Powered by Deep Web Technologies
Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


1

Geothermal Literature Review At Lightning Dock Geothermal Area...  

Open Energy Info (EERE)

Home Exploration Activity: Geothermal Literature Review At Lightning Dock Geothermal Area (Lienau, 1990) Exploration Activity Details Location Lightning Dock Geothermal Area...

2

Geothermal Literature Review At Lightning Dock Geothermal Area...  

Open Energy Info (EERE)

Geothermal Literature Review At Lightning Dock Geothermal Area (Smith, 1978) Exploration Activity Details Location Lightning Dock Geothermal Area Exploration Technique Geothermal...

3

Thermal Gradient Holes At Lightning Dock Geothermal Area (Cunniff...  

Open Energy Info (EERE)

Thermal Gradient Holes At Lightning Dock Geothermal Area (Cunniff & Bowers, 2005) Exploration Activity Details Location Lightning Dock Geothermal Area Exploration Technique Thermal...

4

Analytical Modeling At Lightning Dock Geothermal Area (Brook...  

Open Energy Info (EERE)

Modeling At Lightning Dock Geothermal Area (Brook, Et Al., 1978) Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Analytical Modeling At Lightning...

5

Geographic Information System At Lightning Dock Geothermal Area...  

Open Energy Info (EERE)

Getman, 2014) Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Geographic Information System At Lightning Dock Geothermal Area (Getman, 2014)...

6

Geothermal Literature Review At Lightning Dock Geothermal Area...  

Open Energy Info (EERE)

Evidence for Large-Scale Laramide Tectonic Inversion and a Mid-Tertiary Caldera Ring Fracture Zone at the Lightning Dock Geothermal System, New Mexico Additional References...

7

Geothermal Literature Review At Lightning Dock Geothermal Area...  

Open Energy Info (EERE)

Rafferty, 1997) Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Geothermal Literature Review At Lightning Dock Geothermal Area (Rafferty, 1997)...

8

Lightning Dock Geothermal Area | Open Energy Information  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data CenterFranconia, Virginia: Energy Resources Jump to:46 - 429Lacey,(Monaster And Coolbaugh, 2007) JumpDesign Jump to: navigation,Lightning

9

Aeromagnetic Survey At Lightning Dock Area (Cunniff & Bowers, 2005) | Open  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Office of InspectorConcentrating SolarElectricEnergy Information Lightning Dock Area (Cunniff & Bowers, 2005)

10

Evaluation of Geothermal Potential of Lightning Dock KGRA, New Mexico |  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home5b9fcbce19 NoPublic Utilities Address:011-DNA Jump37. It isInformationexplains a4 ClimateEtrionPower Pvt LtdAOpen Energy

11

Direct-Current Resistivity Survey At Lightning Dock Area (Cunniff & Bowers,  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home5b9fcbce19 NoPublic Utilities Address:011-DNA Jump to:52c8ff988c1Dering Harbor, NewRidge,Dinwiddie(FURUMOTO, 1976) |2005) | Open

12

Geophysics, Geology and Geothermal Leasing Status of the Lightning...  

Open Energy Info (EERE)

Leasing Status of the Lightning Dock KGRA, Animas Valley, New Mexico Author C. Smith Published New Mexico Geological Society Guidebook, 1978 DOI Not Provided Check for DOI...

13

Lightning Dock Geothermal Space Heating Project: Lightning Dock KGRA, New  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home5b9fcbce19 No revision hasInformation Earth's Heat JumpInc Place:KeystoneSolar (Texas) Jump to: navigation,Beach

14

Lightning Dock Geothermal Space Heating Project: Lightning Dock...  

Open Energy Info (EERE)

geothermal greenhouse and home heating systems, which consisted of pumping geothermal water and steam through passive steam heaters, and convert the systems to one using modern...

15

Lightning Dock Geothermal Area | Open Energy Information  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data CenterFranconia, Virginia: Energy Resources Jump to:46 - 429Lacey,(Monaster And Coolbaugh, 2007) JumpDesign Jump to: navigation,

16

Lightning Dock Geothermal Facility | Open Energy Information  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home5b9fcbce19 No revision hasInformation Earth's Heat JumpInc Place:KeystoneSolar (Texas) Jump to: navigation,Beach Jump

17

Compound and Elemental Analysis At Lightning Dock Geothermal...  

Open Energy Info (EERE)

resource Notes The chemical analysis of water samples was used to try and identify a source reservoir temperature and reservoir rock type. References F. Dellechaie (1977) A...

18

Lightning Dock KGRA, New Mexico's Largest Geothermal Greenhouse...  

Open Energy Info (EERE)

Largest Geothermal Greenhouse, Largest Aquaculture Facility, and First Binary Electrical Power Plant. Geo-Heat Center Bulletin. 23:37-41. Related Geothermal Exploration Activities...

19

Geothermal Literature Review At Lightning Dock Geothermal Area...  

Open Energy Info (EERE)

the new powerplant was brought online. The study was initiated during a previous NREL student competition, and then the research was expanded and published. References S. Dahal,...

20

Direct-Current Resistivity Survey At Lightning Dock Area (Warpinski...  

Open Energy Info (EERE)

Survey Activity Date Usefulness not indicated DOE-funding Unknown Notes As a foundation for successful siting and drilling a deep test well, additional geophysical work has...

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


21

Compound and Elemental Analysis At Lightning Dock Geothermal...  

Open Energy Info (EERE)

Resource Evaluation and Definition (GRED) Program administered by DOE-AAO under Cooperative Agreement Number DE-FC07-00AL66977 Notes This project deepened a well and took...

22

Geothermal Literature Review At Lightning Dock Geothermal Area...  

Open Energy Info (EERE)

literature and how it affects access to land and mineral rights for geothermal energy production References B. C. Farhar (2002) Geothermal Access to Federal and Tribal Lands: A...

23

Geology and geothermal waters of Lightning Dock region, Animas...  

Open Energy Info (EERE)

(70 to 115.5sup 0C) seem to be structurally controlled by intersections of the ring-fracture zone of an Oligocene ash-flow tuff cauldron (Muir cauldron), a Miocene-to-Holocene...

24

Thermal Gradient Holes At Lightning Dock Geothermal Area (Cunniff...  

Open Energy Info (EERE)

R.A. Cunniff, R.L. Bowers (2003) Final Report: Enhanced Geothermal Systems Technology Phase II: Animas Valley, New Mexico Additional References Retrieved from "http:...

25

Direct-Current Resistivity Survey At Lightning Dock Area (Cunniff...  

Open Energy Info (EERE)

traverse, but was offset northward more than 500 feet to avoid the producing geothermal wells and other manmade cultural effects in the area. The locations for these two...

26

Lightning Dock II Geothermal Project | Open Energy Information  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data CenterFranconia, Virginia: Energy Resources Jump to:46 - 429Lacey,(Monaster And Coolbaugh, 2007) JumpDesign Jump to:

27

Lightning Dock KGRA, New Mexico's Largest Geothermal Greenhouse, Largest  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data CenterFranconia, Virginia: Energy Resources Jump to:46 - 429Lacey,(Monaster And Coolbaugh, 2007) JumpDesign Jump to:Aquaculture Facility,

28

Lightning Dock Geothermal HI-01 | Open Energy Information  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home5b9fcbce19 No revision hasInformation Earth's Heat JumpInc Place:KeystoneSolar (Texas) Jump to: navigation,Beach JumpHI-01

29

Lightning Dock I Geothermal Project | Open Energy Information  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home5b9fcbce19 No revision hasInformation Earth's Heat JumpInc Place:KeystoneSolar (Texas) Jump to: navigation,BeachProject Jump

30

Compound and Elemental Analysis At Lightning Dock Geothermal Area  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home5b9fcbce19 NoPublic Utilities Address: 160Benin:EnergyWisconsin: Energy,(EC-LEDS)ColumbusDHeat Ltd2002) |Al., 1996)|Al.,

31

Compound and Elemental Analysis At Lightning Dock Geothermal Area (Elston,  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home5b9fcbce19 NoPublic Utilities Address: 160Benin:EnergyWisconsin: Energy,(EC-LEDS)ColumbusDHeat Ltd2002) |Al., 1996)|Al.,Et Al.,

32

Compound and Elemental Analysis At Lightning Dock Geothermal Area (Witcher,  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home5b9fcbce19 NoPublic Utilities Address: 160Benin:EnergyWisconsin: Energy,(EC-LEDS)ColumbusDHeat Ltd2002) |Al., 1996)|Al.,Et

33

Water Sampling At Lightning Dock Geothermal Area (Swanberg, 1976) | Open  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data CenterFranconia, Virginia:FAQ < RAPID Jump to:Seadov PtyInformationSEDS dataIndiana:CoopWaspa JumpHeber Area

34

Well Deepening At Lightning Dock Geothermal Area (Witcher, 2006) | Open  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data CenterFranconia, Virginia:FAQ < RAPID Jump to:Seadov PtyInformationSEDSWawarsing,Webb County, Texas:75692¬į,WedellWelcomeEnergy

35

Ground Gravity Survey At Lightning Dock Geothermal Area (Swanberg, 1976) |  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data CenterFranconia, Virginia: Energy Resources Jump to: navigation,Ohio:Greer County is a county inAl., 1979) | Open EnergyOpen EnergyOpenOpen

36

Production Wells At Lightning Dock Geothermal Area (Cyrq Energy, 2014) |  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home5b9fcbce19 No revision hasInformation Earth'sOklahoma/GeothermalOrangePeru:JobInformationInformationOpen Energy

37

Water Sampling At Lightning Dock Geothermal Area (Witcher, 2006) | Open  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home5b9fcbce19 NoPublic Utilities Address: 160 East 300 South Place: Salt LakeWashtenaw County,Energy Information Witcher, 2006)

38

Geographic Information System At Lightning Dock Geothermal Area (Getman,  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data CenterFranconia, Virginia: Energy Resources Jump to: navigation, searchGeauga County, Ohio: EnergySector:2008) | Open Energy(Nash, Et

39

Geophysics, Geology and Geothermal Leasing Status of the Lightning Dock  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data CenterFranconia, Virginia: Energy Resources Jump to: navigation, searchGeauga County, Ohio: EnergySector:2008)theVolcanoKGRA, Animas

40

Geothermal Literature Review At Lightning Dock Geothermal Area (Callender,  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data CenterFranconia, Virginia: Energy Resources Jump to: navigation, searchGeauga County,Information(EC-LEDS)Et Al.,(Ranalli &2004)

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


41

Geothermal Literature Review At Lightning Dock Geothermal Area (Dahal, Et  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data CenterFranconia, Virginia: Energy Resources Jump to: navigation, searchGeauga County,Information(EC-LEDS)Et Al.,(Ranalli &2004)Al.,

42

Geothermal Literature Review At Lightning Dock Geothermal Area (Elston, Et  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data CenterFranconia, Virginia: Energy Resources Jump to: navigation, searchGeauga County,Information(EC-LEDS)Et Al.,(Ranalli

43

Geothermal Literature Review At Lightning Dock Geothermal Area (Farhar,  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data CenterFranconia, Virginia: Energy Resources Jump to: navigation, searchGeauga County,Information(EC-LEDS)Et Al.,(Ranalli2002) | Open

44

Geothermal Literature Review At Lightning Dock Geothermal Area (Fleischman,  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data CenterFranconia, Virginia: Energy Resources Jump to: navigation, searchGeauga County,Information(EC-LEDS)Et Al.,(Ranalli2002) |

45

Geothermal Literature Review At Lightning Dock Geothermal Area (Grant,  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data CenterFranconia, Virginia: Energy Resources Jump to: navigation, searchGeauga County,Information(EC-LEDS)Et Al.,(Ranalli2002) |1978) |

46

Geothermal Literature Review At Lightning Dock Geothermal Area (Lienau,  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data CenterFranconia, Virginia: Energy Resources Jump to: navigation, searchGeauga County,Information(EC-LEDS)Et Al.,(Ranalli2002) |1978)

47

Geothermal Literature Review At Lightning Dock Geothermal Area (Parker &  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data CenterFranconia, Virginia: Energy Resources Jump to: navigation, searchGeauga County,Information(EC-LEDS)Et Al.,(Ranalli2002)

48

Geothermal Literature Review At Lightning Dock Geothermal Area (Rafferty,  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data CenterFranconia, Virginia: Energy Resources Jump to: navigation, searchGeauga County,Information(EC-LEDS)Et Al.,(Ranalli2002)1997) |

49

Geothermal Literature Review At Lightning Dock Geothermal Area (Sammel,  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data CenterFranconia, Virginia: Energy Resources Jump to: navigation, searchGeauga County,Information(EC-LEDS)Et Al.,(Ranalli2002)1997)

50

Geothermal Literature Review At Lightning Dock Geothermal Area (Schochet,  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data CenterFranconia, Virginia: Energy Resources Jump to: navigation, searchGeauga County,Information(EC-LEDS)Et Al.,(Ranalli2002)1997)Et

51

Geothermal Literature Review At Lightning Dock Geothermal Area (Smith,  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data CenterFranconia, Virginia: Energy Resources Jump to: navigation, searchGeauga County,Information(EC-LEDS)Et

52

Geothermal Literature Review At Lightning Dock Geothermal Area (Spiegel,  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data CenterFranconia, Virginia: Energy Resources Jump to: navigation, searchGeauga County,Information(EC-LEDS)Et1957) | Open Energy

53

Geothermal Literature Review At Lightning Dock Geothermal Area (Stone, Et  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data CenterFranconia, Virginia: Energy Resources Jump to: navigation, searchGeauga County,Information(EC-LEDS)Et1957) | Open EnergyAl.,

54

Geothermal Literature Review At Lightning Dock Geothermal Area (Summers,  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data CenterFranconia, Virginia: Energy Resources Jump to: navigation, searchGeauga County,Information(EC-LEDS)Et1957) | Open EnergyAl.,1976)

55

Geothermal Literature Review At Lightning Dock Geothermal Area (Witcher,  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data CenterFranconia, Virginia: Energy Resources Jump to: navigation, searchGeauga County,Information(EC-LEDS)Et1957) | Open

56

Geothermal Literature Review At Lightning Dock Geothermal Area (Witcher,  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data CenterFranconia, Virginia: Energy Resources Jump to: navigation, searchGeauga County,Information(EC-LEDS)Et1957) | Open2008) | Open

57

Geothermal Literature Review At Lightning Dock Geothermal Area (Witcher, Et  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data CenterFranconia, Virginia: Energy Resources Jump to: navigation, searchGeauga County,Information(EC-LEDS)Et1957) | Open2008) | OpenAl.,

58

Isotope Geothermometry At Lightning Dock Geothermal Area (Witcher, 2006) |  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data CenterFranconia, Virginia: Energy Resources Jump to:46 - 429 Throttled (botOpen Energy Information 2006) Jump to: navigation, search

59

Isotopic Analysis- Fluid At Lightning Dock Geothermal Area (Witcher, 2006)  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data CenterFranconia, Virginia: Energy Resources Jump to:46 - 429 Throttled (botOpen Energy2005) | Open Energy Information(1990) | OpenAl.,|

60

Cation Geothermometers At Lightning Dock Geothermal Area (Witcher, 2006) |  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home5b9fcbce19 NoPublic Utilities Address: 160Benin:Energy Information on PV Economics By BuildingCategory: Wind,000

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


61

Geothermal Literature Review At Lightning Dock Geothermal Area (Clemons, Et  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home5b9fcbce19 No revision hasInformation Earth's Heat Jump to: navigation, search OpenEIOpen Energy Information RanalliAl.,

62

Observation Wells At Lightning Dock Geothermal Area (Reeder, 1957) | Open  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home5b9fcbce19 No revision hasInformation Earth's HeatMexico:CommunityNorthwest BasinOahu, Hawaii:EnergyOpen Energy1988)

63

Thermal Gradient Holes At Lightning Dock Geothermal Area (Arnold...  

Open Energy Info (EERE)

DOE-funding Unknown Exploration Basis Known shallow hot spot in Animas Valley Notes Four thermal gradient holes were authorized to be drilled by AMEX, but no results were...

64

DockingShop: A Tool for Interactive Molecular Docking  

SciTech Connect (OSTI)

Given two independently determined molecular structures, the molecular docking problem predicts the bound association, or best fit between them, while allowing for conformational changes of the individual molecules during construction of a molecular complex. Docking Shop is an integrated environment that permits interactive molecular docking by navigating a ligand or protein to an estimated binding site of a receptor with real-time graphical feedback of scoring factors as visual guides. Our program can be used to create initial configurations for a protein docking prediction process. Its output--the structure of aprotein-ligand or protein-protein complex--may serve as an input for aprotein docking algorithm, or an optimization process. This tool provides molecular graphics interfaces for structure modeling, interactive manipulation, navigation, optimization, and dynamic visualization to aid users steer the prediction process using their biological knowledge.

Lu, Ting-Cheng; Max, Nelson L.; Ding, Jinhui; Bethel, E. Wes; Crivelli, Silvia N.

2005-04-24T23:59:59.000Z

65

5, 1129511329, 2005 Estimating lightning  

E-Print Network [OSTI]

of Mexico S. Beirle1 , N. Spichtinger2 , A. Stohl3 , K. L. Cummins4 , T. Turner4 , D. Boccippio5 , O. R vective system over the Gulf of Mexico. Simultaneously, cloud-to-ground (CG) flashes are counted by the U lightning15 activity. The enhancements can not be explained by transport of anthropogenic NOx and must

Boyer, Edmond

66

Changes in measured lightning return stroke peak current after the 1994 National Lightning Detection Network upgrade  

E-Print Network [OSTI]

Since a comprehensive upgrade of the US National Lightning Detection Network (NLDN) in 1994, the mean peak current of detected cloud-to-ground (CG) lightning flashes has decreased, the number of detected flashes has increased, and the percentage...

Wacker, Robert Scott

1997-01-01T23:59:59.000Z

67

Computational methods for molecular docking  

SciTech Connect (OSTI)

This tutorial was one of eight tutorials selected to be presented at the Third International Conference on Intelligent Systems for Molecular Biology which was held in the United Kingdom from July 16 to 19, 1995. Recently, it has been demonstrated that the knowledge of the three-dimensional structure of the protein can be used to derive new protein ligands with improved binding properties. This tutorial focuses on the following questions: What is its binding affinity toward a particular receptor? What are putative conformations of a ligand at the binding site? What are the similarities of different ligands in terms of their recognition capabilities? Where and in which orientation will a ligand bind to the active site? How is a new putative protein ligand selected? An overview is presented of the algorithms which are presently used to handle and predict protein-ligand interactions and to dock small molecule ligands into proteins.

Klebe, G. [BASF AG, Ludwigshafen (Germany); Lengauer, T.

1995-12-31T23:59:59.000Z

68

Total lightning characteristics of ordinary convection  

E-Print Network [OSTI]

descend and as they fall below the -10? C level they are electrified positively (snow crystals negative) due to riming electrification. This results in a tripole structure of charge. (from Takahashi 1984)??????????????????? 20 5... the unknowns involved in thunderstorm electrification and lightning may help alleviate the risk of lightning related fatalities, aviation mishaps, fires, and other lightning related dangers. ______________ This thesis follows the style of Atmospheric...

Motley, Shane Michael

2009-06-02T23:59:59.000Z

69

GWIDD: Genome-wide protein docking database  

E-Print Network [OSTI]

Structural information on interacting proteins is important for understanding life processes at the molecular level. Genome-wide docking database is an integrated resource for structural studies of proteinĖprotein interactions on the genome scale...

Kundrotas, Petras J.; Zhu, Zhengwei; Vasker, Ilya A.

2009-11-09T23:59:59.000Z

70

DNA  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

September 15, 2014 Computational analysis key to structural understanding of molecular machine that targets viral DNA LOS ALAMOS, N.M., Sept. 15, 2014-When this week's print issue...

71

Chapter 14 -Severe Weather Lightning Protection for the Farm  

E-Print Network [OSTI]

of farm fires. A well-installed and maintained lightning protection system routes lightning along a known of the electric power lines. Properly designed lightning arrestors should be placed between the power circuit and ground where the circuit enters the building. Large trees need protection from lightning. In addition

72

Numerical Modeling At Lightning Dock Geothermal Area (O'Brien, Et Al.,  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data CenterFranconia, Virginia: Energy ResourcesLoading map...(Utility Company)ReferencesNuiqsut, Alaska: Energy Resources JumpEnergy2013)1984)

73

Observation Wells At Lightning Dock Area (Warpinski, Et Al., 2004) | Open  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data CenterFranconia, Virginia: Energy ResourcesLoading map...(Utility Company)ReferencesNuiqsut,Place,Oakmont,Obion County, Tennessee:Energy

74

Ground Gravity Survey At Lightning Dock Area (Cunniff & Bowers, 2005) |  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data CenterFranconia, Virginia: Energy Resources Jump to: navigation,Ohio:Greer County is a county inAl., 1979) | Open EnergyOpen EnergyOpen

75

Thermal Gradient Holes At Lightning Dock Area (Cunniff & Bowers, 2005) |  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home5b9fcbce19 No revisionEnvReviewNonInvasiveExplorationUT-gTaguspark JumpDetective Jump to:theEnergyEnergyOpen Energy

76

Thermal Gradient Holes At Lightning Dock Geothermal Area (Arnold, Et Al.,  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home5b9fcbce19 No revisionEnvReviewNonInvasiveExplorationUT-gTaguspark JumpDetective Jump to:theEnergyEnergyOpen Energy1978) | Open

77

Thermal Gradient Holes At Lightning Dock Geothermal Area (Cunniff & Bowers,  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home5b9fcbce19 No revisionEnvReviewNonInvasiveExplorationUT-gTaguspark JumpDetective Jump to:theEnergyEnergyOpen Energy1978) |

78

Thermal Gradient Holes At Lightning Dock Geothermal Area (Cunniff, Et Al.,  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home5b9fcbce19 No revisionEnvReviewNonInvasiveExplorationUT-gTaguspark JumpDetective Jump to:theEnergyEnergyOpen Energy1978) |2003)

79

Stepout-Deepening Wells At Lightning Dock Area (Warpinski, Et Al., 2004) |  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home5b9fcbce19 No revisionEnvReviewNonInvasiveExplorationUT-g GrantAtlas (PACA

80

Surface Gas Sampling At Lightning Dock Area (Norman & Moore, 2004) | Open  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home5b9fcbce19 No revisionEnvReviewNonInvasiveExplorationUT-g GrantAtlas (PACAOpenSummerside WindSolar EnergySuperiorSureSurfEnergy

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


81

Surface Gas Sampling At Lightning Dock Area (Norman, Et Al., 2002) | Open  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home5b9fcbce19 No revisionEnvReviewNonInvasiveExplorationUT-g GrantAtlas (PACAOpenSummerside WindSolar

82

Compound and Elemental Analysis At Lightning Dock Area (Norman & Moore,  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Office of InspectorConcentratingRenewable Solutions LLC Jump to: navigation, search(Thompson, 1985) | Open Energy2004)

83

Production Wells At Lightning Dock Geothermal Area (McCants, 1974) | Open  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home5b9fcbce19 No revision hasInformation Earth'sOklahoma/GeothermalOrangePeru:JobInformationInformationOpen

84

Radiometrics At Lightning Dock Geothermal Area (Deal, Et Al., 1978) | Open  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home5b9fcbce19 No revisionEnvReviewNonInvasiveExplorationUT-g Grant of Access Permit5-ID-aREC Solar JumpRGSRadiant

85

Reflection Survey At Lightning Dock Area (Cunniff & Bowers, 2005) | Open  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home5b9fcbce19 No revisionEnvReviewNonInvasiveExplorationUT-g Grant of Access Permit5-ID-aRECRaton,RFPs| OpenJemez Pueblo Area

86

Gamma Log At Lightning Dock Geothermal Area (Witcher, 2006) | Open Energy  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data CenterFranconia, Virginia: Energy Resources Jump to: navigation, search Equivalent URIFrontier,JumpGahanna,Galveston BayInformation 6)

87

Gas Flux Sampling At Lightning Dock Area (Cunniff & Bowers, 2005) | Open  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data CenterFranconia, Virginia: Energy Resources Jump to: navigation, search Equivalent6894093¬į Loading69. ItLewicki &|Energy

88

Geology and geothermal waters of Lightning Dock region, Animas Valley and  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data CenterFranconia, Virginia: Energy Resources Jump to: navigation, searchGeauga County, Ohio: EnergySector:2008)the

89

Direct-Current Resistivity Survey At Lightning Dock Area (Warpinski, Et  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home5b9fcbce19 No revision has beenFfe2fb55-352f-473b-a2dd-50ae8b27f0a6 No revision has Type TermOpen EnergyEnergy2002) ||1979)

90

Aeromagnetic Survey At Lightning Dock Area (Warpinski, Et Al., 2002) | Open  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home5b9fcbce19 NoPublic Utilities Address: 160 East 300 SouthWaterBrasil Jump to:IowaResource(Nannini, 1986)

91

Analytical Modeling At Lightning Dock Geothermal Area (Brook, Et Al., 1978)  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home5b9fcbce19 NoPublic Utilities Address: 160 East 300Algoil JumpAltergyExperiments | OpenThe Tomoves Active| Open Energy

92

Flow Test At Lightning Dock Area (Cunniff & Bowers, 2005) | Open Energy  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home5b9fcbce19 No revision has beenFfe2fb55-352f-473b-a2dd-50ae8b27f0a6Theoretical vsFlint Geothermal Area (DOE GTP)

93

Fluid Inclusion Analysis At Lightning Dock Area (Norman & Moore, 2004) |  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home5b9fcbce19 No revision has beenFfe2fb55-352f-473b-a2dd-50ae8b27f0a6Theoretical vsFlint GeothermalInformation (1990)Open

94

Ground Gravity Survey At Lightning Dock Area (Warpinski, Et Al., 2002) |  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home5b9fcbce19 No revision hasInformation Earth's Heat Jump to:Photon Place:Net Jump to: navigation,2004) | Open EnergyOpen

95

Surface Gas Sampling At Lightning Dock Area (Norman & Moore, 2004) | Open  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data CenterFranconia, Virginia:FAQ < RAPID Jump to:Seadov Pty LtdSteen, Minnesota:36052¬į,Sunfield,FarmsSupport Resources, Inc1983)Energy

96

Surface Gas Sampling At Lightning Dock Area (Norman, Et Al., 2002) | Open  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data CenterFranconia, Virginia:FAQ < RAPID Jump to:Seadov Pty LtdSteen, Minnesota:36052¬į,Sunfield,FarmsSupport Resources,

97

Thermal Gradient Holes At Lightning Dock Area (Warpinski, Et Al., 2004) |  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data CenterFranconia, Virginia:FAQ < RAPID Jump to:Seadov Pty LtdSteen,Ltd Jump Jump to:InformationTheInformationAl.,Open Energy|Open

98

Thermal Ion Dispersion At Lightning Dock Area (Cunniff & Bowers, 2005) |  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data CenterFranconia, Virginia:FAQ < RAPID Jump to:Seadov Pty LtdSteen,Ltd Jump JumpAl., 1978) | Open Energy Information Date 19782004)

99

Crusader Automated Docking System Phase 3 report  

SciTech Connect (OSTI)

The US Army is developing the next generation of battlefield artillery vehicles, including an advanced, self-propelled howitzer and a companion resupply vehicle. The resupply vehicle is intended to rendezvous with the howitzer near the battlefront and to upload ammunition to the howitzer. The Army has recommended that the vehicles incorporate robotics to increase safety, by allowing the crew to remain inside their vehicles during resupply operations. Oak Ridge National Laboratory has developed an autonomous docking system for a 6-D.F. robotic, ammunition transfer arm. The docking system augments the operator`s abilities by determining the position and orientation (pose) of a docking port. The pose is the location of the x, y, and z reference axes in 3-D space; and the orientation is the rotations--roll, pitch, and yaw--about those axes. Bye precisely determining the pose of the docking port, the robot can be instructed to move to the docking position without operator intervention. The system uses a video camera and frame grabber to digitize images of the special docking port. Custom algorithms were developed to recognize the port in the camera image, to determine the pose from its image features, and to distribute the results to the robot control computer. The system is loosely coupled to the robot and can be easily adapted to different mechanical configurations. The system has successfully demonstrated autonomous docking on a 24-in. tabletop robot and a 12-ft ammunition resupply robot. The update rate, measurement accuracy, continuous operation, and accuracy with obstructed view have been determined experimentally.

Jatko, W.B.; Goddard, J.S.; Ferrell, R.K.; Gleason, S.S.; Hicks, J.S.; Varma, V.K.

1996-03-01T23:59:59.000Z

100

A comparative analysis of total lightning observations and cloud-to-ground lightning observations in the Southeastern United States region  

E-Print Network [OSTI]

Flashes of April 20, 1996, Orbit 13 After Performing Collocation Procedure. 19 21 5 OTD Versus NLDN Lightning Flashes. 24 6 OTD and NLDN Lightning Flashes of December 20, 1995, Orbit 2. 26 7 OTD and NLDN Lightning Flashes of July 23, 1996, Orbit 2... prototype for the Lightning Imaging Sensor launched aboard the Tropical Rainfall Measuring Mission (TRMM) [Goodman et aL, 1996], has enabled the detection of total lightning from space and allowed interesting comparisons of data coincident with ground...

Hugo, Keith Michael

1998-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


101

RDOCK: Refinement of Rigid-body Protein Docking Predictions  

E-Print Network [OSTI]

RDOCK: Refinement of Rigid-body Protein Docking Predictions Li Li,1 Rong Chen,1 and Zhiping Weng1 RDOCK for refining unbound predictions generated by a rigid-body docking algorithm ZDOCK, which has been, RDOCK represents the simplest approach toward refining unbound docking predictions. Despite its simplic

Weng, Zhiping

102

VRDD: Applying virtual reality visualization to protein docking  

E-Print Network [OSTI]

the levels of atomic overlap and interac- tion energy. The stunning VR graphics immerse users in the scene. Keywords: virtual reality, computer graphics, CAVE, mo- lecular docking, binding free energy calculations molecular docking aided by automatic docking and side chain confor- mational search. Binding free energies

Weng, Zhiping

103

Lightning protection system for a wind turbine  

DOE Patents [OSTI]

In a wind turbine (104, 500, 704) having a plurality of blades (132, 404, 516, 744) and a blade rotor hub (120, 712), a lightning protection system (100, 504, 700) for conducting lightning strikes to any one of the blades and the region surrounding the blade hub along a path around the blade hub and critical components of the wind turbine, such as the generator (112, 716), gearbox (708) and main turbine bearings (176, 724).

Costin, Daniel P. (Chelsea, VT); Petter, Jeffrey K. (Williston, VT)

2008-05-27T23:59:59.000Z

104

Lightning Arrestor Connectors Production Readiness  

SciTech Connect (OSTI)

The Lightning Arrestor Connector (LAC), part ďMĒ, presented opportunities to improve the processes used to fabricate LACs. The A## LACs were the first production LACs produced at the KCP, after the product was transferred from Pinnellas. The new LAC relied on the lessons learned from the A## LACs; however, additional improvements were needed to meet the required budget, yield, and schedule requirements. Improvement projects completed since 2001 include Hermetic Connector Sealing Improvement, Contact Assembly molding Improvement, development of a second vendor for LAC shells, general process improvement, tooling improvement, reduction of the LAC production cycle time, and documention of the LAC granule fabrication process. This report summarizes the accomplishments achieved in improving the LAC Production Readiness.

Marten, Steve; Linder, Kim; Emmons, Jim; Gomez, Antonio; Hasam, Dawud; Maurer, Michelle

2008-10-20T23:59:59.000Z

105

Analysis of charge transport during lightning using balloon-borne electric field sensors and Lightning  

E-Print Network [OSTI]

Analysis of charge transport during lightning using balloon-borne electric field sensors is developed to separate the background field associated with instrument rotation and cloud charging processes the background electric field that would have existed if the lightning had not occurred. The estimated background

Hager, William

106

Static electricity measurements for lightning warnings -an exploration  

E-Print Network [OSTI]

Static electricity measurements for lightning warnings - an exploration H. Bloemink De Bilt, 2013 | Internal report; IR 2013-01 #12;#12;Static electricity measurements for lightning warnings - an exploration Version 1.0 Date January 2013 Status Final #12;#12;Static electricity measurements for lightning warnings

Haak, Hein

107

Insights into the ground attachment process of natural lightning gained from an unusual triggered-lightning stroke  

E-Print Network [OSTI]

-lightning stroke J. Jerauld,1 M. A. Uman,1 V. A. Rakov,1 K. J. Rambo,1 and G. H. Schnetzer1 Received 19 June 2006 and magnetic field and current derivatives from an unusual triggered-lightning stroke that was initiated observed in the currents and in the distant radiation fields of natural-lightning first strokes. Field

Florida, University of

108

Augmenting Satellite Precipitation Estimation with Lightning Information  

SciTech Connect (OSTI)

We have used lightning information to augment the Precipitation Estimation from Remotely Sensed Imagery using an Artificial Neural Network - Cloud Classification System (PERSIANN-CCS). Co-located lightning data are used to segregate cloud patches, segmented from GOES-12 infrared data, into either electrified (EL) or non-electrified (NEL) patches. A set of features is extracted separately for the EL and NEL cloud patches. The features for the EL cloud patches include new features based on the lightning information. The cloud patches are classified and clustered using self-organizing maps (SOM). Then brightness temperature and rain rate (T-R) relationships are derived for the different clusters. Rain rates are estimated for the cloud patches based on their representative T-R relationship. The Equitable Threat Score (ETS) for daily precipitation estimates is improved by almost 12% for the winter season. In the summer, no significant improvements in ETS are noted.

