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Sample records for dna lightning dock

  1. Geothermal Literature Review At Lightning Dock Geothermal Area...

    Open Energy Info (EERE)

    Geothermal Literature Review At Lightning Dock Geothermal Area (Farhar, 2002) Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Geothermal...

  2. Geothermal Literature Review At Lightning Dock Geothermal Area...

    Open Energy Info (EERE)

    Geothermal Literature Review At Lightning Dock Geothermal Area (Rafferty, 1997) Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Geothermal...

  3. Geothermal Literature Review At Lightning Dock Geothermal Area...

    Open Energy Info (EERE)

    Geothermal Literature Review At Lightning Dock Geothermal Area (Witcher, 2002) Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Geothermal...

  4. Geothermal Literature Review At Lightning Dock Geothermal Area...

    Open Energy Info (EERE)

    Geothermal Literature Review At Lightning Dock Geothermal Area (Sammel, 1978) Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Geothermal...

  5. Geothermal Literature Review At Lightning Dock Geothermal Area...

    Open Energy Info (EERE)

    Geothermal Literature Review At Lightning Dock Geothermal Area (Lienau, 1990) Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Geothermal...

  6. Geothermal Literature Review At Lightning Dock Geothermal Area...

    Open Energy Info (EERE)

    Geothermal Literature Review At Lightning Dock Geothermal Area (Callender, 1981) Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Geothermal...

  7. Geothermal Literature Review At Lightning Dock Geothermal Area...

    Open Energy Info (EERE)

    Geothermal Literature Review At Lightning Dock Geothermal Area (Grant, 1978) Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Geothermal...

  8. Geographic Information System At Lightning Dock Geothermal Area...

    Open Energy Info (EERE)

    Geographic Information System At Lightning Dock Geothermal Area (Getman, 2014) Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Geographic...

  9. Geothermal Literature Review At Lightning Dock Geothermal Area...

    Open Energy Info (EERE)

    Geothermal Literature Review At Lightning Dock Geothermal Area (Smith, 1978) Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Geothermal...

  10. Geothermal Literature Review At Lightning Dock Geothermal Area...

    Open Energy Info (EERE)

    Activity Date Usefulness useful DOE-funding Unknown Exploration Basis Phase 1 study for EGS under a DOE grant Notes A look at EGS potential at Lightning Dock prepared by Lightning...

  11. Thermal Gradient Holes At Lightning Dock Geothermal Area (Cunniff...

    Open Energy Info (EERE)

    Thermal Gradient Holes At Lightning Dock Geothermal Area (Cunniff & Bowers, 2005) Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Thermal...

  12. Geothermal Literature Review At Lightning Dock Geothermal Area...

    Open Energy Info (EERE)

    Geothermal Literature Review At Lightning Dock Geothermal Area (Stone, Et Al., 1977) Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Geothermal...

  13. Geothermal Literature Review At Lightning Dock Geothermal Area...

    Open Energy Info (EERE)

    Geothermal Literature Review At Lightning Dock Geothermal Area (Dahal, Et Al., 2012) Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Geothermal...

  14. Geothermal Literature Review At Lightning Dock Geothermal Area...

    Open Energy Info (EERE)

    At Lightning Dock Geothermal Area (Witcher, Et Al., 2002) Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Geothermal Literature Review At...

  15. Geothermal Literature Review At Lightning Dock Geothermal Area...

    Open Energy Info (EERE)

    Geothermal Literature Review At Lightning Dock Geothermal Area (Elston, Et Al., 1983) Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Geothermal...

  16. Geothermal Literature Review At Lightning Dock Geothermal Area...

    Open Energy Info (EERE)

    Geothermal Literature Review At Lightning Dock Geothermal Area (Clemons, Et Al., 1988) Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Geothermal...

  17. Direct-Current Resistivity Survey At Lightning Dock Area (Cunniff...

    Open Energy Info (EERE)

    Direct-Current Resistivity Survey At Lightning Dock Area (Cunniff & Bowers, 2005) Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Direct-Current...

  18. Direct-Current Resistivity Survey At Lightning Dock Area (Warpinski...

    Open Energy Info (EERE)

    Direct-Current Resistivity Survey At Lightning Dock Area (Warpinski, Et Al., 2002) Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Direct-Current...

  19. Geology and geothermal waters of Lightning Dock region, Animas...

    Open Energy Info (EERE)

    Geology and geothermal waters of Lightning Dock region, Animas Valley and Pyramid Mountains, Hidalgo County, New Mexico Jump to: navigation, search OpenEI Reference LibraryAdd to...

  20. Lightning Dock Geothermal Area | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QAsource History View NewTexas:Montezuma, Arizona: EnergyLebanonTexas:Hill, Texas:Controls/Sensors Jump to:Lightning

  1. Radiometrics At Lightning Dock Geothermal Area (Deal, Et Al., 1978) | Open

    Open Energy Info (EERE)

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  2. Thermal Gradient Holes At Lightning Dock Area (Cunniff & Bowers, 2005) |

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION JEnvironmental Jump to:EA EISTJ AutomationTexas/WindEnergyOpen Energy Information Lightning

  3. Geophysics, Geology and Geothermal Leasing Status of the Lightning...

    Open Energy Info (EERE)

    Leasing Status of the Lightning Dock KGRA, Animas Valley, New Mexico Author C. Smith Published New Mexico Geological Society Guidebook, 1978 DOI Not Provided Check for DOI...

  4. Lightning Dock Geothermal Space Heating Project: Lightning Dock...

    Open Energy Info (EERE)

    home heating systems, which consisted of pumping geothermal water and steam through passive steam heaters, and convert the systems to one using modern heat exchange units. It...

  5. Lightning Dock Geothermal Space Heating Project: Lightning Dock KGRA, New

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIXsource History ViewInformationWinds JumpOxiranchem IncLighthouse SolarBeachHI-01

  6. Lightning Dock Geothermal Area | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QAsource History View NewTexas:Montezuma, Arizona: EnergyLebanonTexas:Hill, Texas:Controls/Sensors Jump

  7. Lightning Dock Geothermal Facility | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIXsource History ViewInformationWinds JumpOxiranchem IncLighthouse SolarBeach

  8. Theory of ball lightning

    E-Print Network [OSTI]

    Wu, H -C

    2014-01-01

    We present a comprehensive explanation on ball lightning, a luminous sphere occasionally witnessed after ordinary lightning. In the last decade, it has been well established that natural lightning routinely generates relativistic electrons, which account for observed x rays. So we assume that, in a ball lightning event, a well-defined relativistic electron bunch is produced by the stepped leader of lightning. When this electron bunch strikes various media, a powerful microwave pulse is emitted by the coherent transition radiation mechanism. This intense microwave ionizes air, evacuates plasmas by its radiation pressure to form a globular plasma cavity, and then gets trapped inside the cavity. This theory successfully explains all characteristics of ball lightning, especially the appearance of ball lightning in fully-screened aircraft. Moreover, the proposed radiation mechanism fully explains the strongest radio signals from lightning and nanosecond spikes in the signals are direct evidences on the generation ...

  9. Well Deepening At Lightning Dock Geothermal Area (Witcher, 2006) | Open

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page| Open Energy Information Serbia-EnhancingEtGeorgia: Energy Resources Jump to:Search Your Data Search PopularEnergy

  10. Water Sampling At Lightning Dock Geothermal Area (Swanberg, 1976) | Open

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page| Open Energy Information Serbia-EnhancingEt Al.,Turin,VillageWarrensource History View NewOpen EnergyEnergyEnergy

  11. Thermal Gradient Holes At Lightning Dock Geothermal Area (Arnold...

    Open Energy Info (EERE)

    Arnold, Anderson, Donaldson, Foster, Gutjahr, Hatton, Hill, Martinez (1978) New Mexico's Energy Resources '77: Office of the State Geologist Additional References Retrieved from...

  12. Geothermal Literature Review At Lightning Dock Geothermal Area...

    Open Energy Info (EERE)

    around the state at over 50 temperature anomalies. Examined correlations between structures and thermal anomalies. Made recommendations for future geothermal use. Much of the...

  13. Compound and Elemental Analysis At Lightning Dock Geothermal...

    Open Energy Info (EERE)

    form View source History View New Pages Recent Changes All Special Pages Semantic SearchQuerying Get Involved Help Apps Datasets Community Login | Sign Up Search Page Edit with...

  14. Geothermal Literature Review At Lightning Dock Geothermal Area...

    Open Energy Info (EERE)

    Area Exploration Technique Geothermal Literature Review Activity Date Usefulness useful DOE-funding Unknown Exploration Basis examining known geothermal sites in New Mexico Notes...

  15. Geothermal Literature Review At Lightning Dock Geothermal Area...

    Open Energy Info (EERE)

    resource assessment. All prior known data was assembled, and work similar to todays GIS analysis was completed using the data. References Stephen Parker, L. Icerman (1988) New...

  16. Geothermal Literature Review At Lightning Dock Geothermal Area...

    Open Energy Info (EERE)

    and correlations were made between the new deep borehole and the nearby 55-7 deep borehole. References J. C. Witcher (2008) Evidence for Large-Scale Laramide Tectonic...

  17. Lightning Dock KGRA, New Mexico's Largest Geothermal Greenhouse...

    Open Energy Info (EERE)

    Largest Geothermal Greenhouse, Largest Aquaculture Facility, and First Binary Electrical Power Plant Jump to: navigation, search OpenEI Reference LibraryAdd to library...

  18. Analytical Modeling At Lightning Dock Geothermal Area (Brook...

    Open Energy Info (EERE)

    Geothermal Area Exploration Technique Analytical Modeling Activity Date 1978 Usefulness useful DOE-funding Unknown Exploration Basis Assessing the accessible energy in known...

  19. Compound and Elemental Analysis At Lightning Dock Area (Norman...

    Open Energy Info (EERE)

    David I. Norman, Joseph Moore (2004) Gas Analysis Of Geothermal Fluid Inclusions- A New Technology For Geothermal Exploration Additional References Retrieved from "http:...

  20. Thermal Gradient Holes At Lightning Dock Geothermal Area (Cunniff...

    Open Energy Info (EERE)

    holes drilled References R.A. Cunniff, R.L. Bowers (2003) Final Report: Enhanced Geothermal Systems Technology Phase II: Animas Valley, New Mexico Additional References...

  1. Geothermal Literature Review At Lightning Dock Geothermal Area...

    Open Energy Info (EERE)

    regional reconnaissance DOE-funding Unknown Exploration Basis studied departures in precipitation patterns Notes This paper primarily focused on precipitation patterns and water...

  2. Ground Gravity Survey At Lightning Dock Geothermal Area (Swanberg, 1976) |

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QAsource History View New PagesSustainableGlynn County,Solar Jump to:ResourcesGriggsOpen| OpenAl., 1979)

  3. Geographic Information System At Lightning Dock Geothermal Area (Getman,

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QAsource History View New PagesSustainable UrbanKentucky: EnergyGateway1997) | Open Energy Information2014) |

  4. Geophysics, Geology and Geothermal Leasing Status of the Lightning Dock

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QAsource History View New PagesSustainable UrbanKentucky: EnergyGateway1997) | OpenRaft riverArea,Information

  5. Geothermal Literature Review At Lightning Dock Geothermal Area (Callender,

    Open Energy Info (EERE)

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  6. Geothermal Literature Review At Lightning Dock Geothermal Area (Dahal, Et

    Open Energy Info (EERE)

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  7. Geothermal Literature Review At Lightning Dock Geothermal Area (Elston, Et

    Open Energy Info (EERE)

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  8. Geothermal Literature Review At Lightning Dock Geothermal Area (Farhar,

    Open Energy Info (EERE)

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  9. Geothermal Literature Review At Lightning Dock Geothermal Area (Fleischman,

    Open Energy Info (EERE)

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  10. Geothermal Literature Review At Lightning Dock Geothermal Area (Grant,

    Open Energy Info (EERE)

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  11. Geothermal Literature Review At Lightning Dock Geothermal Area (Lienau,

    Open Energy Info (EERE)

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  12. Geothermal Literature Review At Lightning Dock Geothermal Area (Parker &

    Open Energy Info (EERE)

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  13. Geothermal Literature Review At Lightning Dock Geothermal Area (Rafferty,

    Open Energy Info (EERE)

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  14. Geothermal Literature Review At Lightning Dock Geothermal Area (Sammel,

    Open Energy Info (EERE)

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  15. Geothermal Literature Review At Lightning Dock Geothermal Area (Schochet,

    Open Energy Info (EERE)

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  16. Geothermal Literature Review At Lightning Dock Geothermal Area (Smith,

    Open Energy Info (EERE)

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  17. Geothermal Literature Review At Lightning Dock Geothermal Area (Spiegel,

    Open Energy Info (EERE)

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  18. Geothermal Literature Review At Lightning Dock Geothermal Area (Stone, Et

    Open Energy Info (EERE)

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  19. Geothermal Literature Review At Lightning Dock Geothermal Area (Summers,

    Open Energy Info (EERE)

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  20. Geothermal Literature Review At Lightning Dock Geothermal Area (Witcher,

    Open Energy Info (EERE)

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  1. Geothermal Literature Review At Lightning Dock Geothermal Area (Witcher,

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QAsource History View New PagesSustainable UrbanKentucky:Bore Technologies IncEnergy2002) | Open1957) |

  2. Geothermal Literature Review At Lightning Dock Geothermal Area (Witcher, Et

    Open Energy Info (EERE)

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  3. Evaluation of Geothermal Potential of Lightning Dock KGRA, New Mexico |

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTIONRobertsdale, AlabamaETEC GmbH JumpEllenville,Power CorpEnergyEunice, Louisiana:PowerOpen Energy

  4. Lightning Dock II Geothermal Project | Open Energy Information

    Open Energy Info (EERE)

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  5. Lightning Dock KGRA, New Mexico's Largest Geothermal Greenhouse, Largest

    Open Energy Info (EERE)

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  6. Isotope Geothermometry At Lightning Dock Geothermal Area (Witcher, 2006) |

    Open Energy Info (EERE)

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  7. Isotopic Analysis- Fluid At Lightning Dock Geothermal Area (Witcher, 2006)

    Open Energy Info (EERE)

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  8. Water Sampling At Lightning Dock Geothermal Area (Witcher, 2006) | Open

    Open Energy Info (EERE)

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  9. Compound and Elemental Analysis At Lightning Dock Geothermal Area

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTIONRobertsdale, Alabama (Utility Company)| Open EnergyColoradoBiomassPlusCompositeJanik,|Al., 2004)

  10. Compound and Elemental Analysis At Lightning Dock Geothermal Area (Elston,

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTIONRobertsdale, Alabama (Utility Company)| Open EnergyColoradoBiomassPlusCompositeJanik,|Al., 2004)Et

  11. Compound and Elemental Analysis At Lightning Dock Geothermal Area (Witcher,

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTIONRobertsdale, Alabama (Utility Company)| Open EnergyColoradoBiomassPlusCompositeJanik,|Al.,

  12. Cation Geothermometers At Lightning Dock Geothermal Area (Witcher, 2006) |

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION JEnvironmentalBowerbank,CammackFLIR Jump to:RAPID RoadmapInformationDM5498 JumpOpen Energy

  13. Geothermal Literature Review At Lightning Dock Geothermal Area (Clemons, Et

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIXsource History View New Pages Recent Changes AllGunneryData | OpenMajer, 2003)

  14. Lightning Dock Geothermal HI-01 | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIXsource History ViewInformationWinds JumpOxiranchem IncLighthouse SolarBeachHI-01 Jump

  15. Lightning Dock I Geothermal Project | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIXsource History ViewInformationWinds JumpOxiranchem IncLighthouse SolarBeachHI-01I

  16. Observation Wells At Lightning Dock Geothermal Area (Reeder, 1957) | Open

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIXsourceII Jump to:Information 3rd|Northfork ElectricName01988) | Open EnergyEnergy

  17. Production Wells At Lightning Dock Geothermal Area (Cyrq Energy, 2014) |

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIXsourceII JumpQuarterly SmartDB-2, BluePoulsenBioSolutions

  18. Eddy Current Model of Ball Lightning

    E-Print Network [OSTI]

    Shelton, J D

    2011-01-01

    Eddy Current Model of Ball Lightning Calculations show that high-energy ball lightning may consist of a ball of plasma containing a large circular electric current arising as an eddy current generated by lightning. Synthetic ball lightning might serve as a method of plasma confinement for purposes of nuclear fusion. In this paper, three articles concerning ball lightning and the related phenomenon of large ball lightning are combined to provide insight into this rarely glimpsed occurrence.

  19. Eddy Current Model of Ball Lightning

    E-Print Network [OSTI]

    J. D. Shelton

    2011-02-07

    Eddy Current Model of Ball Lightning Calculations show that high-energy ball lightning may consist of a ball of plasma containing a large circular electric current arising as an eddy current generated by lightning. Synthetic ball lightning might serve as a method of plasma confinement for purposes of nuclear fusion. In this paper, three articles concerning ball lightning and the related phenomenon of large ball lightning are combined to provide insight into this rarely glimpsed occurrence.

  20. Enlightening lightning! Producing and directing a multimedia planetarium show 

    E-Print Network [OSTI]

    Fowler, Sarah Marie

    2005-02-17

    of storms and lightning. Following is the list of the questions we chose from Understanding Lightning. 1. Why did Benjamin Franklin fly the kite? 2. How does a lightning rod work? 3. How many people are killed by lightning each year? 4. What..., family watching weather and planning hike Lightning mentioned in forecast Why did Benjamin Franklin fly the kite? Elementary Electrostatics Hiking in the park, see lightning damaged tree How does lightning damage trees...

  1. Changes in measured lightning return stroke peak current after the 1994 National Lightning Detection Network upgrade 

    E-Print Network [OSTI]

    Wacker, Robert Scott

    1997-01-01

    Since a comprehensive upgrade of the US National Lightning Detection Network (NLDN) in 1994, the mean peak current of detected cloud-to-ground (CG) lightning flashes has decreased, the number of detected flashes has ...

  2. A comparative analysis of total lightning observations and cloud-to-ground lightning observations in the Southeastern United States region 

    E-Print Network [OSTI]

    Hugo, Keith Michael

    1998-01-01

    A comparison was performed employing lightning data aphics. collected by the Optical Transient Detector (OTD) satellite and the National Lightning Detection Network (NLDN). The feasibility of using total lightning flash data, both intracloud (IC...

  3. Lightning Ball (Ball Lightning) Created by Thunder, Shock-Wave

    E-Print Network [OSTI]

    Domokos Tar

    2010-07-20

    Following his observation the author has described in the papers [1] to [4] the formation of a Lightning Ball (LB)with the help of a new theory of symmetry breaking of the vortex ring. In the frame of this theory he emphasizes the primordial rule of the thunder (shock-wave) during the lightning strike in the creation of the LB. The shock-waves and the sound waves propagate very fast but the subsequent enlargement of the vortex ring (air masses) is very slow. This is the reason why the rotating air ring (cylinder) only appeared some seconds later in the observation because of the great inertia of the system. As a consequence of the stationary Mach shock-waves reflections theory the stable distance of the LB to the ground and its very stable horizontal path, in spite of strong winds and rain can be explained.

  4. Lightning Ball (Ball Lightning) Created by Thunder, Shock-Wave

    E-Print Network [OSTI]

    Tar, Domokos

    2010-01-01

    Following his observation the author has described in the papers [1] to [4] the formation of a Lightning Ball (LB)with the help of a new theory of symmetry breaking of the vortex ring. In the frame of this theory he emphasizes the primordial rule of the thunder (shock-wave) during the lightning strike in the creation of the LB. The shock-waves and the sound waves propagate very fast but the subsequent enlargement of the vortex ring (air masses) is very slow. This is the reason why the rotating air ring (cylinder) only appeared some seconds later in the observation because of the great inertia of the system. As a consequence of the stationary Mach shock-waves reflections theory the stable distance of the LB to the ground and its very stable horizontal path, in spite of strong winds and rain can be explained.

  5. Lightning Safety for You and Your Family

    E-Print Network [OSTI]

    Oklahoma, University of

    information on weather-related deaths to learn how to prevent these tragedies. Many lightning victims say after you hear the last thunder clap. Finally, some victims were struck inside homes or buildings while disabilities. While a small percentage of lightning strike victims die, many survivors must learn to live

  6. Winter 2011 Evaluating Protein-Protein Docking Web Servers

    E-Print Network [OSTI]

    Nina Ly Winter 2011 Evaluating Protein-Protein Docking Web Servers Proteins are involved in many protein docking web servers: PIPER, GRAMM-X, 3D Garden, SmoothDock and PatchDock. I #12;will also perform structure. All of these web servers are freely available with no requirement to have an account

  7. Sandia National Laboratories: Lightning Facility

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power AdministrationRobust, High-ThroughputUpcoming Release of thePrograms: Center forFacebookFactIon BeamLightning

  8. Thermal Gradient Holes At Lightning Dock Area (Warpinski, Et Al., 2004) |

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page| Open Energy Information Serbia-EnhancingEt Al., 2013) |InformationThe2009) | Open Energy2008)| Open EnergyOpen

  9. Thermal Ion Dispersion At Lightning Dock Area (Cunniff & Bowers, 2005) |

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page| Open Energy Information Serbia-EnhancingEt Al., 2013) |InformationThe2009) | Open Energy2008)|Al., 1978)2004)

  10. Surface Gas Sampling At Lightning Dock Area (Norman & Moore, 2004) | Open

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page| Open Energy Information Serbia-Enhancing CapacityVectren)Model for theSunLanSuperDrive IncCity, NewMclaren, 2010)

  11. Surface Gas Sampling At Lightning Dock Area (Norman, Et Al., 2002) | Open

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page| Open Energy Information Serbia-Enhancing CapacityVectren)Model for theSunLanSuperDrive IncCity, NewMclaren,

  12. Ground Gravity Survey At Lightning Dock Area (Cunniff & Bowers, 2005) |

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QAsource History View New PagesSustainableGlynn County,Solar Jump to:ResourcesGriggsOpen| OpenAl., 1979) |OpenOpen

  13. Gamma Log At Lightning Dock Geothermal Area (Witcher, 2006) | Open Energy

    Open Energy Info (EERE)

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  14. Gas Flux Sampling At Lightning Dock Area (Cunniff & Bowers, 2005) | Open

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QAsource History View New PagesSustainable UrbanKentucky: Energy Resources JumpGarfieldGarvin|InformationEnergy

  15. Geology and geothermal waters of Lightning Dock region, Animas Valley and

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QAsource History View New PagesSustainable UrbanKentucky: EnergyGateway1997) | OpenRaft river valley,Pyramid

  16. Direct-Current Resistivity Survey At Lightning Dock Area (Cunniff & Bowers,

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTIONRobertsdale, Alabama (UtilityInstrumentsArea (DOE GTP) JumpDillard(Kauahikaua & Klein,

  17. Numerical Modeling At Lightning Dock Geothermal Area (O'Brien, Et Al.,

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QAsource History ViewMayo, Maryland:NPI VenturesNewSt. Louis, Minnesota:Nulato, Alaska: Energy Resources5)1984) |

  18. Observation Wells At Lightning Dock Area (Warpinski, Et Al., 2004) | Open

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QAsource History ViewMayo, Maryland:NPI VenturesNewSt. Louis,Energy Information Area (Warpinski, Et Al., 2004)

  19. Reflection Survey At Lightning Dock Area (Cunniff & Bowers, 2005) | Open

    Open Energy Info (EERE)

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  20. Stepout-Deepening Wells At Lightning Dock Area (Warpinski, Et Al., 2004) |

    Open Energy Info (EERE)

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  1. Surface Gas Sampling At Lightning Dock Area (Norman & Moore, 2004) | Open

    Open Energy Info (EERE)

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  2. Surface Gas Sampling At Lightning Dock Area (Norman, Et Al., 2002) | Open

    Open Energy Info (EERE)

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  3. Thermal Gradient Holes At Lightning Dock Geothermal Area (Arnold, Et Al.,

    Open Energy Info (EERE)

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  4. Thermal Gradient Holes At Lightning Dock Geothermal Area (Cunniff & Bowers,

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION JEnvironmental Jump to:EA EISTJ AutomationTexas/WindEnergyOpen Energy Information2005) | Open

  5. Thermal Gradient Holes At Lightning Dock Geothermal Area (Cunniff, Et Al.,

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION JEnvironmental Jump to:EA EISTJ AutomationTexas/WindEnergyOpen Energy Information2005) |

  6. Analytical Modeling At Lightning Dock Geothermal Area (Brook, Et Al., 1978)

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION JEnvironmental Jump to:EAandAmminex A S Jump to: navigation,Inof Ground Source Heat| Open Energy

  7. Aeromagnetic Survey At Lightning Dock Area (Warpinski, Et Al., 2002) | Open

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION JEnvironmental Jump to:EAand DaltonSolar EnergyAerodyn Energiesysteme GmbHOpen EnergyEnergy

  8. Flow Test At Lightning Dock Area (Cunniff & Bowers, 2005) | Open Energy

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIX ECoopButtePowerEdistoWhiskey flatsInformationFlint Geothermal Area

  9. Fluid Inclusion Analysis At Lightning Dock Area (Norman & Moore, 2004) |

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIX ECoopButtePowerEdistoWhiskey flatsInformationFlintInformation ) JumpOpen Energy

  10. Ground Gravity Survey At Lightning Dock Area (Warpinski, Et Al., 2002) |

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIXsource History View New PagesInformationEnergy Information 2) JumpHot PotOpen

  11. Aeromagnetic Survey At Lightning Dock Area (Cunniff & Bowers, 2005) | Open

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIX ECoop IncIowaWisconsin: Energy Resources JumpAdelan1986) | Open EnergyEnergy

  12. Compound and Elemental Analysis At Lightning Dock Area (Norman & Moore,

    Open Energy Info (EERE)

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  13. Direct-Current Resistivity Survey At Lightning Dock Area (Warpinski, Et

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIX ECoopButtePower VenturesInformation9)ask queriesWind FarmArea (Thomas, 1986) ||

  14. Production Wells At Lightning Dock Geothermal Area (McCants, 1974) | Open

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIXsourceII JumpQuarterly SmartDB-2, BluePoulsenBioSolutionsEnergy Information McCants,

  15. New mechanism for lightning initiation

    SciTech Connect (OSTI)

    Roussel-Dupre, R.; Buchwald, M.; Gurevich, A. [Lebedev Institute of Physical Sciences, Moscow (Russian Federation)] [and others

    1996-10-01

    This is the final report of a three-year, Laboratory-Directed Research and Development (LDRD) project at the Los Alamos National Laboratory (LANL). To distinguish radio-frequency (rf) signals generated by lightning from the electromagnetic pulse produced by a nuclear explosion, it is necessary to understand the fundamental nature of thunderstorm discharges. The recent debate surrounding the origin of transionospheric pulse pairs (TIPPs) detected by the BLACKBEARD experiment aboard the ALEXIS satellite illustrates this point. We have argued that TIPP events could originate from the upward propagating discharges recently identified by optical images taken from the ground, from airplanes, and from the space shuttle. In addition, the Gamma Ray Observatory (GRO) measurements of x-ray bursts originating from thunderstorms are almost certainly associated with these upward propagating discharges. When taken together, these three measurements point directly to the runaway electron mechanism as the source of the upward discharges. The primary goal of this research effort was to identify the specific role played by the runaway-air-breakdown mechanism in the general area of thunderstorm electricity and in so doing develop lightning models that predict the optical, rf, and x-ray emissions that are observable from space.

  16. Crusader Automated Docking System Phase 3 report

    SciTech Connect (OSTI)

    Jatko, W.B.; Goddard, J.S.; Ferrell, R.K.; Gleason, S.S.; Hicks, J.S.; Varma, V.K.

    1996-03-01

    The US Army is developing the next generation of battlefield artillery vehicles, including an advanced, self-propelled howitzer and a companion resupply vehicle. The resupply vehicle is intended to rendezvous with the howitzer near the battlefront and to upload ammunition to the howitzer. The Army has recommended that the vehicles incorporate robotics to increase safety, by allowing the crew to remain inside their vehicles during resupply operations. Oak Ridge National Laboratory has developed an autonomous docking system for a 6-D.F. robotic, ammunition transfer arm. The docking system augments the operator`s abilities by determining the position and orientation (pose) of a docking port. The pose is the location of the x, y, and z reference axes in 3-D space; and the orientation is the rotations--roll, pitch, and yaw--about those axes. Bye precisely determining the pose of the docking port, the robot can be instructed to move to the docking position without operator intervention. The system uses a video camera and frame grabber to digitize images of the special docking port. Custom algorithms were developed to recognize the port in the camera image, to determine the pose from its image features, and to distribute the results to the robot control computer. The system is loosely coupled to the robot and can be easily adapted to different mechanical configurations. The system has successfully demonstrated autonomous docking on a 24-in. tabletop robot and a 12-ft ammunition resupply robot. The update rate, measurement accuracy, continuous operation, and accuracy with obstructed view have been determined experimentally.

  17. Lightning Strikes on Airborne Grounded Systems 

    E-Print Network [OSTI]

    Malinga, Gilbert Aporu

    2014-10-13

    The modeling of lightning strike behavior and estimation of the subsequent electrical discharge is of great practical importance. In this study a complete two-dimensional physics based analytical formulation is presented ...

  18. A model of the ball lightning

    E-Print Network [OSTI]

    Ignatovich, V K

    1992-01-01

    The ball lightning is supposed to be a shock wave of a point explosion frozen with electrostriction forces of the internal strong laser discharge. The life time of the ball with modest parameters is calculated.

  19. A model of the ball lightning

    E-Print Network [OSTI]

    Vladimir K. Ignatovich

    2011-09-05

    The ball lightning is supposed to be a shock wave of a point explosion frozen with electrostriction forces of the internal strong laser discharge. The life time of the ball with modest parameters is calculated.

  20. Total lightning characteristics of ordinary convection 

    E-Print Network [OSTI]

    Motley, Shane Michael

    2009-06-02

    Twenty-two isolated, non-severe, warm season thunderstorms (ordinary thunderstorms) were examined to test possible correlations between three-dimensional lightning flash characteristics and the complex evolution of the ...

  1. Lightning protection system for a wind turbine

    DOE Patents [OSTI]

    Costin, Daniel P. (Chelsea, VT); Petter, Jeffrey K. (Williston, VT)

    2008-05-27

    In a wind turbine (104, 500, 704) having a plurality of blades (132, 404, 516, 744) and a blade rotor hub (120, 712), a lightning protection system (100, 504, 700) for conducting lightning strikes to any one of the blades and the region surrounding the blade hub along a path around the blade hub and critical components of the wind turbine, such as the generator (112, 716), gearbox (708) and main turbine bearings (176, 724).

  2. Lightning Arrestor Connectors Production Readiness

    SciTech Connect (OSTI)

    Marten, Steve; Linder, Kim; Emmons, Jim; Gomez, Antonio; Hasam, Dawud; Maurer, Michelle

    2008-10-20

    The Lightning Arrestor Connector (LAC), part “M”, presented opportunities to improve the processes used to fabricate LACs. The A## LACs were the first production LACs produced at the KCP, after the product was transferred from Pinnellas. The new LAC relied on the lessons learned from the A## LACs; however, additional improvements were needed to meet the required budget, yield, and schedule requirements. Improvement projects completed since 2001 include Hermetic Connector Sealing Improvement, Contact Assembly molding Improvement, development of a second vendor for LAC shells, general process improvement, tooling improvement, reduction of the LAC production cycle time, and documention of the LAC granule fabrication process. This report summarizes the accomplishments achieved in improving the LAC Production Readiness.

  3. Static electricity measurements for lightning warnings -an exploration

    E-Print Network [OSTI]

    Haak, Hein

    Static electricity measurements for lightning warnings - an exploration H. Bloemink De Bilt, 2013 | Internal report; IR 2013-01 #12;#12;Static electricity measurements for lightning warnings - an exploration Version 1.0 Date January 2013 Status Final #12;#12;Static electricity measurements for lightning warnings

  4. Number of lightning discharges causing damage to lightning arrester cables for aerial transmission lines in power systems

    SciTech Connect (OSTI)

    Nikiforov, E. P. [Electric Power Scientific Research Institute (VNIIE), branch of OAO 'NTTs Elektroenergetika' (Russian Federation)

    2009-07-15

    Damage by lightning discharges to lightning arrester cables for 110-175 kV aerial transmission lines is analyzed using data from power systems on incidents with aerial transmission lines over a ten year operating period (1997-2006). It is found that failures of lightning arrester cables occur when a tensile force acts on a cable heated to the melting point by a lightning current. The lightning currents required to heat a cable to this extent are greater for larger cable cross sections. The probability that a lightning discharge will develop decreases as the amplitude of the lightning current increases, which greatly reduces the number of lightning discharges which damage TK-70 cables compared to TK-50 cables. In order to increase the reliability of lightning arrester cables for 110 kV aerial transmission lines, TK-70 cables should be used in place of TK-50 cables. The number of lightning discharges per year which damage lightning arrester cables is lowered when the density of aerial transmission lines is reduced within the territory of electrical power systems. An approximate relationship between these two parameters is obtained.

