Sample records for dna lightning dock

  1. Lightning Dock Geothermal Space Heating Project: Lightning Dock...

    Open Energy Info (EERE)

    Dock KGRA, New Mexico Jump to: navigation, search OpenEI Reference LibraryAdd to library Report: Lightning Dock Geothermal Space Heating Project: Lightning Dock KGRA, New...

  2. Geothermal Literature Review At Lightning Dock Geothermal Area...

    Open Energy Info (EERE)

    Home Exploration Activity: Geothermal Literature Review At Lightning Dock Geothermal Area (Lienau, 1990) Exploration Activity Details Location Lightning Dock Geothermal Area...

  3. Geothermal Literature Review At Lightning Dock Geothermal Area...

    Open Energy Info (EERE)

    Lightning Dock Geothermal Area (Smith, 1978) Exploration Activity Details Location Lightning Dock Geothermal Area Exploration Technique Geothermal Literature Review Activity Date...

  4. Thermal Gradient Holes At Lightning Dock Geothermal Area (Cunniff...

    Open Energy Info (EERE)

    Thermal Gradient Holes At Lightning Dock Geothermal Area (Cunniff & Bowers, 2005) Exploration Activity Details Location Lightning Dock Geothermal Area Exploration Technique Thermal...

  5. Geothermal Literature Review At Lightning Dock Geothermal Area...

    Open Energy Info (EERE)

    Notes A look at EGS potential at Lightning Dock prepared by Lightning Dock Inc. and Ormat International Inc. Electrical market was considered as well as the thermal potential of...

  6. Lightning Dock KGRA, New Mexico's Largest Geothermal Greenhouse...

    Open Energy Info (EERE)

    (0) Retrieved from "http:en.openei.orgwindex.php?titleLightningDockKGRA,NewMexico%27sLargestGeothermalGreenhouse,LargestAquacultureFacility,andFirstBinaryE...

  7. Geology and geothermal waters of Lightning Dock region, Animas...

    Open Energy Info (EERE)

    Jump to: navigation, search OpenEI Reference LibraryAdd to library Journal Article: Geology and geothermal waters of Lightning Dock region, Animas Valley and Pyramid Mountains,...

  8. Geothermal Literature Review At Lightning Dock Geothermal Area...

    Open Energy Info (EERE)

    Rafferty, 1997) Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Geothermal Literature Review At Lightning Dock Geothermal Area (Rafferty, 1997)...

  9. Geothermal Literature Review At Lightning Dock Geothermal Area...

    Open Energy Info (EERE)

    Unknown Exploration Basis Results from the GRED program work at Lightning Dock lead to new ideas about the area Notes review of previous geologic and geophysical studies...

  10. Lightning Dock Geothermal Area | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere I Geothermal Pwer Plant Jump to:Landowners and WindLighting Control Design Jump to: navigation,Lightning Dock

  11. Lightning Dock II Geothermal Project | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere I Geothermal Pwer Plant Jump to:Landowners and WindLighting Control Design Jump to: navigation,Lightning

  12. Geophysics, Geology and Geothermal Leasing Status of the Lightning...

    Open Energy Info (EERE)

    Leasing Status of the Lightning Dock KGRA, Animas Valley, New Mexico Author C. Smith Published New Mexico Geological Society Guidebook, 1978 DOI Not Provided Check for DOI...

  13. Lightning Dock Geothermal Space Heating Project: Lightning Dock KGRA, New

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to: navigation, searchOf Kilauea Volcano,LakefrontLighthouse Solar Westchester JumpMexico | Open

  14. Lightning Dock Geothermal Area | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere I Geothermal Pwer Plant Jump to:Landowners and WindLighting Control Design Jump to: navigation,

  15. Lightning Dock Geothermal Facility | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to: navigation, searchOf Kilauea Volcano,LakefrontLighthouse Solar Westchester Jump to:Geothermal

  16. DNA . DNA

    E-Print Network [OSTI]

    1. DNA . , . , . . DNA DNA . , DNA . DNA . DNA . DNA DNA DNA . DNA [6, 7, 8]. DNA . DNA NACST/Sim DNA/DNA

  17. Direct-Current Resistivity Survey At Lightning Dock Area (Warpinski...

    Open Energy Info (EERE)

    not indicated DOE-funding Unknown Notes As a foundation for successful siting and drilling a deep test well, additional geophysical work has been completed including...

  18. Compound and Elemental Analysis At Lightning Dock Geothermal...

    Open Energy Info (EERE)

    DOE-funding Unknown Exploration Basis Part of the Geothermal Resource Evaluation and Definition (GRED) Program administered by DOE-AAO under Cooperative Agreement Number...

  19. Geothermal Literature Review At Lightning Dock Geothermal Area...

    Open Energy Info (EERE)

    literature and how it affects access to land and mineral rights for geothermal energy production References B. C. Farhar (2002) Geothermal Access to Federal and Tribal Lands: A...

  20. Compound and Elemental Analysis At Lightning Dock Geothermal...

    Open Energy Info (EERE)

    resource Notes The chemical analysis of water samples was used to try and identify a source reservoir temperature and reservoir rock type. References F. Dellechaie (1977) A...

  1. Thermal Gradient Holes At Lightning Dock Geothermal Area (Cunniff...

    Open Energy Info (EERE)

    R.A. Cunniff, R.L. Bowers (2003) Final Report: Enhanced Geothermal Systems Technology Phase II: Animas Valley, New Mexico Additional References Retrieved from "http:...

  2. Lightning Dock KGRA, New Mexico's Largest Geothermal Greenhouse, Largest

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere I Geothermal Pwer Plant Jump to:Landowners and WindLighting Control Design Jump to:

  3. Geothermal Literature Review At Lightning Dock Geothermal Area (Clemons, Et

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to: navigation, search OpenEI Reference LibraryAdd to libraryOpen Energy Information

  4. Geothermal Literature Review At Lightning Dock Geothermal Area...

    Open Energy Info (EERE)

    review; this paper covered geologic and geophysical data available at the time, as well as geochemical data to analyze the resource area. References Jonathan Callender...

  5. Geographic Information System At Lightning Dock Geothermal Area...

    Open Energy Info (EERE)

    for first pass geothermal site identification Notes NREL's Geothermal Prospector, has well data, land data, and other data to help make first pass identification of potential...

  6. Thermal Gradient Holes At Lightning Dock Geothermal Area (Arnold...

    Open Energy Info (EERE)

    DOE-funding Unknown Exploration Basis Known shallow hot spot in Animas Valley Notes Four thermal gradient holes were authorized to be drilled by AMEX, but no results were...

  7. Water Sampling At Lightning Dock Geothermal Area (Swanberg, 1976) | Open

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere IRaghuraji Agro IndustriesTown ofNationwideWTED Jump to: navigation,Area (Wood, 2002) Jump to:EnergyEnergy

  8. Well Deepening At Lightning Dock Geothermal Area (Witcher, 2006) | Open

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere IRaghuraji Agro IndustriesTown ofNationwideWTED Jump to:Ohio: EnergyWebGenWelcome Sample 1: RetrieveEnergy

  9. Cation Geothermometers At Lightning Dock Geothermal Area (Witcher, 2006) |

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand JumpConceptual Model, click here.Telluric Survey as explorationpage? ForChinaOpen Energy

  10. Evaluation of Geothermal Potential of Lightning Dock KGRA, New Mexico |

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand JumpConceptual Model,DOEHazelPennsylvania: Energy Resources(RECP) inEuricoOpen Energy Information

  11. Water Sampling At Lightning Dock Geothermal Area (Witcher, 2006) | Open

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to:Ezfeedflag JumpID-fTri GlobalJump to: navigation, searchOpen EnergyKauai Area (Thomas,Energy

  12. Production Wells At Lightning Dock Geothermal Area (Cyrq Energy, 2014) |

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to: navigation,Pillar Group BV Jump to: navigation,PowerInformationOpen Energy

  13. Isotope Geothermometry At Lightning Dock Geothermal Area (Witcher, 2006) |

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdfGetecGtelInterias Solar Energy JumpIrem GeothermalIselin,Isofoton SAOpen Energy

  14. Isotopic Analysis- Fluid At Lightning Dock Geothermal Area (Witcher, 2006)

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdfGetecGtelInterias Solar Energy JumpIremNot2007) ||Al., 1989) | OpenAl., 1993)

  15. Observation Wells At Lightning Dock Geothermal Area (Reeder, 1957) | Open

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to: navigation, searchOfRoseConcerns Jumpsource HistoryFractures belowOasisEnergy Information

  16. Lightning Dock Geothermal HI-01 | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to: navigation, searchOf Kilauea Volcano,LakefrontLighthouse Solar Westchester Jump

  17. Lightning Dock I Geothermal Project | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to: navigation, searchOf Kilauea Volcano,LakefrontLighthouse Solar Westchester JumpMexico | OpenI

  18. Ground Gravity Survey At Lightning Dock Geothermal Area (Swanberg, 1976) |

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdfGetec AG| Open Energy Information 2000) ExplorationAl., 1979) |Open Energy

  19. Geographic Information System At Lightning Dock Geothermal Area (Getman,

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdf Jump1946865°,Park,2005) | Open Energy Information(Blewitt, EtEt(Nash,

  20. Geophysics, Geology and Geothermal Leasing Status of the Lightning Dock

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdf Jump1946865°,Park,2005) | Open Energy(Blackwell,

  1. Geothermal Literature Review At Lightning Dock Geothermal Area (Callender,

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdf Jump1946865°,Park,2005)Energy Information ) Exploration2004) | Open1981)

  2. Geothermal Literature Review At Lightning Dock Geothermal Area (Dahal, Et

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdf Jump1946865°,Park,2005)Energy Information ) Exploration2004) |

  3. Geothermal Literature Review At Lightning Dock Geothermal Area (Elston, Et

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdf Jump1946865°,Park,2005)Energy Information ) Exploration2004) |Al., 1983)

  4. Geothermal Literature Review At Lightning Dock Geothermal Area (Farhar,

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdf Jump1946865°,Park,2005)Energy Information ) Exploration2004) |Al.,

  5. Geothermal Literature Review At Lightning Dock Geothermal Area (Fleischman,

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdf Jump1946865°,Park,2005)Energy Information ) Exploration2004) |Al.,2006) |

  6. Geothermal Literature Review At Lightning Dock Geothermal Area (Grant,

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdf Jump1946865°,Park,2005)Energy Information ) Exploration2004) |Al.,2006)

  7. Geothermal Literature Review At Lightning Dock Geothermal Area (Lienau,

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdf Jump1946865°,Park,2005)Energy Information ) Exploration2004)

  8. Geothermal Literature Review At Lightning Dock Geothermal Area (Parker &

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdf Jump1946865°,Park,2005)Energy Information ) Exploration2004)Icerman,

  9. Geothermal Literature Review At Lightning Dock Geothermal Area (Rafferty,

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdf Jump1946865°,Park,2005)Energy Information ) Exploration2004)Icerman,1997)

  10. Geothermal Literature Review At Lightning Dock Geothermal Area (Sammel,

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdf Jump1946865°,Park,2005)Energy Information )

  11. Geothermal Literature Review At Lightning Dock Geothermal Area (Schochet,

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdf Jump1946865°,Park,2005)Energy Information )Et Al., 2001) | Open Energy

  12. Geothermal Literature Review At Lightning Dock Geothermal Area (Smith,

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdf Jump1946865°,Park,2005)Energy Information )Et Al., 2001) | Open

  13. Geothermal Literature Review At Lightning Dock Geothermal Area (Spiegel,

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdf Jump1946865°,Park,2005)Energy Information )Et Al., 2001) | Open1957) |

  14. Geothermal Literature Review At Lightning Dock Geothermal Area (Stone, Et

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdf Jump1946865°,Park,2005)Energy Information )Et Al., 2001) | Open1957)

  15. Geothermal Literature Review At Lightning Dock Geothermal Area (Summers,

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdf Jump1946865°,Park,2005)Energy Information )Et Al., 2001) | Open1957)1976)

  16. Geothermal Literature Review At Lightning Dock Geothermal Area (Witcher,

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdf Jump1946865°,Park,2005)Energy Information )Et Al., 2001) |

  17. Geothermal Literature Review At Lightning Dock Geothermal Area (Witcher,

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdf Jump1946865°,Park,2005)Energy Information )Et Al., 2001) |2008) | Open

  18. Geothermal Literature Review At Lightning Dock Geothermal Area (Witcher, Et

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdf Jump1946865°,Park,2005)Energy Information )Et Al., 2001) |2008) |

  19. Compound and Elemental Analysis At Lightning Dock Geothermal Area

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand JumpConceptual Model, clickInformationNew York:GovernorCommons(Grigsby, Et| Open(Quane, EtEt

  20. Compound and Elemental Analysis At Lightning Dock Geothermal Area (Elston,

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand JumpConceptual Model, clickInformationNew York:GovernorCommons(Grigsby, Et| Open(Quane, EtEtEt

  1. Compound and Elemental Analysis At Lightning Dock Geothermal Area (Witcher,

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand JumpConceptual Model, clickInformationNew York:GovernorCommons(Grigsby, Et| Open(Quane,

  2. DockingShop: A Tool for Interactive Molecular Docking

    SciTech Connect (OSTI)

    Lu, Ting-Cheng; Max, Nelson L.; Ding, Jinhui; Bethel, E. Wes; Crivelli, Silvia N.

    2005-04-24T23:59:59.000Z

    Given two independently determined molecular structures, the molecular docking problem predicts the bound association, or best fit between them, while allowing for conformational changes of the individual molecules during construction of a molecular complex. Docking Shop is an integrated environment that permits interactive molecular docking by navigating a ligand or protein to an estimated binding site of a receptor with real-time graphical feedback of scoring factors as visual guides. Our program can be used to create initial configurations for a protein docking prediction process. Its output--the structure of aprotein-ligand or protein-protein complex--may serve as an input for aprotein docking algorithm, or an optimization process. This tool provides molecular graphics interfaces for structure modeling, interactive manipulation, navigation, optimization, and dynamic visualization to aid users steer the prediction process using their biological knowledge.

  3. DNA DNA DNA (d)DNA DNA DNA

    E-Print Network [OSTI]

    Hagiya, Masami

    DNA DNA DNA DNA DNA DNA DNA DNA [ 2008] (d)DNA DNA DNA DNA 2 3 DNA DNA DNA DNA DNA DNA DNA (a) (c) (b) (d) #12;DNA DNA DNA DNA DNA DNA DNA DNA (b) DNA [Tanaka et al.2008] DNA DNA DNA DNA DNA DNA DNA #12;iGEM MIT MIT

  4. Eddy Current Model of Ball Lightning

    E-Print Network [OSTI]

    J. D. Shelton

    2011-02-07T23:59:59.000Z

    Eddy Current Model of Ball Lightning Calculations show that high-energy ball lightning may consist of a ball of plasma containing a large circular electric current arising as an eddy current generated by lightning. Synthetic ball lightning might serve as a method of plasma confinement for purposes of nuclear fusion. In this paper, three articles concerning ball lightning and the related phenomenon of large ball lightning are combined to provide insight into this rarely glimpsed occurrence.

  5. DNA DNA . DNA

    E-Print Network [OSTI]

    1. DNA DNA , . . DNA ( ) DNA "exhaustive " . Boolean , 40 2 40 10 12 pico mole . DNA . 20 3-SAT DNA NP-complete [1]. [2, 3]. DNA

  6. ARM - Measurement - Lightning stroke

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625govInstrumentstdmadap Documentation TDMADAP : XDC documentationBarrow,icegovMeasurementsLightning stroke ARM Data

  7. A randomized global optimization method for protein-protein docking

    E-Print Network [OSTI]

    2003-04-10T23:59:59.000Z

    e.g., the well known problem of protein folding), when dealing with docking some important modifications are necessary. In particular, when rigid docking is...

  8. DNA -DNA--

    E-Print Network [OSTI]

    Glykos, Nikolaos

    #12;#12;#12;8.1 DNA - . DNA- - (DNA-binding domains) ( 100 ). - DNA- - -, DNA. - -- - DNA. 173 8 DNA : -- 8.1 8.2 8.3 Cro - 8.4 Cro 8.5 DNA- - 8.6 Cro DN 8

  9. The Houston Lightning Mapping Array: Network Installation and Preliminary Analysis

    E-Print Network [OSTI]

    Cullen, Matthew Ryan

    2013-06-25T23:59:59.000Z

    The Houston Lightning Mapping Array (LMA) is a lightning detection network providing total lightning mapping for the Houston metropolitan area and southeast Texas. The network is comprised of twelve Very High Frequency (VHF) time-of-arrival total...

  10. Changes in measured lightning return stroke peak current after the 1994 National Lightning Detection Network upgrade

    E-Print Network [OSTI]

    Wacker, Robert Scott

    1997-01-01T23:59:59.000Z

    Since a comprehensive upgrade of the US National Lightning Detection Network (NLDN) in 1994, the mean peak current of detected cloud-to-ground (CG) lightning flashes has decreased, the number of detected flashes has increased, and the percentage...

  11. Computational methods for molecular docking

    SciTech Connect (OSTI)

    Klebe, G. [BASF AG, Ludwigshafen (Germany); Lengauer, T.

    1995-12-31T23:59:59.000Z

    This tutorial was one of eight tutorials selected to be presented at the Third International Conference on Intelligent Systems for Molecular Biology which was held in the United Kingdom from July 16 to 19, 1995. Recently, it has been demonstrated that the knowledge of the three-dimensional structure of the protein can be used to derive new protein ligands with improved binding properties. This tutorial focuses on the following questions: What is its binding affinity toward a particular receptor? What are putative conformations of a ligand at the binding site? What are the similarities of different ligands in terms of their recognition capabilities? Where and in which orientation will a ligand bind to the active site? How is a new putative protein ligand selected? An overview is presented of the algorithms which are presently used to handle and predict protein-ligand interactions and to dock small molecule ligands into proteins.

  12. GWIDD: Genome-wide protein docking database

    E-Print Network [OSTI]

    Kundrotas, Petras J.; Zhu, Zhengwei; Vasker, Ilya A.

    2009-11-09T23:59:59.000Z

    Structural information on interacting proteins is important for understanding life processes at the molecular level. Genome-wide docking database is an integrated resource for structural studies of proteinprotein interactions on the genome scale...

  13. Flow Test At Lightning Dock Area (Cunniff & Bowers, 2005) | Open Energy

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directedAnnualPropertyd8c-a9ae-f8521cbb8489Information Hydro Inc IosilEnergyEnergy

  14. Fluid Inclusion Analysis At Lightning Dock Area (Norman & Moore, 2004) |

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directedAnnualPropertyd8c-a9ae-f8521cbb8489Information Hydro IncEnergyInformationOpen Energy

  15. Ground Gravity Survey At Lightning Dock Area (Warpinski, Et Al., 2002) |

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to: navigation, search OpenEI ReferenceJump to:InformationGroton Jump2004) | Open EnergyOpen

  16. Aeromagnetic Survey At Lightning Dock Area (Cunniff & Bowers, 2005) | Open

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directedAnnualProperty Edit withTianlinPapersWindeySanta2004) | OpenInformation Zablocki, 1978) Jump

  17. Aeromagnetic Survey At Lightning Dock Area (Warpinski, Et Al., 2002) | Open

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to:Ezfeedflag JumpID-fTriWildcat 1AMEE JumpAeroWind Inc. Place: Potsdam,Open EnergyEnergy

  18. Production Wells At Lightning Dock Geothermal Area (McCants, 1974) | Open

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to: navigation,Pillar Group BV Jump to: navigation,PowerInformationOpen EnergyEnergy

  19. Radiometrics At Lightning Dock Geothermal Area (Deal, Et Al., 1978) | Open

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to:Ezfeedflag JumpID-f < RAPID‎ | Roadmap Jump to:b <RGS Development

  20. Reflection Survey At Lightning Dock Area (Cunniff & Bowers, 2005) | Open

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to:Ezfeedflag JumpID-f < RAPID‎ | Roadmap Jump to:bJumpRed Bank,Reflection|Jemez Pueblo

  1. Ground Gravity Survey At Lightning Dock Area (Cunniff & Bowers, 2005) |

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdfGetec AG| Open Energy Information 2000) ExplorationAl., 1979) |

  2. Surface Gas Sampling At Lightning Dock Area (Norman & Moore, 2004) | Open

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere IRaghuraji Agro Industries PvtStratosolar Jump to:Holdings Co Ltd Place:Mclaren, 2010) | Open EnergyEnergy

  3. Surface Gas Sampling At Lightning Dock Area (Norman, Et Al., 2002) | Open

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere IRaghuraji Agro Industries PvtStratosolar Jump to:Holdings Co Ltd Place:Mclaren, 2010) | Open

  4. Numerical Modeling At Lightning Dock Geothermal Area (O'Brien, Et Al.,

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere I Geothermal Pwer PlantMunhall,Missouri: EnergyExcellence Seed LLCShores,ActivityNufcorEnergy2003) | Open1984)

  5. Observation Wells At Lightning Dock Area (Warpinski, Et Al., 2004) | Open

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere I Geothermal Pwer PlantMunhall,Missouri: EnergyExcellence SeedNunn,andOasys Water Jump to:Obetz,Energy

  6. Stepout-Deepening Wells At Lightning Dock Area (Warpinski, Et Al., 2004) |

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to:Ezfeedflag JumpID-f < RAPID‎SolarCityInformation Glass

  7. Surface Gas Sampling At Lightning Dock Area (Norman & Moore, 2004) | Open

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to:Ezfeedflag JumpID-f <Maintained By FaultSunpods Inc Jump to:Superior

  8. Surface Gas Sampling At Lightning Dock Area (Norman, Et Al., 2002) | Open

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to:Ezfeedflag JumpID-f <Maintained By FaultSunpods Inc Jump to:SuperiorEnergy Information

  9. Thermal Gradient Holes At Lightning Dock Area (Cunniff & Bowers, 2005) |

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to:Ezfeedflag JumpID-f <MaintainedInformationThe year openEnergy Information2004)

  10. Thermal Gradient Holes At Lightning Dock Geothermal Area (Arnold, Et Al.,

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to:Ezfeedflag JumpID-f <MaintainedInformationThe year openEnergy Information2004)1978)

  11. Thermal Gradient Holes At Lightning Dock Geothermal Area (Cunniff & Bowers,

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to:Ezfeedflag JumpID-f <MaintainedInformationThe year openEnergy

  12. Thermal Gradient Holes At Lightning Dock Geothermal Area (Cunniff, Et Al.,

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to:Ezfeedflag JumpID-f <MaintainedInformationThe year openEnergy2003) | Open Energy

  13. Direct-Current Resistivity Survey At Lightning Dock Area (Cunniff & Bowers,

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand JumpConceptual Model,DOE FacilityDimondale, Michigan: Energy Resources Jump(Thomas,(Thomas, 1986)

  14. Thermal Gradient Holes At Lightning Dock Area (Warpinski, Et Al., 2004) |

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere IRaghuraji Agro Industries PvtStratosolarTharaldson Ethanol LLCEnergy Information Szybinski, 2006)|

  15. Thermal Ion Dispersion At Lightning Dock Area (Cunniff & Bowers, 2005) |

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere IRaghuraji Agro Industries PvtStratosolarTharaldson Ethanol LLCEnergy InformationOpen Energy2004) |

  16. Analytical Modeling At Lightning Dock Geothermal Area (Brook, Et Al., 1978)

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to:Ezfeedflag JumpID-fTriWildcat Place:Alvan BlanchAmiteInExploration AtTransportation Sector in|

  17. Compound and Elemental Analysis At Lightning Dock Area (Norman & Moore,

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directedAnnualProperty EditCalifornia:PowerCER.png ElColumbia,2005) | Open(Thompson, 1985) | Open Energy2004)

  18. Gamma Log At Lightning Dock Geothermal Area (Witcher, 2006) | Open Energy

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdf Jump1946865°,Park, Texas: Energy Resources Jump81"Information

  19. Gas Flux Sampling At Lightning Dock Area (Cunniff & Bowers, 2005) | Open

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdf Jump1946865°,Park, Texas: EnergyGarvin County,| Open EnergyKawaihaeEnergy

  20. Geology and geothermal waters of Lightning Dock region, Animas Valley and

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdf Jump1946865°,Park,2005) | Open Energy(Blackwell, EtRaft riverPyramid

  1. Direct-Current Resistivity Survey At Lightning Dock Area (Warpinski, Et

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directedAnnualPropertyd8c-a9ae-f8521cbb8489 No revision| Open Energy Information At Lahaina-KaanapaliAl.,

  2. Crusader Automated Docking System Phase 3 report

    SciTech Connect (OSTI)

    Jatko, W.B.; Goddard, J.S.; Ferrell, R.K.; Gleason, S.S.; Hicks, J.S.; Varma, V.K.

    1996-03-01T23:59:59.000Z

    The US Army is developing the next generation of battlefield artillery vehicles, including an advanced, self-propelled howitzer and a companion resupply vehicle. The resupply vehicle is intended to rendezvous with the howitzer near the battlefront and to upload ammunition to the howitzer. The Army has recommended that the vehicles incorporate robotics to increase safety, by allowing the crew to remain inside their vehicles during resupply operations. Oak Ridge National Laboratory has developed an autonomous docking system for a 6-D.F. robotic, ammunition transfer arm. The docking system augments the operator`s abilities by determining the position and orientation (pose) of a docking port. The pose is the location of the x, y, and z reference axes in 3-D space; and the orientation is the rotations--roll, pitch, and yaw--about those axes. Bye precisely determining the pose of the docking port, the robot can be instructed to move to the docking position without operator intervention. The system uses a video camera and frame grabber to digitize images of the special docking port. Custom algorithms were developed to recognize the port in the camera image, to determine the pose from its image features, and to distribute the results to the robot control computer. The system is loosely coupled to the robot and can be easily adapted to different mechanical configurations. The system has successfully demonstrated autonomous docking on a 24-in. tabletop robot and a 12-ft ammunition resupply robot. The update rate, measurement accuracy, continuous operation, and accuracy with obstructed view have been determined experimentally.

  3. A comparative analysis of total lightning observations and cloud-to-ground lightning observations in the Southeastern United States region

    E-Print Network [OSTI]

    Hugo, Keith Michael

    1998-01-01T23:59:59.000Z

    Flashes of April 20, 1996, Orbit 13 After Performing Collocation Procedure. 19 21 5 OTD Versus NLDN Lightning Flashes. 24 6 OTD and NLDN Lightning Flashes of December 20, 1995, Orbit 2. 26 7 OTD and NLDN Lightning Flashes of July 23, 1996, Orbit 2... prototype for the Lightning Imaging Sensor launched aboard the Tropical Rainfall Measuring Mission (TRMM) [Goodman et aL, 1996], has enabled the detection of total lightning from space and allowed interesting comparisons of data coincident with ground...

  4. New mechanism for lightning initiation

    SciTech Connect (OSTI)

    Roussel-Dupre, R.; Buchwald, M.; Gurevich, A. [Lebedev Institute of Physical Sciences, Moscow (Russian Federation)] [and others

    1996-10-01T23:59:59.000Z

    This is the final report of a three-year, Laboratory-Directed Research and Development (LDRD) project at the Los Alamos National Laboratory (LANL). To distinguish radio-frequency (rf) signals generated by lightning from the electromagnetic pulse produced by a nuclear explosion, it is necessary to understand the fundamental nature of thunderstorm discharges. The recent debate surrounding the origin of transionospheric pulse pairs (TIPPs) detected by the BLACKBEARD experiment aboard the ALEXIS satellite illustrates this point. We have argued that TIPP events could originate from the upward propagating discharges recently identified by optical images taken from the ground, from airplanes, and from the space shuttle. In addition, the Gamma Ray Observatory (GRO) measurements of x-ray bursts originating from thunderstorms are almost certainly associated with these upward propagating discharges. When taken together, these three measurements point directly to the runaway electron mechanism as the source of the upward discharges. The primary goal of this research effort was to identify the specific role played by the runaway-air-breakdown mechanism in the general area of thunderstorm electricity and in so doing develop lightning models that predict the optical, rf, and x-ray emissions that are observable from space.

  5. Total lightning characteristics of ordinary convection

    E-Print Network [OSTI]

    Motley, Shane Michael

    2009-06-02T23:59:59.000Z

    processes involved in the electrical development of thunderstorms. Nine of the thunderstorm cases examined occurred within range of Vaisala Inc.'s Dallas-Fort Worth (DFW) Lightning Detection and Ranging (LDAR) network and the other thirteen cases occurred...

  6. VRDD: Applying virtual reality visualization to protein docking

    E-Print Network [OSTI]

    Weng, Zhiping

    the levels of atomic overlap and interac- tion energy. The stunning VR graphics immerse users in the scene. Keywords: virtual reality, computer graphics, CAVE, mo- lecular docking, binding free energy calculations molecular docking aided by automatic docking and side chain confor- mational search. Binding free energies

  7. Lightning protection system for a wind turbine

    DOE Patents [OSTI]

    Costin, Daniel P. (Chelsea, VT); Petter, Jeffrey K. (Williston, VT)

    2008-05-27T23:59:59.000Z

    In a wind turbine (104, 500, 704) having a plurality of blades (132, 404, 516, 744) and a blade rotor hub (120, 712), a lightning protection system (100, 504, 700) for conducting lightning strikes to any one of the blades and the region surrounding the blade hub along a path around the blade hub and critical components of the wind turbine, such as the generator (112, 716), gearbox (708) and main turbine bearings (176, 724).

  8. Lightning Arrestor Connectors Production Readiness

    SciTech Connect (OSTI)

    Marten, Steve; Linder, Kim; Emmons, Jim; Gomez, Antonio; Hasam, Dawud; Maurer, Michelle

    2008-10-20T23:59:59.000Z

    The Lightning Arrestor Connector (LAC), part M, presented opportunities to improve the processes used to fabricate LACs. The A## LACs were the first production LACs produced at the KCP, after the product was transferred from Pinnellas. The new LAC relied on the lessons learned from the A## LACs; however, additional improvements were needed to meet the required budget, yield, and schedule requirements. Improvement projects completed since 2001 include Hermetic Connector Sealing Improvement, Contact Assembly molding Improvement, development of a second vendor for LAC shells, general process improvement, tooling improvement, reduction of the LAC production cycle time, and documention of the LAC granule fabrication process. This report summarizes the accomplishments achieved in improving the LAC Production Readiness.

  9. Local Search Heuristic for Rigid Protein Docking

    E-Print Network [OSTI]

    Edelsbrunner, Herbert

    grants EIA-01-31905 and CCR-02-04118 and by the U.S.-Israel Binational Science Foundation. Departments the rigid docking problem and we refer to several survey articles in the general area [7,10, 13]. All

  10. Static electricity measurements for lightning warnings -an exploration

    E-Print Network [OSTI]

    Haak, Hein

    Static electricity measurements for lightning warnings - an exploration H. Bloemink De Bilt, 2013 | Internal report; IR 2013-01 #12;#12;Static electricity measurements for lightning warnings - an exploration Version 1.0 Date January 2013 Status Final #12;#12;Static electricity measurements for lightning warnings

  11. Lightning Strikes on Airborne Grounded Systems

    E-Print Network [OSTI]

    Malinga, Gilbert Aporu

    2014-10-13T23:59:59.000Z

    LIST OF FIGURES Page Fig. 1-1 Histogram of the average wind speed over a 10 year period at an elevation of a) sH ? 100 m and b) sH ? 600 m, above the mean water level at Montauk, New York. Wind data accessed from the National Buoy Data Center.... The destructive power of lightning discharges to both land-based and airborne systems that cannot adequately dissipate large impulses of energy is well documented (Miyake et al., 1990; Sorensen et al., 1998; Uman and Rakov, 2003). Lightning discharges can...

  12. Number of lightning discharges causing damage to lightning arrester cables for aerial transmission lines in power systems

    SciTech Connect (OSTI)

    Nikiforov, E. P. [Electric Power Scientific Research Institute (VNIIE), branch of OAO 'NTTs Elektroenergetika' (Russian Federation)

    2009-07-15T23:59:59.000Z

    Damage by lightning discharges to lightning arrester cables for 110-175 kV aerial transmission lines is analyzed using data from power systems on incidents with aerial transmission lines over a ten year operating period (1997-2006). It is found that failures of lightning arrester cables occur when a tensile force acts on a cable heated to the melting point by a lightning current. The lightning currents required to heat a cable to this extent are greater for larger cable cross sections. The probability that a lightning discharge will develop decreases as the amplitude of the lightning current increases, which greatly reduces the number of lightning discharges which damage TK-70 cables compared to TK-50 cables. In order to increase the reliability of lightning arrester cables for 110 kV aerial transmission lines, TK-70 cables should be used in place of TK-50 cables. The number of lightning discharges per year which damage lightning arrester cables is lowered when the density of aerial transmission lines is reduced within the territory of electrical power systems. An approximate relationship between these two parameters is obtained.

  13. Lightning location characteristics and vertical structure analysis of isolated storm cells in the TOGA COARE region

    E-Print Network [OSTI]

    Barnaby, Stephen Andrew

    1995-01-01T23:59:59.000Z

    producing storm cells, and non-lightning producing cells over the tropical oceans. The characteristics of lightning locations with respect to radar reflectivity were also examined. Lightning characteristics of flashes associated with TOGA COARE storm cells...

  14. 428 IEEE TRANSACTIONS ON ELECTROMAGNETIC COMPATIBILITY, VOL. 51, NO. 3, AUGUST 2009 Overview of Recent Progress in Lightning

    E-Print Network [OSTI]

    Florida, University of

    /lightning locating systems, light- ning electromagnetic pulse and lightning-induced effects, and protection against lightning-induced effects. Index Terms--Lightning discharge, lightning electromagnetic pulse (LEMP electromagnetic pulse (LEMP) and induced effects; 4) protection against LEMP. The first special issue on lightning

  15. ORIGINAL PAPER Development and validation of a modular, extensible docking

    E-Print Network [OSTI]

    Rizzo, Robert C.

    ORIGINAL PAPER Development and validation of a modular, extensible docking program: DOCK 5 Demetri point out that success rates could be improved through more advanced modeling of the receptor prior non-covalent interactions are critical for bio- logical processes. The sequencing of a variety of ge

  16. . DNA, RNA, Protein

    E-Print Network [OSTI]

    Yoo, SukIn

    1. , . DNA, RNA, Protein [1-4]. DNA . DNA A, T, G, C . DNA , DNA DNA . , DNA DNA . DNA DNA . , PCR , , DNA [5]. DNA DNA , DNA

  17. A Docked-Aware Storage Architecture for Mobile Computing Christopher R. LaRosa

    E-Print Network [OSTI]

    Bailey, Mark W.