Mahrooghy, Majid [Mississippi State University (MSU); Anantharaj, Valentine G [ORNL; Younan, Nicolas H. [Mississippi State University (MSU); Petersen, Walter A. [NASA Marshall Space Flight Center, Huntsville, AL; Hsu, Kuo-Lin [University of California, Irvine; Behrangi, Ali [Jet Propulsion Laboratory, Pasadena, CA; Aanstoos, James [Mississippi State University (MSU)

2013-01-01T23:59:59.000Z

109

Thunderstorm and Lightning Studies using the FORTE Optical Lightning System (FORTE/OLS)  

SciTech Connect (OSTI)

Preliminary observations of simultaneous RF and optical emissions from lightning as seen by the FORTE spacecraft are presented. RF/optical pairs of waveforms are routinely collected both as individual lightning events and as sequences of events associated with cloud-to-ground (CG) and intra-cloud (IC) flashes. CG pulses can be distinguished from IC pulses based on the properties of the RF and optical waveforms, but mostly based on the associated RF spectrograms. The RF spectrograms are very similar to previous ground-based VHF observations of lightning and show signatures associated with return strokes, stepped and dart leaders, and attachment processes,. RF emissions are observed to precede the arrival of optical emissions at the satellite by a mean value of 280 microseconds. The dual phenomenology nature of these observations are discussed in terms of their ability to contribute to a satellite-based lightning monitoring mission.

Argo, P.; Franz, R.; Green, J.; Guillen, J.L.; Jacobson, A.R.; Kirkland, M.; Knox, S.; Spalding, R.; Suszcynsky, D.M.

1999-02-01T23:59:59.000Z

110

Lightning location characteristics and vertical structure analysis of isolated storm cells in the TOGA COARE region  

E-Print Network [OSTI]

producing storm cells, and non-lightning producing cells over the tropical oceans. The characteristics of lightning locations with respect to radar reflectivity were also examined. Lightning characteristics of flashes associated with TOGA COARE storm cells...

Barnaby, Stephen Andrew

1995-01-01T23:59:59.000Z

111

E-Print Network 3.0 - analyzing lightning field Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

lightning field Search Powered by Explorit Topic List Advanced Search Sample search results for: analyzing lightning field Page: << < 1 2 3 4 5 > >> 1 Annales Geophysicae (2002)...

112

Meteorological characteristics associated with warm-season positive lightning events  

E-Print Network [OSTI]

. . . . . . . . . 60 29 Percent-positive values vs. Showalter Index for pre-storm soundings, with the outlier in Figure 28 removed. . 61 30 Percent-positive values vs. Total Totals Index for pre-storm soundings. . . . . . 64 31 Percent-positive values vs. Total... lightning and others primarily positive. Percent-positive values also vary greatly by season, with winter thunderstorms exhibiting much more positive lightning (Orville and Silver 1997). Positive lightning was not known to exist until 60 years ago, when...

Heggen, Paul Michael

2012-06-07T23:59:59.000Z

113

Structure of conducting channel of lightning  

SciTech Connect (OSTI)

The spatial distribution of the plasma density in a lightning channel is studied theoretically. It is shown that the electric-field double layer is formed at the channel boundary. In this case, the electron temperature changes abruptly and ions are accelerated by the electric field of the double layer. The ion momentum flux density is close to the surrounding gas pressure. Cleaning of the channel from heavy particles occurs in particle-exchange processes between the plasma channel and the surrounding air. Hydrogen ions are accumulated inside the expanding channel from the surrounding air, which is enriched by hydrogen-contained molecules. In this case, the plasma channel is unstable and splits to a chain of equidistant bunches of plasma. The hydrogen-enrich bunches burn diffusely after recombination exhibiting the bead lightning behavior.

Alanakyan, Yu. R. [Department of General and Applied Physics, Moscow Institute of Physics and Technology (State University), Dolgoprudny, 141700 Moscow Region (Russian Federation)] [Department of General and Applied Physics, Moscow Institute of Physics and Technology (State University), Dolgoprudny, 141700 Moscow Region (Russian Federation)

2013-08-15T23:59:59.000Z

114

Autonomous Docking of a Smart Wheelchair for the  

E-Print Network [OSTI]

utility vehicle. At the core of ATRS is a "smart" wheelchair sys- tem that autonomously navigates between tank, fuel system, and heating/ cooling systems of the vehicle (Wood, 2004). While enabling independent∑ ∑ ∑ ∑ ∑ ∑ ∑ ∑ ∑ ∑ ∑ ∑ ∑ ∑ ∑ ∑ ∑ ∑ ∑ ∑ ∑ ∑ ∑ ∑ ∑ ∑ ∑ ∑ ∑ ∑ ∑ Autonomous Docking of a Smart

Spletzer, John R.

115

Electromagnetic models of the lightning return stroke Yoshihiro Baba1  

E-Print Network [OSTI]

Electromagnetic models of the lightning return stroke Yoshihiro Baba1 and Vladimir A. Rakov2] Lightning return-stroke models are needed for specifying the source in studying the production of transient-called engineering models, electromagnetic return-stroke models allow a self-consistent full-wave solution for both

Florida, University of

116

LIGHTNING EXPOSURE OF WIND TURBINES University of Toronto  

E-Print Network [OSTI]

LIGHTNING EXPOSURE OF WIND TURBINES Dale Dolan University of Toronto e-mail: dale@ecf.utoronto.ca Abstract This paper applies the electrogeometric model of lightning exposure to a wind turbine to compute. For a typical 45 m wind turbine, the probability of being struck by a downward negative flash, as predicted

Lehn, Peter W.

117

-561 -105Pl LIGHTNING ELECTRIC AND MAGNETIC FIELDS  

E-Print Network [OSTI]

-scale pulse structure of naturally-occurring negative ground discharges, as observed at ground, includes in the valida- tion of lightning models. Table 1 summarizes essentially all identifiable lightning radiation field signatures as recorded at ground. Note that apparently there is no characteristic radiation pulse

Florida, University of

118

Demonstration Dock Designed to Benefit Eelgrass Habitat Restoration (Washington)  

SciTech Connect (OSTI)

The derelict Thomas Oil Dock on state tidelands in Port Townsend, Washington, was redesigned for the Northwest Maritime Center by a committee including marine scientists, architects, engineers, educators, regulators, and user groups. The committee's objectives were to create a ''demonstration dock'' using the best-available technologies and design features to restore nearshore habitat functions, particularly for threatened fisheries resources, while accommodating the unique requirements of the educational facility to house vessels ranging from historic tall ships to sea kayaks. A key nearshore habitat restoration goal was to reduce fragmentation of eelgrass where shade from the existing dock inhibited growth, by improving conditions with a new dock design and transplanting eelgrass to connect existing patches. Ecological conditions were evaluated through diver surveys; mapping existing eelgrass, macroalgae, and substrates; and review of controlling factors. Data on the attenuation an d diffusion of photosynthetically-active radiation (PAR) was collected and evaluated relative to eelgrass light requirements. Potential shade impacts of existing and alternative dock designs and materials were modeled. Key design features to improve habitat functions included the installation of reflective material under the dock to increase the incidence of PAR at the substrate level, reduction in the number of piles and associated shade impacts through the use of steel piles instead of wood, increase in the length of the trestle to 286 to move the greatest area of overwater structure beyond the range of eelgrass, and the use of grating in strategic locations to reduce the potential of a light/dark barrier to fish passage.

Diefenderfer, Heida L.; Sargeant, Susan L.; Thom, Ronald M.; Borde, Amy B.; Gayaldo, Perry F.; Curtis, Craig A.; Court, Brian L.; Pierce, David M.; Robison, David S.

2004-06-01T23:59:59.000Z

119

Regulatory guidance for lightning protection in nuclear power plants  

SciTech Connect (OSTI)

Oak Ridge National Laboratory (ORNL) was engaged by the U.S. Nuclear Regulatory Commission (NRC) Office of Nuclear Regulatory Research (RES) to develop the technical basis for regulatory guidance to address design and implementation practices for lightning protection systems in nuclear power plants (NPPs). Lightning protection is becoming increasingly important with the advent of digital and low-voltage analog systems in NPPs. These systems have the potential to be more vulnerable than older analog systems to the resulting power surges and electromagnetic interference (EMI) when lightning strikes facilities or power lines. This paper discusses the technical basis for guidance to licensees and applicants covered in Regulatory Guide (RG) 1.204, Guidelines for Lightning Protection of Nuclear Power Plants, issued August 2005. RG 1.204 describes guidance for practices that are acceptable to the NRC staff for protecting nuclear power structures and systems from direct lightning strikes and the resulting secondary effects. (authors)

Kisner, R. A.; Wilgen, J. B.; Ewing, P. D.; Korsah, K. [Oak Ridge National Laboratory, P. O. Box 2008, Oak Ridge, TN 37831-6007 (United States); Antonescu, C. E. [U.S. Nuclear Regulatory Commission, Washington, DC 20555 (United States)

2006-07-01T23:59:59.000Z

120

Regulatory Guidance for Lightning Protection in Nuclear Power Plants  

SciTech Connect (OSTI)

Abstract - Oak Ridge National Laboratory (ORNL) was engaged by the U.S. Nuclear Regulatory Commission (NRC) Office of Nuclear Regulatory Research (RES) to develop the technical basis for regulatory guidance to address design and implementation practices for lightning protection systems in nuclear power plants (NPPs). Lightning protection is becoming increasingly important with the advent of digital and low-voltage analog systems in NPPs. These systems have the potential to be more vulnerable than older analog systems to the resulting power surges and electromagnetic interference (EMI) when lightning strikes facilities or power lines. This paper discusses the technical basis for guidance to licensees and applicants covered in Regulatory Guide (RG) 1.204, Guidelines for Lightning Protection of Nuclear Power Plants, issued August 2005. RG 1.204 describes guidance for practices that are acceptable to the NRC staff for protecting nuclear power structures and systems from direct lightning strikes and the resulting secondary effects.

Kisner, Roger A [ORNL; Wilgen, John B [ORNL; Ewing, Paul D [ORNL; Korsah, Kofi [ORNL; Antonescu, Christina E [ORNL

2006-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


121

GRED STUDIES AND DRILLING OF AMERICULTURE STATE 2, AMERICULTURE TILAPIA FARM LIGHTNING DOCK KGRA, ANIMAS VALLEY, NM  

SciTech Connect (OSTI)

This report summarizes the GRED drilling operations in the AmeriCulture State 2 well with an overview of the preliminary geologic and geothermal findings, from drill cuttings, core, geophysical logs and water geochemical sampling.

Witcher, James

2006-08-01T23:59:59.000Z

122

Lightning strokes can probe the ionosphere  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr May JunDatastreamsmmcrcalgovInstrumentsruc DocumentationP-SeriesFlickrinformationPostdocsCenterCenter (LMI-EFRC) -Principles andLightning

123

Lightning and radar observations of hurricane Rita landfall  

SciTech Connect (OSTI)

Los Alamos National Laboratory (LANL) owns and operates an array of Very-Low Frequency (VLF) sensors that measure the Radio-Frequency (RF) waveforms emitted by Cloud-to-Ground (CG) and InCloud (IC) lightning. This array, the Los Alamos Sferic Array (LASA), has approximately 15 sensors concentrated in the Great Plains and Florida, which detect electric field changes in a bandwidth from 200 Hz to 500 kHz (Smith et al., 2002). Recently, LANL has begun development of a new dual-band RF sensor array that includes the Very-High Frequency (VHF) band as well as the VLF. Whereas VLF lightning emissions can be used to deduce physical parameters such as lightning type and peak current, VHF emissions can be used to perform precise 3d mapping of individual radiation sources, which can number in the thousands for a typical CG flash. These new dual-band sensors will be used to monitor lightning activity in hurricanes in an effort to better predict intensification cycles. Although the new LANL dual-band array is not yet operational, we have begun initial work utilizing both VLF and VHF lightning data to monitor hurricane evolution. In this paper, we present the temporal evolution of Rita's landfall using VLF and VHF lightning data, and also WSR-88D radar. At landfall, Rita's northern eyewall experienced strong updrafts and significant lightning activity that appear to mark a transition between oceanic hurricane dynamics and continental thunderstorm dynamics. In section 2, we give a brief overview of Hurricane Rita, including its development as a hurricane and its lightning history. In the following section, we present WSR-88D data of Rita's landfall, including reflectivity images and temporal variation. In section 4, we present both VHF and VLF lightning data, overplotted on radar reflectivity images. Finally, we discuss our observations, including a comparison to previous studies and a brief conclusion.

Henderson, Bradley G [Los Alamos National Laboratory; Suszcynsky, David M [Los Alamos National Laboratory; Hamlin, Timothy E [Los Alamos National Laboratory; Jeffery, C A [Los Alamos National Laboratory; Wiens, Kyle C [TEXAS TECH U.; Orville, R E [TEXAS A& M

2009-01-01T23:59:59.000Z

124

Experts are partnering in Tampa, the Lightning Capital of the U.S., to provide safety information about the dangers of lightning and promote  

E-Print Network [OSTI]

this free event to learn more about the national Lightning Safety Awareness Campaign to protect your family about the dangers of lightning and promote risk reduction and lightning protection education. Attend safety & protection resources FEATURED GUESTS INCLUDE: · Tampa Mayor Bob Buckhorn · Tampa Fire Chief

125

UPGRADE AND EVALUATION OF A LIGHTNING DETECTION SYSTEM  

E-Print Network [OSTI]

/11/2004 page 3 of 17 1 INTRODUCTION KNMI (Royal Netherlands Meteorological Institute) has upgraded itsUPGRADE AND EVALUATION OF A LIGHTNING DETECTION SYSTEM Hans Beekhuis Iwan Holleman the Netherlands

Stoffelen, Ad

126

The search for Titan lightning radio emissions G. Fischer1  

E-Print Network [OSTI]

was caused by lightning exciting a Schumann resonance in the ionospheric cavity. In a further investigation instability mechanism. Morente et al. [2008] claimed the extraction of six Schumann resonances in agreement

Gurnett, Donald A.

127

Automated Docking of Highly Flexible Ligands by Genetic Algorithms: A Critical Assessment  

E-Print Network [OSTI]

condition for successful docking. © 2003 Wiley Periodicals, Inc. J Comput Chem 25: 412­422, 2004 Key words

Caflisch, Amedeo

128

Enlightening lightning! Producing and directing a multimedia planetarium show  

E-Print Network [OSTI]

is middle school aged children. The goal of the show is to teach lightning safety and lightning facts in an immersive environment. Through the use of video, an animated character, and a meteorologist, the curriculum is presented to the audience. I...! by starting with outlining the curriculum and finishing with putting it all together at the planetarium. The goal of this paper is to discuss the techniques and organizational methods used to manage a diverse group and produce a multimedia show. iv...

Fowler, Sarah Marie

2005-02-17T23:59:59.000Z

129

DARS (Decoys As the Reference State) Potentials for Protein-Protein Docking  

E-Print Network [OSTI]

DARS (Decoys As the Reference State) Potentials for Protein-Protein Docking Gwo-Yu Chuang,* Dima As the Reference State (DARS) is a simple and natural approach to the construction of structure directly in docking calculations. We investigated the performance of various DARS versions for docking

Vajda, Sandor

130

An Integrated Docking Pipeline for the Prediction of Large-Scale Protein-Protein Interactions  

E-Print Network [OSTI]

An Integrated Docking Pipeline for the Prediction of Large-Scale Protein-Protein Interactions Xin. In this study, we developed a protein-protein docking pipeline (PPDP) that integrates a variety of state studies. In this study, we developed a protein-protein docking pipeline by integrat

131

X-ray Emission from Thunderstorms and Lightning  

ScienceCinema (OSTI)

How lightning is initiated in the relatively low electric fields inside thunderclouds and how it can then propagate for tens of kilometers through virgin air are two of the great unsolved problems in the atmospheric sciences.† Until very recently it was believed that lightning was entirely a conventional discharge, involving only low-energy (a few eV) electrons.† This picture changed completely a few years ago with the discovery of intense x-ray emission from both natural cloud-to-ground lightning and rocket-triggered lightning.† This energetic emission cannot be produced by a conventional discharge, and so the presence of x-rays strongly implies that runaway breakdown plays a role in lightning processes.† During runaway breakdown, electrons are accelerated through air to nearly the speed of light by strong electric fields.† These runaway electrons then emit bremsstrahlung x-rays and gamma-rays during collisions with air.† Indeed, the x-ray and gamma-ray emission produced by runaway breakdown near the tops of thunderstorms is bright enough to be seen from outer space, 600 km away.† As a result, the physics used for decades to describe thunderstorm electrification and lightning discharges is incomplete and needs to be revisited.†

Joseph Dwyer

2010-01-08T23:59:59.000Z

132

Reliability of lightning resistant overhead distribution lines  

SciTech Connect (OSTI)

An assessment of the 32 year historical reliability of the 13.8 kV electrical distribution system at the Oak Ridge National Laboratory (ORNL) in Tennessee has yielded several conclusions useful In the planning of Industrial power Systems. The system configuration at ORNL has essentially remained unchanged in the last 32 years which allows a meaningful comparison of reliability trends for the plant`s eight overhead distribution lines, two of which were built in the 1960`s with lightning resistant construction techniques. Meticulous records indicating the cause, duration, and location of 135 electric outages in the plant`s distribution system have allowed a reliability assessment to be performed. The assessment clearly shows how differences in voltage construction class, length, age, and maximum elevation above a reference elevation influence the reliability of overhead power distribution lines. Comparisons are also made between the ORNL historical data and predicted failure rates from ANSI and IEEE industry surveys.

Tolbert, L.M.; Cleveland, J.T.; Degenhardt, L.J.

1995-04-01T23:59:59.000Z

133

DOCKING METHODS, LIGAND DESIGN, AND VALIDATING DATA SETS IN THE  

E-Print Network [OSTI]

27 DOCKING METHODS, LIGAND DESIGN, AND VALIDATING DATA SETS IN THE STRUCTURAL GENOMICS ERA Natasja and identify the active component in extracts from natural products that were used. A number of developments in the equilibrium toward the best-fitting conformers for both, resulting in binding (Foote and Milstein, 1994

Bourne, Philip E.

134

Severe convection and lightning in subtropical South America By Kristen L. Rasmussen1  

E-Print Network [OSTI]

1 Severe convection and lightning in subtropical South America of the seasonal, diurnal, and extreme storm-related lightning in South America. · Preference for hail radar and radiometer data show that subtropical South America has the world's deepest convective

Houze Jr., Robert A.

135

Physical Modeling of the Motions of a Container Ship Moored to a Dock with Comparison to Numerical Simulation  

E-Print Network [OSTI]

ship moored to a dock, a 1:50 scale model is moored to two types of dock, solid wall dock and pile supported dock. Irregular waves of TMA spectrum with various periods, heights, and directions are generated in the wave basin to induce the motions...

Zhi, Yuanzhe

2013-07-11T23:59:59.000Z

136

Data Assimilation of Lightning in WRF 3/4-D VAR Using Observation Operators  

E-Print Network [OSTI]

@fsu.edu Abstract Compared to other types of satellite-derived data, assimilating lightning data into operationalData Assimilation of Lightning in WRF 3/4-D VAR Using Observation Operators Razvan S¬łtefanescu1. The early stages of our research will utilize existing ground-based lightning data that can be assimilated

Navon, Michael

137

Strong electric fields from positive lightning strokes in the stratosphere R. H. Holzworth,1  

E-Print Network [OSTI]

Strong electric fields from positive lightning strokes in the stratosphere R. H. Holzworth,1 M. P] A balloon payload launched in Brazil has measured vector electric fields from lightning at least an order of the electric field transient propagation. These measurements imply that lightning electric fields

Thomas, Jeremy N.

138

A possible relationship between Global Warming and Lightning Activity in India during the period 1998-2009  

E-Print Network [OSTI]

Lightning activity on a global scale has been studied season wise using satellite data for the period from 1998 to 2009. Lightning activity shows an increasing trend during the period of study which is highly correlated with atmospheric warming. A similar increasing trend of lightning activity is observed in the Indian region during the pre-monsoon season which is correlated with global lightning trends and warming trends of surface temperature in India. Key words: Global warming, lightning activity, Solar cycle changes

Felix Pereira B.; Priyadarsini G.; T. E. Girish

2010-12-15T23:59:59.000Z

139

E-Print Network 3.0 - antibody-antigen docking compensates Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

SnugDock: Paratope ... Source: Gray, Jeffrey J. - Department of Biomolecular and Chemical Engineering, Johns Hopkins University Collection: Chemistry ; Biology and Medicine 2...

140

Total lightning observations of severe convection over North Texas  

E-Print Network [OSTI]

Density GSD Gridded Source Density LDAR Lightning Detection and Ranging MCS Mesoscale Convective System MSI Mesocyclone Strength Index MxFED Maximum Flash Extent Density MxFIDT Maximum Flash Initiation Density Total MxGSD Maximum Gridded Source.......................................................................................... 1 1.2 Background ....................................................................................... 4 1.3 Thesis Objectives and Hypothesis...................................................... 19 2. DATA AND METHODOLOGY...

McKinney, Christopher Michael

2009-05-15T23:59:59.000Z

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


141

High-energy radiation from thunderstorms and lightning with LOFT  

E-Print Network [OSTI]

This is a White Paper in support of the mission concept of the Large Observatory for X-ray Timing (LOFT), proposed as a medium-sized ESA mission. We discuss the potential of LOFT for the study of high-energy radiation from thunderstorms and lightning. For a summary, we refer to the paper.

Marisaldi, M; Brandt, S; Briggs, M S; Budtz-JÝrgensen, C; Campana, R; Carlson, B E; Celestin, S; Connaughton, V; Cummer, S A; Dwyer, J R; Fishman, G J; Fullekrug, M; Fuschino, F; Gjesteland, T; Neubert, T; ōstgaard, N; Tavani, M

2015-01-01T23:59:59.000Z

142

Carbon dioxide emission during forest fires ignited by lightning  

E-Print Network [OSTI]

In this paper we developed the model for the carbon dioxide emission from forest fire. The master equation for the spreading of the carbon dioxide to atmosphere is the hyperbolic diffusion equation. In the paper we study forest fire ignited by lightning. In that case the fores fire has the well defined front which propagates with finite velocity.

Magdalena Pelc; Radoslaw Osuch

2009-03-31T23:59:59.000Z

143

Update Direct-Strike Lightning Environment for Stockpile-to-Target Sequence: Supplement LLNL Subcontract #B568621 Lightning Protection at the Yucca Mountain Waste Storage Facility  

SciTech Connect (OSTI)

The University of Florida has surveyed all relevant publications reporting lightning damage to metals, metals which could be used as components of storage containers for nuclear waste materials. We show that even the most severe lightning could not penetrate the stainless steel thicknesses proposed for nuclear waste storage casks.

Uman, M A

2008-10-09T23:59:59.000Z

144

Yucca: An Efficient Algorithm for Small-Molecule Docking1 by Vicky Choi  

E-Print Network [OSTI]

docking algorithms have been developed (see reviews [1][2] and comparison studies [3 ¬Ī 5]). Broadly and Genome Informatics√?. #12;algorithms are based on genetic algorithms and/or Monte Carlo-simulated, multi-conformer docking algorithms separately generate a set of low-energy conformers, and then do rigid

Choi, Vicky

145

Computer Graphics in Real-time Docking with Energy Calculation and Minimization  

E-Print Network [OSTI]

Computer Graphics in Real-time Docking with Energy Calculation and Minimization N. Pattabiraman, M. Levitt*, T.E. Ferrin, R. Langridge Computer Graphics Laboratory, Department of Pharmaceutical Chemistry 1985 We describe a real-time docking method using molecular graphics and high-speed calculation

Levitt, Michael

146

Thunderstorm lightning and radar characteristics: insights on electrification and severe weather forecasting  

E-Print Network [OSTI]

thunderstorm?s dynamics and electrification processes operate. The advent of the WSR- 88D nationwide (U. S.) radar system, the National Lightning Detection Network (NLDN), and 3-dimensional lightning mapping systems (here the Lightning Detection... that are related to storm intensity and can assist in predicting severe weather. 2. Data and methodology The radar data used in this study were from the Dallas-Fort Worth WSR-88D (KFWS), obtained from the National Climatic Data Center...

Steiger, Scott Michael

2007-04-25T23:59:59.000Z

147

Waste Isolation Pilot Plant TruDock crane system analysis  

SciTech Connect (OSTI)

The WIPP TruDock crane system located in the Waste Handling Building was identified in the WIPP Safety Analysis Report (SAR), November 1995, as a potential accident concern due to failures which could result in a dropped load. The objective of this analysis is to evaluate the frequency of failure of the TruDock crane system resulting in a dropped load and subsequent loss of primary containment, i.e. drum failure. The frequency of dropped loads was estimated to be 9.81E-03/year or approximately one every 102 years (or, for the 25% contingency, 7.36E-03/year or approximately one every 136 years). The dominant accident contributor was the failure of the cable/hook assemblies, based on failure data obtained from NUREG-0612, as analyzed by PLG, Inc. The WIPP crane system undergoes a rigorous test and maintenance program, crane operation is discontinued following any abnormality, and the crane operator and load spotter are required to be trained in safe crane operation, therefore it is felt that the WIPP crane performance will exceed the data presented in NUREG-0612 and the estimated failure frequency is felt to be conservative.

Morris, B.C. [Westinghouse Electric Corp., Pittsburgh, PA (United States); Carter, M. [Westinghouse Electric Corp., Carlsbad, NM (United States). Waste Isolation Div.

1996-10-01T23:59:59.000Z

148

Lightning sferics and stroke-delayed pulses measured in the stratosphere: Implications for mesospheric currents  

E-Print Network [OSTI]

Lightning sferics and stroke-delayed pulses measured in the stratosphere: Implications-to-ground (CG) lightning strokes at horizontal distances of 75­600 km were measured at altitudes of 30­35 km. Electric field changes were measured for each of the 2467 CG strokes detected by the Brazilian Integrated

Thomas, Jeremy N.

149

Leader/return-stroke-like processes in the initial stage of rocket-triggered lightning  

E-Print Network [OSTI]

Leader/return-stroke-like processes in the initial stage of rocket-triggered lightning R. C. Olsen, the process of current cutoff and reestablishment during the initial stage is similar to a leader/return-stroke) than those in a triggered or natural lightning subsequent stroke (10­15 kA). The events were separated

Florida, University of

150

A stepped leader model for lightning including charge distribution in branched channels  

SciTech Connect (OSTI)

The stepped leader process in negative cloud-to-ground lightning plays a vital role in lightning protection analysis. As lightning discharge usually presents significant branched or tortuous channels, the charge distribution along the branched channels and the stochastic feature of stepped leader propagation were investigated in this paper. The charge density along the leader channel and the charge in the leader tip for each lightning branch were approximated by introducing branch correlation coefficients. In combination with geometric characteristics of natural lightning discharge, a stochastic stepped leader propagation model was presented based on the fractal theory. By comparing simulation results with the statistics of natural lightning discharges, it was found that the fractal dimension of lightning trajectory in simulation was in the range of that observed in nature and the calculation results of electric field at ground level were in good agreement with the measurements of a negative flash, which shows the validity of this proposed model. Furthermore, a new equation to estimate the lightning striking distance to flat ground was suggested based on the present model. The striking distance obtained by this new equation is smaller than the value estimated by previous equations, which indicates that the traditional equations may somewhat overestimate the attractive effect of the ground.

Shi, Wei; Zhang, Li [School of Electrical Engineering, Shandong University, Jinan 250061 (China); Li, Qingmin, E-mail: lqmeee@ncepu.edu.cn [Beijing Key Lab of HV and EMC, North China Electric Power University, Beijing 102206 (China); State Key Lab of Alternate Electrical Power System with Renewable Energy Sources, Beijing 102206 (China)

2014-09-14T23:59:59.000Z

151

Source altitudes of terrestrial gamma-ray flashes produced by lightning leaders  

E-Print Network [OSTI]

; published 18 April 2012. [1] Terrestrial gamma-ray flashes (TGFs) are energetic photon bursts observed fromSource altitudes of terrestrial gamma-ray flashes produced by lightning leaders Wei Xu,1 Sebastien. Pasko (2012), Source altitudes of terres- trial gamma-ray flashes produced by lightning leaders, Geophys

Pasko, Victor

152

A ground level gamma-ray burst observed in association with rocket-triggered lightning  

E-Print Network [OSTI]

A ground level gamma-ray burst observed in association with rocket-triggered lightning J. R. Dwyer 2004; published 13 March 2004. [1] We report the observation of an intense gamma-ray burst observed lightning channel with gamma-ray energies extending up to more than 10 MeV. The burst consisted of 227

Florida, University of

153

Nanotechnology with DNA DNA Nanodevices  

E-Print Network [OSTI]

Nanotechnology with DNA DNA Nanodevices Friedrich C. Simmel* and Wendy U. Dittmer A DNA actuator. Introduction.............285 2. Overview: DNA Nanotechnology.......285 3. Prototypes of Nanomechanical DNA overview of DNA nanotechnology as a whole is given. The most important properties of DNA molecules

Ludwig-Maximilians-Universit√§t, M√ľnchen

154

Update Direct-Strike Lightning Environment for Stockpile-to-Target Sequence  

SciTech Connect (OSTI)

The University of Florida has surveyed all relevant publications reporting lightning characteristics and presents here an up-to-date version of the direct-strike lightning environment specifications for nuclear weapons published in 1989 by R. J. Fisher and M. A. Uman. Further, we present functional expressions for current vs. time, current derivative vs. time, second current derivative vs. time, charge transfer vs. time, and action integral (specific energy) vs. time for first return strokes, for subsequent return strokes, and for continuing currents; and we give sets of constants for these expressions so that they yield approximately the median and extreme negative lightning parameters presented in this report. Expressions for the median negative lightning waveforms are plotted. Finally, we provide information on direct-strike lightning damage to metals such as stainless steel, which could be used as components of storage containers for nuclear waste materials; and we describe UF's new experimental research program to add to the sparse data base on the properties of positive lightning. Our literature survey, referred to above, is included in four Appendices. The following four sections (II, III, IV, and V) of this final report deal with related aspects of the research: Section II. Recommended Direct-Strike Median and Extreme Parameters; Section III. Time-Domain Waveforms for First Strokes, Subsequent Strokes, and Continuing Currents; Section IV. Damage to Metal Surfaces by Lightning Currents; and Section V. Measurement of the Characteristics of Positive Lightning. Results of the literature search used to derive the material in Section II and Section IV are found in the Appendices: Appendix 1. Return Stroke Current, Appendix 2. Continuing Current, Appendix 3. Positive Lightning, and Appendix 4. Lightning Damage to Metal Surfaces.

Uman, M A; Rakov, V A; Elisme, J O; Jordan, D M; Biagi, C J; Hill, J D

2008-10-01T23:59:59.000Z

155

Evaluation of an approximate method for incorporating floating docks in harbor wave prediction models  

E-Print Network [OSTI]

coastal domains. However, floating structures such as floating breakwaters and docks are often encountered in the modeling domain. This makes the problem locally 3- dimensional. Hence it is problematic to incorporate a floating structure into the 2-d model...

Tang, Zhaoxiang

2005-11-01T23:59:59.000Z

156

E-Print Network 3.0 - arm docking complex Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

more complex, Summary: with four degrees of freedom (shown in Figure 6(a)), an AdeptOne robot arm (depicted as the docking vehicle... the needed information to control the arm...

157

Protein Docking by the Interface Structure Similarity: How Much Structure Is Needed?  

E-Print Network [OSTI]

The increasing availability of co-crystallized protein-protein complexes provides an opportunity to use template-based modeling for protein-protein docking. Structure alignment techniques are useful in detection of remote ...

Sinha, Rohita; Kundrotas, Petras J.; Vakser, Ilya A.

2012-02-13T23:59:59.000Z

158

Optimal location of cross-docking centers for a distribution network in Argentina  

E-Print Network [OSTI]

The objective of this thesis is to define an optimal distribution network for Argentina using Cross Docking Centers. The products to be delivered are in a Central Warehouse in Buenos Aires, the main city and port of ...

Dobrusky, Fernando G. (Fernando Gabriel), 1971-

2003-01-01T23:59:59.000Z

159

A radar study of the interaction between lightning and precipitation  

SciTech Connect (OSTI)

A radar study was made of the interaction between lightning and precipitation with the 430 MHz Doppler radar at the Arecibo Observatory in Puerto Rico. On one occasion, the spectral power at Doppler velocities near that corresponding to the updraft increased substantially within a fraction of a second after a discharge was detected in the beam. Calculations were made to simulate the effect of an electric field change on mean Doppler velocity for a distribution of droplets in a thunderstorm. 13 refs., 4 figs.

Holden, D.N.; Ulbrich, C.W.

1988-01-01T23:59:59.000Z

160

Crusader Automated Docking System: Technology support for the Crusader Resupply Team. Interim report, Ammunition Logistics Program  

SciTech Connect (OSTI)

The US Army and Team Crusader (United Defense, Lockheed Martin Armament Systems, etc.) are developing the next generation howitzer, the Crusader. The development program includes an advanced, self-propelled liquid propellant howitzer and a companion resupply vehicle. The resupply vehicle is intended to rendezvous with the howitzer near the battlefront and replenish ammunition, fuel, and other material. The Army has recommended that Crusader incorporate new and innovative technologies to improve performance and safety. One conceptual design proposes a robotic resupply boom on the resupply vehicle to upload supplies to the howitzer. The resupply boom would normally be retracted inside the resupply vehicle during transit. When the two vehicles are within range of the resupply boom, the boom would be extended to a receiving port on the howitzer. In order to reduce exposure to small arms fire or nuclear, biological, and chemical hazards, the crew would remain inside the resupply vehicle during the resupply operation. The process of extending the boom and linking with the receiving port is called docking. A boom operator would be designated to maneuver the boom into contact with the receiving port using a mechanical joystick. The docking operation depends greatly upon the skill of the boom operator to manipulate the boom into docking position. Computer simulations at the National Aeronautics and Space Administration have shown that computer-assisted or autonomous docking can improve the ability of the operator to dock safely and quickly. This document describes the present status of the Crusader Autonomous Docking System (CADS) implemented at Oak Ridge National laboratory (ORNL). The purpose of the CADS project is to determine the feasibility and performance limitations of vision systems to satisfy the autonomous docking requirements for Crusader and conduct a demonstration under controlled conditions.