  5. 428 IEEE TRANSACTIONS ON ELECTROMAGNETIC COMPATIBILITY, VOL. 51, NO. 3, AUGUST 2009 Overview of Recent Progress in Lightning

    E-Print Network [OSTI]

    Florida, University of

    /lightning locating systems, light- ning electromagnetic pulse and lightning-induced effects, and protection against lightning-induced effects. Index Terms--Lightning discharge, lightning electromagnetic pulse (LEMP electromagnetic pulse (LEMP) and induced effects; 4) protection against LEMP. The first special issue on lightning

  6. Optimum protein-excipient interactions using molecular docking simulations

    E-Print Network [OSTI]

    Tarar, Haider

    2014-04-01

    , two commonly used excipients, the sugars sucrose and mannitol, have been investigated so far. Some of the surfactants which will be looked at as excipients in the future include Octyl glucoside, Tween 40 and Tween 80. Molecular docking... toolkit (http://cactus.nci.nih.gov/translate/). Docking Using ADT (Pokphanh, 2011). Fig. 4: A docking conformation of Octyl Glucoside with Myoglobin in Autodock. Summer 2013 – Spring 2014 | 73 ————————— References Dimitrov Dimiter S., 2012...

  7. Structure of conducting channel of lightning

    SciTech Connect (OSTI)

    Alanakyan, Yu. R.

    2013-08-15

    The spatial distribution of the plasma density in a lightning channel is studied theoretically. It is shown that the electric-field double layer is formed at the channel boundary. In this case, the electron temperature changes abruptly and ions are accelerated by the electric field of the double layer. The ion momentum flux density is close to the surrounding gas pressure. Cleaning of the channel from heavy particles occurs in particle-exchange processes between the plasma channel and the surrounding air. Hydrogen ions are accumulated inside the expanding channel from the surrounding air, which is enriched by hydrogen-contained molecules. In this case, the plasma channel is unstable and splits to a chain of equidistant bunches of plasma. The hydrogen-enrich bunches burn diffusely after recombination exhibiting the bead lightning behavior.

  8. Lightning Strikes and Attribution of Climatic Change

    E-Print Network [OSTI]

    Webster, Anthony J

    2015-01-01

    Using lightning strikes as an example, two possible schemes are discussed for the attribution of changes in event frequency to climate change, and estimating the cost associated with them. The schemes determine the fraction of events that should be attributed to climatic change, and the fraction that should be attributed to natural chance. They both allow for the expected increase in claims and the fluctuations about this expected value. Importantly, the attribution fraction proposed in the second of these schemes is necessarily different to that found in epidemiological studies. This ensures that the statistically expected fraction of attributed claims is correctly equal to the expected increase in claims. The analysis of lightning data highlights two particular difficulties with data-driven, as opposed to modeled, attribution studies. The first is the possibility of unknown "confounding" variables that can influence the strike frequency. This is partly accounted for here by considering the influence of temp...

  9. A new Concept of Ball Lightning

    E-Print Network [OSTI]

    Tsintsadze, Levan N

    2008-01-01

    We suggest that the ball lightning (BL) is a weakly ionized gas, in which the electromagnetic radiation can be accumulated through the Bose-Einstein condensation and/or the photon trapping in the plasma density well. We derive the set of equations describing the stability of BL, and show that the BL moving along charged surface becomes unstable. Eventually the instability leads to explosion of BL and release of energy of the trapped photons and/or the Bose-Einstein condensate.

  10. Docking Unbound Proteins Using Shape Complementarity, Desolvation, and Electrostatics

    E-Print Network [OSTI]

    Weng, Zhiping

    Docking Unbound Proteins Using Shape Complementarity, Desolvation, and Electrostatics Rong Chen1 A comprehensive docking study was performed on 27 distinct protein-protein com- plexes. For 13 test systems space without any knowledge of the binding sites was performed for all proteins except nine antibodies

  11. Robot docking with neural vision and reinforcement Cornelius Weber*,1

    E-Print Network [OSTI]

    Weber, Cornelius

    Robot docking with neural vision and reinforcement Cornelius Weber*,1 , Stefan Wermter, Alexandros, SR6 0DD, UK Available online 10 April 2004 Abstract We present a solution for robotic docking, i.e. approach of a robot toward a table so that it can grasp an object. One constraint is that our People

  12. Autonomous Docking of a Smart Wheelchair for the

    E-Print Network [OSTI]

    Spletzer, John R.

    . From an automation per- spective, autonomously docking the wheelchair onto the lift platform presented platform rails. To solve the docking task, we employed a light detection and ranging (LIDAR)­based approach wheelchair. Van conversions start with a standard van produced by a major automotive manufacturer. The van

  13. Total lightning observations of severe convection over North Texas 

    E-Print Network [OSTI]

    McKinney, Christopher Michael

    2009-05-15

    potential. Total lightning data were obtained from Vaisala Inc.’s Dallas/Fort Worth (D/FW) Lightning Detection and Ranging (LDAR) network. Radar data from two Weather Surveillance Radar – 1988 Doppler (WSR-88D) sites were used for position data...

  14. LIGHTNING EXPOSURE OF WIND TURBINES University of Toronto

    E-Print Network [OSTI]

    Lehn, Peter W.

    LIGHTNING EXPOSURE OF WIND TURBINES Dale Dolan University of Toronto e-mail: dale@ecf.utoronto.ca Abstract This paper applies the electrogeometric model of lightning exposure to a wind turbine to compute. For a typical 45 m wind turbine, the probability of being struck by a downward negative flash, as predicted

  15. Broadband VLF measurements of lightning-induced ionospheric perturbations

    E-Print Network [OSTI]

    Cummer, Steven A.

    2005. [1] Very low frequency (VLF) electromagnetic pulses radiated by lightning are an effective tool with the theoretically predicted ionization changes produced directly by the lightning electromagnetic pulse. Citation over the thunderstorm due to the heating of ionospheric electrons by the electromagnetic pulse (EMP

  16. 2006 ILDC/ILMC Validation of Operational LDS VALIDATION OF AN OPERATIONAL LIGHTNING DETECTION SYSTEM

    E-Print Network [OSTI]

    Haak, Hein

    lightning detection system. Since 1995 KNMI operates a SAFIR (Surveillance et Alerte Foudre par of the lightning data of the SAFIR system. Validation against other operational lightning detection systems2006 ILDC/ILMC Validation of Operational LDS VALIDATION OF AN OPERATIONAL LIGHTNING DETECTION

  17. An improved model of the lightning electromagnetic field interaction with the D-region ionosphere

    E-Print Network [OSTI]

    14 March 2012. [1] We present an improved time-domain model of the lightning electromagnetic pulse. Introduction [2] Lightning discharges produce both an electromagnetic pulse (EMP), due to the rapid lightningAn improved model of the lightning electromagnetic field interaction with the D-region ionosphere R

  18. Observation of Lightning Ball (Ball Lightning): A new phenomenological description of the phenomenon

    E-Print Network [OSTI]

    Tar, Domokos

    2009-01-01

    The author (physicist)has observed the very strange,beautiful and frightening Lightning Ball (LB). He has never forgotten this phenomenon. During his working life he could not devote himself to the problem of LB-formation.Only two years ago as he has been reading different unbelievable models of LB-formation, he decided to work on this problem. By studying the literature and the crucial points of his observation the author succeeded in creating a completely new model of Lightning Ball(LB) and Ball Lightning(BL)-formation based on the symmetry breaking of the hydrodynamic vortex ring.This agrees fully with the observation and overcomes the shortcomings of current models of LB formation. This model provides answers to the questions: Why are LBs so rarely observed,why do BLs in rare cases have such a high energy and how can we generate LB in the laboratory? Moreover the author differentiates between LB and BL, the latter having a high energy and occuring in 5 % of the observations. Keywords: ball lightning, hydr...

  19. Observation of Lightning Ball (Ball Lightning): A new phenomenological description of the phenomenon

    E-Print Network [OSTI]

    Domokos Tar

    2009-10-05

    The author (physicist)has observed the very strange,beautiful and frightening Lightning Ball (LB). He has never forgotten this phenomenon. During his working life he could not devote himself to the problem of LB-formation.Only two years ago as he has been reading different unbelievable models of LB-formation, he decided to work on this problem. By studying the literature and the crucial points of his observation the author succeeded in creating a completely new model of Lightning Ball(LB) and Ball Lightning(BL)-formation based on the symmetry breaking of the hydrodynamic vortex ring.This agrees fully with the observation and overcomes the shortcomings of current models of LB formation. This model provides answers to the questions: Why are LBs so rarely observed,why do BLs in rare cases have such a high energy and how can we generate LB in the laboratory? Moreover the author differentiates between LB and BL, the latter having a high energy and occuring in 5 % of the observations. Keywords: ball lightning, hydrodynamic vortex ring, symmetry breaking, electroluminescence, triboelectrification.

  20. A Docking Casette For Printed Circuit Boards

    DOE Patents [OSTI]

    Barringer, Dennis R. (Wallkill, NY); Seminaro, Edward J. (Milton, NY); Toffler, Harold M. (Newburgh, NY)

    2003-08-19

    A docking apparatus for printed circuit boards including a cassette housing, having a housing base, a housing cover and a housing wall, wherein the housing base and the housing wall are disposed relative to each other so as to define a housing cavity for containing a printed circuit board and wherein the housing wall includes a cable opening disposed so as to be communicated with the housing cavity, a linkage mechanism, wherein the linkage mechanism includes an engagement configuration and a disengagement configuration and wherein the linkage mechanism is disposed so as to be associated with the cassette housing and a housing bezel, wherein the housing bezel is disposed relative to the cassette housing so as to be associated with the cable opening.

  1. Lightning strokes can probe the ionosphere

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home Room NewsInformationJesse BergkampCenter (LMI-EFRC) ProximityCenter (LMI-EFRC)LightingLightning

  2. Lightning strokes can probe the ionosphere

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration would likeUniverseIMPACTThousand CubicResourcelogo and-E C H2015 Non-ResidentialLightning

  3. A genetic algorithm based method for docking flexible molecules

    SciTech Connect (OSTI)

    Judson, R.S. [Sandia National Labs., Livermore, CA (United States); Jaeger, E.P.; Treasurywala, A.M. [Sterling-Winthrop Inc., Collegeville, PA (United States)

    1993-11-01

    The authors describe a computational method for docking flexible molecules into protein binding sites. The method uses a genetic algorithm (GA) to search the combined conformation/orientation space of the molecule to find low energy conformation. Several techniques are described that increase the efficiency of the basic search method. These include the use of several interacting GA subpopulations or niches; the use of a growing algorithm that initially docks only a small part of the molecule; and the use of gradient minimization during the search. To illustrate the method, they dock Cbz-GlyP-Leu-Leu (ZGLL) into thermolysin. This system was chosen because a well refined crystal structure is available and because another docking method had previously been tested on this system. Their method is able to find conformations that lie physically close to and in some cases lower in energy than the crystal conformation in reasonable periods of time on readily available hardware.

  4. Global reactive nitrogen deposition from lightning NOx A. Shepon,1

    E-Print Network [OSTI]

    Gildor, Hezi

    ., 2002]. The anthropogenic perturbation to the nitrogen cycle (i.e., the increased fossil fuel usage of lightning activity [Price and Rind, 1994; Reeve and Toumi, 1999], creating more available nitrogen compounds

  5. Sandia Lightning Early Warning Network: Digital-based upgrade

    SciTech Connect (OSTI)

    Boyd, G.M.

    1994-05-01

    This report describes the layout and operation of the recently upgraded Sandia Lightning Early Warning Network, which was upgraded from an analog-based to a digital-based telemetry system.

  6. Radar Nowcasting of Total Lightning over the Kennedy Space Center 

    E-Print Network [OSTI]

    Seroka, Gregory Nicholas

    2011-08-08

    intracloud (IC) and/or total (IC + CG) lightning. In addition to CG lightning, IC flashes are of great concern to KSC launch operations. Four years (2006-2009) of summer (June, July, August) daytime (about 14-00 Z) Weather Surveillance Radar ? 1988... Page 1 Florida flash density from 1986-95. ......................................................... 3 2 LDAR-I and LDAR-II site locations ........................................................ 13 3 Various estimates of LDAR-I flash...

  7. Ball lightning observation: an objective video-camera analysis report

    E-Print Network [OSTI]

    Sello, Stefano; Paganini, Enrico

    2011-01-01

    In this paper we describe a video-camera recording of a (probable) ball lightning event and both the related image and signal analyses for its photometric and dynamical characterization. The results strongly support the BL nature of the recorded luminous ball object and allow the researchers to have an objective and unique video document of a possible BL event for further analyses. Some general evaluations of the obtained results considering the proposed ball lightning models conclude the paper.

  8. Ball lightning observation: an objective video-camera analysis report

    E-Print Network [OSTI]

    Stefano Sello; Paolo Viviani; Enrico Paganini

    2011-02-18

    In this paper we describe a video-camera recording of a (probable) ball lightning event and both the related image and signal analyses for its photometric and dynamical characterization. The results strongly support the BL nature of the recorded luminous ball object and allow the researchers to have an objective and unique video document of a possible BL event for further analyses. Some general evaluations of the obtained results considering the proposed ball lightning models conclude the paper.

  9. X-ray Emission from Thunderstorms and Lightning

    ScienceCinema (OSTI)

    Dwyer, Joseph [Florida Institute of Technology, Melbourne, Florida, United States

    2010-01-08

    How lightning is initiated in the relatively low electric fields inside thunderclouds and how it can then propagate for tens of kilometers through virgin air are two of the great unsolved problems in the atmospheric sciences.  Until very recently it was believed that lightning was entirely a conventional discharge, involving only low-energy (a few eV) electrons.  This picture changed completely a few years ago with the discovery of intense x-ray emission from both natural cloud-to-ground lightning and rocket-triggered lightning.  This energetic emission cannot be produced by a conventional discharge, and so the presence of x-rays strongly implies that runaway breakdown plays a role in lightning processes.  During runaway breakdown, electrons are accelerated through air to nearly the speed of light by strong electric fields.  These runaway electrons then emit bremsstrahlung x-rays and gamma-rays during collisions with air.  Indeed, the x-ray and gamma-ray emission produced by runaway breakdown near the tops of thunderstorms is bright enough to be seen from outer space, 600 km away.  As a result, the physics used for decades to describe thunderstorm electrification and lightning discharges is incomplete and needs to be revisited. 

  10. Variability of CONUS Lightning in 2003–12 and Associated Impacts

    E-Print Network [OSTI]

    Koshak, William J.

    Changes in lightning characteristics over the conterminous United States (CONUS) are examined to support the National Climate Assessment (NCA) program. Details of the variability of cloud-to-ground (CG) lightning characteristics ...

  11. Thunderstorm lightning and radar characteristics: insights on electrification and severe weather forecasting 

    E-Print Network [OSTI]

    Steiger, Scott Michael

    2007-04-25

    Total lightning mapping, along with radar and NLDN cloud-to-ground lightning data, can be used to diagnose the severity of a storm. Analysis of the 13 October 2001 supercell event (Dallas-Fort Worth, Texas), some supercells ...

  12. Automated Spacecraft Docking Using a Vision-Based Relative Navigation Sensor 

    E-Print Network [OSTI]

    Morris, Jeffery C.

    2010-01-14

    duration human missions than otherwise possible and could even accelerate human colonization of other worlds. This thesis develops an optimal docking controller for an automated docking capable spacecraft. An innovative vision-based relative navigation...

  13. The Morphology of Eyewall Lightning Outbreaks in Two Category 5 Hurricanes* K. SQUIRES AND S. BUSINGER

    E-Print Network [OSTI]

    Businger, Steven

    The Morphology of Eyewall Lightning Outbreaks in Two Category 5 Hurricanes* K. SQUIRES AND S of lightning outbreaks in the eyewalls of Hurricanes Rita and Katrina, two of the strongest storms in the Atlantic hurricane record. Each hurricane produced eyewall lightning outbreaks during the period of most

  14. ProteinProtein Docking: Overview and Performance Kevin Wiehe, Matthew W. Peterson, Brian Pierce, Julian Mintseris,

    E-Print Network [OSTI]

    Weng, Zhiping

    ­protein docking with specific emphasis on our Fast Fourier Transform-based rigid-body docking program ZDOCK, which is consistently rated as one of the most accurate docking programs in the Critical Assessment of Predicted Structure Prediction, Second Edition Edited by: M. Zaki and C. Bystroff © Humana Press Inc., Totowa, NJ 283

  15. An Integrated Docking Pipeline for the Prediction of Large-Scale Protein-Protein Interactions

    E-Print Network [OSTI]

    An Integrated Docking Pipeline for the Prediction of Large-Scale Protein-Protein Interactions Xin. In this study, we developed a protein-protein docking pipeline (PPDP) that integrates a variety of state studies. In this study, we developed a protein-protein docking pipeline by integrat

  16. Docking Task for Nonholonomic Mobile Robots Olivier Lefebvre, Florent Lamiraux

    E-Print Network [OSTI]

    Lamiraux, Florent

    in a realistic scenario. WareHouse unloading platform Fig. 1. A docking task for a truck: The final configuration is defined relatively to the unloading platform and to the white lines on the ground. The truck must move configuration must be defined relatively to the environment. For instance, one can define a car parking lot as

  17. Cognitive Vision for Autonomous Satellite Rendezvous and Docking

    E-Print Network [OSTI]

    Terzopoulos, Demetri

    with the challenge of effec- tively combining low-level vision with artificial intelli- gence. Some of the earliestCognitive Vision for Autonomous Satellite Rendezvous and Docking Faisal Z. Qureshi and Demetri MDRobotics Limited, Brampton, ON L6S 4J3, Canada Abstract We present a cognitively-controlled vision system

  18. Final Project Docking Algorithms of Virtual Ligand Screening

    E-Print Network [OSTI]

    publicly available; many of these structures are potential drug targets. Because of recent advances the protein surface, choosing or building a library of ligands, flexibly docking those ligands, scoring algorithms and often combine several of various scoring functions. Though there are many examples in which

  19. A possible relationship between Global Warming and Lightning Activity in India during the period 1998-2009

    E-Print Network [OSTI]

    Felix Pereira B.; Priyadarsini G.; T. E. Girish

    2010-12-15

    Lightning activity on a global scale has been studied season wise using satellite data for the period from 1998 to 2009. Lightning activity shows an increasing trend during the period of study which is highly correlated with atmospheric warming. A similar increasing trend of lightning activity is observed in the Indian region during the pre-monsoon season which is correlated with global lightning trends and warming trends of surface temperature in India. Key words: Global warming, lightning activity, Solar cycle changes

  20. Carbon dioxide emission during forest fires ignited by lightning

    E-Print Network [OSTI]

    Pelc, Magdalena

    2009-01-01

    In this paper we developed the model for the carbon dioxide emission from forest fire. The master equation for the spreading of the carbon dioxide to atmosphere is the hyperbolic diffusion equation. In the paper we study forest fire ignited by lightning. In that case the fores fire has the well defined front which propagates with finite velocity.

  1. Carbon dioxide emission during forest fires ignited by lightning

    E-Print Network [OSTI]

    Magdalena Pelc; Radoslaw Osuch

    2009-03-31

    In this paper we developed the model for the carbon dioxide emission from forest fire. The master equation for the spreading of the carbon dioxide to atmosphere is the hyperbolic diffusion equation. In the paper we study forest fire ignited by lightning. In that case the fores fire has the well defined front which propagates with finite velocity.

  2. On the transmission line model for lightning return stroke representation

    E-Print Network [OSTI]

    Florida, University of

    [Uman and McLain, 1969] has been widely used for lightning electromagnetic pulse (LEMP) calculations of current sources that produce a spherical transverse electromagnetic (TEM) wave in the case of return electromagnetic field structure is non-TEM. INDEX TERMS: 0619 Electromagnetics: Electromagnetic theory; 0684

  3. Characterization of infrasound from lightning J. D. Assink,1

    E-Print Network [OSTI]

    Utrecht, Universiteit

    , high-altitude discharges [Farges et al., 2005; Liszka and Hobara, 2006]. This article will discuss CC] During thunderstorm activity in the Netherlands, electromagnetic and infrasonic signals are emitted due a electromagnetic lightning detection network is successful up to distances of 50 km from the infrasound array

  4. The search for Titan lightning radio emissions G. Fischer1

    E-Print Network [OSTI]

    Gurnett, Donald A.

    the existence of Titan lightning were found. Great care is needed in the interpretation of bursty signals since al. [2006] found no thunder clap recorded by the acoustic sensor and reported the observation of an electric signal at 36 Hz throughout the descent. However, it could not be confirmed if this emission

  5. PRECIPITATION OF RADIATION BELT ELECTRONS BY LIGHTNING-GENERATED

    E-Print Network [OSTI]

    PRECIPITATION OF RADIATION BELT ELECTRONS BY LIGHTNING-GENERATED MAGNETOSPHERICALLY REFLECTING-order model to estimate the L-shell dependence of the precipitation flux of energetic electrons driven by MR L-shell extent) peak in the precipitation signature can result due to the focusing of the whistler

  6. Update Direct-Strike Lightning Environment for Stockpile-to-Target Sequence: Supplement LLNL Subcontract #B568621 Lightning Protection at the Yucca Mountain Waste Storage Facility

    SciTech Connect (OSTI)

    Uman, M A

    2008-10-09

    The University of Florida has surveyed all relevant publications reporting lightning damage to metals, metals which could be used as components of storage containers for nuclear waste materials. We show that even the most severe lightning could not penetrate the stainless steel thicknesses proposed for nuclear waste storage casks.

  7. Effects of protein conformation in docking: improved pose prediction through protein pocket adaptation

    E-Print Network [OSTI]

    Jain, Ajay N.

    2009-01-01

    in docking: improved pose prediction through protein pocketacceptable results in pose prediction have been generallyoptimization requires accurate pose prediction for novel

  8. Ball lightning as a possible manifestation of high-temperature superconductivity in Nature

    E-Print Network [OSTI]

    B. L. Birbrair

    2001-02-06

    In the superconducting medium the circular current supported by its own magnetic field can exist giving rise to the possible underlying mechanism for the ball lightning.

  9. Ball lightning as a possible manifestation of high-temperature superconductivity in Nature

    E-Print Network [OSTI]

    Birbrair, B L

    2001-01-01

    In the superconducting medium the circular current supported by its own magnetic field can exist giving rise to the possible underlying mechanism for the ball lightning.

  10. Nanotechnology with DNA DNA Nanodevices

    E-Print Network [OSTI]

    Ludwig-Maximilians-Universität, München

    Nanotechnology with DNA DNA Nanodevices Friedrich C. Simmel* and Wendy U. Dittmer A DNA actuator. Introduction.............285 2. Overview: DNA Nanotechnology.......285 3. Prototypes of Nanomechanical DNA overview of DNA nanotechnology as a whole is given. The most important properties of DNA molecules

  11. Climatological lightning characteristics of the Southern Rocky and Appalachian Mountain chains, a comparison of two distinct mountain effects 

    E-Print Network [OSTI]

    Phillips, Stephen Edward

    2001-01-01

    This study presents a high-resolution lightning climatology for southern portions of both the Rocky Mountains and the Appalachian Mountains. Data from the National Lightning Detection Network (NLDN) are analyzed to produce maps of average annual...

  12. Correction of Lightning Effects on Water Content Reflectometer Soil Moisture Data John S. McCartney* and Jorge G. Zornberg

    E-Print Network [OSTI]

    Zornberg, Jorge G.

    Correction of Lightning Effects on Water Content Reflectometer Soil Moisture Data John S. Mc content measured using WCR (water content reflectometer) probes are susceptible to lightning. Several lightning strikes at the site caused unrealistic shifts in the inferred moisture content (from 0

  13. DNA Mixture DNA Mixture

    E-Print Network [OSTI]

    ;Introductions J.M. Butler ­ Wisconsin DNA Mixture Training May 12, 2009 http://www.cstl.nist.gov/biotech.M. Butler ­ Wisconsin DNA Mixture Training May 12, 2009 http://www.cstl.nist.gov/biotech textbook (now in its 2nd Edition) · STRBase website: http://www.cstl.nist.gov/biotech/strbase/ · Family

  14. DOCK2 regulates cell proliferation through Rac and ERK activation in B cell lymphoma

    SciTech Connect (OSTI)

    Wang, Lei [Laboratory of Cancer Research, Department of Pathology, Hokkaido University Graduate School of Medicine, N15W7, Kitaku, Sapporo 060-8638 (Japan)] [Laboratory of Cancer Research, Department of Pathology, Hokkaido University Graduate School of Medicine, N15W7, Kitaku, Sapporo 060-8638 (Japan); Nishihara, Hiroshi, E-mail: nisihara@patho2.med.hokudai.ac.jp [Laboratory of Translational Pathology, Department of Pathology, Hokkaido University Graduate School of Medicine, N15W7, Kitaku, Sapporo 060-8638 (Japan)] [Laboratory of Translational Pathology, Department of Pathology, Hokkaido University Graduate School of Medicine, N15W7, Kitaku, Sapporo 060-8638 (Japan); Kimura, Taichi; Kato, Yasutaka; Tanino, Mishie [Laboratory of Cancer Research, Department of Pathology, Hokkaido University Graduate School of Medicine, N15W7, Kitaku, Sapporo 060-8638 (Japan)] [Laboratory of Cancer Research, Department of Pathology, Hokkaido University Graduate School of Medicine, N15W7, Kitaku, Sapporo 060-8638 (Japan); Nishio, Mitsufumi; Obara, Masato; Endo, Tomoyuki; Koike, Takao [Department of Medicine II, Hokkaido University Graduate School of Medicine, N15W7, Kitaku, Sapporo 060-8638 (Japan)] [Department of Medicine II, Hokkaido University Graduate School of Medicine, N15W7, Kitaku, Sapporo 060-8638 (Japan); Tanaka, Shinya [Laboratory of Cancer Research, Department of Pathology, Hokkaido University Graduate School of Medicine, N15W7, Kitaku, Sapporo 060-8638 (Japan)] [Laboratory of Cancer Research, Department of Pathology, Hokkaido University Graduate School of Medicine, N15W7, Kitaku, Sapporo 060-8638 (Japan)

    2010-04-23

    DOCK2; a member of the CDM protein family, regulates cell motility and cytokine production through the activation of Rac in mammalian hematopoietic cells and plays a pivotal role in the modulation of the immune system. Here we demonstrated the alternative function of DOCK2 in hematopoietic tumor cells, especially in terms of its association with the tumor progression. Immunostaining for DOCK2 in 20 cases of human B cell lymphoma tissue specimens including diffuse large B cell lymphoma and follicular lymphoma revealed the prominent expression of DOCK2 in all of the lymphoma cells. DOCK2-knockdown (KD) of the B cell lymphoma cell lines, Ramos and Raji, using the lentiviral shRNA system presented decreased cell proliferation compared to the control cells. Furthermore, the tumor formation of DOCK2-KD Ramos cell in nude mice was significantly abrogated. Western blotting analysis and pull-down assay using GST-PAK-RBD kimeric protein suggested the presence of DOCK2-Rac-ERK pathway regulating the cell proliferation of these lymphoma cells. This is the first report to clarify the prominent role of DOCK2 in hematopoietic malignancy.

  15. AutoDock 3.0 SOFTWARE LICENSE AGREEMENT FOR ACADEMIC, NOT FOR PROFIT USE ONLY

    E-Print Network [OSTI]

    AutoDock 3.0 SOFTWARE LICENSE AGREEMENT FOR ACADEMIC, NOT FOR PROFIT USE ONLY Material, of these materials. · You understand that no other right or license to this program or its derivatives is granted.J. (1998) Automated docking using Lamarckian genetic algorithm and an empirical binding free energy

  16. PIPER: An FFT-Based Protein Docking Program with Pairwise Potentials

    E-Print Network [OSTI]

    Vajda, Sandor

    Stephen R. Comeau,2 and Sandor Vajda1,2* 1 Department of Biomedical Engineering, Boston University, Boston that sub- stantially improve the docking results. The basic idea is approximating the interaction matrix­protein docking is to determine the structure of a complex in atomic detail, starting from the coordinates

  17. A stepped leader model for lightning including charge distribution in branched channels

    SciTech Connect (OSTI)

    Shi, Wei; Zhang, Li [School of Electrical Engineering, Shandong University, Jinan 250061 (China); Li, Qingmin, E-mail: lqmeee@ncepu.edu.cn [Beijing Key Lab of HV and EMC, North China Electric Power University, Beijing 102206 (China); State Key Lab of Alternate Electrical Power System with Renewable Energy Sources, Beijing 102206 (China)

    2014-09-14

    The stepped leader process in negative cloud-to-ground lightning plays a vital role in lightning protection analysis. As lightning discharge usually presents significant branched or tortuous channels, the charge distribution along the branched channels and the stochastic feature of stepped leader propagation were investigated in this paper. The charge density along the leader channel and the charge in the leader tip for each lightning branch were approximated by introducing branch correlation coefficients. In combination with geometric characteristics of natural lightning discharge, a stochastic stepped leader propagation model was presented based on the fractal theory. By comparing simulation results with the statistics of natural lightning discharges, it was found that the fractal dimension of lightning trajectory in simulation was in the range of that observed in nature and the calculation results of electric field at ground level were in good agreement with the measurements of a negative flash, which shows the validity of this proposed model. Furthermore, a new equation to estimate the lightning striking distance to flat ground was suggested based on the present model. The striking distance obtained by this new equation is smaller than the value estimated by previous equations, which indicates that the traditional equations may somewhat overestimate the attractive effect of the ground.

  18. TRMM observations of the global relationship between ice water content and lightning

    E-Print Network [OSTI]

    Rutledge, Steven

    TRMM observations of the global relationship between ice water content and lightning Walter A to retrieve ice water content. Citation: Petersen, W. A., H. J. Christian, and S. A. Rutledge (2005), TRMM observations of the global relationship between ice water content and lightning, Geophys. Res. Lett., 32, L

  19. Lightning-Driven Electric and Magnetic Fields Measured in the Stratosphere: Implications for Sprites

    E-Print Network [OSTI]

    Thomas, Jeremy N.

    Lightning-Driven Electric and Magnetic Fields Measured in the Stratosphere: Implications-Driven Electric and Magnetic Fields Measured in the Stratosphere: Implications for Sprites Jeremy Norman Thomas et al., 2004b], in which the lightning-driven electric and magnetic field changes were rare, while

  20. Cloud-to-Ground Lightning Downwind of the 2002 Hayman Forest Fire in Colorado

    E-Print Network [OSTI]

    Rutledge, Steven

    Cloud-to-Ground Lightning Downwind of the 2002 Hayman Forest Fire in Colorado Timothy J. Lang@atmos.colostate.edu 1 #12;Abstract The Hayman forest fire occurred near Denver, burning ~138,000 acres during 8 June-2 ingesting smoke from forest fires. For example, Latham (1991) observed entirely +CG lightning from

  1. A ground level gamma-ray burst observed in association with rocket-triggered lightning

    E-Print Network [OSTI]

    Florida, University of

    A ground level gamma-ray burst observed in association with rocket-triggered lightning J. R. Dwyer 2004; published 13 March 2004. [1] We report the observation of an intense gamma-ray burst observed lightning channel with gamma-ray energies extending up to more than 10 MeV. The burst consisted of 227

  2. Source altitudes of terrestrial gamma-ray flashes produced by lightning leaders

    E-Print Network [OSTI]

    Pasko, Victor

    ; published 18 April 2012. [1] Terrestrial gamma-ray flashes (TGFs) are energetic photon bursts observed fromSource altitudes of terrestrial gamma-ray flashes produced by lightning leaders Wei Xu,1 Sebastien. Pasko (2012), Source altitudes of terres- trial gamma-ray flashes produced by lightning leaders, Geophys

  3. Broadband very low frequency measurement of D region ionospheric perturbations caused by lightning electromagnetic pulses

    E-Print Network [OSTI]

    Cummer, Steven A.

    electromagnetic pulses Zhenggang Cheng,1 Steven A. Cummer,1 Han-Tzong Su,2 and Rue-Ron Hsu2 Received 10 May 2006 the lightning electromagnetic pulse (EMP) associated with elves or from quasielectrostatic fields associated perturbations caused by lightning electromagnetic pulses, J. Geophys. Res., 112, A06318, doi:10.1029/2006JA

  4. Temporal-spatial modeling of electron density enhancement due to successive lightning strokes

    E-Print Network [OSTI]

    Otago, University of

    (FDTD) model that describes the effect of lightning electromagnetic pulses (EMP) on the ionosphere. Each The electromagnetic pulse (EMP) from lightning has been shown to modify conductivity and electron density in the lower successive EMP pulse interacts with a modified background ionosphere due to the previous pulses, resulting

  5. Update Direct-Strike Lightning Environment for Stockpile-to-Target Sequence

    SciTech Connect (OSTI)

    Uman, M A; Rakov, V A; Elisme, J O; Jordan, D M; Biagi, C J; Hill, J D

    2008-10-01

    The University of Florida has surveyed all relevant publications reporting lightning characteristics and presents here an up-to-date version of the direct-strike lightning environment specifications for nuclear weapons published in 1989 by R. J. Fisher and M. A. Uman. Further, we present functional expressions for current vs. time, current derivative vs. time, second current derivative vs. time, charge transfer vs. time, and action integral (specific energy) vs. time for first return strokes, for subsequent return strokes, and for continuing currents; and we give sets of constants for these expressions so that they yield approximately the median and extreme negative lightning parameters presented in this report. Expressions for the median negative lightning waveforms are plotted. Finally, we provide information on direct-strike lightning damage to metals such as stainless steel, which could be used as components of storage containers for nuclear waste materials; and we describe UF's new experimental research program to add to the sparse data base on the properties of positive lightning. Our literature survey, referred to above, is included in four Appendices. The following four sections (II, III, IV, and V) of this final report deal with related aspects of the research: Section II. Recommended Direct-Strike Median and Extreme Parameters; Section III. Time-Domain Waveforms for First Strokes, Subsequent Strokes, and Continuing Currents; Section IV. Damage to Metal Surfaces by Lightning Currents; and Section V. Measurement of the Characteristics of Positive Lightning. Results of the literature search used to derive the material in Section II and Section IV are found in the Appendices: Appendix 1. Return Stroke Current, Appendix 2. Continuing Current, Appendix 3. Positive Lightning, and Appendix 4. Lightning Damage to Metal Surfaces.

