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Sample records for dna ormat technologies

  1. Ormat Technologies Inc. Ormat Technologies Reports 2012 Fourth...

    Open Energy Info (EERE)

    Internet. updated 2013;cited 2013. Available from: http:www.ormat.comnewslatest-itemsormat-technologies-reports-2012-fourth-quarter-and-year-end-results Retrieved from...

  2. Ormat Technologies Inc. Ormat Technologies, Inc. Announces a...

    Open Energy Info (EERE)

    Internet. updated 2013;cited 2013. Available from: http:www.ormat.comnewslatest-itemsormat-technologies-inc-announces-non-cash-pre-tax-charge-impairment-its-north-braw...

  3. Josh Nordquist Director Ormat Technologies, Inc. DOE's ...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Converter (OEC) in 1984, at the Wabuska facility outside of Yerington, Nevada, and is still in operation today, proving the reliability and dependability of Ormat's technology. ...

  4. BLM Fact Sheet- Ormat Technologies Salt Wells Geothermal Energy...

    Open Energy Info (EERE)

    Ormat Technologies Salt Wells Geothermal Energy Project Jump to: navigation, search OpenEI Reference LibraryAdd to library Report: BLM Fact Sheet- Ormat Technologies Salt Wells...

  5. Ormat Technologies Inc. North Brawley, California USA | Open...

    Open Energy Info (EERE)

    Not Provided DOI Not Provided Check for DOI availability: http:crossref.org Online Internet link for Ormat Technologies Inc. North Brawley, California USA Citation Ormat...

  6. Glass Buttes Exploration and Drilling: 2010 Geothermal Technologies Program Peer Review Presentation, Walsh, et al, Ormat

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Zemach, Ezra

    Glass Buttes Exploration and Drilling: 2010 Geothermal Technologies Program Peer Review Presentation, Walsh, et al, Ormat

  7. Glass Buttes Exploration and Drilling: 2010 Geothermal Technologies Program Peer Review Presentation, Walsh, et al, Ormat

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Zemach, Ezra

    2010-01-01

    Glass Buttes Exploration and Drilling: 2010 Geothermal Technologies Program Peer Review Presentation, Walsh, et al, Ormat

  8. EA-1944: Ormat Technologies Brady Hot Springs Project, Churchill County, NV

    Broader source: Energy.gov [DOE]

    Ormat Technologies, Inc. (Ormat) proposes to use DOE and cost share funding to study the Brady Hot Springs geothermal Field 15-12RD well. This is an EGS Demonstration project divided into three phases. During Phase 1, Ormat characterized the target well to prepare for stimulation activities in Phase 2, Phase 2: Well Stimulation and Collection/Analysis of Stimulation Monitoring Data and Phase 3: Long-term testing of the system. Phase 2 and 3 activities would occur at Ormat's Brady Hot Springs geothermal field in Churchill County, NV on public lands managed by the Bureau of Land Management (BLM) and Bureau of Reclamation (BOR). Since Phases 2 and 3 have the potential to impact subsurface resources, DOE must analyze the impacts associated with Phases 2 and 3. The BLM will be the lead agency for completion of the EA with BOR and DOE as cooperating agencies.

  9. ORMAT NEVADA | Department of Energy

    Office of Environmental Management (EM)

    It is expected to prevent 301,000 metric tons of carbon dioxide emissions annually. PROJECT STATISTICS: ORMAT NEVADA PROJECT SUMMARY OWNERS Ormat Nevada, Inc. & Ormat ...

  10. LPO5-002-Proj-Poster-CSP-Ormat

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ORMAT NEVADA With 3 facilities across the state, Ormat Nevada increases Nevada's clean power capacity by tapping into a vast underground geothermal reservoir. OWNERS Ormat Nevada, Inc. & Ormat Technologies LOCATIONS Jersey Valley, McGinness Hills & Tuscarora, Nevada LOAN AMOUNT $350 Million ISSUANCE DATE September 2011 GENERATION CAPACITY 97 MW PROJECTED ANNUAL GENERATION 557,000 MWh CLIMATE BENEFIT 301,000 Metric Tons of C0 2 Prevented Annually INVESTING in AMERICAN ENERGY

  11. ORMAT NEVADA | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ORMAT NEVADA ORMAT NEVADA DOE-LPO_Project-Posters_GEO_Ormat-Nevada.pdf (841.29 KB) More Documents & Publications EA-1849: Finding of No Significant Impact EA-1849-S1: Finding of No Significant Impact EA-1849: Final Environmental Assessment

  12. EA-1849: Ormat Nevada Geothermal Projects in Northern NV | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    August 22, 2011 EA-1849: Final Environmental Assessment Tuscarora Geothermal Power Plant, ... Ormat Nevada Northern Nevada Geothermal Power Plant Projects: Loan Guarantee for ORMAT ...

  13. Energy Department Finalizes Loan Guarantee for Ormat Geothermal...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    for Ormat Geothermal Project in Nevada Energy Department Finalizes Loan Guarantee for Ormat Geothermal Project in Nevada September 23, 2011 - 3:37pm Addthis Washington, D.C. - U.S. ...

  14. Energy Department Finalizes Loan Guarantee for Ormat Geothermal Project in

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Nevada | Department of Energy for Ormat Geothermal Project in Nevada Energy Department Finalizes Loan Guarantee for Ormat Geothermal Project in Nevada September 23, 2011 - 3:37pm Addthis Washington, D.C. - U.S. Energy Secretary Steven Chu today announced the Department finalized a partial guarantee for up to a $350 million loan to support a geothermal power generation project. The project, sponsored by Ormat Nevada, Inc., is expected to produce up to 113 megawatts (MW) of clean, baseload

  15. EA-1849: Department of Energy Loan Guarantee to Ormat Nevada, Inc. for a Geothermal Power Facility in Nevada

    Broader source: Energy.gov [DOE]

    Ormat Nevada Inc. (ORMAT), through its subsidiaries, proposes to construct and operate three geothermal power production facilities and associated power transmission lines in northern Nevada. The...

  16. EA-1849-S1: Phase II Facility - Ormat Tuscarora Geothermal Power...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    849-S1: Phase II Facility - Ormat Tuscarora Geothermal Power Plant in Tuscarora, NV EA-1849-S1: Phase II Facility - Ormat Tuscarora Geothermal Power Plant in Tuscarora, NV Summary ...

  17. EA-1849: Ormat Nevada Geothermal Projects in Northern NV | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy 9: Ormat Nevada Geothermal Projects in Northern NV EA-1849: Ormat Nevada Geothermal Projects in Northern NV August 22, 2011 EA-1849: Final Environmental Assessment Tuscarora Geothermal Power Plant, Elko County, Nevada; Jersey Valley Geothermal Project, Pershing County, Nevada; and McGuiness Hills Geothermal Project, Lander County, Nevada August 22, 2011 EA-1849: Finding of No Significant Impact Ormat Nevada Northern Nevada Geothermal Power Plant Projects: Loan Guarantee for ORMAT

  18. EA-1849-S1: Phase II Facility- Ormat Tuscarora Geothermal Power Plant in Tuscarora, NV

    Broader source: Energy.gov [DOE]

    This Supplemental Environmental Assessment (SEA) will evaluate the potential impacts of the Phase II Facility of the Ormat Tuscarora Geothermal Power Plant.

  19. Ormat's North Brawley plant with 17MW short of its 50MW potential...

    Open Energy Info (EERE)

    Site: Ormat's North Brawley plant with 17MW short of its 50MW potential Author Think Geoenergy Published Publisher Not Provided, Date Not Provided DOI Not Provided Check for DOI...

  20. Assembling semiconductor nanocomposites using DNA replication technologies.

    SciTech Connect (OSTI)

    Heimer, Brandon W.; Crown, Kevin K.; Bachand, George David

    2005-11-01

    Deoxyribonucleic acid (DNA) molecules represent Nature's genetic database, encoding the information necessary for all cellular processes. From a materials engineering perspective, DNA represents a nanoscale scaffold with highly refined structure, stability across a wide range of environmental conditions, and the ability to interact with a range of biomolecules. The ability to mass-manufacture functionalized DNA strands with Angstrom-level resolution through DNA replication technology, however, has not been explored. The long-term goal of the work presented in this report is focused on exploiting DNA and in vitro DNA replication processes to mass-manufacture nanocomposite materials. The specific objectives of this project were to: (1) develop methods for replicating DNA strands that incorporate nucleotides with ''chemical handles'', and (2) demonstrate attachment of nanocrystal quantum dots (nQDs) to functionalized DNA strands. Polymerase chain reaction (PCR) and primer extension methodologies were used to successfully synthesize amine-, thiol-, and biotin-functionalized DNA molecules. Significant variability in the efficiency of modified nucleotide incorporation was observed, and attributed to the intrinsic properties of the modified nucleotides. Noncovalent attachment of streptavidin-coated nQDs to biotin-modified DNA synthesized using the primer extension method was observed by epifluorescence microscopy. Data regarding covalent attachment of nQDs to amine- and thiol-functionalized DNA was generally inconclusive; alternative characterization tools are necessary to fully evaluate these attachment methods. Full realization of this technology may facilitate new approaches to manufacturing materials at the nanoscale. In addition, composite nQD-DNA materials may serve as novel recognition elements in sensor devices, or be used as diagnostic tools for forensic analyses. This report summarizes the results obtained over the course of this 1-year project.

  1. Evolutionary theory, web-search technology combine for DNA analysis

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Sequedex: bioinformatics breakthrough Evolutionary theory, web-search technology combine ... Evolutionary theory, web-search technology combine for DNA analysis Bioinformatics ...

  2. Vehicle Technologies Office Merit Review 2014: Fleet DNA

    Broader source: Energy.gov [DOE]

    Presentation given by National Renewable Energy Laboratory at 2014 DOE Hydrogen and Fuel Cells Program and Vehicle Technologies Office Annual Merit Review and Peer Evaluation Meeting about fleet DNA.

  3. Innovative Exploration Technologies Maui Hawaii & Glass Buttes, Oregon |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Technologies Maui Hawaii & Glass Buttes, Oregon Innovative Exploration Technologies Maui Hawaii & Glass Buttes, Oregon Innovative Exploration Technologies Maui Hawii & Glass Buttes, Oregon presentation at the April 2013 peer review meeting held in Denver, Colorado. maui_glass_buttes_ormat_peer2013.pdf (1.01 MB) More Documents & Publications Innovative Exploration Technologies Maui Hawaii & Glass Buttes, Oregon Blind Geothermal System Exploration in

  4. Energy and Technology Review: Unlocking the mysteries of DNA repair

    SciTech Connect (OSTI)

    Quirk, W.A.

    1993-04-01

    DNA, the genetic blueprint, has the remarkable property of encoding its own repair following diverse types of structural damage induced by external agents or normal metabolism. We are studying the interplay of DNA damaging agents, repair genes, and their protein products to decipher the complex biochemical pathways that mediate such repair. Our research focuses on repair processes that correct DNA damage produced by chemical mutagens and radiation, both ionizing and ultraviolet. The most important type of DNA repair in human cells is called excision repair. This multistep process removes damaged or inappropriate pieces of DNA -- often as a string of 29 nucleotides containing the damage -- and replaces them with intact ones. We have isolated, cloned, and mapped several human repair genes associated with the nucleotide excision repair pathway and involved in the repair of DNA damage after exposure to ultraviolet light or mutagens in cooked food. We have shown that a defect in one of these repair genes, ERCC2, is responsible for the repair deficiency in one of the groups of patients with the recessive genetic disorder xeroderma pigmentosum (XP group D). We are exploring ways to purify sufficient quantities (milligrams) of the protein products of these and other repair genes so that we can understand their functions. Our long-term goals are to link defective repair proteins to human DNA repair disorders that predispose to cancer, and to produce DNA-repair-deficient mice that can serve as models for the human disorders.

  5. Innovative Exploration Technologies Maui Hawaii & Glass Buttes, Oregon |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Maui Hawaii & Glass Buttes, Oregon Innovative Exploration Technologies Maui Hawaii & Glass Buttes, Oregon Innovative Exploration Technologies Maui Hawii & Glass Buttes, Oregon presentation at the April 2013 peer review meeting held in Denver, Colorado. moui_glass_buttes_ormat_peer2013.pdf (1.01 MB) More Documents & Publications Innovative Exploration Technologies Maui Hawaii & Glass Buttes, Oregon Blind Geothermal System Exploration in Active Volcanic

  6. Vehicle Technologies Office Merit Review 2013: Fleet DNA

    Broader source: Energy.gov [DOE]

    Presentation given by the National Renewable Energy Laboratory (NREL) at the 2013 DOE Hydrogen and Fuel Cells Program and Vehicle Technologies Program Annual Merit Review and Peer Evaluation Meeting about a tool for analyzing fleet characteristics.

  7. Innovative Exploration Technologies Maui Hawaii & Glass Buttes, Oregon

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Innovative Exploration Technologies Maui Hawaii & Glass Buttes, Oregon Kyle Snyder Ezra Zemach Ormat Nevada Inc. Project Officer: Ava Coy Total Project Funding: Maui-$4.9M, GB- $4.4M April 23rd, 2013 This presentation does not contain any proprietary confidential, or otherwise restricted information. Ulupalakua, Maui Glass Buttes, Oregon 2 | US DOE Geothermal Program eere.energy.gov Maui Overview * Timeline * Project start date 10/29/2009 * Project end date Q3 2013 * Well sites permitting:

  8. Olkaria III Geothermal Power Plant | Open Energy Information

    Open Energy Info (EERE)

    Type Single Flash Owner Ormat Technologies, Inc. Developer Ormat Technologies, Inc. Energy Purchaser Kenya Power and Lighting Company Limited Commercial Online Date 2014...

  9. EA-1944: Final Environmental Assessment | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Ormat Technologies Brady Hot Springs Project, Churchill County, NV Ormat Technologies, ... Brady Hot Springs geothermal field in Churchill County, NV on public lands managed by ...

  10. DNA

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    drives achievement in protein structure research September 15, 2014 Computational analysis key to structural understanding of molecular machine that targets viral DNA LOS ALAMOS, N.M., Sept. 15, 2014-When this week's print issue of the journal Science comes out, a collective cheer will go up from New Mexico, Montana and even the Netherlands, thanks to the type of collaborative effort that is more and more the norm in these connected times. Yes, the research was brilliant, and if we're lucky, it

  11. Doug Hollett, Director Geothermal Technologies Office Geothermal...

    Broader source: Energy.gov (indexed) [DOE]

    ... Ormat (18 MW) Beowawe Power Plant Terra-Gen (1.9 MW) ... Sustaining Maintain optimal subsurface conditions over ... well Productivity Increase flow rates without excessive ...

  12. Bacterial CRISPR/Cas DNA endonucleases: A revolutionary technology that could dramatically impact viral research and treatment

    SciTech Connect (OSTI)

    Kennedy, Edward M.; Cullen, Bryan R.

    2015-05-15

    might be destroyed. In conclusion, we believe that the continued rapid evolution of CRISPR/Cas technology will soon have a major, possibly revolutionary, impact on the field of virology. - Highlights: • Bacterial CRISPR/Cas systems can edit specific DNA sequences in mammalian cells. • CRISPR/Cas systems could eliminate latent or persistent DNA viruses in vivo. • CRISPR/Cas could also be used to screen for viral co-factors or restriction factors.

  13. Vehicle Technologies Office Merit Review 2015: Fleet DNA Phase 1 Refinement & Phase 2 Implementation

    Broader source: Energy.gov [DOE]

    Presentation given by National Renewable Energy Laboratory at 2015 DOE Hydrogen and Fuel Cells Program and Vehicle Technologies Office Annual Merit Review and Peer Evaluation Meeting about Fleet...

  14. Quantitative DNA fiber mapping

    DOE Patents [OSTI]

    Gray, Joe W.; Weier, Heinz-Ulrich G.

    1998-01-01

    The present invention relates generally to the DNA mapping and sequencing technologies. In particular, the present invention provides enhanced methods and compositions for the physical mapping and positional cloning of genomic DNA. The present invention also provides a useful analytical technique to directly map cloned DNA sequences onto individual stretched DNA molecules.

  15. LPO5-002-Proj-Poster-CSP-Solana

    Office of Environmental Management (EM)

    ORMAT NEVADA With 3 facilities across the state, Ormat Nevada increases Nevada's clean power capacity by tapping into a vast underground geothermal reservoir. OWNERS Ormat Nevada, Inc. & Ormat Technologies LOCATIONS Jersey Valley, McGinness Hills & Tuscarora, Nevada LOAN AMOUNT $350 Million ISSUANCE DATE September 2011 GENERATION CAPACITY 97 MW PROJECTED ANNUAL GENERATION 557,000 MWh CLIMATE BENEFIT 301,000 Metric Tons of C0 2 Prevented Annually INVESTING in AMERICAN ENERGY

  16. Technolog

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Research in Science and Technolog y Sandia pushes frontiers of knowledge to meet the nation's needs, today and tomorrow Sandia National Laboratories' fundamental science and technology research leads to greater understanding of how and why things work and is intrinsic to technological advances. Basic research that challenges scientific assumptions enables the nation to push scientific boundaries. Innovations and breakthroughs produced at Sandia allow it to tackle critical issues, from

  17. Technology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Technology Technology Delivering science to the marketplace through commercialization, spinoffs and industry partnerships. News Releases Science Briefs Photos Picture of the Week Publications Social Media Videos Fact Sheets Gary Grider (second from right) with the 2015 Richard P. Feynman Innovation Prize. Also pictured (left to right): Duncan McBranch, Chief Technology Officer of Los Alamos National Laboratory; Terry Wallace, Program Associate Director for Global Security at Los Alamos; and Lee

  18. Technolog

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Research in Science and Technolog y Sandia pushes frontiers of knowledge to meet the nation's needs, today and tomorrow ... Basic research that challenges scientific assumptions ...

  19. Technologies

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The HiWAIS technology is a significant step forward in the warfighter support arena. Honeybees for Explosive Detection Honeybees for Explosive Detection Los Alamos researchers have ...

  20. Chimeric proteins for detection and quantitation of DNA mutations, DNA sequence variations, DNA damage and DNA mismatches

    DOE Patents [OSTI]

    McCutchen-Maloney, Sandra L.

    2002-01-01

    Chimeric proteins having both DNA mutation binding activity and nuclease activity are synthesized by recombinant technology. The proteins are of the general formula A-L-B and B-L-A where A is a peptide having DNA mutation binding activity, L is a linker and B is a peptide having nuclease activity. The chimeric proteins are useful for detection and identification of DNA sequence variations including DNA mutations (including DNA damage and mismatches) by binding to the DNA mutation and cutting the DNA once the DNA mutation is detected.

  1. QER Public Meeting in Pittsburgh, PA: Natural Gas: Transmission...

    Broader source: Energy.gov (indexed) [DOE]

    Midstream Assets and Services, UGI Energy Services - Presentation (3.15 MB) Josh Nordquist, Director of Business Development, Ormat Technologies - Written Statement (144.4 KB) ...

  2. FORGE Teams | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    Ormat Technologies Inc., and Itasca Consulting Group University of Utah Location: Milford City, Utah Key Partners: Utah Geological Survey, Murphy-Brown LLC, Idaho National...

  3. Reno, Nevada: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    Geothermal Technical Partners Gradient Resources Kodali Inc NV Energy Sierra Pacific Resources NV Energy formerly Sierra Pacific Power Newcore Energy Inc Ormat Technologies...

  4. DOI-BLM-NV-CC-ES-11-10-1793 | Open Energy Information

    Open Energy Info (EERE)

    Ormat Technologies, Inc. and Vulcan Power Company are the proponents of the geothermal plants and Sierra Pacific Power Company is the application for the ROW. Combined, the...

  5. EA-1944: Draft Environmental Assessment | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Ormat Technologies Brady Hot Springs Project, Churchill County, NV This EA evaluates a ... Brady Hot Springs geothermal field in Churchill County, NV on public lands managed by ...

  6. NVN-088205 | Open Energy Information

    Open Energy Info (EERE)

    Environmental Analysis Type EA Applicant Ormat Technologies Inc Consultant Environmental Management Associates Geothermal Area Gabbs Valley Geothermal Area Project Location Nevada...

  7. Energy Department Finalizes Loan Guarantee for Ormat Geothermal...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    energy resources, create skilled jobs for American workers and ensure the U.S. remains a global leader in geothermal energy development," said Secretary Chu. "The project...

  8. DOE Meeting Memorandum: Ex Parte Communications

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    September 17, 2014 DOE Attendees: Ashley Armstrong, EERE Outside attendees: Bob Sullivan, Ormat Technologies and Janie Wise, Cassidy & Associates Meeting Contact: Janie Wise - jwise@cassidy.com / 202-585-2553 Subject: Request for Information on Efficiency Standards for Natural Gas Compressors Summary: Ormat Technologies is headquartered in Reno Nevada and designs and manufactures waste heat recovery units that are commonly applied on natural gas pipeline compressor stations. Ormat met with

  9. ORODocumentManagementStstem(HummingbirdDM)PIA.pdf

    Energy Savers [EERE]

    ORMAT NEVADA ORMAT NEVADA DOE-LPO_Project-Posters_GEO_Ormat-Nevada.pdf (841.29 KB) More Documents & Publications EA-1849: Finding of No Significant Impact EA-1849-S1: Finding of No Significant Impact EA-1849: Final Environmental Assessment

    ORMAT NEVADA ORMAT NEVADA ORMAT NEVADA ORMAT NEVADA ORMAT NEVADA ORMAT NEVADA ORMAT NEVADA ORMAT NEVADA ORMAT NEVADA ORMAT NEVADA ORMAT NEVADA PROJECT SUMMARY In September 2011, the Department of Energy issued a $350 million partial loan guarantee under

  10. When DNA Needs to Stand Up and Be Counted

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    When DNA Needs to Stand Up and Be Counted Print DNA microarrays are small metal, glass, or silicon chips covered with patterns of short single-stranded DNA (ssDNA). These "DNA chips" are revolutionizing biotechnology, allowing scientists to identify and count many DNA sequences simultaneously. They are the enabling technology for genomic-based medicine and are a critical component of advanced diagnostic systems for medical and homeland security applications. Like digital chips, DNA

  11. When DNA Needs to Stand Up and Be Counted

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    When DNA Needs to Stand Up and Be Counted Print DNA microarrays are small metal, glass, or silicon chips covered with patterns of short single-stranded DNA (ssDNA). These "DNA chips" are revolutionizing biotechnology, allowing scientists to identify and count many DNA sequences simultaneously. They are the enabling technology for genomic-based medicine and are a critical component of advanced diagnostic systems for medical and homeland security applications. Like digital chips, DNA

  12. When DNA Needs to Stand Up and Be Counted

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    When DNA Needs to Stand Up and Be Counted Print DNA microarrays are small metal, glass, or silicon chips covered with patterns of short single-stranded DNA (ssDNA). These "DNA chips" are revolutionizing biotechnology, allowing scientists to identify and count many DNA sequences simultaneously. They are the enabling technology for genomic-based medicine and are a critical component of advanced diagnostic systems for medical and homeland security applications. Like digital chips, DNA

  13. Dna Sequencing

    DOE Patents [OSTI]

    Tabor, Stanley; Richardson, Charles C.

    1995-04-25

    A method for sequencing a strand of DNA, including the steps off: providing the strand of DNA; annealing the strand with a primer able to hybridize to the strand to give an annealed mixture; incubating the mixture with four deoxyribonucleoside triphosphates, a DNA polymerase, and at least three deoxyribonucleoside triphosphates in different amounts, under conditions in favoring primer extension to form nucleic acid fragments complementory to the DNA to be sequenced; labelling the nucleic and fragments; separating them and determining the position of the deoxyribonucleoside triphosphates by differences in the intensity of the labels, thereby to determine the DNA sequence.

  14. Fleet DNA (Presentation)

    SciTech Connect (OSTI)

    Walkokwicz, K.; Duran, A.

    2014-06-01

    The Fleet DNA project objectives include capturing and quantifying drive cycle and technology variation for the multitude of medium- and heavy-duty vocations; providing a common data storage warehouse for medium- and heavy-duty vehicle fleet data across DOE activities and laboratories; and integrating existing DOE tools, models, and analyses to provide data-driven decision making capabilities. Fleet DNA advantages include: for Government - providing in-use data for standard drive cycle development, R&D, tech targets, and rule making; for OEMs - real-world usage datasets provide concrete examples of customer use profiles; for fleets - vocational datasets help illustrate how to maximize return on technology investments; for Funding Agencies - ways are revealed to optimize the impact of financial incentive offers; and for researchers -a data source is provided for modeling and simulation.

  15. National Account Energy Alliance Final Report for the Basin Electric Project at Northern Border Pipeline Company's Compressor Station #7, North Dakota

    SciTech Connect (OSTI)

    Sweetzer, Richard; Leslie, Neil

    2008-02-01

    A field research test and verification project was conducted at the recovered energy generation plant at Northern Border Pipeline Company Compressor Station #7 (CS#7) near St. Anthony. Recovered energy generation plant equipment was supplied and installed by ORMAT Technologies, Inc. Basin Electric is purchasing the electricity under a purchase power agreement with an ORMAT subsidiary, which owns and operates the plant.

  16. When DNA Needs to Stand Up and Be Counted

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    When DNA Needs to Stand Up and Be Counted When DNA Needs to Stand Up and Be Counted Print Wednesday, 31 May 2006 00:00 DNA microarrays are small metal, glass, or silicon chips covered with patterns of short single-stranded DNA (ssDNA). These "DNA chips" are revolutionizing biotechnology, allowing scientists to identify and count many DNA sequences simultaneously. They are the enabling technology for genomic-based medicine and are a critical component of advanced diagnostic systems for

  17. Fleet DNA Project (Fact Sheet)

    SciTech Connect (OSTI)

    Not Available

    2012-10-01

    The Fleet DNA Project - designed by the U.S. Department of Energy's National Renewable Energy Laboratory (NREL) in partnership with Oak Ridge National Laboratory - aims to accelerate the evolution of advanced vehicle development and support the strategic deployment of market-ready technologies that reduce costs, fuel consumption, and emissions. At the heart of the Fleet DNA Project is a clearinghouse of medium- and heavy-duty commercial fleet transportation data for optimizing the design of advanced vehicle technologies or for selecting a given technology to invest in. An easy-to-access online database will help vehicle manufacturers and fleets understand the broad operational range for many of today's commercial vehicle vocations.

  18. Recent advances in yeast molecular biology: recombinant DNA. [Lead abstract

    SciTech Connect (OSTI)

    Not Available

    1982-09-01

    Separate abstracts were prepared for the 25 papers presented at a workshop focusing on chromosomal structure, gene regulation, recombination, DNA repair, and cell type control, that have been obtained by experimental approaches incorporating the new technologies of yeast DNA transformation, molecular cloning, and DNA sequence analysis. (KRM)

  19. Storing data encoded DNA in living organisms

    DOE Patents [OSTI]

    Wong; Pak C. , Wong; Kwong K. , Foote; Harlan P.

    2006-06-06

    Current technologies allow the generation of artificial DNA molecules and/or the ability to alter the DNA sequences of existing DNA molecules. With a careful coding scheme and arrangement, it is possible to encode important information as an artificial DNA strand and store it in a living host safely and permanently. This inventive technology can be used to identify origins and protect R&D investments. It can also be used in environmental research to track generations of organisms and observe the ecological impact of pollutants. Today, there are microorganisms that can survive under extreme conditions. As well, it is advantageous to consider multicellular organisms as hosts for stored information. These living organisms can provide as memory housing and protection for stored data or information. The present invention provides well for data storage in a living organism wherein at least one DNA sequence is encoded to represent data and incorporated into a living organism.

  20. DNA polymerase with modified processivity

    DOE Patents [OSTI]

    Bedford, Ella; Tabor, Stanley; Richardson, Charles C.

    1999-01-01

    Chimeric DNA polymerase having a DNA polymerase domain and processivity factor binding domain not naturally associated with DNA polymerase domain.

  1. Rapid Detection and Identification of a Pathogen's DNA Using Phi29 DNA Polymerase

    SciTech Connect (OSTI)

    Xu, Y.; Dunn, J.; Gao, S.; Bruno, J. F.; Luft, B. J.

    2008-10-31

    Zoonotic pathogens including those transmitted by insect vectors are some of the most deadly of all infectious diseases known to mankind. A number of these agents have been further weaponized and are widely recognized as being potentially significant biothreat agents. We describe a novel method based on multiply-primed rolling circle in vitro amplification for profiling genomic DNAs to permit rapid, cultivation-free differential detection and identification of circular plasmids in infectious agents. Using Phi29 DNA polymerase and a two-step priming reaction we could reproducibly detect and characterize by DNA sequencing circular DNA from Borrelia burgdorferi B31 in DNA samples containing as little as 25 pg of Borrelia DNA amongst a vast excess of human DNA. This simple technology can ultimately be adapted as a sensitive method to detect specific DNA from both known and unknown pathogens in a wide variety of complex environments.

  2. Synthesis of DNA

    DOE Patents [OSTI]

    Mariella, Jr., Raymond P.

    2008-11-18

    A method of synthesizing a desired double-stranded DNA of a predetermined length and of a predetermined sequence. Preselected sequence segments that will complete the desired double-stranded DNA are determined. Preselected segment sequences of DNA that will be used to complete the desired double-stranded DNA are provided. The preselected segment sequences of DNA are assembled to produce the desired double-stranded DNA.

  3. DNA encoding a DNA repair protein

    DOE Patents [OSTI]

    Petrini, John H.; Morgan, William Francis; Maser, Richard Scott; Carney, James Patrick

    2006-08-15

    An isolated and purified DNA molecule encoding a DNA repair protein, p95, is provided, as is isolated and purified p95. Also provided are methods of detecting p95 and DNA encoding p95. The invention further provides p95 knock-out mice.