Kring, C.T.; Varma, V.K.; Jatko, W.B.

1995-11-01T23:59:59.000Z

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


161

Probability, consequences, and mitigation for lightning strikes of Hanford high level waste tanks  

SciTech Connect (OSTI)

The purpose of this report is to summarize selected lightning issues concerning the Hanford Waste Tanks. These issues include the probability of a lightning discharge striking the area immediately adjacent to a tank including a riser, the consequences of significant energy deposition from a lightning strike in a tank, and mitigating actions that have been or are being taken. The major conclusion of this report is that the probability of a lightning strike deposition sufficient energy in a tank to cause an effect on employees or the public is unlikely;but there are insufficient, quantitative data on the tanks and waste to prove that. Protection, such as grounding of risers and air terminals on existing light poles, is recommended.

Zach, J.J.

1996-06-05T23:59:59.000Z

162

Probability, consequences, and mitigation for lightning strikes to Hanford site high-level waste tanks  

SciTech Connect (OSTI)

The purpose of this report is to summarize selected lightning issues concerning the Hanford Waste Tanks. These issues include the probability of lightning discharge striking the area immediately adjacent to a tank including a riser, the consequences of significant energy deposition from a lightning strike in a tank, and mitigating actions that have been or are being taken. The major conclusion of this report is that the probability of a lightning strike depositing sufficient energy in a tank to cause an effect on employees or the public is unlikely;but there are insufficient, quantitative data on the tanks and waste to prove that. Protection, such as grounding of risers and air terminals on existing light poles, is recommended.

Zach, J.J.

1996-08-01T23:59:59.000Z

163

The warning time for cloud-to-ground lightning in isolated, ordinary thunderstorms over Houston, Texas  

E-Print Network [OSTI]

electrical circuit. The thunderstorm is efectively a batery, charging the earth?s surface with negative charge lowered due to lightning discharges. Altitudes are not to scale........................................................8 3 Positive dipole... circuit. The thunderstorm is efectively a batery, charging the earth?s surface with negative charge lowered due to lightning discharges. Altitudes are not to scale (From MacGorman and Rust 1998, p. 31). 9 To determine...

Clements, Nathan Chase

2009-05-15T23:59:59.000Z

164

Cloud-to-ground lightning-precipitation relationships in the South Central United States  

E-Print Network [OSTI]

, none of which has been conclusively proven (Dong and Hallett 1992). A discussion of the full physics is beyond the scope of this review, however, some important observations are necessary. One explanation for the charge separation mechanism... ~ ' + (i) Figure 2. Schematic of a typical lightning discharge. See text for explauadou. (Adapted from M. A. Uman, Understanding Lightning, BEK Technical Pub. , pp. 74-75, 78-79. ) (Figure 2f). The stepped leader and the upward discharge meet...

Sheridan, Scott Christopher

2012-06-07T23:59:59.000Z

165

Docking HPr to HPr Kinase with Partial Backbone Flexibility A thesis submitted to the Johns Hopkins University in conformity with the  

E-Print Network [OSTI]

Docking HPr to HPr Kinase with Partial Backbone Flexibility By Lian Guo A thesis submitted Assessment of Prediction of Interactions, a blind test of protein docking) challenge. Here, a method

Gray, Jeffrey J.

166

A New Chemoinformatics-Based Approach to Protein Docking: Fourier Correlation of PCA-Selected Probe Potentials  

E-Print Network [OSTI]

of the principal difficulties in computational macromolecular docking is in devising a reliable energy by a docking search. However, there is no direct way to infer which of the several types of intermolecular Component Analysis (PCA) of Molecular Interaction Fields (MIFs) to help select the most significant types

Ritchie, Dave

167

A climatology, synoptic assessment, and thermodynamic evaluation for cloud-to-ground lightning in Georgia: a study for the 1996 Summer Olympics  

E-Print Network [OSTI]

A lightning climatology within 50 km of nine outdoor venue locations for the 1996 Summer Olympics is produced. Spatial and temporal patterns were analyzed for July and August from 1986 to 1993. Active and inactive lightning days were isolated...

Livingston, Eric Scott

1995-01-01T23:59:59.000Z

168

PIPER: An FFT-Based Protein Docking Program with Pairwise Potentials  

E-Print Network [OSTI]

intermolecular potentials. The DARS (Decoys As the Reference State) potentials are extracted from structures distributions in the reference state. The current version of the DARS potential works well for enzyme≠inhibitor complexes. With the new FFT-based program, DARS provides much better docking results than the earlier

Vajda, Sandor

169

IEEE TRANSACTIONS ON ELECTROMAGNETIC COMPATIBILITY, VOL. 51, NO. 3, AUGUST 2009 471 New Insights Into Lightning Return-Stroke Models  

E-Print Network [OSTI]

Into Lightning Return-Stroke Models With Specified Longitudinal Current Distribution Grzegorz Maslowski, Member, IEEE, and Vladimir A. Rakov, Fellow, IEEE (Invited Paper) Abstract--We show that any lightning return-stroke-current-source-type) models, it is also unipolar but directed into the channel core. The conversion between LCS and DCS return-stroke

Florida, University of

170

Close electric field signatures of dart leader//return stroke sequences in rocket-triggered lightning showing residual fields  

E-Print Network [OSTI]

to 30 m deposited by the leader but presumably left unneutralized by the return stroke. This residual-triggered lightning showing residual fields V. A. Rakov and V. Kodali Department of Electrical and Computer and 30 m from the negative lightning channel are used to examine the so-called residual electric field

Florida, University of

171

P1.15 A GIS-Based Approach to Lightning Studies for West Texas and New Mexico  

E-Print Network [OSTI]

quality controlled. These quality control processes included removing weak positive flashes (those less quality controlled, the domain was divided into 2.5 km x 2.5 km gridboxes. Lightning flashes within each box then were counted and plotted using ArcGIS to produce a total lightning image consisting of all

Fuelberg, Henry

172

1D+4D-VAR data assimilation of lightning with WRFDA system using nonlinear observation operators  

E-Print Network [OSTI]

1 1D+4D-VAR data assimilation of lightning with WRFDA system using nonlinear observation operators of assimilating data from the Earth Networks Total Lightning Network (ENTLN) during two cases of severe weather Mapper (GLM). We use the Weather Research and Forecast (WRF) model and variational data assimilation

Navon, Michael

173

Protection characteristics of a Faraday cage compromised by lightning burnthrough.  

SciTech Connect (OSTI)

A lightning flash consists of multiple, high-amplitude but short duration return strokes. Between the return strokes is a lower amplitude, continuing current which flows for longer duration. If the walls of a Faraday cage are made of thin enough metal, the continuing current can melt a hole through the metal in a process called burnthrough. A subsequent return stroke can couple energy through this newly-formed hole. This LDRD is a study of the protection provided by a Faraday cage when it has been compromised by burnthrough. We initially repeated some previous experiments and expanded on them in terms of scope and diagnostics to form a knowledge baseline of the coupling phenomena. We then used a combination of experiment, analysis and numerical modeling to study four coupling mechanisms: indirect electric field coupling, indirect magnetic field coupling, conduction through plasma and breakdown through the hole. We discovered voltages higher than those encountered in the previous set of experiments (on the order of several hundreds of volts).

Warne, Larry Kevin; Bystrom, Edward; Jorgenson, Roy Eberhardt; Montoya, Sandra L.; Merewether, Kimball O.; Coats, Rebecca Sue; Martinez, Leonard E.; Jojola, John M.

2012-01-01T23:59:59.000Z

174

DNA demethylation by DNA repair  

E-Print Network [OSTI]

Active DNA demethylation underlies key facets of reproduction in flowering plants and mammals and serves a general genome housekeeping function in plants. A family of 5-methylcytosine DNA glycosylases catalyzes plant ...

Gehring, Mary

175

Development of a guidance, navigation and control architecture and validation process enabling autonomous docking to a tumbling satellite  

E-Print Network [OSTI]

The capability to routinely perform autonomous docking is a key enabling technology for future space exploration, as well as assembly and servicing missions for spacecraft and commercial satellites. Particularly, in more ...

Nolet, Simon, 1975-

2007-01-01T23:59:59.000Z

176

DECK: Distance and environment-dependent, coarse-grained, knowledge-based potentials for protein-protein docking  

E-Print Network [OSTI]

Background: Computational approaches to protein-protein docking typically include scoring aimed at improving the rank of the near-native structure relative to the false-positive matches. Knowledge-based potentials improve ...

Liu, Shiyong; Vakser, Ilya A.

2011-07-11T23:59:59.000Z

177

Comparing Evolutionary Programs and Evolutionary Pattern Search Algorithms: A Drug Docking Application  

SciTech Connect (OSTI)

Evolutionary programs (EPs) and evolutionary pattern search algorithms (EPSAS) are two general classes of evolutionary methods for optimizing on continuous domains. The relative performance of these methods has been evaluated on standard global optimization test functions, and these results suggest that EPSAs more robustly converge to near-optimal solutions than EPs. In this paper we evaluate the relative performance of EPSAs and EPs on a real-world application: flexible ligand binding in the Autodock docking software. We compare the performance of these methods on a suite of docking test problems. Our results confirm that EPSAs and EPs have comparable performance, and they suggest that EPSAs may be more robust on larger, more complex problems.

Hart, W.E.

1999-02-10T23:59:59.000Z

178

Binding hotspots of BAZ2B bromodomain: histone interaction revealed by solution NMR driven docking  

E-Print Network [OSTI]

, Dow Street, Dundee, DD1 5EH, UK Corresponding Author * To whom correspondence should be addressed: Alessio Ciulli, Phone: +44 1382 386230, Fax: +44 1382 386373, Email: a.ciulli@dundee.ac.uk Funding Source Statement: This work was supported... spectroscopy and docking simulations. The resulting models were validated with site-directed mutagenesis and peptide binding studies using ITC. EXPERIMENTAL PROCEDURES Protein expression Escherichia coli Rosetta competent cells were transformed with a p...

Ferguson, Fleur M.; Dias, David M.; Rodrigues, Joao P. G. L. M.; Wienk, Hans; Bonvin, Alexandre M. J. J.; Abell, Chris; Ciulli, Alessio

2014-09-30T23:59:59.000Z

179

A MODIFIED TRANSMISSION LINE MODEL FOR LIGHTNING RETURN STROKE FIELD CALCULATIONS  

E-Print Network [OSTI]

- 229 - 44Hl A MODIFIED TRANSMISSION LINE MODEL FOR LIGHTNING RETURN STROKE FIELD CALCULATIONS V. The modifications considered differ from each other by the height dependent attenuation factor for the return stroke data available. Some problems with modeling of the early and late stages of the return stroke process

Florida, University of

180

Simultaneous radio and satellite optical measurements of high-altitude sprite current and lightning continuing current  

E-Print Network [OSTI]

continuing current Steven A. Cummer,1 Harald U. Frey,2 Stephen B. Mende,2 Rue-Ron Hsu,3 Han-Tzong Su,3 Alfred [Fu¨llekrug and Constable, 2000; Sato and Fukunishi, 2003], and the measurement of lightning charge

California at Berkeley, University of

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


181

Lightning strikes to tall objects: Currents inferred from far electromagnetic fields versus directly measured currents  

E-Print Network [OSTI]

-field-to-current conversion factor for lightning strikes to flat ground based on the transmission line model and (b currents on tall towers are used for testing the validity of field-to-current conversion equations [e on current measurement location (e.g., near the top or bottom of the tower) and on whether initial or largest

Florida, University of

182

A lightning summary and decision model for thunderstorm prediction at Whiteman Air Force Base, Missouri  

E-Print Network [OSTI]

A cloud-to-ground lightning summary was developed for a 139xl85 kilometer area centered at Whiteman Air Force Base. Spatial and temporal patterns, and first stroke peak currents were analyzed from 1989-1995. Stability indices were examined...

Bass, Randall Gerald

1996-01-01T23:59:59.000Z

183

Rare measurements of a sprite with halo event driven by a negative lightning discharge over Argentina  

E-Print Network [OSTI]

Argentina M. J. Taylor,1 M. A. Bailey,1 P. D. Pautet,1 S. A. Cummer,2 N. Jaugey,2 J. N. Thomas,3,4 N. N measurements were made of a mesoscale thunderstorm observed on February 22­23, 2006 over northern Argentina of a sprite with halo event driven by a negative lightning discharge over Argentina, Geophys. Res. Lett., 35

Thomas, Jeremy N.

184

Environmental control of cloud-to-ground lightning polarity in severe storms  

E-Print Network [OSTI]

polarity of severe storms by directly affecting their structural, dynamical, and microphysical properties, which in turn directly control cloud electrification and CG flash polarity. A more specific hypothesis, which has been supported by past............................................................................... 23 a. Thunderstorm electrification ................................................ 23 1) Charging mechanisms and typical charge structure ... 23 2) Cloud-to-ground lightning flash................................. 27 3...

Buffalo, Kurt Matthew

2008-10-10T23:59:59.000Z

185

Measurements and implications of the relationship between lightning and terrestrial gamma ray flashes  

E-Print Network [OSTI]

the discovery of short bursts of gamma rays originating from Earth, called terrestrial gamma ray flashes (TGFsMeasurements and implications of the relationship between lightning and terrestrial gamma ray associated with 26 terrestrial gamma ray flashes (TGFs) recorded by the RHESSI satellite over the Caribbean

Cummer, Steven A.

186

A review of ten years of triggered-lightning experiments at Camp Blanding, Florida  

E-Print Network [OSTI]

of Florida, 533 Engineering Bldg. #33, P.O. Box 116130, Gainesville, FL 32611, United States Received 31 30 per year, with about 20 of them containing return-strokes. Out of a total of 208 flashes in TableA review of ten years of triggered-lightning experiments at Camp Blanding, Florida Vladimir A

Florida, University of

187

Seasonal variations of global lightning activity extracted from Schumann resonances using a genetic  

E-Print Network [OSTI]

Seasonal variations of global lightning activity extracted from Schumann resonances using a genetic of Schumann resonances (SR). Comparison of the results derived from our FDTD model and the previous studies by other authors on related subjects shows that Schumann resonance is a good probe to indicate the seasonal

Pasko, Victor

188

A study of lightning activity over the warm pool western Pacific Ocean (TOGA-COARE region) for 1993  

E-Print Network [OSTI]

The warm pool western Pacific Ocean is an area of the equatorial tropics characterized by strong and frequent convection, and vigorous lightning activity. However, it has been noted by various researchers that the vast oceanic expanses experience...

Rios, Luis Alberto

1995-01-01T23:59:59.000Z

189

Cloud-to-ground lightning characteristics of warm season Mesoscale Convection Systems in the Central United States: 1992-1993  

E-Print Network [OSTI]

This study provides a detailed analysis of cloud-to-aphics. ground (CG) lightning flashes within individual Mesoscale Convective Systems (MCSs) that occurred in the Central United States during May-August of 1992 and 1993. Analysis of the CG...

Hoeth, Brian Richard

2012-06-07T23:59:59.000Z

190

The evolution of total lightning and radar reflectivity characteristics of two mesoscale convective systems over Houston, Texas  

E-Print Network [OSTI]

originated in the convective or transition regions. Both in-situ charging mechanisms created by the development of the mesoscale updraft and charge advection by the front-to-rear flow likely contribute to the increased electrification and lightning...

Hodapp, Charles Lee

2009-05-15T23:59:59.000Z

191

IEEE I&CPS Conf., May 7-11, San Antonio, Texas, pp. 147-152 Reliability of Lightning Resistant  

E-Print Network [OSTI]

IEEE I&CPS Conf., May 7-11, San Antonio, Texas, pp. 147-152 Reliability of Lightning Resistant essentially remained unchanged in the last 32 years which allows a meaningful comparison of reliability trends

Tolbert, Leon M.

192

Investigation into the high percentage of positive CG lightning along the west coast of the United States  

E-Print Network [OSTI]

INVESTIGATION INTO THE HIGH PERCENTAGE OF POSITIVE CG LIGHTNING ALONG THE WEST COAST OF THE UNITED STATES A Thesis by BRANDON LEE ELY Submitted to the Office of Graduate Studies of Texas A&M University in partial fulfillment... of the requirements for the degree of MASTER OF SCIENCE December 2002 Major Subject: Atmospheric Sciences INVESTIGATION INTO THK HIGH PERCENTAGE OF POSITIVE CG LIGHTNING ALONG THE WEST COAST OF THK UNITED STATES A Thesis by BRANDON LEE ELY Submitted to Texas...

Ely, Brandon Lee

2012-06-07T23:59:59.000Z

193

New Revelation of Lightning Ball Observation and Proposal for a Nuclear Reactor Fusion Experiment  

E-Print Network [OSTI]

In this paper, the author brings further details regarding his Lightning Ball observation that were not mentioned in the first one (Ref.1-2). Additionally, he goes more into detail as the three forces that are necessary to allow the residual crescent form the hydrodynamic vortex ring to shrink into a sphere.Further topics are the similarities and analogies between the Lightning Ball formation's theory and the presently undertaken Tokamak-Stellarator-Spheromak fusion reactor experiments. A new theory and its experimental realisation are proposed as to make the shrinking of the hot plasma of reactors into a ball possible by means of the so called long range electromagnetic forces. In this way,the fusion ignition temperature could possibly atteined.

Domokos Tar

2009-10-12T23:59:59.000Z

194

New Revelation of Lightning Ball Observation and Proposal for a Nuclear Reactor Fusion Experiment  

E-Print Network [OSTI]

In this paper, the author brings further details regarding his Lightning Ball observation that were not mentioned in the first one (Ref.1-2). Additionally, he goes more into detail as the three forces that are necessary to allow the residual crescent form the hydrodynamic vortex ring to shrink into a sphere.Further topics are the similarities and analogies between the Lightning Ball formation's theory and the presently undertaken Tokamak-Stellarator-Spheromak fusion reactor experiments. A new theory and its experimental realisation are proposed as to make the shrinking of the hot plasma of reactors into a ball possible by means of the so called long range electromagnetic forces. In this way,the fusion ignition temperature could possibly atteined.

Tar, Domokos

2009-01-01T23:59:59.000Z

195

Problem 65 in Section 4.1 (Page 274) Constructing a pipeline Supertankers off-load oil at a docking facility 4 mi offshore. The nearest refinery  

E-Print Network [OSTI]

facility 4 mi offshore. The nearest refinery is 9 mi east of the shore point nearest the docking facility. A pipeline must be constructed connecting the docking facility with the refinery. The pipeline costs $300.42 miles away from the refinery, or equivalently 3.58 miles away from Point A (as the back of the book has

Schilling, Anne

196

Experimental Measurements and Numerical Prediction of the Effect of Waves on Mooring Line Forces for a Container Ship Moored to Pile Supported and Solid Wall Docks  

E-Print Network [OSTI]

710 ft ship and model dock. The dock construction, solid sheet wall or pile supported, was varied to add another aspect of a moored vessel. Mooring lines were modeled using 14 springs in typical mooring line arrangement to simulate the elastic...

Luai, Andres B

2013-05-03T23:59:59.000Z

197

A Ball Lightning Model as a Possible Explanation of Recently Reported Cavity Lights  

SciTech Connect (OSTI)

The salient features of cavity lights, in particular, mobile luminous objects (MLO's), as have been experimentally observed in superconducting accelerator cavities, are summarized. A model based upon standard electromagnetic interactions between a small particle and the 1.5 GHz cavity excitation field is described. This model can explain some features of these data, in particular, the existence of particle orbits without wall contact. While this result is an important success for the model, it is detailed why the model as it stands is incomplete. It is argued that no avenues for a suitable extension of the model through established physics appear evident, which motivates an investigation of a model based upon a more exotic object, ball lightning. As discussed, further motivation derives from the fact that there are significant similarities in many of the qualitative features of ball lightning and MLO's, even though they appear in quite different circumstances and differ in scale by orders of magnitude. The ball lightning model, which incorporates electromagnetic charges and currents, is based on a symmetrized set of Maxwell's equations in which the electromagnetic sources and fields are characterized by a process called dyality rotation. It is shown that a consistent mathematical description of dyality rotation as a physical process can be achieved by adding suitable (phenomenological) current terms to supplement the usual current terms in the symmetrized Maxwell's equations. These currents, which enable the conservation of electric and magnetic charge, are called vacuum currents. It is shown that the proposed ball lightning model offers a good qualitative explanation of the perplexing aspects of the MLO data. Avenues for further study are indicated.

Fryberger, David; /SLAC

2009-08-04T23:59:59.000Z

198

Measurement and modeling of transfer functions for lightning coupling into the Sago mine.  

SciTech Connect (OSTI)

This report documents measurements and analytical modeling of electromagnetic transfer functions to quantify the ability of cloud-to-ground lightning strokes (including horizontal arc-channel components) to couple electromagnetic energy into the Sago mine located near Buckhannon, WV. Two coupling mechanisms were measured: direct and indirect drive. These transfer functions are then used to predict electric fields within the mine and induced voltages on conductors that were left abandoned in the sealed area of the Sago mine.

Morris, Marvin E.; Higgins, Matthew B.

2007-04-01T23:59:59.000Z

199

Thermodynamic Perspective on the Dock-Lock Growth Mechanism of Amyloid Fibrils Edward P. O'Brien,,  

E-Print Network [OSTI]

for Physical Science and Technology, Department of Chemistry and Biochemistry, UniVersity of Maryland, College, Japan, and Department of Chemistry, Boston UniVersity, Boston, Massachusetts 02215 ReceiVed: May 28 (docking) followed by a slower conformational rearrangement leading to the incorporation onto

Thirumalai, Devarajan

200

Identification of critical chemical features for Aurora kinase-B inhibitors using Hip-Hop, virtual screening and molecular docking  

E-Print Network [OSTI]

Identification of critical chemical features for Aurora kinase-B inhibitors using Hip-Hop, virtual November 2010 Keywords: Aurora kinase Pharmacophore Molecular docking Hip-Hop Homology modeling Molecular for Aurora kinase-B inhibitors using the potent methods like Hip-Hop, virtual screening, homology modeling

Lee, Keun Woo

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


201

498 IEEE JOURNAL OF OCEANIC ENGINEERING, VOL. 26, NO. 4, OCTOBER 2001 Docking for an Autonomous Ocean Sampling  

E-Print Network [OSTI]

498 IEEE JOURNAL OF OCEANIC ENGINEERING, VOL. 26, NO. 4, OCTOBER 2001 Docking for an Autonomous of Applied Ocean Physics and Engineering, Woods Hole Oceano- graphic Institution, Woods Hole, MA 02543 USA (e is with the Department of Ocean Engineering, Massachusetts Insti- tute of Technology, Cambridge, MA 02139 USA (e

Singh, Hanumant

202

A unified radiative magnetohydrodynamics code for lightning-like discharge simulations  

SciTech Connect (OSTI)

A two-dimensional Eulerian finite difference code is developed for solving the non-ideal magnetohydrodynamic (MHD) equations including the effects of self-consistent magnetic field, thermal conduction, resistivity, gravity, and radiation transfer, which when combined with specified pulse current models and plasma equations of state, can be used as a unified lightning return stroke solver. The differential equations are written in the covariant form in the cylindrical geometry and kept in the conservative form which enables some high-accuracy shock capturing schemes to be equipped in the lightning channel configuration naturally. In this code, the 5-order weighted essentially non-oscillatory scheme combined with Lax-Friedrichs flux splitting method is introduced for computing the convection terms of the MHD equations. The 3-order total variation diminishing Runge-Kutta integral operator is also equipped to keep the time-space accuracy of consistency. The numerical algorithms for non-ideal terms, e.g., artificial viscosity, resistivity, and thermal conduction, are introduced in the code via operator splitting method. This code assumes the radiation is in local thermodynamic equilibrium with plasma components and the flux limited diffusion algorithm with grey opacities is implemented for computing the radiation transfer. The transport coefficients and equation of state in this code are obtained from detailed particle population distribution calculation, which makes the numerical model is self-consistent. This code is systematically validated via the Sedov blast solutions and then used for lightning return stroke simulations with the peak current being 20?kA, 30?kA, and 40?kA, respectively. The results show that this numerical model consistent with observations and previous numerical results. The population distribution evolution and energy conservation problems are also discussed.

Chen, Qiang, E-mail: cq0405@126.com; Chen, Bin, E-mail: emcchen@163.com; Xiong, Run; Cai, Zhaoyang [National Key Laboratory of Electromagnetic Environment and Electro-optical Engineering, PLA University of Science and Technology, Nanjing 210007 (China)] [National Key Laboratory of Electromagnetic Environment and Electro-optical Engineering, PLA University of Science and Technology, Nanjing 210007 (China); Chen, P. F., E-mail: chenpf@nju.edu.cn [School of Astronomy and Space Science, Nanjing University, Nanjing 210093 (China)

2014-03-15T23:59:59.000Z

203

The convective structures associated with cloud-to-ground lightning in TOGA COARE Mesoscale Convective Systems  

E-Print Network [OSTI]

suggested that the threshold of about 40 dBZ at the -10 C level for rapid cloud electrification found in New Mexico by Dye et al. (1989) could be valid for tropical convection as well. Orville and Henderson (1986), and Goodman and Christian (1993), have... along with small ice and supercooled liquid water for cloud electrification and lightning to occur. Since most oceanic VPRR drop off rapidly above the freezing level compared to continental VPRR, this would provide evidence that the updraft velocities...

Restivo, Michael Edward

1995-01-01T23:59:59.000Z

204

Radar-Derived Forecasts of Cloud-to-Ground Lightning Over Houston, Texas  

E-Print Network [OSTI]

,399 unique cells, and 1,028,510 to find the best lightning forecast criteria. Results show that using 30 dBZ at the -20 ?C isotherm on cells within 75 km of the radar that have been tracked for at least 2 consecutive scan produces the best forecasts... with a critical success index (CSI) of 0.71. The best VII predictor was 0.734 kg m-2 on cells within 75 km of the radar that have been tracked for at least 2 consecutive scans iv producing a CSI of 0.68. Results of this study further suggest...

Mosier, Richard Matthew

2011-02-22T23:59:59.000Z

205

Measured voltages and currents internal to closed metal cylinders due to diffusion of simulated lightning currents  

SciTech Connect (OSTI)

One mechanism for the penetration of lightning energy into the interior of a weapon is by current diffusion through the exterior metal case. Tests were conducted in which simulated lightning currents were driven over the exteriors of similar aluminum and ferrous steel cylinders of 0.125-in wall thickness. Under conditions in which the test currents were driven asymmetrically over the exteriors of the cylinders, voltages were measured between various test points in the interior as functions of the amplitude and duration of the applied current. The maximum recorded open-circuit voltage, which occurred in the steel cylinder, was 1.7 V. On separate shots, currents flowing on a low impedance shorting conductor between the same set of test points were also measured, yielding a maximum current of 630 A, again occurring across the interior of the steel cylinder. Under symmetrical exterior drive current conditions, a maximum end-to-end internal voltage of 4.1 V was obtained, also in the steel cylinder, with a corresponding current of 480 A measured on a coaxial conductor connected between the two end plates of the cylinder. Data were acquired over a range of input current amplitudes between about 40 and 100 kA. These data provide the experimental basis for validating models that can subsequently be applied to real weapons and other objects of interest.

Schnetzer, G.H.; Fisher, R.J.

1994-08-01T23:59:59.000Z

206

Physical mechanism and numerical simulation of the inception of the lightning upward leader  

SciTech Connect (OSTI)

The upward leader is a key physical process of the leader progression model of lightning shielding. The inception mechanism and criterion of the upward leader need further understanding and clarification. Based on leader discharge theory, this paper proposes the critical electric field intensity of the stable upward leader (CEFISUL) and characterizes it by the valve electric field intensity on the conductor surface, E{sub L}, which is the basis of a new inception criterion for the upward leader. Through numerical simulation under various physical conditions, we verified that E{sub L} is mainly related to the conductor radius, and data fitting yields the mathematical expression of E{sub L}. We further establish a computational model for lightning shielding performance of the transmission lines based on the proposed CEFISUL criterion, which reproduces the shielding failure rate of typical UHV transmission lines. The model-based calculation results agree well with the statistical data from on-site operations, which show the effectiveness and validity of the CEFISUL criterion.

Li Qingmin [Beijing Key Lab of High Voltage and EMC, School of Electric and Electronic Engineering, North China Electric Power University, Beijing 102206 (China) and State Key Lab of Alternate Electrical Power System with Renewable Energy Sources, North China Electric Power University, Beijing 102206 (China); Lu Xinchang; Shi Wei; Zhang Li; Zou Liang; Lou Jie [Shandong Provincial Key Lab of UHV Technology and Gas Discharge, School of Electrical Engineering, Shandong University, Jinan 250061 (China)

2012-12-15T23:59:59.000Z

207

Observed one-dimensional return stroke propagation speeds in the bottom 170 m of a rocket-triggered lightning channel  

E-Print Network [OSTI]

Observed one-dimensional return stroke propagation speeds in the bottom 170 m of a rocket; revised 22 June 2004; accepted 8 July 2004; published 20 August 2004. [1] The return-stroke propagation speeds of five strokes from a seven-stroke triggered lightning flash are measured, with a 2 ns sampling

Florida, University of

208

First versus subsequent return-stroke current and field peaks in negative cloud-to-ground lightning discharges  

E-Print Network [OSTI]

First versus subsequent return-stroke current and field peaks in negative cloud-to-ground lightning examine relative magnitudes of electric field peaks of first and subsequent return strokes in negative, the electric field peak of the first stroke is appreciably, 1.7 to 2.4 times, larger than the field peak

Florida, University of

209

Electric and magnetic fields and field derivatives from lightning stepped leaders and first return strokes measured at distances  

E-Print Network [OSTI]

Electric and magnetic fields and field derivatives from lightning stepped leaders and first return; published 5 September 2008. [1] Using electric and magnetic field and field derivative sensors arrayed over-peak width of the stepped-leader/return-stroke electric field waveform; the stepped-leader electric field

Florida, University of

210

IEEE TRANSACTIONS ON ELECTROMAGNETIC COMPATIBILITY, VOL. 50, NO. 3, AUGUST 2008 651 Analysis of Lightning-Induced Voltages on  

E-Print Network [OSTI]

over lossy ground produced by lightning strikes either to flat ground or to a tall tower are calculated by return-stroke speed, and essentially inde- pendent of return-stroke model [transmission-line (TL), modified transmission line with linear current decay with height (MTLL), or modified transmission line

Florida, University of

211

Asiakastyytyvšisyystutkimus - DNA Kauppa Lappeenranta.  

E-Print Network [OSTI]

??OpinnšytetyŲn aiheena oli DNA Kauppa Lappeenrannan asiakastyytyvšisyyden tutkiminen. Tutkimuksen ensisijaisena tavoitteena oli selvittšš, kuinka tyytyvšisiš asiakkaat olivat olleet DNA Kauppa Lappeenrannan ja asiakkaan všliseen kontaktipintaanÖ (more)

Pitkšnen, Jesse

2013-01-01T23:59:59.000Z

212

AC and lightning performance of fiberglass crossarms aged in 115 kV transmission line  

SciTech Connect (OSTI)

This paper presents the results of an investigation of the electrical performance of 115 kV transmission line fiberglass cross-arm used by Mississippi Power and Light Company. A transmission line fiberglass crossarm removed from service and companion cross-arms outdoors but not in service were examined. The evaluation of electrical performance was based on flashover voltage value at AC voltage and standard lightning impulses as well as under dry and wet conditions. The tests were performed in the Mississippi State University High Voltage Laboratory. The obtained flashover voltages show no large differences in electrical strength of fiberglass crossarms removed from service and those stored outdoors. The Added CFO voltage by fiberglass crossarm to the porcelain suspension insulators is presented versus the length of the fiberglass crossarm for dry and wet conditions.

Grzybowski, S. (Mississippi State Univ., Mississippi State, MS (United States). Dept. of Electrical and Computer Engineering); Jenkins, E.B. (Entergy, New Orleans, LA (United States). Generation and Transmission Group)

1993-10-01T23:59:59.000Z

213

Comments to support the Dipole Dynamical Model (DDM) of Ball Lightning (BL)  

E-Print Network [OSTI]

I present estimates to justify previously proposed by me heuristic Dipole Dynamical Model (DDM) of Ball Lightning (BL). The movement and energy supplying to the dipole BL are due to the atmospheric electric field. Crucial for the detailed analysis of BL is using the new relation of balance of the force of atmospheric electric field (per unit mass of electron cloud) and dipole forces electrons-ions within BL dipole (per unit mass of BL) as the first necessary condition for the existance of BL as an integer. This model is unique because, unlike existing static models, fundamental condition for the existence of Ball Lightning is its forward motion. The virial theorem limiting BL power does not apply to BL which is not closed system like the Sun or Galaxy systems and is strongly dependent part of the infinitely extended in time and space large system. Stability of BL is due to two free parameters with the fundamental role of thermodynamic non-equilibrium, ionization, recombination and translational movement with energy loss by radiation and also excess volumetric positive charge. Polarization degree of BL plasma is characterized by polarizability factor {\\gamma}. An example is presented of calculating the stability of option BL. There is also a possible connection of stability BL with statistical distributions of the atmospheric electric field in time and space. Destruction of BL can also occur due to arising kinematical instability at its accelerating (or decelerating) movement. Maximal energy density in BL DDM does not exceed the value Espec<(10(8) - 10(9)) J/m(3). Resulting indefinitely long BL lifetime is also discussed.

