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1

Lightning Dock Geothermal Facility | Open Energy Information  

Open Energy Info (EERE)

Geothermal Facility Geothermal Facility Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Lightning Dock Geothermal Facility General Information Name Lightning Dock Geothermal Facility Facility Lightning Dock Sector Geothermal energy Location Information Location Fallon, New Mexico Coordinates 35.115059°, -106.604598° Loading map... {"minzoom":false,"mappingservice":"googlemaps3","type":"ROADMAP","zoom":14,"types":["ROADMAP","SATELLITE","HYBRID","TERRAIN"],"geoservice":"google","maxzoom":false,"width":"600px","height":"350px","centre":false,"title":"","label":"","icon":"","visitedicon":"","lines":[],"polygons":[],"circles":[],"rectangles":[],"copycoords":false,"static":false,"wmsoverlay":"","layers":[],"controls":["pan","zoom","type","scale","streetview"],"zoomstyle":"DEFAULT","typestyle":"DEFAULT","autoinfowindows":false,"kml":[],"gkml":[],"fusiontables":[],"resizable":false,"tilt":0,"kmlrezoom":false,"poi":true,"imageoverlays":[],"markercluster":false,"searchmarkers":"","locations":[{"text":"","title":"","link":null,"lat":35.115059,"lon":-106.604598,"alt":0,"address":"","icon":"","group":"","inlineLabel":"","visitedicon":""}]}

2

Lightning Dock Geothermal Area | Open Energy Information  

Open Energy Info (EERE)

Lightning Dock Geothermal Area Lightning Dock Geothermal Area (Redirected from Lightning Dock Area) Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Geothermal Resource Area: Lightning Dock Geothermal Area Contents 1 Area Overview 2 History and Infrastructure 3 Regulatory and Environmental Issues 4 Exploration History 5 Well Field Description 6 Geology of the Area 7 Heat Source 8 Geofluid Geochemistry 9 NEPA-Related Analyses (6) 10 Exploration Activities (25) 11 References Loading map... {"minzoom":false,"mappingservice":"googlemaps3","type":"TERRAIN","zoom":6,"types":["ROADMAP","SATELLITE","HYBRID","TERRAIN"],"geoservice":"google","maxzoom":false,"width":"500px","height":"300px","centre":false,"title":"","label":"","icon":"","visitedicon":"","lines":[],"polygons":[],"circles":[],"rectangles":[],"copycoords":false,"static":false,"wmsoverlay":"","layers":[],"controls":["pan","zoom","type","scale","streetview"],"zoomstyle":"DEFAULT","typestyle":"DEFAULT","autoinfowindows":false,"kml":[],"gkml":[],"fusiontables":[],"resizable":false,"tilt":0,"kmlrezoom":false,"poi":true,"imageoverlays":[],"markercluster":false,"searchmarkers":"","locations":[{"text":"","title":"","link":null,"lat":32.14833333,"lon":-108.8316667,"alt":0,"address":"","icon":"","group":"","inlineLabel":"","visitedicon":""}]}

3

Lightning Dock Geothermal Area | Open Energy Information  

Open Energy Info (EERE)

Lightning Dock Geothermal Area Lightning Dock Geothermal Area Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Geothermal Resource Area: Lightning Dock Geothermal Area Contents 1 Area Overview 2 History and Infrastructure 3 Regulatory and Environmental Issues 4 Exploration History 5 Well Field Description 6 Geology of the Area 7 Heat Source 8 Geofluid Geochemistry 9 NEPA-Related Analyses (6) 10 Exploration Activities (25) 11 References Loading map... {"minzoom":false,"mappingservice":"googlemaps3","type":"TERRAIN","zoom":6,"types":["ROADMAP","SATELLITE","HYBRID","TERRAIN"],"geoservice":"google","maxzoom":false,"width":"500px","height":"300px","centre":false,"title":"","label":"","icon":"","visitedicon":"","lines":[],"polygons":[],"circles":[],"rectangles":[],"copycoords":false,"static":false,"wmsoverlay":"","layers":[],"controls":["pan","zoom","type","scale","streetview"],"zoomstyle":"DEFAULT","typestyle":"DEFAULT","autoinfowindows":false,"kml":[],"gkml":[],"fusiontables":[],"resizable":false,"tilt":0,"kmlrezoom":false,"poi":true,"imageoverlays":[],"markercluster":false,"searchmarkers":"","locations":[{"text":"","title":"","link":null,"lat":32.14833333,"lon":-108.8316667,"alt":0,"address":"","icon":"","group":"","inlineLabel":"","visitedicon":""}]}

4

Lightning Dock II Geothermal Project | Open Energy Information  

Open Energy Info (EERE)

Lightning Dock II Geothermal Project Lightning Dock II Geothermal Project Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Development Project: Lightning Dock II Geothermal Project Project Location Information Coordinates 32.346944444444°, -108.70722222222° Loading map... {"minzoom":false,"mappingservice":"googlemaps3","type":"ROADMAP","zoom":14,"types":["ROADMAP","SATELLITE","HYBRID","TERRAIN"],"geoservice":"google","maxzoom":false,"width":"600px","height":"350px","centre":false,"title":"","label":"","icon":"","visitedicon":"","lines":[],"polygons":[],"circles":[],"rectangles":[],"copycoords":false,"static":false,"wmsoverlay":"","layers":[],"controls":["pan","zoom","type","scale","streetview"],"zoomstyle":"DEFAULT","typestyle":"DEFAULT","autoinfowindows":false,"kml":[],"gkml":[],"fusiontables":[],"resizable":false,"tilt":0,"kmlrezoom":false,"poi":true,"imageoverlays":[],"markercluster":false,"searchmarkers":"","locations":[{"text":"","title":"","link":null,"lat":32.346944444444,"lon":-108.70722222222,"alt":0,"address":"","icon":"","group":"","inlineLabel":"","visitedicon":""}]}

5

Lightning dock geothermal space heating project, Lightning Dock KGRA, New Mexico. Final report  

DOE Green Energy (OSTI)

The proposed project was to take the existing geothermal greenhouse and home heating systems, which consisted of pumping geothermal water and steam through passive steam heaters, and convert the systems to one using modern heat exchange units. It was proposed to complete the existing unfinished, re-inforced glass side wall, wood framed structure, as a nursery lath house, the purpose of which would be to use geothermal water in implementing university concepts on the advantages of bottom heat to establish hardy root systems in nursery and bedding plants. The use of this framework was abandoned in favor of erecting new structures for the proposed purpose. The final project of the proposal was the establishment of a drip irrigation system, to an area just west of the existing greenhouse and within feet of the geothermal well. Through this drip irrigation system geothermal water would be pumped, to prevent killing spring frosts. The purpose of this area of the proposal is to increase the potential use of existing geothermal waters of the Lightning Dock KGRA, in opening a new geothermal agri-industry which is economically feasible for the area and would be extremely energy efficient.

McCants, T.W.

1980-12-01T23:59:59.000Z

6

Water-Gas Samples At Lightning Dock Area (Norman & Moore, 2004...  

Open Energy Info (EERE)

Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Water-Gas Samples At Lightning Dock Area (Norman & Moore, 2004) Exploration Activity Details...

7

Surface Gas Sampling At Lightning Dock Area (Norman, Et Al., 2002) | Open  

Open Energy Info (EERE)

Surface Gas Sampling At Lightning Dock Area (Norman, Et Al., 2002) Surface Gas Sampling At Lightning Dock Area (Norman, Et Al., 2002) Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Surface Gas Sampling At Lightning Dock Area (Norman, Et Al., 2002) Exploration Activity Details Location Lightning Dock Area Exploration Technique Surface Gas Sampling Activity Date Usefulness not indicated DOE-funding Unknown Notes The Lightning Dock, Animas Valley, New Mexico geothermal area was discovered when a rancher found boiling water while drilling a shallow stock tank welt (Elston, Deal, et. al, 1983). There are no surface manifestations of present or past geothermal activity in the Animas Valley. Norman and Bernhart (1982) analyzed the gases in the discovery well and 15 stock tank wells nearby (Figure 1).

8

Thermal Gradient Holes At Lightning Dock Area (Cunniff & Bowers, 2005) |  

Open Energy Info (EERE)

Thermal Gradient Holes At Lightning Dock Area Thermal Gradient Holes At Lightning Dock Area (Cunniff & Bowers, 2005) Exploration Activity Details Location Lightning Dock Area Exploration Technique Thermal Gradient Holes Activity Date Usefulness not indicated DOE-funding Unknown Notes The two gradient holes were sited on federal geothermal leases owned by Lightning Dock Geothermal, Inc. and both were drilled into lakebed sediments some distance from the intense shallow geothermal anomaly located in the eastern half of Section 7, Township 25 South, Range 19 West. References Roy A. Cunniff, Roger L. Bowers (2005) Final Technical Report, Geothermal Resource Evaluation And Definitioni (Gred) Program-Phases I, Ii, And Iii For The Animas Valley, Nm Geothermal Resource Retrieved from "http://en.openei.org/w/index.php?title=Thermal_Gradient_Holes_At_Lightning_Dock_Area_(Cunniff_%26_Bowers,_2005)&oldid=387460"

9

Lightning Dock I Geothermal Project | Open Energy Information  

Open Energy Info (EERE)

I Geothermal Project I Geothermal Project Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Development Project: Lightning Dock I Geothermal Project Project Location Information Coordinates 32.346944444444°, -108.70722222222° Loading map... {"minzoom":false,"mappingservice":"googlemaps3","type":"ROADMAP","zoom":14,"types":["ROADMAP","SATELLITE","HYBRID","TERRAIN"],"geoservice":"google","maxzoom":false,"width":"600px","height":"350px","centre":false,"title":"","label":"","icon":"","visitedicon":"","lines":[],"polygons":[],"circles":[],"rectangles":[],"copycoords":false,"static":false,"wmsoverlay":"","layers":[],"controls":["pan","zoom","type","scale","streetview"],"zoomstyle":"DEFAULT","typestyle":"DEFAULT","autoinfowindows":false,"kml":[],"gkml":[],"fusiontables":[],"resizable":false,"tilt":0,"kmlrezoom":false,"poi":true,"imageoverlays":[],"markercluster":false,"searchmarkers":"","locations":[{"text":"","title":"","link":null,"lat":32.346944444444,"lon":-108.70722222222,"alt":0,"address":"","icon":"","group":"","inlineLabel":"","visitedicon":""}]}

10

Surface Gas Sampling At Lightning Dock Area (Norman & Moore, 2004) | Open  

Open Energy Info (EERE)

source source History View New Pages Recent Changes All Special Pages Semantic Search/Querying Get Involved Help Apps Datasets Community Login | Sign Up Search Page Edit History Facebook icon Twitter icon » Surface Gas Sampling At Lightning Dock Area (Norman & Moore, 2004) Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Surface Gas Sampling At Lightning Dock Area (Norman & Moore, 2004) Exploration Activity Details Location Lightning Dock Area Exploration Technique Surface Gas Sampling Activity Date Usefulness useful DOE-funding Unknown References David I. Norman, Joseph Moore (2004) Gas Analysis Of Geothermal Fluid Inclusions- A New Technology For Geothermal Exploration Retrieved from "http://en.openei.org/w/index.php?title=Surface_Gas_Sampling_At_Lightning_Dock_Area_(Norman_%26_Moore,_2004)&oldid=689367"

11

Direct-Current Resistivity Survey At Lightning Dock Area (Cunniff & Bowers,  

Open Energy Info (EERE)

Direct-Current Resistivity Survey At Lightning Dock Area (Cunniff & Bowers, Direct-Current Resistivity Survey At Lightning Dock Area (Cunniff & Bowers, 2005) Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Direct-Current Resistivity Survey At Lightning Dock Area (Cunniff & Bowers, 2005) Exploration Activity Details Location Lightning Dock Area Exploration Technique Direct-Current Resistivity Survey Activity Date Usefulness not indicated DOE-funding Unknown Notes Two electrical resistivity survey lines were run in the project area: a southern east-west line along Caliche Road, and a northern east-west line in the south half Section 6, T25S, R19W (figure 5). The Caliche Road line is located south of the greenhouse complex and was run along the road, which was also used for a gravity traverse. The northern line, named

12

Flow Test At Lightning Dock Area (Cunniff & Bowers, 2005) | Open Energy  

Open Energy Info (EERE)

Flow Test At Lightning Dock Area (Cunniff & Bowers, 2005) Flow Test At Lightning Dock Area (Cunniff & Bowers, 2005) Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Flow Test At Lightning Dock Area (Cunniff & Bowers, 2005) Exploration Activity Details Location Lightning Dock Area Exploration Technique Flow Test Activity Date Usefulness useful DOE-funding Unknown Notes After the Welaco temperature survey was completed for TG52-7, preparations were completed for a controlled airlift test. This test was completed in the period from 19-20 September 2003 for some 23 hours. The well produced steady state flow of about 320-325 gpm at a wellhead temperature of 126.7degrees C (260degreesF). This production rate is equivalent to about 162,000 pounds per hour, with the production temperature producing usable

13

Direct-Current Resistivity At Lightning Dock Area (Cunniff & Bowers, 2005)  

Open Energy Info (EERE)

Lightning Dock Area (Cunniff & Bowers, 2005) Lightning Dock Area (Cunniff & Bowers, 2005) Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Direct-Current Resistivity At Lightning Dock Area (Cunniff & Bowers, 2005) Exploration Activity Details Location Lightning Dock Area Exploration Technique Direct-Current Resistivity Survey Activity Date Usefulness not indicated DOE-funding Unknown Notes Two electrical resistivity survey lines were run in the project area: a southern east-west line along Caliche Road, and a northern east-west line in the south half Section 6, T25S, R19W (figure 5). The Caliche Road line is located south of the greenhouse complex and was run along the road, which was also used for a gravity traverse. The northern line, named "Church Road,"+K846 was parallel to the east-west road and gravity

14

Observation Wells At Lightning Dock Area (Warpinski, Et Al., 2004) | Open  

Open Energy Info (EERE)

Lightning Dock Area (Warpinski, Et Al., 2004) Lightning Dock Area (Warpinski, Et Al., 2004) Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Observation Wells At Lightning Dock Area (Warpinski, Et Al., 2004) Exploration Activity Details Location Lightning Dock Area Exploration Technique Observation Wells Activity Date Usefulness not indicated DOE-funding Unknown Notes The awardee conducted seismic, gravity, resistivity, and airborne magnetic surveys, drilled temperature-gradient wells, and selected a location for a test well (52-7). The test well was drilled to a total depth of 770 m during 2003. Maximum temperatures approached 140degrees C and a short flow test suggested that a production well could be drilled to 600 m and produce economic volumes of 130-140degrees C fluid. A final assessment of the

15

Gas Flux Sampling At Lightning Dock Area (Cunniff & Bowers, 2005) | Open  

Open Energy Info (EERE)

Lightning Dock Area (Cunniff & Bowers, 2005) Lightning Dock Area (Cunniff & Bowers, 2005) Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Gas Flux Sampling At Lightning Dock Area (Cunniff & Bowers, 2005) Exploration Activity Details Location Lightning Dock Area Exploration Technique Gas Flux Sampling Activity Date Usefulness not useful DOE-funding Unknown Notes Based on all of the data, McLin concluded that there was little to no correlation between values for CO2 flux and known or postulated faults, and between the CO2 flux and the shallow thermal anomaly. Instead, the flux values appeared to depict a completely random pattern throughout the study area. Notably, absolute values for CO2 flux were elevated throughout the surveyed areas (McLin, 2004). A possible explanation not considered by

16

Ground Gravity Survey At Lightning Dock Area (Cunniff & Bowers, 2005) |  

Open Energy Info (EERE)

Ground Gravity Survey At Lightning Dock Area (Cunniff Ground Gravity Survey At Lightning Dock Area (Cunniff & Bowers, 2005) Exploration Activity Details Location Lightning Dock Area Exploration Technique Ground Gravity Survey Activity Date Usefulness useful DOE-funding Unknown Notes Two separate gravity surveys were conducted by LDG as part of this GRED Cooperative Agreement. The first survey was conducted in April 2001 and consisted of 77 stations in the north half of Section 7 and south half of Section 6, both sections being in Township 25 South, Range 19 West. A second and much larger survey was conducted in October 2001. This survey consisted of 227 new stations in nine linear traverses that covered more than one hundred (100) square kilometers centered on the known resource area in Section 7 (figure 3).

17

Thermal Ion Dispersion At Lightning Dock Area (Cunniff & Bowers, 2005) |  

Open Energy Info (EERE)

source source History View New Pages Recent Changes All Special Pages Semantic Search/Querying Get Involved Help Apps Datasets Community Login | Sign Up Search Page Edit History Facebook icon Twitter icon » Thermal Ion Dispersion At Lightning Dock Area (Cunniff & Bowers, 2005) Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Thermal Ion Dispersion At Lightning Dock Area (Cunniff & Bowers, 2005) Exploration Activity Details Location Lightning Dock Area Exploration Technique Thermal Ion Dispersion Activity Date Usefulness useful DOE-funding Unknown Notes Thermal Ion Dispersion (TID) is a method used by the precious-metals industry to determine the movement of hot, mineral-bearing waters through rocks, gravels, and soils. The survey involves collection of soil samples

18

Ground Gravity Survey At Lightning Dock Area (Warpinski, Et Al., 2002) |  

Open Energy Info (EERE)

source source History View New Pages Recent Changes All Special Pages Semantic Search/Querying Get Involved Help Apps Datasets Community Login | Sign Up Search Page Edit History Facebook icon Twitter icon » Ground Gravity Survey At Lightning Dock Area (Warpinski, Et Al., 2002) Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Ground Gravity Survey At Lightning Dock Area (Warpinski, Et Al., 2002) Exploration Activity Details Location Lightning Dock Area Exploration Technique Ground Gravity Survey Activity Date Usefulness not indicated DOE-funding Unknown Notes As a foundation for successful siting and drilling a deep test well, additional geophysical work has been completed including gravity, resistivity, and airborne magnetic surveys. Several new seismic profiles

19

Water-Gas Samples At Lightning Dock Area (Norman, Et Al., 2002) | Open  

Open Energy Info (EERE)

2002) 2002) Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Water-Gas Samples At Lightning Dock Area (Norman, Et Al., 2002) Exploration Activity Details Location Lightning Dock Area Exploration Technique Water-Gas Samples Activity Date Usefulness not indicated DOE-funding Unknown Notes The Lightning Dock, Animas Valley, New Mexico geothermal area was discovered when a rancher found boiling water while drilling a shallow stock tank welt (Elston, Deal, et. al, 1983). There are no surface manifestations of present or past geothermal activity in the Animas Valley. Norman and Bernhart (1982) analyzed the gases in the discovery well and 15 stock tank wells nearby (Figure 1). References David Norman, Nigel Blarney, Lynne Kurilovitch (2002) New

20

Reflection Survey At Lightning Dock Area (Cunniff & Bowers, 2005) | Open  

Open Energy Info (EERE)

Reflection Survey At Lightning Dock Area (Cunniff & Reflection Survey At Lightning Dock Area (Cunniff & Bowers, 2005) Exploration Activity Details Location Lightning Dock Area Exploration Technique Reflection Survey Activity Date Usefulness useful DOE-funding Unknown Notes After reviewing bids from six firms, LDG contracted with Bird Geophysical Services ("Bird") to conduct a test to determine if relatively small, spring-assisted, drop weights could be used to successfully acquire deep reflections. This test showed that the contractor could produce usable data to depths of more than 1,500 ms two-way travel time. (For a given velocity model, this two-way travel time is equivalent to several kilometers of depth penetration.) Subsequently, LDG used Bird's services to acquire new traverses totaling about 27.6 km (17.2 mi.) along roads leading through the

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


21

Thermal Gradient Holes At Lightning Dock Area (Warpinski, Et Al., 2004) |  

Open Energy Info (EERE)

Thermal Gradient Holes At Lightning Dock Area Thermal Gradient Holes At Lightning Dock Area (Warpinski, Et Al., 2004) Exploration Activity Details Location Lightning Dock Area Exploration Technique Thermal Gradient Holes Activity Date Usefulness not indicated DOE-funding Unknown Notes The awardee conducted seismic, gravity, resistivity, and airborne magnetic surveys, drilled temperature-gradient wells, and selected a location for a test well (52-7). The test well was drilled to a total depth of 770 m during 2003. Maximum temperatures approached 140degrees C and a short flow test suggested that a production well could be drilled to 600 m and produce economic volumes of 130-140degrees C fluid. A final assessment of the resource is currently being performed. References N. R. Warpinski, A. R. Sattler, R. Fortuna, D. A. Sanchez, J.

22

Stepout-Deepening Wells At Lightning Dock Area (Warpinski, Et Al., 2004) |  

Open Energy Info (EERE)

Well Deepening At Lightning Dock Area (Warpinski, Et Well Deepening At Lightning Dock Area (Warpinski, Et Al., 2004) Exploration Activity Details Location Lightning Dock Area Exploration Technique Well Deepening Activity Date Usefulness not indicated DOE-funding Unknown Notes The objective of this project is to access, test, and confirm the deeper resource by deepening an existing shallow well at the site to penetrate the deep reservoir. AmeriCulture deepened the existing well (EGS-1) in 2003 by coring from 277 m to 640 m, essentially through the entire Tertiary volcanic interval. The maximum recorded temperature after stabilization was about 115degrees C. Evaluation of the well productivity will occur in 2004. References N. R. Warpinski, A. R. Sattler, R. Fortuna, D. A. Sanchez, J. Nathwani (2004) Geothermal Resource Exploration And Definition Projects

23

Direct-Current Resistivity Survey At Lightning Dock Area (Warpinski, Et  

Open Energy Info (EERE)

Et Et Al., 2002) Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Direct-Current Resistivity Survey At Lightning Dock Area (Warpinski, Et Al., 2002) Exploration Activity Details Location Lightning Dock Area Exploration Technique Direct-Current Resistivity Survey Activity Date Usefulness not indicated DOE-funding Unknown Notes As a foundation for successful siting and drilling a deep test well, additional geophysical work has been completed including gravity, resistivity, and airborne magnetic surveys. Several new seismic profiles are planned to provide more focused siting and drilling plans. These new geophysical surveys are being integrated into the combined thermal, hydrologic, and subsurface stratigraphic information data sets to provide a

24

Aeromagnetic Survey At Lightning Dock Area (Warpinski, Et Al., 2002) | Open  

Open Energy Info (EERE)

2) 2) Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Aeromagnetic Survey At Lightning Dock Area (Warpinski, Et Al., 2002) Exploration Activity Details Location Lightning Dock Area Exploration Technique Aeromagnetic Survey Activity Date Usefulness not indicated DOE-funding Unknown Notes As a foundation for successful siting and drilling a deep test well, additional geophysical work has been completed including gravity, resistivity, and airborne magnetic surveys. Several new seismic profiles are planned to provide more focused siting and drilling plans. These new geophysical surveys are being integrated into the combined thermal, hydrologic, and subsurface stratigraphic information data sets to provide a comprehensive integrated geothermal model. From all of this information,

25

Direct-Current Resistivity At Lightning Dock Area (Warpinski, Et Al., 2002)  

Open Energy Info (EERE)

2) 2) Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Direct-Current Resistivity At Lightning Dock Area (Warpinski, Et Al., 2002) Exploration Activity Details Location Lightning Dock Area Exploration Technique Direct-Current Resistivity Survey Activity Date Usefulness not indicated DOE-funding Unknown Notes As a foundation for successful siting and drilling a deep test well, additional geophysical work has been completed including gravity, resistivity, and airborne magnetic surveys. Several new seismic profiles are planned to provide more focused siting and drilling plans. These new geophysical surveys are being integrated into the combined thermal, hydrologic, and subsurface stratigraphic information data sets to provide a

26

Aeromagnetic Survey At Lightning Dock Area (Cunniff & Bowers, 2005) | Open  

Open Energy Info (EERE)

Cunniff & Bowers, 2005) Cunniff & Bowers, 2005) Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Aeromagnetic Survey At Lightning Dock Area (Cunniff & Bowers, 2005) Exploration Activity Details Location Lightning Dock Area Exploration Technique Aeromagnetic Survey Activity Date Usefulness useful DOE-funding Unknown Notes In October 2001, TerraCon, Inc. (2001) of Arlington, Texas conducted the highresolution aeromagnetic survey that was designed to explore the known, shallow geothermal resource and surrounding area. Shallow-subsurface Tertiary volcanic rocks were used as a magnetic basis for mapping structures References Roy A. Cunniff, Roger L. Bowers (2005) Final Technical Report, Geothermal Resource Evaluation And Definitioni (Gred) Program-Phases I, Ii,

27

Geology and geothermal waters of Lightning Dock region, Animas Valley and Pyramid Mountains, Hidalgo County, New Mexico  

DOE Green Energy (OSTI)

This circular covers the geology of the Pyramid Peak, Swallow Fork Peak, Table Top Mountain, and South Pyramid Peak 7-1/2-min quadrangles, which include the Lightning Dock KGRA. Hot wells (70 to 115.5/sup 0/C) seem to be structurally controlled by intersections of the ring-fracture zone of an Oligocene ash-flow tuff cauldron (Muir cauldron), a Miocene-to-Holocene north-trending basin-and-range fault (Animas Valley fault), and a northeast-trending lineament that appears to control anomalously heated underground waters and Pliocene-Pleistocene basalt cones in the San Bernardino, San Simon, and Animas Valleys. The Muir cauldron, approximately 20 km in diameter, collapsed in two stages, each associated with the eruption of a rhyolite ash-flow-tuff sheet and of ring-fracture domes. Most of the hydrothermal alteration of the Lightning Dock KGRA is related to the first stage of eruption and collapse, not to the modern geothermal system. Contrary to previous reports, no silicic volcanic rocks younger than basin-and-range faulting are known; unconformities beneath rhyolite ring-fracture domes are caused by Oligocene caldera collapse, not by basin-and-range faulting. The Animas Valley is the site of widespread post-20 My travertine deposits and near-surface veins of calcite, fluorite, and/or psilomelane, controlled by north- or northwest-trending basin-and-range faults. The fluoride-bearing waters of the Lightning Dock KGRA may be a late stage of this hydrothermal activity. Distribution of Pliocene-Pleistocene basalt suggests that deep-seated basalt near the solids may be the ultimate heat source.

Elston, W.E.; Deal, E.G.; Logsdon, M.J.

1983-01-01T23:59:59.000Z

28

Lightning  

NLE Websites -- All DOE Office Websites (Extended Search)

Lightning Lightning Nature Bulletin No. 458-A May 20, 1972 Forest Preserve District of Cook County George W. Dunne, President Roland F. Eisenbeis, Supt. of Conservation LIGHTNING Because we are so rapt up with our modern inventions, we forget that primitive man, ages before the beginnings of civilization, made one of the greatest discoveries of all time -- the use of fire. However, for thousands of years before he learned to kindle his own fires by friction or by striking sparks from flint, he snatched flaming firebrands from forest fires started by lightning. These he carried away and carefully fed with dry wood, year in and year out, to warm his cave, frighten away wild beasts and cook his food. Around the world, in every year, countless thousands of fires are started by lightning.

29

DOI-BLM-NM-L000-2012-0111-DNA | Open Energy Information  

Open Energy Info (EERE)

NM-L000-2012-0111-DNA NM-L000-2012-0111-DNA Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home NEPA Document Collection for: DOI-BLM-NM-L000-2012-0111-DNA DNA at Lightning Dock Geothermal Area for Geothermal/Exploration, {{{NEPA_Name}}} General NEPA Document Info Energy Sector Geothermal energy Environmental Analysis Type DNA Applicant Lightning Dock Geothermal Inc Geothermal Area Lightning Dock Geothermal Area Project Location New Mexico Project Phase Geothermal/Exploration Techniques Drilling Techniques Time Frame (days) Participating Agencies Lead Agency BLM Funding Agency none provided Managing District Office BLM Las Cruces District Office Managing Field Office none provided Funding Agencies none provided Surface Manager none provided Mineral Manager BLM Selected Dates

30

DOI-BLM-NM-L000-2012-0020-DNA | Open Energy Information  

Open Energy Info (EERE)

20-DNA 20-DNA Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home NEPA Document Collection for: DOI-BLM-NM-L000-2012-0020-DNA DNA at Lightning Dock Geothermal Area for Geothermal/Exploration {{{NEPA_Name}}} General NEPA Document Info Energy Sector Geothermal energy Environmental Analysis Type DNA Applicant Lightning Dock Geothermal Inc Geothermal Area Lightning Dock Geothermal Area Project Location New Mexico Project Phase Geothermal/Exploration Techniques Time Frame (days) Participating Agencies Lead Agency BLM Funding Agency none provided Managing District Office BLM Las Cruces District Office Managing Field Office none provided Funding Agencies none provided Surface Manager none provided Mineral Manager BLM Selected Dates Application Document Type Sundry Notice

31

DOI-BLM-NM-L000-2012-0218-DNA | Open Energy Information  

Open Energy Info (EERE)

DOI-BLM-NM-L000-2012-0218-DNA DOI-BLM-NM-L000-2012-0218-DNA Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home NEPA Document Collection for: DOI-BLM-NM-L000-2012-0218-DNA DNA at Lightning Dock Geothermal Area for Geothermal/Exploration {{{NEPA_Name}}} General NEPA Document Info Energy Sector Geothermal energy Environmental Analysis Type DNA Applicant Lightning Dock Geothermal Inc Geothermal Area Lightning Dock Geothermal Area Project Location New Mexico Project Phase Geothermal/Exploration Techniques Well Testing Techniques Time Frame (days) Participating Agencies Lead Agency BLM Funding Agency none provided Managing District Office BLM Las Cruces District Office Managing Field Office none provided Funding Agencies none provided Surface Manager none provided Mineral Manager BLM

32

DOI-BLM-NM-L000-2012-0042-DNA | Open Energy Information  

Open Energy Info (EERE)

2-DNA 2-DNA Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home NEPA Document Collection for: DOI-BLM-NM-L000-2012-0042-DNA DNA at Lightning Dock Geothermal Area for Geothermal/Exploration {{{NEPA_Name}}} General NEPA Document Info Environmental Analysis Type DNA Applicant Lightning Dock Geothermal Inc Geothermal Area Lightning Dock Geothermal Area Project Location New Mexico Project Phase Geothermal/Exploration Techniques Time Frame (days) Participating Agencies Lead Agency BLM Funding Agency none provided Managing District Office BLM Las Cruces District Office Managing Field Office none provided Funding Agencies none provided Surface Manager none provided Mineral Manager BLM Selected Dates Application Document Type Sundry Notice Relevant Numbers Lead Agency

33

DOI-BLM-NM-L000-2012-0200-DNA | Open Energy Information  

Open Energy Info (EERE)

DOI-BLM-NM-L000-2012-0200-DNA DOI-BLM-NM-L000-2012-0200-DNA Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home NEPA Document Collection for: DOI-BLM-NM-L000-2012-0200-DNA DNA at Lightning Dock Geothermal Area for Geothermal/Well Field, {{{NEPA_Name}}} General NEPA Document Info Energy Sector Geothermal energy Environmental Analysis Type DNA Applicant Lightning Dock Geothermal Inc Geothermal Area Lightning Dock Geothermal Area Project Location New Mexico Project Phase Geothermal/Well Field Techniques Drilling Techniques Time Frame (days) Participating Agencies Lead Agency BLM Funding Agency none provided Managing District Office BLM Las Cruces District Office Managing Field Office none provided Funding Agencies none provided Surface Manager none provided Mineral Manager BLM

34

DNA | Open Energy Information  

Open Energy Info (EERE)

DNA DNA Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home NEPA Casual Use Determination of NEPA Adequacy Categorical Exclusion Environmental Assessment Environmental Impact Statements Print PDF NEPA-Related Analysis: Determination of NEPA Adequacy (DNA) General Document Collections (28) Documents Regulatory Roadmap NEPA-Related Analysis: Determination of NEPA Adequacy and Land Use Plan Conformance (DNA) placeholder. This query has been included to allow you to use the black arrows in the table header cells to sort the table data. Document # Serial Number Applicant Lead Agency District Office Field Office Development Phase(s) Techniques DOI-BLM-NM-L000-2012-0020-DNA Lightning Dock Geothermal Inc BLM BLM Las Cruces District Office BLM Geothermal/Exploration

35

Nitrogen Fixation by Lightning  

Science Conference Proceedings (OSTI)

When some of the uncertainties associated with lightning are reviewed, it becomes difficult to support a large production of fixed nitrogen from the lightning shock wave.

