Sample records for dna ormat technologies

  1. Ormat Technologies Inc. Ormat Technologies Reports 2012 Fourth...

    Open Energy Info (EERE)

    Ormat Technologies Inc. Ormat Technologies Reports 2012 Fourth Quarter and Year End Results Jump to: navigation, search OpenEI Reference LibraryAdd to library Web Site: Ormat...

  2. Ormat Technologies Inc. Ormat Technologies, Inc. Announces a...

    Open Energy Info (EERE)

    Technologies, Inc. Announces a Non-Cash Pre-Tax Charge for Impairment to Its North Brawley Geothermal Power Plant Jump to: navigation, search OpenEI Reference LibraryAdd to library...

  3. Ormat Technologies Inc | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directedAnnual SiteofEvaluatingGroup |JilinLuOpenNorthOlympia GreenThesource History ViewOrmat Technologies

  4. Glass Buttes Exploration and Drilling: 2010 Geothermal Technologies Program Peer Review Presentation, Walsh, et al, Ormat

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Zemach, Ezra

    Glass Buttes Exploration and Drilling: 2010 Geothermal Technologies Program Peer Review Presentation, Walsh, et al, Ormat

  5. Glass Buttes Exploration and Drilling: 2010 Geothermal Technologies Program Peer Review Presentation, Walsh, et al, Ormat

    SciTech Connect (OSTI)

    Zemach, Ezra

    2010-01-01T23:59:59.000Z

    Glass Buttes Exploration and Drilling: 2010 Geothermal Technologies Program Peer Review Presentation, Walsh, et al, Ormat

  6. EA-1944: Ormat Technologies Brady Hot Springs Project, Churchill County, NV

    Broader source: Energy.gov [DOE]

    Ormat Technologies, Inc. (Ormat) proposes to use DOE and cost share funding to study the Brady Hot Springs geothermal Field 15-12RD well. This is an EGS Demonstration project divided into three phases. During Phase 1, Ormat characterized the target well to prepare for stimulation activities in Phase 2, Phase 2: Well Stimulation and Collection/Analysis of Stimulation Monitoring Data and Phase 3: Long-term testing of the system. Phase 2 and 3 activities would occur at Ormat's Brady Hot Springs geothermal field in Churchill County, NV on public lands managed by the Bureau of Land Management (BLM) and Bureau of Reclamation (BOR). Since Phases 2 and 3 have the potential to impact subsurface resources, DOE must analyze the impacts associated with Phases 2 and 3. The BLM will be the lead agency for completion of the EA with BOR and DOE as cooperating agencies.

  7. EA-1849: Ormat Nevada Geothermal Projects in Northern NV | Department...

    Office of Environmental Management (EM)

    9: Ormat Nevada Geothermal Projects in Northern NV EA-1849: Ormat Nevada Geothermal Projects in Northern NV August 22, 2011 EA-1849: Final Environmental Assessment Tuscarora...

  8. Energy Department Finalizes Loan Guarantee for Ormat Geothermal...

    Energy Savers [EERE]

    Loan Guarantee for Ormat Geothermal Project in Nevada Energy Department Finalizes Loan Guarantee for Ormat Geothermal Project in Nevada September 23, 2011 - 3:37pm Addthis...

  9. Ormat Technologies Inc. Ormat Technologies Reports 2012 Fourth Quarter and

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere I Geothermal Pwer PlantMunhall,Missouri:Energy InformationOregon: EnergyOrlovista,

  10. Josh Nordquist Director Ormat Technologies, Inc. DOE's ...

    Office of Environmental Management (EM)

    These compressor stations mainly use natural gas from the pipeline as fuel for the compressors, which are mostly gas turbines or gas engines. The waste heat from these...

  11. Ormat Technologies Inc | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to: navigation, searchOfRoseConcernsCompany OilInformation

  12. Ormat Technologies Inc | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere I Geothermal Pwer PlantMunhall,Missouri:Energy InformationOregon: EnergyOrlovista, Florida:

  13. EA-1849: Department of Energy Loan Guarantee to Ormat Nevada, Inc. for a Geothermal Power Facility in Nevada

    Broader source: Energy.gov [DOE]

    Ormat Nevada Inc. (ORMAT), through its subsidiaries, proposes to construct and operate three geothermal power production facilities and associated power transmission lines in northern Nevada. The...

  14. Ormat Technologies Inc. Ormat Technologies, Inc. Announces a Non-Cash

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to: navigation, searchOfRoseConcernsCompany OilInformationPre-Tax Charge for Impairment to

  15. EA-1849-S1: Phase II Facility- Ormat Tuscarora Geothermal Power Plant in Tuscarora, NV

    Broader source: Energy.gov [DOE]

    This Supplemental Environmental Assessment (SEA) will evaluate the potential impacts of the Phase II Facility of the Ormat Tuscarora Geothermal Power Plant.

  16. Ormat Technologies Inc. North Brawley, California USA | Open Energy

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere I Geothermal Pwer PlantMunhall,Missouri:Energy InformationOregon: EnergyOrlovista, Florida:Information

  17. BLM Fact Sheet- Ormat Technologies Salt Wells Geothermal Energy Project |

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directedAnnualProperty EditCalifornia: EnergyAvignon, France: Energy ResourcesBurley Field Office

  18. Assembling semiconductor nanocomposites using DNA replication technologies.

    SciTech Connect (OSTI)

    Heimer, Brandon W.; Crown, Kevin K.; Bachand, George David

    2005-11-01T23:59:59.000Z

    Deoxyribonucleic acid (DNA) molecules represent Nature's genetic database, encoding the information necessary for all cellular processes. From a materials engineering perspective, DNA represents a nanoscale scaffold with highly refined structure, stability across a wide range of environmental conditions, and the ability to interact with a range of biomolecules. The ability to mass-manufacture functionalized DNA strands with Angstrom-level resolution through DNA replication technology, however, has not been explored. The long-term goal of the work presented in this report is focused on exploiting DNA and in vitro DNA replication processes to mass-manufacture nanocomposite materials. The specific objectives of this project were to: (1) develop methods for replicating DNA strands that incorporate nucleotides with ''chemical handles'', and (2) demonstrate attachment of nanocrystal quantum dots (nQDs) to functionalized DNA strands. Polymerase chain reaction (PCR) and primer extension methodologies were used to successfully synthesize amine-, thiol-, and biotin-functionalized DNA molecules. Significant variability in the efficiency of modified nucleotide incorporation was observed, and attributed to the intrinsic properties of the modified nucleotides. Noncovalent attachment of streptavidin-coated nQDs to biotin-modified DNA synthesized using the primer extension method was observed by epifluorescence microscopy. Data regarding covalent attachment of nQDs to amine- and thiol-functionalized DNA was generally inconclusive; alternative characterization tools are necessary to fully evaluate these attachment methods. Full realization of this technology may facilitate new approaches to manufacturing materials at the nanoscale. In addition, composite nQD-DNA materials may serve as novel recognition elements in sensor devices, or be used as diagnostic tools for forensic analyses. This report summarizes the results obtained over the course of this 1-year project.

  19. Vehicle Technologies Office Merit Review 2014: Fleet DNA

    Broader source: Energy.gov [DOE]

    Presentation given by National Renewable Energy Laboratory at 2014 DOE Hydrogen and Fuel Cells Program and Vehicle Technologies Office Annual Merit Review and Peer Evaluation Meeting about fleet DNA.

  20. DNA . DNA

    E-Print Network [OSTI]

    1. DNA . , . , . . DNA DNA . , DNA . DNA . DNA . DNA DNA DNA . DNA [6, 7, 8]. DNA . DNA NACST/Sim DNA/DNA

  1. DNA DNA DNA (d)DNA DNA DNA

    E-Print Network [OSTI]

    Hagiya, Masami

    DNA DNA DNA DNA DNA DNA DNA DNA [ 2008] (d)DNA DNA DNA DNA 2 3 DNA DNA DNA DNA DNA DNA DNA (a) (c) (b) (d) #12;DNA DNA DNA DNA DNA DNA DNA DNA (b) DNA [Tanaka et al.2008] DNA DNA DNA DNA DNA DNA DNA #12;iGEM MIT MIT

  2. Sub-Lithographic Patterning Technology for Nanowire Model Catalysts and DNA Label-Free Hybridization Detection

    E-Print Network [OSTI]

    Bokor, Jeffrey

    Sub-Lithographic Patterning Technology for Nanowire Model Catalysts and DNA Label were used as a mold in nanoimprint lithography and lift-off patterning of sub-30nm platinum nanowires a label-free tool for DNA hybridization detection based on measuring capacitance changes in the gap

  3. Energy and Technology Review: Unlocking the mysteries of DNA repair

    SciTech Connect (OSTI)

    Quirk, W.A.

    1993-04-01T23:59:59.000Z

    DNA, the genetic blueprint, has the remarkable property of encoding its own repair following diverse types of structural damage induced by external agents or normal metabolism. We are studying the interplay of DNA damaging agents, repair genes, and their protein products to decipher the complex biochemical pathways that mediate such repair. Our research focuses on repair processes that correct DNA damage produced by chemical mutagens and radiation, both ionizing and ultraviolet. The most important type of DNA repair in human cells is called excision repair. This multistep process removes damaged or inappropriate pieces of DNA -- often as a string of 29 nucleotides containing the damage -- and replaces them with intact ones. We have isolated, cloned, and mapped several human repair genes associated with the nucleotide excision repair pathway and involved in the repair of DNA damage after exposure to ultraviolet light or mutagens in cooked food. We have shown that a defect in one of these repair genes, ERCC2, is responsible for the repair deficiency in one of the groups of patients with the recessive genetic disorder xeroderma pigmentosum (XP group D). We are exploring ways to purify sufficient quantities (milligrams) of the protein products of these and other repair genes so that we can understand their functions. Our long-term goals are to link defective repair proteins to human DNA repair disorders that predispose to cancer, and to produce DNA-repair-deficient mice that can serve as models for the human disorders.

  4. DNA DNA . DNA

    E-Print Network [OSTI]

    1. DNA DNA , . . DNA ( ) DNA "exhaustive " . Boolean , 40 2 40 10 12 pico mole . DNA . 20 3-SAT DNA NP-complete [1]. [2, 3]. DNA

  5. DNA Microarray Technologies: A Novel Approach to Geonomic Research

    SciTech Connect (OSTI)

    Hinman, R.; Thrall, B.; Wong, K,

    2002-01-01T23:59:59.000Z

    A cDNA microarray allows biologists to examine the expression of thousands of genes simultaneously. Researchers may analyze the complete transcriptional program of an organism in response to specific physiological or developmental conditions. By design, a cDNA microarray is an experiment with many variables and few controls. One question that inevitably arises when working with a cDNA microarray is data reproducibility. How easy is it to confirm mRNA expression patterns? In this paper, a case study involving the treatment of a murine macrophage RAW 264.7 cell line with tumor necrosis factor alpha (TNF) was used to obtain a rough estimate of data reproducibility. Two trials were examined and a list of genes displaying either a > 2-fold or > 4-fold increase in gene expression was compiled. Variations in signal mean ratios between the two slides were observed. We can assume that erring in reproducibility may be compensated by greater inductive levels of similar genes. Steps taken to obtain results included serum starvation of cells before treatment, tests of mRNA for quality/consistency, and data normalization.

  6. Vehicle Technologies Office Merit Review 2013: Fleet DNA

    Broader source: Energy.gov [DOE]

    Presentation given by the National Renewable Energy Laboratory (NREL) at the 2013 DOE Hydrogen and Fuel Cells Program and Vehicle Technologies Program Annual Merit Review and Peer Evaluation Meeting about a tool for analyzing fleet characteristics.

  7. DNA -DNA--

    E-Print Network [OSTI]

    Glykos, Nikolaos

    #12;#12;#12;8.1 DNA - . DNA- - (DNA-binding domains) ( 100 ). - DNA- - -, DNA. - -- - DNA. 173 8 DNA : -- 8.1 8.2 8.3 Cro - 8.4 Cro 8.5 DNA- - 8.6 Cro DN 8

  8. ORMAT NEVADA | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page onYouTube YouTube Note: Since the.pdfBreaking ofOilNEWResponse to Time-BasedDecember 23,Misc Cases TOREM renews

  9. . DNA, RNA, Protein

    E-Print Network [OSTI]

    Yoo, SukIn

    1. , . DNA, RNA, Protein [1-4]. DNA . DNA A, T, G, C . DNA , DNA DNA . , DNA DNA . DNA DNA . , PCR , , DNA [5]. DNA DNA , DNA

  10. Vehicle Technologies Office Merit Review 2015: Fleet DNA Phase 1 Refinement & Phase 2 Implementation

    Broader source: Energy.gov [DOE]

    Presentation given by National Renewable Energy Laboratory at 2015 DOE Hydrogen and Fuel Cells Program and Vehicle Technologies Office Annual Merit Review and Peer Evaluation Meeting about Fleet...

  11. Quantitative DNA fiber mapping

    DOE Patents [OSTI]

    Gray, Joe W. (San Francisco, CA); Weier, Heinz-Ulrich G. (Oakland, CA)

    1998-01-01T23:59:59.000Z

    The present invention relates generally to the DNA mapping and sequencing technologies. In particular, the present invention provides enhanced methods and compositions for the physical mapping and positional cloning of genomic DNA. The present invention also provides a useful analytical technique to directly map cloned DNA sequences onto individual stretched DNA molecules.

  12. GenTegra DNA Tubes is another proprietary technology for stor-ing purified DNA in a `bone-dry', water-free environment. This new

    E-Print Network [OSTI]

    Cai, Long

    -dry', water-free environment. This new matrix protects DNA samples from hydrolysis and oxidation platforms, including Illumina and Affymetrix. Dry-state, room-temperature storage of DNA and RNA Water, Heather E McMahon & Michael E Hogan GenVault Corporation, 6190 Corte Del Cedro, Carlsbad, California 92011

  13. Now available at the DNA Sequencing and Genotyping Core Facility OpenArray High-throughput nanofluidic PCR technology

    E-Print Network [OSTI]

    Ruvinsky, Ilya

    -throughput nanofluidic PCR technology from Life Technologies/Applied Biosystems Application/ Service Area Types · Experimental flexibility ­ open-format layout · Nanofluidic design reduces reagent usage Questions? Contact

  14. Ormat Nevada, Inc. | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    will convert underground heat into electrical energy by utilizing heated fluid, or brine, which is extracted from deep within the Earth's crust. Brine, heated around 330F,...

  15. Ormat Funding Corp | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere I Geothermal Pwer PlantMunhall,Missouri:Energy InformationOregon: EnergyOrlovista, Florida: EnergyFunding

  16. Chimeric proteins for detection and quantitation of DNA mutations, DNA sequence variations, DNA damage and DNA mismatches

    DOE Patents [OSTI]

    McCutchen-Maloney, Sandra L. (Pleasanton, CA)

    2002-01-01T23:59:59.000Z

    Chimeric proteins having both DNA mutation binding activity and nuclease activity are synthesized by recombinant technology. The proteins are of the general formula A-L-B and B-L-A where A is a peptide having DNA mutation binding activity, L is a linker and B is a peptide having nuclease activity. The chimeric proteins are useful for detection and identification of DNA sequence variations including DNA mutations (including DNA damage and mismatches) by binding to the DNA mutation and cutting the DNA once the DNA mutation is detected.

  17. Nanotechnology with DNA DNA Nanodevices

    E-Print Network [OSTI]

    Ludwig-Maximilians-Universität, München

    Nanotechnology with DNA DNA Nanodevices Friedrich C. Simmel* and Wendy U. Dittmer A DNA actuator. Introduction.............285 2. Overview: DNA Nanotechnology.......285 3. Prototypes of Nanomechanical DNA overview of DNA nanotechnology as a whole is given. The most important properties of DNA molecules

  18. DNA microarray (spot) .

    E-Print Network [OSTI]

    1. DNA microarray DNA (spot) . DNA probe , probe (hybridization) . DNA microarray cDNA oligonucleotide oligonucleotide cDNA probe . oligonucleotide microarray , DNA , probe . oligonucleotide microarray probe

  19. Technology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of ScienceandMesa del SolStrengthening a solidSynthesis of 2D AlloysTrails NewsTechnologyTechnology A

  20. Technology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of ScienceandMesa del SolStrengthening a solidSynthesis of 2D AlloysTrails NewsTechnologyTechnology

  1. DNA Computing Hamiltonian path

    E-Print Network [OSTI]

    Hagiya, Masami

    2014 DNA DNA #12;DNA Computing · Feynman · Adleman · DNASIMD · ... · · · · · DNADNA #12;DNA · DNA · · · · DNA · · #12;2000 2005 2010 1995 Hamiltonian path DNA tweezers DNA tile DNA origami DNA box Sierpinski DNA tile self assembly DNA logic gates Whiplash PCR DNA automaton DNA spider MAYA

  2. Single molecule analysis of DNA electrophoresis in microdevices

    E-Print Network [OSTI]

    Randall, Greg C

    2006-01-01T23:59:59.000Z

    Given that current electrophoresis technology is inadequate for mapping large O[100 kilobasepair] DNA, several promising lab-on-chip designs for DNA mapping have been recently proposed that require either 1) a DNA molecule ...

  3. DNA Copyright

    E-Print Network [OSTI]

    Torrance, Andrew W.

    2011-01-01T23:59:59.000Z

    of architecture and computer software. Sequences of DNA should also be acknowledged as eligible for copyright protection. Unaltered genomic DNA sequences would seem poor candidates for copyright protection. The case is stronger for copyright protection...

  4. DNA demethylation by DNA repair

    E-Print Network [OSTI]

    Gehring, Mary

    Active DNA demethylation underlies key facets of reproduction in flowering plants and mammals and serves a general genome housekeeping function in plants. A family of 5-methylcytosine DNA glycosylases catalyzes plant ...

  5. Geothermal News | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    May 16, 2013 Nevada Deploys Grid-Connected Electricity from Enhanced Geothermal Systems Ormat Technologies develops first commercial EGS project to supply electricity to the grid....

  6. Integrated Approach to Use Natural Chemical and Isotopic Tracers...

    Broader source: Energy.gov (indexed) [DOE]

    * Reservoir Sustainability - Barrier M: Long-term sustainability - Partners: NA - Acknowledgement: Ormat Technologies, Inc. (core samples) Project Overview 3 | US DOE Geothermal...

  7. Merging high resolution geophysical and geochemical surveys to...

    Broader source: Energy.gov (indexed) [DOE]

    Merging high resolution geophysical and geochemical surveys to reduce exploration risk at Glass Buttes, Oregon Patrick Walsh Ormat Nevada Inc. Innovative technologies May 19, 2010...

  8. DNA Pendant

    E-Print Network [OSTI]

    Hacker, Randi; Tsutsui, William

    2007-11-14T23:59:59.000Z

    Broadcast Transcript: It's a symbol of commitment. It's a memento mori. It's the DNA pendant offered by Japan's Eiwa Industry and it's two, two, two things in one. Using genetic extraction, Eiwa removes the DNA from, say, a strand of hair or a...

  9. Fleet DNA (Presentation)

    SciTech Connect (OSTI)

    Walkokwicz, K.; Duran, A.

    2014-06-01T23:59:59.000Z

    The Fleet DNA project objectives include capturing and quantifying drive cycle and technology variation for the multitude of medium- and heavy-duty vocations; providing a common data storage warehouse for medium- and heavy-duty vehicle fleet data across DOE activities and laboratories; and integrating existing DOE tools, models, and analyses to provide data-driven decision making capabilities. Fleet DNA advantages include: for Government - providing in-use data for standard drive cycle development, R&D, tech targets, and rule making; for OEMs - real-world usage datasets provide concrete examples of customer use profiles; for fleets - vocational datasets help illustrate how to maximize return on technology investments; for Funding Agencies - ways are revealed to optimize the impact of financial incentive offers; and for researchers -a data source is provided for modeling and simulation.

  10. Mitigating security issues in the evolving DNA synthesis industry

    E-Print Network [OSTI]

    Turlington, Ralph Donald, III

    2013-01-01T23:59:59.000Z

    DNA synthesis technologies are advancing at exponential rates, with production of ever longer, more complex, and less expensive sequences of double stranded DNA. This has fostered development of industrial scale design, ...

  11. Energy Department Finalizes Loan Guarantee for Ormat Geothermal Project in

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page onYouTube YouTube Note: Since the YouTube|6721 Federal Register /ofConcentratingDepartment offor Innovative

  12. DNA Computing Complexity Analysis Using DNA/DNA Hybridization Kinetics

    E-Print Network [OSTI]

    DNA Computing Complexity Analysis Using DNA/DNA Hybridization Kinetics Soo­Yong Shin 1 , Eun Jeong the complexity of DNA computing. The complexity of any computational algorithm is typically measured in terms of time and space. In DNA computing, the time complexity can be measured by the total reaction time

  13. DNA Computing Complexity Analysis Using DNA/DNA Hybridization Kinetics

    E-Print Network [OSTI]

    DNA Computing Complexity Analysis Using DNA/DNA Hybridization Kinetics Soo-Yong Shin1 , Eun Jeong of DNA computing. The complexity of any computational algorithm is typically measured in terms of time and space. In DNA computing, the time complexity can be measured by the total reaction time

  14. (gene expression) DNA (DNA microarrays).

    E-Print Network [OSTI]

    Athens, University of

    Lymphoblastic Leukemia - ALL, 25 Acute Myeloid Leukemia - AML) µ 7129 [10]. µ µ µ µ µ µ µ µ DNA. 62 µ, 22 40 , µ 2000 [6]. µ 72 µ µ (47 Acute

  15. ##### SAT Engine ####### _ ############ DNA ###### _

    E-Print Network [OSTI]

    Hagiya, Masami

    ##### SAT Engine ####### _ ############ DNA ###### _ # # # #y # # #yy # # # #yy ###### DNA #################################### ############### ##################### 6 ## 10 ##### ### DNA ############### (Sakamoto et al., Science, Vol.288, pp.1223-122* *6

  16. Design and Characterization of Programmable DNA Nanotubes Supporting Information

    E-Print Network [OSTI]

    Fygenson, Deborah Kuchnir

    . DNA sample preparation: Lyophilized HPLC- or PAGE- purified DNA oligonucleotides were purchased from Integrated DNA Technologies (Coralville, IA), resuspended in water, quanti- tated by UV absorbance at 260 nm immersion and 40X/0.75 NA air objectives. Blue light was filtered from a mer- cury arc lamp thro

  17. Adleman[1] 1994 DNA Hamiltonian Path Problem , DNA

    E-Print Network [OSTI]

    1. Adleman[1] 1994 DNA Hamiltonian Path Problem , DNA DNA [2]. DNA DNA , . , , 2 , DNA 4 . DNA 4 A(Adenine), C(Cytosine), G(Guanine), T(Thymine) 2 4 . , . 1 mole 6x10 23 DNA DNA . , . DNA NP-complete [1, 2], [2

  18. DNA Topology: Fundamentals

    E-Print Network [OSTI]

    Mirkin, Sergei

    DNA Topology: Fundamentals Sergei M Mirkin, University of Illinois at Chicago, Illinois, USA Topological characteristics of DNA and specifically DNA supercoiling influence all major DNA transactions in living cells. DNA supercoiling induces the formation of unusual secondary structure by specific DNA

  19. Storing data encoded DNA in living organisms

    DOE Patents [OSTI]

    Wong; Pak C. (Richland, WA), Wong; Kwong K. (Sugar Land, TX), Foote; Harlan P. (Richland, WA)

    2006-06-06T23:59:59.000Z

    Current technologies allow the generation of artificial DNA molecules and/or the ability to alter the DNA sequences of existing DNA molecules. With a careful coding scheme and arrangement, it is possible to encode important information as an artificial DNA strand and store it in a living host safely and permanently. This inventive technology can be used to identify origins and protect R&D investments. It can also be used in environmental research to track generations of organisms and observe the ecological impact of pollutants. Today, there are microorganisms that can survive under extreme conditions. As well, it is advantageous to consider multicellular organisms as hosts for stored information. These living organisms can provide as memory housing and protection for stored data or information. The present invention provides well for data storage in a living organism wherein at least one DNA sequence is encoded to represent data and incorporated into a living organism.

  20. Fleet DNA Project (Fact Sheet)

    SciTech Connect (OSTI)

    Not Available

    2012-10-01T23:59:59.000Z

    The Fleet DNA Project - designed by the U.S. Department of Energy's National Renewable Energy Laboratory (NREL) in partnership with Oak Ridge National Laboratory - aims to accelerate the evolution of advanced vehicle development and support the strategic deployment of market-ready technologies that reduce costs, fuel consumption, and emissions. At the heart of the Fleet DNA Project is a clearinghouse of medium- and heavy-duty commercial fleet transportation data for optimizing the design of advanced vehicle technologies or for selecting a given technology to invest in. An easy-to-access online database will help vehicle manufacturers and fleets understand the broad operational range for many of today's commercial vehicle vocations.

  1. National Account Energy Alliance Final Report for the Basin Electric Project at Northern Border Pipeline Company's Compressor Station #7, North Dakota

    SciTech Connect (OSTI)

    Sweetzer, Richard [Exergy Partners Corp.; Leslie, Neil [Gas Technology Institute

    2008-02-01T23:59:59.000Z

    A field research test and verification project was conducted at the recovered energy generation plant at Northern Border Pipeline Company Compressor Station #7 (CS#7) near St. Anthony. Recovered energy generation plant equipment was supplied and installed by ORMAT Technologies, Inc. Basin Electric is purchasing the electricity under a purchase power agreement with an ORMAT subsidiary, which owns and operates the plant.

  2. DNA polymerase with modified processivity

    DOE Patents [OSTI]

    Bedford, Ella (Brookline, MA); Tabor, Stanley (Cambridge, MA); Richardson, Charles C. (Chestnut Hill, MA)

    1999-01-01T23:59:59.000Z

    Chimeric DNA polymerase having a DNA polymerase domain and processivity factor binding domain not naturally associated with DNA polymerase domain.

  3. DNA Extraction From Processed Wood: A Case Study for the Identification of an Endangered

    E-Print Network [OSTI]

    Protocols DNA Extraction From Processed Wood: A Case Study for the Identification of an Endangered to the extraction of whole genomic DNA from processed wood samples to explore the possibility of identifying an endangered trop- ical timber species by using DNA sequencing technology. High-yield and high-quality DNA

  4. Advance the DNA computing 

    E-Print Network [OSTI]

    Qiu, Zhiquan Frank

    2004-09-30T23:59:59.000Z

    DNA computer. The existing models from which a few DNA computing algorithms have been developed are not sufficiently powerful and robust, however, to attract potential users. This thesis has described research performed to build a new DNA computing...

  5. Synthesis of DNA

    DOE Patents [OSTI]

    Mariella, Jr., Raymond P. (Danville, CA)

    2008-11-18T23:59:59.000Z

    A method of synthesizing a desired double-stranded DNA of a predetermined length and of a predetermined sequence. Preselected sequence segments that will complete the desired double-stranded DNA are determined. Preselected segment sequences of DNA that will be used to complete the desired double-stranded DNA are provided. The preselected segment sequences of DNA are assembled to produce the desired double-stranded DNA.

  6. DNA encoding a DNA repair protein

    DOE Patents [OSTI]

    Petrini, John H.; Morgan, William Francis; Maser, Richard Scott; Carney, James Patrick

    2006-08-15T23:59:59.000Z

    An isolated and purified DNA molecule encoding a DNA repair protein, p95, is provided, as is isolated and purified p95. Also provided are methods of detecting p95 and DNA encoding p95. The invention further provides p95 knock-out mice.

  7. Bioinformatics challenges of new sequencing technology

    E-Print Network [OSTI]

    Pfrender, Michael

    Bioinformatics challenges of new sequencing technology Mihai Pop and Steven L. Salzberg Center for Bioinformatics and Computational Biology, University of Maryland, MD 20742, USA New DNA sequencing technologies a range of species using the new technologies. However, these new technologies produce read lengths

  8. ENGINEERING TECHNOLOGY Engineering Technology

    E-Print Network [OSTI]

    ENGINEERING TECHNOLOGY Engineering Technology Program The Bachelor of Science in Engineering Technology (BSET) is a hands-on program based upon engineering technology fundamentals, engineering for employment or further education. The focus is on current engineering technology issues and applications used

  9. Natural DNA sequencing by synthesis

    E-Print Network [OSTI]

    Roller, Eric E.

    2011-01-01T23:59:59.000Z

    labeled nucleotides by DNA polymerases. Biotechniques, 2005.S.A. , et al. , Multiplexed DNA sequencing-by-synthesis.Assembly of High-Density DNA Arrays for Genomic Analyses.

  10. Emerging technologies

    SciTech Connect (OSTI)

    Lu, Shin-yee

    1993-03-01T23:59:59.000Z

    The mission of the Emerging Technologies thrust area at Lawrence Livermore National Laboratory is to help individuals establish technology areas that have national and commercial impact, and are outside the scope of the existing thrust areas. We continue to encourage innovative ideas that bring quality results to existing programs. We also take as our mission the encouragement of investment in new technology areas that are important to the economic competitiveness of this nation. In fiscal year 1992, we have focused on nine projects, summarized in this report: (1) Tire, Accident, Handling, and Roadway Safety; (2) EXTRANSYT: An Expert System for Advanced Traffic Management; (3) Odin: A High-Power, Underwater, Acoustic Transmitter for Surveillance Applications; (4) Passive Seismic Reservoir Monitoring: Signal Processing Innovations; (5) Paste Extrudable Explosive Aft Charge for Multi-Stage Munitions; (6) A Continuum Model for Reinforced Concrete at High Pressures and Strain Rates: Interim Report; (7) Benchmarking of the Criticality Evaluation Code COG; (8) Fast Algorithm for Large-Scale Consensus DNA Sequence Assembly; and (9) Using Electrical Heating to Enhance the Extraction of Volatile Organic Compounds from Soil.

  11. Binary electrokinetic separation of target DNA from background DNA primers.

    SciTech Connect (OSTI)

    James, Conrad D.; Derzon, Mark Steven

    2005-10-01T23:59:59.000Z

    This report contains the summary of LDRD project 91312, titled ''Binary Electrokinetic Separation of Target DNA from Background DNA Primers''. This work is the first product of a collaboration with Columbia University and the Northeast BioDefense Center of Excellence. In conjunction with Ian Lipkin's lab, we are developing a technique to reduce false positive events, due to the detection of unhybridized reporter molecules, in a sensitive and multiplexed detection scheme for nucleic acids developed by the Lipkin lab. This is the most significant problem in the operation of their capability. As they are developing the tools for rapidly detecting the entire panel of hemorrhagic fevers this technology will immediately serve an important national need. The goal of this work was to attempt to separate nucleic acid from a preprocessed sample. We demonstrated the preconcentration of kilobase-pair length double-stranded DNA targets, and observed little preconcentration of 60 base-pair length single-stranded DNA probes. These objectives were accomplished in microdevice formats that are compatible with larger detection systems for sample pre-processing. Combined with Columbia's expertise, this technology would enable a unique, fast, and potentially compact method for detecting/identifying genetically-modified organisms and multiplexed rapid nucleic acid identification. Another competing approach is the DARPA funded IRIS Pharmaceutical TIGER platform which requires many hours for operation, and an 800k$ piece of equipment that fills a room. The Columbia/SNL system could provide a result in 30 minutes, at the cost of a few thousand dollars for the platform, and would be the size of a shoebox or smaller.