V. N. Soshnikov

2015-02-01T23:59:59.000Z

214

Combined 3D-QSAR, molecular docking and molecular dynamics study on thyroid hormone activity of hydroxylated polybrominated diphenyl ethers to thyroid receptors ?  

SciTech Connect (OSTI)

Several recent reports suggested that hydroxylated polybrominated diphenyl ethers (HO-PBDEs) may disturb thyroid hormone homeostasis. To illuminate the structural features for thyroid hormone activity of HO-PBDEs and the binding mode between HO-PBDEs and thyroid hormone receptor (TR), the hormone activity of a series of HO-PBDEs to thyroid receptors ? was studied based on the combination of 3D-QSAR, molecular docking, and molecular dynamics (MD) methods. The ligand- and receptor-based 3D-QSAR models were obtained using Comparative Molecular Similarity Index Analysis (CoMSIA) method. The optimum CoMSIA model with region focusing yielded satisfactory statistical results: leave-one-out cross-validation correlation coefficient (q{sup 2}) was 0.571 and non-cross-validation correlation coefficient (r{sup 2}) was 0.951. Furthermore, the results of internal validation such as bootstrapping, leave-many-out cross-validation, and progressive scrambling as well as external validation indicated the rationality and good predictive ability of the best model. In addition, molecular docking elucidated the conformations of compounds and key amino acid residues at the docking pocket, MD simulation further determined the binding process and validated the rationality of docking results. -- Highlights: ? The thyroid hormone activities of HO-PBDEs were studied by 3D-QSAR. ? The binding modes between HO-PBDEs and TR? were explored. ? 3D-QSAR, molecular docking, and molecular dynamics (MD) methods were performed.

Li, Xiaolin [State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210046 (China)] [State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210046 (China); Ye, Li [Suzhou NeuPharma Co.,Ltd, Suzhou 215123 (China)] [Suzhou NeuPharma Co.,Ltd, Suzhou 215123 (China); Wang, Xiaoxiang [State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210046 (China)] [State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210046 (China); Wang, Xinzhou [Suzhou NeuPharma Co.,Ltd, Suzhou 215123 (China)] [Suzhou NeuPharma Co.,Ltd, Suzhou 215123 (China); Liu, Hongling [State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210046 (China)] [State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210046 (China); Zhu, Yongliang [Suzhou NeuPharma Co.,Ltd, Suzhou 215123 (China)] [Suzhou NeuPharma Co.,Ltd, Suzhou 215123 (China); Yu, Hongxia, E-mail: hongxiayu01@yahoo.com.cn [State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210046 (China)] [State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210046 (China)

2012-12-15T23:59:59.000Z

215

DNA Sequencing apparatus  

DOE Patents [OSTI]

An automated DNA sequencing apparatus having a reactor for providing at least two series of DNA products formed from a single primer and a DNA strand, each DNA product of a series differing in molecular weight and having a chain terminating agent at one end; separating means for separating the DNA products to form a series bands, the intensity of substantially all nearby bands in a different series being different, band reading means for determining the position an This invention was made with government support including a grant from the U.S. Public Health Service, contract number AI-06045. The U.S. government has certain rights in the invention.

Tabor, Stanley (Cambridge, MA); Richardson, Charles C. (Chestnut Hill, MA)

1992-01-01T23:59:59.000Z

216

Annual Report: 2011-2012 Storm Season Sampling, Non-Dry Dock Stormwater Monitoring for Puget Sound Naval Shipyard, Bremerton, WA  

SciTech Connect (OSTI)

Annual PSNS non-dry dock storm water monitoring results for 2011-2012 storm season. Included are a brief description of the sampling procedures, storm event information, laboratory methods and data collection, a results and discussion section, and the conclusions and recommendations.

Brandenberger, Jill M.; Metallo, David; Rupert, Brian; Johnston, Robert K.; Gebhart, Christine

2013-07-03T23:59:59.000Z

217

Detection and quantitation of single nucleotide polymorphisms, DNA sequence variations, DNA mutations, DNA damage and DNA mismatches  

SciTech Connect (OSTI)

DNA mutation binding proteins alone and as chimeric proteins with nucleases are used with solid supports to detect DNA sequence variations, DNA mutations and single nucleotide polymorphisms. The solid supports may be flow cytometry beads, DNA chips, glass slides or DNA dips sticks. DNA molecules are coupled to solid supports to form DNA-support complexes. Labeled DNA is used with unlabeled DNA mutation binding proteins such at TthMutS to detect DNA sequence variations, DNA mutations and single nucleotide length polymorphisms by binding which gives an increase in signal. Unlabeled DNA is utilized with labeled chimeras to detect DNA sequence variations, DNA mutations and single nucleotide length polymorphisms by nuclease activity of the chimera which gives a decrease in signal.

McCutchen-Maloney, Sandra L. (Pleasanton, CA)

2002-01-01T23:59:59.000Z

218

Relationships between tropical cyclone intensity and satellite based indicators of inner core convection: 85 GHz ice scattering signature and lightning  

E-Print Network [OSTI]

. REFERENCES. . . . . VITA. . . 116 119 125 LIST OF FIGURES FIGURE I Typhoon Oscar 85 GHz PCT iield at 2117 UTC 15 September 1995. . . . , Page 2 Correlations between 0-1' radius PCT parameters and hurricane/typhoon intensity at the time of SSM... miniinum PCT and lightning in Hurricane Barbara. . 79 46 Typhoon Oscar best track, 12 IJTC 7 September - 0 UTC 18 September 1995. . 81 47 Oscar 85 GHz PCT field at 2014 UTC 12 September 1995. . . . . . 48 Oscar 85 GHz PCT field at 2112 IJTC 13 September...

Cecil, Daniel Joseph

1997-01-01T23:59:59.000Z

219

A classification of Texas thunderstorms according to their cloud-to-ground lightning characteristics during spring 1993  

E-Print Network [OSTI]

OF FIGURES Figure Page 1 Study region, depicted by dotted area. 10 2 Study area DF sites with nominal 400 km range rings. . . . 10 3 Depiction of "inland" and "coastal" areas of the study region. . . . . . 16 Lightning plots associated with a cold ji... onral storm system (lefl) and a rrough storm system (right), separated by the heavy black line. . . . . . . . , . . . . . . . 19 Ground flash density contours in flashes km r for (a) April-June 1993, (b) April 1993, (c) May 1993, and (d) June 1993. 25...

McMillan, Stephen Randall

1995-01-01T23:59:59.000Z

220

Modeling Techniques Used to Analyze Safety of Payloads for Generic Missile Type Weapons Systems During an Indirect Lightning Strike  

SciTech Connect (OSTI)

During lightning strikes buildings and other structures can act as imperfect Faraday Cages, enabling electromagnetic fields to be developed inside the facilities. Some equipment stored inside these facilities may unfortunately act as antenna systems. It is important to have techniques developed to analyze how much voltage, current, or energy dissipation may be developed over valuable components. In this discussion we will demonstrate the modeling techniques used to accurately analyze a generic missile type weapons system as it goes through different stages of assembly. As work is performed on weapons systems detonator cables can become exposed. These cables will form different monopole and loop type antenna systems that must be analyzed to determine the voltages developed over the detonator regions. Due to the low frequencies of lightning pulses, a lumped element circuit model can be developed to help analyze the different antenna configurations. We will show an example of how numerical modeling can be used to develop the lumped element circuit models used to calculate voltage, current, or energy dissipated over the detonator region of a generic missile type weapons system.

Perkins, M P; Ong, M M; Crull, E W; Brown Jr., C G

2009-07-21T23:59:59.000Z

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


221

IMPEE PhD Opportunity Project title: LightFoot PhD A PhD Investigation Lightning Protection of HV Overhead Lines with Non-Ideal  

E-Print Network [OSTI]

to simulate this behaviour. This is to provide guidance to overhead line design engineers on lightening Overhead Lines with Non-Ideal Tower Footing Resistance Supervisor(s): Dr Jonathan Swingler j.swingler@hw.ac.uk Abstract LightFoot PhD ­ A PhD Investigation Lightning Protection of HV Overhead Lines with Non-Ideal Tower

Greenaway, Alan

222

Supramolecular DNA nanotechnology : discrete nanoparticle organization, three-dimensional DNA construction, and molecule templated DNA assembly.  

E-Print Network [OSTI]

??The field of structural DNA nanotechnology utilizes DNA's powerful base-pairing molecular recognition criteria to help solve real challenges facing researchers in material science and nanotechnology,Ö (more)

Aldaye, Faisal A., 1979-

2008-01-01T23:59:59.000Z

223

Patterning nanocrystals using DNA  

SciTech Connect (OSTI)

One of the goals of nanotechnology is to enable programmed self-assembly of patterns made of various materials with nanometer-sized control. This dissertation describes the results of experiments templating arrangements of gold and semiconductor nanocrystals using 2'-deoxyribonucleic acid (DNA). Previously, simple DNA-templated linear arrangements of two and three nanocrystals structures have been made.[1] Here, we have sought to assemble larger and more complex nanostructures. Gold-DNA conjugates with 50 to 100 bases self-assembled into planned arrangements using strands of DNA containing complementary base sequences. We used two methods to increase the complexity of the arrangements: using branched synthetic doublers within the DNA covalent backbone to create discrete nanocrystal groupings, and incorporating the nanocrystals into a previously developed DNA lattice structure [2][3] that self-assembles from tiles made of DNA double-crossover molecules to create ordered nanoparticle arrays. In the first project, the introduction of a covalently-branched synthetic doubler reagent into the backbone of DNA strands created a branched DNA ''trimer.'' This DNA trimer templated various structures that contained groupings of three and four gold nanoparticles, giving promising, but inconclusive transmission electron microscopy (TEM) results. Due to the presence of a variety of possible structures in the reaction mixtures, and due to the difficulty of isolating the desired structures, the TEM and gel electrophoresis results for larger structures having four particles, and for structures containing both 5 and 10 nm gold nanoparticles were inconclusive. Better results may come from using optical detection methods, or from improved sample preparation. In the second project, we worked toward making two-dimensional ordered arrays of nanocrystals. We replicated and improved upon previous results for making DNA lattices, increasing the size of the lattices to a length greater than 20 {micro}m, and collecting atomic force microscopy (AFM) images up to 30 {micro}m. We found the lattices' requirement of divalent magnesium cations to stabilize Holliday junctions to be incompatible with the stability of charge-stabilized gold nanoparticles used for the experiments here, and gold particles added indiscriminately to the lattice surface through non-specific binding. Redesigning the lattices to avoid magnesium may improve results.

Williams, Shara Carol

2003-09-01T23:59:59.000Z

224

Strandwise translocation of a DNA glycosylase on undamaged DNA  

SciTech Connect (OSTI)

Base excision repair of genotoxic nucleobase lesions in the genome is critically dependent upon the ability of DNA glycosylases to locate rare sites of damage embedded in a vast excess of undamaged DNA, using only thermal energy to fuel the search process. Considerable interest surrounds the question of how DNA glycosylases translocate efficiently along DNA while maintaining their vigilance for target damaged sites. Here, we report the observation of strandwise translocation of 8-oxoguanine DNA glycosylase, MutM, along undamaged DNA. In these complexes, the protein is observed to translocate by one nucleotide on one strand while remaining untranslocated on the complementary strand. We further report that alterations of single base-pairs or a single amino acid substitution (R112A) can induce strandwise translocation. Molecular dynamics simulations confirm that MutM can translocate along DNA in a strandwise fashion. These observations reveal a previously unobserved mode of movement for a DNA-binding protein along the surface of DNA.

Qi, Yan; Nam, Kwangho; Spong, Marie C.; Banerjee, Anirban; Sung, Rou-Jia; Zhang, Michael; Karplus, Martin; Verdine, Gregory L. (Harvard)

2012-05-14T23:59:59.000Z

225

Coarse-graining DNA for simulations of DNA nanotechnology  

E-Print Network [OSTI]

To simulate long time and length scale processes involving DNA it is necessary to use a coarse-grained description. Here we provide an overview of different approaches to such coarse graining, focussing on those at the nucleotide level that allow the self-assembly processes associated with DNA nanotechnology to be studied. OxDNA, our recently-developed coarse-grained DNA model, is particularly suited to this task, and has opened up this field to systematic study by simulations. We illustrate some of the range of DNA nanotechnology systems to which the model is being applied, as well as the insights it can provide into fundamental biophysical properties of DNA.

Doye, Jonathan P K; Louis, Ard A; Romano, Flavio; Sulc, Petr; Matek, Christian; Snodin, Benedict E K; Rovigatti, Lorenzo; Schreck, John S; Harrison, Ryan M; Smith, William P J

2013-01-01T23:59:59.000Z

226

Pulse trains that are characteristic of preliminary breakdown in cloud-to-ground lightning but are not followed by return stroke pulses  

E-Print Network [OSTI]

but are not followed by return stroke pulses Amitabh Nag1 and Vladimir A. Rakov1 Received 1 February 2007; revised 26-to-ground discharges but are not followed by return stroke waveforms. We assume that such trains are manifestations-to-ground lightning but are not followed by return stroke pulses, J. Geophys. Res., 113, D01102, doi:10.1029/2007JD

Florida, University of

227

Exploration of the meteorological characteristics leading to the rapid cessation of cloud-to-ground lightning in winter cyclones along the East Coast of the United States  

E-Print Network [OSTI]

Page DEDICATION. V ACKNOWLEDGEMENTS. Vl TABLE OF CONTENTS. Vl1 LIST OF FIGURES. . LIST OF TABLES. CHAPTER Xlv I HUTRODUCTION. II BACKGROUND. 1. Winter Thunderstorms. . 2. Cloud Electrification-Charging Mechanisms... for the northeast quadrant of all seven East Coast cyclones. 112 7 Same as Table 6, except for the NICG lightning sounding data. . 1 13 8 Same as Table 6, except for the southeast quadrant. . . 116 9 Same as Table 7, except for the southeast quadrant. . . . 10...

Demetriades, Nicholas William Snow

1999-01-01T23:59:59.000Z

228

DNA polymerase having modified nucleotide binding site for DNA sequencing  

DOE Patents [OSTI]

A modified gene encoding a modified DNA polymerase is disclosed. The modified polymerase incorporates dideoxynucleotides at least 20-fold better compared to the corresponding deoxynucleotides as compared with the corresponding naturally-occurring DNA polymerase. 6 figs.

Tabor, S.; Richardson, C.

1997-03-25T23:59:59.000Z

229

DNA Structural Nanotechnology Duke University  

E-Print Network [OSTI]

DNA Structural Nanotechnology John Reif Duke University Graduate Students: Harish Chandran&Caltech Tube Lattices #12;Ned Seeman New York University, USA Ned Seeman: Father of DNA Nanotechnology His Initial Ideas & Motivation for DNA Nanotechnology #12;Cube Chen & Seeman, Nature350:631 (1991) Truncated

Reif, John H.

230

Annual Report: 2010-2011 Storm Season Sampling For NON-DRY DOCK STORMWATER MONITORING FOR PUGET SOUND NAVAL SHIPYARD, BREMERTON, WA  

SciTech Connect (OSTI)

This interim report summarizes the stormwater monitoring conducted for non-dry dock outfalls in both the confined industrial area and the residential areas of Naval Base Kitsap within the Puget Sound Naval Shipyard (referred to as the Shipyard). This includes the collection, analyses, and descriptive statistics for stormwater sampling conducted from November 2010 through April 2011. Seven stormwater basins within the Shipyard were sampled during at least three storm events to characterize non-dry dock stormwater discharges at selected stormwater drains located within the facility. This serves as the Phase I component of the project and Phase II is planned for the 2011-2012 storm season. These data will assist the Navy, USEPA, Ecology and other stakeholders in understanding the nature and condition of stormwater discharges from the Shipyard and inform the permitting process for new outfall discharges. The data from Phase I was compiled with current stormwater data available from the Shipyard, Sinclair/Dyes Inlet watershed, and Puget Sound in order to support technical investigations for the Draft NPDES permit. The permit would require storm event sampling at selected stormwater drains located within the Shipyard. However, the data must be considered on multiple scales to truly understand potential impairments to beneficial uses within Sinclair and Dyes Inlets.

Brandenberger, Jill M.; Metallo, David; Johnston, Robert K.; Gebhardt, Christine; Hsu, Larry

2012-09-01T23:59:59.000Z

231

Myosin IIA participates in docking of Glut4 storage vesicles with the plasma membrane in 3T3-L1 adipocytes  

SciTech Connect (OSTI)

In adipocytes and myocytes, insulin stimulation translocates glucose transporter 4 (Glut4) storage vesicles (GSVs) from their intracellular storage sites to the plasma membrane (PM) where they dock with the PM. Then, Glut4 is inserted into the PM and initiates glucose uptake into these cells. Previous studies using chemical inhibitors demonstrated that myosin II participates in fusion of GSVs and the PM and increase in the intrinsic activity of Glut4. In this study, the effect of myosin IIA on GSV trafficking was examined by knocking down myosin IIA expression. Myosin IIA knockdown decreased both glucose uptake and exposures of myc-tagged Glut4 to the cell surface in insulin-stimulated cells, but did not affect insulin signal transduction. Interestingly, myosin IIA knockdown failed to decrease insulin-dependent trafficking of Glut4 to the PM. Moreover, in myosin IIA knockdown cells, insulin-stimulated binding of GSV SNARE protein, vesicle-associated membrane protein 2 (VAMP2) to PM SNARE protein, syntaxin 4 was inhibited. These data suggest that myosin IIA plays a role in insulin-stimulated docking of GSVs to the PM in 3T3-L1 adipocytes through SNARE complex formation.

Chung, Le Thi Kim, E-mail: ngocanh@nutr.med.tokushima-u.ac.jp [Department of Nutrition and Metabolism, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8503 (Japan); Hosaka, Toshio [Department of Public Health and Applied Nutrition, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima (Japan)] [Department of Public Health and Applied Nutrition, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima (Japan); Harada, Nagakatsu; Jambaldorj, Bayasgalan; Fukunaga, Keiko; Nishiwaki, Yuka [Department of Nutrition and Metabolism, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8503 (Japan)] [Department of Nutrition and Metabolism, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8503 (Japan); Teshigawara, Kiyoshi [Clinical Research Center for Diabetes, Tokushima University Hospital, 2-50-1 Kuramoto-cho, Tokushima 770-8503 (Japan)] [Clinical Research Center for Diabetes, Tokushima University Hospital, 2-50-1 Kuramoto-cho, Tokushima 770-8503 (Japan); Sakai, Tohru [Department of Public Health and Applied Nutrition, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima (Japan)] [Department of Public Health and Applied Nutrition, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima (Japan); Nakaya, Yutaka [Department of Nutrition and Metabolism, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8503 (Japan)] [Department of Nutrition and Metabolism, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8503 (Japan); Funaki, Makoto, E-mail: m-funaki@clin.med.tokushima-u.ac.jp [Clinical Research Center for Diabetes, Tokushima University Hospital, 2-50-1 Kuramoto-cho, Tokushima 770-8503 (Japan)] [Clinical Research Center for Diabetes, Tokushima University Hospital, 2-50-1 Kuramoto-cho, Tokushima 770-8503 (Japan)

2010-01-01T23:59:59.000Z

232

Fleet DNA (Presentation)  

SciTech Connect (OSTI)

The Fleet DNA project objectives include capturing and quantifying drive cycle and technology variation for the multitude of medium- and heavy-duty vocations; providing a common data storage warehouse for medium- and heavy-duty vehicle fleet data across DOE activities and laboratories; and integrating existing DOE tools, models, and analyses to provide data-driven decision making capabilities. Fleet DNA advantages include: for Government - providing in-use data for standard drive cycle development, R&D, tech targets, and rule making; for OEMs - real-world usage datasets provide concrete examples of customer use profiles; for fleets - vocational datasets help illustrate how to maximize return on technology investments; for Funding Agencies - ways are revealed to optimize the impact of financial incentive offers; and for researchers -a data source is provided for modeling and simulation.

Walkokwicz, K.; Duran, A.

2014-06-01T23:59:59.000Z

233

Chiral Mesophases of DNA  

E-Print Network [OSTI]

In the hexagonal columnar phase of chiral polymers a bias towards cholesteric twist competes with braiding along an average direction. When the chirality is strong, topological defects proliferate, leading to either a tilt grain boundary phase or a new ``moire state'' with twisted bond order. This moire phase can melt leading to a new phase: the chiral hexatic. I will discuss some recent experimental results from the NIH on DNA liquid crystals in the context of these theories.

Randall D. Kamien

1998-12-08T23:59:59.000Z

234

DNA waves and water  

E-Print Network [OSTI]

Some bacterial and viral DNA sequences have been found to induce low frequency electromagnetic waves in high aqueous dilutions. This phenomenon appears to be triggered by the ambient electromagnetic background of very low frequency. We discuss this phenomenon in the framework of quantum field theory. A scheme able to account for the observations is proposed. The reported phenomenon could allow to develop highly sensitive detection systems for chronic bacterial and viral infections.

L. Montagnier; J. Aissa; E. Del Giudice; C. Lavallee; A. Tedeschi; G. Vitiello

2010-12-23T23:59:59.000Z

235

Genome-Wide Identification and 3D Modeling of Proteins involved in DNA Damage Recognition and Repair (Final Report)  

SciTech Connect (OSTI)

OAK-B135 DNA Damage Recognition and Repair (DDR and R) proteins play a critical role in cellular responses to low-dose radiation and are associated with cancer. the authors have performed a systematic, genome-wide computational analysis of genomic data for human genes involved in the DDR and R process. The significant achievements of this project include: (1) Construction of the computational pipeline for searching DDR and R genes, building and validation of 3D models of proteins involved in DDR and R; (2) Functional and structural annotation of the 3D models and generation of comprehensive lists of suggested knock-out mutations; (3) Important improvement of macromolecular docking technology and its application to predict the DNA-Protein complex conformation; (4) Development of a new algorithm for improved analysis of high-density oligonucleotide arrays for gene expression profiling; (5) Construction and maintenance of the DNA Damage Recognition and Repair Database; and (6) Producing 14 research papers (10 published and 4 in preparation).

Ruben A. Abagyan, PhD

2004-04-15T23:59:59.000Z

236

A Model for Structure and Thermodynamics of ssDNA and dsDNA Near...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Structure and Thermodynamics of ssDNA and dsDNA Near a Surface:A Coarse Grained Approach. A Model for Structure and Thermodynamics of ssDNA and dsDNA Near a Surface:A Coarse...

237

Integrated Microfluidic Electrochemical DNA Sensor  

E-Print Network [OSTI]

of small scale fluid flow Laminar flow Easy to predict the flow patterns Very little diffusion This can make mixing difficult Small volumes Don't need to waste expensive reagents Easy fluid control;DNA Purification The DNA will be extracted using Invitrogen Charge Switch beads. Cellular Lysis

Fygenson, Deborah Kuchnir

238

DNA UPTAKE BY TRANSFORMABLE BACTERIA  

SciTech Connect (OSTI)

The various processes of DNA uptake by cells can be categorized as: viral DNA entry, conjugation, or transformation. Within each category, a variety of mechanisms have been found. However, considerable similarities occur among the different mechanisms of conjugation and, especially, transformation. All of these natural mechanisms of DNA transfer are quite elaborate and involve multiple protein components, as the case may be, of the virus, the donor cell, and the recipient cell. The mechanisms of viral infection and conjugation will be discussed mainly with respect to their relevance to transformation.

LACKS,S.A.

1999-09-07T23:59:59.000Z

239

Sequence independent amplification of DNA  

DOE Patents [OSTI]

The present invention is a rapid sequence-independent amplification procedure (SIA). Even minute amounts of DNA from various sources can be amplified independent of any sequence requirements of the DNA or any a priori knowledge of any sequence characteristics of the DNA to be amplified. This method allows, for example, the sequence independent amplification of microdissected chromosomal material and the reliable construction of high quality fluorescent in situ hybridization (FISH) probes from YACs or from other sources. These probes can be used to localize YACs on metaphase chromosomes but also--with high efficiency--in interphase nuclei. 25 figs.

Bohlander, S.K.

1998-03-24T23:59:59.000Z

240

Sequence independent amplification of DNA  

DOE Patents [OSTI]

The present invention is a rapid sequence-independent amplification procedure (SIA). Even minute amounts of DNA from various sources can be amplified independent of any sequence requirements of the DNA or any a priori knowledge of any sequence characteristics of the DNA to be amplified. This method allows, for example the sequence independent amplification of microdissected chromosomal material and the reliable construction of high quality fluorescent in situ hybridization (FISH) probes from YACs or from other sources. These probes can be used to localize YACs on metaphase chromosomes but also--with high efficiency--in interphase nuclei.

Bohlander, Stefan K. (Chicago, IL)

1998-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


241

Normalized cDNA libraries  

DOE Patents [OSTI]

This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3' noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library.

Soares, Marcelo B. (New York, NY); Efstratiadis, Argiris (Englewood, NJ)

1997-01-01T23:59:59.000Z

242

Normalized cDNA libraries  

DOE Patents [OSTI]

This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3{prime} noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library. 4 figs.

Soares, M.B.; Efstratiadis, A.

1997-06-10T23:59:59.000Z

243

Modeling DNA Shuffling Fengzhu Sun  

E-Print Network [OSTI]

Modeling DNA Shuffling Fengzhu Sun 1Department of Genetics Emory University School of Medicine property are selected. Irvine et al. (1991) and Sun et al. (1996) studied in vitro evolution not involving

Sun, Fengzhu - Sun, Fengzhu

244

Unnatural nucleotides for DNA sequencing  

E-Print Network [OSTI]

Fluorescent nucleotide analogs were prepared and tested to find surrogate structures that are: (i) incorporated by DNA polymerases; (ii) spectroscopically distinct for each fluorescent tag; and (iii) easily deprotected at the 3'-position under mild...

Jacutin, Swanee E

1997-01-01T23:59:59.000Z

245

Molecular Computing with DNA Self-Assembly  

E-Print Network [OSTI]

Molecular Computing with DNA Self-Assembly Urmi Majumder #12;Self-Assembly in Nature #12;Key to DNA for Molecular Computing with DNA Self-Assembly Compact: Small library of assembly primitives Complex: Capable in Tiling Assembly: vitroation tural DNA self-assembly has powerful echanisms for error correction

Reif, John H.

246

CONSULTATION RESPONSE The Forensic Use of DNA and the National DNA Database  

E-Print Network [OSTI]

CONSULTATION RESPONSE The Forensic Use of DNA and the National DNA Database Wellcome Trust response on the important topic of the forensic use of DNA and the National DNA Database (NDNAD). Given the Trust Assembly; "Forensic DNA Databasing: A European perspective" - a biomedical ethics grant to Professor Robin

Rambaut, Andrew

247

GRED Studies and Drilling of Americulture State 2, Americulture...  

Open Energy Info (EERE)

and Drilling of Americulture State 2, Americulture Tilapia Farm: Lightning Dock KGRA, Las Animas Valley, New Mexico Jump to: navigation, search OpenEI Reference LibraryAdd to...

248

Evidence for Large-Scale Laramide Tectonic Inversion and a Mid...  

Open Energy Info (EERE)

Scale Laramide Tectonic Inversion and a Mid-Tertiary Caldera Ring Fracture Zone at the Lightning Dock Geothermal System, New Mexico Jump to: navigation, search OpenEI Reference...

249

DNA polymorphism identity determination using flow cytometry  

DOE Patents [OSTI]

DNA polymorphism identity determination using flow cytometry. Primers designed to be immobilized on microspheres are allowed to anneal to the DNA strand under investigation, and are extended by either DNA polymerase using fluorescent dideoxynucleotides or ligated by DNA ligase to fluorescent reporter oligonucleotides. The fluorescence of either the dideoxynucleotide or the reporter oligonucleotide attached to the immobilized primer is measured by flow cytometry, thereby identifying the nucleotide polymorphism on the DNA strand.

Nolan, John P. (Santa Fe, NM); White, P. Scott (Los Alamos, NM); Cai, Hong (Los Alamos, NM)

2001-01-01T23:59:59.000Z

250

Cellular responses to environmental DNA damage  

SciTech Connect (OSTI)

This volume contains the proceedings of the conference entitled Cellular Responses to Environmental DNA Damage held in Banff,Alberta December 1--6, 1991. The conference addresses various aspects of DNA repair in sessions titled DNA repair; Basic Mechanisms; Lesions; Systems; Inducible Responses; Mutagenesis; Human Population Response Heterogeneity; Intragenomic DNA Repair Heterogeneity; DNA Repair Gene Cloning; Aging; Human Genetic Disease; and Carcinogenesis. Individual papers are represented as abstracts of about one page in length.

Not Available

1994-08-01T23:59:59.000Z

251

Regulation of DNA damage tolerance : studies of the translesion synthesis DNA ploymerase eta in Saccharomyces cerevisiae  

E-Print Network [OSTI]

All organisms must control the effects of DNA damage to protect the integrity of their genomes. In addition to DNA repair, this requires DNA damage tolerance pathways, which allow the continuation of essential processes ...

Woodruff, Rachel Van Etten

2008-01-01T23:59:59.000Z

252

Global Lightning Observations  

E-Print Network [OSTI]

;Climatology: Basics ¬∑ 5 years of OTD, 6 years of LIS data ¬∑ Adjusted for detection efficiency J.Atmos. Oc ¬∑ Global flash rate: 45 fl / sec ¬Ī 10% J. Geophys. Res., 2003 #12;High Resolution Full Climatology Annual (4/95-3/00) and LIS (1/98-12/03) instruments #12;#12;#12;Climatology: Diurnal cycle ( Local hour

California at Berkeley, University of

253

LIGHTNING SYMPOSIUM November 16  

E-Print Network [OSTI]

Flash Drought Dan Marsch ISTC Illinois Conservation of Resources and Energy (ICORE) Project Glenn of Illinois. A rmation, contact the mposium 2011 Mummy Steve Brown ISGS Glacial Geology and History of Lake County, Illinois Jim Angel ISWS 2011

Taylor, Steven J.

254

ARM - Measurement - Lightning stroke  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr May JunDatastreamsmmcrcalgovInstrumentsruc Documentation RUC : XDCResearch Related

255

DNA Concentration By UV Spectrophotometry Measure Absorption  

E-Print Network [OSTI]

DNA Concentration By UV Spectrophotometry Measure Absorption: 1. Dilute DNA to 0.5 to 50 ¬Ķg 2. Measure absorption at 260 nm (A260). Start by zeroing instrument with TE buffer or dH2O alone

Aris, John P.

256

Micropatterned cell arrays for detecting DNA damage  

E-Print Network [OSTI]

Numerous agents are capable of interacting with DNA and damaging it. Permanent changes in the DNA structure can be both mutagenic and cytotoxic; therefore, methods to measure the susceptibility of cells to mutations are ...

Mittal, Sukant

2008-01-01T23:59:59.000Z

257

Towards Privacy Preserving of Forensic DNA Databases  

E-Print Network [OSTI]

Protecting privacy of individuals is critical for forensic genetics. In a kinship/identity testing, related DNA profiles between user's query and the DNA database need to be extracted. However, unrelated profiles cannot be revealed to each other...

Liu, Sanmin

2012-02-14T23:59:59.000Z

258

Chromosome specific repetitive DNA sequences  

DOE Patents [OSTI]

A method is provided for determining specific nucleotide sequences useful in forming a probe which can identify specific chromosomes, preferably through in situ hybridization within the cell itself. In one embodiment, chromosome preferential nucleotide sequences are first determined from a library of recombinant DNA clones having families of repetitive sequences. Library clones are identified with a low homology with a sequence of repetitive DNA families to which the first clones respectively belong and variant sequences are then identified by selecting clones having a pattern of hybridization with genomic DNA dissimilar to the hybridization pattern shown by the respective families. In another embodiment, variant sequences are selected from a sequence of a known repetitive DNA family. The selected variant sequence is classified as chromosome specific, chromosome preferential, or chromosome nonspecific. Sequences which are classified as chromosome preferential are further sequenced and regions are identified having a low homology with other regions of the chromosome preferential sequence or with known sequences of other family me This invention is the result of a contract with the Department of Energy (Contract No. W-7405-ENG-36).

Moyzis, Robert K. (Los Alamos, NM); Meyne, Julianne (Los Alamos, NM)

1991-01-01T23:59:59.000Z

259

International Standards in Forensic DNA  

E-Print Network [OSTI]

International Standards in Forensic DNA John M. Butler, Ph.D. National Institute of Standards and Technology NIST Fellow & Special Assistant to the Director for Forensic Science Vice-Chair, National Commission on Forensic Science World Forensics Festival Seoul, Korea October 15, 2014 #12;Definition

260

Amplification of chromosomal DNA in situ  

DOE Patents [OSTI]

Amplification of chromosomal DNA in situ to increase the amount of DNA associated with a chromosome or chromosome region is described. The amplification of chromosomal DNA in situ provides for the synthesis of Fluorescence in situ Hybridization (FISH) painting probes from single dissected chromosome fragments, the production of cDNA libraries from low copy mRNAs and improved in Comparative Genomic Hybridization (CGH) procedures.