G. A. Dawson

1980-01-01T23:59:59.000Z

36

Lightning Rod Improvement Studies  

Science Conference Proceedings (OSTI)

Although lightning rods have long been used to limit damage from lightning, there are currently no American standards for the shape and form of these devices. Following tradition, however, sharp-tipped Franklin rods are widely installed despite ...

C. B. Moore; William Rison; James Mathis; Graydon Aulich

2000-05-01T23:59:59.000Z

37

Laser Induced Lightning: Thunderstorm Lightning Diversion  

Science Conference Proceedings (OSTI)

Lightning causes extensive damage to industrial and commercial facilities and residences. In addition, utilities lose revenue from lighting caused outages, resulting in substantial losses. This project deals with the development of a laser based system to divert lightning from striking susceptible facilities, such as nuclear power plants, critical substations and lines, operation control centers, and customer process control plants.

1999-11-29T23:59:59.000Z

38

NATIONAL WEATHER SERVICE - Lightning Safety  

NLE Websites -- All DOE Office Websites (Extended Search)

WEATHER SERVICE - Lightning Safety Lightning: What You Need to Know * NO PLACE outside is safe when thunderstorms are in the area * If you hear thunder, lightning is close enough...

39

Pantex lightning study recommendations report  

Science Conference Proceedings (OSTI)

A brief history of lightning protection at Pantex nuclear explosive areas (NEAs) is given. An assessment of current Pantex lightning protection at NEAs is summarized. Recommendations for further improvements in lightning protection are described.

Chen, K.C.; Merewether, K.O.

1993-12-01T23:59:59.000Z

40

Direct-Current Resistivity Survey At Lightning Dock Area (Cunniff...  

Open Energy Info (EERE)

south of the greenhouse complex and was run along the road, which was also used for a gravity traverse. The northern line, named "Church Road,"+K846 was parallel to the east-west...

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


41

Direct-Current Resistivity Survey At Lightning Dock Area (Warpinski...  

Open Energy Info (EERE)

drilling a deep test well, additional geophysical work has been completed including gravity, resistivity, and airborne magnetic surveys. Several new seismic profiles are planned...

42

Direct-Current Resistivity At Lightning Dock Area (Warpinski...  

Open Energy Info (EERE)

drilling a deep test well, additional geophysical work has been completed including gravity, resistivity, and airborne magnetic surveys. Several new seismic profiles are planned...

43

Direct-Current Resistivity At Lightning Dock Area (Cunniff &...  

Open Energy Info (EERE)

south of the greenhouse complex and was run along the road, which was also used for a gravity traverse. The northern line, named "Church Road,"+K846 was parallel to the east-west...

44

Ground Gravity Survey At Lightning Dock Area (Warpinski, Et Al...  

Open Energy Info (EERE)

not indicated DOE-funding Unknown Notes Update to Warpinski, et al., 2002 References N. R. Warpinski, A. R. Sattler, R. Fortuna, D. A. Sanchez, J. Nathwani (2004) Geothermal...

45

Aeromagnetic Survey At Lightning Dock Area (Warpinski, Et Al...  

Open Energy Info (EERE)

not indicated DOE-funding Unknown Notes Update to Warpinski, et al., 2002 References N. R. Warpinski, A. R. Sattler, R. Fortuna, D. A. Sanchez, J. Nathwani (2004) Geothermal...

46

TOGA COARE: Oceanic Lightning  

Science Conference Proceedings (OSTI)

A lightning detection network composed of three direction finders was installed in the western Pacific during TOGA COARE. The results are reported from one direction finder, at Kavieng, Papua New Guinea, for the months of January and February ...

Christopher Lucas; Richard E. Orville

1996-09-01T23:59:59.000Z

47

Lightning Scaling Relations Revisited  

Science Conference Proceedings (OSTI)

Scaling relations connecting storm electrical generator power (and hence lightning flash rate) to charge transport velocity and storm geometry were originally posed by Vonnegut in the 1960s. These were later simplified to yield simple ...

Dennis J. Boccippio

2002-03-01T23:59:59.000Z

48

Lightning Safety and Large Stadiums  

Science Conference Proceedings (OSTI)

When lightning threatens an outdoor activity, it is usually postponed so that people may seek a safe location. When lightning threatens a large outdoor stadium, the game or event itself is usually postponed, but it is often difficult to ensure ...

Joel Gratz; Erik Noble

2006-09-01T23:59:59.000Z

49

SpaceTime Correlations of Lightning Distributions  

Science Conference Proceedings (OSTI)

Lightning location data are used to calculate the autocorrelation function of lightning distributions, which is a function of distances and time intervals between the single lightning events. This function is utilized for the characterization of ...

Ulli Finke

1999-08-01T23:59:59.000Z

50

Atmospheric Nitrogen Fixation by Lightning  

Science Conference Proceedings (OSTI)

The production Of nitrogen oxides (NO and NO2) by lightning flashes has been computed from a model of gaseous molecular reactions occurring as heated lightning-channel air cools by mixing with surrounding ambient air. The effect of ozone (O3) on ...

R. D. Hill; R. G. Rinker; H. Dale Wilson

1980-01-01T23:59:59.000Z

51

Quantum mechanical scoring for protein docking  

Science Conference Proceedings (OSTI)

We develop a docking protocol based on quantum mechanical/molecular mechanical calculations in which quantum mechanical energy is used as scoring. We test the protocol with three groups of examples with various binding site characteristics. The new docking method performs as well as or better than conventional docking methods in all three groups. In particular

Art E. Cho; Jae Yoon Chung

2009-01-01T23:59:59.000Z

52

A Lightning Data Assimilation Technique for Mesoscale Forecast Models  

Science Conference Proceedings (OSTI)

Lightning observations have been assimilated into a mesoscale model for improvement of forecast initial conditions. Data are used from the National Lightning Detection Network (cloud-to-ground lightning detection) and a Lightning Mapping Array (...

Edward R. Mansell; Conrad L. Ziegler; Donald R. MacGorman

2007-05-01T23:59:59.000Z

53

NATIONAL WEATHER SERVICE - Lightning Safety  

NLE Websites -- All DOE Office Websites (Extended Search)

NATIONAL WEATHER SERVICE - Lightning Safety Lightning: What You Need to Know * NO PLACE outside is safe when thunderstorms are in the area!! * If you hear thunder, lightning is close enough to strike you. * When you hear thunder, immediately move to safe shelter: a substantial building with electricity or plumbing or an enclosed, metal-topped vehicle with windows up. * Stay in safe shelter at least 30 minutes after you hear the last sound of thunder. Indoor Lightning Safety * Stay off corded phones, computers and other electrical equipment that put you in direct contact with electricity. * Avoid plumbing, including sinks, baths and faucets. * Stay away from windows and doors, and stay off porches. * Do not lie on concrete floors, and do not lean against concrete walls.

54

Performance Evaluation for a Lightning Location System Based on Observations of Artificially Triggered Lightning and Natural Lightning Flashes  

Science Conference Proceedings (OSTI)

Performance evaluation for the lightning location system (LLS) of the power grid in Guangdong Province, China, was conducted based on observation data of the triggered lightning flashes obtained in Conghua, Guangzhou, during 200711 and natural ...

Luwen Chen; Yijun Zhang; Weitao Lu; Dong Zheng; Yang Zhang; Shaodong Chen; Zhihui Huang

2012-12-01T23:59:59.000Z

55

Comparison of Lightning Data Provided by Three Lightning Location Networks in the United States  

Science Conference Proceedings (OSTI)

Three different lightning location systems (LLS), namely, the National Lightning Detection Network (NLDN), the U.S. Precision Lightning Network (USPLN), and the WeatherBug Total Lightning Network (WTLN), are offering lightning data on a wide scale in the United States. This report provides a comparison of those networks in terms of quality of the data provided to the end user. The report focuses on the cloud-to-ground (CG) lightning detection because this type of lightning is most critical for power util...

2011-12-13T23:59:59.000Z

56

A Lightning Parameterization for Numerical Cloud Models  

Science Conference Proceedings (OSTI)

A new lightning parameterization has been developed to enable cloud models to simulate the location and structure of individual lightning flashes more realistically. To do this, three aspects of previous parameterizations have been modified: 1) ...

Donald R. MacGorman; Jerry M. Straka; Conrad L. Ziegler

2001-03-01T23:59:59.000Z

57

Tropical Cyclone Lightning and Rapid Intensity Change  

Science Conference Proceedings (OSTI)

A large sample of Atlantic and eastern North Pacific tropical cyclone cases (200510) is used to investigate the relationships between lightning activity and intensity changes for storms over water. The lightning data are obtained from the ground-...

Mark DeMaria; Robert T. DeMaria; John A. Knaff; Debra Molenar

2012-06-01T23:59:59.000Z

58

Estimation of Convective Rainfall from Lightning Observations  

Science Conference Proceedings (OSTI)

The objective of this study is to develop a technique to use lightning observations for estimating convective rainfall. A framework for rainfall estimation is developed in which key elements are 1) the rainfalllightning ratio, that is, the ...

Alberto Tapia; James A. Smith; Michael Dixon

1998-11-01T23:59:59.000Z

59

Lightning in the Anvils of Supercell Thunderstorms  

Science Conference Proceedings (OSTI)

This study uses data from the Oklahoma Lightning Mapping Array (OK-LMA), the National Lightning Detection Network, and the Norman, Oklahoma (KOUN), prototype Weather Surveillance Radar-1988 Doppler (WSR-88D) radar to examine the evolution and ...

Stephanie A. Weiss; Donald R. MacGorman; Kristin M. Calhoun

2012-07-01T23:59:59.000Z

60

Lightning strokes can probe the ionosphere  

NLE Websites -- All DOE Office Websites (Extended Search)

Lightning Strokes Can Probe Ionosphere Lightning Strokes Can Probe Ionosphere Lightning strokes can probe the ionosphere Researchers have made measurements during thunderstorms to study the affect of lightning on the lower ionosphere and radiofrequency signals. April 11, 2013 Lightning. Credit: National Oceanic and Atmospheric Administration (NOAA) The team found that the electron density in the lower ionosphere decreased in response to lightning discharges. Thunderstorms, and the resulting partially ionized plasma of the ionosphere, can distort radio signals traveling to satellites important to communications, navigation or national security Los Alamos researchers and a collaborator have made measurements during thunderstorms to study the affect of lightning on the lower ionosphere and radiofrequency signals. This study supports one theory for how tropospheric

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


61

Lightning strokes can probe the ionosphere  

NLE Websites -- All DOE Office Websites (Extended Search)

Lightning Strokes Can Probe Ionosphere Lightning Strokes Can Probe Ionosphere Lightning strokes can probe the ionosphere Researchers have made measurements during thunderstorms to study the affect of lightning on the lower ionosphere and radiofrequency signals. April 11, 2013 Lightning. Credit: National Oceanic and Atmospheric Administration (NOAA) The team found that the electron density in the lower ionosphere decreased in response to lightning discharges. Thunderstorms, and the resulting partially ionized plasma of the ionosphere, can distort radio signals traveling to satellites important to communications, navigation or national security Los Alamos researchers and a collaborator have made measurements during thunderstorms to study the affect of lightning on the lower ionosphere and radiofrequency signals. This study supports one theory for how tropospheric

62

Computational methods for molecular docking  

Science Conference Proceedings (OSTI)

This tutorial was one of eight tutorials selected to be presented at the Third International Conference on Intelligent Systems for Molecular Biology which was held in the United Kingdom from July 16 to 19, 1995. Recently, it has been demonstrated that the knowledge of the three-dimensional structure of the protein can be used to derive new protein ligands with improved binding properties. This tutorial focuses on the following questions: What is its binding affinity toward a particular receptor? What are putative conformations of a ligand at the binding site? What are the similarities of different ligands in terms of their recognition capabilities? Where and in which orientation will a ligand bind to the active site? How is a new putative protein ligand selected? An overview is presented of the algorithms which are presently used to handle and predict protein-ligand interactions and to dock small molecule ligands into proteins.

Klebe, G. [BASF AG, Ludwigshafen (Germany); Lengauer, T.

1995-12-31T23:59:59.000Z

63

The Underreporting of Lightning injuries and Deaths in Colorado  

Science Conference Proceedings (OSTI)

Reliable statistics on lightning deaths and injuries are needed to raise the awareness of the community to the lightning threat and to educate the public to avoid situations vulnerable to lightning injuries. The principal source of information ...

Ral E. Lpez; Ronald L. Holle; Todd A. Heitkamp; Michael Boyson; Michael Cherington; Kenneth Langford

1993-11-01T23:59:59.000Z

64

Modeling Global Lightning Distributions in a General Circulation Model  

Science Conference Proceedings (OSTI)

A general circulation model (GCM) is used to model global lightning distributions and frequencies. Both total and cloud-to-ground lightning frequencies are modeled using parameterizations that relate the depth of convective clouds to lightning ...

Colin Price; David Rind

1994-08-01T23:59:59.000Z

65

Lightning Distribution and Eyewall Outbreaks in Tropical Cyclones during Landfall  

Science Conference Proceedings (OSTI)

Cloud-to-ground lightning data and storm intensity data (winds and central pressure) for 33 northwest Pacific tropical cyclones were used to analyze lightning distributions during the period of landfall in China. Lightning activities varied ...

Wenjuan Zhang; Yijun Zhang; Dong Zheng; Xiuji Zhou

2012-11-01T23:59:59.000Z

66

Lightning and Severe Weather: A Comparison between Total and Cloud-to-Ground Lightning Trends  

Science Conference Proceedings (OSTI)

Many studies over the past several decades have attempted to correlate trends in lightning (e.g., rates, polarity) to severe weather occurrence. These studies mainly used cloud-to-ground (CG) lightning information due to the ease of data ...

Christopher J. Schultz; Walter A. Petersen; Lawrence D. Carey

2011-10-01T23:59:59.000Z

67

Far-Field Power of Lightning Strokes as Measured by the World Wide Lightning Location Network  

Science Conference Proceedings (OSTI)

The World Wide Lightning Location Network (WWLLN) is a long-range network capable of locating lightning strokes in space and time. While able to locate lightning to within a few kilometers and tens of microseconds, the network currently does not ...

Michael L. Hutchins; Robert H. Holzworth; Craig J. Rodger; James B. Brundell

2012-08-01T23:59:59.000Z

68

Schools - CPU Damage Due to Lightning  

Science Conference Proceedings (OSTI)

This power quality (PQ) case study presents the investigation of the failure of certain equipment in the computer labs at a school which has been attributed to lightning.

2003-12-31T23:59:59.000Z

69

Schools - Electronic Equipment Damage Due to Lightning  

Science Conference Proceedings (OSTI)

This power quality (PQ) case study presents the investigation of damage to a school's phone equipment, security alarm, and network computer system during a lightning storm.

2003-12-31T23:59:59.000Z

70

LIGHTNING PROTECTION OF ROOF-MOUNTED SOLAR ...  

Science Conference Proceedings (OSTI)

Page 1. LIGHTNING PROTECTION OF ROOF-MOUNTED SOLAR CELLS ... Working paper developed for a NASA-sponsored study of solar cells ...

2013-05-17T23:59:59.000Z

71

Enhancement of CN Tower lightning current derivative signals using a modified power spectral subtraction method.  

E-Print Network (OSTI)

??Lightning current measurements are possible using instrumental tall structures or rocket-triggered lightning. The CN Tower has been a source of lightning current data for the (more)

Mehmud, Huma

2006-01-01T23:59:59.000Z

72

Atmospheric and Fuel Moisture Characteristics Associated with Lightning-Attributed Fires  

Science Conference Proceedings (OSTI)

A systematic examination is presented of the relationship between lightning occurrence and fires attributed to lightning ignitions. Lightning occurrence data are matched to a database of fires attributed to lightning ignition over southeastern ...

Andrew J. Dowdy; Graham A. Mills

2012-11-01T23:59:59.000Z

73

Docking automation related technology, Phase 2 report  

SciTech Connect

This report generalizes the progress for Phase II of the Docking Automated Related Technologies task component within the Modular Artillery Ammunition Delivery System (MAADS) technology demonstrator of the Future Armored Resupply Vehicle (FARV) project. This report also covers development activity at Oak Ridge National Laboratory (ORNL) during the period from January to July 1994.

Jatko, W.B.; Goddard, J.S.; Gleason, S.S.; Ferrell, R.K.

1995-04-01T23:59:59.000Z

74

New mechanism for lightning initiation  

Science Conference Proceedings (OSTI)

This is the final report of a three-year, Laboratory-Directed Research and Development (LDRD) project at the Los Alamos National Laboratory (LANL). To distinguish radio-frequency (rf) signals generated by lightning from the electromagnetic pulse produced by a nuclear explosion, it is necessary to understand the fundamental nature of thunderstorm discharges. The recent debate surrounding the origin of transionospheric pulse pairs (TIPPs) detected by the BLACKBEARD experiment aboard the ALEXIS satellite illustrates this point. We have argued that TIPP events could originate from the upward propagating discharges recently identified by optical images taken from the ground, from airplanes, and from the space shuttle. In addition, the Gamma Ray Observatory (GRO) measurements of x-ray bursts originating from thunderstorms are almost certainly associated with these upward propagating discharges. When taken together, these three measurements point directly to the runaway electron mechanism as the source of the upward discharges. The primary goal of this research effort was to identify the specific role played by the runaway-air-breakdown mechanism in the general area of thunderstorm electricity and in so doing develop lightning models that predict the optical, rf, and x-ray emissions that are observable from space.

Roussel-Dupre, R.; Buchwald, M.; Gurevich, A. [Lebedev Institute of Physical Sciences, Moscow (Russian Federation)] [and others

1996-10-01T23:59:59.000Z

75

Development of the National Lightning Detection Network  

Science Conference Proceedings (OSTI)

The development of the National Lightning Detection Network (NLDN) can be traced from the initial funding by the Electric Power Research Institute in June 1983. This support, when coupled with a small National Science Foundationsponsored ...

Richard E. Orville

2008-02-01T23:59:59.000Z

76

TELEX The Thunderstorm Electrification and Lightning Experiment  

Science Conference Proceedings (OSTI)

The field program of the Thunderstorm Electrification and Lightning Experiment (TELEX) took place in central Oklahoma, MayJune 2003 and 2004. It aimed to improve understanding of the interrelationships among microphysics, kinematics, ...

Donald R. MacGorman; W. David Rust; Conrad L. Ziegler; Edward R. Mansell; Terry J. Schuur; Michael I. Biggerstaff; Jerry M. Straka; Eric C. Bruning; Kristin M. Kuhlman; Nicole R. Lund; Clark Payne; Nicholas S. Biermann; William H. Beasley; Larry D. Carey; Paul R. Krehbiel; William Rison; Kenneth B. Eack

2008-07-01T23:59:59.000Z

77

Schools - Lightning Causing Electric Equipment Damage  

Science Conference Proceedings (OSTI)

This power quality (PQ) case study presents the investigation of damage to a school's electronic controls and integrated circuit boards within their fire alarm and clock/bell system when there are lightning storms.

2003-12-31T23:59:59.000Z

78

Regional Differences in Tropical Lightning Distributions  

Science Conference Proceedings (OSTI)

Observations from the National Aeronautics and Space Administration Optical Transient Detector (OTD) and Tropical Rainfall Measuring Mission (TRMM)-based Lightning Imaging Sensor (LIS) are analyzed for variability between land and ocean, various ...

Dennis J. Boccippio; Steven J. Goodman; Stan Heckman

2000-12-01T23:59:59.000Z

79

Lightning protection system for a wind turbine  

DOE Patents (OSTI)

In a wind turbine (104, 500, 704) having a plurality of blades (132, 404, 516, 744) and a blade rotor hub (120, 712), a lightning protection system (100, 504, 700) for conducting lightning strikes to any one of the blades and the region surrounding the blade hub along a path around the blade hub and critical components of the wind turbine, such as the generator (112, 716), gearbox (708) and main turbine bearings (176, 724).

Costin, Daniel P. (Chelsea, VT); Petter, Jeffrey K. (Williston, VT)

2008-05-27T23:59:59.000Z

80

Sizing of Overhead Ground Wires for Lightning  

Science Conference Proceedings (OSTI)

Overhead ground wires, or shield wires, are placed on transmission lines as air terminals for lightning strikes to shield the phase conductors from direct strikes. Although of short duration, lightning strikes to ground wires may result in significant damage. Utilities are increasingly using optical fiber ground wires (OPGWs), and severe damage to the OPGWs could result in failure of the communication link. There is renewed interest in the dimensioning and testing of ground wires for improved ...

2012-12-12T23:59:59.000Z

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


81

Lightning Arrestor Connectors Production Readiness  

SciTech Connect

The Lightning Arrestor Connector (LAC), part M, presented opportunities to improve the processes used to fabricate LACs. The A## LACs were the first production LACs produced at the KCP, after the product was transferred from Pinnellas. The new LAC relied on the lessons learned from the A## LACs; however, additional improvements were needed to meet the required budget, yield, and schedule requirements. Improvement projects completed since 2001 include Hermetic Connector Sealing Improvement, Contact Assembly molding Improvement, development of a second vendor for LAC shells, general process improvement, tooling improvement, reduction of the LAC production cycle time, and documention of the LAC granule fabrication process. This report summarizes the accomplishments achieved in improving the LAC Production Readiness.

Marten, Steve; Linder, Kim; Emmons, Jim; Gomez, Antonio; Hasam, Dawud; Maurer, Michelle

2008-10-20T23:59:59.000Z

82

A Review of Positive and Bipolar Lightning Discharges  

Science Conference Proceedings (OSTI)

Characteristics of lightning discharges that transport either positive charge or both positive and negative charges to the ground are reviewed. These are termed positive and bipolar lightning discharges, respectively. Different types of positive ...

V. A. Rakov

2003-06-01T23:59:59.000Z

83

Positive Cloud-to-Ground Lightning in Mesoscale Convective Systems  

Science Conference Proceedings (OSTI)

We have examined the characteristics of positive cloud-to-ground lightning flashes in Mesoscale Convective Systems observed during the Oklahoma-Kansas PRE-STORM project in 1985. Lightning frequencies and patterns of ground strike locations are ...

Steven A. Rutledge; Chungu Lu; Donald R. MacGorman

1990-09-01T23:59:59.000Z

84

Some Causes for Lightning Data Inaccuracies: The Case of Michigan  

Science Conference Proceedings (OSTI)

Based on records currently available, no complete knowledge exists of the number of casualties caused by lightning in the United States. The compilation of lightning casualty statistics is not a simple problem, and there are many unresolved ...

Kathleen M. Shearman; Carl F. Ojala

1999-09-01T23:59:59.000Z

85

A Radar Study of the Plasma and Geometry of Lightning  

Science Conference Proceedings (OSTI)

Radar measurements and model studies are combined to investigate the plasma condition and the physical structure of lightning in thunderclouds. The lightning radar target is inferred to be an arclike plasma whose temperature exceeds 5000 K, ...

Earle R. Williams; Spiros G. Geotis; A. B. Bhattacharya

1989-05-01T23:59:59.000Z

86

A CRITICAL REVIEW OF NONCONVENTIONAL APPROACHES TO LIGHTNING PROTECTION  

Science Conference Proceedings (OSTI)

The conventional technique for the lightning protection of structures is described in the U.S. National Standard, NFPA 780, and in many other national and international lightning protection standards. Two nonconventional techniques, known ...

M. A. Uman; V. A. Rakov

2002-12-01T23:59:59.000Z

87

Performance Characteristics of Distinct Lightning Detection Networks Covering Belgium  

Science Conference Proceedings (OSTI)

This study reports results from electric field measurements coupled to high-speed camera observations of cloud-to-ground lightning to test the performance of lightning location networks in terms of its detection efficiency and location accuracy. ...

Dieter R. Poelman; Wolfgang Schulz; Christian Vergeiner

2013-05-01T23:59:59.000Z

88

Optical Observations of Lightning from a High-Altitude Airplane  

Science Conference Proceedings (OSTI)

Lightning has been observed from above cloud top by using satellites, balloons, rockets, and high-altitude airplanes, each of which provides a unique perspective and holds the potential for gaining new understanding of lightning phenomena. During ...

H. J. Christian; S. J. Goodman

1987-12-01T23:59:59.000Z

89

A Reassessment of U.S. Lightning Mortality  

Science Conference Proceedings (OSTI)

Lightning is a unique weather hazard when compared to other perils such as tornadoes, flash floods, and hurricanes since lightning itself does not constitute a criterion for a severe event according to National Weather Service guidelines. Indeed, ...

Walker S. Ashley; Christopher W. Gilson

2009-10-01T23:59:59.000Z

90

Possible Aerosol Effects on Lightning Activity and Structure of Hurricanes  

Science Conference Proceedings (OSTI)

According to observations of hurricanes located relatively close to the land, intense and persistent lightning takes place within a 250300-km radius ring around the hurricane center, whereas the lightning activity in the eyewall takes place only ...

A. Khain; N. Cohen; B. Lynn; A. Pokrovsky

2008-12-01T23:59:59.000Z

91

A Linear Method for Analyzing Lightning Field Changes  

Science Conference Proceedings (OSTI)

A constrained, least-squares method for analyzing multiple-station measurements of lightning field changes (?Es) is introduced. Previous methods have attempted to fit the spatial pattern of lightning ?Es using nonlinear models, such as a point ...

William J. Koshak; E. Philip Krider

1994-02-01T23:59:59.000Z

92

High Voltage Laboratory Testing of Femtosecond Laser Lightning Diversion  

Science Conference Proceedings (OSTI)

Lightning strikes cost the electric power industry an estimated 1 billion dollars annually in damage and lost revenue. One possible way of protecting critical and susceptible facilities from lightning strikes is to use lasers to trigger and divert lightning along a predetermined path. This report describes laboratory research on the use of ultrashort UV pulses and near IR pulses to trigger high voltage discharge.

1998-12-09T23:59:59.000Z

93

Static electricity measurements for lightning warnings -an exploration  

E-Print Network (OSTI)

Static electricity measurements for lightning warnings - an exploration H. Bloemink De Bilt, 2013 | Internal report; IR 2013-01 #12;#12;Static electricity measurements for lightning warnings - an exploration Version 1.0 Date January 2013 Status Final #12;#12;Static electricity measurements for lightning warnings

Haak, Hein

94

DNA  

NLE Websites -- All DOE Office Websites (Extended Search)

directed directed assembly of nanoparticle linear structure for nanophotonics Baoquan Ding, a͒ Stefano Cabrini, b͒ Ronald N. Zuckermann, and Jeffrey Bokor Molecular Foundry, Lawrence Berkeley National Laboratory, 1 Cyclotron Rd., Berkeley, California 94720 ͑Received 17 June 2008; accepted 22 December 2008; published 2 February 2009͒ Assemblies of metal nanospheres have shown interesting properties for nanophotonics. Here the authors describe a method to use robust DNA multicrossover molecules to organize Au nanoparticles with different sizes to form well controlled linear chain structures with desired distance below 10 nm between the particles. Au particles with only one piece of DNA attached are purified individually. Three different sizes DNA-Au conjugates then hybridize with five other DNA strands to form the stiff triple crossover ͑TX͒ motif. The linkage position

95

A randomized global optimization method for protein-protein docking  

E-Print Network (OSTI)

Apr 10, 2003... set of constants found this way was then consistently used to dock several other complexes obtained from the Brookhaven Protein Database.

96

The response of aeroshells to lightning  

SciTech Connect

Electrical discharges from a lightning simulator were directed at Mk12 aeroshells. Buckling of the aluminum substrate was observed after some 100-kA shots, and severe damage consisting of tearing of the aluminum and the production of inward flying aluminum shrapnel was observed after some 200-kA peak-current shots. Some shots resulted in severe damage to both the aluminum and the carbon-phenolic ablative material. It is reasonable to conclude from the experimental results that a lightning stroke with very high-peak current could, by itself, produce an opening in an Mk12 aeroshell. Because the aeroshell is part of the nuclear explosive safety exclusion region for the Mk12/W62 nuclear weapon, an opening would significantly reduce the assured safety of the weapon. It is unlikely that the observed interaction between lightning and the aeroshells would have been predicted by any form of computer simulation.

Loescher, D.H. [Sandia National Labs., Albuquerque, NM (United States); Dinallo, M.A. [Quatro Corp., Albuquerque, NM (United States)

1994-06-01T23:59:59.000Z

97

Thunderstorm and Lightning Studies using the FORTE Optical Lightning System (FORTE/OLS)  

Science Conference Proceedings (OSTI)

Preliminary observations of simultaneous RF and optical emissions from lightning as seen by the FORTE spacecraft are presented. RF/optical pairs of waveforms are routinely collected both as individual lightning events and as sequences of events associated with cloud-to-ground (CG) and intra-cloud (IC) flashes. CG pulses can be distinguished from IC pulses based on the properties of the RF and optical waveforms, but mostly based on the associated RF spectrograms. The RF spectrograms are very similar to previous ground-based VHF observations of lightning and show signatures associated with return strokes, stepped and dart leaders, and attachment processes,. RF emissions are observed to precede the arrival of optical emissions at the satellite by a mean value of 280 microseconds. The dual phenomenology nature of these observations are discussed in terms of their ability to contribute to a satellite-based lightning monitoring mission.

Argo, P.; Franz, R.; Green, J.; Guillen, J.L.; Jacobson, A.R.; Kirkland, M.; Knox, S.; Spalding, R.; Suszcynsky, D.M.

