting from normal cellular metabolism. While BER removes single damaged bases, nucleotide excision repair (NER) removes short segments of nucleotides (called oligonucleotides) containing damaged bases. NER responds to any alteration that distorts the DNA helix and is the mechanism responsible for repairing bulky base damage caused by carcinogenic chemicals such as benzo [a]pyrene (found in cigarette smoke and automobile exhaust) as well as covalent linkages between adjacent pyrimidine bases resulting from the ultraviolet (UV) component of sunlight. NER can be divided into two classes based on where the repair occurs. NER occurring in DNA that is not undergoing transcription (i.e., most of the genome) is called global genome repair (GGR or GGNER), while NER taking place in the transcribed strand of active genes is called transcription-coupled repair (TCR or TC-NER). We will explore NER in more detail below. Mismatch repair (MMR) is another type of excision repair that specifically removes mispaired bases resulting from replication errors. DNA damage can also result in breaks in the DNA backbone, in one or both strands. Single-strand breaks (SSBs) are efficiently repaired by a mechanism that shares common features with the later steps in BER. Double-strand breaks (DSBs) are especially devastating since by definition there is no intact complementary strand to serve as a template for repair, and even one unrepaired DSB can be lethal [3]. In cells that have replicated their DNA prior to cell division, the missing information can be supplied by the duplicate copy, or sister chromatid, and DSBs in these cells are faithfully repaired by homologous recombination involving the exchange of strands of DNA between the two copies. However, most cells in the body are non-dividing, and in these cells the major mechanism for repairing DSBs is by non-homologous end joining (NHEJ), which as the name implies involves joining two broken DNA ends together without a requirement for homologous sequence and which therefore has a high potential for loss of genetic information.

  7. Glovebox decontamination technology comparison

    SciTech Connect (OSTI)

    Quintana, D.M.; Rodriguez, J.B.; Cournoyer, M.E.

    1999-09-26

    Reconfiguration of the CMR Building and TA-55 Plutonium Facility for mission requirements will require the disposal or recycle of 200--300 gloveboxes or open front hoods. These gloveboxes and open front hoods must be decontaminated to meet discharge limits for Low Level Waste. Gloveboxes and open front hoods at CMR have been painted. One of the deliverables on this project is to identify the best method for stripping the paint from large numbers of gloveboxes. Four methods being considered are the following: conventional paint stripping, dry ice pellets, strippable coatings, and high pressure water technology. The advantages of each technology will be discussed. Last, cost comparisons between the technologies will be presented.

  8. Technology transfer 1995

    SciTech Connect (OSTI)

    Not Available

    1995-01-01

    Technology Transfer 1995 is intended to inform the US industrial and academic sectors about the many opportunities they have to form partnerships with the US Department of Energy (DOE) for the mutual advantage of the individual institutions, DOE, and the nation as a whole. It also describes some of the growing number of remarkable achievements resulting from such partnerships. These partnership success stories offer ample evidence that Americans are learning how to work together to secure major benefits for the nation--by combining the technological, scientific, and human resources resident in national laboratories with those in industry and academia. The benefits include more and better jobs for Americans, improved productivity and global competitiveness for technology-based industries, and a more efficient government laboratory system.

  9. An intercalation-locked parallel-stranded DNA tetraplex

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Tripathi, S.; Zhang, D.; Paukstelis, P. J.

    2015-01-27

    DNA has proved to be an excellent material for nanoscale construction because complementary DNA duplexes are programmable and structurally predictable. However, in the absence of Watson–Crick pairings, DNA can be structurally more diverse. Here, we describe the crystal structures of d(ACTCGGATGAT) and the brominated derivative, d(ACBrUCGGABrUGAT). These oligonucleotides form parallel-stranded duplexes with a crystallographically equivalent strand, resulting in the first examples of DNA crystal structures that contains four different symmetric homo base pairs. Two of the parallel-stranded duplexes are coaxially stacked in opposite directions and locked together to form a tetraplex through intercalation of the 5'-most A–A base pairs betweenmore »adjacent G–G pairs in the partner duplex. The intercalation region is a new type of DNA tertiary structural motif with similarities to the i-motif. 1H–1H nuclear magnetic resonance and native gel electrophoresis confirmed the formation of a parallel-stranded duplex in solution. Finally, we modified specific nucleotide positions and added d(GAY) motifs to oligonucleotides and were readily able to obtain similar crystals. This suggests that this parallel-stranded DNA structure may be useful in the rational design of DNA crystals and nanostructures.« less

  10. Extreme bendability of DNA double helix due to bending asymmetry

    E-Print Network [OSTI]

    Hossein Salari; B. Eslami-Mossallam; M. S. Naderi; M. R. Ejtehadi

    2015-05-23

    Experimental data of the DNA cyclization (J-factor) at short length scales, as a way to study the elastic behavior of tightly bent DNA, exceed the theoretical expectation based on the wormlike chain (WLC) model by several orders of magnitude. Here, we propose that asymmetric bending rigidity of the double helix in the groove direction can be responsible for extreme bendability of DNA at short length scales and it also facilitates DNA loop formation at these lengths. To account for the bending asymmetry, we consider the asymmetric elastic rod (AER) model which has been introduced and parametrized in an earlier study (B. Eslami-Mossallam and M. Ejtehadi, Phys. Rev. E 80, 011919 (2009)). Exploiting a coarse grained representation of DNA molecule at base pair (bp) level, and using the Monte Carlo simulation method in combination with the umbrella sampling technique, we calculate the loop formation probability of DNA in the AER model. We show that, for DNA molecule has a larger J-factor compared to the WLC model which is in excellent agreement with recent experimental data.