  12. DNA conjugation andDNA conjugation and reversibility onreversibility on

    E-Print Network [OSTI]

    Rubloff, Gary W.

    DNA conjugation andDNA conjugation and reversibility onreversibility on chitosan surfaceschitosan surfaceschitosan surfaceschitosan surfaces Rubloff Research Group Accomplishments #12;DNA conjugation and reversibility onDNA conjugation and reversibility on chitosan surfaceschitosan surfaces Accomplishment Single

  13. Meta-DNA: synthetic biology via DNA nanostructures and

    E-Print Network [OSTI]

    Reif, John H.

    Meta-DNA: synthetic biology via DNA nanostructures and hybridization reactions Harish Chandran1 of DNA manipulations achieved by protein enzymes be simulated via simple DNA hybridization chemistry? In this work, we develop a biochemical system which we call meta-DNA (abbreviated as mDNA), based on strands

  14. DNA fragment assembly: an application of graph theory in molecular biology

    E-Print Network [OSTI]

    Willems, Wolfgang

    DNA fragment assembly: an application of graph theory in molecular biology Martin Mascher Leibniz Technology Since the central importance of the DNA in storing biological informa- tion had been recognised limitations permit scientists only to obtain contigu- ous DNA fragments whose lengths range from a few dozen

  15. Quantitive DNA Fiber Mapping

    E-Print Network [OSTI]

    Lu, Chun-Mei

    2009-01-01T23:59:59.000Z

    of California. Lu et al. : DNA Fiber Mapping page - 35 Lu etal. : DNA Fiber Mapping page - 36 a b c d e f g OV P1 cloneSp6 end T7 end Lu et al. : DNA Fiber Mapping page - 37 a b c

  16. DNA Sequencing apparatus

    DOE Patents [OSTI]

    Tabor, Stanley (Cambridge, MA); Richardson, Charles C. (Chestnut Hill, MA)

    1992-01-01T23:59:59.000Z

    An automated DNA sequencing apparatus having a reactor for providing at least two series of DNA products formed from a single primer and a DNA strand, each DNA product of a series differing in molecular weight and having a chain terminating agent at one end; separating means for separating the DNA products to form a series bands, the intensity of substantially all nearby bands in a different series being different, band reading means for determining the position an This invention was made with government support including a grant from the U.S. Public Health Service, contract number AI-06045. The U.S. government has certain rights in the invention.

  17. DNA: structure, dense phases, charges, interactions

    E-Print Network [OSTI]

    Potsdam, Universität

    DNA: structure, dense phases, charges, interactions #12;Outline 1. DNA: structure, charges, dense phases 2. Counterion and DNA condensation 3. ES DNA-DNA interactions 4. DNA toroidal structures 5. Interactions of real DNA helices 6. DNA-DNA ES recognition 7. DNA melting in aggregates 8. Azimuthal

  18. Microsoft Word - MPW_Fact_Sheet_SAND2010-4820P_updated_format...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    SOI t ormation e holly owned ministration MPW service circuit desig n and makes enter and ISO eering suppo technology. www.s email snlas es for CMOS gns on a sing it more cost O...

  19. Detection and quantitation of single nucleotide polymorphisms, DNA sequence variations, DNA mutations, DNA damage and DNA mismatches

    SciTech Connect (OSTI)

    McCutchen-Maloney, Sandra L. (Pleasanton, CA)

    2002-01-01T23:59:59.000Z

    DNA mutation binding proteins alone and as chimeric proteins with nucleases are used with solid supports to detect DNA sequence variations, DNA mutations and single nucleotide polymorphisms. The solid supports may be flow cytometry beads, DNA chips, glass slides or DNA dips sticks. DNA molecules are coupled to solid supports to form DNA-support complexes. Labeled DNA is used with unlabeled DNA mutation binding proteins such at TthMutS to detect DNA sequence variations, DNA mutations and single nucleotide length polymorphisms by binding which gives an increase in signal. Unlabeled DNA is utilized with labeled chimeras to detect DNA sequence variations, DNA mutations and single nucleotide length polymorphisms by nuclease activity of the chimera which gives a decrease in signal.

  20. (2) DNA O(n^5) Quorum-Sensing Lux

    E-Print Network [OSTI]

    Hagiya, Masami

    - 1 - ( ) ( ) DNA RNA DNA RNA DNA DNA 2 DNA #12;- 2 - 17 6 (1) (2) DNA O(n^5) (3) Quorum-Sensing Lux (4) (5) LMNtal ambient LMNtal (1) (2) DNA (3) DNA (4) DNA (5) DNA (1) DNA ANP-96 (Precision System Science ) (2) RTRACS DNA RTRACS (3) in vivo in vivo (4) DNA trans cis 1/10 (5) DNA-PNA DNA DNA DNA DNA DNA

  1. Electrochemical DNA Hybridization Detection Using DNA Dohyoung Kwon,a

    E-Print Network [OSTI]

    Kwak, Juhyoun

    Full Paper Electrochemical DNA Hybridization Detection Using DNA Cleavage Dohyoung Kwon,a Kyuwon method for detection of DNA hybridization using enzymatic cleavage. The strategy is based on that S1 nuclease is able to specifically cleave only single strand DNA, but not double strand DNA. The capture

  2. Modeling DNA Interactions in Nucleosome Particles (A Computational Study of DNA Interactions in Nucleosome Particles)

    E-Print Network [OSTI]

    Kansas, University of

    Technology Center, University of Kansas, Lawrence, KS 66045. 2006 Annual Kansas City Area Life Sciences characterize global motions of the histone-DNA complex with respect to energy fluctuations in an assessment of energy differences resulting from the nucleotide substitutions will be presented in the context

  3. DNA ELECTROPHORESIS AT SURFACES

    SciTech Connect (OSTI)

    RAFAILOVICH, MIRIAM; SOKOLOV, JONATHAN; GERSAPPE, DILIP

    2003-09-01T23:59:59.000Z

    During this year we performed two major projects: I. We developed a detailed theoretical model which complements our experiments on surface DNA electrophoresis. We found that it was possible to enhance the separation of DNA chains by imposing a chemical nanoscale pattern on the surface. This approach utilized the surface interaction effect of the DNA chains with the substrate and is a refinement to our previous method in which DNA chains were separated on homogeneous flat surfaces. By introducing the nano-patterns on the surface, the conformational changes of DNA chains of different lengths can be amplified, which results in the different friction strengths with the substrate surface. Our results also show that, when compared to the DNA electrophoresis performed on homogeneous flat surfaces, nanopatterned surfaces offer a larger window in choosing different surface interactions to achieve separation. II. In collaboration with a large international manufacturer of skin care products we also embarked on a project involving photo toxicity of titanium dioxide nanoparticles, which are a key ingredient in sunscreen and cosmetic lotions. The results clearly implicated the nanoparticles in catalyzing damage to chromosomal DNA. We then used this knowledge to develop a polymer/anti-oxidant coating which prevented the photocatalytic reaction on DNA while still retaining the UV absorptive properties of the nanoparticles. The standard gel electrophoresis was not sufficient in determining the extent of the DNA damage. The conclusions of this study were based predominantly on analysis obtained with the surface electrophoresis method.

  4. Multiprotein DNA looping

    E-Print Network [OSTI]

    Jose M. G. Vilar; Leonor Saiz

    2006-06-19T23:59:59.000Z

    DNA looping plays a fundamental role in a wide variety of biological processes, providing the backbone for long range interactions on DNA. Here we develop the first model for DNA looping by an arbitrarily large number of proteins and solve it analytically in the case of identical binding. We uncover a switch-like transition between looped and unlooped phases and identify the key parameters that control this transition. Our results establish the basis for the quantitative understanding of fundamental cellular processes like DNA recombination, gene silencing, and telomere maintenance.

  5. Visualizing DNA What is it?

    E-Print Network [OSTI]

    Rose, Michael R.

    Visualizing DNA #12;What is it? Gel electrophoresis is one of the techniques scientists use to look at the DNA they have. This technique separates DNA by size. #12;How does it work? First a gel is prepared. Gels

  6. Faience Technology

    E-Print Network [OSTI]

    Nicholson, Paul

    2009-01-01T23:59:59.000Z

    by Joanne Hodges. Faience Technology, Nicholson, UEE 2009Egyptian materials and technology, ed. Paul T. Nicholson,Nicholson, 2009, Faience Technology. UEE. Full Citation:

  7. Enhancing the DNA Patent Database

    SciTech Connect (OSTI)

    Walters, LeRoy B.

    2008-02-18T23:59:59.000Z

    Final Report on Award No. DE-FG0201ER63171 Principal Investigator: LeRoy B. Walters February 18, 2008 This project successfully completed its goal of surveying and reporting on the DNA patenting and licensing policies at 30 major U.S. academic institutions. The report of survey results was published in the January 2006 issue of Nature Biotechnology under the title “The Licensing of DNA Patents by US Academic Institutions: An Empirical Survey.” Lori Pressman was the lead author on this feature article. A PDF reprint of the article will be submitted to our Program Officer under separate cover. The project team has continued to update the DNA Patent Database on a weekly basis since the conclusion of the project. The database can be accessed at dnapatents.georgetown.edu. This database provides a valuable research tool for academic researchers, policymakers, and citizens. A report entitled Reaping the Benefits of Genomic and Proteomic Research: Intellectual Property Rights, Innovation, and Public Health was published in 2006 by the Committee on Intellectual Property Rights in Genomic and Protein Research and Innovation, Board on Science, Technology, and Economic Policy at the National Academies. The report was edited by Stephen A. Merrill and Anne-Marie Mazza. This report employed and then adapted the methodology developed by our research project and quoted our findings at several points. (The full report can be viewed online at the following URL: http://www.nap.edu/openbook.php?record_id=11487&page=R1). My colleagues and I are grateful for the research support of the ELSI program at the U.S. Department of Energy.

  8. DNA-PK assay

    DOE Patents [OSTI]

    Anderson, Carl W.; Connelly, Margery A.

    2004-10-12T23:59:59.000Z

    The present invention provides a method for detecting DNA-activated protein kinase (DNA-PK) activity in a biological sample. The method includes contacting a biological sample with a detectably-labeled phosphate donor and a synthetic peptide substrate defined by the following features to provide specific recognition and phosphorylation by DNA-PK: (1) a phosphate-accepting amino acid pair which may include serine-glutamine (Ser-Gln) (SQ), threonine-glutamine (Thr-Gln) (TQ), glutamine-serine (Gln-Ser) (QS), or glutamine-threonine (Gln-Thr) (QT); (2) enhancer amino acids which may include glutamic acid or glutamine immediately adjacent at the amino- or carboxyl- side of the amino acid pair and forming an amino acid pair-enhancer unit; (3) a first spacer sequence at the amino terminus of the amino acid pair-enhancer unit; (4) a second spacer sequence at the carboxyl terminus of the amino acid pair-enhancer unit, which spacer sequences may include any combination of amino acids that does not provide a phosphorylation site consensus sequence motif; and, (5) a tag moiety, which may be an amino acid sequence or another chemical entity that permits separating the synthetic peptide from the phosphate donor. A compostion and a kit for the detection of DNA-PK activity are also provided. Methods for detecting DNA, protein phosphatases and substances that alter the activity of DNA-PK are also provided. The present invention also provides a method of monitoring protein kinase and DNA-PK activity in living cells. -A composition and a kit for monitoring protein kinase activity in vitro and a composition and a kit for monitoring DNA-PK activities in living cells are also provided. A method for identifying agents that alter protein kinase activity in vitro and a method for identifying agents that alter DNA-PK activity in living cells are also provided.

  9. DNA chips --Integrated Chemical Circuits for DNADiagnosis and DNA computers

    E-Print Network [OSTI]

    Hagiya, Masami

    DNA chips -- Integrated Chemical Circuits for DNADiagnosis and DNA computers Akira Suyama, Associate Professor Institute of Physics, Graduate School of Arts and Sciences, The University of Tokyo DNA chips are si l i con­ or glass­based smal l surfaces on which many DNA ol i gonuc l eotides are i

  10. Focus: DNA probes

    SciTech Connect (OSTI)

    Not Available

    1986-11-01T23:59:59.000Z

    Progress in the development of DNA probes for the identification and quantitation of specific genetic sequences in biological samples is reviewed. Current research efforts in the development of DNA probes for the diagnosis of a wide variety of bacterial, viral, and other infectious diseases, such as herpes simplex and cytomegalovirus, and inherited genetic diseases such as cystic fibrosis and sickle cell anemia are discussed. Progress in development of DNA probe assays for cancer diagnosis, detection of Salmonella food poisoning, tissue typing (detection of histocompatibility antigens), mutagen screening, and animal diseases, among other applications is included.

  11. -DNA 1217 BK21-IT,

    E-Print Network [OSTI]

    - DNA 1217 BK21-IT, (MEC) (NRL) . . : : : : syshin@bi.snu.ac.kr ihlee@bi.snu.ac.kr btzhang@bi.snu.ac.kr 2004 9 16 2005 10 14 - DNA (DNA Sequence Design using -Multiobjective Evolutionary Algorithm) (Soo-Yong Shin) (In-Hee Lee) (Byoung-Tak Zhang) DNA

  12. Shear Unzipping of DNA

    E-Print Network [OSTI]

    Buddhapriya Chakrabarti; David R. Nelson

    2009-04-09T23:59:59.000Z

    We study theoretically the mechanical failure of a simple model of double stranded DNA under an applied shear. Starting from a more microscopic Hamiltonian that describes a sheared DNA, we arrive at a nonlinear generalization of a ladder model of shear unzipping proposed earlier by deGennes [deGennes P. G. C. R. Acad. Sci., Ser. IV; Phys., Astrophys. 2001, 1505]. Using this model and a combination of analytical and numerical methods, we study the DNA "unzipping" transition when the shearing force exceeds a critical threshold at zero temperature. We also explore the effects of sequence heterogeneity and finite temperature and discuss possible applications to determine the strength of colloidal nanoparticle assemblies functionalized by DNA.

  13. Intermediate DNA at low added salt: DNA bubbles slow the diffusion of short DNA fragments

    E-Print Network [OSTI]

    Tomislav Vuletic; Sanja Dolanski Babic; Ticijana Ban; Joachim Raedler; Francoise Livolant; Silvia Tomic

    2011-01-05T23:59:59.000Z

    We report a study of DNA (150 bp fragments) conformations in very low added salt $DNA concentration range $0.0015\\leq c \\leq 8$~mM (bp). We found an intermediate DNA conformation in the region $0.05 DNA has the diffusion coefficient, $D_p$ reduced below the values for both ssDNA coils and native dsDNA helices of similar polymerization degree $N$. Thus, this DNA population can not be a simple mix of dsDNA and of ssDNA which results from DNA melting. Here, melting occurs due to a reduction in screening concomitant with DNA concentration being reduced, in already very low salt conditions. The intermediate DNA is rationalized through the well known concept of fluctuational openings (DNA bubbles) which we postulate to form in AT-rich portions of the sequence, without the strands coming apart. Within the bubbles, DNA is locally stretched, while the whole molecule remains rod-like due to very low salt environment. Therefore, such intermediate DNA is elongated, in comparison to dsDNA, which accounts for its reduced $D_p$.

  14. Strandwise translocation of a DNA glycosylase on undamaged DNA

    SciTech Connect (OSTI)

    Qi, Yan; Nam, Kwangho; Spong, Marie C.; Banerjee, Anirban; Sung, Rou-Jia; Zhang, Michael; Karplus, Martin; Verdine, Gregory L. (Harvard)

    2012-05-14T23:59:59.000Z

    Base excision repair of genotoxic nucleobase lesions in the genome is critically dependent upon the ability of DNA glycosylases to locate rare sites of damage embedded in a vast excess of undamaged DNA, using only thermal energy to fuel the search process. Considerable interest surrounds the question of how DNA glycosylases translocate efficiently along DNA while maintaining their vigilance for target damaged sites. Here, we report the observation of strandwise translocation of 8-oxoguanine DNA glycosylase, MutM, along undamaged DNA. In these complexes, the protein is observed to translocate by one nucleotide on one strand while remaining untranslocated on the complementary strand. We further report that alterations of single base-pairs or a single amino acid substitution (R112A) can induce strandwise translocation. Molecular dynamics simulations confirm that MutM can translocate along DNA in a strandwise fashion. These observations reveal a previously unobserved mode of movement for a DNA-binding protein along the surface of DNA.

  15. Coarse-graining DNA for simulations of DNA nanotechnology

    E-Print Network [OSTI]

    Doye, Jonathan P K; Louis, Ard A; Romano, Flavio; Sulc, Petr; Matek, Christian; Snodin, Benedict E K; Rovigatti, Lorenzo; Schreck, John S; Harrison, Ryan M; Smith, William P J

    2013-01-01T23:59:59.000Z

    To simulate long time and length scale processes involving DNA it is necessary to use a coarse-grained description. Here we provide an overview of different approaches to such coarse graining, focussing on those at the nucleotide level that allow the self-assembly processes associated with DNA nanotechnology to be studied. OxDNA, our recently-developed coarse-grained DNA model, is particularly suited to this task, and has opened up this field to systematic study by simulations. We illustrate some of the range of DNA nanotechnology systems to which the model is being applied, as well as the insights it can provide into fundamental biophysical properties of DNA.

  16. Hairpin DNA Switch for Ultrasensitive Spectrophotometric Detection...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Hairpin DNA Switch for Ultrasensitive Spectrophotometric Detection of DNA Hybridization Based on Gold Nanoparticles and Enzyme Hairpin DNA Switch for Ultrasensitive...

  17. Noninvasive diagnosis of fetal aneuploidy by shotgun sequencing DNA from maternal blood

    E-Print Network [OSTI]

    Quake, Stephen R.

    % of total DNA in maternal cell-free plasma (13). Recently developed methods for aneuploidy detection focus technology from plasma of pregnant women, obtaining, on average, 5 million sequence tags per patient sample as the 14th week. Direct sequencing also allowed us to study the characteristics of cell-free plasma DNA

  18. Molecular display of synthetic oligonucleotide libraries and their analysis with high throughput DNA sequencing

    E-Print Network [OSTI]

    Larman, Harry Benjamin

    2012-01-01T23:59:59.000Z

    High throughput methods in molecular biology have changed the landscape of biomedical research. In particular, advances in massively parallel DNA sequencing and synthesis technologies are defining our genomes and the ...

  19. DNA polymerase having modified nucleotide binding site for DNA sequencing

    DOE Patents [OSTI]

    Tabor, Stanley (Cambridge, MA); Richardson, Charles (Chestnut Hill, MA)

    1997-01-01T23:59:59.000Z

    Modified gene encoding a modified DNA polymerase wherein the modified polymerase incorporates dideoxynucleotides at least 20-fold better compared to the corresponding deoxynucleotides as compared with the corresponding naturally-occurring DNA polymerase.

  20. DNA polymerase having modified nucleotide binding site for DNA sequencing

    DOE Patents [OSTI]

    Tabor, S.; Richardson, C.

    1997-03-25T23:59:59.000Z

    A modified gene encoding a modified DNA polymerase is disclosed. The modified polymerase incorporates dideoxynucleotides at least 20-fold better compared to the corresponding deoxynucleotides as compared with the corresponding naturally-occurring DNA polymerase. 6 figs.

  1. Searching for DNA Lesions: Structural Evidence for Lower- and Higher-Affinity DNA Binding Conformations of Human Alkyladenine DNA Glycosylase

    E-Print Network [OSTI]

    Drennan, Catherine L.

    To efficiently repair DNA, human alkyladenine DNA glycosylase (AAG) must search the million-fold excess of unmodified DNA bases to find a handful of DNA lesions. Such a search can be facilitated by the ability of glycosylases, ...

  2. Advanced Sequencing Technology - Final Technical Report for period February 1, 1994 to January 31, 1997

    SciTech Connect (OSTI)

    Gesteland, Raymond F.

    1997-01-31T23:59:59.000Z

    OAK-B135 This project is to develop advanced technologies for DNA sequencing and genotyping. The core technologies are automated probing of multiplexed membranes and high throughput electro-spray mass spectrometry.

  3. EA-1849-S1: Phase II Facility - Ormat Tuscarora Geothermal Power Plant in

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742Energy China U.S.ContaminationJuly 2011D APPENDIXKahukuCounty,ColoradoAprilAirTuscarora, NV |

  4. EA-1849: Ormat Nevada Geothermal Projects in Northern NV | Department of

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742Energy China U.S.ContaminationJuly 2011D APPENDIXKahukuCounty,ColoradoAprilAirTuscarora,

  5. ORNI 9, LLC, AND ORMAT NEVADA INC. APPLICATIONS FOR PERMIT TO DRILL

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page onYouTube YouTube Note: Since the.pdfBreaking ofOilNEWResponse to Time-BasedDecember 23,Misc Cases TOREM renewsORNI 9,

  6. Ormat Becomes Sole Owner of the Mammoth Complex in Mammoth Lakes,

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to: navigation, searchOfRoseConcernsCompany OilInformation OrganisationFoundationOpenJump

  7. Ormat's North Brawley plant with 17MW short of its 50MW potential | Open

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to: navigation, searchOfRoseConcernsCompany OilInformationPre-Tax Charge for Impairment

  8. DNA Structural Nanotechnology Duke University

    E-Print Network [OSTI]

    Reif, John H.

    DNA Structural Nanotechnology John Reif Duke University Graduate Students: Harish Chandran&Caltech Tube Lattices #12;Ned Seeman New York University, USA Ned Seeman: Father of DNA Nanotechnology His Initial Ideas & Motivation for DNA Nanotechnology #12;Cube Chen & Seeman, Nature350:631 (1991) Truncated

  9. Sensing DNA - DNA as nanosensor: a perspective towards nanobiotechnology

    E-Print Network [OSTI]

    Ralf Metzler; Tobias Ambjoernsson

    2005-08-20T23:59:59.000Z

    Based on modern single molecule techniques, we devise a number of possible experimental setups to probe local properties of DNA such as the presence of DNA-knots, loops or folds, or to obtain information on the DNA-sequence. Similarly, DNA may be used as a local sensor. Employing single molecule fluorescence methods, we propose to make use of the physics of DNA denaturation nanoregions to find out about the solvent conditions such as ionic strength, presence of binding proteins, etc. By measuring dynamical quantities in particular, rather sensitive nanoprobes may be constructed with contemporary instruments.

  10. Bioinformatics: Organisms from Venus, Technology from Jupiter, Algorithms from

    E-Print Network [OSTI]

    on the development of bioinformatics, including some visionary discussions on technology, algorithms, systems biology and computational biomedicine. Keywords: Bayesian networks, biclustering, bioinformatics, clustering techniques, computational biology, datamining, DNA chips, dynamical systems, genetic networks, Gibbs sampling, graphical

  11. DNA decontamination: DNA-ExitusPlus in comparison with conventional reagents

    E-Print Network [OSTI]

    Cai, Long

    DNA decontamination: DNA-ExitusPlus in comparison with conventional reagents Here we present a completely new DNA decontamination reagent DNA-ExitusPlus. In comparison with conventional products, DNA solutions for effective DNA decontamination. DNA decontamination reagents use three different molecular prin

  12. Tops and Writhing DNA

    E-Print Network [OSTI]

    Joseph Samuel; Supurna Sinha

    2010-11-30T23:59:59.000Z

    The torsional elasticity of semiflexible polymers like DNA is of biological significance. A mathematical treatment of this problem was begun by Fuller using the relation between link, twist and writhe, but progress has been hindered by the non-local nature of the writhe. This stands in the way of an analytic statistical mechanical treatment, which takes into account thermal fluctuations, in computing the partition function. In this paper we use the well known analogy with the dynamics of tops to show that when subjected to stretch and twist, the polymer configurations which dominate the partition function admit a local writhe formulation in the spirit of Fuller and thus provide an underlying justification for the use of Fuller's "local writhe expression" which leads to considerable mathematical simplification in solving theoretical models of DNA and elucidating their predictions. Our result facilitates comparison of the theoretical models with single molecule micromanipulation experiments and computer simulations.

  13. DNA waves and water

    E-Print Network [OSTI]

    L. Montagnier; J. Aissa; E. Del Giudice; C. Lavallee; A. Tedeschi; G. Vitiello

    2010-12-23T23:59:59.000Z

    Some bacterial and viral DNA sequences have been found to induce low frequency electromagnetic waves in high aqueous dilutions. This phenomenon appears to be triggered by the ambient electromagnetic background of very low frequency. We discuss this phenomenon in the framework of quantum field theory. A scheme able to account for the observations is proposed. The reported phenomenon could allow to develop highly sensitive detection systems for chronic bacterial and viral infections.

  14. Department of Engineering Technology Technology Education

    E-Print Network [OSTI]

    Bieber, Michael

    Department of Engineering Technology Technology Education A Teacher Education Program New Jersey Institute of Technology #12;WHAT WILL YOU LEARN? Technology teachers teach problem-based learning utilizing math, science and technology principles. Technological studies involve students: · Designing

  15. Distributed Energy Technology Characterization (Desiccant Technologies...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Characterization (Desiccant Technologies), January 2004 Distributed Energy Technology Characterization (Desiccant Technologies), January 2004 The purpose of this report is to...

  16. Interstrand DNA-DNA Cross-Link Formation Between Adenine Residues and Abasic Sites in Duplex DNA

    E-Print Network [OSTI]

    Gates, Kent. S.

    Interstrand DNA-DNA Cross-Link Formation Between Adenine Residues and Abasic Sites in Duplex DNA of DNA is a common event that generates an abasic (Ap) site (1). Ap sites exist as an equilibrating that can form covalent adducts with nucleophilic sites in DNA. Thus, Ap sites present a potentially

  17. Vehicle Technologies Office: 2014 Electric Drive Technologies...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Electric Drive Technologies Annual Progress Report Vehicle Technologies Office: 2014 Electric Drive Technologies Annual Progress Report The Electric Drive Technologies research and...

  18. Method for assaying clustered DNA damages

    DOE Patents [OSTI]

    Sutherland, Betsy M.

    2004-09-07T23:59:59.000Z

    Disclosed is a method for detecting and quantifying clustered damages in DNA. In this method, a first aliquot of the DNA to be tested for clustered damages with one or more lesion-specific cleaving reagents under conditions appropriate for cleavage of the DNA to produce single-strand nicks in the DNA at sites of damage lesions. The number average molecular length (Ln) of double stranded DNA is then quantitatively determined for the treated DNA. The number average molecular length (Ln) of double stranded DNA is also quantitatively determined for a second, untreated aliquot of the DNA. The frequency of clustered damages (.PHI..sub.c) in the DNA is then calculated.

  19. Technology '90

    SciTech Connect (OSTI)

    Not Available

    1991-01-01T23:59:59.000Z

    The US Department of Energy (DOE) laboratories have a long history of excellence in performing research and development in a number of areas, including the basic sciences, applied-energy technology, and weapons-related technology. Although technology transfer has always been an element of DOE and laboratory activities, it has received increasing emphasis in recent years as US industrial competitiveness has eroded and efforts have increased to better utilize the research and development resources the laboratories provide. This document, Technology '90, is the latest in a series that is intended to communicate some of the many opportunities available for US industry and universities to work with the DOE and its laboratories in the vital activity of improving technology transfer to meet national needs. Technology '90 is divided into three sections: Overview, Technologies, and Laboratories. The Overview section describes the activities and accomplishments of the DOE research and development program offices. The Technologies section provides descriptions of new technologies developed at the DOE laboratories. The Laboratories section presents information on the missions, programs, and facilities of each laboratory, along with a name and telephone number of a technology transfer contact for additional information. Separate papers were prepared for appropriate sections of this report.

  20. DNA attachment to support structures

    DOE Patents [OSTI]

    Balhorn, Rodney L. (Livermore, CA); Barry, Christopher H. (Fresno, CA)

    2002-01-01T23:59:59.000Z

    Microscopic beads or other structures are attached to nucleic acids (DNA) using a terminal transferase. The transferase adds labeled dideoxy nucleotide bases to the ends of linear strands of DNA. The labels, such as the antigens digoxigenin and biotin, bind to the antibody compounds or other appropriate complementary ligands, which are bound to the microscopic beads or other support structures. The method does not require the synthesis of a synthetic oligonucleotide probe. The method can be used to tag or label DNA even when the DNA has an unknown sequence, has blunt ends, or is a very large fragment (e.g., >500 kilobase pairs).

  1. A Model for Structure and Thermodynamics of ssDNA and dsDNA Near...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Structure and Thermodynamics of ssDNA and dsDNA Near a Surface:A Coarse Grained Approach. A Model for Structure and Thermodynamics of ssDNA and dsDNA Near a Surface:A Coarse...

  2. B-DNA Under Stress: Over- and Untwisting of DNA during MolecularDynami...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    B-DNA Under Stress: Over- and Untwisting of DNA during MolecularDynamics Simulations. B-DNA Under Stress: Over- and Untwisting of DNA during MolecularDynamics Simulations....

  3. Controlling DNA Methylation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOEThe Bonneville Power Administration would likeConstitution And BylawsMetal-Organic FrameworksControlling DNA

  4. DNA-DNA interactions Helmut H Strey*t, Rudi Podgornik* ,

    E-Print Network [OSTI]

    Podgornik, Rudolf

    309 DNA-DNA interactions Helmut H Strey*t, Rudi Podgornik* , Parsegian The forces that govern DNA interactions - such as electrostatic interactions, hydration, and fluctuation forces - that treat DNA about the physical forces and energies that involve DNA molecules is to ask whether there is more to DNA

  5. Condensation of circular DNA

    E-Print Network [OSTI]

    E. L. Starostin

    2013-04-05T23:59:59.000Z

    A simple model of a circularly closed dsDNA in a poor solvent is considered as an example of a semi-flexible polymer with self-attraction. To find the ground states, the conformational energy is computed as a sum of the bending and torsional elastic components and the effective self-attraction energy. The model includes a relative orientation or sequence dependence of the effective attraction forces between different pieces of the polymer chain. Two series of conformations are analysed: a multicovered circle (a toroid) and a multifold two-headed racquet. The results are presented as a diagram of state. It is suggested that the stability of particular conformations may be controlled by proper adjustment of the primary structure. Application of the model to other semi-flexible polymers is considered.