  4. CX-006662: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Geothermal Technologies Program - ORMAT GeneratorCX(s) Applied: B3.6, B5.1Date: 10/26/2009Location(s): Casper, WyomingOffice(s): RMOTC

  5. Geothermal EGS Demonstration Photo Library | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Geothermal EGS Demonstration Photo Library Geothermal EGS Demonstration Photo Library Addthis Energy Department EGS Demonstration Yields First Commercial Power to the Grid 1 of 4 Energy Department EGS Demonstration Yields First Commercial Power to the Grid In April 2013, DOE industry partner Ormat Technologies succeeded in generating the nation's first commercial power from enhanced geothermal systems to the grid at Desert Peak, Nevada. Image: courtesy Ormat Technologies AltaRock

  6. Binary electrokinetic separation of target DNA from background DNA primers.

    SciTech Connect (OSTI)

    James, Conrad D.; Derzon, Mark Steven

    2005-10-01

    This report contains the summary of LDRD project 91312, titled ''Binary Electrokinetic Separation of Target DNA from Background DNA Primers''. This work is the first product of a collaboration with Columbia University and the Northeast BioDefense Center of Excellence. In conjunction with Ian Lipkin's lab, we are developing a technique to reduce false positive events, due to the detection of unhybridized reporter molecules, in a sensitive and multiplexed detection scheme for nucleic acids developed by the Lipkin lab. This is the most significant problem in the operation of their capability. As they are developing the tools for rapidly detecting the entire panel of hemorrhagic fevers this technology will immediately serve an important national need. The goal of this work was to attempt to separate nucleic acid from a preprocessed sample. We demonstrated the preconcentration of kilobase-pair length double-stranded DNA targets, and observed little preconcentration of 60 base-pair length single-stranded DNA probes. These objectives were accomplished in microdevice formats that are compatible with larger detection systems for sample pre-processing. Combined with Columbia's expertise, this technology would enable a unique, fast, and potentially compact method for detecting/identifying genetically-modified organisms and multiplexed rapid nucleic acid identification. Another competing approach is the DARPA funded IRIS Pharmaceutical TIGER platform which requires many hours for operation, and an 800k$ piece of equipment that fills a room. The Columbia/SNL system could provide a result in 30 minutes, at the cost of a few thousand dollars for the platform, and would be the size of a shoebox or smaller.

  7. DNA tagged microparticles

    DOE Patents [OSTI]

    Farquar, George Roy; Leif, Roald N; Wheeler, Elizabeth

    2015-05-05

    A simulant that includes a carrier and DNA encapsulated in the carrier. Also a method of making a simulant including the steps of providing a carrier and encapsulating DNA in the carrier to produce the simulant.

  8. NREL: Transportation Research - Fleet DNA: Commercial Fleet Vehicle

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Operating Data Fleet DNA: Commercial Fleet Vehicle Operating Data Contribute Data Learn how to contribute to Fleet DNA anonymously to help other fleets analyze and improve their drive cycle metrics. The Fleet DNA clearinghouse of commercial fleet vehicle operating data helps vehicle manufacturers and developers optimize vehicle designs and helps fleet managers choose advanced technologies for their fleets. This online tool provides data summaries and visualizations similar to real-world

  9. Vehicle Technologies Office: Technologies

    Office of Energy Efficiency and Renewable Energy (EERE)

    To support DOE's goal to provide clean and secure energy, the Vehicle Technologies Office (VTO) invests in research and development that:

  10. DNA Sequencing apparatus

    DOE Patents [OSTI]

    Tabor, Stanley; Richardson, Charles C.

    1992-01-01

    An automated DNA sequencing apparatus having a reactor for providing at least two series of DNA products formed from a single primer and a DNA strand, each DNA product of a series differing in molecular weight and having a chain terminating agent at one end; separating means for separating the DNA products to form a series bands, the intensity of substantially all nearby bands in a different series being different, band reading means for determining the position an This invention was made with government support including a grant from the U.S. Public Health Service, contract number AI-06045. The U.S. government has certain rights in the invention.

  11. Requirements Flowdown and Graded Approach to QA

    Office of Environmental Management (EM)

    | Department of Energy Efficiency Standards for Natural Gas Compressors Request for Information on Efficiency Standards for Natural Gas Compressors Ormat Technologies is headquartered in Reno Nevada and designs and manufactures waste heat recovery units that are commonly applied on natural gas pipeline compressor stations Ormat EERE meeting memo (31.06 KB) More Documents & Publications DOE Meeting Memorandum: Ex Parte Communications Geothermal Energy Production with Co-produced and

  12. Request for Information on Efficiency Standards for Natural Gas Compressors

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    | Department of Energy Efficiency Standards for Natural Gas Compressors Request for Information on Efficiency Standards for Natural Gas Compressors Ormat Technologies is headquartered in Reno Nevada and designs and manufactures waste heat recovery units that are commonly applied on natural gas pipeline compressor stations Ormat EERE meeting memo (31.06 KB) More Documents & Publications DOE Meeting Memorandum: Ex Parte Communications Geothermal Energy Production with Co-produced and

  13. ORNI 9, LLC, AND ORMAT NEVADA INC. APPLICATIONS FOR PERMIT TO...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    All onsite drilling materials and chemicals shall be properly stored to ensure the ... andor noxious fluids are spilled during drilling, testing, and completion operations, ...

  14. Detection and quantitation of single nucleotide polymorphisms, DNA sequence variations, DNA mutations, DNA damage and DNA mismatches

    DOE Patents [OSTI]

    McCutchen-Maloney, Sandra L.

    2002-01-01

    DNA mutation binding proteins alone and as chimeric proteins with nucleases are used with solid supports to detect DNA sequence variations, DNA mutations and single nucleotide polymorphisms. The solid supports may be flow cytometry beads, DNA chips, glass slides or DNA dips sticks. DNA molecules are coupled to solid supports to form DNA-support complexes. Labeled DNA is used with unlabeled DNA mutation binding proteins such at TthMutS to detect DNA sequence variations, DNA mutations and single nucleotide length polymorphisms by binding which gives an increase in signal. Unlabeled DNA is utilized with labeled chimeras to detect DNA sequence variations, DNA mutations and single nucleotide length polymorphisms by nuclease activity of the chimera which gives a decrease in signal.

  15. NREL: Technology Deployment - Technology Acceleration

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Technology Acceleration NREL offers technology-specific assistance to federal and private industry to help address market barriers to sustainable energy technologies. Learn more ...

  16. Screening Tool for Providers of Double-Stranded DNA - Energy Innovation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Portal Startup America Startup America Industrial Technologies Industrial Technologies Energy Analysis Energy Analysis Biomass and Biofuels Biomass and Biofuels Find More Like This Return to Search Screening Tool for Providers of Double-Stranded DNA Oak Ridge National Laboratory Contact ORNL About This Technology Publications: PDF Document Publication 11-G00227_ID2510 (2).pdf (829 KB) Technology Marketing SummaryThe invention screens purchase orders submitted to DNA synthesis

  17. DNA ELECTROPHORESIS AT SURFACES

    SciTech Connect (OSTI)

    RAFAILOVICH, MIRIAM; SOKOLOV, JONATHAN; GERSAPPE, DILIP

    2003-09-01

    During this year we performed two major projects: I. We developed a detailed theoretical model which complements our experiments on surface DNA electrophoresis. We found that it was possible to enhance the separation of DNA chains by imposing a chemical nanoscale pattern on the surface. This approach utilized the surface interaction effect of the DNA chains with the substrate and is a refinement to our previous method in which DNA chains were separated on homogeneous flat surfaces. By introducing the nano-patterns on the surface, the conformational changes of DNA chains of different lengths can be amplified, which results in the different friction strengths with the substrate surface. Our results also show that, when compared to the DNA electrophoresis performed on homogeneous flat surfaces, nanopatterned surfaces offer a larger window in choosing different surface interactions to achieve separation. II. In collaboration with a large international manufacturer of skin care products we also embarked on a project involving photo toxicity of titanium dioxide nanoparticles, which are a key ingredient in sunscreen and cosmetic lotions. The results clearly implicated the nanoparticles in catalyzing damage to chromosomal DNA. We then used this knowledge to develop a polymer/anti-oxidant coating which prevented the photocatalytic reaction on DNA while still retaining the UV absorptive properties of the nanoparticles. The standard gel electrophoresis was not sufficient in determining the extent of the DNA damage. The conclusions of this study were based predominantly on analysis obtained with the surface electrophoresis method.

  18. Controlling DNA Methylation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Controlling DNA Methylation Though life on earth is composed of a diverse range of organisms, some with many different types of tissues and cells, all these are encoded by a molecule we call DNA. The information required to build a protein is stored in DNA within the cells. Not all the message in the DNA is used in each cell and not all the message is used all the time. During cell differentiation, the cells become dedicated for their specific function which involves selectively activating some

  19. Don A. Cambell Geothermal Power Plant | Open Energy Information

    Open Energy Info (EERE)

    Geothermal Region Plant Information Facility Type Binary Owner Ormat Developer Ormat Energy Purchaser Ormat Commercial Online Date 2013 Power Plant Data Type of Plant Number...

  20. Map of Geothermal Facilities/Data | Open Energy Information

    Open Energy Info (EERE)

    TW 1 1988 Don A. Cambell Geothermal Power Plant Binary Ormat Ormat Ormat 2013 Dora-1 Geothermal Energy Power Plant Binary Cycle Power Plant, ORC Menderes Geothermal Menderes...

  1. Installed Geothermal Capacity/Data | Open Energy Information

    Open Energy Info (EERE)

    TW 1 1988 Don A. Cambell Geothermal Power Plant Binary Ormat Ormat Ormat 2013 Dora-1 Geothermal Energy Power Plant Binary Cycle Power Plant, ORC Menderes Geothermal Menderes...

  2. DNA-cell conjugates

    DOE Patents [OSTI]

    Hsiao, Shih-Chia; Francis, Matthew B.; Bertozzi, Carolyn; Mathies, Richard; Chandra, Ravi; Douglas, Erik; Twite, Amy; Toriello, Nicholas; Onoe, Hiroaki

    2016-05-03

    The present invention provides conjugates of DNA and cells by linking the DNA to a native functional group on the cell surface. The cells can be without cell walls or can have cell walls. The modified cells can be linked to a substrate surface and used in assay or bioreactors.

  3. Quantitive DNA Fiber Mapping

    SciTech Connect (OSTI)

    Lu, Chun-Mei; Wang, Mei; Greulich-Bode, Karin M.; Weier, Jingly F.; Weier, Heinz-Ulli G.

    2008-01-28

    Several hybridization-based methods used to delineate single copy or repeated DNA sequences in larger genomic intervals take advantage of the increased resolution and sensitivity of free chromatin, i.e., chromatin released from interphase cell nuclei. Quantitative DNA fiber mapping (QDFM) differs from the majority of these methods in that it applies FISH to purified, clonal DNA molecules which have been bound with at least one end to a solid substrate. The DNA molecules are then stretched by the action of a receding meniscus at the water-air interface resulting in DNA molecules stretched homogeneously to about 2.3 kb/{micro}m. When non-isotopically, multicolor-labeled probes are hybridized to these stretched DNA fibers, their respective binding sites are visualized in the fluorescence microscope, their relative distance can be measured and converted into kilobase pairs (kb). The QDFM technique has found useful applications ranging from the detection and delineation of deletions or overlap between linked clones to the construction of high-resolution physical maps to studies of stalled DNA replication and transcription.

  4. DNA-PK assay

    DOE Patents [OSTI]

    Anderson, Carl W.; Connelly, Margery A.

    2004-10-12

    The present invention provides a method for detecting DNA-activated protein kinase (DNA-PK) activity in a biological sample. The method includes contacting a biological sample with a detectably-labeled phosphate donor and a synthetic peptide substrate defined by the following features to provide specific recognition and phosphorylation by DNA-PK: (1) a phosphate-accepting amino acid pair which may include serine-glutamine (Ser-Gln) (SQ), threonine-glutamine (Thr-Gln) (TQ), glutamine-serine (Gln-Ser) (QS), or glutamine-threonine (Gln-Thr) (QT); (2) enhancer amino acids which may include glutamic acid or glutamine immediately adjacent at the amino- or carboxyl- side of the amino acid pair and forming an amino acid pair-enhancer unit; (3) a first spacer sequence at the amino terminus of the amino acid pair-enhancer unit; (4) a second spacer sequence at the carboxyl terminus of the amino acid pair-enhancer unit, which spacer sequences may include any combination of amino acids that does not provide a phosphorylation site consensus sequence motif; and, (5) a tag moiety, which may be an amino acid sequence or another chemical entity that permits separating the synthetic peptide from the phosphate donor. A compostion and a kit for the detection of DNA-PK activity are also provided. Methods for detecting DNA, protein phosphatases and substances that alter the activity of DNA-PK are also provided. The present invention also provides a method of monitoring protein kinase and DNA-PK activity in living cells. -A composition and a kit for monitoring protein kinase activity in vitro and a composition and a kit for monitoring DNA-PK activities in living cells are also provided. A method for identifying agents that alter protein kinase activity in vitro and a method for identifying agents that alter DNA-PK activity in living cells are also provided.

  5. Energy Technologies

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Our Vision National User Facilities Research Areas In Focus Global Solutions Energy Technologies Area (ETA) Building Technology & Urban Systems Energy Analysis & Environmental...

  6. Enhancing the DNA Patent Database

    SciTech Connect (OSTI)

    Walters, LeRoy B.

    2008-02-18

    Final Report on Award No. DE-FG0201ER63171 Principal Investigator: LeRoy B. Walters February 18, 2008 This project successfully completed its goal of surveying and reporting on the DNA patenting and licensing policies at 30 major U.S. academic institutions. The report of survey results was published in the January 2006 issue of Nature Biotechnology under the title The Licensing of DNA Patents by US Academic Institutions: An Empirical Survey. Lori Pressman was the lead author on this feature article. A PDF reprint of the article will be submitted to our Program Officer under separate cover. The project team has continued to update the DNA Patent Database on a weekly basis since the conclusion of the project. The database can be accessed at dnapatents.georgetown.edu. This database provides a valuable research tool for academic researchers, policymakers, and citizens. A report entitled Reaping the Benefits of Genomic and Proteomic Research: Intellectual Property Rights, Innovation, and Public Health was published in 2006 by the Committee on Intellectual Property Rights in Genomic and Protein Research and Innovation, Board on Science, Technology, and Economic Policy at the National Academies. The report was edited by Stephen A. Merrill and Anne-Marie Mazza. This report employed and then adapted the methodology developed by our research project and quoted our findings at several points. (The full report can be viewed online at the following URL: http://www.nap.edu/openbook.php?record_id=11487&page=R1). My colleagues and I are grateful for the research support of the ELSI program at the U.S. Department of Energy.

  7. DNA | Open Energy Information

    Open Energy Info (EERE)

    Lead Agency District Office Development Phase(s) Techniques DNA-NV-030-09-03 Dusty Miller LLC BLM BLM Carson City District Office BLM Stillwater Field Office BLM...

  8. Multiplex analysis of DNA

    DOE Patents [OSTI]

    Church, George M.; Kieffer-Higgins, Stephen

    1992-01-01

    This invention features vectors and a method for sequencing DNA. The method includes the steps of: a) ligating the DNA into a vector comprising a tag sequence, the tag sequence includes at least 15 bases, wherein the tag sequence will not hybridize to the DNA under stringent hybridization conditions and is unique in the vector, to form a hybrid vector, b) treating the hybrid vector in a plurality of vessels to produce fragments comprising the tag sequence, wherein the fragments differ in length and terminate at a fixed known base or bases, wherein the fixed known base or bases differs in each vessel, c) separating the fragments from each vessel according to their size, d) hybridizing the fragments with an oligonucleotide able to hybridize specifically with the tag sequence, and e) detecting the pattern of hybridization of the tag sequence, wherein the pattern reflects the nucleotide sequence of the DNA.

  9. Patterning nanocrystals using DNA

    SciTech Connect (OSTI)

    Williams, Shara Carol

    2003-09-01

    One of the goals of nanotechnology is to enable programmed self-assembly of patterns made of various materials with nanometer-sized control. This dissertation describes the results of experiments templating arrangements of gold and semiconductor nanocrystals using 2'-deoxyribonucleic acid (DNA). Previously, simple DNA-templated linear arrangements of two and three nanocrystals structures have been made.[1] Here, we have sought to assemble larger and more complex nanostructures. Gold-DNA conjugates with 50 to 100 bases self-assembled into planned arrangements using strands of DNA containing complementary base sequences. We used two methods to increase the complexity of the arrangements: using branched synthetic doublers within the DNA covalent backbone to create discrete nanocrystal groupings, and incorporating the nanocrystals into a previously developed DNA lattice structure [2][3] that self-assembles from tiles made of DNA double-crossover molecules to create ordered nanoparticle arrays. In the first project, the introduction of a covalently-branched synthetic doubler reagent into the backbone of DNA strands created a branched DNA ''trimer.'' This DNA trimer templated various structures that contained groupings of three and four gold nanoparticles, giving promising, but inconclusive transmission electron microscopy (TEM) results. Due to the presence of a variety of possible structures in the reaction mixtures, and due to the difficulty of isolating the desired structures, the TEM and gel electrophoresis results for larger structures having four particles, and for structures containing both 5 and 10 nm gold nanoparticles were inconclusive. Better results may come from using optical detection methods, or from improved sample preparation. In the second project, we worked toward making two-dimensional ordered arrays of nanocrystals. We replicated and improved upon previous results for making DNA lattices, increasing the size of the lattices to a length greater than

  10. Exploration Technologies Technology Needs Assessment

    Broader source: Energy.gov [DOE]

    The Exploration Technologies Needs Assessment is a critical component of ongoing technology roadmapping efforts, and will be used to guide the program's research and development.

  11. Exploration Technologies - Technology Needs Assessment

    SciTech Connect (OSTI)

    Greene, Amanda I.; Thorsteinsson, Hildigunnur; Reinhardt, Tim; Solomon, Samantha; James, Mallory

    2011-06-01

    This assessment is a critical component of ongoing technology roadmapping efforts, and will be used to guide the Geothermal Technology Program's research and development.

  12. High Impact Technology Catalyst: Technology Deployment Strategies...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Catalyst: Technology Deployment Strategies High Impact Technology Catalyst: Technology Deployment Strategies The Energy Department released the High Impact Technology Catalyst: ...

  13. NREL: Technology Transfer - Technology Partnership Agreements

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ombuds. Printable Version Technology Transfer Home About Technology Transfer Technology Partnership Agreements Agreements for Commercializing Technology CRADAs Work for...

  14. NREL: Technology Transfer - Technologies Available for Licensing

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ombuds. Printable Version Technology Transfer Home About Technology Transfer Technology Partnership Agreements Licensing Agreements Technologies Available for Licensing...

  15. Vehicle Technologies Office: Graduate Automotive Technology Education...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Education & Workforce Development Vehicle Technologies Office: Graduate Automotive Technology Education (GATE) Vehicle Technologies Office: Graduate Automotive Technology ...

  16. Vehicle Technologies Office - Materials Technologies

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Vehicle Technologies Office Materials Technologies Ed Owens Jerry Gibbs Will Joost eere.energy.gov 2 | Vehicle Technologies Program Materials Technologies Materials Technologies $36.9 M Lightweight Materials $28.0 M Values are FY14 enacted Propulsion Materials $8.9 M Properties and Manufacturing Multi-Material Enabling Modeling & Computational Mat. Sci. Engine Materials, Cast Al & Fe High Temp Alloys Exhaust Sys. Materials, Low T Catalysts Lightweight Propulsion FY13 Enacted $27.5 M

  17. Thermally Activated Technologies Technology Roadmap, May 2003...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Thermally Activated Technologies Technology Roadmap, May 2003 Thermally Activated Technologies Technology Roadmap, May 2003 The purpose of this Technology Roadmap is to outline a ...

  18. Available Technologies

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    application. Search Our Technologies submit Advanced Materials Advanced Materials Biotechnology Biotechnology Chemistry Chemistry Energy Energy High Performance Computing:...

  19. Licensing Technology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Licensing Technology Licensing Technology The primary function of Los Alamos Licensing Program is to move Los Alamos technology to the marketplace for the benefit of the U.S. economy. Our intellectual property may be licensed for commercial use, research applications, and U.S. government use. Contact thumbnail of Marcus Lucero Head of Licensing Marcus Lucero Richard P. Feynman Center for Innovation (505) 665-6569 Email Although Los Alamos's primary mission is national security, our technologies

  20. Technology Opportunities

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Intellectual Property » Technology Opportunities Technology Opportunities We deliver innovation through an integrated portfolio of R&D work across our key national security sponsoring agencies, enhanced by the ideas developed through our strategic internal investments. Contact Business Development Team Richard P. Feynman Center for Innovation (505) 665-9090 Email Periodically, the Laboratory notifies the public of technologies and capabilities that may be of interest. These technologies may

  1. Technology Assessment

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    - FOR OFFICIAL USE ONLY - DRAFT 1 Advanced Composites Materials and their Manufacture 1 Technology Assessment 2 Contents 3 1. Introduction to the Technology/System ................................................................................................ 2 4 2. Technology Potential and Assessment .................................................................................................. 4 5 2.1 The Potential for Advanced Composites for Clean Energy Application Areas

  2. DNA polymerase having modified nucleotide binding site for DNA sequencing

    DOE Patents [OSTI]

    Tabor, Stanley; Richardson, Charles

    1997-01-01

    Modified gene encoding a modified DNA polymerase wherein the modified polymerase incorporates dideoxynucleotides at least 20-fold better compared to the corresponding deoxynucleotides as compared with the corresponding naturally-occurring DNA polymerase.

  3. DNA polymerase having modified nucleotide binding site for DNA sequencing

    DOE Patents [OSTI]

    Tabor, S.; Richardson, C.

    1997-03-25

    A modified gene encoding a modified DNA polymerase is disclosed. The modified polymerase incorporates dideoxynucleotides at least 20-fold better compared to the corresponding deoxynucleotides as compared with the corresponding naturally-occurring DNA polymerase. 6 figs.

  4. DNA tagged microparticles

    DOE Patents [OSTI]

    Farquar, George R.; Leif, Roald N.; Wheeler, Elizabeth

    2016-03-22

    In one embodiment, a product includes a plurality of particles, each particle including: a carrier that includes a non-toxic material; and at least one DNA barcode coupled to the carrier, where the particles each have a diameter in a range from about 1 nanometer to about 100 microns.

  5. Contributing Data to the Fleet DNA Project (Brochure), NREL (National Renewable Energy Laboratory)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Photo from iStock/9420677 Contributing Data to the Fleet DNA Project Sponsored by the U.S. Department of Energy, the Fleet DNA project aims to accelerate the evolution of advanced vehicle development and support the strategic deployment of market-ready technologies that reduce costs, fuel consumption, and emissions. The Fleet DNA clearinghouse of commercial fleet transportation data helps vehicle manufacturers and developers optimize vehicle designs and helps fleet managers choose advanced

  6. Technology '90

    SciTech Connect (OSTI)

    Not Available

    1991-01-01

    The US Department of Energy (DOE) laboratories have a long history of excellence in performing research and development in a number of areas, including the basic sciences, applied-energy technology, and weapons-related technology. Although technology transfer has always been an element of DOE and laboratory activities, it has received increasing emphasis in recent years as US industrial competitiveness has eroded and efforts have increased to better utilize the research and development resources the laboratories provide. This document, Technology '90, is the latest in a series that is intended to communicate some of the many opportunities available for US industry and universities to work with the DOE and its laboratories in the vital activity of improving technology transfer to meet national needs. Technology '90 is divided into three sections: Overview, Technologies, and Laboratories. The Overview section describes the activities and accomplishments of the DOE research and development program offices. The Technologies section provides descriptions of new technologies developed at the DOE laboratories. The Laboratories section presents information on the missions, programs, and facilities of each laboratory, along with a name and telephone number of a technology transfer contact for additional information. Separate papers were prepared for appropriate sections of this report.

  7. Technology Partnering

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Transfer and Related Technology Partnering Activities at the National Laboratories and Other Facilities Fiscal Years 2009-2013 Report to Congress May 2015 United States Department of Energy Washington, DC 20585 Message from the Secretary The Report on Technology Transfer and Related Partnering Activities at the National Laboratories and Other Facilities for Fiscal Year 2009-2013 is prepared in accordance with the requirements of the Technology Transfer and Commercialization Act of 2000: It is

  8. Technology Transfer

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    technology transfer Technology Transfer Since 1974, the Federal Laboratory Consortium (FLC) Award for Excellence in Technology Transfer has recognized scientists and engineers at federal government and research centers for their "uncommon creativity and initiative in conveying innovations from their facilities to industry and local government." Scientists and engineers from more than 650 federal government laboratories and research centers compete for the 30 awards presented each year.

  9. Fuels Technologies

    Office of Environmental Management (EM)

    Displacement of petroleum n Approach n Example Project Accomplishments n Research Directions Fuels Technologies R&D Budget by Activities Major Activities FY 2007 ...

  10. NREL: Technology Transfer - Agreements for Commercializing Technology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    303-384-7353. Printable Version Technology Transfer Home About Technology Transfer Technology Partnership Agreements Agreements for Commercializing Technology CRADAs Work for...

  11. Huazhong Science Technology University Yongtai Science Technology...

    Open Energy Info (EERE)

    Huazhong Science Technology University Yongtai Science Technology Co Ltd Jump to: navigation, search Name: Huazhong Science & Technology University Yongtai Science & Technology Co...

  12. Hardware Controller DNA Synthesizer

    Energy Science and Technology Software Center (OSTI)

    1995-07-27

    The program controls the operation of various hardware components of an automatic 12-channel parrallel oligosynthesizer. This involves accepting information regarding the DNA sequence to be generated and converting this into a series of instructions to I/O ports to actuate the appropriate hardware components. The design and function of the software is specific to a particular hardware platform and has no utility for controlling other configurations.

  13. Identification of Human Repetitive DNA Elements

    Energy Science and Technology Software Center (OSTI)

    1995-11-01

    PYTHIA identifies the subfamily membership of Alu sequences, occurrences of repetitive human DNA elements, and simple DNA sequences.

  14. Thermally activated technologies: Technology Roadmap

    SciTech Connect (OSTI)

    None, None

    2003-05-01

    The purpose of this Technology Roadmap is to outline a set of actions for government and industry to develop thermally activated technologies for converting America’s wasted heat resources into a reservoir of pollution-free energy for electric power, heating, cooling, refrigeration, and humidity control. Fuel flexibility is important. The actions also cover thermally activated technologies that use fossil fuels, biomass, and ultimately hydrogen, along with waste heat.

  15. Technology Assessment

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Roll to Roll (R2R) Processing 1 Technology Assessment 2 3 Contents 4 1. Introduction to the Technology/System ............................................................................................... 2 5 1.1. Introduction to R2R Processing..................................................................................................... 2 6 1.2. R2R Processing Mechanisms ......................................................................................................... 3 7 2.

  16. Technology Commercialization Showcase 2008 Vehicle Technologies Program

    SciTech Connect (OSTI)

    Davis, Patrick B.

    2009-06-19

    Presentation illustrating various technology commercialization opportunities and unexploited investment gaps for the Vehicle Technologies Program.

  17. Universal Gene Transfer Technology for Gram Positive Bacteria - Energy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Innovation Portal Biomass and Biofuels Biomass and Biofuels Advanced Materials Advanced Materials Find More Like This Return to Search Universal Gene Transfer Technology for Gram Positive Bacteria Oak Ridge National Laboratory Contact ORNL About This Technology Publications: PDF Document Publication 11-G00255_ID2139_rev.pdf (493 KB) Technology Marketing SummaryA genetic engineering technology invented at ORNL facilitates DNA delivery to a cell by using ultrasound to permeate the cell's

  18. Tag: technology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Tags

    technology<...

  19. Technology Validation

    Broader source: Energy.gov [DOE]

    To reduce solar technology risks, DOE and its partners evaluate the performance and reliability of novel photovoltaic (PV) hardware and systems through laboratory and field testing. The focus of...

  20. Sequential addition of short DNA oligos in DNA-polymerase-based...

    Office of Scientific and Technical Information (OSTI)

    and combining the multiplicity of DNA sequence segments with at least one polymerase enzyme wherein the multiplicity of DNA sequence segments join to produce the DNA molecule of...

  1. Human Genome Research: Decoding DNA

    Office of Scientific and Technical Information (OSTI)

    ... Speeding Up the Process of Gene Discovery Engineered Enzyme Accelerates DNA Sequencing Putting a Virus to Practical Use DOE Joint Genome Institute The Human Genome Project: ...

  2. DNA attachment to support structures

    DOE Patents [OSTI]

    Balhorn, Rodney L.; Barry, Christopher H.

    2002-01-01

    Microscopic beads or other structures are attached to nucleic acids (DNA) using a terminal transferase. The transferase adds labeled dideoxy nucleotide bases to the ends of linear strands of DNA. The labels, such as the antigens digoxigenin and biotin, bind to the antibody compounds or other appropriate complementary ligands, which are bound to the microscopic beads or other support structures. The method does not require the synthesis of a synthetic oligonucleotide probe. The method can be used to tag or label DNA even when the DNA has an unknown sequence, has blunt ends, or is a very large fragment (e.g., >500 kilobase pairs).

  3. Human Genome Research: Decoding DNA

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Human Genome Research: Decoding DNA Resources with Additional Information Charles DeLisi ... role in proposing and initiating the Human Genome Program in 1986. The U.S. ...

  4. Method for assaying clustered DNA damages

    DOE Patents [OSTI]

    Sutherland, Betsy M.

    2004-09-07

    Disclosed is a method for detecting and quantifying clustered damages in DNA. In this method, a first aliquot of the DNA to be tested for clustered damages with one or more lesion-specific cleaving reagents under conditions appropriate for cleavage of the DNA to produce single-strand nicks in the DNA at sites of damage lesions. The number average molecular length (Ln) of double stranded DNA is then quantitatively determined for the treated DNA. The number average molecular length (Ln) of double stranded DNA is also quantitatively determined for a second, untreated aliquot of the DNA. The frequency of clustered damages (.PHI..sub.c) in the DNA is then calculated.

  5. Technology Roadmap Analysis 2013: Assessing Automotive Technology...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Roadmap Analysis 2013: Assessing Automotive Technology R&D Relevant to DOE Power Electronics Cost Targets Technology Roadmap Analysis 2013: Assessing Automotive Technology R&D ...