  6. The effects of lightning and high altitude electromagnetic pulse on power distribution lines

    SciTech Connect (OSTI)

    Uman, M.A.; Rubinstein, M.; Yacoub, Z. [Florida Univ., Gainesville, FL (United States)

    1995-01-01

    We simultaneously recorded the voltages induced by lightning on both ends of an unenergized 448-meter long unenergized electric power line and the lightning vertical electric and horizontal magnetic fields at ground level near the line. The lightning data studied and presented here were due both to cloud lightning and to very close (about 20 m from the line) artificially initiated lightning. For cloud sources, a frequency-domain computer program called EMPLIN was used to calculate induced line voltages as a function of source elevation, angle of incidence, and wave polarization of the radiated cloud discharge pulses in order to compare with the measurements. For very-close lightning, the measured line voltages could be grouped into two categories, those in which multiple, similarly shaped, evenly spaced pulses were observed, which we call oscillatory, and those dominated by a principal pulse with subsidiary oscillations of much smaller amplitude, which we call impulsive. The amplitude of the induced voltage ranged from tens of kilovolts for oscillatory voltages to hundreds of kilovolts for impulsive voltages. A new technique is derived for the calculation of the electromagnetic fields from nearby lightning to ground above an imperfectly conducting ground. This technique was used in conjunction with an existing time domain coupling theory and lightning return stroke model to calculate voltages at either end of the line. The results show fair agreement with the measured oscillatory voltage waveforms if corona is ignored and improved results when corona effects are modeled. The modeling of the impulsive voltage, for which local flashover probably successful. In an attempt to understand better the sources of the line voltages for very close lightning, measurements of the horizontal and vertical electric fields 30 m from triggered lightning were obtained.

  7. Little Black Holes:Dark Matter And Ball Lightning

    E-Print Network [OSTI]

    Mario Rabinowitz

    2002-12-11

    Small,quiescent black holes can be considered as candidates for the missing dark matter of the universe,and as the core energy source of ball lightning.By means of gravitational tunneling,directed radiation is emitted from black holes in a process much attenuated from that of Hawking radiation,P SH, which has proven elusive to detect.Gravitational tunneling emission is similar to electric field emission of electronsfrom a metal in that a second body is involved which lowers the barrier and gives the barrier a finite rather than infinite width.Hawking deals with a single isolated black hole.

  8. CEBAF Center Roof Work to Impact Parking Lot, Loading Dock Area...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Roof Work to Impact Parking Lot, Loading Dock Area Beginning on July 20, 2015 and continuing for about two weeks, a subcontractor will be working on the CEBAF Center F Wing roof....

  9. Optimal location of cross-docking centers for a distribution network in Argentina

    E-Print Network [OSTI]

    Dobrusky, Fernando G. (Fernando Gabriel), 1971-

    2003-01-01

    The objective of this thesis is to define an optimal distribution network for Argentina using Cross Docking Centers. The products to be delivered are in a Central Warehouse in Buenos Aires, the main city and port of ...

  10. GRAMM-X public web server for protein-protein docking

    E-Print Network [OSTI]

    Tovchigrechko, Andrey; Vakser, Ilya A.

    2006-07-01

    Protein docking software GRAMM-X and its web interface (http://vakser.bioinformatics.ku.edu/resources/gramm/grammx) extend the original GRAMM Fast Fourier Transformation methodology by employing smoothed potentials, refinement stage, and knowledge...

  11. Connection stiffness and dynamical docking process of flux pinned spacecraft modules

    SciTech Connect (OSTI)

    Lu, Yong; Zhang, Mingliang, E-mail: niudun12@126.com; Gao, Dong [School of Mechatronics Engineering, Harbin Institute of Technology, Harbin, Heilongjiang 150001 (China)

    2014-02-14

    This paper describes a novel kind of potential flux pinned docking system that consists of guidance navigation and control system, the traditional extrusion type propulsion system, and a flux pinned docking interface. Because of characteristics of passive stability of flux pinning, the docking control strategy of flux pinned docking system only needs a series of sequential control rather than necessary active feedback control, as well as avoidance of hazardous collision accident. The flux pinned force between YBaCuO (YBCO) high temperature superconductor bulk and permanent magnet is able to be given vent based on the identical current loop model and improved image dipole model, which can be validated experimentally. Thus, the connection stiffness between two flux pinned spacecraft modules can be calculated based on Hooke's law. This connection stiffness matrix at the equilibrium position has the positive definite performance, which can validate the passively stable connection of two flux pinned spacecraft modules theoretically. Furthermore, the relative orbital dynamical equation of two flux pinned spacecraft modules can be established based on Clohessy-Wiltshire's equations and improved image dipole model. The dynamical docking process between two flux pinned spacecraft modules can be obtained by way of numerical simulation, which suggests the feasibility of flux pinned docking system.

  12. Radar-Derived Forecasts of Cloud-to-Ground Lightning Over Houston, Texas 

    E-Print Network [OSTI]

    Mosier, Richard Matthew

    2011-02-22

    -derived Products....26 1.6 Thesis Objectives and Hypothesis...........................................................................27 2. DATA AND METHODOLOGY..................................................................................29 2.1 Radar............................................................................................42 2.4.4 Storm Cell Position Forecast............................................................................44 2.5 Lightning Correlation..............................................................................................45 2.6 CG...

  13. Long recovery VLF perturbations associated with lightning M. M. Salut,1

    E-Print Network [OSTI]

    .1029/2012JA017567. 1. Introduction [2] The intense electromagnetic pulse (EMP) radiated from powerful cloud with direct coupling of the lightning electromagnetic energy to the lower ionosphere. Also, it has been shown

  14. Cloud-to-ground lightning-precipitation relationships in the South Central United States 

    E-Print Network [OSTI]

    Sheridan, Scott Christopher

    1995-01-01

    Despite the general coexistence of cloud-to-ground (CG) lightning and precipitation, there have been few studies on the nature of the relationship between these two phenomena. This study endeavors to explore this relationship, ...

  15. The distribution of thunderstorm and lightning parameters over the Eastern Hemisphere for 1972 

    E-Print Network [OSTI]

    Freeman, William Burns

    1974-01-01

    - cially over the oceans, North Africa, Arabia, the Mediterranean Sea, and Southeast China. These were areas of sparse data in the W. M. O. compilation but were covered in this investigation. The tentative, first estimate of the distribution of lightning... discharge on a yearly basis were located over South Africa, the Mediterranean Sea, Arabia, Southeast China, Southeast Asia, and Australia. The occurrence of lightning discharge was shown to follow Sun northward from January to the maximum poleward thrust...

  16. On electromagnetic models of ball lightning with topological structure

    E-Print Network [OSTI]

    Donoso, J M; Trueba, J L

    2003-01-01

    It has been long admitted that a consequence of the virial theorem is that there can be no equilibrium configurations of a system of charges in electromagnetic interaction in the absence of external forces. However, recent results have shown that the virial theorem can not preclude the existence of certain nontrivial equilibrium configurations. Although some of these new results are based on an effective microscopic field theory, they are important for a theory of ball lightning that has been developed by the authors of the present work. Other theoretical results relative to magnetic force-free fields with field aligned currents and self-organized filamentary structures are also found to be relevant for this model.

  17. Crusader Automated Docking System: Technology support for the Crusader Resupply Team. Interim report, Ammunition Logistics Program

    SciTech Connect (OSTI)

    Kring, C.T.; Varma, V.K.; Jatko, W.B.

    1995-11-01

    The US Army and Team Crusader (United Defense, Lockheed Martin Armament Systems, etc.) are developing the next generation howitzer, the Crusader. The development program includes an advanced, self-propelled liquid propellant howitzer and a companion resupply vehicle. The resupply vehicle is intended to rendezvous with the howitzer near the battlefront and replenish ammunition, fuel, and other material. The Army has recommended that Crusader incorporate new and innovative technologies to improve performance and safety. One conceptual design proposes a robotic resupply boom on the resupply vehicle to upload supplies to the howitzer. The resupply boom would normally be retracted inside the resupply vehicle during transit. When the two vehicles are within range of the resupply boom, the boom would be extended to a receiving port on the howitzer. In order to reduce exposure to small arms fire or nuclear, biological, and chemical hazards, the crew would remain inside the resupply vehicle during the resupply operation. The process of extending the boom and linking with the receiving port is called docking. A boom operator would be designated to maneuver the boom into contact with the receiving port using a mechanical joystick. The docking operation depends greatly upon the skill of the boom operator to manipulate the boom into docking position. Computer simulations at the National Aeronautics and Space Administration have shown that computer-assisted or autonomous docking can improve the ability of the operator to dock safely and quickly. This document describes the present status of the Crusader Autonomous Docking System (CADS) implemented at Oak Ridge National laboratory (ORNL). The purpose of the CADS project is to determine the feasibility and performance limitations of vision systems to satisfy the autonomous docking requirements for Crusader and conduct a demonstration under controlled conditions.

  18. Thunderstorm characteristics of cloud-to-ground lightning at the Kennedy Space Center, Florida: a study of lightning initiation signatures as indicated by Doppler radar 

    E-Print Network [OSTI]

    Gremillion, Michael Shane

    1998-01-01

    A sum-nary of lightning characteristics was developed for the NASA Kennedy Space Center (KSC). From-n a 1989-1996 data set, the spatial patterns, temporal patterns, and first stroke mean peak current were analyzed. Forty five thunderstorms were...

  19. Lightning -"When thunder roars..." Nearly 400 people in the United States are struck by lightning each year. On average, 60 people will

    E-Print Network [OSTI]

    Meyers, Steven D.

    may be injured if lightning strikes your car, you are much safer inside a vehicle than outside equipment, do so well before the storm arrives. Avoid contact with plumbing. Do not wash your hands, do not take a shower, do not wash dishes, and do not do laundry. Stay away from windows and doors, and stay

  20. In-fight measurements of energetic radiation from lightning and thunderclouds

    E-Print Network [OSTI]

    Kochkin, P O; de Boer, A; Bardet, M; Boissin, J -F

    2015-01-01

    In the certification procedure aircraft builders carry out so-called icing tests flights, where the zero degree Celsius altitude is deliberately sought and crossed in or under thunderstorms. Airbus also used these flights to test ILDAS, a system aimed to determine lightning severity and attachment points during flight from high speed data on the electric and magnetic field at the aircraft surface. We used this unique opportunity to enhance the ILDAS systems with two x-ray detectors coupled to high speed data recorders in an attempt to determine the x-rays produced by lightning in-situ, with synchronous determination of the lightning current distribution and electric field at the aircraft. Such data are of interest in a study of lightning physics. In addition, the data may provide clues to the x-ray dose for personnel and equipment during flights. The icing campaign ran in April 2014; in six flights we collected data of 61 lightning strikes on an Airbus test aircraft. In this communication we briefly describe ...

  1. Radar Nowcasting of Total Lightning over the Kennedy Space Center GREGORY N. SEROKA,* RICHARD E. ORVILLE, AND COURTNEY SCHUMACHER

    E-Print Network [OSTI]

    Radar Nowcasting of Total Lightning over the Kennedy Space Center GREGORY N. SEROKA,* RICHARD E-derived predictors of total lightning over the Kennedy Space Center (KSC). Four years (2006­ 09) of summer (June houses the Kennedy Space Center (KSC) and its critical daily operations. While examining storms over

  2. 532 IEEE TRANSACTIONS ON ELECTROMAGNETIC COMPATIBILITY, VOL. 51, NO. 3, AUGUST 2009 Lightning Electromagnetic Field Coupling to

    E-Print Network [OSTI]

    Florida, University of

    of both the incident lightning electromagnetic pulse (LEMP) and the effects of coupling of this field- mental validation using: 1) reduced-scale setups with LEMP and nuclear electromagnetic pulse (NEMP532 IEEE TRANSACTIONS ON ELECTROMAGNETIC COMPATIBILITY, VOL. 51, NO. 3, AUGUST 2009 Lightning

  3. Protection characteristics of a Faraday cage compromised by lightning burnthrough.

    SciTech Connect (OSTI)

    Warne, Larry Kevin; Bystrom, Edward; Jorgenson, Roy Eberhardt; Montoya, Sandra L.; Merewether, Kimball O.; Coats, Rebecca Sue; Martinez, Leonard E.; Jojola, John M.

    2012-01-01

    A lightning flash consists of multiple, high-amplitude but short duration return strokes. Between the return strokes is a lower amplitude, continuing current which flows for longer duration. If the walls of a Faraday cage are made of thin enough metal, the continuing current can melt a hole through the metal in a process called burnthrough. A subsequent return stroke can couple energy through this newly-formed hole. This LDRD is a study of the protection provided by a Faraday cage when it has been compromised by burnthrough. We initially repeated some previous experiments and expanded on them in terms of scope and diagnostics to form a knowledge baseline of the coupling phenomena. We then used a combination of experiment, analysis and numerical modeling to study four coupling mechanisms: indirect electric field coupling, indirect magnetic field coupling, conduction through plasma and breakdown through the hole. We discovered voltages higher than those encountered in the previous set of experiments (on the order of several hundreds of volts).

  4. Exciton matter sustained by colossal dispersive interactions due to enhanced polarizability: Possible clue to ball lightning

    E-Print Network [OSTI]

    Georgiev, M; Georgiev, Mladen; Singh, Jai

    2005-01-01

    Recently Gilman has pointed out that the material state of a ball lightning is both highly cohesive and flexible. He makes a specific proposal for a cohesive state arising from (colossal) Van-der-Waals attraction between highly polarizable Rydberg atoms produced under a linear lightning. We accept his general suggestions but propose that the colossal Van-der-Waals coupling may also arise from the enhanced polarizability of surrogate molecular clusters, due to the polaron gap narrowing effect. We consider a few illuminating cases and present calculations for the ammonia molecule. Although being unable to identify the exact nature of the surrogate molecules at least for the time-being, we suggest a general scenario of photoexcited vibronic excitons forming a supersaturated surrogate gas phase in which a ball arises as a result of condensation. The orange color of the luminous ball is due to radiative exciton deexcitation and suggests that there may be a unique surrogate material for ball lightning.

  5. Measuring the Allocation of Control in a 6 Degree-of-Freedom Docking Experiment

    E-Print Network [OSTI]

    Milgram, Paul

    and Paul Milgram Ergonomics in Teleoperation and Control (ETC) Lab Department of Mechanical and Industrial Coordination, interaction techniques, allocation of control, virtual docking task, the M-metric, evaluation this question can be addressed, however, it is essential first to develop a metric for evaluating allocation

  6. Evaluation of an approximate method for incorporating floating docks in harbor wave prediction models 

    E-Print Network [OSTI]

    Tang, Zhaoxiang

    2005-11-01

    to Douglas harbor in Alaska. The result shows that docks in the harbor distort the wave field considerably and create a reflective pattern that can affect navigation safety in some areas. Also plots are developed for the transmission coefficients for waves...

  7. A Cooperative Approach to Support Software Deployment Using the Software Dock

    E-Print Network [OSTI]

    Wolf, Alexander L.

    174 A Cooperative Approach to Support Software Deployment Using the Software Dock Richard S. Hall, Dennis Heimbigner, Alexander L. Wolf Software Engineering Research Laboratory University of Colorado Boulder, Colorado 80309-0430 USA [rickhall,dennis,alw]@cs.colorado.edu ABSTRACT Software deployment

  8. A Cooperative Approach to Support Software Deployment Using the Software Dock

    E-Print Network [OSTI]

    van der Hoek, André

    A Cooperative Approach to Support Software Deployment Using the Software Dock Richard S. Hall, Dennis Heimbigner, Alexander L. Wolf Software Engineering Research Laboratory University of Colorado Boulder, Colorado 80309-0430 USA [rickhall,dennis,alw]@cs.colorado.edu ABSTRACT Software deployment

  9. A Cooperative Approach to Support Software Deployment Using the Software Dock

    E-Print Network [OSTI]

    Wolf, Alexander L.

    A Cooperative Approach to Support Software Deployment Using the Software Dock Technical Report CU Software deployment is an evolving collection of interre- lated processes such as release, install, adapt, such as the Internet, is affecting how software deployment is being performed. To take full advantage

  10. Rare measurements of a sprite with halo event driven by a negative lightning discharge over Argentina

    E-Print Network [OSTI]

    Thomas, Jeremy N.

    Argentina M. J. Taylor,1 M. A. Bailey,1 P. D. Pautet,1 S. A. Cummer,2 N. Jaugey,2 J. N. Thomas,3,4 N. N measurements were made of a mesoscale thunderstorm observed on February 22­23, 2006 over northern Argentina of a sprite with halo event driven by a negative lightning discharge over Argentina, Geophys. Res. Lett., 35

  11. The QBO's influence on lightning production and deep convection in the tropics 

    E-Print Network [OSTI]

    Hernandez, Celina Anne

    2009-05-15

    . THE QBO?S INFLUENCE ON LIGHTING PRODUCTION IN THE TROPICS ................................................................................................................ 10 3.1 Africa... lightning flash densities observed over land (Fig.8). Therefore, this analysis focuses on four land regions: Africa, India, Maritime continent/Australia, and South America. 3.1 Africa West minus east flash density anomalies for DJF (Fig. 9a) show...

  12. Cloud-to-Ground Lightning Downwind of the 2002 Hayman Forest Fire in Colorado

    E-Print Network [OSTI]

    Rutledge, Steven

    to climatology, and showed environmental differences that are associated with enhanced +CG lightning. Thus, our findings provide only mixed support for the idea that smoke aerosols impact CG polarity. 2 #12 with smoke aerosol advected northward from fires that burned in southern Mexico during this time. Furthermore

  13. Measurements and implications of the relationship between lightning and terrestrial gamma ray flashes

    E-Print Network [OSTI]

    Cummer, Steven A.

    the discovery of short bursts of gamma rays originating from Earth, called terrestrial gamma ray flashes (TGFsMeasurements and implications of the relationship between lightning and terrestrial gamma ray associated with 26 terrestrial gamma ray flashes (TGFs) recorded by the RHESSI satellite over the Caribbean

  14. Polarity and energetics of inner core lightning in three intense North Atlantic hurricanes

    E-Print Network [OSTI]

    Cummer, Steven A.

    Atlantic hurricanes Jeremy N. Thomas,1,2,3 Natalia N. Solorzano,1,3 Steven A. Cummer,4 and Robert H and energetics of lightning within 100 km of the centers (inner core regions) of North Atlantic hurricanes Emily important for hurricane intensity change forecasting. Additionally, we find that the majority of inner core

  15. The use of Doppler radar to predict cloud-to-ground lightning 

    E-Print Network [OSTI]

    Aclin, Keith Andrew

    1995-01-01

    If the National Weather Service could forecast the occurrences of cloud-toground lightning strikes, it would benefit the authorities that are served by the WSR-88Ds. In this thesis I will examine the WSR-88D level 11 archive ...

  16. News & Blogs U.S. Lightning Strikes May Increase 50% Due to Global Warming

    E-Print Network [OSTI]

    Romps, David M.

    News & Blogs U.S. Lightning Strikes May Increase 50% Due to Global Warming By: Dr. Jeff Masters (/blog/JeffMasters/show.html) , 4:04 PM GMT on November 14, 2014 A warmer world will have much more, (http://www.wunderground.com/blog/JeffMasters/comment.html?entrynum=2320) the Whitewater- Baldy Complex

  17. Measurement of lightning-induced currents in an experimental coaxial buried cable

    E-Print Network [OSTI]

    Florida, University of

    . Cablecross-section. Cable Shield \\ 1-4 ,,,' Polyethylene Tinned Comer Jacket 1 7 mm diameter EPR lnsulatio of a shielded buried cable, both in the cable shield and in the inner conductor. The horizontal magnetic field in the cable shield and in the inner conductor. Simultaneously, the horizontal magnetic field and the lightning

  18. Impact of lightning on the lower ionosphere of Saturn and possible generation of halos and sprites

    E-Print Network [OSTI]

    Ebert, Ute

    -dimensional model of the electric field and electron density is used to estimate the changes of the local electron , C. Price a a Department of Geophysical, Atmospheric and Planetary Sciences, Tel-Aviv University and find that the conservative estimation of lightning charge moment change 104 to 105 C km could lead

  19. Development of a guidance, navigation and control architecture and validation process enabling autonomous docking to a tumbling satellite

    E-Print Network [OSTI]

    Nolet, Simon, 1975-

    2007-01-01

    The capability to routinely perform autonomous docking is a key enabling technology for future space exploration, as well as assembly and servicing missions for spacecraft and commercial satellites. Particularly, in more ...

  20. Low-level convergence and its role in convective intensity and frequency over the Houston lightning and rainfall anomaly 

    E-Print Network [OSTI]

    McNear, Veronica Ann

    2007-09-17

    An increase in the amount of lightning and rainfall over the Houston area, compared to the surrounding rural areas, has been well documented in previous studies. The placement of a Shared Mobile Atmospheric Research and ...

  1. Four photos were stacked together to make this incredible image of lightning over Marysville, Kan., on Sept. 9, 2014.

    E-Print Network [OSTI]

    Romps, David M.

    for more than half of U.S. wildfires, putting pressure on human infrastructure as well as natural digging deeper into the data. To start, Romps made some simple maps: One showed lightning strike data over

  2. A monthly climatological study of cloud-to-ground lightning characteristics throughout the contiguous United States: 1989-1994 

    E-Print Network [OSTI]

    Silver, Alan Corey

    1995-01-01

    The National Lightning Detection Network (NLDN) has been in operation since 1989. The NLDN is currently composed of a mixture of magnetic direction finders (DFs) and time-of-arrival (TOA) sensors which locate cloud-to-ground ...

  3. A study of lightning activity over the warm pool western Pacific Ocean (TOGA-COARE region) for 1993 

    E-Print Network [OSTI]

    Rios, Luis Alberto

    1995-01-01

    The warm pool western Pacific Ocean is an area of the equatorial tropics characterized by strong and frequent convection, and vigorous lightning activity. However, it has been noted by various researchers that the vast oceanic expanses experience...

  4. Exploration of the meteorological characteristics leading to the rapid cessation of cloud-to-ground lightning in winter cyclones along the East Coast of the United States 

    E-Print Network [OSTI]

    Demetriades, Nicholas William Snow

    1999-01-01

    . This study shed light on some of the aspens of winter CG lightning during intense East Coast cyclones. However, many more studies are needed within this field....

  5. New Revelation of Lightning Ball Observation and Proposal for a Nuclear Reactor Fusion Experiment

    E-Print Network [OSTI]

    Domokos Tar

    2009-10-12

    In this paper, the author brings further details regarding his Lightning Ball observation that were not mentioned in the first one (Ref.1-2). Additionally, he goes more into detail as the three forces that are necessary to allow the residual crescent form the hydrodynamic vortex ring to shrink into a sphere.Further topics are the similarities and analogies between the Lightning Ball formation's theory and the presently undertaken Tokamak-Stellarator-Spheromak fusion reactor experiments. A new theory and its experimental realisation are proposed as to make the shrinking of the hot plasma of reactors into a ball possible by means of the so called long range electromagnetic forces. In this way,the fusion ignition temperature could possibly atteined.

  6. New Revelation of Lightning Ball Observation and Proposal for a Nuclear Reactor Fusion Experiment

    E-Print Network [OSTI]

    Tar, Domokos

    2009-01-01

    In this paper, the author brings further details regarding his Lightning Ball observation that were not mentioned in the first one (Ref.1-2). Additionally, he goes more into detail as the three forces that are necessary to allow the residual crescent form the hydrodynamic vortex ring to shrink into a sphere.Further topics are the similarities and analogies between the Lightning Ball formation's theory and the presently undertaken Tokamak-Stellarator-Spheromak fusion reactor experiments. A new theory and its experimental realisation are proposed as to make the shrinking of the hot plasma of reactors into a ball possible by means of the so called long range electromagnetic forces. In this way,the fusion ignition temperature could possibly atteined.

  7. A Ball Lightning Model as a Possible Explanation of Recently Reported Cavity Lights

    SciTech Connect (OSTI)

    Fryberger, David; /SLAC

    2009-08-04

    The salient features of cavity lights, in particular, mobile luminous objects (MLO's), as have been experimentally observed in superconducting accelerator cavities, are summarized. A model based upon standard electromagnetic interactions between a small particle and the 1.5 GHz cavity excitation field is described. This model can explain some features of these data, in particular, the existence of particle orbits without wall contact. While this result is an important success for the model, it is detailed why the model as it stands is incomplete. It is argued that no avenues for a suitable extension of the model through established physics appear evident, which motivates an investigation of a model based upon a more exotic object, ball lightning. As discussed, further motivation derives from the fact that there are significant similarities in many of the qualitative features of ball lightning and MLO's, even though they appear in quite different circumstances and differ in scale by orders of magnitude. The ball lightning model, which incorporates electromagnetic charges and currents, is based on a symmetrized set of Maxwell's equations in which the electromagnetic sources and fields are characterized by a process called dyality rotation. It is shown that a consistent mathematical description of dyality rotation as a physical process can be achieved by adding suitable (phenomenological) current terms to supplement the usual current terms in the symmetrized Maxwell's equations. These currents, which enable the conservation of electric and magnetic charge, are called vacuum currents. It is shown that the proposed ball lightning model offers a good qualitative explanation of the perplexing aspects of the MLO data. Avenues for further study are indicated.

  8. Comments in support of the dipole model of ball lightning (BL)

    E-Print Network [OSTI]

    Soshnikov, V N

    2010-01-01

    The evaluations are presented justifying previously proposed dipole model of ball lightning (BL). The movement and energy supporting the dipole BL are due to the atmospheric electric field. Stability of the BL is due to the fundamental role of energy loss by radiation. An example is presented of calculating the stability of variant of BL. There is also a possible connection of stability BL with a statistical distributions of the atmospheric electric field in time and space.

  9. Measurement and modeling of transfer functions for lightning coupling into the Sago mine.

    SciTech Connect (OSTI)

    Morris, Marvin E.; Higgins, Matthew B.

    2007-04-01

    This report documents measurements and analytical modeling of electromagnetic transfer functions to quantify the ability of cloud-to-ground lightning strokes (including horizontal arc-channel components) to couple electromagnetic energy into the Sago mine located near Buckhannon, WV. Two coupling mechanisms were measured: direct and indirect drive. These transfer functions are then used to predict electric fields within the mine and induced voltages on conductors that were left abandoned in the sealed area of the Sago mine.

  10. Formation mechanism of the atmospheric ball lightning using the triple Beltrami equation

    E-Print Network [OSTI]

    Taveira, A M A; Taveira, Ana Marcia Alves; Sakanaka, Paulo Hiroshi

    2004-01-01

    Ball lightning, also known as fire ball, is a luminous globe which occurs in the course of a thunderstorm. Taking as model of fire ball, the two fluid plasma consisting of electrons and one species ions, and considering that the plasma flow is a finite quantity, we can derive the equation of relaxed energy state, maintaining the helicity constant, in the form of triple Beltramiequations for the magnetic field: s curl x curl x curl x B + p curl x curl x B +q curl xB +r B = 0. Where B is the magnetic field s, p, q, and r are constants to be determined through boundary conditions. This equation is coupled with an equation which describes the hydrodynamic vortex. The problem of the formation of an isolated luminous mass in the sky and the moderate persistence of the resulting form combined with observations of ball lightning describing hollow globes, surface coronas and rapid rotation led to theories depicting ball lightning as a vortex. We explore the solutions of this equation using the method of Chandrasekhar-...

  11. Physical conditions of the ball lightning ejection caused by interaction of electrical discharge with metal and polymer

    E-Print Network [OSTI]

    Emelin, S E; Skvortsov, G E; Bychkov, V L

    2001-01-01

    Physical conditions of formation of the ball lightning with high energy density considered as condensate of heavily excited atoms have been studied. In our approach the ball lightning formation processes is considered as a form of energy-structure self-organi-zation on the base of metastable substance and interchange. We propose different variants of the experimental method to create autonomous formation on the base of the closed capillary discharge allowing to obtain the objects with the life time up to 2s which burn through the foil, explode and can penetrate through the polymer wall without destroying it.

  12. Advance the DNA computing 

    E-Print Network [OSTI]

    Qiu, Zhiquan Frank

    2004-09-30

    It has been previously shown that DNA computing can solve those problems currently intractable on even the fastest electronic computers. The algorithm design for DNA computing, however, is not straightforward. A strong background in both the DNA...

  13. Voltages across assembly joints due to direct-strike lightning currents

    SciTech Connect (OSTI)

    Dinallo, M.S. [Quatro Corp., Albuquerque, NM (United States); Fisher, R.J. [Sandia National Labs., Albuquerque, NM (United States)

    1994-08-01

    An extensive set of direct-strike lightning tests has been carried out on a set of facsimile assembly joints of the kinds employed in the design of nuclear weapon cases. Taken as a whole, the test hardware included all the conceptual design elements that are embodied, either singly or in combination, in any specific assembly joint incorporated into any stockpiled weapon. During the present testing, the effects of all key design parameters on the voltages developed across the interior of the joints were investigated under a range of lightning stroke current parameter values. Design parameter variations included the types and number of joint fasteners, mechanical preload, surface finish tolerance and coatings, and the material from which the joint assembly was fabricated. Variations of the simulated lightning stroke current included amplitude (30-, 100-, and 200-kA peak), rise time, and decay time. The maximum voltage observed on any of the test joints that incorporated proper metal-to-metal surface contact was 65 V. Typical response values were more on the order of 20 V. In order to assess the effect of the presence of a dielectric coating (either intentional or as a result of corrosion) between the mating surfaces of a joint, a special configuration was tested in which the mating parts of the test assembly were coated with a 1-mil-thick dielectric anodizing layer. First strokes to these test assemblies resulted in very narrow voltage spikes of amplitudes up to 900 V. The durations of these spikes were less than 0.1 {mu}s. However, beyond these initial spikes, the voltages typically had amplitudes of up to 400 V for durations of 3 to 5 {mu}s.

  14. A unified radiative magnetohydrodynamics code for lightning-like discharge simulations

    SciTech Connect (OSTI)

    Chen, Qiang, E-mail: cq0405@126.com; Chen, Bin, E-mail: emcchen@163.com; Xiong, Run; Cai, Zhaoyang [National Key Laboratory of Electromagnetic Environment and Electro-optical Engineering, PLA University of Science and Technology, Nanjing 210007 (China)] [National Key Laboratory of Electromagnetic Environment and Electro-optical Engineering, PLA University of Science and Technology, Nanjing 210007 (China); Chen, P. F., E-mail: chenpf@nju.edu.cn [School of Astronomy and Space Science, Nanjing University, Nanjing 210093 (China)

    2014-03-15

    A two-dimensional Eulerian finite difference code is developed for solving the non-ideal magnetohydrodynamic (MHD) equations including the effects of self-consistent magnetic field, thermal conduction, resistivity, gravity, and radiation transfer, which when combined with specified pulse current models and plasma equations of state, can be used as a unified lightning return stroke solver. The differential equations are written in the covariant form in the cylindrical geometry and kept in the conservative form which enables some high-accuracy shock capturing schemes to be equipped in the lightning channel configuration naturally. In this code, the 5-order weighted essentially non-oscillatory scheme combined with Lax-Friedrichs flux splitting method is introduced for computing the convection terms of the MHD equations. The 3-order total variation diminishing Runge-Kutta integral operator is also equipped to keep the time-space accuracy of consistency. The numerical algorithms for non-ideal terms, e.g., artificial viscosity, resistivity, and thermal conduction, are introduced in the code via operator splitting method. This code assumes the radiation is in local thermodynamic equilibrium with plasma components and the flux limited diffusion algorithm with grey opacities is implemented for computing the radiation transfer. The transport coefficients and equation of state in this code are obtained from detailed particle population distribution calculation, which makes the numerical model is self-consistent. This code is systematically validated via the Sedov blast solutions and then used for lightning return stroke simulations with the peak current being 20?kA, 30?kA, and 40?kA, respectively. The results show that this numerical model consistent with observations and previous numerical results. The population distribution evolution and energy conservation problems are also discussed.

  15. Quickstart guide to proteinprotein docking using Rosetta 3.1 This is one of a series of tutorials designed to get you started with protein docking using Rosetta 3.1. It was

    E-Print Network [OSTI]

    Meiler, Jens

    from 1KXQ.pdb (q.v.), a complex of amylase with a camel antibody. For this exercise, the file domain was manually moved 20 Angstroms away from the amylase. Docking partner 1 (chain A) is the larger::randomize1, which allows full rotation of the amylase, while keeping the antibody domain oriented

  16. Synthesis of DNA

    DOE Patents [OSTI]

    Mariella, Jr., Raymond P. (Danville, CA)

    2008-11-18

    A method of synthesizing a desired double-stranded DNA of a predetermined length and of a predetermined sequence. Preselected sequence segments that will complete the desired double-stranded DNA are determined. Preselected segment sequences of DNA that will be used to complete the desired double-stranded DNA are provided. The preselected segment sequences of DNA are assembled to produce the desired double-stranded DNA.