  6. Evolutionary theory, web-search technology combine for DNA analysis

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of Science (SC) Environmental Assessments (EA) /Email Announcements12:25Sequedex: bioinformatics

  7. FUEL CELL TECHNOLOGIES PROGRAM Technologies

    E-Print Network [OSTI]

    and fuel cells offer great promise for our energy future. Fuel cell vehicles are not yet commercially, such as a hydrogen fueling station or hydrogen fuel cell vehicle. Technology validation does not certify, and the Federal Government to evaluate hydrogen fuel cell vehicle and infrastructure technologies together in real

  8. Sequence independent amplification of DNA

    DOE Patents [OSTI]

    Bohlander, Stefan K. (Chicago, IL)

    1998-01-01T23:59:59.000Z

    The present invention is a rapid sequence-independent amplification procedure (SIA). Even minute amounts of DNA from various sources can be amplified independent of any sequence requirements of the DNA or any a priori knowledge of any sequence characteristics of the DNA to be amplified. This method allows, for example the sequence independent amplification of microdissected chromosomal material and the reliable construction of high quality fluorescent in situ hybridization (FISH) probes from YACs or from other sources. These probes can be used to localize YACs on metaphase chromosomes but also--with high efficiency--in interphase nuclei.

  9. Normalized cDNA libraries

    DOE Patents [OSTI]

    Soares, M.B.; Efstratiadis, A.

    1997-06-10T23:59:59.000Z

    This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3{prime} noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library. 4 figs.

  10. Normalized cDNA libraries

    DOE Patents [OSTI]

    Soares, Marcelo B. (New York, NY); Efstratiadis, Argiris (Englewood, NJ)

    1997-01-01T23:59:59.000Z

    This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3' noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library.

  11. DNA UPTAKE BY TRANSFORMABLE BACTERIA

    SciTech Connect (OSTI)

    LACKS,S.A.

    1999-09-07T23:59:59.000Z

    The various processes of DNA uptake by cells can be categorized as: viral DNA entry, conjugation, or transformation. Within each category, a variety of mechanisms have been found. However, considerable similarities occur among the different mechanisms of conjugation and, especially, transformation. All of these natural mechanisms of DNA transfer are quite elaborate and involve multiple protein components, as the case may be, of the virus, the donor cell, and the recipient cell. The mechanisms of viral infection and conjugation will be discussed mainly with respect to their relevance to transformation.

  12. Transcriptional Control of DNA-Based Nanomachines

    E-Print Network [OSTI]

    Ludwig-Maximilians-Universität, München

    on responses to stimuli or the cell's environ- ment. So far, nanomachines have been controlled with DNA rather be performed by these devices in well-defined steps. Recently, a DNA machine that binds, carries, and releases the manual addition of a "fuel" strand, consisting of single stranded DNA (ssDNA) that affects

  13. Unnatural nucleotides for DNA sequencing

    E-Print Network [OSTI]

    Jacutin, Swanee E

    1997-01-01T23:59:59.000Z

    Fluorescent nucleotide analogs were prepared and tested to find surrogate structures that are: (i) incorporated by DNA polymerases; (ii) spectroscopically distinct for each fluorescent tag; and (iii) easily deprotected at the 3'-position under mild...

  14. Mammalian DNA Repair. Final Report

    SciTech Connect (OSTI)

    None

    2003-01-24T23:59:59.000Z

    The Gordon Research Conference (GRC) on Mammalian DNA Repair was held at Harbortown Resort, Ventura Beach, CA. Emphasis was placed on current unpublished research and discussion of the future target areas in this field.

  15. Ancient DNA Chronology within Sediment Deposits: Are Paleobiological Reconstructions Possible and Is DNA Leaching a Factor?

    E-Print Network [OSTI]

    Shapiro, Beth

    reported the successful extraction of ancient DNA (aDNA) from both frozen and nonfrozen sediments (even for vertical migration of aDNA across strata. To assess the extent of this problem, we extracted aDNA from (Lydolph et al. 2005). Also uncertain is whether the DNA is extracellular and bound to clay minerals

  16. Meta-DNA: A DNA-Based Approach to Synthetic Harish Chandran1

    E-Print Network [OSTI]

    Reif, John H.

    Meta-DNA: A DNA-Based Approach to Synthetic Biology Harish Chandran1 harish@cs.duke.edu Nikhil taken here is to develop a biochemical system which we call meta-DNA (abbre- viated as mDNA), based entirely on strands of DNA as the only component molecules. Our work leverages prior work

  17. Meta-DNA: Synthetic Biology via DNA Nanostructures and Hybridization Reactions

    E-Print Network [OSTI]

    Reif, John H.

    Meta-DNA: Synthetic Biology via DNA Nanostructures and Hybridization Reactions Harish Chandran for desired functionality. The approach of this paper is to develop a biochemical system which we call meta-DNA (abbreviated as mDNA), based entirely on strands of DNA as the only component molecule. Our work leverages

  18. Impact of DNA Twist Accumulation on Progressive Helical Wrapping of Torsionally Constrained DNA

    E-Print Network [OSTI]

    Wang, Wei Hua

    Impact of DNA Twist Accumulation on Progressive Helical Wrapping of Torsionally Constrained DNA Wei (Received 17 July 2012; published 20 November 2012) DNA wrapping is an important mechanism for chromosomal DNA packaging in cells and viruses. Previous studies of DNA wrapping have been performed mostly

  19. DNA Word Design Strategy for Creating Sets of Non-interacting Oligonucleotides for DNA Microarrays

    E-Print Network [OSTI]

    DNA Word Design Strategy for Creating Sets of Non-interacting Oligonucleotides for DNA Microarrays-interacting DNA oligonucleotides for applications in DNA arrays and biosensors is demonstrated. This strategy mismatches with the complements of all the other members in the set. These "DNA word" sets are denoted as nbm

  20. Technology Application Centers: Facilitating Technology Transfer

    E-Print Network [OSTI]

    Kuhel, G. J.

    's approach to technology deployment seeks to blend an industrial customer's priorities with the utility's marketing and customer service objectives. A&C Enercom sees technology deployment as the sum of an equation: technology deployment equals technology...

  1. DNA polymorphism identity determination using flow cytometry

    DOE Patents [OSTI]

    Nolan, John P. (Santa Fe, NM); White, P. Scott (Los Alamos, NM); Cai, Hong (Los Alamos, NM)

    2001-01-01T23:59:59.000Z

    DNA polymorphism identity determination using flow cytometry. Primers designed to be immobilized on microspheres are allowed to anneal to the DNA strand under investigation, and are extended by either DNA polymerase using fluorescent dideoxynucleotides or ligated by DNA ligase to fluorescent reporter oligonucleotides. The fluorescence of either the dideoxynucleotide or the reporter oligonucleotide attached to the immobilized primer is measured by flow cytometry, thereby identifying the nucleotide polymorphism on the DNA strand.

  2. Cellular responses to environmental DNA damage

    SciTech Connect (OSTI)

    Not Available

    1994-08-01T23:59:59.000Z

    This volume contains the proceedings of the conference entitled Cellular Responses to Environmental DNA Damage held in Banff,Alberta December 1--6, 1991. The conference addresses various aspects of DNA repair in sessions titled DNA repair; Basic Mechanisms; Lesions; Systems; Inducible Responses; Mutagenesis; Human Population Response Heterogeneity; Intragenomic DNA Repair Heterogeneity; DNA Repair Gene Cloning; Aging; Human Genetic Disease; and Carcinogenesis. Individual papers are represented as abstracts of about one page in length.

  3. FEMP/NTDP Technology Focus New Technology

    E-Print Network [OSTI]

    FEMP/NTDP Technology Focus New Technology Demonstration Program Technology Focus FEMPFederal Energy Management Program Trends in Energy Management Technology: BCS Integration Technologies ­ Open Communications into a complete EMCIS. The first article [1] covered enabling technologies for emerging energy management systems

  4. Regulation of DNA damage tolerance : studies of the translesion synthesis DNA ploymerase eta in Saccharomyces cerevisiae

    E-Print Network [OSTI]

    Woodruff, Rachel Van Etten

    2008-01-01T23:59:59.000Z

    All organisms must control the effects of DNA damage to protect the integrity of their genomes. In addition to DNA repair, this requires DNA damage tolerance pathways, which allow the continuation of essential processes ...

  5. DNA Repair Decline During Mouse Spermiogenesis Results in the Accumulation of Heritable DNA Damage

    E-Print Network [OSTI]

    Marchetti, Francesco

    2008-01-01T23:59:59.000Z

    van Buul, D.G. de Rooij, DNA double-strand breaks and gamma-for the transition proteins in DNA strand break repair, FEBSBoissonneault, Stimulation of DNA repair by the spermatidal

  6. DNA Strands Attached Inside Single Conical Nanopores: Ionic Pore Characteristics and Insight into DNA Biophysics

    E-Print Network [OSTI]

    Nguyen, Gael; Howorka, Stefan; Siwy, Zuzanna S.

    2011-01-01T23:59:59.000Z

    Nonexponential kinetics of DNA escape from alpha-hemolysin2010), (iii) the speed of DNA transport (Meller et al. 2001;0AJ, UK G. Nguyen et al. : DNA Strands in Single Nanopores

  7. DNA binding specificity of the p73 DNA-binding domain

    E-Print Network [OSTI]

    Tse, Pui Wah

    2011-01-01T23:59:59.000Z

    interactions of p53 with DNA: when flexibility serves2006). Structural basis of DNA recognition by p53 tetramers.Z. (2010). Diversity in DNA recognition by p53 revealed by

  8. DNA repair decline during mouse spermiogenesis results in the accumulation of heritable DNA damage

    E-Print Network [OSTI]

    Marchetti, Francesco

    2008-01-01T23:59:59.000Z

    van Buul, D.G. de Rooij, DNA double-strand breaks and gamma-for the transition proteins in DNA strand break repair, FEBSBoissonneault, Stimulation of DNA repair by the spermatidal

  9. Quantitative measurement and modeling of the DNA damage signaling network : DNA double-strand breaks

    E-Print Network [OSTI]

    Tentner, Andrea R. (Andrea Ruth)

    2009-01-01T23:59:59.000Z

    DNA double-strand breaks (DSB) are one of the major mediators of chemotherapy-induced cytotoxicity in tumors. Cells that experience DNA damage can initiate a DNA damage-mediated cell-cycle arrest, attempt to repair the ...

  10. Science & Technology Review September 2009

    SciTech Connect (OSTI)

    Bearinger, J P

    2009-07-24T23:59:59.000Z

    This month's issue has the following articles: (1) Remembering the Laboratory's First Director - Commentary by Harold Brown; (2) Herbert F. York (1921-2009): A Life of Firsts, an Ambassador for Peace - The Laboratory's first director, who died on May 19, 2009, used his expertise in science and technology to advance arms control and prevent nuclear war; (3) Searching for Life in Extreme Environments - DNA will help researchers discover new marine species and prepare to search for life on other planets; (4) Energy Goes with the Flow - Lawrence Livermore is one of the few organizations that distills the big picture about energy resources and use into a concise diagram; and (5) The Radiant Side of Sound - An experimental method that converts sound waves into light may lead to new technologies for scientific and industrial applications.

  11. (Environmental technology)

    SciTech Connect (OSTI)

    Boston, H.L.

    1990-10-12T23:59:59.000Z

    The traveler participated in a conference on environmental technology in Paris, sponsored by the US Embassy-Paris, US Environmental Protection Agency (EPA), the French Environmental Ministry, and others. The traveler sat on a panel for environmental aspects of energy technology and made a presentation on the potential contributions of Oak Ridge National Laboratory (ORNL) to a planned French-American Environmental Technologies Institute in Chattanooga, Tennessee, and Evry, France. This institute would provide opportunities for international cooperation on environmental issues and technology transfer related to environmental protection, monitoring, and restoration at US Department of Energy (DOE) facilities. The traveler also attended the Fourth International Conference on Environmental Contamination in Barcelona. Conference topics included environmental chemistry, land disposal of wastes, treatment of toxic wastes, micropollutants, trace organics, artificial radionuclides in the environment, and the use biomonitoring and biosystems for environmental assessment. The traveler presented a paper on The Fate of Radionuclides in Sewage Sludge Applied to Land.'' Those findings corresponded well with results from studies addressing the fate of fallout radionuclides from the Chernobyl nuclear accident. There was an exchange of new information on a number of topics of interest to DOE waste management and environmental restoration needs.

  12. SELECTING INFORMATION TECHNOLOGY SECURITY

    E-Print Network [OSTI]

    April 2004 SELECTING INFORMATION TECHNOLOGY SECURITY PRODUCTS Shirley Radack, Editor Computer Security Division Information Technology Laboratory National Institute of Standards and Technology Information technology security prod ucts are essential to better secure infor mation technology (IT) systems

  13. DNA repair decline during mouse spermiogenesis results in the accumulation of heritable DNA damage

    E-Print Network [OSTI]

    Marchetti, Francesco

    2008-01-01T23:59:59.000Z

    male germ cells handle DNA damage? Toxicol. Appl. Pharmacol.strand breaks and DNA base damage at different cellularrelationship to genetic damage, Mutat. Res. 216 (1989) 221-

  14. DNA Repair Decline During Mouse Spermiogenesis Results in the Accumulation of Heritable DNA Damage

    E-Print Network [OSTI]

    Marchetti, Francesco

    2008-01-01T23:59:59.000Z

    male germ cells handle DNA damage? Toxicol. Appl. Pharmacol.strand breaks and DNA base damage at different cellularrelationship to genetic damage, Mutat. Res. 216 (1989) 221-

  15. DNA repair is the target of novel antibiotics

    E-Print Network [OSTI]

    Gunderson, Carl Wayne

    2007-01-01T23:59:59.000Z

    W. & A. M. Segall, (2006) DNA repair, a novel antibacterialjunction-trapping peptides induce DNA damage and chromosomePeptide inhibitors of DNA cleavage by tyrosine recombinases

  16. DNA Guided Self-Assembly of Nanocrystals for Optoelectronic Devices /

    E-Print Network [OSTI]

    Noh, Hyunwoo

    2013-01-01T23:59:59.000Z

    Lithographically Confined DNA Origami. Nat. Nanotech. 2010,and Orientation of Individual DNA Shapes on LithographicallyB. ; Yan, H. ; Liu, Y. DNA-Origami-Directed Self- Assembly

  17. High-Fidelity DNA Hybridization Using Programmable Molecular DNA Devices

    E-Print Network [OSTI]

    Reif, John H.

    of complementary nucleic acid strands is the most basic of all reactions involving nucleic acids, but has a major specific high-fidelity DNA hybridization reactions for tar- get strands of arbitrary length. Our protocol acid strands is the most basic of all reactions involving nucleic acids and a major component of most

  18. Technology disrupted

    SciTech Connect (OSTI)

    Papatheodorou, Y. [CH2M Hill (United States)

    2007-02-15T23:59:59.000Z

    Three years ago, the author presented a report on power generation technologies which in summary said 'no technology available today has the potential of becoming transformational or disruptive in the next five to ten years'. In 2006 the company completed another strategic view research report covering the electric power, oil, gas and unconventional energy industries and manufacturing industry. This article summarises the strategic view findings and then revisits some of the scenarios presented in 2003. The cost per megawatt-hour of the alternatives is given for plants ordered in 2005 and then in 2025. The issue of greenhouse gas regulation is dealt with through carbon sequestration and carbon allowances or an equivalent carbon tax. Results reveal substantial variability through nuclear power, hydro, wind, geothermal and biomass remain competitive through every scenario. Greenhouse gas scenario analysis shows coal still be viable, albeit less competitive against nuclear and renewable technologies. A carbon tax or allowance at $24 per metric ton has the same effect on IGCC cost as a sequestration mandate. However, the latter would hurt gas plants much more than a tax or allowance. Sequestering CO{sub 2} from a gas plant is almost as costly per megawatt-hour as for coal. 5 refs., 5 figs., 5 tabs.

  19. Venus Technology Plan Venus Technology Plan

    E-Print Network [OSTI]

    Rathbun, Julie A.

    Venus Technology Plan May 2014 #12; ii Venus Technology Plan At the Venus Exploration Survey priorities, and (3) develop a Technology Plan for future Venus missions (after a Technology Forum at VEXAG Meeting 11 in November 2013). Here, we present the 2014 Venus Technology Plan

  20. Fuel Cell Technologies Office Science and Technology Policy Fellowship...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Cell Technologies Office Science and Technology Policy Fellowship Opportunities Available Fuel Cell Technologies Office Science and Technology Policy Fellowship Opportunities...

  1. High-Affinity DNA Base Analogs as Supramolecular, Nanoscale Promoters of Macroscopic Adhesion

    E-Print Network [OSTI]

    Sottos, Nancy R.

    High-Affinity DNA Base Analogs as Supramolecular, Nanoscale Promoters of Macroscopic Adhesion Cyrus and Engineering, § Beckman Institute for Advanced Science and Technology, and Department of Materials Science and manufac- tured coatings and composites. Supramolecular interactions are often implicated in various

  2. Sample-to-Sample Fluctuations in Heterogeneous DNA ZH. S. GEVORKIAN,1,2,3

    E-Print Network [OSTI]

    -sample fluctuations of the free energy in the native state, which can be interpreted via the concept of local free bounded base pairs are opened and do not give random contribution to the free energy of native DNA and Technology VNU-ITP, 6 Quarter, Linh Trung Ward, Thu Duc District, Ho Chi Minh City, Vietnam 6 Center

  3. Micropatterned cell arrays for detecting DNA damage

    E-Print Network [OSTI]

    Mittal, Sukant

    2008-01-01T23:59:59.000Z

    Numerous agents are capable of interacting with DNA and damaging it. Permanent changes in the DNA structure can be both mutagenic and cytotoxic; therefore, methods to measure the susceptibility of cells to mutations are ...

  4. Towards Privacy Preserving of Forensic DNA Databases

    E-Print Network [OSTI]

    Liu, Sanmin

    2012-02-14T23:59:59.000Z

    Protecting privacy of individuals is critical for forensic genetics. In a kinship/identity testing, related DNA profiles between user's query and the DNA database need to be extracted. However, unrelated profiles cannot be revealed to each other...

  5. Amplification of chromosomal DNA in situ

    DOE Patents [OSTI]

    Christian, Allen T. (Tracy, CA); Coleman, Matthew A. (Livermore, CA); Tucker, James D. (Livermore, CA)

    2002-01-01T23:59:59.000Z

    Amplification of chromosomal DNA in situ to increase the amount of DNA associated with a chromosome or chromosome region is described. The amplification of chromosomal DNA in situ provides for the synthesis of Fluorescence in situ Hybridization (FISH) painting probes from single dissected chromosome fragments, the production of cDNA libraries from low copy mRNAs and improved in Comparative Genomic Hybridization (CGH) procedures.

  6. DNA Microarrays An R Tutorial

    E-Print Network [OSTI]

    Qiu, Weigang

    Analysis & R Tutorial #12;DNA Microarrays An R Tutorial R: Graphics and Statistics beyond Excel R: an Open Source statistical package (http://www.r-project.org) RStudio: a Graphic User Interface to R (http(stock) # median > range(stock) # mim and max > sum(stock) # sum > var(stock) # variance > sd(stock) # standard

  7. Chromosome specific repetitive DNA sequences

    DOE Patents [OSTI]

    Moyzis, Robert K. (Los Alamos, NM); Meyne, Julianne (Los Alamos, NM)

    1991-01-01T23:59:59.000Z

    A method is provided for determining specific nucleotide sequences useful in forming a probe which can identify specific chromosomes, preferably through in situ hybridization within the cell itself. In one embodiment, chromosome preferential nucleotide sequences are first determined from a library of recombinant DNA clones having families of repetitive sequences. Library clones are identified with a low homology with a sequence of repetitive DNA families to which the first clones respectively belong and variant sequences are then identified by selecting clones having a pattern of hybridization with genomic DNA dissimilar to the hybridization pattern shown by the respective families. In another embodiment, variant sequences are selected from a sequence of a known repetitive DNA family. The selected variant sequence is classified as chromosome specific, chromosome preferential, or chromosome nonspecific. Sequences which are classified as chromosome preferential are further sequenced and regions are identified having a low homology with other regions of the chromosome preferential sequence or with known sequences of other family me This invention is the result of a contract with the Department of Energy (Contract No. W-7405-ENG-36).

  8. Technology and the Box

    E-Print Network [OSTI]

    Maitland, Padma

    2013-01-01T23:59:59.000Z

    its explorations of technology in partnership with radicalPadma Maitland Technology and the Box The room is thedisciplines. The theme of “Technology and the Box” emerged

  9. Hydrogen Technologies Group

    SciTech Connect (OSTI)

    Not Available

    2008-03-01T23:59:59.000Z

    The Hydrogen Technologies Group at the National Renewable Energy Laboratory advances the Hydrogen Technologies and Systems Center's mission by researching a variety of hydrogen technologies.

  10. Innovation and Transportation's Technologies

    E-Print Network [OSTI]

    Garrison, William L.

    2001-01-01T23:59:59.000Z

    decision making. Innovation and technology lock-in hasStage 1 imagine the innovation and technology developmentof emphasizing innovation and technology development. Pull

  11. Protein-DNA Interactions Determine the Shapes of DNA Toroids Condensed in Virus Capsids

    E-Print Network [OSTI]

    Podgornik, Rudolf

    Protein-DNA Interactions Determine the Shapes of DNA Toroids Condensed in Virus Capsids Ame, University of Ljubljana, Ljubljana, Slovenia ABSTRACT DNA toroids that form inside the bacteriophage capsid glycol to the bathing solution. Spermine-DNA toroids present a convex, faceted section with no or minor

  12. Probe and method for DNA detection

    SciTech Connect (OSTI)

    Yeh, Hsin-Chih; Werner, James Henry; Sharma, Jaswinder Kumar; Martinez, Jennifer Suzanne

    2013-07-02T23:59:59.000Z

    A hybridization probe containing two linear strands of DNA lights up upon hybridization to a target DNA using silver nanoclusters that have been templated onto one of the DNA strands. Hybridization induces proximity between the nanoclusters on one strand and an overhang on the other strand, which results in enhanced fluorescence emission from the nanoclusters.

  13. DNA Sequencing via Electron Tunneling Michael Zwolak

    E-Print Network [OSTI]

    Zwolak, Michael

    DNA Sequencing via Electron Tunneling Michael Zwolak Department of Physics Oregon State University-cost DNA sequencing methods would revolutionize medicine: a person could have his/her full genome sequenced of "personalized medicine" is hampered today by the high cost and slow speed of DNA sequencing methods. We

  14. Antibody specific for a DNA repair protein

    DOE Patents [OSTI]

    Petrini, John H.; Morgan, William Francis; Maser, Richard Scott; Carney, James Patrick

    2006-07-11T23:59:59.000Z

    An isolated and purified DNA molecule encoding a DNA repair protein, p95, is provided, as is isolated and purified p95. Also provided are methods of detecting p95 and DNA encoding p95. The invention further provides p95 knock-out mice.

  15. TECHNOLOGY FORUM

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels DataDepartment of Energy Your Density Isn'tOriginEducationVideoStrategic| Department of Energy 51:Cross-Site66 -Topic Groups TECHNOLOGY

  16. Vehicle Technologies Office: 2009 Advanced Vehicle Technology...

    Broader source: Energy.gov (indexed) [DOE]

    Well-to-Wheels Analysis of Energy Use and Greenhouse Gas Emissions of Plug-In Hybrid Electric Vehicles Vehicle Technologies Office: 2008 Advanced Vehicle Technology Analysis...

  17. Vehicle Technologies Office: 2008 Advanced Vehicle Technology...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Vehicle Technology Analysis and Evaluation Activities and Heavy Vehicle Systems Optimization Program Annual Progress Report Vehicle Technologies Office: 2008 Advanced Vehicle...

  18. DNA and the Genetic Code June 14, 2011

    E-Print Network [OSTI]

    Guevara-Vasquez, Fernando

    DNA and the Genetic Code June 14, 2011 DNA and the Genetic Code #12;Protein synthesis The genetic synthesis requires two steps: transcription and translation. DNA and the Genetic Code #12;DNA DNA development. DNA is comprised of 4 bases: guanine (G), adenine (A), cytosine (C), and thymine (T). The bases

  19. Contributing Data to the Fleet DNA Project (Brochure)

    SciTech Connect (OSTI)

    Not Available

    2014-09-01T23:59:59.000Z

    The Fleet DNA clearinghouse of commercial fleet transportation data helps vehicle manufacturers and developers optimize vehicle designs and helps fleet managers choose advanced technologies for their fleets. This online tool - available at www.nrel.gov/fleetdna - provides data summaries and visualizations similar to real-world 'genetics' for medium- and heavy-duty commercial fleet vehicles operating within a variety of vocations. To contribute your fleet data, please contact Adam Duran of the U.S. Department of Energy's National Renewable Energy Laboratory (NREL) at adam.duran@nrel.gov or 303-275-4586.

  20. Dezincing Technology

    SciTech Connect (OSTI)

    Dudek, F.J.; Daniels, E.J. [Argonne National Lab., IL (United States). Energy Service Div.; Morgan, W.A. [Metal Recovery Technologies, Inc., East Chicago, IN (United States)

    1997-08-01T23:59:59.000Z

    Half of the steel produced in the US is derived from scrap. With zinc-coated prompt scrap increasing fivefold since 1980, steel-makers are feeling the effect of increased contaminant loads on their operations. The greatest concern is the cost of treatment before disposal of waste dusts and water that arise from remelting zinc-coated scrap. An economic process is needed to strip and recover the zinc from scrap to provide a low residual scrap for steel- and iron-making. Metal Recovery Technologies, Inc., with the assistance of Argonne National Laboratory, have been developing a caustic leach dezincing process for upgrading galvanized stamping plant scrap into clean scrap with recovery of the zinc. With further development the technology could also process galvanized scrap from obsolete automobiles. This paper will review: (1) the status of recent pilot plant operations and plans for a commercial demonstration facility with a dezincing capacity of up to 250,000 tons/year, (2) the economics of caustic dezincing, and (3) benefits of decreased cost of environmental compliance, raw material savings, and improved operations with use of dezinced scrap.

  1. General com Technology community

    E-Print Network [OSTI]

    Ferrara, Katherine W.

    Campus IT General com m unity Technology community ITsystem owners Campus Council for Information Technology (CCFIT) · ~30 members · Advisory evaluation and review role · Input from faculty, staff, students formal representation on steering team and subcommittees Technology Support Program · Technology support

  2. CSIR TECHNOLOGY AWARDS -2013

    E-Print Network [OSTI]

    Jayaram, Bhyravabotla

    CSIR TECHNOLOGY AWARDS - 2013 GUIDELINES & PROFORMAE FOR NOMINATIONS Planning and Performance 2013 #12;CSIR TECHNOLOGY AWARDS BRIEF DETAILS ,,CSIR Technology Awards were instituted in 1990 to encourage multi-disciplinary in- house team efforts and external interaction for technology development

  3. Northwest Regional Technology Center

    E-Print Network [OSTI]

    Northwest Regional Technology Center for Homeland Security The Northwest Regional Technology Center and deployment of technologies that are effective homeland security solutions for the region, and accelerate technology transfer to the national user community. Foster a collaborative spirit across agencies

  4. Method for sequencing DNA base pairs

    DOE Patents [OSTI]

    Sessler, Andrew M. (Oakland, CA); Dawson, John (Pacific Palisades, CA)

    1993-01-01T23:59:59.000Z

    The base pairs of a DNA structure are sequenced with the use of a scanning tunneling microscope (STM). The DNA structure is scanned by the STM probe tip, and, as it is being scanned, the DNA structure is separately subjected to a sequence of infrared radiation from four different sources, each source being selected to preferentially excite one of the four different bases in the DNA structure. Each particular base being scanned is subjected to such sequence of infrared radiation from the four different sources as that particular base is being scanned. The DNA structure as a whole is separately imaged for each subjection thereof to radiation from one only of each source.

  5. Microfluidic DNA sample preparation method and device

    DOE Patents [OSTI]

    Krulevitch, Peter A. (Pleasanton, CA); Miles, Robin R. (Danville, CA); Wang, Xiao-Bo (San Diego, CA); Mariella, Raymond P. (Danville, CA); Gascoyne, Peter R. C. (Bellaire, TX); Balch, Joseph W. (Livermore, CA)

    2002-01-01T23:59:59.000Z

    Manipulation of DNA molecules in solution has become an essential aspect of genetic analyses used for biomedical assays, the identification of hazardous bacterial agents, and in decoding the human genome. Currently, most of the steps involved in preparing a DNA sample for analysis are performed manually and are time, labor, and equipment intensive. These steps include extraction of the DNA from spores or cells, separation of the DNA from other particles and molecules in the solution (e.g. dust, smoke, cell/spore debris, and proteins), and separation of the DNA itself into strands of specific lengths. Dielectrophoresis (DEP), a phenomenon whereby polarizable particles move in response to a gradient in electric field, can be used to manipulate and separate DNA in an automated fashion, considerably reducing the time and expense involved in DNA analyses, as well as allowing for the miniaturization of DNA analysis instruments. These applications include direct transport of DNA, trapping of DNA to allow for its separation from other particles or molecules in the solution, and the separation of DNA into strands of varying lengths.

  6. advanced sequencing technology: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    sequencing technology First Page Previous Page 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 Next Page Last Page Topic Index 1 DNA sequencing: Clinical...

  7. Particle sizer and DNA sequencer

    DOE Patents [OSTI]

    Olivares, Jose A.; Stark, Peter C.

    2005-09-13T23:59:59.000Z

    An electrophoretic device separates and detects particles such as DNA fragments, proteins, and the like. The device has a capillary which is coated with a coating with a low refractive index such as Teflon.RTM. AF. A sample of particles is fluorescently labeled and injected into the capillary. The capillary is filled with an electrolyte buffer solution. An electrical field is applied across the capillary causing the particles to migrate from a first end of the capillary to a second end of the capillary. A detector light beam is then scanned along the length of the capillary to detect the location of the separated particles. The device is amenable to a high throughput system by providing additional capillaries. The device can also be used to determine the actual size of the particles and for DNA sequencing.

  8. Channel plate for DNA sequencing

    DOE Patents [OSTI]

    Douthart, R.J.; Crowell, S.L.

    1998-01-13T23:59:59.000Z

    This invention is a channel plate that facilitates data compaction in DNA sequencing. The channel plate has a length, a width and a thickness, and further has a plurality of channels that are parallel. Each channel has a depth partially through the thickness of the channel plate. Additionally an interface edge permits electrical communication across an interface through a buffer to a deposition membrane surface. 15 figs.

  9. NREL is a national laboratory of the U.S. Department of Energy, Office of Energy Efficiency & Renewable Energy, operated by the Alliance for Sustainable Energy, LLC.

    E-Print Network [OSTI]

    of Energy's (DOE) Geothermal Technologies Program (GTP). The authors wish to thank Arlene Anderson and GTP, Geothermal Energy Association; Smadar Lavi, Ormat; Martin Piszczalski, Sextant Research; Stephen Ponder, NVNREL is a national laboratory of the U.S. Department of Energy, Office of Energy Efficiency

  10. CMM Technology

    SciTech Connect (OSTI)

    Ward, Robert C.