    . Keywords Hoarding, file system, energy, power, battery life, caching, handheld, palmtop, docked. Permission

  18. A Docking Casette For Printed Circuit Boards

    DOE Patents [OSTI]

    Barringer, Dennis R. (Wallkill, NY); Seminaro, Edward J. (Milton, NY); Toffler, Harold M. (Newburgh, NY)

    2003-08-19T23:59:59.000Z

    A docking apparatus for printed circuit boards including a cassette housing, having a housing base, a housing cover and a housing wall, wherein the housing base and the housing wall are disposed relative to each other so as to define a housing cavity for containing a printed circuit board and wherein the housing wall includes a cable opening disposed so as to be communicated with the housing cavity, a linkage mechanism, wherein the linkage mechanism includes an engagement configuration and a disengagement configuration and wherein the linkage mechanism is disposed so as to be associated with the cassette housing and a housing bezel, wherein the housing bezel is disposed relative to the cassette housing so as to be associated with the cable opening.

  19. Broadband VLF measurements of lightning-induced ionospheric perturbations

    E-Print Network [OSTI]

    Cummer, Steven A.

    2005. [1] Very low frequency (VLF) electromagnetic pulses radiated by lightning are an effective tool with the theoretically predicted ionization changes produced directly by the lightning electromagnetic pulse. Citation over the thunderstorm due to the heating of ionospheric electrons by the electromagnetic pulse (EMP

  20. LIGHTNING EXPOSURE OF WIND TURBINES University of Toronto

    E-Print Network [OSTI]

    Lehn, Peter W.

    LIGHTNING EXPOSURE OF WIND TURBINES Dale Dolan University of Toronto e-mail: dale@ecf.utoronto.ca Abstract This paper applies the electrogeometric model of lightning exposure to a wind turbine to compute. For a typical 45 m wind turbine, the probability of being struck by a downward negative flash, as predicted

  1. An improved model of the lightning electromagnetic field interaction with the D-region ionosphere

    E-Print Network [OSTI]

    14 March 2012. [1] We present an improved time-domain model of the lightning electromagnetic pulse. Introduction [2] Lightning discharges produce both an electromagnetic pulse (EMP), due to the rapid lightningAn improved model of the lightning electromagnetic field interaction with the D-region ionosphere R

  2. 1994 Triggered Lightning Test Program: Measured responses of a reinforced concrete building under direct lightning attachments

    SciTech Connect (OSTI)

    Schnetzer, G.H.; Chael, J.; Davis, R. [Sandia National Labs., Albuquerque, NM (United States); Fisher, R.J.; Magnotti, P.J. [Army Armament Research, Development and Engineering Center, Picatinny Arsenal, NJ (United States)

    1995-08-01T23:59:59.000Z

    A rocket-triggered lightning test was carried out during the summer of 1994 on a specially designed steel reinforced concrete test building located at Ft. McClellan, Alabama. Currents, voltages, and magnetic fields were measured at 24 instrumented locations during 42 return strokes triggered to designated points on the structure and its lightning protection systems. As was found during an earlier similar lightning test of an earth covered munitions storage building, the buried power service conduits carried a much larger fraction of incident stroke current away from the building than did the intended grounding elements of the lightning protection system. Electrical breakdown and subsequent arcing occurred repeatedly to create dominant current paths to earth that were not accounted for in pretest linear modeling. Potential hazard level transient voltages, surprisingly more resistive than inductive in nature, were recorded throughout the structure. Also surprisingly, strikes to a single grounded protection mast system resulted in internal environments that were generally comparable to those occurring during strikes to roof-mounted air terminals. A description of the test structure, experimental procedures, and a full set of the resultant data are presented in this two-volume report.

  3. Autonomous Docking of a Smart Wheelchair for the

    E-Print Network [OSTI]

    Spletzer, John R.

    utility vehicle. At the core of ATRS is a "smart" wheelchair sys- tem that autonomously navigates between tank, fuel system, and heating/ cooling systems of the vehicle (Wood, 2004). While enabling independent Autonomous Docking of a Smart

  4. A genetic algorithm based method for docking flexible molecules

    SciTech Connect (OSTI)

    Judson, R.S. [Sandia National Labs., Livermore, CA (United States); Jaeger, E.P.; Treasurywala, A.M. [Sterling-Winthrop Inc., Collegeville, PA (United States)

    1993-11-01T23:59:59.000Z

    The authors describe a computational method for docking flexible molecules into protein binding sites. The method uses a genetic algorithm (GA) to search the combined conformation/orientation space of the molecule to find low energy conformation. Several techniques are described that increase the efficiency of the basic search method. These include the use of several interacting GA subpopulations or niches; the use of a growing algorithm that initially docks only a small part of the molecule; and the use of gradient minimization during the search. To illustrate the method, they dock Cbz-GlyP-Leu-Leu (ZGLL) into thermolysin. This system was chosen because a well refined crystal structure is available and because another docking method had previously been tested on this system. Their method is able to find conformations that lie physically close to and in some cases lower in energy than the crystal conformation in reasonable periods of time on readily available hardware.

  5. Demonstration Dock Designed to Benefit Eelgrass Habitat Restoration (Washington)

    SciTech Connect (OSTI)

    Diefenderfer, Heida L.; Sargeant, Susan L.; Thom, Ronald M.; Borde, Amy B.; Gayaldo, Perry F.; Curtis, Craig A.; Court, Brian L.; Pierce, David M.; Robison, David S.

    2004-06-01T23:59:59.000Z

    The derelict Thomas Oil Dock on state tidelands in Port Townsend, Washington, was redesigned for the Northwest Maritime Center by a committee including marine scientists, architects, engineers, educators, regulators, and user groups. The committee's objectives were to create a ''demonstration dock'' using the best-available technologies and design features to restore nearshore habitat functions, particularly for threatened fisheries resources, while accommodating the unique requirements of the educational facility to house vessels ranging from historic tall ships to sea kayaks. A key nearshore habitat restoration goal was to reduce fragmentation of eelgrass where shade from the existing dock inhibited growth, by improving conditions with a new dock design and transplanting eelgrass to connect existing patches. Ecological conditions were evaluated through diver surveys; mapping existing eelgrass, macroalgae, and substrates; and review of controlling factors. Data on the attenuation an d diffusion of photosynthetically-active radiation (PAR) was collected and evaluated relative to eelgrass light requirements. Potential shade impacts of existing and alternative dock designs and materials were modeled. Key design features to improve habitat functions included the installation of reflective material under the dock to increase the incidence of PAR at the substrate level, reduction in the number of piles and associated shade impacts through the use of steel piles instead of wood, increase in the length of the trestle to 286 to move the greatest area of overwater structure beyond the range of eelgrass, and the use of grating in strategic locations to reduce the potential of a light/dark barrier to fish passage.

  6. Lightning strokes can probe the ionosphere

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of Science (SC)Integrated Codes |Is Your HomeLatestCenter (LMI-EFRC) -Choices to SaveandLightning

  7. GRED STUDIES AND DRILLING OF AMERICULTURE STATE 2, AMERICULTURE TILAPIA FARM LIGHTNING DOCK KGRA, ANIMAS VALLEY, NM

    SciTech Connect (OSTI)

    Witcher, James

    2006-08-01T23:59:59.000Z

    This report summarizes the GRED drilling operations in the AmeriCulture State 2 well with an overview of the preliminary geologic and geothermal findings, from drill cuttings, core, geophysical logs and water geochemical sampling.

  8. Lightning and radar observations of hurricane Rita landfall

    SciTech Connect (OSTI)

    Henderson, Bradley G [Los Alamos National Laboratory; Suszcynsky, David M [Los Alamos National Laboratory; Hamlin, Timothy E [Los Alamos National Laboratory; Jeffery, C A [Los Alamos National Laboratory; Wiens, Kyle C [TEXAS TECH U.; Orville, R E [TEXAS A& M

    2009-01-01T23:59:59.000Z

    Los Alamos National Laboratory (LANL) owns and operates an array of Very-Low Frequency (VLF) sensors that measure the Radio-Frequency (RF) waveforms emitted by Cloud-to-Ground (CG) and InCloud (IC) lightning. This array, the Los Alamos Sferic Array (LASA), has approximately 15 sensors concentrated in the Great Plains and Florida, which detect electric field changes in a bandwidth from 200 Hz to 500 kHz (Smith et al., 2002). Recently, LANL has begun development of a new dual-band RF sensor array that includes the Very-High Frequency (VHF) band as well as the VLF. Whereas VLF lightning emissions can be used to deduce physical parameters such as lightning type and peak current, VHF emissions can be used to perform precise 3d mapping of individual radiation sources, which can number in the thousands for a typical CG flash. These new dual-band sensors will be used to monitor lightning activity in hurricanes in an effort to better predict intensification cycles. Although the new LANL dual-band array is not yet operational, we have begun initial work utilizing both VLF and VHF lightning data to monitor hurricane evolution. In this paper, we present the temporal evolution of Rita's landfall using VLF and VHF lightning data, and also WSR-88D radar. At landfall, Rita's northern eyewall experienced strong updrafts and significant lightning activity that appear to mark a transition between oceanic hurricane dynamics and continental thunderstorm dynamics. In section 2, we give a brief overview of Hurricane Rita, including its development as a hurricane and its lightning history. In the following section, we present WSR-88D data of Rita's landfall, including reflectivity images and temporal variation. In section 4, we present both VHF and VLF lightning data, overplotted on radar reflectivity images. Finally, we discuss our observations, including a comparison to previous studies and a brief conclusion.

  9. Experts are partnering in Tampa, the Lightning Capital of the U.S., to provide safety information about the dangers of lightning and promote

    E-Print Network [OSTI]

    this free event to learn more about the national Lightning Safety Awareness Campaign to protect your family about the dangers of lightning and promote risk reduction and lightning protection education. Attend safety & protection resources FEATURED GUESTS INCLUDE: · Tampa Mayor Bob Buckhorn · Tampa Fire Chief

  10. analyzing lightning field: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    A roughly second order power-law fit to the mean radar echo top height above the 0C isotherm is shown to capture both regional and land-ocean contrasts in lightning...

  11. Local lightning early warning system (LLEWS), Phase 1

    SciTech Connect (OSTI)

    Fisher, R.J.

    1992-12-01T23:59:59.000Z

    Results are presented of a detailed survey of the present state of scientific understanding of cloud electrification processes and lightning warning technology. A review is given of the principles of operation and demonstrated performance factors of lightning strike location technologies and associated commercial products. Emphasis is given to the local lightning warning problem, which is divided into two categories: detection and tracking of active storms that originate outside of and move into the declared safety zone, and early detection of cloud electrification that initiates within the zone. A prototype single-station warning system design is presented that is intended to accumulate data simultaneously from a complement of different types of sensors during intervals immediately preceding the onset of lightning conditions within the area of coverage. The resultant data base will be analyzed statistically to identify the most promising combinations of early warning indicators and to quantify their reliability as a function of the warning intervals they provide.

  12. GRAMM-X public web server for protein-protein docking

    E-Print Network [OSTI]

    Tovchigrechko, Andrey; Vakser, Ilya A.

    2006-07-01T23:59:59.000Z

    -based scoring. The web server frees users from complex installation of database-dependent parallel software and maintaining large hardware resources needed for protein docking simulations. Docking problems submitted to GRAMM-X server are processed by a 320...

  13. Sampling the conformation of protein surface residues for flexible protein docking

    E-Print Network [OSTI]

    Francis-Lyon, Patricia; Gu, Shengyin; Hass, Joel; Amenta, Nina; Koehl, Patrice

    2010-01-01T23:59:59.000Z

    References 1. Bonvin AM: Flexible protein-protein docking.Wolfson H: Principles of flexible protein-protein docking.R, Wolfson HJ: Geometry-based flexible and symmetric protein

  14. X-ray Emission from Thunderstorms and Lightning

    ScienceCinema (OSTI)

    Joseph Dwyer

    2010-01-08T23:59:59.000Z

    How lightning is initiated in the relatively low electric fields inside thunderclouds and how it can then propagate for tens of kilometers through virgin air are two of the great unsolved problems in the atmospheric sciences. Until very recently it was believed that lightning was entirely a conventional discharge, involving only low-energy (a few eV) electrons. This picture changed completely a few years ago with the discovery of intense x-ray emission from both natural cloud-to-ground lightning and rocket-triggered lightning. This energetic emission cannot be produced by a conventional discharge, and so the presence of x-rays strongly implies that runaway breakdown plays a role in lightning processes. During runaway breakdown, electrons are accelerated through air to nearly the speed of light by strong electric fields. These runaway electrons then emit bremsstrahlung x-rays and gamma-rays during collisions with air. Indeed, the x-ray and gamma-ray emission produced by runaway breakdown near the tops of thunderstorms is bright enough to be seen from outer space, 600 km away. As a result, the physics used for decades to describe thunderstorm electrification and lightning discharges is incomplete and needs to be revisited.

  15. DARS (Decoys As the Reference State) Potentials for Protein-Protein Docking

    E-Print Network [OSTI]

    Vajda, Sandor

    DARS (Decoys As the Reference State) Potentials for Protein-Protein Docking Gwo-Yu Chuang,* Dima As the Reference State (DARS) is a simple and natural approach to the construction of structure directly in docking calculations. We investigated the performance of various DARS versions for docking

  16. Variability of CONUS Lightning in 200312 and Associated Impacts

    E-Print Network [OSTI]

    Koshak, William J.

    Changes in lightning characteristics over the conterminous United States (CONUS) are examined to support the National Climate Assessment (NCA) program. Details of the variability of cloud-to-ground (CG) lightning characteristics ...

  17. A possible relationship between Global Warming and Lightning Activity in India during the period 1998-2009

    E-Print Network [OSTI]

    Felix Pereira B.; Priyadarsini G.; T. E. Girish

    2010-12-15T23:59:59.000Z

    Lightning activity on a global scale has been studied season wise using satellite data for the period from 1998 to 2009. Lightning activity shows an increasing trend during the period of study which is highly correlated with atmospheric warming. A similar increasing trend of lightning activity is observed in the Indian region during the pre-monsoon season which is correlated with global lightning trends and warming trends of surface temperature in India. Key words: Global warming, lightning activity, Solar cycle changes

  18. Total lightning observations of severe convection over North Texas

    E-Print Network [OSTI]

    McKinney, Christopher Michael

    2009-05-15T23:59:59.000Z

    Density GSD Gridded Source Density LDAR Lightning Detection and Ranging MCS Mesoscale Convective System MSI Mesocyclone Strength Index MxFED Maximum Flash Extent Density MxFIDT Maximum Flash Initiation Density Total MxGSD Maximum Gridded Source.......................................................................................... 1 1.2 Background ....................................................................................... 4 1.3 Thesis Objectives and Hypothesis...................................................... 19 2. DATA AND METHODOLOGY...

  19. Radar Nowcasting of Total Lightning over the Kennedy Space Center

    E-Print Network [OSTI]

    Seroka, Gregory Nicholas

    2011-08-08T23:59:59.000Z

    BZ at -20 degrees C, while the best reflectivity at isothermal predictor for IC was 25 dBZ at -15 degrees C. Meanwhile, the best VII predictor of CG lightning was the 30th percentile (0.840 kg m-2), while the best VII predictor of IC was the 5th percentile...

  20. On the transmission line model for lightning return stroke representation

    E-Print Network [OSTI]

    Florida, University of

    [Uman and McLain, 1969] has been widely used for lightning electromagnetic pulse (LEMP) calculations of current sources that produce a spherical transverse electromagnetic (TEM) wave in the case of return electromagnetic field structure is non-TEM. INDEX TERMS: 0619 Electromagnetics: Electromagnetic theory; 0684

  1. Characterization of infrasound from lightning J. D. Assink,1

    E-Print Network [OSTI]

    Utrecht, Universiteit

    , high-altitude discharges [Farges et al., 2005; Liszka and Hobara, 2006]. This article will discuss CC] During thunderstorm activity in the Netherlands, electromagnetic and infrasonic signals are emitted due a electromagnetic lightning detection network is successful up to distances of 50 km from the infrasound array

  2. Carbon dioxide emission during forest fires ignited by lightning

    E-Print Network [OSTI]

    Magdalena Pelc; Radoslaw Osuch

    2009-03-31T23:59:59.000Z

    In this paper we developed the model for the carbon dioxide emission from forest fire. The master equation for the spreading of the carbon dioxide to atmosphere is the hyperbolic diffusion equation. In the paper we study forest fire ignited by lightning. In that case the fores fire has the well defined front which propagates with finite velocity.

  3. ProteinProtein Docking with Backbone Flexibility Chu Wang, Philip Bradley and David Baker

    E-Print Network [OSTI]

    Baker, David

    ProteinProtein Docking with Backbone Flexibility Chu Wang, Philip Bradley and David Baker Department of Biochemistry and Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195

  4. DOCK2 regulates cell proliferation through Rac and ERK activation in B cell lymphoma

    SciTech Connect (OSTI)

    Wang, Lei [Laboratory of Cancer Research, Department of Pathology, Hokkaido University Graduate School of Medicine, N15W7, Kitaku, Sapporo 060-8638 (Japan)] [Laboratory of Cancer Research, Department of Pathology, Hokkaido University Graduate School of Medicine, N15W7, Kitaku, Sapporo 060-8638 (Japan); Nishihara, Hiroshi, E-mail: nisihara@patho2.med.hokudai.ac.jp [Laboratory of Translational Pathology, Department of Pathology, Hokkaido University Graduate School of Medicine, N15W7, Kitaku, Sapporo 060-8638 (Japan)] [Laboratory of Translational Pathology, Department of Pathology, Hokkaido University Graduate School of Medicine, N15W7, Kitaku, Sapporo 060-8638 (Japan); Kimura, Taichi; Kato, Yasutaka; Tanino, Mishie [Laboratory of Cancer Research, Department of Pathology, Hokkaido University Graduate School of Medicine, N15W7, Kitaku, Sapporo 060-8638 (Japan)] [Laboratory of Cancer Research, Department of Pathology, Hokkaido University Graduate School of Medicine, N15W7, Kitaku, Sapporo 060-8638 (Japan); Nishio, Mitsufumi; Obara, Masato; Endo, Tomoyuki; Koike, Takao [Department of Medicine II, Hokkaido University Graduate School of Medicine, N15W7, Kitaku, Sapporo 060-8638 (Japan)] [Department of Medicine II, Hokkaido University Graduate School of Medicine, N15W7, Kitaku, Sapporo 060-8638 (Japan); Tanaka, Shinya [Laboratory of Cancer Research, Department of Pathology, Hokkaido University Graduate School of Medicine, N15W7, Kitaku, Sapporo 060-8638 (Japan)] [Laboratory of Cancer Research, Department of Pathology, Hokkaido University Graduate School of Medicine, N15W7, Kitaku, Sapporo 060-8638 (Japan)

    2010-04-23T23:59:59.000Z

    DOCK2; a member of the CDM protein family, regulates cell motility and cytokine production through the activation of Rac in mammalian hematopoietic cells and plays a pivotal role in the modulation of the immune system. Here we demonstrated the alternative function of DOCK2 in hematopoietic tumor cells, especially in terms of its association with the tumor progression. Immunostaining for DOCK2 in 20 cases of human B cell lymphoma tissue specimens including diffuse large B cell lymphoma and follicular lymphoma revealed the prominent expression of DOCK2 in all of the lymphoma cells. DOCK2-knockdown (KD) of the B cell lymphoma cell lines, Ramos and Raji, using the lentiviral shRNA system presented decreased cell proliferation compared to the control cells. Furthermore, the tumor formation of DOCK2-KD Ramos cell in nude mice was significantly abrogated. Western blotting analysis and pull-down assay using GST-PAK-RBD kimeric protein suggested the presence of DOCK2-Rac-ERK pathway regulating the cell proliferation of these lymphoma cells. This is the first report to clarify the prominent role of DOCK2 in hematopoietic malignancy.

  5. Computer Graphics in Real-time Docking with Energy Calculation and Minimization

    E-Print Network [OSTI]

    Levitt, Michael

    Computer Graphics in Real-time Docking with Energy Calculation and Minimization N. Pattabiraman, M. Levitt*, T.E. Ferrin, R. Langridge Computer Graphics Laboratory, Department of Pharmaceutical Chemistry 1985 We describe a real-time docking method using molecular graphics and high-speed calculation

  6. Scheduling incoming and outgoing trucks at cross docking terminals to minimize the storage cost

    E-Print Network [OSTI]

    Sadykov, Ruslan

    Scheduling incoming and outgoing trucks at cross docking terminals to minimize the storage cost Ruslan Sadykov June 14, 2012 Abstract Cross docking terminals allow companies to reduce storage and trans- portation costs in a supply chain. At these terminals, products of different types from incoming trucks

  7. Nanotechnology with DNA DNA Nanodevices

    E-Print Network [OSTI]

    Ludwig-Maximilians-Universitt, Mnchen

    Nanotechnology with DNA DNA Nanodevices Friedrich C. Simmel* and Wendy U. Dittmer A DNA actuator. Introduction.............285 2. Overview: DNA Nanotechnology.......285 3. Prototypes of Nanomechanical DNA overview of DNA nanotechnology as a whole is given. The most important properties of DNA molecules

  8. Low-level convergence and its role in convective intensity and frequency over the Houston lightning and rainfall anomaly

    E-Print Network [OSTI]

    McNear, Veronica Ann

    2007-09-17T23:59:59.000Z

    . By using these images along with average lightning, rainfall, and reflectivity for a large Houston-centered domain, it was possible to discern a correlation between low-level convergence and convection. Also, past findings of enhancement in lightning...

  9. DNA microarray (spot) .

    E-Print Network [OSTI]

    1. DNA microarray DNA (spot) . DNA probe , probe (hybridization) . DNA microarray cDNA oligonucleotide oligonucleotide cDNA probe . oligonucleotide microarray , DNA , probe . oligonucleotide microarray probe

  10. A ground level gamma-ray burst observed in association with rocket-triggered lightning

    E-Print Network [OSTI]

    Florida, University of

    A ground level gamma-ray burst observed in association with rocket-triggered lightning J. R. Dwyer 2004; published 13 March 2004. [1] We report the observation of an intense gamma-ray burst observed lightning channel with gamma-ray energies extending up to more than 10 MeV. The burst consisted of 227

  11. Temporal-spatial modeling of electron density enhancement due to successive lightning strokes

    E-Print Network [OSTI]

    Otago, University of

    (FDTD) model that describes the effect of lightning electromagnetic pulses (EMP) on the ionosphere. Each The electromagnetic pulse (EMP) from lightning has been shown to modify conductivity and electron density in the lower successive EMP pulse interacts with a modified background ionosphere due to the previous pulses, resulting

  12. Thunderstorm lightning and radar characteristics: insights on electrification and severe weather forecasting

    E-Print Network [OSTI]

    Steiger, Scott Michael

    2007-04-25T23:59:59.000Z

    THUNDERSTORM LIGHTNING AND RADAR CHARACTERISTICS: INSIGHTS ON ELECTRIFICATION AND SEVERE WEATHER FORECASTING A Dissertation by SCOTT MICHAEL STEIGER Submitted to the Office of Graduate Studies of Texas A&M University in partial... fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY December 2005 Major Subject: Atmospheric Sciences THUNDERSTORM LIGHTNING AND RADAR CHARACTERISTICS: INSIGHTS ON ELECTRIFICATION AND SEVERE WEATHER...

  13. Source altitudes of terrestrial gamma-ray flashes produced by lightning leaders

    E-Print Network [OSTI]

    Pasko, Victor

    ; published 18 April 2012. [1] Terrestrial gamma-ray flashes (TGFs) are energetic photon bursts observed fromSource altitudes of terrestrial gamma-ray flashes produced by lightning leaders Wei Xu,1 Sebastien. Pasko (2012), Source altitudes of terres- trial gamma-ray flashes produced by lightning leaders, Geophys

  14. A stepped leader model for lightning including charge distribution in branched channels

    SciTech Connect (OSTI)

    Shi, Wei; Zhang, Li [School of Electrical Engineering, Shandong University, Jinan 250061 (China); Li, Qingmin, E-mail: lqmeee@ncepu.edu.cn [Beijing Key Lab of HV and EMC, North China Electric Power University, Beijing 102206 (China); State Key Lab of Alternate Electrical Power System with Renewable Energy Sources, Beijing 102206 (China)

    2014-09-14T23:59:59.000Z

    The stepped leader process in negative cloud-to-ground lightning plays a vital role in lightning protection analysis. As lightning discharge usually presents significant branched or tortuous channels, the charge distribution along the branched channels and the stochastic feature of stepped leader propagation were investigated in this paper. The charge density along the leader channel and the charge in the leader tip for each lightning branch were approximated by introducing branch correlation coefficients. In combination with geometric characteristics of natural lightning discharge, a stochastic stepped leader propagation model was presented based on the fractal theory. By comparing simulation results with the statistics of natural lightning discharges, it was found that the fractal dimension of lightning trajectory in simulation was in the range of that observed in nature and the calculation results of electric field at ground level were in good agreement with the measurements of a negative flash, which shows the validity of this proposed model. Furthermore, a new equation to estimate the lightning striking distance to flat ground was suggested based on the present model. The striking distance obtained by this new equation is smaller than the value estimated by previous equations, which indicates that the traditional equations may somewhat overestimate the attractive effect of the ground.

  15. The effects of lightning and high altitude electromagnetic pulse on power distribution lines

    SciTech Connect (OSTI)

    Uman, M.A.; Rubinstein, M.; Yacoub, Z. [Florida Univ., Gainesville, FL (United States)

    1995-01-01T23:59:59.000Z

    We simultaneously recorded the voltages induced by lightning on both ends of an unenergized 448-meter long unenergized electric power line and the lightning vertical electric and horizontal magnetic fields at ground level near the line. The lightning data studied and presented here were due both to cloud lightning and to very close (about 20 m from the line) artificially initiated lightning. For cloud sources, a frequency-domain computer program called EMPLIN was used to calculate induced line voltages as a function of source elevation, angle of incidence, and wave polarization of the radiated cloud discharge pulses in order to compare with the measurements. For very-close lightning, the measured line voltages could be grouped into two categories, those in which multiple, similarly shaped, evenly spaced pulses were observed, which we call oscillatory, and those dominated by a principal pulse with subsidiary oscillations of much smaller amplitude, which we call impulsive. The amplitude of the induced voltage ranged from tens of kilovolts for oscillatory voltages to hundreds of kilovolts for impulsive voltages. A new technique is derived for the calculation of the electromagnetic fields from nearby lightning to ground above an imperfectly conducting ground. This technique was used in conjunction with an existing time domain coupling theory and lightning return stroke model to calculate voltages at either end of the line. The results show fair agreement with the measured oscillatory voltage waveforms if corona is ignored and improved results when corona effects are modeled. The modeling of the impulsive voltage, for which local flashover probably successful. In an attempt to understand better the sources of the line voltages for very close lightning, measurements of the horizontal and vertical electric fields 30 m from triggered lightning were obtained.

  16. Update Direct-Strike Lightning Environment for Stockpile-to-Target Sequence

    SciTech Connect (OSTI)

    Uman, M A; Rakov, V A; Elisme, J O; Jordan, D M; Biagi, C J; Hill, J D

    2008-10-01T23:59:59.000Z

    The University of Florida has surveyed all relevant publications reporting lightning characteristics and presents here an up-to-date version of the direct-strike lightning environment specifications for nuclear weapons published in 1989 by R. J. Fisher and M. A. Uman. Further, we present functional expressions for current vs. time, current derivative vs. time, second current derivative vs. time, charge transfer vs. time, and action integral (specific energy) vs. time for first return strokes, for subsequent return strokes, and for continuing currents; and we give sets of constants for these expressions so that they yield approximately the median and extreme negative lightning parameters presented in this report. Expressions for the median negative lightning waveforms are plotted. Finally, we provide information on direct-strike lightning damage to metals such as stainless steel, which could be used as components of storage containers for nuclear waste materials; and we describe UF's new experimental research program to add to the sparse data base on the properties of positive lightning. Our literature survey, referred to above, is included in four Appendices. The following four sections (II, III, IV, and V) of this final report deal with related aspects of the research: Section II. Recommended Direct-Strike Median and Extreme Parameters; Section III. Time-Domain Waveforms for First Strokes, Subsequent Strokes, and Continuing Currents; Section IV. Damage to Metal Surfaces by Lightning Currents; and Section V. Measurement of the Characteristics of Positive Lightning. Results of the literature search used to derive the material in Section II and Section IV are found in the Appendices: Appendix 1. Return Stroke Current, Appendix 2. Continuing Current, Appendix 3. Positive Lightning, and Appendix 4. Lightning Damage to Metal Surfaces.

  17. DNA Computing Hamiltonian path

    E-Print Network [OSTI]

    Hagiya, Masami

    2014 DNA DNA #12;DNA Computing Feynman Adleman DNASIMD ... DNADNA #12;DNA DNA DNA #12;2000 2005 2010 1995 Hamiltonian path DNA tweezers DNA tile DNA origami DNA box Sierpinski DNA tile self assembly DNA logic gates Whiplash PCR DNA automaton DNA spider MAYA

  18. A radar study of the interaction between lightning and precipitation

    SciTech Connect (OSTI)

    Holden, D.N.; Ulbrich, C.W.

    1988-01-01T23:59:59.000Z

    A radar study was made of the interaction between lightning and precipitation with the 430 MHz Doppler radar at the Arecibo Observatory in Puerto Rico. On one occasion, the spectral power at Doppler velocities near that corresponding to the updraft increased substantially within a fraction of a second after a discharge was detected in the beam. Calculations were made to simulate the effect of an electric field change on mean Doppler velocity for a distribution of droplets in a thunderstorm. 13 refs., 4 figs.

  19. Evaluation of an approximate method for incorporating floating docks in harbor wave prediction models

    E-Print Network [OSTI]

    Tang, Zhaoxiang

    2005-11-01T23:59:59.000Z

    coastal domains. However, floating structures such as floating breakwaters and docks are often encountered in the modeling domain. This makes the problem locally 3- dimensional. Hence it is problematic to incorporate a floating structure into the 2-d model...

  20. Protein Docking by the Interface Structure Similarity: How Much Structure Is Needed?

    E-Print Network [OSTI]

    Sinha, Rohita; Kundrotas, Petras J.; Vakser, Ilya A.

    2012-02-13T23:59:59.000Z

    The increasing availability of co-crystallized protein-protein complexes provides an opportunity to use template-based modeling for protein-protein docking. Structure alignment techniques are useful in detection of remote ...

  1. Optimal location of cross-docking centers for a distribution network in Argentina

    E-Print Network [OSTI]

    Dobrusky, Fernando G. (Fernando Gabriel), 1971-

    2003-01-01T23:59:59.000Z

    The objective of this thesis is to define an optimal distribution network for Argentina using Cross Docking Centers. The products to be delivered are in a Central Warehouse in Buenos Aires, the main city and port of ...

  2. Connection stiffness and dynamical docking process of flux pinned spacecraft modules

    SciTech Connect (OSTI)

    Lu, Yong; Zhang, Mingliang, E-mail: niudun12@126.com; Gao, Dong [School of Mechatronics Engineering, Harbin Institute of Technology, Harbin, Heilongjiang 150001 (China)

    2014-02-14T23:59:59.000Z

    This paper describes a novel kind of potential flux pinned docking system that consists of guidance navigation and control system, the traditional extrusion type propulsion system, and a flux pinned docking interface. Because of characteristics of passive stability of flux pinning, the docking control strategy of flux pinned docking system only needs a series of sequential control rather than necessary active feedback control, as well as avoidance of hazardous collision accident. The flux pinned force between YBaCuO (YBCO) high temperature superconductor bulk and permanent magnet is able to be given vent based on the identical current loop model and improved image dipole model, which can be validated experimentally. Thus, the connection stiffness between two flux pinned spacecraft modules can be calculated based on Hooke's law. This connection stiffness matrix at the equilibrium position has the positive definite performance, which can validate the passively stable connection of two flux pinned spacecraft modules theoretically. Furthermore, the relative orbital dynamical equation of two flux pinned spacecraft modules can be established based on Clohessy-Wiltshire's equations and improved image dipole model. The dynamical docking process between two flux pinned spacecraft modules can be obtained by way of numerical simulation, which suggests the feasibility of flux pinned docking system.

  3. Packaging Waste and Hitting Home Runs: How Education and Lightning Strike Detection Technology Supports Company and Community Activities

    SciTech Connect (OSTI)

    Deecke, T.A. [Weston Solutions, Inc., 297 Kentucky Avenue, Kevil, KY 42053 (United States); Hyde, J.V.; Hylko, J.M. [WESKEM, LLC, 297 Kentucky Avenue, Kevil, KY 42053 (United States)

    2006-07-01T23:59:59.000Z

    The weather is the most significant and unmanageable variable when performing environmental remediation activities. This variable can contribute to the failure of a project in two ways: 1) severe injury to an employee or employees following a cloud-to-ground lightning strike without prior visual or audible warnings; and 2) excessive 'down time' associated with mobilization and demobilization activities after a false alarm (e.g., lightning was seen in the distance but was actually moving away from the site). Therefore, in order for a project to be successful from both safety and financial viewpoints, the uncertainties associated with inclement weather, specifically lightning, need to be understood to eliminate the element of surprise. This paper discusses educational information related to the history and research of lightning, how lightning storms develop, types of lightning, the mechanisms of lightning injuries and fatalities, and follow-up medical treatment. Fortunately, lightning storm monitoring does not have to be either costly or elaborate. WESKEM, LLC selected the Boltek StormTracker Lightning Detection System with the Aninoquisi Lightning 2000{sup TM} software. This fixed system, used in combination with online weather web pages, monitors and alarms WESKEM, LLC field personnel in the event of an approaching lightning storm. This application was expanded to justify the purchase of the hand-held Sky Scan Lightning/Storm Detector Model P5 used by the Heath Youth Athletic Association (HYAA) which is a non-profit, charitable organization offering sports programs for the youth and young adults in the local community. Fortunately, a lightning injury or fatality has never occurred on a WESKEM Paducah project or an HYAA-sponsored event. Using these fixed and hand-held systems will continue to prevent such injuries from occurring in the foreseeable future. (authors)

  4. Crusader Automated Docking System: Technology support for the Crusader Resupply Team. Interim report, Ammunition Logistics Program

    SciTech Connect (OSTI)

    Kring, C.T.; Varma, V.K.; Jatko, W.B.