  17. A census of precipitation features in the tropics using TRMM: radar, ice scattering, and lightning observations 

    E-Print Network [OSTI]

    Nesbitt, Stephen William

    1999-01-01

    . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73 39 LIS lightning flashes within 8 km of TPFs . . . 76 40 Flashes per km' TPF area vs. volumetric rainfall in TPFs in Africa, S. America, E. Pacific, and W. Pacific . . . . . . . . . . . . . . . . . . . . . . . . 77 41 Map of all TPFs over... Africa 42 Map of all TPFs with a pixel of PCT & 250 K over Africa . . . . . 43 Map of all TPFs over S. America. . 87 . . . . 88 89 44 Map of all TPFs with a pixel of PCT & 250 K over S. America . . . . . . . . 90 45 Map of all TPFs over the E...

  18. DNA Engine Thermal Cycler

    E-Print Network [OSTI]

    Raizada, Manish N.

    ® Peltier Thermal Cycler PTC-0200 DNA Engine Cycler Operations Manual Version 4.0 #12;ii Tech Support: 1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .vi The DNA Engine® Peltier Thermal Cycler Introduction

  19. Structural basis for inhibition of DNA replication by aphidicolin

    SciTech Connect (OSTI)

    Baranovskiy, A. G.; Babayeva, N. D.; Suwa, Y.; Gu, J.; Pavlov, Y. I.; Tahirov, T. H.

    2014-11-27

    Natural tetracyclic diterpenoid aphidicolin is a potent and specific inhibitor of B-family DNA polymerases, haltering replication and possessing a strong antimitotic activity in human cancer cell lines. Clinical trials revealed limitations of aphidicolin as an antitumor drug because of its low solubility and fast clearance from human plasma. The absence of structural information hampered the improvement of aphidicolin-like inhibitors: more than 50 modifications have been generated so far, but all have lost the inhibitory and antitumor properties. Here we report the crystal structure of the catalytic core of human DNA polymerase ? (Pol ?) in the ternary complex with an RNA-primed DNA template and aphidicolin. The inhibitor blocks binding of dCTP by docking at the Pol ? active site and by rotating the template guanine. The structure provides a plausible mechanism for the selectivity of aphidicolin incorporation opposite template guanine and explains why previous modifications of aphidicolin failed to improve its affinity for Pol ?. With new structural information, aphidicolin becomes an attractive lead compound for the design of novel derivatives with enhanced inhibitory properties for B-family DNA polymerases.

  20. Structural basis for inhibition of DNA replication by aphidicolin

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Baranovskiy, A. G.; Babayeva, N. D.; Suwa, Y.; Gu, J.; Pavlov, Y. I.; Tahirov, T. H.

    2014-11-27

    Natural tetracyclic diterpenoid aphidicolin is a potent and specific inhibitor of B-family DNA polymerases, haltering replication and possessing a strong antimitotic activity in human cancer cell lines. Clinical trials revealed limitations of aphidicolin as an antitumor drug because of its low solubility and fast clearance from human plasma. The absence of structural information hampered the improvement of aphidicolin-like inhibitors: more than 50 modifications have been generated so far, but all have lost the inhibitory and antitumor properties. Here we report the crystal structure of the catalytic core of human DNA polymerase ? (Pol ?) in the ternary complex with anmore »RNA-primed DNA template and aphidicolin. The inhibitor blocks binding of dCTP by docking at the Pol ? active site and by rotating the template guanine. The structure provides a plausible mechanism for the selectivity of aphidicolin incorporation opposite template guanine and explains why previous modifications of aphidicolin failed to improve its affinity for Pol ?. With new structural information, aphidicolin becomes an attractive lead compound for the design of novel derivatives with enhanced inhibitory properties for B-family DNA polymerases.« less

  1. Task-parallel message passing interface implementation of Autodock4 for docking of very large databases of compounds using high-performance super-computers

    SciTech Connect (OSTI)

    Collignon, Barbara C; Schultz, Roland; Smith, Jeremy C; Baudry, Jerome Y

    2011-01-01

    A message passing interface (MPI)-based implementation (Autodock4.lga.MPI) of the grid-based docking program Autodock4 has been developed to allow simultaneous and independent docking of multiple compounds on up to thousands of central processing units (CPUs) using the Lamarkian genetic algorithm. The MPI version reads a single binary file containing precalculated grids that represent the protein-ligand interactions, i.e., van der Waals, electrostatic, and desolvation potentials, and needs only two input parameter files for the entire docking run. In comparison, the serial version of Autodock4 reads ASCII grid files and requires one parameter file per compound. The modifications performed result in significantly reduced input/output activity compared with the serial version. Autodock4.lga.MPI scales up to 8192 CPUs with a maximal overhead of 16.3%, of which two thirds is due to input/output operations and one third originates from MPI operations. The optimal docking strategy, which minimizes docking CPU time without lowering the quality of the database enrichments, comprises the docking of ligands preordered from the most to the least flexible and the assignment of the number of energy evaluations as a function of the number of rotatable bounds. In 24 h, on 8192 high-performance computing CPUs, the present MPI version would allow docking to a rigid protein of about 300K small flexible compounds or 11 million rigid compounds.

  2. Physical mechanism and numerical simulation of the inception of the lightning upward leader

    SciTech Connect (OSTI)

    Li Qingmin [Beijing Key Lab of High Voltage and EMC, School of Electric and Electronic Engineering, North China Electric Power University, Beijing 102206 (China) and State Key Lab of Alternate Electrical Power System with Renewable Energy Sources, North China Electric Power University, Beijing 102206 (China); Lu Xinchang; Shi Wei; Zhang Li; Zou Liang; Lou Jie [Shandong Provincial Key Lab of UHV Technology and Gas Discharge, School of Electrical Engineering, Shandong University, Jinan 250061 (China)

    2012-12-15

    The upward leader is a key physical process of the leader progression model of lightning shielding. The inception mechanism and criterion of the upward leader need further understanding and clarification. Based on leader discharge theory, this paper proposes the critical electric field intensity of the stable upward leader (CEFISUL) and characterizes it by the valve electric field intensity on the conductor surface, E{sub L}, which is the basis of a new inception criterion for the upward leader. Through numerical simulation under various physical conditions, we verified that E{sub L} is mainly related to the conductor radius, and data fitting yields the mathematical expression of E{sub L}. We further establish a computational model for lightning shielding performance of the transmission lines based on the proposed CEFISUL criterion, which reproduces the shielding failure rate of typical UHV transmission lines. The model-based calculation results agree well with the statistical data from on-site operations, which show the effectiveness and validity of the CEFISUL criterion.

  3. The evolution of total lightning and radar reflectivity characteristics of two mesoscale convective systems over Houston, Texas 

    E-Print Network [OSTI]

    Hodapp, Charles Lee

    2009-05-15

    production of MCSs. The stratiform areas quadruple in size and the mean reflectivity values increase substantially by ~ 6 dB. As the stratiform region matures, VHF source density plots show a lightning pathway that slopes rearward and downward from the back...

  4. Charge transfer and in-cloud structure of large-charge-moment positive lightning strokes in a mesoscale convective system

    E-Print Network [OSTI]

    Cummer, Steven A.

    in a mesoscale convective system Gaopeng Lu,1 Steven A. Cummer,1 Jingbo Li,1 Feng Han,1 Richard J. Blakeslee,2 positive cloud-to-ground (+CG) strokes in a mesoscale convective system. Although no high altitude images of large-charge-moment positive lightning strokes in a mesoscale convective system, Geophys. Res. Lett., 36

  5. Cloud-to-ground lightning downwind of the 2002 Hayman forest fire in Timothy J. Lang and Steven A. Rutledge

    E-Print Network [OSTI]

    Rutledge, Steven

    Cloud-to-ground lightning downwind of the 2002 Hayman forest fire in Colorado Timothy J. Lang February 2006. [1] The Hayman forest fire occurred near Denver, burning $138,000 acres during 8 June­2 July forest fire in Colorado, Geophys. Res. Lett., 33, L03804, doi:10.1029/2005GL024608. 1. Introduction [2

  6. Report on an Explosion and Fire involving the Motor Vessel Atlantic Duchess at Queen's Dock, Swansea, Glamorganshire on 2nd February, 1951 

    E-Print Network [OSTI]

    Watts, H. E.

    1951-04-02

    REPORT TO THE RIGHT HONOURABLE THE SECRETARY OF STATE FOR THE HOME DEPARTMENT ON THE CIRCUMSTANCES ATTENDING AN EXPLOSION AND FIRE WHICH OCCURRED ON 2ND FEBRUARY. 1951, AT QUEEN'S DOCK SWANSEA, IN THE COUNTY OF ...

  7. IMPEE PhD Opportunity Project title: LightFoot PhD A PhD Investigation Lightning Protection of HV Overhead Lines with Non-Ideal

    E-Print Network [OSTI]

    Greenaway, Alan

    IMPEE PhD Opportunity Project title: LightFoot PhD ­ A PhD Investigation Lightning Protection of HV.swingler@hw.ac.uk Abstract LightFoot PhD ­ A PhD Investigation Lightning Protection of HV Overhead Lines with Non-Ideal Tower Footing Resistance funded by SSE. The LightFoot PhD project takes a fresh approach at adding

  8. Failure analysis of rutile sleeves in MC3080 lightning arrestor connectors.

    SciTech Connect (OSTI)

    Kilgo, Alice C.; Monroe, Saundra L.; Watson, Chad Samuel; Ernest, Terry L.

    2006-02-01

    The purpose of this SAND Report is to document efforts in the extraction and failure analyses of sleeve-style Lightning Arrestor Connectors (LACs). Several MC3080 and MC3079 LACs were recovered from the field and tested as part of the Enhanced Surveillance Campaign. A portion of these LACs failed retesting. Terry Ernest (01733), the LAC Component Engineer, provided eleven MC3080 LACs for evaluation where four of the LACs failed IR/DCW and one failed FRB requirements. The extraction of rutile sleeves from failed LACs was required to determine the source of failure. Rutile sleeves associated with connector function failures were examined for cracks, debris as well as any other anomalies which could have caused the LAC to not function properly. Sleeves that failed FRB or that experienced high FRB exhibited high symmetry, smooth surface, long-flow amicon, and slightly over-sized inside diameter. LACs that failed DCW or IR requirements had rutile sleeves that exhibited breakdown tracks.

  9. Comments to support the Dipole Dynamical Model (DDM) of Ball Lightning (BL)

    E-Print Network [OSTI]

    V. N. Soshnikov

    2015-08-13

    I present estimates to justify previously proposed by me heuristic Dipole Dynamical Model (DDM) of Ball Lightning (BL). The movement and energy supplying to the dipole BL are due to the atmospheric electric field. Crucial for the detailed analysis of BL is using the new relation of balance of the force of atmospheric electric field (per unit mass of electron cloud) and dipole forces electrons-ions within BL dipole (per unit mass of BL) as the first necessary condition for the existance of BL as an integer. This model is unique because, unlike existing static models, fundamental condition for the existence of Ball Lightning is its forward motion. The virial theorem limiting BL power does not apply to BL which is not closed system like the Sun or Galaxy systems and is strongly dependent part of the infinitely extended in time and space large system. Stability of BL is due to two free parameters with the fundamental role of thermodynamic non-equilibrium, ionization, recombination and translational movement with energy loss by radiation and also excess volumetric positive charge. Stability of BL is not related to the presence of any external shells. Polarization degree of BL plasma is characterized by polarizability factor {\\gamma}. An example is presented of calculating the stability of option BL. There is also a possible connection of stability BL with statistical distributions of the atmospheric electric field in time and space. Destruction of BL can also occur due to arising kinematical instability at its accelerating (or decelerating) movement. Maximal energy density in BL DDM does not exceed the value Espec<(10(8) - 10(9)) J/m(3). Resulting indefinitely long BL lifetime is also discussed. BL has no outer shell and no any inner rigid microstructure elements.

  10. Comments to support the Dipole Dynamical Model (DDM) of Ball Lightning (BL)

    E-Print Network [OSTI]

    V. N. Soshnikov

    2015-10-03

    I present estimates to justify previously proposed by me heuristic Dipole Dynamical Model (DDM) of Ball Lightning (BL). The movement and energy supplying to the dipole BL are due to the atmospheric electric field. Crucial for the detailed analysis of BL is using the new relation of balance of the force of atmospheric electric field (per unit mass of electron cloud) and dipole forces electrons-ions within BL dipole (per unit mass of BL) as the first necessary condition for the existance of BL as an integer. This model is unique because, unlike existing static models, fundamental condition for the existence of Ball Lightning is its forward motion. The virial theorem limiting BL power does not apply to BL which is not closed system like the Sun or Galaxy systems and is strongly dependent part of the infinitely extended in time and space large system. Stability of BL is due to two free parameters with the fundamental role of thermodynamic non-equilibrium, ionization, recombination and translational movement with energy loss by radiation and also excess volumetric positive charge. Stability of BL is not related to the presence of any external shells. Polarization degree of BL plasma is characterized by polarizability factor {\\gamma}. An example is presented of calculating the stability of an option of BL. There is also a possible connection of stability BL with statistical distributions of the atmospheric electric field in time and space. Destruction of BL can also occur due to arising kinematical instability at its accelerating (or decelerating) movement. Maximal energy density in BL DDM does not exceed the value Espec<(10(8) - 10(9)) J/m(3) decreasing with the growing BL radius. Resulting indefinitely long BL lifetime is also discussed. BL has no outer shell and no any inner rigid or elastic microstructure elements.

  11. DNA Topology: Fundamentals

    E-Print Network [OSTI]

    Mirkin, Sergei

    in Genome Functioning . Biological Role of Alternative DNA Structures Figure 1 A hypothetical circular DNA. 1ENCYCLOPEDIA OF LIFE SCIENCES / & 2001 Nature Publishing Group / www.els.net #12;topological

  12. Application of Generalized Quantum Hydrodynamics In the Theory of Quantum Soliton's Evolution, Atom Structure and Lightning Ball

    E-Print Network [OSTI]

    Alexeev, Boris V

    2008-01-01

    Quantum solitons are discovered with the help of generalized quantum hydrodynamics (GQH). The solitons have the character of the stable quantum objects in the self consistent electric field. These effects can be considered as explanation of the existence of lightning balls. The delivered theory demonstrates the great possibilities of the generalized quantum hydrodynamics in investigation of the quantum solitons. The paper can be considered also as comments and prolongation of the materials published in the known author`s monograph (Boris V. Alexeev, Generalized Boltzmann Physical Kinetics. Elsevier. 2004). The theory leads to solitons as typical formations in the generalized quantum hydrodynamics. Key words: Foundations of the theory of transport processes; The theory of solitons; Generalized hydrodynamic equations; Foundations of quantum mechanics; The theory of lightning balls. PACS: 67.55.Fa, 67.55.Hc

  13. DNA Sequencing apparatus

    DOE Patents [OSTI]

    Tabor, Stanley (Cambridge, MA); Richardson, Charles C. (Chestnut Hill, MA)

    1992-01-01

    An automated DNA sequencing apparatus having a reactor for providing at least two series of DNA products formed from a single primer and a DNA strand, each DNA product of a series differing in molecular weight and having a chain terminating agent at one end; separating means for separating the DNA products to form a series bands, the intensity of substantially all nearby bands in a different series being different, band reading means for determining the position an This invention was made with government support including a grant from the U.S. Public Health Service, contract number AI-06045. The U.S. government has certain rights in the invention.

  14. Detection and quantitation of single nucleotide polymorphisms, DNA sequence variations, DNA mutations, DNA damage and DNA mismatches

    DOE Patents [OSTI]

    McCutchen-Maloney, Sandra L. (Pleasanton, CA)

    2002-01-01

    DNA mutation binding proteins alone and as chimeric proteins with nucleases are used with solid supports to detect DNA sequence variations, DNA mutations and single nucleotide polymorphisms. The solid supports may be flow cytometry beads, DNA chips, glass slides or DNA dips sticks. DNA molecules are coupled to solid supports to form DNA-support complexes. Labeled DNA is used with unlabeled DNA mutation binding proteins such at TthMutS to detect DNA sequence variations, DNA mutations and single nucleotide length polymorphisms by binding which gives an increase in signal. Unlabeled DNA is utilized with labeled chimeras to detect DNA sequence variations, DNA mutations and single nucleotide length polymorphisms by nuclease activity of the chimera which gives a decrease in signal.

  15. Introduction: DNA Electrophoresis Fralin Life Science

    E-Print Network [OSTI]

    Hopkins, William A.

    .................................... 12 Student Pre-Lab Activity: What is DNA? DNA extraction from strawberry ..... Teacher guide: DNA extraction from strawberry.................................. 14 Student guide: DNA extraction from strawberry.................................. 16

  16. n-Dimensional Gravity Little Black Holes, Dark Matter, and Ball Lightning

    E-Print Network [OSTI]

    Rabinowitz, M

    2001-01-01

    The gravitational field, and radiation from quantized gravitational atoms and little black holes (LBH) are analyzed in n-space, i.e. in all dimensions from 0 to o to develop insights into possible additional compacted dimensions as predicted by hierarchy and string theory. It is shown that the entropy of LBH is significantly greater in higher dimensional space with potential implications to the initial entropy of the universe. A case is made that LBH are the dark matter of the universe, and can manifest themselves as the core energy source of ball lightning (BL). The LBH incidence rate on earth is related to BL occurrence and has the potential of aiding in the determination of the distribution of LBH and hence dark matter in the universe. Examination of LBH interactions with the atmosphere are found to be in accord with observations of BL. Possibilities are explored as to why Hawking radiation has been undetected in over 25 years. An alternate LBH tunneling radiation model is described.

  17. n-Dimensional Gravity: Little Black Holes, Dark Matter, and Ball Lightning

    E-Print Network [OSTI]

    Mario Rabinowitz

    2003-03-30

    The gravitational field, and radiation from quantized gravitational atoms and little black holes (LBH) are analyzed in n-space, i.e. in all dimensions to develop insights into possible additional compacted dimensions as predicted by hierarchy and string theory. It is shown that the entropy of LBH is significantly greater in higher dimensional space with potential implications to the initial entropy of the universe. A case is made that LBH are the dark matter of the universe, and can manifest themselves as the core energy source of ball lightning (BL). The LBH incidence rate on earth is related to BL occurrence and has the potential of aiding in the determination of the distribution of LBH and hence dark matter in the universe. Examination of LBH interactions with the atmosphere are found to be in accord with observations of BL. Possibilities are explored as to why Hawking radiation has been undetected in over 25 years. An alternate LBH tunneling radiation model is described.

  18. Proposed Southline Transmission Line Project - Volume 2 of 4

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    would contribute to cumulative impacts on wildlife resources include the 27 Sapphire Energy Algae Facility, Lightning Dock Geothermal Power Plant, and Bowie Power Station. 28...

  19. GRED Studies and Drilling of Americulture State 2, Americulture...

    Open Energy Info (EERE)

    GRED Studies and Drilling of Americulture State 2, Americulture Tilapia Farm: Lightning Dock KGRA, Las Animas Valley, New Mexico Jump to: navigation, search OpenEI Reference...

  20. Evidence for Large-Scale Laramide Tectonic Inversion and a Mid...

    Open Energy Info (EERE)

    Laramide Tectonic Inversion and a Mid-Tertiary Caldera Ring Fracture Zone at the Lightning Dock Geothermal System, New Mexico Jump to: navigation, search OpenEI Reference...

  1. Chimeric proteins for detection and quantitation of DNA mutations, DNA sequence variations, DNA damage and DNA mismatches

    DOE Patents [OSTI]

    McCutchen-Maloney, Sandra L. (Pleasanton, CA)

    2002-01-01

    Chimeric proteins having both DNA mutation binding activity and nuclease activity are synthesized by recombinant technology. The proteins are of the general formula A-L-B and B-L-A where A is a peptide having DNA mutation binding activity, L is a linker and B is a peptide having nuclease activity. The chimeric proteins are useful for detection and identification of DNA sequence variations including DNA mutations (including DNA damage and mismatches) by binding to the DNA mutation and cutting the DNA once the DNA mutation is detected.

  2. Becky Hill Green Mountain DNA Conference LT-DNA Analysis

    E-Print Network [OSTI]

    Becky Hill ­ Green Mountain DNA Conference LT-DNA Analysis July 26, 2010 http of the Chief Medical Examiner, NYC Green Mountain DNA Conference Burlington, VT July 26, 2010 Low Template (LT generally aim for 0.5-2 ng 100 pg template 5 pg template #12;Becky Hill ­ Green Mountain DNA Conference LT

  3. In the following questions, please tell us about your fishing activity and experience. "Shore" fishing can include fishing from a beach, bank, jetty, pier, dock, bridge, break-

    E-Print Network [OSTI]

    In the following questions, please tell us about your fishing activity and experience. "Shore" fishing can include fishing from a beach, bank, jetty, pier, dock, bridge, break- water, causeway person to go fishing. A "charterboat" is a boat which a group of people have paid a flat fee for use

  4. Annual Report: 2011-2012 Storm Season Sampling, Non-Dry Dock Stormwater Monitoring for Puget Sound Naval Shipyard, Bremerton, WA

    SciTech Connect (OSTI)

    Brandenberger, Jill M.; Metallo, David; Rupert, Brian; Johnston, Robert K.; Gebhart, Christine

    2013-07-03

    Annual PSNS non-dry dock storm water monitoring results for 2011-2012 storm season. Included are a brief description of the sampling procedures, storm event information, laboratory methods and data collection, a results and discussion section, and the conclusions and recommendations.

  5. Multiplex analysis of DNA

    DOE Patents [OSTI]

    Church, George M. (Boston, MA); Kieffer-Higgins, Stephen (Dorchester, MA)

    1992-01-01

    This invention features vectors and a method for sequencing DNA. The method includes the steps of: a) ligating the DNA into a vector comprising a tag sequence, the tag sequence includes at least 15 bases, wherein the tag sequence will not hybridize to the DNA under stringent hybridization conditions and is unique in the vector, to form a hybrid vector, b) treating the hybrid vector in a plurality of vessels to produce fragments comprising the tag sequence, wherein the fragments differ in length and terminate at a fixed known base or bases, wherein the fixed known base or bases differs in each vessel, c) separating the fragments from each vessel according to their size, d) hybridizing the fragments with an oligonucleotide able to hybridize specifically with the tag sequence, and e) detecting the pattern of hybridization of the tag sequence, wherein the pattern reflects the nucleotide sequence of the DNA.

  6. Shear Unzipping of DNA

    E-Print Network [OSTI]

    Buddhapriya Chakrabarti; David R. Nelson

    2009-04-09

    We study theoretically the mechanical failure of a simple model of double stranded DNA under an applied shear. Starting from a more microscopic Hamiltonian that describes a sheared DNA, we arrive at a nonlinear generalization of a ladder model of shear unzipping proposed earlier by deGennes [deGennes P. G. C. R. Acad. Sci., Ser. IV; Phys., Astrophys. 2001, 1505]. Using this model and a combination of analytical and numerical methods, we study the DNA "unzipping" transition when the shearing force exceeds a critical threshold at zero temperature. We also explore the effects of sequence heterogeneity and finite temperature and discuss possible applications to determine the strength of colloidal nanoparticle assemblies functionalized by DNA.

  7. The kinematic and cloud-to-ground lightning structure of the 9-10, June 1998 Red River Mesoscale Convective System 

    E-Print Network [OSTI]

    Santarpia, Joshua

    2001-01-01

    An investigation of the kinematic and electrical properties of the 9-10 June, 1998 Red River Mesoscale Convective System (MCS), as observed by the NOAA P3 Tail Radar and the National Lightning Detection Network, is presented. This system exhibits...

  8. A climatology, synoptic assessment, and thermodynamic evaluation for cloud-to-ground lightning in Georgia: a study for the 1996 Summer Olympics 

    E-Print Network [OSTI]

    Livingston, Eric Scott

    1995-01-01

    of Athens, Georgia based on the thermodynamic variables. At the inland locations, no pattern was found in the spatial distribution of cloud-to-ground lightning: the strike locations were random. At the coastal location, an inland maximum in ground flash...

  9. Coarse-graining DNA for simulations of DNA nanotechnology

    E-Print Network [OSTI]

    Jonathan P. K. Doye; Thomas E. Ouldridge; Ard A. Louis; Flavio Romano; Petr Sulc; Christian Matek; Benedict E. K. Snodin; Lorenzo Rovigatti; John S. Schreck; Ryan M. Harrison; William P. J. Smith

    2013-08-18

    To simulate long time and length scale processes involving DNA it is necessary to use a coarse-grained description. Here we provide an overview of different approaches to such coarse graining, focussing on those at the nucleotide level that allow the self-assembly processes associated with DNA nanotechnology to be studied. OxDNA, our recently-developed coarse-grained DNA model, is particularly suited to this task, and has opened up this field to systematic study by simulations. We illustrate some of the range of DNA nanotechnology systems to which the model is being applied, as well as the insights it can provide into fundamental biophysical properties of DNA.

  10. Coarse-graining DNA for simulations of DNA nanotechnology

    E-Print Network [OSTI]

    Doye, Jonathan P K; Louis, Ard A; Romano, Flavio; Sulc, Petr; Matek, Christian; Snodin, Benedict E K; Rovigatti, Lorenzo; Schreck, John S; Harrison, Ryan M; Smith, William P J

    2013-01-01

    To simulate long time and length scale processes involving DNA it is necessary to use a coarse-grained description. Here we provide an overview of different approaches to such coarse graining, focussing on those at the nucleotide level that allow the self-assembly processes associated with DNA nanotechnology to be studied. OxDNA, our recently-developed coarse-grained DNA model, is particularly suited to this task, and has opened up this field to systematic study by simulations. We illustrate some of the range of DNA nanotechnology systems to which the model is being applied, as well as the insights it can provide into fundamental biophysical properties of DNA.

  11. DNA polymerase having modified nucleotide binding site for DNA sequencing

    DOE Patents [OSTI]

    Tabor, S.; Richardson, C.

    1997-03-25

    A modified gene encoding a modified DNA polymerase is disclosed. The modified polymerase incorporates dideoxynucleotides at least 20-fold better compared to the corresponding deoxynucleotides as compared with the corresponding naturally-occurring DNA polymerase. 6 figs.

  12. Searching for DNA Lesions: Structural Evidence for Lower- and Higher-Affinity DNA Binding Conformations of Human Alkyladenine DNA Glycosylase

    E-Print Network [OSTI]

    Drennan, Catherine L.

    To efficiently repair DNA, human alkyladenine DNA glycosylase (AAG) must search the million-fold excess of unmodified DNA bases to find a handful of DNA lesions. Such a search can be facilitated by the ability of glycosylases, ...

  13. DNA Structural Nanotechnology Duke University

    E-Print Network [OSTI]

    Reif, John H.

    DNA Structural Nanotechnology John Reif Duke University Graduate Students: Harish Chandran&Caltech Tube Lattices #12;Ned Seeman New York University, USA Ned Seeman: Father of DNA Nanotechnology His Initial Ideas & Motivation for DNA Nanotechnology #12;Cube Chen & Seeman, Nature350:631 (1991) Truncated

  14. Docking-dependent regulation of checkpoint kinase chk1 by the growth regulator p21WAF1 

    E-Print Network [OSTI]

    Toh, Yew Kwang

    2009-01-01

    Checkpoint kinase 1 (Chk1) is a key player in the DNA damage response signalling pathway and the mode of Chk1 activation whereby it undergoes ATRdependent phosphorylation at Ser317 and Ser345 is well characterised. It ...

  15. Fleet DNA (Presentation)

    SciTech Connect (OSTI)

    Walkokwicz, K.; Duran, A.

    2014-06-01

    The Fleet DNA project objectives include capturing and quantifying drive cycle and technology variation for the multitude of medium- and heavy-duty vocations; providing a common data storage warehouse for medium- and heavy-duty vehicle fleet data across DOE activities and laboratories; and integrating existing DOE tools, models, and analyses to provide data-driven decision making capabilities. Fleet DNA advantages include: for Government - providing in-use data for standard drive cycle development, R&D, tech targets, and rule making; for OEMs - real-world usage datasets provide concrete examples of customer use profiles; for fleets - vocational datasets help illustrate how to maximize return on technology investments; for Funding Agencies - ways are revealed to optimize the impact of financial incentive offers; and for researchers -a data source is provided for modeling and simulation.

  16. DNA waves and water

    E-Print Network [OSTI]

    L. Montagnier; J. Aissa; E. Del Giudice; C. Lavallee; A. Tedeschi; G. Vitiello

    2010-12-23

    Some bacterial and viral DNA sequences have been found to induce low frequency electromagnetic waves in high aqueous dilutions. This phenomenon appears to be triggered by the ambient electromagnetic background of very low frequency. We discuss this phenomenon in the framework of quantum field theory. A scheme able to account for the observations is proposed. The reported phenomenon could allow to develop highly sensitive detection systems for chronic bacterial and viral infections.

  17. DNA | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIX E LISTStar EnergyLawler,CoalConcordiaConsumerLEDSEnergyDMS Company Ltd Jump to:DNA Jump

  18. Method for assaying clustered DNA damages

    DOE Patents [OSTI]

    Sutherland, Betsy M.

    2004-09-07

    Disclosed is a method for detecting and quantifying clustered damages in DNA. In this method, a first aliquot of the DNA to be tested for clustered damages with one or more lesion-specific cleaving reagents under conditions appropriate for cleavage of the DNA to produce single-strand nicks in the DNA at sites of damage lesions. The number average molecular length (Ln) of double stranded DNA is then quantitatively determined for the treated DNA. The number average molecular length (Ln) of double stranded DNA is also quantitatively determined for a second, untreated aliquot of the DNA. The frequency of clustered damages (.PHI..sub.c) in the DNA is then calculated.

  19. Genome-Wide Identification and 3D Modeling of Proteins involved in DNA Damage Recognition and Repair (Final Report)

    SciTech Connect (OSTI)

    Ruben A. Abagyan, PhD

    2004-04-15

    OAK-B135 DNA Damage Recognition and Repair (DDR and R) proteins play a critical role in cellular responses to low-dose radiation and are associated with cancer. the authors have performed a systematic, genome-wide computational analysis of genomic data for human genes involved in the DDR and R process. The significant achievements of this project include: (1) Construction of the computational pipeline for searching DDR and R genes, building and validation of 3D models of proteins involved in DDR and R; (2) Functional and structural annotation of the 3D models and generation of comprehensive lists of suggested knock-out mutations; (3) Important improvement of macromolecular docking technology and its application to predict the DNA-Protein complex conformation; (4) Development of a new algorithm for improved analysis of high-density oligonucleotide arrays for gene expression profiling; (5) Construction and maintenance of the DNA Damage Recognition and Repair Database; and (6) Producing 14 research papers (10 published and 4 in preparation).

  20. Annual Report: 2010-2011 Storm Season Sampling For NON-DRY DOCK STORMWATER MONITORING FOR PUGET SOUND NAVAL SHIPYARD, BREMERTON, WA

    SciTech Connect (OSTI)

    Brandenberger, Jill M.; Metallo, David; Johnston, Robert K.; Gebhardt, Christine; Hsu, Larry

    2012-09-01

    This interim report summarizes the stormwater monitoring conducted for non-dry dock outfalls in both the confined industrial area and the residential areas of Naval Base Kitsap within the Puget Sound Naval Shipyard (referred to as the Shipyard). This includes the collection, analyses, and descriptive statistics for stormwater sampling conducted from November 2010 through April 2011. Seven stormwater basins within the Shipyard were sampled during at least three storm events to characterize non-dry dock stormwater discharges at selected stormwater drains located within the facility. This serves as the Phase I component of the project and Phase II is planned for the 2011-2012 storm season. These data will assist the Navy, USEPA, Ecology and other stakeholders in understanding the nature and condition of stormwater discharges from the Shipyard and inform the permitting process for new outfall discharges. The data from Phase I was compiled with current stormwater data available from the Shipyard, Sinclair/Dyes Inlet watershed, and Puget Sound in order to support technical investigations for the Draft NPDES permit. The permit would require storm event sampling at selected stormwater drains located within the Shipyard. However, the data must be considered on multiple scales to truly understand potential impairments to beneficial uses within Sinclair and Dyes Inlets.

  1. Normalized cDNA libraries

    DOE Patents [OSTI]

    Soares, M.B.; Efstratiadis, A.

    1997-06-10

    This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3{prime} noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library. 4 figs.

  2. Normalized cDNA libraries

    DOE Patents [OSTI]

    Soares, Marcelo B. (New York, NY); Efstratiadis, Argiris (Englewood, NJ)

    1997-01-01

    This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3' noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library.

  3. Sequence independent amplification of DNA

    DOE Patents [OSTI]

    Bohlander, S.K.

    1998-03-24

    The present invention is a rapid sequence-independent amplification procedure (SIA). Even minute amounts of DNA from various sources can be amplified independent of any sequence requirements of the DNA or any a priori knowledge of any sequence characteristics of the DNA to be amplified. This method allows, for example, the sequence independent amplification of microdissected chromosomal material and the reliable construction of high quality fluorescent in situ hybridization (FISH) probes from YACs or from other sources. These probes can be used to localize YACs on metaphase chromosomes but also--with high efficiency--in interphase nuclei. 25 figs.