    2008-10-20T23:59:59.000Z

    This project addressed coordinate measuring machine (CMM) technology and model-based engineering. CMM data analysis and delivery were enhanced through the addition of several machine types to the inspection summary program. CMM hardware and software improvements were made with the purchases of calibration and setup equipment and new model-based software for the creation of inspection programs. Kansas City Plant (KCP) personnel contributed to and influenced the development of dimensional metrology standards. Model-based engineering capabilities were expanded through the development of software for the tolerance analysis of piece parts and for the creation of model-based CMM inspection programs and inspection plans and through the purchase of off-the-shelf software for the tolerance analysis of mechanical assemblies. An obsolete database application used to track jobs in Precision Measurement was replaced by a web-based application with improved query and reporting capabilities. A potential project to address the transformation of the dimensional metrology enterprise at the Kansas City Plant was identified.

  11. The dynamic interplay between DNA damage and metabolism : the metabolic fate and transport of DNA lesions and novel DNA damage derived from intermediary metabolism

    E-Print Network [OSTI]

    Jumpathong, Watthanachai

    2014-01-01T23:59:59.000Z

    The work presented in this thesis explores two novel and complementary facets of endogenous DNA damage: the development of biomarkers of inflammation based on metabolites of DNA damage products and the formation of DNA ...

  12. Photoelectrochemical synthesis of DNA microarrays

    E-Print Network [OSTI]

    Jacobson, Joseph

    Optical addressing of semiconductor electrodes represents a powerful technology that enables the independent and parallel control of a very large number of electrical phenomena at the solid-electrolyte interface. To date, ...

  13. DNA Bubble Life Time in Denaturation

    E-Print Network [OSTI]

    Zh. S. Gevorkian; Chin-Kun Hu

    2010-10-11T23:59:59.000Z

    We have investigated the denaturation bubble life time for a homogeneous as well as for a heterogeneous DNA within a Poland-Scheraga model. It is shown that at criticality the bubble life time for a homogeneous DNA is finite provided that the loop entropic exponent c>2 and has a scaling dependence on DNA length for c<2. Heterogeneity in the thermodynamical limit makes the bubble life time infinite for any entropic exponent.

  14. Plasma technology directory

    SciTech Connect (OSTI)

    Ward, P.P.; Dybwad, G.L.

    1995-03-01T23:59:59.000Z

    The Plasma Technology Directory has two main goals: (1) promote, coordinate, and share plasma technology experience and equipment within the Department of Energy; and (2) facilitate technology transfer to the commercial sector where appropriate. Personnel are averaged first by Laboratory and next by technology area. The technology areas are accelerators, cleaning and etching deposition, diagnostics, and modeling.

  15. Chemical biology of mutagenesis and DNA repair: cellular responses to DNA alkylation

    E-Print Network [OSTI]

    Shrivastav, Nidhi

    The reaction of DNA-damaging agents with the genome results in a plethora of lesions, commonly referred to as adducts. Adducts may cause DNA to mutate, they may represent the chemical precursors of lethal events and they ...

  16. DNA Directed Assembly Probe for Detecting DNA-Protein Interaction in Microarray Format

    E-Print Network [OSTI]

    Ng, Jin Kiat

    Quantifying DNA-protein interaction using DNA microarrays are gaining increasing attention due to their ability to profile specificity of interactions in a high-throughput manner. This paper describes a new approach that ...

  17. Optical Recognition of Converted DNA Nucleotides for Single-Molecule DNA

    E-Print Network [OSTI]

    Optical Recognition of Converted DNA Nucleotides for Single-Molecule DNA Sequencing Using Nanopore among individual nucleotides (nt). The system must be capable of differentiating among the four bases

  18. DNA binding specificity of the p73 DNA-binding domain

    E-Print Network [OSTI]

    Tse, Pui Wah

    2011-01-01T23:59:59.000Z

    of DNA recognition by p53 tetramers. Mol Cell 22, 741-753.site as a self-assembled tetramer. Structure 18, 246- Chene,structure of a p53 core tetramer bound to DNA. Oncogene 28,

  19. Fujita LaboratoryTokyo Instituteof Technology Tokyo Instituteof Technology

    E-Print Network [OSTI]

    Fujita LaboratoryTokyo Instituteof Technology Tokyo Instituteof Technology Fujita LaboratoryTokyo Institute of Technology Tokyo Institute of Technology 231 #12;Fujita LaboratoryTokyo Instituteof Technology Tokyo Instituteof Technology 2 IT #12;Fujita LaboratoryTokyo Instituteof

  20. DNA: The Strand that Connects Us All

    SciTech Connect (OSTI)

    Kaplan, Matt (University of Arizona Genetics Core) [University of Arizona Genetics Core

    2011-03-29T23:59:59.000Z

    Learn how the methods and discoveries of human population genetics are applied for personal genealogical reconstruction and anthropological testing. Dr. Kaplan starts with a short general review of human genetics and the biology behind this form of DNA testing. He looks at how DNA testing is performed and how samples are processed in the University of Arizona laboratory. He also examines examples of personal genealogical results from Family Tree DNA and personal anthropological results from the Genographic Project. Finally, he describes the newest project in the UA laboratory, the DNA Shoah Project.

  1. SnapShot: DNA Polymerases II Mammals

    E-Print Network [OSTI]

    Foti, James J.

    DNA polymerases ensure the faithful duplication of genetic information inside the nuclease and mitochondria of eukaryotic cells and the nucleoid of prokaryotic cells. These remarkable enzymes synthesize polynucleotide ...

  2. DNA sequencing using fluorescence background electroblotting membrane

    DOE Patents [OSTI]

    Caldwell, Karin D. (Salt Lake City, UT); Chu, Tun-Jen (Salt Lake City, UT); Pitt, William G. (Orem, UT)

    1992-01-01T23:59:59.000Z

    A method for the multiplex sequencing on DNA is disclosed which comprises the electroblotting or specific base terminated DNA fragments, which have been resolved by gel electrophoresis, onto the surface of a neutral non-aromatic polymeric microporous membrane exhibiting low background fluorescence which has been surface modified to contain amino groups. Polypropylene membranes are preferably and the introduction of amino groups is accomplished by subjecting the membrane to radio or microwave frequency plasma discharge in the presence of an aminating agent, preferably ammonia. The membrane, containing physically adsorbed DNA fragments on its surface after the electroblotting, is then treated with crosslinking means such as UV radiation or a glutaraldehyde spray to chemically bind the DNA fragments to the membrane through said smino groups contained on the surface thereof. The DNA fragments chemically bound to the membrane are subjected to hybridization probing with a tagged probe specific to the sequence of the DNA fragments. The tagging may be by either fluorophores or radioisotopes. The tagged probes hybridized to said target DNA fragments are detected and read by laser induced fluorescence detection or autoradiograms. The use of aminated low fluorescent background membranes allows the use of fluorescent detection and reading even when the available amount of DNA to be sequenced is small. The DNA bound to the membrances may be reprobed numerous times.

  3. Nucleotide capacitance calculation for DNA sequencing

    SciTech Connect (OSTI)

    Lu, Jun-Qiang [ORNL; Zhang, Xiaoguang [ORNL

    2008-01-01T23:59:59.000Z

    Using a first-principles linear response theory, the capacitance of the DNA nucleotides, adenine, cytosine, guanine and thymine, are calculated. The difference in the capacitance between the nucleotides is studied with respect to conformational distortion. The result suggests that although an alternate current capacitance measurement of a single-stranded DNA chain threaded through a nano-gap electrodes may not sufficient to be used as a stand alone method for rapid DNA sequencing, the capacitance of the nucleotides should be taken into consideration in any GHz-frequency electric measurements and may also serve as an additional criterion for identifying the DNA sequence.

  4. PAVEMENT TECHNOLOGY UPDATE This Technology Transfer Program

    E-Print Network [OSTI]

    California at Berkeley, University of

    PAVEMENT TECHNOLOGY UPDATE This Technology Transfer Program publication is funded by the Division by the University of California Pavement Research Center. The University of California Pavement Research Center Using innovative research and sound engineering principles to improve pavement structures, materials

  5. DNA-guided nanoparticle assemblies

    DOE Patents [OSTI]

    Gang, Oleg; Nykypanchuk, Dmytro; Maye, Mathew; van der Lelie, Daniel

    2013-07-16T23:59:59.000Z

    In some embodiments, DNA-capped nanoparticles are used to define a degree of crystalline order in assemblies thereof. In some embodiments, thermodynamically reversible and stable body-centered cubic (bcc) structures, with particles occupying <.about.10% of the unit cell, are formed. Designs and pathways amenable to the crystallization of particle assemblies are identified. In some embodiments, a plasmonic crystal is provided. In some aspects, a method for controlling the properties of particle assemblages is provided. In some embodiments a catalyst is formed from nanoparticles linked by nucleic acid sequences and forming an open crystal structure with catalytically active agents attached to the crystal on its surface or in interstices.

  6. DNA

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    algorithms that we have developed to see the inner workings of molecular machines," said Thomas Terwilliger, a senior Los Alamos scientist and Laboratory Fellow. In this case,...

  7. DNA

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOEThe Bonneville Power Administration wouldDECOMPOSITION OF CALCIUM SULFATE: A REVIEWThis rcportJ it cdrives

  8. DNA nanotechnology DOI: 10.1002/smll.200500464

    E-Print Network [OSTI]

    Li, Jiali

    DNA nanotechnology DOI: 10.1002/smll.200500464 Towards Rapid DNA Sequencing: Detecting Single- Stranded DNA with a Solid-State Nanopore Hao Yan* and Bingqian Xu* Keywords: · DNA · sequencing · single for rapid detection of single DNA molecules and their sequences. Two types of nanopores have been used

  9. Sequence specific alkylation of DNA by hairpin pyrroleimidazole polyamide conjugates

    E-Print Network [OSTI]

    Stoltz, Brian M.

    Sequence specific alkylation of DNA by hairpin pyrrole­imidazole polyamide conjugates Nicholas R predetermined sequences in the minor groove of DNA with affinities and specificities comparable to those of DNA for covalent reaction at specific DNA sequences and thereby inhibit DNA­protein interactions. Site

  10. Sequence Recognition of DNA by Protein-Induced Conformational Transitions

    E-Print Network [OSTI]

    Williams, Loren

    Sequence Recognition of DNA by Protein-Induced Conformational Transitions Derrick Watkins1 The binding of proteins to specific sequences of DNA is an important feature of virtually all DNA transactions. Proteins recognize specific DNA sequences using both direct readout (sensing types and positions of DNA

  11. DNA Profiling Using Solid-State Nanopores: Detection of DNA-Binding

    E-Print Network [OSTI]

    Meller, Amit

    a 3.5 nm pore results from threading of a dye-intercalated DNA molecule, as compared to the typical for drug development, necessitating new in vitro methods for rapid and low-cost assessment of the binding molecules, which give the DNA/intercalator complex a bulkier structure than that of native DNA. Furthermore

  12. A DNA Based Implementation of an Evolutionary Search for Good Encodings for DNA Computation

    E-Print Network [OSTI]

    Deaton, Russell J.

    lelism, capacity, and power. This potential, however, is limited by the constraints imposed by the DNA chemistry 1]. Adleman 2] introduced a way to solve combina- torial optimization problems with DNA- mental reaction in DNA based computation is hydro- gen bonding between Watson-Crick complement base pairs

  13. Northwestern University Information Technology

    E-Print Network [OSTI]

    Shull, Kenneth R.

    ... Integrated Technology Classrooms Online Lectures Collaborative Course Management Tools ...in any teaching environment Classroom Laptop Mobile Device www.it.northwestern.edu NUITAcademic&ResearchTechnologiesNorthwestern University Information Technology (NUIT) is committed to supporting faculty research

  14. 2010 DOE EERE Vehicle Technologies Program Merit Review ? Technology...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Technology Integration 2010 DOE EERE Vehicle Technologies Program Merit Review Technology Integration Technology integration merit review results 2010amr08.pdf More...

  15. Vehicle Technologies Office: 2010 Fuel Technologies R&D Annual...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Fuel Technologies R&D Annual Progress Report Vehicle Technologies Office: 2010 Fuel Technologies R&D Annual Progress Report The Fuels Technologies subprogram supports fuels and...

  16. Vehicle Technologies Office: News

    Broader source: Energy.gov [DOE]

    EERE intends to issue, on behalf of its Fuel Cell Technologies Office, a Funding Opportunity Announcement (FOA) entitled "Fuel Cell Technologies Incubator: Innovations in Fuel Cell and Hydrogen...

  17. Storage and IO Technology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Burst Buffer User Defined Images Archive Home R & D Storage and IO Technologies Storage and IO Technologies Burst Buffer NVRAM and Burst Buffer Use Cases In collaboration...

  18. Technology Integration Overview

    Broader source: Energy.gov (indexed) [DOE]

    Technology Integration Overview Dennis A. Smith - Clean Cities Deployment Connie Bezanson - Vehicle Education June 17, 2014 VEHICLE TECHNOLOGIES OFFICE This presentation does not...

  19. Integrated Technology Deployment

    Office of Energy Efficiency and Renewable Energy (EERE)

    Integrated technology deployment is a comprehensive approach to implementing solutions that increase the use of energy efficiency and renewable energy technologies. Federal, state, and local...

  20. Technology Transfer Reports

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Advanced Research Projects Agency-Energy (ARPA-E) Oil & Gas Technology Transfer Initiatives USEFUL LINKS Association of University Technology Managers (AUTM) Federal Laboratory...

  1. Green Purchasing & Green Technology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Purchasing & Technology Goals 6 & 7: Green Purchasing & Green Technology Our goal is to purchase and use environmentally sustainable products whenever possible and to implement...

  2. Geothermal Technologies Program Overview

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Jay Nathwani Acting Program Manager Geothermal Technologies Program Office of Energy Efficiency and Renewable Energy The Geothermal Technologies Program Overview May 18 2010 Energy...

  3. States & Emerging Energy Technologies

    Broader source: Energy.gov (indexed) [DOE]

    operations and maintenance, and occupant impact, so not only trying to quantify building energy or technology energy performance, but also the impacts of that technology on users....

  4. Carbon Fiber Technology Facility

    Broader source: Energy.gov (indexed) [DOE]

    The Carbon Fiber Technology Facility is relevant in proving the scale- up of low-cost carbon fiber precursor materials and advanced manufacturing technologies * Significant...

  5. Fuel & Lubricant Technologies

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    May 15, 2012 Kevin Stork, Team Lead VTP Annual Merit Review VTP Fuel & Lubricant Technologies eere.energy.gov 2 | Vehicle Technologies Program Mission Enable advanced combustion...

  6. Morgantown Energy Technology Center, technology summary

    SciTech Connect (OSTI)

    Not Available

    1994-06-01T23:59:59.000Z

    This document has been prepared by the DOE Environmental Management (EM) Office of Technology Development (OTD) to highlight its research, development, demonstration, testing, and evaluation activities funded through the Morgantown Energy Technology Center (METC). Technologies and processes described have the potential to enhance DOE`s cleanup and waste management efforts, as well as improve US industry`s competitiveness in global environmental markets. METC`s R&D programs are focused on commercialization of technologies that will be carried out in the private sector. META has solicited two PRDAs for EM. The first, in the area of groundwater and soil technologies, resulted in twenty-one contact awards to private sector and university technology developers. The second PRDA solicited novel decontamination and decommissioning technologies and resulted in eighteen contract awards. In addition to the PRDAs, METC solicited the first EM ROA in 1993. The ROA solicited research in a broad range of EM-related topics including in situ remediation, characterization, sensors, and monitoring technologies, efficient separation technologies, mixed waste treatment technologies, and robotics. This document describes these technology development activities.

  7. Technology Deployment List | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    Technologies Technology Deployment Technology Deployment List Technology Deployment List The Federal Energy Management Program's (FEMP) Technology Deployment List features...

  8. Technology transfer | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Technology transfer Technology Development and Commercialization at Argonne Read more about Technology Development and Commercialization at Argonne New Director to lead Technology...

  9. alkyladenine dna glycosylase: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    recognition and repair by 3-methyladenine DNA glycosylase I (TAG) Audrey H Metz1II), Helicobacter pylori 3mA DNA glycosylase (MagIII), yeast methyladenine DNA glycosylase...

  10. Single Stranded DNA Induced Assembly of Gold Nanoparticles

    E-Print Network [OSTI]

    Yang, Jun

    The binding affinity of single stranded DNA (ssDNA) for gold nanoparticle surface is studied in this work. The data indicate that the strength of interaction between ssDNA and Au particle surface is closely related to the ...

  11. DNA topology confers sequence specificity to nonspecific architectural proteins

    E-Print Network [OSTI]

    Swigon, David

    DNA topology confers sequence specificity to nonspecific architectural proteins Juan Weia , Luke of DNA change the disorder found in chain molecules randomly decorated by nonspecific, architectural, counter to expectations, in greater quantities and at particular sites along simulated DNA minicircles

  12. DNA Damage, Repair & Replication Using E. Coli Model Systems

    E-Print Network [OSTI]

    Troll, Christopher

    2013-01-01T23:59:59.000Z

    431. Kunkel, T.A. (2004) DNA replication fidelity. J Biol1996) Generation of an endogenous DNA- methylating agent bypombe Mag1 alkylpurine DNA glycosylase. EMBO Rep, 12, 1286-

  13. Electrokinetic Concentration of DNA Polymers in Nanofluidic Channels

    E-Print Network [OSTI]

    Dekker, Cees

    Electrokinetic Concentration of DNA Polymers in Nanofluidic Channels Derek Stein,, Zeno Deurvorst on this understanding by demonstrating how a nanofluidic device with integrated electrodes can preconcentrate DNA. KEYWORDS Nanofluidic, DNA, electrokinetic, concentration M iniature fluidic devices are having an important

  14. Allostery through protein-induced DNA bubbles

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Traverso, Joseph J.; Manoranjan, Valipuram S.; Bishop, A. R.; Rasmussen, Kim Ų.; Voulgarakis, Nikolaos K.

    2015-03-12T23:59:59.000Z

    Allostery through DNA is increasingly recognized as an important modulator of DNA functions. Here, we show that the coalescence of protein-induced DNA bubbles can mediate allosteric interactions that drive protein aggregation. We propose that such allostery may regulate DNA's flexibility and the assembly of the transcription machinery. Mitochondrial transcription factor A (TFAM), a dual-function protein involved in mitochondrial DNA (mtDNA) packaging and transcription initiation, is an ideal candidate to test such a hypothesis owing to its ability to locally unwind the double helix. Numerical simulations demonstrate that the coalescence of TFAM-induced bubbles can explain experimentally observed TFAM oligomerization. The resultingmore »melted DNA segment, approximately 10 base pairs long, around the joints of the oligomers act as flexible hinges, which explains the efficiency of TFAM in compacting DNA. Since mitochondrial polymerase (mitoRNAP) is involved in melting the transcription bubble, TFAM may use the same allosteric interaction to both recruit mitoRNAP and initiate transcription.« less

  15. Recombinant DNA encoding a desulfurization biocatalyst

    DOE Patents [OSTI]

    Rambosek, John (Seattle, WA); Piddington, Chris S. (Seattle, WA); Kovacevich, Brian R. (Seattle, WA); Young, Kevin D. (Grand Forks, ND); Denome, Sylvia A. (Thompson, ND)

    1994-01-01T23:59:59.000Z

    This invention relates to a recombinant DNA molecule containing a gene or genes which encode a biocatalyst capable of desulfurizing a fossil fuel which contains organic sulfur molecules. For example, the present invention encompasses a recombinant DNA molecule containing a gene or genes of a strain of Rhodococcus rhodochrous.

  16. Recombinant DNA encoding a desulfurization biocatalyst

    DOE Patents [OSTI]

    Rambosek, J.; Piddington, C.S.; Kovacevich, B.R.; Young, K.D.; Denome, S.A.

    1994-10-18T23:59:59.000Z

    This invention relates to a recombinant DNA molecule containing a gene or genes which encode a biocatalyst capable of desulfurizing a fossil fuel which contains organic sulfur molecules. For example, the present invention encompasses a recombinant DNA molecule containing a gene or genes of a strain of Rhodococcus rhodochrous. 13 figs.

  17. Dynamics and control of DNA sequence amplification

    SciTech Connect (OSTI)

    Marimuthu, Karthikeyan [Department of Chemical Engineering and Center for Advanced Process Decision-Making, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213 (United States); Chakrabarti, Raj, E-mail: raj@pmc-group.com, E-mail: rajc@andrew.cmu.edu [Department of Chemical Engineering and Center for Advanced Process Decision-Making, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213 (United States); Division of Fundamental Research, PMC Advanced Technology, Mount Laurel, New Jersey 08054 (United States)

    2014-10-28T23:59:59.000Z

    DNA amplification is the process of replication of a specified DNA sequence in vitro through time-dependent manipulation of its external environment. A theoretical framework for determination of the optimal dynamic operating conditions of DNA amplification reactions, for any specified amplification objective, is presented based on first-principles biophysical modeling and control theory. Amplification of DNA is formulated as a problem in control theory with optimal solutions that can differ considerably from strategies typically used in practice. Using the Polymerase Chain Reaction as an example, sequence-dependent biophysical models for DNA amplification are cast as control systems, wherein the dynamics of the reaction are controlled by a manipulated input variable. Using these control systems, we demonstrate that there exists an optimal temperature cycling strategy for geometric amplification of any DNA sequence and formulate optimal control problems that can be used to derive the optimal temperature profile. Strategies for the optimal synthesis of the DNA amplification control trajectory are proposed. Analogous methods can be used to formulate control problems for more advanced amplification objectives corresponding to the design of new types of DNA amplification reactions.

  18. DNA Motif Representation with Nucleotide Dependency

    E-Print Network [OSTI]

    Chin, Francis Y.L.

    DNA Motif Representation with Nucleotide Dependency Francis Chin1 and Henry Leung1 1 Department, these representations cannot model biological binding sites well because they fail to capture nucleotide interdependence. It has been pointed out by many researchers that the nucleotides of the DNA binding site cannot

  19. Atomic force microscopy of biochemically tagged DNA

    SciTech Connect (OSTI)

    Ogletree, D.F.; Kolbe, W.; Spengler, S.; Salmeron, M. (Lawrence Berkeley Laboratory, CA (United States)); Hansma, H.G.; Bezanilla, M. (Univ. of California, Santa Barbara (United States)); Sano, T.; Smith, C.S.; Cantor, C.R. (Boston Univ., MA (United States))

    1993-05-01T23:59:59.000Z

    Small fragments of DNA of known length were made with the polymerase chain reaction. These fragments had biotin molecules covalently attached at their ends. They were subsequently labeled with a chimeric protein fusion between streptavidin and two immunoglobulin G-binding domains of staphyloccocal protein A. This tetrameric species was expected to bind up to four DNA molecules via their attached biotin moieties. The DNA-protein complex was deposited on mica and imaged with an atomic force microscope. The images revealed the protein chimera at the expected location at the ends of the strands of DNA as well as the expected dimers, trimers, and tetramers of DNA bound to a single protein. 25 refs., 5 figs.

  20. Elastic and Proton Dynamics of the DNA

    E-Print Network [OSTI]

    V. L. Golo

    2008-03-28T23:59:59.000Z

    The subject of this report is the dynamics of elastic system in conjunction with hydrogen bonds of the DNA. We draw attention to the draw-back of the familiar rod model of the DNA, and make a case of constructing models that could accommodate the intrinsic structure of the DNA. In this respect studying the interplay among the elastic system and the protons of the DNA, is of interest, for it could accommodate the inter-strand as well as the tunneling modes of protons. Following this direction, we come to the conclusion that the elastic-proton dynamics may have a bearing on biophysics of the DNA. The phenomenon of point mutations is discussed within this framework.

  1. Flow cytometric detection method for DNA samples

    DOE Patents [OSTI]

    Nasarabadi,Shanavaz (Livermore, CA); Langlois, Richard G. (Livermore, CA); Venkateswaran, Kodumudi S. (Round Rock, TX)

    2011-07-05T23:59:59.000Z

    Disclosed herein are two methods for rapid multiplex analysis to determine the presence and identity of target DNA sequences within a DNA sample. Both methods use reporting DNA sequences, e.g., modified conventional Taqman.RTM. probes, to combine multiplex PCR amplification with microsphere-based hybridization using flow cytometry means of detection. Real-time PCR detection can also be incorporated. The first method uses a cyanine dye, such as, Cy3.TM., as the reporter linked to the 5' end of a reporting DNA sequence. The second method positions a reporter dye, e.g., FAM.TM. on the 3' end of the reporting DNA sequence and a quencher dye, e.g., TAMRA.TM., on the 5' end.

  2. Flow cytometric detection method for DNA samples

    DOE Patents [OSTI]

    Nasarabadi, Shanavaz (Livermore, CA); Langlois, Richard G. (Livermore, CA); Venkateswaran, Kodumudi S. (Livermore, CA)

    2006-08-01T23:59:59.000Z

    Disclosed herein are two methods for rapid multiplex analysis to determine the presence and identity of target DNA sequences within a DNA sample. Both methods use reporting DNA sequences, e.g., modified conventional Taqman.RTM. probes, to combine multiplex PCR amplification with microsphere-based hybridization using flow cytometry means of detection. Real-time PCR detection can also be incorporated. The first method uses a cyanine dye, such as, Cy3.TM., as the reporter linked to the 5' end of a reporting DNA sequence. The second method positions a reporter dye, e.g., FAM, on the 3' end of the reporting DNA sequence and a quencher dye, e.g., TAMRA, on the 5' end.

  3. Quantification of false positive reduction in nucleic acid purification on hemorrhagic fever DNA.

    SciTech Connect (OSTI)

    James, Conrad D.; Pohl, Kenneth Roy; Derzon, Mark Steven; McClain, Jaime; Achyuthan, Komandoor

    2006-11-01T23:59:59.000Z

    Columbia University has developed a sensitive highly multiplexed system for genetic identification of nucleic acid targets. The primary obstacle to implementing this technology is the high rate of false positives due to high levels of unbound reporters that remain within the system after hybridization. The ability to distinguish between free reporters and reporters bound to targets limits the use of this technology. We previously demonstrated a new electrokinetic method for binary separation of kb pair long DNA molecules and oligonucleotides. The purpose of this project 99864 is to take these previous demonstrations and further develop the technique and hardware for field use. Specifically, our objective was to implement separation in a heterogeneous sample (containing target DNA and background oligo), to perform the separation in a flow-based device, and to develop all of the components necessary for field testing a breadboard prototype system.

  4. DNA tethering characterization, enzyme-mediated DNA looping under tension, and nucleosome stability in the force measuring optical tweezers

    E-Print Network [OSTI]

    Gemmen, Gregory John

    2006-01-01T23:59:59.000Z

    M. & Schiessel, H. (2004). DNA spools under tension. Phys.accessibility of nucleosomal DNA. Nat. Struct. Mol. Biol.elasticity of lambda-phage DNA. Science . 265 : 1599–1600.

  5. DNA Damage, Repair & Replication Using E. Coli Model Systems

    E-Print Network [OSTI]

    Troll, Christopher

    2013-01-01T23:59:59.000Z

    from DNA containing dIMP residues by the Escherichia coli,from DNA containing dIMP residues by the Escherichia coli,

  6. Constraint of DNA on Functionalized Graphene Improves Its Biostability...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Constraint of DNA on Functionalized Graphene Improves Its Biostability and Specificity. Constraint of DNA on Functionalized Graphene Improves Its Biostability and Specificity....

  7. ap dna endonuclease: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    specificity Raines, Ronald T. 2 A DNA and restriction enzyme implementation of Turing Ma (Turing machines; Universal Turing machines; recombinant DNA; nonpalindromic...

  8. adenovirus dna sequences: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    description of the given DNA string in terms of a smaller set of distinct domain labels. This yields a minimal domain description of a given DNA sequence, significantly...

  9. andribosomal dna sequences: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    description of the given DNA string in terms of a smaller set of distinct domain labels. This yields a minimal domain description of a given DNA sequence, significantly...

  10. IN VITRO MUTAGENIC AND DNA AND CHROMOSOMAL DAMAGE ACTIVITY BY...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    IN VITRO MUTAGENIC AND DNA AND CHROMOSOMAL DAMAGE ACTIVITY BY SURFACTANT DISPERSION OR SOLVENT EXTRACT OF A REFERENCE DIESEL EXHAUST PARTICULATE MATERIAL IN VITRO MUTAGENIC AND DNA...

  11. Electrochemical Branched-DNA Assay for Polymerase Chain Reaction...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Branched-DNA Assay for Polymerase Chain Reaction-Free Detection and Quantification of Oncogenes in Messenger RNA Electrochemical Branched-DNA Assay for Polymerase Chain...

  12. adapting dna microarray: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Gene Expression Analysis & R Tutorial 12;DNA Microarrays An R Tutorial Functional Genomics Qiu, Weigang 3 Research tools known as DNA microarrays are Biology and Medicine...

  13. archived dna microarrays: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Gene Expression Analysis & R Tutorial 12;DNA Microarrays An R Tutorial Functional Genomics Qiu, Weigang 3 Research tools known as DNA microarrays are Biology and Medicine...

  14. Protein Bridges DNA Base and Nucleotide Excision Repair Pathways

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    a nucleotide from the DNA double helix to its active site to access damaged nucleotides. But unlike AGT and most other known DNA nucleotide-flipping proteins, this...

  15. DNA Replication via Entanglement Swapping

    E-Print Network [OSTI]

    Onur Pusuluk; Cemsinan Deliduman

    2011-03-31T23:59:59.000Z

    Quantum effects are mainly used for the determination of molecular shapes in molecular biology, but quantum information theory may be a more useful tool to understand the physics of life. Organic molecules and quantum circuits/protocols can be considered as hardware and software of living systems that are co-optimized during evolution. We try to model DNA replication in this sense as a multi-body entanglement swapping with a reliable qubit representation of the nucleotides. In our model molecular recognition of a nucleotide triggers an intrabase entanglement corresponding to a superposition state of different tautomer forms. Then, base pairing occurs by swapping intrabase entanglements with interbase entanglements. We examine possible realizations of quantum circuits to be used to obtain intrabase entanglement and swapping protocols to be employed to obtain interbase entanglement. Finally, we discuss possible ways for computational and experimental verification of the model.

  16. Abstract DNA-type systems

    E-Print Network [OSTI]

    Diederik Aerts; Marek Czachor

    2005-12-22T23:59:59.000Z

    An abstract DNA-type system is defined by a set of nonlinear kinetic equations with polynomial nonlinearities that admit soliton solutions associated with helical geometry. The set of equations allows for two different Lax representations: A von Neumann form and a Darboux-covariant Lax pair. We explain why non-Kolmogorovian probability models occurring in soliton kinetics are naturally associated with chemical reactions. The most general known characterization of soliton kinetic equations is given and a class of explicit soliton solutions is discussed. Switching between open and closed states is a generic behaviour of the helices. The effect does not crucially depend on the order of nonlinearity (i.e. types of reactions), a fact that may explain why simplified models possess properties occuring in realistic systems. We explain also why fluctuations based on Darboux transformations will not destroy the dynamics but only switch between a finite number of helical structures.