    1995-11-01T23:59:59.000Z

    The US Army and Team Crusader (United Defense, Lockheed Martin Armament Systems, etc.) are developing the next generation howitzer, the Crusader. The development program includes an advanced, self-propelled liquid propellant howitzer and a companion resupply vehicle. The resupply vehicle is intended to rendezvous with the howitzer near the battlefront and replenish ammunition, fuel, and other material. The Army has recommended that Crusader incorporate new and innovative technologies to improve performance and safety. One conceptual design proposes a robotic resupply boom on the resupply vehicle to upload supplies to the howitzer. The resupply boom would normally be retracted inside the resupply vehicle during transit. When the two vehicles are within range of the resupply boom, the boom would be extended to a receiving port on the howitzer. In order to reduce exposure to small arms fire or nuclear, biological, and chemical hazards, the crew would remain inside the resupply vehicle during the resupply operation. The process of extending the boom and linking with the receiving port is called docking. A boom operator would be designated to maneuver the boom into contact with the receiving port using a mechanical joystick. The docking operation depends greatly upon the skill of the boom operator to manipulate the boom into docking position. Computer simulations at the National Aeronautics and Space Administration have shown that computer-assisted or autonomous docking can improve the ability of the operator to dock safely and quickly. This document describes the present status of the Crusader Autonomous Docking System (CADS) implemented at Oak Ridge National laboratory (ORNL). The purpose of the CADS project is to determine the feasibility and performance limitations of vision systems to satisfy the autonomous docking requirements for Crusader and conduct a demonstration under controlled conditions.

  5. Long recovery VLF perturbations associated with lightning M. M. Salut,1

    E-Print Network [OSTI]

    .1029/2012JA017567. 1. Introduction [2] The intense electromagnetic pulse (EMP) radiated from powerful cloud with direct coupling of the lightning electromagnetic energy to the lower ionosphere. Also, it has been shown

  6. Radar and lightning analyses of gigantic jet-producing storms Tiffany C. Meyer,1,2

    E-Print Network [OSTI]

    Rutledge, Steven

    Radar and lightning analyses of gigantic jet-producing storms Tiffany C. Meyer,1,2 Timothy J. Lang of gigantic jets, similar to prior modeling studies. Citation: Meyer, T. C., T. J. Lang, S. A. Rutledge, W. A

  7. The warning time for cloud-to-ground lightning in isolated, ordinary thunderstorms over Houston, Texas

    E-Print Network [OSTI]

    Clements, Nathan Chase

    2009-05-15T23:59:59.000Z

    electrical circuit. The thunderstorm is efectively a batery, charging the earth?s surface with negative charge lowered due to lightning discharges. Altitudes are not to scale........................................................8 3 Positive dipole... circuit. The thunderstorm is efectively a batery, charging the earth?s surface with negative charge lowered due to lightning discharges. Altitudes are not to scale (From MacGorman and Rust 1998, p. 31). 9 To determine...

  8. Docking HPr to HPr Kinase with Partial Backbone Flexibility A thesis submitted to the Johns Hopkins University in conformity with the

    E-Print Network [OSTI]

    Gray, Jeffrey J.

    Docking HPr to HPr Kinase with Partial Backbone Flexibility By Lian Guo A thesis submitted Assessment of Prediction of Interactions, a blind test of protein docking) challenge. Here, a method

  9. Highly Compliant and Self-Tightening Docking Modules for Precise and Fast Connection of Self-Reconfigurable Robots

    E-Print Network [OSTI]

    Shen, Wei-Min

    -And-Self-Tightening (CAST) developed as an effective and efficient connector for joining and releasing modules of self using no additional energy for docking and negligible amount of energy for undocking. Development in order to make the process feasible and reliable. For example, docking among locomotives and railroad

  10. DNA Copyright

    E-Print Network [OSTI]

    Torrance, Andrew W.

    2011-01-01T23:59:59.000Z

    of architecture and computer software. Sequences of DNA should also be acknowledged as eligible for copyright protection. Unaltered genomic DNA sequences would seem poor candidates for copyright protection. The case is stronger for copyright protection...

  11. DNA demethylation by DNA repair

    E-Print Network [OSTI]

    Gehring, Mary

    Active DNA demethylation underlies key facets of reproduction in flowering plants and mammals and serves a general genome housekeeping function in plants. A family of 5-methylcytosine DNA glycosylases catalyzes plant ...

  12. P1.15 DEVELOPING A STATISTICAL SCHEME TO PREDICT THE OCCURRENCE OF LIGHTNING IN SOUTH FLORIDA

    E-Print Network [OSTI]

    Fuelberg, Henry

    Cloud-to-ground (CG) lightning is a dangerous and potentially deadly natural phenomenon. Electrical systems are particularly susceptible to lightning damage. Not only are power outages disruptive to customers, but they are costly to electric providers if not repaired in a timely manner. Improved CG

  13. 532 IEEE TRANSACTIONS ON ELECTROMAGNETIC COMPATIBILITY, VOL. 51, NO. 3, AUGUST 2009 Lightning Electromagnetic Field Coupling to

    E-Print Network [OSTI]

    Florida, University of

    of both the incident lightning electromagnetic pulse (LEMP) and the effects of coupling of this field- mental validation using: 1) reduced-scale setups with LEMP and nuclear electromagnetic pulse (NEMP532 IEEE TRANSACTIONS ON ELECTROMAGNETIC COMPATIBILITY, VOL. 51, NO. 3, AUGUST 2009 Lightning

  14. PIPER: An FFT-Based Protein Docking Program with Pairwise Potentials

    E-Print Network [OSTI]

    Vajda, Sandor

    intermolecular potentials. The DARS (Decoys As the Reference State) potentials are extracted from structures distributions in the reference state. The current version of the DARS potential works well for enzymeinhibitor complexes. With the new FFT-based program, DARS provides much better docking results than the earlier

  15. Protection characteristics of a Faraday cage compromised by lightning burnthrough.

    SciTech Connect (OSTI)

    Warne, Larry Kevin; Bystrom, Edward; Jorgenson, Roy Eberhardt; Montoya, Sandra L.; Merewether, Kimball O.; Coats, Rebecca Sue; Martinez, Leonard E.; Jojola, John M.

    2012-01-01T23:59:59.000Z

    A lightning flash consists of multiple, high-amplitude but short duration return strokes. Between the return strokes is a lower amplitude, continuing current which flows for longer duration. If the walls of a Faraday cage are made of thin enough metal, the continuing current can melt a hole through the metal in a process called burnthrough. A subsequent return stroke can couple energy through this newly-formed hole. This LDRD is a study of the protection provided by a Faraday cage when it has been compromised by burnthrough. We initially repeated some previous experiments and expanded on them in terms of scope and diagnostics to form a knowledge baseline of the coupling phenomena. We then used a combination of experiment, analysis and numerical modeling to study four coupling mechanisms: indirect electric field coupling, indirect magnetic field coupling, conduction through plasma and breakdown through the hole. We discovered voltages higher than those encountered in the previous set of experiments (on the order of several hundreds of volts).

  16. DNA Pendant

    E-Print Network [OSTI]

    Hacker, Randi; Tsutsui, William

    2007-11-14T23:59:59.000Z

    Broadcast Transcript: It's a symbol of commitment. It's a memento mori. It's the DNA pendant offered by Japan's Eiwa Industry and it's two, two, two things in one. Using genetic extraction, Eiwa removes the DNA from, say, a strand of hair or a...

  17. Lightning arrestor connector lead magnesium niobate qualification pellet test procedures.

    SciTech Connect (OSTI)

    Tuohig, W. (Honeywell FM& T, Kansas City, MO); Mahoney, Patrick A.; Tuttle, Bruce Andrew; Wheeler, Jill Susanne

    2009-02-01T23:59:59.000Z

    Enhanced knowledge preservation for DOE DP technical component activities has recently received much attention. As part of this recent knowledge preservation effort, improved documentation of the sample preparation and electrical testing procedures for lead magnesium niobate--lead titanate (PMN/PT) qualification pellets was completed. The qualification pellets are fabricated from the same parent powders used to produce PMN/PT lightning arrestor connector (LAC) granules at HWF&T. In our report, the procedures for fired pellet surface preparation, electrode deposition, electrical testing and data recording are described. The dielectric measurements described in our report are an information only test. Technical reasons for selecting the electrode material, electrode size and geometry are presented. The electrical testing is based on measuring the dielectric constant and dissipation factor of the pellet during cooling from 280 C to 220 C. The most important data are the temperature for which the peak dielectric constant occurs (Curie Point temperature) and the peak dielectric constant magnitude. We determined that the peak dielectric constant for our procedure would be that measured at 1 kHz at the Curie Point. Both the peak dielectric constant and the Curie point parameters provide semi-quantitative information concerning the chemical and microstructural homogeneity of the parent material used for the production of PMN/PT granules for LACs. Finally, we have proposed flag limits for the dielectric data for the pellets. Specifically, if the temperature of the peak dielectric constant falls outside the range of 250 C {+-} 30 C we propose that a flag limit be imposed that will initiate communication between production agency and design agency personnel. If the peak dielectric constant measured falls outside the range 25,000 {+-} 10,000 we also propose that a flag limit be imposed.

  18. Development of a guidance, navigation and control architecture and validation process enabling autonomous docking to a tumbling satellite

    E-Print Network [OSTI]

    Nolet, Simon, 1975-

    2007-01-01T23:59:59.000Z

    The capability to routinely perform autonomous docking is a key enabling technology for future space exploration, as well as assembly and servicing missions for spacecraft and commercial satellites. Particularly, in more ...

  19. Physical Modeling of the Motions of a Container Ship Moored to a Dock with Comparison to Numerical Simulation

    E-Print Network [OSTI]

    Zhi, Yuanzhe

    2013-07-11T23:59:59.000Z

    model experimental results of solid dock are also compared with the numerical simulation. These comparisons indicate that the motion characteristics of the model container ship represent similar trends for both rotations and translations...

  20. Measurements and implications of the relationship between lightning and terrestrial gamma ray flashes

    E-Print Network [OSTI]

    Cummer, Steven A.

    the discovery of short bursts of gamma rays originating from Earth, called terrestrial gamma ray flashes (TGFsMeasurements and implications of the relationship between lightning and terrestrial gamma ray associated with 26 terrestrial gamma ray flashes (TGFs) recorded by the RHESSI satellite over the Caribbean

  1. A study of the lightning channel corona sheath Grzegorz Maslowski1,2

    E-Print Network [OSTI]

    Florida, University of

    ] Dynamics of lightning channel corona sheath surrounding thin channel core is examined on the basis of three. As a result, the leader channel consists of a thin core surrounded by a radially formed corona sheath. The corona sheath expands outward from the channel core until the radial electric field is less than

  2. Rare measurements of a sprite with halo event driven by a negative lightning discharge over Argentina

    E-Print Network [OSTI]

    Thomas, Jeremy N.

    Argentina M. J. Taylor,1 M. A. Bailey,1 P. D. Pautet,1 S. A. Cummer,2 N. Jaugey,2 J. N. Thomas,3,4 N. N measurements were made of a mesoscale thunderstorm observed on February 22­23, 2006 over northern Argentina of a sprite with halo event driven by a negative lightning discharge over Argentina, Geophys. Res. Lett., 35

  3. The use of Doppler radar to predict cloud-to-ground lightning

    E-Print Network [OSTI]

    Aclin, Keith Andrew

    1995-01-01T23:59:59.000Z

    during the spring of 1993 for squall line activity. These data will then be combined with the cloud-to-ground lightning that occurred within the six minutes of the scan time. Three sets of linear correlations will be generated. The first...

  4. Lightning strikes to tall objects: Currents inferred from far electromagnetic fields versus directly measured currents

    E-Print Network [OSTI]

    Florida, University of

    -field-to-current conversion factor for lightning strikes to flat ground based on the transmission line model and (b currents on tall towers are used for testing the validity of field-to-current conversion equations [e on current measurement location (e.g., near the top or bottom of the tower) and on whether initial or largest

  5. Environmental control of cloud-to-ground lightning polarity in severe storms

    E-Print Network [OSTI]

    Buffalo, Kurt Matthew

    2008-10-10T23:59:59.000Z

    polarity of severe storms by directly affecting their structural, dynamical, and microphysical properties, which in turn directly control cloud electrification and CG flash polarity. A more specific hypothesis, which has been supported by past............................................................................... 23 a. Thunderstorm electrification ................................................ 23 1) Charging mechanisms and typical charge structure ... 23 2) Cloud-to-ground lightning flash................................. 27 3...

  6. DNA Computing Complexity Analysis Using DNA/DNA Hybridization Kinetics

    E-Print Network [OSTI]

    DNA Computing Complexity Analysis Using DNA/DNA Hybridization Kinetics SooYong Shin 1 , Eun Jeong the complexity of DNA computing. The complexity of any computational algorithm is typically measured in terms of time and space. In DNA computing, the time complexity can be measured by the total reaction time

  7. DNA Computing Complexity Analysis Using DNA/DNA Hybridization Kinetics

    E-Print Network [OSTI]

    DNA Computing Complexity Analysis Using DNA/DNA Hybridization Kinetics Soo-Yong Shin1 , Eun Jeong of DNA computing. The complexity of any computational algorithm is typically measured in terms of time and space. In DNA computing, the time complexity can be measured by the total reaction time

  8. IEEE I&CPS Conf., May 7-11, San Antonio, Texas, pp. 147-152 Reliability of Lightning Resistant

    E-Print Network [OSTI]

    Tolbert, Leon M.

    IEEE I&CPS Conf., May 7-11, San Antonio, Texas, pp. 147-152 Reliability of Lightning Resistant essentially remained unchanged in the last 32 years which allows a meaningful comparison of reliability trends

  9. Analysis of the Texas A&M impact lightning detector to determine detection efficiency and site error corrections

    E-Print Network [OSTI]

    Leahy, Frank Byron

    1999-01-01T23:59:59.000Z

    The Texas A&M IMProved Accuracy from Combined Technology (IMPACT) lightning detector has been analyzed for 1997 and 1998-99 to determine detection characteristics. These characteristics include stroke detection efficiency as a function of range...

  10. Cloud-to-ground lightning characteristics of warm season Mesoscale Convection Systems in the Central United States: 1992-1993

    E-Print Network [OSTI]

    Hoeth, Brian Richard

    1998-01-01T23:59:59.000Z

    This study provides a detailed analysis of cloud-to-aphics. ground (CG) lightning flashes within individual Mesoscale Convective Systems (MCSs) that occurred in the Central United States during May-August of 1992 and 1993. Analysis of the CG...

  11. The evolution of total lightning and radar reflectivity characteristics of two mesoscale convective systems over Houston, Texas

    E-Print Network [OSTI]

    Hodapp, Charles Lee

    2009-05-15T23:59:59.000Z

    originated in the convective or transition regions. Both in-situ charging mechanisms created by the development of the mesoscale updraft and charge advection by the front-to-rear flow likely contribute to the increased electrification and lightning...

  12. (gene expression) DNA (DNA microarrays).

    E-Print Network [OSTI]

    Athens, University of

    Lymphoblastic Leukemia - ALL, 25 Acute Myeloid Leukemia - AML) 7129 [10]. DNA. 62 , 22 40 , 2000 [6]. 72 (47 Acute

  13. ##### SAT Engine ####### _ ############ DNA ###### _

    E-Print Network [OSTI]

    Hagiya, Masami

    ##### SAT Engine ####### _ ############ DNA ###### _ # # # #y # # #yy # # # #yy ###### DNA #################################### ############### ##################### 6 ## 10 ##### ### DNA ############### (Sakamoto et al., Science, Vol.288, pp.1223-122* *6

  14. New Revelation of Lightning Ball Observation and Proposal for a Nuclear Reactor Fusion Experiment

    E-Print Network [OSTI]

    Domokos Tar

    2009-10-12T23:59:59.000Z

    In this paper, the author brings further details regarding his Lightning Ball observation that were not mentioned in the first one (Ref.1-2). Additionally, he goes more into detail as the three forces that are necessary to allow the residual crescent form the hydrodynamic vortex ring to shrink into a sphere.Further topics are the similarities and analogies between the Lightning Ball formation's theory and the presently undertaken Tokamak-Stellarator-Spheromak fusion reactor experiments. A new theory and its experimental realisation are proposed as to make the shrinking of the hot plasma of reactors into a ball possible by means of the so called long range electromagnetic forces. In this way,the fusion ignition temperature could possibly atteined.

  15. New Revelation of Lightning Ball Observation and Proposal for a Nuclear Reactor Fusion Experiment

    E-Print Network [OSTI]

    Tar, Domokos

    2009-01-01T23:59:59.000Z

    In this paper, the author brings further details regarding his Lightning Ball observation that were not mentioned in the first one (Ref.1-2). Additionally, he goes more into detail as the three forces that are necessary to allow the residual crescent form the hydrodynamic vortex ring to shrink into a sphere.Further topics are the similarities and analogies between the Lightning Ball formation's theory and the presently undertaken Tokamak-Stellarator-Spheromak fusion reactor experiments. A new theory and its experimental realisation are proposed as to make the shrinking of the hot plasma of reactors into a ball possible by means of the so called long range electromagnetic forces. In this way,the fusion ignition temperature could possibly atteined.

  16. Ignition probabilities of wildland fuels based on simulated lightning discharges. Forest Service research paper

    SciTech Connect (OSTI)

    Latham, D.J.; Schlieter, J.A.

    1989-09-01T23:59:59.000Z

    Ignition of wildland fine fuels by lightning was simulated with an electric arc discharge in the laboratory. The results showed that fuel parameters such as depth, moisture content, bulk density, and mineral content can be combined with the duration of the simulated continuing current to give ignition probabilities. The fuel state parameters of importance and the ignition probabilities were determined using logistic regression. Graphs, tables, formulas, and a FORTRAN computer program are given for field use.

  17. A Ball Lightning Model as a Possible Explanation of Recently Reported Cavity Lights

    SciTech Connect (OSTI)

    Fryberger, David; /SLAC

    2009-08-04T23:59:59.000Z

    The salient features of cavity lights, in particular, mobile luminous objects (MLO's), as have been experimentally observed in superconducting accelerator cavities, are summarized. A model based upon standard electromagnetic interactions between a small particle and the 1.5 GHz cavity excitation field is described. This model can explain some features of these data, in particular, the existence of particle orbits without wall contact. While this result is an important success for the model, it is detailed why the model as it stands is incomplete. It is argued that no avenues for a suitable extension of the model through established physics appear evident, which motivates an investigation of a model based upon a more exotic object, ball lightning. As discussed, further motivation derives from the fact that there are significant similarities in many of the qualitative features of ball lightning and MLO's, even though they appear in quite different circumstances and differ in scale by orders of magnitude. The ball lightning model, which incorporates electromagnetic charges and currents, is based on a symmetrized set of Maxwell's equations in which the electromagnetic sources and fields are characterized by a process called dyality rotation. It is shown that a consistent mathematical description of dyality rotation as a physical process can be achieved by adding suitable (phenomenological) current terms to supplement the usual current terms in the symmetrized Maxwell's equations. These currents, which enable the conservation of electric and magnetic charge, are called vacuum currents. It is shown that the proposed ball lightning model offers a good qualitative explanation of the perplexing aspects of the MLO data. Avenues for further study are indicated.

  18. Measurement and modeling of transfer functions for lightning coupling into the Sago mine.

    SciTech Connect (OSTI)

    Morris, Marvin E.; Higgins, Matthew B.

    2007-04-01T23:59:59.000Z

    This report documents measurements and analytical modeling of electromagnetic transfer functions to quantify the ability of cloud-to-ground lightning strokes (including horizontal arc-channel components) to couple electromagnetic energy into the Sago mine located near Buckhannon, WV. Two coupling mechanisms were measured: direct and indirect drive. These transfer functions are then used to predict electric fields within the mine and induced voltages on conductors that were left abandoned in the sealed area of the Sago mine.

  19. Adleman[1] 1994 DNA Hamiltonian Path Problem , DNA

    E-Print Network [OSTI]

    1. Adleman[1] 1994 DNA Hamiltonian Path Problem , DNA DNA [2]. DNA DNA , . , , 2 , DNA 4 . DNA 4 A(Adenine), C(Cytosine), G(Guanine), T(Thymine) 2 4 . , . 1 mole 6x10 23 DNA DNA . , . DNA NP-complete [1, 2], [2

  20. DNA Topology: Fundamentals

    E-Print Network [OSTI]

    Mirkin, Sergei

    DNA Topology: Fundamentals Sergei M Mirkin, University of Illinois at Chicago, Illinois, USA Topological characteristics of DNA and specifically DNA supercoiling influence all major DNA transactions in living cells. DNA supercoiling induces the formation of unusual secondary structure by specific DNA

  1. Problem 65 in Section 4.1 (Page 274) Constructing a pipeline Supertankers off-load oil at a docking facility 4 mi offshore. The nearest refinery

    E-Print Network [OSTI]

    Schilling, Anne

    facility 4 mi offshore. The nearest refinery is 9 mi east of the shore point nearest the docking facility. A pipeline must be constructed connecting the docking facility with the refinery. The pipeline costs $300.42 miles away from the refinery, or equivalently 3.58 miles away from Point A (as the back of the book has

  2. DNA polymerase with modified processivity

    DOE Patents [OSTI]

    Bedford, Ella (Brookline, MA); Tabor, Stanley (Cambridge, MA); Richardson, Charles C. (Chestnut Hill, MA)

    1999-01-01T23:59:59.000Z

    Chimeric DNA polymerase having a DNA polymerase domain and processivity factor binding domain not naturally associated with DNA polymerase domain.

  3. 498 IEEE JOURNAL OF OCEANIC ENGINEERING, VOL. 26, NO. 4, OCTOBER 2001 Docking for an Autonomous Ocean Sampling

    E-Print Network [OSTI]

    Singh, Hanumant

    498 IEEE JOURNAL OF OCEANIC ENGINEERING, VOL. 26, NO. 4, OCTOBER 2001 Docking for an Autonomous of Applied Ocean Physics and Engineering, Woods Hole Oceano- graphic Institution, Woods Hole, MA 02543 USA (e is with the Department of Ocean Engineering, Massachusetts Insti- tute of Technology, Cambridge, MA 02139 USA (e

  4. Thermodynamic Perspective on the Dock-Lock Growth Mechanism of Amyloid Fibrils Edward P. O'Brien,,

    E-Print Network [OSTI]

    Thirumalai, Devarajan

    for Physical Science and Technology, Department of Chemistry and Biochemistry, UniVersity of Maryland, College, Japan, and Department of Chemistry, Boston UniVersity, Boston, Massachusetts 02215 ReceiVed: May 28 (docking) followed by a slower conformational rearrangement leading to the incorporation onto

  5. Relationships between tropical cyclone intensity and satellite based indicators of inner core convection: 85 GHz ice scattering signature and lightning

    E-Print Network [OSTI]

    Cecil, Daniel Joseph

    1997-01-01T23:59:59.000Z

    -1' radius minimum PCT and lightning in Hurricane Felix. . . . . 48 22 Hurricane Luis best track, 12 UTC 27 August - 0 UTC 11 September 1995. . . ?50 23 Luis 85 GHz PCT iield at 0748 V TC 29 August 1995. . . . . . 57 24 Luis OTD lightning observations... at 2348 UTC 28 August 1995. . . . . 25 Luis 85 GHz PCT field at 0905 UTC 30 August 1995. . . . 54 26 Luis 85 GHz PCT field at 2017 UTC 31 August 1995. . . . . . . . 27 Luis 85 GHz PCT field at 0841 UTC 2 September 1995. . . . 28 Luis 85 GHz PCT field...

  6. Voltages across assembly joints due to direct-strike lightning currents

    SciTech Connect (OSTI)

    Dinallo, M.S. [Quatro Corp., Albuquerque, NM (United States); Fisher, R.J. [Sandia National Labs., Albuquerque, NM (United States)

    1994-08-01T23:59:59.000Z

    An extensive set of direct-strike lightning tests has been carried out on a set of facsimile assembly joints of the kinds employed in the design of nuclear weapon cases. Taken as a whole, the test hardware included all the conceptual design elements that are embodied, either singly or in combination, in any specific assembly joint incorporated into any stockpiled weapon. During the present testing, the effects of all key design parameters on the voltages developed across the interior of the joints were investigated under a range of lightning stroke current parameter values. Design parameter variations included the types and number of joint fasteners, mechanical preload, surface finish tolerance and coatings, and the material from which the joint assembly was fabricated. Variations of the simulated lightning stroke current included amplitude (30-, 100-, and 200-kA peak), rise time, and decay time. The maximum voltage observed on any of the test joints that incorporated proper metal-to-metal surface contact was 65 V. Typical response values were more on the order of 20 V. In order to assess the effect of the presence of a dielectric coating (either intentional or as a result of corrosion) between the mating surfaces of a joint, a special configuration was tested in which the mating parts of the test assembly were coated with a 1-mil-thick dielectric anodizing layer. First strokes to these test assemblies resulted in very narrow voltage spikes of amplitudes up to 900 V. The durations of these spikes were less than 0.1 {mu}s. However, beyond these initial spikes, the voltages typically had amplitudes of up to 400 V for durations of 3 to 5 {mu}s.

  7. A unified radiative magnetohydrodynamics code for lightning-like discharge simulations

    SciTech Connect (OSTI)

    Chen, Qiang, E-mail: cq0405@126.com; Chen, Bin, E-mail: emcchen@163.com; Xiong, Run; Cai, Zhaoyang [National Key Laboratory of Electromagnetic Environment and Electro-optical Engineering, PLA University of Science and Technology, Nanjing 210007 (China)] [National Key Laboratory of Electromagnetic Environment and Electro-optical Engineering, PLA University of Science and Technology, Nanjing 210007 (China); Chen, P. F., E-mail: chenpf@nju.edu.cn [School of Astronomy and Space Science, Nanjing University, Nanjing 210093 (China)

    2014-03-15T23:59:59.000Z

    A two-dimensional Eulerian finite difference code is developed for solving the non-ideal magnetohydrodynamic (MHD) equations including the effects of self-consistent magnetic field, thermal conduction, resistivity, gravity, and radiation transfer, which when combined with specified pulse current models and plasma equations of state, can be used as a unified lightning return stroke solver. The differential equations are written in the covariant form in the cylindrical geometry and kept in the conservative form which enables some high-accuracy shock capturing schemes to be equipped in the lightning channel configuration naturally. In this code, the 5-order weighted essentially non-oscillatory scheme combined with Lax-Friedrichs flux splitting method is introduced for computing the convection terms of the MHD equations. The 3-order total variation diminishing Runge-Kutta integral operator is also equipped to keep the time-space accuracy of consistency. The numerical algorithms for non-ideal terms, e.g., artificial viscosity, resistivity, and thermal conduction, are introduced in the code via operator splitting method. This code assumes the radiation is in local thermodynamic equilibrium with plasma components and the flux limited diffusion algorithm with grey opacities is implemented for computing the radiation transfer. The transport coefficients and equation of state in this code are obtained from detailed particle population distribution calculation, which makes the numerical model is self-consistent. This code is systematically validated via the Sedov blast solutions and then used for lightning return stroke simulations with the peak current being 20?kA, 30?kA, and 40?kA, respectively. The results show that this numerical model consistent with observations and previous numerical results. The population distribution evolution and energy conservation problems are also discussed.

  8. Advance the DNA computing

    E-Print Network [OSTI]

    Qiu, Zhiquan Frank

    2004-09-30T23:59:59.000Z

    DNA computer. The existing models from which a few DNA computing algorithms have been developed are not sufficiently powerful and robust, however, to attract potential users. This thesis has described research performed to build a new DNA computing...

  9. The convective structures associated with cloud-to-ground lightning in TOGA COARE Mesoscale Convective Systems

    E-Print Network [OSTI]

    Restivo, Michael Edward

    1995-01-01T23:59:59.000Z

    suggested that the threshold of about 40 dBZ at the -10 C level for rapid cloud electrification found in New Mexico by Dye et al. (1989) could be valid for tropical convection as well. Orville and Henderson (1986), and Goodman and Christian (1993), have... along with small ice and supercooled liquid water for cloud electrification and lightning to occur. Since most oceanic VPRR drop off rapidly above the freezing level compared to continental VPRR, this would provide evidence that the updraft velocities...

  10. Synthesis of DNA

    DOE Patents [OSTI]

    Mariella, Jr., Raymond P. (Danville, CA)

    2008-11-18T23:59:59.000Z

    A method of synthesizing a desired double-stranded DNA of a predetermined length and of a predetermined sequence. Preselected sequence segments that will complete the desired double-stranded DNA are determined. Preselected segment sequences of DNA that will be used to complete the desired double-stranded DNA are provided. The preselected segment sequences of DNA are assembled to produce the desired double-stranded DNA.

  11. DNA encoding a DNA repair protein

    DOE Patents [OSTI]

    Petrini, John H.; Morgan, William Francis; Maser, Richard Scott; Carney, James Patrick

    2006-08-15T23:59:59.000Z

    An isolated and purified DNA molecule encoding a DNA repair protein, p95, is provided, as is isolated and purified p95. Also provided are methods of detecting p95 and DNA encoding p95. The invention further provides p95 knock-out mice.

  12. Measured voltages and currents internal to closed metal cylinders due to diffusion of simulated lightning currents

    SciTech Connect (OSTI)

    Schnetzer, G.H.; Fisher, R.J.

    1994-08-01T23:59:59.000Z

    One mechanism for the penetration of lightning energy into the interior of a weapon is by current diffusion through the exterior metal case. Tests were conducted in which simulated lightning currents were driven over the exteriors of similar aluminum and ferrous steel cylinders of 0.125-in wall thickness. Under conditions in which the test currents were driven asymmetrically over the exteriors of the cylinders, voltages were measured between various test points in the interior as functions of the amplitude and duration of the applied current. The maximum recorded open-circuit voltage, which occurred in the steel cylinder, was 1.7 V. On separate shots, currents flowing on a low impedance shorting conductor between the same set of test points were also measured, yielding a maximum current of 630 A, again occurring across the interior of the steel cylinder. Under symmetrical exterior drive current conditions, a maximum end-to-end internal voltage of 4.1 V was obtained, also in the steel cylinder, with a corresponding current of 480 A measured on a coaxial conductor connected between the two end plates of the cylinder. Data were acquired over a range of input current amplitudes between about 40 and 100 kA. These data provide the experimental basis for validating models that can subsequently be applied to real weapons and other objects of interest.

  13. Physical mechanism and numerical simulation of the inception of the lightning upward leader

    SciTech Connect (OSTI)

    Li Qingmin [Beijing Key Lab of High Voltage and EMC, School of Electric and Electronic Engineering, North China Electric Power University, Beijing 102206 (China) and State Key Lab of Alternate Electrical Power System with Renewable Energy Sources, North China Electric Power University, Beijing 102206 (China); Lu Xinchang; Shi Wei; Zhang Li; Zou Liang; Lou Jie [Shandong Provincial Key Lab of UHV Technology and Gas Discharge, School of Electrical Engineering, Shandong University, Jinan 250061 (China)

    2012-12-15T23:59:59.000Z

    The upward leader is a key physical process of the leader progression model of lightning shielding. The inception mechanism and criterion of the upward leader need further understanding and clarification. Based on leader discharge theory, this paper proposes the critical electric field intensity of the stable upward leader (CEFISUL) and characterizes it by the valve electric field intensity on the conductor surface, E{sub L}, which is the basis of a new inception criterion for the upward leader. Through numerical simulation under various physical conditions, we verified that E{sub L} is mainly related to the conductor radius, and data fitting yields the mathematical expression of E{sub L}. We further establish a computational model for lightning shielding performance of the transmission lines based on the proposed CEFISUL criterion, which reproduces the shielding failure rate of typical UHV transmission lines. The model-based calculation results agree well with the statistical data from on-site operations, which show the effectiveness and validity of the CEFISUL criterion.

  14. Report on an Explosion and Fire involving the Motor Vessel Atlantic Duchess at Queen's Dock, Swansea, Glamorganshire on 2nd February, 1951

    E-Print Network [OSTI]

    Watts, H. E.

    1951-04-02T23:59:59.000Z

    REPORT TO THE RIGHT HONOURABLE THE SECRETARY OF STATE FOR THE HOME DEPARTMENT ON THE CIRCUMSTANCES ATTENDING AN EXPLOSION AND FIRE WHICH OCCURRED ON 2ND FEBRUARY. 1951, AT QUEEN'S DOCK SWANSEA, IN THE COUNTY OF ...

  15. A classification of Texas thunderstorms according to their cloud-to-ground lightning characteristics during spring 1993

    E-Print Network [OSTI]

    McMillan, Stephen Randall

    1995-01-01T23:59:59.000Z

    were positive; the positive flashes exhibited a mean multiplicity of 1.43 and a median peak current of 36.6 kA. Relating the data to storm type revealed that frontal storm lightning was 2-3 times more positive than observed in airmass storms. Negative...

  16. P1.15 A GIS-Based Approach to Lightning Studies for West Texas and New Mexico

    E-Print Network [OSTI]

    Fuelberg, Henry

    P1.15 A GIS-Based Approach to Lightning Studies for West Texas and New Mexico Geoffrey Wagner here. Our study domain is New Mexico and West Texas, as illustrated by the red box in Fig. 1 eastern part of the domain (West Texas), which contains caprock features near Amarillo. A major landmark

  17. PUBLS. INST. GEOPHYS. POL. ACAD. SC., D-73 (412), 2009 Dynamics of Lightning Channel Corona Sheath Predicted

    E-Print Network [OSTI]

    Florida, University of

    consists of a thin core surrounded by a radially formed corona sheath. The corona sheath expands outward, the leader charge stored in the corona sheath collapses into the channel core and is transferred to groundPUBLS. INST. GEOPHYS. POL. ACAD. SC., D-73 (412), 2009 Dynamics of Lightning Channel Corona Sheath

  18. IEEE TRANSACTIONS ON ELECTROMAGNETIC COMPATIBILITY, VOL. 51, NO. 3, AUGUST 2009 471 New Insights Into Lightning Return-Stroke Models

    E-Print Network [OSTI]

    Florida, University of

    core are examined based on LCS and DCS models. Index Terms--Continuity equation, corona sheath. Dynamics of lightning-channel corona sheath sur- rounding the thin channel core are examined based continuity equation, in terms of two cur- rent components, propagation current and corona current. For lumped

  19. Charge transfer and in-cloud structure of large-charge-moment positive lightning strokes in a mesoscale convective system

    E-Print Network [OSTI]

    Cummer, Steven A.

    in a mesoscale convective system Gaopeng Lu,1 Steven A. Cummer,1 Jingbo Li,1 Feng Han,1 Richard J. Blakeslee,2 positive cloud-to-ground (+CG) strokes in a mesoscale convective system. Although no high altitude images of large-charge-moment positive lightning strokes in a mesoscale convective system, Geophys. Res. Lett., 36

  20. A monthly climatological study of cloud-to-ground lightning characteristics throughout the contiguous United States: 1989-1994

    E-Print Network [OSTI]

    Silver, Alan Corey

    1995-01-01T23:59:59.000Z

    higher values, appeared from Minnesota southwestward into Colorado from April through August. Mean monthly percentage positive lightning was highest in December at 16.0% and lowest in August at 3.0%. The mean monthly negative multiplicity ranged from 2...