1999-02-01T23:59:59.000Z

98

Cloud-to-Ground Lightning Observations from TOGA COARE: Selected Results and Lightning Location Algorithms  

Science Conference Proceedings (OSTI)

Recently, observations of electrified oceanic convection and associated cloud-to-ground (CG) lightning were obtained over the tropical western Pacific Ocean during TOGA COARE (Tropical Ocean Global Atmosphere Coupled Ocean-Atmosphere Response ...

Walter A. Petersen; Steven A. Rutledge; Richard E. Orville

1996-04-01T23:59:59.000Z

99

Number of lightning discharges causing damage to lightning arrester cables for aerial transmission lines in power systems  

SciTech Connect

Damage by lightning discharges to lightning arrester cables for 110-175 kV aerial transmission lines is analyzed using data from power systems on incidents with aerial transmission lines over a ten year operating period (1997-2006). It is found that failures of lightning arrester cables occur when a tensile force acts on a cable heated to the melting point by a lightning current. The lightning currents required to heat a cable to this extent are greater for larger cable cross sections. The probability that a lightning discharge will develop decreases as the amplitude of the lightning current increases, which greatly reduces the number of lightning discharges which damage TK-70 cables compared to TK-50 cables. In order to increase the reliability of lightning arrester cables for 110 kV aerial transmission lines, TK-70 cables should be used in place of TK-50 cables. The number of lightning discharges per year which damage lightning arrester cables is lowered when the density of aerial transmission lines is reduced within the territory of electrical power systems. An approximate relationship between these two parameters is obtained.

Nikiforov, E. P. [Electric Power Scientific Research Institute (VNIIE), branch of OAO 'NTTs Elektroenergetika' (Russian Federation)

2009-07-15T23:59:59.000Z

100

A Shape Descriptor for Fast Complementarity Matching in Molecular Docking  

Science Conference Proceedings (OSTI)

This paper presents a novel approach for fast rigid docking of proteins based on geometric complementarity. After extraction of the 3D molecular surface, a set of local surface patches is generated based on the local surface curvature. The shape complementarity ... Keywords: Protein docking, rigid body, geometric complementarity, shape impact descriptor.

Apostolos Axenopoulos; Petros Daras; Georgios Papadopoulos; Elias Houstis

2011-11-01T23:59:59.000Z

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


101

An East Coast Lightning Detection Network  

Science Conference Proceedings (OSTI)

A magnetic direction-finding network for the detection of lightning cloud-to-ground strikes has been installed, and it records most of the ground flashes from Maine to North Carolina and as far west as Ohio. This area includes or touches fourteen ...

Richard E. Orville; Ronald W. Henderson; Lance F. Bosart

1983-09-01T23:59:59.000Z

102

Mesoscale Observations of Lightning from Space Shuttle  

Science Conference Proceedings (OSTI)

Motion pictures have been taken at night by astronauts on the space shuttle showing lightning discharges that spread horizontally at speeds of 105 m s?1 for distances over 60 km. Tape recordings have been made of the accompanying optical pulses ...

B. Yonnegut; O. H. Vaughan Jr.; M. Brook; P. Krehbiel

1985-01-01T23:59:59.000Z

103

An Operational Comparison of Lightning Warning Systems  

Science Conference Proceedings (OSTI)

During the spring-summer of 1979, six lightning warning devices were evaluated in a side-by-side comparison study at three test sites. Stock commercial devices were selected based upon distinct concepts of operation. The devices tested included a ...

R. L. Johnson; D. E. Janota; J. E. Hay

1982-05-01T23:59:59.000Z

104

The Relationship between Total Cloud Lightning Behavior and Radar-Derived Thunderstorm Structure  

Science Conference Proceedings (OSTI)

Total lightning detection systems have been in development since the mid-1980s and deployed in several areas around the world. Previous studies on total lightning found intra- and intercloud lightning (IC) activity tends to fluctuate significantly ...

Eric Metzger; Wendell A. Nuss

2013-02-01T23:59:59.000Z

105

A Perfect Prognosis Scheme for Forecasting Warm-Season Lightning over Florida  

Science Conference Proceedings (OSTI)

This study develops and evaluates a statistical scheme for forecasting warm-season lightning over Florida. Four warm seasons of analysis data from the Rapid Update Cycle (RUC) and lightning data from the National Lightning Detection Network are ...

Phillip E. Shafer; Henry E. Fuelberg

2008-06-01T23:59:59.000Z

106

Lightning Flash Rates as an Indicator of Tropical Cyclone Genesis in the Eastern North Pacific  

Science Conference Proceedings (OSTI)

Lightning flashes in convective tropical clusters of the eastern North Pacific Ocean are detected by the Long-Range Lightning Detection Network and are analyzed for temporal patterns in electrical activity. The rates of lightning flash discharge ...

Lesley A. Leary; Elizabeth A. Ritchie

2009-10-01T23:59:59.000Z

107

Cloud-to-Ground Lightning Activity in the Contiguous United States from 1995 to 1999  

Science Conference Proceedings (OSTI)

The spatial and temporal distributions of cloud-to-ground lightning are examined over the contiguous United States from 1995 to 1999 using data from the National Lightning Detection Network. Annual flash density, annual lightning days, cumulative ...

Bard A. Zajac; Steven A. Rutledge

2001-05-01T23:59:59.000Z

108

Relationships among Lightning, Precipitation, and Hydrometeor Characteristics over the North Pacific Ocean  

Science Conference Proceedings (OSTI)

Lightning data from the Pacific Lightning Detection Network (PacNet) and Lightning Imaging Sensor (LIS) on the Tropical Rainfall Measuring Mission (TRMM) satellite were compared with TRMM precipitation radar products and latent heating and ...

Antti T. Pessi; Steven Businger

2009-04-01T23:59:59.000Z

109

Total Lightning Signatures of Thunderstorm Intensity over North Texas. Part II: Mesoscale Convective Systems  

Science Conference Proceedings (OSTI)

Total lightning data from the Lightning Detection and Ranging (LDAR II) research network in addition to cloud-to-ground flash data from the National Lightning Detection Network (NLDN) and data from the DallasFort Worth, Texas, Weather ...

Scott M. Steiger; Richard E. Orville; Lawrence D. Carey

2007-10-01T23:59:59.000Z

110

Total Lightning Signatures of Thunderstorm Intensity over North Texas. Part I: Supercells  

Science Conference Proceedings (OSTI)

It is shown that total lightning mapping, along with radar and National Lightning Detection Network (NLDN) cloud-to-ground lightning data, can be used to diagnose the severity of a thunderstorm. Analysis of supercells, some of which were tornadic,...

Scott M. Steiger; Richard E. Orville; Lawrence D. Carey

2007-10-01T23:59:59.000Z

111

GPU-accelerated high-accuracy molecular docking using guided differential evolution: real world applications  

Science Conference Proceedings (OSTI)

The objective in molecular docking is to determine the best binding mode of two molecules in silico. A common application of molecular docking is in drug discovery where a large number of ligands are docked against a protein to identify potential drug ... Keywords: GPGPU, differential evolution, molecular docking

Martin Simonsen; Christian N.S. Pedersen; Mikael H. Christensen; Ren Thomsen

2011-07-01T23:59:59.000Z

112

Electromagnetic field radiation model for lightning strokes to tall structures  

SciTech Connect

This paper describes observation and analysis of electromagnetic field radiation from lightning strokes to tall structures. Electromagnetic field waveforms and current waveforms of lightning strokes to the CN Tower have been simultaneously measured since 1991. A new calculation model of electromagnetic field radiation is proposed. The proposed model consists of the lightning current propagation and distribution model and the electromagnetic field radiation model. Electromagnetic fields calculated by the proposed model, based on the observed lightning current at the CN Tower, agree well with the observed fields at 2km north of the tower.

Motoyama, H. [CRIEPI, Tokyo (Japan); Janischewskyj, W.; Hussein, A.M. [Univ. of Toronto, Ontario (Canada); Chisholm, W.A. [Ontario Hydro Technologies, Toronto, Ontario (Canada); Chang, J.S. [McMaster Univ., Hamilton, Ontario (Canada); Rusan, R.

1996-07-01T23:59:59.000Z

113

Electromagnetic radiation from lightning return strokes to tall structures.  

E-Print Network (OSTI)

??The study of the interaction of lightning electromagnetic fields with electrical systems and the design of appropriate protection strategies are generally based on statistical distributions (more)

Pavanello, Davide

2007-01-01T23:59:59.000Z

114

Lightning Activities in the DOE-EPRI Turbine Verification Program  

DOE Green Energy (OSTI)

The US Department of Energy (DOE)-Electric Power Research Institute (EPRI) Turbine Verification Program (TVP) has played a vital role in improving the understanding of lightning protection for wind turbines. In response to concerns from host utilities, the TVP began a lightning protection project to study the impact of lightning activity at the 6.0-megawatt (MW) wind power facility in Ft. Davis, Texas. McNiff Light Industry (MLI) and Global Energy Concepts (GEC) established a broad observation and documentation effort to survey the lightning protection methodologies used and to evaluate the damage resulting from lightning activity at the turbines. The 6.05-MW wind power plant in Searsburg, Vermont, was inspected after a severe lightning storm caused damage to several turbines there. Zond, McNiff, and consultants from Lightning Technologies, Inc. conducted post-damage inspections at both sites to develop recommendations for improving lightning protection. Site operators implemented the recommended mitigation strategies, and the turbines were monitored to determine if the protection measures improved project operations. This paper summarizes the experience gained through TVP's lightning-related research, and provides a set of guidelines for wind turbine manufacturers, owners, and operators.

McCoy, T.; Rhoads, H.; Lisman, T. (Global Energy Concepts, LLC); McNiff, B. (McNiff Light Industry); Smith, B. (National Renewable Energy Laboratory)

2000-09-11T23:59:59.000Z

115

A genetic algorithm based method for docking flexible molecules  

SciTech Connect

The authors describe a computational method for docking flexible molecules into protein binding sites. The method uses a genetic algorithm (GA) to search the combined conformation/orientation space of the molecule to find low energy conformation. Several techniques are described that increase the efficiency of the basic search method. These include the use of several interacting GA subpopulations or niches; the use of a growing algorithm that initially docks only a small part of the molecule; and the use of gradient minimization during the search. To illustrate the method, they dock Cbz-GlyP-Leu-Leu (ZGLL) into thermolysin. This system was chosen because a well refined crystal structure is available and because another docking method had previously been tested on this system. Their method is able to find conformations that lie physically close to and in some cases lower in energy than the crystal conformation in reasonable periods of time on readily available hardware.

Judson, R.S. [Sandia National Labs., Livermore, CA (United States); Jaeger, E.P.; Treasurywala, A.M. [Sterling-Winthrop Inc., Collegeville, PA (United States)

1993-11-01T23:59:59.000Z

116

Grounding and Lightning Protection of Capacitor Controllers  

Science Conference Proceedings (OSTI)

This is the third report in EPRI's capacitor reliability study. It deals with capacitor controller grounding and lightning protection. Prior reports dealt with nuisance fuse operations, operating and construction practices, and use of distribution line capacitors for transmission voltage support. Simulations were performed to investigate various controller mounting locations and grounding configurations and their impact on the magnitude of surges reaching the controller. Once these aspects of capacitor c...

2005-03-08T23:59:59.000Z

117

Thermo-Bimorph Microciliaarrays For Small Spacecraft Docking  

E-Print Network (OSTI)

Microelectromechanical system (MEMS) technology promises to improve performance of future spacecraft components while reducing mass, cost, and manufacture time. Arrays of microcilia actuators offer a lightweight alternative to conventional docking systems for miniature satellites. Instead of mechanical guiding structures, such a system uses a surface tiled with MEMS actuators to guide the satellite to its docking site. This paper describes an experimental setup for precision docking of a "picosatellite" with the help of MEMS cilia arrays. Microgravity is simulated with an aluminum puck on an airtable. A series of experiments is performed to characterize the cilia, with the goal to understand the influence of normal force, picosat mass, docking velocity, cilia frequency, interface material, and actuation strategy ("gait") on the performance of the MEMS docking system. We demonstrate a 4 cm 2 cilia array capable of docking a 45 gram picosat with a 2 mm 2 contact area at micrometer precision. It is concluded that current MEMS cilia arrays are useful to position and align miniature satellites with up to several kg of mass.

Joel Reiter; Mason Terry; Karl F. Bhringer; John W. Suh

2000-01-01T23:59:59.000Z

118

Some Scientific Objectives of a Satellite-Borne Lightning Mapper  

Science Conference Proceedings (OSTI)

The Lightning Mapper Sensor is proposed as an instrument for use on a geosynchronous satellite in the late 1980s to monitor lightning activity continuously over broad areas of the earth. The system was suggested in response to a variety of needs ...

M. H. Davis; Marx Brook; Hugh Christian; Brian G. Heikes; Richard E. Orville; Chung G. Park; Raymond G. Roble; Bernard Vonnegut

1983-02-01T23:59:59.000Z

119

Radar Nowcasting of Total Lightning over the Kennedy Space Center  

Science Conference Proceedings (OSTI)

A long-term radar dataset over Melbourne, Florida, was matched with three-dimensional lightning data to optimize radar-derived predictors of total lightning over the Kennedy Space Center (KSC). Four years (200609) of summer (JuneAugust) daytime (...

Gregory N. Seroka; Richard E. Orville; Courtney Schumacher

2012-02-01T23:59:59.000Z

120

Overhead Transmission Line Lightning and Grounding Reference Book 2012  

Science Conference Proceedings (OSTI)

In North America and many other parts of the world, lightning remains the primary cause of transmission line outages, momentary interruptions, and reliability and maintenance problems. The lightning performance of transmission lines may be improved by applying overhead ground wires, providing a low impedance connection to ground, and selecting appropriate insulation levels for the phase conductors. The success of these measures is not always ...

2012-12-12T23:59:59.000Z

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


121

A Cloud-to-Ground Lightning Climatology for Romania  

Science Conference Proceedings (OSTI)

The first study of the characteristics of cloud-to-ground (CG) lightning in Romania, based on the data recorded by the Romanian National Lightning Detection Network (RNLDN), is presented. The data, more than 1.75 million CG flashes, covers the ...

Bogdan Antonescu; Sorin Burcea

2010-02-01T23:59:59.000Z

122

Field Measurement Studies on Shunt Coefficient of Lightning Shield Line  

Science Conference Proceedings (OSTI)

The shunt coefficient of lightning shield line is an important parameter, which needs to be considered when we are designing or reforming the substation grounding network, however, it is difficult to be calculated. This paper is to introduce a method ... Keywords: Short circuit current, Lightning shield line, Shunt coefficient, Shunt impedance

Dechao Li; Jinglu Li; Yuhuan Zhang

2008-10-01T23:59:59.000Z

123

Wind Turbine Lightning Protection Project: 1999-2001  

DOE Green Energy (OSTI)

A lightning protection research and support program was instituted by NREL to help minimize lightning damage to wind turbines in the United States. This paper provides the results of a field test program, an evaluation of protection on selected turbines, and a literature search as well as the dissemination of the accumulated information.

McNiff, B.

2002-05-01T23:59:59.000Z

124

Characterizing wind turbine system response to lightning activity  

DOE Green Energy (OSTI)

A lightning protection research program was instituted by National Renewable Energy Laboratory to minimize lightning damage to wind turbines and to further the understanding of effective damage mitigation techniques. To that end, a test program is under way to observe lightning activity, protection system response, and damage at a wind power plant in the Department of Energy (DOE) and Electric Power Research Institute (EPRI) Turbine Verification Program. The authors installed Lightning activated surveillance cameras along with a special storm tracking device to observe the activity in the wind plant area. They instrumented the turbines with lightning and ground current detection devices to log direct and indirect strike activity at each unit. They installed a surge monitor on the utility interface to track incoming activity from the transmission lines. Maintenance logs are used to verify damage and determine downtime and repair costs. Actual strikes to turbines were recorded on video and ancillary devices. The test setup and some results are discussed in this paper.

McNiff, B.; LaWhite, N. [McNiff Light Industry, Harborside, ME (United States); Muljadi, E. [National Renewable Energy Lab., Golden, CO (United States)

1998-07-01T23:59:59.000Z

125

GRED STUDIES AND DRILLING OF AMERICULTURE STATE 2, AMERICULTURE TILAPIA FARM LIGHTNING DOCK KGRA, ANIMAS VALLEY, NM  

Science Conference Proceedings (OSTI)

This report summarizes the GRED drilling operations in the AmeriCulture State 2 well with an overview of the preliminary geologic and geothermal findings, from drill cuttings, core, geophysical logs and water geochemical sampling.

Witcher, James

2006-08-01T23:59:59.000Z

126

Vertical Development of Lightning Activity Observed by the LDAR System: Lightning Bubbles  

Science Conference Proceedings (OSTI)

In some Florida thunderstorm cells, impulsive very high frequency (VHF) radiation from lightning channels begins abruptly in a layer that is typically 36 km in diameter, 13 km tall, and initially located just above the freezing level. In 208 ...

Tomoo Ushio; Stan J. Heckman; Hugh J. Christian; Zen-Ichiro Kawasaki

2003-02-01T23:59:59.000Z

127

Analysis of cloud-to-ground lightning in Hurricane Andrew  

E-Print Network (OSTI)

In August of 1992, Andrew joined a long list of tropical storms that have caused considerable damage and loss of life as they made landfall near a populated area. However, Andrew was unique in the sense that no previous landfalling tropical storm in the United States has provided such an excellent opportunity to study the cloud-to-ground (CG) lightning associated with this type of storm. While numerous thunderstorm systems, particularly the severe storms of the Great Plains, have been studied for lightning characteristics, the ability to conduct similar studies on hurricanes has been limited due to the small number which have occurred since the relatively new National Lightning Detection Network has been operational. 17,036 CG strikes over a 77 hour period were attributed to either the eyewall region or the primary spiral rainbands of Andrew. The overall distribution by polarity of the lightning was found to be 2.1% positive and 97.9% negative. As the storm was dissipating over land in Mississippi all lightning observed near the pressure center was positive. Throughout the lifetime of the storm, the negative first stroke peak current decreased while the positive first stroke peak current increased. The mean multiplicity of the negative flashes was 2.6, while the positive lightning had a mean value of 1.2. High-multiplicity flashes (negative CG flashes with 10 strokes or greater) tended to occur in four distinct groups and in time intervals of 19 to 23 hours apart in an area corresponding to the right-forward quadrant to right flank of the storm structure. Comparison of radar data to the lightning data shows that the deep convective regions of the outer rainbands were the areas with the most lightning. A lack of significant lightning observed in the eyewall region is consistent with previous research suggesting microphysical processes in this part of the storm are not favorable for charge separation.

George, William Randel

1995-01-01T23:59:59.000Z

128

Lightning and radar observations of hurricane Rita landfall  

SciTech Connect

Los Alamos National Laboratory (LANL) owns and operates an array of Very-Low Frequency (VLF) sensors that measure the Radio-Frequency (RF) waveforms emitted by Cloud-to-Ground (CG) and InCloud (IC) lightning. This array, the Los Alamos Sferic Array (LASA), has approximately 15 sensors concentrated in the Great Plains and Florida, which detect electric field changes in a bandwidth from 200 Hz to 500 kHz (Smith et al., 2002). Recently, LANL has begun development of a new dual-band RF sensor array that includes the Very-High Frequency (VHF) band as well as the VLF. Whereas VLF lightning emissions can be used to deduce physical parameters such as lightning type and peak current, VHF emissions can be used to perform precise 3d mapping of individual radiation sources, which can number in the thousands for a typical CG flash. These new dual-band sensors will be used to monitor lightning activity in hurricanes in an effort to better predict intensification cycles. Although the new LANL dual-band array is not yet operational, we have begun initial work utilizing both VLF and VHF lightning data to monitor hurricane evolution. In this paper, we present the temporal evolution of Rita's landfall using VLF and VHF lightning data, and also WSR-88D radar. At landfall, Rita's northern eyewall experienced strong updrafts and significant lightning activity that appear to mark a transition between oceanic hurricane dynamics and continental thunderstorm dynamics. In section 2, we give a brief overview of Hurricane Rita, including its development as a hurricane and its lightning history. In the following section, we present WSR-88D data of Rita's landfall, including reflectivity images and temporal variation. In section 4, we present both VHF and VLF lightning data, overplotted on radar reflectivity images. Finally, we discuss our observations, including a comparison to previous studies and a brief conclusion.

Henderson, Bradley G [Los Alamos National Laboratory; Suszcynsky, David M [Los Alamos National Laboratory; Hamlin, Timothy E [Los Alamos National Laboratory; Jeffery, C A [Los Alamos National Laboratory; Wiens, Kyle C [TEXAS TECH U.; Orville, R E [TEXAS A& M

2009-01-01T23:59:59.000Z

129

Lightning and radar observations of hurricane Rita landfall  

SciTech Connect

Los Alamos National Laboratory (LANL) owns and operates an array of Very-Low Frequency (VLF) sensors that measure the Radio-Frequency (RF) waveforms emitted by Cloud-to-Ground (CG) and InCloud (IC) lightning. This array, the Los Alamos Sferic Array (LASA), has approximately 15 sensors concentrated in the Great Plains and Florida, which detect electric field changes in a bandwidth from 200 Hz to 500 kHz (Smith et al., 2002). Recently, LANL has begun development of a new dual-band RF sensor array that includes the Very-High Frequency (VHF) band as well as the VLF. Whereas VLF lightning emissions can be used to deduce physical parameters such as lightning type and peak current, VHF emissions can be used to perform precise 3d mapping of individual radiation sources, which can number in the thousands for a typical CG flash. These new dual-band sensors will be used to monitor lightning activity in hurricanes in an effort to better predict intensification cycles. Although the new LANL dual-band array is not yet operational, we have begun initial work utilizing both VLF and VHF lightning data to monitor hurricane evolution. In this paper, we present the temporal evolution of Rita's landfall using VLF and VHF lightning data, and also WSR-88D radar. At landfall, Rita's northern eyewall experienced strong updrafts and significant lightning activity that appear to mark a transition between oceanic hurricane dynamics and continental thunderstorm dynamics. In section 2, we give a brief overview of Hurricane Rita, including its development as a hurricane and its lightning history. In the following section, we present WSR-88D data of Rita's landfall, including reflectivity images and temporal variation. In section 4, we present both VHF and VLF lightning data, overplotted on radar reflectivity images. Finally, we discuss our observations, including a comparison to previous studies and a brief conclusion.

Henderson, Bradley G [Los Alamos National Laboratory; Suszcynsky, David M [Los Alamos National Laboratory; Hamlin, Timothy E [Los Alamos National Laboratory; Jeffery, C A [Los Alamos National Laboratory; Wiens, Kyle C [TEXAS TECH U.; Orville, R E [TEXAS A& M

2009-01-01T23:59:59.000Z

130

An Integrated Docking Pipeline for the Prediction of Large-Scale Protein-Protein Interactions  

E-Print Network (OSTI)

An Integrated Docking Pipeline for the Prediction of Large-Scale Protein-Protein Interactions Xin. In this study, we developed a protein-protein docking pipeline (PPDP) that integrates a variety of state studies. In this study, we developed a protein-protein docking pipeline by integrat

131

Average Diurnal Variation of Summer Lightning over the Floirida Peninsula  

Science Conference Proceedings (OSTI)

Data derived from a large network of electric field mills have been used to determine the average diurnal variation of lightning in a Florida seacoast environment. These data were obtained at the NASA Kennedy Space Center (KSC) and the Cape ...

Launa M. Maier; E. Philip Krider; Michael W. Maier

1984-06-01T23:59:59.000Z

132

Theory and observations of controls on lightning flash size spectra  

Science Conference Proceedings (OSTI)

Previous analyses of VHF Lightning Mapping Array (LMA) observations relative to the location of deep convective updrafts have noted a systematic pattern in flash characteristics. In and near strong updrafts, flashes tend to be smaller and more ...

Eric C. Bruning; Donald R. MacGorman

133

Lightning during Two Central U.S. Winter Precipitation Events  

Science Conference Proceedings (OSTI)

Network-detected cloud-to-ground lightning coincident with mainly frozen precipitation (freezing rain, sleet, snow) was studied over the central United States during two outbreaks of arctic air in January 1994. During the first event, the ratio ...

Ronald L. Holle; Andrew I. Watson

1996-12-01T23:59:59.000Z

134

The Limiting Accuracy of Long Wavelength Lightning Flash Location  

Science Conference Proceedings (OSTI)

Many recently developed systems for lightning flash location use frequencies of hundreds of kHz or higher to achieve enhanced precision, although this reduces potential range. However, for coherent systems operating at high signal-to-noise ratios ...

Anthony C. L. Lee

1989-02-01T23:59:59.000Z

135

The Definition of GOES Infrared Lightning Initiation Interest Fields  

Science Conference Proceedings (OSTI)

Within cumulus cloud fields that develop in conditionally unstable air masses, only a fraction of the cumuli may eventually develop into deep convection. Identifying which of these convective clouds is most likely to generate lightning often ...

Ryan J. Harris; John R. Mecikalski; Wayne M. MacKenzie Jr.; Philip A. Durkee; Kurt E. Nielsen

2010-12-01T23:59:59.000Z

136

Three-Dimensional VHF Lightning Mapping System for Winter Thunderstorms  

Science Conference Proceedings (OSTI)

A three-dimensional (3D) winter lightning mapping system employing very high frequency (VHF) broadband signals was developed for continuous remote observation in winter. VHF broadband pulses radiated by leader progression are received with three ...

Masahide Nishihashi; Ken-ichi Shimose; Kenichi Kusunoki; Syugo Hayashi; Ken-ichiro Arai; Hanako Y. Inoue; Wataru Mashiko; Masako Kusume; Hiroyuki Morishima

2013-02-01T23:59:59.000Z

137

Low-Light-Level Television Measurements of Lightning  

Science Conference Proceedings (OSTI)

Nighttime observations of lightning were conducted using a low-light-level television system at the Langmuir Laboratory for Atmospheric Research in New Mexico. The number of strokes per flash, the interstroke intervals, and flash durations of ...

K. Stuart Clifton; G. Kelly Hill; George C. Marshall

1980-09-01T23:59:59.000Z

138

Cloud-to-Ground Lightning Activity in Mesoscale Convective Complexes  

Science Conference Proceedings (OSTI)

A study of cloud-to-ground lightning activity attending an important subclass of mesoscale convective weather systems called the mesoscale convective complex shows that groun discharge flash rates in excess of 1000 h?1 can be sustained on average ...

S. J. Goodman; D. R. MacGorman

1986-12-01T23:59:59.000Z

139

Lightning Direction-Finding Systems for Forest Fire Detection  

Science Conference Proceedings (OSTI)

Extensive networks of magnetic direction-finding (DF) stations have been installed throughout the western United States and Alaska to facilitate early detection of lightning-caused fires. Each station contains a new wideband direction-finder that ...

E. P. Krider; R. C. Noggle; A. E. Pifer; D. L. Vance

1980-09-01T23:59:59.000Z

140

Relationships between Convective Storm Kinematics, Precipitation, and Lightning  

Science Conference Proceedings (OSTI)

Combined multiparameter radar, dual-Doppler, thermodynamic sounding, and lightning observations of 11 thunderstorms (6 from the midlatitudes, 5 from the Tropics) are examined. The thunderstorms span a wide spectrum of intensities, from weak ...

Timothy J. Lang; Steven A. Rutledge

2002-10-01T23:59:59.000Z

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


141

A Total Lightning Trending Algorithm to Identify Severe Thunderstorms  

Science Conference Proceedings (OSTI)

An algorithm that provides an early indication of impending severe weather from observed trends in thunderstorm total lightning flash rates has been developed. The algorithm framework has been tested on 20 thunderstorms, including 1 nonsevere ...

Patrick N. Gatlin; Steven J. Goodman

2010-01-01T23:59:59.000Z

142

Insured Lightning-Caused Property Damage in Three Western States  

Science Conference Proceedings (OSTI)

Insurance claims resulting from lightning damage in Colorado, Utah, and Wyoming were analyzed during the period from 1987 to 1993. Most claims were from personal accounts, while some were commercial.

Ronald L. Holle; Ral E. Lpez; Lowell J. Arnold; John Endres

1996-08-01T23:59:59.000Z

143

Theory and Observations of Controls on Lightning Flash Size Spectra  

Science Conference Proceedings (OSTI)

Previous analyses of very high frequency (VHF) Lightning Mapping Array (LMA) observations relative to the location of deep convective updrafts have noted a systematic pattern in flash characteristics. In and near strong updrafts, flashes tend to ...

Eric C. Bruning; Donald R. MacGorman

2013-12-01T23:59:59.000Z

144

Mitigating Lightning Damage at Grower and Shipper of Fresh Vegetables  

Science Conference Proceedings (OSTI)

Lightning-related weather disturbances can cause significant power quality (PQ) issues. Inadequate and poorly maintained lightning protection systems add to the problem and often result in widespread PQ disturbances whose source may be difficult to trace. Facilities encountering such widespread PQ disturbances eventually need a detailed PQ audit to locate, fix, and harden the plant against PQ disturbances. This case study details the findings and recommendations of one such audit conducted at the process...

2007-12-31T23:59:59.000Z

145

Acceptance of Lightning Detectors and Localization Systems under Different Damping Conditions  

Science Conference Proceedings (OSTI)

The acceptance of individual lightning detectors, idealized detection networks using both loop antenna and time of arrival techniques, and the Swedish lightning localization network have been investigated. The calculations were based on Weibull-...

Th Schtte; E. Piler; D. Filipovic; S. Israelsson

1987-09-01T23:59:59.000Z

146

Observed Lightning Morphology Relative to Modeled Space Charge and Electric Field Distributions in a Tornadic Storm  

Science Conference Proceedings (OSTI)

This study uses a kinematic numerical cloud model that includes electrification and lightning discharge processes to investigate hypotheses concerning intracloud lightning flash rates in the Binger, Oklahoma, tornadic storm of 22 May 1981. ...

Conrad L. Ziegler; Donald R. MacGorman

1994-03-01T23:59:59.000Z

147

Observations of Lightning in Convective Supercells within Tropical Storms and Hurricanes  

Science Conference Proceedings (OSTI)

Cloud-to-ground (CG) lightning observations from land-based lightning detection networks now allow monitoring this component of the electrical structure of tropical storms and hurricanes within a few hundred kilometers of the United States ...

Walter A. Lyons; Cecil S. Keen

1994-08-01T23:59:59.000Z

148

A Statistical Procedure to Forecast Warm Season Lightning over Portions of the Florida Peninsula  

Science Conference Proceedings (OSTI)

Sixteen years of cloud-to-ground lightning data from the National Lightning Detection Network and morning radiosonde-derived parameters are used to develop a statistical scheme to provide improved forecast guidance for warm season afternoon and ...

Phillip E. Shafer; Henry E. Fuelberg

2006-10-01T23:59:59.000Z

149

Lake-Effect Snowstorms in Northern Utah and Western New York with and without Lightning  

Science Conference Proceedings (OSTI)

Lake-effect snowstorms in northern Utah and western New York with and without lightning/thunder are examined. Lake-effect snowstorms with lightning have significantly higher temperatures and dewpoints in the lower troposphere and significantly ...