  11. Osmotic pressure: resisting or promoting DNA ejection from phage

    E-Print Network [OSTI]

    Meerim Jeembaeva; Martin Castelnovo; Frida Larsson; Alex Evilevitch

    2008-06-24

    Recent in vitro experiments have shown that DNA ejection from bacteriophage can be partially stopped by surrounding osmotic pressure when ejected DNA is digested by DNase I on the course of ejection. We argue in this work by combination of experimental techniques (osmotic suppression without DNaseI monitored by UV absorbance, pulse-field electrophoresis, and cryo-EM visualization) and simple scaling modeling that intact genome (i.e. undigested) ejection in a crowded environment is, on the contrary, enhanced or eventually complete with the help of a pulling force resulting from DNA condensation induced by the osmotic stress itself. This demonstrates that in vivo, the osmotically stressed cell cytoplasm will promote phage DNA ejection rather than resisting it. The further addition of DNA-binding proteins under crowding conditions is shown to enhance the extent of ejection. We also found some optimal crowding conditions for which DNA content remaining in the capsid upon ejection is maximum, which correlates well with the optimal conditions of maximum DNA packaging efficiency into viral capsids observed almost 20 years ago. Biological consequences of this finding are discussed.

  12. Extreme bendability of DNA double helix due to bending asymmetry

    E-Print Network [OSTI]

    Salari, Hossein; Naderi, M S; Ejtehadi, M R

    2015-01-01

    Experimental data of the DNA cyclization (J-factor) at short length scales, as a way to study the elastic behavior of tightly bent DNA, exceed the theoretical expectation based on the wormlike chain (WLC) model by several orders of magnitude. Here, we propose that asymmetric bending rigidity of the double helix in the groove direction can be responsible for extreme bendability of DNA at short length scales and it also facilitates DNA loop formation at these lengths. To account for the bending asymmetry, we consider the asymmetric elastic rod (AER) model which has been introduced and parametrized in an earlier study (B. Eslami-Mossallam and M. Ejtehadi, Phys. Rev. E 80, 011919 (2009)). Exploiting a coarse grained representation of DNA molecule at base pair (bp) level, and using the Monte Carlo simulation method in combination with the umbrella sampling technique, we calculate the loop formation probability of DNA in the AER model. We show that, for DNA molecule has a larger J-factor compared to the WLC model w...

  13. Specificity, flexibility and valence of DNA bonds guide emulsion architecture

    E-Print Network [OSTI]

    Lang Feng; Lea-Laetitia Pontani; Remi Dreyfus; Paul Chaikin; Jasna Brujic

    2013-03-25

    The specificity and thermal reversibility of DNA interactions have enabled the self-assembly of crystal structures, self-replicating materials and colloidal molecules. Grafting DNA onto liquid interfaces of emulsions leads to exciting new architectural possibilities due to the mobility of the DNA ligands and the patches they form between bound droplets. Here we show that the size and number of these adhesion patches (valency) can be controlled. Valence 2 leads to flexible polymers of emulsion droplets, while valence above 4 leads to rigid droplet networks. A simple thermodynamic model quantitatively describes the increase in the patch size with droplet radii, DNA concentration and the stiffness of the tether to the sticky-end. The patches are formed between droplets with complementary DNA strands or alternatively with complementary colloidal nanoparticles to mediate DNA binding between droplets. This emulsion system opens the route to directed self-assembly of more complex structures through distinct DNA bonds with varying strengths and controlled valence and flexibility.

  14. State Technologies Advancement Collaborative

    SciTech Connect (OSTI)