  1. Electric and magnetic fields and field derivatives from lightning stepped leaders and first return strokes measured at distances

    E-Print Network [OSTI]

    Florida, University of

    Electric and magnetic fields and field derivatives from lightning stepped leaders and first return; published 5 September 2008. [1] Using electric and magnetic field and field derivative sensors arrayed over-peak width of the stepped-leader/return-stroke electric field waveform; the stepped-leader electric field

  2. IEEE TRANSACTIONS ON ELECTROMAGNETIC COMPATIBILITY, VOL. 50, NO. 3, AUGUST 2008 651 Analysis of Lightning-Induced Voltages on

    E-Print Network [OSTI]

    Florida, University of

    over lossy ground produced by lightning strikes either to flat ground or to a tall tower are calculated by return-stroke speed, and essentially inde- pendent of return-stroke model [transmission-line (TL), modified transmission line with linear current decay with height (MTLL), or modified transmission line

  3. Quantitative DNA fiber mapping

    DOE Patents [OSTI]

    Gray, Joe W. (San Francisco, CA); Weier, Heinz-Ulrich G. (Oakland, CA)

    1998-01-01T23:59:59.000Z

    The present invention relates generally to the DNA mapping and sequencing technologies. In particular, the present invention provides enhanced methods and compositions for the physical mapping and positional cloning of genomic DNA. The present invention also provides a useful analytical technique to directly map cloned DNA sequences onto individual stretched DNA molecules.

  4. IMPEE PhD Opportunity Project title: LightFoot PhD A PhD Investigation Lightning Protection of HV Overhead Lines with Non-Ideal

    E-Print Network [OSTI]

    Greenaway, Alan

    IMPEE PhD Opportunity Project title: LightFoot PhD A PhD Investigation Lightning Protection of HV.swingler@hw.ac.uk Abstract LightFoot PhD A PhD Investigation Lightning Protection of HV Overhead Lines with Non-Ideal Tower Footing Resistance funded by SSE. The LightFoot PhD project takes a fresh approach at adding

  5. Natural DNA sequencing by synthesis

    E-Print Network [OSTI]

    Roller, Eric E.

    2011-01-01T23:59:59.000Z

    labeled nucleotides by DNA polymerases. Biotechniques, 2005.S.A. , et al. , Multiplexed DNA sequencing-by-synthesis.Assembly of High-Density DNA Arrays for Genomic Analyses.

  6. Comments to support the Dipole Dynamical Model (DDM) of Ball Lightning (BL)

    E-Print Network [OSTI]

    V. N. Soshnikov

    2015-02-01T23:59:59.000Z

    I present estimates to justify previously proposed by me heuristic Dipole Dynamical Model (DDM) of Ball Lightning (BL). The movement and energy supplying to the dipole BL are due to the atmospheric electric field. Crucial for the detailed analysis of BL is using the new relation of balance of the force of atmospheric electric field (per unit mass of electron cloud) and dipole forces electrons-ions within BL dipole (per unit mass of BL) as the first necessary condition for the existance of BL as an integer. This model is unique because, unlike existing static models, fundamental condition for the existence of Ball Lightning is its forward motion. The virial theorem limiting BL power does not apply to BL which is not closed system like the Sun or Galaxy systems and is strongly dependent part of the infinitely extended in time and space large system. Stability of BL is due to two free parameters with the fundamental role of thermodynamic non-equilibrium, ionization, recombination and translational movement with energy loss by radiation and also excess volumetric positive charge. Polarization degree of BL plasma is characterized by polarizability factor {\\gamma}. An example is presented of calculating the stability of option BL. There is also a possible connection of stability BL with statistical distributions of the atmospheric electric field in time and space. Destruction of BL can also occur due to arising kinematical instability at its accelerating (or decelerating) movement. Maximal energy density in BL DDM does not exceed the value Espec<(10(8) - 10(9)) J/m(3). Resulting indefinitely long BL lifetime is also discussed.

  7. Comments to support the Dipole Dynamical Model (DDM) of Ball Lightning (BL)

    E-Print Network [OSTI]

    V. N. Soshnikov

    2015-06-22T23:59:59.000Z

    I present estimates to justify previously proposed by me heuristic Dipole Dynamical Model (DDM) of Ball Lightning (BL). The movement and energy supplying to the dipole BL are due to the atmospheric electric field. Crucial for the detailed analysis of BL is using the new relation of balance of the force of atmospheric electric field (per unit mass of electron cloud) and dipole forces electrons-ions within BL dipole (per unit mass of BL) as the first necessary condition for the existance of BL as an integer. This model is unique because, unlike existing static models, fundamental condition for the existence of Ball Lightning is its forward motion. The virial theorem limiting BL power does not apply to BL which is not closed system like the Sun or Galaxy systems and is strongly dependent part of the infinitely extended in time and space large system. Stability of BL is due to two free parameters with the fundamental role of thermodynamic non-equilibrium, ionization, recombination and translational movement with energy loss by radiation and also excess volumetric positive charge. Stability of BL is not related to the presence of any external shells. Polarization degree of BL plasma is characterized by polarizability factor {\\gamma}. An example is presented of calculating the stability of option BL. There is also a possible connection of stability BL with statistical distributions of the atmospheric electric field in time and space. Destruction of BL can also occur due to arising kinematical instability at its accelerating (or decelerating) movement. Maximal energy density in BL DDM does not exceed the value Espec<(10(8) - 10(9)) J/m(3). Resulting indefinitely long BL lifetime is also discussed.

  8. DNA conjugation andDNA conjugation and reversibility onreversibility on

    E-Print Network [OSTI]

    Rubloff, Gary W.

    DNA conjugation andDNA conjugation and reversibility onreversibility on chitosan surfaceschitosan surfaceschitosan surfaceschitosan surfaces Rubloff Research Group Accomplishments #12;DNA conjugation and reversibility onDNA conjugation and reversibility on chitosan surfaceschitosan surfaces Accomplishment Single

  9. Meta-DNA: synthetic biology via DNA nanostructures and

    E-Print Network [OSTI]

    Reif, John H.

    Meta-DNA: synthetic biology via DNA nanostructures and hybridization reactions Harish Chandran1 of DNA manipulations achieved by protein enzymes be simulated via simple DNA hybridization chemistry? In this work, we develop a biochemical system which we call meta-DNA (abbreviated as mDNA), based on strands

  10. Quantitive DNA Fiber Mapping

    E-Print Network [OSTI]

    Lu, Chun-Mei

    2009-01-01T23:59:59.000Z

    of California. Lu et al. : DNA Fiber Mapping page - 35 Lu etal. : DNA Fiber Mapping page - 36 a b c d e f g OV P1 cloneSp6 end T7 end Lu et al. : DNA Fiber Mapping page - 37 a b c

  11. Combined 3D-QSAR, molecular docking and molecular dynamics study on thyroid hormone activity of hydroxylated polybrominated diphenyl ethers to thyroid receptors ?

    SciTech Connect (OSTI)

    Li, Xiaolin [State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210046 (China)] [State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210046 (China); Ye, Li [Suzhou NeuPharma Co.,Ltd, Suzhou 215123 (China)] [Suzhou NeuPharma Co.,Ltd, Suzhou 215123 (China); Wang, Xiaoxiang [State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210046 (China)] [State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210046 (China); Wang, Xinzhou [Suzhou NeuPharma Co.,Ltd, Suzhou 215123 (China)] [Suzhou NeuPharma Co.,Ltd, Suzhou 215123 (China); Liu, Hongling [State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210046 (China)] [State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210046 (China); Zhu, Yongliang [Suzhou NeuPharma Co.,Ltd, Suzhou 215123 (China)] [Suzhou NeuPharma Co.,Ltd, Suzhou 215123 (China); Yu, Hongxia, E-mail: hongxiayu01@yahoo.com.cn [State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210046 (China)] [State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210046 (China)

    2012-12-15T23:59:59.000Z

    Several recent reports suggested that hydroxylated polybrominated diphenyl ethers (HO-PBDEs) may disturb thyroid hormone homeostasis. To illuminate the structural features for thyroid hormone activity of HO-PBDEs and the binding mode between HO-PBDEs and thyroid hormone receptor (TR), the hormone activity of a series of HO-PBDEs to thyroid receptors ? was studied based on the combination of 3D-QSAR, molecular docking, and molecular dynamics (MD) methods. The ligand- and receptor-based 3D-QSAR models were obtained using Comparative Molecular Similarity Index Analysis (CoMSIA) method. The optimum CoMSIA model with region focusing yielded satisfactory statistical results: leave-one-out cross-validation correlation coefficient (q{sup 2}) was 0.571 and non-cross-validation correlation coefficient (r{sup 2}) was 0.951. Furthermore, the results of internal validation such as bootstrapping, leave-many-out cross-validation, and progressive scrambling as well as external validation indicated the rationality and good predictive ability of the best model. In addition, molecular docking elucidated the conformations of compounds and key amino acid residues at the docking pocket, MD simulation further determined the binding process and validated the rationality of docking results. -- Highlights: ? The thyroid hormone activities of HO-PBDEs were studied by 3D-QSAR. ? The binding modes between HO-PBDEs and TR? were explored. ? 3D-QSAR, molecular docking, and molecular dynamics (MD) methods were performed.

  12. DNA Sequencing apparatus

    DOE Patents [OSTI]

    Tabor, Stanley (Cambridge, MA); Richardson, Charles C. (Chestnut Hill, MA)

    1992-01-01T23:59:59.000Z

    An automated DNA sequencing apparatus having a reactor for providing at least two series of DNA products formed from a single primer and a DNA strand, each DNA product of a series differing in molecular weight and having a chain terminating agent at one end; separating means for separating the DNA products to form a series bands, the intensity of substantially all nearby bands in a different series being different, band reading means for determining the position an This invention was made with government support including a grant from the U.S. Public Health Service, contract number AI-06045. The U.S. government has certain rights in the invention.

  13. DNA: structure, dense phases, charges, interactions

    E-Print Network [OSTI]

    Potsdam, Universitt

    DNA: structure, dense phases, charges, interactions #12;Outline 1. DNA: structure, charges, dense phases 2. Counterion and DNA condensation 3. ES DNA-DNA interactions 4. DNA toroidal structures 5. Interactions of real DNA helices 6. DNA-DNA ES recognition 7. DNA melting in aggregates 8. Azimuthal

  14. Detection and quantitation of single nucleotide polymorphisms, DNA sequence variations, DNA mutations, DNA damage and DNA mismatches

    SciTech Connect (OSTI)

    McCutchen-Maloney, Sandra L. (Pleasanton, CA)

    2002-01-01T23:59:59.000Z

    DNA mutation binding proteins alone and as chimeric proteins with nucleases are used with solid supports to detect DNA sequence variations, DNA mutations and single nucleotide polymorphisms. The solid supports may be flow cytometry beads, DNA chips, glass slides or DNA dips sticks. DNA molecules are coupled to solid supports to form DNA-support complexes. Labeled DNA is used with unlabeled DNA mutation binding proteins such at TthMutS to detect DNA sequence variations, DNA mutations and single nucleotide length polymorphisms by binding which gives an increase in signal. Unlabeled DNA is utilized with labeled chimeras to detect DNA sequence variations, DNA mutations and single nucleotide length polymorphisms by nuclease activity of the chimera which gives a decrease in signal.

  15. (2) DNA O(n^5) Quorum-Sensing Lux

    E-Print Network [OSTI]

    Hagiya, Masami

    - 1 - ( ) ( ) DNA RNA DNA RNA DNA DNA 2 DNA #12;- 2 - 17 6 (1) (2) DNA O(n^5) (3) Quorum-Sensing Lux (4) (5) LMNtal ambient LMNtal (1) (2) DNA (3) DNA (4) DNA (5) DNA (1) DNA ANP-96 (Precision System Science ) (2) RTRACS DNA RTRACS (3) in vivo in vivo (4) DNA trans cis 1/10 (5) DNA-PNA DNA DNA DNA DNA DNA

  16. Electrochemical DNA Hybridization Detection Using DNA Dohyoung Kwon,a

    E-Print Network [OSTI]

    Kwak, Juhyoun

    Full Paper Electrochemical DNA Hybridization Detection Using DNA Cleavage Dohyoung Kwon,a Kyuwon method for detection of DNA hybridization using enzymatic cleavage. The strategy is based on that S1 nuclease is able to specifically cleave only single strand DNA, but not double strand DNA. The capture

  17. Measured responses of internal enclosures and cables due to burnthrough penetration of weapon cases by lightning

    SciTech Connect (OSTI)

    Schnetzer, G.H.; Fisher, R.J. [Sandia National Labs., Albuquerque, NM (United States); Dinallo, M.A. [Quatro Corp., Albuquerque, NM (United States)

    1994-08-01T23:59:59.000Z

    The electrical effects of lightning penetration of the outer case of a weapon on internal structures, such as a firing set housing, and on samples of a flat, flexline detonator cable have been investigated experimentally. Maximum open-circuit voltages measured on either simulated structures (126 V) or the cable (46 V) located directly behind the point of penetration were well below any level that is foreseen to create a threat to nuclear safety. On the other hand, it was found that once full burnthrough of the barrier occurred, significant fractions of the incident continuing currents coupled to both the simulated internal structure (up to 300 A) or to the cable sample (69 A) when each was electrically connected internally to case ground. No occurrence was observed of the injection of large amplitude currents from return strokes occurring after barrier penetration. Under circumstances in which small volumes of trapped gases exist behind penetration sites, rapid heating of the gas by return strokes occurring after burnthrough has been shown to produced large mechanical impulses to the adjacent surfaces.

  18. DNA ELECTROPHORESIS AT SURFACES

    SciTech Connect (OSTI)

    RAFAILOVICH, MIRIAM; SOKOLOV, JONATHAN; GERSAPPE, DILIP

    2003-09-01T23:59:59.000Z

    During this year we performed two major projects: I. We developed a detailed theoretical model which complements our experiments on surface DNA electrophoresis. We found that it was possible to enhance the separation of DNA chains by imposing a chemical nanoscale pattern on the surface. This approach utilized the surface interaction effect of the DNA chains with the substrate and is a refinement to our previous method in which DNA chains were separated on homogeneous flat surfaces. By introducing the nano-patterns on the surface, the conformational changes of DNA chains of different lengths can be amplified, which results in the different friction strengths with the substrate surface. Our results also show that, when compared to the DNA electrophoresis performed on homogeneous flat surfaces, nanopatterned surfaces offer a larger window in choosing different surface interactions to achieve separation. II. In collaboration with a large international manufacturer of skin care products we also embarked on a project involving photo toxicity of titanium dioxide nanoparticles, which are a key ingredient in sunscreen and cosmetic lotions. The results clearly implicated the nanoparticles in catalyzing damage to chromosomal DNA. We then used this knowledge to develop a polymer/anti-oxidant coating which prevented the photocatalytic reaction on DNA while still retaining the UV absorptive properties of the nanoparticles. The standard gel electrophoresis was not sufficient in determining the extent of the DNA damage. The conclusions of this study were based predominantly on analysis obtained with the surface electrophoresis method.

  19. Multiprotein DNA looping

    E-Print Network [OSTI]

    Jose M. G. Vilar; Leonor Saiz

    2006-06-19T23:59:59.000Z

    DNA looping plays a fundamental role in a wide variety of biological processes, providing the backbone for long range interactions on DNA. Here we develop the first model for DNA looping by an arbitrarily large number of proteins and solve it analytically in the case of identical binding. We uncover a switch-like transition between looped and unlooped phases and identify the key parameters that control this transition. Our results establish the basis for the quantitative understanding of fundamental cellular processes like DNA recombination, gene silencing, and telomere maintenance.

  20. Annual Report: 2011-2012 Storm Season Sampling, Non-Dry Dock Stormwater Monitoring for Puget Sound Naval Shipyard, Bremerton, WA

    SciTech Connect (OSTI)

    Brandenberger, Jill M.; Metallo, David; Rupert, Brian; Johnston, Robert K.; Gebhart, Christine

    2013-07-03T23:59:59.000Z

    Annual PSNS non-dry dock storm water monitoring results for 2011-2012 storm season. Included are a brief description of the sampling procedures, storm event information, laboratory methods and data collection, a results and discussion section, and the conclusions and recommendations.

  1. Visualizing DNA What is it?

    E-Print Network [OSTI]

    Rose, Michael R.

    Visualizing DNA #12;What is it? Gel electrophoresis is one of the techniques scientists use to look at the DNA they have. This technique separates DNA by size. #12;How does it work? First a gel is prepared. Gels

  2. Chimeric proteins for detection and quantitation of DNA mutations, DNA sequence variations, DNA damage and DNA mismatches

    DOE Patents [OSTI]

    McCutchen-Maloney, Sandra L. (Pleasanton, CA)

    2002-01-01T23:59:59.000Z

    Chimeric proteins having both DNA mutation binding activity and nuclease activity are synthesized by recombinant technology. The proteins are of the general formula A-L-B and B-L-A where A is a peptide having DNA mutation binding activity, L is a linker and B is a peptide having nuclease activity. The chimeric proteins are useful for detection and identification of DNA sequence variations including DNA mutations (including DNA damage and mismatches) by binding to the DNA mutation and cutting the DNA once the DNA mutation is detected.

  3. A Shared Docking Motif in TRF1 and TRF2 Used for Differential Recruitment of Telomeric Proteins

    SciTech Connect (OSTI)

    Chen, Yong; Yang, Yuting; van Overbeek, Megan; Donigian, Jill R.; Baciu, Paul; de Lange, Titia; Lei, Ming (Michigan-Med); (Rockefeller)

    2008-05-01T23:59:59.000Z

    Mammalian telomeres are protected by a six-protein complex: shelterin. Shelterin contains two closely related proteins (TRF1 and TRF2), which recruit various proteins to telomeres. We dissect the interactions of TRF1 and TRF2 with their shared binding partner (TIN2) and other shelterin accessory factors. TRF1 recognizes TIN2 using a conserved molecular surface in its TRF homology (TRFH) domain. However, this same surface does not act as a TIN2 binding site in TRF2, and TIN2 binding to TRF2 is mediated by a region outside the TRFH domain. Instead, the TRFH docking site of TRF2 binds a shelterin accessory factor (Apollo), which does not interact with the TRFH domain of TRF1. Conversely, the TRFH domain of TRF1, but not of TRF2, interacts with another shelterin-associated factor: PinX1.

  4. DNA-PK assay

    DOE Patents [OSTI]

    Anderson, Carl W.; Connelly, Margery A.

    2004-10-12T23:59:59.000Z

    The present invention provides a method for detecting DNA-activated protein kinase (DNA-PK) activity in a biological sample. The method includes contacting a biological sample with a detectably-labeled phosphate donor and a synthetic peptide substrate defined by the following features to provide specific recognition and phosphorylation by DNA-PK: (1) a phosphate-accepting amino acid pair which may include serine-glutamine (Ser-Gln) (SQ), threonine-glutamine (Thr-Gln) (TQ), glutamine-serine (Gln-Ser) (QS), or glutamine-threonine (Gln-Thr) (QT); (2) enhancer amino acids which may include glutamic acid or glutamine immediately adjacent at the amino- or carboxyl- side of the amino acid pair and forming an amino acid pair-enhancer unit; (3) a first spacer sequence at the amino terminus of the amino acid pair-enhancer unit; (4) a second spacer sequence at the carboxyl terminus of the amino acid pair-enhancer unit, which spacer sequences may include any combination of amino acids that does not provide a phosphorylation site consensus sequence motif; and, (5) a tag moiety, which may be an amino acid sequence or another chemical entity that permits separating the synthetic peptide from the phosphate donor. A compostion and a kit for the detection of DNA-PK activity are also provided. Methods for detecting DNA, protein phosphatases and substances that alter the activity of DNA-PK are also provided. The present invention also provides a method of monitoring protein kinase and DNA-PK activity in living cells. -A composition and a kit for monitoring protein kinase activity in vitro and a composition and a kit for monitoring DNA-PK activities in living cells are also provided. A method for identifying agents that alter protein kinase activity in vitro and a method for identifying agents that alter DNA-PK activity in living cells are also provided.

  5. DNA chips --Integrated Chemical Circuits for DNADiagnosis and DNA computers

    E-Print Network [OSTI]

    Hagiya, Masami

    DNA chips -- Integrated Chemical Circuits for DNADiagnosis and DNA computers Akira Suyama, Associate Professor Institute of Physics, Graduate School of Arts and Sciences, The University of Tokyo DNA chips are si l i con or glassbased smal l surfaces on which many DNA ol i gonuc l eotides are i

  6. Focus: DNA probes

    SciTech Connect (OSTI)

    Not Available

    1986-11-01T23:59:59.000Z

    Progress in the development of DNA probes for the identification and quantitation of specific genetic sequences in biological samples is reviewed. Current research efforts in the development of DNA probes for the diagnosis of a wide variety of bacterial, viral, and other infectious diseases, such as herpes simplex and cytomegalovirus, and inherited genetic diseases such as cystic fibrosis and sickle cell anemia are discussed. Progress in development of DNA probe assays for cancer diagnosis, detection of Salmonella food poisoning, tissue typing (detection of histocompatibility antigens), mutagen screening, and animal diseases, among other applications is included.

  7. Department of Computer Science University of North Carolina at Chapel Hill March 2005 Electric arcs and lightning effects are ubiquitous in science

    E-Print Network [OSTI]

    Whitton, Mary C.

    Department of Computer Science University of North Carolina at Chapel Hill March 2005 Background Electric arcs and lightning effects are ubiquitous in science fiction and fantasy films. Fromthegenesisof discharge have a long history as a dramatic tool in the visual effects industry. Despite the popularity

  8. -DNA 1217 BK21-IT,

    E-Print Network [OSTI]

    - DNA 1217 BK21-IT, (MEC) (NRL) . . : : : : syshin@bi.snu.ac.kr ihlee@bi.snu.ac.kr btzhang@bi.snu.ac.kr 2004 9 16 2005 10 14 - DNA (DNA Sequence Design using -Multiobjective Evolutionary Algorithm) (Soo-Yong Shin) (In-Hee Lee) (Byoung-Tak Zhang) DNA

  9. Shear Unzipping of DNA

    E-Print Network [OSTI]

    Buddhapriya Chakrabarti; David R. Nelson

    2009-04-09T23:59:59.000Z

    We study theoretically the mechanical failure of a simple model of double stranded DNA under an applied shear. Starting from a more microscopic Hamiltonian that describes a sheared DNA, we arrive at a nonlinear generalization of a ladder model of shear unzipping proposed earlier by deGennes [deGennes P. G. C. R. Acad. Sci., Ser. IV; Phys., Astrophys. 2001, 1505]. Using this model and a combination of analytical and numerical methods, we study the DNA "unzipping" transition when the shearing force exceeds a critical threshold at zero temperature. We also explore the effects of sequence heterogeneity and finite temperature and discuss possible applications to determine the strength of colloidal nanoparticle assemblies functionalized by DNA.

  10. Intermediate DNA at low added salt: DNA bubbles slow the diffusion of short DNA fragments

    E-Print Network [OSTI]

    Tomislav Vuletic; Sanja Dolanski Babic; Ticijana Ban; Joachim Raedler; Francoise Livolant; Silvia Tomic

    2011-01-05T23:59:59.000Z

    We report a study of DNA (150 bp fragments) conformations in very low added salt $DNA concentration range $0.0015\\leq c \\leq 8$~mM (bp). We found an intermediate DNA conformation in the region $0.05 DNA has the diffusion coefficient, $D_p$ reduced below the values for both ssDNA coils and native dsDNA helices of similar polymerization degree $N$. Thus, this DNA population can not be a simple mix of dsDNA and of ssDNA which results from DNA melting. Here, melting occurs due to a reduction in screening concomitant with DNA concentration being reduced, in already very low salt conditions. The intermediate DNA is rationalized through the well known concept of fluctuational openings (DNA bubbles) which we postulate to form in AT-rich portions of the sequence, without the strands coming apart. Within the bubbles, DNA is locally stretched, while the whole molecule remains rod-like due to very low salt environment. Therefore, such intermediate DNA is elongated, in comparison to dsDNA, which accounts for its reduced $D_p$.

  11. Strandwise translocation of a DNA glycosylase on undamaged DNA

    SciTech Connect (OSTI)

    Qi, Yan; Nam, Kwangho; Spong, Marie C.; Banerjee, Anirban; Sung, Rou-Jia; Zhang, Michael; Karplus, Martin; Verdine, Gregory L. (Harvard)

    2012-05-14T23:59:59.000Z

    Base excision repair of genotoxic nucleobase lesions in the genome is critically dependent upon the ability of DNA glycosylases to locate rare sites of damage embedded in a vast excess of undamaged DNA, using only thermal energy to fuel the search process. Considerable interest surrounds the question of how DNA glycosylases translocate efficiently along DNA while maintaining their vigilance for target damaged sites. Here, we report the observation of strandwise translocation of 8-oxoguanine DNA glycosylase, MutM, along undamaged DNA. In these complexes, the protein is observed to translocate by one nucleotide on one strand while remaining untranslocated on the complementary strand. We further report that alterations of single base-pairs or a single amino acid substitution (R112A) can induce strandwise translocation. Molecular dynamics simulations confirm that MutM can translocate along DNA in a strandwise fashion. These observations reveal a previously unobserved mode of movement for a DNA-binding protein along the surface of DNA.

  12. Coarse-graining DNA for simulations of DNA nanotechnology

    E-Print Network [OSTI]

    Doye, Jonathan P K; Louis, Ard A; Romano, Flavio; Sulc, Petr; Matek, Christian; Snodin, Benedict E K; Rovigatti, Lorenzo; Schreck, John S; Harrison, Ryan M; Smith, William P J

    2013-01-01T23:59:59.000Z

    To simulate long time and length scale processes involving DNA it is necessary to use a coarse-grained description. Here we provide an overview of different approaches to such coarse graining, focussing on those at the nucleotide level that allow the self-assembly processes associated with DNA nanotechnology to be studied. OxDNA, our recently-developed coarse-grained DNA model, is particularly suited to this task, and has opened up this field to systematic study by simulations. We illustrate some of the range of DNA nanotechnology systems to which the model is being applied, as well as the insights it can provide into fundamental biophysical properties of DNA.

  13. Hairpin DNA Switch for Ultrasensitive Spectrophotometric Detection...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Hairpin DNA Switch for Ultrasensitive Spectrophotometric Detection of DNA Hybridization Based on Gold Nanoparticles and Enzyme Hairpin DNA Switch for Ultrasensitive...

  14. DNA polymerase having modified nucleotide binding site for DNA sequencing

    DOE Patents [OSTI]

    Tabor, Stanley (Cambridge, MA); Richardson, Charles (Chestnut Hill, MA)

    1997-01-01T23:59:59.000Z

    Modified gene encoding a modified DNA polymerase wherein the modified polymerase incorporates dideoxynucleotides at least 20-fold better compared to the corresponding deoxynucleotides as compared with the corresponding naturally-occurring DNA polymerase.

  15. DNA polymerase having modified nucleotide binding site for DNA sequencing

    DOE Patents [OSTI]

    Tabor, S.; Richardson, C.

    1997-03-25T23:59:59.000Z

    A modified gene encoding a modified DNA polymerase is disclosed. The modified polymerase incorporates dideoxynucleotides at least 20-fold better compared to the corresponding deoxynucleotides as compared with the corresponding naturally-occurring DNA polymerase. 6 figs.

  16. Docking-dependent regulation of checkpoint kinase chk1 by the growth regulator p21WAF1

    E-Print Network [OSTI]

    Toh, Yew Kwang

    2009-01-01T23:59:59.000Z

    Checkpoint kinase 1 (Chk1) is a key player in the DNA damage response signalling pathway and the mode of Chk1 activation whereby it undergoes ATRdependent phosphorylation at Ser317 and Ser345 is well characterised. It ...

  17. Searching for DNA Lesions: Structural Evidence for Lower- and Higher-Affinity DNA Binding Conformations of Human Alkyladenine DNA Glycosylase

    E-Print Network [OSTI]

    Drennan, Catherine L.

    To efficiently repair DNA, human alkyladenine DNA glycosylase (AAG) must search the million-fold excess of unmodified DNA bases to find a handful of DNA lesions. Such a search can be facilitated by the ability of glycosylases, ...

  18. DNA Structural Nanotechnology Duke University

    E-Print Network [OSTI]

    Reif, John H.

    DNA Structural Nanotechnology John Reif Duke University Graduate Students: Harish Chandran&Caltech Tube Lattices #12;Ned Seeman New York University, USA Ned Seeman: Father of DNA Nanotechnology His Initial Ideas & Motivation for DNA Nanotechnology #12;Cube Chen & Seeman, Nature350:631 (1991) Truncated

  19. Sensing DNA - DNA as nanosensor: a perspective towards nanobiotechnology

    E-Print Network [OSTI]

    Ralf Metzler; Tobias Ambjoernsson

    2005-08-20T23:59:59.000Z

    Based on modern single molecule techniques, we devise a number of possible experimental setups to probe local properties of DNA such as the presence of DNA-knots, loops or folds, or to obtain information on the DNA-sequence. Similarly, DNA may be used as a local sensor. Employing single molecule fluorescence methods, we propose to make use of the physics of DNA denaturation nanoregions to find out about the solvent conditions such as ionic strength, presence of binding proteins, etc. By measuring dynamical quantities in particular, rather sensitive nanoprobes may be constructed with contemporary instruments.

  20. Exploration of the meteorological characteristics leading to the rapid cessation of cloud-to-ground lightning in winter cyclones along the East Coast of the United States

    E-Print Network [OSTI]

    Demetriades, Nicholas William Snow

    1999-01-01T23:59:59.000Z

    Page DEDICATION. V ACKNOWLEDGEMENTS. Vl TABLE OF CONTENTS. Vl1 LIST OF FIGURES. . LIST OF TABLES. CHAPTER Xlv I HUTRODUCTION. II BACKGROUND. 1. Winter Thunderstorms. . 2. Cloud Electrification-Charging Mechanisms... for the northeast quadrant of all seven East Coast cyclones. 112 7 Same as Table 6, except for the NICG lightning sounding data. . 1 13 8 Same as Table 6, except for the southeast quadrant. . . 116 9 Same as Table 7, except for the southeast quadrant. . . . 10...

  1. DNA decontamination: DNA-ExitusPlus in comparison with conventional reagents

    E-Print Network [OSTI]

    Cai, Long

    DNA decontamination: DNA-ExitusPlus in comparison with conventional reagents Here we present a completely new DNA decontamination reagent DNA-ExitusPlus. In comparison with conventional products, DNA solutions for effective DNA decontamination. DNA decontamination reagents use three different molecular prin

  2. Fleet DNA (Presentation)

    SciTech Connect (OSTI)

    Walkokwicz, K.; Duran, A.

    2014-06-01T23:59:59.000Z

    The Fleet DNA project objectives include capturing and quantifying drive cycle and technology variation for the multitude of medium- and heavy-duty vocations; providing a common data storage warehouse for medium- and heavy-duty vehicle fleet data across DOE activities and laboratories; and integrating existing DOE tools, models, and analyses to provide data-driven decision making capabilities. Fleet DNA advantages include: for Government - providing in-use data for standard drive cycle development, R&D, tech targets, and rule making; for OEMs - real-world usage datasets provide concrete examples of customer use profiles; for fleets - vocational datasets help illustrate how to maximize return on technology investments; for Funding Agencies - ways are revealed to optimize the impact of financial incentive offers; and for researchers -a data source is provided for modeling and simulation.

  3. Tops and Writhing DNA

    E-Print Network [OSTI]

    Joseph Samuel; Supurna Sinha

    2010-11-30T23:59:59.000Z

    The torsional elasticity of semiflexible polymers like DNA is of biological significance. A mathematical treatment of this problem was begun by Fuller using the relation between link, twist and writhe, but progress has been hindered by the non-local nature of the writhe. This stands in the way of an analytic statistical mechanical treatment, which takes into account thermal fluctuations, in computing the partition function. In this paper we use the well known analogy with the dynamics of tops to show that when subjected to stretch and twist, the polymer configurations which dominate the partition function admit a local writhe formulation in the spirit of Fuller and thus provide an underlying justification for the use of Fuller's "local writhe expression" which leads to considerable mathematical simplification in solving theoretical models of DNA and elucidating their predictions. Our result facilitates comparison of the theoretical models with single molecule micromanipulation experiments and computer simulations.

  4. DNA waves and water

    E-Print Network [OSTI]

    L. Montagnier; J. Aissa; E. Del Giudice; C. Lavallee; A. Tedeschi; G. Vitiello

    2010-12-23T23:59:59.000Z

    Some bacterial and viral DNA sequences have been found to induce low frequency electromagnetic waves in high aqueous dilutions. This phenomenon appears to be triggered by the ambient electromagnetic background of very low frequency. We discuss this phenomenon in the framework of quantum field theory. A scheme able to account for the observations is proposed. The reported phenomenon could allow to develop highly sensitive detection systems for chronic bacterial and viral infections.

  5. Genome-Wide Identification and 3D Modeling of Proteins involved in DNA Damage Recognition and Repair (Final Report)

    SciTech Connect (OSTI)

    Ruben A. Abagyan, PhD

    2004-04-15T23:59:59.000Z

    OAK-B135 DNA Damage Recognition and Repair (DDR and R) proteins play a critical role in cellular responses to low-dose radiation and are associated with cancer. the authors have performed a systematic, genome-wide computational analysis of genomic data for human genes involved in the DDR and R process. The significant achievements of this project include: (1) Construction of the computational pipeline for searching DDR and R genes, building and validation of 3D models of proteins involved in DDR and R; (2) Functional and structural annotation of the 3D models and generation of comprehensive lists of suggested knock-out mutations; (3) Important improvement of macromolecular docking technology and its application to predict the DNA-Protein complex conformation; (4) Development of a new algorithm for improved analysis of high-density oligonucleotide arrays for gene expression profiling; (5) Construction and maintenance of the DNA Damage Recognition and Repair Database; and (6) Producing 14 research papers (10 published and 4 in preparation).