  6. When DNA Needs to Stand Up and Be Counted

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    DNA microarrays are small metal, glass, or silicon chips covered with patterns of short single-stranded DNA (ssDNA). These "DNA chips" are revolutionizing biotechnology, allowing ...

  7. Building Technologies Office Overview

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Roland Risser Director, Building Technologies Office Building Technologies Office Energy Efficiency Starts Here. 2 Building Technologies Office Integrated Approach: Improving ...

  8. Information Technology - DOE Directives, Delegations, and Requirements

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Information Technology

  9. Plasma technology

    SciTech Connect (OSTI)

    Herlitz, H.G.

    1986-11-01

    This paper describes the uses of plasma technology for the thermal destruction of hazardous wastes such as PCBs, dioxins, hydrocarbons, military chemicals and biological materials; for metals recovery from steel making dusts. One advantage of the process is that destruction of wastes can be carried out on site. Systems in several countries use the excess thermal energy for district heating.

  10. (Environmental technology)

    SciTech Connect (OSTI)

    Boston, H.L.

    1990-10-12

    The traveler participated in a conference on environmental technology in Paris, sponsored by the US Embassy-Paris, US Environmental Protection Agency (EPA), the French Environmental Ministry, and others. The traveler sat on a panel for environmental aspects of energy technology and made a presentation on the potential contributions of Oak Ridge National Laboratory (ORNL) to a planned French-American Environmental Technologies Institute in Chattanooga, Tennessee, and Evry, France. This institute would provide opportunities for international cooperation on environmental issues and technology transfer related to environmental protection, monitoring, and restoration at US Department of Energy (DOE) facilities. The traveler also attended the Fourth International Conference on Environmental Contamination in Barcelona. Conference topics included environmental chemistry, land disposal of wastes, treatment of toxic wastes, micropollutants, trace organics, artificial radionuclides in the environment, and the use biomonitoring and biosystems for environmental assessment. The traveler presented a paper on The Fate of Radionuclides in Sewage Sludge Applied to Land.'' Those findings corresponded well with results from studies addressing the fate of fallout radionuclides from the Chernobyl nuclear accident. There was an exchange of new information on a number of topics of interest to DOE waste management and environmental restoration needs.

  11. Manufacturing technologies

    SciTech Connect (OSTI)

    1995-09-01

    The Manufacturing Technologies Center is an integral part of Sandia National Laboratories, a multiprogram engineering and science laboratory, operated for the Department of Energy (DOE) with major facilities at Albuquerque, New Mexico, and Livermore, California. Our Center is at the core of Sandia`s Advanced Manufacturing effort which spans the entire product realization process.

  12. The 125 MW Upper Mahiao geothermal power plant

    SciTech Connect (OSTI)

    Forte, N.

    1996-12-31

    The 125 MW Upper Mahiao power plant, the first geothermal power project to be financed under a Build-Own-Operate-and-Transfer (BOOT) arrangement in the Philippines, expected to complete its start-up testing in August of this year. This plant uses Ormat`s environmentally benign technology and is both the largest geothermal steam/binary combined cycle plant as well as the largest geothermal power plant utilizing air cooled condensers. The Ormat designed and constructed plant was developed under a fast track program, with some two years from the April 1994 contract signing through design, engineering, construction and startup. The plant is owned and operated by a subsidiary of CalEnergy Co., Inc. and supplies power to PNOC-Energy Development Corporation for the National Power Corporation (Napocor) national power grid in the Philippines.

  13. DNA Sequence Determinants Controlling Affinity, Stability and...

    Office of Scientific and Technical Information (OSTI)

    the Nucleoid Protein Fis Citation Details In-Document Search Title: DNA Sequence Determinants Controlling Affinity, Stability and Shape of DNA Complexes Bound by the Nucleoid ...

  14. The Initiation of Bacterial DNA Replication

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The Initiation of Bacterial DNA Replication Print For the first time, scientists have determined the structure of the initiator of bacterial DNA replication. It is already known...

  15. The Initiation of Bacterial DNA Replication

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The Initiation of Bacterial DNA Replication The Initiation of Bacterial DNA Replication Print Wednesday, 31 January 2007 00:00 For the first time, scientists have determined the...

  16. Normalized cDNA libraries

    DOE Patents [OSTI]

    Soares, M.B.; Efstratiadis, A.

    1997-06-10

    This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3{prime} noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library. 4 figs.

  17. Normalized cDNA libraries

    DOE Patents [OSTI]

    Soares, Marcelo B.; Efstratiadis, Argiris

    1997-01-01

    This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3' noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library.

  18. Sequence independent amplification of DNA

    DOE Patents [OSTI]

    Bohlander, S.K.

    1998-03-24

    The present invention is a rapid sequence-independent amplification procedure (SIA). Even minute amounts of DNA from various sources can be amplified independent of any sequence requirements of the DNA or any a priori knowledge of any sequence characteristics of the DNA to be amplified. This method allows, for example, the sequence independent amplification of microdissected chromosomal material and the reliable construction of high quality fluorescent in situ hybridization (FISH) probes from YACs or from other sources. These probes can be used to localize YACs on metaphase chromosomes but also--with high efficiency--in interphase nuclei. 25 figs.

  19. Sequence independent amplification of DNA

    DOE Patents [OSTI]

    Bohlander, Stefan K.

    1998-01-01

    The present invention is a rapid sequence-independent amplification procedure (SIA). Even minute amounts of DNA from various sources can be amplified independent of any sequence requirements of the DNA or any a priori knowledge of any sequence characteristics of the DNA to be amplified. This method allows, for example the sequence independent amplification of microdissected chromosomal material and the reliable construction of high quality fluorescent in situ hybridization (FISH) probes from YACs or from other sources. These probes can be used to localize YACs on metaphase chromosomes but also--with high efficiency--in interphase nuclei.

  20. CSP technology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    technology - Sandia Energy Energy Search Icon Sandia Home Locations Contact Us Employee Locator Energy & Climate Secure & Sustainable Energy Future Stationary Power Energy Conversion Efficiency Solar Energy Wind Energy Water Power Supercritical CO2 Geothermal Natural Gas Safety, Security & Resilience of the Energy Infrastructure Energy Storage Nuclear Power & Engineering Grid Modernization Battery Testing Nuclear Energy Defense Waste Management Programs Advanced Nuclear Energy

  1. Technology disrupted

    SciTech Connect (OSTI)

    Papatheodorou, Y.

    2007-02-15

    Three years ago, the author presented a report on power generation technologies which in summary said 'no technology available today has the potential of becoming transformational or disruptive in the next five to ten years'. In 2006 the company completed another strategic view research report covering the electric power, oil, gas and unconventional energy industries and manufacturing industry. This article summarises the strategic view findings and then revisits some of the scenarios presented in 2003. The cost per megawatt-hour of the alternatives is given for plants ordered in 2005 and then in 2025. The issue of greenhouse gas regulation is dealt with through carbon sequestration and carbon allowances or an equivalent carbon tax. Results reveal substantial variability through nuclear power, hydro, wind, geothermal and biomass remain competitive through every scenario. Greenhouse gas scenario analysis shows coal still be viable, albeit less competitive against nuclear and renewable technologies. A carbon tax or allowance at $24 per metric ton has the same effect on IGCC cost as a sequestration mandate. However, the latter would hurt gas plants much more than a tax or allowance. Sequestering CO{sub 2} from a gas plant is almost as costly per megawatt-hour as for coal. 5 refs., 5 figs., 5 tabs.

  2. Building Technologies Office Overview

    SciTech Connect (OSTI)

    2013-04-01

    Building Technologies Office Overview Presentation for the 2013 Building Technologies Office's Program Peer Review

  3. Using DNA to Build Nanomaterials

    DOE R&D Accomplishments [OSTI]

    Walsh, Karen McNulty

    2011-05-09

    Scientists use complementary strands of synthetic DNA to build functional materials from the bottom up. Future applications include biosensors, optical nano-devices, and new kinds of solar cells.

  4. TECHNOLOGY TRANSFER

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    404-NOV. 1, 2000 TECHNOLOGY TRANSFER COMMERCIALIZATION ACT OF 2000 VerDate 11-MAY-2000 04:52 Nov 16, 2000 Jkt 089139 PO 00000 Frm 00001 Fmt 6579 Sfmt 6579 E:\PUBLAW\PUBL404.106 APPS27 PsN: PUBL404 114 STAT. 1742 PUBLIC LAW 106-404-NOV. 1, 2000 Public Law 106-404 106th Congress An Act To improve the ability of Federal agencies to license federally owned inventions. Be it enacted by the Senate and House of Representatives of the United States of America in Congress assembled, SECTION 1. SHORT

  5. Science & Technology Review September 2009

    SciTech Connect (OSTI)

    Bearinger, J P

    2009-07-24

    This month's issue has the following articles: (1) Remembering the Laboratory's First Director - Commentary by Harold Brown; (2) Herbert F. York (1921-2009): A Life of Firsts, an Ambassador for Peace - The Laboratory's first director, who died on May 19, 2009, used his expertise in science and technology to advance arms control and prevent nuclear war; (3) Searching for Life in Extreme Environments - DNA will help researchers discover new marine species and prepare to search for life on other planets; (4) Energy Goes with the Flow - Lawrence Livermore is one of the few organizations that distills the big picture about energy resources and use into a concise diagram; and (5) The Radiant Side of Sound - An experimental method that converts sound waves into light may lead to new technologies for scientific and industrial applications.

  6. Cellular responses to environmental DNA damage

    SciTech Connect (OSTI)

    Not Available

    1994-08-01

    This volume contains the proceedings of the conference entitled Cellular Responses to Environmental DNA Damage held in Banff,Alberta December 1--6, 1991. The conference addresses various aspects of DNA repair in sessions titled DNA repair; Basic Mechanisms; Lesions; Systems; Inducible Responses; Mutagenesis; Human Population Response Heterogeneity; Intragenomic DNA Repair Heterogeneity; DNA Repair Gene Cloning; Aging; Human Genetic Disease; and Carcinogenesis. Individual papers are represented as abstracts of about one page in length.

  7. DNA polymorphism identity determination using flow cytometry

    DOE Patents [OSTI]

    Nolan, John P.; White, P. Scott; Cai, Hong

    2001-01-01

    DNA polymorphism identity determination using flow cytometry. Primers designed to be immobilized on microspheres are allowed to anneal to the DNA strand under investigation, and are extended by either DNA polymerase using fluorescent dideoxynucleotides or ligated by DNA ligase to fluorescent reporter oligonucleotides. The fluorescence of either the dideoxynucleotide or the reporter oligonucleotide attached to the immobilized primer is measured by flow cytometry, thereby identifying the nucleotide polymorphism on the DNA strand.

  8. Interconnecting gold islands with DNA origami

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Interconnecting gold islands with DNA origami Authors: Ding, B., Wu, H., Xu, W., Zhao, Z., Liu, Y., Yu, H., and Yan, H. Title: Interconnecting gold islands with DNA origami Source: Nano Lett. Year: 2010 Volume: 10 Pages: 5065-5069 ABSTRACT: Scaffolded DNA origami has recently emerged as a versatile, programmable method to fold DNA into arbitrarily shaped nanostructures that are spatially addressable, with sub-10-nm resolution. Toward functional DNA nanotechnology, one of the key challenges is to

  9. DNA analysis conference in Santa Fe

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    DNA analysis conference in Santa Fe DNA analysis conference in Santa Fe Los Alamos National Laboratory is hosting a DNA sequence analysis and bioinformatics event, the 10th annual Sequencing, Finishing and Analysis in the Future (SFAF) workshop. May 27, 2015 DNA extracted from a soil sample is stored in a small vial of clear liquid. In general, living cells function by using the sequences of bases in their DNA as a blueprint for assembling proteins. A particularly important type of protein is

  10. Building Technologies Office Window and Envelope Technologies...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Building Technologies Office Window and Envelope Technologies Emerging Technologies R&D Program Karma Sawyer, Ph.D. karma.sawyer@ee.doe.gov BTO Goal Reduce building energy use by ...

  11. NETL Technologies Recognized for Technology Development, Transfer |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Recognized for Technology Development, Transfer NETL Technologies Recognized for Technology Development, Transfer October 25, 2013 - 1:31pm Addthis Did you know? The Federal Laboratory Consortium for Technology Transfer is the nationwide network of federal laboratories that provides the forum to develop strategies and opportunities for linking laboratory mission technologies and expertise with the marketplace. In consonance with the Federal Technology Transfer Act of

  12. High Impact Technology Catalyst: Technology Deployment Strategies |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Catalyst: Technology Deployment Strategies High Impact Technology Catalyst: Technology Deployment Strategies The Energy Department released the High Impact Technology Catalyst: Technology Deployment Strategies to serve as an overview of the HIT Catalyst program activities, including a summary of the selection process undertaken to identify, evaluate and prioritize the current HITs, descriptions of the technologies and markets for each HIT, and plans for deployment. High

  13. Vehicle Technologies Office: 2014 Electric Drive Technologies...

    Energy Savers [EERE]

    Electric Drive Technologies Annual Progress Report Vehicle ... FY14EDTAnnualReport.pdf (15.14 MB) More Documents & Publications Vehicle Technologies Office: 2015 ...

  14. Vehicle Technologies Office: 2009 Advanced Vehicle Technology...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Well-to-Wheels Analysis of Energy Use and Greenhouse Gas Emissions of Plug-In Hybrid Electric Vehicles Vehicle Technologies Office: 2008 Advanced Vehicle Technology Analysis and ...

  15. Vehicle Technologies Office: 2015 Electric Drive Technologies...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    automotive technologies under development. Research is focused on developing power electronics (PE), electric motor, and traction drive system (TDS) technologies that will ...

  16. Hydrogen Technologies Group

    SciTech Connect (OSTI)

    Not Available

    2008-03-01

    The Hydrogen Technologies Group at the National Renewable Energy Laboratory advances the Hydrogen Technologies and Systems Center's mission by researching a variety of hydrogen technologies.

  17. Nuclear Science & Technology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Nuclear Science & Technology Nuclear Science & Technology1354608000000Nuclear Science & TechnologySome of these resources are LANL-only and will require Remote Access. No...

  18. NREL: Technology Transfer - Ombuds

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Technology Transfer Ombuds NREL's Technology Transfer Ombuds offers an informal process to help resolve issues and concerns regarding the laboratory's technology partnership,...

  19. Stacking interactions and DNA intercalation

    SciTech Connect (OSTI)

    Li, Dr. Shen; Cooper, Valentino R; Thonhauser, Prof. Timo; Lundqvist, Prof. Bengt I.; Langreth, David C.

    2009-01-01

    The relationship between stacking interactions and the intercalation of proflavine and ellipticine within DNA is investigated using a nonempirical van der Waals density functional for the correlation energy. Our results, employing a binary stack model, highlight fundamental, qualitative differences between base-pair base-pair interactions and that of the stacked intercalator base pair system. Most notable result is the paucity of torque which so distinctively defines the Twist of DNA. Surprisingly, this model, when combined with a constraint on the twist of the surrounding base-pair steps to match the observed unwinding of the sugar-phosphate backbone, was sufficient for explaining the experimentally observed proflavine intercalator configuration. Our extensive mapping of the potential energy surface of base-pair intercalator interactions can provide valuable information for future nonempirical studies of DNA intercalation dynamics.

  20. Apparatus for improved DNA sequencing

    DOE Patents [OSTI]

    Douthart, Richard J.; Crowell, Shannon L.

    1996-01-01

    This invention is a means for the rapid sequencing of DNA samples. More specifically, it consists of a new design direct blotting electrophoresis unit. The DNA sequence is deposited on a membrane attached to a rotating drum. Initial data compaction is facilitated by the use of a machined multi-channeled plate called a ribbon channel plate. Each channel is an isolated mini gel system much like a gel filled capillary. The system as a whole, however, is in a slab gel like format with the advantages of uniformity and easy reusability. The system can be used in different embodiments. The drum system is unique in that after deposition the drum rotates the deposited DNA into a large non-buffer open space where processing and detection can occur. The drum can also be removed in toto to special workstations for downstream processing, multiplexing and detection.

  1. Apparatus for improved DNA sequencing

    DOE Patents [OSTI]

    Douthart, R.J.; Crowell, S.L.

    1996-05-07

    This invention is a means for the rapid sequencing of DNA samples. More specifically, it consists of a new design direct blotting electrophoresis unit. The DNA sequence is deposited on a membrane attached to a rotating drum. Initial data compaction is facilitated by the use of a machined multi-channeled plate called a ribbon channel plate. Each channel is an isolated mini gel system much like a gel filled capillary. The system as a whole, however, is in a slab gel like format with the advantages of uniformity and easy reusability. The system can be used in different embodiments. The drum system is unique in that after deposition the drum rotates the deposited DNA into a large non-buffer open space where processing and detection can occur. The drum can also be removed in toto to special workstations for downstream processing, multiplexing and detection. 18 figs.

  2. Delineating Rearrangements in Single Yeast Artificial Chromosomes by Quantitative DNA Fiber Mapping

    SciTech Connect (OSTI)

    Weier, Heinz-Ulrich G.; Greulich-Bode, Karin M.; Wu, Jenny; Duell, Thomas

    2009-09-18

    Cloning of large chunks of human genomic DNA in recombinant systems such as yeast or bacterial artificial chromosomes has greatly facilitated the construction of physical maps, the positional cloning of disease genes or the preparation of patient-specific DNA probes for diagnostic purposes. For this process to work efficiently, the DNA cloning process and subsequent clone propagation need to maintain stable inserts that are neither deleted nor otherwise rearranged. Some regions of the human genome; however, appear to have a higher propensity than others to rearrange in any host system. Thus, techniques to detect and accurately characterize such rearrangements need to be developed. We developed a technique termed 'Quantitative DNA Fiber Mapping (QDFM)' that allows accurate tagging of sequence elements of interest with near kilobase accuracy and optimized it for delineation of rearrangements in recombinant DNA clones. This paper demonstrates the power of this microscopic approach by investigating YAC rearrangements. In our examples, high-resolution physical maps for regions within the immunoglobulin lambda variant gene cluster were constructed for three different YAC clones carrying deletions of 95 kb and more. Rearrangements within YACs could be demonstrated unambiguously by pairwise mapping of cosmids along YAC DNA molecules. When coverage by YAC clones was not available, distances between cosmid clones were estimated by hybridization of cosmids onto DNA fibers prepared from human genomic DNA. In addition, the QDFM technology provides essential information about clone stability facilitating closure of the maps of the human genome as well as those of model organisms.

  3. Technology Partnership Agreements | NREL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Technology Investment Agreements Technology Investment Agreements Guidance Policy Flash 2006-31 - Technology Investment Agreements Financial Assistance Letter 2006-03 - Guidance for Awarding Technology Investment Agreements Final Rule - Financial Assistance Regulations - Technology Investment Agreements Templates Company Template (Expenditure-Based) Consortium Template (Expenditure-Based) Company Template (Fixed Support) Consortium Support (Fixed Support) Training Technology Investment

  4. Chromosome specific repetitive DNA sequences

    DOE Patents [OSTI]

    Moyzis, Robert K.; Meyne, Julianne

    1991-01-01

    A method is provided for determining specific nucleotide sequences useful in forming a probe which can identify specific chromosomes, preferably through in situ hybridization within the cell itself. In one embodiment, chromosome preferential nucleotide sequences are first determined from a library of recombinant DNA clones having families of repetitive sequences. Library clones are identified with a low homology with a sequence of repetitive DNA families to which the first clones respectively belong and variant sequences are then identified by selecting clones having a pattern of hybridization with genomic DNA dissimilar to the hybridization pattern shown by the respective families. In another embodiment, variant sequences are selected from a sequence of a known repetitive DNA family. The selected variant sequence is classified as chromosome specific, chromosome preferential, or chromosome nonspecific. Sequences which are classified as chromosome preferential are further sequenced and regions are identified having a low homology with other regions of the chromosome preferential sequence or with known sequences of other family me This invention is the result of a contract with the Department of Energy (Contract No. W-7405-ENG-36).

  5. Structural Origins of DNA Target Selection and Nucleobase Extrusion...

    Office of Scientific and Technical Information (OSTI)

    of DNA Target Selection and Nucleobase Extrusion by a DNA Cytosine Methyltransferase Citation Details In-Document Search Title: Structural Origins of DNA Target Selection ...

  6. Method for priming and DNA sequencing (Patent Application) |...

    Office of Scientific and Technical Information (OSTI)

    Subject: 55 BIOLOGY AND MEDICINE, BASIC STUDIES; DNA SEQUENCING; DNA SEQUENCERS; EXPERIMENTAL DATA; OLIGONUCLEOTIDES; DNA; MOLECULAR BIOLOGY; MOLECULAR STRUCTURE Word Cloud More ...

  7. A DNA tweezer-actuated enzyme nanoreactor

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    A DNA tweezer-actuated enzyme nanoreactor Authors: Liu, M., Fu, J., Hejesen, C., Yang, Y., Woodbury, N.W., Gothelf, K., Liu, Y., and Yan, H. Title: A DNA tweezer-actuated enzyme...

  8. Amplification of chromosomal DNA in situ

    DOE Patents [OSTI]

    Christian, Allen T.; Coleman, Matthew A.; Tucker, James D.

    2002-01-01

    Amplification of chromosomal DNA in situ to increase the amount of DNA associated with a chromosome or chromosome region is described. The amplification of chromosomal DNA in situ provides for the synthesis of Fluorescence in situ Hybridization (FISH) painting probes from single dissected chromosome fragments, the production of cDNA libraries from low copy mRNAs and improved in Comparative Genomic Hybridization (CGH) procedures.

  9. Antibody specific for a DNA repair protein

    DOE Patents [OSTI]

    Petrini, John H.; Morgan, William Francis; Maser, Richard Scott; Carney, James Patrick

    2006-07-11

    An isolated and purified DNA molecule encoding a DNA repair protein, p95, is provided, as is isolated and purified p95. Also provided are methods of detecting p95 and DNA encoding p95. The invention further provides p95 knock-out mice.

  10. Probe and method for DNA detection

    DOE Patents [OSTI]

    Yeh, Hsin-Chih; Werner, James Henry; Sharma, Jaswinder Kumar; Martinez, Jennifer Suzanne

    2013-07-02

    A hybridization probe containing two linear strands of DNA lights up upon hybridization to a target DNA using silver nanoclusters that have been templated onto one of the DNA strands. Hybridization induces proximity between the nanoclusters on one strand and an overhang on the other strand, which results in enhanced fluorescence emission from the nanoclusters.

  11. Phylogenetic Analysis of Shewanella Strains by DNA Relatedness...

    Office of Scientific and Technical Information (OSTI)

    These results indicate that WCGA-based DNA-DNA hybridization is an idea alternative of conventional DNA-DNA hybridization methods and it is superior to the phylogenetics methods ...

  12. NV-020-08-DNA-52 | Open Energy Information

    Open Energy Info (EERE)

    DNA-52 Jump to: navigation, search NEPA Document Collection for: NV-020-08-DNA-52 DNA for GeothermalExploration, DNA for Thermal Gradient Hole at Gavvs Valley for Geothermal...

  13. Innovative Technologies for Bioenergy Technologies Incubator...

    Broader source: Energy.gov (indexed) [DOE]

    00PM EDT Online The Innovative Technologies for Bioenergy Technologies Incubator 2 FOA Informational Webinar will be held Wednesday, September 2, 1:00 p.m.-2:00 p.m. ET. Standard...

  14. Particle sizer and DNA sequencer

    DOE Patents [OSTI]

    Olivares, Jose A.; Stark, Peter C.

    2005-09-13

    An electrophoretic device separates and detects particles such as DNA fragments, proteins, and the like. The device has a capillary which is coated with a coating with a low refractive index such as Teflon.RTM. AF. A sample of particles is fluorescently labeled and injected into the capillary. The capillary is filled with an electrolyte buffer solution. An electrical field is applied across the capillary causing the particles to migrate from a first end of the capillary to a second end of the capillary. A detector light beam is then scanned along the length of the capillary to detect the location of the separated particles. The device is amenable to a high throughput system by providing additional capillaries. The device can also be used to determine the actual size of the particles and for DNA sequencing.

  15. Plasma technology directory

    SciTech Connect (OSTI)

    Ward, P.P.; Dybwad, G.L.

    1995-03-01

    The Plasma Technology Directory has two main goals: (1) promote, coordinate, and share plasma technology experience and equipment within the Department of Energy; and (2) facilitate technology transfer to the commercial sector where appropriate. Personnel are averaged first by Laboratory and next by technology area. The technology areas are accelerators, cleaning and etching deposition, diagnostics, and modeling.

  16. Vehicle Technologies Office Propulsion Materials Technologies

    Broader source: Energy.gov (indexed) [DOE]

    Construction | Department of Energy UQM Technologies, Inc. at the 2016 DOE Vehicle Technologies Office and Hydrogen and Fuel Cells Program Annual Merit Review and Peer Evaluation Meeting about Electric Drive Systems edt044_ley_2016_o_web.pdf (1.13 MB) More Documents & Publications Vehicle Technologies Office Merit Review 2015: Unique Lanthide-Free Motor Construction Vehicle Technologies Office Merit Review 2014: Unique Lanthide-Free Motor Construction Unique Lanthide-Free Motor

  17. Binding of undamaged double stranded DNA to vaccinia virus uracil-DNA glycosylase

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Schormann, Norbert; Banerjee, Surajit; Ricciardi, Robert; Chattopadhyay, Debasish

    2015-06-02

    Background: Uracil-DNA glycosylases are evolutionarily conserved DNA repair enzymes. However, vaccinia virus uracil-DNA glycosylase (known as D4), also serves as an intrinsic and essential component of the processive DNA polymerase complex during DNA replication. In this complex D4 binds to a unique poxvirus specific protein A20 which tethers it to the DNA polymerase. At the replication fork the DNA scanning and repair function of D4 is coupled with DNA replication. So far, DNA-binding to D4 has not been structurally characterized. Results: This manuscript describes the first structure of a DNA-complex of a uracil-DNA glycosylase from the poxvirus family. This alsomore » represents the first structure of a uracil DNA glycosylase in complex with an undamaged DNA. In the asymmetric unit two D4 subunits bind simultaneously to complementary strands of the DNA double helix. Each D4 subunit interacts mainly with the central region of one strand. DNA binds to the opposite side of the A20-binding surface on D4. In comparison of the present structure with the structure of uracil-containing DNA-bound human uracil-DNA glycosylase suggests that for DNA binding and uracil removal D4 employs a unique set of residues and motifs that are highly conserved within the poxvirus family but different in other organisms. Conclusion: The first structure of D4 bound to a truly non-specific undamaged double-stranded DNA suggests that initial binding of DNA may involve multiple non-specific interactions between the protein and the phosphate backbone.« less

  18. Binding of undamaged double stranded DNA to vaccinia virus uracil-DNA glycosylase

    SciTech Connect (OSTI)

    Schormann, Norbert; Banerjee, Surajit; Ricciardi, Robert; Chattopadhyay, Debasish

    2015-06-02

    Background: Uracil-DNA glycosylases are evolutionarily conserved DNA repair enzymes. However, vaccinia virus uracil-DNA glycosylase (known as D4), also serves as an intrinsic and essential component of the processive DNA polymerase complex during DNA replication. In this complex D4 binds to a unique poxvirus specific protein A20 which tethers it to the DNA polymerase. At the replication fork the DNA scanning and repair function of D4 is coupled with DNA replication. So far, DNA-binding to D4 has not been structurally characterized. Results: This manuscript describes the first structure of a DNA-complex of a uracil-DNA glycosylase from the poxvirus family. This also represents the first structure of a uracil DNA glycosylase in complex with an undamaged DNA. In the asymmetric unit two D4 subunits bind simultaneously to complementary strands of the DNA double helix. Each D4 subunit interacts mainly with the central region of one strand. DNA binds to the opposite side of the A20-binding surface on D4. In comparison of the present structure with the structure of uracil-containing DNA-bound human uracil-DNA glycosylase suggests that for DNA binding and uracil removal D4 employs a unique set of residues and motifs that are highly conserved within the poxvirus family but different in other organisms. Conclusion: The first structure of D4 bound to a truly non-specific undamaged double-stranded DNA suggests that initial binding of DNA may involve multiple non-specific interactions between the protein and the phosphate backbone.

  19. Channel plate for DNA sequencing

    DOE Patents [OSTI]

    Douthart, Richard J.; Crowell, Shannon L.

    1998-01-01

    This invention is a channel plate that facilitates data compaction in DNA sequencing. The channel plate has a length, a width and a thickness, and further has a plurality of channels that are parallel. Each channel has a depth partially through the thickness of the channel plate. Additionally an interface edge permits electrical communication across an interface through a buffer to a deposition membrane surface.

  20. Channel plate for DNA sequencing

    DOE Patents [OSTI]

    Douthart, R.J.; Crowell, S.L.

    1998-01-13

    This invention is a channel plate that facilitates data compaction in DNA sequencing. The channel plate has a length, a width and a thickness, and further has a plurality of channels that are parallel. Each channel has a depth partially through the thickness of the channel plate. Additionally an interface edge permits electrical communication across an interface through a buffer to a deposition membrane surface. 15 figs.

  1. The Initiation of Bacterial DNA Replication

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The Initiation of Bacterial DNA Replication The Initiation of Bacterial DNA Replication Print Wednesday, 31 January 2007 00:00 For the first time, scientists have determined the structure of the initiator of bacterial DNA replication. It is already known that such replication is controlled by a protein known as DnaA, a member of the AAA+ superfamily of ATPases. What has now been discovered is that the core of the initiator is not the closed-ring structure expected for this system. Instead, DnaA

  2. Method for sequencing DNA base pairs

    DOE Patents [OSTI]

    Sessler, Andrew M.; Dawson, John

    1993-01-01

    The base pairs of a DNA structure are sequenced with the use of a scanning tunneling microscope (STM). The DNA structure is scanned by the STM probe tip, and, as it is being scanned, the DNA structure is separately subjected to a sequence of infrared radiation from four different sources, each source being selected to preferentially excite one of the four different bases in the DNA structure. Each particular base being scanned is subjected to such sequence of infrared radiation from the four different sources as that particular base is being scanned. The DNA structure as a whole is separately imaged for each subjection thereof to radiation from one only of each source.