Christian, Allen T. (Tracy, CA); Coleman, Matthew A. (Livermore, CA); Tucker, James D. (Livermore, CA)

2002-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


261

DNA nanotubes and helical nanotapes via self-assembly of ssDNA-amphiphiles  

E-Print Network [OSTI]

DNA nanotubes and helical nanotapes via self- assembly of ssDNA-amphiphiles Timothy R. Pearcea and Efrosini Kokkoli*b DNA nanotubes were created using molecular self-assembly of single-stranded DNA (ss. The nanotube structures were formed by bilayers of amphiphiles, with the hydrophobic components forming

Kokkoli, Efie

262

Supplementary Information for: Integrating DNA Strand Displacement Circuitry with DNA Tile Self-assembly  

E-Print Network [OSTI]

Supplementary Information for: Integrating DNA Strand Displacement Circuitry with DNA Tile Self-assembly of Contents: · Supplementary Figures 1. Native polyacrylamide gel electrophoresis of DNA tile self-assembly 2. UV absorbance annealing and melting curves of DNA tile self-assembly 3. Characterization

Zhang, David Yu

263

Single-Molecule Study of DNA Polymerization Activity of HIV-1 Reverse Transcriptase on DNA Templates  

E-Print Network [OSTI]

Single-Molecule Study of DNA Polymerization Activity of HIV-1 Reverse Transcriptase on DNA unwinding protein, such as a helicase. In order to understand the mechanism of polymerization through secondary structures, we investigated the DNA polymerization activity of HIV-1 RT on long ssDNA templates

Xie, Xiaoliang Sunney

264

Probe and method for DNA detection  

DOE Patents [OSTI]

A hybridization probe containing two linear strands of DNA lights up upon hybridization to a target DNA using silver nanoclusters that have been templated onto one of the DNA strands. Hybridization induces proximity between the nanoclusters on one strand and an overhang on the other strand, which results in enhanced fluorescence emission from the nanoclusters.

Yeh, Hsin-Chih; Werner, James Henry; Sharma, Jaswinder Kumar; Martinez, Jennifer Suzanne

2013-07-02T23:59:59.000Z

265

Self-assembled DNA Structures for Nanoconstruction  

E-Print Network [OSTI]

Self-assembled DNA Structures for Nanoconstruction Hao Yan, Peng Yin, Sung Ha Park, Hanying Li methods based on DNA self-assembly. Here we review our recent experimental progress to utilize novel DNA nanostructures for self-assembly as well as for templates in the fabrication of functional nano

Yin, Peng

266

HELICASE DEPENDENT AMPLICATION transfer the DNA  

E-Print Network [OSTI]

STEP 1 STEP 2 STEP 3 HELICASE DEPENDENT AMPLICATION HELICASE DNA POLYMERASE transfer the DNA instrumentation. MOLECULAR BIOMARKERS MADE UP DNA or RNA STRANDS ARE A POWERFUL WEAPON IN DETECTING DISEASE. USING for sample preparation at the point to care using only hand generated power.... #12;

267

Binary electrokinetic separation of target DNA from background DNA primers.  

SciTech Connect (OSTI)

This report contains the summary of LDRD project 91312, titled ''Binary Electrokinetic Separation of Target DNA from Background DNA Primers''. This work is the first product of a collaboration with Columbia University and the Northeast BioDefense Center of Excellence. In conjunction with Ian Lipkin's lab, we are developing a technique to reduce false positive events, due to the detection of unhybridized reporter molecules, in a sensitive and multiplexed detection scheme for nucleic acids developed by the Lipkin lab. This is the most significant problem in the operation of their capability. As they are developing the tools for rapidly detecting the entire panel of hemorrhagic fevers this technology will immediately serve an important national need. The goal of this work was to attempt to separate nucleic acid from a preprocessed sample. We demonstrated the preconcentration of kilobase-pair length double-stranded DNA targets, and observed little preconcentration of 60 base-pair length single-stranded DNA probes. These objectives were accomplished in microdevice formats that are compatible with larger detection systems for sample pre-processing. Combined with Columbia's expertise, this technology would enable a unique, fast, and potentially compact method for detecting/identifying genetically-modified organisms and multiplexed rapid nucleic acid identification. Another competing approach is the DARPA funded IRIS Pharmaceutical TIGER platform which requires many hours for operation, and an 800k$ piece of equipment that fills a room. The Columbia/SNL system could provide a result in 30 minutes, at the cost of a few thousand dollars for the platform, and would be the size of a shoebox or smaller.

James, Conrad D.; Derzon, Mark Steven

2005-10-01T23:59:59.000Z

268

Microfluidic DNA sample preparation method and device  

DOE Patents [OSTI]

Manipulation of DNA molecules in solution has become an essential aspect of genetic analyses used for biomedical assays, the identification of hazardous bacterial agents, and in decoding the human genome. Currently, most of the steps involved in preparing a DNA sample for analysis are performed manually and are time, labor, and equipment intensive. These steps include extraction of the DNA from spores or cells, separation of the DNA from other particles and molecules in the solution (e.g. dust, smoke, cell/spore debris, and proteins), and separation of the DNA itself into strands of specific lengths. Dielectrophoresis (DEP), a phenomenon whereby polarizable particles move in response to a gradient in electric field, can be used to manipulate and separate DNA in an automated fashion, considerably reducing the time and expense involved in DNA analyses, as well as allowing for the miniaturization of DNA analysis instruments. These applications include direct transport of DNA, trapping of DNA to allow for its separation from other particles or molecules in the solution, and the separation of DNA into strands of varying lengths.

Krulevitch, Peter A. (Pleasanton, CA); Miles, Robin R. (Danville, CA); Wang, Xiao-Bo (San Diego, CA); Mariella, Raymond P. (Danville, CA); Gascoyne, Peter R. C. (Bellaire, TX); Balch, Joseph W. (Livermore, CA)

2002-01-01T23:59:59.000Z

269

Fleet DNA Project (Fact Sheet)  

SciTech Connect (OSTI)

The Fleet DNA Project - designed by the U.S. Department of Energy's National Renewable Energy Laboratory (NREL) in partnership with Oak Ridge National Laboratory - aims to accelerate the evolution of advanced vehicle development and support the strategic deployment of market-ready technologies that reduce costs, fuel consumption, and emissions. At the heart of the Fleet DNA Project is a clearinghouse of medium- and heavy-duty commercial fleet transportation data for optimizing the design of advanced vehicle technologies or for selecting a given technology to invest in. An easy-to-access online database will help vehicle manufacturers and fleets understand the broad operational range for many of today's commercial vehicle vocations.

Not Available

2012-10-01T23:59:59.000Z

270

Electromagnetic Signals from Bacterial DNA  

E-Print Network [OSTI]

Chemical reactions can be induced at a distance due to the propagation of electromagnetic signals during intermediate chemical stages. Although is is well known at optical frequencies, e.g. photosynthetic reactions, electromagnetic signals hold true for muck lower frequencies. In E. coli bacteria such electromagnetic signals can be generated by electric transitions between energy levels describing electrons moving around DNA loops. The electromagnetic signals between different bacteria within a community is a "wireless" version of intercellular communication found in bacterial communities connected by "nanowires". The wireless broadcasts can in principle be of both the AM and FM variety due to the magnetic flux periodicity in electron energy spectra in bacterial DNA orbital motions.

A. Widom; J. Swain; Y. N. Srivastava; S. Sivasubramanian

2012-02-09T23:59:59.000Z

271

Channel plate for DNA sequencing  

DOE Patents [OSTI]

This invention is a channel plate that facilitates data compaction in DNA sequencing. The channel plate has a length, a width and a thickness, and further has a plurality of channels that are parallel. Each channel has a depth partially through the thickness of the channel plate. Additionally an interface edge permits electrical communication across an interface through a buffer to a deposition membrane surface. 15 figs.

Douthart, R.J.; Crowell, S.L.

1998-01-13T23:59:59.000Z

272

The dynamic interplay between DNA damage and metabolism : the metabolic fate and transport of DNA lesions and novel DNA damage derived from intermediary metabolism  

E-Print Network [OSTI]

The work presented in this thesis explores two novel and complementary facets of endogenous DNA damage: the development of biomarkers of inflammation based on metabolites of DNA damage products and the formation of DNA ...

Jumpathong, Watthanachai

2014-01-01T23:59:59.000Z

273

DNA  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmospheric Optical Depth7-1D: Vegetation Proposed Newcatalyst phases onOrganization FY Middle SchoolARM-TR-01468This4t c 5DMSEdrives

274

Enhancing the DNA Patent Database  

SciTech Connect (OSTI)

Final Report on Award No. DE-FG0201ER63171 Principal Investigator: LeRoy B. Walters February 18, 2008 This project successfully completed its goal of surveying and reporting on the DNA patenting and licensing policies at 30 major U.S. academic institutions. The report of survey results was published in the January 2006 issue of Nature Biotechnology under the title ďThe Licensing of DNA Patents by US Academic Institutions: An Empirical Survey.Ē Lori Pressman was the lead author on this feature article. A PDF reprint of the article will be submitted to our Program Officer under separate cover. The project team has continued to update the DNA Patent Database on a weekly basis since the conclusion of the project. The database can be accessed at dnapatents.georgetown.edu. This database provides a valuable research tool for academic researchers, policymakers, and citizens. A report entitled Reaping the Benefits of Genomic and Proteomic Research: Intellectual Property Rights, Innovation, and Public Health was published in 2006 by the Committee on Intellectual Property Rights in Genomic and Protein Research and Innovation, Board on Science, Technology, and Economic Policy at the National Academies. The report was edited by Stephen A. Merrill and Anne-Marie Mazza. This report employed and then adapted the methodology developed by our research project and quoted our findings at several points. (The full report can be viewed online at the following URL: http://www.nap.edu/openbook.php?record_id=11487&page=R1). My colleagues and I are grateful for the research support of the ELSI program at the U.S. Department of Energy.

Walters, LeRoy B.

2008-02-18T23:59:59.000Z

275

DNA-guided nanoparticle assemblies  

DOE Patents [OSTI]

In some embodiments, DNA-capped nanoparticles are used to define a degree of crystalline order in assemblies thereof. In some embodiments, thermodynamically reversible and stable body-centered cubic (bcc) structures, with particles occupying <.about.10% of the unit cell, are formed. Designs and pathways amenable to the crystallization of particle assemblies are identified. In some embodiments, a plasmonic crystal is provided. In some aspects, a method for controlling the properties of particle assemblages is provided. In some embodiments a catalyst is formed from nanoparticles linked by nucleic acid sequences and forming an open crystal structure with catalytically active agents attached to the crystal on its surface or in interstices.

Gang, Oleg; Nykypanchuk, Dmytro; Maye, Mathew; van der Lelie, Daniel

2013-07-16T23:59:59.000Z

276

Human Genome Research: Decoding DNA  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmospheric Optical Depth7-1D: Vegetation ProposedUsingFun withconfinement plasmas inPortalAllBPA addressesHowHughHumanDecoding DNA

277

Assembling semiconductor nanocomposites using DNA replication technologies.  

SciTech Connect (OSTI)

Deoxyribonucleic acid (DNA) molecules represent Nature's genetic database, encoding the information necessary for all cellular processes. From a materials engineering perspective, DNA represents a nanoscale scaffold with highly refined structure, stability across a wide range of environmental conditions, and the ability to interact with a range of biomolecules. The ability to mass-manufacture functionalized DNA strands with Angstrom-level resolution through DNA replication technology, however, has not been explored. The long-term goal of the work presented in this report is focused on exploiting DNA and in vitro DNA replication processes to mass-manufacture nanocomposite materials. The specific objectives of this project were to: (1) develop methods for replicating DNA strands that incorporate nucleotides with ''chemical handles'', and (2) demonstrate attachment of nanocrystal quantum dots (nQDs) to functionalized DNA strands. Polymerase chain reaction (PCR) and primer extension methodologies were used to successfully synthesize amine-, thiol-, and biotin-functionalized DNA molecules. Significant variability in the efficiency of modified nucleotide incorporation was observed, and attributed to the intrinsic properties of the modified nucleotides. Noncovalent attachment of streptavidin-coated nQDs to biotin-modified DNA synthesized using the primer extension method was observed by epifluorescence microscopy. Data regarding covalent attachment of nQDs to amine- and thiol-functionalized DNA was generally inconclusive; alternative characterization tools are necessary to fully evaluate these attachment methods. Full realization of this technology may facilitate new approaches to manufacturing materials at the nanoscale. In addition, composite nQD-DNA materials may serve as novel recognition elements in sensor devices, or be used as diagnostic tools for forensic analyses. This report summarizes the results obtained over the course of this 1-year project.

Heimer, Brandon W.; Crown, Kevin K.; Bachand, George David

2005-11-01T23:59:59.000Z

278

Optical Recognition of Converted DNA Nucleotides for Single-Molecule DNA  

E-Print Network [OSTI]

Optical Recognition of Converted DNA Nucleotides for Single-Molecule DNA Sequencing Using Nanopore among individual nucleotides (nt). The system must be capable of differentiating among the four bases

279

Chemical biology of mutagenesis and DNA repair: cellular responses to DNA alkylation  

E-Print Network [OSTI]

The reaction of DNA-damaging agents with the genome results in a plethora of lesions, commonly referred to as adducts. Adducts may cause DNA to mutate, they may represent the chemical precursors of lethal events and they ...

Shrivastav, Nidhi

280

Nucleotide capacitance calculation for DNA sequencing  

SciTech Connect (OSTI)

Using a first-principles linear response theory, the capacitance of the DNA nucleotides, adenine, cytosine, guanine and thymine, are calculated. The difference in the capacitance between the nucleotides is studied with respect to conformational distortion. The result suggests that although an alternate current capacitance measurement of a single-stranded DNA chain threaded through a nano-gap electrodes may not sufficient to be used as a stand alone method for rapid DNA sequencing, the capacitance of the nucleotides should be taken into consideration in any GHz-frequency electric measurements and may also serve as an additional criterion for identifying the DNA sequence.

Lu, Jun-Qiang [ORNL; Zhang, Xiaoguang [ORNL

2008-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


281

A DNA Based Implementation of an Evolutionary Search for Good Encodings for DNA Computation  

E-Print Network [OSTI]

lelism, capacity, and power. This potential, however, is limited by the constraints imposed by the DNA chemistry 1]. Adleman 2] introduced a way to solve combina- torial optimization problems with DNA- mental reaction in DNA based computation is hydro- gen bonding between Watson-Crick complement base pairs

Deaton, Russell J.

282

Dynamics and control of DNA sequence amplification  

SciTech Connect (OSTI)

DNA amplification is the process of replication of a specified DNA sequence in vitro through time-dependent manipulation of its external environment. A theoretical framework for determination of the optimal dynamic operating conditions of DNA amplification reactions, for any specified amplification objective, is presented based on first-principles biophysical modeling and control theory. Amplification of DNA is formulated as a problem in control theory with optimal solutions that can differ considerably from strategies typically used in practice. Using the Polymerase Chain Reaction as an example, sequence-dependent biophysical models for DNA amplification are cast as control systems, wherein the dynamics of the reaction are controlled by a manipulated input variable. Using these control systems, we demonstrate that there exists an optimal temperature cycling strategy for geometric amplification of any DNA sequence and formulate optimal control problems that can be used to derive the optimal temperature profile. Strategies for the optimal synthesis of the DNA amplification control trajectory are proposed. Analogous methods can be used to formulate control problems for more advanced amplification objectives corresponding to the design of new types of DNA amplification reactions.

Marimuthu, Karthikeyan [Department of Chemical Engineering and Center for Advanced Process Decision-Making, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213 (United States); Chakrabarti, Raj, E-mail: raj@pmc-group.com, E-mail: rajc@andrew.cmu.edu [Department of Chemical Engineering and Center for Advanced Process Decision-Making, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213 (United States); Division of Fundamental Research, PMC Advanced Technology, Mount Laurel, New Jersey 08054 (United States)

2014-10-28T23:59:59.000Z

283

DNA Motif Representation with Nucleotide Dependency  

E-Print Network [OSTI]

DNA Motif Representation with Nucleotide Dependency Francis Chin1 and Henry Leung1 1 Department, these representations cannot model biological binding sites well because they fail to capture nucleotide interdependence. It has been pointed out by many researchers that the nucleotides of the DNA binding site cannot

Chin, Francis Y.L.

284

Carbon Nanotube DNA Sensor and Sensing Mechanism  

E-Print Network [OSTI]

Carbon Nanotube DNA Sensor and Sensing Mechanism Xiaowu Tang,*,, Sarunya Bansaruntip, Nozomi; Revised Manuscript Received June 7, 2006 ABSTRACT We report the fabrication of single-walled carbon nanotube (SWNT) DNA sensors and the sensing mechanism. The simple and generic protocol for label

Le Roy, Robert J.

285

Recombinant DNA encoding a desulfurization biocatalyst  

DOE Patents [OSTI]

This invention relates to a recombinant DNA molecule containing a gene or genes which encode a biocatalyst capable of desulfurizing a fossil fuel which contains organic sulfur molecules. For example, the present invention encompasses a recombinant DNA molecule containing a gene or genes of a strain of Rhodococcus rhodochrous. 13 figs.

Rambosek, J.; Piddington, C.S.; Kovacevich, B.R.; Young, K.D.; Denome, S.A.

1994-10-18T23:59:59.000Z

286

Electric DNA chips for determination of pathogenic microorganisms  

E-Print Network [OSTI]

Electric DNA chips for determination of pathogenic microorganisms Yanling Liu Doctoral thesis Liu (2008): Electric DNA chips for determination of pathogenic microorganisms. School of Biotechnology, Royal Institute of Technology (KTH), Stockholm, Sweden Abstract Silicon-based electric DNA chip arrays

Enfors, Sven-Olof

287

Mitochondrial DNA phylogenetic analysis of Myrmecolacidae (Insecta: Strepsiptera) va  

E-Print Network [OSTI]

Guinea are determined. Methods were developed for extraction, amplification, and sequencing of mitochondrial DNA (mtDNA) from Strepsiptera. High-resolution mtDNA sequences obtained by these methods were used in the phylogenetic analysis of Myrmecolacidae...

Halbert, Natalie Rose

2013-02-22T23:59:59.000Z

288

Single molecule analysis of DNA electrophoresis in microdevices  

E-Print Network [OSTI]

Given that current electrophoresis technology is inadequate for mapping large O[100 kilobasepair] DNA, several promising lab-on-chip designs for DNA mapping have been recently proposed that require either 1) a DNA molecule ...

Randall, Greg C

2006-01-01T23:59:59.000Z

289

Flow cytometric detection method for DNA samples  

DOE Patents [OSTI]

Disclosed herein are two methods for rapid multiplex analysis to determine the presence and identity of target DNA sequences within a DNA sample. Both methods use reporting DNA sequences, e.g., modified conventional Taqman.RTM. probes, to combine multiplex PCR amplification with microsphere-based hybridization using flow cytometry means of detection. Real-time PCR detection can also be incorporated. The first method uses a cyanine dye, such as, Cy3.TM., as the reporter linked to the 5' end of a reporting DNA sequence. The second method positions a reporter dye, e.g., FAM.TM. on the 3' end of the reporting DNA sequence and a quencher dye, e.g., TAMRA.TM., on the 5' end.

Nasarabadi,Shanavaz (Livermore, CA); Langlois, Richard G. (Livermore, CA); Venkateswaran, Kodumudi S. (Round Rock, TX)

2011-07-05T23:59:59.000Z

290

Atomic force microscopy of biochemically tagged DNA  

SciTech Connect (OSTI)

Small fragments of DNA of known length were made with the polymerase chain reaction. These fragments had biotin molecules covalently attached at their ends. They were subsequently labeled with a chimeric protein fusion between streptavidin and two immunoglobulin G-binding domains of staphyloccocal protein A. This tetrameric species was expected to bind up to four DNA molecules via their attached biotin moieties. The DNA-protein complex was deposited on mica and imaged with an atomic force microscope. The images revealed the protein chimera at the expected location at the ends of the strands of DNA as well as the expected dimers, trimers, and tetramers of DNA bound to a single protein. 25 refs., 5 figs.

Ogletree, D.F.; Kolbe, W.; Spengler, S.; Salmeron, M. (Lawrence Berkeley Laboratory, CA (United States)); Hansma, H.G.; Bezanilla, M. (Univ. of California, Santa Barbara (United States)); Sano, T.; Smith, C.S.; Cantor, C.R. (Boston Univ., MA (United States))

1993-05-01T23:59:59.000Z

291

Storing data encoded DNA in living organisms  

DOE Patents [OSTI]

Current technologies allow the generation of artificial DNA molecules and/or the ability to alter the DNA sequences of existing DNA molecules. With a careful coding scheme and arrangement, it is possible to encode important information as an artificial DNA strand and store it in a living host safely and permanently. This inventive technology can be used to identify origins and protect R&D investments. It can also be used in environmental research to track generations of organisms and observe the ecological impact of pollutants. Today, there are microorganisms that can survive under extreme conditions. As well, it is advantageous to consider multicellular organisms as hosts for stored information. These living organisms can provide as memory housing and protection for stored data or information. The present invention provides well for data storage in a living organism wherein at least one DNA sequence is encoded to represent data and incorporated into a living organism.

Wong; Pak C. (Richland, WA), Wong; Kwong K. (Sugar Land, TX), Foote; Harlan P. (Richland, WA)

2006-06-06T23:59:59.000Z

292

Flow cytometric detection method for DNA samples  

DOE Patents [OSTI]

Disclosed herein are two methods for rapid multiplex analysis to determine the presence and identity of target DNA sequences within a DNA sample. Both methods use reporting DNA sequences, e.g., modified conventional Taqman.RTM. probes, to combine multiplex PCR amplification with microsphere-based hybridization using flow cytometry means of detection. Real-time PCR detection can also be incorporated. The first method uses a cyanine dye, such as, Cy3.TM., as the reporter linked to the 5' end of a reporting DNA sequence. The second method positions a reporter dye, e.g., FAM, on the 3' end of the reporting DNA sequence and a quencher dye, e.g., TAMRA, on the 5' end.

Nasarabadi, Shanavaz (Livermore, CA); Langlois, Richard G. (Livermore, CA); Venkateswaran, Kodumudi S. (Livermore, CA)

2006-08-01T23:59:59.000Z

293

Crystal Structure of the Chromodomain Helicase DNA-binding Protein 1 (Chd1) DNA-binding Domain in Complex with DNA  

SciTech Connect (OSTI)

Chromatin remodelers are ATP-dependent machines that dynamically alter the chromatin packaging of eukaryotic genomes by assembling, sliding, and displacing nucleosomes. The Chd1 chromatin remodeler possesses a C-terminal DNA-binding domain that is required for efficient nucleosome sliding and believed to be essential for sensing the length of DNA flanking the nucleosome core. The structure of the Chd1 DNA-binding domain was recently shown to consist of a SANT and SLIDE domain, analogous to the DNA-binding domain of the ISWI family, yet the details of how Chd1 recognized DNA were not known. Here we present the crystal structure of the Saccharomyces cerevisiae Chd1 DNA-binding domain in complex with a DNA duplex. The bound DNA duplex is straight, consistent with the preference exhibited by the Chd1 DNA-binding domain for extranucleosomal DNA. Comparison of this structure with the recently solved ISW1a DNA-binding domain bound to DNA reveals that DNA lays across each protein at a distinct angle, yet contacts similar surfaces on the SANT and SLIDE domains. In contrast to the minor groove binding seen for Isw1 and predicted for Chd1, the SLIDE domain of the Chd1 DNA-binding domain contacts the DNA major groove. The majority of direct contacts with the phosphate backbone occur only on one DNA strand, suggesting that Chd1 may not strongly discriminate between major and minor grooves.

Sharma A.; Heroux A.; Jenkins K. R.; Bowman G. D.

2011-12-09T23:59:59.000Z

294

ap dna endonuclease: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

specificity Raines, Ronald T. 2 A DNA and restriction enzyme implementation of Turing Ma (Turing machines; Universal Turing machines; recombinant DNA; nonpalindromic...

295

adenovirus dna sequences: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

description of the given DNA string in terms of a smaller set of distinct domain labels. This yields a minimal domain description of a given DNA sequence, significantly...

296

Minor Groove Deformability of DNA: A Molecular Dynamics Free...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Minor Groove Deformability of DNA: A Molecular Dynamics Free EnergySimulation Study. Minor Groove Deformability of DNA: A Molecular Dynamics Free EnergySimulation Study. Abstract:...

297

Electrochemical Branched-DNA Assay for Polymerase Chain Reaction...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Branched-DNA Assay for Polymerase Chain Reaction-Free Detection and Quantification of Oncogenes in Messenger RNA Electrochemical Branched-DNA Assay for Polymerase Chain...

298

adapting dna microarray: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Gene Expression Analysis & R Tutorial 12;DNA Microarrays An R Tutorial Functional Genomics Qiu, Weigang 3 Research tools known as DNA microarrays are Biology and Medicine...

299

archived dna microarrays: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Gene Expression Analysis & R Tutorial 12;DNA Microarrays An R Tutorial Functional Genomics Qiu, Weigang 3 Research tools known as DNA microarrays are Biology and Medicine...

300

Protein Bridges DNA Base and Nucleotide Excision Repair Pathways  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

a nucleotide from the DNA double helix to its active site to access damaged nucleotides. But unlike AGT and most other known DNA nucleotide-flipping proteins, this...

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


301

Constraint of DNA on Functionalized Graphene Improves Its Biostability...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Constraint of DNA on Functionalized Graphene Improves Its Biostability and Specificity. Constraint of DNA on Functionalized Graphene Improves Its Biostability and Specificity....

302

Method of quantitating dsDNA  

DOE Patents [OSTI]

A method for quantitating dsDNA in an aqueous sample solution containing an unknown amount of dsDNA. A first aqueous test solution containing a known amount of a fluorescent dye-dsDNA complex and at least one fluorescence-attenutating contaminant is prepared. The fluorescence intensity of the test solution is measured. The first test solution is diluted by a known amount to provide a second test solution having a known concentration of dsDNA. The fluorescence intensity of the second test solution is measured. Additional diluted test solutions are similarly prepared until a sufficiently dilute test solution having a known amount of dsDNA is prepared that has a fluorescence intensity that is not attenuated upon further dilution. The value of the maximum absorbance of this solution between 200-900 nanometers (nm), referred to herein as the threshold absorbance, is measured. A sample solution having an unknown amount of dsDNA and an absorbance identical to that of the sufficiently dilute test solution at the same chosen wavelength is prepared. Dye is then added to the sample solution to form the fluorescent dye-dsDNA-complex, after which the fluorescence intensity of the sample solution is measured and the quantity of dsDNA in the sample solution is determined. Once the threshold absorbance of a sample solution obtained from a particular environment has been determined, any similarly prepared sample solution taken from a similar environment and having the same value for the threshold absorbance can be quantified for dsDNA by adding a large excess of dye to the sample solution and measuring its fluorescence intensity.

Stark, Peter C. (Los Alamos, NM); Kuske, Cheryl R. (Los Alamos, NM); Mullen, Kenneth I. (Los Alamos, NM)

2002-01-01T23:59:59.000Z

303

DNA fragment sizing and sorting by laser-induced fluorescence  

DOE Patents [OSTI]

A method is provided for sizing DNA fragments using high speed detection systems, such as flow cytometry to determine unique characteristics of DNA pieces from a sample. In one characterization the DNA piece is fragmented at preselected sites to produce a plurality of DNA fragments. The DNA piece or the resulting DNA fragments are treated with a dye effective to stain stoichiometrically the DNA piece or the DNA fragments. The fluorescence from the dye in the stained fragments is then examined to generate an output functionally related to the number of nucleotides in each one of the DNA fragments. In one embodiment, the intensity of the fluorescence emissions from each fragment is linearly related to the fragment length. The distribution of DNA fragment sizes forms a characterization of the DNA piece for use in forensic and research applications.

Hammond, Mark L. (Angier, NC); Jett, James H. (Los Alamos, NM); Keller, Richard A. (Los Alamos, NM); Marrone, Babetta L. (Los Alamos, NM); Martin, John C. (Los Alamos, NM)

1996-01-01T23:59:59.000Z

304

Sequential addition of short DNA oligos in DNA-polymerase-based synthesis reactions  

DOE Patents [OSTI]

A method of preselecting a multiplicity of DNA sequence segments that will comprise the DNA molecule of user-defined sequence, separating the DNA sequence segments temporally, and combining the multiplicity of DNA sequence segments with at least one polymerase enzyme wherein the multiplicity of DNA sequence segments join to produce the DNA molecule of user-defined sequence. Sequence segments may be of length n, where n is an odd integer. In one embodiment the length of desired hybridizing overlap is specified by the user and the sequences and the protocol for combining them are guided by computational (bioinformatics) predictions. In one embodiment sequence segments are combined from multiple reading frames to span the same region of a sequence, so that multiple desired hybridizations may occur with different overlap lengths.

Gardner, Shea N; Mariella, Jr., Raymond P; Christian, Allen T; Young, Jennifer A; Clague, David S

2013-06-25T23:59:59.000Z

305

Linear Thermodynamics of Rodlike DNA Filtration  

E-Print Network [OSTI]

Linear thermodynamics transportation theory is employed to study filtration of rodlike DNA molecules. Using the repeated nanoarray consisting of alternate deep and shallow regions, it is demonstrated that the complex ...

Li, Zirui

306

Intriguing DNA Editor Has a Structural Trigger  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

generating a lot of excitement because, unlike earlier genome-editing tools, its basic architecture doesn't have to be rebuilt each time a new section of DNA is targeted; it...

307

The Initiation of Bacterial DNA Replication  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

that the ATP-DnaA active site is in a closed configuration, which allows it to bind nucleotides using conserved residues from neighboring AAA+ protomers. The precise geometry of...

308

Deoxyribose oxidation chemistry and endogenous DNA adducts  

E-Print Network [OSTI]

Endogenous and exogenous oxidants react with cellular macromolecules to generate a variety of electrophiles that react with DNA produce cytotoxic and mutagenic adducts. One source of such electrophiles is deoxyribose in ...

Zhou, Xinfeng

2006-01-01T23:59:59.000Z

309

RecA acts in trans to allow replication of damaged DNA by DNA polymerase V  

E-Print Network [OSTI]

, Michael M. Cox2 , Roger Woodgate3 & Myron F. Goodman1 The DNA polymerase V (pol V) and RecA proteins

Cox, Michael M.

310

Design of Autonomous DNA Cellular Automata , Sudheer Sahu  

E-Print Network [OSTI]

Design of Autonomous DNA Cellular Automata Peng Yin ¬° , Sudheer Sahu ¬° , Andrew J. Turberfield cellular computing devices, i.e. autonomous nano-mechanical DNA computing devices embedded in DNA lattices. As a prototype of such devices, we recently reported the design of an Autonomous DNA Turing Machine, which

Yin, Peng

311

Recombinant DNA Research in Human Subjects Duke Institutional Biosafety Committee  

E-Print Network [OSTI]

/2010 Summary. All Duke research using recombinant DNA (rDNA) in human subjects must comply with the NIH Guidelines for Research involving Recombinant DNA Molecules (available at http://oba.od.nih.gov/rdna/nih Biosafety Committee (IBC) [NIH Guidelines Section III-C and III-C-1]. The NIH defines rDNA broadly

312

Mass spectrometric characterization of sequence-specific complexes of DNA and transcription factor PU.1 DNA binding domain  

SciTech Connect (OSTI)

Electrospray ionization mass spectrometry (ESI-MS) has been used to study the noncovalent interaction of the 13.5-kDa DNA binding domain of PU.1 (PU.1-DBD) with specific double-stranded DNA (dsDNA) target molecules. Mixtures of PU.1-DBD protein and wildtype target DNA sequence yielded ESI-MS spectra showing only protein-dsDNA complex ions of 1:1 stoichiometry and free dsDNA. When PU.1-DBD protein, wild type target DNA, and a mutant target DNA lacking the consensus sequence were mixed, only the 1:1 complex with the wild-type DNA was observed, consistent with gel electrophoresis mobility shift assay results, demonstrating the observation of sequence-specific protein-dsDNA complexes using ESI-MS. 22 refs., 5 figs., 1 tab.

Cheng, Xueheng; Harms, A.C.; Bruce, J.E. [Pacific Northwest National Lab., Richland, WA (United States)] [and others] [Pacific Northwest National Lab., Richland, WA (United States); and others

1996-07-15T23:59:59.000Z

313

Optical selection and collection of DNA fragments  

DOE Patents [OSTI]

Optical selection and collection of DNA fragments. The present invention includes the optical selection and collection of large (>.mu.g) quantities of clonable, chromosome-specific DNA from a sample of chromosomes. Chromosome selection is based on selective, irreversible photoinactivation of unwanted chromosomal DNA. Although more general procedures may be envisioned, the invention is demonstrated by processing chromosomes in a conventional flow cytometry apparatus, but where no droplets are generated. All chromosomes in the sample are first stained with at least one fluorescent analytic dye and bonded to a photochemically active species which can render chromosomal DNA unclonable if activated. After passing through analyzing light beam(s), unwanted chromosomes are irradiated using light which is absorbed by the photochemically active species, thereby causing photoinactivation. As desired chromosomes pass this photoinactivation point, the inactivating light source is deflected by an optical modulator; hence, desired chromosomes are not photoinactivated and remain clonable. The selection and photoinactivation processes take place on a microsecond timescale. By eliminating droplet formation, chromosome selection rates 50 times greater than those possible with conventional chromosome sorters may be obtained. Thus, usable quantities of clonable DNA from any source thereof may be collected.

Roslaniec, Mary C. (Los Alamos, NM); Martin, John C. (Los Alamos, NM); Jett, James H. (Los Alamos, NM); Cram, L. Scott (Los Alamos, NM)

1998-01-01T23:59:59.000Z

314

DNA nanotechnology: understanding and optimisation through simulation  

E-Print Network [OSTI]

DNA nanotechnology promises to provide controllable self-assembly on the nanoscale, allowing for the design of static structures, dynamic machines and computational architectures. In this article I review the state-of-the art of DNA nanotechnology, highlighting the need for a more detailed understanding of the key processes, both in terms of theoretical modelling and experimental characterisation. I then consider coarse-grained models of DNA, mesoscale descriptions that have the potential to provide great insight into the operation of DNA nanotechnology if they are well designed. In particular, I discuss a number of nanotechnological systems that have been studied with oxDNA, a recently developed coarse-grained model, highlighting the subtle interplay of kinetic, thermodynamic and mechanical factors that can determine behaviour. Finally, new results highlighting the importance of mechanical tension in the operation of a two-footed walker are presented, demonstrating that recovery from an unintended `overstepped' configuration can be accelerated by three to four orders of magnitude by application of a moderate tension to the walker's track. More generally, the walker illustrates the possibility of biasing strand-displacement processes to affect the overall rate.