  17. Department of Information Technology

    E-Print Network [OSTI]

    Flener, Pierre

    Department of Information Technology Human-Computer Interaction http://www.it.uu.se/research/hci #12;InformationTechnology-HCI Department of Information Technology | www.it.uu.se Today's menu Who we and collaboration Teaching KoF 2007, effects? Vision and plans Challenges #12;InformationTechnology

  18. The Technology & Innovation Centre

    E-Print Network [OSTI]

    Mottram, Nigel

    The Technology & Innovation Centre #12;The Technology and Innovation Centre revolutionises the way in Scotland and further afield ­ including power and energy, renewable technologies, photonics and sensors, for industry, the Technology and Innovation Centre has already attracted major partners including Scottish

  19. Predictive Maintenance Technologies

    Broader source: Energy.gov [DOE]

    Several diagnostic technologies and best practices are available to assist Federal agencies with predictive maintenance programs.

  20. Bridging the Technology Innovation

    E-Print Network [OSTI]

    Wirosoetisno, Djoko

    Bridging the Technology Innovation Gap Dr Ceri Williams Director of Medical Technologies Innovation Technologies #12;Distinctive Approach to Translating ResearchWe support innovation to reach TRL 5 enable real and Knowledge Centre #12;What is the Medical Technologies IKC? · All activities centre on research translation

  1. Acidic C-terminal tail of the ssDNA-binding protein of bacteriophage T7 and ssDNA

    E-Print Network [OSTI]

    Richardson, Charles C.

    Acidic C-terminal tail of the ssDNA-binding protein of bacteriophage T7 and ssDNA compete. Prokaryotic ssDNA-binding proteins share a conserved DNA-binding fold and an acidic C-terminal tail. It has been proposed that in the absence of ssDNA, the C-terminal tail contacts the ssDNA-binding cleft

  2. Soil washing technology evaluation

    SciTech Connect (OSTI)

    Suer, A.

    1995-04-01T23:59:59.000Z

    Environmental Restoration Engineering (ERE) continues to review innovative, efficient, and cost effective technologies for SRS soil and/or groundwater remediation. As part of this effort, this technical evaluation provides review and the latest information on the technology for SRS soil remediation. Additional technology evaluation reports will be issued periodically to update these reports. The purpose of this report is to review the soil washing technology and its potential application to SRS soil remediation. To assess whether the Soil Washing technology is a viable option for SRS soil remediation, it is necessary to review the technology/process, technology advantages/limitations, performance, applications, and cost analysis.

  3. Technology in water conservation 

    E-Print Network [OSTI]

    Finch, Dr. Calvin

    2013-01-01T23:59:59.000Z

    2 tx H2O Summer 2013 Column by Dr. Calvin Finch, Water Conservation and Technology Center director WAT E R CONSERVATION & TECHNOLOGY CENTER Securing Our Water Future It is not unusual for individuals to describe water conservation as a... conservation, however, is just as dependent on technological factors. #27;e technology does not have to be complex to be important #20; consider high e#23;ciency toilets and showerheads. #27;ese everyday appliances largely rely on simple technologies...

  4. Technology in water conservation

    E-Print Network [OSTI]

    Finch, Dr. Calvin

    2013-01-01T23:59:59.000Z

    2 tx H2O Summer 2013 Column by Dr. Calvin Finch, Water Conservation and Technology Center director WAT E R CONSERVATION & TECHNOLOGY CENTER Securing Our Water Future It is not unusual for individuals to describe water conservation as a... conservation, however, is just as dependent on technological factors. #27;e technology does not have to be complex to be important #20; consider high e#23;ciency toilets and showerheads. #27;ese everyday appliances largely rely on simple technologies...

  5. Technology Overview Using Case Studies of Alternative Landfill Technologies

    E-Print Network [OSTI]

    Zornberg, Jorge G.

    Technology Overview Using Case Studies of Alternative Landfill Technologies and Associated Regulatory Topics Prepared by Interstate Technology & Regulatory Council Alternative Landfill Technologies of Alternative Landfill Technologies and Associated Regulatory Topics March 2003 Prepared by Interstate

  6. Building Technologies Office Window and Envelope Technologies...

    Energy Savers [EERE]

    by 2000 (10.7 billion in current dollars) Source: American Energy Innovation Council Case Studies on the Government's Role in Energy Technology Innovation "Low-Emissivity...

  7. NATIONAL ENERGY TECHNOLOGY LABORATORY Technology Transfer Novel...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    for "Outstanding Commercialization Success" from the Federal Laboratory Consortium for Technology Transfer. On October 4, 2012, the NETL team who developed this alloy received...

  8. Vehicle Technologies Office: Graduate Automotive Technology Education...

    Broader source: Energy.gov (indexed) [DOE]

    Centers of Excellence to provide future generations of engineers and scientists with knowledge and skills in advanced automotive technologies. By funding curriculum...

  9. Vehicle Technologies Office: Electric Drive Technologies | Department...

    Broader source: Energy.gov (indexed) [DOE]

    Electronics and Electric Motor R&D North American Power Electronics Supply Chain Analysis Benchmarking EV and HEV Technology View all presentations from the 2014 Merit Review....

  10. DNA fragment sizing and sorting by laser-induced fluorescence

    DOE Patents [OSTI]

    Hammond, Mark L. (Angier, NC); Jett, James H. (Los Alamos, NM); Keller, Richard A. (Los Alamos, NM); Marrone, Babetta L. (Los Alamos, NM); Martin, John C. (Los Alamos, NM)

    1996-01-01T23:59:59.000Z

    A method is provided for sizing DNA fragments using high speed detection systems, such as flow cytometry to determine unique characteristics of DNA pieces from a sample. In one characterization the DNA piece is fragmented at preselected sites to produce a plurality of DNA fragments. The DNA piece or the resulting DNA fragments are treated with a dye effective to stain stoichiometrically the DNA piece or the DNA fragments. The fluorescence from the dye in the stained fragments is then examined to generate an output functionally related to the number of nucleotides in each one of the DNA fragments. In one embodiment, the intensity of the fluorescence emissions from each fragment is linearly related to the fragment length. The distribution of DNA fragment sizes forms a characterization of the DNA piece for use in forensic and research applications.

  11. Method of quantitating dsDNA

    DOE Patents [OSTI]

    Stark, Peter C. (Los Alamos, NM); Kuske, Cheryl R. (Los Alamos, NM); Mullen, Kenneth I. (Los Alamos, NM)

    2002-01-01T23:59:59.000Z

    A method for quantitating dsDNA in an aqueous sample solution containing an unknown amount of dsDNA. A first aqueous test solution containing a known amount of a fluorescent dye-dsDNA complex and at least one fluorescence-attenutating contaminant is prepared. The fluorescence intensity of the test solution is measured. The first test solution is diluted by a known amount to provide a second test solution having a known concentration of dsDNA. The fluorescence intensity of the second test solution is measured. Additional diluted test solutions are similarly prepared until a sufficiently dilute test solution having a known amount of dsDNA is prepared that has a fluorescence intensity that is not attenuated upon further dilution. The value of the maximum absorbance of this solution between 200-900 nanometers (nm), referred to herein as the threshold absorbance, is measured. A sample solution having an unknown amount of dsDNA and an absorbance identical to that of the sufficiently dilute test solution at the same chosen wavelength is prepared. Dye is then added to the sample solution to form the fluorescent dye-dsDNA-complex, after which the fluorescence intensity of the sample solution is measured and the quantity of dsDNA in the sample solution is determined. Once the threshold absorbance of a sample solution obtained from a particular environment has been determined, any similarly prepared sample solution taken from a similar environment and having the same value for the threshold absorbance can be quantified for dsDNA by adding a large excess of dye to the sample solution and measuring its fluorescence intensity.

  12. , btzhang}@bi.snu.ac.kr Boosted DNA Computing for Evolutionary Graphical Structure Learning

    E-Print Network [OSTI]

    PLM (Probabilistic Library Model PLM PLM DNA bead seperation PCR (Polymerase Chain Reaction) PLM PLM DNA DNA PLM PLM PLM PLM DNA PLM PLM evolutionary graph structure PLM DNA &$$) Jc`" '& Bc" %6 #12

  13. DNA looping: the consequences and its control

    E-Print Network [OSTI]

    Leonor Saiz; Jose M. G. Vilar

    2006-09-26T23:59:59.000Z

    The formation of DNA loops by proteins and protein complexes is ubiquitous to many fundamental cellular processes, including transcription, recombination, and replication. Here we review recent advances in understanding the properties of DNA looping in its natural context and how they propagate to the cellular behavior through gene regulation. The results of connecting the molecular properties with cellular physiology indicate that looping of DNA in vivo is much more complex and easier than predicted from current models and reveals a wealth of previously unappreciated details.

  14. DNA adsorption at liquid/solid interfaces

    E-Print Network [OSTI]

    Carine Douarche; Robert Cortčs; Steven J. Roser; Jean-Louis Sikorav; Alan Braslau

    2008-09-26T23:59:59.000Z

    DNA adsorption on solid or liquid surfaces is a topic of broad fundamental and applied interest. Here we study by x-ray reflectivity the adsorption of monodisperse double-stranded DNA molecules a positively-charged surface, obtained through chemical grafting of a homogeneous organicmonomolecular layer of N-(2-aminoethyl) dodecanamide on an oxide-free monocrystalline Si(111) wafer. The adsorbed dsDNA is found to embed into the soft monolayer which is deformed in the process. The surface coverage is very high and this adsorbed layer is expected to display 2D nematic ordering.

  15. SHARED TECHNOLOGY TRANSFER PROGRAM

    SciTech Connect (OSTI)

    GRIFFIN, JOHN M. HAUT, RICHARD C.

    2008-03-07T23:59:59.000Z

    The program established a collaborative process with domestic industries for the purpose of sharing Navy-developed technology. Private sector businesses were educated so as to increase their awareness of the vast amount of technologies that are available, with an initial focus on technology applications that are related to the Hydrogen, Fuel Cells and Infrastructure Technologies (Hydrogen) Program of the U.S. Department of Energy. Specifically, the project worked to increase industry awareness of the vast technology resources available to them that have been developed with taxpayer funding. NAVSEA-Carderock and the Houston Advanced Research Center teamed with Nicholls State University to catalog NAVSEA-Carderock unclassified technologies, rated the level of readiness of the technologies and established a web based catalog of the technologies. In particular, the catalog contains technology descriptions, including testing summaries and overviews of related presentations.

  16. CX-001844: Categorical Exclusion Determination | Department of...

    Broader source: Energy.gov (indexed) [DOE]

    Energy, Golden Field Office Ormat Nevada, Inc. (Ormat) would utilize a 3-dimensional seismic survey with shear wave conversion combined with available data to predict the most...

  17. Sequential addition of short DNA oligos in DNA-polymerase-based synthesis reactions

    DOE Patents [OSTI]

    Gardner, Shea N; Mariella, Jr., Raymond P; Christian, Allen T; Young, Jennifer A; Clague, David S

    2013-06-25T23:59:59.000Z

    A method of preselecting a multiplicity of DNA sequence segments that will comprise the DNA molecule of user-defined sequence, separating the DNA sequence segments temporally, and combining the multiplicity of DNA sequence segments with at least one polymerase enzyme wherein the multiplicity of DNA sequence segments join to produce the DNA molecule of user-defined sequence. Sequence segments may be of length n, where n is an odd integer. In one embodiment the length of desired hybridizing overlap is specified by the user and the sequences and the protocol for combining them are guided by computational (bioinformatics) predictions. In one embodiment sequence segments are combined from multiple reading frames to span the same region of a sequence, so that multiple desired hybridizations may occur with different overlap lengths.

  18. Training & Technology Solutions Queens College ~ Office of Converging Technologies ~ Training & Technology Solutions

    E-Print Network [OSTI]

    Johnson Jr.,, Ray

    Training & Technology Solutions Queens College ~ Office of Converging Technologies ~ Training & Technology Solutions 718-997-4875 ~ training@qc.cuny.edu ~ I-Bldg 214 Advisor Center Navigation: Login #12;Training & Technology Solutions Queens College ~ Office of Converging Technologies ~ Training

  19. The unholy trinity: taxonomy, species delimitation and DNA barcoding

    E-Print Network [OSTI]

    DeSalle, Rob

    The unholy trinity: taxonomy, species delimitation and DNA barcoding Rob DeSalle*, Mary G. Egan are clarified and resolved, before the use of DNA as a tool for taxonomy and species delimitation can framework for interweaving classical taxonomy with the goals of `DNA barcoding'. Keywords: DNA barcoding

  20. DNA ARRAY DECODING FROM NONLINEAR MEASUREMENTS BY BELIEF PROPAGATION

    E-Print Network [OSTI]

    DNA ARRAY DECODING FROM NONLINEAR MEASUREMENTS BY BELIEF PROPAGATION Mona A. Sheikh, Shriram Compressed Sensing (CS) and demonstrate its utility in DNA array decoding. In a CS DNA microarray, the array spots identify DNA sequences that are shared between multiple organisms, thereby reduc- ing the number

  1. DNA Oligonucleotide Synthesis in Mesoporous Silicon for Biosensing Applications

    E-Print Network [OSTI]

    Weiss, Sharon

    DNA Oligonucleotide Synthesis in Mesoporous Silicon for Biosensing Applications Jenifer L. Lawrie for improving the sensitivity of label-free optical biosensors based on in-situ synthesis of DNA probes within was utilized as the sensor structure. Synthesis of DNA probe, as well as sensing of target DNA, was verified

  2. DNA Deformation Energy as an Indirect Recognition Mechanism in

    E-Print Network [OSTI]

    Lathrop, Richard H.

    DNA Deformation Energy as an Indirect Recognition Mechanism in Protein-DNA Interactions Kimberly A. Senear Abstract--Proteins that bind to specific locations in genomic DNA control many basic cellular. Deformation energy, which models the energy required to bend DNA from its native shape to its shape when bound

  3. DNA Translocation Governed by Interactions with Solid-State Nanopores

    E-Print Network [OSTI]

    Meller, Amit

    DNA Translocation Governed by Interactions with Solid-State Nanopores Meni Wanunu, Jason Sutin, Ben dynamics of individual DNA molecules through solid-state nanopores in the diameter range 2.7­5 nm. Our with DNA length by two power laws: for short DNA molecules, in the range 150­3500 bp, we find an exponent

  4. DNA Compression Challenge Revisited: a Dynamic Programming Approach

    E-Print Network [OSTI]

    Lonardi, Stefano

    DNA Compression Challenge Revisited: a Dynamic Programming Approach Behshad Behzadi and Fabrice Le Fessant LIX, Ecole Polytechnique, Paris, FRANCE June 21 2005 B. Behzadi, F. Le Fessant (LIX) DNA Compression June 21 2005 1 / 38 #12;Outline 1 DNA Compression Challenge 2 Tools and Methods 3 DNA Compression

  5. DNA Computing: A Research University of Western Ontario

    E-Print Network [OSTI]

    Kari, Lila

    31 DNA Computing: A Research Snapshot Lila Kari University of Western Ontario Kalpana Mahalingam ........................................... 31-6 31.5 DNA Memory ................................................... 31-8 Nested Primer Molecular Memory · Organic DNA Memory · Design of DNA Sequences 31.6 Computation in Living Cells

  6. Deoxyribose oxidation chemistry and endogenous DNA adducts

    E-Print Network [OSTI]

    Zhou, Xinfeng

    2006-01-01T23:59:59.000Z

    Endogenous and exogenous oxidants react with cellular macromolecules to generate a variety of electrophiles that react with DNA produce cytotoxic and mutagenic adducts. One source of such electrophiles is deoxyribose in ...

  7. DNA Assembly Line for Nano-Construction

    ScienceCinema (OSTI)

    Oleg Gang

    2010-01-08T23:59:59.000Z

    Building on the idea of using DNA to link up nanoparticles scientists at Brookhaven National Lab have designed a molecular assembly line for high-precision nano-construction. Nanofabrication is essential for exploiting the unique properties of nanoparticl

  8. Linear Thermodynamics of Rodlike DNA Filtration

    E-Print Network [OSTI]

    Li, Zirui

    Linear thermodynamics transportation theory is employed to study filtration of rodlike DNA molecules. Using the repeated nanoarray consisting of alternate deep and shallow regions, it is demonstrated that the complex ...

  9. Intriguing DNA Editor Has a Structural Trigger

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Intriguing DNA Editor Has a Structural Trigger Print A powerful new tool for genome editing and gene regulation has emerged in the form of a family of enzymes known as Cas9. Cas9...

  10. A model for melting of confined DNA

    E-Print Network [OSTI]

    Werner, E; Ambjörnsson, T; Mehlig, B

    2015-01-01T23:59:59.000Z

    When DNA molecules are heated they denature. This occurs locally so that loops of molten single DNA strands form, connected by intact double-stranded DNA pieces. The properties of this "melting" transition have been intensively investigated. Recently there has been a surge of interest in this question, caused by experiments determining the properties of partially bound DNA confined to nanochannels. But how does such confinement affect the melting transition? To answer this question we introduce, and solve a model predicting how confinement affects the melting transition for a simple model system by first disregarding the effect of self-avoidance. We find that the transition is smoother for narrower channels. By means of Monte-Carlo simulations we then show that a model incorporating self-avoidance shows qualitatively the same behaviour and that the effect of confinement is stronger than in the ideal case.

  11. The Initiation of Bacterial DNA Replication

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    that the ATP-DnaA active site is in a closed configuration, which allows it to bind nucleotides using conserved residues from neighboring AAA+ protomers. The precise geometry of...

  12. RecA acts in trans to allow replication of damaged DNA by DNA polymerase V

    E-Print Network [OSTI]

    Cox, Michael M.

    , Michael M. Cox2 , Roger Woodgate3 & Myron F. Goodman1 The DNA polymerase V (pol V) and RecA proteins

  13. Next generation sequencing (NGS)technologies and applications

    SciTech Connect (OSTI)

    Vuyisich, Momchilo [Los Alamos National Laboratory

    2012-09-11T23:59:59.000Z

    NGS technology overview: (1) NGS library preparation - Nucleic acids extraction, Sample quality control, RNA conversion to cDNA, Addition of sequencing adapters, Quality control of library; (2) Sequencing - Clonal amplification of library fragments, (except PacBio), Sequencing by synthesis, Data output (reads and quality); and (3) Data analysis - Read mapping, Genome assembly, Gene expression, Operon structure, sRNA discovery, and Epigenetic analyses.

  14. Carbon Nanotube DNA Sensor and Sensing Mechanism

    E-Print Network [OSTI]

    Le Roy, Robert J.

    nanotube (SWNT) DNA sensors and the sensing mechanism. The simple and generic protocol for label for direct label-free detection of DNA hybridization in a biocompatible buffer solution. We also carried out is a field effect device, which has a typical on-current of 3-6 µA at 10 mV source- drain bias and an on-off

  15. Regulation of DNA repair by parkin

    SciTech Connect (OSTI)

    Kao, Shyan-Yuan, E-mail: shyan-yuan_kao@meei.harvard.edu [Eaton Peabody Laboratory, Massachusetts Eye and Ear Infirmary, 243 Charles Street, Boston, MA 02114 (United States)] [Eaton Peabody Laboratory, Massachusetts Eye and Ear Infirmary, 243 Charles Street, Boston, MA 02114 (United States)

    2009-05-01T23:59:59.000Z

    Mutation of parkin is one of the most prevalent causes of autosomal recessive Parkinson's disease (PD). Parkin is an E3 ubiquitin ligase that acts on a variety of substrates, resulting in polyubiquitination and degradation by the proteasome or monoubiquitination and regulation of biological activity. However, the cellular functions of parkin that relate to its pathological involvement in PD are not well understood. Here we show that parkin is essential for optimal repair of DNA damage. Parkin-deficient cells exhibit reduced DNA excision repair that can be restored by transfection of wild-type parkin, but not by transfection of a pathological parkin mutant. Parkin also protects against DNA damage-induced cell death, an activity that is largely lost in the pathological mutant. Moreover, parkin interacts with the proliferating cell nuclear antigen (PCNA), a protein that coordinates DNA excision repair. These results suggest that parkin promotes DNA repair and protects against genotoxicity, and implicate DNA damage as a potential pathogenic mechanism in PD.

  16. Utilities Inspection Technologies

    E-Print Network [OSTI]

    Messock, R. K.

    Preventive and predictive maintenance programs are enhanced by using various inspection technologies to detect problems and potential failures before catastrophic failure. This paper discusses successful inspection technologies that have been...

  17. Technology Readiness Assessment Guide

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2011-09-15T23:59:59.000Z

    The Guide assists individuals and teams involved in conducting Technology Readiness Assessments (TRAs) and developing Technology Maturation Plans (TMPs) for the DOE capital asset projects subject to DOE O 413.3B. Cancels DOE G 413.3-4.

  18. UNIVERSITY of STRATHCLYDE TECHNOLOGY &

    E-Print Network [OSTI]

    Mottram, Nigel

    electricity networks and distribution systems, through to using smart grid technologies for more effective of dynamic collaborations delivering productive outcomes. #12;#12;LOW CARBON POWER AND ENERGY FUTURE CITIES Advanced Manufacturing Future Cities Health Technologies Working collaboratively, programmes within

  19. DNA Polymerase-Mediated DNA Synthesis on a TNA Template John C. Chaput, Justin K. Ichida, and Jack W. Szostak*

    E-Print Network [OSTI]

    Heller, Eric

    DNA Polymerase-Mediated DNA Synthesis on a TNA Template John C. Chaput, Justin K. Ichida, and Jack TNA is capable of antiparallel, Watson-Crick base-pairing with complementary DNA, RNA, and TNA oligo- nucleotides.2 This property is remarkable, given that the TNA repeat unit is one atom shorter than that of DNA

  20. 2014 Annual Merit Review, Vehicle Technologies Office - 08 Technology...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    -1 8. Technology Integration The Technology Integration subprogram accelerates the adoption and use of alternative fuel and advanced technology vehicles to help meet national...

  1. DOE Vehicle Technologies Program 2009 Merit Review Report - Technology...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Integration and Education DOE Vehicle Technologies Program 2009 Merit Review Report - Technology Integration and Education Merit review of DOE Vehicle Technologies Program research...

  2. Vehicle Technologies Office: 2013 Fuel and Lubricant Technologies...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Fuel and Lubricant Technologies R&D Annual Progress Report Vehicle Technologies Office: 2013 Fuel and Lubricant Technologies R&D Annual Progress Report This report describes the...

  3. Vehicle Technologies Office: 2008 Oak Ridge Transportation Technology...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Oak Ridge Transportation Technology Program Annual Report Vehicle Technologies Office: 2008 Oak Ridge Transportation Technology Program Annual Report ornlttpreportfy08.pdf More...

  4. Does Doctrine Drive Technology or Does Technology Drive Doctrine?

    E-Print Network [OSTI]

    Blasko, Dennis

    2010-01-01T23:59:59.000Z

    Brief No. 4 September 2010 Does Doctrine Drive Technology orDoes Technology Drive Doctrine? Dennis Blasko Summary Wthat emphasizes strategy over technology and may hold some

  5. Technology Innovations and Experience Curves for Nitrogen Oxides Control Technologies

    E-Print Network [OSTI]

    Yeh, Sonia; Rubin, Edward S.; Taylor, Margaret R.

    2007-01-01T23:59:59.000Z

    including issues of technology and cost un- certainties, areon NO x Control Technologies and Cost Effectiveness forand other factors on technology cost trends (hence, the

  6. Technology Integration Overview

    Broader source: Energy.gov (indexed) [DOE]

    Identify chronic vehicle or infrastructure field problems * Incident investigations (technology failures) * Capture lessons learned and develop best practices Technical & Problem...

  7. States & Emerging Energy Technologies

    Broader source: Energy.gov [DOE]

    This presentation, given through the DOE's Technical Assitance Program (TAP), provides information on States & Emerging Energy Technologies.

  8. Deployment of Emerging Technologies

    Broader source: Energy.gov [DOE]

    Presentation covers the FUPWG Deployment of Emerging Technologies. Presented by Brad Gustafson, Department of Energy, held on November 1, 2006.

  9. Photovoltaic Technology Incubator Awards

    SciTech Connect (OSTI)

    Not Available

    2007-06-01T23:59:59.000Z

    This factsheet gives an overview of the Photovoltaic (PV) Technology Incubator Awards and the Solar America Initiative (SAI).

  10. Web Technology (elective package)

    E-Print Network [OSTI]

    Franssen, Michael

    Web Technology (elective package) Offered by: Department of Mathematics and Computer Science? Computer Science-based approaches and enabling technologies for the web. Course descriptions Human and efficient. Web Technology The web has become the major source of information retrieval and is playing

  11. SPACE TECHNOLOGY Actual Estimate

    E-Print Network [OSTI]

    SPACE TECHNOLOGY TECH-1 Actual Estimate Budget Authority (in $ millions) FY 2011 FY 2012 FY 2013 FY.7 247.0 Exploration Technology Development 144.6 189.9 202.0 215.5 215.7 214.5 216.5 Notional SPACE TECHNOLOGY OVERVIEW .............................. TECH- 2 SBIR AND STTR

  12. Department of Science, Technology, &

    E-Print Network [OSTI]

    Acton, Scott

    Developing Leaders of Innovation Department of Science, Technology, & Society #12;Understanding the relationship between technology and society is crucial to becoming a successful leader in any field. #12;Our Students The University of Virginia Department of Science, Technology, and Society offers a comprehensive

  13. Technology Forecasting Scenario Development

    E-Print Network [OSTI]

    Technology Forecasting and Scenario Development Newsletter No. 2 October 1998 Systems Analysis was initiated on the establishment of a new research programme entitled Technology Forecasting and Scenario and commercial applica- tion of new technology. An international Scientific Advisory Panel has been set up

  14. New Technology Demonstration Program

    E-Print Network [OSTI]

    New Technology Demonstration Program Technical Brief FEMPFederal Energy Management Program Tom for saving energy in refrigerated walk-in coolers, and to evaluate the potential for this technology in Federal facilities. The focus of this study was on a single manufacturer of the technology, Nevada Energy

  15. Technology Advertising Contact Information

    E-Print Network [OSTI]

    Peters, Richard

    Overview #12;Technology Advertising Contact Information Alex Sheath 8596 4063 asheath Overview Our online Technology section is geared towards an IT professional environment, reaching a range of technology enthusiasts from every day gadget consumers to business decision makers where enterprise solutions

  16. Science, technology and innovation

    E-Print Network [OSTI]

    Sussex, University of

    Science, technology and innovation Taught degrees MSc in Innovation and Sustainability for International Development 1 year full time/2 years part time Technological innovation lies at the heart in the academic and political circles related to the impact of science, technology and innovation in the context

  17. University Libraries Technology Plan

    E-Print Network [OSTI]

    Moore, Paul A.

    Libraries Bowling Green State University #12;Table of Contents Introduction ..................................................................19 Page 2 of 19 Technology Plan, 2003-2005 University Libraries Bowling Green State University #12University Libraries Technology Plan 2003-2005 Page 1 of 19 Technology Plan, 2003-2005 University

  18. Microsoft Technology Centers Novosibirsk

    E-Print Network [OSTI]

    Narasayya, Vivek

    -depth knowledge of Microsoft products and technologies ensure that you benefit from development best practices discovery, tailored product and technology drill-downs, and expert presentations. It culminates practices, and risk analysis to chief technology officers, architects, and senior members of your

  19. Microsoft Technology Centers Philadelphia

    E-Print Network [OSTI]

    Narasayya, Vivek

    -depth knowledge of Microsoft products and technologies ensure that you benefit from development best practices discovery, tailored product and technology drill-downs, and expert presentations. It culminates practices, and risk analysis to chief technology officers, architects, and senior members of your

  20. Microsoft Technology Centers Minneapolis

    E-Print Network [OSTI]

    Hunt, Galen

    -depth knowledge of Microsoft products and technologies ensure that you benefit from development best practices discovery, tailored product and technology drill-downs, and expert presentations. It culminates practices, and risk analysis to chief technology officers, architects, and senior members of your

  1. Technology Innovation Program | Partnerships | ORNL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Innovation Program SHARE Technology Innovation Program The Technology Innovation Program (TIP) is a 1-year program designed to accelerate selected technologies to commercial...

  2. Sandia National Laboratories: Vehicle Technologies

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    EfficiencyVehicle Technologies Vehicle Technologies Combustion Research Facility (CRF) Vehicle Technology programs at Sandia share a common goal: reducing dependence on...

  3. Chevron, GE form Technology Alliance

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    form Technology Alliance February 3, 2014 HOUSTON, TX, Feb. 3, 2014-Chevron Energy Technology Company and GE Oil & Gas announced today the creation of the Chevron GE Technology...

  4. Renewable Energy Technologies | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    Technologies Renewable Energy Technologies Renewable Energy Technologies State, local, and tribal governments can harness renewable energy technologies from natural sources-...

  5. Environmental Technology Verification of Mobile Sources Control...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Environmental Technology Verification of Mobile Sources Control Technologies Environmental Technology Verification of Mobile Sources Control Technologies 2005 Diesel Engine...

  6. Technology reviews: Glazing systems

    SciTech Connect (OSTI)

    Schuman, J.; Rubinstein, F.; Papamichael, K.; Beltran, L.; Lee, E.S.; Selkowitz, S.

    1992-09-01T23:59:59.000Z

    We present a representative review of existing, emerging, and future technology options in each of five hardware and systems areas in envelope and lighting technologies: lighting systems, glazing systems, shading systems, daylighting optical systems, and dynamic curtain wall systems. The term technology is used here to describe any design choice for energy efficiency, ranging from individual components to more complex systems to general design strategies. The purpose of this task is to characterize the state of the art in envelope and lighting technologies in order to identify those with promise for advanced integrated systems, with an emphasis on California commercial buildings. For each technology category, the following activities have been attempted to the extent possible: Identify key performance characteristics and criteria for each technology; determine the performance range of available technologies; identify the most promising technologies and promising trends in technology advances; examine market forces and market trends; and develop a continuously growing in-house database to be used throughout the project. A variety of information sources have been used in these technology characterizations, including miscellaneous periodicals, manufacturer catalogs and cut sheets, other research documents, and data from previous computer simulations. We include these different sources in order to best show the type and variety of data available, however publication here does not imply our guarantee of these data. Within each category, several broad classes are identified, and within each class we examine the generic individual technologies that fag into that class.

  7. Technology reviews: Shading systems

    SciTech Connect (OSTI)

    Schuman, J.; Rubinstein, F.; Papamichael, K.; Beltran, L.; Lee, E.S.; Selkowitz, S.