  3. Microfluidic DNA sample preparation method and device

    DOE Patents [OSTI]

    Krulevitch, Peter A.; Miles, Robin R.; Wang, Xiao-Bo; Mariella, Raymond P.; Gascoyne, Peter R. C.; Balch, Joseph W.

    2002-01-01

    Manipulation of DNA molecules in solution has become an essential aspect of genetic analyses used for biomedical assays, the identification of hazardous bacterial agents, and in decoding the human genome. Currently, most of the steps involved in preparing a DNA sample for analysis are performed manually and are time, labor, and equipment intensive. These steps include extraction of the DNA from spores or cells, separation of the DNA from other particles and molecules in the solution (e.g. dust, smoke, cell/spore debris, and proteins), and separation of the DNA itself into strands of specific lengths. Dielectrophoresis (DEP), a phenomenon whereby polarizable particles move in response to a gradient in electric field, can be used to manipulate and separate DNA in an automated fashion, considerably reducing the time and expense involved in DNA analyses, as well as allowing for the miniaturization of DNA analysis instruments. These applications include direct transport of DNA, trapping of DNA to allow for its separation from other particles or molecules in the solution, and the separation of DNA into strands of varying lengths.

  4. Contributing Data to the Fleet DNA Project (Brochure)

    SciTech Connect (OSTI)

    Not Available

    2014-09-01

    The Fleet DNA clearinghouse of commercial fleet transportation data helps vehicle manufacturers and developers optimize vehicle designs and helps fleet managers choose advanced technologies for their fleets. This online tool - available at www.nrel.gov/fleetdna - provides data summaries and visualizations similar to real-world 'genetics' for medium- and heavy-duty commercial fleet vehicles operating within a variety of vocations. To contribute your fleet data, please contact Adam Duran of the U.S. Department of Energy's National Renewable Energy Laboratory (NREL) at adam.duran@nrel.gov or 303-275-4586.

  5. NATIONAL ENERGY TECHNOLOGY LABORATORY Technology Transfer NETL Sorbent Technologies

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Sorbent Technologies Licensed for Use in Biomass-to- Biofuel Conversion Process with Carbon Capture and Storage Success Story The U.S. Department of Energy's National Energy Technology Laboratory (NETL) has granted a license for two of its patented sorbent technologies: carbon dioxide (CO 2 ) removal and water-gas shift (WGS) reaction enhancement to CogniTek Management Systems "CogniTek," a renewable energy systems developer. CogniTek plans to implement a regenerable magnesium sorbent,

  6. Forest products technologies

    SciTech Connect (OSTI)

    None, None

    2006-07-18

    Report highlights DOE Industrial Technology Program co-funded R&D resulting in commercial energy-efficient technologies and emerging technologies helping the forest products industry save energy.

  7. NETL: SOFC Core Technology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Core Technology Core Technology-This key technology conducts applied research and development on technologies - exclusive of the cell components - that improve the cost, performance, robustness, reliability, and endurance of SOFC stack or balance-of-plant (BOP) technology. Projects in the Core Technology portfolio focus on interconnects and seals, identify and mitigate stack-related degradation, develop computational tools and models, and conduct laboratory- and bench-scale testing to improve

  8. EA-1849: Finding of No Significant Impact | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    Ormat Nevada Northern Nevada Geothermal Power Plant Projects: Loan Guarantee for ORMAT LLC's Tuscarora Geothermal Power Plant, Elko County, Nevada; Jersey Valley Geothermal ...

  9. Desert Peak EGS Project

    Broader source: Energy.gov (indexed) [DOE]

    Ethan Chabora GeothermEx, a Schlumberger Company Ezra Zemach Ormat Nevada Inc. ... Collaborations * Project Leader: Ormat Nevada, Inc. - Co-Management: GeothermEx, Inc. - ...

  10. North Brawley Geothermal Power Plant | Open Energy Information

    Open Energy Info (EERE)

    Zone Plant Information Facility Type Binary Cycle Power Plant Owner Ormat Developer Ormat Energy Purchaser Southern California Edison Number of Units 5 Commercial Online Date 2010...

  11. DOI-BLM-NV-WO10-2014-0002-CX | Open Energy Information

    Open Energy Info (EERE)

    NVN-086871 Proposed Action Ormat Nevada, Inc. (Ormat) is proposing up to five exploratory temperature gradient (TG) well locations to assess the geothermal resource of the Baltazor...

  12. Morgantown Energy Technology Center, technology summary

    SciTech Connect (OSTI)

    Not Available

    1994-06-01

    This document has been prepared by the DOE Environmental Management (EM) Office of Technology Development (OTD) to highlight its research, development, demonstration, testing, and evaluation activities funded through the Morgantown Energy Technology Center (METC). Technologies and processes described have the potential to enhance DOE`s cleanup and waste management efforts, as well as improve US industry`s competitiveness in global environmental markets. METC`s R&D programs are focused on commercialization of technologies that will be carried out in the private sector. META has solicited two PRDAs for EM. The first, in the area of groundwater and soil technologies, resulted in twenty-one contact awards to private sector and university technology developers. The second PRDA solicited novel decontamination and decommissioning technologies and resulted in eighteen contract awards. In addition to the PRDAs, METC solicited the first EM ROA in 1993. The ROA solicited research in a broad range of EM-related topics including in situ remediation, characterization, sensors, and monitoring technologies, efficient separation technologies, mixed waste treatment technologies, and robotics. This document describes these technology development activities.

  13. Green Purchasing & Green Technology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Purchasing & Technology Goals 6 & 7: Green Purchasing & Green Technology Our goal is to purchase and use environmentally sustainable products whenever possible and to implement...

  14. Vehicle Technologies Office: News

    Broader source: Energy.gov [DOE]

    EERE intends to issue, on behalf of its Fuel Cell Technologies Office, a Funding Opportunity Announcement (FOA) entitled "Fuel Cell Technologies Incubator: Innovations in Fuel Cell and Hydrogen...

  15. CBI Technology Impact Framework

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    CBI Technology Impact Framework 2014 Building Technologies Office Peer Review Images courtesy CREE, True Manufacturing, A.O. Smith, Bernstein Associates, Cambridge Engineering, ...

  16. Hydropower Program Technology Overview

    SciTech Connect (OSTI)

    Not Available

    2001-10-01

    New fact sheets for the DOE Office of Power Technologies (OPT) that provide technology overviews, description of DOE programs, and market potential for each OPT program area.

  17. NREL: Technology Transfer - Contacts

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    you may have about NREL's technology transfer opportunities. Partnering with NREL Anne Miller, 303-384-7353 Licensing NREL Technologies Eric Payne, 303-275-3166 Printable Version...

  18. Technology Selection Process

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    technologies, including Technical Advisory Groups and the Energy Efficiency Technology Roadmap. Technical Advisory Groups E3T engages stakeholders of electric power industries in...

  19. First National Technology Center

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    First National Technology First National Technology Center Center Electronic Equipment - manufactured to withstand 8 milliseconds of voltage disruption CBEMA Curve - Chips ...

  20. Promising Technologies List

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    about promising new and underutilized energy-saving technologies available for Federal and commercial building sector deployment. To identify promising technologies,...

  1. TECHNOLOGY READINESS ASSESSMENT

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of affordable clean energy technology -Carbon Capture, Utilization, and Storage (CCUS) 2012 TECHNOLOGY ... of the nation's energy system and secure U.S. leadership ...

  2. DNA

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    LOS ALAMOS, N.M., Sept. 15, 2014-When this week's print issue of the journal Science comes out, a collective cheer will go up from New Mexico, Montana and even the Netherlands, ...

  3. Blue Spark Technologies formerly Thin Battery Technologies Inc...

    Open Energy Info (EERE)

    Spark Technologies formerly Thin Battery Technologies Inc Jump to: navigation, search Name: Blue Spark Technologies (formerly Thin Battery Technologies Inc.) Place: Westlake, Ohio...

  4. Technology Readiness Assessment (TRA)/Technology Maturation Plan...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Technology Readiness Assessment (TRA)Technology Maturation Plan (TMP) Process Guide Technology Readiness Assessment (TRA)Technology Maturation Plan (TMP) Process Guide This ...

  5. Sun Materials Technology aka Shanyang Technology | Open Energy...

    Open Energy Info (EERE)

    Technology aka Shanyang Technology Jump to: navigation, search Name: Sun Materials Technology (aka Shanyang Technology) Place: Yilan County, Taiwan Product: A US-Taiwan JV company...

  6. Quantum Fuel Systems Technologies Worldwide Inc Quantum Technologies...

    Open Energy Info (EERE)

    Fuel Systems Technologies Worldwide Inc Quantum Technologies Jump to: navigation, search Name: Quantum Fuel Systems Technologies Worldwide Inc (Quantum Technologies) Place: Irvine,...

  7. DOE Vehicle Technologies Program 2009 Merit Review Report - Technology...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Technology Integration and Education DOE Vehicle Technologies Program 2009 Merit Review Report - Technology Integration and Education Merit review of DOE Vehicle Technologies ...

  8. 2010 DOE EERE Vehicle Technologies Program Merit Review … Technology...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Technology Integration 2010 DOE EERE Vehicle Technologies Program Merit Review Technology Integration Technology integration merit review results PDF icon 2010amr08.pdf ...

  9. GT Solar Technologies formerly GT Equipment Technologies | Open...

    Open Energy Info (EERE)

    Technologies formerly GT Equipment Technologies Jump to: navigation, search Name: GT Solar Technologies (formerly GT Equipment Technologies) Place: Merrimack, New Hampshire...

  10. DNA Sequencing Using capillary Electrophoresis

    SciTech Connect (OSTI)

    Dr. Barry Karger

    2011-05-09

    The overall goal of this program was to develop capillary electrophoresis as the tool to be used to sequence for the first time the Human Genome. Our program was part of the Human Genome Project. In this work, we were highly successful and the replaceable polymer we developed, linear polyacrylamide, was used by the DOE sequencing lab in California to sequence a significant portion of the human genome using the MegaBase multiple capillary array electrophoresis instrument. In this final report, we summarize our efforts and success. We began our work by separating by capillary electrophoresis double strand oligonucleotides using cross-linked polyacrylamide gels in fused silica capillaries. This work showed the potential of the methodology. However, preparation of such cross-linked gel capillaries was difficult with poor reproducibility, and even more important, the columns were not very stable. We improved stability by using non-cross linked linear polyacrylamide. Here, the entangled linear chains could move when osmotic pressure (e.g. sample injection) was imposed on the polymer matrix. This relaxation of the polymer dissipated the stress in the column. Our next advance was to use significantly lower concentrations of the linear polyacrylamide that the polymer could be automatically blown out after each run and replaced with fresh linear polymer solution. In this way, a new column was available for each analytical run. Finally, while testing many linear polymers, we selected linear polyacrylamide as the best matrix as it was the most hydrophilic polymer available. Under our DOE program, we demonstrated initially the success of the linear polyacrylamide to separate double strand DNA. We note that the method is used even today to assay purity of double stranded DNA fragments. Our focus, of course, was on the separation of single stranded DNA for sequencing purposes. In one paper, we demonstrated the success of our approach in sequencing up to 500 bases. Other

  11. NREL: Technology Transfer - Commercialization Programs

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    303-275-3051. Printable Version Technology Transfer Home About Technology Transfer Technology Partnership Agreements Licensing Agreements Nondisclosure Agreements...

  12. DNA-guided nanoparticle assemblies

    DOE Patents [OSTI]

    Gang, Oleg; Nykypanchuk, Dmytro; Maye, Mathew; van der Lelie, Daniel

    2013-07-16

    In some embodiments, DNA-capped nanoparticles are used to define a degree of crystalline order in assemblies thereof. In some embodiments, thermodynamically reversible and stable body-centered cubic (bcc) structures, with particles occupying <.about.10% of the unit cell, are formed. Designs and pathways amenable to the crystallization of particle assemblies are identified. In some embodiments, a plasmonic crystal is provided. In some aspects, a method for controlling the properties of particle assemblages is provided. In some embodiments a catalyst is formed from nanoparticles linked by nucleic acid sequences and forming an open crystal structure with catalytically active agents attached to the crystal on its surface or in interstices.

  13. Photochemistry of psoralen-DNA adducts, biological effects of psoralen-DNA adducts, applications of psoralen-DNA photochemistry

    SciTech Connect (OSTI)

    Shi, Yun-bo

    1988-03-01

    This thesis consists of three main parts and totally eight chapters. In Part I, The author will present studies on the photochemistry of psoralen-DNA adducts, specifically, the wavelength dependencies for the photoreversals of thymidine-HMT (4'-hydroxymethyl-4, 5', 8-trimenthylpsoralen) monoadducts and diadduct and the same adducts incorporated in DNA helices and the wavelength dependecies for the photocrossslinking of thymidine-HMT monoadducts in double-stranded helices. In Part II, The author will report some biological effects of psoralen-DNA adducts, i.e., the effects on double-stranded DNA stability, DNA structure, and transcription by E. coli and T7 RNA polymerases. Finally, The author will focus on the applications of psoralen-DNA photochemistry to investigation of protein-DNA interaction during transcription, which includes the interaction of E. coli and T7 RNA polymerases with DNA in elongation complexes arrested at specific psoralen-DNA adduct sites as revealed by DNase I footprinting experiments. 123 refs., 52 figs., 12 tabs.

  14. Innovative Process Technologies

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Innovative Process Technologies Research Team Members Key Contacts Innovative Process Technologies Innovative Process Technologies is concerned with the development of innovative costeffective technologies that promote efficiency, environmental performance, availability of advanced energy systems, and the development of computational tools that shorten development timelines of advanced energy systems. NETL, working with members of the NETL-Regional University Alliance (NETL-RUA), will focus on

  15. Hydrogen delivery technology roadmap

    SciTech Connect (OSTI)

    None, None

    2005-11-15

    Document describing plan for research into and development of hydrogen delivery technology for transportation applications.

  16. Soil washing technology evaluation

    SciTech Connect (OSTI)

    Suer, A.

    1995-04-01

    Environmental Restoration Engineering (ERE) continues to review innovative, efficient, and cost effective technologies for SRS soil and/or groundwater remediation. As part of this effort, this technical evaluation provides review and the latest information on the technology for SRS soil remediation. Additional technology evaluation reports will be issued periodically to update these reports. The purpose of this report is to review the soil washing technology and its potential application to SRS soil remediation. To assess whether the Soil Washing technology is a viable option for SRS soil remediation, it is necessary to review the technology/process, technology advantages/limitations, performance, applications, and cost analysis.

  17. NATIONAL ENERGY TECHNOLOGY LABORATORY Technology Transfer

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The unique technology allows operators to optimize the processing to improve material yield, decrease energy use, and improve safety systems. Specialty metals, such as titanium or ...

  18. NATIONAL ENERGY TECHNOLOGY LABORATORY Technology Transfer Novel...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Novel PlatinumChromium Alloy for the Manufacture of Improved Coronary Stents Success Story NETL Technology Transfer Group techtransfer@netl.doe.gov Contact Partners A coronary...

  19. Materials Science and Technology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    MST Materials Science and Technology Providing world-leading, innovative, and agile materials science and technology solutions for national security missions. MST is metallurgy. The Materials Science and Technology Division provides scientific and technical leadership in materials science and technology for Los Alamos National Laboratory. READ MORE MST is engineered materials. The Materials Science and Technology Division provides scientific and technical leadership in materials science and

  20. TECHNOLOGY TRANSFER COORDINATORS

    Office of Energy Efficiency and Renewable Energy (EERE)

    Mark Hartney, Director of the Office of Strategic Planning, SLAC, discussed technology transfer at SLAC. Bob Hwang, Director, Transportation Energy Center, Combustion Research Facility, SNL presented on technology transfer at SNL. Elsie Quaite-Randall, Chief Technology Transfer Officer, Innovation and Partnerships Office, LBNL, presented on technology transfer at LBNL. Richard A. Rankin, Director, Industrial Partnerships Office and Economic Development Office (Interim), LLNL, presented on technology transfer at LLNL.

  1. NATIONAL ENERGY TECHNOLOGY LABORATORY Technology Transfer NETL Technology for Safer,

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Technology for Safer, Cleaner Corrosion-Protecting Metal Coatings Licensed by Pittsburgh Start-Up Success Story Corrosion-related issues cost the U.S. economy $276 billion a year. The Energy Department's National Energy Technology Laboratory (NETL) teamed up with Carnegie Mellon University (CMU) to create a revolutionary, cost-effective technology to reduce that impact-work that resulted in the creation of a new CMU/NETL spin-off that signed a licensing agreement with the laboratory in June. The

  2. DNA Origami: A History and Current Perspective

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Origami: A History and Current Perspective Authors: Nangreave, J., Han, D., Liu, Y., and Yan, H. Title: DNA Origami: A History and Current Perspective Source: Current Opinion in ...

  3. The Initiation of Bacterial DNA Replication

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The Initiation of Bacterial DNA Replication Print For the first time, scientists have determined the structure of the initiator of bacterial DNA replication. It is already known that such replication is controlled by a protein known as DnaA, a member of the AAA+ superfamily of ATPases. What has now been discovered is that the core of the initiator is not the closed-ring structure expected for this system. Instead, DnaA forms an open right-handed helix. In addition, the architecture indicates

  4. The Initiation of Bacterial DNA Replication

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The Initiation of Bacterial DNA Replication Print For the first time, scientists have determined the structure of the initiator of bacterial DNA replication. It is already known that such replication is controlled by a protein known as DnaA, a member of the AAA+ superfamily of ATPases. What has now been discovered is that the core of the initiator is not the closed-ring structure expected for this system. Instead, DnaA forms an open right-handed helix. In addition, the architecture indicates

  5. DNA sequencing using fluorescence background electroblotting membrane

    DOE Patents [OSTI]

    Caldwell, K.D.; Chu, T.J.; Pitt, W.G.

    1992-05-12

    A method for the multiplex sequencing on DNA is disclosed which comprises the electroblotting or specific base terminated DNA fragments, which have been resolved by gel electrophoresis, onto the surface of a neutral non-aromatic polymeric microporous membrane exhibiting low background fluorescence which has been surface modified to contain amino groups. Polypropylene membranes are preferably and the introduction of amino groups is accomplished by subjecting the membrane to radio or microwave frequency plasma discharge in the presence of an aminating agent, preferably ammonia. The membrane, containing physically adsorbed DNA fragments on its surface after the electroblotting, is then treated with crosslinking means such as UV radiation or a glutaraldehyde spray to chemically bind the DNA fragments to the membrane through amino groups contained on the surface. The DNA fragments chemically bound to the membrane are subjected to hybridization probing with a tagged probe specific to the sequence of the DNA fragments. The tagging may be by either fluorophores or radioisotopes. The tagged probes hybridized to the target DNA fragments are detected and read by laser induced fluorescence detection or autoradiograms. The use of aminated low fluorescent background membranes allows the use of fluorescent detection and reading even when the available amount of DNA to be sequenced is small. The DNA bound to the membranes may be reprobed numerous times. No Drawings

  6. DNA sequencing using fluorescence background electroblotting membrane

    DOE Patents [OSTI]

    Caldwell, Karin D.; Chu, Tun-Jen; Pitt, William G.

    1992-01-01

    A method for the multiplex sequencing on DNA is disclosed which comprises the electroblotting or specific base terminated DNA fragments, which have been resolved by gel electrophoresis, onto the surface of a neutral non-aromatic polymeric microporous membrane exhibiting low background fluorescence which has been surface modified to contain amino groups. Polypropylene membranes are preferably and the introduction of amino groups is accomplished by subjecting the membrane to radio or microwave frequency plasma discharge in the presence of an aminating agent, preferably ammonia. The membrane, containing physically adsorbed DNA fragments on its surface after the electroblotting, is then treated with crosslinking means such as UV radiation or a glutaraldehyde spray to chemically bind the DNA fragments to the membrane through said smino groups contained on the surface thereof. The DNA fragments chemically bound to the membrane are subjected to hybridization probing with a tagged probe specific to the sequence of the DNA fragments. The tagging may be by either fluorophores or radioisotopes. The tagged probes hybridized to said target DNA fragments are detected and read by laser induced fluorescence detection or autoradiograms. The use of aminated low fluorescent background membranes allows the use of fluorescent detection and reading even when the available amount of DNA to be sequenced is small. The DNA bound to the membrances may be reprobed numerous times.

  7. Challenges and opportunities for structural DNA nanotechnology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In particular, we highlight the potential use of DNA nanostructures in molecular and cellular biophysics, as biomimetic systems, in energy transfer and photonics, and in ...

  8. The Initiation of Bacterial DNA Replication

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The Initiation of Bacterial DNA Replication Print For the first time, scientists have determined the structure of the initiator of bacterial DNA replication. It is already known that such replication is controlled by a protein known as DnaA, a member of the AAA+ superfamily of ATPases. What has now been discovered is that the core of the initiator is not the closed-ring structure expected for this system. Instead, DnaA forms an open right-handed helix. In addition, the architecture indicates

  9. A Route to Scale up DNA Origami Using DNA Tiles as Folding Staples

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Chemie International Edition Year: 2010 Volume: 49 Pages: 1414-1417 ABSTRACT: A new strategy is presented to scale up DNA origami using multi-helical DNA tiles as folding...

  10. SHARED TECHNOLOGY TRANSFER PROGRAM

    SciTech Connect (OSTI)

    GRIFFIN, JOHN M. HAUT, RICHARD C.

    2008-03-07

    The program established a collaborative process with domestic industries for the purpose of sharing Navy-developed technology. Private sector businesses were educated so as to increase their awareness of the vast amount of technologies that are available, with an initial focus on technology applications that are related to the Hydrogen, Fuel Cells and Infrastructure Technologies (Hydrogen) Program of the U.S. Department of Energy. Specifically, the project worked to increase industry awareness of the vast technology resources available to them that have been developed with taxpayer funding. NAVSEA-Carderock and the Houston Advanced Research Center teamed with Nicholls State University to catalog NAVSEA-Carderock unclassified technologies, rated the level of readiness of the technologies and established a web based catalog of the technologies. In particular, the catalog contains technology descriptions, including testing summaries and overviews of related presentations.

  11. Sizing of DNA fragments by flow cytometry

    SciTech Connect (OSTI)

    Johnson, M.E.; Goodwin, P.M.; Ambrose, W.P.; Martin, J.C.; Marrone, B.L.; Jett, J.H.; Keller, R.A.

    1993-02-01

    Individual, stained DNA fragments were sized using a modified flow cytometer with high sensitivity fluorescence detection. The fluorescent intercalating dye ethidium homodimer was used to stain stoichiometrically lambda phage DNA and a Kpn I digest of lambda DNA. Stained, individual fragments of DNA were passed through a low average power, focused, mode-locked laser beam, and the fluorescence from each fragment was collected and quantified. Time-gated detection was used to discriminate against Raman scattering from the water solvent. The fluorescence burst from each fragment was related directly to its length, thus providing a means to size small quantities of kilobase lengths of DNA quickly. Improvements of several orders of magnitude in analysis time and sample size over current gel electrophoresis techniques were realized. Fragments of 17.1,29.9, and 48.5 thousand base pairs were well resolved, and were sized in 164 seconds. Less than one pg of DNA was required for analysis. We have demonstrated sizing of individual, stained DNA fragments with resolution approaching that of gel electrophoresis for moderately large fragments, but with significant reductions in the analysis time and the amount of sample required. Furthermore, system response is linear with DNA fragment length, in contrast to the logarithmic response in gel electrophoresis. There exists the potential to perform this sizing using relatively simple instrumentation, i.e. a continuous wave laser of low power and current mode detection.

  12. Sizing of DNA fragments by flow cytometry

    SciTech Connect (OSTI)

    Johnson, M.E.; Goodwin, P.M.; Ambrose, W.P.; Martin, J.C.; Marrone, B.L.; Jett, J.H.; Keller, R.A.

    1993-01-01

    Individual, stained DNA fragments were sized using a modified flow cytometer with high sensitivity fluorescence detection. The fluorescent intercalating dye ethidium homodimer was used to stain stoichiometrically lambda phage DNA and a Kpn I digest of lambda DNA. Stained, individual fragments of DNA were passed through a low average power, focused, mode-locked laser beam, and the fluorescence from each fragment was collected and quantified. Time-gated detection was used to discriminate against Raman scattering from the water solvent. The fluorescence burst from each fragment was related directly to its length, thus providing a means to size small quantities of kilobase lengths of DNA quickly. Improvements of several orders of magnitude in analysis time and sample size over current gel electrophoresis techniques were realized. Fragments of 17.1,29.9, and 48.5 thousand base pairs were well resolved, and were sized in 164 seconds. Less than one pg of DNA was required for analysis. We have demonstrated sizing of individual, stained DNA fragments with resolution approaching that of gel electrophoresis for moderately large fragments, but with significant reductions in the analysis time and the amount of sample required. Furthermore, system response is linear with DNA fragment length, in contrast to the logarithmic response in gel electrophoresis. There exists the potential to perform this sizing using relatively simple instrumentation, i.e. a continuous wave laser of low power and current mode detection.

  13. Allostery through protein-induced DNA bubbles

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Traverso, Joseph J.; Manoranjan, Valipuram S.; Bishop, A. R.; Rasmussen, Kim Ø.; Voulgarakis, Nikolaos K.

    2015-03-12

    Allostery through DNA is increasingly recognized as an important modulator of DNA functions. Here, we show that the coalescence of protein-induced DNA bubbles can mediate allosteric interactions that drive protein aggregation. We propose that such allostery may regulate DNA's flexibility and the assembly of the transcription machinery. Mitochondrial transcription factor A (TFAM), a dual-function protein involved in mitochondrial DNA (mtDNA) packaging and transcription initiation, is an ideal candidate to test such a hypothesis owing to its ability to locally unwind the double helix. Numerical simulations demonstrate that the coalescence of TFAM-induced bubbles can explain experimentally observed TFAM oligomerization. The resultingmore » melted DNA segment, approximately 10 base pairs long, around the joints of the oligomers act as flexible hinges, which explains the efficiency of TFAM in compacting DNA. Since mitochondrial polymerase (mitoRNAP) is involved in melting the transcription bubble, TFAM may use the same allosteric interaction to both recruit mitoRNAP and initiate transcription.« less

  14. Recombinant DNA encoding a desulfurization biocatalyst

    DOE Patents [OSTI]

    Rambosek, J.; Piddington, C.S.; Kovacevich, B.R.; Young, K.D.; Denome, S.A.

    1994-10-18

    This invention relates to a recombinant DNA molecule containing a gene or genes which encode a biocatalyst capable of desulfurizing a fossil fuel which contains organic sulfur molecules. For example, the present invention encompasses a recombinant DNA molecule containing a gene or genes of a strain of Rhodococcus rhodochrous. 13 figs.

  15. Recombinant DNA encoding a desulfurization biocatalyst

    DOE Patents [OSTI]

    Rambosek, John; Piddington, Chris S.; Kovacevich, Brian R.; Young, Kevin D.; Denome, Sylvia A.

    1994-01-01

    This invention relates to a recombinant DNA molecule containing a gene or genes which encode a biocatalyst capable of desulfurizing a fossil fuel which contains organic sulfur molecules. For example, the present invention encompasses a recombinant DNA molecule containing a gene or genes of a strain of Rhodococcus rhodochrous.

  16. Flow cytometric detection method for DNA samples

    DOE Patents [OSTI]

    Nasarabadi, Shanavaz; Langlois, Richard G.; Venkateswaran, Kodumudi S.

    2006-08-01

    Disclosed herein are two methods for rapid multiplex analysis to determine the presence and identity of target DNA sequences within a DNA sample. Both methods use reporting DNA sequences, e.g., modified conventional Taqman.RTM. probes, to combine multiplex PCR amplification with microsphere-based hybridization using flow cytometry means of detection. Real-time PCR detection can also be incorporated. The first method uses a cyanine dye, such as, Cy3.TM., as the reporter linked to the 5' end of a reporting DNA sequence. The second method positions a reporter dye, e.g., FAM, on the 3' end of the reporting DNA sequence and a quencher dye, e.g., TAMRA, on the 5' end.

  17. Method for sequencing DNA base pairs

    DOE Patents [OSTI]

    Sessler, A.M.; Dawson, J.

    1993-12-14

    The base pairs of a DNA structure are sequenced with the use of a scanning tunneling microscope (STM). The DNA structure is scanned by the STM probe tip, and, as it is being scanned, the DNA structure is separately subjected to a sequence of infrared radiation from four different sources, each source being selected to preferentially excite one of the four different bases in the DNA structure. Each particular base being scanned is subjected to such sequence of infrared radiation from the four different sources as that particular base is being scanned. The DNA structure as a whole is separately imaged for each subjection thereof to radiation from one only of each source. 6 figures.

  18. Flow cytometric detection method for DNA samples

    DOE Patents [OSTI]

    Nasarabadi,Shanavaz; Langlois, Richard G.; Venkateswaran, Kodumudi S.

    2011-07-05

    Disclosed herein are two methods for rapid multiplex analysis to determine the presence and identity of target DNA sequences within a DNA sample. Both methods use reporting DNA sequences, e.g., modified conventional Taqman.RTM. probes, to combine multiplex PCR amplification with microsphere-based hybridization using flow cytometry means of detection. Real-time PCR detection can also be incorporated. The first method uses a cyanine dye, such as, Cy3.TM., as the reporter linked to the 5' end of a reporting DNA sequence. The second method positions a reporter dye, e.g., FAM.TM. on the 3' end of the reporting DNA sequence and a quencher dye, e.g., TAMRA.TM., on the 5' end.