David M. Schultz

1999-12-01T23:59:59.000Z

150

Total Lightning Characteristics Relative to Radar and Satellite Observations of Oklahoma Mesoscale Convective Systems  

Science Conference Proceedings (OSTI)

The advent of regional very high frequency (VHF) Lightning Mapping Arrays (LMAs) makes it possible to begin analyzing trends in total lightning characteristics in ensembles of mesoscale convective systems (MCSs). Flash initiations observed by the ...

Jeffrey A. Makowski; Donald R. MacGorman; Michael I. Biggerstaff; William H. Beasley

2013-05-01T23:59:59.000Z

151

Spatial and Temporal Distributions of Lightning over Arizona from a Power Utility Perspective  

Science Conference Proceedings (OSTI)

This study was designed to determine whether a spatially significant and temporally persistent variation in cloud-to-ground lightning frequency exists across the Salt River Project (SRP) region of central Arizona. Cloud-to-ground lightning data ...

Ral E. Lpez; Ronald L. Holle; Andrew I. Watson; Jon Skindlov

1997-06-01T23:59:59.000Z

152

A Framework for the Statistical Analysis of Large Radar and Lightning Datasets: Results from STEPS 2000  

Science Conference Proceedings (OSTI)

A framework for the statistical analysis of large radar and lightning datasets is described and implemented in order to analyze two research questions in atmospheric electricity: storms dominated by positive cloud-to-ground (+CG) lightning and ...

Timothy J. Lang; Steven A. Rutledge

2011-08-01T23:59:59.000Z

153

Lightning Activity in a Hail-Producing Storm Observed with Phased-Array Radar  

Science Conference Proceedings (OSTI)

This study examined lightning activity relative to the rapidly evolving kinematics of a hail-producing storm on 15 August 2006. Data were provided by the National Weather Radar Testbed Phased-Array Radar, the Oklahoma Lightning Mapping Array, and ...

C. Emersic; P. L. Heinselman; D. R. MacGorman; E. C. Bruning

2011-06-01T23:59:59.000Z

154

A Comparative Analysis of the Temporal Variability of Lightning Observations and GOES Imagery  

Science Conference Proceedings (OSTI)

Lightning Positioning and Tracking System (LPATS) data received by the Cooperative Institute for Research in the Atmosphere via a real-time weather data network were used to study the temporal variability of lightning for a frontal system and ...

P. B. Roohr; T. H. Vonder Haar

1994-11-01T23:59:59.000Z

155

Daylight Spectra of Individual Lightning Flashes in the 370690 nm Region  

Science Conference Proceedings (OSTI)

An optical multichannel analyzer slit spectrometer coupled to a minicomputer was used to record lightning spectra. This is the first successful application of a slit spectrometer to the study of individual lightning flashes and it was ...

Richard E. Orville

1980-04-01T23:59:59.000Z

156

Correlating Lightning to Severe Local Storms in the Northeastern United States  

Science Conference Proceedings (OSTI)

Two tornado events and an intense downburst episode were investigated in an attempt to relate cloud-to-ground lightning rates with the occurrence of severe local storms. The State University of New York at Albany lightning detection system was ...

Richard J. Kane

1991-03-01T23:59:59.000Z

157

Meteorological and Electrical Conditions Associated with Positive Cloud-to-Ground Lightning  

Science Conference Proceedings (OSTI)

Meteorological and electrical conditions associated with the occurrence of positive cloud-to-ground (CG) lightning (i.e., lightning that lowers positive charge to ground) are examined. Results from case studies in winter and summer storms reveal ...

Cynthia D. Engholm; Earle R. Williams; Randall M. Dole

1990-02-01T23:59:59.000Z

158

Warm Season Lightning Probability Prediction for Canada and the Northern United States  

Science Conference Proceedings (OSTI)

Statistical models valid MaySeptember were developed to predict the probability of lightning in 3-h intervals using observations from the North American Lightning Detection Network and predictors derived from Global Environmental Multiscale (GEM)...

William R. Burrows; Colin Price; Laurence J. Wilson

2005-12-01T23:59:59.000Z

159

The Impact of a 2 CO2 Climate on Lightning-Caused Fires  

Science Conference Proceedings (OSTI)

Future climate change could have significant repercussions for lightning-caused wildfires. Two empirical fire models are presented relating the frequency of lightning fires and the area burned by these fires to the elective precipitation and the ...

Colin Price; David Rind

1994-10-01T23:59:59.000Z

160

The Case for Using Blunt-Tipped Lightning Rods as Strike Receptors  

Science Conference Proceedings (OSTI)

Conventional lightning rods used in the United States have sharp tips, a practice derived from Benjamin Franklin's discovery of a means to obtain protection from lightning. However, the virtue of sharp tips for strike reception has never been ...

C. B. Moore; G. D. Aulich; William Rison

2003-07-01T23:59:59.000Z

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


161

Documenting Storm Severity in the Mid-Atlantic Region Using Lightning and Radar Information  

Science Conference Proceedings (OSTI)

Storm severity in the mid-Atlantic region of the United States is examined using lightning, radar, and model-derived information. Automated Warning Decision Support System (WDSS) procedures are developed to create grids of lightning and radar ...

Scott D. Rudlosky; Henry E. Fuelberg

2013-09-01T23:59:59.000Z

162

Effects of Charge Distribution in Thunderstorms on Lightning Propagation Paths in Darwin, Australia  

Science Conference Proceedings (OSTI)

The charge distributions in a thundercloud play an important role in the initiation and propagation of lightning discharges. To further understand the effects of charge distributions on lightning discharge, the authors conducted a very high-...

Manabu Akita; Satoru Yoshida; Yoshitaka Nakamura; Takeshi Morimoto; Tomoo Ushio; Zen Kawasaki; Daohong Wang

2011-04-01T23:59:59.000Z

163

Improving Convective Precipitation Forecasting through Assimilation of Regional Lightning Measurements in a Mesoscale Model  

Science Conference Proceedings (OSTI)

A technique developed for assimilating regional lightning measurements into a meteorological model is presented in this paper. The goal is to assess the effectiveness of cloud-to-ground (CG) lightning information for improving the convective ...

Anastasios Papadopoulos; Themis G. Chronis; Emmanouil N. Anagnostou

2005-07-01T23:59:59.000Z

164

The Morphology of Eyewall Lightning Outbreaks in Two Category 5 Hurricanes  

Science Conference Proceedings (OSTI)

Data from the Long-Range Lightning Detection Network (LLDN), the Tropical Rainfall Measuring Mission (TRMM) satellite, and reconnaissance aircraft are used to analyze the morphology of lightning outbreaks in the eyewalls of Hurricanes Rita and ...

K. Squires; S. Businger

2008-05-01T23:59:59.000Z

165

The North American Lightning Detection Network (NALDN)First Results: 19982000  

Science Conference Proceedings (OSTI)

Cloud-to-ground lightning data have been analyzed for the years 19982000 for North America (Canada plus the contiguous United States) for all ground flashes, positive flashes, the percentage of positive lightning, peak currents for negative and ...

Richard E. Orville; Gary R. Huffines; William R. Burrows; Ronald L. Holle; Kenneth L. Cummins

2002-08-01T23:59:59.000Z

166

Polarimetric and Electrical Characteristics of a Lightning Ring in a Supercell Storm  

Science Conference Proceedings (OSTI)

On 30 May 2004, a supercell storm was sampled by a suite of instrumentation that had been deployed as part of the Thunderstorm Electrification and Lightning Experiment (TELEX). The instrumentation included the Oklahoma Lightning Mapping Array (OK-...

Clark D. Payne; Terry J. Schuur; Donald R. MacGorman; Michael I. Biggerstaff; Kristin M. Kuhlman; W. David Rust

2010-06-01T23:59:59.000Z

167

Relationships between Lightning Location and Polarimetric Radar Signatures in a Small Mesoscale Convective System  

Science Conference Proceedings (OSTI)

On 19 June 2004, the Thunderstorm Electrification and Lightning Experiment observed electrical, microphysical, and kinematic properties of a small mesoscale convective system (MCS). The primary observing systems were the Oklahoma Lightning ...

Nicole R. Lund; Donald R. MacGorman; Terry J. Schuur; Michael I. Biggerstaff; W. David Rust

2009-12-01T23:59:59.000Z

168

Lightning Casualties and Damages in the United States from 1959 to 1994  

Science Conference Proceedings (OSTI)

Lightning-caused fatalities, injuries, and damage reports for the United States are listed in the National Oceanic and Atmospheric Administration publication Storm Data. Previously published studies of lightning casualties and damages in the ...

E. Brian Curran; Ronald L. Holle; Ral E. Lpez

2000-10-01T23:59:59.000Z

169

Exploring Cloud-to-Ground Lightning Earth Highpoint Attachment Geography by Peak Current  

Science Conference Proceedings (OSTI)

This study applied remotely sensed cloud-to-ground (CG) lightning strike location data, a digital elevation model (DEM), and a geographic information system (GIS) to characterize negative polarity peak current CG lightning Earth attachment ...

Brandon J. Vogt

2011-02-01T23:59:59.000Z

170

The Daily Cloud-to-Ground Lightning Flash Density in the Contiguous United States and Finland  

Science Conference Proceedings (OSTI)

A method is developed to quantify thunderstorm intensity according to cloud-to-ground lightning flashes (hereafter ground flashes) determined by a lightning-location sensor network. The method is based on the ground flash density ND per ...

Antti Mkel; Pekka Rossi; David M. Schultz

2011-05-01T23:59:59.000Z

171

Laboratory Calibration of the Optical Transient Detector and the Lightning Imaging Sensor  

Science Conference Proceedings (OSTI)

The authors present in detail the laboratory apparatus and techniques that were used to complete a full radiometric calibration of two space-based lightning detectors: the optical transient detector (OTD) and the lightning imaging sensor (LIS) ...

William J. Koshak; Mike F. Stewart; Hugh J. Christian; James W. Bergstrom; John M. Hall; Richard J. Solakiewicz

2000-07-01T23:59:59.000Z

172

North Alabama Lightning Mapping Array (LMA): VHF Source Retrieval Algorithm and Error Analyses  

Science Conference Proceedings (OSTI)

Two approaches are used to characterize how accurately the north Alabama Lightning Mapping Array (LMA) is able to locate lightning VHF sources in space and time. The first method uses a Monte Carlo computer simulation to estimate source retrieval ...

W. J. Koshak; R. J. Solakiewicz; R. J. Blakeslee; S. J. Goodman; H. J. Christian; J. M. Hall; J. C. Bailey; E. P. Krider; M. G. Bateman; D. J. Boccippio; D. M. Mach; E. W. McCaul; M. F. Stewart; D. E. Buechler; W. A. Petersen; D. J. Cecil

2004-04-01T23:59:59.000Z

173

Highlights of a New Ground-Based, Hourly Global Lightning Climatology  

Science Conference Proceedings (OSTI)

The seasonally and diurnally varying frequency of lightning flashes provides a measure of the frequency of occurrence of intense convection and, as such, is useful in describing the Earth's climate. Here we present a few highlights of a global lightning ...

Katrina S. Virts; John M. Wallace; Michael L. Hutchins; Robert H. Holzworth

2013-09-01T23:59:59.000Z

174

Winter Lightning and Heavy Frozen Precipitation in the Southeast United States  

Science Conference Proceedings (OSTI)

This study addresses winter season lightning by examining synoptic-scale circulations, cloud-to-ground (CG) lightning patterns, and frozen precipitation. Specifically, locations, frequencies, and polarities of CG flashes are related to the ...

S. M. Hunter; S. J. Underwood; R. L. Holle; T. L. Mote

2001-08-01T23:59:59.000Z

175

Radar and Lightning Observations of Normal and Inverted Polarity Multicellular Storms from STEPS  

Science Conference Proceedings (OSTI)

This study discusses radar and lightning observations of two multicellular storms observed during the Severe Thunderstorm Electrification and Precipitation Study. The Lightning Mapping Array data indicated that the charge structure of the 19 June ...

Sarah A. Tessendorf; Steven A. Rutledge; Kyle C. Wiens

2007-11-01T23:59:59.000Z

176

Return-Stroke Multiplicity of Negative Cloud-to-Ground Lightning Flashes  

Science Conference Proceedings (OSTI)

The percentage of negative lightning flashes composed of a single stroke is examined. This percentage is estimated from data reported by the U.S. National Lightning Detection Network (NLDN) for Florida and New Mexico and is compared with that ...

Vladimir A. Rakov; Gary R. Huffines

2003-10-01T23:59:59.000Z

177

Comparing a Regional, Subcontinental, and Long-Range Lightning Location System over the Benelux and France  

Science Conference Proceedings (OSTI)

Increasing possibilities for using lightning datafor instance, in monitoring and tracking applicationsnecessitate proper spatial and temporal mapping of lightning events. It is therefore of importance to assess the capabilities and limitations ...

Dieter R. Poelman; Franoise Honor; Graeme Anderson; Stphane Pedeboy

2013-10-01T23:59:59.000Z

178

Summertime Cloud-to-Ground Lightning Activity around Major Midwestern Urban Areas  

Science Conference Proceedings (OSTI)

Cloud-to-ground lightning flash data collected by the National Lightning Detection Network were analysed in and around 16 central U.S. cities for the period 1989?92. Lightning data are well suited to study storm activity in and around large urban ...

Nancy E. Westcott

1995-07-01T23:59:59.000Z

179

Dynamic Simulation of Lightning Attachment to Earthed Overhead Transmission Line Structures  

Science Conference Proceedings (OSTI)

In this paper a novel method is presented to investigate lightning stroke attachment to earthed overhead transmission line structures. This method is based on the dynamic simulation of lightning downward leader movement toward the ground in three dimensions. ... Keywords: charge simulation method, downward leader, lightning, transmission line, upward leader

Mohammad Reza Bank Tavakoli; Behrooz Vahidi

2010-07-01T23:59:59.000Z

180

LPAT: An interactive simulation tool for assessing the lightning performance of Hellenic high voltage transmission lines  

Science Conference Proceedings (OSTI)

The paper presents the Lightning Performance Assessment Tool (LPAT), an interactive simulation tool which assesses the lightning performance of high voltage transmission lines. The LPAT design was based on a recently published methodology that has been ... Keywords: Lightning performance, Optical transient density, Overhead transmission lines, Simulation, Software tool, User interface

P. Karampelas; L. Ekonomou; S. Panetsos; G. E. Chatzarakis

2011-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


181

Global lightning and severe storm monitoring from GPS orbit  

Science Conference Proceedings (OSTI)

Over the last few decades, there has been a growing interest to develop and deploy an automated and continuously operating satellite-based global lightning mapper [e.g. Christian et al., 1989; Weber et al., 1998; Suszcynsky et al., 2000]. Lightning is a direct consequence of the electrification and breakdown processes that take place during the convective stages of thunderstorm development. Satellite-based lightning mappers are designed to exploit this relationship by using lightning detection as a proxy for remotely identifying, locating and characterizing strong convective activity on a global basis. Global lightning and convection mapping promises to provide users with (1) an enhanced global severe weather monitoring and early warning capability [e.g. Weber et al., 1998] (2) improved ability to optimize aviation flight paths around convective cells, particularly over oceanic and remote regions that are not sufficiently serviced by existing weather radar [e.g. Weber et al., 1998], and (3) access to regional and global proxy data sets that can be used for scientific studies and as input into meteorological forecast and global climatology models. The physical foundation for satellite-based remote sensing of convection by way of lightning detection is provided by the basic interplay between the electrical and convective states of a thundercloud. It is widely believed that convection is a driving mechanism behind the hydrometeor charging and transport that produces charge separation and lightning discharges within thunderclouds [e.g. see chapter 3 in MacGorman and Rust, 1998]. Although cloud electrification and discharge processes are a complex function of the convective dynamics and microphysics of the cloud, the fundamental relationship between convection and electrification is easy to observe. For example, studies have shown that the strength of the convective process within a thundercell can be loosely parameterized (with large variance) by the intensity of the electrical activity within that cell as measured by the lightning flash rate. Williams [2001] has provided a review of experimental work that shows correlations between the total lightning flash rate and the fifth power of the radar cloud-top height (i.e. convective strength) of individual thunder cells. More recently, Ushio et al., [2001] used a large statistical sampling of optical data from the Lightning Imaging Sensor (LIS) in conjunction with data provided by the Precipitation Radar (PR) aboard the Tropical Rainfall Monitoring Mission (TRMM) satellite to conclude that the total lightning flash rate increases exponentially with storm height. Lightning activity levels have also been correlated to cloud ice content, a basic product of the convective process. For example, Blyth et al. [2001] used the Thermal Microwave Imager (TMI) aboard the TRMM satellite to observe a decrease in the 37 and 85 GHz brightness temperatures of upwelling terrestrial radiation during increased lightning activity. This reduction in brightness temperature is believed to be the result of increased ice scattering in the mixed phase region of the cloud. Toracinta and Zipser [2001] have found similar relationships using the Optical Transient Detector (OTD) satellite instrument and the Special Sensor Microwave Imager (SSM/I) aboard the DMSP satellites.

Suszcynsky, D. M. (David M.); Jacobson, A. R.; Linford, J (Justin); Pongratz, M. B. (Morris B.); Light, T. (Tracy E.); Shao, X. (Xuan-Min)

2004-01-01T23:59:59.000Z

182

Oliktok Dock allows quick offloading of oil field modules  

SciTech Connect

A discussion of the design and construction of the Oliktok Dock, which serves the Kuparuk River oil fields of the Alaskan North Slope, was presented. The dockhead construction required 18,000 linear ft of HZ 800-16 Arbed sheetpile walls which extended from an elevation of -40 to +10 ft. The project began in mid-summer and was completed in November and cost $5 million.

Not Available

1983-04-01T23:59:59.000Z

183

National Renewable Energy Laboratory program on lightning risk and wind turbine generator protection  

DOE Green Energy (OSTI)

This paper will describe the NREL program for addressing lightning protection for wind turbines. A test program will begin this summer at the Central and South West Services Inc. (CSW) wind farm near Fort Davis, Texas, to assess lightning risk, the frequency of lightning strikes on wind turbines compared to risk assessment predictions, and the effectiveness of some protection techniques. A Web page will be assembled to provide resources for designers and operators and feedback for issues as they arise. Also, a database of lightning events (and corresponding damage) will be collected to assist in maturing the understanding of wind turbine lightning protection.

Muljadi, E. [National Renewable Energy Lab., Golden, CO (United States); McNiff, B. [McNiff Light Industry, Blue Hill, ME (United States)

1997-09-01T23:59:59.000Z

184

Total lightning observations of severe convection over North Texas  

E-Print Network (OSTI)

Five severe convective cells over North Texas from three separate dates were examined to determine what three dimensional, or total lightning data can add to the understanding of a convective cells intensity, propagation, and severe weather potential. Total lightning data were obtained from Vaisala Inc.s Dallas/Fort Worth (D/FW) Lightning Detection and Ranging (LDAR) network. Radar data from two Weather Surveillance Radar 1988 Doppler (WSR-88D) sites were used for position data and information regarding the intensity and kinematic properties of each cell. Total lightning products used by the National Weather Service Forecast Office in Fort Worth, Texas were compared to total lightning flash rate; a quantity that has been shown to be correlated to changes in cell intensity inferred from other sources, such as radar and satellite data. These products, specifically flash extent density (FED) were also compared to CG flash rate and radar derived measures from the WSR-88D sites. The results of this work show that FED and total flash rate are well correlated, with an average Pearson correlation value of 0.73, indicating that previous total flash rate results may also apply to FED. Lightning hooks, holes, and notches in FED displays indicated likely updraft regions, while appendages were observed to develop prior to deviant motion with two supercells. These results, combined with a greater update frequency provided a useful complement to radar data in the warning decision process. FED jumps were observed prior to several severe weather reports, indicating that total lightning activity may be related to updraft strength as found in past studies. However, FED jumps were sometimes observed without any associated severe event. More work is clearly needed to define what FED changes are of most importance in the short-term prediction of storm severity. The usefulness of the total lightning data on these dates was dependant upon LDAR network status and distance of the cell from the network center. The results of this study suggest that combining total flash rate trends with visual displays of FED provides the greatest added benefit to forecasters in maintaining situational awareness during warning operations.

McKinney, Christopher Michael

2008-08-01T23:59:59.000Z

185

Carbon dioxide emission during forest fires ignited by lightning  

E-Print Network (OSTI)

In this paper we developed the model for the carbon dioxide emission from forest fire. The master equation for the spreading of the carbon dioxide to atmosphere is the hyperbolic diffusion equation. In the paper we study forest fire ignited by lightning. In that case the fores fire has the well defined front which propagates with finite velocity.

Magdalena Pelc; Radoslaw Osuch

2009-03-31T23:59:59.000Z

186

Ground Truth: A Positive Cloud-to-Ground Lightning Flash  

Science Conference Proceedings (OSTI)

We describe the circumstances and effects of a cloud-to-ground lightning flash that lowered positive charge and struck a residential home near Albany, New York. The characteristics of the flash were detected and recorded by a wide-band magnetic ...

Vincent P. Idone; Richard E. Orville; Ronald W. Henderson

1984-07-01T23:59:59.000Z

187

The Effects of Clouds on the Light Produced by Lightning  

Science Conference Proceedings (OSTI)

A Monte Carlo method has been used to simulate the transport of visible (0.45 ?m) and near-infrared (0.87 ?m) photons which are produced by transient light sources, such as lightning, within cubic, spherical and cylindrical clouds. Computations ...

L. W. Thomson; E. P. Krider

1982-09-01T23:59:59.000Z

188

Switching Surge and Lightning Impact on Increasing Power Flow  

Science Conference Proceedings (OSTI)

This report describes the effect of transient overvoltages on increasing the power flow on the electric power transmission system. The report focuses on the effect of system changes on transient recovery voltage (TRV) duties imposed on circuit breakers, and the impact of switching surges, lightning, and sustained overvoltages on transmission line upgrading.

2006-12-13T23:59:59.000Z

189

A Small Updraft Producing a Fatal Lightning Flash  

Science Conference Proceedings (OSTI)

Just prior to 1900 UTC 25 July 2000, an 18-year-old male was fatally wounded by a lightning flash on the summit of Pikes Peak, Colorado. This case is believed to be unique in that radar and satellite data indicated that the cell that produced the ...

Stephen Hodanish; Ronald L. Holle; Daniel T. Lindsey

2004-06-01T23:59:59.000Z

190

Forecasting Lightning Threat Using Cloud-Resolving Model Simulations  

Science Conference Proceedings (OSTI)

Two new approaches are proposed and developed for making time- and space-dependent, quantitative short-term forecasts of lightning threats, and a blend of these approaches is devised that capitalizes on the strengths of each. The new methods are ...

Eugene W. McCaul Jr.; Steven J. Goodman; Katherine M. LaCasse; Daniel J. Cecil

2009-06-01T23:59:59.000Z

191

Lightning, fire and longleaf pine: Using natural disturbance to guide management.  

Science Conference Proceedings (OSTI)

Abstract The importance of lightning as an ignition source for the fire adapted longleaf pine (Pinus palustris) ecosystem is widely recognized. Lightning also impacts this system on a smaller scale by causing individual tree mortality. The objective of this study was to determine mortality due to lightning and other agents in longleaf stands on the Ocala National Forest in central Florida and to quantify lightning ignited fire. Mortality from lightning was also tracked in longleaf stands on the Savannah River Site in South Carolina. Lightning killed more trees than any other agent with a mean mortality of nearly 1 tree/3 ha/yr in Florida and 1 tree/8 ha/yr in South Carolina. The probability of a tree being struck by lightning increased as a function of tree height at both sites, i.e. lightning preferentially removed the largest trees from the stand. In addition lightning strikes were clumped within stands, sometimes killed multiple trees with a single strike, and often hit trees on the edge of existing gaps. The combination of these processes means gaps suitable for regeneration within longleaf stands are created quite rapidly. This information provides guidelines for the development of selection harvest systems based on this natural disturbance. Although lightning activity was greatest during the summer months in Florida and most fires occurred in June, the probability of a strike causing a fire was highest in February to May. Published by Elsevier B.V.

Kenneth W. Outcalt

2008-02-01T23:59:59.000Z

192

Cloud-to-ground lightning characteristics over Houston, Texas  

E-Print Network (OSTI)

The U. S. National Lightning Detection Network (NLDN) has recorded cloud-to-ground (CG) lightning observations regularly for more than a decade (1989-2000). The main research focus using the recorded NLDN data has been the creation of lightning climatologies (e.g. Orville and Huffines 1999). These climatologies have revealed important results that indicated certain patterns of lightning behavior across the U. S. One of the most significant findings from such work has been the observance of lightning "hot spots" over and near cities (Westcott 1995). Houston, TX is one such "hot spot," with significant enhancement (45%, all 12 years) of lightning activity observed over and downwind of the urban area. Enhancement varied based on season and time of day, with the greatest enhancements occurring during the summer (58%) and the late morning/afternoon time periods in each season. Two other interesting features discovered during this study included a decrease (-12%) in the percentage of flashes lowering positive charge to ground over the city, and significantly larger values of negative median peak current measured along the coast and well into the Gulf of Mexico. One hypothesis proposed for explaining the Houston enhancement includes the complex sea breeze and associated low-level convergence that result because of the proximity of Galveston Bay to the southeast of Houston. Also, there are a multitude of factors associated with a large city such as Houston that can modify the local climate. Some of these urban factors include: increased cloud condensation nuclei (CCN) concentrations, especially from industrial pollution, the urban heat island, and frictional lift. It has been proposed by Rosenfeld and Lensky (1998) that the large quantity of CCN in polluted areas can enhance a storm's electrical state because they act to decrease the coalescence and rainout cloud processes, therefore allowing more supercooled liquid water to exist within the storm. The urban heat island can enhance thunderstorm initiation through increasing the low-level mesoscale convergence and upward motion directly over the city. The observations showed support for each one of these aspects to be a factor in enhanced lightning activity over Houston.

Steiger, Scott Michael

2001-01-01T23:59:59.000Z

193

Waste Isolation Pilot Plant TruDock crane system analysis  

SciTech Connect

The WIPP TruDock crane system located in the Waste Handling Building was identified in the WIPP Safety Analysis Report (SAR), November 1995, as a potential accident concern due to failures which could result in a dropped load. The objective of this analysis is to evaluate the frequency of failure of the TruDock crane system resulting in a dropped load and subsequent loss of primary containment, i.e. drum failure. The frequency of dropped loads was estimated to be 9.81E-03/year or approximately one every 102 years (or, for the 25% contingency, 7.36E-03/year or approximately one every 136 years). The dominant accident contributor was the failure of the cable/hook assemblies, based on failure data obtained from NUREG-0612, as analyzed by PLG, Inc. The WIPP crane system undergoes a rigorous test and maintenance program, crane operation is discontinued following any abnormality, and the crane operator and load spotter are required to be trained in safe crane operation, therefore it is felt that the WIPP crane performance will exceed the data presented in NUREG-0612 and the estimated failure frequency is felt to be conservative.

Morris, B.C. [Westinghouse Electric Corp., Pittsburgh, PA (United States); Carter, M. [Westinghouse Electric Corp., Carlsbad, NM (United States). Waste Isolation Div.

1996-10-01T23:59:59.000Z

194

Ab Initio potential grid based docking: From High Performance Computing to In Silico Screening  

Science Conference Proceedings (OSTI)

We present a new and completely parallel method for protein ligand docking. The potential of the docking target structure is obtained directly from the electron density derived through an ab initio computation. A large subregion of the crystal structure of Isocitrate Lyase

Marc R. de Jonge; H. Maarten Vinkers; Joop H. van Lenthe; Frits Daeyaert; Ian J. Bush; Huub J. J. van Dam; Paul Sherwood; Martyn F. Guest

2007-01-01T23:59:59.000Z

195

Verification & validation by docking: a case study of agent-based models of Anopheles gambiae  

Science Conference Proceedings (OSTI)

In this paper, using the technique of docking, we perform verification & validation (V&V) of agent-based simulation models that simulate the life cycle of Anopheles gambiae, the primary vector for malaria transmission. Working with one ... Keywords: Anopheles gambiae, docking, malaria epidemiology, verification & validation

S. M. Niaz Arifin; Gregory J. Davis; Ying Zhou

2010-07-01T23:59:59.000Z

196

Lightning Frequency and Microphysical Properties of Precipitating Clouds over the Western North Pacific during Winter as Derived from TRMM Multisensor Observations  

Science Conference Proceedings (OSTI)

Tropical Rainfall Measuring Mission observations from multiple sensors including precipitation radar, microwave and infrared radiometers, and a lightning sensor were used to describe precipitation, lightning frequency, and microphysical ...

Yasu-Masa Kodama; Haruna Okabe; Yukie Tomisaka; Katsuya Kotono; Yoshimi Kondo; Hideyuki Kasuya

2007-06-01T23:59:59.000Z

197

Cloud-to-Ground Lightning in the United States: NLDN Results in the First Decade, 198998  

Science Conference Proceedings (OSTI)

The physical and geographical characteristics of over 216 million cloud-to-ground lightning flashes recorded during the first decade (198998) of operation of the National Lightning Detection Network (NLDN) covering the entire continental United ...

Richard E. Orville; Gary R. Huffines

2001-05-01T23:59:59.000Z

198

Preliminary Development and Evaluation of Lightning Jump Algorithms for the Real-Time Detection of Severe Weather  

Science Conference Proceedings (OSTI)

Previous studies have demonstrated that rapid increases in total lightning activity (intracloud + cloud-to-ground) are often observed tens of minutes in advance of the occurrence of severe weather at the ground. These rapid increases in lightning ...

Christopher J. Schultz; Walter A. Petersen; Lawrence D. Carey

2009-12-01T23:59:59.000Z

199

Study of the Periodicities of Lightning Activity in Three Main Thunderstorm Centers Based on Schumann Resonance Measurements  

Science Conference Proceedings (OSTI)

Time variations of lightning activity in the three main tropical thunderstorm centers located in the Maritime Continent (Pakistan, India, Southeast Asia, Indonesia, and Australia), Africa, and the Americas are analyzed using a lightning activity ...

Zenon Nieckarz; Stanis?aw Zi?ba; Andrzej Ku?ak; Adam Michalec

2009-12-01T23:59:59.000Z

200

Diurnal Lightning Variability over the Maritime Continent: Impact of Low-Level Winds, Cloudiness, and the MJO  

Science Conference Proceedings (OSTI)

Lightning over the Maritime Continent exhibits a pronounced diurnal cycle. Daytime and evening lightning occurs near coastlines and over mountain slopes, driven by sea and valley breezes. Nocturnal and morning thunderstorms are touched off where ...

Katrina S. Virts; John M. Wallace; Michael L. Hutchins; Robert H. Holzworth

2013-10-01T23:59:59.000Z

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


201

Pre- and Postupgrade Distributions of NLDN Reported Cloud-to-Ground Lightning Characteristics in the Contiguous United States  

Science Conference Proceedings (OSTI)

The National Lightning Detection Network (NLDN) underwent a major upgrade during 200203 that increased its sensitivity and improved its performance. It is important to examine cloud-to-ground (CG) lightning distributions before and after this ...