  4. Topics in Forensic DNA Analysis &

    E-Print Network [OSTI]

    chemistry from the University of Virginia. His dissertation research, which was conducted at the FBI Academy guest of the FBI's Scientific Working Group on DNA Analysis Methods (SWGDAM) and a member

  5. Genome-Wide Identification and 3D Modeling of Proteins involved in DNA Damage Recognition and Repair (Final Report)

    SciTech Connect (OSTI)

    Abagyan, Ruben; An, Jianghong

    2005-08-12

    DNA Damage Recognition and Repair (DDR&R) proteins play a critical role in cellular responses to low-dose radiation and are associated with cancer. We have performed a systematic, genome-wide computational analysis of genomic data for human genes involved in the DDR&R process. The significant achievements of this project include: 1) Construction of the computational pipeline for searching DDR&R genes, building and validation of 3D models of proteins involved in DDR&R; 2) Functional and structural annotation of the 3D models and generation of comprehensive lists of suggested knock-out mutations; and the development of a method to predict the effects of mutations. Large scale testing of technology to identify novel small binding pockets in protein structures leading to new DDRR inhibitor strategies 3) Improvements of macromolecular docking technology (see the CAPRI 1-3 and 4-5 results) 4) Development of a new algorithm for improved analysis of high-density oligonucleotide arrays for gene expression profiling; 5) Construction and maintenance of the DNA Damage Recognition and Repair Database; 6) Producing 15 research papers (12 published and 3 in preparation).

  6. Characterization of nanoparticle-DNA conjugate and control of DNA conformation on particle surface

    E-Print Network [OSTI]

    Park, Sunho, 1976-

    2009-01-01

    Nano-science has exploited the hybridization and de-hybridization phenomena of DNA which are one of its fundamental functions. In particular, conjugates of gold nanoparticles and DNA (Au NP-DNA) have been extensively ...

  7. Regulation of DNA damage tolerance : studies of the translesion synthesis DNA ploymerase eta in Saccharomyces cerevisiae

    E-Print Network [OSTI]

    Woodruff, Rachel Van Etten

    2008-01-01

    All organisms must control the effects of DNA damage to protect the integrity of their genomes. In addition to DNA repair, this requires DNA damage tolerance pathways, which allow the continuation of essential processes ...

  8. Magnetic tweezers to studyMagnetic tweezers to studyMagnetic tweezers to studyMagnetic tweezers to study DNA motorsDNA motorsDNA motorsDNA motors

    E-Print Network [OSTI]

    Ritort, Felix

    to study DNA motorsDNA motorsDNA motorsDNA motors MariaMariaMariaMaria MañosasMañosasMañosasMañosas Ritort) · Applications: 1. Tracking DNA motors: (i) Helicases (ii) Annealing motor 2. Studying a multiprotein system: DNA hexamers (Dong et al, JBC 1995) Tracking DNA motors: (i) Helicases #12;Passive: helicase behaves

  9. The Structure of DNA within Cationic Lipid/DNA Complexes

    E-Print Network [OSTI]

    Braun, Chad S.; Jas, Gouri S.; Choosakoonriang, Sirirat; Koe, Gary S.; Smith, Janet G.; Middaugh, C. Russell

    2003-02-01

    hexagonal phase of cationic liposome-DNA complexes related to DNA release and delivery. Science. 281:78–81. Koppel, D. E. 1972. Analysis of macromolecular polydispersity in inten- sity correlation spectroscopy: the method of cumulants. J. Chem. Phys. 57:4814..., DOTAP, 1,2-dioleoyl-sn-glycero-3-phosphatidyletha- nolamine, and cholesterol were purchased from Avanti Polar Lipids (Alabaster, AL). Poly(dG) Æ poly(dC) (4 kbp), poly(dA) Æ poly(dT) (;229 bp), poly(dGdC) Æ poly(dGdC) (724 bp), and poly(dAdT) Æ poly(d...

  10. DNA repair decline during mouse spermiogenesis results in the accumulation of heritable DNA damage

    E-Print Network [OSTI]

    Marchetti, Francesco

    2008-01-01

    male germ cells handle DNA damage? Toxicol. Appl. Pharmacol.strand breaks and DNA base damage at different cellularrelationship to genetic damage, Mutat. Res. 216 (1989) 221-

  11. DNA Repair Decline During Mouse Spermiogenesis Results in the Accumulation of Heritable DNA Damage

    E-Print Network [OSTI]

    Marchetti, Francesco

    2008-01-01

    male germ cells handle DNA damage? Toxicol. Appl. Pharmacol.strand breaks and DNA base damage at different cellularrelationship to genetic damage, Mutat. Res. 216 (1989) 221-

  12. The Initiation of Bacterial DNA Replication

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    for this system. Instead, DnaA forms an open right-handed helix. In addition, the architecture indicates that this AAA+ superhelix will wrap coils of the DNA around its exterior,...

  13. Micropatterned cell arrays for detecting DNA damage

    E-Print Network [OSTI]

    Mittal, Sukant

    2008-01-01

    Numerous agents are capable of interacting with DNA and damaging it. Permanent changes in the DNA structure can be both mutagenic and cytotoxic; therefore, methods to measure the susceptibility of cells to mutations are ...

  14. Towards Privacy Preserving of Forensic DNA Databases 

    E-Print Network [OSTI]

    Liu, Sanmin

    2012-02-14

    Protecting privacy of individuals is critical for forensic genetics. In a kinship/identity testing, related DNA profiles between user's query and the DNA database need to be extracted. However, unrelated profiles cannot be revealed to each other...

  15. Chromosome specific repetitive DNA sequences

    DOE Patents [OSTI]

    Moyzis, Robert K. (Los Alamos, NM); Meyne, Julianne (Los Alamos, NM)

    1991-01-01

    A method is provided for determining specific nucleotide sequences useful in forming a probe which can identify specific chromosomes, preferably through in situ hybridization within the cell itself. In one embodiment, chromosome preferential nucleotide sequences are first determined from a library of recombinant DNA clones having families of repetitive sequences. Library clones are identified with a low homology with a sequence of repetitive DNA families to which the first clones respectively belong and variant sequences are then identified by selecting clones having a pattern of hybridization with genomic DNA dissimilar to the hybridization pattern shown by the respective families. In another embodiment, variant sequences are selected from a sequence of a known repetitive DNA family. The selected variant sequence is classified as chromosome specific, chromosome preferential, or chromosome nonspecific. Sequences which are classified as chromosome preferential are further sequenced and regions are identified having a low homology with other regions of the chromosome preferential sequence or with known sequences of other family me This invention is the result of a contract with the Department of Energy (Contract No. W-7405-ENG-36).

  16. The Future of Forensic DNA

    E-Print Network [OSTI]

    History and Mission · National Institute of Standards and Technology (NIST) was created in 1901The Future of Forensic DNA John M. Butler, PhD National Institute of Standards and Technology.S. Department of Commerce with a mission to develop and promote measurement, standards, and technology

  17. Origami DNA model Mountain fold

    E-Print Network [OSTI]

    Csürös, Miklós

    Origami DNA model Mountain fold Solid lines are "mountains" and are to be folded away from you with the peak pointing towards you. 1. Fold all solid lines going lengthwise down the page into "mountain folds fold 2. Fold all dashed lines going lengthwise down the page into "valley folds". Mountain folds along

  18. The Future of Forensic DNA

    E-Print Network [OSTI]

    ;Checks and Controls on DNA Results Community FBI Quality Assurance Standards (and interlaboratory studies Washington D.C. Dulles Airport Reagan National Airport BWI Airport NIST FBI Lab Baltimore, MD Richmond, VA Materials (SRMs) Helps meet FBI QAS and ISO 17025 requirements Traceable standards to ensure accurate

  19. DNA Mixture Interpretation & Statistical Analysis

    E-Print Network [OSTI]

    of Standards and Technology Gaithersburg, Maryland John M. Butler CIB Forensic Science Center Training Seminar Mixture Workshop This workshop is for analysts, technical reviewers and technical leaders performing) National recommendations of the technical UK DNA working group on mixture interpretation for the NDNAD

  20. DNA sequencing protocols BN Danforth

    E-Print Network [OSTI]

    Danforth, Bryan Nicholas

    in the degradation of DNA. (7) Spin the tubes down at the end to remove condensation from tops of tubes. B. Extraction and RNA digestion. NOTE: We now skip the Rnase step. You will need: Phenol and gently invert several times. This step removes the phenol from the previous extraction. Spin

  1. Biological Physics of DNA Typeset by FoilTEX 1

    E-Print Network [OSTI]

    Potsdam, Universität

    fragments to ssDNA Labelling: eg radioact probe fragm & Xray film http;Packaging of DNA in bacteria 11 #12;DNA melting 12 #12;Polymerase chain reaction Heating dsDNA sample 2

  2. DNA extraction techniques for DNA barcoding of minute gall-inhabiting wasps

    E-Print Network [OSTI]

    extraction methods were compared to determine their efficacy in isolating DNA. Success of each methodDNA extraction techniques for DNA barcoding of minute gall-inhabiting wasps GUDRUN DITTRICH, South Africa Abstract DNA extraction from minute hymenopterans and their larvae is difficult

  3. DNA Word Design Strategy for Creating Sets of Non-interacting Oligonucleotides for DNA Microarrays

    E-Print Network [OSTI]

    DNA Word Design Strategy for Creating Sets of Non-interacting Oligonucleotides for DNA Microarrays mismatches with the complements of all the other members in the set. These "DNA word" sets are denoted as nbm. To achieve good discrimination between each DNA word in each set generated using the template-map strategy

  4. Probe and method for DNA detection

    DOE Patents [OSTI]

    Yeh, Hsin-Chih; Werner, James Henry; Sharma, Jaswinder Kumar; Martinez, Jennifer Suzanne

    2013-07-02

    A hybridization probe containing two linear strands of DNA lights up upon hybridization to a target DNA using silver nanoclusters that have been templated onto one of the DNA strands. Hybridization induces proximity between the nanoclusters on one strand and an overhang on the other strand, which results in enhanced fluorescence emission from the nanoclusters.

  5. MATERIALS AND METHODS 1) DNA extraction

    E-Print Network [OSTI]

    Collins, Gary S.

    MATERIALS AND METHODS 1) DNA extraction · DNA was extracted from the ileo-cecal nodes of 475 Holstein cows from two herds using the Qiagen DNA extraction kit (Valencia, CA). 2) Map detection · Map was extracted from ileo-cecal nodes using Ambion's MagMAX Total Nucleic Acid Isolation kit (Austin, TX

  6. Binding of undamaged double stranded DNA to vaccinia virus uracil-DNA glycosylase

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Schormann, Norbert; Banerjee, Surajit; Ricciardi, Robert; Chattopadhyay, Debasish

    2015-06-02

    Background: Uracil-DNA glycosylases are evolutionarily conserved DNA repair enzymes. However, vaccinia virus uracil-DNA glycosylase (known as D4), also serves as an intrinsic and essential component of the processive DNA polymerase complex during DNA replication. In this complex D4 binds to a unique poxvirus specific protein A20 which tethers it to the DNA polymerase. At the replication fork the DNA scanning and repair function of D4 is coupled with DNA replication. So far, DNA-binding to D4 has not been structurally characterized. Results: This manuscript describes the first structure of a DNA-complex of a uracil-DNA glycosylase from the poxvirus family. This alsomore »represents the first structure of a uracil DNA glycosylase in complex with an undamaged DNA. In the asymmetric unit two D4 subunits bind simultaneously to complementary strands of the DNA double helix. Each D4 subunit interacts mainly with the central region of one strand. DNA binds to the opposite side of the A20-binding surface on D4. In comparison of the present structure with the structure of uracil-containing DNA-bound human uracil-DNA glycosylase suggests that for DNA binding and uracil removal D4 employs a unique set of residues and motifs that are highly conserved within the poxvirus family but different in other organisms. Conclusion: The first structure of D4 bound to a truly non-specific undamaged double-stranded DNA suggests that initial binding of DNA may involve multiple non-specific interactions between the protein and the phosphate backbone.« less

  7. LIGHTNING SYMPOSIUM November 16

    E-Print Network [OSTI]

    Taylor, Steven J.

    Geothermal Resource Mapping 11:45 a.m. to 1:15 p.m. Lunch on your own 1:15 p.m. Session 2A Andrew Osmosis ­ An Emerging Desalination Technique Tom Holm ISWS PAHs and Biochar: Sorption and Bioava

  8. ARM - Measurement - Lightning stroke

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Comments?govInstrumentsnoaacrnBarrow, Alaska Outreach Home Roomparticle

  9. Microfluidic DNA sample preparation method and device

    DOE Patents [OSTI]

    Krulevitch, Peter A. (Pleasanton, CA); Miles, Robin R. (Danville, CA); Wang, Xiao-Bo (San Diego, CA); Mariella, Raymond P. (Danville, CA); Gascoyne, Peter R. C. (Bellaire, TX); Balch, Joseph W. (Livermore, CA)

    2002-01-01

    Manipulation of DNA molecules in solution has become an essential aspect of genetic analyses used for biomedical assays, the identification of hazardous bacterial agents, and in decoding the human genome. Currently, most of the steps involved in preparing a DNA sample for analysis are performed manually and are time, labor, and equipment intensive. These steps include extraction of the DNA from spores or cells, separation of the DNA from other particles and molecules in the solution (e.g. dust, smoke, cell/spore debris, and proteins), and separation of the DNA itself into strands of specific lengths. Dielectrophoresis (DEP), a phenomenon whereby polarizable particles move in response to a gradient in electric field, can be used to manipulate and separate DNA in an automated fashion, considerably reducing the time and expense involved in DNA analyses, as well as allowing for the miniaturization of DNA analysis instruments. These applications include direct transport of DNA, trapping of DNA to allow for its separation from other particles or molecules in the solution, and the separation of DNA into strands of varying lengths.

  10. Electromagnetic Signals from Bacterial DNA

    E-Print Network [OSTI]

    A. Widom; J. Swain; Y. N. Srivastava; S. Sivasubramanian

    2012-02-09

    Chemical reactions can be induced at a distance due to the propagation of electromagnetic signals during intermediate chemical stages. Although is is well known at optical frequencies, e.g. photosynthetic reactions, electromagnetic signals hold true for muck lower frequencies. In E. coli bacteria such electromagnetic signals can be generated by electric transitions between energy levels describing electrons moving around DNA loops. The electromagnetic signals between different bacteria within a community is a "wireless" version of intercellular communication found in bacterial communities connected by "nanowires". The wireless broadcasts can in principle be of both the AM and FM variety due to the magnetic flux periodicity in electron energy spectra in bacterial DNA orbital motions.

  11. Fleet DNA Project (Fact Sheet)

    SciTech Connect (OSTI)

    Not Available

    2012-10-01

    The Fleet DNA Project - designed by the U.S. Department of Energy's National Renewable Energy Laboratory (NREL) in partnership with Oak Ridge National Laboratory - aims to accelerate the evolution of advanced vehicle development and support the strategic deployment of market-ready technologies that reduce costs, fuel consumption, and emissions. At the heart of the Fleet DNA Project is a clearinghouse of medium- and heavy-duty commercial fleet transportation data for optimizing the design of advanced vehicle technologies or for selecting a given technology to invest in. An easy-to-access online database will help vehicle manufacturers and fleets understand the broad operational range for many of today's commercial vehicle vocations.

  12. Channel plate for DNA sequencing

    DOE Patents [OSTI]

    Douthart, R.J.; Crowell, S.L.

    1998-01-13

    This invention is a channel plate that facilitates data compaction in DNA sequencing. The channel plate has a length, a width and a thickness, and further has a plurality of channels that are parallel. Each channel has a depth partially through the thickness of the channel plate. Additionally an interface edge permits electrical communication across an interface through a buffer to a deposition membrane surface. 15 figs.

  13. The dynamic interplay between DNA damage and metabolism : the metabolic fate and transport of DNA lesions and novel DNA damage derived from intermediary metabolism

    E-Print Network [OSTI]

    Jumpathong, Watthanachai

    2014-01-01

    The work presented in this thesis explores two novel and complementary facets of endogenous DNA damage: the development of biomarkers of inflammation based on metabolites of DNA damage products and the formation of DNA ...

  14. Photochemistry of psoralen-DNA adducts, biological effects of psoralen-DNA adducts, applications of psoralen-DNA photochemistry

    SciTech Connect (OSTI)

    Shi, Yun-bo

    1988-03-01

    This thesis consists of three main parts and totally eight chapters. In Part I, The author will present studies on the photochemistry of psoralen-DNA adducts, specifically, the wavelength dependencies for the photoreversals of thymidine-HMT (4'-hydroxymethyl-4, 5', 8-trimenthylpsoralen) monoadducts and diadduct and the same adducts incorporated in DNA helices and the wavelength dependecies for the photocrossslinking of thymidine-HMT monoadducts in double-stranded helices. In Part II, The author will report some biological effects of psoralen-DNA adducts, i.e., the effects on double-stranded DNA stability, DNA structure, and transcription by E. coli and T7 RNA polymerases. Finally, The author will focus on the applications of psoralen-DNA photochemistry to investigation of protein-DNA interaction during transcription, which includes the interaction of E. coli and T7 RNA polymerases with DNA in elongation complexes arrested at specific psoralen-DNA adduct sites as revealed by DNase I footprinting experiments. 123 refs., 52 figs., 12 tabs.

  15. DNA Sequencing Using capillary Electrophoresis

    SciTech Connect (OSTI)

    Dr. Barry Karger

    2011-05-09

    The overall goal of this program was to develop capillary electrophoresis as the tool to be used to sequence for the first time the Human Genome. Our program was part of the Human Genome Project. In this work, we were highly successful and the replaceable polymer we developed, linear polyacrylamide, was used by the DOE sequencing lab in California to sequence a significant portion of the human genome using the MegaBase multiple capillary array electrophoresis instrument. In this final report, we summarize our efforts and success. We began our work by separating by capillary electrophoresis double strand oligonucleotides using cross-linked polyacrylamide gels in fused silica capillaries. This work showed the potential of the methodology. However, preparation of such cross-linked gel capillaries was difficult with poor reproducibility, and even more important, the columns were not very stable. We improved stability by using non-cross linked linear polyacrylamide. Here, the entangled linear chains could move when osmotic pressure (e.g. sample injection) was imposed on the polymer matrix. This relaxation of the polymer dissipated the stress in the column. Our next advance was to use significantly lower concentrations of the linear polyacrylamide that the polymer could be automatically blown out after each run and replaced with fresh linear polymer solution. In this way, a new column was available for each analytical run. Finally, while testing many linear polymers, we selected linear polyacrylamide as the best matrix as it was the most hydrophilic polymer available. Under our DOE program, we demonstrated initially the success of the linear polyacrylamide to separate double strand DNA. We note that the method is used even today to assay purity of double stranded DNA fragments. Our focus, of course, was on the separation of single stranded DNA for sequencing purposes. In one paper, we demonstrated the success of our approach in sequencing up to 500 bases. Other application papers of sequencing up to this level were also published in the mid 1990's. A major interest of the sequencing community has always been read length. The longer the sequence read per run the more efficient the process as well as the ability to read repeat sequences. We therefore devoted a great deal of time to studying the factors influencing read length in capillary electrophoresis, including polymer type and molecule weight, capillary column temperature, applied electric field, etc. In our initial optimization, we were able to demonstrate, for the first time, the sequencing of over 1000 bases with 90% accuracy. The run required 80 minutes for separation. Sequencing of 1000 bases per column was next demonstrated on a multiple capillary instrument. Our studies revealed that linear polyacrylamide produced the longest read lengths because the hydrophilic single strand DNA had minimal interaction with the very hydrophilic linear polyacrylamide. Any interaction of the DNA with the polymer would lead to broader peaks and lower read length. Another important parameter was the molecular weight of the linear chains. High molecular weight (> 1 MDA) was important to allow the long single strand DNA to reptate through the entangled polymer matrix. In an important paper, we showed an inverse emulsion method to prepare reproducibility linear polyacrylamide polymer with an average MWT of 9MDa. This approach was used in the polymer for sequencing the human genome. Another critical factor in the successful use of capillary electrophoresis for sequencing was the sample preparation method. In the Sanger sequencing reaction, high concentration of salts and dideoxynucleotide remained. Since the sample was introduced to the capillary column by electrokinetic injection, these salt ions would be favorably injected into the column over the sequencing fragments, thus reducing the signal for longer fragments and hence reading read length. In two papers, we examined the role of individual components from the sequencing reaction and then developed a protocol to reduce the deleterio

  16. Assembling semiconductor nanocomposites using DNA replication technologies.

    SciTech Connect (OSTI)

    Heimer, Brandon W.; Crown, Kevin K.; Bachand, George David

    2005-11-01

    Deoxyribonucleic acid (DNA) molecules represent Nature's genetic database, encoding the information necessary for all cellular processes. From a materials engineering perspective, DNA represents a nanoscale scaffold with highly refined structure, stability across a wide range of environmental conditions, and the ability to interact with a range of biomolecules. The ability to mass-manufacture functionalized DNA strands with Angstrom-level resolution through DNA replication technology, however, has not been explored. The long-term goal of the work presented in this report is focused on exploiting DNA and in vitro DNA replication processes to mass-manufacture nanocomposite materials. The specific objectives of this project were to: (1) develop methods for replicating DNA strands that incorporate nucleotides with ''chemical handles'', and (2) demonstrate attachment of nanocrystal quantum dots (nQDs) to functionalized DNA strands. Polymerase chain reaction (PCR) and primer extension methodologies were used to successfully synthesize amine-, thiol-, and biotin-functionalized DNA molecules. Significant variability in the efficiency of modified nucleotide incorporation was observed, and attributed to the intrinsic properties of the modified nucleotides. Noncovalent attachment of streptavidin-coated nQDs to biotin-modified DNA synthesized using the primer extension method was observed by epifluorescence microscopy. Data regarding covalent attachment of nQDs to amine- and thiol-functionalized DNA was generally inconclusive; alternative characterization tools are necessary to fully evaluate these attachment methods. Full realization of this technology may facilitate new approaches to manufacturing materials at the nanoscale. In addition, composite nQD-DNA materials may serve as novel recognition elements in sensor devices, or be used as diagnostic tools for forensic analyses. This report summarizes the results obtained over the course of this 1-year project.

  17. Enhancing the DNA Patent Database

    SciTech Connect (OSTI)

    Walters, LeRoy B.

    2008-02-18

    Final Report on Award No. DE-FG0201ER63171 Principal Investigator: LeRoy B. Walters February 18, 2008 This project successfully completed its goal of surveying and reporting on the DNA patenting and licensing policies at 30 major U.S. academic institutions. The report of survey results was published in the January 2006 issue of Nature Biotechnology under the title “The Licensing of DNA Patents by US Academic Institutions: An Empirical Survey.” Lori Pressman was the lead author on this feature article. A PDF reprint of the article will be submitted to our Program Officer under separate cover. The project team has continued to update the DNA Patent Database on a weekly basis since the conclusion of the project. The database can be accessed at dnapatents.georgetown.edu. This database provides a valuable research tool for academic researchers, policymakers, and citizens. A report entitled Reaping the Benefits of Genomic and Proteomic Research: Intellectual Property Rights, Innovation, and Public Health was published in 2006 by the Committee on Intellectual Property Rights in Genomic and Protein Research and Innovation, Board on Science, Technology, and Economic Policy at the National Academies. The report was edited by Stephen A. Merrill and Anne-Marie Mazza. This report employed and then adapted the methodology developed by our research project and quoted our findings at several points. (The full report can be viewed online at the following URL: http://www.nap.edu/openbook.php?record_id=11487&page=R1). My colleagues and I are grateful for the research support of the ELSI program at the U.S. Department of Energy.

  18. DNA

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration would like submit theCovalent Bonding Low-Cost Ground8 GasDEVELOPMENTS E P I IT h it cdrives

  19. DNA

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    blueprint of a bacterium's "molecular machinery," showing how bacterial immune systems fight off the viruses that infect them. By tracking down how bacterial defense systems work,...

  20. Base Excision by Thymine DNA Glycosylase Mediates DNA-Directed Cytotoxicity of 5-Fluorouracil 

    E-Print Network [OSTI]

    Selfridge J.; Schar P.; Lettieri T.; Schuermann D.; Saito Y.; Focke F.; Kunz C.

    2009-04-01

    5-Fluorouracil (5-FU), a chemotherapeutic drug commonly used in cancer treatment, imbalances nucleotide pools, thereby favoring misincorporation of uracil and 5-FU into genomic DNA. The processing of these bases by DNA repair activities was proposed...

  1. Protein-DNA Interactions Determine the Shapes of DNA Toroids Condensed in Virus Capsids

    E-Print Network [OSTI]

    Podgornik, Rudolf

    Protein-DNA Interactions Determine the Shapes of DNA Toroids Condensed in Virus Capsids Ame (13), or the virus capsid itself (14­16), either upon addition of spermine (Spm4þ ) or in a monovalent

  2. DNA binding specificity of the p73 DNA-binding domain

    E-Print Network [OSTI]

    Tse, Pui Wah

    2011-01-01

    of DNA recognition by p53 tetramers. Mol Cell 22, 741-753.site as a self-assembled tetramer. Structure 18, 246- Chene,structure of a p53 core tetramer bound to DNA. Oncogene 28,

  3. DNA-guided nanoparticle assemblies

    DOE Patents [OSTI]

    Gang, Oleg; Nykypanchuk, Dmytro; Maye, Mathew; van der Lelie, Daniel

    2013-07-16

    In some embodiments, DNA-capped nanoparticles are used to define a degree of crystalline order in assemblies thereof. In some embodiments, thermodynamically reversible and stable body-centered cubic (bcc) structures, with particles occupying <.about.10% of the unit cell, are formed. Designs and pathways amenable to the crystallization of particle assemblies are identified. In some embodiments, a plasmonic crystal is provided. In some aspects, a method for controlling the properties of particle assemblages is provided. In some embodiments a catalyst is formed from nanoparticles linked by nucleic acid sequences and forming an open crystal structure with catalytically active agents attached to the crystal on its surface or in interstices.

  4. DNA: The Strand that Connects Us All

    SciTech Connect (OSTI)

    Kaplan, Matt (University of Arizona Genetics Core) [University of Arizona Genetics Core

    2011-03-29

    Learn how the methods and discoveries of human population genetics are applied for personal genealogical reconstruction and anthropological testing. Dr. Kaplan starts with a short general review of human genetics and the biology behind this form of DNA testing. He looks at how DNA testing is performed and how samples are processed in the University of Arizona laboratory. He also examines examples of personal genealogical results from Family Tree DNA and personal anthropological results from the Genographic Project. Finally, he describes the newest project in the UA laboratory, the DNA Shoah Project.

  5. DNA sequencing using fluorescence background electroblotting membrane

    DOE Patents [OSTI]

    Caldwell, K.D.; Chu, T.J.; Pitt, W.G.

    1992-05-12

    A method for the multiplex sequencing on DNA is disclosed which comprises the electroblotting or specific base terminated DNA fragments, which have been resolved by gel electrophoresis, onto the surface of a neutral non-aromatic polymeric microporous membrane exhibiting low background fluorescence which has been surface modified to contain amino groups. Polypropylene membranes are preferably and the introduction of amino groups is accomplished by subjecting the membrane to radio or microwave frequency plasma discharge in the presence of an aminating agent, preferably ammonia. The membrane, containing physically adsorbed DNA fragments on its surface after the electroblotting, is then treated with crosslinking means such as UV radiation or a glutaraldehyde spray to chemically bind the DNA fragments to the membrane through amino groups contained on the surface. The DNA fragments chemically bound to the membrane are subjected to hybridization probing with a tagged probe specific to the sequence of the DNA fragments. The tagging may be by either fluorophores or radioisotopes. The tagged probes hybridized to the target DNA fragments are detected and read by laser induced fluorescence detection or autoradiograms. The use of aminated low fluorescent background membranes allows the use of fluorescent detection and reading even when the available amount of DNA to be sequenced is small. The DNA bound to the membranes may be reprobed numerous times. No Drawings

  6. SnapShot: DNA Polymerases II Mammals

    E-Print Network [OSTI]

    Foti, James J.

    DNA polymerases ensure the faithful duplication of genetic information inside the nuclease and mitochondria of eukaryotic cells and the nucleoid of prokaryotic cells. These remarkable enzymes synthesize polynucleotide ...

  7. DNA Profiling Using Solid-State Nanopores: Detection of DNA-Binding

    E-Print Network [OSTI]

    Meller, Amit

    a 3.5 nm pore results from threading of a dye-intercalated DNA molecule, as compared to the typical for drug development, necessitating new in vitro methods for rapid and low-cost assessment of the binding molecules, which give the DNA/intercalator complex a bulkier structure than that of native DNA. Furthermore

  8. Ancient DNA Chronology within Sediment Deposits: Are Paleobiological Reconstructions Possible and Is DNA Leaching a Factor?

    E-Print Network [OSTI]

    Nielsen, Rasmus

    Ancient DNA Chronology within Sediment Deposits: Are Paleobiological Reconstructions Possible reported the successful extraction of ancient DNA (aDNA) from both frozen and nonfrozen sediments (even sediments up to 3300 years old at 2 cave sites in the North Island of New Zealand. These sites are ideal

  9. Dellaporta DNA Extraction Citation: Stephen L. Dellaporta,Jonathan Wood , James B. Hicks. A plant DNA

    E-Print Network [OSTI]

    Wurtele, Eve Syrkin

    1 Dellaporta DNA Extraction Citation: Stephen L. Dellaporta,Jonathan Wood , James B. Hicks. A plant supernatant and lightly dry DNA pellets by inverting the tubes on paper towels for 10 min. #12;4 12. Redissolve each DNA pellet with 0.7 mL EB2. May need to let sit overnight at 4°C if having trouble dissolving

  10. Discovery of Daemons Makes Power Generation by Daemon-Assisted Catalysis of Light Nuclei Fusion in a Ball Lightning a Reality

    E-Print Network [OSTI]

    E. M. Drobyshevski

    2004-06-19

    In 1997, we proposed a model of the ball lightning (BL) whose activity is accounted for by energy release in the fusion of light nuclei, most probably, carbon in organic fibers (Proc. ISBL 97, p.157). The fusion is provided by catalytic action of superheavy negative particles making up the Galactic Dark Matter. We called them DArk Electric Matter Objects, or daemons. The daemons are assumed to be elementary black holes (M ~ 10^-5 g) carrying a charge of up to Ze = 10e. Experiments have culminated in 2000 by the discovery of daemons. We used the two-screen scintillation technique with a scintillator ZnS(Ag). Measurements showed the daemon flux striking the Earth to be ~10^-9 cm^-2s^-1 for an object velocity of as low as ~<10-30 km/s. The half-year periodicity of the flux was revealed, which can be assigned to daemons being captured into helio- and geocentric orbits as the Solar system moves through the DM background (see astro-ph/0402367). The next step in creating a daemon-mediated BL to achieve controlled energy generation through catalytic fusion of carbon nuclei will be a search for methods of capture and stable holding of a daemon, which could involve the use of the tip of a carbon fiber, as it apparently occurs in nature.

  11. Discovery of Daemons Makes Power Generation by Daemon-Assisted Catalysis of Light Nuclei Fusion in a Ball Lightning a Reality

    E-Print Network [OSTI]

    Drobyshevski, E M

    2004-01-01

    In 1997, we proposed a model of the ball lightning (BL) whose activity is accounted for by energy release in the fusion of light nuclei, most probably, carbon in organic fibers (Proc. ISBL 97, p.157). The fusion is provided by catalytic action of superheavy negative particles making up the Galactic Dark Matter. We called them DArk Electric Matter Objects, or daemons. The daemons are assumed to be elementary black holes (M ~ 10^-5 g) carrying a charge of up to Ze = 10e. Experiments have culminated in 2000 by the discovery of daemons. We used the two-screen scintillation technique with a scintillator ZnS(Ag). Measurements showed the daemon flux striking the Earth to be ~10^-9 cm^-2s^-1 for an object velocity of as low as ~<10-30 km/s. The half-year periodicity of the flux was revealed, which can be assigned to daemons being captured into helio- and geocentric orbits as the Solar system moves through the DM background (see astro-ph/0402367). The next step in creating a daemon-mediated BL to achieve controlled...

  12. Allostery through protein-induced DNA bubbles

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Traverso, Joseph J.; Manoranjan, Valipuram S.; Bishop, A. R.; Rasmussen, Kim Ø.; Voulgarakis, Nikolaos K.

    2015-03-12

    Allostery through DNA is increasingly recognized as an important modulator of DNA functions. Here, we show that the coalescence of protein-induced DNA bubbles can mediate allosteric interactions that drive protein aggregation. We propose that such allostery may regulate DNA's flexibility and the assembly of the transcription machinery. Mitochondrial transcription factor A (TFAM), a dual-function protein involved in mitochondrial DNA (mtDNA) packaging and transcription initiation, is an ideal candidate to test such a hypothesis owing to its ability to locally unwind the double helix. Numerical simulations demonstrate that the coalescence of TFAM-induced bubbles can explain experimentally observed TFAM oligomerization. The resultingmore »melted DNA segment, approximately 10 base pairs long, around the joints of the oligomers act as flexible hinges, which explains the efficiency of TFAM in compacting DNA. Since mitochondrial polymerase (mitoRNAP) is involved in melting the transcription bubble, TFAM may use the same allosteric interaction to both recruit mitoRNAP and initiate transcription.« less

  13. DNA Nanomechanical Switches under Folding Kinetics Control

    E-Print Network [OSTI]

    Meller, Amit

    DNA Nanomechanical Switches under Folding Kinetics Control Virgile Viasnoff,, Amit Meller operate at equilibrium under changes in solution composition. We propose an alternative DNA switch design after heat denaturation drives the switch to its lowest energy conformation, while rapid cooling (>100

  14. Dynamics and control of DNA sequence amplification

    SciTech Connect (OSTI)

    Marimuthu, Karthikeyan [Department of Chemical Engineering and Center for Advanced Process Decision-Making, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213 (United States); Chakrabarti, Raj, E-mail: raj@pmc-group.com, E-mail: rajc@andrew.cmu.edu [Department of Chemical Engineering and Center for Advanced Process Decision-Making, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213 (United States); Division of Fundamental Research, PMC Advanced Technology, Mount Laurel, New Jersey 08054 (United States)

    2014-10-28

    DNA amplification is the process of replication of a specified DNA sequence in vitro through time-dependent manipulation of its external environment. A theoretical framework for determination of the optimal dynamic operating conditions of DNA amplification reactions, for any specified amplification objective, is presented based on first-principles biophysical modeling and control theory. Amplification of DNA is formulated as a problem in control theory with optimal solutions that can differ considerably from strategies typically used in practice. Using the Polymerase Chain Reaction as an example, sequence-dependent biophysical models for DNA amplification are cast as control systems, wherein the dynamics of the reaction are controlled by a manipulated input variable. Using these control systems, we demonstrate that there exists an optimal temperature cycling strategy for geometric amplification of any DNA sequence and formulate optimal control problems that can be used to derive the optimal temperature profile. Strategies for the optimal synthesis of the DNA amplification control trajectory are proposed. Analogous methods can be used to formulate control problems for more advanced amplification objectives corresponding to the design of new types of DNA amplification reactions.