    1992-09-01T23:59:59.000Z

    We present a representative review of existing, emerging, and future technology options in each of five hardware and systems areas in envelope and lighting technologies: lighting systems, glazing systems, shading systems, daylighting optical systems, and dynamic curtain wall systems. The term technology is used here to describe any design choice for energy efficiency, ranging from individual components to more complex systems to general design strategies. The purpose of this task is to characterize the state of the art in envelope and lighting technologies in order to identify those with promise for advanced integrated systems, with an emphasis on California commercial buildings. For each technology category, the following activities have been attempted to the extent possible: Identify key performance characteristics and criteria for each technology. Determine the performance range of available technologies. Identify the most promising technologies and promising trends in technology advances. Examine market forces and market trends. Develop a continuously growing in-house database to be used throughout the project. A variety of information sources have been used in these technology characterizations, including miscellaneous periodicals, manufacturer catalogs and cut sheets, other research documents, and data from previous computer simulations. We include these different sources in order to best show the type and variety of data available, however publication here does not imply our guarantee of these data. Within each category, several broad classes are identified, and within each class we examine the generic individual technologies that fall into that class.

  8. Optical selection and collection of DNA fragments

    DOE Patents [OSTI]

    Roslaniec, Mary C. (Los Alamos, NM); Martin, John C. (Los Alamos, NM); Jett, James H. (Los Alamos, NM); Cram, L. Scott (Los Alamos, NM)

    1998-01-01T23:59:59.000Z

    Optical selection and collection of DNA fragments. The present invention includes the optical selection and collection of large (>.mu.g) quantities of clonable, chromosome-specific DNA from a sample of chromosomes. Chromosome selection is based on selective, irreversible photoinactivation of unwanted chromosomal DNA. Although more general procedures may be envisioned, the invention is demonstrated by processing chromosomes in a conventional flow cytometry apparatus, but where no droplets are generated. All chromosomes in the sample are first stained with at least one fluorescent analytic dye and bonded to a photochemically active species which can render chromosomal DNA unclonable if activated. After passing through analyzing light beam(s), unwanted chromosomes are irradiated using light which is absorbed by the photochemically active species, thereby causing photoinactivation. As desired chromosomes pass this photoinactivation point, the inactivating light source is deflected by an optical modulator; hence, desired chromosomes are not photoinactivated and remain clonable. The selection and photoinactivation processes take place on a microsecond timescale. By eliminating droplet formation, chromosome selection rates 50 times greater than those possible with conventional chromosome sorters may be obtained. Thus, usable quantities of clonable DNA from any source thereof may be collected.

  9. DNA nanotechnology: understanding and optimisation through simulation

    E-Print Network [OSTI]

    Thomas E. Ouldridge

    2014-11-07T23:59:59.000Z

    DNA nanotechnology promises to provide controllable self-assembly on the nanoscale, allowing for the design of static structures, dynamic machines and computational architectures. In this article I review the state-of-the art of DNA nanotechnology, highlighting the need for a more detailed understanding of the key processes, both in terms of theoretical modelling and experimental characterisation. I then consider coarse-grained models of DNA, mesoscale descriptions that have the potential to provide great insight into the operation of DNA nanotechnology if they are well designed. In particular, I discuss a number of nanotechnological systems that have been studied with oxDNA, a recently developed coarse-grained model, highlighting the subtle interplay of kinetic, thermodynamic and mechanical factors that can determine behaviour. Finally, new results highlighting the importance of mechanical tension in the operation of a two-footed walker are presented, demonstrating that recovery from an unintended `overstepped' configuration can be accelerated by three to four orders of magnitude by application of a moderate tension to the walker's track. More generally, the walker illustrates the possibility of biasing strand-displacement processes to affect the overall rate.

  10. Strategic Technology JET PROPULSION LABORATORY

    E-Print Network [OSTI]

    Waliser, Duane E.

    Strategic Technology Directions JET PROPULSION LABORATORY National Aeronautics and Space Administration 2 0 0 9 #12;© 2009 California Institute of Technology. Government sponsorship acknowledged. #12;Strategic Technology Directions 2009 offers a distillation of technologies, their links to space missions

  11. IIT SCHOOL OF APPLIED TECHNOLOGY

    E-Print Network [OSTI]

    Heller, Barbara

    INDUSTRIAL TECHNOLOGY AND MANAGEMENT IIT SCHOOL OF APPLIED TECHNOLOGY PREPARING SKILLED INDIVIDUALS, INDUSTRIAL FACILITIES, SUPPLY CHAIN MANAGEMENT, SUSTAINABILITY AND MANUFACTURING TECHNOLOGY. #12;BE ONE to assess, implement, and utilize current technologies, and to learn how to manage industrial operations

  12. Solar Energy Resources and Technologies

    Broader source: Energy.gov [DOE]

    Solar energy provides electricity, heating, and cooling for Federal facilities through four primary technology types. The four technologies are broken into two categories; technologies for electricity production and thermal energy technologies.

  13. Technology transfer 1994

    SciTech Connect (OSTI)

    Not Available

    1994-01-01T23:59:59.000Z

    This document, Technology Transfer 94, is intended to communicate that there are many opportunities available to US industry and academic institutions to work with DOE and its laboratories and facilities in the vital activity of improving technology transfer to meet national needs. It has seven major sections: Introduction, Technology Transfer Activities, Access to Laboratories and Facilities, Laboratories and Facilities, DOE Office, Technologies, and an Index. Technology Transfer Activities highlights DOE`s recent developments in technology transfer and describes plans for the future. Access to Laboratories and Facilities describes the many avenues for cooperative interaction between DOE laboratories or facilities and industry, academia, and other government agencies. Laboratories and Facilities profiles the DOE laboratories and facilities involved in technology transfer and presents information on their missions, programs, expertise, facilities, and equipment, along with data on whom to contact for additional information on technology transfer. DOE Offices summarizes the major research and development programs within DOE. It also contains information on how to access DOE scientific and technical information. Technologies provides descriptions of some of the new technologies developed at DOE laboratories and facilities.

  14. Structural Studies of E. coli Topoisomerase III-DNA Complexes Reveal a Novel Type IA Topoisomerase-DNA Conformational Intermediate

    SciTech Connect (OSTI)

    Changela, Anita; DiGate, Russell J.; Mondragon, Alfonso (NWU); (Phil. Col. Pharmacy)

    2010-03-05T23:59:59.000Z

    Escherichia coli DNA topoisomerase III belongs to the type IA family of DNA topoisomerases, which transiently cleave single-stranded DNA (ssDNA) via a 5{prime} phosphotyrosine intermediate. We have solved crystal structures of wild-type E. coli topoisomerase III bound to an eight-base ssDNA molecule in three different pH environments. The structures reveal the enzyme in three distinct conformational states while bound to DNA. One conformation resembles the one observed previously with a DNA-bound, catalytically inactive mutant of topoisomerase III where DNA binding realigns catalytic residues to form a functional active site. Another conformation represents a novel intermediate in which DNA is bound along the ssDNA-binding groove but does not enter the active site, which remains in a catalytically inactive, closed state. A third conformation shows an intermediate state where the enzyme is still in a closed state, but the ssDNA is starting to invade the active site. For the first time, the active site region in the presence of both the catalytic tyrosine and ssDNA substrate is revealed for a type IA DNA topoisomerase, although there is no evidence of ssDNA cleavage. Comparative analysis of the various conformational states suggests a sequence of domain movements undertaken by the enzyme upon substrate binding.

  15. Conditions for positioning of nucleosomes on DNA

    E-Print Network [OSTI]

    Michael Sheinman; Ho-Ryun Chung

    2015-04-29T23:59:59.000Z

    Positioning of nucleosomes along eukaryotic genomes plays an important role in their organization and regulation. There are many different factors affecting the location of nucleosomes. Some can be viewed as preferential binding of a single nucleosome to different locations along the DNA and some as interactions between neighboring nucleosomes. In this study we analyzed how well nucleosomes are positioned along the DNA as a function of strength of the preferential binding, correlation length of the binding energy landscape, interactions between neighboring nucleosomes and others relevant system properties. We analyze different scenarios: designed energy landscapes and generically disordered ones and derive conditions for good positioning. Using analytic and numerical approaches we find that, even if the binding preferences are very weak, synergistic interplay between the interactions and the binding preferences is essential for a good positioning of nucleosomes, especially on correlated energy landscapes. Analyzing empirical energy landscape, we discuss relevance of our theoretical results to positioning of nucleosomes on DNA \\emph{in vivo.}

  16. Geothermal innovative technologies catalog

    SciTech Connect (OSTI)

    Kenkeremath, D. (ed.)

    1988-09-01T23:59:59.000Z

    The technology items in this report were selected on the basis of technological readiness and applicability to current technology transfer thrusts. The items include technologies that are considered to be within 2 to 3 years of being transferred. While the catalog does not profess to be entirely complete, it does represent an initial attempt at archiving innovative geothermal technologies with ample room for additions as they occur. The catalog itself is divided into five major functional areas: Exploration; Drilling, Well Completion, and Reservoir Production; Materials and Brine Chemistry; Direct Use; and Economics. Within these major divisions are sub-categories identifying specific types of technological advances: Hardware; Software; Data Base; Process/Procedure; Test Facility; and Handbook.

  17. Sequential addition of short DNA oligos in DNA-polymerase-based synthesis reactions

    DOE Patents [OSTI]

    Gardner, Shea N. (San Leandro, CA); Mariella, Jr., Raymond P. (Danville, CA); Christian, Allen T. (Tracy, CA); Young, Jennifer A. (Berkeley, CA); Clague, David S. (Livermore, CA)

    2011-01-18T23:59:59.000Z

    A method of fabricating a DNA molecule of user-defined sequence. The method comprises the steps of preselecting a multiplicity of DNA sequence segments that will comprise the DNA molecule of user-defined sequence, separating the DNA sequence segments temporally, and combining the multiplicity of DNA sequence segments with at least one polymerase enzyme wherein the multiplicity of DNA sequence segments join to produce the DNA molecule of user-defined sequence. Sequence segments may be of length n, where n is an even or odd integer. In one embodiment the length of desired hybridizing overlap is specified by the user and the sequences and the protocol for combining them are guided by computational (bioinformatics) predictions. In one embodiment sequence segments are combined from multiple reading frames to span the same region of a sequence, so that multiple desired hybridizations may occur with different overlap lengths. In one embodiment starting sequence fragments are of different lengths, n, n+1, n+2, etc.

  18. Dynamic cluster-scaling in DNA

    E-Print Network [OSTI]

    A. Bershadskii

    2010-08-07T23:59:59.000Z

    It is shown that the nucleotide sequences in DNA molecules have cluster-scaling properties (discovered for the first time in turbulent processes: Sreenivasan and Bershadskii, 2006, J. Stat. Phys., 125, 1141-1153.). These properties are relevant to both types of nucleotide pair-bases interactions: hydrogen bonds and stacking interactions. It is shown that taking into account the cluster-scaling properties can help to improve heterogeneous models of the DNA dynamics. Two human genes: BRCA2 and NRXN1, have been considered as examples.

  19. Ubiquitylation, neddylation and the DNA damage response

    E-Print Network [OSTI]

    Brown, Jessica S.; Jackson, Stephen P.

    2015-04-01T23:59:59.000Z

    major families: RING (really interesting new gene), HECT (homology to E6AP car- boxyl-terminus) and RBR (ring between ring) [102,103]. The transcription, respectively [88] trans-lesion synthesis (TLS) TLS is a DNA damage bypass mechanism tha It employs... depends on RNF4 binding to SUMO2/3 polymeric chains and subsequent RNF4 dimerization [183]. In addition to its role in promoting the turnover of proteins, RNF4 might also be important for the formation of hybrid SUMO/ubiquitin chains at DNA damage sites...

  20. Digital Sensor Technology

    SciTech Connect (OSTI)

    Ted Quinn; Jerry Mauck; Richard Bockhorst; Ken Thomas

    2013-07-01T23:59:59.000Z

    The nuclear industry has been slow to incorporate digital sensor technology into nuclear plant designs due to concerns with digital qualification issues. However, the benefits of digital sensor technology for nuclear plant instrumentation are substantial in terms of accuracy, reliability, availability, and maintainability. This report demonstrates these benefits in direct comparisons of digital and analog sensor applications. It also addresses the qualification issues that must be addressed in the application of digital sensor technology.

  1. Technology Innovation Program 2010ANNUAL REPORT

    E-Print Network [OSTI]

    Technology Innovation Program 2010ANNUAL REPORT 2010ANNUAL REPORT Technology Innovation ProgramTechnology Innovation ProgramTechnology Innovation ProgramTechnology Innovation ProgramTechnology Innovation ProgramTechnology Innovation ProgramTechnology Innovation ProgramTechnology Innovation ProgramTechnology Innovation ProgramTechnology

  2. Additive Manufacturing Technology Assessment

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    market by industrial companies, 58 including the Electro Optical Systems (EOS) in Germany, Arcam in Sweden, MCP Tooling Technologies in 59 the UK, and Stratasys, 3D Systems,...

  3. States & Emerging Energy Technologies

    Broader source: Energy.gov (indexed) [DOE]

    States & Emerging Energy Technologies August 15, 2013 DOE's State and Local Technical Assistance Program 2 DOE's Technical Assistance Program * Strategic Energy Planning * Program...

  4. 2015 Technology Innovation Projects

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    for RTU and Lighting Retrofits Energy Efficiency TIP 140: Energy Efficiency Emerging Technology Assessment and Demonstration Projects TIP 261: Determining and Improving the...

  5. Geothermal Resources and Technologies

    Broader source: Energy.gov [DOE]

    This page provides a brief overview of geothermal energy resources and technologies supplemented by specific information to apply geothermal systems within the Federal sector.

  6. Lighting Technology Panel

    Broader source: Energy.gov [DOE]

    Presentation covers the Lighting Technology Panel for the Federal Utility Partnership Working Group (FUPWG) meeting, held on November 18-19, 2009. 

  7. Climate Vision: Technology Pathways

    Office of Scientific and Technical Information (OSTI)

    Cement Chemical Manufacturing Electric Power Forest Products Iron and Steel Mining Oil and Gas Technology Pathways The DOE's Industries of the Future process helps...

  8. Technology Integration Overview

    Broader source: Energy.gov (indexed) [DOE]

    -Technology Integration Overview - Dennis A. Smith Connie Bezanson U. S. Department of Energy Headquarters Office - Washington, D.C. May 2013 Project ID: TI000 2013 Department of...

  9. Flexible Assembly Solar Technology

    Broader source: Energy.gov (indexed) [DOE]

    Energy, Inc. All rights reserved. 3 About BrightSource Energy We develop and build solar thermal projects using our own central tower technology Headquartered in...

  10. Science, Technology & Engineering

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    to lead LANL Science, Technology & Engineering directorate August 17, 2012 LOS ALAMOS, NEW MEXICO, August 17, 2012-Los Alamos National Laboratory Director Charles McMillan...

  11. Genome Science/Technologies

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    McDonald Bioscience Communications Email State-of-the art technology and extensive genomics expertise Protein research Read caption + Los Alamos National Laboratory graduate...

  12. Solar Energy Technologies Office

    Broader source: Energy.gov [DOE]

    In 2011, the Energy Department's Solar Energy Technologies Office (SETO) became the SunShot Initiative, a collaborative national effort that aggressively drives innovation to make solar energy...

  13. Director, Geothermal Technologies Office

    Broader source: Energy.gov [DOE]

    The mission of the Geothermal Technologies Office (GTO) is to accelerate the development and deployment of clean, domestic geothermal resources that will promote a stronger, more productive economy...

  14. Flexible Assembly Solar Technology

    Broader source: Energy.gov (indexed) [DOE]

    field and secured on steel pylons. PROJECT DESCRIPTION The research team is applying automation processes to the design of a Flexible Assembly Solar Technology (FAST). FAST is an...

  15. Carbon Fiber Technology Facility

    Broader source: Energy.gov (indexed) [DOE]

    conventional and alternative precursors to carbon fiber Advance high-volume composite design and manufacturing capabilities Transition technology to industry partners...

  16. Upgrading through Preprocessing Technologies

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Upgrading through Preprocessing Technologies The challenges of efficiently converting raw biomass into usable, affordable, customized bioenergy feedstdocks are many. The bioenergy...

  17. Arc Position Sensing Technology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    arc remelting (VAR) furnaces for industries that use specialty metals such as nickel, titanium, and zirconium. The technology could be used to help produce materials with stronger...

  18. Mobile Technology Management

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2013-11-21T23:59:59.000Z

    The directive will ensure that federal organizations and employees within the Department can use mobile technology to support mission requirements in a safe and secure manner.

  19. Advanced Propulsion Technology Strategy

    Broader source: Energy.gov (indexed) [DOE]

    Alternative Sources) Hydrogen Time ADVANCED PROPULSION TECHNOLOGY STRATEGY DOWNSIZED TURBO GAS ENGINE CHEVROLET CRUZE 1.4L TURBO ECOTEC Downsized SIDI Turbo Boosting HCCI -...

  20. Technology Demonstration Partnership Policy

    Broader source: Energy.gov [DOE]

    This City Council memorandum establishes a framework for engaging in and evaluating demonstration partnerships with the goal of developing, testing, and demonstrating emerging technologies, product, and service innovations.

  1. Navy Technology Evaluation Update

    Broader source: Energy.gov [DOE]

    Presentation covers the Navy Technology Evaluation update at the Federal Utility Partnership Working Group (FUPWG) meeting, held on November 18-19, 2009.

  2. HVAC Maintenance and Technologies

    Broader source: Energy.gov [DOE]

    Presentation covers the HVAC maintenance and technologies, and is given at the Spring 2010 Federal Utility Partnership Working Group (FUPWG) meeting in Providence, Rhode Island.

  3. Photovoltaic Resources and Technologies

    Broader source: Energy.gov [DOE]

    This page provides a brief overview of photovoltaic (PV) technologies supplemented by specific information to apply PV within the Federal sector.

  4. ENERGY EFFICIENCY TECHNOLOGY ROADMAP

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    to involve more explicit alignment with BPA's newest demand-side roadmap resource, the Demand Response Technology Roadmap. 1 Roadmap chapters have been arranged in stand-alone...

  5. Inhibition of DNA Supercoiling-dependent Transcriptional Activation by a Distant B-DNA to Z-DNA Transition*

    E-Print Network [OSTI]

    Benham, Craig J.

    that a supercoiling-dependent, DNA structural transmission mechanism of this type is responsible for the integration over other alterna- tives, primarily because the change from right-handed helix to left-handed helix). In this way one can alter the destabilization characteristics of other local regions without changing

  6. Training & Technology Solutions Queens College ~ Office of Converging Technologies ~ Training & Technology Solutions

    E-Print Network [OSTI]

    Johnson Jr.,, Ray

    Training & Technology Solutions Queens College ~ Office of Converging Technologies ~ Training & Technology Solutions 718-997-4875 ~ training@qc.cuny.edu ~ I-Bldg 214 CUNYfirst Faculty Center Navigation;Training & Technology Solutions Queens College ~ Office of Converging Technologies ~ Training & Technology

  7. Studies of DNA dynamics in slit-like nanochannel confinement

    E-Print Network [OSTI]

    Balducci, Anthony (Anthony G.)

    2008-01-01T23:59:59.000Z

    The ability to visually observe single DNA molecules has greatly improved our understanding of polymer physics, from gel electrophoresis to the theology of dilute (and even concentrated) polymer solutions. The use of DNA ...

  8. DNA hybridization : fundamental studies and applications in directed assembly

    E-Print Network [OSTI]

    Bajaj, Manish G. (Manish Gopal)

    2005-01-01T23:59:59.000Z

    Programmed self-assembly using non-covalent DNA-DNA interactions is a promising technique for the creation of next-generation functional devices for electronic, optical, and magnetic applications. This thesis develops the ...

  9. Genome scanning : an AFM-based DNA sequencing technique

    E-Print Network [OSTI]

    Elmouelhi, Ahmed (Ahmed M.), 1979-

    2003-01-01T23:59:59.000Z

    Genome Scanning is a powerful new technique for DNA sequencing. The method presented in this thesis uses an atomic force microscope with a functionalized cantilever tip to sequence single stranded DNA immobilized to a mica ...

  10. Protein-DNA interaction, random walks and polymer statistics

    E-Print Network [OSTI]

    Slutsky, Michael

    2005-01-01T23:59:59.000Z

    In Part I of the thesis, a general physical framework describing the kinetics of protein- DNA interaction is developed. Recognition and binding of specific sites on DNA by proteins is central for many cellular functions ...

  11. Role of DNA repair protein ERCC1 in skin cancer 

    E-Print Network [OSTI]

    Song, Liang

    2009-01-01T23:59:59.000Z

    Nucleotide excision repair (NER) is one of the major repair systems for removal of DNA lesions. The NER pathway has evolved mainly to repair UV-induced DNA damage and is also active against a broad range of endogenously ...

  12. Defining functional DNA elements in the human genome

    E-Print Network [OSTI]

    Kellis, Manolis

    With the completion of the human genome sequence, attention turned to identifying and annotating its functional DNA elements. As a complement to genetic and comparative genomics approaches, the Encyclopedia of DNA Elements ...

  13. Lectin cDNA and transgenic plants derived therefrom

    DOE Patents [OSTI]

    Raikhel, Natasha V. (Okemos, MI)

    2000-10-03T23:59:59.000Z

    Transgenic plants containing cDNA encoding Gramineae lectin are described. The plants preferably contain cDNA coding for barley lectin and store the lectin in the leaves. The transgenic plants, particularly the leaves exhibit insecticidal and fungicidal properties.

  14. A model for sample stacking in microcapillary DNA electrophoresis

    E-Print Network [OSTI]

    Srivastava, Alok Kumar, 1967-

    2002-01-01T23:59:59.000Z

    Sanger's method of chain termination is the method of choice in DNA sequencing, where electrophoresis is used to separate the different sized DNA. In the past decade, microfabricated capillary devices have been developed ...

  15. Local alignment of generalized k-base encoded DNA sequence

    E-Print Network [OSTI]

    Homer, Nils; Nelson, Stanley F; Merriman, Barry

    2010-01-01T23:59:59.000Z

    k-base encoded DNA sequence BMC Bioinformatics 2010, 11:347similarities in the amino acid sequence of two proteins. Jof two-base encoded DNA sequence. BMC Bioinformatics 2009,

  16. Flow cytometric measurement of total DNA and incorporated halodeoxyuridine

    DOE Patents [OSTI]

    Dolbeare, F.A.; Gray, J.W.

    1983-10-18T23:59:59.000Z

    A method for the simultaneous flow cylometric measurement of total cellular DNA content and of the uptake of DNA precursors as a measure of DNA synthesis during various phases of the cell cycle in normal and malignant cells in vitro and in vivo is described. The method comprises reacting cells with labelled halodeoxyuridine (HdU), partially denaturing cellular DNA, adding to the reaction medium monoclonal antibodies (mabs) reactive with HdU, reacting the bound mabs with a second labelled antibody, incubating the mixture with a DNA stain, and measuring simultaneously the intensity of the DNA stain as a measure of the total cellular DNA and the HdU incorporated as a measure of DNA synthesis. (ACR)

  17. Photoelectrochemical array platform for genomic scale DNA synthesis

    E-Print Network [OSTI]

    Emig, Christopher Joseph

    2006-01-01T23:59:59.000Z

    Molecular and synthetic biologists have increasing demand for large, high-fidelity constructs of synthetic DNA. Recent developments in harvesting oligonucleotides from DNA microarrays has proven that these can be assembled ...

  18. Enhancement of in vitro Translation by Gold Nanoparticle – DNA Conjugates

    E-Print Network [OSTI]

    Park, Sunho

    Gold nanoparticle (AuNP)?DNA conjugates can enhance in vitro translation of a protein. Enhancement occurs via a combination of nonspecific adsorption of translation-related molecules and the ribosome to the AuNP?DNA and ...

  19. Digital Actuator Technology

    SciTech Connect (OSTI)

    Ken Thomas; Ted Quinn; Jerry Mauck; Richard Bockhorst

    2014-09-01T23:59:59.000Z

    There are significant developments underway in new types of actuators for power plant active components. Many of these make use of digital technology to provide a wide array of benefits in performance of the actuators and in reduced burden to maintain them. These new product offerings have gained considerable acceptance in use in process plants. In addition, they have been used in conventional power generation very successfully. This technology has been proven to deliver the benefits promised and substantiate the claims of improved performance. The nuclear industry has been reluctant to incorporate digital actuator technology into nuclear plant designs due to concerns due to a number of concerns. These could be summarized as cost, regulatory uncertainty, and a certain comfort factor with legacy analog technology. The replacement opportunity for these types of components represents a decision point for whether to invest in more modern technology that would provide superior operational and maintenance benefits. Yet, the application of digital technology has been problematic for the nuclear industry, due to qualification and regulatory issues. With some notable exceptions, the result has been a continuing reluctance to undertake the risks and uncertainties of implementing digital actuator technology when replacement opportunities present themselves. Rather, utilities would typically prefer to accept the performance limitations of the legacy analog actuator technologies to avoid impacts to project costs and schedules. The purpose of this report is to demonstrate that the benefits of digital actuator technology can be significant in terms of plant performance and that it is worthwhile to address the barriers currently holding back the widespread development and use of this technology. It addresses two important objectives in pursuit of the beneficial use of digital actuator technology for nuclear power plants: 1. To demonstrate the benefits of digital actuator technology over legacy analog sensor technology in both quantitative and qualitative ways. 2. To recognize and address the added difficulty of digital technology qualification, especially in regard to software common cause failure (SCCF), that is introduced by the use of digital actuator technology.

  20. Technology Catalogue. First edition

    SciTech Connect (OSTI)

    Not Available

    1994-02-01T23:59:59.000Z

    The Department of Energy`s Office of Environmental Restoration and Waste Management (EM) is responsible for remediating its contaminated sites and managing its waste inventory in a safe and efficient manner. EM`s Office of Technology Development (OTD) supports applied research and demonstration efforts to develop and transfer innovative, cost-effective technologies to its site clean-up and waste management programs within EM`s Office of Environmental Restoration and Office of Waste Management. The purpose of the Technology Catalogue is to provide performance data on OTD-developed technologies to scientists and engineers assessing and recommending technical solutions within the Department`s clean-up and waste management programs, as well as to industry, other federal and state agencies, and the academic community. OTD`s applied research and demonstration activities are conducted in programs referred to as Integrated Demonstrations (IDs) and Integrated Programs (IPs). The IDs test and evaluate.systems, consisting of coupled technologies, at specific sites to address generic problems, such as the sensing, treatment, and disposal of buried waste containers. The IPs support applied research activities in specific applications areas, such as in situ remediation, efficient separations processes, and site characterization. The Technology Catalogue is a means for communicating the status. of the development of these innovative technologies. The FY93 Technology Catalogue features technologies successfully demonstrated in the field through IDs and sufficiently mature to be used in the near-term. Technologies from the following IDs are featured in the FY93 Technology Catalogue: Buried Waste ID (Idaho National Engineering Laboratory, Idaho); Mixed Waste Landfill ID (Sandia National Laboratories, New Mexico); Underground Storage Tank ID (Hanford, Washington); Volatile organic compound (VOC) Arid ID (Richland, Washington); and VOC Non-Arid ID (Savannah River Site, South Carolina).

  1. College of Information Technology 151 INFORMATION TECHNOLOGY

    E-Print Network [OSTI]

    Xie,Jiang (Linda)

    the computer industry either as a computer hardware design engineer or as a computer scientist with a heavy, industry professionals, and students. The College of IT was formed in 2000, with the mission of educating industry to develop information technology solutions. Computer Science Program. The Computer Science

  2. PAVEMENT TECHNOLOGY UPDATE This Technology Transfer Program

    E-Print Network [OSTI]

    California at Berkeley, University of

    the road." In recent years, increasing amounts of crumb rubber from recycled tires have been added solve the very serious problem of waste tire disposal. TECHNOLOGY TRANSFER PROGRAM SEPTEMBER 2009, VOL. 1, NO. 2 § Rubber Roads: Waste Tires Find a Home By Larry Santucci, PE Pavement Specialist

  3. Testing Technology: A Sandia technology bulletin

    SciTech Connect (OSTI)

    Goetsch, B.; Floyd, H.L.; Doran, L. [eds.

    1994-08-01T23:59:59.000Z

    Inside this issue is a farewell to Testing Technology message from technical advisor, Ruth David. Also included are articles on: Testing the I-40 bridge over the Rio Grande, simulated reactor meltdown studies, an inexpensive monitor for testing integrated circuits, testing of antihelicoptor mines, and quality assurance on aircraft inspection.

  4. An Investigation on Gel Electrophoresis with Quantum Dots End-labeled DNA

    E-Print Network [OSTI]

    Chen, Xiaojia

    2009-05-15T23:59:59.000Z

    explored manipulating DNA fragments by end labeling DNA molecules with quantum dot nanocrystals. The quantum dot-DNA conjugates can be further modified through binding interactions with biotinylated single-stranded DNA primers. Single molecule visualization...

  5. RhoJ Regulates Melanoma Chemoresistance by Suppressing Pathways that Sense DNA Damage

    E-Print Network [OSTI]

    Ho, Hsiang; Aruri, Jayavani; Kapadia, Rubina; Mehr, Hootan; White, Michael A.; Ganesan, Anand K.

    2012-01-01T23:59:59.000Z

    Pathways that Sense DNA Damage $watermark-text Hsiang Ho 1 ,16. Roos WP, Kaina B. DNA damage-induced apoptosis: FromDNA lesions to the DNA damage response and apoptosis. Cancer

  6. A User's Guide to the Encyclopedia of DNA Elements (ENCODE)

    E-Print Network [OSTI]

    Bernstein, Bradley E.

    The mission of the Encyclopedia of DNA Elements (ENCODE) Project is to enable the scientific and medical communities to

  7. Beyond DNA origami: the unfolding prospects of

    E-Print Network [OSTI]

    Walter, Nils G.

    for functional nanodevices. Not limited to nucleic acids, these nanodevices can incorporate other structural and functional materials, such as proteins and nanoparticles, making them broadly useful for current and futureOpinion Beyond DNA origami: the unfolding prospects of nucleic acid nanotechnology Nicole

  8. Rewritable Memory by Controllable Nanopatterning of DNA

    E-Print Network [OSTI]

    Pierce, Niles A.