Thomas E. Ouldridge

2014-11-07T23:59:59.000Z

315

Bis[(1S)-1 4-azanediyl-1-(9-deazaadenin-9-yl)-1 4-dideoxy-5-methylsulfanyl-D-ribitol] tetrakis(hydrochloride) monohydrate: structure DFT energy and ligand docking results of a potent methylthioadenosine phosphorylase inhibitor found in different  

SciTech Connect (OSTI)

The title compound, abbreviated as 5'ThiomethylImmA, is a potent inhibitor of methylthioadenosine phosphorylase [Singh et al. (2004). Biochemistry, 43, 9-18]. The synchrotron study reported here shows that the hydrochloride salt crystallizes with two independent, nearly superimposable, dications as a monohydrate with formula 2C{sub 12}H{sub 19}N{sub 5}O{sub 2}S{sup 2+}{center_dot}4Cl{sup -}{center_dot}H{sub 2}O. Hydrogen bonding utilizing the H atoms of the dication is found to favor certain molecular conformations in the salt, which are significantly different from those found as bound in the enzyme. Ligand docking studies starting from either of these dications or related neutral structures successfully place the conformationally revised structures in the enzyme active site but only under particular hydrogen-bonding and molecular flexibility criteria. Density functional theory calculations verify the energy similarity of the indendent cations and confirm the significant energy cost of the required conformation change to the enzyme bound form. The results suggest the using crystallographically determined free ligand coordinates as starting parameters for modelling may have serious limitations.

G Gainsford; G Evans; K Johnston; M Seth

2011-12-31T23:59:59.000Z

316

Role of DNA repair protein ERCC1 in skin cancer†  

E-Print Network [OSTI]

Nucleotide excision repair (NER) is one of the major repair systems for removal of DNA lesions. The NER pathway has evolved mainly to repair UV-induced DNA damage and is also active against a broad range of endogenously ...

Song, Liang

2009-01-01T23:59:59.000Z

317

DNA hybridization : fundamental studies and applications in directed assembly  

E-Print Network [OSTI]

Programmed self-assembly using non-covalent DNA-DNA interactions is a promising technique for the creation of next-generation functional devices for electronic, optical, and magnetic applications. This thesis develops the ...

Bajaj, Manish G. (Manish Gopal)

2005-01-01T23:59:59.000Z

318

Analysis of the structural changes caused by positive DNA supercoiling  

E-Print Network [OSTI]

The procession of helix-tracking enzymes along a DNA molecule results in the formation of supercoils in the DNA, with positive supercoiling (overwinding) generated ahead of the enzyme, and negative supercoiling (underwinding) ...

Barth, Marita Christine

2007-01-01T23:59:59.000Z

319

Lectin cDNA and transgenic plants derived therefrom  

DOE Patents [OSTI]

Transgenic plants containing cDNA encoding Gramineae lectin are described. The plants preferably contain cDNA coding for barley lectin and store the lectin in the leaves. The transgenic plants, particularly the leaves exhibit insecticidal and fungicidal properties.

Raikhel, Natasha V. (Okemos, MI)

2000-10-03T23:59:59.000Z

320

The biological and metabolic fates of endogenous DNA damage products  

E-Print Network [OSTI]

DNA and other biomolecules are subjected to damaging chemical reactions during normal physiological processes and in states of pathophysiology caused by endogenous and exogenous mechanisms. In DNA, this damage affects ...

Chan, Wan Simon

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


321

Mitigating security issues in the evolving DNA synthesis industry  

E-Print Network [OSTI]

DNA synthesis technologies are advancing at exponential rates, with production of ever longer, more complex, and less expensive sequences of double stranded DNA. This has fostered development of industrial scale design, ...

Turlington, Ralph Donald, III

2013-01-01T23:59:59.000Z

322

Flow cytometric measurement of total DNA and incorporated halodeoxyuridine  

DOE Patents [OSTI]

A method for the simultaneous flow cylometric measurement of total cellular DNA content and of the uptake of DNA precursors as a measure of DNA synthesis during various phases of the cell cycle in normal and malignant cells in vitro and in vivo is described. The method comprises reacting cells with labelled halodeoxyuridine (HdU), partially denaturing cellular DNA, adding to the reaction medium monoclonal antibodies (mabs) reactive with HdU, reacting the bound mabs with a second labelled antibody, incubating the mixture with a DNA stain, and measuring simultaneously the intensity of the DNA stain as a measure of the total cellular DNA and the HdU incorporated as a measure of DNA synthesis. (ACR)

Dolbeare, F.A.; Gray, J.W.

1983-10-18T23:59:59.000Z

323

Defining functional DNA elements in the human genome  

E-Print Network [OSTI]

With the completion of the human genome sequence, attention turned to identifying and annotating its functional DNA elements. As a complement to genetic and comparative genomics approaches, the Encyclopedia of DNA Elements ...

Kellis, Manolis

324

Single cell trapping and DNA damage analysis using microwell arrays  

E-Print Network [OSTI]

With a direct link to cancer, aging, and heritable diseases as well as a critical role in cancer treatment, the importance of DNA damage is well-established. The intense interest in DNA damage in applications ranging from ...

Wood, David

325

A model for sample stacking in microcapillary DNA electrophoresis  

E-Print Network [OSTI]

Sanger's method of chain termination is the method of choice in DNA sequencing, where electrophoresis is used to separate the different sized DNA. In the past decade, microfabricated capillary devices have been developed ...

Srivastava, Alok Kumar, 1967-

2002-01-01T23:59:59.000Z

326

Photoelectrochemical array platform for genomic scale DNA synthesis  

E-Print Network [OSTI]

Molecular and synthetic biologists have increasing demand for large, high-fidelity constructs of synthetic DNA. Recent developments in harvesting oligonucleotides from DNA microarrays has proven that these can be assembled ...

Emig, Christopher Joseph

2006-01-01T23:59:59.000Z

327

Bayesian models for DNA microarray data analysis  

E-Print Network [OSTI]

as to perform future predictions. The method is applied to cancer classi?cation via cDNA microarrays. In particular, the genes BRCA1 and BRCA2 are associated with a hereditary disposition to breast cancer, and the method is used to identify the set of signi...

Lee, Kyeong Eun

2005-08-29T23:59:59.000Z

328

DNA Strands Attached Inside Single Conical Nanopores: Ionic Pore Characteristics and Insight into DNA Biophysics  

E-Print Network [OSTI]

Kathawalla et al. 1989; Heng et al. 2005; Keyser et al.due to the electric ?eld (Heng et al. 2005; Randall et al.DNA analysis. Nanomedicine Heng JB, Aksimentiev A, Ho C,

Nguyen, Gael; Howorka, Stefan; Siwy, Zuzanna S.

2011-01-01T23:59:59.000Z

329

Orientational Dynamics and Dye-DNA Interactions in a Dye-Labeled DNA Aptamer  

E-Print Network [OSTI]

We report the picosecond and nanosecond timescale rotational dynamics of a dye-labeled DNA oligonucleotide or ĎĎaptameríí designed to bind specifically to immunoglobulin E. Rotational dynamics in combination with fluorescence lifetime measurements...

Wilson, George S.; Unruh, Jay R.; Gokulrangan, Giridharan; Lushington, Gerald H.; Johnson, Carey K.

2005-05-01T23:59:59.000Z

330

DNA ruler : enhancing nanopore sizing resolution by multiple measurements on the same DNA molecule  

E-Print Network [OSTI]

Nanopores are versatile sensors for label-free detection of single molecules and particles that have attracted attention for applications such as DNA sequencing and nanoparticle analysis. Detection of single molecules or ...

Sen, Yi-Heng

2012-01-01T23:59:59.000Z

331

A User's Guide to the Encyclopedia of DNA Elements (ENCODE)  

E-Print Network [OSTI]

The mission of the Encyclopedia of DNA Elements (ENCODE) Project is to enable the scientific and medical communities to

Bernstein, Bradley E.

332

Design and Characterization of Programmable DNA Nanotubes Supporting Information  

E-Print Network [OSTI]

. DNA sample preparation: Lyophilized HPLC- or PAGE- purified DNA oligonucleotides were purchased from Integrated DNA Technologies (Coralville, IA), resuspended in water, quanti- tated by UV absorbance at 260 nm immersion and 40X/0.75 NA air objectives. Blue light was filtered from a mer- cury arc lamp thro

Fygenson, Deborah Kuchnir

333

Self-assembling DNA templates for programmed artificial biomineralization  

E-Print Network [OSTI]

Self-assembling DNA templates for programmed artificial biomineralization Enrique C. Samano created via engineered DNA self-assembly represent an important new class of soft matter. These assemblies nanostructures Our current ability to create complex DNA nanostructures via designed self- assembly owes much

Finkelstein, Gleb

334

Correct and incorrect nucleotide incorporation pathways in DNA polymerase b  

E-Print Network [OSTI]

Correct and incorrect nucleotide incorporation pathways in DNA polymerase b Ravi Radhakrishnan a nucleotide incorporations in the DNA by using a novel protocol involving energy minimizations, dynamics simu- sive transient intermediates, for nucleotide incorporation at the template/primer DNA junction. A large

Schlick, Tamar

335

DNA digestion protocol & hints Overview: Although it is pretty standard to digest DNA with restriction enzymes, here  

E-Print Network [OSTI]

Liu 4/2004 DNA digestion protocol & hints Overview: Although it is pretty standard to digest DNA in molecular biology (3.1.1-3.1.2) Materials: ∑ DNA sample in water or TE buffer ∑ 10x digestion buffer.1 to 4 Ķg 10x Digestion buffer 2 Ķl 5 Ķl Enzyme ? ? Water Rest of volume Rest of volume 2. Add the enzyme

Doering, Tamara

336

Preparation of DNA-containing extract for PCR amplification  

DOE Patents [OSTI]

Environmental samples typically include impurities that interfere with PCR amplification and DNA quantitation. Samples of soil, river water, and aerosol were taken from the environment and added to an aqueous buffer (with or without detergent). Cells from the sample are lysed, releasing their DNA into the buffer. After removing insoluble cell components, the remaining soluble DNA-containing extract is treated with N-phenacylthiazolium bromide, which causes rapid precipitation of impurities. Centrifugation provides a supernatant that can be used or diluted for PCR amplification of DNA, or further purified. The method may provide a DNA-containing extract sufficiently pure for PCR amplification within 5Ė10 minutes.

Dunbar, John M.; Kuske, Cheryl R.

2006-07-11T23:59:59.000Z

337

Distinct kinetics of human DNA ligases I, IIIalpha, IIIbeta, and IV reveal direct DNA sensing ability and differential physiological functions in DNA repair  

SciTech Connect (OSTI)

The three human LIG genes encode polypeptides that catalyze phosphodiester bond formation during DNA replication, recombination and repair. While numerous studies have identified protein partners of the human DNA ligases (hLigs), there has been little characterization of the catalytic properties of these enzymes. In this study, we developed and optimized a fluorescence-based DNA ligation assay to characterize the activities of purified hLigs. Although hLigI joins DNA nicks, it has no detectable activity on linear duplex DNA substrates with short, cohesive single-strand ends. By contrast, hLigIII{beta} and the hLigIII{alpha}/XRCC1 and hLigIV/XRCC4 complexes are active on both nicked and linear duplex DNA substrates. Surprisingly, hLigIV/XRCC4, which is a key component of the major non-homologous end joining (NHEJ) pathway, is significantly less active than hLigIII on a linear duplex DNA substrate. Notably, hLigIV/XRCC4 molecules only catalyze a single ligation event in the absence or presence of ATP. The failure to catalyze subsequent ligation events reflects a defect in the enzyme-adenylation step of the next ligation reaction and suggests that, unless there is an in vivo mechanism to reactivate DNA ligase IV/XRCC4 following phosphodiester bond formation, the cellular NHEJ capacity will be determined by the number of adenylated DNA ligaseIV/XRCC4 molecules.

Chen, Xi; Ballin, Jeff D.; Della-Maria, Julie; Tsai, Miaw-Sheue; White, Elizabeth J.; Tomkinson, Alan E.; Wilson, Gerald M.

2009-05-11T23:59:59.000Z

338

Determining orientation and direction of DNA sequences  

DOE Patents [OSTI]

Determining orientation and direction of DNA sequences. A method by which fluorescence in situ hybridization can be made strand specific is described. Cell cultures are grown in a medium containing a halogenated nucleotide. The analog is partially incorporated in one DNA strand of each chromatid. This substitution takes place in opposite strands of the two sister chromatids. After staining with the fluorescent DNA-binding dye Hoechst 33258, cells are exposed to long-wavelength ultraviolet light which results in numerous strand nicks. These nicks enable the substituted strand to be denatured and solubilized by heat, treatment with high or low pH aqueous solutions, or by immersing the strands in 2.times.SSC (0.3M NaCl+0.03M sodium citrate), to name three procedures. It is unnecessary to enzymatically digest the strands using Exo III or another exonuclease in order to excise and solubilize nucleotides starting at the sites of the nicks. The denaturing/solubilizing process removes most of the substituted strand while leaving the prereplication strand largely intact. Hybridization of a single-stranded probe of a tandem repeat arranged in a head-to-tail orientation will result in hybridization only to the chromatid with the complementary strand present.

Goodwin, Edwin H. (Los Alamos, NM); Meyne, Julianne (Los Alamos, NM)

2000-01-01T23:59:59.000Z

339

Probing Minor Groove Hydrogen Bonding Interactions between RB69 DNA Polymerase and DNA  

SciTech Connect (OSTI)

Minor groove hydrogen bonding (HB) interactions between DNA polymerases (pols) and N3 of purines or O2 of pyrimidines have been proposed to be essential for DNA synthesis from results obtained using various nucleoside analogues lacking the N3 or O2 contacts that interfered with primer extension. Because there has been no direct structural evidence to support this proposal, we decided to evaluate the contribution of minor groove HB interactions with family B pols. We have used RB69 DNA pol and 3-deaza-2'-deoxyadenosine (3DA), an analogue of 2-deoxyadenosine, which has the same HB pattern opposite T but with N3 replaced with a carbon atom. We then determined pre-steady-state kinetic parameters for the insertion of dAMP opposite dT using primer/templates (P/T)-containing 3DA. We also determined three structures of ternary complexes with 3DA at various positions in the duplex DNA substrate. We found that the incorporation efficiency of dAMP opposite dT decreased 10{sup 2}-10{sup 3}-fold even when only one minor groove HB interaction was missing. Our structures show that the HB pattern and base pair geometry of 3DA/dT is exactly the same as those of dA/dT, which makes 3DA an optimal analogue for probing minor groove HB interactions between a DNA polymerase and a nucleobase. In addition, our structures provide a rationale for the observed 10{sup 2}-10{sup 3}-fold decrease in the rate of nucleotide incorporation. The minor groove HB interactions between position n-2 of the primer strand and RB69pol fix the rotomer conformations of the K706 and D621 side chains, as well as the position of metal ion A and its coordinating ligands, so that they are in the optinal orientation for DNA synthesis.

Xia, Shuangluo; Christian, Thomas D.; Wang, Jimin; Konigsberg, William H. (Yale)

2012-09-17T23:59:59.000Z

340

Osmotic pressure: resisting or promoting DNA ejection from phage  

E-Print Network [OSTI]

Recent in vitro experiments have shown that DNA ejection from bacteriophage can be partially stopped by surrounding osmotic pressure when ejected DNA is digested by DNase I on the course of ejection. We argue in this work by combination of experimental techniques (osmotic suppression without DNaseI monitored by UV absorbance, pulse-field electrophoresis, and cryo-EM visualization) and simple scaling modeling that intact genome (i.e. undigested) ejection in a crowded environment is, on the contrary, enhanced or eventually complete with the help of a pulling force resulting from DNA condensation induced by the osmotic stress itself. This demonstrates that in vivo, the osmotically stressed cell cytoplasm will promote phage DNA ejection rather than resisting it. The further addition of DNA-binding proteins under crowding conditions is shown to enhance the extent of ejection. We also found some optimal crowding conditions for which DNA content remaining in the capsid upon ejection is maximum, which correlates well...

Jeembaeva, Meerim; Larsson, Frida; Evilevitch, Alex

2008-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


341

Capstan friction model for DNA ejection from bacteriophages  

E-Print Network [OSTI]

Bacteriophages infect cells by attaching to the outer membrane and injecting their DNA into the cell.The phage DNA is then transcribed by the cell's transcription machinery.A number of physical mechanisms by which DNA can be translocated from the phage capsid into the cell have been identified. A fast ejection driven by the elastic and electrostatic potential energy of the compacted DNA within the viral capsid appears to be used by most phages, at least to initiate infection.In recent in vitro experiments, the speed of DNA translocation from a lambda phage capsid has been measured as a function of ejected length over the entire duration of the event.Here a mechanical model is proposed that is able to explain the observed dependence of exit velocity on ejected length, and that is also consistent with the accepted picture of the geometric arrangement of DNA within the viral capsid.

Sandip Ghosal

2013-01-09T23:59:59.000Z

342

How effective is graphene nanopore geometry on DNA sequencing?  

E-Print Network [OSTI]

In this paper we investigate the effects of graphene nanopore geometry on homopolymer ssDNA pulling process through nanopore using steered molecular dynamic (SMD) simulations. Different graphene nanopores are examined including axially symmetric and asymmetric monolayer graphene nanopores as well as five layer graphene polyhedral crystals (GPC). The pulling force profile, moving fashion of ssDNA, work done in irreversible DNA pulling and orientations of DNA bases near the nanopore are assessed. Simulation results demonstrate the strong effect of the pore shape as well as geometrical symmetry on free energy barrier, orientations and dynamic of DNA translocation through graphene nanopore. Our study proposes that the symmetric circular geometry of monolayer graphene nanopore with high pulling velocity can be used for DNA sequencing.

Satarifard, Vahid; Ejtehadi, Mohammad Reza

2015-01-01T23:59:59.000Z

343

Helical Disruptions in Small Loops of DNA  

E-Print Network [OSTI]

The thermodynamical stability of DNA minicircles is investigated by means of path integral techniques. Hydrogen bonds between base pairs on complementary strands can be broken by thermal fluctuations and temporary fluctuational openings along the double helix are essential to biological functions such as transcription and replication of the genetic information. Helix unwinding and bubble formation patterns are computed in circular sequences with variable radius in order to analyze the interplay between molecule size and appearance of helical disruptions. The latter are found in minicircles with $100$ base pairs and appear as a strategy to soften the stress due to the bending and torsion of the helix.

Zoli, Marco

2015-01-01T23:59:59.000Z

344

DNA Origami: A History and Current Perspective  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr May JunDatastreamsmmcrcalgovInstrumentsruc DocumentationP-Series to UserProduct: Crude OilPublicDNA Gridiron Nanostructures

345

Intriguing DNA Editor Has a Structural Trigger  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmospheric Optical Depth7-1D: Vegetation ProposedUsingFunInfrared LandResponses to EngineeredA GENERAL2RadiativeIntriguing DNA Editor

346

Intriguing DNA Editor Has a Structural Trigger  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmospheric Optical Depth7-1D: Vegetation ProposedUsingFunInfrared LandResponses to EngineeredA GENERAL2RadiativeIntriguing DNA

347

Isolation of Discrete Nanoparticle-DNA Conjugates for Plasmonic Applications  

SciTech Connect (OSTI)

Discrete DNA-gold nanoparticle conjugates with DNA lengths as short as 15 bases for both 5 nm and 20 nm gold particles have been purified by anion-exchange HPLC. Conjugates comprising short DNA (<40 bases) and large gold particles (>_ 20 nm) are difficult to purify by other means, and are potential substrates for plasmon coupling experiments. Conjugate purity is demonstrated by hybridizing complementary conjugates to form discrete structures, which are visualized by TEM.

Alivisatos, Paul; Claridge, Shelley A.; Liang, Huiyang W.; Basu, Sourav Roger; Frechet, Jean M.J.; Alivisatos, A. Paul

2008-04-11T23:59:59.000Z

348

Alternative Methods for Human Identification: Mitochondrial DNA Base Composition Profiling  

E-Print Network [OSTI]

Profiling by ESI-TOF Mass Spectrometry Kevin Kiesler Research Biologist, Applied Genetics Group Forensics · Why use Mass Spectrometry? · Abbott / Ibis Biosciences PLEX-ID Instrument · PLEX-ID mtDNA 2.0 Assay://remf.dartmouth.edu/images/mammalianLungTEM/source/8.html #12;Applied Genetics mtDNA Genotyping for Human I.D. · Mutations in mtDNA occur naturally

Perkins, Richard A.

349

Modeling DNA Interactions in Nucleosome Particles (A Computational Study of DNA Interactions in Nucleosome Particles)  

E-Print Network [OSTI]

Technology Center, University of Kansas, Lawrence, KS 66045. 2006 Annual Kansas City Area Life Sciences characterize global motions of the histone-DNA complex with respect to energy fluctuations in an assessment of energy differences resulting from the nucleotide substitutions will be presented in the context

Kansas, University of

350

Regulation of DNA Repair Fidelity by Molecular Checkpoints: "Gates" in DNA Polymerase 's Substrate Selection  

E-Print Network [OSTI]

Schlick*, Department of Chemistry and Courant Institute of Mathematical Sciences, New York UniVersity, New York, New York 10012, and Laboratory of Structural Biology, National Institute of EnVironmental Health enzymology data. Focusing on DNA polymerase (pol ), we present an emerging view of the geometric, energetic

Schlick, Tamar

351

assemble linear dna: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

compute? In his early studies of reversible computation, Bennett imagined an enzymatic Turing Machine which modified a hetero-polymer (such as DNA) to perform computation with...

352

assembly regulate dna: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

compute? In his early studies of reversible computation, Bennett imagined an enzymatic Turing Machine which modified a hetero-polymer (such as DNA) to perform computation with...

353

Fleet DNA Project - Data Dictionary for Public Download Files  

SciTech Connect (OSTI)

Reference document for the Fleet DNA results data shared on the NREL public website. The document includes variable definitions and descriptions to assist users in understanding data.

Duran, A.; Burton, E.; Kelly, K.; Walkowicz, K.

2014-09-01T23:59:59.000Z

354

Topoisomerase II Structure Suggests Novel DNA Cleavage Mechanism  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

doubly-wound polymer, processes such as chromosome compaction and replication naturally lead to DNA overwinding, underwinding, and tangling. Topoisomerases are essential to...

355

DNA-inspired materials for 'bottom-up' nanotechnology.  

E-Print Network [OSTI]

??DNA is a remarkable material that is both an inspiration for polymer nanotechnology and a versatile building block for assembling well-defined nanostructures. To create polymericÖ (more)

Ishihara, Yoshihiro.

2007-01-01T23:59:59.000Z

356

ancient human dna: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

STUDY OF DNA DOUBLE-STRAND BREAKS IN BYSTANDER PRIMARY HUMAN FIBROBLASTS L. B. Smilenov-or-nothing manner(7) . Bystander cells exhibit a variety of characteristics of...

357

ancient dna studies: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

STUDY OF DNA DOUBLE-STRAND BREAKS IN BYSTANDER PRIMARY HUMAN FIBROBLASTS L. B. Smilenov-or-nothing manner(7) . Bystander cells exhibit a variety of characteristics of...

358

Ergonomics in DNA Sequencing: Standing Down to Ergonomics  

E-Print Network [OSTI]

Ergonomics in DNA SequencingStanding Down to Ergonomics Presented at the COEH 2009We Do: Introduction ē How We Work: Ergonomics Challenges in

Naca, Christine

2009-01-01T23:59:59.000Z

359

Nanoscale topographical replication of graphene architecture by artificial DNA nanostructures  

SciTech Connect (OSTI)

Despite many studies on how geometry can be used to control the electronic properties of graphene, certain limitations to fabrication of designed graphene nanostructures exist. Here, we demonstrate controlled topographical replication of graphene by artificial deoxyribonucleic acid (DNA) nanostructures. Owing to the high degree of geometrical freedom of DNA nanostructures, we controlled the nanoscale topography of graphene. The topography of graphene replicated from DNA nanostructures showed enhanced thermal stability and revealed an interesting negative temperature coefficient of sheet resistivity when underlying DNA nanostructures were denatured at high temperatures.

Moon, Y.; Seo, S.; Park, J.; Park, T.; Ahn, J. R., E-mail: jrahn@skku.edu [Department of Physics, Sungkyunkwan University, Suwon 440-746 (Korea, Republic of); Shin, J.; Dugasani, S. R. [Sungkyunkwan Advanced Institute of Nanotechnology (SAINT), Sungkyunkwan University, Suwon 440-746 (Korea, Republic of); Woo, S. H. [College of Pharmacy, Chungnam National University, Daejeon 305-764 (Korea, Republic of); Park, S. H., E-mail: sunghapark@skku.edu [Department of Physics, Sungkyunkwan University, Suwon 440-746 (Korea, Republic of); Sungkyunkwan Advanced Institute of Nanotechnology (SAINT), Sungkyunkwan University, Suwon 440-746 (Korea, Republic of)

2014-06-09T23:59:59.000Z

360

Solvent resistant microfluidic DNA synthesizer{ Yanyi Huang,{a  

E-Print Network [OSTI]

a microfluidic DNA synthesizer out of perfluoropolyether (PFPE), an elastomer with excellent chemical characterization of the products.6 We previously reported a photocurable perfluoropolyether (PFPE) elastomer, which

Huang, Yanyi

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


361

High-Performance Integrated Genetic Analyzers for Forensic DNA Typing  

E-Print Network [OSTI]

Encoded evidence: DNA in forensic analysis. Nature Reviewspopulations. Journal of Forensic Sciences 48, 908-911 (identity testing. Journal of Forensic Sciences 51, 253-265 (

Liu, Peng

2009-01-01T23:59:59.000Z

362

ATP AND DNA AS MICROBIAL PARAMETERS IN THE ALIMENTARY TRACT  

E-Print Network [OSTI]

ATP AND DNA AS MICROBIAL PARAMETERS IN THE ALIMENTARY TRACT J. WOLSTRUP K. JENSEN A. JUST I. The aim of the present work was to investi- gate the use of ATP and DNA as parameters for microbial activity and biomass in the ali- mentary tract of cows and pigs. ATP was selected because of the promising

Paris-Sud XI, Université de

363

cDNA encoding a polypeptide including a hevein sequence  

DOE Patents [OSTI]

A cDNA clone (HEV1) encoding hevein was isolated via polymerase chain reaction (PCR) using mixed oligonucleotides corresponding to two regions of hevein as primers and a Hevea brasiliensis latex cDNA library as a template. HEV1 is 1,018 nucleotides long and includes an open reading frame of 204 amino acids.

Raikhel, N.V.; Broekaert, W.F.; Namhai Chua; Kush, A.

1993-02-16T23:59:59.000Z

364

Mechanisms of DNA Adsorption to Soil Particles Student: Maline Elumelu  

E-Print Network [OSTI]

1 Mechanisms of DNA Adsorption to Soil Particles Student: Maline Elumelu Advisor: Dr. Michael), Swiss Federal Institute of Technology (ETH), Zurich Abstract It has been found that DNA adsorbed to soil particles can be partially protected against enzymatic degradation. Soils are a very heterogeneous matrix

Fischlin, Andreas

365

Yeast Genomic Library Genomic DNA Sau3AI partial digestion  

E-Print Network [OSTI]

Yeast Genomic Library Concept: Genomic DNA ≠ Sau3AI partial digestion Vector DNA ≠ BamHI full digestion partial Ligate and transform above products Vector Information: ∑ use centromeric plasmid to avoid of the mcs Preparing Vector: 1) digest 3-4ug of library vector with BamHI for 2-4hrs in a total volume of 20

Odorizzi, Greg

366

Probing DNA shape and methylation state on a genomic scale with DNase I  

E-Print Network [OSTI]

. It approximates the size and nuclear dif- fusion properties of a typical human transcription factor (TF) (1 status of genomic DNA. Analyzing millions of DNA backbone hydrolysis events on naked genomic DNA, we show

Rohs, Remo

367

E-Print Network 3.0 - anchored dna substrates Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

show... that the binding of the -domain to DNA is not required for anchoring to DNA, as looping is still observed for Fts... in the new DNA tether (Supplementary ... Source:...

368

Extending the realm of SuNS to DNA nanoarrays and peptide features  

E-Print Network [OSTI]

Intense research on DNA arrays has been fostered by their applications in the field of biomedicine. DNA microarrays are composed of several different DNA sequences to be analyzed in parallel allowing high throughput ...

Akbulut Halatci, ÷zge

2010-01-01T23:59:59.000Z

369

E-Print Network 3.0 - active dna replication Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

DnaB(Ts), we examined the degradation... of the DnaB Helicase Leads to the Collapse and Degradation of the Replication Fork: a Comparison to UV... -induced DNA damage and...

370

Development of new tools for the production of plasmid DNA biopharmaceuticals  

E-Print Network [OSTI]

DNA vaccines and gene therapies that use plasmid DNA (pDNA) as a vector have gained attention in recent years for their good safety profile, ease of manufacturing, and potential to treat a host of diseases. With this ...

Bower, Diana M. (Diana Morgan)

2012-01-01T23:59:59.000Z

371

A DNA Damage-Induced, SOS-Independent Checkpoint Regulates Cell Division in Caulobacter crescentus  

E-Print Network [OSTI]

Cells must coordinate DNA replication with cell division, especially during episodes of DNA damage. The paradigm for cell division control following DNA damage in bacteria involves the SOS response where cleavage of the ...

Modell, Joshua W.

372

Stochastic Ratchet Mechanisms for Replacement of Proteins Bound to DNA  

E-Print Network [OSTI]

Experiments indicate that unbinding rates of proteins from DNA can depend on the concentration of proteins in nearby solution. Here we present a theory of multi-step replacement of DNA-bound proteins by solution-phase proteins. For four different kinetic scenarios we calculate the depen- dence of protein unbinding and replacement rates on solution protein concentration. We find (1) strong effects of progressive 'rezipping' of the solution-phase protein onto DNA sites liberated by 'unzipping' of the originally bound protein; (2) that a model in which solution-phase proteins bind non-specifically to DNA can describe experiments on exchanges between the non specific DNA- binding proteins Fis-Fis and Fis-HU; (3) that a binding specific model describes experiments on the exchange of CueR proteins on specific binding sites.

Simona Cocco; John F. Marko; Remi Monasson

2014-05-26T23:59:59.000Z

373

Information weights of nucleotides in DNA sequences  

E-Print Network [OSTI]

The coding sequence in DNA molecule is considered as a message to be transferred to receiver, the proteins, through a noisy information channel and each nucleotide is assigned a respective information weight. With the help of the nucleotide substitution matrix we estimated the lower bound of the amount of information carried out by nucleotides which is not subject of mutations. We used the calculated weights to reconstruct k-oligomers of genes from the Borrelia burgdorferi genome. We showed, that to this aim there is sufficient a simple rule, that the number of bits of the carried information cannot exceed some threshold value. The method introduced by us is general and applies to every genome.

M. R. Dudek; S. Cebrat; M. Kowalczuk; P. Mackiewicz; A. Nowicka; D. Mackiewicz; M. Dudkiewicz

2003-01-21T23:59:59.000Z

374

Hybridization and Selective Release of DNA Microarrays  

SciTech Connect (OSTI)

DNA microarrays contain sequence specific probes arrayed in distinct spots numbering from 10,000 to over 1,000,000, depending on the platform. This tremendous degree of multiplexing gives microarrays great potential for environmental background sampling, broad-spectrum clinical monitoring, and continuous biological threat detection. In practice, their use in these applications is not common due to limited information content, long processing times, and high cost. The work focused on characterizing the phenomena of microarray hybridization and selective release that will allow these limitations to be addressed. This will revolutionize the ways that microarrays can be used for LLNL's Global Security missions. The goals of this project were two-fold: automated faster hybridizations and selective release of hybridized features. The first study area involves hybridization kinetics and mass-transfer effects. the standard hybridization protocol uses an overnight incubation to achieve the best possible signal for any sample type, as well as for convenience in manual processing. There is potential to significantly shorten this time based on better understanding and control of the rate-limiting processes and knowledge of the progress of the hybridization. In the hybridization work, a custom microarray flow cell was used to manipulate the chemical and thermal environment of the array and autonomously image the changes over time during hybridization. The second study area is selective release. Microarrays easily generate hybridization patterns and signatures, but there is still an unmet need for methodologies enabling rapid and selective analysis of these patterns and signatures. Detailed analysis of individual spots by subsequent sequencing could potentially yield significant information for rapidly mutating and emerging (or deliberately engineered) pathogens. In the selective release work, optical energy deposition with coherent light quickly provides the thermal energy to single spots to release hybridized DNA. This work leverages LLNL expertise in optics, microfluids, and bioinformatics.

Beer, N R; Baker, B; Piggott, T; Maberry, S; Hara, C M; DeOtte, J; Benett, W; Mukerjee, E; Dzenitis, J; Wheeler, E K

2011-11-29T23:59:59.000Z

375

The Glutamate Switch of Bacteriophage T7 DNA Helicase ROLE IN COUPLING NUCLEOTIDE TRIPHOSPHATE (NTP) AND DNA BINDING TO NTP  

E-Print Network [OSTI]

The Glutamate Switch of Bacteriophage T7 DNA Helicase ROLE IN COUPLING NUCLEOTIDE TRIPHOSPHATE (NTP also creates nucleotide-binding sites located at the interfaces of the sub- units. DNA binding of a bound nucleoside 5 -triphosphate. However, in the absence of a nucleotide, Glu-343 changes orientation

Richardson, Charles C.