  15. Recombinant DNA encoding a desulfurization biocatalyst

    DOE Patents [OSTI]

    Rambosek, J.; Piddington, C.S.; Kovacevich, B.R.; Young, K.D.; Denome, S.A.

    1994-10-18

    This invention relates to a recombinant DNA molecule containing a gene or genes which encode a biocatalyst capable of desulfurizing a fossil fuel which contains organic sulfur molecules. For example, the present invention encompasses a recombinant DNA molecule containing a gene or genes of a strain of Rhodococcus rhodochrous. 13 figs.

  16. Prospects & Overviews Integrating DNA barcode data and

    E-Print Network [OSTI]

    DeSalle, Rob

    , and description Paul Z. Goldstein and Rob DeSalleà DNA barcodes, like traditional sources of taxonomic information interpretation. The role of DNA barcoding in generating hypotheses of new taxa in need of formal taxonomic information but also for our comprehension of the magnitude of species diversity and its disappearance

  17. The shape of the DNA minor groove directs binding by the DNA-bending protein Fis

    SciTech Connect (OSTI)

    Stella, Stefano; Cascio, Duilio; Johnson, Reid C.

    2010-06-21

    The bacterial nucleoid-associated protein Fis regulates diverse reactions by bending DNA and through DNA-dependent interactions with other control proteins and enzymes. In addition to dynamic nonspecific binding to DNA, Fis forms stable complexes with DNA segments that share little sequence conservation. Here we report the first crystal structures of Fis bound to high- and low-affinity 27-base-pair DNA sites. These 11 structures reveal that Fis selects targets primarily through indirect recognition mechanisms involving the shape of the minor groove and sequence-dependent induced fits over adjacent major groove interfaces. The DNA shows an overall curvature of {approx}65{sup o}, and the unprecedented close spacing between helix-turn-helix motifs present in the apodimer is accommodated by severe compression of the central minor groove. In silico DNA structure models show that only the roll, twist, and slide parameters are sufficient to reproduce the changes in minor groove widths and recreate the curved Fis-bound DNA structure. Models based on naked DNA structures suggest that Fis initially selects DNA targets with intrinsically narrow minor grooves using the separation between helix-turn-helix motifs in the Fis dimer as a ruler. Then Fis further compresses the minor groove and bends the DNA to generate the bound structure.

  18. Elastic and Proton Dynamics of the DNA

    E-Print Network [OSTI]

    V. L. Golo

    2008-03-28

    The subject of this report is the dynamics of elastic system in conjunction with hydrogen bonds of the DNA. We draw attention to the draw-back of the familiar rod model of the DNA, and make a case of constructing models that could accommodate the intrinsic structure of the DNA. In this respect studying the interplay among the elastic system and the protons of the DNA, is of interest, for it could accommodate the inter-strand as well as the tunneling modes of protons. Following this direction, we come to the conclusion that the elastic-proton dynamics may have a bearing on biophysics of the DNA. The phenomenon of point mutations is discussed within this framework.

  19. Programmable DNA-mediated multitasking processor

    E-Print Network [OSTI]

    Shu, Jian-Jun; Yong, Kian-Yan; Shao, Fangwei; Lee, Kee Jin

    2015-01-01

    Because of DNA appealing features as perfect material, including minuscule size, defined structural repeat and rigidity, programmable DNA-mediated processing is a promising computing paradigm, which employs DNAs as information storing and processing substrates to tackle the computational problems. The massive parallelism of DNA hybridization exhibits transcendent potential to improve multitasking capabilities and yield a tremendous speed-up over the conventional electronic processors with stepwise signal cascade. As an example of multitasking capability, we present an in vitro programmable DNA-mediated optimal route planning processor as a functional unit embedded in contemporary navigation systems. The novel programmable DNA-mediated processor has several advantages over the existing silicon-mediated methods, such as conducting massive data storage and simultaneous processing via much fewer materials than conventional silicon devices.

  20. Method for sequencing DNA base pairs

    DOE Patents [OSTI]

    Sessler, A.M.; Dawson, J.

    1993-12-14

    The base pairs of a DNA structure are sequenced with the use of a scanning tunneling microscope (STM). The DNA structure is scanned by the STM probe tip, and, as it is being scanned, the DNA structure is separately subjected to a sequence of infrared radiation from four different sources, each source being selected to preferentially excite one of the four different bases in the DNA structure. Each particular base being scanned is subjected to such sequence of infrared radiation from the four different sources as that particular base is being scanned. The DNA structure as a whole is separately imaged for each subjection thereof to radiation from one only of each source. 6 figures.

  1. Storing data encoded DNA in living organisms

    DOE Patents [OSTI]

    Wong; Pak C. (Richland, WA), Wong; Kwong K. (Sugar Land, TX), Foote; Harlan P. (Richland, WA)

    2006-06-06

    Current technologies allow the generation of artificial DNA molecules and/or the ability to alter the DNA sequences of existing DNA molecules. With a careful coding scheme and arrangement, it is possible to encode important information as an artificial DNA strand and store it in a living host safely and permanently. This inventive technology can be used to identify origins and protect R&D investments. It can also be used in environmental research to track generations of organisms and observe the ecological impact of pollutants. Today, there are microorganisms that can survive under extreme conditions. As well, it is advantageous to consider multicellular organisms as hosts for stored information. These living organisms can provide as memory housing and protection for stored data or information. The present invention provides well for data storage in a living organism wherein at least one DNA sequence is encoded to represent data and incorporated into a living organism.

  2. Particulate Carrier Systems for Mucosal DNA Vaccine Delivery

    E-Print Network [OSTI]

    Borchard, Gerrit

    2006-10-26

    Streptomyces griseus Stop solution: 1M KOH In humans: degradation by lysozyme Incubation with chitosanase (1) GPEN 2006 Free DNA chitoplexes Incubation with chitosanase, 37?C Intact DNA ? Degraded chitosan Intact DNA ? Extraction with phenol: chloroform... Streptomyces griseus Stop solution: 1M KOH In humans: degradation by lysozyme Incubation with chitosanase (1) GPEN 2006 Free DNA chitoplexes Incubation with chitosanase, 37?C Intact DNA ? Degraded chitosan Intact DNA ? Extraction with phenol: chloroform...

  3. Microfluidics: Kinetics of Hybridized DNA With Fluid Flow Variations...

    Office of Scientific and Technical Information (OSTI)

    Conference: Microfluidics: Kinetics of Hybridized DNA With Fluid Flow Variations. Citation Details In-Document Search Title: Microfluidics: Kinetics of Hybridized DNA With Fluid...

  4. Protein Bridges DNA Base and Nucleotide Excision Repair Pathways

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Bridges DNA Base and Nucleotide Excision Repair Pathways Print Alkyltransferase proteins (AGT) protect cells from the biological effects of DNA damage caused by the addition...

  5. IN VITRO MUTAGENIC AND DNA AND CHROMOSOMAL DAMAGE ACTIVITY BY...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    IN VITRO MUTAGENIC AND DNA AND CHROMOSOMAL DAMAGE ACTIVITY BY SURFACTANT DISPERSION OR SOLVENT EXTRACT OF A REFERENCE DIESEL EXHAUST PARTICULATE MATERIAL IN VITRO MUTAGENIC AND DNA...

  6. Topoisomerase II Structure Suggests Novel DNA Cleavage Mechanism

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Topoisomerase II Structure Suggests Novel DNA Cleavage Mechanism Print Type II topoisomerases are molecular machines that regulate DNA supercoiling and separate interlocked...

  7. Gel Electrophoresis of Gold-DNA Nanoconjugates

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Pellegrino, T.; Sperling, R. A.; Alivisatos, A. P.; Parak, W. J.

    2007-01-01

    Gold-DNA conjugates were investigated in detail by a comprehensive gel electrophoresis study based on 1200 gels. A controlled number of single-stranded DNA of different length was attached specifically via thiol-Au bonds to phosphine-stabilized colloidal gold nanoparticles. Alternatively, the surface of the gold particles was saturated with single stranded DNA of different length either specifically via thiol-Au bonds or by nonspecific adsorption. From the experimentally determined electrophoretic mobilities, estimates for the effective diameters of the gold-DNA conjugates were derived by applying two different data treatment approaches. The first method is based on making a calibration curve for the relation between effectivemore »diameters and mobilities with gold nanoparticles of known diameter. The second method is based on Ferguson analysis which uses gold nanoparticles of known diameter as reference database. Our study shows that effective diameters derived from gel electrophoresis measurements are affected with a high error bar as the determined values strongly depend on the method of evaluation, though relative changes in size upon binding of molecules can be detected with high precision. Furthermore, in this study, the specific attachment of DNA via gold-thiol bonds to Au nanoparticles is compared to nonspecific adsorption of DNA. Also, the maximum number of DNA molecules that can be bound per particle was determined.« less

  8. Method of quantitating dsDNA

    DOE Patents [OSTI]

    Stark, Peter C. (Los Alamos, NM); Kuske, Cheryl R. (Los Alamos, NM); Mullen, Kenneth I. (Los Alamos, NM)

    2002-01-01

    A method for quantitating dsDNA in an aqueous sample solution containing an unknown amount of dsDNA. A first aqueous test solution containing a known amount of a fluorescent dye-dsDNA complex and at least one fluorescence-attenutating contaminant is prepared. The fluorescence intensity of the test solution is measured. The first test solution is diluted by a known amount to provide a second test solution having a known concentration of dsDNA. The fluorescence intensity of the second test solution is measured. Additional diluted test solutions are similarly prepared until a sufficiently dilute test solution having a known amount of dsDNA is prepared that has a fluorescence intensity that is not attenuated upon further dilution. The value of the maximum absorbance of this solution between 200-900 nanometers (nm), referred to herein as the threshold absorbance, is measured. A sample solution having an unknown amount of dsDNA and an absorbance identical to that of the sufficiently dilute test solution at the same chosen wavelength is prepared. Dye is then added to the sample solution to form the fluorescent dye-dsDNA-complex, after which the fluorescence intensity of the sample solution is measured and the quantity of dsDNA in the sample solution is determined. Once the threshold absorbance of a sample solution obtained from a particular environment has been determined, any similarly prepared sample solution taken from a similar environment and having the same value for the threshold absorbance can be quantified for dsDNA by adding a large excess of dye to the sample solution and measuring its fluorescence intensity.

  9. Nanopores formed by DNA origami: a review

    E-Print Network [OSTI]

    Bell, Nicholas A. W.; Keyser, Ulrich F.

    2014-06-10

    , coated with hydrophobic moieties, into a lipid bilayer. Recent work in these two branches is now discussed. Hybrid nanopores formed by trapping DNA origami onto a solid state nanopore The combination of DNA origami and solid state nanopores was first... (1982) Nucleic acid junctions and lattices. J. Theor. Biol. 99, 237–47. 28 Rothemund PWK (2006) Folding DNA to create nanoscale shapes and patterns. Nature 440, 297– 302. 29 Kuzyk A, Schreiber R, Fan Z, Pardatscher G, Roller E-M, Högele A, Simmel FC...

  10. DockingShop: A Tool for Interactive Molecular Docking (Conference) |

    Office of Scientific and Technical Information (OSTI)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of NaturalDukeWakefieldSulfate Reducing(Journal Article) | SciTech Connect Discrimination of newTransportgrowth

  11. DockingShop: A Tool for Interactive Molecular Docking (Conference) |

    Office of Scientific and Technical Information (OSTI)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of NaturalDukeWakefieldSulfate Reducing(Journal Article) | SciTech Connect Discrimination of newTransportgrowthSciTech

  12. DNA Assembly Line for Nano-Construction

    ScienceCinema (OSTI)

    Oleg Gang

    2010-01-08

    Building on the idea of using DNA to link up nanoparticles scientists at Brookhaven National Lab have designed a molecular assembly line for high-precision nano-construction. Nanofabrication is essential for exploiting the unique properties of nanoparticl

  13. Casting inorganic structures with DNA molds

    E-Print Network [OSTI]

    Sun, Wei

    We report a general strategy for designing and synthesizing inorganic nanostructures with arbitrarily prescribed three-dimensional shapes. Computationally designed DNA strands self-assemble into a stiff “nanomold” that ...

  14. Deoxyribose oxidation chemistry and endogenous DNA adducts

    E-Print Network [OSTI]

    Zhou, Xinfeng

    2006-01-01

    Endogenous and exogenous oxidants react with cellular macromolecules to generate a variety of electrophiles that react with DNA produce cytotoxic and mutagenic adducts. One source of such electrophiles is deoxyribose in ...

  15. Ubiquitylation, neddylation and the DNA damage response

    E-Print Network [OSTI]

    Brown, Jessica S.; Jackson, Stephen P.

    2015-04-01

    , collectively termed the DNA damage response (DDR), requires the recruitment and subsequent post-translational modification (PTM) of a complex network of proteins. Ubiquitin and the ubiquitin-like protein (UBL) SUMO have established roles in regulating...

  16. NIJ-Funded Research in Forensic DNA

    E-Print Network [OSTI]

    Standards Funding from the FBI S&T Branch through NIST Information Access Division httpDNA) · Technology Evaluation and Development ­ Rapid multiplex PCR protocols* (PCR: 3 hr to FBI

  17. Intriguing DNA Editor Has a Structural Trigger

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    "plug in" very specific snippets of DNA coding for a variety of reasons. For example, microbes could be re-engineered to consume specific environmental toxins or to produce better...

  18. Linear Thermodynamics of Rodlike DNA Filtration

    E-Print Network [OSTI]

    Li, Zirui

    Linear thermodynamics transportation theory is employed to study filtration of rodlike DNA molecules. Using the repeated nanoarray consisting of alternate deep and shallow regions, it is demonstrated that the complex ...

  19. Extracting biological knowledge from DNA sequences

    SciTech Connect (OSTI)

    De La Vega, F.M.; Thieffry, D.; Collado-Vides, J.

    1996-12-31

    This session describes the elucidation of information from dna sequences and what challenges computational biologists face in their task of summarizing and deciphering the human genome. Techniques discussed include methods from statistics, information theory, artificial intelligence and linguistics. 1 ref.

  20. Euler buckling and nonlinear kinking of double-stranded DNA

    E-Print Network [OSTI]

    Cohen, Adam E.

    physiological conditions: at high curvature, does the DNA bend smoothly, or does it kink like a drinking straw and damage). These aspects of DNA mechanics are likely to influence protein binding and DNA packaging, yet the effect of single-nucleotide mismatches on DNA bending. Our data support a model of linear elastic bending

  1. The unholy trinity: taxonomy, species delimitation and DNA barcoding

    E-Print Network [OSTI]

    DeSalle, Rob

    The unholy trinity: taxonomy, species delimitation and DNA barcoding Rob DeSalle*, Mary G. Egan are clarified and resolved, before the use of DNA as a tool for taxonomy and species delimitation can framework for interweaving classical taxonomy with the goals of `DNA barcoding'. Keywords: DNA barcoding

  2. Synopsis: Repulsion Helps Virus Pack DNA APS/Joan Tycko

    E-Print Network [OSTI]

    Smith, Douglas E.

    Synopsis: Repulsion Helps Virus Pack DNA APS/Joan Tycko Repulsive DNA-DNA Interactions Accelerate, and Douglas E. Smith Phys. Rev. Lett. 112, 248101 (2014) Published June 17, 2014 Featured in Physics Editors reported in Physical Review Letters show that, surprisingly, switching the DNA self-interaction from

  3. DNA Concentration By UV Spectrophotometry Measure Absorption

    E-Print Network [OSTI]

    Aris, John P.

    DNA Concentration By UV Spectrophotometry Measure Absorption: 1. Dilute DNA to 0.5 to 50 µg/ml in TE buffer or dH2O. Plan to use a quartz cuvette or a UV-transparent plastic (disposable) cuvette (1 2. Measure absorption at 260 nm (A260). Start by zeroing instrument with TE buffer or dH2O alone

  4. Overview of DNA Programs at NIST

    E-Print Network [OSTI]

    .nist.gov/mml/bmd/genetics/applied_genetics_pubs.cfm Pete Vallone Erica Butts DNA Biometrics Team Funding from the FBI S&T Branch through NIST Information http://www.cstl.nist.gov/strbase/NISTpub.htm +FBI-funding (DNA biometrics) Workshops 0 0 0 1 2 7 9 6 11) measurement calibration Required under FBI Quality Assurance Standard 9.5.5 for labs connected to the national

  5. Carbon Nanotube DNA Sensor and Sensing Mechanism

    E-Print Network [OSTI]

    Le Roy, Robert J.

    nanotube (SWNT) DNA sensors and the sensing mechanism. The simple and generic protocol for label for direct label-free detection of DNA hybridization in a biocompatible buffer solution. We also carried out is a field effect device, which has a typical on-current of 3-6 µA at 10 mV source- drain bias and an on-off

  6. Denaturation of DNA at high salt concentrations

    E-Print Network [OSTI]

    Maity, Arghya; Singh, Navin

    2015-01-01

    Cations present in the solution are important for the stability of two negative strands of DNA molecules. Experimental as well as theoretical results show that the DNA molecule is more stable as the concentration of salt (or cations) increases. It is known that the two strands of DNA molecule carry negative charge due to phosphate group along the strands. These cations act as a shielding particles to the two like charge strands. Recently, in an experiment it is shown that there is a critical value in the concentration of salts (or cations) that can stabilize the helical structure of DNA. If one add more salt in the solution beyond this critical value, the stability of the DNA molecule will disrupt. In this work we study the stability of DNA molecules at higher concentrations. How the stability at higher concentration can be explained through some theoretical calculations is the aim of this manuscript. We consider the PBD model with proper modifications that can explain the negative stability of the molecule a...

  7. Denaturation of DNA at high salt concentrations

    E-Print Network [OSTI]

    Arghya Maity; Amar Singh; Navin Singh

    2015-08-19

    Cations present in the solution are important for the stability of two negative strands of DNA molecules. Experimental as well as theoretical results show that the DNA molecule is more stable as the concentration of salt (or cations) increases. It is known that the two strands of DNA molecule carry negative charge due to phosphate group along the strands. These cations act as a shielding particles to the two like charge strands. Recently, in an experiment it is shown that there is a critical value in the concentration of salts (or cations) that can stabilize the helical structure of DNA. If one add more salt in the solution beyond this critical value, the stability of the DNA molecule will disrupt. In this work we study the stability of DNA molecules at higher concentrations. How the stability at higher concentration can be explained through some theoretical calculations is the aim of this manuscript. We consider the PBD model with proper modifications that can explain the negative stability of the molecule at higher concentration. Our findings are in close match with the experimental results.

  8. DNA nanotechnology: understanding and optimisation through simulation

    E-Print Network [OSTI]

    Thomas E. Ouldridge

    2014-11-07

    DNA nanotechnology promises to provide controllable self-assembly on the nanoscale, allowing for the design of static structures, dynamic machines and computational architectures. In this article I review the state-of-the art of DNA nanotechnology, highlighting the need for a more detailed understanding of the key processes, both in terms of theoretical modelling and experimental characterisation. I then consider coarse-grained models of DNA, mesoscale descriptions that have the potential to provide great insight into the operation of DNA nanotechnology if they are well designed. In particular, I discuss a number of nanotechnological systems that have been studied with oxDNA, a recently developed coarse-grained model, highlighting the subtle interplay of kinetic, thermodynamic and mechanical factors that can determine behaviour. Finally, new results highlighting the importance of mechanical tension in the operation of a two-footed walker are presented, demonstrating that recovery from an unintended `overstepped' configuration can be accelerated by three to four orders of magnitude by application of a moderate tension to the walker's track. More generally, the walker illustrates the possibility of biasing strand-displacement processes to affect the overall rate.

  9. Conditions for positioning of nucleosomes on DNA

    E-Print Network [OSTI]

    Michael Sheinman; Ho-Ryun Chung

    2015-04-29

    Positioning of nucleosomes along eukaryotic genomes plays an important role in their organization and regulation. There are many different factors affecting the location of nucleosomes. Some can be viewed as preferential binding of a single nucleosome to different locations along the DNA and some as interactions between neighboring nucleosomes. In this study we analyzed how well nucleosomes are positioned along the DNA as a function of strength of the preferential binding, correlation length of the binding energy landscape, interactions between neighboring nucleosomes and others relevant system properties. We analyze different scenarios: designed energy landscapes and generically disordered ones and derive conditions for good positioning. Using analytic and numerical approaches we find that, even if the binding preferences are very weak, synergistic interplay between the interactions and the binding preferences is essential for a good positioning of nucleosomes, especially on correlated energy landscapes. Analyzing empirical energy landscape, we discuss relevance of our theoretical results to positioning of nucleosomes on DNA \\emph{in vivo.}

  10. Flow cytometric measurement of total DNA and incorporated halodeoxyuridine

    DOE Patents [OSTI]

    Dolbeare, Frank A. (Livermore, CA); Gray, Joe W. (Livermore, CA)

    1986-01-01

    A method for the simultaneous flow cytometric measurement of the total DNA content and the level of DNA synthesis in normal and malignant cells is disclosed. The sensitivity of the method allows a study of cell cycle traverse rates for large scale cell populations as well as single cell measurements. A DNA stain such as propidium iodide is used as the probe for the measurement of total DNA content and a monoclonal antibody reactive with a DNA precursor such as bromodeoxyuridine (BrdU) is used as a probe for the measurement of BrdU uptake by the cells as a measure of DNA synthesis.

  11. DNA analysis conference in Santa Fe

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home Room NewsInformation Current HAB Packet Hanford Advisory Board6/23/2014DLFM libraryDNA OrigamiDNA

  12. Analysis of the structural changes caused by positive DNA supercoiling

    E-Print Network [OSTI]

    Barth, Marita Christine

    2007-01-01

    The procession of helix-tracking enzymes along a DNA molecule results in the formation of supercoils in the DNA, with positive supercoiling (overwinding) generated ahead of the enzyme, and negative supercoiling (underwinding) ...

  13. Engineering of DNA-mediated assemblies for biosensing applications /

    E-Print Network [OSTI]

    Xu, Phyllis F.

    2013-01-01

    OF THE DISSERTATION Engineering of DNA-mediated assembliesCALIFORNIA, SAN DIEGO Engineering of DNA-mediated assembliesMaterials Science and Engineering by Phyllis F. Xu Committee

  14. Manipulation of cellular DNA repair by early adenovirus proteins

    E-Print Network [OSTI]

    Orazio, Nicole Ise

    2010-01-01

    The function of BLM in the DDR and in processing of DNA endscellular DNA damage response (DDR) functions to inhibit thespecific components of the DDR, and provides insight into

  15. DNA hybridization : fundamental studies and applications in directed assembly

    E-Print Network [OSTI]

    Bajaj, Manish G. (Manish Gopal)

    2005-01-01

    Programmed self-assembly using non-covalent DNA-DNA interactions is a promising technique for the creation of next-generation functional devices for electronic, optical, and magnetic applications. This thesis develops the ...

  16. Genome scanning : an AFM-based DNA sequencing technique

    E-Print Network [OSTI]

    Elmouelhi, Ahmed (Ahmed M.), 1979-

    2003-01-01

    Genome Scanning is a powerful new technique for DNA sequencing. The method presented in this thesis uses an atomic force microscope with a functionalized cantilever tip to sequence single stranded DNA immobilized to a mica ...

  17. Defining functional DNA elements in the human genome

    E-Print Network [OSTI]

    Kellis, Manolis

    With the completion of the human genome sequence, attention turned to identifying and annotating its functional DNA elements. As a complement to genetic and comparative genomics approaches, the Encyclopedia of DNA Elements ...

  18. THE NEW YORK BOTANICAL GARDEN DNA BANK ACQUISITIONS POLICY

    E-Print Network [OSTI]

    Law, Wayne

    THE NEW YORK BOTANICAL GARDEN DNA BANK ACQUISITIONS POLICY The DNA Bank of The New York Botanical herbarium. Please contact Lisa M. Campbell, Plant Research Laboratory, The New York Botanical Garden, Bronx

  19. Unveiling Stability Criteria of DNA-Carbon Nanotubes Constructs...

    Office of Scientific and Technical Information (OSTI)

    constructs. Application of STM allows direct observation of very stable CNT-DNA hybrid structures with the well-defined DNA wrapping angle of 63.4and a coiling period of...

  20. Dna electrophoresis in photopolymerized polyacrylamide gels on a microfluidic device 

    E-Print Network [OSTI]

    Lo, Chih-Cheng

    2009-05-15

    -throughput DNA gel electrophoresis. However, further progress toward dramatic improvements of separation performance over ultra-short distances requires a much more detailed understanding of the physics of DNA migration in the sieving gel matrix than is currently...

  1. Enhancement of in vitro Translation by Gold Nanoparticle – DNA Conjugates

    E-Print Network [OSTI]

    Park, Sunho

    Gold nanoparticle (AuNP)?DNA conjugates can enhance in vitro translation of a protein. Enhancement occurs via a combination of nonspecific adsorption of translation-related molecules and the ribosome to the AuNP?DNA and ...

  2. Role of DNA repair protein ERCC1 in skin cancer 

    E-Print Network [OSTI]

    Song, Liang

    2009-01-01

    Nucleotide excision repair (NER) is one of the major repair systems for removal of DNA lesions. The NER pathway has evolved mainly to repair UV-induced DNA damage and is also active against a broad range of endogenously ...

  3. A model for sample stacking in microcapillary DNA electrophoresis

    E-Print Network [OSTI]

    Srivastava, Alok Kumar, 1967-

    2002-01-01

    Sanger's method of chain termination is the method of choice in DNA sequencing, where electrophoresis is used to separate the different sized DNA. In the past decade, microfabricated capillary devices have been developed ...

  4. Flow cytometric measurement of total DNA and incorporated halodeoxyuridine

    DOE Patents [OSTI]

    Dolbeare, F.A.; Gray, J.W.

    1983-10-18

    A method for the simultaneous flow cylometric measurement of total cellular DNA content and of the uptake of DNA precursors as a measure of DNA synthesis during various phases of the cell cycle in normal and malignant cells in vitro and in vivo is described. The method comprises reacting cells with labelled halodeoxyuridine (HdU), partially denaturing cellular DNA, adding to the reaction medium monoclonal antibodies (mabs) reactive with HdU, reacting the bound mabs with a second labelled antibody, incubating the mixture with a DNA stain, and measuring simultaneously the intensity of the DNA stain as a measure of the total cellular DNA and the HdU incorporated as a measure of DNA synthesis. (ACR)

  5. Photoelectrochemical array platform for genomic scale DNA synthesis

    E-Print Network [OSTI]

    Emig, Christopher Joseph

    2006-01-01

    Molecular and synthetic biologists have increasing demand for large, high-fidelity constructs of synthetic DNA. Recent developments in harvesting oligonucleotides from DNA microarrays has proven that these can be assembled ...

  6. Single cell trapping and DNA damage analysis using microwell arrays

    E-Print Network [OSTI]

    Wood, David

    With a direct link to cancer, aging, and heritable diseases as well as a critical role in cancer treatment, the importance of DNA damage is well-established. The intense interest in DNA damage in applications ranging from ...

  7. Mitigating security issues in the evolving DNA synthesis industry

    E-Print Network [OSTI]

    Turlington, Ralph Donald, III

    2013-01-01

    DNA synthesis technologies are advancing at exponential rates, with production of ever longer, more complex, and less expensive sequences of double stranded DNA. This has fostered development of industrial scale design, ...

  8. Alternative Methods for Human Identification: Mitochondrial DNA Base Composition Profiling

    E-Print Network [OSTI]

    Applied Genetics Alternative Methods for Human Identification: Mitochondrial DNA Base Composition · Evaluation Experiments · Future directions #12;Applied Genetics Mitochondrial DNA · Mitochondria are organelles within cells ­ Produce energy via the Krebs Cycle · Separate genome from the nucleus ­ 16,569 bp

  9. The Genetic Structure of the Kuwaiti Population: Mitochondrial DNA Markers

    E-Print Network [OSTI]

    Theyab, Jasem

    2010-06-14

    the expansion of early Homo sapiens out of Africa. Kuwait is located in the Northeast portion of the Arabian Peninsula. This thesis investigated the mitochondrial DNA (mtDNA) genetic variation in 117 unrelated individuals to determine the genetic structure...

  10. Lectin cDNA and transgenic plants derived therefrom

    DOE Patents [OSTI]

    Raikhel, Natasha V. (Okemos, MI)

    2000-10-03

    Transgenic plants containing cDNA encoding Gramineae lectin are described. The plants preferably contain cDNA coding for barley lectin and store the lectin in the leaves. The transgenic plants, particularly the leaves exhibit insecticidal and fungicidal properties.

  11. International Standards in Forensic DNA and

    E-Print Network [OSTI]

    documentary (technical) standards physical (measurement) standards Certified reference material to aidInternational Standards in Forensic DNA and Recent Forensic Science Activities in the United States John M. Butler, Ph.D. National Institute of Standards and Technology NIST Fellow & Special Assistant

  12. Isolation of Yeast DNA Prepare in advance

    E-Print Network [OSTI]

    Aris, John P.

    Isolation of Yeast DNA Prepare in advance: · 2.0 ml microfuge tubes containing 0.25 g of 0.5 mm600 ~1, or 1 ml saturated YPD culture at OD600 ~10). Centrifuge for 5 minutes at 2000 rpm. Discard will thaw during centrifugation. Transfer supernatant (200-225 µl) to a fresh 1.5 ml tube. Avoid

  13. Rewritable Memory by Controllable Nanopatterning of DNA

    E-Print Network [OSTI]

    Pierce, Niles A.

    ABSTRACT Fabricating a nanostructure capable of reversibly patterning molecules is a fundamental goal within nanotechnology, underlying diverse processes such as information storage, scaffold functioning of the device as rewritable memory. The bit state of each address is controlled by specific DNA

  14. Research Article DNA sequencing by microchip

    E-Print Network [OSTI]

    Barron, Annelise E.

    .1002/elps.200800389 1 Introduction The completion of the Human Genome Project [1, 2] has led to tremendous of the reasons for the early completion of the Human Genome Project was the technological advance- ment in DNA of personalized medicine based on the human genome cannot be fully realized until the cost of full human genome

  15. Advanced Review Mediators and dynamics of DNA

    E-Print Network [OSTI]

    Wang, Wei

    -wide classifies cell types uniquely and in several cases discriminates between healthy and cancerous cell types zinc fin- ger proteins, recognize the presence of methylated cytosines.4­13 DNMT1 maintains methylation mechanism that prevents the activation of these sequences.31­34 DNA methyla- tion is also present in genes

  16. Identification of animal images based on DNA

    E-Print Network [OSTI]

    Wolf, Lior

    ongus ? Given mtDNA, the algorithm identifies the correct unseen image #12;Data sets Fishes of Australia Regularized CCA #12;Results · 93 fish species, 82 for training, 11 for testing · Significantly better than chance or NN #12;Results Fish: 90% correct Birds: 72% correct Dorsal: 59% correct Head: 56% correct

  17. Conserved Steps in Eukaryotic DNA Replication

    E-Print Network [OSTI]

    Blow, J. Julian

    and 50 car- bons of deoxyribose. The 10 carbon of the deoxyribose is linked to one of four different of DNA contains all the information necessary to produce new second strands through complementary base the input of chemical energy to break the hydrogen bonds. This energy is derived from hydrolysis of ATP

  18. A User's Guide to the Encyclopedia of DNA Elements (ENCODE)

    E-Print Network [OSTI]

    Bernstein, Bradley E.

    The mission of the Encyclopedia of DNA Elements (ENCODE) Project is to enable the scientific and medical communities to

  19. Structural basis for the inhibition of human alkyladenine DNA by 3,N4-ethenocytosine containing DNA

    E-Print Network [OSTI]

    Lingaraju, Gondichatnahalli M.

    Reactive oxygen and nitrogen species, generated by neutrophils and macrophages in chronically inflamed tissues, readily damage DNA, producing a variety of potentially genotoxic etheno base lesions; such inflammation-related ...

  20. Validation of DNA probes for molecular cytogenetics by mapping onto immobilized circular DNA

    E-Print Network [OSTI]

    Greulich-Bode, Karin

    2009-01-01

    two linked P1 clones (‘3012’, ‘3015’) that contain one orcircular DNA molecule (‘3012’) as well as the RGB image (J)overlap between clones ‘3012’ and ‘3015’ and the location of

  1. RhoJ Regulates Melanoma Chemoresistance by Suppressing Pathways that Sense DNA Damage

    E-Print Network [OSTI]

    Ho, Hsiang; Aruri, Jayavani; Kapadia, Rubina; Mehr, Hootan; White, Michael A.; Ganesan, Anand K.