    ABSTRACT Fabricating a nanostructure capable of reversibly patterning molecules is a fundamental goal within nanotechnology, underlying diverse processes such as information storage, scaffold functioning of the device as rewritable memory. The bit state of each address is controlled by specific DNA

  9. Department of Energy Technology

    E-Print Network [OSTI]

    Risa-R-482 Department of Energy Technology Annual Progress Report 1 January - 31 December 1982 Ris.1. The Department of Energy Technology 5 1.2. System and Reliability Analysis 6 1.3. Reactor Physics and Dynamics 7 .. 27 2.10. Severe Accident Analysis 30 2.11. The Advanced BWR Emergency Core Cooling Program NOORCOOL

  10. Gasification: A Cornerstone Technology

    SciTech Connect (OSTI)

    Gary Stiegel

    2008-03-26T23:59:59.000Z

    NETL is a leader in the science and technology of gasification - a process for the conversion of carbon-based materials such as coal into synthesis gas (syngas) that can be used to produce clean electrical energy, transportation fuels, and chemicals efficiently and cost-effectively using domestic fuel resources. Gasification is a cornerstone technology of 21st century zero emissions powerplants

  11. Gasification: A Cornerstone Technology

    ScienceCinema (OSTI)

    Gary Stiegel

    2010-01-08T23:59:59.000Z

    NETL is a leader in the science and technology of gasification - a process for the conversion of carbon-based materials such as coal into synthesis gas (syngas) that can be used to produce clean electrical energy, transportation fuels, and chemicals efficiently and cost-effectively using domestic fuel resources. Gasification is a cornerstone technology of 21st century zero emissions powerplants

  12. IITB TECHNOLOGIES DIRECTOR'S MESSAGE

    E-Print Network [OSTI]

    Narayanan, H.

    is useful to companies who are looking to commercialise new technologies and bring them to the market place technologies under different themes of Healthcare, Energy & Environment, Information & Communication of Microelectronics Lab #12;INDEX 1 Healthcare 1.1 An Enlightening Device for Visually Impaired People

  13. Reprogramming DNA Methylation in Bovine Cells by Knocking Down DNA Methyltransferase-1 with RNA Interference 

    E-Print Network [OSTI]

    Stroud, Todd

    2010-01-20T23:59:59.000Z

    Embryos derived by somatic cell nuclear transfer (SCNT) produce few pregnancies that result in a live, healthy offspring. This has largely been attributed to the aberrant reprogramming of the somatic cell DNA used for ...

  14. DNA Strands Attached Inside Single Conical Nanopores: Ionic Pore Characteristics and Insight into DNA Biophysics

    E-Print Network [OSTI]

    Nguyen, Gael; Howorka, Stefan; Siwy, Zuzanna S.

    2011-01-01T23:59:59.000Z

    Kathawalla et al. 1989; Heng et al. 2005; Keyser et al.due to the electric ?eld (Heng et al. 2005; Randall et al.DNA analysis. Nanomedicine Heng JB, Aksimentiev A, Ho C,

  15. Structural basis for the inhibition of human alkyladenine DNA by 3,N4-ethenocytosine containing DNA

    E-Print Network [OSTI]

    Lingaraju, Gondichatnahalli M.

    Reactive oxygen and nitrogen species, generated by neutrophils and macrophages in chronically inflamed tissues, readily damage DNA, producing a variety of potentially genotoxic etheno base lesions; such inflammation-related ...

  16. DNA ruler : enhancing nanopore sizing resolution by multiple measurements on the same DNA molecule

    E-Print Network [OSTI]

    Sen, Yi-Heng

    2012-01-01T23:59:59.000Z

    Nanopores are versatile sensors for label-free detection of single molecules and particles that have attracted attention for applications such as DNA sequencing and nanoparticle analysis. Detection of single molecules or ...

  17. PRESSURE ACTIVATED SEALANT TECHNOLOGY

    SciTech Connect (OSTI)

    Michael A. Romano

    2004-04-01T23:59:59.000Z

    The objective of this project is to develop new, efficient, cost effective methods of internally sealing natural gas pipeline leaks through the application of differential pressure activated sealants. In researching the current state of the art for gas pipeline sealing technologies we concluded that if the project was successful, it appeared that pressure activated sealant technology would provide a cost effective alternative to existing pipeline repair technology. From our analysis of current field data for a 13 year period from 1985 to 1997 we were able to identify 205 leaks that were candidates for pressure activated sealant technology, affirming that pressure activated sealant technology is a viable option to traditional external leak repairs. The data collected included types of defects, areas of defects, pipe sizes and materials, incident and operating pressures, ability of pipeline to be pigged and corrosion states. This data, and subsequent analysis, was utilized as a basis for constructing applicable sealant test modeling.

  18. Updating SRM 2391c: PCR-Based DNA Profiling Standard

    E-Print Network [OSTI]

    profiling SRM 2391 SRM 2391a SRM 2391b SRM 2391c SRM 2390 DNA profiling 2391 series PCR-based DNA profiling 40 NMDEPARTMENT OF PUBLIC SAFETY 10 DNASI 40 SEROLOGICAL RESEARCH INSTITUTE 11 CHARLOTTE- DNA CRIME LAB 50 SOUTH CAROLINA LAW ENFORCEMENT 20 ILLUMINA INC CARROLL PARK 50 UWA INTERNATIONAL

  19. A Simple and Fast DNA Compressor Giovanni Manzini

    E-Print Network [OSTI]

    Manzini, Giovanni

    A Simple and Fast DNA Compressor Giovanni Manzini Marcella Rastero February 17, 2004 Abstract. For this reason most DNA compressors work by searching and encoding approximate repeats. We depart from to the best DNA compressors. Another important feature of our algorithm is its small space occupancy which

  20. DNA overwinds when stretched , Zev Bryant2,4

    E-Print Network [OSTI]

    Gore, Jeff

    DNA overwinds when stretched Jeff Gore1 , Zev Bryant2,4 , Marcelo NoØllmann2 , Mai U. Le2 , Nicholas R. Cozzarelli2 & Carlos Bustamante1­4 DNA is often modelled as an isotropic rod1­4 , but its coupling between twisting and stretch- ing degrees of freedom. Simple physical intuition predicts that DNA

  1. The DNA binding activity of p53 displays reactiondiffusion kinetics

    E-Print Network [OSTI]

    Hinow, Peter

    The DNA binding activity of p53 displays reaction­diffusion kinetics 26th Southeastern 37240 The DNA binding activity of p53 displays reaction­diffusion kinetics ­ p. 1/2 #12;Collaborators, Vanderbilt University · Emmanuele DiBenedetto, PhD, Department of Mathematics, Vanderbilt University The DNA

  2. DNA methylation and the analysis of CpG Islands

    E-Print Network [OSTI]

    Czygrinow, Andrzej

    DNA methylation and the analysis of CpG Islands in genomes M. F. Wojciechowski MAT 351 25 March 2005 #12;#12;Nucleotides #12;Base pairing * * #12;DNA methylation In mammalian genomes, methylation residues represent a target for covalent modification of DNA Cytosine is one of two bases found commonly

  3. DNA Nanotechnology DOI: 10.1002/anie.201206389

    E-Print Network [OSTI]

    Hone, James

    DNA Nanotechnology DOI: 10.1002/anie.201206389 Assembly of Heterogeneous Functional Nanomaterials on DNA Origami Scaffolds** Risheng Wang,* Colin Nuckolls, and Shalom J. Wind* Hybrid nanomaterial systems;[1] selective growth;[4,9] and DNA-mediated assem- bly,[3,8] including the formation of 3D

  4. DNA IN THE UK By Erica Van Coverden

    E-Print Network [OSTI]

    Goodwin, Kelly D.

    DNA IN THE UK By Erica Van Coverden Dr. Kelly Goodwin, a microbiologist at AOML, finished up. Those proteins then would be sequenced and the amino acid sequence would be used to backtrack to the DNA sequence that codes for that protein. In another approach, total DNA was extracted, digested

  5. Methods to alter levels of a DNA repair protein

    DOE Patents [OSTI]

    Petrini, John H.; Morgan, William Francis; Maser, Richard Scott; Carney, James Patrick

    2006-10-17T23:59:59.000Z

    An isolated and purified DNA molecule encoding a DNA repair protein, p95, is provided, as is isolated and purified p95. Also provided are methods of detecting p95 and DNA encoding p95. The invention further provides p95 knock-out mice.

  6. Reaction Temperature Constraints in DNA Computing Russell Deaton

    E-Print Network [OSTI]

    Deaton, Russell J.

    Reaction Temperature Constraints in DNA Computing Russell Deaton The Department of Electrical the thermodynamics of DNA melt- ing, a technique is proposed to choose a re- action temperature for the DNA computa the melting temperature, Tm. The melting temperature is determined from curves of UV absorbance versus

  7. Recurrence time statistics: Versatile tools for genomic DNA sequence analysis

    E-Print Network [OSTI]

    Gao, Jianbo

    Recurrence time statistics: Versatile tools for genomic DNA sequence analysis Yinhe Cao1, Wen, and the genomes of many other organisms waiting to be sequenced, it has become increasingly important to develop from DNA sequences. One of the more important structures in a DNA se- quence is repeat-related. Often

  8. Correct and incorrect nucleotide incorporation pathways in DNA polymerase b

    E-Print Network [OSTI]

    Schlick, Tamar

    Correct and incorrect nucleotide incorporation pathways in DNA polymerase b Ravi Radhakrishnan a nucleotide incorporations in the DNA by using a novel protocol involving energy minimizations, dynamics simu- sive transient intermediates, for nucleotide incorporation at the template/primer DNA junction. A large

  9. Technology Strategic Plan 2013 2016 Office of Information Technology

    E-Print Network [OSTI]

    Brinkmann, Peter

    Technology Strategic Plan 2013 ­ 2016 Office of Information Technology June 2013 #12;2 T A B L E O F C O N T E N T S: - Introduction - - Executive Summary - - Terminology - - A Vision for Technology at the City College of New York - - The Mission of the Office for Technology (OIT) - - Technology Guiding

  10. TECHNOLOGY INNOVATION PROGRAM National Institute of Standards and Technology

    E-Print Network [OSTI]

    Magee, Joseph W.

    TECHNOLOGY INNOVATION PROGRAM National Institute of Standards and Technology Gaithersburg, MD 20899 ADVANCED TECHNOLOGIES FOR CIVIL INFRASTRUCTURE The Technology Innovation Program (TIP) at the National Institute of Standards and Technology was established to assist U.S. businesses and institutions of higher

  11. Information Technology and Management Department of Information Technology and Management

    E-Print Network [OSTI]

    Heller, Barbara

    Information Technology and Management Department of Information Technology and Management Daniel F Chicago, IL 60616 appliedtech.iit.edu/information-technology-and- management Dean and Chair: C. Robert in the information technology and cyber security fields. The Information Technology and Management degrees apply

  12. Structure of the SSB?DNA polymerase III interface and its role in DNA replication

    SciTech Connect (OSTI)

    Marceau, Aimee H.; Bahng, Soon; Massoni, Shawn C.; George, Nicholas P.; Sandler, Steven J.; Marians, Kenneth J.; Keck, James L. (MSKCC); (UMASS, Amherst); (UW-MED)

    2012-05-22T23:59:59.000Z

    Interactions between single-stranded DNA-binding proteins (SSBs) and the DNA replication machinery are found in all organisms, but the roles of these contacts remain poorly defined. In Escherichia coli, SSB's association with the {chi} subunit of the DNA polymerase III holoenzyme has been proposed to confer stability to the replisome and to aid delivery of primers to the lagging-strand DNA polymerase. Here, the SSB-binding site on {chi} is identified crystallographically and biochemical and cellular studies delineate the consequences of destabilizing the {chi}/SSB interface. An essential role for the {chi}/SSB interaction in lagging-strand primer utilization is not supported. However, sequence changes in {chi} that block complex formation with SSB lead to salt-dependent uncoupling of leading- and lagging-strand DNA synthesis and to a surprising obstruction of the leading-strand DNA polymerase in vitro, pointing to roles for the {chi}/SSB complex in replisome establishment and maintenance. Destabilization of the {chi}/SSB complex in vivo produces cells with temperature-dependent cell cycle defects that appear to arise from replisome instability.

  13. Can we model DNA at the mesoscale ? Comment on: Fluctuations in the DNA double helix: A critical review

    E-Print Network [OSTI]

    Peyrard, Michel

    2015-01-01T23:59:59.000Z

    Comment on "Fluctuations in the DNA double helix: A critical review" by Frank-Kamenetskii and Prakash

  14. DNA digestion protocol & hints Overview: Although it is pretty standard to digest DNA with restriction enzymes, here

    E-Print Network [OSTI]

    Doering, Tamara

    Liu 4/2004 DNA digestion protocol & hints Overview: Although it is pretty standard to digest DNA in molecular biology (3.1.1-3.1.2) Materials: · DNA sample in water or TE buffer · 10x digestion buffer.1 to 4 µg 10x Digestion buffer 2 µl 5 µl Enzyme ? ? Water Rest of volume Rest of volume 2. Add the enzyme

  15. The IDA Technology Stan Franklin

    E-Print Network [OSTI]

    Memphis, University of

    The IDA Technology Stan Franklin and the `Conscious' Software Research Group #12;FedEx Institute of Technology--The IDA Technology 2 Introducing IDA An intelligent software agent capable of entirely of Technology--The IDA Technology 3 IDA Negotiates IDA negotiates with clients in natural language

  16. Technology Innovation Program Advisory Board

    E-Print Network [OSTI]

    Magee, Joseph W.

    Technology Innovation Program Advisory Board 2009 Annual Report of the #12;2009 Annual Report of the Technology Innovation Program Advisory Board U.S. Department of Commerce National Institute of Standards and Technology Technology Innovation Program February 2010 #12;For Information regarding the Technology

  17. Technology Innovation Program Advisory Board

    E-Print Network [OSTI]

    Technology Innovation Program Advisory Board 2011 Annual Report of the #12;#12;i 2011 Annual Report of the Technology Innovation Program Advisory Board U.S. Department of Commerce National Institute of Standards and Technology Technology Innovation Program March 2012 #12;ii For Information regarding the Technology

  18. Indian Institute of Technology Bombay INDIAN INSTITUTE OF TECHNOLOGY BOMBAY

    E-Print Network [OSTI]

    Narayanan, H.

    Indian Institute of Technology Bombay INDIAN INSTITUTE OF TECHNOLOGY BOMBAY INVITATION Description of work Estimated cost (1) (2) (3) 1 Construction of Institutional/Residential buildings, external development, HVAC, Elevators etc. for Indian Institute of Technology Bombay, at the campus

  19. COPPER CABLE RECYCLING TECHNOLOGY

    SciTech Connect (OSTI)

    Chelsea Hubbard

    2001-05-01T23:59:59.000Z

    The United States Department of Energy (DOE) continually seeks safer and more cost-effective technologies for use in deactivation and decommissioning (D&D) of nuclear facilities. The Deactivation and Decommissioning Focus Area (DDFA) of the DOE's Office of Science and Technology (OST) sponsors large-scale demonstration and deployment projects (LSDDPs). At these LSDDPs, developers and vendors of improved or innovative technologies showcase products that are potentially beneficial to the DOE's projects and to others in the D&D community. Benefits sought include decreased health and safety risks to personnel and the environment, increased productivity, and decreased costs of operation. The Idaho National Engineering and Environmental Laboratory (INEEL) generated a list of statements defining specific needs and problems where improved technology could be incorporated into ongoing D&D tasks. One such need is to reduce the volume of waste copper wire and cable generated by D&D. Deactivation and decommissioning activities of nuclear facilities generates hundreds of tons of contaminated copper cable, which are sent to radioactive waste disposal sites. The Copper Cable Recycling Technology separates the clean copper from contaminated insulation and dust materials in these cables. The recovered copper can then be reclaimed and, more importantly, landfill disposal volumes can be reduced. The existing baseline technology for disposing radioactively contaminated cables is to package the cables in wooden storage boxes and dispose of the cables in radioactive waste disposal sites. The Copper Cable Recycling Technology is applicable to facility decommissioning projects at many Department of Energy (DOE) nuclear facilities and commercial nuclear power plants undergoing decommissioning activities. The INEEL Copper Cable Recycling Technology Demonstration investigated the effectiveness and efficiency to recycle 13.5 tons of copper cable. To determine the effectiveness of separating out radioactive contamination, the copper cable was coated with a surrogate contaminant. The demonstration took place at the Bonneville County Technology Center in Idaho Falls, Idaho.

  20. Preparation of DNA-containing extract for PCR amplification

    DOE Patents [OSTI]

    Dunbar, John M.; Kuske, Cheryl R.

    2006-07-11T23:59:59.000Z

    Environmental samples typically include impurities that interfere with PCR amplification and DNA quantitation. Samples of soil, river water, and aerosol were taken from the environment and added to an aqueous buffer (with or without detergent). Cells from the sample are lysed, releasing their DNA into the buffer. After removing insoluble cell components, the remaining soluble DNA-containing extract is treated with N-phenacylthiazolium bromide, which causes rapid precipitation of impurities. Centrifugation provides a supernatant that can be used or diluted for PCR amplification of DNA, or further purified. The method may provide a DNA-containing extract sufficiently pure for PCR amplification within 5–10 minutes.

  1. Stepped electrophoresis for movement and concentration of DNA

    DOE Patents [OSTI]

    Miles, Robin R.; Wang, Amy Wei-Yun; Mariella, Jr., Raymond P.

    2005-03-15T23:59:59.000Z

    A fluidic channel patterned with a series of thin-film electrodes makes it possible to move and concentrate DNA in a fluid passing through the fluidic channel. The DNA has an inherent negative charge and by applying a voltage between adjacent electrodes the DNA is caused to move. By using a series of electrodes, when one electrode voltage or charge is made negative with respect to adjacent electrodes, the DNA is repelled away from this electrode and attached to a positive charged electrode of the series. By sequentially making the next electrode of the series negative, the DNA can be moved to and concentrated over the remaining positive electrodes.

  2. T4 DNA condensation in water-alcohol media

    E-Print Network [OSTI]

    M. O. Gallyamov; O. A. Pyshkina; V. G. Sergeyev; I. V. Yaminsky

    2011-07-21T23:59:59.000Z

    The process of compaction of high molecular weight DNA T4 is investigated directly in a AFM liquid cell. The AFM-images of globules formed by DNA molecules in the result of compaction in water-alcohol environments at high izopropanol concentration (80%) are received; it is found that at intermediate concentration of izopropanol (40-50%) the DNA molecules form partially compacted formations in which the separate coils of macromolecules twist in toroidal structures. It is shown using the technique of deconvolution of the AFM-images that the globule include only one closely packed DNA molecule. The model of DNA packing is proposed on the basis of AFM experiment.

  3. DNA-nuclear matrix interactions and ionizing radiation sensitivity

    SciTech Connect (OSTI)

    Schwartz, J.L. (Argonne National Lab., IL (United States) Chicago Univ., IL (United States). Dept. of Radiation and Cellular Oncology); Vaughan, A.T.M. (Loyola Univ., Hines, IL (United States). Dept. of Radiotherapy)

    1993-01-01T23:59:59.000Z

    The association between inherent ionizing radiation sensitivity and DNA supercoil unwinding in mammalian cells suggests that the DNA-nuclear matrix attachment region (MAR) plays an important role in radiation response. In radioresistant cells, the MAR structure may exist in a more stable, open configuration, limiting DNA unwinding following strand break induction and maintaining DNA ends in close proximity for more rapid and accurate rejoining. In addition, the open configuration at these matrix attachment sites may serve to facilitate rapid DNA processing of breaks by providing (1) sites for repair proteins to collect and (2) energy to drive enzymatic reactions.

  4. DNA-nuclear matrix interactions and ionizing radiation sensitivity

    SciTech Connect (OSTI)

    Schwartz, J.L. [Argonne National Lab., IL (United States)]|[Chicago Univ., IL (United States). Dept. of Radiation and Cellular Oncology; Vaughan, A.T.M. [Loyola Univ., Hines, IL (United States). Dept. of Radiotherapy

    1993-03-01T23:59:59.000Z

    The association between inherent ionizing radiation sensitivity and DNA supercoil unwinding in mammalian cells suggests that the DNA-nuclear matrix attachment region (MAR) plays an important role in radiation response. In radioresistant cells, the MAR structure may exist in a more stable, open configuration, limiting DNA unwinding following strand break induction and maintaining DNA ends in close proximity for more rapid and accurate rejoining. In addition, the open configuration at these matrix attachment sites may serve to facilitate rapid DNA processing of breaks by providing (1) sites for repair proteins to collect and (2) energy to drive enzymatic reactions.

  5. Robotics Technology Development Program. Technology summary

    SciTech Connect (OSTI)

    Not Available

    1994-02-01T23:59:59.000Z

    The Robotics Technology Development Program (RTDP) is a ``needs-driven`` effort. A lengthy series of presentations and discussions at DOE sites considered critical to DOE`s Environmental Restoration and Waste Management (EM) Programs resulted in a clear understanding of needed robotics applications toward resolving definitive problems at the sites. A detailed analysis of the Tank Waste Retrieval (TWR), Contaminant Analysis Automation (CAA), Mixed Waste Operations (MWO), and Decontamination & Dismantlement (D&D). The RTDP Group realized that much of the technology development was common (Cross Cutting-CC) to each of these robotics application areas, for example, computer control and sensor interface protocols. Further, the OTD approach to the Research, Development, Demonstration, Testing, and Evaluation (RDDT&E) process urged an additional organizational break-out between short-term (1--3 years) and long-term (3--5 years) efforts (Advanced Technology-AT). The RDTP is thus organized around these application areas -- TWR, CAA, MWO, D&D and CC&AT -- with the first four developing short-term applied robotics. An RTDP Five-Year Plan was developed for organizing the Program to meet the needs in these application areas.

  6. OHVT technology roadmap [2000

    SciTech Connect (OSTI)

    Bradley, R.A.

    2000-02-01T23:59:59.000Z

    The OHVT Technology Roadmap for 2000 presents the multiyear program plan of the U.S. DOE's Office of Heavy Vehicle Technologies (OHVT). It is an update of the 1997 plan, reflecting changes in regulations and ongoing discussions with DOE's heavy vehicle customers. The technical plan covers three classes of trucks: (1) class 7-8 (large, on-highway trucks); (2) class 3-6 (medium duty trucks); (3) class 1-2 (pickups, vans, and sport utility vehicles) as well as enabling and supporting technologies. The Roadmap documents program goals, schedules, and milestones.

  7. DNA end resection by Dna2Sgs1RPA and its stimulation by Top3Rmi1 and Mre11Rad50Xrs2

    E-Print Network [OSTI]

    Kowalczykowski, Stephen C.

    LETTERS DNA end resection by Dna2­Sgs1­RPA and its stimulation by Top3­Rmi1 and Mre11­Rad50­Xrs2. Campbell3 & Stephen C. Kowalczykowski1,2 The repair of DNA double-strand breaks (DSBs) by homologous to generate a 39-single- stranded DNA (ssDNA) overhang, which becomes a substrate forthe

  8. High energy density capacitors using nano-structure multilayer technology

    SciTech Connect (OSTI)

    Barbee, T.W. Jr.; Johnson, G.W.; O`Brien, D.W.

    1992-08-01T23:59:59.000Z

    Today, many pulse power and industrial applications are limited by capacitor performance. While incremental improvements are anticipated from existing capacitor technologies, significant advances are needed in energy density to enable these applications for both the military and for American economic competitiveness. We propose a program to research and develop a novel technology for making high voltage, high energy density capacitors. Nano-structure multilayer technologies developed at LLNL may well provide a breakthrough in capacitor performance. Our controlled sputtering techniques are capable of laying down extraordinarily smooth sub-micron layers of dielectric and conductor materials. With this technology, high voltage capacitors with an order of magnitude improvement in energy density may be achievable. Well-understood dielectrics and new materials will be investigated for use with this technology. Capacitors developed by nano-structure multilayer technology are inherently solid state, exhibiting extraordinary mechanical and thermal properties. The conceptual design of a Notepad capacitor is discussed to illustrate capacitor and capacitor bank design and performance with this technology. We propose a two phase R&D program to address DNA`s capacitor needs for electro-thermal propulsion and similar pulse power programs. Phase 1 will prove the concept and further our understanding of dielectric materials and design tradeoffs with multilayers. Nano-structure multilayer capacitors will be developed and characterized. As our materials research and modeling prove successful, technology insertion in our capacitor designs will improve the possibility for dramatic performance improvements. In Phase 2, we will make Notepad capacitors, construct a capacitor bank and demonstrate its performance in a meaningful pulse power application. We will work with industrial partners to design full scale manufacturing and move this technology to industry for volume production.

  9. DOE Vehicle Technologies Program 2009 Merit Review Report - Technology...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Review Report DOE Vehicle Technologies Program 2009 Merit Review Report - Vehicle Systems DOE Vehicle Technologies Program 2009 Merit Review Report - Safety Codes and Standards...

  10. MHK Technologies/Oregon State University Columbia Power Technologies...

    Open Energy Info (EERE)

    Jump to: navigation, search << Return to the MHK database homepage Oregon State University Columbia Power Technologies Direct Drive Point Absorber.jpg Technology Profile...

  11. Technology Readiness Assessment (TRA)/Technology Maturation Plan...

    Broader source: Energy.gov (indexed) [DOE]

    is a guide for those involved in conducting TRAs and developing TMPs for DOE-EM. Technology Readiness Assessment (TRA)Technology Maturation Plan (TMP) Process Guide More...

  12. National Wind Technology Center (Fact Sheet), National Wind Technology...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    NATIONAL WIND TECHNOLOGY CENTER www.nrel.govwind Wind energy is one of the fastest growing electricity generation sources in the world. NREL's National Wind Technology Center...

  13. Long DNA molecule as a pseudoscalar liquid crystal

    E-Print Network [OSTI]

    K. G. Petrosyan; Chin-Kun Hu

    2009-05-13T23:59:59.000Z

    We show that a long DNA molecule can form a novel condensed phase of matter, the pseudoscalar liquid crystal, that consists of aperiodically ordered DNA fragments in right-handed B and left-handed Z forms. We discuss the possibility of transformation of B-DNA into Z-DNA and vice versa via first-order phase transitions as well as transformations from the phase with zero total chirality into pure B- or Z-DNA samples through second-order phase transitions. The presented minimalistic phenomenological model describes the pseudoscalar liquid crystal phase of DNA and the phase transition phenomena. We point out to a possibility that a pseudoscalar liquid nano-crystal can be assembled via DNA-programming.

  14. Distinct kinetics of human DNA ligases I, IIIalpha, IIIbeta, and IV reveal direct DNA sensing ability and differential physiological functions in DNA repair

    SciTech Connect (OSTI)

    Chen, Xi; Ballin, Jeff D.; Della-Maria, Julie; Tsai, Miaw-Sheue; White, Elizabeth J.; Tomkinson, Alan E.; Wilson, Gerald M.

    2009-05-11T23:59:59.000Z

    The three human LIG genes encode polypeptides that catalyze phosphodiester bond formation during DNA replication, recombination and repair. While numerous studies have identified protein partners of the human DNA ligases (hLigs), there has been little characterization of the catalytic properties of these enzymes. In this study, we developed and optimized a fluorescence-based DNA ligation assay to characterize the activities of purified hLigs. Although hLigI joins DNA nicks, it has no detectable activity on linear duplex DNA substrates with short, cohesive single-strand ends. By contrast, hLigIII{beta} and the hLigIII{alpha}/XRCC1 and hLigIV/XRCC4 complexes are active on both nicked and linear duplex DNA substrates. Surprisingly, hLigIV/XRCC4, which is a key component of the major non-homologous end joining (NHEJ) pathway, is significantly less active than hLigIII on a linear duplex DNA substrate. Notably, hLigIV/XRCC4 molecules only catalyze a single ligation event in the absence or presence of ATP. The failure to catalyze subsequent ligation events reflects a defect in the enzyme-adenylation step of the next ligation reaction and suggests that, unless there is an in vivo mechanism to reactivate DNA ligase IV/XRCC4 following phosphodiester bond formation, the cellular NHEJ capacity will be determined by the number of adenylated DNA ligaseIV/XRCC4 molecules.

  15. Wind Technologies & Evolving Opportunities (Presentation)

    SciTech Connect (OSTI)

    Robichaud, R.

    2014-07-01T23:59:59.000Z

    This presentation covers opportunities for wind technology; wind energy market trends; an overview of the National Wind Technology Center near Boulder, Colorado; wind energy price and cost trends; wind turbine technology improvements; and wind resource characterization improvements.

  16. Water Management Technologies from Europe

    E-Print Network [OSTI]

    Woinsky, S. G.

    concentration technology are described. It is anticipated that when appropriate, the EPRI/Pell Frischmann/ BG Technology cooperation will be extended to include unbiased evaluation of such third party technologies....

  17. Sandia Energy - A Green Technology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    A Green Technology Home Energy Research EFRCs Solid-State Lighting Science EFRC A Green Technology A Green TechnologyTara Camacho-Lopez2015-05-11T21:08:32+00:00 Solid-State...

  18. February 2000 Advanced Technology Program

    E-Print Network [OSTI]

    of Standards and Technology (NIST) is a cost-sharing program designed to partner the federal governmentFebruary 2000 Advanced Technology Program Information Infrastructure for Healthcare Focused Program: A Brief History ADADVANCEDANCED TECHNOLOGY PRTECHNOLOGY PROGRAMOGRAM NISTIR 6477 National Institute

  19. The Vehicle Technologies Market Report

    E-Print Network [OSTI]

    The Vehicle Technologies Market Report Center for Transportation Analysis 2360 Cherahala Boulevard Efficiency Transportation: Energy Environment Safety Security Vehicle Technologies T he Oak Ridge National Laboratory's Center for Transportation Analysis developed and published the first Vehicle Technologies Market

  20. Overview and Burning Technology Opportunities

    E-Print Network [OSTI]

    Waller, J.

    of technologies in various stages of development ranging from laboratory scale models to old conversion technologies nearly forgotten by this modern day high technology society. I have identified in Table 1, for various biomass feedstocks, the barriers...

  1. Stimulating Energy Technology Innovation

    E-Print Network [OSTI]

    Moniz, Ernest J.

    The innovation system has interrelated components of invention, translation, adoption, and diffusion. Energy technology innovation has lagged that in other domains, and there is a compelling public interest in picking up ...

  2. Technology catalogue. Second edition

    SciTech Connect (OSTI)

    NONE

    1995-04-01T23:59:59.000Z

    The Department of Energy`s (DOE`s) Office of Environmental Management (EM) is responsible for remediating DOE contaminated sites and managing the DOE waste inventory in a safe and efficient manner. EM`s Office of Technology Development (OTD) supports applied research and demonstration efforts to develop and transfer innovative, cost-effective technologies to its site clean-up and waste-management programs within EM. The purpose of the Technology Catalogue is to: (a) provide performance data on OTD-developed technologies to scientists and engineers responsible for preparing Remedial Investigation/Feasibility Studies (RI/FSs) and other compliance documents for the DOE`s clean-up and waste-management programs; and (b) identify partnering and commercialization opportunities with industry, other federal and state agencies, and the academic community.

  3. Marketing Cool Storage Technology 

    E-Print Network [OSTI]

    McCannon, L.