  19. FY04 Engineering Technology Reports Technology Base

    SciTech Connect (OSTI)

    Sharpe, R M

    2005-01-27

    Lawrence Livermore National Laboratory's Engineering Directorate has two primary discretionary avenues for its investment in technologies: the Laboratory Directed Research and Development (LDRD) program and the ''Tech Base'' program. This volume summarizes progress on the projects funded for technology-base efforts in FY2004. The Engineering Technical Reports exemplify Engineering's more than 50-year history of researching and developing (LDRD), and reducing to practice (technology-base) the engineering technologies needed to support the Laboratory's missions. Engineering has been a partner in every major program and project at the Laboratory throughout its existence, and has prepared for this role with a skilled workforce and technical resources. This accomplishment is well summarized by Engineering's mission: ''Enable program success today and ensure the Laboratory's vitality tomorrow''. LDRD is the vehicle for creating those technologies and competencies that are cutting edge. These require a significant level of research or contain some unknown that needs to be fully understood. Tech Base is used to apply those technologies, or adapt them to a Laboratory need. The term commonly used for Tech Base projects is ''reduction to practice''. Tech Base projects effect the natural transition to reduction-to-practice of scientific or engineering methods that are well understood and established. They represent discipline-oriented, core competency activities that are multi-programmatic in application, nature, and scope. The objectives of technology-base funding include: (1) the development and enhancement of tools and processes to provide Engineering support capability, such as code maintenance and improved fabrication methods; (2) support of Engineering science and technology infrastructure, such as the installation or integration of a new capability; (3) support for technical and administrative leadership through our technology Centers; and (4) the initial scoping and

  20. Vehicle Technologies Program Implementation

    SciTech Connect (OSTI)

    none,

    2009-06-19

    The Vehicle Technologies Program takes a systematic approach to Program implementation. Elements of this approach include the evaluation of new technologies, competitive selection of projects and partners, review of Program and project improvement, project tracking, and portfolio management and adjustment.

  1. Tag: technology transfer

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    17all en CNS, UT chemical sensing technology wins R&D 100 Award http:www.y12.doe.govnewspress-releasescns-ut-chemical-sensing-technology-wins-rd-100-award

  2. Technology Transfer Ombudsman Program

    Broader source: Energy.gov [DOE]

    The Technology Transfer Commercialization Act of 2000, Public Law 106-404 (PDF) was enacted in November 2000.  Pursuant to Section 11, Technology Partnerships Ombudsman, each DOE national...

  3. Photovoltaics: A Diverse Technology

    Office of Energy Efficiency and Renewable Energy (EERE)

    This video illustrates the diversity of photovoltaic (PV) technology, which is due to innovations in PV materials, reductions in manufacturing costs, and expanding uses of the technology. A brief...

  4. Technology Readiness Assessment Guide

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2011-09-15

    The Guide assists individuals and teams involved in conducting Technology Readiness Assessments (TRAs) and developing Technology Maturation Plans (TMPs) for the DOE capital asset projects subject to DOE O 413.3B. Supersedes DOE G 413.3-4.

  5. Science & Technology - 2016

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Science & Technology - 2016 February NIF Optics Damage Repair is 2015 Top Technology Pick ... of what takes place when NIF's 192 laser beams enter the pencil eraser-sized gold ...

  6. Technology Deployment Case Studies

    Broader source: Energy.gov [DOE]

    Find technology deployment case studies below. Click on each individual project link to see the full case study. You can also view a map of technology deployment case studies.

  7. SSL TECHNOLOGY DEVELOPMENT WORKSHOP

    Broader source: Energy.gov [DOE]

    Rapid advances in SSL technology make it easy to forget that this technology is still at a relatively early stage of development, and much of its potential remains untapped. The 10th annual DOE SSL...

  8. SSL Technology Development Workshop

    Broader source: Energy.gov [DOE]

    Rapid advances make it easy to forget that SSL technology is still at a relatively early stage of development, and much of its potential remains untapped. The 10th annual DOE SSL Technology...

  9. The shape of the DNA minor groove directs binding by the DNA-bending protein Fis

    SciTech Connect (OSTI)

    Stella, Stefano; Cascio, Duilio; Johnson, Reid C.

    2010-06-21

    The bacterial nucleoid-associated protein Fis regulates diverse reactions by bending DNA and through DNA-dependent interactions with other control proteins and enzymes. In addition to dynamic nonspecific binding to DNA, Fis forms stable complexes with DNA segments that share little sequence conservation. Here we report the first crystal structures of Fis bound to high- and low-affinity 27-base-pair DNA sites. These 11 structures reveal that Fis selects targets primarily through indirect recognition mechanisms involving the shape of the minor groove and sequence-dependent induced fits over adjacent major groove interfaces. The DNA shows an overall curvature of {approx}65{sup o}, and the unprecedented close spacing between helix-turn-helix motifs present in the apodimer is accommodated by severe compression of the central minor groove. In silico DNA structure models show that only the roll, twist, and slide parameters are sufficient to reproduce the changes in minor groove widths and recreate the curved Fis-bound DNA structure. Models based on naked DNA structures suggest that Fis initially selects DNA targets with intrinsically narrow minor grooves using the separation between helix-turn-helix motifs in the Fis dimer as a ruler. Then Fis further compresses the minor groove and bends the DNA to generate the bound structure.

  10. Robert Jilek: Pellion Technologies

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Robert Jilek: Pellion Technologies Alumni Link: Opportunities, News and Resources for Former Employees Latest Issue:September 2015 all issues All Issues » submit Robert Jilek: Pellion Technologies Senior research scientist at eastern energy storage startup September 3, 2014 Robert Jilek Robert Jilek Contact Linda Anderman Email Robert Jilek Jilek is currently with Pellion Technologies Bob Jilek is currently spending part of his time in a management role at Pellion Technologies in the Cambridge

  11. Fuel Cell Technologies Overview

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    7/21/2015 eere.energy.gov Fuel Cell Technologies Overview States Energy Advisory Board (STEAB) Washington, DC Dr. Sunita Satyapal U.S. Department of Energy Fuel Cell Technologies Program Program Manager 3/14/2012 Outline * Introduction - Technology and Market Overview * DOE Program Overview - Mission & Structure - R&D Progress - Demonstration & Deployments * State Activities - Examples of potential opportunities 2 | Fuel Cell Technologies Program Source: US DOE 7/21/2015

  12. Fuel Cell Technologies Overview

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    States Energy Advisory Board (STEAB) Washington, DC Dr. Sunita Satyapal U.S. Department of Energy Fuel Cell Technologies Program Program Manager 3/14/2012 2 | Fuel Cell Technologies Program Source: US DOE 3/19/2013 eere.energy.gov * Introduction - Technology and Market Overview * DOE Program Overview - Mission & Structure - R&D Progress - Demonstration & Deployments * State Activities - Examples of potential opportunities Outline 3 | Fuel Cell Technologies Program Source: US DOE

  13. Geothermal Technologies Program Overview

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Jay Nathwani Acting Program Manager Geothermal Technologies Program Office of Energy Efficiency and Renewable Energy The Geothermal Technologies Program Overview May 18 2010 Energy Efficiency & Renewable Energy eere.energy.gov Geothermal Technologies Program (GTP) Program Topic Areas *Low Temperature, Geopressured and Coproduced Resources *Innovative Exploration Technologies National Goals *Economy Putting people to work in the near- term, and in the future *Security Developing and expanding

  14. Membrane Technology Workshop

    Broader source: Energy.gov [DOE]

    Presentation by Charles Page (Air Products & Chemicals, Inc.) for the Membrane Technology Workshop held July 24, 2012

  15. Benchmarking of Competitive Technologies

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Laboratory * National Renewable Energy Laboratory * ORNL Team Members - Steve Campbell, Chester Coomer - Andy Wereszczak, Materials Science and Technology Division Partners ...

  16. Fuel & Lubricant Technologies

    Broader source: Energy.gov [DOE]

    2012 DOE Hydrogen and Fuel Cells Program and Vehicle Technologies Program Annual Merit Review and Peer Evaluation Meeting

  17. Advanced Propulsion Technology Strategy

    Broader source: Energy.gov [DOE]

    GM is also developing new classes of electrically driven vehicles, leveraging technology first used in their hybrids.

  18. Geothermal Technologies Office April

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Annual Report Geothermal Technologies Office April 2016 1 2015 Annual Report | Geothermal Technologies Office Director's Message Geothermal Technologies Office FY 2016 Budget at a Glance Enhanced Geothermal Systems Hydrothermal Program Low-Temperature and Coproduced Resources Systems Analysis Events and Highlights People Acronyms Resources Table of Contents 2 2 3 7 13 17 19 23 26 28 2015 Achievements Geothermal Technologies Office Steam, West Flank of Coso, NV The 2015 Annual Report of the

  19. Information Technology | Jefferson Lab

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Information Technology The Information Technology Division uses cutting-edge technology to provide high-quality services and capabilities that enable the lab to pursue its research mission in support of the nation's scientific agenda. Leading the division is the chief information officer. The CIO is responsible for providing information from the labs information technology systems to Jefferson Lab management, the overall IT vision, the information architecture for computing and IT, and oversight

  20. Technology Transfer | NREL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Technology Transfer Through partnerships and licensing of its intellectual property rights, NREL seeks to reduce private sector risk in early stage technologies, enable investment in the adoption of renewable energy and energy efficiency technologies, reduce U.S. reliance on foreign energy sources, reduce carbon emissions, and increase U.S. industrial competitiveness. Text Version View a summary of our Fiscal Year 2015 technology partnership agreements. Learn more about our partnership

  1. 1 | Bioenergy Technologies Office

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    2.6.1.2 Analysis for Production - Technical & Market May 20, 2013 Technology Area Review: Biochemical Conversion Sue Jones, Aye Meyer Organization: Pacific Northwest National Laboratory PNNL-SA-95160 This presentation does not contain any proprietary, confidential, or otherwise restricted information 2 | Bioenergy Technologies Office eere.energy.gov Goal Statement * Support the DOE Bioenergy Technologies Office's goal to reduce the estimated mature technology processing cost for converting

  2. Technology Performance Exchange

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... Meeting * Open invitation for peer review 12 | Building Technologies Office ... data flows with Building Component Library * Seamless information flow from ...

  3. TECHNOLOGY READINESS ASSESSMENT

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    DECEMBER 2012 Pathway for readying the next generation of affordable clean energy technology -Carbon Capture, Utilization, and Storage (CCUS) 2012 TECHNOLOGY READINESS ASSESSMENT -OVERVIEW 2 2012 TECHNOLOGY READINESS ASSESSMENT-OVERVIEW 2012 TECHNOLOGY READINESS ASSESSMENT-OVERVIEW 3 DISCLAIMER This report was prepared as an account of work sponsored by an agency of the United States Government. Neither the United States Government nor any agency thereof, nor any of their employees, makes any

  4. Carbon Fiber Technology Facility

    Broader source: Energy.gov [DOE]

    2013 DOE Hydrogen and Fuel Cells Program and Vehicle Technologies Program Annual Merit Review and Peer Evaluation Meeting

  5. Technology Integration Overview

    Broader source: Energy.gov [DOE]

    2013 DOE Hydrogen and Fuel Cells Program and Vehicle Technologies Program Annual Merit Review and Peer Evaluation Meeting

  6. Benchmarking of Competitive Technologies

    Office of Energy Efficiency and Renewable Energy (EERE)

    2012 DOE Hydrogen and Fuel Cells Program and Vehicle Technologies Program Annual Merit Review and Peer Evaluation Meeting

  7. Building Technologies Office Overview

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Roland Risser Director, Building Technologies Office Building Technologies Office Overview Our Homes and Buildings Use 40% of Our Nation's Energy and 75% of Electricity Energy Use Electricity Use Residential Transportation 21 quads 27 quads Commercial 18 quads Industrial 31 quads U.S. Energy Bill for Buildings: $410 billion per year 2 Building Technologies Office (BTO) Ecosystem Emerging Technologies Building Codes Appliance Standards Residential Buildings Integration Commercial Buildings

  8. Consumer Vehicle Technology Data

    Broader source: Energy.gov [DOE]

    2013 DOE Hydrogen and Fuel Cells Program and Vehicle Technologies Program Annual Merit Review and Peer Evaluation Meeting

  9. Geothermal Energy & Drilling Technology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Energy & Drilling Technology - Sandia Energy Energy Search Icon Sandia Home Locations ... Atmospheric Radiation Measurement Climate Reasearch Facility Geomechanics and Drilling ...

  10. Building Technologies Program Presentation

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Renewable Energy Building Technologies Program Jerry Dion Acting Program Manager Building Technologies Program State Energy Advisory Board Meeting October 17, 2007 The investment in Buildings R&D yielded an ROI of 15:1 from 1978 to 2000 The Buildings Technologies Program researches and Energy Efficiency & deploys new technologies to make homes and Renewable Energy commercial buildings more affordable, energy efficient, and better performing The investment in Buildings R&D yielded an

  11. Vehicle Technologies Office

    Broader source: Energy.gov [DOE]

    The Vehicle Technologies Office is developing more energy efficient and environmentally friendly highway transportation technologies that will enable America to use less petroleum. The long-term aim is to develop "leap frog" technologies that will provide Americans with greater freedom of mobility and energy security, while lowering costs and reducing impacts on the environment.

  12. Technology Performance Exchange

    SciTech Connect (OSTI)

    2015-09-01

    To address the need for accessible, high-quality data, the Department of Energy has developed the Technology Performance Exchange (TPEx). TPEx enables technology suppliers, third-party testing laboratories, and other entities to share product performance data. These data are automatically transformed into a format that technology evaluators can easily use in their energy modeling assessments to inform procurement decisions.

  13. High Impact Technology Hub

    Broader source: Energy.gov [DOE]

    The High Impact Technology Hub is a one stop shop for information associated with technology demonstrations in occupied, operational buildings. Resources posted to Hub should accelerate the selection and evaluation of technology demonstration projects and enable transparency into DOEs market stimulation and tech to market activities.

  14. Compression Technology and Needs

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    M ohawk Innovative Technology, Inc. HYDROGEN TRANSMISSION AND DISTRIBUTION WORKSHOP NATIONAL RENEWABLE ENERGY LABORATORY GOLDEN, COLORADO COMPRESSION TECHNOLOGY AND NEEDS Hooshang Heshmat, PH.D. February 25 TH , 2014 ® M ohawk Innovative Technology, Inc. * Overall pipeline delivery steps, production to file up * Different types of compressors * Pipeline compressor development steps and accomplishments * Need for Forecourt Compression system * Other major components: drive, sealing, pipeline,

  15. Vehicle Technologies Office: 2008 Advanced Vehicle Technology...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Use and Greenhouse Gas Emissions of Plug-In Hybrid Electric Vehicles Vehicle Technologies Office: 2010 Vehicle and Systems Simulation and Testing R&D Annual Progress Report

  16. Synthesis of DNA (Patent) | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    Synthesis of DNA Citation Details In-Document Search Title: Synthesis of DNA A method of synthesizing a desired double-stranded DNA of a predetermined length and of a predetermined...

  17. Property:NEPA DNA Worksheet | Open Energy Information

    Open Energy Info (EERE)

    DNA Worksheet Jump to: navigation, search Property Name NEPA DNA Worksheet Property Type Page Description DNA Worksheet files for NEPA Docs. This is a property of type Page. It...

  18. Fuel Cell Technologies Office: Technology Validation Fact Sheet

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    The Fuel Cell Technologies (FCT) Office, through its Technology Validation program, provides a crucial step in the transition of a technology from the lab to commercialization. ...

  19. Annual Report on Technology Transfer and Related Technology Partnering...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Annual Report on Technology Transfer and Related Technology Partnering Activities at the National Laboratories and Other Facilities FY 2009-2013 Annual Report on Technology ...

  20. Vehicle Technologies Office Merit Review 2015: Vehicle Technologies...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Vehicle Technologies Office Overview Vehicle Technologies Office Merit Review 2015: Vehicle Technologies Office Overview Presentation given by U.S. Department of Energy at 2015 DOE ...

  1. Vehicle Technologies Office Merit Review 2014: Carbon Fiber Technology...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Carbon Fiber Technology Facility Vehicle Technologies Office Merit Review 2014: Carbon Fiber Technology Facility Presentation given by Oak Ridge National Laboratory at 2014 DOE ...

  2. Vehicle Technologies Office Merit Review 2015: Advanced Technology...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Advanced Technology Vehicle Lab Benchmarking (L1&L2) Vehicle Technologies Office Merit Review 2015: Advanced Technology Vehicle Lab Benchmarking (L1&L2) Presentation given by Argonne ...

  3. Vehicle Technologies Office: 2008 Oak Ridge Transportation Technology...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Oak Ridge Transportation Technology Program Annual Report Vehicle Technologies Office: 2008 Oak Ridge Transportation Technology Program Annual Report ornlttpreportfy08.pdf (4.58 ...

  4. Gel Electrophoresis of Gold-DNA Nanoconjugates

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Pellegrino, T.; Sperling, R. A.; Alivisatos, A. P.; Parak, W. J.

    2007-01-01

    Gold-DNA conjugates were investigated in detail by a comprehensive gel electrophoresis study based on 1200 gels. A controlled number of single-stranded DNA of different length was attached specifically via thiol-Au bonds to phosphine-stabilized colloidal gold nanoparticles. Alternatively, the surface of the gold particles was saturated with single stranded DNA of different length either specifically via thiol-Au bonds or by nonspecific adsorption. From the experimentally determined electrophoretic mobilities, estimates for the effective diameters of the gold-DNA conjugates were derived by applying two different data treatment approaches. The first method is based on making a calibration curve for the relation between effectivemore » diameters and mobilities with gold nanoparticles of known diameter. The second method is based on Ferguson analysis which uses gold nanoparticles of known diameter as reference database. Our study shows that effective diameters derived from gel electrophoresis measurements are affected with a high error bar as the determined values strongly depend on the method of evaluation, though relative changes in size upon binding of molecules can be detected with high precision. Furthermore, in this study, the specific attachment of DNA via gold-thiol bonds to Au nanoparticles is compared to nonspecific adsorption of DNA. Also, the maximum number of DNA molecules that can be bound per particle was determined.« less

  5. When DNA Needs to Stand Up and Be Counted

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    scientists. Schematic of DNA structures in various conformations on a gold surface. Differences in overall structure and orientation are emphasized by color-coding of DNA...

  6. When DNA Needs to Stand Up and Be Counted

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Understanding both the attachment and orientation of DNA on gold surfaces was the goal of ... Schematic of DNA structures in various conformations on a gold surface. Differences in ...

  7. Microfluidics: Kinetics of Hybridized DNA With Fluid Flow Variations...

    Office of Scientific and Technical Information (OSTI)

    Microfluidics: Kinetics of Hybridized DNA With Fluid Flow Variations. Citation Details In-Document Search Title: Microfluidics: Kinetics of Hybridized DNA With Fluid Flow ...

  8. 6.7 Engineered Enzyme Accelerates DNA Sequencing

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and Richardson, C. C. (1995) "A single residue in DNA polymerases of the Escherichia coli DNA polymerase I family is critical for distinguishing between deoxy- and...

  9. DNA-mediated engineering of multicomponent enzyme crystals (Journal...

    Office of Scientific and Technical Information (OSTI)

    DNA-mediated engineering of multicomponent enzyme crystals Citation Details In-Document Search Title: DNA-mediated engineering of multicomponent enzyme crystals Authors: Brodin, ...

  10. Topoisomerase II Structure Suggests Novel DNA Cleavage Mechanism

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Topoisomerase II Structure Suggests Novel DNA Cleavage Mechanism Print Type II topoisomerases are molecular machines that regulate DNA supercoiling and separate interlocked...

  11. Technology reviews: Shading systems

    SciTech Connect (OSTI)

    Schuman, J.; Rubinstein, F.; Papamichael, K.; Beltran, L.; Lee, E.S.; Selkowitz, S.

    1992-09-01

    We present a representative review of existing, emerging, and future technology options in each of five hardware and systems areas in envelope and lighting technologies: lighting systems, glazing systems, shading systems, daylighting optical systems, and dynamic curtain wall systems. The term technology is used here to describe any design choice for energy efficiency, ranging from individual components to more complex systems to general design strategies. The purpose of this task is to characterize the state of the art in envelope and lighting technologies in order to identify those with promise for advanced integrated systems, with an emphasis on California commercial buildings. For each technology category, the following activities have been attempted to the extent possible: Identify key performance characteristics and criteria for each technology. Determine the performance range of available technologies. Identify the most promising technologies and promising trends in technology advances. Examine market forces and market trends. Develop a continuously growing in-house database to be used throughout the project. A variety of information sources have been used in these technology characterizations, including miscellaneous periodicals, manufacturer catalogs and cut sheets, other research documents, and data from previous computer simulations. We include these different sources in order to best show the type and variety of data available, however publication here does not imply our guarantee of these data. Within each category, several broad classes are identified, and within each class we examine the generic individual technologies that fall into that class.

  12. Method of quantitating dsDNA

    DOE Patents [OSTI]

    Stark, Peter C.; Kuske, Cheryl R.; Mullen, Kenneth I.

    2002-01-01

    A method for quantitating dsDNA in an aqueous sample solution containing an unknown amount of dsDNA. A first aqueous test solution containing a known amount of a fluorescent dye-dsDNA complex and at least one fluorescence-attenutating contaminant is prepared. The fluorescence intensity of the test solution is measured. The first test solution is diluted by a known amount to provide a second test solution having a known concentration of dsDNA. The fluorescence intensity of the second test solution is measured. Additional diluted test solutions are similarly prepared until a sufficiently dilute test solution having a known amount of dsDNA is prepared that has a fluorescence intensity that is not attenuated upon further dilution. The value of the maximum absorbance of this solution between 200-900 nanometers (nm), referred to herein as the threshold absorbance, is measured. A sample solution having an unknown amount of dsDNA and an absorbance identical to that of the sufficiently dilute test solution at the same chosen wavelength is prepared. Dye is then added to the sample solution to form the fluorescent dye-dsDNA-complex, after which the fluorescence intensity of the sample solution is measured and the quantity of dsDNA in the sample solution is determined. Once the threshold absorbance of a sample solution obtained from a particular environment has been determined, any similarly prepared sample solution taken from a similar environment and having the same value for the threshold absorbance can be quantified for dsDNA by adding a large excess of dye to the sample solution and measuring its fluorescence intensity.

  13. An improved DNA force field for ssDNA interactions with gold nanoparticles

    SciTech Connect (OSTI)

    Jiang, Xiankai; Huai, Ping; Fan, Chunhai; Song, Bo E-mail: bosong@sinap.ac.cn; Gao, Jun; Huynh, Tien; Zhou, Ruhong E-mail: bosong@sinap.ac.cn

    2014-06-21

    The widespread applications of single-stranded DNA (ssDNA) conjugated gold nanoparticles (AuNPs) have spurred an increasing interest in the interactions between ssDNA and AuNPs. Despite extensive studies using the most sophisticated experimental techniques, the detailed molecular mechanisms still remain largely unknown. Large scale molecular dynamics (MD) simulations can thus be used to supplement experiments by providing complementary information about ssDNA-AuNP interactions. However, up to now, all modern force fields for DNA were developed based on the properties of double-stranded DNA (dsDNA) molecules, which have hydrophilic outer backbones protecting hydrophobic inner nucleobases from water. Without the double-helix structure of dsDNA and thus the protection by the outer backbone, the nucleobases of ssDNA are directly exposed to solvent, and their behavior in water is very different from that of dsDNA, especially at the interface with nanoparticles. In this work, we have improved the force field of ssDNA for use with nanoparticles, such as AuNPs, based on recent experimental results and quantum mechanics calculations. With the new improved force field, we demonstrated that a poly(A) sequence adsorbed on a AuNP surface is much more stable than a poly(T) sequence, which is consistent with recent experimental observations. On the contrary, the current standard force fields, including AMBER03, CHARMM27, and OPLSAA, all gave erroneous results as compared to experiments. The current improved force field is expected to have wide applications in the study of ssDNA with nanomaterials including AuNPs, which might help promote the development of ssDNA-based biosensors and other bionano-devices.

  14. Konarka Technologies | Open Energy Information

    Open Energy Info (EERE)

    Technologies Jump to: navigation, search Name: Konarka Technologies Place: Lowell, MA Website: www.konarkatechnologies.com References: Konarka Technologies1 Information About...

  15. Gerar Technology | Open Energy Information

    Open Energy Info (EERE)

    Gerar Technology Jump to: navigation, search Name: Gerar Technology Place: Rio de Janeiro, Brazil Product: Developer of new technology for production of biodiesel from vegetable...

  16. EKB Technology | Open Energy Information

    Open Energy Info (EERE)

    EKB Technology Jump to: navigation, search Name: EKB Technology Place: Oxfordshire, United Kingdom Product: Developer of a new bioprocessing technology. Coordinates: 51.813938,...

  17. Rubicon Technology | Open Energy Information

    Open Energy Info (EERE)

    Rubicon Technology Jump to: navigation, search Name: Rubicon Technology Place: Franklin Park, Illinois Zip: 60131 Product: Rubicon Technology makes a sapphire substrates for use in...

  18. Shorepower Technologies | Open Energy Information

    Open Energy Info (EERE)

    Shorepower Technologies Jump to: navigation, search Logo: Shorepower Technologies Name: Shorepower Technologies Address: 2351 NW York St. Place: Portland, Oregon Zip: 97210 Region:...

  19. PCN Technology | Open Energy Information

    Open Energy Info (EERE)

    PCN Technology Jump to: navigation, search Name: PCN Technology Place: San Diego, California Zip: CA 92127 Product: California-based smart grid technology developer. References:...

  20. Topanga Technologies | Open Energy Information

    Open Energy Info (EERE)

    Technologies Place: Canoga Park, California Zip: 91303 Product: Stealth-mode high-intensity discharge (HID) lighting technology developer. References: Topanga Technologies1...

  1. Briza Technologies | Open Energy Information

    Open Energy Info (EERE)

    Briza Technologies Jump to: navigation, search Name: Briza Technologies Place: Hillsborough, New Jersey Zip: 8844 Sector: Wind energy Product: Developing wind turbine technology....

  2. High Impact Technology Hub- Results

    Broader source: Energy.gov [DOE]

    Highlights, outcomes and activities to support the adoption of High Impact Technologies. Technology Highlights preview early results from current technology demonstrations. Case Studies overview...

  3. Minerals Technologies | Open Energy Information

    Open Energy Info (EERE)

    Technologies Jump to: navigation, search Name: Minerals Technologies Place: Bethlehem, PA Website: www.mineralstechnologies.com References: Minerals Technologies1 Information...

  4. DNA fragment sizing and sorting by laser-induced fluorescence

    DOE Patents [OSTI]

    Hammond, Mark L.; Jett, James H.; Keller, Richard A.; Marrone, Babetta L.; Martin, John C.

    1996-01-01

    A method is provided for sizing DNA fragments using high speed detection systems, such as flow cytometry to determine unique characteristics of DNA pieces from a sample. In one characterization the DNA piece is fragmented at preselected sites to produce a plurality of DNA fragments. The DNA piece or the resulting DNA fragments are treated with a dye effective to stain stoichiometrically the DNA piece or the DNA fragments. The fluorescence from the dye in the stained fragments is then examined to generate an output functionally related to the number of nucleotides in each one of the DNA fragments. In one embodiment, the intensity of the fluorescence emissions from each fragment is linearly related to the fragment length. The distribution of DNA fragment sizes forms a characterization of the DNA piece for use in forensic and research applications.

  5. Scar-less multi-part DNA assembly design automation

    DOE Patents [OSTI]

    Hillson, Nathan J.

    2016-06-07

    The present invention provides a method of a method of designing an implementation of a DNA assembly. In an exemplary embodiment, the method includes (1) receiving a list of DNA sequence fragments to be assembled together and an order in which to assemble the DNA sequence fragments, (2) designing DNA oligonucleotides (oligos) for each of the DNA sequence fragments, and (3) creating a plan for adding flanking homology sequences to each of the DNA oligos. In an exemplary embodiment, the method includes (1) receiving a list of DNA sequence fragments to be assembled together and an order in which to assemble the DNA sequence fragments, (2) designing DNA oligonucleotides (oligos) for each of the DNA sequence fragments, and (3) creating a plan for adding optimized overhang sequences to each of the DNA oligos.

  6. Technology transfer 1994

    SciTech Connect (OSTI)

    Not Available

    1994-01-01

    This document, Technology Transfer 94, is intended to communicate that there are many opportunities available to US industry and academic institutions to work with DOE and its laboratories and facilities in the vital activity of improving technology transfer to meet national needs. It has seven major sections: Introduction, Technology Transfer Activities, Access to Laboratories and Facilities, Laboratories and Facilities, DOE Office, Technologies, and an Index. Technology Transfer Activities highlights DOE`s recent developments in technology transfer and describes plans for the future. Access to Laboratories and Facilities describes the many avenues for cooperative interaction between DOE laboratories or facilities and industry, academia, and other government agencies. Laboratories and Facilities profiles the DOE laboratories and facilities involved in technology transfer and presents information on their missions, programs, expertise, facilities, and equipment, along with data on whom to contact for additional information on technology transfer. DOE Offices summarizes the major research and development programs within DOE. It also contains information on how to access DOE scientific and technical information. Technologies provides descriptions of some of the new technologies developed at DOE laboratories and facilities.

  7. Sequential addition of short DNA oligos in DNA-polymerase-based synthesis reactions

    DOE Patents [OSTI]

    Gardner, Shea N; Mariella, Jr., Raymond P; Christian, Allen T; Young, Jennifer A; Clague, David S

    2013-06-25

    A method of preselecting a multiplicity of DNA sequence segments that will comprise the DNA molecule of user-defined sequence, separating the DNA sequence segments temporally, and combining the multiplicity of DNA sequence segments with at least one polymerase enzyme wherein the multiplicity of DNA sequence segments join to produce the DNA molecule of user-defined sequence. Sequence segments may be of length n, where n is an odd integer. In one embodiment the length of desired hybridizing overlap is specified by the user and the sequences and the protocol for combining them are guided by computational (bioinformatics) predictions. In one embodiment sequence segments are combined from multiple reading frames to span the same region of a sequence, so that multiple desired hybridizations may occur with different overlap lengths.