  6. Interstrand DNA-DNA Cross-Link Formation Between Adenine Residues and Abasic Sites in Duplex DNA

    E-Print Network [OSTI]

    Gates, Kent. S.

    Interstrand DNA-DNA Cross-Link Formation Between Adenine Residues and Abasic Sites in Duplex DNA of DNA is a common event that generates an abasic (Ap) site (1). Ap sites exist as an equilibrating that can form covalent adducts with nucleophilic sites in DNA. Thus, Ap sites present a potentially

  7. Method for assaying clustered DNA damages

    DOE Patents [OSTI]

    Sutherland, Betsy M.

    2004-09-07T23:59:59.000Z

    Disclosed is a method for detecting and quantifying clustered damages in DNA. In this method, a first aliquot of the DNA to be tested for clustered damages with one or more lesion-specific cleaving reagents under conditions appropriate for cleavage of the DNA to produce single-strand nicks in the DNA at sites of damage lesions. The number average molecular length (Ln) of double stranded DNA is then quantitatively determined for the treated DNA. The number average molecular length (Ln) of double stranded DNA is also quantitatively determined for a second, untreated aliquot of the DNA. The frequency of clustered damages (.PHI..sub.c) in the DNA is then calculated.

  8. DNA attachment to support structures

    DOE Patents [OSTI]

    Balhorn, Rodney L. (Livermore, CA); Barry, Christopher H. (Fresno, CA)

    2002-01-01T23:59:59.000Z

    Microscopic beads or other structures are attached to nucleic acids (DNA) using a terminal transferase. The transferase adds labeled dideoxy nucleotide bases to the ends of linear strands of DNA. The labels, such as the antigens digoxigenin and biotin, bind to the antibody compounds or other appropriate complementary ligands, which are bound to the microscopic beads or other support structures. The method does not require the synthesis of a synthetic oligonucleotide probe. The method can be used to tag or label DNA even when the DNA has an unknown sequence, has blunt ends, or is a very large fragment (e.g., >500 kilobase pairs).

  9. A Model for Structure and Thermodynamics of ssDNA and dsDNA Near...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Structure and Thermodynamics of ssDNA and dsDNA Near a Surface:A Coarse Grained Approach. A Model for Structure and Thermodynamics of ssDNA and dsDNA Near a Surface:A Coarse...

  10. B-DNA Under Stress: Over- and Untwisting of DNA during MolecularDynami...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    B-DNA Under Stress: Over- and Untwisting of DNA during MolecularDynamics Simulations. B-DNA Under Stress: Over- and Untwisting of DNA during MolecularDynamics Simulations....

  11. Controlling DNA Methylation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOEThe Bonneville Power Administration would likeConstitution And BylawsMetal-Organic FrameworksControlling DNA

  12. Myosin IIA participates in docking of Glut4 storage vesicles with the plasma membrane in 3T3-L1 adipocytes

    SciTech Connect (OSTI)

    Chung, Le Thi Kim, E-mail: ngocanh@nutr.med.tokushima-u.ac.jp [Department of Nutrition and Metabolism, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8503 (Japan); Hosaka, Toshio [Department of Public Health and Applied Nutrition, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima (Japan)] [Department of Public Health and Applied Nutrition, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima (Japan); Harada, Nagakatsu; Jambaldorj, Bayasgalan; Fukunaga, Keiko; Nishiwaki, Yuka [Department of Nutrition and Metabolism, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8503 (Japan)] [Department of Nutrition and Metabolism, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8503 (Japan); Teshigawara, Kiyoshi [Clinical Research Center for Diabetes, Tokushima University Hospital, 2-50-1 Kuramoto-cho, Tokushima 770-8503 (Japan)] [Clinical Research Center for Diabetes, Tokushima University Hospital, 2-50-1 Kuramoto-cho, Tokushima 770-8503 (Japan); Sakai, Tohru [Department of Public Health and Applied Nutrition, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima (Japan)] [Department of Public Health and Applied Nutrition, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima (Japan); Nakaya, Yutaka [Department of Nutrition and Metabolism, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8503 (Japan)] [Department of Nutrition and Metabolism, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8503 (Japan); Funaki, Makoto, E-mail: m-funaki@clin.med.tokushima-u.ac.jp [Clinical Research Center for Diabetes, Tokushima University Hospital, 2-50-1 Kuramoto-cho, Tokushima 770-8503 (Japan)] [Clinical Research Center for Diabetes, Tokushima University Hospital, 2-50-1 Kuramoto-cho, Tokushima 770-8503 (Japan)

    2010-01-01T23:59:59.000Z

    In adipocytes and myocytes, insulin stimulation translocates glucose transporter 4 (Glut4) storage vesicles (GSVs) from their intracellular storage sites to the plasma membrane (PM) where they dock with the PM. Then, Glut4 is inserted into the PM and initiates glucose uptake into these cells. Previous studies using chemical inhibitors demonstrated that myosin II participates in fusion of GSVs and the PM and increase in the intrinsic activity of Glut4. In this study, the effect of myosin IIA on GSV trafficking was examined by knocking down myosin IIA expression. Myosin IIA knockdown decreased both glucose uptake and exposures of myc-tagged Glut4 to the cell surface in insulin-stimulated cells, but did not affect insulin signal transduction. Interestingly, myosin IIA knockdown failed to decrease insulin-dependent trafficking of Glut4 to the PM. Moreover, in myosin IIA knockdown cells, insulin-stimulated binding of GSV SNARE protein, vesicle-associated membrane protein 2 (VAMP2) to PM SNARE protein, syntaxin 4 was inhibited. These data suggest that myosin IIA plays a role in insulin-stimulated docking of GSVs to the PM in 3T3-L1 adipocytes through SNARE complex formation.

  13. Annual Report: 2010-2011 Storm Season Sampling For NON-DRY DOCK STORMWATER MONITORING FOR PUGET SOUND NAVAL SHIPYARD, BREMERTON, WA

    SciTech Connect (OSTI)

    Brandenberger, Jill M.; Metallo, David; Johnston, Robert K.; Gebhardt, Christine; Hsu, Larry

    2012-09-01T23:59:59.000Z

    This interim report summarizes the stormwater monitoring conducted for non-dry dock outfalls in both the confined industrial area and the residential areas of Naval Base Kitsap within the Puget Sound Naval Shipyard (referred to as the Shipyard). This includes the collection, analyses, and descriptive statistics for stormwater sampling conducted from November 2010 through April 2011. Seven stormwater basins within the Shipyard were sampled during at least three storm events to characterize non-dry dock stormwater discharges at selected stormwater drains located within the facility. This serves as the Phase I component of the project and Phase II is planned for the 2011-2012 storm season. These data will assist the Navy, USEPA, Ecology and other stakeholders in understanding the nature and condition of stormwater discharges from the Shipyard and inform the permitting process for new outfall discharges. The data from Phase I was compiled with current stormwater data available from the Shipyard, Sinclair/Dyes Inlet watershed, and Puget Sound in order to support technical investigations for the Draft NPDES permit. The permit would require storm event sampling at selected stormwater drains located within the Shipyard. However, the data must be considered on multiple scales to truly understand potential impairments to beneficial uses within Sinclair and Dyes Inlets.

  14. DNA-DNA interactions Helmut H Strey*t, Rudi Podgornik* ,

    E-Print Network [OSTI]

    Podgornik, Rudolf

    309 DNA-DNA interactions Helmut H Strey*t, Rudi Podgornik* , Parsegian The forces that govern DNA interactions - such as electrostatic interactions, hydration, and fluctuation forces - that treat DNA about the physical forces and energies that involve DNA molecules is to ask whether there is more to DNA

  15. Condensation of circular DNA

    E-Print Network [OSTI]

    E. L. Starostin

    2013-04-05T23:59:59.000Z

    A simple model of a circularly closed dsDNA in a poor solvent is considered as an example of a semi-flexible polymer with self-attraction. To find the ground states, the conformational energy is computed as a sum of the bending and torsional elastic components and the effective self-attraction energy. The model includes a relative orientation or sequence dependence of the effective attraction forces between different pieces of the polymer chain. Two series of conformations are analysed: a multicovered circle (a toroid) and a multifold two-headed racquet. The results are presented as a diagram of state. It is suggested that the stability of particular conformations may be controlled by proper adjustment of the primary structure. Application of the model to other semi-flexible polymers is considered.

  16. Sequence independent amplification of DNA

    DOE Patents [OSTI]

    Bohlander, Stefan K. (Chicago, IL)

    1998-01-01T23:59:59.000Z

    The present invention is a rapid sequence-independent amplification procedure (SIA). Even minute amounts of DNA from various sources can be amplified independent of any sequence requirements of the DNA or any a priori knowledge of any sequence characteristics of the DNA to be amplified. This method allows, for example the sequence independent amplification of microdissected chromosomal material and the reliable construction of high quality fluorescent in situ hybridization (FISH) probes from YACs or from other sources. These probes can be used to localize YACs on metaphase chromosomes but also--with high efficiency--in interphase nuclei.

  17. Normalized cDNA libraries

    DOE Patents [OSTI]

    Soares, M.B.; Efstratiadis, A.

    1997-06-10T23:59:59.000Z

    This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3{prime} noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library. 4 figs.

  18. Normalized cDNA libraries

    DOE Patents [OSTI]

    Soares, Marcelo B. (New York, NY); Efstratiadis, Argiris (Englewood, NJ)

    1997-01-01T23:59:59.000Z

    This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3' noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library.

  19. DNA UPTAKE BY TRANSFORMABLE BACTERIA

    SciTech Connect (OSTI)

    LACKS,S.A.

    1999-09-07T23:59:59.000Z

    The various processes of DNA uptake by cells can be categorized as: viral DNA entry, conjugation, or transformation. Within each category, a variety of mechanisms have been found. However, considerable similarities occur among the different mechanisms of conjugation and, especially, transformation. All of these natural mechanisms of DNA transfer are quite elaborate and involve multiple protein components, as the case may be, of the virus, the donor cell, and the recipient cell. The mechanisms of viral infection and conjugation will be discussed mainly with respect to their relevance to transformation.

  20. Transcriptional Control of DNA-Based Nanomachines

    E-Print Network [OSTI]

    Ludwig-Maximilians-Universitt, Mnchen

    on responses to stimuli or the cell's environ- ment. So far, nanomachines have been controlled with DNA rather be performed by these devices in well-defined steps. Recently, a DNA machine that binds, carries, and releases the manual addition of a "fuel" strand, consisting of single stranded DNA (ssDNA) that affects

  1. Unnatural nucleotides for DNA sequencing

    E-Print Network [OSTI]

    Jacutin, Swanee E

    1997-01-01T23:59:59.000Z

    Fluorescent nucleotide analogs were prepared and tested to find surrogate structures that are: (i) incorporated by DNA polymerases; (ii) spectroscopically distinct for each fluorescent tag; and (iii) easily deprotected at the 3'-position under mild...

  2. Mammalian DNA Repair. Final Report

    SciTech Connect (OSTI)

    None

    2003-01-24T23:59:59.000Z

    The Gordon Research Conference (GRC) on Mammalian DNA Repair was held at Harbortown Resort, Ventura Beach, CA. Emphasis was placed on current unpublished research and discussion of the future target areas in this field.

  3. Ancient DNA Chronology within Sediment Deposits: Are Paleobiological Reconstructions Possible and Is DNA Leaching a Factor?

    E-Print Network [OSTI]

    Shapiro, Beth

    reported the successful extraction of ancient DNA (aDNA) from both frozen and nonfrozen sediments (even for vertical migration of aDNA across strata. To assess the extent of this problem, we extracted aDNA from (Lydolph et al. 2005). Also uncertain is whether the DNA is extracellular and bound to clay minerals

  4. Meta-DNA: A DNA-Based Approach to Synthetic Harish Chandran1

    E-Print Network [OSTI]

    Reif, John H.

    Meta-DNA: A DNA-Based Approach to Synthetic Biology Harish Chandran1 harish@cs.duke.edu Nikhil taken here is to develop a biochemical system which we call meta-DNA (abbre- viated as mDNA), based entirely on strands of DNA as the only component molecules. Our work leverages prior work

  5. Meta-DNA: Synthetic Biology via DNA Nanostructures and Hybridization Reactions

    E-Print Network [OSTI]

    Reif, John H.

    Meta-DNA: Synthetic Biology via DNA Nanostructures and Hybridization Reactions Harish Chandran for desired functionality. The approach of this paper is to develop a biochemical system which we call meta-DNA (abbreviated as mDNA), based entirely on strands of DNA as the only component molecule. Our work leverages

  6. Impact of DNA Twist Accumulation on Progressive Helical Wrapping of Torsionally Constrained DNA

    E-Print Network [OSTI]

    Wang, Wei Hua

    Impact of DNA Twist Accumulation on Progressive Helical Wrapping of Torsionally Constrained DNA Wei (Received 17 July 2012; published 20 November 2012) DNA wrapping is an important mechanism for chromosomal DNA packaging in cells and viruses. Previous studies of DNA wrapping have been performed mostly

  7. DNA Word Design Strategy for Creating Sets of Non-interacting Oligonucleotides for DNA Microarrays

    E-Print Network [OSTI]

    DNA Word Design Strategy for Creating Sets of Non-interacting Oligonucleotides for DNA Microarrays-interacting DNA oligonucleotides for applications in DNA arrays and biosensors is demonstrated. This strategy mismatches with the complements of all the other members in the set. These "DNA word" sets are denoted as nbm

  8. DNA polymorphism identity determination using flow cytometry

    DOE Patents [OSTI]

    Nolan, John P. (Santa Fe, NM); White, P. Scott (Los Alamos, NM); Cai, Hong (Los Alamos, NM)

    2001-01-01T23:59:59.000Z

    DNA polymorphism identity determination using flow cytometry. Primers designed to be immobilized on microspheres are allowed to anneal to the DNA strand under investigation, and are extended by either DNA polymerase using fluorescent dideoxynucleotides or ligated by DNA ligase to fluorescent reporter oligonucleotides. The fluorescence of either the dideoxynucleotide or the reporter oligonucleotide attached to the immobilized primer is measured by flow cytometry, thereby identifying the nucleotide polymorphism on the DNA strand.

  9. Cellular responses to environmental DNA damage

    SciTech Connect (OSTI)

    Not Available

    1994-08-01T23:59:59.000Z

    This volume contains the proceedings of the conference entitled Cellular Responses to Environmental DNA Damage held in Banff,Alberta December 1--6, 1991. The conference addresses various aspects of DNA repair in sessions titled DNA repair; Basic Mechanisms; Lesions; Systems; Inducible Responses; Mutagenesis; Human Population Response Heterogeneity; Intragenomic DNA Repair Heterogeneity; DNA Repair Gene Cloning; Aging; Human Genetic Disease; and Carcinogenesis. Individual papers are represented as abstracts of about one page in length.

  10. Regulation of DNA damage tolerance : studies of the translesion synthesis DNA ploymerase eta in Saccharomyces cerevisiae

    E-Print Network [OSTI]

    Woodruff, Rachel Van Etten

    2008-01-01T23:59:59.000Z

    All organisms must control the effects of DNA damage to protect the integrity of their genomes. In addition to DNA repair, this requires DNA damage tolerance pathways, which allow the continuation of essential processes ...

  11. DNA Repair Decline During Mouse Spermiogenesis Results in the Accumulation of Heritable DNA Damage

    E-Print Network [OSTI]

    Marchetti, Francesco

    2008-01-01T23:59:59.000Z

    van Buul, D.G. de Rooij, DNA double-strand breaks and gamma-for the transition proteins in DNA strand break repair, FEBSBoissonneault, Stimulation of DNA repair by the spermatidal

  12. DNA Strands Attached Inside Single Conical Nanopores: Ionic Pore Characteristics and Insight into DNA Biophysics

    E-Print Network [OSTI]

    Nguyen, Gael; Howorka, Stefan; Siwy, Zuzanna S.

    2011-01-01T23:59:59.000Z

    Nonexponential kinetics of DNA escape from alpha-hemolysin2010), (iii) the speed of DNA transport (Meller et al. 2001;0AJ, UK G. Nguyen et al. : DNA Strands in Single Nanopores

  13. DNA binding specificity of the p73 DNA-binding domain

    E-Print Network [OSTI]

    Tse, Pui Wah

    2011-01-01T23:59:59.000Z

    interactions of p53 with DNA: when flexibility serves2006). Structural basis of DNA recognition by p53 tetramers.Z. (2010). Diversity in DNA recognition by p53 revealed by

  14. DNA repair decline during mouse spermiogenesis results in the accumulation of heritable DNA damage

    E-Print Network [OSTI]

    Marchetti, Francesco

    2008-01-01T23:59:59.000Z

    van Buul, D.G. de Rooij, DNA double-strand breaks and gamma-for the transition proteins in DNA strand break repair, FEBSBoissonneault, Stimulation of DNA repair by the spermatidal

  15. Quantitative measurement and modeling of the DNA damage signaling network : DNA double-strand breaks

    E-Print Network [OSTI]

    Tentner, Andrea R. (Andrea Ruth)

    2009-01-01T23:59:59.000Z

    DNA double-strand breaks (DSB) are one of the major mediators of chemotherapy-induced cytotoxicity in tumors. Cells that experience DNA damage can initiate a DNA damage-mediated cell-cycle arrest, attempt to repair the ...

  16. DNA repair decline during mouse spermiogenesis results in the accumulation of heritable DNA damage

    E-Print Network [OSTI]

    Marchetti, Francesco

    2008-01-01T23:59:59.000Z

    male germ cells handle DNA damage? Toxicol. Appl. Pharmacol.strand breaks and DNA base damage at different cellularrelationship to genetic damage, Mutat. Res. 216 (1989) 221-

  17. DNA Repair Decline During Mouse Spermiogenesis Results in the Accumulation of Heritable DNA Damage

    E-Print Network [OSTI]

    Marchetti, Francesco

    2008-01-01T23:59:59.000Z

    male germ cells handle DNA damage? Toxicol. Appl. Pharmacol.strand breaks and DNA base damage at different cellularrelationship to genetic damage, Mutat. Res. 216 (1989) 221-

  18. DNA repair is the target of novel antibiotics

    E-Print Network [OSTI]

    Gunderson, Carl Wayne

    2007-01-01T23:59:59.000Z

    W. & A. M. Segall, (2006) DNA repair, a novel antibacterialjunction-trapping peptides induce DNA damage and chromosomePeptide inhibitors of DNA cleavage by tyrosine recombinases

  19. DNA Guided Self-Assembly of Nanocrystals for Optoelectronic Devices /

    E-Print Network [OSTI]

    Noh, Hyunwoo

    2013-01-01T23:59:59.000Z

    Lithographically Confined DNA Origami. Nat. Nanotech. 2010,and Orientation of Individual DNA Shapes on LithographicallyB. ; Yan, H. ; Liu, Y. DNA-Origami-Directed Self- Assembly

  20. GRED Studies and Drilling of Americulture State 2, Americulture...

    Open Energy Info (EERE)

    and Drilling of Americulture State 2, Americulture Tilapia Farm: Lightning Dock KGRA, Las Animas Valley, New Mexico Jump to: navigation, search OpenEI Reference LibraryAdd to...

  1. Development of a plan to implement enhanced geothermal system...

    Open Energy Info (EERE)

    injection to the production well. Authors D.N. Schochet and R.A. Cunniff Published ORMAT International, Inc. and Lightning Dock Geothermal, Inc., 2001 DOI Not Provided Check for...

  2. High-Fidelity DNA Hybridization Using Programmable Molecular DNA Devices

    E-Print Network [OSTI]

    Reif, John H.

    of complementary nucleic acid strands is the most basic of all reactions involving nucleic acids, but has a major specific high-fidelity DNA hybridization reactions for tar- get strands of arbitrary length. Our protocol acid strands is the most basic of all reactions involving nucleic acids and a major component of most

  3. Micropatterned cell arrays for detecting DNA damage

    E-Print Network [OSTI]

    Mittal, Sukant

    2008-01-01T23:59:59.000Z

    Numerous agents are capable of interacting with DNA and damaging it. Permanent changes in the DNA structure can be both mutagenic and cytotoxic; therefore, methods to measure the susceptibility of cells to mutations are ...

  4. Towards Privacy Preserving of Forensic DNA Databases

    E-Print Network [OSTI]

    Liu, Sanmin

    2012-02-14T23:59:59.000Z

    Protecting privacy of individuals is critical for forensic genetics. In a kinship/identity testing, related DNA profiles between user's query and the DNA database need to be extracted. However, unrelated profiles cannot be revealed to each other...

  5. Amplification of chromosomal DNA in situ

    DOE Patents [OSTI]

    Christian, Allen T. (Tracy, CA); Coleman, Matthew A. (Livermore, CA); Tucker, James D. (Livermore, CA)

    2002-01-01T23:59:59.000Z

    Amplification of chromosomal DNA in situ to increase the amount of DNA associated with a chromosome or chromosome region is described. The amplification of chromosomal DNA in situ provides for the synthesis of Fluorescence in situ Hybridization (FISH) painting probes from single dissected chromosome fragments, the production of cDNA libraries from low copy mRNAs and improved in Comparative Genomic Hybridization (CGH) procedures.

  6. DNA Microarrays An R Tutorial

    E-Print Network [OSTI]

    Qiu, Weigang

    Analysis & R Tutorial #12;DNA Microarrays An R Tutorial R: Graphics and Statistics beyond Excel R: an Open Source statistical package (http://www.r-project.org) RStudio: a Graphic User Interface to R (http(stock) # median > range(stock) # mim and max > sum(stock) # sum > var(stock) # variance > sd(stock) # standard

  7. Chromosome specific repetitive DNA sequences

    DOE Patents [OSTI]

    Moyzis, Robert K. (Los Alamos, NM); Meyne, Julianne (Los Alamos, NM)

    1991-01-01T23:59:59.000Z

    A method is provided for determining specific nucleotide sequences useful in forming a probe which can identify specific chromosomes, preferably through in situ hybridization within the cell itself. In one embodiment, chromosome preferential nucleotide sequences are first determined from a library of recombinant DNA clones having families of repetitive sequences. Library clones are identified with a low homology with a sequence of repetitive DNA families to which the first clones respectively belong and variant sequences are then identified by selecting clones having a pattern of hybridization with genomic DNA dissimilar to the hybridization pattern shown by the respective families. In another embodiment, variant sequences are selected from a sequence of a known repetitive DNA family. The selected variant sequence is classified as chromosome specific, chromosome preferential, or chromosome nonspecific. Sequences which are classified as chromosome preferential are further sequenced and regions are identified having a low homology with other regions of the chromosome preferential sequence or with known sequences of other family me This invention is the result of a contract with the Department of Energy (Contract No. W-7405-ENG-36).

  8. Protein-DNA Interactions Determine the Shapes of DNA Toroids Condensed in Virus Capsids

    E-Print Network [OSTI]

    Podgornik, Rudolf

    Protein-DNA Interactions Determine the Shapes of DNA Toroids Condensed in Virus Capsids Ame, University of Ljubljana, Ljubljana, Slovenia ABSTRACT DNA toroids that form inside the bacteriophage capsid glycol to the bathing solution. Spermine-DNA toroids present a convex, faceted section with no or minor

  9. Probe and method for DNA detection

    SciTech Connect (OSTI)

    Yeh, Hsin-Chih; Werner, James Henry; Sharma, Jaswinder Kumar; Martinez, Jennifer Suzanne

    2013-07-02T23:59:59.000Z

    A hybridization probe containing two linear strands of DNA lights up upon hybridization to a target DNA using silver nanoclusters that have been templated onto one of the DNA strands. Hybridization induces proximity between the nanoclusters on one strand and an overhang on the other strand, which results in enhanced fluorescence emission from the nanoclusters.

  10. DNA Sequencing via Electron Tunneling Michael Zwolak

    E-Print Network [OSTI]

    Zwolak, Michael

    DNA Sequencing via Electron Tunneling Michael Zwolak Department of Physics Oregon State University-cost DNA sequencing methods would revolutionize medicine: a person could have his/her full genome sequenced of "personalized medicine" is hampered today by the high cost and slow speed of DNA sequencing methods. We

  11. Antibody specific for a DNA repair protein

    DOE Patents [OSTI]

    Petrini, John H.; Morgan, William Francis; Maser, Richard Scott; Carney, James Patrick

    2006-07-11T23:59:59.000Z

    An isolated and purified DNA molecule encoding a DNA repair protein, p95, is provided, as is isolated and purified p95. Also provided are methods of detecting p95 and DNA encoding p95. The invention further provides p95 knock-out mice.

  12. DNA and the Genetic Code June 14, 2011

    E-Print Network [OSTI]

    Guevara-Vasquez, Fernando

    DNA and the Genetic Code June 14, 2011 DNA and the Genetic Code #12;Protein synthesis The genetic synthesis requires two steps: transcription and translation. DNA and the Genetic Code #12;DNA DNA development. DNA is comprised of 4 bases: guanine (G), adenine (A), cytosine (C), and thymine (T). The bases

  13. Method for sequencing DNA base pairs

    DOE Patents [OSTI]

    Sessler, Andrew M. (Oakland, CA); Dawson, John (Pacific Palisades, CA)

    1993-01-01T23:59:59.000Z

    The base pairs of a DNA structure are sequenced with the use of a scanning tunneling microscope (STM). The DNA structure is scanned by the STM probe tip, and, as it is being scanned, the DNA structure is separately subjected to a sequence of infrared radiation from four different sources, each source being selected to preferentially excite one of the four different bases in the DNA structure. Each particular base being scanned is subjected to such sequence of infrared radiation from the four different sources as that particular base is being scanned. The DNA structure as a whole is separately imaged for each subjection thereof to radiation from one only of each source.

  14. Binary electrokinetic separation of target DNA from background DNA primers.

    SciTech Connect (OSTI)

    James, Conrad D.; Derzon, Mark Steven

    2005-10-01T23:59:59.000Z

    This report contains the summary of LDRD project 91312, titled ''Binary Electrokinetic Separation of Target DNA from Background DNA Primers''. This work is the first product of a collaboration with Columbia University and the Northeast BioDefense Center of Excellence. In conjunction with Ian Lipkin's lab, we are developing a technique to reduce false positive events, due to the detection of unhybridized reporter molecules, in a sensitive and multiplexed detection scheme for nucleic acids developed by the Lipkin lab. This is the most significant problem in the operation of their capability. As they are developing the tools for rapidly detecting the entire panel of hemorrhagic fevers this technology will immediately serve an important national need. The goal of this work was to attempt to separate nucleic acid from a preprocessed sample. We demonstrated the preconcentration of kilobase-pair length double-stranded DNA targets, and observed little preconcentration of 60 base-pair length single-stranded DNA probes. These objectives were accomplished in microdevice formats that are compatible with larger detection systems for sample pre-processing. Combined with Columbia's expertise, this technology would enable a unique, fast, and potentially compact method for detecting/identifying genetically-modified organisms and multiplexed rapid nucleic acid identification. Another competing approach is the DARPA funded IRIS Pharmaceutical TIGER platform which requires many hours for operation, and an 800k$ piece of equipment that fills a room. The Columbia/SNL system could provide a result in 30 minutes, at the cost of a few thousand dollars for the platform, and would be the size of a shoebox or smaller.

  15. Microfluidic DNA sample preparation method and device

    DOE Patents [OSTI]

    Krulevitch, Peter A. (Pleasanton, CA); Miles, Robin R. (Danville, CA); Wang, Xiao-Bo (San Diego, CA); Mariella, Raymond P. (Danville, CA); Gascoyne, Peter R. C. (Bellaire, TX); Balch, Joseph W. (Livermore, CA)

    2002-01-01T23:59:59.000Z

    Manipulation of DNA molecules in solution has become an essential aspect of genetic analyses used for biomedical assays, the identification of hazardous bacterial agents, and in decoding the human genome. Currently, most of the steps involved in preparing a DNA sample for analysis are performed manually and are time, labor, and equipment intensive. These steps include extraction of the DNA from spores or cells, separation of the DNA from other particles and molecules in the solution (e.g. dust, smoke, cell/spore debris, and proteins), and separation of the DNA itself into strands of specific lengths. Dielectrophoresis (DEP), a phenomenon whereby polarizable particles move in response to a gradient in electric field, can be used to manipulate and separate DNA in an automated fashion, considerably reducing the time and expense involved in DNA analyses, as well as allowing for the miniaturization of DNA analysis instruments. These applications include direct transport of DNA, trapping of DNA to allow for its separation from other particles or molecules in the solution, and the separation of DNA into strands of varying lengths.

  16. Fleet DNA Project (Fact Sheet)

    SciTech Connect (OSTI)

    Not Available

    2012-10-01T23:59:59.000Z

    The Fleet DNA Project - designed by the U.S. Department of Energy's National Renewable Energy Laboratory (NREL) in partnership with Oak Ridge National Laboratory - aims to accelerate the evolution of advanced vehicle development and support the strategic deployment of market-ready technologies that reduce costs, fuel consumption, and emissions. At the heart of the Fleet DNA Project is a clearinghouse of medium- and heavy-duty commercial fleet transportation data for optimizing the design of advanced vehicle technologies or for selecting a given technology to invest in. An easy-to-access online database will help vehicle manufacturers and fleets understand the broad operational range for many of today's commercial vehicle vocations.

  17. Particle sizer and DNA sequencer

    DOE Patents [OSTI]

    Olivares, Jose A.; Stark, Peter C.

    2005-09-13T23:59:59.000Z

    An electrophoretic device separates and detects particles such as DNA fragments, proteins, and the like. The device has a capillary which is coated with a coating with a low refractive index such as Teflon.RTM. AF. A sample of particles is fluorescently labeled and injected into the capillary. The capillary is filled with an electrolyte buffer solution. An electrical field is applied across the capillary causing the particles to migrate from a first end of the capillary to a second end of the capillary. A detector light beam is then scanned along the length of the capillary to detect the location of the separated particles. The device is amenable to a high throughput system by providing additional capillaries. The device can also be used to determine the actual size of the particles and for DNA sequencing.

  18. Channel plate for DNA sequencing

    DOE Patents [OSTI]

    Douthart, R.J.; Crowell, S.L.

    1998-01-13T23:59:59.000Z

    This invention is a channel plate that facilitates data compaction in DNA sequencing. The channel plate has a length, a width and a thickness, and further has a plurality of channels that are parallel. Each channel has a depth partially through the thickness of the channel plate. Additionally an interface edge permits electrical communication across an interface through a buffer to a deposition membrane surface. 15 figs.

  19. The dynamic interplay between DNA damage and metabolism : the metabolic fate and transport of DNA lesions and novel DNA damage derived from intermediary metabolism

    E-Print Network [OSTI]

    Jumpathong, Watthanachai

    2014-01-01T23:59:59.000Z

    The work presented in this thesis explores two novel and complementary facets of endogenous DNA damage: the development of biomarkers of inflammation based on metabolites of DNA damage products and the formation of DNA ...

  20. DNA Bubble Life Time in Denaturation

    E-Print Network [OSTI]

    Zh. S. Gevorkian; Chin-Kun Hu

    2010-10-11T23:59:59.000Z

    We have investigated the denaturation bubble life time for a homogeneous as well as for a heterogeneous DNA within a Poland-Scheraga model. It is shown that at criticality the bubble life time for a homogeneous DNA is finite provided that the loop entropic exponent c>2 and has a scaling dependence on DNA length for c<2. Heterogeneity in the thermodynamical limit makes the bubble life time infinite for any entropic exponent.

  1. Assembling semiconductor nanocomposites using DNA replication technologies.

    SciTech Connect (OSTI)

    Heimer, Brandon W.; Crown, Kevin K.; Bachand, George David

    2005-11-01T23:59:59.000Z

    Deoxyribonucleic acid (DNA) molecules represent Nature's genetic database, encoding the information necessary for all cellular processes. From a materials engineering perspective, DNA represents a nanoscale scaffold with highly refined structure, stability across a wide range of environmental conditions, and the ability to interact with a range of biomolecules. The ability to mass-manufacture functionalized DNA strands with Angstrom-level resolution through DNA replication technology, however, has not been explored. The long-term goal of the work presented in this report is focused on exploiting DNA and in vitro DNA replication processes to mass-manufacture nanocomposite materials. The specific objectives of this project were to: (1) develop methods for replicating DNA strands that incorporate nucleotides with ''chemical handles'', and (2) demonstrate attachment of nanocrystal quantum dots (nQDs) to functionalized DNA strands. Polymerase chain reaction (PCR) and primer extension methodologies were used to successfully synthesize amine-, thiol-, and biotin-functionalized DNA molecules. Significant variability in the efficiency of modified nucleotide incorporation was observed, and attributed to the intrinsic properties of the modified nucleotides. Noncovalent attachment of streptavidin-coated nQDs to biotin-modified DNA synthesized using the primer extension method was observed by epifluorescence microscopy. Data regarding covalent attachment of nQDs to amine- and thiol-functionalized DNA was generally inconclusive; alternative characterization tools are necessary to fully evaluate these attachment methods. Full realization of this technology may facilitate new approaches to manufacturing materials at the nanoscale. In addition, composite nQD-DNA materials may serve as novel recognition elements in sensor devices, or be used as diagnostic tools for forensic analyses. This report summarizes the results obtained over the course of this 1-year project.

  2. Chemical biology of mutagenesis and DNA repair: cellular responses to DNA alkylation

    E-Print Network [OSTI]

    Shrivastav, Nidhi

    The reaction of DNA-damaging agents with the genome results in a plethora of lesions, commonly referred to as adducts. Adducts may cause DNA to mutate, they may represent the chemical precursors of lethal events and they ...

  3. DNA Directed Assembly Probe for Detecting DNA-Protein Interaction in Microarray Format

    E-Print Network [OSTI]

    Ng, Jin Kiat

    Quantifying DNA-protein interaction using DNA microarrays are gaining increasing attention due to their ability to profile specificity of interactions in a high-throughput manner. This paper describes a new approach that ...

  4. Optical Recognition of Converted DNA Nucleotides for Single-Molecule DNA

    E-Print Network [OSTI]

    Optical Recognition of Converted DNA Nucleotides for Single-Molecule DNA Sequencing Using Nanopore among individual nucleotides (nt). The system must be capable of differentiating among the four bases

  5. DNA binding specificity of the p73 DNA-binding domain

    E-Print Network [OSTI]

    Tse, Pui Wah

    2011-01-01T23:59:59.000Z

    of DNA recognition by p53 tetramers. Mol Cell 22, 741-753.site as a self-assembled tetramer. Structure 18, 246- Chene,structure of a p53 core tetramer bound to DNA. Oncogene 28,

  6. Enhancing the DNA Patent Database

    SciTech Connect (OSTI)

    Walters, LeRoy B.