    2012-01-01

    Pathways that Sense DNA Damage $watermark-text Hsiang Ho 1 ,16. Roos WP, Kaina B. DNA damage-induced apoptosis: FromDNA lesions to the DNA damage response and apoptosis. Cancer

  2. An Investigation on Gel Electrophoresis with Quantum Dots End-labeled DNA 

    E-Print Network [OSTI]

    Chen, Xiaojia

    2009-05-15

    explored manipulating DNA fragments by end labeling DNA molecules with quantum dot nanocrystals. The quantum dot-DNA conjugates can be further modified through binding interactions with biotinylated single-stranded DNA primers. Single molecule visualization...

  3. Protein search for multiple targets on DNA

    E-Print Network [OSTI]

    Martin Lange; Maria Kochugaeva; Anatoly B. Kolomeisky

    2015-08-03

    Protein-DNA interactions are crucial for all biological processes. One of the most important fundamental aspects of these interactions is the process of protein searching and recognizing specific binding sites on DNA. A large number of experimental and theoretical investigations have been devoted to uncovering the molecular description of these phenomena, but many aspects of the mechanisms of protein search for the targets on DNA remain not well understood. One of the most intriguing problems is the role of multiple targets in protein search dynamics. Using a recently developed theoretical framework we analyze this question in detail. Our method is based on a discrete-state stochastic approach that takes into account most relevant physical-chemical processes and leads to fully analytical description of all dynamic properties. Specifically, systems with two and three targets have been explicitly investigated. It is found that multiple targets in most cases accelerate the search in comparison with a single target situation. However, the acceleration is not always proportional to the number of targets. Surprisingly, there are even situations when it takes longer to find one of the multiple targets in comparison with the single target. It depends on the spatial position of the targets, distances between them, average scanning lengths of protein molecules on DNA, and the total DNA lengths. Physical-chemical explanations of observed results are presented. Our predictions are compared with experimental observations as well as with results from a continuum theory for the protein search. Extensive Monte Carlo computer simulations fully support our theoretical calculations.

  4. Generation of Full-Length cDNA Library

    E-Print Network [OSTI]

    Chuong, Cheng-Ming

    Generation of Full- Length cDNA Library from Single Human Prostate Cancer Cells BioTechniques 27 are performed on fixed and per- meabilized cells. Subsequent RT-PCR generates full-length cDNA libraries. Flowchart of current method for generating a full-length cDNA library from single cells. Cell fixation

  5. Mechanochemistry of a Viral DNA Packaging Motor , Jeffrey Moffitt1

    E-Print Network [OSTI]

    Oster, George

    Mechanochemistry of a Viral DNA Packaging Motor Jin Yu1 , Jeffrey Moffitt1 , Craig L. Hetherington1 The pentameric ATPase motor gp16 packages double-stranded DNA into the bacteriophage 29 virus capsid to explain how the packaging motor translocates the DNA in bursts of four 2.5 bp power strokes, while

  6. Recent advances in yeast molecular biology: recombinant DNA. [Lead abstract

    SciTech Connect (OSTI)

    Not Available

    1982-09-01

    Separate abstracts were prepared for the 25 papers presented at a workshop focusing on chromosomal structure, gene regulation, recombination, DNA repair, and cell type control, that have been obtained by experimental approaches incorporating the new technologies of yeast DNA transformation, molecular cloning, and DNA sequence analysis. (KRM)

  7. Footprinting proteinDNA complexes using the hydroxyl radical

    E-Print Network [OSTI]

    Tullius, Thomas D.

    Footprinting protein­DNA complexes using the hydroxyl radical Swapan S Jain & Thomas D Tullius.2008.72 Hydroxyl radical footprinting has been widely used for studying the structure of DNA and DNA­protein complexes. The high reactivity and lack of base specificity of the hydroxyl radical makes it an excellent

  8. Single-DNA Molecule Nanomotor Regulated by Photons

    E-Print Network [OSTI]

    Tan, Weihong

    Single-DNA Molecule Nanomotor Regulated by Photons Huaizhi Kang, Haipeng Liu, Joseph A. Phillips the design of a single-molecule nanomotor driven by photons. The nanomotor is a DNA hairpin photonic wires,8 enzyme assemblies,9 and functional DNA probes.10,11 Similar to RNA or protein

  9. Advances in DNA Testing for Genealogy and Anthropology

    E-Print Network [OSTI]

    Aazhang, Behnaam

    Advances in DNA Testing for Genealogy and Anthropology Join the MIT Enterprise Forum of Texas that are affecting Genealogy, Anthropology, Medicine, and more. In the second half of the program, Russ Capper company in the world for Genealogy (Family Tree DNA). Family Tree DNA has collaborated with other

  10. Can we model DNA at the mesoscale ? Comment on: Fluctuations in the DNA double helix: A critical review

    E-Print Network [OSTI]

    Peyrard, Michel

    2015-01-01

    Comment on "Fluctuations in the DNA double helix: A critical review" by Frank-Kamenetskii and Prakash

  11. DNA digestion protocol & hints Overview: Although it is pretty standard to digest DNA with restriction enzymes, here

    E-Print Network [OSTI]

    Doering, Tamara

    Liu 4/2004 DNA digestion protocol & hints Overview: Although it is pretty standard to digest DNA in molecular biology (3.1.1-3.1.2) Materials: · DNA sample in water or TE buffer · 10x digestion buffer.1 to 4 µg 10x Digestion buffer 2 µl 5 µl Enzyme ? ? Water Rest of volume Rest of volume 2. Add the enzyme

  12. Carrier DNA For Yeast Transformation Preparation of high molecular weight single stranded carrier DNA for yeast transformations.

    E-Print Network [OSTI]

    Aris, John P.

    minute pulses. Chill on ice for 1 minute in between. The DNA solution should not heat above room into ice bucket to cool quickly. Keep on ice. This denaturation step also sterilizes the DNA. 10. Freeze to collect solution at bottom of tube. Place on ice. 3. Sonicate DNA solution in tube with tip sonifier

  13. T4 DNA condensation in water-alcohol media

    E-Print Network [OSTI]

    M. O. Gallyamov; O. A. Pyshkina; V. G. Sergeyev; I. V. Yaminsky

    2011-07-21

    The process of compaction of high molecular weight DNA T4 is investigated directly in a AFM liquid cell. The AFM-images of globules formed by DNA molecules in the result of compaction in water-alcohol environments at high izopropanol concentration (80%) are received; it is found that at intermediate concentration of izopropanol (40-50%) the DNA molecules form partially compacted formations in which the separate coils of macromolecules twist in toroidal structures. It is shown using the technique of deconvolution of the AFM-images that the globule include only one closely packed DNA molecule. The model of DNA packing is proposed on the basis of AFM experiment.

  14. Preparation of DNA-containing extract for PCR amplification

    DOE Patents [OSTI]

    Dunbar, John M.; Kuske, Cheryl R.

    2006-07-11

    Environmental samples typically include impurities that interfere with PCR amplification and DNA quantitation. Samples of soil, river water, and aerosol were taken from the environment and added to an aqueous buffer (with or without detergent). Cells from the sample are lysed, releasing their DNA into the buffer. After removing insoluble cell components, the remaining soluble DNA-containing extract is treated with N-phenacylthiazolium bromide, which causes rapid precipitation of impurities. Centrifugation provides a supernatant that can be used or diluted for PCR amplification of DNA, or further purified. The method may provide a DNA-containing extract sufficiently pure for PCR amplification within 5–10 minutes.

  15. Ion Runaway in Lightning Discharges

    E-Print Network [OSTI]

    Landreman, Matt

    Runaway ions can be produced in plasmas with large electric fields, where the accelerating electric force is augmented by the low mean ionic charge due to the imbalance between the number of electrons and ions. Here we ...

  16. Inhibition of DNA ejection from bacteriophage by Mg+2 counterions

    E-Print Network [OSTI]

    SeIl Lee; C. V. Tran; T. T. Nguyen

    2010-11-09

    The problem of inhibiting viral DNA ejection from bacteriophages by multivalent counterions, specifically Mg$^{+2}$ counterions, is studied. Experimentally, it is known that MgSO$_4$ salt has a strong and non-monotonic effect on the amount of DNA ejected. There exists an optimal concentration at which the minimum amount of DNA is ejected from the virus. At lower or higher concentrations, more DNA is ejected from the capsid. We propose that this phenomenon is the result of DNA overcharging by Mg$^{+2}$ multivalent counterions. As Mg$^{+2}$ concentration increases from zero, the net charge of DNA changes from negative to positive. The optimal inhibition corresponds to the Mg$^{+2}$ concentration where DNA is neutral. At lower/higher concentrations, DNA genome is charged. It prefers to be in solution to lower its electrostatic self-energy, which consequently leads to an increase in DNA ejection. By fitting our theory to available experimental data, the strength of DNA$-$DNA short range attraction energies, mediated by Mg$^{+2}$, is found to be $-$0.004 $k_BT$ per nucleotide base. This and other fitted parameters agree well with known values from other experiments and computer simulations. The parameters are also in aggreement qualitatively with values for tri- and tetra-valent counterions.

  17. MCM ring hexamerization is a prerequisite for DNA-binding

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Froelich, Clifford A.; Nourse, Amanda; Enemark, Eric J.

    2015-09-13

    The hexameric Minichromosome Maintenance (MCM) protein complex forms a ring that unwinds DNA at the replication fork in eukaryotes and archaea. Our recent crystal structure of an archaeal MCM N-terminal domain bound to single-stranded DNA (ssDNA) revealed ssDNA associating across tight subunit interfaces but not at the loose interfaces, indicating that DNA-binding is governed not only by the DNA-binding residues of the subunits (MCM ssDNA-binding motif, MSSB) but also by the relative orientation of the subunits. We now extend these findings to show that DNA-binding by the MCM N-terminal domain of the archaeal organism Pyrococcus furiosus occurs specifically in themore »hexameric oligomeric form. We show that mutants defective for hexamerization are defective in binding ssDNA despite retaining all the residues observed to interact with ssDNA in the crystal structure. One mutation that exhibits severely defective hexamerization and ssDNA-binding is at a conserved phenylalanine that aligns with the mouse Mcm4(Chaos3) mutation associated with chromosomal instability, cancer, and decreased intersubunit association.« less

  18. Distinct kinetics of human DNA ligases I, IIIalpha, IIIbeta, and IV reveal direct DNA sensing ability and differential physiological functions in DNA repair

    SciTech Connect (OSTI)

    Chen, Xi; Ballin, Jeff D.; Della-Maria, Julie; Tsai, Miaw-Sheue; White, Elizabeth J.; Tomkinson, Alan E.; Wilson, Gerald M.

    2009-05-11

    The three human LIG genes encode polypeptides that catalyze phosphodiester bond formation during DNA replication, recombination and repair. While numerous studies have identified protein partners of the human DNA ligases (hLigs), there has been little characterization of the catalytic properties of these enzymes. In this study, we developed and optimized a fluorescence-based DNA ligation assay to characterize the activities of purified hLigs. Although hLigI joins DNA nicks, it has no detectable activity on linear duplex DNA substrates with short, cohesive single-strand ends. By contrast, hLigIII{beta} and the hLigIII{alpha}/XRCC1 and hLigIV/XRCC4 complexes are active on both nicked and linear duplex DNA substrates. Surprisingly, hLigIV/XRCC4, which is a key component of the major non-homologous end joining (NHEJ) pathway, is significantly less active than hLigIII on a linear duplex DNA substrate. Notably, hLigIV/XRCC4 molecules only catalyze a single ligation event in the absence or presence of ATP. The failure to catalyze subsequent ligation events reflects a defect in the enzyme-adenylation step of the next ligation reaction and suggests that, unless there is an in vivo mechanism to reactivate DNA ligase IV/XRCC4 following phosphodiester bond formation, the cellular NHEJ capacity will be determined by the number of adenylated DNA ligaseIV/XRCC4 molecules.

  19. Fast DNA Sequencing via Transverse Electronic Transport

    E-Print Network [OSTI]

    Johan Lagerqvist; Michael Zwolak; Massimiliano Di Ventra

    2006-03-01

    A rapid and low-cost method to sequence DNA would usher in a revolution in medicine. We propose and theoretically show the feasibility of a protocol for sequencing based on the distributions of transverse electrical currents of single-stranded DNA while it translocates through a nanopore. Our estimates, based on the statistics of these distributions, reveal that sequencing of an entire human genome could be done with very high accuracy in a matter of hours without parallelization, e.g., orders of magnitude faster than present techniques. The practical implementation of our approach would represent a substantial advancement in our ability to study, predict and cure diseases from the perspective of the genetic makeup of each individual.

  20. Determining orientation and direction of DNA sequences

    DOE Patents [OSTI]

    Goodwin, Edwin H. (Los Alamos, NM); Meyne, Julianne (Los Alamos, NM)

    2000-01-01

    Determining orientation and direction of DNA sequences. A method by which fluorescence in situ hybridization can be made strand specific is described. Cell cultures are grown in a medium containing a halogenated nucleotide. The analog is partially incorporated in one DNA strand of each chromatid. This substitution takes place in opposite strands of the two sister chromatids. After staining with the fluorescent DNA-binding dye Hoechst 33258, cells are exposed to long-wavelength ultraviolet light which results in numerous strand nicks. These nicks enable the substituted strand to be denatured and solubilized by heat, treatment with high or low pH aqueous solutions, or by immersing the strands in 2.times.SSC (0.3M NaCl+0.03M sodium citrate), to name three procedures. It is unnecessary to enzymatically digest the strands using Exo III or another exonuclease in order to excise and solubilize nucleotides starting at the sites of the nicks. The denaturing/solubilizing process removes most of the substituted strand while leaving the prereplication strand largely intact. Hybridization of a single-stranded probe of a tandem repeat arranged in a head-to-tail orientation will result in hybridization only to the chromatid with the complementary strand present.

  1. DNA repair decline during mouse spermiogenesis results in the accumulation of heritable DNA damage

    SciTech Connect (OSTI)

    Marchetti, Francesco; Marchetti, Francesco; Wryobek, Andrew J

    2008-02-21

    The post-meiotic phase of mouse spermatogenesis (spermiogenesis) is very sensitive to the genomic effects of environmental mutagens because as male germ cells form mature sperm they progressively lose the ability to repair DNA damage. We hypothesized that repeated exposures to mutagens during this repair-deficient phase result in the accumulation of heritable genomic damage in mouse sperm that leads to chromosomal aberrations in zygotes after fertilization. We used a combination of single or fractionated exposures to diepoxybutane (DEB), a component of tobacco smoke, to investigate how differential DNA repair efficiencies during the three weeks of spermiogenesis affected the accumulation of DEB-induced heritable damage in early spermatids (21-15 days before fertilization, dbf), late spermatids (14-8 dbf) and sperm (7- 1 dbf). Analysis of chromosomalaberrations in zygotic metaphases using PAINT/DAPI showed that late spermatids and sperm are unable to repair DEB-induced DNA damage as demonstrated by significant increases (P<0.001) in the frequencies of zygotes with chromosomal aberrations. Comparisons between single and fractionated exposures suggested that the DNA repair-deficient window during late spermiogenesis may be less than two weeks in the mouse and that during this repair-deficient window there is accumulation of DNA damage in sperm. Finally, the dose-response study in sperm indicated a linear response for both single and repeated exposures. These findings show that the differential DNA repair capacity of post-meioitic male germ cells has a major impact on the risk of paternally transmitted heritable damage and suggest that chronic exposures that may occur in the weeks prior to fertilization because of occupational or lifestyle factors (i.e, smoking) can lead to an accumulation of genetic damage in sperm and result in heritable chromosomal aberrations of paternal origin.

  2. DNA Repair Decline During Mouse Spermiogenesis Results in the Accumulation of Heritable DNA Damage

    SciTech Connect (OSTI)

    Marchetti, Francesco; Marchetti, Francesco; Wyrobek, Andrew J.

    2007-12-01

    The post-meiotic phase of mouse spermatogenesis (spermiogenesis) is very sensitive to the genomic effects of environmental mutagens because as male germ cells form mature sperm they progressively lose the ability to repair DNA damage. We hypothesized that repeated exposures to mutagens during this repair-deficient phase result in the accumulation of heritable genomic damage in mouse sperm that leads to chromosomal aberrations in zygotes after fertilization. We used a combination of single or fractionated exposures to diepoxybutane (DEB), a component of tobacco smoke, to investigate how differential DNA repair efficiencies during the three weeks of spermiogenesis affected the accumulation of DEB-induced heritable damage in early spermatids (21-15 days before fertilization, dbf), late spermatids (14-8 dbf) and sperm (7-1 dbf). Analysis of chromosomal aberrations in zygotic metaphases using PAINT/DAPI showed that late spermatids and sperm are unable to repair DEB-induced DNA damage as demonstrated by significant increases (P<0.001) in the frequencies of zygotes with chromosomal aberrations. Comparisons between single and fractionated exposures suggested that the DNA repair-deficient window during late spermiogenesis may be less than two weeks in the mouse and that during this repair-deficient window there is accumulation of DNA damage in sperm. Finally, the dose-response study in sperm indicated a linear response for both single and repeated exposures. These findings show that the differential DNA repair capacity of post-meioitic male germ cells has a major impact on the risk of paternally transmitted heritable damage and suggest that chronic exposures that may occur in the weeks prior to fertilization because of occupational or lifestyle factors (i.e, smoking) can lead to an accumulation of genetic damage in sperm and result in heritable chromosomal aberrations of paternal origin.

  3. Cytokine secretion profiles in primary cultured cells and in mice stimulated with plasmid DNA

    E-Print Network [OSTI]

    Yoshida, Hiroyuki

    2006-10-27

    University Elimination of DNA by antigen presenting cells Gene therapy Infection Leakage Processing Macrophages and dendritic cells Apoptotic or necrotic cells Endogenous DNA DNA Virus Plasmid DNA Exogenous DNA CpG motif5?- Pur Pur CG Pyr Pyr -3? Danger... signal Naked CpG DNA DNA lipoplex Macrophages or dendritic cells ???? ???? Cytokine geneNF-?B AP-1 ???? ???? Endosome Nucleus ? TLR9 Interferon ?/? gene Inflammatory cytokines TNF-?, IL-6 etc. Type I interferon IFN-?, INF-? ? Mechanisms of immune response...

  4. Capstan friction model for DNA ejection from bacteriophages

    E-Print Network [OSTI]

    Sandip Ghosal

    2013-01-09

    Bacteriophages infect cells by attaching to the outer membrane and injecting their DNA into the cell.The phage DNA is then transcribed by the cell's transcription machinery.A number of physical mechanisms by which DNA can be translocated from the phage capsid into the cell have been identified. A fast ejection driven by the elastic and electrostatic potential energy of the compacted DNA within the viral capsid appears to be used by most phages, at least to initiate infection.In recent in vitro experiments, the speed of DNA translocation from a lambda phage capsid has been measured as a function of ejected length over the entire duration of the event.Here a mechanical model is proposed that is able to explain the observed dependence of exit velocity on ejected length, and that is also consistent with the accepted picture of the geometric arrangement of DNA within the viral capsid.

  5. Flow cytometric measurement of total DNA and incorporated halodeoxyuridine

    DOE Patents [OSTI]

    Dolbeare, Frank A. (Livermore, CA); Gray, Joe W. (Livermore, CA)

    1988-01-01

    A method for the simultaneous flow cytometric measurement of the total DNA content and the level of DNA synthesis in normal and malignant cells is disclosed. The sensitivity of the method allows a study of cell cycle traverse rates for large scale cell populations as well as single cell measurements. A DNA stain such as propidium iodide or Hoechst 33258 is used as the probe for the measurement of total DNA content and a monoclonal antibody reactive with a DNA precursor such as halodeoxy-uridine (HdU), more specifically bromodeoxyuridine (BrdU) is used as a probe for the measurement of HdU or BrdU uptake by the cells as a measure of DNA synthesis.

  6. Bis[(1S)-1 4-azanediyl-1-(9-deazaadenin-9-yl)-1 4-dideoxy-5-methylsulfanyl-D-ribitol] tetrakis(hydrochloride) monohydrate: structure DFT energy and ligand docking results of a potent methylthioadenosine phosphorylase inhibitor found in different

    SciTech Connect (OSTI)

    G Gainsford; G Evans; K Johnston; M Seth

    2011-12-31

    The title compound, abbreviated as 5'ThiomethylImmA, is a potent inhibitor of methylthioadenosine phosphorylase [Singh et al. (2004). Biochemistry, 43, 9-18]. The synchrotron study reported here shows that the hydrochloride salt crystallizes with two independent, nearly superimposable, dications as a monohydrate with formula 2C{sub 12}H{sub 19}N{sub 5}O{sub 2}S{sup 2+}{center_dot}4Cl{sup -}{center_dot}H{sub 2}O. Hydrogen bonding utilizing the H atoms of the dication is found to favor certain molecular conformations in the salt, which are significantly different from those found as bound in the enzyme. Ligand docking studies starting from either of these dications or related neutral structures successfully place the conformationally revised structures in the enzyme active site but only under particular hydrogen-bonding and molecular flexibility criteria. Density functional theory calculations verify the energy similarity of the indendent cations and confirm the significant energy cost of the required conformation change to the enzyme bound form. The results suggest the using crystallographically determined free ligand coordinates as starting parameters for modelling may have serious limitations.

  7. Transposon-containing DNA cloning vector and uses thereof

    DOE Patents [OSTI]

    Berg, Claire M. (W. Willington, CT); Berg, Douglas E. (St. Louis, MO); Wang, Gan (Storrs, CT)

    1997-01-01

    The present invention discloses a rapid method of restriction mapping, sequencing or localizing genetic features in a segment of deoxyribonucleic acid (DNA) that is up to 42 kb in size. The method in part comprises cloning of the DNA segment in a specialized cloning vector and then isolating nested deletions in either direction in vivo by intramolecular transposition into the cloned DNA. A plasmid has been prepared and disclosed.

  8. Transposon-containing DNA cloning vector and uses thereof

    DOE Patents [OSTI]

    Berg, C.M.; Berg, D.E.; Wang, G.

    1997-07-08

    The present invention discloses a rapid method of restriction mapping, sequencing or localizing genetic features in a segment of deoxyribonucleic acid (DNA) that is up to 42 kb in size. The method in part comprises cloning of the DNA segment in a specialized cloning vector and then isolating nested deletions in either direction in vivo by intramolecular transposition into the cloned DNA. A plasmid has been prepared and disclosed. 4 figs.

  9. DNA Methylation as a Biomarker for Preeclampsia

    SciTech Connect (OSTI)

    Anderson, Cindy M.; Ralph, Jody L.; Wright, Michelle L.; Linggi, Bryan E.; Ohm, Joyce E.

    2014-10-01

    Background: Preeclampsia contributes significantly to pregnancy-associated morbidity and mortality as well as future risk of cardiovascular disease in mother and offspring, and preeclampsia in offspring. The lack of reliable methods for early detection limits the opportunities for prevention, diagnosis, and timely treatment. Purpose: The purpose of this study was to explore distinct DNA methylation patterns associated with preeclampsia in both maternal cells and fetal-derived tissue that represent potential biomarkers to predict future preeclampsia and inheritance in children. Method: A convenience sample of nulliparous women (N = 55) in the first trimester of pregnancy was recruited for this prospective study. Genome-wide DNA methylation was quantified in first-trimester maternal peripheral white blood cells and placental chorionic tissue from normotensive women and those with preeclampsia (n = 6/group). Results: Late-onset preeclampsia developed in 12.7% of women. Significant differences in DNA methylation were identified in 207 individual linked cytosine and guanine (CpG) sites in maternal white blood cells collected in the first trimester (132 sites with gain and 75 sites with loss of methylation), which were common to approximately 75% of the differentially methylated CpG sites identified in chorionic tissue of fetal origin. Conclusion: This study is the first to identify maternal epigenetic targets and common targets in fetal-derived tissue that represent putative biomarkers for early detection and heritable risk of preeclampsia. Findings may pave the way for diagnosis of preeclampsia prior to its clinical presentation and acute damaging effects, and the potential for prevention of the detrimental long-term sequelae.

  10. Allosteric Modulation of DNA by Small Molecules

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 OutreachProductswsicloudwsiclouddenDVA N C E D B L O O D S TA I N P A T TSorbentsAllosteric Modulation of DNA by

  11. DNA Origami: A History and Current Perspective

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home Room NewsInformation Current HAB Packet Hanford Advisory Board6/23/2014DLFM libraryDNA Origami

  12. A DNA tweezer-actuated enzyme nanoreactor

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of NaturalDukeWakefieldSulfateSciTechtail.TheoryTuesday, August 10, 20102016 News Below are4BElectron---neutrinoAA CleanAAA DNA

  13. Intriguing DNA Editor Has a Structural Trigger

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration would likeUniverseIMPACT EVALUATION PLANIsProcessRegulationRadiativeIntriguing DNA Editor Has a

  14. Intriguing DNA Editor Has a Structural Trigger

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration would likeUniverseIMPACT EVALUATION PLANIsProcessRegulationRadiativeIntriguing DNA Editor Has

  15. Ergonomics in DNA Sequencing: Standing Down to Ergonomics

    E-Print Network [OSTI]

    Naca, Christine

    2009-01-01

    Ergonomics in DNA SequencingStanding Down to Ergonomics Presented at the COEH 2009We Do: Introduction • How We Work: Ergonomics Challenges in

  16. High-Performance Integrated Genetic Analyzers for Forensic DNA Typing

    E-Print Network [OSTI]

    Liu, Peng

    2009-01-01

    in nanoliter volumes. Analytical Chemistry 72, 5507-5512 (genetic analysis. Analytical Chemistry 78, 5474-5479 (2006).DNA sequencing. Analytical Chemistry 78, 3632-3637 (2006).

  17. Effect of salt concentration on the stability of heterogeneous DNA

    E-Print Network [OSTI]

    Amar Singh; Navin Singh

    2015-09-28

    We study the role of cations on the stability of double stranded DNA (dsDNA) molecules.It is known that the two strands of double stranded DNA(dsDNA) have negative charge due to phosphate group. Cations in the form of salt in the solution, act as shielding agents thereby reducing the repulsion between these strands. We study several heterogeneous DNA molecules. We calculate the phase diagrams for DNA molecules in thermal as well as in force ensembles using Peyrard-Bishop-Dauxois (PBD) model. The dissociation and the stacking energies are the two most important factors that play an important role in the DNA stability. With suitable modifications in the model parameters we investigate the role of cation concentration on the stability of different heterogeneous DNA molecules. The objective of this work is to understand how these cations modify the strength of different pairs or bases along the strand. The phase diagram for the force ensemble case (a dsDNA is pulled from an end) is compared with the experimental results.

  18. Ionic switch controls the DNA state in phage ?

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Li, Dong; Liu, Ting; Zuo, Xiaobing; Li, Tao; Qiu, Xiangyun; Evilevitch, Alex

    2015-06-19

    We have recently found that DNA packaged in phage ? undergoes a disordering transition triggered by temperature, which results in increased genome mobility. This solid-to-fluid like DNA transition markedly increases the number of infectious ? particles facilitating infection. However, the structural transition strongly depends on temperature and ionic conditions in the surrounding medium. Using titration microcalorimetry combined with solution X-ray scattering, we mapped both energetic and structural changes associated with transition of the encapsidated ?-DNA. Packaged DNA needs to reach a critical stress level in order for transition to occur. We varied the stress on DNA in the capsid bymore »changing the temperature, packaged DNA length and ionic conditions. We found striking evidence that the intracapsid DNA transition is ‘switched on’ at the ionic conditions mimicking those in vivo and also at the physiologic temperature of infection at 37°C. This ion regulated on-off switch of packaged DNA mobility in turn affects viral replication. The results suggest a remarkable adaptation of phage ? to the environment of its host bacteria in the human gut. The metastable DNA state in the capsid provides a new paradigm for the physical evolution of viruses.« less

  19. Jefferson Lab Hosts Upcoming Science Lectures on DNA and Chocolate...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    lecture on March 29 titled DNA: The Strand That Connects Us All presented by Matt Kaplan from the Human Origins Genotyping Laboratory, Phoenix, Ariz. Kaplan will discuss how...

  20. Nanoscale topographical replication of graphene architecture by artificial DNA nanostructures

    SciTech Connect (OSTI)

    Moon, Y.; Seo, S.; Park, J.; Park, T.; Ahn, J. R., E-mail: jrahn@skku.edu [Department of Physics, Sungkyunkwan University, Suwon 440-746 (Korea, Republic of); Shin, J.; Dugasani, S. R. [Sungkyunkwan Advanced Institute of Nanotechnology (SAINT), Sungkyunkwan University, Suwon 440-746 (Korea, Republic of); Woo, S. H. [College of Pharmacy, Chungnam National University, Daejeon 305-764 (Korea, Republic of); Park, S. H., E-mail: sunghapark@skku.edu [Department of Physics, Sungkyunkwan University, Suwon 440-746 (Korea, Republic of); Sungkyunkwan Advanced Institute of Nanotechnology (SAINT), Sungkyunkwan University, Suwon 440-746 (Korea, Republic of)

    2014-06-09

    Despite many studies on how geometry can be used to control the electronic properties of graphene, certain limitations to fabrication of designed graphene nanostructures exist. Here, we demonstrate controlled topographical replication of graphene by artificial deoxyribonucleic acid (DNA) nanostructures. Owing to the high degree of geometrical freedom of DNA nanostructures, we controlled the nanoscale topography of graphene. The topography of graphene replicated from DNA nanostructures showed enhanced thermal stability and revealed an interesting negative temperature coefficient of sheet resistivity when underlying DNA nanostructures were denatured at high temperatures.

  1. Fleet DNA Project - Data Dictionary for Public Download Files

    SciTech Connect (OSTI)

    Duran, A.; Burton, E.; Kelly, K.; Walkowicz, K.

    2014-09-01

    Reference document for the Fleet DNA results data shared on the NREL public website. The document includes variable definitions and descriptions to assist users in understanding data.

  2. Selective chemical labelling of natural T modifications in DNA

    E-Print Network [OSTI]

    Hardisty, Robyn E.; Kawasaki, Fumiko; Sahakyan, Aleksandr B.; Balasubramanian, Shankar

    2015-05-06

    enriched, we carried out experiments exploiting the selective reactions developed for probes 1, 2, and 3. A double-stranded 80-mer bearing two modifications per strand was used as a model for 5-fU (fU-DNA), while an analogous ODN containing 5-fC (f... C-DNA) and a non-modified ODN (GCAT-DNA) were used as controls (Supporting Information, Table S1). These ODNs were subjected to the biotinylation reaction followed by affinity enrichment using streptavidin- coated magnetic beads. fU-DNA was enriched over f...

  3. Validation of DNA probes for molecular cytogenetics by mapping onto immobilized circular DNA

    SciTech Connect (OSTI)

    Greulich-Bode, Karin M.; Wang, Mei; Rhein, Andreas P.; Weier, Jingly F.; Weier, Heinz-Ulli G.

    2008-12-04

    Fluorescence in situ hybridization (FISH) is a sensitive and rapid procedure to detect gene rearrangements in tumor cells using non-isotopically labeled DNA probes. Large insert recombinant DNA clones such as bacterial artificial chromosome (BAC) or P1/PAC clones have established themselves in recent years as preferred starting material for probe preparations due to their low rates of chimerism and ease of use. However, when developing probes for the quantitative analysis of rearrangements involving genomic intervals of less than 100kb, careful probe selection and characterization are of paramount importance. We describe a sensitive approach to quality control probe clones suspected of carrying deletions or for measuring clone overlap with near kilobase resolution. The method takes advantage of the fact that P1/PAC/BAC's can be isolated as circular DNA molecules, stretched out on glass slides and fine-mapped by multicolor hybridization with smaller probe molecules. Two examples demonstrate the application of this technique: mapping of a gene-specific {approx}6kb plasmid onto an unusually small, {approx}55kb circular P1 molecule and the determination of the extent of overlap between P1 molecules homologous to the human NF-{kappa}B2 locus. The relatively simple method presented here does not require specialized equipment and may thus find widespread applications in DNA probe preparation and characterization, the assembly of physical maps for model organisms or in studies on gene rearrangements.

  4. Validation of DNA probes for molecular cytogenetics by mapping onto immobilized circular DNA

    SciTech Connect (OSTI)

    Greulich-Bode, Karin; Wang, Mei; Rhein, Andreas; Weier, Jingly; Weier, Heinz-Ulli

    2008-12-16

    Fluorescence in situ hybridization (FISH) is a sensitive and rapid procedure to detect gene rearrangements in tumor cells using non-isotopically labeled DNA probes. Large insert recombinant DNA clones such as bacterial artificial chromosome (BAC) or P1/PAC clones have established themselves in recent years as preferred starting material for probe preparations due to their low rates of chimerism and ease of use. However, when developing probes for the quantitative analysis of rearrangements involving genomic intervals of less than 100kb, careful probe selection and characterization are of paramount importance. We describe a sensitive approach to quality control probe clones suspected of carrying deletions or for measuring clone overlap with near kilobase resolution. The method takes advantage of the fact that P1/PAC/BAC's can be isolated as circular DNA molecules, stretched out on glass slides and fine-mapped by multicolor hybridization with smaller probe molecules. Two examples demonstrate the application of this technique: mapping of a gene-specific {approx}6kb plasmid onto an unusually small, {approx}55kb circular P1 molecule and the determination of the extent of overlap between P1 molecules homologous to the human NF-?B2 locus. The relatively simple method presented here does not require specialized equipment and may thus find widespread applications in DNA probe preparation and characterization, the assembly of physical maps for model organisms or in studies on gene rearrangements.