  8. 2010 DOE EERE Vehicle Technologies Program Merit Review … Technology

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Integration | Department of Energy … Technology Integration 2010 DOE EERE Vehicle Technologies Program Merit Review … Technology Integration Technology integration merit review results 2010_amr_08.pdf (989.58 KB) More Documents & Publications 2012 Annual Merit Review Results Report - Technology Integration 2011 Annual Merit Review Results Report - Technology Integration DOE Vehicle Technologies Program 2009 Merit Review Report - Technology Integration and Education

  9. Extracting biological knowledge from DNA sequences

    SciTech Connect (OSTI)

    De La Vega, F.M.; Thieffry, D.; Collado-Vides, J.

    1996-12-31

    This session describes the elucidation of information from dna sequences and what challenges computational biologists face in their task of summarizing and deciphering the human genome. Techniques discussed include methods from statistics, information theory, artificial intelligence and linguistics. 1 ref.

  10. DNA Assembly Line for Nano-Construction

    ScienceCinema (OSTI)

    Oleg Gang

    2010-01-08

    Building on the idea of using DNA to link up nanoparticles scientists at Brookhaven National Lab have designed a molecular assembly line for high-precision nano-construction. Nanofabrication is essential for exploiting the unique properties of nanoparticl

  11. Intriguing DNA Editor Has a Structural Trigger

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Intriguing DNA Editor Has a Structural Trigger Print A powerful new tool for genome editing and gene regulation has emerged in the form of a family of enzymes known as Cas9. Cas9...

  12. Multiple tag labeling method for DNA sequencing

    DOE Patents [OSTI]

    Mathies, R.A.; Huang, X.C.; Quesada, M.A.

    1995-07-25

    A DNA sequencing method is described which uses single lane or channel electrophoresis. Sequencing fragments are separated in the lane and detected using a laser-excited, confocal fluorescence scanner. Each set of DNA sequencing fragments is separated in the same lane and then distinguished using a binary coding scheme employing only two different fluorescent labels. Also described is a method of using radioisotope labels. 5 figs.

  13. Multiple tag labeling method for DNA sequencing

    DOE Patents [OSTI]

    Mathies, Richard A.; Huang, Xiaohua C.; Quesada, Mark A.

    1995-01-01

    A DNA sequencing method described which uses single lane or channel electrophoresis. Sequencing fragments are separated in said lane and detected using a laser-excited, confocal fluorescence scanner. Each set of DNA sequencing fragments is separated in the same lane and then distinguished using a binary coding scheme employing only two different fluorescent labels. Also described is a method of using radio-isotope labels.

  14. Geothermal innovative technologies catalog

    SciTech Connect (OSTI)

    Kenkeremath, D.

    1988-09-01

    The technology items in this report were selected on the basis of technological readiness and applicability to current technology transfer thrusts. The items include technologies that are considered to be within 2 to 3 years of being transferred. While the catalog does not profess to be entirely complete, it does represent an initial attempt at archiving innovative geothermal technologies with ample room for additions as they occur. The catalog itself is divided into five major functional areas: Exploration; Drilling, Well Completion, and Reservoir Production; Materials and Brine Chemistry; Direct Use; and Economics. Within these major divisions are sub-categories identifying specific types of technological advances: Hardware; Software; Data Base; Process/Procedure; Test Facility; and Handbook.

  15. Nuclear Energy Enabling Technologies | Department of Energy

    Energy Savers [EERE]

    Nuclear Energy Enabling Technologies Nuclear Energy Enabling Technologies The Nuclear Energy Enabling Technologies (NEET) Program will develop crosscutting technologies that ...

  16. Environmental Technology Verification of Mobile Sources Control...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Environmental Technology Verification of Mobile Sources Control Technologies Environmental Technology Verification of Mobile Sources Control Technologies 2005 Diesel Engine...

  17. Geothermal Technologies Office - Webmaster | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    - Webmaster Geothermal Technologies Office - Webmaster

  18. Bioenergy Technologies Presentation

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Technologies State Energy Advisory Board Meeting October 18, 2007 ORNL Jonathan R, Mielenz ORNL Biomass Program Manager & Bioconversion Science and Technology President's State of the Union Address January 2006 Keeping America competitive requires affordable energy. And here we have a serious problem: America is addicted to oil, which is often imported from unstable parts of the world. The best way to break this addiction is through technology.... and we are on the threshold of incredible

  19. Summary - Caustic Recovery Technology

    Office of Environmental Management (EM)

    Caustic Recovery Technology ETR Report Date: July 2007 ETR-7 United States Department of Energy Office of Environmental Management (DOE-EM) External Technical Review of Caustic Recovery Technology Why DOE-EM Did This Review The Department of Energy (DOE) Environmental Management Office (EM-21) has been developing caustic recovery technology for application to the Hanford Waste Treatment Plant (WTP) to reduce the amount of Low Activity Waste (LAW) vitrified. Recycle of sodium hydroxide with an

  20. Information Sciences and Technology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Information Sciences and Technology Information Sciences and Technology National security depends on science and technology. The United States relies on Los Alamos National Laboratory for the best of both. No place on Earth pursues a broader array of world-class scientific endeavors. Contact thumbnail of Business Development Executive Steve Stringer Business Development Executive Richard P. Feynman Center for Innovation (505) 660-2177 Email Los Alamos leverages advances in theory, algorithms,

  1. Technologies | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Technologies Available for Licensing Energy Storage Industrial & Manufacturing Processes Licensable Software Life Sciences Materials Transportation Fact Sheets and Forms Licensable Technologies Argonne's researchers have developed a wide and diverse range of technologies that have worldwide impact in a variety of fields. Argonne grants licenses for lab-developed intellectual property to existing and start-up companies that are technically and financially capable of turning early-stage

  2. 2013 DOE Bioenergy Technologies

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Bioenergy Technologies Office (BETO) Project Peer Review Catalytic Conversion of Lignocellulosic Biomass to Hydrocarbon Fuels (2 3 1 12) Technology Area Review: Biochemical Conversion 1 | Bioenergy Technologies Office eere.energy.gov Hydrocarbon Fuels (2.3.1.12) May 22, 2013 Mike Lilga This presentation does not contain any proprietary, confidential, or otherwise restricted information Conversion Organization: PNNL Goal Statement Goals: * There is a need to make a balanced f el composition

  3. Technology Pathway Selection Effort

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    BIOMASS PROGRAM Technology Pathway Selection Effort Alicia Lindauer 27 November 2012 2 | Biomass Program eere.energy.gov * Setting R&D priorities * Benchmarking * Informing multi-sectoral analytical activities * Track Program R&D progress against goals * Identify technology process routes and prioritize funding * Program direction decisions: * Are we spending our money on the right technology pathways? * Within a pathway: Are we focusing our funding on the highest priority activities?

  4. TECHNOLOGY READINESS ASSESSMENT

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ASSESSMENT JANUARY 2015 -A CHECKPOINT ALONG A CHALLENGING JOURNEY DOE/NETL-2015/1710 U.S. Department of Energy 2014 TECHNOLOGY READINESS ASSESSMENT-CLEAN COAL RESEARCH PROGRAM 2 2014 TECHNOLOGY READINESS ASSESSMENT-CLEAN COAL RESEARCH PROGRAM Office of Fossil Energy | National Energy Technology Laboratory DISCLAIMER 3 DISCLAIMER This report was prepared as an account of work sponsored by an agency of the United States Government. Neither the United States Government nor any agency thereof, nor

  5. Technology Transfer - JCAP

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    PAZ0004_v2.jpg Technology Transfer Who We Are JCAP Mission JCAP At A Glance Fact Sheets Organizational Chart Recent Science Technology Transfer Awards & Honors Senior Management Scientific Leadership Researchers Governance & Advisory Boards Operations & Administration Who we are Overview JCAP Mission JCAP At A Glance Fact Sheets Organizational Chart Our Achievements Recent Science Technology Transfer Awards & Honors Our People Senior Management Scientific Leadership Researchers

  6. Digital Sensor Technology

    SciTech Connect (OSTI)

    Ted Quinn; Jerry Mauck; Richard Bockhorst; Ken Thomas

    2013-07-01

    The nuclear industry has been slow to incorporate digital sensor technology into nuclear plant designs due to concerns with digital qualification issues. However, the benefits of digital sensor technology for nuclear plant instrumentation are substantial in terms of accuracy, reliability, availability, and maintainability. This report demonstrates these benefits in direct comparisons of digital and analog sensor applications. It also addresses the qualification issues that must be addressed in the application of digital sensor technology.

  7. Carbon Fiber Technology Facility

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Carbon Fiber Technology Facility Dave Warren, PI Cliff Eberle, Presenter Technology Development Manager Polymer Matrix Composites Oak Ridge National Laboratory May 16, 2012 Project ID # LM003 Status as of March 30, 2012 This presentation does not contain any proprietary, confidential, or otherwise restricted information 2 Managed by UT-Battelle for the U.S. Department of Energy Carbon Fiber Technology Facility (CFTF) ARRA CAPITAL Project Overview * Funds received FY10Q2 * Scheduled finish FY13Q4

  8. Oligonucleotide and Long Polymeric DNA Encoding

    SciTech Connect (OSTI)

    Miller, E; Mariella Jr., R P; Christian, A T; Gardner, S N; Williams, J M

    2003-11-24

    This report summarizes the work done at Lawrence Livermore National Laboratory for the Oligonucleotide and Long Polymeric DNA Encoding project, part of the Microelectronic Bioprocesses Program at DARPA. The goal of the project was to develop a process by which long (circa 10,000 base-pair) synthetic DNA molecules could be synthesized in a timely and economic manner. During construction of the long molecule, errors in DNA sequence occur during hybridization and/or the subsequent enzymatic process. The work done on this project has resulted in a novel synthesis scheme that we call the parallel pyramid synthesis protocol, the development of a suit of computational tools to minimize and quantify errors in the synthesized DNA sequence, and experimental proof of this technique. The modeling consists of three interrelated modules: the bioinformatics code which determines the specifics of parallel pyramid synthesis for a given chain of long DNA, the thermodynamics code which tracks the products of DNA hybridization and polymerase extension during the later steps in the process, and the kinetics model which examines the temporal and spatial processes during one thermocycle. Most importantly, we conducted the first successful syntheses of a gene using small starting oligomers (tetramers). The synthesized sequence, 813 base pairs long, contained a 725 base pair gene, modified green fluorescent protein (mGFP), which has been shown to be a functional gene by cloning into cells and observing its green fluorescent product.

  9. Mechanism of homologous recombination from the RecA-ssDNA/dsDNA...

    Office of Scientific and Technical Information (OSTI)

    Sponsoring Org: USDOE Country of Publication: United States Language: ENGLISH Subject: 60 APPLIED LIFE SCIENCES; ATP-ASE; ATP; CRYSTAL STRUCTURE; DNA; ESCHERICHIA COLI; FILAMENTS; ...

  10. Next generation sequencing (NGS)technologies and applications

    SciTech Connect (OSTI)

    Vuyisich, Momchilo

    2012-09-11

    NGS technology overview: (1) NGS library preparation - Nucleic acids extraction, Sample quality control, RNA conversion to cDNA, Addition of sequencing adapters, Quality control of library; (2) Sequencing - Clonal amplification of library fragments, (except PacBio), Sequencing by synthesis, Data output (reads and quality); and (3) Data analysis - Read mapping, Genome assembly, Gene expression, Operon structure, sRNA discovery, and Epigenetic analyses.

  11. Information Sciences and Technology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    file systems Bioinformatics Infectious disease surveillance Climate change and energy security Smart grids Learn more about our Information Science and Technology capabilities

  12. Fuel Cell Technologies Budget

    SciTech Connect (OSTI)

    EERE

    2012-03-16

    The Fuel Cell Technologies Office receives appropriations from Energy and Water Development. The offices's major activities and budget are outlined in this Web page.

  13. Supervisory Information Technology Specialist

    Broader source: Energy.gov [DOE]

    A successful candidate in this position will be responsible for providing Information Technology (IT) infrastructure, capabilities and technical support to the Department of Energy (DOE),...

  14. Emerging Technologies (ET)

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... Appliance and Equipment Standards * ENERGY STAR Theory of Impact: Deployment programs utilize reports as technology baseline. Theory of Impact: Supports performance and cost ...

  15. Technology Demonstration Partnership Policy

    Broader source: Energy.gov [DOE]

    This City Council memorandum establishes a framework for engaging in and evaluating demonstration partnerships with the goal of developing, testing, and demonstrating emerging technologies, product, and service innovations.

  16. Sandia Science & Technology Park

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Laboratories. More Info Liquid Common SS&TP welcomes Liquid Common Liquid Common is a digital marketing company now located in the Park. More Info Sandia Science & Technology...

  17. Information Technology Specialist (Security)

    Broader source: Energy.gov [DOE]

    A successful candidate in this position will serve as an Information Technology Specialist (Security) responsible for providing technical support in the information security environment which...

  18. Director, Geothermal Technologies Office

    Broader source: Energy.gov [DOE]

    The mission of the Geothermal Technologies Office (GTO) is to accelerate the development and deployment of clean, domestic geothermal resources that will promote a stronger, more productive economy...

  19. Information Science & Technology Institute

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ISTI Information Science & Technology Institute Providing connection to program management for capability needs, as well as IS&T integration and support for mission-critical...

  20. Technology Integration Overview

    Broader source: Energy.gov [DOE]

    2010 DOE Vehicle Technologies and Hydrogen Programs Annual Merit Review and Peer Evaluation Meeting, June 7-11, 2010 -- Washington D.C.

  1. Appendix C - Industrial technologies

    SciTech Connect (OSTI)

    None, None

    2002-12-20

    This report describes the results, calculations, and assumptions underlying the GPRA 2004 Quality Metrics results for all Planning Units within the Office of Industrial Technologies.

  2. Window Industry Technology Roadmap

    SciTech Connect (OSTI)

    None, None

    2000-04-01

    The Window Industry Technology Roadmap looks at the trends in window design and installation in 2000 and projects trends for the future.

  3. Solar Energy Technologies Office

    Office of Energy Efficiency and Renewable Energy (EERE)

    In 2011, the Energy Department's Solar Energy Technologies Office (SETO) became the SunShot Initiative, a collaborative national effort that aggressively drives innovation to make solar energy...

  4. Benchmarking of Competitive Technologies

    Broader source: Energy.gov [DOE]

    2010 DOE Vehicle Technologies and Hydrogen Programs Annual Merit Review and Peer Evaluation Meeting, June 7-11, 2010 -- Washington D.C.

  5. ocean energy technologies

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... Tribal Energy Program Intellectual Property Current EC Partnerships How to Partner Small ... SunShot Grand Challenge: Regional Test Centers ocean energy technologies HomeTag:ocean ...

  6. Overview of geothermal technologies

    SciTech Connect (OSTI)

    None, None

    2009-01-18

    The geothermal overview section of the Renewable Energy Technology Characterizations describes the technical and economic status of this emerging renewable energy option for electricity supply.

  7. Information Technology Specialist

    Broader source: Energy.gov [DOE]

    The Information Technology Services Office (ITSO) is an office within the Office of Business Operations (BO) that provides administrative support to the Office of Energy Efficiency and Renewable...

  8. Benchmarking of Competitive Technologies

    Broader source: Energy.gov [DOE]

    2009 DOE Hydrogen Program and Vehicle Technologies Program Annual Merit Review and Peer Evaluation Meeting, May 18-22, 2009 -- Washington D.C.

  9. ENERGY EFFICIENCY TECHNOLOGY ROADMAP

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    managed the overall development and maturation of this Energy Efficiency Technology Roadmap, the effort would not have been possible without the active engagement of a diverse...

  10. Collaborative Transmission Technology Roadmap

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Addendum to the Collaborative Transmission Technology Roadmap March 2014 Bonneville Power Administration Enhanced PDF Functionality Functionality of the PDF version of this...

  11. Renewable energy technology characterizations

    SciTech Connect (OSTI)

    None, None

    1997-12-01

    The Renewable Energy Technology Characterizations describe the technical and economic status of the major emerging renewable energy options for electricity supply.

  12. Sorption Storage Technology Summary

    Broader source: Energy.gov [DOE]

    Presented at the R&D Strategies for Compressed, Cryo-Compressed and Cryo-Sorbent Hydrogen Storage Technologies Workshops on February 14 and 15, 2011.

  13. New and Emerging Technologies

    Office of Energy Efficiency and Renewable Energy (EERE)

    This power point presentation provides an overview of CHP technologies and how they can be used in industrial manufacturing plants to increase productivity and reduce energy and costs.

  14. Energy Technology Program Specialist

    Broader source: Energy.gov [DOE]

    The Office of Energy Efficiency and Renewable Energy is the lead Federal government organization for energy efficiency and renewable energy technology research and development. Its mission is to...

  15. Overview of wind technologies

    SciTech Connect (OSTI)

    None, None

    2009-01-18

    The wind overview section of the Renewable Energy Technology Characterizations describes the technical and economic status of this emerging renewable energy option for electricity supply.

  16. Overview of biomass technologies

    SciTech Connect (OSTI)

    None, None

    2009-01-18

    The biomass overview of the Renewable Energy Technology Characterizations describes the technical and economic status of this emerging renewable energy option for electricity supply.

  17. Mobile Technology Management

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2013-11-21

    The directive will ensure that federal organizations and employees within the Department can use mobile technology to support mission requirements in a safe and secure manner.

  18. NREL Commercialization & Technology Transfer

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... The NREL Industry Growth Forum accelerates the commercialization of clean energy technologies by: * Fostering hands-on-management and coaching for evolving clean energy companies * ...

  19. Massachusetts Institute of Technology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of Technology Short baseline neutrino workshop, Fermilab, Batavia, IL, May 13, 2011 Test of Lorentz and CPT violation with neutrinos Outline 1. Why Lorentz violation is...

  20. Science & Technology Review Articles

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    NIF & Photon Science News Press Releases Experimental Highlights Efficiency Improvements Science & Technology Meetings and Workshops Papers and Presentations NIF&PS People In the ...

  1. Wind Energy Technology Basics

    K-12 Energy Lesson Plans and Activities Web site (EERE)

    Wind energy technologies use the energy in wind for practical purposes such as generating electricity, charging batteries, pumping water, and grinding grain.

  2. Technology Readiness Assessment Report

    Office of Environmental Management (EM)

    ... was pioneered by the National Aeronautics and Space Administration (NASA) in the 1980s. ... for technology insertion into design by NASA. In March of 2007, the GAO recommended ...

  3. 2016 Technology Innovation Projects

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Projects FY 2016 Technology Innovation Project Briefs Demand Response TIP 292: Advanced Heat Pump Water Heater Research TIP 336: Scaled Deployment and Demonstration of Demand...

  4. SRNL LDRD - Developed Technologies

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Developed Technologies Porous Wall Hollow Glass Microspheres Porous Wall Hollow Glass Microspheres Tiny Glass Spheres for Energy Storage, Medical Applications and Other Uses...

  5. Advanced Optical Technologies

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The Advanced Optical Components and Technologies program develops, creates and provides critical optical components for laser-based missions at LLNL. Past projects focused on ...

  6. Consumer Vehicle Technology Data

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    technologies Relevance: An informed understanding of the consumer allows VTO to achieve petroleum-use reduction goals through: * Robust assumptions for consumer modeling,...

  7. Consumer Vehicle Technology Data

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    technologies. Relevance: An informed understanding of the consumer allows VTO to achieve petroleum-use reduction goals through: * Robust assumptions for consumer modeling,...

  8. Ocean Energy Technology Overview

    SciTech Connect (OSTI)

    none,

    2009-08-05

    Introduction to and overview of ocean renewable energy resources and technologies prepared for the U.S. Department of Energy Federal Energy management Program.

  9. Marine & Hydrokinetic Technologies

    SciTech Connect (OSTI)

    2011-07-01

    This fact sheet describes the Wind and Water Power Program's current approach to supporting the development and deployment of marine and hydrokinetic technologies.

  10. A Green Technology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Green Technology - Sandia Energy Energy Search Icon Sandia Home Locations Contact Us Employee Locator Energy & Climate Secure & Sustainable Energy Future Stationary Power Energy ...

  11. Algal Biofuel Technologies

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Algal Biofuel Technologies States Biomass Clean Cities Web Conference November 6, 2008 Al Darzins, Ph.D. Principal Group Manager National Bioenergy Center NREL is a national ...

  12. Recycling Technology Validation

    Broader source: Energy.gov [DOE]

    2009 DOE Hydrogen Program and Vehicle Technologies Program Annual Merit Review and Peer Evaluation Meeting, May 18-22, 2009 -- Washington D.C.

  13. Hydrocarbon Technologies | Open Energy Information

    Open Energy Info (EERE)

    Technologies Jump to: navigation, search Name: Hydrocarbon Technologies Place: Lawrenceville, New Jersey Zip: 8648 Sector: Efficiency Product: String representation...

  14. Bioconversion Technologies | Open Energy Information

    Open Energy Info (EERE)

    to: navigation, search Name: Bioconversion Technologies Place: United Kingdom Sector: Biofuels Product: Second-generation biofuels technology developer References: Bioconversion...

  15. Emerging Technologies - Capturing Innovation with Technology

    SciTech Connect (OSTI)

    2012-12-01

    ET team research results are critical to achieving 50% energy savings across U.S. buildings within the next two decades. The ET team focuses on supporting research, development, and tech-to-market opportunities of high impact technologies, or those that demonstrate potential for achieving significant energy savings cost effectively.

  16. Robotics Technology Crosscutting Program. Technology summary

    SciTech Connect (OSTI)

    1995-06-01

    The Robotics Technology Development Program (RTDP) is a needs-driven effort. A length series of presentations and discussions at DOE sites considered critical to DOE`s Environmental Restoration and Waste Management (EM) Programs resulted in a clear understanding of needed robotics applications toward resolving definitive problems at the sites. A detailed analysis of the resulting robotics needs assessment revealed several common threads running through the sites: Tank Waste Retrieval (TWR), Contaminant Analysis Automation (CAA), Mixed Waste Operations (MWO), and Decontamination and Dismantlement (D and D). The RTDP Group also realized that some of the technology development in these four areas had common (Cross Cutting-CC) needs, for example, computer control and sensor interface protocols. Further, the OTD approach to the Research, Development, Demonstration, Testing, and Evaluation (RDDT and E) process urged an additional organizational breakdown between short-term (1--3 years) and long-term (3--5 years) efforts (Advanced Technology-AT). These factors lead to the formation of the fifth application area for Crosscutting and Advanced Technology (CC and AT) development. The RTDP is thus organized around these application areas -- TWR, CAA, MWO, D and D, and CC and AT -- with the first four developing short-term applied robotics. An RTDP Five-Year Plan was developed for organizing the Program to meet the needs in these application areas.

  17. Assistive Technology | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Assistive Technology Assistive Technology Assistive technology word cloud. Assistive technology word cloud. The DOE Headquarters Accommodation Program was established to provide reasonable computer and related telecommunications accommodations for employees with disabilities. Since implementation of the Assistive Technologies program in 1993, accommodations have increased from an initial 26 to an approximately 700 individual accommodations. The Assistive Technologies program complies with

  18. Digital Actuator Technology

    SciTech Connect (OSTI)

    Ken Thomas; Ted Quinn; Jerry Mauck; Richard Bockhorst

    2014-09-01

    There are significant developments underway in new types of actuators for power plant active components. Many of these make use of digital technology to provide a wide array of benefits in performance of the actuators and in reduced burden to maintain them. These new product offerings have gained considerable acceptance in use in process plants. In addition, they have been used in conventional power generation very successfully. This technology has been proven to deliver the benefits promised and substantiate the claims of improved performance. The nuclear industry has been reluctant to incorporate digital actuator technology into nuclear plant designs due to concerns due to a number of concerns. These could be summarized as cost, regulatory uncertainty, and a certain comfort factor with legacy analog technology. The replacement opportunity for these types of components represents a decision point for whether to invest in more modern technology that would provide superior operational and maintenance benefits. Yet, the application of digital technology has been problematic for the nuclear industry, due to qualification and regulatory issues. With some notable exceptions, the result has been a continuing reluctance to undertake the risks and uncertainties of implementing digital actuator technology when replacement opportunities present themselves. Rather, utilities would typically prefer to accept the performance limitations of the legacy analog actuator technologies to avoid impacts to project costs and schedules. The purpose of this report is to demonstrate that the benefits of digital actuator technology can be significant in terms of plant performance and that it is worthwhile to address the barriers currently holding back the widespread development and use of this technology. It addresses two important objectives in pursuit of the beneficial use of digital actuator technology for nuclear power plants: 1. To demonstrate the benefits of digital actuator

  19. Technology Catalogue. First edition

    SciTech Connect (OSTI)

    Not Available

    1994-02-01

    The Department of Energy`s Office of Environmental Restoration and Waste Management (EM) is responsible for remediating its contaminated sites and managing its waste inventory in a safe and efficient manner. EM`s Office of Technology Development (OTD) supports applied research and demonstration efforts to develop and transfer innovative, cost-effective technologies to its site clean-up and waste management programs within EM`s Office of Environmental Restoration and Office of Waste Management. The purpose of the Technology Catalogue is to provide performance data on OTD-developed technologies to scientists and engineers assessing and recommending technical solutions within the Department`s clean-up and waste management programs, as well as to industry, other federal and state agencies, and the academic community. OTD`s applied research and demonstration activities are conducted in programs referred to as Integrated Demonstrations (IDs) and Integrated Programs (IPs). The IDs test and evaluate.systems, consisting of coupled technologies, at specific sites to address generic problems, such as the sensing, treatment, and disposal of buried waste containers. The IPs support applied research activities in specific applications areas, such as in situ remediation, efficient separations processes, and site characterization. The Technology Catalogue is a means for communicating the status. of the development of these innovative technologies. The FY93 Technology Catalogue features technologies successfully demonstrated in the field through IDs and sufficiently mature to be used in the near-term. Technologies from the following IDs are featured in the FY93 Technology Catalogue: Buried Waste ID (Idaho National Engineering Laboratory, Idaho); Mixed Waste Landfill ID (Sandia National Laboratories, New Mexico); Underground Storage Tank ID (Hanford, Washington); Volatile organic compound (VOC) Arid ID (Richland, Washington); and VOC Non-Arid ID (Savannah River Site, South Carolina).

  20. Additive Manufacturing Technology Assessment

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Additive Manufacturing 1 Technology Assessment 2 1. Contents 3 1. Introduction to the Technology/System ............................................................................................... 2 4 1.1 Introduction to Additive Manufacturing ....................................................................................... 2 5 1.2 Additive Manufacturing Processes ............................................................................................... 2 6 1.3 Benefits of Additive

  1. Gasification: A Cornerstone Technology

    SciTech Connect (OSTI)

    Gary Stiegel

    2008-03-26

    NETL is a leader in the science and technology of gasification - a process for the conversion of carbon-based materials such as coal into synthesis gas (syngas) that can be used to produce clean electrical energy, transportation fuels, and chemicals efficiently and cost-effectively using domestic fuel resources. Gasification is a cornerstone technology of 21st century zero emissions powerplants

  2. Gasification: A Cornerstone Technology

    ScienceCinema (OSTI)

    Gary Stiegel

    2010-01-08

    NETL is a leader in the science and technology of gasification - a process for the conversion of carbon-based materials such as coal into synthesis gas (syngas) that can be used to produce clean electrical energy, transportation fuels, and chemicals efficiently and cost-effectively using domestic fuel resources. Gasification is a cornerstone technology of 21st century zero emissions powerplants

  3. Advanced uranium enrichment technologies

    SciTech Connect (OSTI)

    Merriman, R.

    1983-03-10

    The Advanced Gas Centrifuge and Atomic Vapor Laser Isotope Separation methods are described. The status and potential of the technologies are summarized, the programs outlined, and the economic incentives are noted. How the advanced technologies, once demonstrated, might be deployed so that SWV costs in the 1990s can be significantly reduced is described.

  4. Technology Readiness Assessment Guide

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2011-09-15

    This document was developed to assist individuals and teams that will be involved in conducting Technology Readiness Assessments (TRAs) and developing Technology Maturation Plans (TMPs) for the Department of Energy (DOE) capital acquisition assets subjects to DOE O 413.3B.

  5. Accelerating Spectrum Sharing Technologies

    SciTech Connect (OSTI)

    Juan D. Deaton; Lynda L. Brighton; Rangam Subramanian; Hussein Moradi; Jose Loera

    2013-09-01

    Spectrum sharing potentially holds the promise of solving the emerging spectrum crisis. However, technology innovators face the conundrum of developing spectrum sharing technologies without the ability to experiment and test with real incumbent systems. Interference with operational incumbents can prevent critical services, and the cost of deploying and operating an incumbent system can be prohibitive. Thus, the lack of incumbent systems and frequency authorization for technology incubation and demonstration has stymied spectrum sharing research. To this end, industry, academia, and regulators all require a test facility for validating hypotheses and demonstrating functionality without affecting operational incumbent systems. This article proposes a four-phase program supported by our spectrum accountability architecture. We propose that our comprehensive experimentation and testing approach for technology incubation and demonstration will accelerate the development of spectrum sharing technologies.

  6. Technology Readiness Assessment (TRA)/Technology Maturation Plan (TMP)

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Process Guide | Department of Energy Readiness Assessment (TRA)/Technology Maturation Plan (TMP) Process Guide Technology Readiness Assessment (TRA)/Technology Maturation Plan (TMP) Process Guide This document is a guide for those involved in conducting TRAs and developing TMPs for DOE-EM. Technology Readiness Assessment (TRA)/Technology Maturation Plan (TMP) Process Guide (1.19 MB) More Documents & Publications Technology Readiness Assessment Report Small Column Ion Exchange Technology

  7. DOE Vehicle Technologies Program 2009 Merit Review Report - Technology

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Validation | Department of Energy Technology Validation DOE Vehicle Technologies Program 2009 Merit Review Report - Technology Validation Merit review of DOE Vehicle Technologies Program research efforts 2009_merit_review_9.pdf (454.83 KB) More Documents & Publications DOE Vehicle Technologies Program 2009 Merit Review Report DOE Vehicle Technologies Program 2009 Merit Review Report - Vehicle Systems DOE Vehicle Technologies Program 2009 Merit Review Report - Safety Codes and Standard

  8. DOE Vehicle Technologies Program 2009 Merit Review Report - Technology

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Integration and Education | Department of Energy Technology Integration and Education DOE Vehicle Technologies Program 2009 Merit Review Report - Technology Integration and Education Merit review of DOE Vehicle Technologies Program research efforts 2009_merit_review_8.pdf (931.92 KB) More Documents & Publications DOE Vehicle Technologies Program 2009 Merit Review Report - Technology Validation DOE Vehicle Technologies Program 2009 Merit Review Report - Lightweight Materials 2008 Annual

  9. DNA Persistence in Sink Drain Environment

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Winder, Eric M.; Bonheyo, George T.