376

DNA Synthesis across an Abasic Lesion by Human DNA Polymerase I  

SciTech Connect (OSTI)

Abasic sites are among the most abundant DNA lesions formed in human cells, and they present a strong block to replication. DNA polymerase{sup I} (Pol{sup I}) is one of the few DNA Pols that does not follow the A-rule opposite an abasic site. We present here three structures of human Pol in complex with DNAs containing an abasic lesion and dGTP, dTTP, or dATP as the incoming nucleotide. The structures reveal a mechanism of translesion synthesis across an abasic lesion that differs from that in other Pols. Both the abasic lesion and the incoming dNTPs are intrahelical and are closely apposed across a constricted active site cleft. The dNTPs partake in distinct networks of hydrogen bonds in the 'void' opposite the lesion. These different patterns of hydrogen bonds, as well as stacking interactions, may underlie Pol's small preference for insertion of dGTP over other nucleotides opposite this common lesion.

Nair, Deepak T.; Johnson, Robert E.; Prakash, Louise; Prakash, Satya; Aggarwal, Aneel K.; (Sinai); (Texas-MED)

2009-05-22T23:59:59.000Z

377

E-Print Network 3.0 - andribosomal dna sequences Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Jiawei Hany Summary: DNA-Miner: A System Prototype for Mining DNA Sequences Jiawei Hany Hasan Jamilx Ying Luy Liangyou... biosequences is to nd sequence or repeating patterns...

378

E-Print Network 3.0 - alpha-1 foundation dna Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Medicine ; Materials Science ; Physics 76 Conductivity of a single DNA duplex bridging a carbon nanotube gap Summary: Conductivity of a single DNA duplex bridging a carbon...

379

E-Print Network 3.0 - apoptotic dna degradation Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

DNA cleavage on agarose gels and by classic apoptotic morphology (23... with Hoechst dye (Sigma) to differentiate between normal and apoptotic cells by DNA fragmentation....

380

Folding of a DNA Hairpin Loop Structure in Explicit SolventUsingRepli...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Folding of a DNA Hairpin Loop Structure in Explicit Solvent UsingReplica-Exchange Molecular Dynamics Simulations. Folding of a DNA Hairpin Loop Structure in Explicit Solvent...

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


381

E-Print Network 3.0 - alignment improves dna Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

details of initiator proteins for DNA replication have provided clues... + domains in ORC contain a conserved structural element that, in DnaA, promotes formation of a right...

382

E-Print Network 3.0 - alkyladenine dna glycosylase Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Thymine DNA... fusion TMZ Tmozolomide U373 Ligne cellulaire de glioblastomes humains Ugi Uracil-DNA-Glycosylase... recherche CNRS Rapporteur M. Jian-Sheng Sun Professeur MNHM,...

383

Sensitive method for measurement of telomeric DNA content in human tissues  

DOE Patents [OSTI]

A sensitive method for measurement of telomeric DNA content in human tissue, based upon the ratio of telomeric to centromeric DNA present in the tissue.

Bryant, Jennifer E. (Albuquerque, NM); Hutchings, Kent G. (Albuquerque, NM); Moyzis, Robert K. (Corona Del Mar, CA); Griffith, Jeffrey K. (Cedar Crest, NM)

1999-02-16T23:59:59.000Z

384

E-Print Network 3.0 - amplified polymorphic dna Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Belfast Collection: Environmental Sciences and Ecology 13 Helpful Resources on Forensic DNA Testing Butler, J.M. (2005) Forensic DNA Typing: Biology, Technology, and...

385

A novel role for the transcriptional modulator NusA in DNA repair/damage tolerance pathways in Escherichia coli  

E-Print Network [OSTI]

All organisms must contend with the consequences of DNA damage, induced by a variety of both endogenous and exogenous sources. Mechanisms of DNA repair and DNA damage tolerance are crucial for cellular survival after DNA ...

Cohen, Susan E., Ph. D. Massachusetts Institute of Technology

2009-01-01T23:59:59.000Z

386

Mobile linkers on DNA-coated colloids: valency without patches  

E-Print Network [OSTI]

Colloids coated with single-stranded DNA (ssDNA) can bind selectively to other colloids coated with complementary ssDNA. The fact that DNA-coated colloids (DNACCs) can bind to specific partners opens the prospect of making colloidal `molecules'. However, in order to design DNACC-based molecules, we must be able to control the valency of the colloids, i.e. the number of partners to which a given DNACC can bind. One obvious, but not very simple approach is to decorate the colloidal surface with patches of single-stranded DNA that selectively bind those on other colloids. Here we propose a design principle that exploits many-body effects to control the valency of otherwise isotropic colloids. Using a combination of theory and simulation, we show that we can tune the valency of colloids coated with mobile ssDNA, simply by tuning the non-specific repulsion between the particles. Our simulations show that the resulting effective interactions lead to low-valency colloids self-assembling in peculiar open structures, very different from those observed in DNACCs with immobile DNA linkers.

Stefano Angioletti-Uberti; Patrick Varilly; Bortolo M. Mognetti; Daan Frenkel

2014-08-27T23:59:59.000Z

387

Method for construction of normalized cDNA libraries  

DOE Patents [OSTI]

This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form. The method comprises: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3` noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library. 4 figs.

Soares, M.B.; Efstratiadis, A.

1996-01-09T23:59:59.000Z

388

Method for construction of normalized cDNA libraries  

DOE Patents [OSTI]

This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3' noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library.

Soares, Marcelo B. (New York, NY); Efstratiadis, Argiris (Englewood, NJ)

1996-01-01T23:59:59.000Z

389

Line narrowing spectroscopic studies of DNA-carcinogen adducts and DNA-dye complexes  

SciTech Connect (OSTI)

Laser-induced fluorescence line narrowing and non-line narrowing spectroscopic methods were applied to conformational studies of stable DNA adducts of the 7{beta}, 8{alpha}-dihydoxy-9{alpha}, l0{alpha}-epoxy-7,8,9, 10-tetrahydrobenzo[{alpha}]pyrene (anti-BPDE). Stereochemically distinct (+)-trans-, ({minus})-trans-, (+)-cis- and ({minus})-cis adducts of anti-BPDE bound to exocyclic amino group of the central guanine in an 11-mer oligonucleotide, exist in a mixture of conformations in frozen aqueous buffer matrices. The (+)-trans adduct adopts primarily an external conformation with a smaller fraction ( {approximately} 25 %) exists in a partially base-stacked conformation. Both cis adducts were found to be intercalated with significant {pi}-{pi} stacking interactions between the pyrenyl residues and the bases. Conformations of the trans-adduct of (+)-anti -BPDE in 11-mer oligonucleotides were studied as a function of flanking bases. In single stranded form the adduct at G{sub 2} or G{sub 3} (5 ft-flanking, base guanine) adopts a conformation with strong, interaction with the bases. In contrast, the adduct with a 5ft-flanking, thymine exists in a primarily helixexternal conformation. Similar differences were observed in the double stranded oligonucleotides. The nature of the 3ft-flanking base has little influence on the conformational equilibrium of the (+)-trans-anti BPDE-dG adduct. The formation and repair of BPDE-N{sup 2}-dG in DNA isolated from the skin of mice treated topically with benzo[{alpha}]pyrene (BP) was studied. Low-temperature fluorescence spectroscopy of the intact DNA identified the major adduct as (+)-trans-anti-BPDE-N-dG, and the minor adduct fraction consisted mainly of (+)-cis-anti-BPDE-N{sup 2}-dG.

Suh, Myungkoo

1995-12-06T23:59:59.000Z

390

Nuclear DNA content variation in Helianthus annus and the detection of repetitive DNA sequences  

E-Print Network [OSTI]

. This wes shaken at 140 rpm overnight at 37 C, and the bacteria were recovered by centrifugation at 10, 000Xg for 10 min at 4 C. The bacteria were resuspended in 10 ml sterile 0. 01 N and stored at 4 C. Petri dishes were poured using 10 g agarose per... liter of NZC medium, with each dish containing 30 ml of media. Dishes were used within 24 hr. Top agarose was made using 3 g agarose per liter of media. Use of agar in plates apparently interferes with restriction endonuclease activity on DNA isolated...

Michaelson, Martin Joseph

1986-01-01T23:59:59.000Z

391

automated dna sequence: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

ethanol and Na-acetate. Place the tubes in the -20 freezer overnight. (2 tubes sit in the refrigerator overnight to allow the DNA to go into solution. (8) Measure concentration...

392

anonymous dna sequences: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

ethanol and Na-acetate. Place the tubes in the -20 freezer overnight. (2 tubes sit in the refrigerator overnight to allow the DNA to go into solution. (8) Measure concentration...

393

automated dna sequencing: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

ethanol and Na-acetate. Place the tubes in the -20 freezer overnight. (2 tubes sit in the refrigerator overnight to allow the DNA to go into solution. (8) Measure concentration...

394

ancient dna sequences: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

ethanol and Na-acetate. Place the tubes in the -20 freezer overnight. (2 tubes sit in the refrigerator overnight to allow the DNA to go into solution. (8) Measure concentration...

395

Photoelectromechanical synthesis of low-cost DNA microarrays  

E-Print Network [OSTI]

Recent advances in de novo gene synthesis, library construction, and genomic selection for target sequencing using DNA from custom microarrays have demonstrated that microarrays can effectively be used as the world's ...

Chow, Brian, 1978-

2008-01-01T23:59:59.000Z

396

Method for construction of normalized cDNA libraries  

DOE Patents [OSTI]

This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3` noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to appropriate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library. This invention also provides normalized cDNA libraries generated by the above-described method and uses of the generated libraries. 19 figs.

Soares, M.B.; Efstratiadis, A.

1998-11-03T23:59:59.000Z

397

Optical studies of DNA-wrapped carbon nanotubes  

E-Print Network [OSTI]

This thesis presents a series of detailed optical studies of phonon-assisted relaxation processes in DNA-wrapped single walled carbon nanotubes. Using resonance Raman spectroscopy (RRS) and photoluminescence spectroscopy ...

Chou, Shin Grace

2006-01-01T23:59:59.000Z

398

acetobutylicum 8-oxoguanine dna: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Fabrice Sabathe; Lynn Doucette-stamm; Philippe Soucaille; Michael J. Daly; George N. Bennett; Eugene V. Koonin; Douglas R. Smith 2001-01-01 9 Role of a MutY DNA glycosylase in...

399

Spatially localized generation of nucleotide sequence-specific DNA damage  

E-Print Network [OSTI]

Spatially localized generation of nucleotide sequence-specific DNA damage Dennis H. Oh* , Brett A- neously manipulated at the nucleotide level and in three dimen- sions. This approach for targeting

Boxer, Steven G.

400

Developing a DNA vaccine to protect against brucellosis  

E-Print Network [OSTI]

Brucella are Gram-negative intracellular pathogenic bacteria, which represent a threat to human and animal health. Live vaccine strains are available to protect some animal species but no vaccines exist for human use. A DNA vaccine could potentially...

Owen, David Matthew

2013-02-22T23:59:59.000Z

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


401

Cellular responses against DNA damaged by platinum anticancer drugs  

E-Print Network [OSTI]

The anticancer activity of platinum-based drugs such as cisplatin, carboplatin, and oxaliplatin is mediated by their ability to attack DNA such that generated adducts trigger numerous cellular responses. A better understanding ...

Jung, Yongwon, 1977-

2005-01-01T23:59:59.000Z

402

Vehicle Technologies Office Merit Review 2014: Fleet DNA  

Broader source: Energy.gov [DOE]

Presentation given by National Renewable Energy Laboratory at 2014 DOE Hydrogen and Fuel Cells Program and Vehicle Technologies Office Annual Merit Review and Peer Evaluation Meeting about fleet DNA.

403

Dna electrophoresis in photopolymerized polyacrylamide gels on a microfluidic device  

E-Print Network [OSTI]

DNA gel electrophoresis is a critical analytical step in a wide spectrum of genomic analysis assays. Great efforts have been directed to the development of miniaturized microfluidic systems (ďlab-on-a-chipĒ systems) to perform low-cost, high...

Lo, Chih-Cheng

2009-05-15T23:59:59.000Z

404

Development of a microfluidic platform for integrated DNA sequencing protocols  

E-Print Network [OSTI]

This thesis describes the design and development of a microfluidic platform to reduce costs and improve the quality of in the DNA sequencing methodology currently implemented at the Broad Institute in Cambridge, Massachusetts. ...

Kumar, Mayank, S.M. Massachusetts Institute of Technology

2007-01-01T23:59:59.000Z

405

An integrated encyclopedia of DNA elements in the human genome  

E-Print Network [OSTI]

The human genome encodes the blueprint of life, but the function of the vast majority of its nearly three billion bases is unknown. The Encyclopedia of DNA Elements (ENCODE) project has systematically mapped regions of ...

Altshuler, Robert Charles

406

INTRODUCTION The homeodomain DNA-binding motif, initially discovered in  

E-Print Network [OSTI]

to the establishment and maintenance of a detailed positional reference system; homeodomain genes operating and characterization of btn cDNA and genomic clones Total RNA was prepared according to standard procedures (Sambrook

Patel, Nipam H.

407

Control for nematodes in cotton DNA story text  

E-Print Network [OSTI]

IMPACTS Control for nematodes in cotton DNA story text Studies help plants survive Battling opens in May By Donna Bowen Photo by Clemson University Site-specific controls for nematodes in cotton

Bolding, M. Chad

408

Method for rapid base sequencing in DNA and RNA  

DOE Patents [OSTI]

A method is provided for the rapid base sequencing of DNA or RNA fragments wherein a single fragment of DNA or RNA is provided with identifiable bases and suspended in a moving flow stream. An exonuclease sequentially cleaves individual bases from the end of the suspended fragment. The moving flow stream maintains the cleaved bases in an orderly train for subsequent detection and identification. In a particular embodiment, individual bases forming the DNA or RNA fragments are individually tagged with a characteristic fluorescent dye. The train of bases is then excited to fluorescence with an output spectrum characteristic of the individual bases. Accordingly, the base sequence of the original DNA or RNA fragment can be reconstructed. 2 figs.

Jett, J.H.; Keller, R.A.; Martin, J.C.; Moyzis, R.K.; Ratliff, R.L.; Shera, E.B.; Stewart, C.C.

1987-10-07T23:59:59.000Z

409

Method for rapid base sequencing in DNA and RNA  

DOE Patents [OSTI]

A method is provided for the rapid base sequencing of DNA or RNA fragments wherein a single fragment of DNA or RNA is provided with identifiable bases and suspended in a moving flow stream. An exonuclease sequentially cleaves individual bases from the end of the suspended fragment. The moving flow stream maintains the cleaved bases in an orderly train for subsequent detection and identification. In a particular embodiment, individual bases forming the DNA or RNA fragments are individually tagged with a characteristic fluorescent dye. The train of bases is then excited to fluorescence with an output spectrum characteristic of the individual bases. Accordingly, the base sequence of the original DNA or RNA fragment can be reconstructed. 2 figs.

Jett, J.H.; Keller, R.A.; Martin, J.C.; Moyzis, R.K.; Ratliff, R.L.; Shera, E.B.; Stewart, C.C.

1990-10-09T23:59:59.000Z

410

Method for rapid base sequencing in DNA and RNA  

DOE Patents [OSTI]

A method is provided for the rapid base sequencing of DNA or RNA fragments wherein a single fragment of DNA or RNA is provided with identifiable bases and suspended in a moving flow stream. An exonuclease sequentially cleaves individual bases from the end of the suspended fragment. The moving flow stream maintains the cleaved bases in an orderly train for subsequent detection and identification. In a particular embodiment, individual bases forming the DNA or RNA fragments are individually tagged with a characteristic fluorescent dye. The train of bases is then excited to fluorescence with an output spectrum characteristic of the individual bases. Accordingly, the base sequence of the original DNA or RNA fragment can be reconstructed.

Jett, James H. (Los Alamos, NM); Keller, Richard A. (Los Alamos, NM); Martin, John C. (Los Alamos, NM); Moyzis, Robert K. (Los Alamos, NM); Ratliff, Robert L. (Los Alamos, NM); Shera, E. Brooks (Los Alamos, NM); Stewart, Carleton C. (Los Alamos, NM)

1990-01-01T23:59:59.000Z

411

Linking Cell Cycle Reentry and DNA Damage in Neurodegeneration  

E-Print Network [OSTI]

Aberrant cell cycle activity and DNA damage have been observed in neurons in association with various neurodegenerative conditions. While there is strong evidence for a causative role for these events in neurotoxicity, it ...

Kim, Dohoon

412

Method for construction of normalized cDNA libraries  

DOE Patents [OSTI]

This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3' noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to appropriate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library. This invention also provides normalized cDNA libraries generated by the above-described method and uses of the generated libraries.

Soares, Marcelo B. (New York, NY); Efstratiadis, Argiris (Englewood, NJ)

1998-01-01T23:59:59.000Z

413

People V. Castro: Challenging the Forensic Use of DNA Evidence  

E-Print Network [OSTI]

Lander noted that ďat present, forensic science is virtuallydiagnose strep throat than forensic labs must meet to put aV. CASTRO: CHALLENGING THE FORENSIC USE OF DNA EVIDENCE Ü

Mnookin, Jennifer L.

2007-01-01T23:59:59.000Z

414

Lectin cDNA and transgenic plants derived therefrom  

DOE Patents [OSTI]

Transgenic plants containing cDNA encoding Gramineae lectin are described. The plants preferably contain cDNA coding for barley lectin and store the lectin in the leaves. The transgenic plants, particularly the leaves exhibit insecticidal and fungicidal properties. GOVERNMENT RIGHTS This application was funded under Department of Energy Contract DE-AC02-76ER01338. The U.S. Government has certain rights under this application and any patent issuing thereon.

Raikhel, Natasha V. (Okemos, MI)

1994-01-04T23:59:59.000Z

415

Forensic DNA data banking by state crime labortaories  

SciTech Connect (OSTI)

This article reports the results of a survey of the responsible crime laboratories in the first 19 states with legislation establishing forensic DNA data banks. The survey inquired into the labs` policies and procedures regarding the collection, storage, and analysis of samples; the retention of samples and data; search protocols; access to samples and data by third parties; and related matters. The research suggests that (1) the number of samples collected from convicted offenders for DNA data banking has far surpassed the number that have been analyzed; (2) data banks have already been used in a small but growing number of cases, to locate suspects and to identify associations between unresolved cases; (3) crime labs currently plan to retain indefinitely the samples collected for their data banks; and (4) the nature and extent of security safeguards that crime labs have implemented for their data banks vary among states. The recently enacted DNA Identification Act (1994) will provide $40 million in federal matching grants to states for DNA analysis activities, so long as states comply with specified quality-assurance standards, submit to external proficiency testing, and limit access to DNA information. Although these additional funds should help to ease some sample backlogs, it remains unclear how labs will allocate the funds, as between analyzing samples for their data banks and testing evidence samples in cases without suspects. The DNA Identification Act provides penalties for the disclosure or obtaining of DNA data held by data banks that participate in CODIS, the FBI`s evolving national network of DNA data banks, but individual crime labs must also develop stringent internal safeguards to prevent breaches of data-bank security. 9 refs., 3 tabs.

McEwen, J.E. [Eunice Kennedy Shrive Center for Mental Retardation, Waltham, MA (United States)

1995-06-01T23:59:59.000Z

416

Self-assembling DNA Nanostructures for Patterned Molecular Assembly Thomas H. LaBeana  

E-Print Network [OSTI]

1 Self-assembling DNA Nanostructures for Patterned Molecular Assembly Thomas H. LaBeana , Kurt V@cs.duke.edu; Tel: (919)660-65685 Abstract The Chapter describes the use of DNA for molecular-scale self-assembly with a discussion of DNA-nanostructures, starting with the self-assembly of various building-blocks known as DNA

Reif, John H.

417

Design of an Autonomous DNA Nanomechanical Device Capable of Universal Computation and Universal Translational Motion  

E-Print Network [OSTI]

Design of an Autonomous DNA Nanomechanical Device Capable of Universal Computation and Universal, and in DNA computing provide a solid foundation for the next step forward: designing autonomous DNA construction of autonomous unidirectional DNA walking devices that move along linear tracks, we present

Yin, Peng

418

Designs for Autonomous Unidirectional Walking DNA Devices Peng Yin Andrew J. Turberfield John H. Reif  

E-Print Network [OSTI]

Designs for Autonomous Unidirectional Walking DNA Devices Peng Yin ¬£ Andrew J. Turberfield √Ě John H. Reif √? Abstract Imagine a host of nanoscale DNA robots move autonomously over a microscale DNA chal- lenge. The missing link is a DNA walker that can autonomously move along a route programmably em

Yin, Peng

419

J. Mol. Riol. (1991) 222, 1085-1108 Complementary Recognition in Condensed DNA: Accelerated  

E-Print Network [OSTI]

J. Mol. Riol. (1991) 222, 1085-1108 Complementary Recognition in Condensed DNA: Accelerated DNA) Condensation of denatured DNA greatly accelerates the kinetics of DNA renaturation. We propose a unifying explanation for the effects of several accelerating solvents studied here including polymers, di

Church, George M.

420

A new structural framework for integrating replication protein A into DNA processing machinery  

SciTech Connect (OSTI)

By coupling the protection and organization of single-stranded DNA (ssDNA) with recruitment and alignment of DNA processing factors, replication protein A (RPA) lies at the heart of dynamic multi-protein DNA processing machinery. Nevertheless, how RPA coordinates biochemical functions of its eight domains remains unknown. We examined the structural biochemistry of RPA's DNA-binding activity, combining small-angle X-ray and neutron scattering with all-atom molecular dynamics simulations to investigate the architecture of RPA's DNA-binding core. The scattering data reveal compaction promoted by DNA binding; DNA-free RPA exists in an ensemble of states with inter-domain mobility and becomes progressively more condensed and less dynamic on binding ssDNA. Our results contrast with previous models proposing RPA initially binds ssDNA in a condensed state and becomes more extended as it fully engages the substrate. Moreover, the consensus view that RPA engages ssDNA in initial, intermediate and final stages conflicts with our data revealing that RPA undergoes two (not three) transitions as it binds ssDNA with no evidence for a discrete intermediate state. These results form a framework for understanding how RPA integrates the ssDNA substrate into DNA processing machinery, provides substrate access to its binding partners and promotes the progression and selection of DNA processing pathways.

Brosey, Chris; Yan, Chunli; Tsutakawa, Susan; Heller, William; Rambo, Robert; Tainer, John; Ivanov, Ivaylo; Chazin, Walter

2013-01-17T23:59:59.000Z

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


421

Biology Lab 3: Small Scale Plasmid DNA Purification (Minipreps) Plasmids are small, circular pieces of DNA (about 3-5 kilobases in length on average) that  

E-Print Network [OSTI]

Biology Lab 3: Small Scale Plasmid DNA Purification (Minipreps) Plasmids are small, circular pieces. Plasmid purification procedures selectively enrich plasmid DNA over genomic DNA, which is present the small plasmids remain intact. Thus, when denatured, the plasmids remain linked to their complementary

422

Plectoneme tip bubbles: Coupled denaturation and writhing in supercoiled DNA  

E-Print Network [OSTI]

Biological information is not only stored in the digital chemical sequence of double helical DNA, but is also encoded in the mechanical properties of the DNA strands, which can influence biochemical processes involving its readout. For example, loop formation in the Lac operon can regulate the expression of key genes, and DNA supercoiling is closely correlated to rhythmic circardian gene expression in cyanobacteria. Supercoiling is also important for large scale organisation of the genome in both eukaryotic and prokaryotic cells. DNA can respond to torsional stress by writhing to form looped structures called plectonemes, thus transferring energy stored as twist into energy stored in bending. Denaturation bubbles can also relax torsional stress, with the enthalpic cost of breaking bonds being compensated by their ability to absorb undertwist. Here we predict a novel regime where bubbles form at the tips of plectonemes, and study its properties using coarse-grained simulations. These tip bubbles can occur for both positive and negative supercoiling and greatly reduce plectoneme diffusion by a pinning mechanism. They can cause plectonemes to preferentially localise to AT rich regions, because bubbles more easily form there. The tip-bubble regime occurs for supercoiling densities and forces that are typically encountered for DNA in vivo, and may be exploited for biological control of genomic processes.

Christian Matek; Thomas E. Ouldridge; Jonathan P. K. Doye; Ard A. Louis

2014-04-10T23:59:59.000Z

423

Process of labeling specific chromosomes using recombinant repetitive DNA  

DOE Patents [OSTI]

Chromosome preferential nucleotide sequences are first determined from a library of recombinant DNA clones having families of repetitive sequences. Library clones are identified with a low homology with a sequence of repetitive DNA families to which the first clones respectively belong and variant sequences are then identified by selecting clones having a pattern of hybridization with genomic DNA dissimilar to the hybridization pattern shown by the respective families. In another embodiment, variant sequences are selected from a sequence of a known repetitive DNA family. The selected variant sequence is classified as chromosome specific, chromosome preferential, or chromosome nonspecific. Sequences which are classified as chromosome preferential are further sequenced and regions are identified having a low homology with other regions of the chromosome preferential sequence or with known sequences of other family members and consensus sequences of the repetitive DNA families for the chromosome preferential sequences. The selected low homology regions are then hybridized with chromosomes to determine those low homology regions hybridized with a specific chromosome under normal stringency conditions.

Moyzis, R.K.; Meyne, J.

1988-02-12T23:59:59.000Z

424

Global regulator H-NS and lipoprotein NlpI influence production of extracellular DNA in Escherichia coli  

E-Print Network [OSTI]

of DNA-containing vesicles, and DNA secretion. Here, a genome-wide screen of 3985 non-lethal mutations-type levels. Therefore our results suggest that secretion may play a role in eDNA release in E. coli since of extracellular polysaccharides, proteins, lipids, and nucleic acids [1]. The extracellular DNA (eDNA) component

Wood, Thomas K.

425

Procedure for normalization of cDNA libraries  

DOE Patents [OSTI]

This invention provides a method to normalize a cDNA library constructed in a vector capable of being converted to single-stranded circles and capable of producing complementary nucleic acid molecules to the single-stranded circles comprising: (a) converting the cDNA library in single-stranded circles; (b) generating complementary nucleic acid molecules to the single-stranded circles; (c) hybridizing the single-stranded circles converted in step (a) with complementary nucleic acid molecules of step (b) to produce partial duplexes to an appropriate Cot; (e) separating the unhybridized single-stranded circles from the hybridized single-stranded circles, thereby generating a normalized cDNA library. 1 fig.

Bonaldo, M.D.; Soares, M.B.

1997-12-30T23:59:59.000Z

426

Procedure for normalization of cDNA libraries  

DOE Patents [OSTI]

This invention provides a method to normalize a cDNA library constructed in a vector capable of being converted to single-stranded circles and capable of producing complementary nucleic acid molecules to the single-stranded circles comprising: (a) converting the cDNA library in single-stranded circles; (b) generating complementary nucleic acid molecules to the single-stranded circles; (c) hybridizing the single-stranded circles converted in step (a) with complementary nucleic acid molecules of step (b) to produce partial duplexes to an appropriate Cot; (e) separating the unhybridized single-stranded circles from the hybridized single-stranded circles, thereby generating a normalized cDNA library.

Bonaldo, Maria DeFatima (New York, NY); Soares, Marcelo Bento (New York, NY)

1997-01-01T23:59:59.000Z

427

Computational method and system for modeling, analyzing, and optimizing DNA amplification and synthesis  

DOE Patents [OSTI]

A computational method and computer-based system of modeling DNA synthesis for the design and interpretation of PCR amplification, parallel DNA synthesis, and microarray chip analysis. The method and system include modules that address the bioinformatics, kinetics, and thermodynamics of DNA amplification and synthesis. Specifically, the steps of DNA selection, as well as the kinetics and thermodynamics of DNA hybridization and extensions, are addressed, which enable the optimization of the processing and the prediction of the products as a function of DNA sequence, mixing protocol, time, temperature and concentration of species.

Vandersall, Jennifer A.; Gardner, Shea N.; Clague, David S.

2010-05-04T23:59:59.000Z

428

cDNA encoding a polypeptide including a hevein sequence  

DOE Patents [OSTI]

A cDNA clone (HEV1) encoding hevein was isolated via polymerase chain reaction (PCR) using mixed oligonucleotides corresponding to two regions of hevein as primers and a Hevea brasiliensis latex cDNA library as a template. HEV1 is 1018 nucleotides long and includes an open reading frame of 204 amino acids. The deduced amino acid sequence contains a pu GOVERNMENT RIGHTS This application was funded under Department of Energy Contract DE-AC02-76ER01338. The U.S. Government has certain rights under this application and any patent issuing thereon.

Raikhel, Natasha V. (Okemos, MI); Broekaert, Willem F. (Dilbeek, BE); Chua, Nam-Hai (Scarsdale, NY); Kush, Anil (New York, NY)

1993-02-16T23:59:59.000Z

429

Carbon Nanotubes as Electrodes for Dielectrophoresis of DNA  

E-Print Network [OSTI]

Dielectrophoresis can potentially be used as an efficient trapping tool in the fabrication of molecular devices. For nanoscale objects, however, the Brownian motion poses a challenge. We show that the use of carbon nanotube electrodes makes it possible to apply relatively low trapping voltages and still achieve high enough field gradients for trapping nanoscale objects, e.g., single molecules. We compare the efficiency and other characteristics of dielectrophoresis between carbon nanotube electrodes and lithographically fabricated metallic electrodes, in the case of trapping nanoscale DNA molecules. The results are analyzed using finite element method simulations and reveal information about the frequency dependent polarizability of DNA.

Sampo Tuukkanen; J. Jussi Toppari; Anton Kuzyk; Lasse Hirviniemi; Vesa P. Hytonen; Teemu Ihalainen; Paivi Torma

2006-07-18T23:59:59.000Z

430

Conserved and Unconventional Responses to DNA Damage in Tetrahymena  

E-Print Network [OSTI]

in the transition G1/S phase to promote the loading of DNA polymerases. Additionally CDK prevent any further activation of pre-RC. CDK also induce phosphorylation of Sld2, Sld3 and Mcm5 that promotes the initiation of DNA synthesis. Diagram modified from Sclafani... and Holzen (2007). Cdc45 Sld2-3 S-CDK Initiation G1 phase G1/S transition S phase 21 21 mechanism to restrict pre-RC formation to only once per cell cycle (Lutzmann et al., 2006). Cdc6 in budding yeast stimulates the Abf1 binding activity...

Sandoval Oporto, Pamela

2012-07-16T23:59:59.000Z

431

A comparative study of DNA polymerase alpha isolation  

E-Print Network [OSTI]

) Mechanistic Studies of DNA Re lication and Genetic Recombination, Alberts, B. , ed. Academic Press, New York, 55 53 58. Korn, D. , Fisher, P. A, , Battey, J. , and Wang, T. S. F. (1979) Cold S rin Harbor S m . Quant. Biol. 43, 613 59. Fisher, P. A...A COMPARATIVE STUDY OF DNA POLYMERASE ALPHA ISOLATION A Thesis by ZINA CERNOSEK Submitted to the Graduate College of Texas A&M University in partial fulfillment of the requirement for the degree of MASTER OF SCIENCE May 1987 Major Subject...

Cernosek, Zina

1987-01-01T23:59:59.000Z

432

DNA Origami with Complex Curvatures in Three-Dimensional Space  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr May JunDatastreamsmmcrcalgovInstrumentsruc DocumentationP-Series to UserProduct: Crude OilPublicDNA Gridiron Nanostructures BasedDNA

433

DNA-Directed Artificial Light-Harvesting Antenna  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr May JunDatastreamsmmcrcalgovInstrumentsruc DocumentationP-Series to UserProduct: Crude OilPublicDNA GridironDNA-Directed Artificial

434

Effects of Counterions on Nano-confined DNA Thin Films  

E-Print Network [OSTI]

DNA molecules spin-coated on amorphous quartz substrates are shown to form stable films. Electron density profiles (EDPs) along the film depth show that film prepared from aqueous solution of DNA exhibits layering of the molecules in three stacks parallel to the substrate whereas film prepared from counterion added solution does not have layering but have smaller thickness and enhanced surface roughness, although both films have 'liquid-like' height-height correlations. We explain these results by a model of film of a 'liquid' comprising of rod-like molecules where the counterion concentration in the liquid determines the rod length.

Nupur Biswas; Sreeja Chakraborty; Alokmay Datta; Munna Sarkar; Mrinmay K. Mukhopadhyay; Mrinal K. Bera; Hideki Seto

2014-04-03T23:59:59.000Z

435

On single-molecule DNA sequencing with atomic force microscopy using functionalized carbon nanotube probes  

E-Print Network [OSTI]

A novel DNA sequencing method is proposed based on the specific binding nature of nucleotides and measured by an atomic force microscope (AFM). A single molecule of DNA is denatured and immobilized on an atomically fiat ...

Burns, Daniel James

2004-01-01T23:59:59.000Z

436

ORTH/VIM proteins that regulate DNA methylation are functional ubiquitin E3 ligases  

E-Print Network [OSTI]

ORTH/VIM proteins that regulate DNA methylation are functional ubiquitin E3 ligases Edward Kraft1 methylation status in vivo. Keywords: RING, E3 ligase, DNA methylation, ubiquitylation, ORTH/VIM, SRA

Jacobsen, Steve

437

E-Print Network 3.0 - aromatic dna adducts Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

and EcoRI Summary: to its high reactivity toward DNA and cellular proteins. The major acrolein-DNA adduct, -hydrox- ypropano... with proteins (8) and represents a significant...