Scott D. Rudlosky; Henry E. Fuelberg

2010-09-01T23:59:59.000Z

202

Anomalously Low Negative Cloud-to-Ground Lightning Flash Rates in Intense Convective Storms Observed during STERAO-A  

Science Conference Proceedings (OSTI)

Concurrent measurements from the CSU-CHILL multiparameter Doppler radar, the Office National dEtudes et de Recherches Arospatiales VHF lightning interferometer, and the National Lightning Detection Network, obtained during phase A of the ...

Timothy J. Lang; Steven A. Rutledge; James E. Dye; Martin Venticinque; Pierre Laroche; Eric Defer

2000-01-01T23:59:59.000Z

203

Characteristics of Sprite-Producing Positive Cloud-to-Ground Lightning during the 19 July 2000 STEPS Mesoscale Convective Systems  

Science Conference Proceedings (OSTI)

During the summer of 2000, the Severe Thunderstorm Electrification and Precipitation Study (STEPS) program deployed a three-dimensional Lightning Mapping Array (LMA) near Goodland, Kansas. Video confirmation of sprites triggered by lightning ...

Walter A. Lyons; Thomas E. Nelson; Earle R. Williams; Steven A. Cummer; Mark A. Stanley

2003-10-01T23:59:59.000Z

204

Performance Assessment of the World Wide Lightning Location Network (WWLLN), Using the Los Alamos Sferic Array (LASA) as Ground Truth  

Science Conference Proceedings (OSTI)

The World Wide Lighting Location Network (WWLLN) locates lightning globally, using sparsely distributed very low frequency (VLF) detection stations. Due to WWLLNs detection at VLF (in this case 10 kHz), the lightning signals from strong strokes ...

Abram R. Jacobson; Robert Holzworth; Jeremiah Harlin; Richard Dowden; Erin Lay

2006-08-01T23:59:59.000Z

205

Intercomparison Study of Cloud-to-Ground Lightning Flashes Observed by KARITLDS and KLDN at South Korea  

Science Conference Proceedings (OSTI)

Concern regarding lightning activity as a precursor of severe weather is increasing. Atmospheric electricity, including lightning phenomena, is one of most serious threats to successful space launch operations. The objective of this study was to ...

Bong-Jae Kuk; Hong-Il Kim; Jong-Sung Ha; Hyo-Keun Lee

2011-01-01T23:59:59.000Z

206

A Fuzzy Logic Method for Lightning Prediction Using Thermodynamic and Kinematic Parameters from Radio Sounding Observations in South Korea  

Science Conference Proceedings (OSTI)

Lightning is one of the most troubling weather phenomena for weather forecasters at space centers. In this study, proximity sounding and lightning data were used to evaluate the utility of thermodynamic and kinematic parameters for forecasting ...

Bongjae Kuk; Hongil Kim; Jongsung Ha; Hyokeun Lee; Gyuwon Lee

2012-02-01T23:59:59.000Z

207

Evolution of Lightning Activity and Storm Charge Relative to Dual-Doppler Analysis of a High-Precipitation Supercell Storm  

Science Conference Proceedings (OSTI)

A high-precipitation tornadic supercell storm was observed on 2930 May 2004 during the Thunderstorm Electrification and Lightning Experiment. Observational systems included the Oklahoma Lightning Mapping Array, mobile balloon-borne soundings, and ...

Kristin M. Calhoun; Donald R. MacGorman; Conrad L. Ziegler; Michael I. Biggerstaff

2013-07-01T23:59:59.000Z

208

High-Resolution Simulation of the Electrification and Lightning of Hurricane Rita during the Period of Rapid Intensification  

Science Conference Proceedings (OSTI)

In this paper, a high-resolution simulation establishing relationships between lightning and eyewall convection during the rapid intensification phase of Rita will be highlighted. The simulation is an attempt to relate simulated lightning ...

Alexandre O. Fierro; Jon M. Reisner

2011-03-01T23:59:59.000Z

209

Understanding the Physics and PQ Impacts of Lightning  

Science Conference Proceedings (OSTI)

The EPRI Power Quality Knowledge program provides a wealth of resources in well-designed, readable, and accessible formats. Paramount among these resources are documents covering a wide range of PQ topics, written not only for use by busy PQ professionals, but also to be shared with important end-use customers and internal utility managers. This PQ TechWatch explores lightning, its history as filtered through human experience, and current methods and technologies employed to protect transmission and dist...

2009-08-10T23:59:59.000Z

210

Thunderstorm characteristics of cloud-to-ground lightning at the Kennedy Space Center, Florida: a study of lightning initiation signatures as indicated by Doppler radar  

E-Print Network (OSTI)

A sum-nary of lightning characteristics was developed for the NASA Kennedy Space Center (KSC). From-n a 1989-1996 data set, the spatial patterns, temporal patterns, and first stroke mean peak current were analyzed. Forty five thunderstorms were chosen due to their isolated development over KSC. Forty of the storms representing summer (May through September) airmass thunderstorms and five storms representing winter were examined for their storms-n initiation characteristics. Radar reflectivity echoes at the -10 C,-15 C, and-20 C temperature heights were associated with cloud-to-ground (CG) lightning strike locations from the National Lightning Detection Network. A distinct region of high ground flash densities can be seen over land matching the topography of the KSC coastline. A maximum of values was observed during the summer months for both negative and positive flashes. The absolute i-magnitude of negative peak currents was found to be higher than positive peak currents in Florida. Although thunderstorms can Occur at any time during the day, the diurnal distribution of lightning flashes showed that the afternoon (2000-2200 UTC) was the time of maximum lightning activity. From a time history of radar-echoes, it was found that the 30 dBZ echo detected at the-I 5'C temperature height is the best indicator of the beginning of CG lightning activity. The observed median lag time between this lightning initiation signature and the beginning of CG lightning flashes was 15.5 minutes. Other lightning initiation signatures were also examined at all three temperature heights and did not yield as successful results.

Gremillion, Michael Shane

1998-01-01T23:59:59.000Z

211

Cloud-to-ground lightning-precipitation relationships in the South Central United States  

E-Print Network (OSTI)

Despite the general coexistence of cloud-to-ground (CG) lightning and precipitation, there have been few studies on the nature of the relationship between these two phenomena. This study endeavors to explore this relationship, concentrating on three facets: the strength and variability of the linear correlation between the two parameters, and the possible benefits of including additional parameters to this relationship; the differences in several atmospheric and lightning parameters corresponding to differences in the precipitation-to-CG lightning ratio, and the search for an optimal radius for inclusion of CG lightning flashes when comparing them to point precipitation values. Six regions (each on the order of I' latitude by I' longitude) from the states of Nebraska, Kansas, Oklahoma, Texas, and Louisiana are examined. The parameters are examined on daily and/or monthly time scales. This study uses CG lightning data which were recorded by the National Lightning Detection Network (NLDN) during the months of April through October in the years 1989 to 1993. Precipitation data are included from all available first-order, second-order, and cooperative stations. Atmospheric parameters are computed from soundings in the region. A primary tool used in the analyses is the linear regression model for examining linear relations among CG lightning, precipitation, and other parameters. In addition, the t-test and Wilcoxon Rank Sum test are used to discern statistically significant differences in atmospheric and lightning parameters between groups of days categorized by the precipitation-to-CG lightning ratio. The results show that linear correlations between CG lightning and precipitation for a region are highly varied; there are generally higher correlations in regions further away from the Gulf of Mexico. The only parameter which is a significantly beneficial addition to the model which included CG lightning and precipitation is a measure of the amount of positive CG flashes. When days are categorized by a precipitation-to-CG lightning ratio, the percent positive shows the most dramatic differences, with values on "high precipitation-to-lightning" days from roughly five to twelve times the value on "low precipitation-to-lightning" days. Several other parameters show statistically significant differences as well. Lastly, tests on several different radii suggest that there is no consistent optimal radius for CG lightning-precipitation comparisons.

Sheridan, Scott Christopher

1995-01-01T23:59:59.000Z

212

Lightning Rates Relative to Tornadic Storm Evolution on 22 May 1981  

Science Conference Proceedings (OSTI)

On 22 May 1981, we acquired lightning and Doppler radar data on two tornadic storms in Oklahoma. Cloud-to-ground lightning flash rates were measured with a magnetic direction-finder network, and total flash rates in the vicinity of the ...

Donald R. MacGorman; Donald W. Burgess; Vladislav Mazur; W. David Rust; William L. Taylor; Brenda C. Johnson

1989-01-01T23:59:59.000Z

213

Lightning-Initiation Locations as a Remote Sensing Tool of Large Thunderstorm Electric Field Vectors  

Science Conference Proceedings (OSTI)

The lightning data that are recorded with a three-dimensional lightning mapping array (LMA) are compared with data from an electric field change sensor (in this case a flat-plate antenna operated both as a slow and a fast antenna). The goal ...

Christopher Maggio; Lee Coleman; Thomas Marshall; Maribeth Stolzenburg; Mark Stanley; Timothy Hamlin; Paul Krehbiel; William Rison; Ron Thomas

2005-07-01T23:59:59.000Z

214

Diurnal variations of NLDN-reported cloud-to-ground lightning in the United States  

Science Conference Proceedings (OSTI)

National maps of cloud-to-ground lightning flash density in flashes km-2 yr-1 for one or more years have been produced since the National Lightning Detection Network (NLDN) was first deployed across the contiguous 48 U.S. states in 1989. However, ...

Ronald L. Holle

215

Highlights of a new ground-based, hourly global lightning climatology  

Science Conference Proceedings (OSTI)

The seasonally and diurnally-varying frequency of lightning flashes provides a measure of the frequency of occurrence of intense convection and, as such, is useful in describing the Earth's climate. Here we present a few highlights of a global lightning ...

Katrina S. Virts; John M. Wallace; Michael L. Hutchins; Robert H. Holzworth

216

An Isolated Winter Cloud-to-Ground Lightning Flash Causing Damage and Injury in Connecticut  

Science Conference Proceedings (OSTI)

An isolated lightning flash at 1436:52 UTC 11 February 1996 struck and destroyed a house in Burlington, Connecticut, injuring an occupant of the house. A flash detected simultaneously by the National Lightning Detection Network was within 1.1 km ...

Ronald L. Holle; Ral E. Lpez; Kenneth W. Howard; Kenneth L. Cummins; Mark D. Malone; E. Philip Krider

1997-03-01T23:59:59.000Z

217

DEPARTMENT OF ATMOSPHERIC AND OCEANIC SCIENCE The Effect ENSO Has on Lightning  

E-Print Network (OSTI)

or no effect on flash rates. Historical records of lightning flash rates and lightning climatology are fairly generate greater flash rates along the Gulf Coast, while neutral or La Niña phases tend to have little by an atmospheric discharge of electricity and is most commonly found in thunderstorms (Columbia Encyclopedia, 2008

Maryland at College Park, University of

218

The Relationship of Lightning to Surface Convergence at Kennedy Space Center: A Preliminary Study  

Science Conference Proceedings (OSTI)

Cloud-to-ground lightning is a significant meteorological problem at the Kennedy Space Center (KSC). Of particular importance is the growth of lightning-bearing clouds in the vicinity of KSC, for which warnings must be considered on a daily ...

Andrew I. Watson; Ral E. Lpez; Ronald L. Holle; John R. Daugherty

1987-06-01T23:59:59.000Z

219

Development of a Long-Range Lightning Detection Network for the Pacific: Construction, Calibration, and Performance  

Science Conference Proceedings (OSTI)

The waveguide between the earths surface and the ionosphere allows very low-frequency (VLF) emissions generated by lightning, called sferics, to propagate over long distances. The new Pacific Lightning Detection Network (PacNet), as a part of a ...

Antti T. Pessi; Steven Businger; K. L. Cummins; N. W. S. Demetriades; M. Murphy; B. Pifer

2009-02-01T23:59:59.000Z

220

Relationship between Lightning Activity over Peninsular India and Sea Surface Temperature  

Science Conference Proceedings (OSTI)

This paper presents a study of spatiotemporal variation of lightning activity over Peninsular India (822N, 7288E) by using monthly satellite-based lightning flash grid (1 1) data for a period of 10 yr (19982007). The data are examined ...

M. I. R. Tinmaker; Kaushar Ali; G. Beig

2010-04-01T23:59:59.000Z

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


221

The North American Lightning Detection Network (NALDN)Analysis of Flash Data: 200109  

Science Conference Proceedings (OSTI)

Cloud-to-ground (CG) lightning data have been analyzed for the years 200109 for North America, which includes Alaska, Canada, and the lower 48 U.S. states. Flashes recorded within the North American Lightning Detection Network (NALDN) are ...

Richard E. Orville; Gary R. Huffines; William R. Burrows; Kenneth L. Cummins

2011-05-01T23:59:59.000Z

222

Lightning location characteristics and vertical structure analysis of isolated storm cells in the TOGA COARE region  

E-Print Network (OSTI)

Previous studies have investigated the difference in structure between continental and oceanic storms, and tropical versus mid-latitude storms. This is the first study that has investigated the differences in vertical structure between lightning producing storm cells, and non-lightning producing cells over the tropical oceans. The characteristics of lightning locations with respect to radar reflectivity were also examined. Lightning characteristics of flashes associated with TOGA COARE storm cells were examined. One hundred fifty-three flashes were detected by three direction finders within the study area, a 150 kilometer circle around the Vickers research vessel. Radar reflectivities and reflectivity gradients were compared to flash counts. It was found that lightning flashes tended to occur in the convective region (the region enclosed by the 30 dBZ contour) with a peak at 37 dBZ, but avoiding the highest reflectivity values. Flashes often occurred in reflectivity gradients higher than 3 dBZ/km at 2 and 5 kilometers, which is the likely position of an updraft-downdraft interface. Isolated storm cells from four days during TOGA COARE were analyzed. Mean vertical profiles of radar reflectivity (VPRR) were constructed; these profiles were compared with other VPRRs from other tropical oceanic storms, and tropical and mid latitude continental storms. Reflectivity lapse rates were found to be effective in discriminating between lightning and non-lightning storms. A connection between the amount of lightning flashes and characteristics of VPRRs was examined. It was found that lightning and non-lightning storm cell VPRRs were associated with lower reflectivity lapse rates than ever previously been found for tropical oceanic storms. Reflectivity lapse rate of the mean profiles of lightning storm cells was 3.32 dBZ/km versus the 4.33 dBZ/km found for non-lightning storms. Lightning storms had reflectivity lapse rates similar to 3.5 dBZ/km lapse rate of tropical continental storms studied by Zipser and Lutz (I 994). Lapse rates of non-lightning cells significantly exceeded lapse rates from other cells in different tropical oceanic regimes.

Barnaby, Stephen Andrew

1995-01-01T23:59:59.000Z

223

The convective structures associated with cloud-to-ground lightning in TOGA COARE Mesoscale Convective Systems  

E-Print Network (OSTI)

The TOGA COARE experiment was carried out in the western Pacific warm pool region from November 1992 through February 1993. Data from TOGA COARE provide the opportunity for comprehensive studies of tropical oceanic convection. This study seeks to understand more about the structures of tropical mesoscale convective systems in relation to their lightning activity and cold cloud area. The properties of convective cores within the MCSs, as well as their infrared (IR) cloud top temperatures are related to cloud-to-ground lightning. IR and lightning time histories for each case are compared, and the cases ranked by mean flashes per 10000 km2. For selected cases, radar/lightning overlays and vertical profiles of maximum radar reflectivities (VPRRs) are shown in order to describe the convective structures of cells that produced lightning, and also cells that did not produce lightning. IR cloud-top temperature and lightning histories indicate the highly variable nature of the TOGA COARE MCSs. Some MCSs which have very cold cloud-top temperatures have little or no lightning. When a system did produce lightning, more flashes generally occurred during its growth phase. Overall, IR measurements seem to be a poor indicator of lightning due to the lack of a clear relationship between the size of cold cloud-top area and cloud-to-ground flashes. The overall flash rates of the TOGA COARE MCSs support previous work which showed that tropical oceanic convection has an order of magnitude less cloud-to-ground lightning than continental convection. In fact, the highest flash rate for the 13 cases was less than the weakest continental case in a Texas MCS study (Toracinta et al. 1995). Examination of individual VPRR and of all convective VPRR from the 13 cases indicate a pronounced difference between lightning producing and non-lightning producing cells. Lightning producing convective cells have higher radar reflectivity in the 5.0- 8.0 km layer, and a slower decrease with height, both observations indicating larger ice particles in the mixed phase region between 00 C and-200 C.

Restivo, Michael Edward

1995-01-01T23:59:59.000Z

224

Thunderstorm Characteristics of Cloud-to-Ground Lightning at the Kennedy Space Center, Florida: A Study of Lightning Initiation Signatures as Indicated by the WSR-88D  

Science Conference Proceedings (OSTI)

The operational potential for predicting the onset of cloud-to-ground lightning is examined. WSR-88D reflectivity echoes were analyzed for 39 airmass thunderstorms that developed over the NASA Kennedy Space Center, Florida, to determine the best ...

Michael S. Gremillion; Richard E. Orville

1999-10-01T23:59:59.000Z

225

Ensemble Simulation of the Lightning Flash Variability in a 3D Cloud Model with Parameterizations of Cloud Electrification and Lightning Flashes  

Science Conference Proceedings (OSTI)

A series of ensemble simulations were performed to study the statistics of flash characteristics produced by an electrification and lightning scheme in the cloud-resolving model Mso-NH. Here, the electrical variability of two stormsone ...

Jean-Pierre Pinty; Christelle Barthe

2008-01-01T23:59:59.000Z

226

Delimiting Thunderstorm Watch Periods by Real-Time Lightning Location for a Power Utility Company  

Science Conference Proceedings (OSTI)

During times of thunderstorm activity, the power utility serving metropolitan New York enters a potentially costly thunderstorm watch mode of operation which is designed to prevent a major power outage caused by lightning. To evaluate the ...

Vincent P. Idone; Richard E. Orville

1990-03-01T23:59:59.000Z

227

Doppler Radar and Lightning Network Observations of a Severe Outbreak of Tropical Cyclone Tornadoes  

Science Conference Proceedings (OSTI)

Data from a single Weather Surveillance Radar-1988 Doppler (WSR-88D) and the National Lightning Detection Network are used to examine the characteristics of the convective storms that produced a severe tornado outbreak, including three tornadoes ...

Eugene W. McCaul Jr.; Dennis E. Buechler; Steven J. Goodman; Michael Cammarata

2004-07-01T23:59:59.000Z

228

Lightning Casualties and Property Damage in Colorado from 1950 to 1991 Based on Storm Data  

Science Conference Proceedings (OSTI)

Forty-two years of lightning casualty and damage reports in Colorado are summarized. The data are from NOAA's Storm Data, which is compiled monthly by the National Weather Service.

Ral E. Lpez; Ronald L. Holle; Todd A. Heitkamp

1995-03-01T23:59:59.000Z

229

Diurnal and Spatial Variability of Lightning Activity in Northeastern Colorado and Central Florida during the Summer  

Science Conference Proceedings (OSTI)

Lightning location data from northeastern Colorado and central Florida for the summer months of 1983 have been studied to ascertain the diurnal development of spatial distributions of flash frequencies. In both locales, a clearly identifiable ...

Ral E. Lopez; Ronald L. Holle

1986-07-01T23:59:59.000Z

230

Reflectivity, Ice Scattering, and Lightning Characteristics of Hurricane Eyewalls and Rainbands. Part II: Intercomparison of Observations  

Science Conference Proceedings (OSTI)

Part I of this two-part paper treats Tropical Rainfall Measuring Mission (TRMM) radar, passive microwave, and lightning observations in hurricanes individually. This paper (Part II) examines relationships between these parameters (and ...

Daniel J. Cecil; Edward J. Zipser

2002-04-01T23:59:59.000Z

231

Global Positioning System (GPS) Precipitable Water in Forecasting Lightning at Spaceport Canaveral  

Science Conference Proceedings (OSTI)

This paper evaluates the use of precipitable water (PW) from the global positioning system (GPS) in lightning prediction. Additional independent verification of an earlier model is performed. This earlier model used binary logistic regression ...

Kristen Kehrer; Brian Graf; William P. Roeder

2008-04-01T23:59:59.000Z

232

Radar, Passive Microwave, and Lightning Characteristics of Precipitating Systems in the Tropics  

Science Conference Proceedings (OSTI)

The bulk radar reflectivity structures, 85- and 37-GHz brightness temperatures, and lightning characteristics of precipitating systems in tropical Africa, South America, the east Pacific, and west Pacific are documented using data from the ...

E. R. Toracinta; Daniel J. Cecil; Edward J. Zipser; Stephen W. Nesbitt

2002-04-01T23:59:59.000Z

233

Can Lightning Observations be Used as an Indicator of Upper-Tropospheric Water Vapor Variability?  

Science Conference Proceedings (OSTI)

Lightning activity in thunderstorms is closely related to the intensity of vertical updrafts indeep convective clouds that also transport large amounts of moisture into the upper troposphere. Small changes in the amount of upper-tropospheric ...

Colin Price; Mustafa Asfur

2006-03-01T23:59:59.000Z

234

Combining Satellite Infrared and Lightning Information to Estimate Warm Season Convective and Stratiform Rainfall  

Science Conference Proceedings (OSTI)

This paper describes and evaluates a satellite rainfall estimation technique that combines infrared and lightning information to estimate precipitation in deep convective systems. The algorithm is developed and tested using seven years (2002-2008) ...

Weixin Xu; Robert F. Adler; Nai-Yu Wang

235

The Relationships between Network Lightning Surface and Hourly Observations of Thunderstorms  

Science Conference Proceedings (OSTI)

Relationships were established between lightning location data and surface hourly observations of thunderstorms for 132 stations in the northeastern United States. The relationships are based on statistics derived from 2 2 contingency tables ...

Ronald M. Reap; Richard E. Orville

1990-01-01T23:59:59.000Z

236

Cloud-to-Ground Lightning in a Tornadic Storm on 8 May 1986  

Science Conference Proceedings (OSTI)

On 8 May 1986, the National Severe Storms Laboratory (NSSL) collected Doppler radar and lightning ground strike data on a supercell storm that produced three tornadoes, including an F3 tornado in Edmond, Oklahoma, approximately 40 km north of ...

Donald R. MacGorman; Kurt E. Nielsen

1991-07-01T23:59:59.000Z

237

Bias Elimination and Scatter in Lightning Location by the VLF Arrival Time Difference Technique  

Science Conference Proceedings (OSTI)

In the very low frequency (VLF) band lightning flashes are detectable at ranges of several thousand kilometers. Studies of experimental data show that if systematic biases were eliminated from the U.K. Meteorological Office's VLF arrival time ...

Anthony C. L. Lee

1990-10-01T23:59:59.000Z

238

Total Lightning Observations with the New and Improved Los Alamos Sferic Array (LASA)  

Science Conference Proceedings (OSTI)

Since 1998, Los Alamos National Laboratory (LANL) has deployed an array of fast electric field change sensors in New Mexico and Florida in support of LANLs satellite lightning observations. In April 2004, all the sensors were significantly ...

Xuan-Min Shao; Mark Stanley; Amy Regan; Jeremiah Harlin; Morrie Pongratz; Michael Stock

2006-10-01T23:59:59.000Z

239

Positive Cloud-to-Ground Lightning in Tornadic Storms and Hailstorms  

Science Conference Proceedings (OSTI)

Although negative ground flashes usually dominate cloud-to-ground lightning activity, positive ground flashes can dominate in some severe storms for periods ranging from 30 min to several hours. Unlike most other types of storms in which positive ...

Donald R. Macgorman; Donald W. Burgess

1994-08-01T23:59:59.000Z

240

Lightning Activity over Land and Sea on the Eastern Coast of the Mediterranean  

Science Conference Proceedings (OSTI)

This paper presents a study of the characteristics of lightning activity during the Cyprus low winter storms over the eastern coast of the Mediterranean. The focus is on changes in the nature of thunderstorms crossing the coastline from the sea ...

Orit Altaratz; Zev Levin; Yoav Yair and; Baruch Ziv

2003-09-01T23:59:59.000Z

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


241

A Lightning Prediction Index that Utilizes GPS Integrated Precipitable Water Vapor  

Science Conference Proceedings (OSTI)

The primary weather forecast challenge at the Cape Canaveral Air Station and Kennedy Space Center is lightning. This paper describes a statistical approach that combines integrated precipitable water vapor (IPWV) data from a global positioning ...

Robert A. Mazany; Steven Businger; Seth I. Gutman; William Roeder

2002-10-01T23:59:59.000Z

242

Three Years of TRMM Precipitation Features. Part I: Radar, Radiometric, and Lightning Characteristics  

Science Conference Proceedings (OSTI)

During its first three years, the Tropical Rainfall Measuring Mission (TRMM) satellite observed nearly six million precipitation features. The population of precipitation features is sorted by lightning flash rate, minimum brightness temperature, ...

Daniel J. Cecil; Steven J. Goodman; Dennis J. Boccippio; Edward J. Zipser; Stephen W. Nesbitt

2005-03-01T23:59:59.000Z

243

The 29 June 2000 Supercell Observed during STEPS. Part II: Lightning and Charge Structure  

Science Conference Proceedings (OSTI)

This second part of a two-part study examines the lightning and charge structure evolution of the 29 June 2000 tornadic supercell observed during the Severe Thunderstorm Electrification and Precipitation Study (STEPS). Data from the National ...

Kyle C. Wiens; Steven A. Rutledge; Sarah A. Tessendorf

2005-12-01T23:59:59.000Z

244

Electrification and Lightning in an Idealized Boundary-Crossing Supercell Simulation of 2 June 1995  

Science Conference Proceedings (OSTI)

A nonhydrostatic cloud model with electrification and lightning processes was utilized to investigate how simulated supercell thunderstorms respond when they move into environments favorable for storm intensification. One model simulation was ...

Alexandre O. Fierro; Matthew S. Gilmore; Edward R. Mansell; Louis J. Wicker; Jerry M. Straka

2006-11-01T23:59:59.000Z

245

Evidence of Tropical Forcing of the 6.5-Day Wave from Lightning Observations over Africa  

Science Conference Proceedings (OSTI)

A study employing observations and climatic reanalysis data is concerned with links between convection and the well-documented 6.5-day stratospheric global wave. Observations from a long-range lightning detection network, known as ZEUS, reveal an ...

Themis G. Chronis; Earle Williams; Emmanouil N. Anagnostou

2007-10-01T23:59:59.000Z

246

Environmental Control of Cloud-to-Ground Lightning Polarity in Severe Storms  

Science Conference Proceedings (OSTI)

In this study, it is hypothesized that the mesoscale environment can indirectly control the cloud-to-ground (CG) lightning polarity of severe storms by directly affecting their structural, dynamical, and microphysical properties, which in turn ...

Lawrence D. Carey; Kurt M. Buffalo

2007-04-01T23:59:59.000Z

247

Performance Assessment of the Optical Transient Detector and Lightning Imaging Sensor. Part I: Predicted Diurnal Variability  

Science Conference Proceedings (OSTI)

Laboratory calibration and observed background radiance data are used to determine the effective sensitivities of the Optical Transient Detector (OTD) and Lightning Imaging Sensor (LIS), as functions of local hour and pixel location within the ...

Dennis J. Boccippio; William J. Koshak; Richard J. Blakeslee

2002-09-01T23:59:59.000Z

248

A Mixed Exponential Distribution Model for Retrieving Ground Flash Fraction from Satellite Lightning Imager Data  

Science Conference Proceedings (OSTI)

A Bayesian inversion method is introduced for retrieving the fraction of ground flashes in a set of flashes observed from a (low earth orbiting or geostationary) satellite lightning imager. The method employs a constrained mixed exponential ...

W. J. Koshak

2011-04-01T23:59:59.000Z

249

Absolute Spectral Irradiance Measurements of Lightning from 375 to 880 nm  

Science Conference Proceedings (OSTI)

A spectrometer-detector optical multichannel analyzer system capable of absolute spectral irradiance measurements has been used to record the time-integrated emissions (150 or 300 ms) from cloud-to-ground lightning. Two detectors, one operating ...

Richard E. Orville; Ronald W. Henderson

1984-11-01T23:59:59.000Z

250

Convective Tendency Images Derived from a Combination of Lightning and Satellite Data  

Science Conference Proceedings (OSTI)

A technique is presented for generating convective tendency products by combining satellite images with observations of cloud-to-ground lightning activity. Rapid scan (5-min) infrared satellite images are used to define the areal distribution of ...

Steven J. Goodman; Dennis E. Buechler; Paul J. Meyer

1988-09-01T23:59:59.000Z

251

Analysis and Prediction of Lightning Strike Distributions Associated with Synoptic Map Types over Florida  

Science Conference Proceedings (OSTI)

The temporal and spatial distributions of lightning activity associated with specific synoptic regimes of low-level wind flow were analyzed as part of an experiment to develop improved statistical thunderstorm forecasts for Florida. The synoptic ...

Ronald M. Reap

1994-08-01T23:59:59.000Z

252

An examination of the relationship between cool season tornadoes and cloud-to-ground lightning flashes  

E-Print Network (OSTI)

The southeast United States is prone to severe weather throughout the year. Despite technological advances, some severe weather events occasionally remain unwarned in this part of the country. Past studies examined the relationship between cloud-to-ground (CG) lightning and warm season severe weather episodes. The present study examined the relationship between cool season tornadoes and CG lightning, with a focus over the southeastern United States, where most cool season tornadoes occur. Data from the Storm Prediction Center and National Lightning Detection Network (NLDN) were used to investigate CG lightning properties within 50 km and one hour before tornado touchdown. This was completed over a period of 13 cool seasons from October 1989 through March 2002. Of 3325 tornado events, 2358 contained at least one NLDN-detectable flash. CG lightning attributes of peak current, multiplicity, and flash density compared well with those of prior warm season lightning research. Overall event frequency appeared to be lower than in the warm season. Almost all Central Plains events were accompanied by at least one NLDN-detectable flash. Up to 70% of tornado events near the Gulf of Mexico and Atlantic coasts contained no NLDN-detectable lightning. Although it is not known why this trend was observed, it is speculated that NLDN detection efficiency and/or storm structure differences may play a role in these observations. Warm season studies have correlated tornadoes with predominantly positive (>50% positive CG lightning), or PPCG storms. Gridded maps showed the greatest percentage and highest frequency of cool season PPCG storms across Kansas and Nebraska, with up to 70% of events associated with PPCG lightning. A secondary, albeit lower, frequency maximum extended 1?° to 2?° inland across Louisiana into North Carolina. This study also subjectively defined a storm with â??enhancedâ? positive cloud-to-ground (EPCG) lightning as one containing >25% positive cloud-to-ground lightning, which corresponds to approximately the 75th percentile of all cool season tornadoes. This has lead to speculation that EPCG criterion may be a better indicator of the possibility of severe weather than the traditional PPCG criterion.