    David S. Terry

    2012-01-30

    The U. S. Department of Energy (DOE), National Association of State Energy Officials (NASEO), and Association of State Energy Research and Technology Transfer Institutions (ASERTTI) signed an intergovernmental agreement on November 14, 2002, that allowed states and territories and the Federal Government to better collaborate on energy research, development, demonstration and deployment (RDD&D) projects. The agreement established the State Technologies Advancement Collaborative (STAC) which allowed the states and DOE to move RDD&D forward using an innovative competitive project selection and funding process. A cooperative agreement between DOE and NASEO served as the contracting instrument for this innovative federal-state partnership obligating funds from DOE's Office of Energy Efficiency and Renewable Energy and Office of Fossil Energy to plan, fund, and implement RDD&D projects that were consistent with the common priorities of the states and DOE. DOE's Golden Field Office provided Federal oversight and guidance for the STAC cooperative agreement. The STAC program was built on the foundation of prior Federal-State efforts to collaborate on and engage in joint planning for RDD&D. Although STAC builds on existing, successful programs, it is important to note that it was not intended to replace other successful joint DOE/State initiatives such as the State Energy Program or EERE Special Projects. Overall the STAC process was used to fund, through three competitive solicitations, 35 successful multi-state research, development, deployment, and demonstration projects with an overall average non-federal cost share of 43%. Twenty-two states were awarded at least one prime contract, and organizations in all 50 states and some territories were involved as subcontractors in at least one STAC project. Projects were funded in seven program areas: (1) Building Technologies, (2) Industrial Technologies, (3) Transportation Technologies, (4) Distributed Energy Resources, (5) Hydrogen Technology Learning Centers, (6) Fossil Energy, and (7) Rebuild America.

  15. IMPACCT: Carbon Capture Technology

    SciTech Connect (OSTI)

    2012-01-01

    IMPACCT Project: IMPACCT’s 15 projects seek to develop technologies for existing coal-fired power plants that will lower the cost of carbon capture. Short for “Innovative Materials and Processes for Advanced Carbon Capture Technologies,” the IMPACCT Project is geared toward minimizing the cost of removing carbon dioxide (CO2) from coal-fired power plant exhaust by developing materials and processes that have never before been considered for this application. Retrofitting coal-fired power plants to capture the CO2 they produce would enable greenhouse gas reductions without forcing these plants to close, shifting away from the inexpensive and abundant U.S. coal supply.

  16. Transformational Energy Technologies

    SciTech Connect (OSTI)

    None

    2010-09-01

    Broad Funding Opportunity Announcement Project: In 2009, ARPA-E issued an open call for the most revolutionary energy technologies to form the agency’s inaugural program. The first open solicitation was open to ideas from all energy areas and focused on funding projects already equipped with strong research and development plans for their potentially high-impact technologies. The 37 projects chosen received a level of financial support that could accelerate technical progress and catalyze additional investment from the private sector. After only 2 months, ARPA-E’s investment in these projects catalyzed an additional $33 million in investments.

  17. Roof bolting equipment & technology

    SciTech Connect (OSTI)

    Fiscor, S.

    2009-04-15

    Technology provides an evaluator path to improvement for roof bolting machines. Bucyrus offers three different roof bolts models for various mining conditions. The LRB-15 AR is a single-arm boiler recommended for ranges of 32 inches and above; the dual-arm RB2-52A for ranges of 42 inches and above; and the dual-arm RB2-88A for ranges of 54 inches and above. Design features are discussed in the article. Developments in roof bolting technology by Joy Mining Machinery are reported. 4 photos.

  18. Energy and Technology Review

    SciTech Connect (OSTI)

    Bookless, W.A.; Quirk, W.J.

    1994-06-01

    This report discusses: The Clementine satellite, the first US satellite to the Moon in more than two decades, sent back more than 1.5 million images of the lunar surface using cameras designed and calibrated by LLNL. An LLNL-developed laser ranger provided information that will be used to construct a relief map of the Moon`s surface; and Uncertainty and the Federal Role in Science and Technology, Ralph E. Gomory was a recent participate in the Director`s Distinguished Lecturer Series at LLNL. In his lecture, he addressed some of the tensions, conflicts, and possible goals related to federal support for science and technology.

  19. Energy and technology review

    SciTech Connect (OSTI)

    Stowers, I.F.; Crawford, R.B.; Esser, M.A.; Lien, P.L.; O'Neal, E.; Van Dyke, P.

    1982-07-01

    The state of the laboratory address by LLNL Director Roger Batzel is summarized, and a breakdown of the laboratory funding is given. The Livermore defense-related committment is described, including the design and development of advanced nuclear weapons as well as research in inertial confinement fusion, nonnuclear ordnance, and particle beam technology. LLNL is also applying its scientific and engineering resources to the dual challenge of meeting future energy needs without degrading the quality of the biosphere. Some representative examples are given of the supporting groups vital for providing the specialized expertise and new technologies required by the laboratory's major research programs. (GHT)

  20. Direct Conversion Technology

    SciTech Connect (OSTI)

    Back, L.H.; Fabris, G.; Ryan, M.A.

    1992-07-01

    The overall objective of the Direct Conversion Technology task is to develop an experimentally verified technology base for promising direct conversion systems that have potential application for energy conservation in the end-use sectors. Initially, two systems were selected for exploratory research and advanced development. These are Alkali Metal Thermal-to-Electric Converter (AMTEC) and Two-Phase Liquid Metal MD Generator (LMMHD). This report describes progress that has been made during the first six months of 1992 on research activities associated with these two systems. (GHH)