    2008-02-18T23:59:59.000Z

    Final Report on Award No. DE-FG0201ER63171 Principal Investigator: LeRoy B. Walters February 18, 2008 This project successfully completed its goal of surveying and reporting on the DNA patenting and licensing policies at 30 major U.S. academic institutions. The report of survey results was published in the January 2006 issue of Nature Biotechnology under the title The Licensing of DNA Patents by US Academic Institutions: An Empirical Survey. Lori Pressman was the lead author on this feature article. A PDF reprint of the article will be submitted to our Program Officer under separate cover. The project team has continued to update the DNA Patent Database on a weekly basis since the conclusion of the project. The database can be accessed at dnapatents.georgetown.edu. This database provides a valuable research tool for academic researchers, policymakers, and citizens. A report entitled Reaping the Benefits of Genomic and Proteomic Research: Intellectual Property Rights, Innovation, and Public Health was published in 2006 by the Committee on Intellectual Property Rights in Genomic and Protein Research and Innovation, Board on Science, Technology, and Economic Policy at the National Academies. The report was edited by Stephen A. Merrill and Anne-Marie Mazza. This report employed and then adapted the methodology developed by our research project and quoted our findings at several points. (The full report can be viewed online at the following URL: http://www.nap.edu/openbook.php?record_id=11487&page=R1). My colleagues and I are grateful for the research support of the ELSI program at the U.S. Department of Energy.

  7. DNA: The Strand that Connects Us All

    SciTech Connect (OSTI)

    Kaplan, Matt (University of Arizona Genetics Core) [University of Arizona Genetics Core

    2011-03-29T23:59:59.000Z

    Learn how the methods and discoveries of human population genetics are applied for personal genealogical reconstruction and anthropological testing. Dr. Kaplan starts with a short general review of human genetics and the biology behind this form of DNA testing. He looks at how DNA testing is performed and how samples are processed in the University of Arizona laboratory. He also examines examples of personal genealogical results from Family Tree DNA and personal anthropological results from the Genographic Project. Finally, he describes the newest project in the UA laboratory, the DNA Shoah Project.

  8. SnapShot: DNA Polymerases II Mammals

    E-Print Network [OSTI]

    Foti, James J.

    DNA polymerases ensure the faithful duplication of genetic information inside the nuclease and mitochondria of eukaryotic cells and the nucleoid of prokaryotic cells. These remarkable enzymes synthesize polynucleotide ...

  9. DNA sequencing using fluorescence background electroblotting membrane

    DOE Patents [OSTI]

    Caldwell, Karin D. (Salt Lake City, UT); Chu, Tun-Jen (Salt Lake City, UT); Pitt, William G. (Orem, UT)

    1992-01-01T23:59:59.000Z

    A method for the multiplex sequencing on DNA is disclosed which comprises the electroblotting or specific base terminated DNA fragments, which have been resolved by gel electrophoresis, onto the surface of a neutral non-aromatic polymeric microporous membrane exhibiting low background fluorescence which has been surface modified to contain amino groups. Polypropylene membranes are preferably and the introduction of amino groups is accomplished by subjecting the membrane to radio or microwave frequency plasma discharge in the presence of an aminating agent, preferably ammonia. The membrane, containing physically adsorbed DNA fragments on its surface after the electroblotting, is then treated with crosslinking means such as UV radiation or a glutaraldehyde spray to chemically bind the DNA fragments to the membrane through said smino groups contained on the surface thereof. The DNA fragments chemically bound to the membrane are subjected to hybridization probing with a tagged probe specific to the sequence of the DNA fragments. The tagging may be by either fluorophores or radioisotopes. The tagged probes hybridized to said target DNA fragments are detected and read by laser induced fluorescence detection or autoradiograms. The use of aminated low fluorescent background membranes allows the use of fluorescent detection and reading even when the available amount of DNA to be sequenced is small. The DNA bound to the membrances may be reprobed numerous times.

  10. Nucleotide capacitance calculation for DNA sequencing

    SciTech Connect (OSTI)

    Lu, Jun-Qiang [ORNL; Zhang, Xiaoguang [ORNL

    2008-01-01T23:59:59.000Z

    Using a first-principles linear response theory, the capacitance of the DNA nucleotides, adenine, cytosine, guanine and thymine, are calculated. The difference in the capacitance between the nucleotides is studied with respect to conformational distortion. The result suggests that although an alternate current capacitance measurement of a single-stranded DNA chain threaded through a nano-gap electrodes may not sufficient to be used as a stand alone method for rapid DNA sequencing, the capacitance of the nucleotides should be taken into consideration in any GHz-frequency electric measurements and may also serve as an additional criterion for identifying the DNA sequence.

  11. DNA-guided nanoparticle assemblies

    DOE Patents [OSTI]

    Gang, Oleg; Nykypanchuk, Dmytro; Maye, Mathew; van der Lelie, Daniel

    2013-07-16T23:59:59.000Z

    In some embodiments, DNA-capped nanoparticles are used to define a degree of crystalline order in assemblies thereof. In some embodiments, thermodynamically reversible and stable body-centered cubic (bcc) structures, with particles occupying <.about.10% of the unit cell, are formed. Designs and pathways amenable to the crystallization of particle assemblies are identified. In some embodiments, a plasmonic crystal is provided. In some aspects, a method for controlling the properties of particle assemblages is provided. In some embodiments a catalyst is formed from nanoparticles linked by nucleic acid sequences and forming an open crystal structure with catalytically active agents attached to the crystal on its surface or in interstices.

  12. DNA

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    algorithms that we have developed to see the inner workings of molecular machines," said Thomas Terwilliger, a senior Los Alamos scientist and Laboratory Fellow. In this case,...

  13. DNA

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOEThe Bonneville Power Administration wouldDECOMPOSITION OF CALCIUM SULFATE: A REVIEWThis rcportJ it cdrives

  14. DNA nanotechnology DOI: 10.1002/smll.200500464

    E-Print Network [OSTI]

    Li, Jiali

    DNA nanotechnology DOI: 10.1002/smll.200500464 Towards Rapid DNA Sequencing: Detecting Single- Stranded DNA with a Solid-State Nanopore Hao Yan* and Bingqian Xu* Keywords: DNA sequencing single for rapid detection of single DNA molecules and their sequences. Two types of nanopores have been used

  15. Sequence specific alkylation of DNA by hairpin pyrroleimidazole polyamide conjugates

    E-Print Network [OSTI]

    Stoltz, Brian M.

    Sequence specific alkylation of DNA by hairpin pyrroleimidazole polyamide conjugates Nicholas R predetermined sequences in the minor groove of DNA with affinities and specificities comparable to those of DNA for covalent reaction at specific DNA sequences and thereby inhibit DNAprotein interactions. Site

  16. Sequence Recognition of DNA by Protein-Induced Conformational Transitions

    E-Print Network [OSTI]

    Williams, Loren

    Sequence Recognition of DNA by Protein-Induced Conformational Transitions Derrick Watkins1 The binding of proteins to specific sequences of DNA is an important feature of virtually all DNA transactions. Proteins recognize specific DNA sequences using both direct readout (sensing types and positions of DNA

  17. DNA Profiling Using Solid-State Nanopores: Detection of DNA-Binding

    E-Print Network [OSTI]

    Meller, Amit

    a 3.5 nm pore results from threading of a dye-intercalated DNA molecule, as compared to the typical for drug development, necessitating new in vitro methods for rapid and low-cost assessment of the binding molecules, which give the DNA/intercalator complex a bulkier structure than that of native DNA. Furthermore

  18. A DNA Based Implementation of an Evolutionary Search for Good Encodings for DNA Computation

    E-Print Network [OSTI]

    Deaton, Russell J.

    lelism, capacity, and power. This potential, however, is limited by the constraints imposed by the DNA chemistry 1]. Adleman 2] introduced a way to solve combina- torial optimization problems with DNA- mental reaction in DNA based computation is hydro- gen bonding between Watson-Crick complement base pairs

  19. alkyladenine dna glycosylase: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    recognition and repair by 3-methyladenine DNA glycosylase I (TAG) Audrey H Metz1II), Helicobacter pylori 3mA DNA glycosylase (MagIII), yeast methyladenine DNA glycosylase...

  20. Single Stranded DNA Induced Assembly of Gold Nanoparticles

    E-Print Network [OSTI]

    Yang, Jun

    The binding affinity of single stranded DNA (ssDNA) for gold nanoparticle surface is studied in this work. The data indicate that the strength of interaction between ssDNA and Au particle surface is closely related to the ...

  1. Single molecule analysis of DNA electrophoresis in microdevices

    E-Print Network [OSTI]

    Randall, Greg C

    2006-01-01T23:59:59.000Z

    Given that current electrophoresis technology is inadequate for mapping large O[100 kilobasepair] DNA, several promising lab-on-chip designs for DNA mapping have been recently proposed that require either 1) a DNA molecule ...

  2. DNA topology confers sequence specificity to nonspecific architectural proteins

    E-Print Network [OSTI]

    Swigon, David

    DNA topology confers sequence specificity to nonspecific architectural proteins Juan Weia , Luke of DNA change the disorder found in chain molecules randomly decorated by nonspecific, architectural, counter to expectations, in greater quantities and at particular sites along simulated DNA minicircles

  3. DNA Damage, Repair & Replication Using E. Coli Model Systems

    E-Print Network [OSTI]

    Troll, Christopher

    2013-01-01T23:59:59.000Z

    431. Kunkel, T.A. (2004) DNA replication fidelity. J Biol1996) Generation of an endogenous DNA- methylating agent bypombe Mag1 alkylpurine DNA glycosylase. EMBO Rep, 12, 1286-

  4. Electrokinetic Concentration of DNA Polymers in Nanofluidic Channels

    E-Print Network [OSTI]

    Dekker, Cees

    Electrokinetic Concentration of DNA Polymers in Nanofluidic Channels Derek Stein,, Zeno Deurvorst on this understanding by demonstrating how a nanofluidic device with integrated electrodes can preconcentrate DNA. KEYWORDS Nanofluidic, DNA, electrokinetic, concentration M iniature fluidic devices are having an important

  5. Allostery through protein-induced DNA bubbles

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Traverso, Joseph J.; Manoranjan, Valipuram S.; Bishop, A. R.; Rasmussen, Kim .; Voulgarakis, Nikolaos K.

    2015-03-12T23:59:59.000Z

    Allostery through DNA is increasingly recognized as an important modulator of DNA functions. Here, we show that the coalescence of protein-induced DNA bubbles can mediate allosteric interactions that drive protein aggregation. We propose that such allostery may regulate DNA's flexibility and the assembly of the transcription machinery. Mitochondrial transcription factor A (TFAM), a dual-function protein involved in mitochondrial DNA (mtDNA) packaging and transcription initiation, is an ideal candidate to test such a hypothesis owing to its ability to locally unwind the double helix. Numerical simulations demonstrate that the coalescence of TFAM-induced bubbles can explain experimentally observed TFAM oligomerization. The resultingmoremelted DNA segment, approximately 10 base pairs long, around the joints of the oligomers act as flexible hinges, which explains the efficiency of TFAM in compacting DNA. Since mitochondrial polymerase (mitoRNAP) is involved in melting the transcription bubble, TFAM may use the same allosteric interaction to both recruit mitoRNAP and initiate transcription.less

  6. Recombinant DNA encoding a desulfurization biocatalyst

    DOE Patents [OSTI]

    Rambosek, John (Seattle, WA); Piddington, Chris S. (Seattle, WA); Kovacevich, Brian R. (Seattle, WA); Young, Kevin D. (Grand Forks, ND); Denome, Sylvia A. (Thompson, ND)

    1994-01-01T23:59:59.000Z

    This invention relates to a recombinant DNA molecule containing a gene or genes which encode a biocatalyst capable of desulfurizing a fossil fuel which contains organic sulfur molecules. For example, the present invention encompasses a recombinant DNA molecule containing a gene or genes of a strain of Rhodococcus rhodochrous.

  7. Recombinant DNA encoding a desulfurization biocatalyst

    DOE Patents [OSTI]

    Rambosek, J.; Piddington, C.S.; Kovacevich, B.R.; Young, K.D.; Denome, S.A.

    1994-10-18T23:59:59.000Z

    This invention relates to a recombinant DNA molecule containing a gene or genes which encode a biocatalyst capable of desulfurizing a fossil fuel which contains organic sulfur molecules. For example, the present invention encompasses a recombinant DNA molecule containing a gene or genes of a strain of Rhodococcus rhodochrous. 13 figs.

  8. Dynamics and control of DNA sequence amplification

    SciTech Connect (OSTI)

    Marimuthu, Karthikeyan [Department of Chemical Engineering and Center for Advanced Process Decision-Making, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213 (United States); Chakrabarti, Raj, E-mail: raj@pmc-group.com, E-mail: rajc@andrew.cmu.edu [Department of Chemical Engineering and Center for Advanced Process Decision-Making, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213 (United States); Division of Fundamental Research, PMC Advanced Technology, Mount Laurel, New Jersey 08054 (United States)

    2014-10-28T23:59:59.000Z

    DNA amplification is the process of replication of a specified DNA sequence in vitro through time-dependent manipulation of its external environment. A theoretical framework for determination of the optimal dynamic operating conditions of DNA amplification reactions, for any specified amplification objective, is presented based on first-principles biophysical modeling and control theory. Amplification of DNA is formulated as a problem in control theory with optimal solutions that can differ considerably from strategies typically used in practice. Using the Polymerase Chain Reaction as an example, sequence-dependent biophysical models for DNA amplification are cast as control systems, wherein the dynamics of the reaction are controlled by a manipulated input variable. Using these control systems, we demonstrate that there exists an optimal temperature cycling strategy for geometric amplification of any DNA sequence and formulate optimal control problems that can be used to derive the optimal temperature profile. Strategies for the optimal synthesis of the DNA amplification control trajectory are proposed. Analogous methods can be used to formulate control problems for more advanced amplification objectives corresponding to the design of new types of DNA amplification reactions.

  9. DNA Motif Representation with Nucleotide Dependency

    E-Print Network [OSTI]

    Chin, Francis Y.L.

    DNA Motif Representation with Nucleotide Dependency Francis Chin1 and Henry Leung1 1 Department, these representations cannot model biological binding sites well because they fail to capture nucleotide interdependence. It has been pointed out by many researchers that the nucleotides of the DNA binding site cannot

  10. Atomic force microscopy of biochemically tagged DNA

    SciTech Connect (OSTI)

    Ogletree, D.F.; Kolbe, W.; Spengler, S.; Salmeron, M. (Lawrence Berkeley Laboratory, CA (United States)); Hansma, H.G.; Bezanilla, M. (Univ. of California, Santa Barbara (United States)); Sano, T.; Smith, C.S.; Cantor, C.R. (Boston Univ., MA (United States))

    1993-05-01T23:59:59.000Z

    Small fragments of DNA of known length were made with the polymerase chain reaction. These fragments had biotin molecules covalently attached at their ends. They were subsequently labeled with a chimeric protein fusion between streptavidin and two immunoglobulin G-binding domains of staphyloccocal protein A. This tetrameric species was expected to bind up to four DNA molecules via their attached biotin moieties. The DNA-protein complex was deposited on mica and imaged with an atomic force microscope. The images revealed the protein chimera at the expected location at the ends of the strands of DNA as well as the expected dimers, trimers, and tetramers of DNA bound to a single protein. 25 refs., 5 figs.

  11. Storing data encoded DNA in living organisms

    DOE Patents [OSTI]

    Wong; Pak C. (Richland, WA), Wong; Kwong K. (Sugar Land, TX), Foote; Harlan P. (Richland, WA)

    2006-06-06T23:59:59.000Z

    Current technologies allow the generation of artificial DNA molecules and/or the ability to alter the DNA sequences of existing DNA molecules. With a careful coding scheme and arrangement, it is possible to encode important information as an artificial DNA strand and store it in a living host safely and permanently. This inventive technology can be used to identify origins and protect R&D investments. It can also be used in environmental research to track generations of organisms and observe the ecological impact of pollutants. Today, there are microorganisms that can survive under extreme conditions. As well, it is advantageous to consider multicellular organisms as hosts for stored information. These living organisms can provide as memory housing and protection for stored data or information. The present invention provides well for data storage in a living organism wherein at least one DNA sequence is encoded to represent data and incorporated into a living organism.

  12. Elastic and Proton Dynamics of the DNA

    E-Print Network [OSTI]

    V. L. Golo

    2008-03-28T23:59:59.000Z

    The subject of this report is the dynamics of elastic system in conjunction with hydrogen bonds of the DNA. We draw attention to the draw-back of the familiar rod model of the DNA, and make a case of constructing models that could accommodate the intrinsic structure of the DNA. In this respect studying the interplay among the elastic system and the protons of the DNA, is of interest, for it could accommodate the inter-strand as well as the tunneling modes of protons. Following this direction, we come to the conclusion that the elastic-proton dynamics may have a bearing on biophysics of the DNA. The phenomenon of point mutations is discussed within this framework.

  13. Flow cytometric detection method for DNA samples

    DOE Patents [OSTI]

    Nasarabadi,Shanavaz (Livermore, CA); Langlois, Richard G. (Livermore, CA); Venkateswaran, Kodumudi S. (Round Rock, TX)

    2011-07-05T23:59:59.000Z

    Disclosed herein are two methods for rapid multiplex analysis to determine the presence and identity of target DNA sequences within a DNA sample. Both methods use reporting DNA sequences, e.g., modified conventional Taqman.RTM. probes, to combine multiplex PCR amplification with microsphere-based hybridization using flow cytometry means of detection. Real-time PCR detection can also be incorporated. The first method uses a cyanine dye, such as, Cy3.TM., as the reporter linked to the 5' end of a reporting DNA sequence. The second method positions a reporter dye, e.g., FAM.TM. on the 3' end of the reporting DNA sequence and a quencher dye, e.g., TAMRA.TM., on the 5' end.

  14. Flow cytometric detection method for DNA samples

    DOE Patents [OSTI]

    Nasarabadi, Shanavaz (Livermore, CA); Langlois, Richard G. (Livermore, CA); Venkateswaran, Kodumudi S. (Livermore, CA)

    2006-08-01T23:59:59.000Z

    Disclosed herein are two methods for rapid multiplex analysis to determine the presence and identity of target DNA sequences within a DNA sample. Both methods use reporting DNA sequences, e.g., modified conventional Taqman.RTM. probes, to combine multiplex PCR amplification with microsphere-based hybridization using flow cytometry means of detection. Real-time PCR detection can also be incorporated. The first method uses a cyanine dye, such as, Cy3.TM., as the reporter linked to the 5' end of a reporting DNA sequence. The second method positions a reporter dye, e.g., FAM, on the 3' end of the reporting DNA sequence and a quencher dye, e.g., TAMRA, on the 5' end.

  15. DNA tethering characterization, enzyme-mediated DNA looping under tension, and nucleosome stability in the force measuring optical tweezers

    E-Print Network [OSTI]

    Gemmen, Gregory John

    2006-01-01T23:59:59.000Z

    M. & Schiessel, H. (2004). DNA spools under tension. Phys.accessibility of nucleosomal DNA. Nat. Struct. Mol. Biol.elasticity of lambda-phage DNA. Science . 265 : 15991600.

  16. DNA Damage, Repair & Replication Using E. Coli Model Systems

    E-Print Network [OSTI]

    Troll, Christopher

    2013-01-01T23:59:59.000Z

    from DNA containing dIMP residues by the Escherichia coli,from DNA containing dIMP residues by the Escherichia coli,

  17. Constraint of DNA on Functionalized Graphene Improves Its Biostability...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Constraint of DNA on Functionalized Graphene Improves Its Biostability and Specificity. Constraint of DNA on Functionalized Graphene Improves Its Biostability and Specificity....

  18. ap dna endonuclease: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    specificity Raines, Ronald T. 2 A DNA and restriction enzyme implementation of Turing Ma (Turing machines; Universal Turing machines; recombinant DNA; nonpalindromic...

  19. adenovirus dna sequences: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    description of the given DNA string in terms of a smaller set of distinct domain labels. This yields a minimal domain description of a given DNA sequence, significantly...

  20. andribosomal dna sequences: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    description of the given DNA string in terms of a smaller set of distinct domain labels. This yields a minimal domain description of a given DNA sequence, significantly...

  1. IN VITRO MUTAGENIC AND DNA AND CHROMOSOMAL DAMAGE ACTIVITY BY...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    IN VITRO MUTAGENIC AND DNA AND CHROMOSOMAL DAMAGE ACTIVITY BY SURFACTANT DISPERSION OR SOLVENT EXTRACT OF A REFERENCE DIESEL EXHAUST PARTICULATE MATERIAL IN VITRO MUTAGENIC AND DNA...

  2. Electrochemical Branched-DNA Assay for Polymerase Chain Reaction...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Branched-DNA Assay for Polymerase Chain Reaction-Free Detection and Quantification of Oncogenes in Messenger RNA Electrochemical Branched-DNA Assay for Polymerase Chain...

  3. adapting dna microarray: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Gene Expression Analysis & R Tutorial 12;DNA Microarrays An R Tutorial Functional Genomics Qiu, Weigang 3 Research tools known as DNA microarrays are Biology and Medicine...

  4. archived dna microarrays: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Gene Expression Analysis & R Tutorial 12;DNA Microarrays An R Tutorial Functional Genomics Qiu, Weigang 3 Research tools known as DNA microarrays are Biology and Medicine...

  5. Protein Bridges DNA Base and Nucleotide Excision Repair Pathways

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    a nucleotide from the DNA double helix to its active site to access damaged nucleotides. But unlike AGT and most other known DNA nucleotide-flipping proteins, this...

  6. DNA Replication via Entanglement Swapping

    E-Print Network [OSTI]

    Onur Pusuluk; Cemsinan Deliduman

    2011-03-31T23:59:59.000Z

    Quantum effects are mainly used for the determination of molecular shapes in molecular biology, but quantum information theory may be a more useful tool to understand the physics of life. Organic molecules and quantum circuits/protocols can be considered as hardware and software of living systems that are co-optimized during evolution. We try to model DNA replication in this sense as a multi-body entanglement swapping with a reliable qubit representation of the nucleotides. In our model molecular recognition of a nucleotide triggers an intrabase entanglement corresponding to a superposition state of different tautomer forms. Then, base pairing occurs by swapping intrabase entanglements with interbase entanglements. We examine possible realizations of quantum circuits to be used to obtain intrabase entanglement and swapping protocols to be employed to obtain interbase entanglement. Finally, we discuss possible ways for computational and experimental verification of the model.

  7. Abstract DNA-type systems

    E-Print Network [OSTI]

    Diederik Aerts; Marek Czachor

    2005-12-22T23:59:59.000Z

    An abstract DNA-type system is defined by a set of nonlinear kinetic equations with polynomial nonlinearities that admit soliton solutions associated with helical geometry. The set of equations allows for two different Lax representations: A von Neumann form and a Darboux-covariant Lax pair. We explain why non-Kolmogorovian probability models occurring in soliton kinetics are naturally associated with chemical reactions. The most general known characterization of soliton kinetic equations is given and a class of explicit soliton solutions is discussed. Switching between open and closed states is a generic behaviour of the helices. The effect does not crucially depend on the order of nonlinearity (i.e. types of reactions), a fact that may explain why simplified models possess properties occuring in realistic systems. We explain also why fluctuations based on Darboux transformations will not destroy the dynamics but only switch between a finite number of helical structures.

  8. Acidic C-terminal tail of the ssDNA-binding protein of bacteriophage T7 and ssDNA

    E-Print Network [OSTI]

    Richardson, Charles C.

    Acidic C-terminal tail of the ssDNA-binding protein of bacteriophage T7 and ssDNA compete. Prokaryotic ssDNA-binding proteins share a conserved DNA-binding fold and an acidic C-terminal tail. It has been proposed that in the absence of ssDNA, the C-terminal tail contacts the ssDNA-binding cleft

  9. DNA fragment sizing and sorting by laser-induced fluorescence

    DOE Patents [OSTI]

    Hammond, Mark L. (Angier, NC); Jett, James H. (Los Alamos, NM); Keller, Richard A. (Los Alamos, NM); Marrone, Babetta L. (Los Alamos, NM); Martin, John C. (Los Alamos, NM)

    1996-01-01T23:59:59.000Z

    A method is provided for sizing DNA fragments using high speed detection systems, such as flow cytometry to determine unique characteristics of DNA pieces from a sample. In one characterization the DNA piece is fragmented at preselected sites to produce a plurality of DNA fragments. The DNA piece or the resulting DNA fragments are treated with a dye effective to stain stoichiometrically the DNA piece or the DNA fragments. The fluorescence from the dye in the stained fragments is then examined to generate an output functionally related to the number of nucleotides in each one of the DNA fragments. In one embodiment, the intensity of the fluorescence emissions from each fragment is linearly related to the fragment length. The distribution of DNA fragment sizes forms a characterization of the DNA piece for use in forensic and research applications.

  10. Method of quantitating dsDNA

    DOE Patents [OSTI]

    Stark, Peter C. (Los Alamos, NM); Kuske, Cheryl R. (Los Alamos, NM); Mullen, Kenneth I. (Los Alamos, NM)

    2002-01-01T23:59:59.000Z

    A method for quantitating dsDNA in an aqueous sample solution containing an unknown amount of dsDNA. A first aqueous test solution containing a known amount of a fluorescent dye-dsDNA complex and at least one fluorescence-attenutating contaminant is prepared. The fluorescence intensity of the test solution is measured. The first test solution is diluted by a known amount to provide a second test solution having a known concentration of dsDNA. The fluorescence intensity of the second test solution is measured. Additional diluted test solutions are similarly prepared until a sufficiently dilute test solution having a known amount of dsDNA is prepared that has a fluorescence intensity that is not attenuated upon further dilution. The value of the maximum absorbance of this solution between 200-900 nanometers (nm), referred to herein as the threshold absorbance, is measured. A sample solution having an unknown amount of dsDNA and an absorbance identical to that of the sufficiently dilute test solution at the same chosen wavelength is prepared. Dye is then added to the sample solution to form the fluorescent dye-dsDNA-complex, after which the fluorescence intensity of the sample solution is measured and the quantity of dsDNA in the sample solution is determined. Once the threshold absorbance of a sample solution obtained from a particular environment has been determined, any similarly prepared sample solution taken from a similar environment and having the same value for the threshold absorbance can be quantified for dsDNA by adding a large excess of dye to the sample solution and measuring its fluorescence intensity.

  11. , btzhang}@bi.snu.ac.kr Boosted DNA Computing for Evolutionary Graphical Structure Learning

    E-Print Network [OSTI]

    PLM (Probabilistic Library Model PLM PLM DNA bead seperation PCR (Polymerase Chain Reaction) PLM PLM DNA DNA PLM PLM PLM PLM DNA PLM PLM evolutionary graph structure PLM DNA &$$) Jc`" '& Bc" %6 #12

  12. DNA looping: the consequences and its control

    E-Print Network [OSTI]

    Leonor Saiz; Jose M. G. Vilar

    2006-09-26T23:59:59.000Z

    The formation of DNA loops by proteins and protein complexes is ubiquitous to many fundamental cellular processes, including transcription, recombination, and replication. Here we review recent advances in understanding the properties of DNA looping in its natural context and how they propagate to the cellular behavior through gene regulation. The results of connecting the molecular properties with cellular physiology indicate that looping of DNA in vivo is much more complex and easier than predicted from current models and reveals a wealth of previously unappreciated details.

  13. DNA adsorption at liquid/solid interfaces

    E-Print Network [OSTI]

    Carine Douarche; Robert Corts; Steven J. Roser; Jean-Louis Sikorav; Alan Braslau

    2008-09-26T23:59:59.000Z

    DNA adsorption on solid or liquid surfaces is a topic of broad fundamental and applied interest. Here we study by x-ray reflectivity the adsorption of monodisperse double-stranded DNA molecules a positively-charged surface, obtained through chemical grafting of a homogeneous organicmonomolecular layer of N-(2-aminoethyl) dodecanamide on an oxide-free monocrystalline Si(111) wafer. The adsorbed dsDNA is found to embed into the soft monolayer which is deformed in the process. The surface coverage is very high and this adsorbed layer is expected to display 2D nematic ordering.

  14. Sequential addition of short DNA oligos in DNA-polymerase-based synthesis reactions

    DOE Patents [OSTI]

    Gardner, Shea N; Mariella, Jr., Raymond P; Christian, Allen T; Young, Jennifer A; Clague, David S

    2013-06-25T23:59:59.000Z

    A method of preselecting a multiplicity of DNA sequence segments that will comprise the DNA molecule of user-defined sequence, separating the DNA sequence segments temporally, and combining the multiplicity of DNA sequence segments with at least one polymerase enzyme wherein the multiplicity of DNA sequence segments join to produce the DNA molecule of user-defined sequence. Sequence segments may be of length n, where n is an odd integer. In one embodiment the length of desired hybridizing overlap is specified by the user and the sequences and the protocol for combining them are guided by computational (bioinformatics) predictions. In one embodiment sequence segments are combined from multiple reading frames to span the same region of a sequence, so that multiple desired hybridizations may occur with different overlap lengths.

  15. The unholy trinity: taxonomy, species delimitation and DNA barcoding

    E-Print Network [OSTI]

    DeSalle, Rob

    The unholy trinity: taxonomy, species delimitation and DNA barcoding Rob DeSalle*, Mary G. Egan are clarified and resolved, before the use of DNA as a tool for taxonomy and species delimitation can framework for interweaving classical taxonomy with the goals of `DNA barcoding'. Keywords: DNA barcoding

  16. DNA ARRAY DECODING FROM NONLINEAR MEASUREMENTS BY BELIEF PROPAGATION

    E-Print Network [OSTI]

    DNA ARRAY DECODING FROM NONLINEAR MEASUREMENTS BY BELIEF PROPAGATION Mona A. Sheikh, Shriram Compressed Sensing (CS) and demonstrate its utility in DNA array decoding. In a CS DNA microarray, the array spots identify DNA sequences that are shared between multiple organisms, thereby reduc- ing the number

  17. DNA Oligonucleotide Synthesis in Mesoporous Silicon for Biosensing Applications

    E-Print Network [OSTI]

    Weiss, Sharon

    DNA Oligonucleotide Synthesis in Mesoporous Silicon for Biosensing Applications Jenifer L. Lawrie for improving the sensitivity of label-free optical biosensors based on in-situ synthesis of DNA probes within was utilized as the sensor structure. Synthesis of DNA probe, as well as sensing of target DNA, was verified

  18. DNA Deformation Energy as an Indirect Recognition Mechanism in

    E-Print Network [OSTI]

    Lathrop, Richard H.

    DNA Deformation Energy as an Indirect Recognition Mechanism in Protein-DNA Interactions Kimberly A. Senear Abstract--Proteins that bind to specific locations in genomic DNA control many basic cellular. Deformation energy, which models the energy required to bend DNA from its native shape to its shape when bound

  19. DNA Translocation Governed by Interactions with Solid-State Nanopores

    E-Print Network [OSTI]

    Meller, Amit

    DNA Translocation Governed by Interactions with Solid-State Nanopores Meni Wanunu, Jason Sutin, Ben dynamics of individual DNA molecules through solid-state nanopores in the diameter range 2.75 nm. Our with DNA length by two power laws: for short DNA molecules, in the range 1503500 bp, we find an exponent

  20. DNA Compression Challenge Revisited: a Dynamic Programming Approach

    E-Print Network [OSTI]

    Lonardi, Stefano

    DNA Compression Challenge Revisited: a Dynamic Programming Approach Behshad Behzadi and Fabrice Le Fessant LIX, Ecole Polytechnique, Paris, FRANCE June 21 2005 B. Behzadi, F. Le Fessant (LIX) DNA Compression June 21 2005 1 / 38 #12;Outline 1 DNA Compression Challenge 2 Tools and Methods 3 DNA Compression

  1. DNA Computing: A Research University of Western Ontario

    E-Print Network [OSTI]

    Kari, Lila

    31 DNA Computing: A Research Snapshot Lila Kari University of Western Ontario Kalpana Mahalingam ........................................... 31-6 31.5 DNA Memory ................................................... 31-8 Nested Primer Molecular Memory Organic DNA Memory Design of DNA Sequences 31.6 Computation in Living Cells

  2. Deoxyribose oxidation chemistry and endogenous DNA adducts

    E-Print Network [OSTI]

    Zhou, Xinfeng

    2006-01-01T23:59:59.000Z

    Endogenous and exogenous oxidants react with cellular macromolecules to generate a variety of electrophiles that react with DNA produce cytotoxic and mutagenic adducts. One source of such electrophiles is deoxyribose in ...

  3. DNA Assembly Line for Nano-Construction

    ScienceCinema (OSTI)

    Oleg Gang

    2010-01-08T23:59:59.000Z

    Building on the idea of using DNA to link up nanoparticles scientists at Brookhaven National Lab have designed a molecular assembly line for high-precision nano-construction. Nanofabrication is essential for exploiting the unique properties of nanoparticl

  4. Linear Thermodynamics of Rodlike DNA Filtration

    E-Print Network [OSTI]

    Li, Zirui

    Linear thermodynamics transportation theory is employed to study filtration of rodlike DNA molecules. Using the repeated nanoarray consisting of alternate deep and shallow regions, it is demonstrated that the complex ...

  5. Intriguing DNA Editor Has a Structural Trigger

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Intriguing DNA Editor Has a Structural Trigger Print A powerful new tool for genome editing and gene regulation has emerged in the form of a family of enzymes known as Cas9. Cas9...

  6. A model for melting of confined DNA

    E-Print Network [OSTI]

    Werner, E; Ambjrnsson, T; Mehlig, B

    2015-01-01T23:59:59.000Z

    When DNA molecules are heated they denature. This occurs locally so that loops of molten single DNA strands form, connected by intact double-stranded DNA pieces. The properties of this "melting" transition have been intensively investigated. Recently there has been a surge of interest in this question, caused by experiments determining the properties of partially bound DNA confined to nanochannels. But how does such confinement affect the melting transition? To answer this question we introduce, and solve a model predicting how confinement affects the melting transition for a simple model system by first disregarding the effect of self-avoidance. We find that the transition is smoother for narrower channels. By means of Monte-Carlo simulations we then show that a model incorporating self-avoidance shows qualitatively the same behaviour and that the effect of confinement is stronger than in the ideal case.

  7. The Initiation of Bacterial DNA Replication

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    that the ATP-DnaA active site is in a closed configuration, which allows it to bind nucleotides using conserved residues from neighboring AAA+ protomers. The precise geometry of...