Butts, Douglas Allen, Jr.

2006-12-01T23:59:59.000Z

253

An Approach for Using TFlash to Improve the Lightning Performance of Transmission Lines  

Science Conference Proceedings (OSTI)

TFlash is an advanced transmission line, lightning performance prediction software. TFlash's ability to predict whether lighting will cause a flashover can help in the design of new transmission lines and help modify existing transmission lines to improve performance. However, to make full use of the software, it is important for the user to have an understanding of the parameters that influence the lightning performance of the line. This report highlights the parameters and shows their influence by mean...

2009-12-07T23:59:59.000Z

254

WISDOM-II: a large in silico docking effort for finding novel hits against malaria using computational grid infrastructure  

Science Conference Proceedings (OSTI)

After having deployed a first data challenge on malaria and a second one on avian flu, respectively in summer 2005 and spring 2006, we are demonstrating here again how efficiently the computational grids can be used to produce massive docking data at ... Keywords: EGEE grid infrastructure, Malaria, computational grid, docking

Vinod Kumar Kasam; Jean Salzemann; Vincent Breton; Nicolas Jacq

2007-06-01T23:59:59.000Z

255

Gamma-ray bursts and other sources of giant lightning discharges in protoplanetary systems  

E-Print Network (OSTI)

Lightning in the solar nebula is considered to be one of the probable sources for producing the chondrules that are found in meteorites. Gamma-ray bursts (GRBs) provide a large flux of gamma-rays that Compton scatter and create a charge separation in the gas because the electrons are displaced from the positive ions. The electric field easily exceeds the breakdown value of ~1 V m^-1 over distances of order 0.1 AU. The energy in a giant lightning discharge exceeds a terrestrial lightning flash by a factor of ~10^12. The predicted post-burst emission of gamma-rays from accretion into the newly formed black hole or spin-down of the magnetar is sufficiently intense to cause a lightning storm in the nebula that lasts for days and is more probable than the GRB because the radiation is beamed into a larger solid angle. The giant outbursts from nearby soft gamma-ray repeater sources (SGRs) are also capable of causing giant lightning discharges. The total amount of chondrules produced is in reasonable agreement with the observations of meteorites. Furthermore in the case of GRBs most chondrules were produced in a few major melting events by nearby GRBs and lightning occurred at effectively the same time over the whole nebula, and provide accurate time markers to the formation of chondrules and evolution of the solar nebula. This model provides a reasonable explanation for the delay between the formation of calcium aluminium inclusions (CAIs) and chondrules.

B. McBreen; E. Winston; S. McBreen; L. Hanlon

2005-02-01T23:59:59.000Z

256

Combined Satellite- and Surface-Based Estimation of the IntracloudCloud-to-Ground Lightning Ratio over the Continental United States  

Science Conference Proceedings (OSTI)

Four years of observations from the NASA Optical Transient Detector and Global Atmospherics National Lightning Detection Network are combined to determine the geographic distribution of the climatological intracloudcloud-to-ground (CG) lightning ...

Dennis J. Boccippio; Kenneth L. Cummins; Hugh J. Christian; Steven J. Goodman

2001-01-01T23:59:59.000Z

257

The Spatial and Temporal Distribution of Lightning Strikes and Their Relationship with Vegetation Type, Elevation, and Fire Scars in the Northern Territory  

Science Conference Proceedings (OSTI)

In this paper the authors explore the spatial and temporal patterns of lightning strikes in northern Australia for the first time. In particular, the possible relationships between lightning strikes and elevation, vegetation type, and fire scars (...

Musa Kilinc; Jason Beringer

2007-04-01T23:59:59.000Z

258

A Novel Shape Complementarity Scoring Function for Protein-Protein Docking  

E-Print Network (OSTI)

been a wealth of research on protein-protein docking, described in several reviews.13­19 Predictive: Zhiping Weng, Dept. of Biomedical Engineer- ing, Boston University, 44 Cummington Street, Boston, MA 02215 are composed of the same number of receptor atoms (top; dark disks) and ligand atoms (bottom; light disks

Weng, Zhiping

259

Conceptual study on Flexible Guidance and Docking system for ITER Remote Handling  

E-Print Network (OSTI)

1 Conceptual study on Flexible Guidance and Docking system for ITER Remote Handling Transport Cask divertors and blanket modules from the Tokamak Building to the Hot Cell Building o Addressed topics for ITER Remote Handling Transport Cask o Publications from IST team: · Isabel Ribeiro, Pedro Lima, Pedro

Ribeiro,Isabel

260

The warning time for cloud-to-ground lightning in isolated, ordinary thunderstorms over Houston, Texas  

E-Print Network (OSTI)

Lightning detection over Houston, Texas is possible with the Lightning Detection and Ranging (LDAR-II) network and the National Lightning Detection Network (NLDN). A comparison of the two datasets in conjunction with 37 isolated, ordinary thunderstorms reveals a time separation of 3.1 minutes between the first detected Very High Frequency (VHF) source (i.e. first intracloud discharge) and the first cloud-toground (CG) lightning flash. This CG warning time is increased to 16.1 minutes when using the radar-defined criterion of when the 30-dBZ contour first reaches the -10C isotherm level. Several attempts were made to establish a similar characteristic that could be used to forewarn the occurrence of the final CG in this storm type. Based on the average radar characteristics during the last CG flash in each thunderstorm case, CG activity comes to an end when the 45-dBZ echo falls below the -10C isotherm. Detection efficiencies that remain slightly less than perfect for each network may have allowed for some error when analyzing VHF sources and ground flashes for each convective case. Exhibiting this possible error, four cases actually recorded a greater number of CG flashes than intracloud flashes, which is contrary to typical lightning characteristics. Future studies hope to increase the number of thunderstorm cases to analyze as the LDAR network continues to observe more lightning events. Also, similar approaches could be implemented in differing geographic regions of the country to observe if these lightning characteristics vary depending on latitude, longitude, or climate.

Clements, Nathan Chase

2007-12-01T23:59:59.000Z

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


261

Pulse trains that are characteristic of preliminary breakdown in cloud-to-ground lightning but are not followed by return stroke pulses  

E-Print Network (OSTI)

findings for lightning locating systems such as the U.S. National Light- ning Detection Network (NLDN-to-ground discharges by lightning locating systems such as the U.S. National Lightning Detection Network. Citation: Nag measuring system included a cir- cular flat plate antenna followed by an integrator and a unity gain, high

Slatton, Clint

262

Warm Season Lightning Distributions over the Northern Gulf of Mexico Coast and Their Relation to Synoptic-Scale and Mesoscale Environments  

Science Conference Proceedings (OSTI)

Cloud-to-ground lightning data from the National Lightning Detection Network are used to create a warm season (MaySeptember) lightning climatology for the northern Gulf of Mexico coast for the 14-yr period 19892002. Each day is placed into one ...

Jessica R. Smith; Henry E. Fuelberg; Andrew I. Watson

2005-08-01T23:59:59.000Z

263

A Comparison of WSR-88D Reflectivities, SSM/I Brightness Temperatures, and Lightning for Mesoseale Convective Systems in Texas. Part II. SSM/I Brightness Temperatures and Lightning  

Science Conference Proceedings (OSTI)

This study examines simultaneous SSM/I and lightning data for a sample of nine mesoscale convective systems (MCS) near League City, Texas. Comparison of lightning files of varying sizes from 2 to 30 min revealed that the 10-min interval ...

Karen I. Mohr; Richard Toracinta; Edward J. Zipser; Richard E. Orville

1996-06-01T23:59:59.000Z

264

A Comparison Of WSR-88D Reflectivities, SSM/I Brightness Temperatures, and Lightning for Mesoscale Convective Systems in Texas. Part I: Radar Reflectivity and Lightning  

Science Conference Proceedings (OSTI)

This is the first part of a two part study. Part I compares radar data from the League City, Texas, WSR-88D and cloud-to-ground (CG) lightning data for a set of eight mesoscale convective systems (MCSs), which occur at various stages of ...

E. Richard Toracinta; Karen I. Mohr; Edward J. Zipser; Richard E. Orville

1996-06-01T23:59:59.000Z

265

Thunderstorm lightning and radar characteristics: insights on electrification and severe weather forecasting  

E-Print Network (OSTI)

Total lightning mapping, along with radar and NLDN cloud-to-ground lightning data, can be used to diagnose the severity of a storm. Analysis of the 13 October 2001 supercell event (Dallas-Fort Worth, Texas), some supercells of which were tornadic, shows that LDAR II lightning source heights (quartile, median, and 95th percentile heights) increased as the storms intensified. Most of the total lightning occurred where reflectivity cores extended upwards and within regions of reflectivity gradient rather than in reflectivity cores. A total lightning hole was associated with an intense, nontornadic supercell on 6 April 2003. This feature was nonexistent from all supercells analyzed during the 13 October case. During tornadogenesis, the radar and LDAR II data indicated updraft weakening. The height of the 30 dBZ radar top began to descend approximately 10 minutes (2 volume scans) before tornado touchdown in one storm. Total lightning and CG flash rates decreased by up to a factor of 5 to a minimum during an F2 tornado touchdown associated with this storm. LDAR II source heights all showed descent by 2-4 km during a 25 minute period prior to and during this tornado touchdown. This drastic trend of decreasing source heights was observed in two tornadic storms prior to and during tornado touchdown, but did not occur in non-tornadic supercells, suggesting that these parameters can be useful to forecasters. These observations agree with tornadogenesis theory that an updraft weakens and the mesocyclone can become divided (composed of both updraft and downdraft) when a storm becomes tornadic. LDAR II source density contours were comma-shaped in association with severe wind events within mesoscale convective systems (MCSs) on 13 October 2001 and 27 May 2002. This signature is similar to the radar reflectivity bow echo. Consistent relationships between severe weather, radar and lightning storm characteristics (i.e., lightning heights) were not found for cells within MCSs as was the case for supercells. Cell interactions within MCSs are believed to weaken these relationships as reflectivity and lightning from nearby storms contaminate the cells of interest. It is also more difficult to clearly define a cell within an MCS.

Steiger, Scott Michael

2005-12-01T23:59:59.000Z

266

Laser-Triggered Lightning Laboratory Tests: Preparation for Testing at Mississippi State University High-Voltage Laboratory  

Science Conference Proceedings (OSTI)

Lightning diversion using laser technology could be operationally used in the power industry to protect sensitive facilities such as nuclear power plants and critical substations, control centers, and customer facilities. This report provides results to date and plans for large-scale, high-voltage laboratory testing of laser-triggered lightning technology.

1998-01-15T23:59:59.000Z

267

Comparison of Narrow Bipolar Events with Ordinary Lightning as Proxies for the Microwave-Radiometry Ice-Scattering Signature  

Science Conference Proceedings (OSTI)

The narrow bipolar event (NBE) is a unique lightning discharge that has a short (10 ?s) overall duration, lacks a prior leader phase, and produces too little light output to be visible by optical lightning detectors on satellites. NBEs thus have ...

Abram R. Jacobson; William Boeck; Christopher Jeffery

2007-04-01T23:59:59.000Z

268

Lightning Ground Flashes Associated with Summer 1990 Flash Floods and Streamflow in Tucson, Arizona: An Exploratory Study  

Science Conference Proceedings (OSTI)

Eight flash flood events occurred in the Tucson area of southeastern Arizona during the 1990 summer when a high-resolution lightning detection network was operated in the region. A total of 3479 cloud-to-ground lightning flashes was composited ...

Ronald L. Holle; Shawn P. Bennett

1997-07-01T23:59:59.000Z

269

Surface Wind Convergence as a Short-Term Predictor of Cloud-to-Ground Lightning at Kennedy Space Center  

Science Conference Proceedings (OSTI)

Cloud-to-ground lightning is a significant forecast problem at the Kennedy Space Center (KSC) in Florida. In this study, cloud-to-ground lightning is related in time and space to surface convergence for 244 days during the convective seasons of ...

Andrew I. Watson; Ronald L. Holle; Ral E. Lpez; Robert Ortiz; James R. Nicholson

1991-03-01T23:59:59.000Z

270

Evaluation of Cloud-to-Ground Lightning Data from the Western United States for the 198384 Summer Seasons  

Science Conference Proceedings (OSTI)

Over two million cloud-to-ground lightning strike locations for the period from mid-June through mid-September for the 198384 summer seasons were evaluated to determine the large-scale climatological characteristics of summertime lightning ...

Ronald M. Reap

1986-06-01T23:59:59.000Z

271

The Evolution of Cloud-to-Ground Lightning within a Portion of the 1011 June 1985 Squall Line  

Science Conference Proceedings (OSTI)

The character of cloud-to-ground lightning is examined during the life cycle of a distinct mesoscale segment of the 1011 June 1985 mesoscale convective system (MCS). Three phases of lightning activity are identified and related to both the radar-...

K. E. Nielsen; R. A. Maddox; S. V. Vasiloff

1994-08-01T23:59:59.000Z

272

Coupling measurement and lightning threat assessment report for Pantex Cell 12-44-1  

SciTech Connect

This report is the first of a series that will quantify the lightning threat to the Pantex Plant where high-risk operations occur. More information can be found in the report written by the Lightning Protection Team [1] and Sandia National Laboratory documents. Low-power RF coupling measurements were completed on Cell 12-44-1 in May 1998. These measurements quantify the voltage and current levels that could leak into the cell from possible lightning strike points. Cell 1 is representative of the most ?leaky? class of cells at Pantex because the floor was not intentionally electrically connected to the walls. From the measurement data, linear models were developed. These transfer functions allow us to calculate the effect in the cell from the much higher power lightning threat. Two types of coupling paths were characterized: (1) external ventilation stack to cell interior and (2) cell ceiling to other cell elements. For the maximum lightning threat [2], an estimate of the maximum cell-to-floor voltage is 150 kV. The extrapolated voltage levels at normal working heights are lower. The potential between the air duct and the electro-static ground is estimated to be 4 kV. A secondary goal was to compare results with Sandia as a quality control check. While the estimated maximum ceiling-to-floor voltages are similar, the comparison was limited by high-frequency resonances on the drive wire.

Anderson, R; Ong, M

1998-09-01T23:59:59.000Z

273

Coupling measurement and lightning threat assessment report for Pantex Cell 12-44-1  

SciTech Connect

This report is the first of a series that will quantify the lightning threat to the Pantex Plant where high-risk operations occur. More information can be found in the report written by the Lightning Protection Team [1] and Sandia National Laboratory documents. Low-power RF coupling measurements were completed on Cell 12-44-1 in May 1998. These measurements quantify the voltage and current levels that could leak into the cell from possible lightning strike points. Cell 1 is representative of the most leaky class of cells at Pantex because the floor was not intentionally electrically connected to the walls. From the measurement data, linear models were developed. These transfer functions allow us to calculate the effect in the cell from the much higher power lightning threat. Two types of coupling paths were characterized: (1) external ventilation stack to cell interior and (2) cell ceiling to other cell elements. For the maximum lightning threat [2], an estimate of the maximum cell-to-floor voltage is 150 kV. The extrapolated voltage levels at normal working heights are lower. The potential between the air duct and the electro-static ground is estimated to be 4 kV. A secondary goal was to compare results with Sandia as a quality control check. While the estimated maximum ceiling-to-floor voltages are similar, the comparison was limited by high-frequency resonances on the drive wire.

Anderson, R; Ong, M.

1998-09-01T23:59:59.000Z

274

Protection characteristics of a Faraday cage compromised by lightning burnthrough.  

SciTech Connect

A lightning flash consists of multiple, high-amplitude but short duration return strokes. Between the return strokes is a lower amplitude, continuing current which flows for longer duration. If the walls of a Faraday cage are made of thin enough metal, the continuing current can melt a hole through the metal in a process called burnthrough. A subsequent return stroke can couple energy through this newly-formed hole. This LDRD is a study of the protection provided by a Faraday cage when it has been compromised by burnthrough. We initially repeated some previous experiments and expanded on them in terms of scope and diagnostics to form a knowledge baseline of the coupling phenomena. We then used a combination of experiment, analysis and numerical modeling to study four coupling mechanisms: indirect electric field coupling, indirect magnetic field coupling, conduction through plasma and breakdown through the hole. We discovered voltages higher than those encountered in the previous set of experiments (on the order of several hundreds of volts).

Warne, Larry Kevin; Bystrom, Edward; Jorgenson, Roy Eberhardt; Montoya, Sandra L.; Merewether, Kimball O.; Coats, Rebecca Sue; Martinez, Leonard E.; Jojola, John M.

2012-01-01T23:59:59.000Z

275

A simple approach to improve lightning performance of an uprated substation  

Science Conference Proceedings (OSTI)

This paper presents a simple method to minimize lightning surges entering an uprated or compact substation. A severe lightning stress is caused by a backflashover in close proximity to the substation. Feasibility of uprating is based on the surge arrester technology available at present. However, some aspects of line design offer opportunities in reducing frequency and severity of lightning surges imposed on the substation. The tower surge response adds an inductive overshoot only during the front of the stroke which reduces considerably during the tail. If backflashover does not occur before reflections from adjacent towers arrive, it is unlikely to occur at all. Use of guys and underbuilt ground wires in the limiting distance will produce reflections with larger magnitude and reduce the effective surge impedance of the tower. This would not only reduce backflashover frequency but will also minimize crest and duration of surges entering the substation.

Harrington, R.J.; Mueen, M. [George Washington Univ., Washington, DC (United States). Dept. of Electrical Engineering and Computer Science

1996-07-01T23:59:59.000Z

276

Lightning overvoltage protection of the paddock 362-145 kV Gas-Insulated Substation  

SciTech Connect

Backflashovers close to the Paddiock 362-145 kV Gas-Insulated Substation (GIS) have been analyzed with the Electro-Magnetic Transient Program (EMTP) using a frequency dependent multi-conductor system. The severity of the lightning stroke currents were derived based on recent recordings in the eastern United States. Impacts of corona attenuation and distortion were accounted for using a shunt linear model approach. Turn-up effects of both line insulator flashover voltages and surge arrester protective characteristics were represented based on manufacturer's volt-time curves. Wave shaping effects of substation capacitances (ie., PT's transformers, CCPD's) were also modeled. Results show the importance of various modeling details in determining the overvoltages inside the GIS due to close backflashovers, which are caused by lightning strokes with varying intensity. These results are aimed at better evaluation of lightning protection requirements for GIS protected by metal-oxide surge arresters.

Elahi, H.; Sublich, M. (GE Industry and Utility Sales, Schenectady, NY (US)); Anderson, M.E.; Nelson, B.D. (Wisconsin Power and Light Co., Madison, WI (USA))

1990-01-01T23:59:59.000Z

277

Radar-Derived Forecasts of Cloud-to-Ground Lightning Over Houston, Texas  

E-Print Network (OSTI)

Ten years (1997 - 2006) of summer (June, July, August) daytime (14 - 00 Z) Weather Surveillance Radar - 1988 Doppler data for Houston, TX were examined to determine the best radar-derived lightning forecasting predictors. Convective cells were tracked using a modified version of the Storm Cell Identification and Tracking (SCIT) algorithm and then correlated to cloud-to-ground lightning data from the National Lightning Detection Network (NLDN). Combinations of three radar reflectivity values (30, 35, and 40 dBZ) at four isothermal levels (-10, -15, -20, and updraft -10 degrees C) and a new radar-derived product, vertically integrated ice (VII), were used to optimize a radar-based lightning forecast algorithm. Forecasts were also delineated by range and the number of times a cell was identified and tracked by the modified SCIT algorithm. This study objectively analyzed 65,399 unique cells, and 1,028,510 to find the best lightning forecast criteria. Results show that using 30 dBZ at the -20 degrees C isotherm on cells within 75 km of the radar that have been tracked for at least 2 consecutive scan produces the best forecasts with a critical success index (CSI) of 0.71. The best VII predictor was 0.734 kg m-2 on cells within 75 km of the radar that have been tracked for at least 2 consecutive scans producing a CSI of 0.68. Results of this study further suggest that combining the radar reflectivity and VII methods can result in a more accurate lightning forecast than either method alone.

Mosier, Richard Matthew

2009-12-01T23:59:59.000Z

278

Cloud to ground lightning in tropical cyclone: a study of 34 West Atlantic tropical cyclones from 1986-1996  

E-Print Network (OSTI)

Cloud to ground (CG) lightning characteristics and patterns were investigated for 34 tropical cyclones for the time period 1986 to 1996. Spatial analysis of CG lightning relative to both compass directions and the direction of motion were compiled. Additionally, a radial analysis of this data was performed within both a 300 km radius and a 50 km radius range. Finally, all analyses were stratified into all tropical cyclones and hurricane categories for further consideration. The National Lightning Detection Network recorded 307,593 CG lightning flashes within 300 km of the storms in this study. Negative flashes had an average first stroke peak current of -49 kA and a mean multiplicity of 2.7. Positive flashes occurred at a rate of 5.3 percent, with a mean peak current of 43 kA and 1.2 multiplicity average. Detected CG lightning characteristics changed significantly with a network upgrade in 1994. CG lightning flashes for all tropical cyclones favored the area south and west of the cyclone center. However, taking into account storm relative motion, the majority of flashes shifts to the front and right sections of the storm. Radial plots indicated lightning frequency increased throughout the domain, with a maximum between 270 km and 300 km. Hurricanes, however, indicated flashes occurred most often in the north and east quadrants. When adjusting for storm motion, the activity shifts to the right with some evidence of individual rainbands in the rear section. Radial plots indicated the increased organization of hurricanes, with a weak maxima in the convective eyewall, a minimum in the stratiform area, and a strong maximum in the outer rainbands. Further analysis indicated a higher frequency of CG lightning flashes in hurricanes suggests a state of hurricane strengthening or weakening. However, there was no direct correlation in the observed lightning frequency with a given change in the central pressure of the hurricane.

Coyne, John Michael

2001-01-01T23:59:59.000Z

279

Lightning arrestor connector lead magnesium niobate qualification pellet test procedures.  

SciTech Connect

Enhanced knowledge preservation for DOE DP technical component activities has recently received much attention. As part of this recent knowledge preservation effort, improved documentation of the sample preparation and electrical testing procedures for lead magnesium niobate--lead titanate (PMN/PT) qualification pellets was completed. The qualification pellets are fabricated from the same parent powders used to produce PMN/PT lightning arrestor connector (LAC) granules at HWF&T. In our report, the procedures for fired pellet surface preparation, electrode deposition, electrical testing and data recording are described. The dielectric measurements described in our report are an information only test. Technical reasons for selecting the electrode material, electrode size and geometry are presented. The electrical testing is based on measuring the dielectric constant and dissipation factor of the pellet during cooling from 280 C to 220 C. The most important data are the temperature for which the peak dielectric constant occurs (Curie Point temperature) and the peak dielectric constant magnitude. We determined that the peak dielectric constant for our procedure would be that measured at 1 kHz at the Curie Point. Both the peak dielectric constant and the Curie point parameters provide semi-quantitative information concerning the chemical and microstructural homogeneity of the parent material used for the production of PMN/PT granules for LACs. Finally, we have proposed flag limits for the dielectric data for the pellets. Specifically, if the temperature of the peak dielectric constant falls outside the range of 250 C {+-} 30 C we propose that a flag limit be imposed that will initiate communication between production agency and design agency personnel. If the peak dielectric constant measured falls outside the range 25,000 {+-} 10,000 we also propose that a flag limit be imposed.

Tuohig, W. (Honeywell FM& T, Kansas City, MO); Mahoney, Patrick A.; Tuttle, Bruce Andrew; Wheeler, Jill Susanne

2009-02-01T23:59:59.000Z

280

DNA Record  

Science Conference Proceedings (OSTI)

... 4. Field 18.004: Source agency/SRC 5. Field 18.005: DNA Record Header Information (DRI) ... Field 18.005: DNA Record Header Information (DRI) ...

2010-08-03T23:59:59.000Z

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


281

Lightning Charge Retrievals: Dimensional Reduction, LDAR Constraints, and a First Comparison with LIS Satellite Data  

Science Conference Proceedings (OSTI)

A dimensional reduction (DR) method is introduced for analyzing lightning field changes (?Es) whereby the number of unknowns in a discrete two-charge model is reduced from the standard eight (x, y, z, Q, x?, y?, z?, Q?) to just four (x, y, z, ...

W. J. Koshak; E. P. Krider; N. Murray; D. J. Boccippio

2007-11-01T23:59:59.000Z

282

Seasonal, Regional, and Storm-Scale Variability of Cloud-to-Ground Lightning Characteristics in Florida  

Science Conference Proceedings (OSTI)

Seasonal, regional, and storm-scale variations of cloud-to-ground (CG) lightning characteristics in Florida are presented. Strong positive CG (+CG) and negative CG (?CG) flashes (i.e., having large peak current) are emphasized since they often are ...

Scott D. Rudlosky; Henry E. Fuelberg

2011-06-01T23:59:59.000Z

283

Observations of High Ground Flash Densities of Positive Lightning in Summertime Thunderstorms  

Science Conference Proceedings (OSTI)

Observations of summertime thunderstorms indicate that positive polarity cloud-to-ground lightning activity can occur with rates as high as 67 flashes in 5 min and spatial densities up to 0.60 flashes per square kilometer per hour. All ground ...

Maribeth Stolzenburg

1994-08-01T23:59:59.000Z

284

Relationships between Thunderstorms and Cloud-to-Ground Lightning in the United States  

Science Conference Proceedings (OSTI)

Data from networks of lightning sensors operated during 1986?89 were employed to perform climatic assessments of cloud-to-ground (CG) flashes, and of the relationship between CG flashes and thunder events, as reported at 62 first-order stations. ...

Stanley A. Changnon

1993-01-01T23:59:59.000Z

285

Cloud-to-Ground Lightning throughout the Lifetime of a Severe Storm System in Oklahoma  

Science Conference Proceedings (OSTI)

Cloud-to-ground (CG) lightning data are examined relative to digitized radar data for a storm system that occurred in Oklahoma on 26 May 1985. This system evolved through three stages: 1) two lines of cells, one near the dryline and the other 60 ...

Mark A. Shafer; Donald R. MacGorman; Frederick H. Carr

2000-06-01T23:59:59.000Z

286

A Photoelectric Technique for Measuring Lightning-Channel Propagation Velocities from a Mobile Laboratory  

Science Conference Proceedings (OSTI)

We have developed a device to measure lightning-channel propagation velocities. It consists of eight solid state silicon photodetectors mounted behind precision horizontal slits in the focal plane of a 50-mm lens on a 35-mm camera body. Each ...

Douglas M. Mach; W. David Rust

1989-06-01T23:59:59.000Z

287

Discovery's Final Flight Lightning Signature Could Help Reveal Solar System's Origin  

E-Print Network (OSTI)

of exploration, learning, and fun. The theme, "Explore Our World," gave visitors an opportunity to move objects in the distant uni- verse, as well as here on Earth. At the thermal engineering table, attendees Goddard Updates The Weekly - 2 NASA Exhibits Inspire Maryland Day Explorers - 3 Lightning Signature Could

288

Storms and Lightning Activity in Greece during the Warm Periods of 200306  

Science Conference Proceedings (OSTI)

Lightning activity over Greece during the warm season (MaySeptember) of the years 200306 is investigated in relation to the synoptic meteorological conditions that prevailed in the region. The study is based on the use of cloud-to-ground ...

N. Mazarakis; V. Kotroni; K. Lagouvardos; A. A. Argiriou

2008-12-01T23:59:59.000Z

289

Radar Nowcasting of Cloud-to-Ground Lightning over Houston, Texas  

Science Conference Proceedings (OSTI)

Ten years (19972006) of summer (JuneAugust) daytime (14000000 UTC) Weather Surveillance Radar-1988 Doppler data for Houston, Texas, were examined to determine the best radar-derived predictors of the first cloud-to-ground lightning flash from a ...

Richard M. Mosier; Courtney Schumacher; Richard E. Orville; Lawrence D. Carey

2011-04-01T23:59:59.000Z

290

Spatial and Temporal Patterns of Thunderstorm Events that Produce Cloud-to-Ground Lightning in the Interior Southeastern United States  

Science Conference Proceedings (OSTI)

Cloud-to-ground (CG) lightning data are used in this study to trace the daily patterns of thunderstorms in time and space across the topographically diverse southeastern United States. Four reoccurring patterns of thunderstorms (i.e., local, ...

Mark S. Murphy; Charles E. Konrad II

2005-06-01T23:59:59.000Z

291

Understanding the Relationships between Lightning, Cloud Microphysics, and Airborne Radar-Derived Storm Structure during Hurricane Karl (2010)  

Science Conference Proceedings (OSTI)

This study explores relationships between lightning, cloud microphysics, and tropical cyclone (TC) storm structure in Hurricane Karl (16 September 2010) using data collected by the NASA DC-8 and Global Hawk (GH) aircraft during NASAs Genesis and ...

Brad Reinhart; Henry Fuelberg; Richard Blakeslee; Douglas Mach; Andrew Heymsfield; Aaron Bansemer; Stephen L. Durden; Simone Tanelli; Gerald Heymsfield; Bjorn Lambrigtsen

292

Nocturnal Photographs Taken from a U-2 Airplane Looking Down on Tops of Clouds Illuminated by Lightning  

Science Conference Proceedings (OSTI)

Photographs have been taken at night from an airplane at an altitude of 20 km looking directly down on the tops of thunderclouds illuminated by lightning. The hard, cauliflower-like appearance of the clouds gives evidence that strong convective ...

B. Vonnegut; O. H. Vaughan Jr.; M. Brook

1989-10-01T23:59:59.000Z

293

An Operational System for the Remote Location of Lightning Flashes Using a VLF Arrival Time Difference Technique  

Science Conference Proceedings (OSTI)

An operational system for the remote location of lightning flashes at ranges of thousands of kilometers is presented. The vertical electric fields of VLF radio atmospherics (sferics), together with time data, are observed at a network of just ...

Anthony C. L. Lee

1986-12-01T23:59:59.000Z

294

The Relationship between Precipitation and Lightning in Tropical Island Convection: A C-Band Polarimetric Radar Study  

Science Conference Proceedings (OSTI)

One of the primary scientific objectives of the Maritime Continent Thunderstorm Experiment was to study cloud electrification processes in tropical island convection, in particular, the coupling between ice phase precipitation and lightning ...

Lawrence D. Carey; Steven A. Rutledge

2000-08-01T23:59:59.000Z

295

Anomalous Cloud-to-Ground Lightning in an F5-Tornado-Producing Supercell Thunderstorm on 28 August 1990  

Science Conference Proceedings (OSTI)

An F5 tornado that devastated Plainfield, Illinois, and environs on 28 August 1990, killing 29 people, is shown to be preduced by a thunderstorm characterized by highly anomalous could-to-ground (CG) lightning activity. Unlike typical summertime ...