    2015-07-31

    Biofilms are organized structures composed mainly of cells and extracellular polymeric substances produced by the constituent microorganisms. Ubiquitous in nature, biofilms have an innate ability to capture and retain passing material and may therefore act as natural collectors of contaminants or signatures of upstream activities. To determine the persistence and detectability of DNA passing through a sink drain environment, Bacillus anthracis strain Ames35 was cultured (6.35 x 107 CFU/mL), sterilized, and disposed of by addition to a sink drain apparatus with an established biofilm. The sink drain apparatus was sampled before and for several days after the addition of themore » sterilized B. anthracis culture to detect the presence of B. anthracis DNA. Multiple PCR primer pairs were used to screen for chromosomal and plasmid DNA with primers targeting shorter sequences showing greater amplification efficiency and success. PCR amplification and detection of target sequences indicate persistence of chromosomal DNA and plasmid DNA in the biofilm for 5 or more and 14 or more days, respectively.« less

  10. Optical selection and collection of DNA fragments

    DOE Patents [OSTI]

    Roslaniec, Mary C.; Martin, John C.; Jett, James H.; Cram, L. Scott

    1998-01-01

    Optical selection and collection of DNA fragments. The present invention includes the optical selection and collection of large (>.mu.g) quantities of clonable, chromosome-specific DNA from a sample of chromosomes. Chromosome selection is based on selective, irreversible photoinactivation of unwanted chromosomal DNA. Although more general procedures may be envisioned, the invention is demonstrated by processing chromosomes in a conventional flow cytometry apparatus, but where no droplets are generated. All chromosomes in the sample are first stained with at least one fluorescent analytic dye and bonded to a photochemically active species which can render chromosomal DNA unclonable if activated. After passing through analyzing light beam(s), unwanted chromosomes are irradiated using light which is absorbed by the photochemically active species, thereby causing photoinactivation. As desired chromosomes pass this photoinactivation point, the inactivating light source is deflected by an optical modulator; hence, desired chromosomes are not photoinactivated and remain clonable. The selection and photoinactivation processes take place on a microsecond timescale. By eliminating droplet formation, chromosome selection rates 50 times greater than those possible with conventional chromosome sorters may be obtained. Thus, usable quantities of clonable DNA from any source thereof may be collected.

  11. Robotics Technology Development Program. Technology summary

    SciTech Connect (OSTI)

    Not Available

    1994-02-01

    The Robotics Technology Development Program (RTDP) is a ``needs-driven`` effort. A lengthy series of presentations and discussions at DOE sites considered critical to DOE`s Environmental Restoration and Waste Management (EM) Programs resulted in a clear understanding of needed robotics applications toward resolving definitive problems at the sites. A detailed analysis of the Tank Waste Retrieval (TWR), Contaminant Analysis Automation (CAA), Mixed Waste Operations (MWO), and Decontamination & Dismantlement (D&D). The RTDP Group realized that much of the technology development was common (Cross Cutting-CC) to each of these robotics application areas, for example, computer control and sensor interface protocols. Further, the OTD approach to the Research, Development, Demonstration, Testing, and Evaluation (RDDT&E) process urged an additional organizational break-out between short-term (1--3 years) and long-term (3--5 years) efforts (Advanced Technology-AT). The RDTP is thus organized around these application areas -- TWR, CAA, MWO, D&D and CC&AT -- with the first four developing short-term applied robotics. An RTDP Five-Year Plan was developed for organizing the Program to meet the needs in these application areas.

  12. Taking technology to market

    SciTech Connect (OSTI)

    Ford, D.; Ryan, C.

    1981-03-01

    For many years, the concept of the product life cycle has helped managers maximize their return on product sales. But according to the authors of this article, using a technology solely in product sales is no longer enough. Today, companies face high R and D costs, competitive pressures from low-cost producers, capacity limitations, antitrust laws, financial difficulties, and foreign trade barriers. This means that they must improve the rate of return on their technology investments by marketing their technology as completely as possible during all phases of its life cycle. The technology life cycle - derived from the product life cycle - pinpoints the changing decisions companies face in selling their know-how. The authors also discuss both the competitive dangers of transferring technology to low-cost foreign producers and the growing role of intermediaries in technology sales. They stress the importance of having a highly specialized staff to plan a company's technology marketing, a responsibility that should be assigned neither to the part-time attention of top management nor simply to marketers or strategic planners.

  13. Annual Technology Baseline

    Broader source: Energy.gov [DOE]

    The National Renewable Energy Laboratory is conducting a study sponsored by the U.S. Department of Energy DOE, Office of Energy Efficiency and Renewable Energy (EERE), that aims to document and implement an annual process designed to identify a realistic and timely set of input assumptions (e.g., technology cost and performance, fuel costs), and a diverse set of potential futures (standard scenarios), initially for electric sector analysis. This primary product of the Annual Technology Baseline (ATB) project component includes detailed cost and performance data (both current and projected) for both renewable and conventional technologies. This data is presented in MS Excel.

  14. Agricultural Equipment Technology Conference

    Broader source: Energy.gov [DOE]

    The 20th Agricultural Equipment Technology Conference will be held Feb. 8–10, 2016, in Louisville, Kentucky. The conference will bring together professionals and experts in the agricultural and biological engineering fields. Bioenergy Technologies Office (BETO) Terrestrial Feedstocks Technology Manager Sam Tagore will be in attendance. Mr. Tagore will moderate a technical session titled “Ash Reduction Strategies for Improving Biomass Feedstock Quality.” The session will include presentations by researchers from Idaho National Laboratory and Oak Ridge National Laboratory supporting BETO, as well as from university and industry.

  15. Fuel Cell Technologies Overview

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Fuel Cell Seminar Orlando, FL Dr. Sunita Satyapal U.S. Department of Energy Fuel Cell Technologies Program Program Manager 11/1/2011 2 | Fuel Cell Technologies Program Source: US DOE 3/19/2013 eere.energy.gov DOE Program Overview Budget Progress Next Steps Agenda 3 | Fuel Cell Technologies Program Source: US DOE 3/19/2013 eere.energy.gov DOE Program Structure The Program is an integrated effort, structured to address all the key challenges and obstacles facing widespread commercialization. The

  16. OHVT technology roadmap [2000

    SciTech Connect (OSTI)

    Bradley, R.A.

    2000-02-01

    The OHVT Technology Roadmap for 2000 presents the multiyear program plan of the U.S. DOE's Office of Heavy Vehicle Technologies (OHVT). It is an update of the 1997 plan, reflecting changes in regulations and ongoing discussions with DOE's heavy vehicle customers. The technical plan covers three classes of trucks: (1) class 7-8 (large, on-highway trucks); (2) class 3-6 (medium duty trucks); (3) class 1-2 (pickups, vans, and sport utility vehicles) as well as enabling and supporting technologies. The Roadmap documents program goals, schedules, and milestones.

  17. DNA sequence determinants controlling affinity, stability and shape of DNA complexes bound by the nucleoid protein Fis

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Hancock, Stephen P.; Stella, Stefano; Cascio, Duilio; Johnson, Reid C.; Leng, Fenfei

    2016-03-09

    The abundant Fis nucleoid protein selectively binds poorly related DNA sequences with high affinities to regulate diverse DNA reactions. Fis binds DNA primarily through DNA backbone contacts and selects target sites by reading conformational properties of DNA sequences, most prominently intrinsic minor groove widths. High-affinity binding requires Fis-stabilized DNA conformational changes that vary depending on DNA sequence. In order to better understand the molecular basis for high affinity site recognition, we analyzed the effects of DNA sequence within and flanking the core Fis binding site on binding affinity and DNA structure. X-ray crystal structures of Fis-DNA complexes containing variable sequencesmore » in the noncontacted center of the binding site or variations within the major groove interfaces show that the DNA can adapt to the Fis dimer surface asymmetrically. We show that the presence and position of pyrimidine-purine base steps within the major groove interfaces affect both local DNA bending and minor groove compression to modulate affinities and lifetimes of Fis-DNA complexes. Sequences flanking the core binding site also modulate complex affinities, lifetimes, and the degree of local and global Fis-induced DNA bending. In particular, a G immediately upstream of the 15 bp core sequence inhibits binding and bending, and A-tracts within the flanking base pairs increase both complex lifetimes and global DNA curvatures. Taken together, our observations support a revised DNA motif specifying high-affinity Fis binding and highlight the range of conformations that Fis-bound DNA can adopt. Lastly, the affinities and DNA conformations of individual Fis-DNA complexes are likely to be tailored to their context-specific biological functions.« less

  18. Intriguing DNA Editor Has a Structural Trigger

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Intriguing DNA Editor Has a Structural Trigger Intriguing DNA Editor Has a Structural Trigger Print Wednesday, 30 July 2014 00:00 A powerful new tool for genome editing and gene regulation has emerged in the form of a family of enzymes known as Cas9. Cas9 could become an even more valuable tool with the creation of the first detailed picture of its three-dimensional shape. An international collaboration used x-ray crystallography to produce high-resolution structures of two major types of Cas9

  19. OMB August 1 2012 final

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    FORGE & EGS Program Outlook Courtesy Ben Phillips Courtesy FORO Energy Courtesy Schlumberger Courtesy Enel Green Power Courtesy Geothermal Resources Council Courtesy Bill Goloski 2 Energy Efficiency & Renewable Energy eere.energy.gov EGS Demonstration Portfolio Core Area Results Performer Project Site Site Information Status Funding Ormat Technologies Inc. Desert Peak, NV Adjacent to existing hydrothermal development Successful stimulation completed - 1.7 MW additional capacity added $

  20. EGS Success Stories | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    EGS Success Stories EGS Success Stories May 26, 2016 The team at Brady Hot Springs includes scientists and engineers from: University of Wisconsin-Madison Department of Geoscience; Ormat Technologies, Inc.; Silixa Ltd.; University of Nevada-Reno; Temple University; Lawrence Livermore National Laboratory; and Lawrence Berkeley National Laboratory. | Photo by Dan Koetke Nevada Site Home to Geothermal Community Focused on Expediting Research and Development Over the last six years, Brady Hot

  1. Energy Department Honored for Inroads in Geothermal Energy | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Honored for Inroads in Geothermal Energy Energy Department Honored for Inroads in Geothermal Energy June 9, 2015 - 11:23am Addthis Energy Department Honored for Inroads in Geothermal Energy The Energy Department's geothermal office garnered five awards at the Geothermal Energy Association's (GEA) National Geothermal Summit on June 3, 2015, amidst a celebrated list of twelve sector trailblazers and industry standouts, including Ormat Technologies, Enel Green Power of North America, and

  2. Time-lapse Joint Inversion of Geophysical Data and its Applications to Geothermal Prospecting

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Time-lapse Joint Inversion of Geophysical Data and its Applications to Geothermal Prospecting - GEODE Principal Investigators: Andre Revil and Mike Batzle (Colorado School of Mines) Ezra Zemach (ORMAT) Project Officer: Mark Ziegenbein Total Project Funding: $635,000 This presentation does not contain any proprietary confidential, or otherwise restricted information. April 22, 2013 DE-EE0005513 Component Technologies R&D: 2 | US DOE Geothermal Office eere.energy.gov Relevance/Impact of

  3. Science & Technology - 2014

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    august Science & Technology - 2014 August First Multi-bunch ... for the first time the generation of two nearly-identical ... emission, fuel motion, and mix levels in the hot-spot at ...

  4. Vehicle Technologies Program Overview

    SciTech Connect (OSTI)

    none,

    2006-09-05

    Overview of the Vehicle Technologies Program including external assessment and market view; internal assessment, program history and progress; program justification and federal role; program vision, mission, approach, strategic goals, outputs, and outcomes; and performance goals.

  5. Biogas Production Technologies

    Broader source: Energy.gov [DOE]

    Presentation about UC Davis's biogas technologies and integration with fuel cells. Presented by Ruihong Zhang, UC Davis, at the NREL/DOE Biogas and Fuel Cells Workshop held June 11-13, 2012, in Golden, Colorado.

  6. Energy Technology Engineering Center

    Office of Energy Efficiency and Renewable Energy (EERE)

    The Energy Technology Engineering Center (ETEC) is located within Area IV of the Santa Susana Field Laboratory. The ETEC occupies 90-acres within the 290 acre site. The Santa Susana Field...

  7. Technologies Taking Us There

    SciTech Connect (OSTI)

    Cotrell, Jason; Veers, Paul

    2015-09-29

    Keynote presentation at the Iowa State Wind Energy Symposium. This presentation examines several cutting-edge technologies and research being performed by the National Renewable Energy Laboratory that is helping achieve the U.S. Department of Energy's Wind Vision.

  8. Desalination technology evaluation

    SciTech Connect (OSTI)

    Del Bene, J.V.; Loh, G.T.; Schleicher, R.W.; Sgammato, T.A.; Sinha, A.K. )

    1992-12-01

    The shortage of potable water has hindered economic development in South Florida and other areas of the United States. This project, cosponsored with Florida Power Light (FPL), examines the economics of colocation of a water desalination plant with an electric power plant to take advantage of shared facilities, personnel, and equipments well as existing intake and outfall structures. In combination, these factors should reduce the cost of desalinated water. The first step in determining the viability of colocation is identification of desalination technologies best suited for dual-purpose applications in retrofits at existing fossil plants. Based on energy efficiency and commercial maturity, reverse osmosis (RO) and low-temperature multieffect distillation (LT-MED) technologies appear to be the best candidates for such application. In fact, RO provides the best economics for the plants and conditions studied. Of the emerging technologies evaluated, sodium molecular pumping and solvent extraction technologies should be further investigated for their potential in significantly reducing desalination costs.

  9. NREL: Technology Transfer - Webmaster

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Webmaster To report any problems on or ask a question about the NREL Technology Transfer Web site, you may contact the Webmaster using the online form below. If you have a question...

  10. Membrane Technology Workshop

    Broader source: Energy.gov [DOE]

    At the Membrane Technology Workshop (held July 24, 2012, in Rosemont, IL), stakeholders from industry and academia explored the status of membrane research and development (R&D). Participants ...

  11. ENERGY EFFICIENCY TECHNOLOGY ROADMAP

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    n E n v e l o p e This document is one component of the Energy Efficiency Technology Roadmap (EE Roadmap), published by the Bonneville Power Administration (BPA) on behalf of...

  12. ENERGY EFFICIENCY TECHNOLOGY ROADMAP

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    3 : L i g h t i n g This document is one component of the Energy Efficiency Technology Roadmap (EE Roadmap), published by the Bonneville Power Administration (BPA) on behalf of...

  13. Director, Building Technologies Office

    Broader source: Energy.gov [DOE]

    This position is located in the Building Technologies Office (BTO) of the Office of Energy Efficiency and Renewable Energy (EERE). The mission of EERE is to create and sustain American leadership...

  14. Quadrennial Technology Review 2015

    Broader source: Energy.gov (indexed) [DOE]

    program supported improvements in this technology, such as the use of nano-clay for next-generation HVDC cables. A research emphasis is also needed on superconducting HVDC cables,...

  15. TEAM Technologies, Inc.

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Business Pulsed Power Support TEAM Technologies Inc. opened its doors in 1985 as a one-man operation in support of Sandia's Z Machine, a mainstay of the Lab's Pulsed Power...

  16. Science & Technology - 2015

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Science & Technology - 2015 March Developing Algorithms for Laser-based 3D Metal Printing ... In order to print a 3D part using the SLM process, the user must enter data into the ...

  17. Geothermal Technologies Newsletter Archives

    Broader source: Energy.gov [DOE]

    Here you'll find past issues of the U.S. Department of Energy's (DOE) Geothermal Technologies program newsletter, which features information about its geothermal research and development efforts....

  18. TECHNOLOGY PROGRAM PLAN

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    DECEMBER 2014 CARBON STORAGE U.S. DEPARTMENT OF ENERGY TECHNOLOGY PROGRAM PLAN PREfACE ii DISCLAIMER ... within the context of an integrated system of capture, transport, and storage ...

  19. Science & Technology - 2015

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and finally compressed to a short pulse and highest peak power in large compressor vessels. The new front-end technology is based on a short-pulse optical-parametric...

  20. Technology catalogue. Second edition

    SciTech Connect (OSTI)

    1995-04-01

    The Department of Energy`s (DOE`s) Office of Environmental Management (EM) is responsible for remediating DOE contaminated sites and managing the DOE waste inventory in a safe and efficient manner. EM`s Office of Technology Development (OTD) supports applied research and demonstration efforts to develop and transfer innovative, cost-effective technologies to its site clean-up and waste-management programs within EM. The purpose of the Technology Catalogue is to: (a) provide performance data on OTD-developed technologies to scientists and engineers responsible for preparing Remedial Investigation/Feasibility Studies (RI/FSs) and other compliance documents for the DOE`s clean-up and waste-management programs; and (b) identify partnering and commercialization opportunities with industry, other federal and state agencies, and the academic community.

  1. Drilling technology/GDO

    SciTech Connect (OSTI)

    Kelsey, J.R.

    1985-01-01

    The Geothermal Technology Division of the US Department of Energy is sponsoring two programs related to drilling technology. The first is aimed at development of technology that will lead to reduced costs of drilling, completion, and logging of geothermal wells. This program has the official title ''Hard Rock Penetration Mechanics.'' The second program is intended to share with private industry the cost of development of technology that will result in solutions to the near term geothermal well problems. This program is referred to as the ''Geothermal Drilling Organization''. The Hard Rock Penetration Mechanics Program was funded at $2.65M in FY85 and the GDO was funded at $1.0M in FY85. This paper details the past year's activities and accomplishments and projects the plans for FY86 for these two programs.

  2. Catalog of CHP Technologies

    Office of Energy Efficiency and Renewable Energy (EERE)

    This 2015 Catalog of CHP Technologies provides an overview of how combined heat and power systems work and the key concepts of efficiency and power-to-heat ratios. The report also includes...

  3. Science, Technology & Engineering

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Alan Bishop selected to lead LANL Science, Technology & Engineering directorate August 17, 2012 LOS ALAMOS, NEW MEXICO, August 17, 2012-Los Alamos National Laboratory Director Charles McMillan announced today that after a yearlong, nationwide search, Alan Bishop has been selected to be the Laboratory's next Principal Associate Director for Science, Technology, and Engineering (PADSTE). Bishop has been acting in that role - 2 - since Aug. 29, 2011.Over the course of a distinguished 30-year

  4. NREL: Geothermal Technologies - Capabilities

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Capabilities The NREL geothermal team leverages its capabilities in several different areas to enhance the visibility of geothermal technologies. These areas include low-temperature resources; enhanced geothermal systems; strategic planning, analysis, and modeling; and project assessment. Low-Temperature Geothermal Resources NREL works to develop and deploy innovative new technologies that will help the geothermal community achieve widespread adoption of under-utilized low-temperature resources

  5. NREL: Geothermal Technologies - Projects

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Projects The NREL geothermal team is involved in various projects to help accelerate the development and deployment of clean, renewable geothermal technologies, including low-temperature resources; enhanced geothermal systems; strategic planning, analysis, and modeling; and project assessment. Low-Temperature Geothermal Resources NREL supports the U.S. Department of Energy's (DOE) Geothermal Technologies Office (GTO) through various collaborations that evaluate the levelized cost of electricity

  6. Genome Science/Technologies

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Genome Genome Science/Technologies Los Alamos using cutting-edge sequencing, finishing, and analysis, impact valuable genomic data. Srinivas Iyer Bioscience Group Leader Email Get Expertise David Bruce Bioscience Deputy Group Leader Email Momchilo Vuyisich Scientist Email Rebecca McDonald Bioscience Communications Email State-of-the art technology and extensive genomics expertise Protein research Read caption + Los Alamos National Laboratory graduate student, Patricia Langan, changes the

  7. Science & Technology - 2016

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    technology / 2016 / july Science & Technology - 2016 July 3D Printing Could Revolutionize Laser Development Three-month Turnaround for a New NIF Diagnostic Talk about being responsive to the needs of your customers. The NIF & Photon Science team developed and deployed a complex new diagnostic for NIF's radiation-effects source development program in just three months, from concept to execution. The diagnostic, dubbed Sentinel because of its resemblance to the squid-like

  8. Technology Commercialization Program 1991

    SciTech Connect (OSTI)

    Not Available

    1991-11-01

    This reference compilation describes the Technology Commercialization Program of the Department of Energy, Defense Programs. The compilation consists of two sections. Section 1, Plans and Procedures, describes the plans and procedures of the Defense Programs Technology Commercialization Program. The second section, Legislation and Policy, identifies legislation and policy related to the Program. The procedures for implementing statutory and regulatory requirements are evolving with time. This document will be periodically updated to reflect changes and new material.

  9. Long Term Innovative Technologies

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    DOE's Hydrogen and Fuel Cell Technologies, Fuel Cell Presolicitation Workshop Bryan Pivovar With Input/Feedback from Rod Borup (LANL), Debbie Myers (ANL), DOE and others as noted in presentation Lakewood, CO March 16, 2010 Long Term Innovative Technologies National Renewable Energy Laboratory Innovation for Our Energy Future Innovative/Long Term and RELEVANT Mission of DOE Mission of EERE (Applied Program) Mission of HFCT To enable the widespread commercialization of hydrogen and fuel cells in

  10. Fuel Cell Technologies Overview

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    4/3/2012 eere.energy.gov Fuel Cell Technologies Overview Flow Cell Workshop Washington, DC Dr. Sunita Satyapal & Dr. Dimitrios Papageorgopoulos U.S. Department of Energy Fuel Cell Technologies Program 3/7/2011 Flow Cells for Energy Storage Workshop Purpose To understand the applied research and development needs and the grand challenges for the use of flow cells as energy-storage devices. Objectives 1. Understand the needs for applied research from stakeholders. 2. Gather input for future

  11. 1 | Bioenergy Technologies Office

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    PNNL Fuel Synthesis Catalyst - CRADA with W. R. GRACE May 21, 2013 Technology Area Review: Gasification Principal Investigators: Richard T. Hallen - PNNL David G. Ward - W, R. Grace WBS 3.3.2.7 Organizations: PNNL/W. R. Grace Presenter: Jonathan L. Male This presentation does not contain any proprietary, confidential, or otherwise restricted information 2 | Bioenergy Technologies Office eere.energy.gov Goal/Objective Statement The objective of this CRADA project was to team with W. R. Grace in

  12. Crosscutting Technology Research

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Crosscutting Technology Research The Crosscutting Technology Research program serves as a bridge between basic and applied research by fostering R&D in sensors and controls, modeling and simulation, and high performance materials. These activities target enhanced availability and cost reduction for advanced power systems. The Crosscutting program facilitates its R&D efforts through collaboration with other government agencies, large and small businesses, and universities. The

  13. Vehicle Technologies Office Overview

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Annual Merit Review and Peer Evaluation Meeting Christy Cooper Acting Director Vehicle Technologies Office June 6, 2016 2 Today: * What's new in VTO * Priorities and Initiatives * Hot Off the Press! 3 Analysis Jacob Ward Rachael Nealer Communications and Education Connie Bezanson Budget Cindy McMullen Office Administration Bernadette Jackson Operations Supervisor Christy Cooper Director Christy Cooper (Acting) Hybrid Electric Systems Program Manager David Howell Materials Technology Program

  14. Sequential addition of short DNA oligos in DNA-polymerase-based synthesis reactions

    DOE Patents [OSTI]

    Gardner, Shea N.; Mariella, Jr., Raymond P.; Christian, Allen T.; Young, Jennifer A.; Clague, David S.

    2011-01-18

    A method of fabricating a DNA molecule of user-defined sequence. The method comprises the steps of preselecting a multiplicity of DNA sequence segments that will comprise the DNA molecule of user-defined sequence, separating the DNA sequence segments temporally, and combining the multiplicity of DNA sequence segments with at least one polymerase enzyme wherein the multiplicity of DNA sequence segments join to produce the DNA molecule of user-defined sequence. Sequence segments may be of length n, where n is an even or odd integer. In one embodiment the length of desired hybridizing overlap is specified by the user and the sequences and the protocol for combining them are guided by computational (bioinformatics) predictions. In one embodiment sequence segments are combined from multiple reading frames to span the same region of a sequence, so that multiple desired hybridizations may occur with different overlap lengths. In one embodiment starting sequence fragments are of different lengths, n, n+1, n+2, etc.

  15. Technology's Impact on Production

    SciTech Connect (OSTI)

    Rachel Amann; Ellis Deweese; Deborah Shipman

    2009-06-30

    As part of a cooperative agreement with the United States Department of Energy (DOE) - entitled Technology's Impact on Production: Developing Environmental Solutions at the State and National Level - the Interstate Oil and Gas Compact Commission (IOGCC) has been tasked with assisting state governments in the effective, efficient, and environmentally sound regulation of the exploration and production of natural gas and crude oil, specifically in relation to orphaned and abandoned wells and wells nearing the end of productive life. Project goals include: (1) Developing (a) a model framework for prioritization and ranking of orphaned or abandoned well sites; (b) a model framework for disbursement of Energy Policy Act of 2005 funding; and (c) a research study regarding the current status of orphaned wells in the nation. (2) Researching the impact of new technologies on environmental protection from a regulatory perspective. Research will identify and document (a) state reactions to changing technology and knowledge; (b) how those reactions support state environmental conservation and public health; and (c) the impact of those reactions on oil and natural gas production. (3) Assessing emergent technology issues associated with wells nearing the end of productive life. Including: (a) location of orphaned and abandoned well sites; (b) well site remediation; (c) plugging materials; (d) plug placement; (e) the current regulatory environment; and (f) the identification of emergent technologies affecting end of life wells. New Energy Technologies - Regulating Change, is the result of research performed for Tasks 2 and 3.

  16. Information technology resources assessment

    SciTech Connect (OSTI)

    Stevens, D.F.

    1992-01-01

    This year`s Information Technology Resources Assessment (ITRA) is something of a departure from traditional practice. Past assessments have concentrated on developments in fundamental technology, particularly with respect to hardware. They form an impressive chronicle of decreasing cycle times, increasing densities, decreasing costs (or, equivalently, increasing capacity and capability per dollar spent), and new system architectures, with a leavening of operating systems and languages. Past assessments have aimed -- and succeeded -- at putting information technology squarely in the spotlight; by contrast, in the first part of this assessment, we would like to move it to the background, and encourage the reader to reflect less on the continuing technological miracles of miniaturization in space and time and more on the second- and third-order implications of some possible workplace applications of these miracles. This Information Technology Resources Assessment is intended to provide a sense of technological direction for planners in projecting the hardware, software, and human resources necessary to support the diverse IT requirements of the various components of the DOE community. It is also intended to provide a sense of our new understanding of the place of IT in our organizations.

  17. Information technology resources assessment

    SciTech Connect (OSTI)

    Stevens, D.F.

    1992-01-01

    This year's Information Technology Resources Assessment (ITRA) is something of a departure from traditional practice. Past assessments have concentrated on developments in fundamental technology, particularly with respect to hardware. They form an impressive chronicle of decreasing cycle times, increasing densities, decreasing costs (or, equivalently, increasing capacity and capability per dollar spent), and new system architectures, with a leavening of operating systems and languages. Past assessments have aimed -- and succeeded -- at putting information technology squarely in the spotlight; by contrast, in the first part of this assessment, we would like to move it to the background, and encourage the reader to reflect less on the continuing technological miracles of miniaturization in space and time and more on the second- and third-order implications of some possible workplace applications of these miracles. This Information Technology Resources Assessment is intended to provide a sense of technological direction for planners in projecting the hardware, software, and human resources necessary to support the diverse IT requirements of the various components of the DOE community. It is also intended to provide a sense of our new understanding of the place of IT in our organizations.

  18. Information technology resources assessment

    SciTech Connect (OSTI)

    Loken, S.C.

    1993-01-01

    The emphasis in Information Technology (IT) development has shifted from technology management to information management, and the tools of information management are increasingly at the disposal of end-users, people who deal with information. Moreover, the interactive capabilities of technologies such as hypertext, scientific visualization, virtual reality, video conferencing, and even database management systems have placed in the hands of users a significant amount of discretion over how these resources will be used. The emergence of high-performance networks, as well as network operating systems, improved interoperability, and platform independence of applications will eliminate technical barriers to the use of data, increase the power and range of resources that can be used cooperatively, and open up a wealth of possibilities for new applications. The very scope of these prospects for the immediate future is a problem for the IT planner or administrator. Technology procurement and implementation, integration of new technologies into the existing infrastructure, cost recovery and usage of networks and networked resources, training issues, and security concerns such as data protection and access to experiments are just some of the issues that need to be considered in the emerging IT environment. As managers we must use technology to improve competitiveness. When procuring new systems, we must take advantage of scalable resources. New resources such as distributed file systems can improve access to and efficiency of existing operating systems. In addition, we must assess opportunities to improve information worker productivity and information management through tedmologies such as distributed computational visualization and teleseminar applications.

  19. Office of Technology Transitions

    Broader source: Energy.gov [DOE]

    DOE's Technology Commercialization activities in 2009-13 have involved three broad areas of focus. The primary focus of technology commercialization has continued to be through new technologies developed at the National Laboratories and Facilities. As a second focus, to support and streamline commercialization of these DOE technologies, DOE has carried out a number of new initiatives and pilot projects. Finally, DOE's Department-wide commitment to using commercialization as one mechanism to support U.S. economic growth has led to new cross-cutting programs. U.S. Department of Energy researchers won 31 of the 100 awards in 2014, 36 awards in each of 2013, 2012 and 2011, and 46 in 2010, for a total of 185 over the period of 2009-13. A subset of these awards and other DOE developed technologies are described in Appendix E. These represent a spectrum of commercial areas including DOE mission areas of energy, efficiency, environment and security, as well as spin-off applications in the agricultural, aeronautical, medical, semiconductor and information technology industries, and broad applications in cyber security and sensing/control systems.