438

A study of inter-individual differences in the DNA damage response  

E-Print Network [OSTI]

Agents that damage our DNA are omnipresent in our environment and inside our cells themselves. Left unrepaired, DNA damage can lead to premature aging, neurodegeneration and cancer. Humans have thus evolved intricate and ...

Sefta, Meriem

2012-01-01T23:59:59.000Z

439

The bromodomain protein Brd4 insulates chromatin from DNA damage signalling  

E-Print Network [OSTI]

DNA damage activates a signalling network that blocks cell-cycle progression, recruits DNA repair factors and/or triggers senescence or programmed cell death. Alterations in chromatin structure are implicated in the ...

Floyd, Scott R.

440

E-Print Network 3.0 - atp-dependent dna ligases Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

ligases Search Powered by Explorit Topic List Advanced Search Sample search results for: atp-dependent dna ligases Page: << < 1 2 3 4 5 > >> 1 Profiling the selectivity of DNA...

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


441

E-Print Network 3.0 - alpha conformation dna Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

change (Kiso; see below Table 1. (A) 34 bp DNA duplexes; (B) 46 bp DNA duplexes... ESS Kd Kiso E: Enzyme in an alternative ... Source: Herschlag, Dan - Department of...

442

Site-Selective Binding of Nanoparticles to Double-Stranded DNA via Peptide Nucleic Acid "Invasion"  

SciTech Connect (OSTI)

We demonstrate a novel method for by-design placement of nano-objects along double-stranded (ds) DNA. A molecular intercalator, designed as a peptide nucleic acid (PNA)-DNA chimera, is able to invade dsDNA at the PNA-side due to the hybridization specificity between PNA and one of the duplex strands. At the same time, the single-stranded (ss) DNA tail of the chimera, allows for anchoring of nano-objects that have been functionalized with complementary ssDNA. The developed method is applied for interparticle attachment and for the fabrication of particle clusters using a dsDNA template. This method significantly broadens the molecular toolbox for constructing nanoscale systems by including the most conventional not yet utilized DNA motif, double helix DNA.

Stadler, A.L.; van der Lelie, D.; Sun, D.; Maye, M. M.; Gang, O.

2011-04-01T23:59:59.000Z

443

E-Print Network 3.0 - asynchronous dna replication Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

08.00 0 doi:10.1128JB.00408-07 Summary: of the DnaB Helicase Leads to the Collapse and Degradation of the Replication Fork: a Comparison to UV... -induced DNA damage and...

444

E-Print Network 3.0 - affect dna replication Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

08.00 0 doi:10.1128JB.00408-07 Summary: of the DnaB Helicase Leads to the Collapse and Degradation of the Replication Fork: a Comparison to UV... -induced DNA damage and...

445

E-Print Network 3.0 - accurate dna copy Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

08.00 0 doi:10.1128JB.00408-07 Summary: of the DnaB Helicase Leads to the Collapse and Degradation of the Replication Fork: a Comparison to UV... -induced DNA damage and...

446

Structures of Clamp-Loader Complexes Are Key to DNA Replication  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

DNA, so that the polymerase can move at high speed. As it sequentially copies the nucleotides that make up the DNA strand, synthesis can occur as fast as 1000 nucleotides per...

447

Regulatory pathways controlling cell division after DNA damage in Caulobacter crescentus  

E-Print Network [OSTI]

All cells must coordinate DNA replication with cell division in order to faithfully propagate whole chromosomes to daughter cells. During episodes of DNA damage, cells often delay division until the lesions have been ...

Modell, Joshua Wexler

2013-01-01T23:59:59.000Z

448

Understanding cell fate decisions in response to 0?-Methylguanine DNA lesions  

E-Print Network [OSTI]

The stability of the genome is constantly challenged by both endogenous and exogenous DNA damaging agents. DNA damage, if left unrepaired, can give rise to permanent genetic alterations that ultimately increase our risk ...

Noonan, Ericka Marie

2011-01-01T23:59:59.000Z

449

E-Print Network 3.0 - analyze dna recombination Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

depends on the host recombination genes, recB and recC. DNA sequencing showed... BCD-dependent recombination. A series of linear DNA molecules are shown in the first...

450

Sensitive method for measurement of telomeric DNA content in human tissues  

DOE Patents [OSTI]

This research discloses a sensitive method for measurement of telomeric DNA content in human tissue, based upon the ratio of telomeric to centromeric DNA present in the tissue. 5 figs.

Bryant, J.E.; Hutchings, K.G.; Moyzis, R.K.; Griffith, J.K.

1999-02-16T23:59:59.000Z

451

Coordinateendonucleolytic 5' and 3' trimming of terminally blocked blunt DNA double-strand break ends by Artemis nuclease and DNA-dependent protein kinase  

SciTech Connect (OSTI)

Previous work showed that, in the presence of DNA-PK, Artemis slowly trims 3'-phosphoglycolate-terminated blunt ends. To examine the trimming reaction in more detail, long internally labeled DNA substrates were treated with Artemis. In the absence of DNA-PK, Artemis catalyzed extensive 5' {yields} 3' exonucleolytic resection of double-stranded DNA. This resection required a 5'-phosphate but did not require ATP, and was accompanied by endonucleolytic cleavage of the resulting 3' overhang. In the presence of DNA-PK, Artemis-mediated trimming was more limited, was ATP-dependent, and did not require a 5'-phosphate. For a blunt end with either a 3'-phosphoglycolate or 3'-hydroxyl terminus, endonucleolytic trimming of 2-4 nucleotides from the 3'-terminal strand was accompanied by trimming of 6 nucleotides from the 5'-terminal strand. The results suggest that autophosphorylated DNA-PK suppresses the exonuclease activity of Artemis toward blunt-ended DNA, and promotes slow and limited endonucleolytic trimming of the 5'-terminal strand, resulting in short 3' overhangs that are trimmed endonucleolytically. Thus, Artemis and DNA-PK can convert terminally blocked DNA ends of diverse geometry and chemical structure to a form suitable for polymerase mediated patching and ligation, with minimal loss of terminal sequence. Such processing could account for the very small deletions often found at DNA double-strand break repair sites.

Povirk, Lawrence; Yannone, Steven M.; Khan, Imran S.; Zhou, Rui-Zhe; Zhou, Tong; Valerie, Kristoffer; F., Lawrence

2008-02-18T23:59:59.000Z

452

Abstract DNA-type systems Diederik Aerts1  

E-Print Network [OSTI]

example is the observation that DNA is essentially a universal Turing machine [1, 2]. The hardware. This seems to be a continuous process that resembles the motion of finite control in a real-life Turing machine. The discrete time-steps of an abstract Turing machine can be obtained if one looks at snapshots

Aerts, Diederik

453

Affinity Purification of Plasmid DNA by Temperature-Triggered Precipitation  

E-Print Network [OSTI]

E. coli cell lysates to a better purity than that prepared by a standard alkaline purification candidate for large-scale purification of plasmid DNA for sensitive applications such as nonviral gene and Doly, 1979) and CsCl-dye buoyant density-gradient ultra- centrifugation (Sambrook and Russell, 2001

Chen, Wilfred

454

Dielectrophoretic detection and quantification of hybridized DNA molecules  

E-Print Network [OSTI]

normalized cof calibration chart allows simplified quantifi- cation of the target ssDNA concentrations time consuming, typically on the order of hours to days, the standard micro-array technique­6]. The dielectrophoretic force on a particle of radius a, suspended in a medium permittivity em in an AC field of magnitude

Chang, Hsueh-Chia

455

Estimating Dataset Size Requirements for Classifying DNA Microarray Data  

E-Print Network [OSTI]

Estimating Dataset Size Requirements for Classifying DNA Microarray Data S. Mukherjee*+#1 , P methodology for estimating dataset size requirements for classifying microarray data using learning curves is introduced. The goal is to use existing classification results to estimate dataset size requirements

Poggio, Tomaso

456

DNA encoding for plant digalactosyldiacylglycerol galactosyltransferase and methods of use  

DOE Patents [OSTI]

The cDNA encoding digalactosyldiacylglycerol galactosyltransferase (DGD1) is provided. The deduced amino acid sequence is also provided. Methods of making and using DGD1 to screen for new herbicides and alter a plant's leaf lipid composition are also provided, as well as expression vectors, transgenic plants or other organisms transfected with said vectors.

Benning, Christoph; Doermann, Peter

2003-11-04T23:59:59.000Z

457

Electronic Signature of DNA Nucleotides via Transverse Transport  

E-Print Network [OSTI]

Electronic Signature of DNA Nucleotides via Transverse Transport Michael Zwolak Physics Department spatial width, each nucleotide carries a unique signature due to the different electronic and chemical structure of the four bases. This signature is independent of the nearest-neighbor nucleotides. Furthermore

Zwolak, Michael

458

Derivatized versions of ligase enzymes for constructing DNA sequences  

DOE Patents [OSTI]

A method of making very long, double-stranded synthetic poly-nucleotides. A multiplicity of short oligonucleotides is provided. The short oligonucleotides are sequentially hybridized to each other. Enzymatic ligation of the oligonucleotides provides a contiguous piece of PCR-ready DNA of predetermined sequence.

Mariella, Jr., Raymond P. (Danville, CA); Christian, Allen T. (Tracy, CA); Tucker, James D. (Novi, MN); Dzenitis, John M. (Livermore, CA); Papavasiliou, Alexandros P. (Oakland, CA)

2006-08-15T23:59:59.000Z

459

OPTIMAL ALGORITHM FOR FINDING DNA MOTIFS WITH NUCLEOTIDE ADJACENT DEPENDENCY  

E-Print Network [OSTI]

OPTIMAL ALGORITHM FOR FINDING DNA MOTIFS WITH NUCLEOTIDE ADJACENT DEPENDENCY FRANCIS Y.L. CHIN that the occurrence of a nucleotide in a binding site is independent of other nucleotides. More complicated representations, such as HMM or regular expression, exist that can capture the nucleotide dependency

Chin, Francis Y.L.

460

The significance of nucleotides within DNA codons: a quantitative approach.  

E-Print Network [OSTI]

The significance of nucleotides within DNA codons: a quantitative approach. Alejandro Guerra amino acids coded by triplets of nucleotides (codons) in the Genetic Code, ap- pears to depend on the nucleotide position within a codon, as well as its physico-chemical features. Although differ- ent orders

Guerra Hern√°ndez, Alejandro

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


461

Nanobiosensors Constraint of DNA on Functionalized Graphene Improves  

E-Print Network [OSTI]

Nanobiosensors Constraint of DNA on Functionalized Graphene Improves its Biostability, Jun Liu, and Yuehe Lin* Graphene, a single-layer carbon crystal, is attracting increasing attention strength, and biocompa- tibility.[1a,c,,2] Recently, functionalized graphene has been successfully used

Aksay, Ilhan A.

462

Exploration and Analysis of DNA Microarray and Protein Array Data  

E-Print Network [OSTI]

genes express in specific cells under specific conditions specific states for cell/organism. Research: Which genes are expressed in which cells and under what conditions. Roadblock: Large number of genes (e by long cDNA probes. For any sample: Spot Intensity ~ gene expression level Two-dye format: Take two m

Cabrera, Javier

463

Efficient Turing-Universal Computation with DNA Polymers  

E-Print Network [OSTI]

of stack machines -- a Turing-universal model of computation similar to Turing machines -- using DNA strand to logically reversible computation, and arbitrarily little energy per computation step is required. Further computation is significantly more efficient than geometry-free chemical reaction networks. 1 Introduction

Winfree, Erik

464

Nature Methods A primer to scaffolded DNA origami  

E-Print Network [OSTI]

Scaffold / staple layout for a tetrameric 60 helix bundle. Supplementary Protocol 1 Step-by-step scaffold production from phage DNA. Supplementary Protocol 2 Setting up folding reactions. Supplementary Protocol 3. Generated with caDNAno v0.2 Nature Methods: doi.10.1038/nmeth.1570 #12;Detailed scaffold / staple lay

Dietz, Hendrik

465

Studies of Enzymes in Mitochondrial DNA Precursor Synthesis  

E-Print Network [OSTI]

. Inherited genetic defects in TK2 have been associated with infantile myopathic form of mtDNA depletion are widely used in anti-viral and anti-cancer chemotherapy, but they can cause severe side-effect such as mt

466

An overview of DNA fingerprinting with sup 32 P nucleotides  

SciTech Connect (OSTI)

The DNA probes radiolabeled with {sup 32}P, a primary tool employed by researchers in the life sciences for > 20 yr, are used by private companies, state-run laboratories, and the FBI to generate autoradiographs displaying the unique banding patterns that constitute the DNA fingerprint. The ability to identify an individual or animal from a biological sample has profound implications. Unidentified bodies, unrecognizable remains, and missing children can be tested and the DNA fingerprint compared to those of family members for positive identification. Paternity can be established before a child's birth. Immigration disputes can easily be resolved. Other uses include pedigree determination and testing for cell-line cross-contamination. Using a DNA fingerprint to determine the guilt or innocence of an individual allegedly involved in a violent crime is very controversial and has great legal and moral implications for society. Forensic laboratories have been challenged to ensure a level of quality control and quality assurance consistent with the weight given to these tests when used as evidence in a court of law.

Pappas, G.G.

1992-01-01T23:59:59.000Z

467

628 A-DNA FRAGMENT d(GGGCGCCC) 251 ' , ....  

E-Print Network [OSTI]

- cooling technique is experimentally very convenient compared to slow cooling over a period of days and.8 and 18.1 A2, respectively. Concluding remarks The successful cooling of several different DNA crystals by different techniques, as demonstrated here and elsewhere (Drew et al., 1982), suggests that cryotechniques

468

Single-DNA Molecule Nanomotor Regulated by Photons  

E-Print Network [OSTI]

of the motor backbone can significantly improve the efficiency of light-to-movement energy conversion of waste. In contrast to multiple-component DNA nanomachines, the intramolecular interaction in this single promising potential for applications which require highly efficient light-driven molecular motors. Nucleic

Tan, Weihong

469

Lightning Induced Arcing an LDRD Report  

SciTech Connect (OSTI)

The purpose of this research was to develop a science-based understanding of the early-time behavior of electric surface arcing in air at atmospheric pressure. As a first step towards accomplishing this, we used a kinetic approach to model an electron swarm as it evolved in a neutral gas under the influence of an applied electric field. A computer code was written in which pseudo-particles, each representing some number of electrons, were accelerated by an electric field. The electric field due to the charged particles was calculated efficiently using a tree algorithm. Collision of the electrons with the background gas led to the creation of new particles through the processes of ionization and photoionization. These processes were accounted for using measured cross-section data and Monte Carlo methods. A dielectric half-space was modeled by imaging the charges in its surface. Secondary electron emission from the surface, resulting in surface charging, was also calculated. Simulation results show the characteristics of a streamer in three dimensions. A numerical instability was encountered before the streamer matured to form branching.

JORGENSON,ROY E.; WARNE,LARRY K.; KUNHARDT,ERICH E.

2000-12-01T23:59:59.000Z

470

7, 50135051, 2007 NO2 from lightning  

E-Print Network [OSTI]

for atmospheric chemistry and climate (WMO, 1999; Intergovernmental Panel on Climate Change, 2001). However, considerable uncertainty remains in the magnitude of this natural5 source of NOx (e.g. Price et al., 1997 advantage over solar occultation in terms of data volume and10 spatial coverage because the latter technique

Paris-Sud XI, Université de

471

Lightning strokes can probe the ionosphere  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmospheric Optical Depth7-1D: VegetationEquipment Surfaces and Interfaces Sample6, 2011 LawrenceEfeedstocksHomesLighting the Way

472

Lightning, atmospheric electricity and climate change  

SciTech Connect (OSTI)

Temperature records indicate that a global warming of 0.5{minus}0.7{degrees}C has occurred over the past century (Hansen and Lebedeff, 1987). Whether this trend is a result of increased trace gas concentrations in the atmosphere, or simply a result of natural variability; is still not known. These temperature trends are derived from thousands of observations worldwide. However, these observations are concentrated largely over continental areas, and then mainly in the northern hemisphere`s populated regions. This northern hemisphere continental bias results in large uncertainties in estimates of global temperature trends. Due to the increasing evidence that the present buildup of greenhouse gases in the atmosphere may result in an additional global warming of 1-5{degrees}C by the year 2050 (IPCC, 1990), it is increasingly important to find afternative methods to monitor fluctuations in global surface temperatures. As shown by two recent studies (Williams, 1992; Price, 1993), the global atmospheric electric circuit may provide a promising afternative for monitoring future climate change.

Price, C.

1993-10-01T23:59:59.000Z

473

Nature Macmillan Publishers Ltd 1998 Structure of the DNA-binding  

E-Print Network [OSTI]

and Jun bound specifically to DNA Lin Chen*, J. N. Mark Glover*, Patrick G. Hogan, Anjana Rao & Stephen C

Glover, Mark

474

A General Method for Quantifying Sequence Effects on Nucleobase Oxidation in DNA  

E-Print Network [OSTI]

Oxidative damage to DNA has long been associated with aging and disease, with guanine serving as the

Margolin, Yelena

2010-01-01T23:59:59.000Z

475

Human Biliverdin Reductase Is a Leucine Zipper-like DNA-binding Protein and Functions in Transcriptional Activation of Heme  

E-Print Network [OSTI]

BVR DNA complex formation; and (e) purified preparations of hBVR or hHO-1 do not bind to DNA with two AP-1

Zulfiqar Ahmad

476

Evaluation of extraction and purification methods for obtaining PCR-amplifiable DNA from compost  

E-Print Network [OSTI]

Evaluation of extraction and purification methods for obtaining PCR-amplifiable DNA from compost complicate the isolation of PCR- amplifiable DNA from compost and other organic-rich samples. In this study from compost decreased with increasing salt concentration in the lysis buffer. DNA purified by gel

Michel Jr., Frederick C.

477

Distance Dependence of Electron Transfer in DNA: The Role of the Reorganization Energy and Free Energy  

E-Print Network [OSTI]

Distance Dependence of Electron Transfer in DNA: The Role of the Reorganization Energy and Free of the solvent reorganization energy and free energy in the heterogeneous DNA environment. DNA is modeled represents water. Model calculations show the importance of including the reorganization energy and the free

Fayer, Michael D.

478

The role of histones and histone modifying enzymes in ribosomal dna silencing in saccharomyces cerevisiae  

E-Print Network [OSTI]

DNA silencing. We found that, unlike Sir2 that represses both Pol II transcription and recombination, histone H1 only represses recombination at the rDNA. The hyperrecombination defect at the rDNA is more severe in sir2? hho1? double mutant than in either single...

Li, Chonghua

2009-05-15T23:59:59.000Z

479

ADENOSINE TRIPHOSPHATE (ATP) AND DEOXYRIBONUCLEIC ACID (DNA) CONTENT OF MARINE MICROALGAE AND BACTERIA WITH  

E-Print Network [OSTI]

ADENOSINE TRIPHOSPHATE (ATP) AND DEOXYRIBONUCLEIC ACID (DNA) CONTENT OF MARINE MICROALGAE the relationship between DNA and ATP content of marine bacteria and microalgae. This relationship was used. Laboratory-derived DNA:ATP ratios ranged from 8.5 to 33 (wt:wt) for cultures of marine microalgae, and from

Luther, Douglas S.

480

DNA nanotubes self-assembled from triple-crossover tiles as templates for conductive nanowires  

E-Print Network [OSTI]

DNA nanotubes self-assembled from triple-crossover tiles as templates for conductive nanowires Dage on the construction and characterization of DNA nanotubes, a self-assembling superstructure composed of DNA tiles plane were used as the basic building blocks. Triple-crossover nanotubes display a constant diameter

Reif, John H.

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


481

Nuclear Assembly with k DNA in Fractionated Xenopus Egg Extracts: An Unexpected Role for Glycogen in  

E-Print Network [OSTI]

Nuclear Assembly with k DNA in Fractionated Xenopus Egg Extracts: An Unexpected Role for Glycogen. Crude extracts of Xenopus eggs are capable of nuclear assembly around chromatin templates or even around protein-free, naked DNA templates. Here the requirements for nuclear assembly around a naked DNA template

Forbes, Douglass

482

DNA binding shifts the redox potential of the transcription factor SoxR  

E-Print Network [OSTI]

DNA binding shifts the redox potential of the transcription factor SoxR Alon A. Gorodetsky , Lars E-modified electrodes are used to probe the effects of binding to DNA on the redox potential of SoxR, a transcription in the absence of DNA. Using Redmond red as a covalently bound redox reporter affixed above the SoxR binding site

Dietrich, Lars

483

Parallel Molecular Computations of Pairwise Exclusive-Or (XOR) Using DNA "String Tile" Self-Assembly  

E-Print Network [OSTI]

Parallel Molecular Computations of Pairwise Exclusive-Or (XOR) Using DNA "String Tile" Self-Assembly of the satisfiability problems. Self-assembly of DNA nanostructures is theoretically an efficient method of executing parallel computation where information is encoded in DNA tiles and a large number of tiles can be self-assembled

Reif, John H.

484

Thesis for the degree of Doctor of Philosophy DNA-Mediated Self-Assembly  

E-Print Network [OSTI]

Thesis for the degree of Doctor of Philosophy DNA-Mediated Self-Assembly of Nanostructures Theory Sundsvall, Sweden 2007 #12;DNA-Mediated Self-Assembly of Nanostructures { Theory and Experiments Bj¨orn H Kopiering Sundsvall, Sweden 2007 #12;Till Joel och Ragnar #12;#12;DNA-Mediated Self-Assembly

Högberg, Björn

485

Self-assembly of three-dimensional prestressed tensegrity structures from DNA  

E-Print Network [OSTI]

Self-assembly of three-dimensional prestressed tensegrity structures from DNA Tim Liedl1,2,3 , Bjo DNA that act as tension-bearing cables. Our DNA ten- segrity structures can self-assemble against of oligodeoxyribonucleotide `staple strands' that self-assemble into tensed structures despite kinetic barriers imposed

Högberg, Björn

486

oligomeric and polymeric DNA Large electrostatic differences in the binding thermodynamics of a cationic peptide to  

E-Print Network [OSTI]

oligomeric and polymeric DNA Large electrostatic differences in the binding thermodynamics electrostatic differences in the binding thermodynamics of a cationic peptide to oligomeric and polymeric DNA binding to polymeric and oligomeric DNA are not equivalent because of long-range electrostatic effects

Lohman, Timothy M.

487

DNA "Nano-Claw": Logic-Based Autonomous Cancer Targeting and Mingxu You,,  

E-Print Network [OSTI]

DNA "Nano-Claw": Logic-Based Autonomous Cancer Targeting and Therapy Mingxu You,, Lu Peng, Na Shao for Research at the Bio/Nano Interface, Shands Cancer Center, UF Genetics Institute, McKnight Brain Institute a DNA- based device, called "Nano-Claw". Combining the special structure-switching properties of DNA

Tan, Weihong

488

SOLVING LARGE DOUBLE DIGESTION PROBLEMS FOR DNA RESTRICTION MAPPING BY USING  

E-Print Network [OSTI]

SOLVING LARGE DOUBLE DIGESTION PROBLEMS FOR DNA RESTRICTION MAPPING BY USING BRANCH;Solving Large Double Digestion Problems for DNA Restriction Mapping by Using Branch-and-Bound Integer.S.A. Abstract. The double digestion problem for DNA restriction mapping has been proved to be NP

489

Characterization of two Bacillus subtilis proteins required for the initiation, restart, and control of DNA replication  

E-Print Network [OSTI]

(cont.) This is consistent with an inability of dnaBS371P cells to adjust the frequency of initiation according to growth rate. I also found that cells over-producing DnaBS371P are filamentous, contain decreased DNA contents, ...

Rokop, Megan E., 1978-

2004-01-01T23:59:59.000Z

490

X-Ray Conformational Study of the DNA Duplex in Solution  

E-Print Network [OSTI]

X-Ray Conformational Study of the DNA Duplex in Solution V. GRASSIAN* and G. W. BRADY,f Rensselaer Earlier x-ray studies on dissolved linear DNA molecules were interpreted on the assumption engaged in x-ray diffraction studies of circular su- perhelical DNA. Some of our preliminary results have

Benham, Craig J.

491

lthough forensic DNA testing is well established, experts sometimes disagree about the interpreta-  

E-Print Network [OSTI]

A lthough forensic DNA testing is well established, experts sometimes disagree about the interpreta. THOMPSON, LAURENCE D. MUELLER, AND DAN E. KRANE 12 Forensic DNA Statistics: Still Controversial In Some Group on DNA Analysis Methods (SWGDAM), a group of forensic scientists chosen by the FBI to propose

Rose, Michael R.

492

Nondetectability of restriction fragments and independence of DNA fragment sizes within and between loci in RFLP typing of DNA  

SciTech Connect (OSTI)

The authors provide experimental evidence showing that, during the restriction-enzyme digestion of DNA samples, some of the HaeIII-digested DNA fragments are small enough to prevent their reliable sizing on a Southern gel. As a result of such nondetectability of DNA fragments, individuals who show a single-band DNA profile at a VNTR locus may not necessarily be true homozygotes. In a population database, when the presence of such nondetectable alleles is ignored, they show that a pseudodependence of alleles within as well as across loci may occur. Using a known statistical method, under the hypothesis of independence of alleles within loci, they derive an efficient estimate of null allele frequency, which may be subsequently used for testing allelic independence within and across loci. The estimates of null allele frequencies, thus derived, are shown to agree with direct experimental data on the frequencies of HaeIII-null alleles. Incorporation of null alleles into the analysis of the forensic VNTR database suggests that the assumptions of allelic independence within and between loci are appropriate. In contrast, a failure to incorporate the occurrence of null alleles would provide a wrong inference regarding the independence of alleles within and between loci. 47 refs., 2 figs., 4 tabs.

Chakraborty, R.; Zhong, Y.; Jin, L. (Univ. of Texas Health Science Center, Houston, TX (United States)); Budowle, B. (FBI Academy, Quantico, VA (United States))

1994-08-01T23:59:59.000Z

493

Probability of double-strand breaks in genome-sized DNA by {gamma}-ray decreases markedly as the DNA concentration increases  

SciTech Connect (OSTI)

By use of the single-molecule observation, we count the number of DNA double-strand breaks caused by {gamma}-ray irradiation with genome-sized DNA molecules (166 kbp). We find that P{sub 1}, the number of double-strand breaks (DSBs) per base pair per unit Gy, is nearly inversely proportional to the DNA concentration above a certain threshold DNA concentration. The inverse relationship implies that the total number of DSBs remains essentially constant. We give a theoretical interpretation of our experimental results in terms of attack of reactive species upon DNA molecules, indicating the significance of the characteristics of genome-sized giant DNA as semiflexible polymers for the efficiency of DSBs.

Shimobayashi, Shunsuke F. [Department of Physics, Graduate School of Science, Kyoto University, Kyoto 606-8502 (Japan); Iwaki, Takafumi [Fukui Institute for Fundamental Chemistry, Kyoto University, Kyoto 606-8103 (Japan); Mori, Toshiaki [Radiation Research Center, Osaka Prefecture University, Sakai 599-8570 (Japan); Yoshikawa, Kenichi [Department of Physics, Graduate School of Science, Kyoto University, Kyoto 606-8502 (Japan); Faculty of Life and Medical Sciences, Doshisha University, Kyoto 610-0394 (Japan)

2013-05-07T23:59:59.000Z

494

Rapid purification of circular DNA by triplex-mediated affinity capture  

DOE Patents [OSTI]

A single-step capture of a target supercoiled double-stranded DNA molecule is accomplished by forming a local triple-helix among two strands of the supercoiled circular DNA and an oligonucleotide probe. The oligonucleotide is bound to an immobilizing support which facilitates the immobilization and purification of target DNA molecules. Non-target DNA molecules and other contaminating cellular material are easily removed by washing. The triple-helical structure is destabilized by raising the pH, leaving purified target DNA in the supernatant and reusable affinity capture oligonucleotide secured to the immobilizing support. 3 figs.

Ji, H.; Smith, L.M.

1997-01-07T23:59:59.000Z

495

A new structural framework for integrating replication protein A into DNA processing machinery  

SciTech Connect (OSTI)

By coupling the protection and organization of ssDNA with the recruitment and alignment of DNA processing factors, Replication Protein A (RPA) lies at the heart of dynamic multi-protein DNA processing machinery. Nevertheless, how RPA manages to coordinate the biochemical functions of its eight domains remains unknown. We examined the structural biochemistry of RPA s DNA binding activity, combining small-angle x-ray and neutron scattering with all-atom molecular dynamics simulations to investigate the architecture of RPA s DNA-binding core. It has been long held that RPA engages ssDNA in three stages, but our data reveal that RPA undergoes two rather than three transitions as it binds ssDNA. In contrast to previous models, RPA is more compact when fully engaged on 20-30 nucleotides of ssDNA than when DNA-free, and there is no evidence for significant population of a highly compacted structure in the initial 8-10 nucleotide binding mode. These results provide a new framework for understanding the integration of ssDNA into DNA processing machinery and how binding partners may manipulate RPA architecture to gain access to the substrate.

Brosey, Chris A [Vanderbilt University; Yan, Chunli [Georgia State University, Atlanta; Tsutakawa, Susan E [Lawrence Berkeley National Laboratory (LBNL); Heller, William T [ORNL; Rambo, Robert P [Lawrence Berkeley National Laboratory (LBNL); Tainer, John A [Lawrence Berkeley National Laboratory, The Scripps Research Institite and The Skaggs Institute; Ivanov, Ivaylo [Georgia State University, Atlanta; Chazin, Walter J [Vanderbilt University

2013-01-01T23:59:59.000Z

496

The Crystal Structure of TAL Effector PthXo1 Bound to Its DNA Target  

SciTech Connect (OSTI)

DNA recognition by TAL effectors is mediated by tandem repeats, each 33 to 35 residues in length, that specify nucleotides via unique repeat-variable diresidues (RVDs). The crystal structure of PthXo1 bound to its DNA target was determined by high-throughput computational structure prediction and validated by heavy-atom derivatization. Each repeat forms a left-handed, two-helix bundle that presents an RVD-containing loop to the DNA. The repeats self-associate to form a right-handed superhelix wrapped around the DNA major groove. The first RVD residue forms a stabilizing contact with the protein backbone, while the second makes a base-specific contact to the DNA sense strand. Two degenerate amino-terminal repeats also interact with the DNA. Containing several RVDs and noncanonical associations, the structure illustrates the basis of TAL effector-DNA recognition.

Mak, Amanda Nga-Sze; Bradley, Philip; Cernadas, Raul A.; Bogdanove, Adam J.; Stoddard, Barry L. (FHCRC); (Iowa State)

2012-02-10T23:59:59.000Z

497

Modulation of DNA loop lifetimes by the free energy of loop formation  

E-Print Network [OSTI]

Storage and retrieval of the genetic information in cells is a dynamic process that requires the DNA to undergo dramatic structural rearrangements. DNA looping is a prominent example of such a structural rearrangement that is essential for transcriptional regulation in both prokaryotes and eukaryotes, and the speed of such regulations affects the fitness of individuals. Here, we examine the in vitro looping dynamics of the classic Lac repressor gene-regulatory motif. We show that both loop association and loop dissociation at the DNA-repressor junctions depend on the elastic deformation of the DNA and protein, and that both looping and unlooping rates approximately scale with the looping J factor, which reflects the system's deformation free energy. We explain this observation by transition state theory and model the DNA-protein complex as an effective worm-like chain with twist. We introduce a finite protein-DNA binding interaction length, in competition with the characteristic DNA deformation length scale, ...

Chen, Yi-Ju; Mulligan, Peter; Spakowitz, Andrew J; Phillips, Rob

2015-01-01T23:59:59.000Z

498

Transverse Electronic Transport through DNA Nucleotides with Functionalized Graphene Electrodes  

E-Print Network [OSTI]

Graphene nanogaps and nanopores show potential for the purpose of electrical DNA sequencing, in particular because single-base resolution appears to be readily achievable. Here, we evaluated from first principles the advantages of a nanogap setup with functionalized graphene edges. To this end, we employed density functional theory and the non-equilibrium Green's function method to investigate the transverse conductance properties of the four nucleotides occurring in DNA when located between the opposing functionalized graphene electrodes. In particular, we determined the electrical tunneling current variation as a function of the applied bias and the associated differential conductance at a voltage which appears suitable to distinguish between the four nucleotides. Intriguingly, we observe for one of the nucleotides a negative differential resistance effect.

Prasongkit, Jariyanee; Pathak, Biswarup; Ahuja, Rajeev; Scheicher, Ralph H

2013-01-01T23:59:59.000Z

499

Effects of Eye-phase in DNA unzipping  

E-Print Network [OSTI]

The onset of an "eye-phase" and its role during the DNA unzipping is studied when a force is applied to the interior of the chain. The directionality of the hydrogen bond introduced here shows oscillations in force-extension curve similar to a "saw-tooth" kind of oscillations seen in the protein unfolding experiments. The effects of intermediates (hairpins) and stacking energies on the melting profile have also been discussed.

Debaprasad Giri; Sanjay Kumar

2007-04-24T23:59:59.000Z

500

Quantum Confinement in Hydrogen Bond of DNA and RNA  

E-Print Network [OSTI]

The hydrogen bond is a fundamental ingredient to stabilize the DNA and RNA macromolecules. The main contribution of this work is to describe quantitatively this interaction as a consequence of the quantum confinement of the hydrogen. The results for the free and confined system are compared with experimental data. The formalism to compute the energy gap of the vibration motion used to identify the spectrum lines is the Variational Method allied to Supersymmetric Quantum Mechanics.

da Silva dos Santos; Elso Drigo Filho; Regina Maria Ricotta

2015-02-09T23:59:59.000Z