  8. RecA acts in trans to allow replication of damaged DNA by DNA polymerase V

    E-Print Network [OSTI]

    Cox, Michael M.

    , Michael M. Cox2 , Roger Woodgate3 & Myron F. Goodman1 The DNA polymerase V (pol V) and RecA proteins

  9. Carbon Nanotube DNA Sensor and Sensing Mechanism

    E-Print Network [OSTI]

    Le Roy, Robert J.

    nanotube (SWNT) DNA sensors and the sensing mechanism. The simple and generic protocol for label for direct label-free detection of DNA hybridization in a biocompatible buffer solution. We also carried out is a field effect device, which has a typical on-current of 3-6 A at 10 mV source- drain bias and an on-off

  10. Regulation of DNA repair by parkin

    SciTech Connect (OSTI)

    Kao, Shyan-Yuan, E-mail: shyan-yuan_kao@meei.harvard.edu [Eaton Peabody Laboratory, Massachusetts Eye and Ear Infirmary, 243 Charles Street, Boston, MA 02114 (United States)] [Eaton Peabody Laboratory, Massachusetts Eye and Ear Infirmary, 243 Charles Street, Boston, MA 02114 (United States)

    2009-05-01T23:59:59.000Z

    Mutation of parkin is one of the most prevalent causes of autosomal recessive Parkinson's disease (PD). Parkin is an E3 ubiquitin ligase that acts on a variety of substrates, resulting in polyubiquitination and degradation by the proteasome or monoubiquitination and regulation of biological activity. However, the cellular functions of parkin that relate to its pathological involvement in PD are not well understood. Here we show that parkin is essential for optimal repair of DNA damage. Parkin-deficient cells exhibit reduced DNA excision repair that can be restored by transfection of wild-type parkin, but not by transfection of a pathological parkin mutant. Parkin also protects against DNA damage-induced cell death, an activity that is largely lost in the pathological mutant. Moreover, parkin interacts with the proliferating cell nuclear antigen (PCNA), a protein that coordinates DNA excision repair. These results suggest that parkin promotes DNA repair and protects against genotoxicity, and implicate DNA damage as a potential pathogenic mechanism in PD.

  11. DNA Polymerase-Mediated DNA Synthesis on a TNA Template John C. Chaput, Justin K. Ichida, and Jack W. Szostak*

    E-Print Network [OSTI]

    Heller, Eric

    DNA Polymerase-Mediated DNA Synthesis on a TNA Template John C. Chaput, Justin K. Ichida, and Jack TNA is capable of antiparallel, Watson-Crick base-pairing with complementary DNA, RNA, and TNA oligo- nucleotides.2 This property is remarkable, given that the TNA repeat unit is one atom shorter than that of DNA

  12. Optical selection and collection of DNA fragments

    DOE Patents [OSTI]

    Roslaniec, Mary C. (Los Alamos, NM); Martin, John C. (Los Alamos, NM); Jett, James H. (Los Alamos, NM); Cram, L. Scott (Los Alamos, NM)

    1998-01-01T23:59:59.000Z

    Optical selection and collection of DNA fragments. The present invention includes the optical selection and collection of large (>.mu.g) quantities of clonable, chromosome-specific DNA from a sample of chromosomes. Chromosome selection is based on selective, irreversible photoinactivation of unwanted chromosomal DNA. Although more general procedures may be envisioned, the invention is demonstrated by processing chromosomes in a conventional flow cytometry apparatus, but where no droplets are generated. All chromosomes in the sample are first stained with at least one fluorescent analytic dye and bonded to a photochemically active species which can render chromosomal DNA unclonable if activated. After passing through analyzing light beam(s), unwanted chromosomes are irradiated using light which is absorbed by the photochemically active species, thereby causing photoinactivation. As desired chromosomes pass this photoinactivation point, the inactivating light source is deflected by an optical modulator; hence, desired chromosomes are not photoinactivated and remain clonable. The selection and photoinactivation processes take place on a microsecond timescale. By eliminating droplet formation, chromosome selection rates 50 times greater than those possible with conventional chromosome sorters may be obtained. Thus, usable quantities of clonable DNA from any source thereof may be collected.

  13. DNA nanotechnology: understanding and optimisation through simulation

    E-Print Network [OSTI]

    Thomas E. Ouldridge

    2014-11-07T23:59:59.000Z

    DNA nanotechnology promises to provide controllable self-assembly on the nanoscale, allowing for the design of static structures, dynamic machines and computational architectures. In this article I review the state-of-the art of DNA nanotechnology, highlighting the need for a more detailed understanding of the key processes, both in terms of theoretical modelling and experimental characterisation. I then consider coarse-grained models of DNA, mesoscale descriptions that have the potential to provide great insight into the operation of DNA nanotechnology if they are well designed. In particular, I discuss a number of nanotechnological systems that have been studied with oxDNA, a recently developed coarse-grained model, highlighting the subtle interplay of kinetic, thermodynamic and mechanical factors that can determine behaviour. Finally, new results highlighting the importance of mechanical tension in the operation of a two-footed walker are presented, demonstrating that recovery from an unintended `overstepped' configuration can be accelerated by three to four orders of magnitude by application of a moderate tension to the walker's track. More generally, the walker illustrates the possibility of biasing strand-displacement processes to affect the overall rate.

  14. Structural Studies of E. coli Topoisomerase III-DNA Complexes Reveal a Novel Type IA Topoisomerase-DNA Conformational Intermediate

    SciTech Connect (OSTI)

    Changela, Anita; DiGate, Russell J.; Mondragon, Alfonso (NWU); (Phil. Col. Pharmacy)

    2010-03-05T23:59:59.000Z

    Escherichia coli DNA topoisomerase III belongs to the type IA family of DNA topoisomerases, which transiently cleave single-stranded DNA (ssDNA) via a 5{prime} phosphotyrosine intermediate. We have solved crystal structures of wild-type E. coli topoisomerase III bound to an eight-base ssDNA molecule in three different pH environments. The structures reveal the enzyme in three distinct conformational states while bound to DNA. One conformation resembles the one observed previously with a DNA-bound, catalytically inactive mutant of topoisomerase III where DNA binding realigns catalytic residues to form a functional active site. Another conformation represents a novel intermediate in which DNA is bound along the ssDNA-binding groove but does not enter the active site, which remains in a catalytically inactive, closed state. A third conformation shows an intermediate state where the enzyme is still in a closed state, but the ssDNA is starting to invade the active site. For the first time, the active site region in the presence of both the catalytic tyrosine and ssDNA substrate is revealed for a type IA DNA topoisomerase, although there is no evidence of ssDNA cleavage. Comparative analysis of the various conformational states suggests a sequence of domain movements undertaken by the enzyme upon substrate binding.

  15. Conditions for positioning of nucleosomes on DNA

    E-Print Network [OSTI]

    Michael Sheinman; Ho-Ryun Chung

    2015-04-29T23:59:59.000Z

    Positioning of nucleosomes along eukaryotic genomes plays an important role in their organization and regulation. There are many different factors affecting the location of nucleosomes. Some can be viewed as preferential binding of a single nucleosome to different locations along the DNA and some as interactions between neighboring nucleosomes. In this study we analyzed how well nucleosomes are positioned along the DNA as a function of strength of the preferential binding, correlation length of the binding energy landscape, interactions between neighboring nucleosomes and others relevant system properties. We analyze different scenarios: designed energy landscapes and generically disordered ones and derive conditions for good positioning. Using analytic and numerical approaches we find that, even if the binding preferences are very weak, synergistic interplay between the interactions and the binding preferences is essential for a good positioning of nucleosomes, especially on correlated energy landscapes. Analyzing empirical energy landscape, we discuss relevance of our theoretical results to positioning of nucleosomes on DNA \\emph{in vivo.}

  16. Sequential addition of short DNA oligos in DNA-polymerase-based synthesis reactions

    DOE Patents [OSTI]

    Gardner, Shea N. (San Leandro, CA); Mariella, Jr., Raymond P. (Danville, CA); Christian, Allen T. (Tracy, CA); Young, Jennifer A. (Berkeley, CA); Clague, David S. (Livermore, CA)

    2011-01-18T23:59:59.000Z

    A method of fabricating a DNA molecule of user-defined sequence. The method comprises the steps of preselecting a multiplicity of DNA sequence segments that will comprise the DNA molecule of user-defined sequence, separating the DNA sequence segments temporally, and combining the multiplicity of DNA sequence segments with at least one polymerase enzyme wherein the multiplicity of DNA sequence segments join to produce the DNA molecule of user-defined sequence. Sequence segments may be of length n, where n is an even or odd integer. In one embodiment the length of desired hybridizing overlap is specified by the user and the sequences and the protocol for combining them are guided by computational (bioinformatics) predictions. In one embodiment sequence segments are combined from multiple reading frames to span the same region of a sequence, so that multiple desired hybridizations may occur with different overlap lengths. In one embodiment starting sequence fragments are of different lengths, n, n+1, n+2, etc.

  17. Dynamic cluster-scaling in DNA

    E-Print Network [OSTI]

    A. Bershadskii

    2010-08-07T23:59:59.000Z

    It is shown that the nucleotide sequences in DNA molecules have cluster-scaling properties (discovered for the first time in turbulent processes: Sreenivasan and Bershadskii, 2006, J. Stat. Phys., 125, 1141-1153.). These properties are relevant to both types of nucleotide pair-bases interactions: hydrogen bonds and stacking interactions. It is shown that taking into account the cluster-scaling properties can help to improve heterogeneous models of the DNA dynamics. Two human genes: BRCA2 and NRXN1, have been considered as examples.

  18. Ubiquitylation, neddylation and the DNA damage response

    E-Print Network [OSTI]

    Brown, Jessica S.; Jackson, Stephen P.

    2015-04-01T23:59:59.000Z

    major families: RING (really interesting new gene), HECT (homology to E6AP car- boxyl-terminus) and RBR (ring between ring) [102,103]. The transcription, respectively [88] trans-lesion synthesis (TLS) TLS is a DNA damage bypass mechanism tha It employs... depends on RNF4 binding to SUMO2/3 polymeric chains and subsequent RNF4 dimerization [183]. In addition to its role in promoting the turnover of proteins, RNF4 might also be important for the formation of hybrid SUMO/ubiquitin chains at DNA damage sites...

  19. Inhibition of DNA Supercoiling-dependent Transcriptional Activation by a Distant B-DNA to Z-DNA Transition*

    E-Print Network [OSTI]

    Benham, Craig J.

    that a supercoiling-dependent, DNA structural transmission mechanism of this type is responsible for the integration over other alterna- tives, primarily because the change from right-handed helix to left-handed helix). In this way one can alter the destabilization characteristics of other local regions without changing

  20. Studies of DNA dynamics in slit-like nanochannel confinement

    E-Print Network [OSTI]

    Balducci, Anthony (Anthony G.)

    2008-01-01T23:59:59.000Z

    The ability to visually observe single DNA molecules has greatly improved our understanding of polymer physics, from gel electrophoresis to the theology of dilute (and even concentrated) polymer solutions. The use of DNA ...

  1. DNA hybridization : fundamental studies and applications in directed assembly

    E-Print Network [OSTI]

    Bajaj, Manish G. (Manish Gopal)

    2005-01-01T23:59:59.000Z

    Programmed self-assembly using non-covalent DNA-DNA interactions is a promising technique for the creation of next-generation functional devices for electronic, optical, and magnetic applications. This thesis develops the ...

  2. Genome scanning : an AFM-based DNA sequencing technique

    E-Print Network [OSTI]

    Elmouelhi, Ahmed (Ahmed M.), 1979-

    2003-01-01T23:59:59.000Z

    Genome Scanning is a powerful new technique for DNA sequencing. The method presented in this thesis uses an atomic force microscope with a functionalized cantilever tip to sequence single stranded DNA immobilized to a mica ...

  3. Protein-DNA interaction, random walks and polymer statistics

    E-Print Network [OSTI]

    Slutsky, Michael

    2005-01-01T23:59:59.000Z

    In Part I of the thesis, a general physical framework describing the kinetics of protein- DNA interaction is developed. Recognition and binding of specific sites on DNA by proteins is central for many cellular functions ...

  4. Role of DNA repair protein ERCC1 in skin cancer

    E-Print Network [OSTI]

    Song, Liang

    2009-01-01T23:59:59.000Z

    Nucleotide excision repair (NER) is one of the major repair systems for removal of DNA lesions. The NER pathway has evolved mainly to repair UV-induced DNA damage and is also active against a broad range of endogenously ...

  5. Mitigating security issues in the evolving DNA synthesis industry

    E-Print Network [OSTI]

    Turlington, Ralph Donald, III

    2013-01-01T23:59:59.000Z

    DNA synthesis technologies are advancing at exponential rates, with production of ever longer, more complex, and less expensive sequences of double stranded DNA. This has fostered development of industrial scale design, ...

  6. Defining functional DNA elements in the human genome

    E-Print Network [OSTI]

    Kellis, Manolis

    With the completion of the human genome sequence, attention turned to identifying and annotating its functional DNA elements. As a complement to genetic and comparative genomics approaches, the Encyclopedia of DNA Elements ...

  7. Lectin cDNA and transgenic plants derived therefrom

    DOE Patents [OSTI]

    Raikhel, Natasha V. (Okemos, MI)

    2000-10-03T23:59:59.000Z

    Transgenic plants containing cDNA encoding Gramineae lectin are described. The plants preferably contain cDNA coding for barley lectin and store the lectin in the leaves. The transgenic plants, particularly the leaves exhibit insecticidal and fungicidal properties.

  8. A model for sample stacking in microcapillary DNA electrophoresis

    E-Print Network [OSTI]

    Srivastava, Alok Kumar, 1967-

    2002-01-01T23:59:59.000Z

    Sanger's method of chain termination is the method of choice in DNA sequencing, where electrophoresis is used to separate the different sized DNA. In the past decade, microfabricated capillary devices have been developed ...

  9. Local alignment of generalized k-base encoded DNA sequence

    E-Print Network [OSTI]

    Homer, Nils; Nelson, Stanley F; Merriman, Barry

    2010-01-01T23:59:59.000Z

    k-base encoded DNA sequence BMC Bioinformatics 2010, 11:347similarities in the amino acid sequence of two proteins. Jof two-base encoded DNA sequence. BMC Bioinformatics 2009,

  10. Flow cytometric measurement of total DNA and incorporated halodeoxyuridine

    DOE Patents [OSTI]

    Dolbeare, F.A.; Gray, J.W.

    1983-10-18T23:59:59.000Z

    A method for the simultaneous flow cylometric measurement of total cellular DNA content and of the uptake of DNA precursors as a measure of DNA synthesis during various phases of the cell cycle in normal and malignant cells in vitro and in vivo is described. The method comprises reacting cells with labelled halodeoxyuridine (HdU), partially denaturing cellular DNA, adding to the reaction medium monoclonal antibodies (mabs) reactive with HdU, reacting the bound mabs with a second labelled antibody, incubating the mixture with a DNA stain, and measuring simultaneously the intensity of the DNA stain as a measure of the total cellular DNA and the HdU incorporated as a measure of DNA synthesis. (ACR)

  11. Photoelectrochemical array platform for genomic scale DNA synthesis

    E-Print Network [OSTI]

    Emig, Christopher Joseph

    2006-01-01T23:59:59.000Z

    Molecular and synthetic biologists have increasing demand for large, high-fidelity constructs of synthetic DNA. Recent developments in harvesting oligonucleotides from DNA microarrays has proven that these can be assembled ...

  12. Enhancement of in vitro Translation by Gold Nanoparticle DNA Conjugates

    E-Print Network [OSTI]

    Park, Sunho

    Gold nanoparticle (AuNP)?DNA conjugates can enhance in vitro translation of a protein. Enhancement occurs via a combination of nonspecific adsorption of translation-related molecules and the ribosome to the AuNP?DNA and ...

  13. An Investigation on Gel Electrophoresis with Quantum Dots End-labeled DNA

    E-Print Network [OSTI]

    Chen, Xiaojia

    2009-05-15T23:59:59.000Z

    explored manipulating DNA fragments by end labeling DNA molecules with quantum dot nanocrystals. The quantum dot-DNA conjugates can be further modified through binding interactions with biotinylated single-stranded DNA primers. Single molecule visualization...

  14. RhoJ Regulates Melanoma Chemoresistance by Suppressing Pathways that Sense DNA Damage

    E-Print Network [OSTI]

    Ho, Hsiang; Aruri, Jayavani; Kapadia, Rubina; Mehr, Hootan; White, Michael A.; Ganesan, Anand K.

    2012-01-01T23:59:59.000Z

    Pathways that Sense DNA Damage $watermark-text Hsiang Ho 1 ,16. Roos WP, Kaina B. DNA damage-induced apoptosis: FromDNA lesions to the DNA damage response and apoptosis. Cancer

  15. A User's Guide to the Encyclopedia of DNA Elements (ENCODE)

    E-Print Network [OSTI]

    Bernstein, Bradley E.

    The mission of the Encyclopedia of DNA Elements (ENCODE) Project is to enable the scientific and medical communities to

  16. Beyond DNA origami: the unfolding prospects of

    E-Print Network [OSTI]

    Walter, Nils G.

    for functional nanodevices. Not limited to nucleic acids, these nanodevices can incorporate other structural and functional materials, such as proteins and nanoparticles, making them broadly useful for current and futureOpinion Beyond DNA origami: the unfolding prospects of nucleic acid nanotechnology Nicole

  17. Rewritable Memory by Controllable Nanopatterning of DNA

    E-Print Network [OSTI]

    Pierce, Niles A.

    ABSTRACT Fabricating a nanostructure capable of reversibly patterning molecules is a fundamental goal within nanotechnology, underlying diverse processes such as information storage, scaffold functioning of the device as rewritable memory. The bit state of each address is controlled by specific DNA

  18. Reprogramming DNA Methylation in Bovine Cells by Knocking Down DNA Methyltransferase-1 with RNA Interference

    E-Print Network [OSTI]

    Stroud, Todd

    2010-01-20T23:59:59.000Z

    Embryos derived by somatic cell nuclear transfer (SCNT) produce few pregnancies that result in a live, healthy offspring. This has largely been attributed to the aberrant reprogramming of the somatic cell DNA used for ...

  19. DNA Strands Attached Inside Single Conical Nanopores: Ionic Pore Characteristics and Insight into DNA Biophysics

    E-Print Network [OSTI]

    Nguyen, Gael; Howorka, Stefan; Siwy, Zuzanna S.

    2011-01-01T23:59:59.000Z

    Kathawalla et al. 1989; Heng et al. 2005; Keyser et al.due to the electric ?eld (Heng et al. 2005; Randall et al.DNA analysis. Nanomedicine Heng JB, Aksimentiev A, Ho C,

  20. Structural basis for the inhibition of human alkyladenine DNA by 3,N4-ethenocytosine containing DNA

    E-Print Network [OSTI]

    Lingaraju, Gondichatnahalli M.

    Reactive oxygen and nitrogen species, generated by neutrophils and macrophages in chronically inflamed tissues, readily damage DNA, producing a variety of potentially genotoxic etheno base lesions; such inflammation-related ...

  1. DNA ruler : enhancing nanopore sizing resolution by multiple measurements on the same DNA molecule

    E-Print Network [OSTI]

    Sen, Yi-Heng

    2012-01-01T23:59:59.000Z

    Nanopores are versatile sensors for label-free detection of single molecules and particles that have attracted attention for applications such as DNA sequencing and nanoparticle analysis. Detection of single molecules or ...

  2. Updating SRM 2391c: PCR-Based DNA Profiling Standard

    E-Print Network [OSTI]

    profiling SRM 2391 SRM 2391a SRM 2391b SRM 2391c SRM 2390 DNA profiling 2391 series PCR-based DNA profiling 40 NMDEPARTMENT OF PUBLIC SAFETY 10 DNASI 40 SEROLOGICAL RESEARCH INSTITUTE 11 CHARLOTTE- DNA CRIME LAB 50 SOUTH CAROLINA LAW ENFORCEMENT 20 ILLUMINA INC CARROLL PARK 50 UWA INTERNATIONAL

  3. Design and Characterization of Programmable DNA Nanotubes Supporting Information

    E-Print Network [OSTI]

    Fygenson, Deborah Kuchnir

    . DNA sample preparation: Lyophilized HPLC- or PAGE- purified DNA oligonucleotides were purchased from Integrated DNA Technologies (Coralville, IA), resuspended in water, quanti- tated by UV absorbance at 260 nm immersion and 40X/0.75 NA air objectives. Blue light was filtered from a mer- cury arc lamp thro

  4. A Simple and Fast DNA Compressor Giovanni Manzini

    E-Print Network [OSTI]

    Manzini, Giovanni

    A Simple and Fast DNA Compressor Giovanni Manzini Marcella Rastero February 17, 2004 Abstract. For this reason most DNA compressors work by searching and encoding approximate repeats. We depart from to the best DNA compressors. Another important feature of our algorithm is its small space occupancy which

  5. DNA overwinds when stretched , Zev Bryant2,4

    E-Print Network [OSTI]

    Gore, Jeff

    DNA overwinds when stretched Jeff Gore1 , Zev Bryant2,4 , Marcelo Nollmann2 , Mai U. Le2 , Nicholas R. Cozzarelli2 & Carlos Bustamante14 DNA is often modelled as an isotropic rod14 , but its coupling between twisting and stretch- ing degrees of freedom. Simple physical intuition predicts that DNA

  6. The DNA binding activity of p53 displays reactiondiffusion kinetics

    E-Print Network [OSTI]

    Hinow, Peter

    The DNA binding activity of p53 displays reactiondiffusion kinetics 26th Southeastern 37240 The DNA binding activity of p53 displays reactiondiffusion kinetics p. 1/2 #12;Collaborators, Vanderbilt University Emmanuele DiBenedetto, PhD, Department of Mathematics, Vanderbilt University The DNA

  7. DNA methylation and the analysis of CpG Islands

    E-Print Network [OSTI]

    Czygrinow, Andrzej

    DNA methylation and the analysis of CpG Islands in genomes M. F. Wojciechowski MAT 351 25 March 2005 #12;#12;Nucleotides #12;Base pairing * * #12;DNA methylation In mammalian genomes, methylation residues represent a target for covalent modification of DNA Cytosine is one of two bases found commonly

  8. DNA Nanotechnology DOI: 10.1002/anie.201206389

    E-Print Network [OSTI]

    Hone, James

    DNA Nanotechnology DOI: 10.1002/anie.201206389 Assembly of Heterogeneous Functional Nanomaterials on DNA Origami Scaffolds** Risheng Wang,* Colin Nuckolls, and Shalom J. Wind* Hybrid nanomaterial systems;[1] selective growth;[4,9] and DNA-mediated assem- bly,[3,8] including the formation of 3D

  9. DNA IN THE UK By Erica Van Coverden

    E-Print Network [OSTI]

    Goodwin, Kelly D.

    DNA IN THE UK By Erica Van Coverden Dr. Kelly Goodwin, a microbiologist at AOML, finished up. Those proteins then would be sequenced and the amino acid sequence would be used to backtrack to the DNA sequence that codes for that protein. In another approach, total DNA was extracted, digested

  10. Methods to alter levels of a DNA repair protein

    DOE Patents [OSTI]

    Petrini, John H.; Morgan, William Francis; Maser, Richard Scott; Carney, James Patrick

    2006-10-17T23:59:59.000Z

    An isolated and purified DNA molecule encoding a DNA repair protein, p95, is provided, as is isolated and purified p95. Also provided are methods of detecting p95 and DNA encoding p95. The invention further provides p95 knock-out mice.

  11. Reaction Temperature Constraints in DNA Computing Russell Deaton

    E-Print Network [OSTI]

    Deaton, Russell J.

    Reaction Temperature Constraints in DNA Computing Russell Deaton The Department of Electrical the thermodynamics of DNA melt- ing, a technique is proposed to choose a re- action temperature for the DNA computa the melting temperature, Tm. The melting temperature is determined from curves of UV absorbance versus

  12. Recurrence time statistics: Versatile tools for genomic DNA sequence analysis

    E-Print Network [OSTI]

    Gao, Jianbo

    Recurrence time statistics: Versatile tools for genomic DNA sequence analysis Yinhe Cao1, Wen, and the genomes of many other organisms waiting to be sequenced, it has become increasingly important to develop from DNA sequences. One of the more important structures in a DNA se- quence is repeat-related. Often

  13. Correct and incorrect nucleotide incorporation pathways in DNA polymerase b

    E-Print Network [OSTI]

    Schlick, Tamar

    Correct and incorrect nucleotide incorporation pathways in DNA polymerase b Ravi Radhakrishnan a nucleotide incorporations in the DNA by using a novel protocol involving energy minimizations, dynamics simu- sive transient intermediates, for nucleotide incorporation at the template/primer DNA junction. A large

  14. Structure of the SSB?DNA polymerase III interface and its role in DNA replication

    SciTech Connect (OSTI)

    Marceau, Aimee H.; Bahng, Soon; Massoni, Shawn C.; George, Nicholas P.; Sandler, Steven J.; Marians, Kenneth J.; Keck, James L. (MSKCC); (UMASS, Amherst); (UW-MED)

    2012-05-22T23:59:59.000Z

    Interactions between single-stranded DNA-binding proteins (SSBs) and the DNA replication machinery are found in all organisms, but the roles of these contacts remain poorly defined. In Escherichia coli, SSB's association with the {chi} subunit of the DNA polymerase III holoenzyme has been proposed to confer stability to the replisome and to aid delivery of primers to the lagging-strand DNA polymerase. Here, the SSB-binding site on {chi} is identified crystallographically and biochemical and cellular studies delineate the consequences of destabilizing the {chi}/SSB interface. An essential role for the {chi}/SSB interaction in lagging-strand primer utilization is not supported. However, sequence changes in {chi} that block complex formation with SSB lead to salt-dependent uncoupling of leading- and lagging-strand DNA synthesis and to a surprising obstruction of the leading-strand DNA polymerase in vitro, pointing to roles for the {chi}/SSB complex in replisome establishment and maintenance. Destabilization of the {chi}/SSB complex in vivo produces cells with temperature-dependent cell cycle defects that appear to arise from replisome instability.

  15. Can we model DNA at the mesoscale ? Comment on: Fluctuations in the DNA double helix: A critical review

    E-Print Network [OSTI]

    Peyrard, Michel

    2015-01-01T23:59:59.000Z

    Comment on "Fluctuations in the DNA double helix: A critical review" by Frank-Kamenetskii and Prakash

  16. DNA digestion protocol & hints Overview: Although it is pretty standard to digest DNA with restriction enzymes, here

    E-Print Network [OSTI]

    Doering, Tamara

    Liu 4/2004 DNA digestion protocol & hints Overview: Although it is pretty standard to digest DNA in molecular biology (3.1.1-3.1.2) Materials: DNA sample in water or TE buffer 10x digestion buffer.1 to 4 g 10x Digestion buffer 2 l 5 l Enzyme ? ? Water Rest of volume Rest of volume 2. Add the enzyme

  17. Preparation of DNA-containing extract for PCR amplification

    DOE Patents [OSTI]

    Dunbar, John M.; Kuske, Cheryl R.

    2006-07-11T23:59:59.000Z

    Environmental samples typically include impurities that interfere with PCR amplification and DNA quantitation. Samples of soil, river water, and aerosol were taken from the environment and added to an aqueous buffer (with or without detergent). Cells from the sample are lysed, releasing their DNA into the buffer. After removing insoluble cell components, the remaining soluble DNA-containing extract is treated with N-phenacylthiazolium bromide, which causes rapid precipitation of impurities. Centrifugation provides a supernatant that can be used or diluted for PCR amplification of DNA, or further purified. The method may provide a DNA-containing extract sufficiently pure for PCR amplification within 510 minutes.

  18. Stepped electrophoresis for movement and concentration of DNA

    DOE Patents [OSTI]

    Miles, Robin R.; Wang, Amy Wei-Yun; Mariella, Jr., Raymond P.

    2005-03-15T23:59:59.000Z

    A fluidic channel patterned with a series of thin-film electrodes makes it possible to move and concentrate DNA in a fluid passing through the fluidic channel. The DNA has an inherent negative charge and by applying a voltage between adjacent electrodes the DNA is caused to move. By using a series of electrodes, when one electrode voltage or charge is made negative with respect to adjacent electrodes, the DNA is repelled away from this electrode and attached to a positive charged electrode of the series. By sequentially making the next electrode of the series negative, the DNA can be moved to and concentrated over the remaining positive electrodes.

  19. T4 DNA condensation in water-alcohol media

    E-Print Network [OSTI]

    M. O. Gallyamov; O. A. Pyshkina; V. G. Sergeyev; I. V. Yaminsky

    2011-07-21T23:59:59.000Z

    The process of compaction of high molecular weight DNA T4 is investigated directly in a AFM liquid cell. The AFM-images of globules formed by DNA molecules in the result of compaction in water-alcohol environments at high izopropanol concentration (80%) are received; it is found that at intermediate concentration of izopropanol (40-50%) the DNA molecules form partially compacted formations in which the separate coils of macromolecules twist in toroidal structures. It is shown using the technique of deconvolution of the AFM-images that the globule include only one closely packed DNA molecule. The model of DNA packing is proposed on the basis of AFM experiment.

  20. DNA-nuclear matrix interactions and ionizing radiation sensitivity

    SciTech Connect (OSTI)

    Schwartz, J.L. (Argonne National Lab., IL (United States) Chicago Univ., IL (United States). Dept. of Radiation and Cellular Oncology); Vaughan, A.T.M. (Loyola Univ., Hines, IL (United States). Dept. of Radiotherapy)

    1993-01-01T23:59:59.000Z

    The association between inherent ionizing radiation sensitivity and DNA supercoil unwinding in mammalian cells suggests that the DNA-nuclear matrix attachment region (MAR) plays an important role in radiation response. In radioresistant cells, the MAR structure may exist in a more stable, open configuration, limiting DNA unwinding following strand break induction and maintaining DNA ends in close proximity for more rapid and accurate rejoining. In addition, the open configuration at these matrix attachment sites may serve to facilitate rapid DNA processing of breaks by providing (1) sites for repair proteins to collect and (2) energy to drive enzymatic reactions.

  1. DNA-nuclear matrix interactions and ionizing radiation sensitivity

    SciTech Connect (OSTI)

    Schwartz, J.L. [Argonne National Lab., IL (United States)]|[Chicago Univ., IL (United States). Dept. of Radiation and Cellular Oncology; Vaughan, A.T.M. [Loyola Univ., Hines, IL (United States). Dept. of Radiotherapy

    1993-03-01T23:59:59.000Z

    The association between inherent ionizing radiation sensitivity and DNA supercoil unwinding in mammalian cells suggests that the DNA-nuclear matrix attachment region (MAR) plays an important role in radiation response. In radioresistant cells, the MAR structure may exist in a more stable, open configuration, limiting DNA unwinding following strand break induction and maintaining DNA ends in close proximity for more rapid and accurate rejoining. In addition, the open configuration at these matrix attachment sites may serve to facilitate rapid DNA processing of breaks by providing (1) sites for repair proteins to collect and (2) energy to drive enzymatic reactions.

  2. Ion Runaway in Lightning Discharges

    E-Print Network [OSTI]

    Landreman, Matt

    Runaway ions can be produced in plasmas with large electric fields, where the accelerating electric force is augmented by the low mean ionic charge due to the imbalance between the number of electrons and ions. Here we ...

  3. DNA end resection by Dna2Sgs1RPA and its stimulation by Top3Rmi1 and Mre11Rad50Xrs2

    E-Print Network [OSTI]

    Kowalczykowski, Stephen C.

    LETTERS DNA end resection by Dna2Sgs1RPA and its stimulation by Top3Rmi1 and Mre11Rad50Xrs2. Campbell3 & Stephen C. Kowalczykowski1,2 The repair of DNA double-strand breaks (DSBs) by homologous to generate a 39-single- stranded DNA (ssDNA) overhang, which becomes a substrate forthe

  4. Long DNA molecule as a pseudoscalar liquid crystal

    E-Print Network [OSTI]

    K. G. Petrosyan; Chin-Kun Hu

    2009-05-13T23:59:59.000Z

    We show that a long DNA molecule can form a novel condensed phase of matter, the pseudoscalar liquid crystal, that consists of aperiodically ordered DNA fragments in right-handed B and left-handed Z forms. We discuss the possibility of transformation of B-DNA into Z-DNA and vice versa via first-order phase transitions as well as transformations from the phase with zero total chirality into pure B- or Z-DNA samples through second-order phase transitions. The presented minimalistic phenomenological model describes the pseudoscalar liquid crystal phase of DNA and the phase transition phenomena. We point out to a possibility that a pseudoscalar liquid nano-crystal can be assembled via DNA-programming.

  5. Distinct kinetics of human DNA ligases I, IIIalpha, IIIbeta, and IV reveal direct DNA sensing ability and differential physiological functions in DNA repair

    SciTech Connect (OSTI)

    Chen, Xi; Ballin, Jeff D.; Della-Maria, Julie; Tsai, Miaw-Sheue; White, Elizabeth J.; Tomkinson, Alan E.; Wilson, Gerald M.

    2009-05-11T23:59:59.000Z

    The three human LIG genes encode polypeptides that catalyze phosphodiester bond formation during DNA replication, recombination and repair. While numerous studies have identified protein partners of the human DNA ligases (hLigs), there has been little characterization of the catalytic properties of these enzymes. In this study, we developed and optimized a fluorescence-based DNA ligation assay to characterize the activities of purified hLigs. Although hLigI joins DNA nicks, it has no detectable activity on linear duplex DNA substrates with short, cohesive single-strand ends. By contrast, hLigIII{beta} and the hLigIII{alpha}/XRCC1 and hLigIV/XRCC4 complexes are active on both nicked and linear duplex DNA substrates. Surprisingly, hLigIV/XRCC4, which is a key component of the major non-homologous end joining (NHEJ) pathway, is significantly less active than hLigIII on a linear duplex DNA substrate. Notably, hLigIV/XRCC4 molecules only catalyze a single ligation event in the absence or presence of ATP. The failure to catalyze subsequent ligation events reflects a defect in the enzyme-adenylation step of the next ligation reaction and suggests that, unless there is an in vivo mechanism to reactivate DNA ligase IV/XRCC4 following phosphodiester bond formation, the cellular NHEJ capacity will be determined by the number of adenylated DNA ligaseIV/XRCC4 molecules.