  20. Turbine imaging technology assessment

    SciTech Connect (OSTI)

    Moursund, R. A.; Carlson, T. J.

    2004-12-01

    The goal of this project was to identify and evaluate imaging technologies for observing juvenile fish within a Kaplan turbine, and specifically that would enable scientists to determine mechanisms of fish injury within an operating turbine unit. This report documents the opportunities and constraints for observing juvenile fish at specific locations during turbine passage. These observations were used to make modifications to dam structures and operations to improve conditions for fish passage while maintaining or improving hydropower production. The physical and hydraulic environment that fish experience as they pass through the hydroelectric plants were studied and the regions with the greatest potential for injury were defined. Biological response data were also studied to determine the probable types of injuries sustained in the turbine intake and what types of injuries are detectable with imaging technologies. The study grouped injury-causing mechanisms into two categories: fluid (pressure/cavitation, shear, turbulence) and mechanical (strike/collision, grinding/pinching, scraping). The physical constraints of the environment, together with the likely types of injuries to fish, provided the parameters needed for a rigorous imaging technology evaluation. Types of technology evaluated included both tracking and imaging systems using acoustic technologies (such as sonar and acoustic tags) and optic technologies (such as pulsed-laser videography, which is high-speed videography using a laser as the flash). Criteria for determining image data quality such as frame rate, target detectability, and resolution were used to quantify the minimum requirements of an imaging sensor.

  1. Nuclear Reactors and Technology

    SciTech Connect (OSTI)

    Cason, D.L.; Hicks, S.C.

    1992-01-01

    This publication Nuclear Reactors and Technology (NRT) announces on a monthly basis the current worldwide information available from the open literature on nuclear reactors and technology, including all aspects of power reactors, components and accessories, fuel elements, control systems, and materials. This publication contains the abstracts of DOE reports, journal articles, conference papers, patents, theses, and monographs added to the Energy Science and Technology Database during the past month. Also included are US information obtained through acquisition programs or interagency agreements and international information obtained through the International Energy Agency`s Energy Technology Data Exchange or government-to-government agreements. The digests in NRT and other citations to information on nuclear reactors back to 1948 are available for online searching and retrieval on the Energy Science and Technology Database and Nuclear Science Abstracts (NSA) database. Current information, added daily to the Energy Science and Technology Database, is available to DOE and its contractors through the DOE Integrated Technical Information System. Customized profiles can be developed to provide current information to meet each user`s needs.

  2. Vehicle Technologies Office Merit Review 2015: Vehicle Technologies Office

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Overview | Department of Energy Vehicle Technologies Office Overview Vehicle Technologies Office Merit Review 2015: Vehicle Technologies Office Overview Presentation given by U.S. Department of Energy at 2015 DOE Hydrogen and Fuel Cells Program and Vehicle Technologies Office Annual Merit Review and Peer Evaluation meeting about Vehicle Technologies Office overview. 02_howell_plenary_2015_amr.pdf (3.45 MB) More Documents & Publications Vehicle Technologies Office Merit Review 2016:

  3. Vehicle Technologies Office: 2014 Electric Drive Technologies Annual

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Progress Report | Department of Energy Electric Drive Technologies Annual Progress Report Vehicle Technologies Office: 2014 Electric Drive Technologies Annual Progress Report The Electric Drive Technologies research and development (R&D) subprogram within the DOE Vehicle Technologies Office (VTO) provides support and guidance for many cutting-edge automotive technologies under development. Research is focused on developing power electronics (PE), electric motor, and traction drive system

  4. Life-cycle Analysis of Geothermal Technologies; 2010 Geothermal Technology

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Program Peer Review Report | Department of Energy cycle Analysis of Geothermal Technologies; 2010 Geothermal Technology Program Peer Review Report Life-cycle Analysis of Geothermal Technologies; 2010 Geothermal Technology Program Peer Review Report DOE 2010 Geothermal Technologies Program Peer Review adse_005_wang.pdf (192.84 KB) More Documents & Publications Detecting Fractures Using Technology at High Temperatures and Depths - Geothermal Ultrasonic Fracture Imager (GUFI); 2010

  5. Technology Maturation Plan (TMP) Wet Air Oxidation (WAO) Technology for

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Tank 48H Treatment Project (TTP) | Department of Energy Wet Air Oxidation (WAO) Technology for Tank 48H Treatment Project (TTP) Technology Maturation Plan (TMP) Wet Air Oxidation (WAO) Technology for Tank 48H Treatment Project (TTP) This assessment determines the technology maturity level of the candidate Tank 48H treatment technologies that are being considered for implementation at DOE's SRS - specifically Wet Air Oxidation. Technology Maturation Plan (TMP) Wet Air Oxidation (WAO)

  6. Vehicle Technologies Office Merit Review 2015: Consumer Vehicle Technology

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Data | Department of Energy Consumer Vehicle Technology Data Vehicle Technologies Office Merit Review 2015: Consumer Vehicle Technology Data Presentation given by National Renewable Energy Laboratory at 2015 DOE Hydrogen and Fuel Cells Program and Vehicle Technologies Office Annual Merit Review and Peer Evaluation Meeting about consumer vehicle technology data. van003_singer_2015_o.pdf (546.73 KB) More Documents & Publications Vehicle Technologies Office Merit Review 2014: Consumer

  7. Flow cytometric measurement of total DNA and incorporated halodeoxyuridine

    DOE Patents [OSTI]

    Dolbeare, Frank A.; Gray, Joe W.

    1986-01-01

    A method for the simultaneous flow cytometric measurement of the total DNA content and the level of DNA synthesis in normal and malignant cells is disclosed. The sensitivity of the method allows a study of cell cycle traverse rates for large scale cell populations as well as single cell measurements. A DNA stain such as propidium iodide is used as the probe for the measurement of total DNA content and a monoclonal antibody reactive with a DNA precursor such as bromodeoxyuridine (BrdU) is used as a probe for the measurement of BrdU uptake by the cells as a measure of DNA synthesis.

  8. Science and Technology in the Physics and Advanced Technologies Directorate

    SciTech Connect (OSTI)

    Wootton, A J

    2004-11-16

    A compendium of LLNL Science and Technology Review articles involving scientist and engineers from the Physics and Advanced Technologies Directorate, from January 2002 to the present.

  9. MHK Technologies/CETO Wave Energy Technology | Open Energy Information

    Open Energy Info (EERE)

    Type Click here Point Absorber Technology Readiness Level Click here TRL 78: Open Water System Testing & Demonstration & Operation Technology Description The CETO system...

  10. Kilowatt Reactor Using Stirling TechnologY (KRUSTY) Demonstration...

    Office of Scientific and Technical Information (OSTI)

    (KRUSTY) Demonstration. CEDT Phase 1 Preliminary Design Documentation Citation Details In-Document Search Title: Kilowatt Reactor Using Stirling TechnologY (KRUSTY) Demonstration. ...

  11. MHK Technologies/Oregon State University Columbia Power Technologies...

    Open Energy Info (EERE)

    here Point Absorber Technology Readiness Level Click here TRL 1-3: Discovery Concept Definition Early Stage Development & Design & Engineering Technology Description When...

  12. Vehicle Technologies Office: 2014 Fuel and Lubricant Technologies...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Vehicle Technologies Office: 2014 Fuel and Lubricant Technologies R&D Annual Progress Report This report describes the progress made on the research and development projects funded ...

  13. Vehicle Technologies Office: 2013 Fuel and Lubricant Technologies...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Vehicle Technologies Office: 2013 Fuel and Lubricant Technologies R&D Annual Progress Report This report describes the progress made on the research and development projects funded ...

  14. Trusted Computing Technologies, Intel Trusted Execution Technology.

    SciTech Connect (OSTI)

    Guise, Max Joseph; Wendt, Jeremy Daniel

    2011-01-01

    We describe the current state-of-the-art in Trusted Computing Technologies - focusing mainly on Intel's Trusted Execution Technology (TXT). This document is based on existing documentation and tests of two existing TXT-based systems: Intel's Trusted Boot and Invisible Things Lab's Qubes OS. We describe what features are lacking in current implementations, describe what a mature system could provide, and present a list of developments to watch. Critical systems perform operation-critical computations on high importance data. In such systems, the inputs, computation steps, and outputs may be highly sensitive. Sensitive components must be protected from both unauthorized release, and unauthorized alteration: Unauthorized users should not access the sensitive input and sensitive output data, nor be able to alter them; the computation contains intermediate data with the same requirements, and executes algorithms that the unauthorized should not be able to know or alter. Due to various system requirements, such critical systems are frequently built from commercial hardware, employ commercial software, and require network access. These hardware, software, and network system components increase the risk that sensitive input data, computation, and output data may be compromised.

  15. Wind Technologies & Evolving Opportunities (Presentation)

    SciTech Connect (OSTI)

    Robichaud, R.

    2014-07-01

    This presentation covers opportunities for wind technology; wind energy market trends; an overview of the National Wind Technology Center near Boulder, Colorado; wind energy price and cost trends; wind turbine technology improvements; and wind resource characterization improvements.

  16. Technology-to-Market Initiative

    Broader source: Energy.gov [DOE]

    BTO’s Technology-to-Market (T2M) team drives high impact technologies from R&D to market readiness, preparing these technologies for real building demonstration, market deployment, and ultimately mass-market adoption.

  17. Industrial Process Heating - Technology Assessment

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Industrial Process Heating - Technology Assessment 1 2 Contents 3 4 1. Introduction to the Technology/System ............................................................................................... 2 5 1.1. Industrial Process Heating Overview ............................................................................................ 2 6 2. Technology Assessment and Potential ................................................................................................. 6 7 2.1. Status

  18. Suspending DNA origami between four gold nanodots

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Morales, Piero; Wang, Liqian; Krissanaprasit, Abhichart; Dalmastri, Claudia; Caruso, Mario N.; De Stefano, Mattia; Mosiello, Lucia; Rapone, Bruno; Rinaldi, Antonio; Vespucci, Stefano; et al

    2015-01-01

    Here, connecting DNA nanostructures to metallic nanostructures at specific positions is a relatively rarely addressed issue in nanotechnology.[1-5] It is of high importance for application of the origami structures as breadboards for molecular electronics and nanosensing arrays since the metallic nanostructures may potentially serve as electrodes.

  19. Chromosome-specific DNA Repeat Probes

    SciTech Connect (OSTI)

    Baumgartner, Adolf; Weier, Jingly Fung; Weier, Heinz-Ulrich G.

    2006-03-16

    In research as well as in clinical applications, fluorescence in situ hybridization (FISH) has gained increasing popularity as a highly sensitive technique to study cytogenetic changes. Today, hundreds of commercially available DNA probes serve the basic needs of the biomedical research community. Widespread applications, however, are often limited by the lack of appropriately labeled, specific nucleic acid probes. We describe two approaches for an expeditious preparation of chromosome-specific DNAs and the subsequent probe labeling with reporter molecules of choice. The described techniques allow the preparation of highly specific DNA repeat probes suitable for enumeration of chromosomes in interphase cell nuclei or tissue sections. In addition, there is no need for chromosome enrichment by flow cytometry and sorting or molecular cloning. Our PCR-based method uses either bacterial artificial chromosomes or human genomic DNA as templates with {alpha}-satellite-specific primers. Here we demonstrate the production of fluorochrome-labeled DNA repeat probes specific for human chromosomes 17 and 18 in just a few days without the need for highly specialized equipment and without the limitation to only a few fluorochrome labels.

  20. Ceramic Technology Project

    SciTech Connect (OSTI)

    Not Available

    1992-03-01

    The Ceramic Technology Project was developed by the USDOE Office of Transportation Systems (OTS) in Conservation and Renewable Energy. This project, part of the OTS's Materials Development Program, was developed to meet the ceramic technology requirements of the OTS's automotive technology programs. Significant accomplishments in fabricating ceramic components for the USDOE and NASA advanced heat engine programs have provided evidence that the operation of ceramic parts in high-temperature engine environments is feasible. These programs have also demonstrated that additional research is needed in materials and processing development, design methodology, and data base and life prediction before industry will have a sufficient technology base from which to produce reliable cost-effective ceramic engine components commercially. A five-year project plan was developed with extensive input from private industry. In July 1990 the original plan was updated through the estimated completion of development in 1993. The objective is to develop the industrial technology base required for reliable ceramics for application in advanced automotive heat engines. The project approach includes determining the mechanisms controlling reliability, improving processes for fabricating existing ceramics, developing new materials with increased reliability, and testing these materials in simulated engine environments to confirm reliability. Although this is a generic materials project, the focus is on the structural ceramics for advanced gas turbine and diesel engines, ceramic bearings and attachments, and ceramic coatings for thermal barrier and wear applications in these engines. To facilitate the rapid transfer of this technology to US industry, the major portion of the work is being done in the ceramic industry, with technological support from government laboratories, other industrial laboratories, and universities.

  1. 1366 TECHNOLOGIES | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    1366 TECHNOLOGIES 1366 TECHNOLOGIES 1366 TECHNOLOGIES 1366 TECHNOLOGIES PROJECT SUMMARY In September 2011, the Department of Energy issued a $150 million loan guarantee to finance 1366 Technologies to build innovative, new manufacturing facilities that produce silicon wafers for solar cells at less than half of today's cost. Under the terms of the loan, 1366 Technologies must achieve certain production milestones at its Bedford, Massachusetts demonstration manufacturing facility before receiving

  2. Technology Validation | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    Technology for Tank 48H Treatment Project (TTP) | Department of Energy Fluidized Bed Steam Reformer System. Technology Maturation Plan (TMP) Fluidized Bed Steam Reforming (FBSR) Technology for Tank 48H Treatment Project (TTP) (308.93 KB) More Documents & Publications Technology Maturation Plan (TMP) Wet Air Oxidation (WAO) Technology for Tank Tank 48H Treatment Project (TTP) | Department of Energy

    Wet Air Oxidation. Technology Maturation Plan (TMP) Wet Air Oxidation (WAO)

  3. Structures of Clamp-Loader Complexes Are Key to DNA Replication

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    are also crucial components of various other cellular pathways, such as DNA repair, cell cycle control, and chromatin structure. Sliding DNA clamps are loaded onto DNA by...

  4. Video Resources on Geothermal Technologies

    Broader source: Energy.gov [DOE]

    Geothermal video offerings at the Department of Energy include simple interactive illustrations of geothermal power technologies and interviews on initiatives in the Geothermal Technologies Office.

  5. Budasolar Technologies | Open Energy Information

    Open Energy Info (EERE)

    Jump to: navigation, search Name: Budasolar Technologies Place: Budapest, Hungary Zip: H - 1121 Sector: Solar Product: BudaSolar is a developer of thin film technologies and...

  6. Conservation Technologies | Open Energy Information

    Open Energy Info (EERE)

    by expanding it. Conservation Technologies is a company located in Duluth, Minnesota. Conservation Technologies specializes in energy efficiency in building construction, and...

  7. Mears Technology | Open Energy Information

    Open Energy Info (EERE)

    Waltham, Massachusetts Zip: 2451 Sector: Solar Product: Waltham-based developer of manufacturing technology for semiconductor chip producers. The firm's MEARS Silicon Technology...

  8. Ardica Technologies | Open Energy Information

    Open Energy Info (EERE)

    Ardica Technologies Place: San Francisco, California Zip: 94107 Product: Micro fuel cell company focused on customer-centric applications of fuel cell technologies. References:...

  9. Vehicle Technologies Office: Information Resources

    Broader source: Energy.gov [DOE]

    From here you can access additional information on advanced transportation technologies; view programmatic publications and technical information; learn the basics of hybrid vehicle technology;...

  10. Apex Technology | Open Energy Information

    Open Energy Info (EERE)

    Technology Jump to: navigation, search Name: Apex Technology Address: 2703 Merrywood Drive Place: Edison, NJ Zip: 08817 Website: www.apextgi.com Coordinates: 40.5288539,...

  11. Danen Technology | Open Energy Information

    Open Energy Info (EERE)

    Danen Technology Jump to: navigation, search Name: Danen Technology Place: Guanyin, Taiwan Product: PV ingot and wafer maker based in northern Taiwan. Coordinates: 25.032009,...

  12. Clearpower Technology | Open Energy Information

    Open Energy Info (EERE)

    Clearpower Technology Jump to: navigation, search Name: Clearpower Technology Place: Belfast, Northern Ireland, United Kingdom Zip: BT3 9DT Sector: Wind energy Product: Clearpower...

  13. Unimicron Technology | Open Energy Information

    Open Energy Info (EERE)

    Unimicron Technology Jump to: navigation, search Name: Unimicron Technology Place: Taoyuan, Taiwan Sector: Solar Product: Unimicron established solar venture jointly with United...

  14. Greenbox Technology | Open Energy Information

    Open Energy Info (EERE)

    Greenbox Technology Jump to: navigation, search Name: Greenbox Technology Place: San Bruno, California Zip: 94066 Sector: Carbon Product: California-based, interactive energy...

  15. Shenmao Technology | Open Energy Information

    Open Energy Info (EERE)

    Shenmao Technology Jump to: navigation, search Name: Shenmao Technology Place: Taoyuan, Taiwan Zip: 328 Product: Maker of solder paste and PV ribbons. Coordinates: 25.001909,...

  16. Technology Partners | Open Energy Information

    Open Energy Info (EERE)

    Partners Jump to: navigation, search Logo: Technology Partners Name: Technology Partners Address: 550 University Avenue Place: Palo Alto, California Zip: 94301 Region: Bay Area...

  17. Evince Technology | Open Energy Information

    Open Energy Info (EERE)

    Evince Technology Jump to: navigation, search Name: Evince Technology Place: United Kingdom Sector: Efficiency, Wind energy Product: String representation "Evince has pion ... ing...

  18. Greenward Technologies | Open Energy Information

    Open Energy Info (EERE)

    Technologies Jump to: navigation, search Name: Greenward Technologies Address: PO Box 203814 Place: Austin, Texas Zip: 78720 Region: Texas Area Sector: Wind energy Product:...

  19. Fuel Cell Technologies Office: Publications

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Fuel Cell Technologies Office EERE Fuel Cell Technologies Office Share this resource Publications Advanced Search Browse by Topic Mail Requests Help Feature featured product...

  20. Statpower Technologies | Open Energy Information

    Open Energy Info (EERE)

    Jump to: navigation, search Name: Statpower Technologies Place: British Columbia, Canada Zip: V5A 4B5 Product: Statpower Technologies develops and markets mobile and backup...

  1. MAK Technologies | Open Energy Information

    Open Energy Info (EERE)

    MAK Technologies Jump to: navigation, search Name: MAK Technologies Place: Lebanon, New Jersey Zip: 8833 Sector: Solar Product: Designs and installs solar electric and solar...

  2. Technology Application R&D

    Broader source: Energy.gov [DOE]

    Technology application R&D projects monitor SSL technology advances and provide laboratory and field evaluations of emerging products. Impartial, trusted analysis from DOE identifies and...

  3. EERE Fuel Cell Technologies Program

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AudienceEvent Date EERE Fuel Cell Technologies Program Sunita Satyapal Acting Program Manager U.S. Department of Energy Fuel Cell Technologies Program Fuel Cell Project Kickoff ...

  4. Energy Technologies | Department of Energy

    Office of Environmental Management (EM)

    Technologies Image of industrial pipes. District energy technologies-such as combined heat and power and microgrids-can help state, local, and tribal governments effectively...

  5. Wakonda Technologies | Open Energy Information

    Open Energy Info (EERE)

    Logo: Wakonda Technologies Name: Wakonda Technologies Address: 2A Gill Street Place: Woburn, Massachusetts Zip: 01801 Region: Greater Boston Area Sector: Solar Product: High...

  6. Geothermal Technologies | Open Energy Information

    Open Energy Info (EERE)

    Geothermal Technologies Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Print PDF Geothermal Technologies Geothermal energy can be utilized for electricity or heating...

  7. DOE Fuel Cell Technology Office

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Fuel Cell Technology Office - Sandia Energy Energy Search Icon Sandia Home Locations ... SunShot Grand Challenge: Regional Test Centers DOE Fuel Cell Technology Office Home...

  8. Project Information by Key Technology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... Technologies FWP-FEAA116 Corrosion Issues in Advanced Coal Fired Boilers ORNL - Oak Ridge National Laboratory Plant Optimization Technologies FWP-FEAA117 Materials ...

  9. Technology Integration | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    Program Annual Merit Review and Peer Evaluation Vehicle Technologies Plenary vtpn02smithti2011o.pdf (814.37 KB) More Documents & Publications Technology Integration Overview ...

  10. Composites Technology for Hydrogen Pipelines

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Composites Technology for Hydrogen Pipelines Barton Smith, Barbara Frame, Larry Anovitz ... for pipeline joining technologies Barton Smith 865-574-2196, smithdb@ornl.gov * Implement ...

  11. IPower Technologies | Open Energy Information

    Open Energy Info (EERE)

    Place: Andersen, Indiana Zip: 46013 Product: iPower Technologies provides advanced technologies and systems integration capabilities for the distributed generation market....

  12. DNA damage checkpoint recovery and cancer development

    SciTech Connect (OSTI)

    Wang, Haiyong; Zhang, Xiaoshan; Teng, Lisong; Legerski, Randy J.

    2015-06-10

    Cell cycle checkpoints were initially presumed to function as a regulator of cell cycle machinery in response to different genotoxic stresses, and later found to play an important role in the process of tumorigenesis by acting as a guard against DNA over-replication. As a counterpart of checkpoint activation, the checkpoint recovery machinery is working in opposition, aiming to reverse the checkpoint activation and resume the normal cell cycle. The DNA damage response (DDR) and oncogene induced senescence (OIS) are frequently found in precancerous lesions, and believed to constitute a barrier to tumorigenesis, however, the DDR and OIS have been observed to be diminished in advanced cancers of most tissue origins. These findings suggest that when progressing from pre-neoplastic lesions to cancer, DNA damage checkpoint barriers are overridden. How the DDR checkpoint is bypassed in this process remains largely unknown. Activated cytokine and growth factor-signaling pathways were very recently shown to suppress the DDR and to promote uncontrolled cell proliferation in the context of oncovirus infection. In recent decades, data from cell line and tumor models showed that a group of checkpoint recovery proteins function in promoting tumor progression; data from patient samples also showed overexpression of checkpoint recovery proteins in human cancer tissues and a correlation with patients' poor prognosis. In this review, the known cell cycle checkpoint recovery proteins and their roles in DNA damage checkpoint recovery are reviewed, as well as their implications in cancer development. This review also provides insight into the mechanism by which the DDR suppresses oncogene-driven tumorigenesis and tumor progression. - Highlights: • DNA damage checkpoint works as a barrier to cancer initiation. • DDR machinary response to genotoxic and oncogenic stress in similar way. • Checkpoint recovery pathways provide active signaling in cell cycle control. • Checkpoint

  13. Stirling technology development status

    SciTech Connect (OSTI)

    Dochat, G.R. ); Dudenhoefer, J.E. )

    1993-01-15

    Free-piston Stirling power converters have the potential to meet the many future space power requirements for a wide variety of applications with less mass, better efficiency, and less total area (collector and radiator) than other power converter options. These benefits result in significant dollar savings over the projected mission lifetime. The National Aeronautics and Space Administration (NASA)---Lewis Research Center (LeRC), which has the responsibility to evaluate and develop power technologies that can satisfy anticipated future space mission power requirements, has been developing free-piston Stirling power converters and is bringing the Stirling technology to readiness. As the principal contractor to NASA-LeRC, Mechanical Technology Incorporated (MTI) is under contract to develop the necessary space Stirling technology but also demonstrate the readiness of the technology in two generations of full-scale power converters. The first generation Stirling power converter, the component test power converter (CTPC), initiated cold end testing at the end of 1991, with hot testing scheduled during 1992. This paper reviews test progress of the CTPC including the initial hot engine test results. Modifications incorporated into the CTPC from the earlier space power demonstrator engine are reviewed as well.

  14. Mobil lube dewaxing technologies

    SciTech Connect (OSTI)

    Baker, C.L.; McGuiness, M.P.

    1995-09-01

    Currently, the lube refining industry is in a period of transition, with both hydroprocessing and catalytic dewaxing gathering momentum as replacements for solvent extraction and solvent dewaxing. In addition, lube product quality requirements have been increasing, both in the US and abroad. Mobil has developed a broad array of dewaxing catalytic technologies which can serve refiners throughout the stages of this transition. In the future, lube feedstocks which vary in source and wax content will become increasingly important, requiring an optimized system for highest performance. The Mobil Lube Dewaxing (MLDW) process is the work-horse of the catalytic dewaxing technologies, being a robust, low cost technology suitable for both solvent extracted and hydrocracked feeds. The Mobil Selective Dewaxing (MSDW) process has been recently introduced in response to the growth of hydroprocessing. MSDW requires either severely hydrotreated or hydrocracked feeds and provides improved lube yields and VI. For refiners with hydrocrackers and solvent dewaxing units, Mobil Wax Isomerization (MWI) technology can make higher VI base stocks to meet the growing demand for very high quality lube products. A review of these three technologies is presented in this paper.

  15. Lectin cDNA and transgenic plants derived therefrom

    DOE Patents [OSTI]

    Raikhel, Natasha V.

    2000-10-03

    Transgenic plants containing cDNA encoding Gramineae lectin are described. The plants preferably contain cDNA coding for barley lectin and store the lectin in the leaves. The transgenic plants, particularly the leaves exhibit insecticidal and fungicidal properties.

  16. DNA release from lipoplexes by anionic lipids: correlation with...

    Office of Scientific and Technical Information (OSTI)

    The separation of DNA from lipid measured this way was considerably slower and less ... This result was confirmed by centrifugal separation of released DNA and lipid. X-ray ...

  17. Flow cytometric measurement of total DNA and incorporated halodeoxyuridine

    DOE Patents [OSTI]

    Dolbeare, F.A.; Gray, J.W.

    1983-10-18

    A method for the simultaneous flow cylometric measurement of total cellular DNA content and of the uptake of DNA precursors as a measure of DNA synthesis during various phases of the cell cycle in normal and malignant cells in vitro and in vivo is described. The method comprises reacting cells with labelled halodeoxyuridine (HdU), partially denaturing cellular DNA, adding to the reaction medium monoclonal antibodies (mabs) reactive with HdU, reacting the bound mabs with a second labelled antibody, incubating the mixture with a DNA stain, and measuring simultaneously the intensity of the DNA stain as a measure of the total cellular DNA and the HdU incorporated as a measure of DNA synthesis. (ACR)

  18. Phosphoramide mustard exposure induces DNA adduct formation and the DNA damage repair response in rat ovarian granulosa cells

    SciTech Connect (OSTI)

    Ganesan, Shanthi Keating, Aileen F.

    2015-02-01

    Phosphoramide mustard (PM), the ovotoxic metabolite of the anti-cancer agent cyclophosphamide (CPA), destroys rapidly dividing cells by forming NOR-G-OH, NOR-G and G-NOR-G adducts with DNA, potentially leading to DNA damage. A previous study demonstrated that PM induces ovarian DNA damage in rat ovaries. To investigate whether PM induces DNA adduct formation, DNA damage and induction of the DNA repair response, rat spontaneously immortalized granulosa cells (SIGCs) were treated with vehicle control (1% DMSO) or PM (3 or 6 μM) for 24 or 48 h. Cell viability was reduced (P < 0.05) after 48 h of exposure to 3 or 6 μM PM. The NOR-G-OH DNA adduct was detected after 24 h of 6 μM PM exposure, while the more cytotoxic G-NOR-G DNA adduct was formed after 48 h by exposure to both PM concentrations. Phosphorylated H2AX (γH2AX), a marker of DNA double stranded break occurrence, was also increased by PM exposure, coincident with DNA adduct formation. Additionally, induction of genes (Atm, Parp1, Prkdc, Xrcc6, and Brca1) and proteins (ATM, γH2AX, PARP-1, PRKDC, XRCC6, and BRCA1) involved in DNA repair were observed in both a time- and dose-dependent manner. These data support that PM induces DNA adduct formation in ovarian granulosa cells, induces DNA damage and elicits the ovarian DNA repair response. - Highlights: • PM forms ovarian DNA adducts. • DNA damage marker γH2AX increased by PM exposure. • PM induces ovarian DNA double strand break repair.

  19. Deepwater seismic acquisition technology

    SciTech Connect (OSTI)

    Caldwell, J.

    1996-09-01

    Although truly new technology is not required for successful acquisition of seismic data in deep Gulf of Mexico waters, it is helpful to review some basic aspects of these seismic surveys. Additionally, such surveys are likely to see early use of some emerging new technology which can improve data quality. Because such items as depth imaging, borehole seismic, 4-D and marine 3-component recording were mentioned in the May 1996 issue of World Oil, they are not discussed again here. However, these technologies will also play some role in the deepwater seismic activities. What is covered in this paper are some new considerations for: (1) longer data records needed in deeper water, (2) some pros and cons of very long steamer use, and (3) two new commercial systems for quantifying data quality.

  20. Technology transfer 1995

    SciTech Connect (OSTI)

    Not Available

    1995-01-01

    Technology Transfer 1995 is intended to inform the US industrial and academic sectors about the many opportunities they have to form partnerships with the US Department of Energy (DOE) for the mutual advantage of the individual institutions, DOE, and the nation as a whole. It also describes some of the growing number of remarkable achievements resulting from such partnerships. These partnership success stories offer ample evidence that Americans are learning how to work together to secure major benefits for the nation--by combining the technological, scientific, and human resources resident in national laboratories with those in industry and academia. The benefits include more and better jobs for Americans, improved productivity and global competitiveness for technology-based industries, and a more efficient government laboratory system.