Anton Seimon

1993-02-01T23:59:59.000Z

296

The Relationship of Lightning Activity with Microwave Brightness Temperatures and Spaceborne Radar Reflectivity Profiles in the Central and Eastern Mediterranean  

Science Conference Proceedings (OSTI)

In this paper, the relationship of lightning activity in the central and eastern Mediterranean with the 85-GHz polarization-corrected temperature (PCT) and radar reflectivity provided by the Tropical Rainfall Measuring Mission (TRMM) satellite is ...

D. K. Katsanos; K. Lagouvardos; V. Kotroni; A. A. Argiriou

2007-11-01T23:59:59.000Z

297

A New Spatial-Scale Decomposition of the Brier Score: Application to the Verification of Lightning Probability Forecasts  

Science Conference Proceedings (OSTI)

A new scale decomposition of the Brier score for the verification of probabilistic forecasts defined on a spatial domain is introduced. The technique is illustrated on the Canadian Meteorological Centre (CMC) lightning probability forecasts. ...

B. Casati; L. J. Wilson

2007-09-01T23:59:59.000Z

298

Global Lightning Variations Caused by Changes in Thunderstorm Flash Rate and by Changes in the Number of Thunderstorms  

Science Conference Proceedings (OSTI)

Global lightning activity is highly variable on many timescales. This variability is attributable to changes in the flash rate per thunderstorm, the number of thunderstorms, or a combination. The Tropical Rainfall Measuring Mission provides ...

E. Williams; K. Rothkin; D. Stevenson; D. Boccippio

2000-12-01T23:59:59.000Z

299

A Method to Identify the Optimal Areal Unit for NLDN Cloud-to-Ground Lightning Flash Data Analysis  

Science Conference Proceedings (OSTI)

Currently, no uniform method exists for determining the optimal areal unit to analyze National Lightning Detection Network (NLDN) data. To address this problem, this paper utilizes the capabilities of modern geographic information systems (GIS) ...

Michael D. Schultz; S. Jeffrey Underwood; Premkrishnan Radhakrishnan

2005-05-01T23:59:59.000Z

300

Intraseasonal Forcing of Convection and Lightning Activity in the Southern Amazon as a Function of Cross-Equatorial Flow  

Science Conference Proceedings (OSTI)

This study focuses on modulation of lightning and convective vertical structure in the southern Amazon as a function of the South American monsoon V index (VI). Four wet seasons (DecemberMarch 19982001) of Tropical Rainfall Measuring Mission (...

Walter A. Petersen; Rong Fu; Mingxuan Chen; Richard Blakeslee

2006-07-01T23:59:59.000Z

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
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301

Characteristic Differences of Rainfall and Cloud-to-Ground Lightning Activity over South Korea during the Summer Monsoon Season  

Science Conference Proceedings (OSTI)

In this paper the characteristic variations of cloud-to-ground (CG) lightning and total precipitation during the Korean summer monsoon (JuneAugust) season have been extensively analyzed for different climate regimes. The data used in this study ...

S. K. Kar; Kyung-Ja Ha

2003-10-01T23:59:59.000Z

302

DNA Extraction  

NLE Websites -- All DOE Office Websites (Extended Search)

DNA Extraction DNA Extraction Being able to extract deoxyribonucleic acid (DNA) is important for a number of reasons. By studying DNA, scientists can identify genetic disorders or diseases, and they can also possibly find cures for them by manipulating or experimenting with this DNA. At the Laboratory, researchers have studied DNA to detect biothreat agents in environmental and forensic samples. Scientists also are studying how human DNA may be destroyed by certain types of electromagnetic waves at certain frequencies. Classroom Activity: This activity is about the extraction of DNA from strawberries. Strawberries are a great fruit to use for this lesson because each student can work on his or her own. Strawberries are recommended because they yield more DNA than any other fruit. Strawberries are octoploid, which means that they have eight copies of each

303

Environmental control of cloud-to-ground lightning polarity in severe storms  

E-Print Network (OSTI)

In this study, it is hypothesized that the mesoscale environment can indirectly control the cloud-to-ground (CG) lightning polarity of severe storms by directly affecting their structural, dynamical, and microphysical properties, which in turn directly control cloud electrification and CG flash polarity. A more specific hypothesis, which has been supported by past observational and laboratory charging studies, suggests that broad, strong updrafts and associated large liquid water contents in severe storms lead to enhanced positive charging of graupel and hail via the noninductive charging mechanism, the generation of an inverted charge structure, and increased positive CG lightning production. The corollary is that environmental conditions favoring these kinematic and microphysical characteristics should support severe storms generating an anomalously high (> 25%) percentage of positive CG lightning (i.e., positive storms), while environmental conditions relatively less favorable should sustain storms characterized by a typical (? 25%) percentage of positive CG lightning (i.e., negative storms). Forty-eight inflow proximity soundings were analyzed to characterize the environments of nine distinct mesoscale regions of severe storms (four positive and five negative) on six days during May June 2002 over the central United States. This analysis clearly demonstrated significant and systematic differences in the mesoscale environments of positive and negative storms, which were consistent with the stated hypothesis. When compared to negative storms, positive storms occurred in environments associated with a drier low to midtroposphere, higher cloud base height, smaller warm cloud depth, stronger conditional instability, larger 0-3 km AGL wind shear, stronger 0-2 km AGL storm-relative wind speed, and larger buoyancy in the mixed-phase zone, at a statistically significant level. Differences in the warm cloud depth of positive and negative storms were by far the most dramatic, suggesting an important role for this parameter in controlling CG lightning polarity. Subjective visual inspection of radar imagery revealed no strong relationship between convective mode and CG lightning polarity, and also illustrated that positive and negative severe storms can be equally intense.

Buffalo, Kurt Matthew

2007-12-01T23:59:59.000Z

304

Environmental control of cloud-to-ground lightning polarity in severe storms  

E-Print Network (OSTI)

In this study, it is hypothesized that the mesoscale environment can indirectly control the cloud-to-ground (CG) lightning polarity of severe storms by directly affecting their structural, dynamical, and microphysical properties, which in turn directly control cloud electrification and CG flash polarity. A more specific hypothesis, which has been supported by past observational and laboratory charging studies, suggests that broad, strong updrafts and associated large liquid water contents in severe storms lead to enhanced positive charging of graupel and hail via the noninductive charging mechanism, the generation of an inverted charge structure, and increased positive CG lightning production. The corollary is that environmental conditions favoring these kinematic and microphysical characteristics should support severe storms generating an anomalously high (> 25%) percentage of positive CG lightning (i.e., positive storms), while environmental conditions relatively less favorable should sustain storms characterized by a typical (? 25%) percentage of positive CG lightning (i.e., negative storms). Forty-eight inflow proximity soundings were analyzed to characterize the environments of nine distinct mesoscale regions of severe storms (four positive and five negative) on six days during May - June 2002 over the central United States. This analysis clearly demonstrated significant and systematic differences in the mesoscale environments of positive and negative storms, which were consistent with the stated hypothesis. When compared to negative storms, positive storms occurred in environments associated with a drier low to midtroposphere, higher cloud base height, smaller warm cloud depth, stronger conditional instability, larger 0-3 km AGL wind shear, stronger 0-2 km AGL storm-relative wind speed, and larger buoyancy in the mixed-phase zone, at a statistically significant level. Differences in the warm cloud depth of positive and negative storms were by far the most dramatic, suggesting an important role for this parameter in controlling CG lightning polarity. Subjective visual inspection of radar imagery revealed no strong relationship between convective mode and CG lightning polarity, and also illustrated that positive and negative severe storms can be equally intense.

Buffalo, Kurt Matthew

2007-12-01T23:59:59.000Z

305

DNA Biometrics  

Science Conference Proceedings (OSTI)

... presentation at Advances in Forensic DNA Analysis workshop held at the American Academy of Forensic Sciences meeting (Seattle, WA), February ...

2013-03-15T23:59:59.000Z

306

Forensic DNA:  

Science Conference Proceedings (OSTI)

... January 17, 2008 Press Release From Mayor Bloomberg's STATE OF THE CITY ADDRESS Efforts towards Portable/Mobile DNA Devices ...

2008-09-29T23:59:59.000Z

307

New Revelation of Lightning Ball Observation and Proposal for a Nuclear Reactor Fusion Experiment  

E-Print Network (OSTI)

In this paper, the author brings further details regarding his Lightning Ball observation that were not mentioned in the first one (Ref.1-2). Additionally, he goes more into detail as the three forces that are necessary to allow the residual crescent form the hydrodynamic vortex ring to shrink into a sphere.Further topics are the similarities and analogies between the Lightning Ball formation's theory and the presently undertaken Tokamak-Stellarator-Spheromak fusion reactor experiments. A new theory and its experimental realisation are proposed as to make the shrinking of the hot plasma of reactors into a ball possible by means of the so called long range electromagnetic forces. In this way,the fusion ignition temperature could possibly atteined.

Tar, Domokos

2009-01-01T23:59:59.000Z

308

New Revelation of Lightning Ball Observation and Proposal for a Nuclear Reactor Fusion Experiment  

E-Print Network (OSTI)

In this paper, the author brings further details regarding his Lightning Ball observation that were not mentioned in the first one (Ref.1-2). Additionally, he goes more into detail as the three forces that are necessary to allow the residual crescent form the hydrodynamic vortex ring to shrink into a sphere.Further topics are the similarities and analogies between the Lightning Ball formation's theory and the presently undertaken Tokamak-Stellarator-Spheromak fusion reactor experiments. A new theory and its experimental realisation are proposed as to make the shrinking of the hot plasma of reactors into a ball possible by means of the so called long range electromagnetic forces. In this way,the fusion ignition temperature could possibly atteined.

Domokos Tar

2009-10-12T23:59:59.000Z

309

VLF and LF signatures of mesospheric/lower ionospheric response to lightning discharges  

SciTech Connect

New evidence is presented of disturbances of the electrical conductivity of the nighttime mesosphere and the lower ionosphere in association with lightning discharges. In addition to extensive documentation of the characteristics of a class of event heretofore referred to as early/fast VLF events [Inan et al.], this data reveal a new feature of these events, consisting of a postonset peak that typically lasts for 1-2 s. The authors also report the observation of short-duration VLF or LF perturbation, in which the amplitude of the subionospheric signal exhibits a sudden change within 20 ms of the causative lightning discharge, and recovers back to its original level in < 3 s. These short-duration events have characteristics similiar to the previously observed rapid onset, rapid decay VLF signatures [Dowden et al.]. Both the typical and rapidly recovering events are observed primarily when the causative lightning discharge is within {+-}50 km of the VLF or LF great circle propagation path, indicating that the scattering from the localized disturbance is highly collimated in the forward direction. The latter in turn implies that for the parameters in hand, the transverse extent of the disturbance must be at least {approximately} 100-150 km. The measured VLF signatures are compared with the predictions of a three-dimensional model of subionospheric VLF propogation and scattering in the presence of localized ionospheric disturbances produced by electromagnetic impulses and quasi-electrostatic (QE) fields produced by lightning discharges. The rapidly recovering or short-duration events are consistent with the heating of the ambient electrons by quasi-static electric fields, in cases when heating is not intense enough to exceed the attachment or ionization thresholds. When no significant electron density changes occur, the conductivity changes due to heating alone last only as long as the QE fields, typically less than a few seconds. 29 refs., 12 figs.

Inan, U.S.; Slingeland, A.; Pasko, V.P. [Stanford Univ., Stanford, CA (United States); Rodriguez, J.V. [Phillips Laboratory, Hanscom Air Force Base, MA (United States)

1996-03-01T23:59:59.000Z

310

A Ball Lightning Model as a Possible Explanation of Recently Reported Cavity Lights  

SciTech Connect

The salient features of cavity lights, in particular, mobile luminous objects (MLO's), as have been experimentally observed in superconducting accelerator cavities, are summarized. A model based upon standard electromagnetic interactions between a small particle and the 1.5 GHz cavity excitation field is described. This model can explain some features of these data, in particular, the existence of particle orbits without wall contact. While this result is an important success for the model, it is detailed why the model as it stands is incomplete. It is argued that no avenues for a suitable extension of the model through established physics appear evident, which motivates an investigation of a model based upon a more exotic object, ball lightning. As discussed, further motivation derives from the fact that there are significant similarities in many of the qualitative features of ball lightning and MLO's, even though they appear in quite different circumstances and differ in scale by orders of magnitude. The ball lightning model, which incorporates electromagnetic charges and currents, is based on a symmetrized set of Maxwell's equations in which the electromagnetic sources and fields are characterized by a process called dyality rotation. It is shown that a consistent mathematical description of dyality rotation as a physical process can be achieved by adding suitable (phenomenological) current terms to supplement the usual current terms in the symmetrized Maxwell's equations. These currents, which enable the conservation of electric and magnetic charge, are called vacuum currents. It is shown that the proposed ball lightning model offers a good qualitative explanation of the perplexing aspects of the MLO data. Avenues for further study are indicated.

Fryberger, David; /SLAC

2009-08-04T23:59:59.000Z

311

Lightning-induced coupling of the radiation belts to geomagnetically conjugate ionospheric regions  

SciTech Connect

Very low frequency (VLF) radio observations in Antarctica and North America provide the first evidence that bursts of energetic electrons from the earth's radiation belts commonly precipitate into geomagnetically conjugate ionospheric regions in response to lightning. The electrons, with energies ranging from tens of keV to over one MeV, appear to be scattered out of their otherwise stable trap in the earth's magnetic field by magnetospheric interactions with a regularly observed class of transient, lightning-generated VLF radio waves known as ducted whistlers. The precipitating electrons ionize atmospheric molecules at altitudes between 40 and 90 km, creating transient enhancements of ionization levels in conjugate locations. These ionospheric disturbances can be detected by their characteristic perturbations, sometimes called 'Trimpi events,' of the amplitude and phase of VLF transmitter signals propagating subionospherically within 200 to 250 km of the disturbed areas. The first detailed, one-to-one comparison of such signal perturbations, monitored in conjugate regions, with the multipath structure, arrival azimuths, and predicted electron scattering of simultaneously observed ducted whistlers suggests that every ducted whistler precipitates bursts of radiation belt electrons. If so, the estimated rate at which ducted whistlers contribute to radiation belt losses is comparable to that predicted for plasmaspheric hiss, a different class of magnetospheric wave that is often considered to control the structure of the belts. Lightning could therefore play a significant role in the maintenance of radiation belt equilibrium.

Burgess, W.C.

1993-01-01T23:59:59.000Z

312

Transient performance of substation grounding systems subjected to lightning and similar surge currents  

SciTech Connect

The frequency and time domain performance of a typical substation grounding system subjected to a lightning strike is described and discussed. The computed scalar potentials, electric fields and magnetic fields are presented graphically as a function of spatial coordinate, as a function of time and as a function of both. It is believed that these detailed results have not been published before. First, a double exponential lightning surge current is injected into the center of a square ground grid. This same surge current is then injected at one corner of the grid. The response of the grounding system to the frequency domain electromagnetic spectrum of this signal is computed by a frequency domain electromagnetic field analysis software package. Once all the frequency responses have been computed, the temporal and spatial distributions of the electromagnetic fields inside and near the substation are obtained by an inverse Fourier transformation of all these responses. The results are compared with some well known low frequency results and to recent published work in this area. These results indicate that the performance of the grounding system is significantly dependent on frequency and on the point of impact of the lightning strike.

Xiong, W.; Dawalibi, F.P. (Safe Engineering Services Technologies Ltd., Montreal, Quebec (Canada))

1994-07-01T23:59:59.000Z

313

A classification of Texas thunderstorms according to their cloud-to-ground lightning characteristics during spring 1993  

E-Print Network (OSTI)

The National Lightning Detection Network detected more than 800 000 cloud-to-ground lightning flashes from 119 storm systems which affected Texas during April-June 1993. This study attempted to characterize these flashes over the entire three-month period, then classify the storms according to their lightning characteristics, relative to their type, geographical location, and maximum echo height. Examination of the flashes over the entire period found that the highest ground flash densities within the study region (26.50-34.5" N, 102'-930 W) were in southeastern Texas. The flashes lowering negative charge to ground had a mean multiplicity of 2.91 strokes and median first-return stroke peak current of 32.1 kiloamperes (kA). Six percent of the flashes were positive; the positive flashes exhibited a mean multiplicity of 1.43 and a median peak current of 36.6 kA. Relating the data to storm type revealed that frontal storm lightning was 2-3 times more positive than observed in airmass storms. Negative lightning in airmass storms had slightly higher mean multiplicities and peak currents than in frontal storms, while frontal storms had higher positive flash multiplicities and peak current values. Analysis by storm location showed that inland storm lightning flashes were more than twice as positive as those in coastal storms, but coastal storms had lower multiplicities and higher peak currents (both polarities) than observed in inland storms. Comparison of lightning characteristics to maximum echo height found a positive correlation between flash rates and echo heights, a negative correlation between percent positive rates and echo heights, a positive correlation between negative flash multiplicities and echo heights, and a negative correlation between positive Flash peak currents and echo heights. Negative flash peak currents remained relatively constant with increasing echo heights.

McMillan, Stephen Randall

1995-01-01T23:59:59.000Z

314

Radar, satellite, and lightning characteristics of select mesoscale convective systems in Texas  

E-Print Network (OSTI)

This study compares radar data from the League City, Texas WSR-88D and cloud-to-ground (CG) lightning data for a set of eight mesoscale convective systems (MCSs) which occur at various stages of development along the upper Texas Gulf Coast. Vertical profiles of radar reflectivity (VPRR) as well as plan views and vertical cross sections are constructed to characterize the structure and relative strength of each MCS. The VPRR are also compared with similar profiles from tropical oceanic MCSs. The data show that in all cases the majority of negative CG lightning flashes are located near high reflectivity convective cores (> 35 dBZ) in the mixed phase region (O'C -20'C). Growing or mature MCSs typically had larger negative flash counts and higher percentages of negative lightning (> 80%) associated with convective cores than MCSs at later stages of their life cycle. Comparison of the median VPRR for the various MCSs showed that although each case had high reflectivity cores (45-55 dBZ) in the lowest 2 to 3 km, the more electrically active MCSs were characterized by smaller reflectivity lapse rates (decrease of reflectivity with height) in mixed phase region than the cores in the remaining systems. Based on existing theories of charge separation, the observation of high negative flash counts coincident with convective cores having small reflectivity lapse rates in the mixed phase region is consistent with the presence of large ice particles aloft. Positive CG flashes were mostly located in low reflectivity (< 30 dBZ near the-10C level) stratiform regions, independent of MCS life cycle stage or VPRR type. Several cases with reports of large hail also had high positive flash densities associated with high reflectivity cores. Devlin et al. (1995) compare 85 GHz brightness temperatures from the Special Sensor Microwave/Imager to lightning data for the same set of MCSs examined in this study. The results using radar and 85 GHz data indicate that the presence of large ice particles aloft is the common linkage between MCSs with lightning, with high radar reflectivity aloft, and large 85 GHz temperature depressions.

Toracinta, Ernest Richard

1995-01-01T23:59:59.000Z

315

Climatological lightning characteristics of the Southern Rocky and Appalachian Mountain chains, a comparison of two distinct mountain effects  

E-Print Network (OSTI)

This study presents a high-resolution lightning climatology for southern portions of both the Rocky Mountains and the Appalachian Mountains. Data from the National Lightning Detection Network (NLDN) are analyzed to produce maps of average annual lightning flash density, positive flash density, percent positive flashes, median peak current, and multiplicity. Three-hourly increments are used to demonstrate the annual average diurnal evolution of flash density. Data are also divided into seasonal averages for the same three-hourly increments to describe the daily evolution of flash density for each of the four seasons: December-January-February, March-April-May, June-July-August, and September-October-November. The flash density analyses reveal opposite mountain-valley effects. In the Rocky Mountains, flash density enhancements occur over and near mountains and flash density minima occur in the valleys. In the Appalachians, the enhancements occur in the valleys, while minimums are noted over the mountains. The eastern edge of the Appalachian lightning suppression is determined to be a result of faster propagation of mountain-initiated convection. Weaker mountain breezes in the Appalachians are theorized to be the catalysts for this. The western edge of the suppression is the cumulative effect of consistent flash density gradients at the Appalachian's western slopes. A theory is presented which links this gradient to observations of high median peak currents. Statistical tests on flash density means show that the Appalachian suppression is significant. Multiple regressions predict lightning flash density from terrain characteristics. Vertical wind and thermodynamic profiles, horizontal temperature differences at summit levels, and average annual precipitation complete the study. From these data, a conceptual model is presented to describe the nature of the lightning evolution in each region, and explain the processes that lead to the end state. This study concludes that the differences between the patterns of lightning characteristics in the Southern Rockies and the Southern Appalachians are the cumulative effects of subtle differences in the diurnal evolution patterns. Furthermore, the Appalachian lightning suppression is a product of lightning propagation and storm evolution, rather than a suppression of convective initiation.

Phillips, Stephen Edward

2001-01-01T23:59:59.000Z

316

A monthly climatological study of cloud-to-ground lightning characteristics throughout the contiguous United States: 1989-1994  

E-Print Network (OSTI)

The National Lightning Detection Network (NLDN) has been in operation since 1989. The NLDN is currently composed of a mixture of magnetic direction finders (DFs) and time-of-arrival (TOA) sensors which locate cloud-to-ground (CG) flashes as well as measure their polarity, peak current, and multiplicity. A monthly climatology for the contiguous United States was created for the six years of data to establish an "average year". Analysis was completed for the mean values and interannual variability of several lightning parameters: ground flash density, positive ground flash density, percentage positive lightning, multiplicity, and median first-stroke peak currents. In addition to these lightning characteristics, correlation between monthly lightning and precipitation was analyzed in different areas throughout the country. The 1989-1994 mean monthly maximum values were analyzed. Maximum monthly ground flash density values ranged from 0.050 km-2 in December along the southern Mississippi Valley to 2.96 km-2 in July in central Florida. Similarly, positive ground flash density values ranged from 0.0068 km-2 in December in eastern Texas to 0.12 km-2 in July in northern Kansas. In the percentage positive lightning field a persistent protrusion, with locally higher values, appeared from Minnesota southwestward into Colorado from April through August. Mean monthly percentage positive lightning was highest in December at 16.0% and lowest in August at 3.0%. The mean monthly negative multiplicity ranged from 2.32 in February to 2.78 in June. The mean monthly positive multiplicity ranged from 1.26 in December to 1.35 in July. Geographically, the monthly maxima for the negative multiplicity field were generally found along the Gulf Coastal states, especially in Florida during the summer months. Unlike the negative flashes, the monthly maxima for the positive flashes were generally found in the Midwest. The monthly positive median first-stroke peak current ranged from 39 kA in July-September to 66 kA in December and January. Values for negative flashes ranged from 29 kA in May and June to 43 kA in January. Finally, the correlation between monthly precipitation and lightning flash count was much greater (r 2 >0.6) in the central Great Plains than in the Pacific Northwest and Deep South (r 2 <0.11).

Silver, Alan Corey

1995-01-01T23:59:59.000Z

317

Exploration of the meteorological characteristics leading to the rapid cessation of cloud-to-ground lightning in winter cyclones along the East Coast of the United States  

E-Print Network (OSTI)

Intense winter East Coast cyclones are capable of producing large amounts of cloud-to-ground (CG) lightning. Few studies have thoroughly investigated winter lightning in the eastern United States. A phenomena common to most of these storms is a rapid cessation of CG lightning over land, north of North Carolina. The primary focus of this study was investigating the possible causes of this rapid cessation. A secondary objective was to explore the characteristics of the CG lightning produced by these storms. Seven intense East Coast cyclones from 1990 to 1996 were analyzed. Soundings, lower atmospheric temperature structure and accumulated precipitation were used to examine possible causes for this rapid cessation. Soundings also provided the vertical wind shear and temperatures used for linear regression analysis of the percentage of positive CG flashes. Further exploration of CG lightning characteristics included peak currents and flash densities. The major cause of the CG lightning cessation appears to be related to stability and not a suppression of charging due to cold lower atmospheric temperatures. The warm sector of all but one storm passed out into the Atlantic Ocean before making it north of North Carolina. This eliminated most of the surface instability for convection. Meanwhile, most soundings from Virginia on northward showed that conditions aloft were too stable to support elevated convection. Colder lower atmospheric temperatures were just representative of stable conditions that would not support convection. Unfortunately, heavy precipitation was not a good indicator of the CG lightning activity during these seven storms. Contrary to previous studies of winter thunderstorms, vertical wind shear of the horizontal wind was not a good predictor of the percentage of positive CG flashes. A combination of temperature and vertical wind shear data yielded better results. Finally, maximum peak current values showed a preference for the main CG lightning region south of Virginia. This study shed light on some of the aspens of winter CG lightning during intense East Coast cyclones. However, many more studies are needed within this field.

Demetriades, Nicholas William Snow

1999-01-01T23:59:59.000Z

318

A New Method for the Measurement of the Site Errors of a Lightning Direction-Finder: Description and First Results  

Science Conference Proceedings (OSTI)

One of the major limitations in the use of magnetic direction-finders for locating lightning is the presence of systematic angle errors that are due to the local antenna site. An attempt was made to measure this systematic error, by using a ...

Th Schtte; E. Pisler; S. Israelsson

1987-06-01T23:59:59.000Z

319

IEEE I&CPS Conf., May 7-11, San Antonio, Texas, pp. 147-152 Reliability of Lightning Resistant  

E-Print Network (OSTI)

[1]. This paper presents a compilation of 32 years of historical records for outage causes, duration. Degenhardt, Member, IEEE Abstract An assessment of the 32 year historical reliability of the 13.8 k and cost effective method to reduce lightning caused outages. In addition, comparisons are made between

Tolbert, Leon M.

320

An Eight-Year Lightning Climatology of the Southeast United States Prepared for the 1996 Summer Olympics  

Science Conference Proceedings (OSTI)

The 1996 Summer Olympics will be held in the Atlanta, Georgia, vicinity and several other sites in the southeast United States between 19 July and 4 August 1996. This period coincides with the peak thunderstorm season, so the threat of lightning ...

Andrew I. Watson; Ronald L. Holle

1996-05-01T23:59:59.000Z

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


321

Deaths, Injuries, and Damages from Lightning in the United States in the 1890s in Comparison with the 1990s  

Science Conference Proceedings (OSTI)

A reduction by a factor of 10 in the population-weighted rate of lightning-caused deaths over the last century has been determined in several previous studies. The reasons have been attributed to a number of factors, but none have been quantified ...

Ronald L. Holle; Ral E. Lpez; Bradley C. Navarro

2005-10-01T23:59:59.000Z

322

Evolution of cloud-to-ground lightning characteristics within the convective region of a midlatitude squall line  

E-Print Network (OSTI)

The relationship between storm dynamics and cloud-to-ground (CG) lightning characteristics in the convective region of the 10-11 June 1985 PRE-STORM (Preliminary Regional Experiment for Stormscale Operational and Research Meteorology) squall line has been examined over a period of 49 minutes. Lightning data from the National Severe Storms Laboratory lightning detection network combined with seven dual-Doppler analyses provided a unique dataset in which to study this Mesoscale Convective System (MCS). The mature phase of this system passed through the dual-Doppler analysis domain during a period when the convective region made an apparent transition to a weaker convective state. For the overall system, the peak in flash rates was shown to lag the maximum in the vertical velocity by roughly 1 0 to 20 minutes. Cross-sectional analyses revealed numerous examples of this time lag along with a coincidence of descending mid-toupper level reflectivity features and increasing CG flash rates. Along-line dynamical differences in the convective region may have contributed to significant spatial variations in the flash rate. The northern portion of the line featured stronger, more continuous updrafts along with a relative sparseness of lightning strikes. The southern section displayed weaker, but more discrete cell evolution which coincided with a rather uniform and substantial number of CG strikes.

Billingsley, David Brian

1994-01-01T23:59:59.000Z

323

Initial Studies with the Lightning Detector on the C/NOFS Satellite, and Cross Validation with WWLLN  

Science Conference Proceedings (OSTI)

The lightning detector (LD) on the Communications/Navigation Outage Forecast System (C/NOFS) satellite uses a pair of silicon photodiodes, viewing each flank at right angles to the satellite track over an extended field of view. The data product ...

Abram R. Jacobson; Robert H. Holzworth; Michael P. McCarthy; Robert F. Pfaff

2011-11-01T23:59:59.000Z

324

Effect of the Reaction N + NO ? N*2(v = 5) + O on the Production of NO by Lightning  

Science Conference Proceedings (OSTI)

The channel heating model is used to study the NO production by lightning. The reaction N+NO?N*2 (v = 5)+O is included in this model to investigate the effect on NO production. The calculations show significant changes in the NO production ...

Rajesh Kumar; Vir Singh; Jagdish Rai

1994-01-01T23:59:59.000Z

325

An evaluation of lightning flash characteristics using LDAR and NLDN networks with warm season southeast Texas thunderstorms  

E-Print Network (OSTI)

A comparison of flash parameters from the National Lightning Detection Network (NLDN) is made with data obtained from the Houston Lightning Detection and Ranging II (LDAR) network. This research focuses on relating the peak current and number of strokes in a negative flash (multiplicity) of lightning with the spatial extent and mean altitude of three-dimensional lightning in 1407 flashes as mapped by the LDAR network. It is shown that increasing negative multiplicities over the range two through ten exhibit, on average, a higher flash extent with higher multiplicities. Singlestroke flashes have mean heights of nearly 2 km greater. Higher order multiplicities (2 to 10+) were correlated with mean source heights near 8 km. Increasing multiplicity tends to be associated with greater flash extents increasing more horizontally than vertically with a 50% to 70% increase in flash extent. No obvious relationship between peak current and flash extent was observed. Examining peak current and mean height shows that low current flashes (<10kA) exhibit higher mean heights. However, this may be due to intra-cloud only flashes being reported as cloud to ground events by the NLDN. Bipolar flashes do not show much variation with height and flash extent with the exception of negative-first bipolar flashes, which exhibited mean flash extents twice that of other types. Finally, the flash detection efficiency is 99.7% within 60 km of the network center.

Jurecka, Joseph William

2008-08-01T23:59:59.000Z

326

Doppler Radar Signatures of Developing Thunderstorms and Their Potential to Indicate the Onset of Cloud-to-Ground Lightning  

Science Conference Proceedings (OSTI)

The capability of Doppler weather radars to short-term forecast the initiation of thunderstorms and the onset of cloud-to-ground (CG) lightning is examined. Doppler weather radar data from 28 thunderstorms were analyzed from August 1990 in the ...

Kurt D. Hondl; Michael D. Eilts

1994-08-01T23:59:59.000Z

327

Investigating the Potential of Using Radar Echo Reflectivity to Nowcast Cloud-to-Ground Lightning Initiation over Southern Ontario  

Science Conference Proceedings (OSTI)

The potential for using radar echo reflectivity to forecast cloud-to-ground (CG) lightning initiation in the 01-h time frame was investigated in southern Ontario, Canada. The main purpose of this investigation was to determine a reflectivity ...