  5. Physical properties of naked DNA influence nucleosome positioning and correlate with transcription start and termination sites in yeast

    E-Print Network [OSTI]

    Deniz, Özgen; Flores, Oscar; Battistini, Federica; Pérez, Alberto; Soler-López, Montserrat; Orozco, Modesto

    2011-01-01

    al. : Physical properties of naked DNA influence nucleosomeAccess Physical properties of naked DNA influence nucleosomeelusive. Results: Naked (histone-free) and nucleosomal DNA

  6. DNA-Based Optomechanical Molecular Motor

    SciTech Connect (OSTI)

    McCullagh, Martin; Franco, Ignacio; Ratner, Mark A.; Schatz, George C.

    2011-03-16

    An azobenzene-capped DNA hairpin coupled to an AFM is presented as an optically triggered single-molecule motor. The photoinduced trans to cis isomerization of azobenzene affects both the overall length of the molecule and the ability of the DNA bases to hybridize. Using a combination of molecular dynamics simulations and free energy calculations the unfolding of both isomers along the O5'-O3' extension coordinate is monitored. The potentials of mean force (PMFs) along this coordinate indicate that there are two major differences induced by photoisomerization. The first is that the interbase hydrogen bond and stacking interactions are stable for a greater range of extensions in the trans system than in the cis system. The second difference is due to a decreased chain length of the cis isomer with respect to the trans isomer. These differences are exploited to extract work in optomechanical cycles. The disruption of the hairpin structure gives a maximum of 3.4 kcal mol-1 of extractable work per cycle with an estimated maximum efficiency of 2.4%. Structure-function insights into the operation of this motor are provided, and the effect of the cantilever stiffness on the extractable work is characterized.

  7. Force steps during viral DNA packaging ?

    E-Print Network [OSTI]

    Prashant K. Purohit; Jane' Kondev; Rob Phillips

    2003-09-22

    Biophysicists and structural biologists increasingly acknowledge the role played by the mechanical properties of macromolecules as a critical element in many biological processes. This change has been brought about, in part, by the advent of single molecule biophysics techniques that have made it possible to exert piconewton forces on key macromolecules and observe their deformations at nanometer length scales, as well as to observe the mechanical action of macromolecules such as molecular motors. This has opened up immense possibilities for a new generation of mechanical investigations that will respond to such measurements in an attempt to develop a coherent theory for the mechanical behavior of macromolecules under conditions where thermal and chemical effects are on an equal footing with deterministic forces. This paper presents an application of the principles of mechanics to the problem of DNA packaging, one of the key events in the life cycle of bacterial viruses with special reference to the nature of the internal forces that are built up during the DNA packaging process.

  8. ORIGINAL ARTICLE Forensic DNA databasesEthical and legal

    E-Print Network [OSTI]

    Noble, William Stafford

    number of innocent people's records to be kept, became highly controversial.1­3 * Corresponding author Authority. #12;Under former UK Prime Minister Tony Blair, legislation was introduced as part of the Criminal's DNA profiles to be retained on what was previously a criminal DNA database, overturning the pre

  9. Molecular Devices A Unidirectional DNA Walker That Moves

    E-Print Network [OSTI]

    Yin, Peng

    a designated path. The successful construction of self-assembled DNA nanostructures provides a solid structural,2] In particular, recent years have seen remarkable success in the construction of both self-assembled that moves along a DNA track. The self-assembled track contains three anchor- ages at which the walker, a six

  10. Alternative Methods for Human Identification: Mitochondrial DNA Base Composition Profiling

    E-Print Network [OSTI]

    Perkins, Richard A.

    of nucleotides ­ Base composition of a DNA molecule can be inferred ­ An empirical formula of numbers of A, G, C) · Fully automated ­ Plate stacker holds up to 15 PCR plates ­ Desalting by magnetic bead cleanup · Cleanup are dissociated on injection · DNA molecular masses are measured ­ Forward and reverse strands measured separately

  11. DNA damage in cells exhibiting radiation-induced genomic instability

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Keszenman, Deborah J.; Kolodiuk, Lucia; Baulch, Janet E.

    2015-02-22

    Cells exhibiting radiation induced genomic instability exhibit varied spectra of genetic and chromosomal aberrations. Even so, oxidative stress remains a common theme in the initiation and/or perpetuation of this phenomenon. Isolated oxidatively modified bases, abasic sites, DNA single strand breaks and clustered DNA damage are induced in normal mammalian cultured cells and tissues due to endogenous reactive oxygen species generated during normal cellular metabolism in an aerobic environment. While sparse DNA damage may be easily repaired, clustered DNA damage may lead to persistent cytotoxic or mutagenic events that can lead to genomic instability. In this study, we tested the hypothesismore »that DNA damage signatures characterised by altered levels of endogenous, potentially mutagenic, types of DNA damage and chromosomal breakage are related to radiation-induced genomic instability and persistent oxidative stress phenotypes observed in the chromosomally unstable progeny of irradiated cells. The measurement of oxypurine, oxypyrimidine and abasic site endogenous DNA damage showed differences in non-double-strand breaks (DSB) clusters among the three of the four unstable clones evaluated as compared to genomically stable clones and the parental cell line. These three unstable clones also had increased levels of DSB clusters. The results of this study demonstrate that each unstable cell line has a unique spectrum of persistent damage and lead us to speculate that alterations in DNA damage signaling and repair may be related to the perpetuation of genomic instability.« less

  12. Redesigning the Specificity of ProteinDNA Interactions with Rosetta

    E-Print Network [OSTI]

    Baker, David

    +Business Media New York 2014 #12;266 DNA cleavage enzymes, such as LAGLIDADG endonucleases [4, 5], to sites specificity offer an efficient alternative to more labor-intensive experimental procedures, such as directed­DNA specificity.Each panel represents the structure with the mean energy from a set of 56 repacks done

  13. DNA topology confers sequence specificity to nonspecific architectural proteins

    E-Print Network [OSTI]

    Swigon, David

    DNA topology confers sequence specificity to nonspecific architectural proteins Juan Weia , Luke proteins into tightly organized 3D struc- tures. The bacterial heat-unstable (HU) protein builds up. The HU protein introduces a unique spatial pathway in the DNA upon closure. The many ways in which

  14. Short communication The evolution of DNA databases--Recommendations

    E-Print Network [OSTI]

    Short communication The evolution of DNA databases--Recommendations for new European STR loci Peter from 7 to 10. # 2005 Elsevier Ireland Ltd. All rights reserved. Keywords: National DNA database; STR; Evolution; European standards 1. Introduction The European Network of Forensic Science Institutes (ENFSI

  15. Shape and energetics of DNA plectonemes Prashant K. Purohit

    E-Print Network [OSTI]

    Purohit, Prashant

    , including single molecule experiments, and it is known that the average bending modulus of a random sequence scales of few hundred nanometers is described by a fluctuating elastic rod model. In this paper we couple that the twisting modulus of DNA is around Kt = 431pNnm2 [12]. The fact that DNA is a twist storing polymer allows

  16. cDNA encoding a polypeptide including a hevein sequence

    DOE Patents [OSTI]

    Raikhel, N.V.; Broekaert, W.F.; Namhai Chua; Kush, A.

    1993-02-16

    A cDNA clone (HEV1) encoding hevein was isolated via polymerase chain reaction (PCR) using mixed oligonucleotides corresponding to two regions of hevein as primers and a Hevea brasiliensis latex cDNA library as a template. HEV1 is 1,018 nucleotides long and includes an open reading frame of 204 amino acids.

  17. DNA barcoding and the renaissance of taxonomy Scott E. Miller*

    E-Print Network [OSTI]

    DNA barcoding and the renaissance of taxonomy Scott E. Miller* Office of the Under Secretary representing field ecology, molecular genetics, and morphological taxonomy presents data in this issue of PNAS). The work of Smith et al. (2) is a display of integrated taxonomy, demon- strating how DNA barcoding

  18. Google matrix analysis of DNA sequences Vivek Kandiah1

    E-Print Network [OSTI]

    Shepelyansky, Dima

    1 Google matrix analysis of DNA sequences Vivek Kandiah1 , Dima L. Shepelyansky1, 1 Laboratoire de.quantware.ups-tlse.fr/dima Abstract For DNA sequences of various species we construct the Google matrix G of Markov tran- sitions between nearby words composed of several letters. The statistical distribution of matrix elements

  19. Semiflexible Polymers: Fundamental Theory and Applications in DNA Packaging

    E-Print Network [OSTI]

    Straight, Aaron

    Semiflexible Polymers: Fundamental Theory and Applications in DNA Packaging Thesis by Andrew James of perfectly flexible and perfectly rigid polymer chains; however, many polymers, for example DNA, are somewhere in between these two limiting cases. Such polymers are termed semiflexible, and their molecular

  20. 2006 Nature Publishing Group Mechanochemical analysis of DNA gyrase using

    E-Print Network [OSTI]

    Gore, Jeff

    -independent step at the end of the reaction cycle, and an ATP-binding step in the middle of the cycle, subsequent of DNA gyrase, which works against mechanical stresses to drive the genome into an elastically strained releasing the DNA substrate), the location of the rate-limiting step for the overall reaction cycle

  1. Investigating the Role of Vitamin D and DNA Repair in Influencing Cancer Presentation and Outcomes 

    E-Print Network [OSTI]

    Syed, Moinuddin Mohammed

    2014-09-26

    differences in DNA repair genes exist, which may in turn contribute to underlying differences in DNA repair capacity. These genes with high Fst value represent all six known common DNA repair pathways and DDR response. This supports our hypothesis...

  2. The role of the de novo DNA methyltransferase Dnmt3a in the nervous system

    E-Print Network [OSTI]

    Nguyen, Suzanne Pham

    2007-01-01

    DNA methylation is an important mechanism of gene regulation. Evidence is mounting that epigenetic mechanisms including that of DNA methylation operate in the nervous system. Genetic disruption of the de novo DNA ...

  3. Telomere Regulation in Arabidopsis thaliana by the CST Capping Complex and DNA Damage Response Proteins 

    E-Print Network [OSTI]

    Boltz, Kara A.

    2013-09-11

    The ends of chormosomes are capped by telomeres, which distinguish the termini from damaged DNA. Paradoxically, DNA repair proteins are also required for telomere maintenance. How DNA repair pathways are regulated to ...

  4. Morphological taxonomy, DNA barcoding, and species diversity in southern Rocky Mountain headwater streams

    E-Print Network [OSTI]

    Zamudio, Kelly R.

    Morphological taxonomy, DNA barcoding, and species diversity in southern Rocky Mountain headwater and Conditions #12;MOLECULAR APPROACHES IN FRESHWATER ECOLOGY Morphological taxonomy, DNA barcoding, and species: diversity, elevation, DNA barcoding, taxonomy, aquatic insect, EPT, southern Rocky Mountain Elevation

  5. Extending the realm of SuNS to DNA nanoarrays and peptide features

    E-Print Network [OSTI]

    Akbulut Halatci, Özge

    2010-01-01

    Intense research on DNA arrays has been fostered by their applications in the field of biomedicine. DNA microarrays are composed of several different DNA sequences to be analyzed in parallel allowing high throughput ...

  6. Structure of DNA-Bound FEN1 Reveals Mechanism of Action

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Structure of DNA-Bound FEN1 Reveals Mechanism of Action Structure of DNA-Bound FEN1 Reveals Mechanism of Action Print Tuesday, 24 January 2012 11:30 DNA replication is a critical...

  7. Hybridization and Selective Release of DNA Microarrays

    SciTech Connect (OSTI)

    Beer, N R; Baker, B; Piggott, T; Maberry, S; Hara, C M; DeOtte, J; Benett, W; Mukerjee, E; Dzenitis, J; Wheeler, E K

    2011-11-29

    DNA microarrays contain sequence specific probes arrayed in distinct spots numbering from 10,000 to over 1,000,000, depending on the platform. This tremendous degree of multiplexing gives microarrays great potential for environmental background sampling, broad-spectrum clinical monitoring, and continuous biological threat detection. In practice, their use in these applications is not common due to limited information content, long processing times, and high cost. The work focused on characterizing the phenomena of microarray hybridization and selective release that will allow these limitations to be addressed. This will revolutionize the ways that microarrays can be used for LLNL's Global Security missions. The goals of this project were two-fold: automated faster hybridizations and selective release of hybridized features. The first study area involves hybridization kinetics and mass-transfer effects. the standard hybridization protocol uses an overnight incubation to achieve the best possible signal for any sample type, as well as for convenience in manual processing. There is potential to significantly shorten this time based on better understanding and control of the rate-limiting processes and knowledge of the progress of the hybridization. In the hybridization work, a custom microarray flow cell was used to manipulate the chemical and thermal environment of the array and autonomously image the changes over time during hybridization. The second study area is selective release. Microarrays easily generate hybridization patterns and signatures, but there is still an unmet need for methodologies enabling rapid and selective analysis of these patterns and signatures. Detailed analysis of individual spots by subsequent sequencing could potentially yield significant information for rapidly mutating and emerging (or deliberately engineered) pathogens. In the selective release work, optical energy deposition with coherent light quickly provides the thermal energy to single spots to release hybridized DNA. This work leverages LLNL expertise in optics, microfluids, and bioinformatics.

  8. A novel role for the transcriptional modulator NusA in DNA repair/damage tolerance pathways in Escherichia coli

    E-Print Network [OSTI]

    Cohen, Susan E., Ph. D. Massachusetts Institute of Technology

    2009-01-01

    All organisms must contend with the consequences of DNA damage, induced by a variety of both endogenous and exogenous sources. Mechanisms of DNA repair and DNA damage tolerance are crucial for cellular survival after DNA ...

  9. Statistical Mutation Calling from Sequenced Overlapping DNA Pools in TILLING Experiments

    E-Print Network [OSTI]

    Missirian, Victor; Comai, Luca; Filkov, Vladimir

    2011-01-01

    S, Pe’er I: Overlapping pools for high-throughput targetedSequenced Overlapping DNA Pools in TILLING Experiments. BMCgeneration resequencing of DNA pools. Bioinformatics 2010,

  10. Sensitive method for measurement of telomeric DNA content in human tissues

    DOE Patents [OSTI]

    Bryant, Jennifer E. (Albuquerque, NM); Hutchings, Kent G. (Albuquerque, NM); Moyzis, Robert K. (Corona Del Mar, CA); Griffith, Jeffrey K. (Cedar Crest, NM)

    1999-02-16

    A sensitive method for measurement of telomeric DNA content in human tissue, based upon the ratio of telomeric to centromeric DNA present in the tissue.

  11. DNA repair: Dynamic defenders against cancer and aging

    SciTech Connect (OSTI)

    Fuss, Jill O.; Cooper, Priscilla K.

    2006-04-01

    You probably weren't thinking about your body's cellular DNA repair systems the last time you sat on the beach in the bright sunshine. Fortunately, however, while you were subjecting your DNA to the harmful effects of ultraviolet light, your cells were busy repairing the damage. The idea that our genetic material could be damaged by the sun was not appreciated in the early days of molecular biology. When Watson and Crick discovered the structure of DNA in 1953 [1], it was assumed that DNA is fundamentally stable since it carries the blueprint of life. However, over 50 years of research have revealed that our DNA is under constant assault by sunlight, oxygen, radiation, various chemicals, and even our own cellular processes. Cleverly, evolution has provided our cells with a diverse set of tools to repair the damage that Mother Nature causes. DNA repair processes restore the normal nucleotide sequence and DNA structure of the genome after damage [2]. These responses are highly varied and exquisitely regulated. DNA repair mechanisms are traditionally characterized by the type of damage repaired. A large variety of chemical modifications can alter normal DNA bases and either lead to mutations or block transcription if not repaired, and three distinct pathways exist to remove base damage. Base excision repair (BER) corrects DNA base alterations that do not distort the overall structure of the DNA helix such as bases damaged by oxidation resulting from normal cellular metabolism. While BER removes single damaged bases, nucleotide excision repair (NER) removes short segments of nucleotides (called oligonucleotides) containing damaged bases. NER responds to any alteration that distorts the DNA helix and is the mechanism responsible for repairing bulky base damage caused by carcinogenic chemicals such as benzo [a]pyrene (found in cigarette smoke and automobile exhaust) as well as covalent linkages between adjacent pyrimidine bases resulting from the ultraviolet (UV) component of sunlight. NER can be divided into two classes based on where the repair occurs. NER occurring in DNA that is not undergoing transcription (i.e., most of the genome) is called global genome repair (GGR or GGNER), while NER taking place in the transcribed strand of active genes is called transcription-coupled repair (TCR or TC-NER). We will explore NER in more detail below. Mismatch repair (MMR) is another type of excision repair that specifically removes mispaired bases resulting from replication errors. DNA damage can also result in breaks in the DNA backbone, in one or both strands. Single-strand breaks (SSBs) are efficiently repaired by a mechanism that shares common features with the later steps in BER. Double-strand breaks (DSBs) are especially devastating since by definition there is no intact complementary strand to serve as a template for repair, and even one unrepaired DSB can be lethal [3]. In cells that have replicated their DNA prior to cell division, the missing information can be supplied by the duplicate copy, or sister chromatid, and DSBs in these cells are faithfully repaired by homologous recombination involving the exchange of strands of DNA between the two copies. However, most cells in the body are non-dividing, and in these cells the major mechanism for repairing DSBs is by non-homologous end joining (NHEJ), which as the name implies involves joining two broken DNA ends together without a requirement for homologous sequence and which therefore has a high potential for loss of genetic information.

  12. An intercalation-locked parallel-stranded DNA tetraplex

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Tripathi, S.; Zhang, D.; Paukstelis, P. J.

    2015-01-27

    DNA has proved to be an excellent material for nanoscale construction because complementary DNA duplexes are programmable and structurally predictable. However, in the absence of Watson–Crick pairings, DNA can be structurally more diverse. Here, we describe the crystal structures of d(ACTCGGATGAT) and the brominated derivative, d(ACBrUCGGABrUGAT). These oligonucleotides form parallel-stranded duplexes with a crystallographically equivalent strand, resulting in the first examples of DNA crystal structures that contains four different symmetric homo base pairs. Two of the parallel-stranded duplexes are coaxially stacked in opposite directions and locked together to form a tetraplex through intercalation of the 5'-most A–A base pairs betweenmore »adjacent G–G pairs in the partner duplex. The intercalation region is a new type of DNA tertiary structural motif with similarities to the i-motif. 1H–1H nuclear magnetic resonance and native gel electrophoresis confirmed the formation of a parallel-stranded duplex in solution. Finally, we modified specific nucleotide positions and added d(GAY) motifs to oligonucleotides and were readily able to obtain similar crystals. This suggests that this parallel-stranded DNA structure may be useful in the rational design of DNA crystals and nanostructures.« less

  13. Extreme bendability of DNA double helix due to bending asymmetry

    E-Print Network [OSTI]

    Hossein Salari; B. Eslami-Mossallam; M. S. Naderi; M. R. Ejtehadi

    2015-05-23

    Experimental data of the DNA cyclization (J-factor) at short length scales, as a way to study the elastic behavior of tightly bent DNA, exceed the theoretical expectation based on the wormlike chain (WLC) model by several orders of magnitude. Here, we propose that asymmetric bending rigidity of the double helix in the groove direction can be responsible for extreme bendability of DNA at short length scales and it also facilitates DNA loop formation at these lengths. To account for the bending asymmetry, we consider the asymmetric elastic rod (AER) model which has been introduced and parametrized in an earlier study (B. Eslami-Mossallam and M. Ejtehadi, Phys. Rev. E 80, 011919 (2009)). Exploiting a coarse grained representation of DNA molecule at base pair (bp) level, and using the Monte Carlo simulation method in combination with the umbrella sampling technique, we calculate the loop formation probability of DNA in the AER model. We show that, for DNA molecule has a larger J-factor compared to the WLC model which is in excellent agreement with recent experimental data.

  14. Osmotic pressure: resisting or promoting DNA ejection from phage

    E-Print Network [OSTI]

    Meerim Jeembaeva; Martin Castelnovo; Frida Larsson; Alex Evilevitch

    2008-06-24

    Recent in vitro experiments have shown that DNA ejection from bacteriophage can be partially stopped by surrounding osmotic pressure when ejected DNA is digested by DNase I on the course of ejection. We argue in this work by combination of experimental techniques (osmotic suppression without DNaseI monitored by UV absorbance, pulse-field electrophoresis, and cryo-EM visualization) and simple scaling modeling that intact genome (i.e. undigested) ejection in a crowded environment is, on the contrary, enhanced or eventually complete with the help of a pulling force resulting from DNA condensation induced by the osmotic stress itself. This demonstrates that in vivo, the osmotically stressed cell cytoplasm will promote phage DNA ejection rather than resisting it. The further addition of DNA-binding proteins under crowding conditions is shown to enhance the extent of ejection. We also found some optimal crowding conditions for which DNA content remaining in the capsid upon ejection is maximum, which correlates well with the optimal conditions of maximum DNA packaging efficiency into viral capsids observed almost 20 years ago. Biological consequences of this finding are discussed.

  15. Extreme bendability of DNA double helix due to bending asymmetry

    E-Print Network [OSTI]

    Salari, Hossein; Naderi, M S; Ejtehadi, M R

    2015-01-01

    Experimental data of the DNA cyclization (J-factor) at short length scales, as a way to study the elastic behavior of tightly bent DNA, exceed the theoretical expectation based on the wormlike chain (WLC) model by several orders of magnitude. Here, we propose that asymmetric bending rigidity of the double helix in the groove direction can be responsible for extreme bendability of DNA at short length scales and it also facilitates DNA loop formation at these lengths. To account for the bending asymmetry, we consider the asymmetric elastic rod (AER) model which has been introduced and parametrized in an earlier study (B. Eslami-Mossallam and M. Ejtehadi, Phys. Rev. E 80, 011919 (2009)). Exploiting a coarse grained representation of DNA molecule at base pair (bp) level, and using the Monte Carlo simulation method in combination with the umbrella sampling technique, we calculate the loop formation probability of DNA in the AER model. We show that, for DNA molecule has a larger J-factor compared to the WLC model w...

  16. Specificity, flexibility and valence of DNA bonds guide emulsion architecture

    E-Print Network [OSTI]

    Lang Feng; Lea-Laetitia Pontani; Remi Dreyfus; Paul Chaikin; Jasna Brujic

    2013-03-25

    The specificity and thermal reversibility of DNA interactions have enabled the self-assembly of crystal structures, self-replicating materials and colloidal molecules. Grafting DNA onto liquid interfaces of emulsions leads to exciting new architectural possibilities due to the mobility of the DNA ligands and the patches they form between bound droplets. Here we show that the size and number of these adhesion patches (valency) can be controlled. Valence 2 leads to flexible polymers of emulsion droplets, while valence above 4 leads to rigid droplet networks. A simple thermodynamic model quantitatively describes the increase in the patch size with droplet radii, DNA concentration and the stiffness of the tether to the sticky-end. The patches are formed between droplets with complementary DNA strands or alternatively with complementary colloidal nanoparticles to mediate DNA binding between droplets. This emulsion system opens the route to directed self-assembly of more complex structures through distinct DNA bonds with varying strengths and controlled valence and flexibility.

  17. Method for construction of normalized cDNA libraries

    DOE Patents [OSTI]

    Soares, M.B.; Efstratiadis, A.

    1996-01-09

    This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form. The method comprises: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3` noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library. 4 figs.

  18. Method for construction of normalized cDNA libraries

    DOE Patents [OSTI]

    Soares, Marcelo B. (New York, NY); Efstratiadis, Argiris (Englewood, NJ)

    1996-01-01

    This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3' noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library.

  19. DNA banking and DNA databanking: Legal, ethical, and public policy issues. Progress report, [April 1, 1993--March 31, 1994

    SciTech Connect (OSTI)

    Reilly, P.R.; McEwen, J.E.; Small, D.

    1994-02-18

    The purpose of the grant was to provide support to enable us to: (1) perform legal and empirical research and critically analyze DNA banking and DNA databanking as those activities are conducted by state forensic laboratories, the military, academic researchers, and commercial enterprises; and (2) develop a broadcast quality educational videotape for viewing by the general public about DNA technology and the privacy and related issues that it raises. The grant thus has both a research and analysis component and a public education component. This report outlines the work completed since the inception of the project and describes the activities still in progress.

  20. Cellular responses against DNA damaged by platinum anticancer drugs

    E-Print Network [OSTI]

    Jung, Yongwon, 1977-

    2005-01-01

    The anticancer activity of platinum-based drugs such as cisplatin, carboplatin, and oxaliplatin is mediated by their ability to attack DNA such that generated adducts trigger numerous cellular responses. A better understanding ...

  1. GATA 8(1): 1-7, 1991 DNA SEQUENCING

    E-Print Network [OSTI]

    Davis, Lloyd M.

    of DNA must be built up from many overlapping subsequences, 200-600 bases long. With the overlap with the disposal of radioac- tive materials and other hazardous wastes, such as acrylamide, which plague current

  2. When DNA Needs to Stand Up and Be Counted

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    biowarfare agents. If this sounds like science fiction, it is only because the latest biotech marvel -the "DNA chip"-still needs a few more years to become part of everyday life....

  3. An integrated encyclopedia of DNA elements in the human genome

    E-Print Network [OSTI]

    Altshuler, Robert Charles

    The human genome encodes the blueprint of life, but the function of the vast majority of its nearly three billion bases is unknown. The Encyclopedia of DNA Elements (ENCODE) project has systematically mapped regions of ...

  4. Development of a microfluidic platform for integrated DNA sequencing protocols

    E-Print Network [OSTI]

    Kumar, Mayank, S.M. Massachusetts Institute of Technology

    2007-01-01

    This thesis describes the design and development of a microfluidic platform to reduce costs and improve the quality of in the DNA sequencing methodology currently implemented at the Broad Institute in Cambridge, Massachusetts. ...

  5. Single-Pair FRET Characterization of DNA Tweezers

    E-Print Network [OSTI]

    Ludwig-Maximilians-Universität, München

    Single-Pair FRET Characterization of DNA Tweezers Barbara K. Mu1ller, Andreas Reuter, Friedrich C-Champaign, 1110 West Green Street, Urbana, Illinois 61801 Received August 18, 2006; Revised Manuscript Received

  6. Method for construction of normalized cDNA libraries

    DOE Patents [OSTI]

    Soares, M.B.; Efstratiadis, A.

    1998-11-03

    This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3` noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to appropriate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library. This invention also provides normalized cDNA libraries generated by the above-described method and uses of the generated libraries. 19 figs.

  7. Method for rapid base sequencing in DNA and RNA

    DOE Patents [OSTI]

    Jett, J.H.; Keller, R.A.; Martin, J.C.; Moyzis, R.K.; Ratliff, R.L.; Shera, E.B.; Stewart, C.C.

    1987-10-07

    A method is provided for the rapid base sequencing of DNA or RNA fragments wherein a single fragment of DNA or RNA is provided with identifiable bases and suspended in a moving flow stream. An exonuclease sequentially cleaves individual bases from the end of the suspended fragment. The moving flow stream maintains the cleaved bases in an orderly train for subsequent detection and identification. In a particular embodiment, individual bases forming the DNA or RNA fragments are individually tagged with a characteristic fluorescent dye. The train of bases is then excited to fluorescence with an output spectrum characteristic of the individual bases. Accordingly, the base sequence of the original DNA or RNA fragment can be reconstructed. 2 figs.

  8. Method for rapid base sequencing in DNA and RNA

    DOE Patents [OSTI]

    Jett, J.H.; Keller, R.A.; Martin, J.C.; Moyzis, R.K.; Ratliff, R.L.; Shera, E.B.; Stewart, C.C.

    1990-10-09

    A method is provided for the rapid base sequencing of DNA or RNA fragments wherein a single fragment of DNA or RNA is provided with identifiable bases and suspended in a moving flow stream. An exonuclease sequentially cleaves individual bases from the end of the suspended fragment. The moving flow stream maintains the cleaved bases in an orderly train for subsequent detection and identification. In a particular embodiment, individual bases forming the DNA or RNA fragments are individually tagged with a characteristic fluorescent dye. The train of bases is then excited to fluorescence with an output spectrum characteristic of the individual bases. Accordingly, the base sequence of the original DNA or RNA fragment can be reconstructed. 2 figs.

  9. Facilitated diffusion on mobile DNA: configurational traps and sequence heterogeneity

    E-Print Network [OSTI]

    C. A. Brackley; M. E. Cates; D. Marenduzzo

    2012-12-07

    We present Brownian dynamics simulations of the facilitated diffusion of a protein, modelled as a sphere with a binding site on its surface, along DNA, modelled as a semi-flexible polymer. We consider both the effect of DNA organisation in 3D, and of sequence heterogeneity. We find that in a network of DNA loops, as are thought to be present in bacterial DNA, the search process is very sensitive to the spatial location of the target within such loops. Therefore, specific genes might be repressed or promoted by changing the local topology of the genome. On the other hand, sequence heterogeneity creates traps which normally slow down facilitated diffusion. When suitably positioned, though, these traps can, surprisingly, render the search process much more efficient.

  10. DNA Duplication Revealed in New Beginnings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    to wrap around and bend approximately 70 base pairs of double stranded DNA (red and blue). When a replication initiator Cdc6 (green) joins ORC, the partial ring is now complete...

  11. The Fleet DNA Project (Fact Sheet), NREL (National Renewable...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of market-ready technologies that reduce costs, fuel consumption, and emissions. At the heart of the Fleet DNA Project is a clearinghouse of medium- and heavy-duty commercial fleet...

  12. Developing a DNA vaccine to protect against brucellosis 

    E-Print Network [OSTI]

    Owen, David Matthew

    2013-02-22

    Brucella are Gram-negative intracellular pathogenic bacteria, which represent a threat to human and animal health. Live vaccine strains are available to protect some animal species but no vaccines exist for human use. A DNA vaccine could potentially...

  13. Optical studies of DNA-wrapped carbon nanotubes

    E-Print Network [OSTI]

    Chou, Shin Grace

    2006-01-01

    This thesis presents a series of detailed optical studies of phonon-assisted relaxation processes in DNA-wrapped single walled carbon nanotubes. Using resonance Raman spectroscopy (RRS) and photoluminescence spectroscopy ...

  14. Flow cytomeric measurement of DNA and incorporated nucleoside analogs

    DOE Patents [OSTI]

    Dolbeare, Frank A. (Livermore, CA); Gray, Joe W. (Livermore, CA)

    1989-01-01

    A method is provided for simultaneously measuring total cellular DNA and incorporated nucleoside analog. The method entails altering the cellular DNA of cells grown in the presence of a nucleoside analog so that single stranded and double stranded portions are present. Separate stains are used against the two portions. An immunochemical stain is used against the single stranded portion to provide a measure of incorporated nucleoside analog, and a double strand DNA-specific stain is used against the double stranded portion to simultaneously provide a measure of total cellular DNA. The method permits rapid flow cytometric analysis of cell populations, rapid identification of cycling and noncycling subpopulations, and determination of the efficacy of S phase cytotoxic anticancer agents.

  15. Method for construction of normalized cDNA libraries

    DOE Patents [OSTI]

    Soares, Marcelo B. (New York, NY); Efstratiadis, Argiris (Englewood, NJ)

    1998-01-01

    This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3' noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to appropriate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library. This invention also provides normalized cDNA libraries generated by the above-described method and uses of the generated libraries.

  16. Photoelectromechanical synthesis of low-cost DNA microarrays

    E-Print Network [OSTI]

    Chow, Brian, 1978-

    2008-01-01

    Recent advances in de novo gene synthesis, library construction, and genomic selection for target sequencing using DNA from custom microarrays have demonstrated that microarrays can effectively be used as the world's ...

  17. DNA Damage by Fasicularin Sanjay Dutta, Hideki Abe, Sakae Aoyagi, Chihiro Kibayashi, and Kent S. Gates*,

    E-Print Network [OSTI]

    Gates, Kent. S.

    DNA Damage by Fasicularin Sanjay Dutta, Hideki Abe, Sakae Aoyagi, Chihiro Kibayashi, and Kent S that a DNA repair- deficient cell line is hypersensitive to fasicularin suggest that an ability to damage DNA of DNA damage. For example, oxidatiVe damage at guanine residues can also generate base-labile lesions.13

  18. DNA BARCODING Tissue-direct PCR, a rapid and extraction-free method

    E-Print Network [OSTI]

    DNA BARCODING Tissue-direct PCR, a rapid and extraction-free method for barcoding of ferns F-W. LI gametophytes and young sporophytes often provide too little material for DNA extraction and are particularly. in press). DNA extraction has always been the first step in the DNA barcoding process. However, in practice

  19. Structural and torsional properties of the RAD51-dsDNA nucleoprotein filament

    E-Print Network [OSTI]

    Dekker, Nynke

    Structural and torsional properties of the RAD51-dsDNA nucleoprotein filament Mina Lee1 , Jan DNA, which induces changes in DNA structure, results in the formation of a nucleoprotein filament-dsDNA filaments. Our measurements use two recently developed magnetic tweezers assays, freely

  20. NEAFS Y-mtDNA Workshop (Butler and Coble) November 1, 2006

    E-Print Network [OSTI]

    Human Genome The Human Genome Nuclear DNA 3 billion bp High Power Of Discrimination Mitochondrial DNA 16 11 12 13 14 15 16 17 18 19 20 21 22 X Y Sex- chromosomes Autosomes 3.2 billion bp Nuclear DNA), and use a genetic code for amino acids different that the nuclear DNA. · New mitochondria are formed