  6. Hole interactions with molecular vibrations on DNA

    E-Print Network [OSTI]

    A. Omerzu; M. Licer; T. Mertelj; V. V. Kabanov; D. Mihailovic

    2004-05-13T23:59:59.000Z

    We report on a study of the interactions between holes and molecular vibrations on dry DNA using photoinduced infrared absorption spectroscopy. Laser photoexcited (PE) holes are found to have a room-temperature lifetime in excess of 1 ms, clearly indicating the presence of localization. However, from a quantitative model analysis of the frequency shifts of vibrational modes caused by the PE holes, we find the holevibrational coupling constant to be relatively small, 0.2. This interaction leads to a change in the conformational energy of 0.015 eV, which is too small to cause selftrapping at room temperature. We conclude that, at least in the dry (A) form, DNA is best understood in terms of a double chain of coupled quantum dots arising from the pseudo-random chain sequence of base pairs, in which Anderson localization prevents the formation of a metallic state.

  7. Conditions for positioning of nucleosomes on DNA

    E-Print Network [OSTI]

    Sheinman, Michael

    2015-01-01T23:59:59.000Z

    Positioning of nucleosomes along eukaryotic genomes plays an important role in their organization and regulation. There are many different factors affecting the location of nucleosomes. Some can be viewed as preferential binding of a single nucleosome to different locations along the DNA and some as interactions between neighboring nucleosomes. In this study we analyzed how well nucleosomes are positioned along the DNA as a function of strength of the preferential binding, correlation length of the binding energy landscape, interactions between neighboring nucleosomes and others relevant system properties. We analyze different scenarios: designed energy landscapes and generically disordered ones and derive conditions for good positioning. Using analytic and numerical approaches we find that, even if the binding preferences are very weak, synergistic interplay between the interactions and the binding preferences is essential for a good positioning of nucleosomes, especially on correlated energy landscapes. Ana...

  8. Fast DNA Sequencing via Transverse Electronic Transport

    E-Print Network [OSTI]

    Johan Lagerqvist; Michael Zwolak; Massimiliano Di Ventra

    2006-03-01T23:59:59.000Z

    A rapid and low-cost method to sequence DNA would usher in a revolution in medicine. We propose and theoretically show the feasibility of a protocol for sequencing based on the distributions of transverse electrical currents of single-stranded DNA while it translocates through a nanopore. Our estimates, based on the statistics of these distributions, reveal that sequencing of an entire human genome could be done with very high accuracy in a matter of hours without parallelization, e.g., orders of magnitude faster than present techniques. The practical implementation of our approach would represent a substantial advancement in our ability to study, predict and cure diseases from the perspective of the genetic makeup of each individual.

  9. Bis[(1S)-1 4-azanediyl-1-(9-deazaadenin-9-yl)-1 4-dideoxy-5-methylsulfanyl-D-ribitol] tetrakis(hydrochloride) monohydrate: structure DFT energy and ligand docking results of a potent methylthioadenosine phosphorylase inhibitor found in different

    SciTech Connect (OSTI)

    G Gainsford; G Evans; K Johnston; M Seth

    2011-12-31T23:59:59.000Z

    The title compound, abbreviated as 5'ThiomethylImmA, is a potent inhibitor of methylthioadenosine phosphorylase [Singh et al. (2004). Biochemistry, 43, 9-18]. The synchrotron study reported here shows that the hydrochloride salt crystallizes with two independent, nearly superimposable, dications as a monohydrate with formula 2C{sub 12}H{sub 19}N{sub 5}O{sub 2}S{sup 2+}{center_dot}4Cl{sup -}{center_dot}H{sub 2}O. Hydrogen bonding utilizing the H atoms of the dication is found to favor certain molecular conformations in the salt, which are significantly different from those found as bound in the enzyme. Ligand docking studies starting from either of these dications or related neutral structures successfully place the conformationally revised structures in the enzyme active site but only under particular hydrogen-bonding and molecular flexibility criteria. Density functional theory calculations verify the energy similarity of the indendent cations and confirm the significant energy cost of the required conformation change to the enzyme bound form. The results suggest the using crystallographically determined free ligand coordinates as starting parameters for modelling may have serious limitations.

  10. Determining orientation and direction of DNA sequences

    DOE Patents [OSTI]

    Goodwin, Edwin H. (Los Alamos, NM); Meyne, Julianne (Los Alamos, NM)

    2000-01-01T23:59:59.000Z

    Determining orientation and direction of DNA sequences. A method by which fluorescence in situ hybridization can be made strand specific is described. Cell cultures are grown in a medium containing a halogenated nucleotide. The analog is partially incorporated in one DNA strand of each chromatid. This substitution takes place in opposite strands of the two sister chromatids. After staining with the fluorescent DNA-binding dye Hoechst 33258, cells are exposed to long-wavelength ultraviolet light which results in numerous strand nicks. These nicks enable the substituted strand to be denatured and solubilized by heat, treatment with high or low pH aqueous solutions, or by immersing the strands in 2.times.SSC (0.3M NaCl+0.03M sodium citrate), to name three procedures. It is unnecessary to enzymatically digest the strands using Exo III or another exonuclease in order to excise and solubilize nucleotides starting at the sites of the nicks. The denaturing/solubilizing process removes most of the substituted strand while leaving the prereplication strand largely intact. Hybridization of a single-stranded probe of a tandem repeat arranged in a head-to-tail orientation will result in hybridization only to the chromatid with the complementary strand present.

  11. Phylogenetic Analysis of Shewanella Strains by DNA Relatedness Derived from Whole Genome Microarray DNA-DNA Hybridization and Comparison with Other Methods

    SciTech Connect (OSTI)

    Wu, Liyou; Yi, T. Y.; Van Nostrand, Joy; Zhou, Jizhong

    2010-05-17T23:59:59.000Z

    Phylogenetic analyses were done for the Shewanella strains isolated from Baltic Sea (38 strains), US DOE Hanford Uranium bioremediation site [Hanford Reach of the Columbia River (HRCR), 11 strains], Pacific Ocean and Hawaiian sediments (8 strains), and strains from other resources (16 strains) with three out group strains, Rhodopseudomonas palustris, Clostridium cellulolyticum, and Thermoanaerobacter ethanolicus X514, using DNA relatedness derived from WCGA-based DNA-DNA hybridizations, sequence similarities of 16S rRNA gene and gyrB gene, and sequence similarities of 6 loci of Shewanella genome selected from a shared gene list of the Shewanella strains with whole genome sequenced based on the average nucleotide identity of them (ANI). The phylogenetic trees based on 16S rRNA and gyrB gene sequences, and DNA relatedness derived from WCGA hybridizations of the tested Shewanella strains share exactly the same sub-clusters with very few exceptions, in which the strains were basically grouped by species. However, the phylogenetic analysis based on DNA relatedness derived from WCGA hybridizations dramatically increased the differentiation resolution at species and strains level within Shewanella genus. When the tree based on DNA relatedness derived from WCGA hybridizations was compared to the tree based on the combined sequences of the selected functional genes (6 loci), we found that the resolutions of both methods are similar, but the clustering of the tree based on DNA relatedness derived from WMGA hybridizations was clearer. These results indicate that WCGA-based DNA-DNA hybridization is an idea alternative of conventional DNA-DNA hybridization methods and it is superior to the phylogenetics methods based on sequence similarities of single genes. Detailed analysis is being performed for the re-classification of the strains examined.

  12. Probing Minor Groove Hydrogen Bonding Interactions between RB69 DNA Polymerase and DNA

    SciTech Connect (OSTI)

    Xia, Shuangluo; Christian, Thomas D.; Wang, Jimin; Konigsberg, William H. (Yale)

    2012-09-17T23:59:59.000Z

    Minor groove hydrogen bonding (HB) interactions between DNA polymerases (pols) and N3 of purines or O2 of pyrimidines have been proposed to be essential for DNA synthesis from results obtained using various nucleoside analogues lacking the N3 or O2 contacts that interfered with primer extension. Because there has been no direct structural evidence to support this proposal, we decided to evaluate the contribution of minor groove HB interactions with family B pols. We have used RB69 DNA pol and 3-deaza-2'-deoxyadenosine (3DA), an analogue of 2-deoxyadenosine, which has the same HB pattern opposite T but with N3 replaced with a carbon atom. We then determined pre-steady-state kinetic parameters for the insertion of dAMP opposite dT using primer/templates (P/T)-containing 3DA. We also determined three structures of ternary complexes with 3DA at various positions in the duplex DNA substrate. We found that the incorporation efficiency of dAMP opposite dT decreased 10{sup 2}-10{sup 3}-fold even when only one minor groove HB interaction was missing. Our structures show that the HB pattern and base pair geometry of 3DA/dT is exactly the same as those of dA/dT, which makes 3DA an optimal analogue for probing minor groove HB interactions between a DNA polymerase and a nucleobase. In addition, our structures provide a rationale for the observed 10{sup 2}-10{sup 3}-fold decrease in the rate of nucleotide incorporation. The minor groove HB interactions between position n-2 of the primer strand and RB69pol fix the rotomer conformations of the K706 and D621 side chains, as well as the position of metal ion A and its coordinating ligands, so that they are in the optinal orientation for DNA synthesis.

  13. Capstan friction model for DNA ejection from bacteriophages

    E-Print Network [OSTI]

    Sandip Ghosal

    2013-01-09T23:59:59.000Z

    Bacteriophages infect cells by attaching to the outer membrane and injecting their DNA into the cell.The phage DNA is then transcribed by the cell's transcription machinery.A number of physical mechanisms by which DNA can be translocated from the phage capsid into the cell have been identified. A fast ejection driven by the elastic and electrostatic potential energy of the compacted DNA within the viral capsid appears to be used by most phages, at least to initiate infection.In recent in vitro experiments, the speed of DNA translocation from a lambda phage capsid has been measured as a function of ejected length over the entire duration of the event.Here a mechanical model is proposed that is able to explain the observed dependence of exit velocity on ejected length, and that is also consistent with the accepted picture of the geometric arrangement of DNA within the viral capsid.

  14. How effective is graphene nanopore geometry on DNA sequencing?

    E-Print Network [OSTI]

    Satarifard, Vahid; Ejtehadi, Mohammad Reza

    2015-01-01T23:59:59.000Z

    In this paper we investigate the effects of graphene nanopore geometry on homopolymer ssDNA pulling process through nanopore using steered molecular dynamic (SMD) simulations. Different graphene nanopores are examined including axially symmetric and asymmetric monolayer graphene nanopores as well as five layer graphene polyhedral crystals (GPC). The pulling force profile, moving fashion of ssDNA, work done in irreversible DNA pulling and orientations of DNA bases near the nanopore are assessed. Simulation results demonstrate the strong effect of the pore shape as well as geometrical symmetry on free energy barrier, orientations and dynamic of DNA translocation through graphene nanopore. Our study proposes that the symmetric circular geometry of monolayer graphene nanopore with high pulling velocity can be used for DNA sequencing.

  15. Self-assembly of DNA-functionalized Colloids

    E-Print Network [OSTI]

    Panagiotis E. Theodorakis; Nikolaos G. Fytas; Gerhard Kahl; Christoph Dellago

    2015-03-18T23:59:59.000Z

    Colloidal particles grafted with single-stranded DNA (ssDNA) chains can self-assemble into a number of different crystalline structures, where hybridization of the ssDNA chains creates links between colloids stabilizing their structure. Depending on the geometry and the size of the particles, the grafting density of the ssDNA chains, and the length and choice of DNA sequences, a number of different crystalline structures can be fabricated. However, understanding how these factors contribute synergistically to the self-assembly process of DNA-functionalized nano- or micro-sized particles remains an intensive field of research. Moreover, the fabrication of long-range structures due to kinetic bottlenecks in the self-assembly are additional challenges. Here, we discuss the most recent advances from theory and experiment with particular focus put on recent simulation studies.

  16. Isolation of Discrete Nanoparticle-DNA Conjugates for Plasmonic Applications

    SciTech Connect (OSTI)

    Alivisatos, Paul; Claridge, Shelley A.; Liang, Huiyang W.; Basu, Sourav Roger; Frechet, Jean M.J.; Alivisatos, A. Paul

    2008-04-11T23:59:59.000Z

    Discrete DNA-gold nanoparticle conjugates with DNA lengths as short as 15 bases for both 5 nm and 20 nm gold particles have been purified by anion-exchange HPLC. Conjugates comprising short DNA (<40 bases) and large gold particles (>_ 20 nm) are difficult to purify by other means, and are potential substrates for plasmon coupling experiments. Conjugate purity is demonstrated by hybridizing complementary conjugates to form discrete structures, which are visualized by TEM.

  17. Transposon-containing DNA cloning vector and uses thereof

    DOE Patents [OSTI]

    Berg, Claire M. (W. Willington, CT); Berg, Douglas E. (St. Louis, MO); Wang, Gan (Storrs, CT)

    1997-01-01T23:59:59.000Z

    The present invention discloses a rapid method of restriction mapping, sequencing or localizing genetic features in a segment of deoxyribonucleic acid (DNA) that is up to 42 kb in size. The method in part comprises cloning of the DNA segment in a specialized cloning vector and then isolating nested deletions in either direction in vivo by intramolecular transposition into the cloned DNA. A plasmid has been prepared and disclosed.

  18. DNA Methylation as a Biomarker for Preeclampsia

    SciTech Connect (OSTI)

    Anderson, Cindy M.; Ralph, Jody L.; Wright, Michelle L.; Linggi, Bryan E.; Ohm, Joyce E.

    2014-10-01T23:59:59.000Z

    Background: Preeclampsia contributes significantly to pregnancy-associated morbidity and mortality as well as future risk of cardiovascular disease in mother and offspring, and preeclampsia in offspring. The lack of reliable methods for early detection limits the opportunities for prevention, diagnosis, and timely treatment. Purpose: The purpose of this study was to explore distinct DNA methylation patterns associated with preeclampsia in both maternal cells and fetal-derived tissue that represent potential biomarkers to predict future preeclampsia and inheritance in children. Method: A convenience sample of nulliparous women (N = 55) in the first trimester of pregnancy was recruited for this prospective study. Genome-wide DNA methylation was quantified in first-trimester maternal peripheral white blood cells and placental chorionic tissue from normotensive women and those with preeclampsia (n = 6/group). Results: Late-onset preeclampsia developed in 12.7% of women. Significant differences in DNA methylation were identified in 207 individual linked cytosine and guanine (CpG) sites in maternal white blood cells collected in the first trimester (132 sites with gain and 75 sites with loss of methylation), which were common to approximately 75% of the differentially methylated CpG sites identified in chorionic tissue of fetal origin. Conclusion: This study is the first to identify maternal epigenetic targets and common targets in fetal-derived tissue that represent putative biomarkers for early detection and heritable risk of preeclampsia. Findings may pave the way for diagnosis of preeclampsia prior to its clinical presentation and acute damaging effects, and the potential for prevention of the detrimental long-term sequelae.

  19. Ergonomics in DNA Sequencing: Standing Down to Ergonomics

    E-Print Network [OSTI]

    Naca, Christine

    2009-01-01T23:59:59.000Z

    Ergonomics in DNA SequencingStanding Down to Ergonomics Presented at the COEH 2009We Do: Introduction How We Work: Ergonomics Challenges in

  20. Fleet DNA Project - Data Dictionary for Public Download Files

    SciTech Connect (OSTI)

    Duran, A.; Burton, E.; Kelly, K.; Walkowicz, K.

    2014-09-01T23:59:59.000Z

    Reference document for the Fleet DNA results data shared on the NREL public website. The document includes variable definitions and descriptions to assist users in understanding data.

  1. Dynamics of extracellular DNA in the marine environment

    SciTech Connect (OSTI)

    Paul, J.H.; Jeffrey, W.H.; DeFlaun, M.F.

    1987-01-01T23:59:59.000Z

    The production and turnover of dissolved DNA in subtropical estuarine and oligotrophic oceanic environments were investigated. Actively growing heterotrophic bacterioplankton (i.e., those capable of (/sup 3/H)thymidine incorporation) were found to produce dissolved DNA, presumably through the processes of death and lysis, grazing by bacteriovores, and excretion. Production of dissolved DNA as determined by (/sup 3/H)thymidine incorporation was less than or equal to4% of the ambient dissolved DNA concentration per day. In turnover studies, the addition of (/sup 3/H)DNA (Escherichia coli chromosomal) to seawater resulted in rapid hydrolysis and uptake of radioactivity by microbial populations. DNA was hydrolyzed by both cell-associated and extracellular nucleases, in both estuarine and offshore environments. Kinetic analysis performed for a eutrophic estuary indicated a turnover time for dissolved DNA as short as 6.5 h. Microautoradiographic studies of bacterial populations in Tampa Bay indicated that filamentous and attached bacteria took up most of the radioactivity from (/sup 3/H)DNA. Dissolved DNA is therefore a dynamic component of the dissolved organic matter in the marine environment, and bacterioplankton play a key role in the cycling of this material.

  2. Nanoscale topographical replication of graphene architecture by artificial DNA nanostructures

    SciTech Connect (OSTI)

    Moon, Y.; Seo, S.; Park, J.; Park, T.; Ahn, J. R., E-mail: jrahn@skku.edu [Department of Physics, Sungkyunkwan University, Suwon 440-746 (Korea, Republic of); Shin, J.; Dugasani, S. R. [Sungkyunkwan Advanced Institute of Nanotechnology (SAINT), Sungkyunkwan University, Suwon 440-746 (Korea, Republic of); Woo, S. H. [College of Pharmacy, Chungnam National University, Daejeon 305-764 (Korea, Republic of); Park, S. H., E-mail: sunghapark@skku.edu [Department of Physics, Sungkyunkwan University, Suwon 440-746 (Korea, Republic of); Sungkyunkwan Advanced Institute of Nanotechnology (SAINT), Sungkyunkwan University, Suwon 440-746 (Korea, Republic of)

    2014-06-09T23:59:59.000Z

    Despite many studies on how geometry can be used to control the electronic properties of graphene, certain limitations to fabrication of designed graphene nanostructures exist. Here, we demonstrate controlled topographical replication of graphene by artificial deoxyribonucleic acid (DNA) nanostructures. Owing to the high degree of geometrical freedom of DNA nanostructures, we controlled the nanoscale topography of graphene. The topography of graphene replicated from DNA nanostructures showed enhanced thermal stability and revealed an interesting negative temperature coefficient of sheet resistivity when underlying DNA nanostructures were denatured at high temperatures.

  3. assemble linear dna: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    compute? In his early studies of reversible computation, Bennett imagined an enzymatic Turing Machine which modified a hetero-polymer (such as DNA) to perform computation with...

  4. assembly regulate dna: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    compute? In his early studies of reversible computation, Bennett imagined an enzymatic Turing Machine which modified a hetero-polymer (such as DNA) to perform computation with...

  5. automated dna extraction: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and Crop Cleaning PCR Products. . Labeling Cleaned... 5 56 57 . . . 57 CHAPTER IV AUTOMATION POTENTIAL . . . . . . . 59 Introduction . . Collecting Field Data . DNA Extraction....

  6. automated dna mutation: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and Crop Cleaning PCR Products. . Labeling Cleaned... 5 56 57 . . . 57 CHAPTER IV AUTOMATION POTENTIAL . . . . . . . 59 Introduction . . Collecting Field Data . DNA Extraction....

  7. Genetic Transformation and Mutagenesis Via Single-Stranded DNA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Genetic Transformation and Mutagenesis Via Single-Stranded DNA in the Unicellular, Diazotrophic Cyanobacteria of the Genus Genetic Transformation and Mutagenesis Via...

  8. Phase diagram of mechanically stretched DNA: The salt effect

    E-Print Network [OSTI]

    Amar Singh; Navin Singh

    2014-09-05T23:59:59.000Z

    The cations, in form of salt, present in the solution containing DNA play a crucial role in the opening of two strands of DNA. We use a simple non linear model and investigate the role of these cations on the mechanical unzipping of DNA. The Hamiltonian is modified to incoporate the solvent effect and the cations present in the solution. We calculate the melting temperature as well as the critical force that is required to unzip the DNA molecule as a function of salt concentration of the solution. The phase diagrams are found to be in close agreement with the experimental phase diagrams.

  9. ancient dna analysis: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    model of short circular DNA. We obtain analytic expressions for configurations, elastic energy, twist and linking number of our solutions. We find the onset of the plectonemic...

  10. Gel Electrophoresis of Gold-DNA Nano-Conjugates

    E-Print Network [OSTI]

    Pellegrino, T.; Sperling, R.A.; Alivisatos, A.P.; Parak, W.J.

    2008-01-01T23:59:59.000Z

    R. A. Sperling 1,# , A. P. Alivisatos 2 , W. J. Parak 1,2,*materials {Mirkin, 1996 #3440; Alivisatos, 1996 #3342}. DNA-

  11. ancient human dna: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    STUDY OF DNA DOUBLE-STRAND BREAKS IN BYSTANDER PRIMARY HUMAN FIBROBLASTS L. B. Smilenov-or-nothing manner(7) . Bystander cells exhibit a variety of characteristics of...

  12. ancient dna studies: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    STUDY OF DNA DOUBLE-STRAND BREAKS IN BYSTANDER PRIMARY HUMAN FIBROBLASTS L. B. Smilenov-or-nothing manner(7) . Bystander cells exhibit a variety of characteristics of...

  13. Helical Disruptions in Small Loops of DNA

    E-Print Network [OSTI]

    Zoli, Marco

    2015-01-01T23:59:59.000Z

    The thermodynamical stability of DNA minicircles is investigated by means of path integral techniques. Hydrogen bonds between base pairs on complementary strands can be broken by thermal fluctuations and temporary fluctuational openings along the double helix are essential to biological functions such as transcription and replication of the genetic information. Helix unwinding and bubble formation patterns are computed in circular sequences with variable radius in order to analyze the interplay between molecule size and appearance of helical disruptions. The latter are found in minicircles with $100$ base pairs and appear as a strategy to soften the stress due to the bending and torsion of the helix.

  14. DNA analysis conference in Santa Fe

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625govInstrumentstdmadapInactiveVisitingContract Management Fermi SitePART I SECTION ADMSE ElectronDNA analysis

  15. A DNA tweezer-actuated enzyme nanoreactor

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742EnergyOnItemResearch >InternshipDepartment ofAugustDecember8threbuildA Comprehensive LookA DNA

  16. Intriguing DNA Editor Has a Structural Trigger

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOEThe Bonneville PowerCherries 82981-1cnHigh SchoolIn OtherEnergy International Fuel7RadiativeIntriguing DNA

  17. Intriguing DNA Editor Has a Structural Trigger

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOEThe Bonneville PowerCherries 82981-1cnHigh SchoolIn OtherEnergy InternationalIntriguing DNA Editor Has a

  18. Modeling DNA Interactions in Nucleosome Particles (A Computational Study of DNA Interactions in Nucleosome Particles)

    E-Print Network [OSTI]

    Kansas, University of

    Technology Center, University of Kansas, Lawrence, KS 66045. 2006 Annual Kansas City Area Life Sciences characterize global motions of the histone-DNA complex with respect to energy fluctuations in an assessment of energy differences resulting from the nucleotide substitutions will be presented in the context

  19. Regulation of DNA Repair Fidelity by Molecular Checkpoints: "Gates" in DNA Polymerase 's Substrate Selection

    E-Print Network [OSTI]

    Schlick, Tamar

    Schlick*, Department of Chemistry and Courant Institute of Mathematical Sciences, New York UniVersity, New York, New York 10012, and Laboratory of Structural Biology, National Institute of EnVironmental Health enzymology data. Focusing on DNA polymerase (pol ), we present an emerging view of the geometric, energetic

  20. DNA Identification of Mountain Lions Involved in Livestock Predation and Public Safety Incidents and Investigations

    E-Print Network [OSTI]

    Ernest, Holly

    1 DNA Identification of Mountain Lions Involved in Livestock Predation and Public Safety Incidents concolor, bobcat, forensic, genetics, DNA techniques, noninvasive sampling, fecal DNA, prey swab DNA ABSTRACT Using three case studies, we demonstrated the utility of techniques to analyze DNA from trace

  1. Functional Characterization of Kansl2 and Its Role in Mitochondrial DNA Repair and Maintenance

    E-Print Network [OSTI]

    Liau, Wei-Siang

    2012-01-01T23:59:59.000Z

    understanding of the maintenance and repair of the mtDNA,A is necessary for mtDNA maintenance and embryogenesis in1997). Mitochondrial DNA maintenance in vertebrates. Annu

  2. Development of new tools for the production of plasmid DNA biopharmaceuticals

    E-Print Network [OSTI]

    Bower, Diana M. (Diana Morgan)

    2012-01-01T23:59:59.000Z

    DNA vaccines and gene therapies that use plasmid DNA (pDNA) as a vector have gained attention in recent years for their good safety profile, ease of manufacturing, and potential to treat a host of diseases. With this ...

  3. A DNA Damage-Induced, SOS-Independent Checkpoint Regulates Cell Division in Caulobacter crescentus

    E-Print Network [OSTI]

    Modell, Joshua W.

    Cells must coordinate DNA replication with cell division, especially during episodes of DNA damage. The paradigm for cell division control following DNA damage in bacteria involves the SOS response where cleavage of the ...

  4. Morphological taxonomy, DNA barcoding, and species diversity in southern Rocky Mountain headwater streams

    E-Print Network [OSTI]

    Zamudio, Kelly R.

    Morphological taxonomy, DNA barcoding, and species diversity in southern Rocky Mountain headwater and Conditions #12;MOLECULAR APPROACHES IN FRESHWATER ECOLOGY Morphological taxonomy, DNA barcoding, and species: diversity, elevation, DNA barcoding, taxonomy, aquatic insect, EPT, southern Rocky Mountain Elevation

  5. Superconducting Nanowires Fabricated Using DNA and Nanotubes as Molecular Templates

    E-Print Network [OSTI]

    Goldbart, Paul M.

    functions of DNA have been demonstrated in recent experiments in which a linear arrangement of nanoparticles acid molecules or suspended single-wall carbon nanotubes as templates for making superconducting Introduction Deoxyribonucleic acid (DNA) contains genetic instructions used in the develop- ment

  6. Alternative Methods for Human Identification: Mitochondrial DNA Base Composition Profiling

    E-Print Network [OSTI]

    Perkins, Richard A.

    of nucleotides Base composition of a DNA molecule can be inferred An empirical formula of numbers of A, G, C) Fully automated Plate stacker holds up to 15 PCR plates Desalting by magnetic bead cleanup Cleanup are dissociated on injection DNA molecular masses are measured Forward and reverse strands measured separately

  7. Conserved and Unconventional Responses to DNA Damage in Tetrahymena

    E-Print Network [OSTI]

    Sandoval Oporto, Pamela

    2012-07-16T23:59:59.000Z

    characterization of the response to genotoxic agents showed that Tetrahymena is able to activate a G1/S and intra-S phase DNA damage response. The results presented here suggest that a caffeine-dependent checkpoint activator protein modulates the response to DNA...

  8. Yeast Genomic Library Genomic DNA Sau3AI partial digestion

    E-Print Network [OSTI]

    Odorizzi, Greg

    Yeast Genomic Library Concept: Genomic DNA Sau3AI partial digestion Vector DNA BamHI full digestion partial Ligate and transform above products Vector Information: use centromeric plasmid to avoid of the mcs Preparing Vector: 1) digest 3-4ug of library vector with BamHI for 2-4hrs in a total volume of 20

  9. DNA barcoding and the renaissance of taxonomy Scott E. Miller*

    E-Print Network [OSTI]

    DNA barcoding and the renaissance of taxonomy Scott E. Miller* Office of the Under Secretary representing field ecology, molecular genetics, and morphological taxonomy presents data in this issue of PNAS). The work of Smith et al. (2) is a display of integrated taxonomy, demon- strating how DNA barcoding

  10. Energy and Technology Review: Unlocking the mysteries of DNA repair

    SciTech Connect (OSTI)

    Quirk, W.A.

    1993-04-01T23:59:59.000Z

    DNA, the genetic blueprint, has the remarkable property of encoding its own repair following diverse types of structural damage induced by external agents or normal metabolism. We are studying the interplay of DNA damaging agents, repair genes, and their protein products to decipher the complex biochemical pathways that mediate such repair. Our research focuses on repair processes that correct DNA damage produced by chemical mutagens and radiation, both ionizing and ultraviolet. The most important type of DNA repair in human cells is called excision repair. This multistep process removes damaged or inappropriate pieces of DNA -- often as a string of 29 nucleotides containing the damage -- and replaces them with intact ones. We have isolated, cloned, and mapped several human repair genes associated with the nucleotide excision repair pathway and involved in the repair of DNA damage after exposure to ultraviolet light or mutagens in cooked food. We have shown that a defect in one of these repair genes, ERCC2, is responsible for the repair deficiency in one of the groups of patients with the recessive genetic disorder xeroderma pigmentosum (XP group D). We are exploring ways to purify sufficient quantities (milligrams) of the protein products of these and other repair genes so that we can understand their functions. Our long-term goals are to link defective repair proteins to human DNA repair disorders that predispose to cancer, and to produce DNA-repair-deficient mice that can serve as models for the human disorders.

  11. Semiflexible Polymers: Fundamental Theory and Applications in DNA Packaging

    E-Print Network [OSTI]

    Straight, Aaron

    Semiflexible Polymers: Fundamental Theory and Applications in DNA Packaging Thesis by Andrew James of perfectly flexible and perfectly rigid polymer chains; however, many polymers, for example DNA, are somewhere in between these two limiting cases. Such polymers are termed semiflexible, and their molecular

  12. cDNA encoding a polypeptide including a hevein sequence

    DOE Patents [OSTI]

    Raikhel, N.V.; Broekaert, W.F.; Namhai Chua; Kush, A.

    1993-02-16T23:59:59.000Z

    A cDNA clone (HEV1) encoding hevein was isolated via polymerase chain reaction (PCR) using mixed oligonucleotides corresponding to two regions of hevein as primers and a Hevea brasiliensis latex cDNA library as a template. HEV1 is 1,018 nucleotides long and includes an open reading frame of 204 amino acids.

  13. Charge Transfer Across the Nanocrystalline-DNA Interface: Probing

    E-Print Network [OSTI]

    Scherer, Norbert F.

    matched DNA. The initial charge separation sequence in these hybrid bioinor- ganic nanocomposites, Illinois 60637 Received February 26, 2004; Revised Manuscript Received March 26, 2004 ABSTRACT Hybrid nanocomposites that electronically link TiO2 nanoparticles to DNA oligonucleotides were developed. The linking

  14. Conformational Manipulation of DNA in Nanochannels Using Hydrodynamics

    E-Print Network [OSTI]

    Qihao He; Hubert Ranchon; Pascal Carrivain; Yannick Viero; Joris Lacroix; Charline Blatch; Emmanuelle Daran; Jean-Marc Victor; Aurlien Bancaud

    2014-09-01T23:59:59.000Z

    The control over DNA elongation in nanofluidic devices holds great potential for large-scale genomic analysis. So far, the manipulation of DNA in nanochannels has been mostly carried out with electrophoresis and seldom with hydrodynamics, although the physics of soft matter in nanoscale flows has raised considerable interest over the past decade. In this report the migration of DNA is studied in nanochannels of lateral dimension spanning 100 to 500 nm using both actuation principles. We show that the relaxation kinetics are 3-fold slowed down and the extension increases up to 3-fold using hydrodynamics. We propose a model to account for the onset in elongation with the flow, which assumes that DNA response is determined by the shear-driven lift forces mediated by the proximity of the channels' walls. Overall, we suggest that hydrodynamic actuation allows for an improved manipulation of DNA in nanochannels.

  15. The Glutamate Switch of Bacteriophage T7 DNA Helicase ROLE IN COUPLING NUCLEOTIDE TRIPHOSPHATE (NTP) AND DNA BINDING TO NTP

    E-Print Network [OSTI]

    Richardson, Charles C.

    The Glutamate Switch of Bacteriophage T7 DNA Helicase ROLE IN COUPLING NUCLEOTIDE TRIPHOSPHATE (NTP also creates nucleotide-binding sites located at the interfaces of the sub- units. DNA binding of a bound nucleoside 5 -triphosphate. However, in the absence of a nucleotide, Glu-343 changes orientation

  16. Influence of 8-Oxoguanosine on the Fine Structure of DNA Studied...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    8-Oxoguanosine on the Fine Structure of DNA Studied with Biasing-Potential Replica Exchange Simulations. Influence of 8-Oxoguanosine on the Fine Structure of DNA Studied with...

  17. Folding of a DNA Hairpin Loop Structure in Explicit SolventUsingRepli...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of a DNA Hairpin Loop Structure in Explicit Solvent UsingReplica-Exchange Molecular Dynamics Simulations. Folding of a DNA Hairpin Loop Structure in Explicit Solvent...

  18. Sensitive method for measurement of telomeric DNA content in human tissues

    DOE Patents [OSTI]

    Bryant, Jennifer E. (Albuquerque, NM); Hutchings, Kent G. (Albuquerque, NM); Moyzis, Robert K. (Corona Del Mar, CA); Griffith, Jeffrey K. (Cedar Crest, NM)

    1999-02-16T23:59:59.000Z

    A sensitive method for measurement of telomeric DNA content in human tissue, based upon the ratio of telomeric to centromeric DNA present in the tissue.

  19. Information weights of nucleotides in DNA sequences

    E-Print Network [OSTI]

    M. R. Dudek; S. Cebrat; M. Kowalczuk; P. Mackiewicz; A. Nowicka; D. Mackiewicz; M. Dudkiewicz

    2003-01-21T23:59:59.000Z

    The coding sequence in DNA molecule is considered as a message to be transferred to receiver, the proteins, through a noisy information channel and each nucleotide is assigned a respective information weight. With the help of the nucleotide substitution matrix we estimated the lower bound of the amount of information carried out by nucleotides which is not subject of mutations. We used the calculated weights to reconstruct k-oligomers of genes from the Borrelia burgdorferi genome. We showed, that to this aim there is sufficient a simple rule, that the number of bits of the carried information cannot exceed some threshold value. The method introduced by us is general and applies to every genome.

  20. Dellaporta DNA Extraction Citation: Stephen L. Dellaporta,Jonathan Wood , James B. Hicks. A plant DNA

    E-Print Network [OSTI]

    Wurtele, Eve Syrkin

    ice) for 20 min. 7. Spin tubes at 13K rpm for 20 min in a Sorval centrifuge with the SA-600 rotor (~25 at 12K rpm for 15 min in a Sorval centrifuge with the SA-600 rotor (~20,000 x g). 11. Gently pour off. The most efficient number of samples for Dellaporta DNA Isolation is 12 each time due to centrifuge rotor