    1987-01-01T23:59:59.000Z

    in the field. The International Thermal Storage Advisory Council was formed to help meet this perceived need. This paper will review activities of EPRI and ITSAC to achieve widespread acceptance of the technology....

  4. Photonic quantum technologies

    E-Print Network [OSTI]

    Jeremy L. O'Brien; Akira Furusawa; Jelena Vu?kovi?

    2010-03-20T23:59:59.000Z

    The first quantum technology, which harnesses uniquely quantum mechanical effects for its core operation, has arrived in the form of commercially available quantum key distribution systems that achieve enhanced security by encoding information in photons such that information gained by an eavesdropper can be detected. Anticipated future quantum technologies include large-scale secure networks, enhanced measurement and lithography, and quantum information processors, promising exponentially greater computation power for particular tasks. Photonics is destined for a central role in such technologies owing to the need for high-speed transmission and the outstanding low-noise properties of photons. These technologies may use single photons or quantum states of bright laser beams, or both, and will undoubtably apply and drive state-of-the-art developments in photonics.

  5. Insider protection technology developments

    SciTech Connect (OSTI)

    Foesch, J.; Bortniak, P.; Waddoups, I.

    1994-08-01T23:59:59.000Z

    Sandia National Laboratories evaluates and develops new techniques and technologies to ensure the integrity of special nuclear material (SNM) against potential insider threats. We have evaluated several types of sensor technologies and subsystems to monitor and/or track materials and personnel. This past year`s effort has been directed at characterizing commercial developments that meet the Department of Energy`s (DOE) needs in some of these areas. Some of these evaluations are complete and some are still in progress. This paper discusses our work with infrared light (IR), radio frequency (RF), and RF proximity technologies. After these technologies are judged to be applicable to DOE`s needs, we incorporate them into the generic, real time, personnel tracking and material monitoring system.

  6. COMPUTER SCIENCE INFORMATION TECHNOLOGY

    E-Print Network [OSTI]

    Dunstan, Neil

    COMPUTER SCIENCE and INFORMATION TECHNOLOGY POSTGRADUATE STUDIES 2006 School of Mathematics of Information Systems with Honours Master of Science (Computer Science) Professional Doctorate in Science (Computer Science) PhD (Computer Science) The postgraduate programs in Computer Science and Information

  7. Membrane Technology Workshop

    Broader source: Energy.gov [DOE]

    At the Membrane Technology Workshop (held July 24, 2012, in Rosemont, IL), stakeholders from industry and academia explored the status of membrane research and development (R&D). Participants ...

  8. Geothermal Technologies Newsletter Archives

    Broader source: Energy.gov [DOE]

    Here you'll find past issues of the U.S. Department of Energy's (DOE) Geothermal Technologies program newsletter, which features information about its geothermal research and development efforts....

  9. Technologies | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and diverse range of technologies that have worldwide impact in a variety of fields. Argonne grants licenses for lab-developed intellectual property to existing and start-up...

  10. Director, Vehicle Technologies Office

    Broader source: Energy.gov [DOE]

    This position is located within the Vehicle Technologies Office (VTO), within the Office of Energy Efficiency and Renewable Energy (EERE). The Office reports to the Deputy Assistant Secretary for...

  11. Clean Coal Technology (Indiana)

    Broader source: Energy.gov [DOE]

    A public utility may not use clean coal technology at a new or existing electric generating facility without first applying for and obtaining from the Utility Regulatory Commission a certificate...

  12. Technology Integration Overview

    Broader source: Energy.gov (indexed) [DOE]

    Next-Steps when R&D is completed Roughly 10% of VTP base budget supports Deployment (Technology Introduction) efforts Over 3.5 Billion Gallons of Petroleum Reduction since...

  13. GeoEnergy technology

    SciTech Connect (OSTI)

    NONE

    1980-12-31T23:59:59.000Z

    The goal of the GeoEnergy Technology Program is to improve the understanding and efficiency of energy extraction and conversion from geologic resources, hence maintaining domestic production capability of fossil energy resources and expanding the usage of geothermal energy. The GeoEnergy Technology Program conducts projects for the Department of Energy in four resource areas--coal, oil and gas, synthetic fuels and geothermal energy. These projects, which are conducted collaboratively with private industry and DOE`s Energy Technology Centers, draw heavily on expertise derived from the nuclear weapons engineering capabilities of Sandia. The primary technologies utilized in the program are instrumentation development and application, geotechnical engineering, drilling and well completions, and chemical and physical process research. Studies in all four resource areas are described.

  14. ENERGY EFFICIENCY TECHNOLOGY ROADMAP

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    l d i n g D e s i g n E n v e l o p e R&D Program Summaries Effective, cost competitive solar shingles. Building-integrated photovoltaic (PV) technologies helps make solar power...

  15. Power conversion technologies

    SciTech Connect (OSTI)

    Newton, M. A.

    1997-02-01T23:59:59.000Z

    The Power Conversion Technologies thrust area identifies and sponsors development activities that enhance the capabilities of engineering at Lawrence Livermore National Laboratory (LLNL) in the area of solid- state power electronics. Our primary objective is to be a resource to existing and emerging LLNL programs that require advanced solid-state power electronic technologies.. Our focus is on developing and integrating technologies that will significantly impact the capability, size, cost, and reliability of future power electronic systems. During FY-96, we concentrated our research efforts on the areas of (1) Micropower Impulse Radar (MIR); (2) novel solid-state opening switches; (3) advanced modulator technology for accelerators; (4) compact accelerators; and (5) compact pulse generators.

  16. SCHOOL OF ENGINEERING TECHNOLOGY Surveying Engineering

    E-Print Network [OSTI]

    Thomas, Andrew

    SCHOOL OF ENGINEERING TECHNOLOGY Surveying Engineering Technology practice FOCUSED WHY SURVEYING ENGINEERING TECHNOLOGY? Surveying engineering technology is a practice- focused program that provides students ENGINEERING TECHNOLOGY DEGREE? A graduate with a surveying engineering technology degree can work as a party

  17. Technology Commercialization Program 1991

    SciTech Connect (OSTI)

    Not Available

    1991-11-01T23:59:59.000Z

    This reference compilation describes the Technology Commercialization Program of the Department of Energy, Defense Programs. The compilation consists of two sections. Section 1, Plans and Procedures, describes the plans and procedures of the Defense Programs Technology Commercialization Program. The second section, Legislation and Policy, identifies legislation and policy related to the Program. The procedures for implementing statutory and regulatory requirements are evolving with time. This document will be periodically updated to reflect changes and new material.

  18. Hole interactions with molecular vibrations on DNA

    E-Print Network [OSTI]

    A. Omerzu; M. Licer; T. Mertelj; V. V. Kabanov; D. Mihailovic

    2004-05-13T23:59:59.000Z

    We report on a study of the interactions between holes and molecular vibrations on dry DNA using photoinduced infrared absorption spectroscopy. Laser photoexcited (PE) holes are found to have a room-temperature lifetime in excess of 1 ms, clearly indicating the presence of localization. However, from a quantitative model analysis of the frequency shifts of vibrational modes caused by the PE holes, we find the holevibrational coupling constant to be relatively small, 0.2. This interaction leads to a change in the conformational energy of 0.015 eV, which is too small to cause selftrapping at room temperature. We conclude that, at least in the dry (A) form, DNA is best understood in terms of a double chain of coupled quantum dots arising from the pseudo-random chain sequence of base pairs, in which Anderson localization prevents the formation of a metallic state.

  19. Conditions for positioning of nucleosomes on DNA

    E-Print Network [OSTI]

    Sheinman, Michael

    2015-01-01T23:59:59.000Z

    Positioning of nucleosomes along eukaryotic genomes plays an important role in their organization and regulation. There are many different factors affecting the location of nucleosomes. Some can be viewed as preferential binding of a single nucleosome to different locations along the DNA and some as interactions between neighboring nucleosomes. In this study we analyzed how well nucleosomes are positioned along the DNA as a function of strength of the preferential binding, correlation length of the binding energy landscape, interactions between neighboring nucleosomes and others relevant system properties. We analyze different scenarios: designed energy landscapes and generically disordered ones and derive conditions for good positioning. Using analytic and numerical approaches we find that, even if the binding preferences are very weak, synergistic interplay between the interactions and the binding preferences is essential for a good positioning of nucleosomes, especially on correlated energy landscapes. Ana...

  20. Fast DNA Sequencing via Transverse Electronic Transport

    E-Print Network [OSTI]

    Johan Lagerqvist; Michael Zwolak; Massimiliano Di Ventra

    2006-03-01T23:59:59.000Z

    A rapid and low-cost method to sequence DNA would usher in a revolution in medicine. We propose and theoretically show the feasibility of a protocol for sequencing based on the distributions of transverse electrical currents of single-stranded DNA while it translocates through a nanopore. Our estimates, based on the statistics of these distributions, reveal that sequencing of an entire human genome could be done with very high accuracy in a matter of hours without parallelization, e.g., orders of magnitude faster than present techniques. The practical implementation of our approach would represent a substantial advancement in our ability to study, predict and cure diseases from the perspective of the genetic makeup of each individual.

  1. Nuclear Reactors and Technology

    SciTech Connect (OSTI)

    Cason, D.L.; Hicks, S.C. [eds.

    1992-01-01T23:59:59.000Z

    This publication Nuclear Reactors and Technology (NRT) announces on a monthly basis the current worldwide information available from the open literature on nuclear reactors and technology, including all aspects of power reactors, components and accessories, fuel elements, control systems, and materials. This publication contains the abstracts of DOE reports, journal articles, conference papers, patents, theses, and monographs added to the Energy Science and Technology Database during the past month. Also included are US information obtained through acquisition programs or interagency agreements and international information obtained through the International Energy Agency`s Energy Technology Data Exchange or government-to-government agreements. The digests in NRT and other citations to information on nuclear reactors back to 1948 are available for online searching and retrieval on the Energy Science and Technology Database and Nuclear Science Abstracts (NSA) database. Current information, added daily to the Energy Science and Technology Database, is available to DOE and its contractors through the DOE Integrated Technical Information System. Customized profiles can be developed to provide current information to meet each user`s needs.

  2. Technology's Impact on Production

    SciTech Connect (OSTI)

    Rachel Amann; Ellis Deweese; Deborah Shipman

    2009-06-30T23:59:59.000Z

    As part of a cooperative agreement with the United States Department of Energy (DOE) - entitled Technology's Impact on Production: Developing Environmental Solutions at the State and National Level - the Interstate Oil and Gas Compact Commission (IOGCC) has been tasked with assisting state governments in the effective, efficient, and environmentally sound regulation of the exploration and production of natural gas and crude oil, specifically in relation to orphaned and abandoned wells and wells nearing the end of productive life. Project goals include: (1) Developing (a) a model framework for prioritization and ranking of orphaned or abandoned well sites; (b) a model framework for disbursement of Energy Policy Act of 2005 funding; and (c) a research study regarding the current status of orphaned wells in the nation. (2) Researching the impact of new technologies on environmental protection from a regulatory perspective. Research will identify and document (a) state reactions to changing technology and knowledge; (b) how those reactions support state environmental conservation and public health; and (c) the impact of those reactions on oil and natural gas production. (3) Assessing emergent technology issues associated with wells nearing the end of productive life. Including: (a) location of orphaned and abandoned well sites; (b) well site remediation; (c) plugging materials; (d) plug placement; (e) the current regulatory environment; and (f) the identification of emergent technologies affecting end of life wells. New Energy Technologies - Regulating Change, is the result of research performed for Tasks 2 and 3.

  3. Office of Technology Transitions

    Broader source: Energy.gov [DOE]

    DOE's Technology Commercialization activities in 2009-13 have involved three broad areas of focus. The primary focus of technology commercialization has continued to be through new technologies developed at the National Laboratories and Facilities. As a second focus, to support and streamline commercialization of these DOE technologies, DOE has carried out a number of new initiatives and pilot projects. Finally, DOE's Department-wide commitment to using commercialization as one mechanism to support U.S. economic growth has led to new cross-cutting programs. U.S. Department of Energy researchers won 31 of the 100 awards in 2014, 36 awards in each of 2013, 2012 and 2011, and 46 in 2010, for a total of 185 over the period of 2009-13. A subset of these awards and other DOE developed technologies are described in Appendix E. These represent a spectrum of commercial areas including DOE mission areas of energy, efficiency, environment and security, as well as spin-off applications in the agricultural, aeronautical, medical, semiconductor and information technology industries, and broad applications in cyber security and sensing/control systems.

  4. Building Technologies Program | Clean Energy | ORNL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Building Technologies Program SHARE Building Technologies Program The Building Technologies Program Office administratively facilitates the integration of ORNL research across...

  5. Materials Technologies: Goals, Strategies, and Top Accomplishments...

    Energy Savers [EERE]

    Materials Technologies: Goals, Strategies, and Top Accomplishments (Brochure), Vehicle Technologies Program (VTP) Materials Technologies: Goals, Strategies, and Top Accomplishments...

  6. Transaction Costs, Information Technology and Development

    E-Print Network [OSTI]

    Singh, Nirvikar

    2004-01-01T23:59:59.000Z

    Transaction Costs, Information Technology and Development 1.Transaction Costs, Information Technology and DevelopmentTransaction Costs, Information Technology and Development *

  7. Transaction Costs, Information Technology and Development

    E-Print Network [OSTI]

    Singh, Nirvikar

    2004-01-01T23:59:59.000Z

    Transaction Costs, Information Technology and Development 1.Transaction Costs, Information Technology and DevelopmentTransaction Costs, Information Technology and Development

  8. ORISE: Training and Technology Support

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Training and Technology Support ORISE helps train all levels of government personnel for natural disasters and man-made emergencies using latest technology The Oak Ridge Institute...

  9. Vehicle Technologies Office: Information Resources

    Broader source: Energy.gov [DOE]

    From here you can access additional information on advanced transportation technologies; view programmatic publications and technical information; learn the basics of hybrid vehicle technology;...

  10. Technology Demonstrations | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    Demonstrations Technology Demonstrations Efficient new building technologies can help meet our country's energy goals, stimulate U.S. manufacturing, create jobs, and improve the...

  11. Technology Investment Roadmap 2012 -2017

    E-Print Network [OSTI]

    Hickman, Mark

    Technology Investment Roadmap 2012 - 2017 20 February 2012 #12;2 Contents Introduction & Overview................................................................... 23 #12;3 Introduction & Overview This Technology Investment Roadmap (TIR) has been developed

  12. Demand response enabling technology development

    E-Print Network [OSTI]

    Arens, Edward; Auslander, David; Huizenga, Charlie

    2008-01-01T23:59:59.000Z

    behavior in developing a demand response future. Phase_II_Demand Response Enabling Technology Development Phase IIYi Yuan The goal of the Demand Response Enabling Technology

  13. Technology Commercialization Showcase - EERE Commercialization...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    the business community. If no one learns of a technology's promise, then that technology will indefinitely sit on the lab shelf. The Solution: The Department Of Energy...

  14. Technology Assistance Program | Partnerships | ORNL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Assistance Program SHARE Technology Assistance Program Electronics Research Assistance is available for small business licensees of ORNL technologies to leverage ORNL's expertise...

  15. Animal Waste Technology Fund (Maryland)

    Broader source: Energy.gov [DOE]

    A bill passed in 2012 transferred responsibility for animal waste management technology projects to the Maryland Department of Agriculture. The Department will maintain the Animal Waste Technology...

  16. Industrial Process Heating - Technology Assessment

    Office of Environmental Management (EM)

    opportunities for technology improvements that can benefit from 146 high-performance computing (HPC) approaches. 147 148 In the next section, the technology assessment...

  17. Next-Generation Photovoltaic Technologies

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Next-Generation Photovoltaic Technologies Next-Generation Photovoltaic Technologies Print Monday, 06 February 2012 15:48 Organic solar cells based on the polymerfullerene bulk...

  18. Fuel Cell Technologies Program Overview

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    CSD Workshop Washington, DC Fuel Cell Technologies Program Overview Dr. Sunita Satyapal Director, Fuel Cell Technologies Office Energy Efficiency and Renewable Energy U.S....

  19. Fuel Cell Technologies Program Overview

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Non-Metallic Materials Meeting Washington, DC Fuel Cell Technologies Program Overview Dr. Sunita Satyapal U.S. Department of Energy Fuel Cell Technologies Program Program Manager...

  20. Video Resources on Geothermal Technologies

    Broader source: Energy.gov [DOE]

    Geothermal video offerings at the Department of Energy include simple interactive illustrations of geothermal power technologies and interviews on initiatives in the Geothermal Technologies Office.

  1. Electric Turbo Compounding Technology Update

    Broader source: Energy.gov (indexed) [DOE]

    Turbo Compounding Technology Update Electric Turbo Compounding Technology Update 15 August, 2007 Carl Vuk 15 August, 2007 Carl Vuk Electric Turbo Compounding Highlights Electric...

  2. Sandia National Laboratories: Vehicle Technologies

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Vehicle Technologies Energy Efficiency On November 11, 2010, in Solid-State Lighting Vehicle Technologies Energy Efficiency News Energy Frontier Research Center for Solid-State...

  3. Immuno-PCR: Very sensitive antigen detection by means of specific antibody-DNA conjugates

    SciTech Connect (OSTI)

    Sano, T.; Smith, C.L.; Cantor, C.R. (Univ. of California, Berkeley (United States))

    1992-10-02T23:59:59.000Z

    An antigen detection system, termed immuno-polymerase chain reaction (immuno-PCR), was developed in which a specific DNA molecule is used as the marker. A streptavidin-protein A chimera that possesses tight and specific binding affinity both for biotin and immunoglobulin G was used to attach a biotinylated DNA specifically to antigen-monoclonal antibody complexes that had been immobilized on microtiter plate wells. Then, a segment of the attached DNA was amplified by PCR. Analysis of the PCR products by agarose gel electrophoresis after staining with ethidium bromide allowed as few as 580 antigen molecules to be readily and reproducibly detected. Direct comparison with enzyme-linked immunosorbent assay with the use of a chimera-alkaline phosphatase conjugate demonstrates that enhancement in detection sensitivity was obtained with the use of immuno-PCR. Given the enormous amplification capability and specificity of PCR, this immuno-PCR technology has a sensitivity greater than any existing antigen detection system and, in principle, could be applied to the detection of single antigen molecules.

  4. Determining orientation and direction of DNA sequences

    DOE Patents [OSTI]

    Goodwin, Edwin H. (Los Alamos, NM); Meyne, Julianne (Los Alamos, NM)

    2000-01-01T23:59:59.000Z

    Determining orientation and direction of DNA sequences. A method by which fluorescence in situ hybridization can be made strand specific is described. Cell cultures are grown in a medium containing a halogenated nucleotide. The analog is partially incorporated in one DNA strand of each chromatid. This substitution takes place in opposite strands of the two sister chromatids. After staining with the fluorescent DNA-binding dye Hoechst 33258, cells are exposed to long-wavelength ultraviolet light which results in numerous strand nicks. These nicks enable the substituted strand to be denatured and solubilized by heat, treatment with high or low pH aqueous solutions, or by immersing the strands in 2.times.SSC (0.3M NaCl+0.03M sodium citrate), to name three procedures. It is unnecessary to enzymatically digest the strands using Exo III or another exonuclease in order to excise and solubilize nucleotides starting at the sites of the nicks. The denaturing/solubilizing process removes most of the substituted strand while leaving the prereplication strand largely intact. Hybridization of a single-stranded probe of a tandem repeat arranged in a head-to-tail orientation will result in hybridization only to the chromatid with the complementary strand present.

  5. NREL: Technology Transfer - Technology Partnership Agreements

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of Science (SC)Integrated CodesTransparency Visit |InfrastructureAerial photoContactsNewsTechnology

  6. NETL Technologies Recognized for Technology Development, Transfer |

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742Energy China 2015of 2005 attheMohammed Khan - TechnologyJanuary 29,guidance on

  7. Technologies Available for Licensing | Partnerships | ORNL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ORNL Technologies Licensing Guidelines NDA(s) and MTA(s) Sample Agreements Technology Innovation Program Technology Assistance Program Licensing Staff Technology Search Engine...

  8. Phylogenetic Analysis of Shewanella Strains by DNA Relatedness Derived from Whole Genome Microarray DNA-DNA Hybridization and Comparison with Other Methods

    SciTech Connect (OSTI)

    Wu, Liyou; Yi, T. Y.; Van Nostrand, Joy; Zhou, Jizhong

    2010-05-17T23:59:59.000Z

    Phylogenetic analyses were done for the Shewanella strains isolated from Baltic Sea (38 strains), US DOE Hanford Uranium bioremediation site [Hanford Reach of the Columbia River (HRCR), 11 strains], Pacific Ocean and Hawaiian sediments (8 strains), and strains from other resources (16 strains) with three out group strains, Rhodopseudomonas palustris, Clostridium cellulolyticum, and Thermoanaerobacter ethanolicus X514, using DNA relatedness derived from WCGA-based DNA-DNA hybridizations, sequence similarities of 16S rRNA gene and gyrB gene, and sequence similarities of 6 loci of Shewanella genome selected from a shared gene list of the Shewanella strains with whole genome sequenced based on the average nucleotide identity of them (ANI). The phylogenetic trees based on 16S rRNA and gyrB gene sequences, and DNA relatedness derived from WCGA hybridizations of the tested Shewanella strains share exactly the same sub-clusters with very few exceptions, in which the strains were basically grouped by species. However, the phylogenetic analysis based on DNA relatedness derived from WCGA hybridizations dramatically increased the differentiation resolution at species and strains level within Shewanella genus. When the tree based on DNA relatedness derived from WCGA hybridizations was compared to the tree based on the combined sequences of the selected functional genes (6 loci), we found that the resolutions of both methods are similar, but the clustering of the tree based on DNA relatedness derived from WMGA hybridizations was clearer. These results indicate that WCGA-based DNA-DNA hybridization is an idea alternative of conventional DNA-DNA hybridization methods and it is superior to the phylogenetics methods based on sequence similarities of single genes. Detailed analysis is being performed for the re-classification of the strains examined.

  9. Ceramic Technology Project

    SciTech Connect (OSTI)

    Not Available

    1992-03-01T23:59:59.000Z

    The Ceramic Technology Project was developed by the USDOE Office of Transportation Systems (OTS) in Conservation and Renewable Energy. This project, part of the OTS's Materials Development Program, was developed to meet the ceramic technology requirements of the OTS's automotive technology programs. Significant accomplishments in fabricating ceramic components for the USDOE and NASA advanced heat engine programs have provided evidence that the operation of ceramic parts in high-temperature engine environments is feasible. These programs have also demonstrated that additional research is needed in materials and processing development, design methodology, and data base and life prediction before industry will have a sufficient technology base from which to produce reliable cost-effective ceramic engine components commercially. A five-year project plan was developed with extensive input from private industry. In July 1990 the original plan was updated through the estimated completion of development in 1993. The objective is to develop the industrial technology base required for reliable ceramics for application in advanced automotive heat engines. The project approach includes determining the mechanisms controlling reliability, improving processes for fabricating existing ceramics, developing new materials with increased reliability, and testing these materials in simulated engine environments to confirm reliability. Although this is a generic materials project, the focus is on the structural ceramics for advanced gas turbine and diesel engines, ceramic bearings and attachments, and ceramic coatings for thermal barrier and wear applications in these engines. To facilitate the rapid transfer of this technology to US industry, the major portion of the work is being done in the ceramic industry, with technological support from government laboratories, other industrial laboratories, and universities.

  10. Technology Available for Licensing Office of Technology Management

    E-Print Network [OSTI]

    Lee, Dongwon

    Technology Available for Licensing Office of Technology Management The Pennsylvania State University 113 Technology Center, University Park, PA 16802 814.865.6277 phone; 814.865.3591 fax Contact: Matthew D. Smith Sr. Technology Licensing Officer The Pennsylvania State University Phone: (814) 863

  11. Information Technology and Management Information Technology and Management

    E-Print Network [OSTI]

    Heller, Barbara

    Information Technology and Management Information Technology and Management IIT School of Applied Technology Daniel F. and Ada L. Rice Campus 201 E. Loop Road Wheaton, IL 60187 www.iit.edu/applied tech/ Dean Technology & Management program is to educate and inform students to prepare them to assume technical

  12. School of Applied Technology School of Applied Technology

    E-Print Network [OSTI]

    Heller, Barbara

    School of Applied Technology School of Applied Technology Daniel F. and Ada L. Rice Campus Illinois Institute of Technology 201 E. Loop Road Wheaton, IL 60187 630.682.6000 www.iit.edu/applied tech/ Dean and Academic Director, Information Technology and Management Programs: C. Robert Carlson Director of Operations

  13. School of Applied Technology School of Applied Technology

    E-Print Network [OSTI]

    Heller, Barbara

    School of Applied Technology School of Applied Technology Daniel F. and Ada L. Rice Campus Illinois Institute of Technology 201 E. Loop Road Wheaton, IL 60187 630.682.6000 www.iit.edu/applied tech/ Dean Technology and Management Programs: Mazin Safar Director, Marketing & Development: Scott Pfeiffer Director

  14. FIELD-BASED TECHNOLOGY EDUCATION: JUST IN TIME TECHNOLOGY TRAINING

    E-Print Network [OSTI]

    Larkin, Teresa L.

    FIELD-BASED TECHNOLOGY EDUCATION: JUST IN TIME TECHNOLOGY TRAINING Sarah Irvine Belson1 and Teresa, Audio Technology, and Physics, American University, 4400 Massachusetts Ave. NW, Washington, DC 20016, tlarkin@american.edu Abstract -- This paper outlines the current status of technology integration

  15. Roadmap: Technology Technology Education Licensure Bachelor of Science

    E-Print Network [OSTI]

    Sheridan, Scott

    Roadmap: Technology ­ Technology Education Licensure ­ Bachelor of Science [AT-BS-TECH-TEDL] College of Applied Engineering, Sustainability and Technology Education Minor [EDUC] College of Education Reasoning TECH 10001 Information Technology 3 TECH 13580 Engineering Graphics 3 C US 10097 Destination

  16. NASA Earth Science Technology Office (ESTO) Decadal Survey Technology Investments

    E-Print Network [OSTI]

    Christian, Eric

    investments · Risks are retired before major dollars are invested: a cost-effective approach to technologyNASA Earth Science Technology Office (ESTO) Decadal Survey Technology Investments January 7, 2009 #12;Overview: Earth Science Technology Office Science Driven, Competed, Actively Managed

  17. Hot Technology, Cool Science (LBNL Science at the Theater)

    ScienceCinema (OSTI)

    None available

    2011-04-28T23:59:59.000Z

    Great innovations start with bold ideas. Learn how Berkeley Lab scientists are devising practical solutions to everything from global warming to how you get to work. On May 11, 2009, five Berkeley Lab scientists participated in a roundtable dicussion ? moderated by KTVU's John Fowler ? on their leading-edge research. This "Science at the Theater" event, held at the Berkeley Repertory Theatre, featured technologies such as cool roofs, battery-driven transportation, a pocket-sized DNA probe, green supercomputing, and a noncontact method for restoring damaged and fragile mechanical recordings.

  18. Probing Minor Groove Hydrogen Bonding Interactions between RB69 DNA Polymerase and DNA

    SciTech Connect (OSTI)

    Xia, Shuangluo; Christian, Thomas D.; Wang, Jimin; Konigsberg, William H. (Yale)

    2012-09-17T23:59:59.000Z

    Minor groove hydrogen bonding (HB) interactions between DNA polymerases (pols) and N3 of purines or O2 of pyrimidines have been proposed to be essential for DNA synthesis from results obtained using various nucleoside analogues lacking the N3 or O2 contacts that interfered with primer extension. Because there has been no direct structural evidence to support this proposal, we decided to evaluate the contribution of minor groove HB interactions with family B pols. We have used RB69 DNA pol and 3-deaza-2'-deoxyadenosine (3DA), an analogue of 2-deoxyadenosine, which has the same HB pattern opposite T but with N3 replaced with a carbon atom. We then determined pre-steady-state kinetic parameters for the insertion of dAMP opposite dT using primer/templates (P/T)-containing 3DA. We also determined three structures of ternary complexes with 3DA at various positions in the duplex DNA substrate. We found that the incorporation efficiency of dAMP opposite dT decreased 10{sup 2}-10{sup 3}-fold even when only one minor groove HB interaction was missing. Our structures show that the HB pattern and base pair geometry of 3DA/dT is exactly the same as those of dA/dT, which makes 3DA an optimal analogue for probing minor groove HB interactions between a DNA polymerase and a nucleobase. In addition, our structures provide a rationale for the observed 10{sup 2}-10{sup 3}-fold decrease in the rate of nucleotide incorporation. The minor groove HB interactions between position n-2 of the primer strand and RB69pol fix the rotomer conformations of the K706 and D621 side chains, as well as the position of metal ion A and its coordinating ligands, so that they are in the optinal orientation for DNA synthesis.

  19. On the feasibility of using the intrinsic fluorescence of nucleotides for DNA sequencing.

    SciTech Connect (OSTI)

    Chowdhury, M. H.; Ray, K.; Johnson, R. L.; Gray, S. K.; Pond, J.; Lakowicz, J. R.; Univ. of Maryland; Univ. of Virginia; Lumerical Solutions, Inc.

    2010-04-29T23:59:59.000Z

    There is presently a worldwide effort to increase the speed and decrease the cost of DNA sequencing as exemplified by the goal of the National Human Genome Research Institute (NHGRI) to sequence a human genome for under $1000. Several high throughput technologies are under development. Among these, single strand sequencing using exonuclease appear very promising. However, this approach requires complete labeling of at least two bases at a time, with extrinsic high quantum yield probes. This is necessary because nucleotides absorb in the deep ultraviolet (UV) and emit with extremely low quantum yields. Hence intrinsic emission from DNA and nucleotides is not being exploited for DNA sequencing. In the present paper we consider the possibility of identifying single nucleotides using their intrinsic emission. We used the finite-difference time-domain (FDTD) method to calculate the effects of aluminum nanoparticles on nearby fluorophores that emit in the UV. We find that the radiated power of UV fluorophores is significantly increased when they are in close proximity to aluminum nanostructures. We show that there will be increased localized excitation near aluminum particles at wavelengths used to excite intrinsic nucleotide emission. Using FDTD simulation we show that a typical DNA base when coupled to appropriate aluminum nanostructures leads to highly directional emission. Additionally we present experimental results showing that a thin film of nucleotides show enhanced emission when in close proximity to aluminum nanostructures. Finally we provide Monte Carlo simulations that predict high levels of base calling accuracy for an assumed number of photons that is derived from the emission spectra of the intrinsic fluorescence of the bases. Our results suggest that single nucleotides can be detected and identified using aluminum nanostructures that enhance their intrinsic emission. This capability would be valuable for the ongoing efforts toward the $1000 genome.

  20. Renewable Energy Resources and Technologies

    Broader source: Energy.gov [DOE]

    Explore the following renewable energy technology areas for resources and information focusing on Federal application opportunities.