Y. Helen Yang; Patrick King

2010-08-01T23:59:59.000Z

328

Physical mechanism and numerical simulation of the inception of the lightning upward leader  

Science Conference Proceedings (OSTI)

The upward leader is a key physical process of the leader progression model of lightning shielding. The inception mechanism and criterion of the upward leader need further understanding and clarification. Based on leader discharge theory, this paper proposes the critical electric field intensity of the stable upward leader (CEFISUL) and characterizes it by the valve electric field intensity on the conductor surface, E{sub L}, which is the basis of a new inception criterion for the upward leader. Through numerical simulation under various physical conditions, we verified that E{sub L} is mainly related to the conductor radius, and data fitting yields the mathematical expression of E{sub L}. We further establish a computational model for lightning shielding performance of the transmission lines based on the proposed CEFISUL criterion, which reproduces the shielding failure rate of typical UHV transmission lines. The model-based calculation results agree well with the statistical data from on-site operations, which show the effectiveness and validity of the CEFISUL criterion.

Li Qingmin [Beijing Key Lab of High Voltage and EMC, School of Electric and Electronic Engineering, North China Electric Power University, Beijing 102206 (China) and State Key Lab of Alternate Electrical Power System with Renewable Energy Sources, North China Electric Power University, Beijing 102206 (China); Lu Xinchang; Shi Wei; Zhang Li; Zou Liang; Lou Jie [Shandong Provincial Key Lab of UHV Technology and Gas Discharge, School of Electrical Engineering, Shandong University, Jinan 250061 (China)

2012-12-15T23:59:59.000Z

329

Recovery signatures of lightning-associated VLF perturbations as a measure of the lower ionosphere  

SciTech Connect

A new model of the physical processes associated with subionospheric VLF signal perturbations caused by lightning-induced electron precipitation (LEP) bursts is developed to diagnose the state of the lower ionosphere (e.g., electron number density and rate coefficients for various chemical reactions) on the basis of measurements of VLF recovery signatures. The model accounts for the energy spectrum of the electron bursts precipitated by lightning-generated whistlers, the chemical relaxation of enhanced secondary ionization in the nightime D region due to LEP bursts, and quantitatively treats the resultant effects on propagation of the VLF signal in the Earth-ionosphere waveguide. Application of the model to experimental data obtained for the VLF propagation path from NPM station (Hawaii) to Palmer station (Antarctica) indicates that effective electron detachment rate {gamma}, enhanced secondary ionization profile (e.g., energy content of LEP bursts), as well as the ambient electron density distribution, may be estimated using observed subionospheric VLF recovery signatures. The effective detachment rate was identified as {approximately}10{sup 18} N s{sup {minus}1}, where N is total number density of neutrals. Model indicates in particular that the attachment-detachment processes play the dominant role in recovery of subionospheric VLF signal perturbations on timescales {approximately}100 s, and that the observed perturbations of the NPM-Palmer signal correspond to the LEP bursts consisting of relatively soft (< 250 keV) electrons. 33 refs., 24 figs.

Pasko, V.P.; Inan, U.S. [Stanford Univ., CA (United States)

1994-09-01T23:59:59.000Z

330

Low-level convergence and its role in convective intensity and frequency over the Houston lightning and rainfall anomaly  

E-Print Network (OSTI)

An increase in the amount of lightning and rainfall over the Houston area, compared to the surrounding rural areas, has been well documented in previous studies. The placement of a Shared Mobile Atmospheric Research and Teaching Radar (SMART-R) in the Houston area during the summer season of 2005 presented a unique opportunity to investigate the role of boundary-layer convergence in modulating convective frequency and intensity and, thereby, likely causing the rainfall and lightning anomalies. The role of the urban heat island (UHI) and the sea-breeze, as a source of low-level convergence leading to enhanced convection over Houston, was examined. Hourly average dual-Doppler wind and convergence maps were created on 1 X 1 km grids for an eleven-week period. By using these images along with average lightning, rainfall, and reflectivity for a large Houston-centered domain, it was possible to discern a correlation between low-level convergence and convection. Also, past findings of enhancement in lightning and rainfall over Houston and downwind of Houston were validated. High convergence levels for the Houston area in the mid-morning were followed closely by a peak in convection in the early afternoon. The enhancement of rainfall and lightning over and downwind of downtown was found to be primarily from a large increase in frequency of deep convective events when compared to the surrounding domain. Also, it was found that UHI, rather than sea-breeze, was likely the primary causative mechanism in the development of convection over the Houston area because of the lack of deep convection in areas equally affected by the sea-breeze and the timing of the convection compared to time of peak sea-breeze. An area of weaker enhancement south of Houston, not discussed in previous studies, was found to be present, possibly from the interactions between the bay-breeze off of the Galveston Bay and the seabreeze.

McNear, Veronica Ann

2003-05-01T23:59:59.000Z

331

DNA Activity  

NLE Websites -- All DOE Office Websites (Extended Search)

DNA Activity DNA Activity Name: Sara Location: N/A Country: N/A Date: N/A Question: Is DNA an anion or a cation? I thought since it was negatively charged it was an anion but mt teacher in class today said it was a cation because negatively charged molecules logically migrate to the positively charged plate of the cathode, ie molecules that migrate towards a cathode are cations. Where is the error in my logic or there error in my logic? Replies: DNA is negatively charged due to the phosphate ions present in the ribose-phosphate backbone. It moves towards the positive pole during electrophoresis. The definition kation/anion is confusing because: 1. a cation moves to the cathode 2. the cathode is negative, thus 3. a cation is positive DNA is an anion. The confusion is that a cathode is negative, but a cation is positively charged. For that reason these terms are not generally used in this context.

332

Estimation of Lightning Stroke Peak Current as a Function of Peak Electric Field and the Normalized Amplitude of Signal Strength: Corrections and Improvements  

Science Conference Proceedings (OSTI)

The authors have made connections and improvements to published equations relating the peak current and the peak electric field intensity for return strokes of cloud-to-ground lightning. The original published equations were derived from ...

Y. P. Liaw; D. R. Cook; D. L. Sisterson

1996-06-01T23:59:59.000Z

333

Patterns of Cloud-to-Ground Lightning and Convective Rainfall Associated with Postwildfire Flash Floods and Debris Flows in Complex Terrain of the Western United States  

Science Conference Proceedings (OSTI)

Flash flood and debris flow reports from Storm Data and the U.S. Geological Survey (USGS) are used to investigate the relationship between hazardous hydrological responses, convective rainfall, and cloud-to-ground (CG) lightning flash parameters. ...

S. Jeffrey Underwood; Michael D. Schultz

2004-10-01T23:59:59.000Z

334

Application of a Lightning Data Assimilation Technique in the WRF-ARW Model at Cloud-Resolving Scales for the Tornado Outbreak of 24 May 2011  

Science Conference Proceedings (OSTI)

This study presents the assimilation of total lightning data to help initiate convection at cloud-resolving scales within a numerical weather prediction model. The test case is the 24 May 2011 Oklahoma tornado outbreak, which was characterized by ...

Alexandre O. Fierro; Edward R. Mansell; Conrad L. Ziegler; Donald R. MacGorman

2012-08-01T23:59:59.000Z

335

Evolution of Eyewall Convective Events as Indicated by Intracloud and Cloud-to-Ground Lightning Activity during the Rapid Intensification of Hurricanes Rita and Katrina  

Science Conference Proceedings (OSTI)

Lightning data (cloud-to-ground plus intracloud) obtained from the Los Alamos Sferic Array (LASA) for 2005s Hurricanes Rita and Katrina were analyzed to provide a first insight into the three-dimensional electrical activity of rapidly ...

Alexandre O. Fierro; Xuan-Min Shao; Timothy Hamlin; Jon M. Reisner; Jeremiah Harlin

2011-05-01T23:59:59.000Z

336

Extending the Capabilities of High-Frequency Rainfall Estimation from Geostationary-Based Satellite Infrared via a Network of Long-Range Lightning Observations  

Science Conference Proceedings (OSTI)

An algorithm for real-time precipitation estimation that combines satellite infrared with long-range lightning network observations is developed. The emphasis is on enhancing current capabilities in continuous rainfall monitoring over large ...

Carlos A. Morales; Emmanouil N. Anagnostou

2003-04-01T23:59:59.000Z

337

Toward Development of Improved QPE in Complex Terrain Using Cloud-to-Ground Lightning Data: A Case Study for the 2005 Monsoon in Southern Arizona  

Science Conference Proceedings (OSTI)

The relationship between convective precipitation and cloud-to-ground (CG) lightning is examined over a study area in southwest Arizona and northwest Mexico. Using seasonal-to-daily and hourly time resolution, the National Climatic Data Center (...

Carlos Manuel Minjarez-Sosa; Christopher L. Castro; Kenneth L. Cummins; E. Philip Krider; Julio Waissmann

2012-12-01T23:59:59.000Z

338

Evolution of Cloud-to-Ground Lightning Characteristics and Storm Structure in the Spearman, Texas, Tornadic Supercells of 31 May 1990  

Science Conference Proceedings (OSTI)

On 31 May 1990, four tornadic supercell storms formed sequentially near the intersection of a dryline and an outflow boundary in the northern Texas panhandle. Staccato lightning flashes, which have been hypothesized to be positive ground ...

Howard B. Bluestein; Donald R. MacGorman

1998-06-01T23:59:59.000Z

339

Relationships between tropical cyclone intensity and satellite based indicators of inner core convection: 85 GHz ice scattering signature and lightning  

E-Print Network (OSTI)

A key component in the maintenance and intensification of tropical cyclones is the transverse circulation, which transports mass and momentum and provides latent heat release via inner core convective updrafts. This study examines these updrafts indirectly, using satellite-borne observations of the scattering of upwelling microwave radiation by precipitation sized ice and satellite-borne observations of lightning. The observations are then compared to tropical cyclone intensity and the resulting relationships are assessed. The size of ice particles is tied to updraft strength, in that stronger updrafts produce more supercooled liquid water, leading to larger graupel through riming. Large graupel and a high supercooled liquid water content are necessary ingredients for charge separation, leading to lightning. Various parameters derived from the inner core ice scattering signature are computed for regions encircling hurricanes and typhoons, and observations of lightning activity or inactivity are analyzed. As expected, increasing ice scattering signature is found to correspond to increasing tropical cyclone intensity. However, the highest correlations do not involve indicators of convective intensity, as they were expected to. Instead, the highest correlations with tropical cyclone intensity result from the ice scattering signature parameters most closely associated with the areal extent of at least moderate precipitation rates. Correlations tend to be of the same sign for both present cyclone intensity at the time of the satellite overpass and subsequent intensity change. Correlations are higher for future cyclone intensity than for either of these. These correlations are much higher for the Atlantic and the Eastern North Pacific than for the Western North Pacific. It is hypothesized that processes involved with the most intense or rapidly intensifying tropical cyclones (more commonly found in the Western North Pacific) are responsible for these differences. The lightning observations are more limited than the microwave observations, because the short amount of time in which lightning can be detected may not adequately represent a particular storms electrical activity. These observations reveal no clear relationships between electrical activity and tropical cyclone intensity. In the examination of case studies, the paradoxical situation of much greater lightning activity in rainbands than in eyewalls is noted.

Cecil, Daniel Joseph

1997-01-01T23:59:59.000Z

340

Evolution of vertical drafts and cloud-to-ground lightning within the convective region of a mesoscale convective complex  

E-Print Network (OSTI)

The evolution of the area-averaged vertical velocity within the objectively defined convective region of the 4 June 1985 PRE-STORM (Preliminary Regional Experiment for Stormscale Operational and Research Meteorology-Central Phase) mesoscale convective complex (MCC) was examined over a 100 minute period during the storm's mature stage and compared to that of a squall line. The area-averaged vertical velocity profiles from twenty dual-Doppler analyses indicated significant differences in convective intensity both spatially and temporally. The peak magnitudes of the mid-to-upper level area-averaged vertical velocity were initially comparable to those from a squall line but became much weaker. The distribution of vertical velocities at mid-to-upper levels was consistent with weakening convection as the percentage of weak updrafts and downdrafts grew at the expense of stronger vertical motions. Typically the non-squall MCC had a much larger percentage of strong downdrafts than did the squall line. Horizontal and vertical cross-sections of both vertical velocity and reflectivity confirmed that the MCC was evolving toward a state with fewer and less intense updraft cores while cross-sections of storm relative flow revealed a descending rear-to-front inflow at later analysis times which reached the surface near locations of active convection. The relationship of cloud-to-ground (CG) lightning to the kinematic structure of the MCC was also studied. For mesoscale regions there was a 10-20 minute time lag between the peak convective intensity and the peak CG flash rate. A similar delay was found on the convective scale for some convective cells, however, other apparently similar cells had little CG lightning throughout their evolution. An analysis of the vertical profiles of the vertical velocity, reflectivity and their gradients above CG lightning suggested a preferred vertical structure of these fields for CG lightning. CG lightning occurred beneath greater than average vertical velocities at mid-to-upper levels and greater than average vertical velocity gradients and reflectivity at all levels. CG lightning also occurred beneath greater than average reflectivity gradients at mid and upper-levels but beneath smaller than average reflectivity gradients at low-levels due to its avoidance of the highest reflectivity gradient regions.

Saul, Scott Henry

1995-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


341

Failure analysis of rutile sleeves in MC3080 lightning arrestor connectors.  

Science Conference Proceedings (OSTI)

The purpose of this SAND Report is to document efforts in the extraction and failure analyses of sleeve-style Lightning Arrestor Connectors (LACs). Several MC3080 and MC3079 LACs were recovered from the field and tested as part of the Enhanced Surveillance Campaign. A portion of these LACs failed retesting. Terry Ernest (01733), the LAC Component Engineer, provided eleven MC3080 LACs for evaluation where four of the LACs failed IR/DCW and one failed FRB requirements. The extraction of rutile sleeves from failed LACs was required to determine the source of failure. Rutile sleeves associated with connector function failures were examined for cracks, debris as well as any other anomalies which could have caused the LAC to not function properly. Sleeves that failed FRB or that experienced high FRB exhibited high symmetry, smooth surface, long-flow amicon, and slightly over-sized inside diameter. LACs that failed DCW or IR requirements had rutile sleeves that exhibited breakdown tracks.

Kilgo, Alice C.; Monroe, Saundra L.; Watson, Chad Samuel; Ernest, Terry L.

2006-02-01T23:59:59.000Z

342

Subionospheric VLF imaging of lightning-induced electron precipitation from the magnetosphere  

SciTech Connect

High-resoluton measurements of subionospheric VLF signals at multiple sites are used as a new tool to assess the spatial distribution and occurrence of ionospheric disturbances associated with lightning-induced electron precipitation. Simultaneous observations in California, Saskatchewan, and Quebec of VLF signals from multiple sources allow the monitoring of event activity over a course grid covering the continental US. Association of the observed VLF signal perturbations with lightning is often made on the basis of time correlation with prominent radio atmospherics. Simultaneous observations of individual events on subionospheric paths that cross one another are used to locate the disturbed ionospheric region(s). Absence of perturbations on nearby paths permits assessment of the spatial exstent of the region with a varying degree of accuracy, depending on the distribution of signal paths. In one case distinctly different onset delays (with respect to causative discharges) consistent with predictions of whistler-particle scattering theory were found, and were interpreted as being due to two separate regions separated in L value by {approximately} 0.4 L being excited in individual events. Occurrence statistics over the course of October 1987 exhibit generally higher levels of activity at the lower-latitude end of the 2 {lt} L {lt} 4 range, although it is difficult to separately assess the role of the source ligntning distribution. Occurrence statistics of simultaneous events on crossing paths are consistent with the spatial extent of the disturbed ionospheric regions being less than a few hundred kilometers. The occurrence statistics also suggest that scattering from disturbances located at distances of {gt} 100 km off the great circle paths is not significant.

Inan, U.S.; Knifsend, F.A.; Oh, J. (Stanford Univ., CA (United States))

1990-10-01T23:59:59.000Z

343

Lightning protection requirements of SF/sub 6/ substation determined by hybrid simulator. [115/345 kv station  

SciTech Connect

The development of a hybrid computer simulation which can provide the type and quantity of data necessary for determining the behavior of lightning-caused surges traveling through substations is discussed. Results from a specific application on a 115-kV/345-kV station are presented. The results show that the simulation of SF/sub 6/ substations during lightning-caused surges is ideally accomplished by hybrid computer. The quantity of data for complete substation analysis may be gathered efficiently. In addition, digital graphical summaries provide quick insight into substation behavior. The application of this method to simple configurations has shown the previous assumptions must be carefully reviewed. For example, it is shown by computer simulation and analytical hand calculation that the surges with the steepest wave fronts may not produce the highest substation voltages. It is concluded that this hybrid simulation is an excellent tool in the determination of SF/sub 6/ substation response to lightning-caused surges. (LCL)

Tranen, J.D. (New England Electric System, Westborough, MA); Hedin, R.A.; Minnick, J.J.; Priest, K.W.

1977-01-01T23:59:59.000Z

344

New Mexico/Geothermal | Open Energy Information  

Open Energy Info (EERE)

Mexico/Geothermal Mexico/Geothermal < New Mexico Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Print PDF New Mexico Geothermal General Regulatory Roadmap Geothermal Power Projects Under Development in New Mexico Developer Location Estimated Capacity (MW) Development Phase Geothermal Area Geothermal Region Lightning Dock I Geothermal Project Raser Technologies Inc Lordsburg, New Mexico Phase I - Resource Procurement and Identification Lightning Dock Geothermal Area Rio Grande Rift Geothermal Region Lightning Dock II Geothermal Project Raser Technologies Inc Lordsburg, NV Phase III - Permitting and Initial Development Lightning Dock Geothermal Area Rio Grande Rift Geothermal Region Add a geothermal project. Operational Geothermal Power Plants in New Mexico

345

Geothermal: Sponsored by OSTI -- GRED STUDIES AND DRILLING OF...  

NLE Websites -- All DOE Office Websites (Extended Search)

STATE 2, AMERICULTURE TILAPIA FARM LIGHTNING DOCK KGRA, ANIMAS VALLEY, NM Geothermal Technologies Legacy Collection HelpFAQ | Site Map | Contact Us | Admin Log On Home...

346

Received: 2 April 2009, Revised: 7 July 2009, Accepted: 15 July 2009, Published online in Wiley InterScience: 2009 Library screening by fragment-based docking  

E-Print Network (OSTI)

. Recognit. (2009) Copyright ? 2009 John Wiley & Sons, Ltd. #12;similar to a breadth-first search. (ii to the relevance of plasmepsins as drug targets. We used our fragment-based docking procedure to search-ray structure of the aldehyde peptidic inhibitor benzoyl-Nle-Lys-Arg-Arg-H (PDB code 2fp7) (bottom left). Only

Caflisch, Amedeo

347

Structure of a Fab Complex with the C-terminal fragment of merozoite surface protein-1 of Plasmodium vivax determined by Computational Docking  

Science Conference Proceedings (OSTI)

One current vaccine candidate against Plasmodium vivax, targeting asexual blood stages, is the major merozoite surface protein-1 of P. vivax (PvMSP-1). Vaccine trials with PvMSP-1$_{19}$ and PvMSP-1$_{33}$ have succeeded in protecting monkeys and it ... Keywords: Merozoite surface protein 1, Plasmodium vivax, antibody-antigen complex, docking, malaria vaccine candidate

M. L. Serrano; A. Gauna; H. A. Prez; E. Squitieri; J. D. Medina

2009-12-01T23:59:59.000Z

348

Annual Report: 2011-2012 Storm Season Sampling, Non-Dry Dock Stormwater Monitoring for Puget Sound Naval Shipyard, Bremerton, WA  

SciTech Connect

Annual PSNS non-dry dock storm water monitoring results for 2011-2012 storm season. Included are a brief description of the sampling procedures, storm event information, laboratory methods and data collection, a results and discussion section, and the conclusions and recommendations.

Brandenberger, Jill M.; Metallo, David; Rupert, Brian; Johnston, Robert K.; Gebhart, Christine

2013-07-03T23:59:59.000Z

349

The DNA project - CECM  

E-Print Network (OSTI)

Software-package files: dna6.zip (for Maple 6), dna7.zip (for Maple 7). Contents: DNA routines, Release 1.04 ( last updated Nov/06/2001 ). Changes over...

350

Clinical DNA Online  

Science Conference Proceedings (OSTI)

Welcome to the NIST Clinical DNA Information Resource. ... Future materials have progress updates. General Information. ...

2013-04-26T23:59:59.000Z

351

Quantitative DNA fiber mapping  

DOE Patents (OSTI)

The present invention relates generally to the DNA mapping and sequencing technologies. In particular, the present invention provides enhanced methods and compositions for the physical mapping and positional cloning of genomic DNA. The present invention also provides a useful analytical technique to directly map cloned DNA sequences onto individual stretched DNA molecules.

Gray, Joe W. (San Francisco, CA); Weier, Heinz-Ulrich G. (Oakland, CA)

1998-01-01T23:59:59.000Z

352

SF6 dielectric behavior in a high voltage circuit breaker at low temperature under lightning impulses  

Science Conference Proceedings (OSTI)

This work concerns the study of the dielectric and thermodynamic behavior of SF{sub 6} in a 145 kV circuit breaker subjected to lightning impulse voltages in both polarities at temperatures varying from ambient (20 C) to {minus}45 C. The filling of the circuit breaker with SF{sub 6} is carried out at the reference point: T = 15 C, P = 6 bars abs. and d = 39.6 kg/m{sup 3}. Devices allowing to detect the appearance of local condensation of SF{sub 6} and to identify the breakdown between the circuit-breaker contacts and the flashover outside the circuit-breaker, are presented. The experimental results show a general tendency to a decrease in the U{sub 50%} voltage when the temperature is decreased at constant density (a decrease by about 5% when the temperature was decreased from +20 C to {minus}25 C). Such a variation would not have a significant influence in industrial practice. Even if this variation is very weak, it also appears from the obtained results that the gas density is not the only parameter which has an influence on the electric strength of the gas. However, to compare results at different temperatures, it would be better to make it at constant density and not at constant pressure, to avoid the dependency of the ratio (E/N) on N (where E is the electric strength and N the density). On the other hand, it seems that the presence of the liquid in the circuit breaker is not a penalizing parameter. The distribution of the electric field corresponding to the geometrical configuration of the studied circuit-breaker is also presented.

Buret, F.; Beroual, A. [Ecole Centrale de Lyon, Ecully (France)

1996-01-01T23:59:59.000Z

353

The Life Cycle of Lightning and Severe Weather in a 34 June 1985 PRE-STORM Mesoscale Convective System  

Science Conference Proceedings (OSTI)

Cloud-to-ground lightning flash characteristics of a series of four mesoscale convective systems (MCS) that occurred in Oklahoma and Kansas on 34 June 1985 during the Oklahoma-Kansas Preliminary Regional Experiment for STORM-Central project are ...

Ronald L. Holle; Andrew I. Watson; Ral E. Lpez; Donald R. Macgorman; Robert Ortiz; William D. Otto

1994-08-01T23:59:59.000Z

354

Lightning from Two National Detection Networks Related to Vertically Integrated Liquid and Echo-Top Information from WSR-88D Radar  

Science Conference Proceedings (OSTI)

Two national cloud-to-ground (CG) lightning-detection systems were operating across the contiguous United States during 1993. These two networks are compared to each other and to WSR-88D radar information collected by the Twin Lakes, Oklahoma, ...

Andrew I. Watson; Ronald L. Holle; RaL. E. Lpez

1995-09-01T23:59:59.000Z

355

The Almena, Kansas, Tornadic Storm of 3 June 1999: A Long-Lived Supercell with Very Little Cloud-to-Ground Lightning  

Science Conference Proceedings (OSTI)

The visual, radar, and lightning characteristics of a severe thunderstorm that spawned a large F3 tornado near Almena, Kansas, on 3 June 1999 are documented. The storm is interesting in that it made a transition from a low-precipitation to ...

Eugene W. McCaul Jr.; Dennis E. Buechler; Stephen Hodanish; Steven J. Goodman

2002-02-01T23:59:59.000Z

356

Influences of the Local Environment on Supercell Cloud-to-Ground Lightning, Radar Characteristics, and Severe Weather on 2 June 1995  

Science Conference Proceedings (OSTI)

Radar, cloud-to-ground (CG) lightning characteristics, and storm reports were documented for 20 long-lived supercell thunderstorms that occurred during a 6-h period in the west Texas Panhandle on 23 June 1995. These thunderstorms occurred in ...

Matthew S. Gilmore; Louis J. Wicker

2002-10-01T23:59:59.000Z

357

The Implementation of an Explicit Charging and Discharge Lightning Scheme within the WRF-ARW Model: Benchmark Simulations of a Continental Squall Line, a Tropical Cyclone, and a Winter Storm  

Science Conference Proceedings (OSTI)

This work describes the recent implementation of explicit lightning physics within the Weather Research and Forecasting (WRF) Model. Charging of hydrometeors consists of five distinct noninductive parameterizations, polarization of cloud water, ...

Alexandre O. Fierro; Edward R. Mansell; Donald R. MacGorman; Conrad L. Ziegler

2013-07-01T23:59:59.000Z

358

How scientists use DNA  

NLE Websites -- All DOE Office Websites (Extended Search)

How scientists use DNA Name: Peter and Edmund Age: NA Location: NA Country: NA Date: NA Question: Dear Scientists, We would like to know some ways that scientists use DNA. For...

359

DNA and Biometrics  

Science Conference Proceedings (OSTI)

... January 17, 2008 Press Release From Mayor Bloomberg's STATE OF THE CITY ADDRESS Efforts towards Portable/Mobile DNA Devices ...

2008-06-25T23:59:59.000Z

360

Patterning nanocrystals using DNA  

E-Print Network (OSTI)

nanocrystals. Angewandte Chemie-International Edition, [2]linked by DNA. Angewandte Chemie-International Edition, 39(with proteins. Angewandte Chemie-International Edition, 40(

Williams, Shara Carol

2003-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


361

Controlling DNA Methylation  

NLE Websites -- All DOE Office Websites (Extended Search)

Controlling DNA Methylation Though life on earth is composed of a diverse range of organisms, some with many different types of tissues and cells, all these are encoded by a molecule we call DNA. The information required to build a protein is stored in DNA within the cells. Not all the message in the DNA is used in each cell and not all the message is used all the time. During cell differentiation, the cells become dedicated for their specific function which involves selectively activating some genes and repressing others. Gene regulation is an important event in the developmental biology and the biology of various diseases, but a more complex process. Controlling DNA Methylation Though life on earth is composed of a diverse range of organisms, some with many different types of tissues and cells, all these are encoded by a molecule we call DNA. The information required to build a protein is stored in DNA within the cells. Not all the message in the DNA is used in each cell and not all the message is used all the time. During cell differentiation, the cells become dedicated for their specific function which involves selectively activating some genes and repressing others. Gene regulation is an important event in the developmental biology and the biology of various diseases, but a more complex process. In the bacteria there are distinct enzymes while one is capable of cleaving DNA, the other protects DNA by modification. The complementary function provided by the set of enzymes offers a defense mechanism against the phage infection and DNA invasion. The incoming DNA is cleaved sequence specifically by the class of enzymes called restriction endonuclease (REase). The host DNA is protected by the sequence specific action of matching set of enzymes called the DNA methyltransferase (MTase). The control of the relative activities of the REase and MTase is critical because a reduced ratio of MTase/REase activity would lead to cell death via autorestriction. However too high a ratio would fail to provide protection against invading viral DNA. In addition a separate group of proteins capable of controlling R-M proteins have been identified in various restriction-modification (R-M) systems which are called C proteins (Roberts et al., 2003).

362

DNA's Role with Proteins  

NLE Websites -- All DOE Office Websites (Extended Search)

DNA's Role with Proteins DNA's Role with Proteins Name: Hans Location: N/A Country: N/A Date: N/A Question: Is it sure that the most important information of living cells is stored in the DNA? DNA seems to be nothing more than an inventory of useful proteins and a tool to create those proteins. Could it be that more important operational know how of how these proteins interact to build a living organism is actually located in the rest of the cell? So that the rest of the cell is the most important inheritance, whereas DNA merely takes care of the genetic variation? Replies: DNA is the entire library of protein information for an organism. All seven types of protein. It is true that in developmental stages of an organism that the presence and absences of certain proteins and other chemicals generated by proteins will influence what the DNA in a "particular" cell will express. Hence, you can start out with one cell and end up with a complex organism. You may have heard some of this information with the cloning activities that have been going on lately. All the inheritance comes from the DNA, but what parts of the DNA expression may be dictated by the cells special characteristics developed upon specializing. In that way the liver cells will only do "liver" things and the kidney cells will only do "kidney" things, BUT they use the same DNA information to operate, just a different portion of the same DNA that pertains to their particular "job". If you can convince a cell that it does not have a special job anymore, then you can develop the entire organism from this cell with the right signals; this is what cloning techniques have done!

363

Annual Report: 2010-2011 Storm Season Sampling For NON-DRY DOCK STORMWATER MONITORING FOR PUGET SOUND NAVAL SHIPYARD, BREMERTON, WA  

SciTech Connect

This interim report summarizes the stormwater monitoring conducted for non-dry dock outfalls in both the confined industrial area and the residential areas of Naval Base Kitsap within the Puget Sound Naval Shipyard (referred to as the Shipyard). This includes the collection, analyses, and descriptive statistics for stormwater sampling conducted from November 2010 through April 2011. Seven stormwater basins within the Shipyard were sampled during at least three storm events to characterize non-dry dock stormwater discharges at selected stormwater drains located within the facility. This serves as the Phase I component of the project and Phase II is planned for the 2011-2012 storm season. These data will assist the Navy, USEPA, Ecology and other stakeholders in understanding the nature and condition of stormwater discharges from the Shipyard and inform the permitting process for new outfall discharges. The data from Phase I was compiled with current stormwater data available from the Shipyard, Sinclair/Dyes Inlet watershed, and Puget Sound in order to support technical investigations for the Draft NPDES permit. The permit would require storm event sampling at selected stormwater drains located within the Shipyard. However, the data must be considered on multiple scales to truly understand potential impairments to beneficial uses within Sinclair and Dyes Inlets.

Brandenberger, Jill M.; Metallo, David; Johnston, Robert K.; Gebhardt, Christine; Hsu, Larry

2012-09-01T23:59:59.000Z

364

Quantitive DNA Fiber Mapping  

SciTech Connect

Several hybridization-based methods used to delineate single copy or repeated DNA sequences in larger genomic intervals take advantage of the increased resolution and sensitivity of free chromatin, i.e., chromatin released from interphase cell nuclei. Quantitative DNA fiber mapping (QDFM) differs from the majority of these methods in that it applies FISH to purified, clonal DNA molecules which have been bound with at least one end to a solid substrate. The DNA molecules are then stretched by the action of a receding meniscus at the water-air interface resulting in DNA molecules stretched homogeneously to about 2.3 kb/{micro}m. When non-isotopically, multicolor-labeled probes are hybridized to these stretched DNA fibers, their respective binding sites are visualized in the fluorescence microscope, their relative distance can be measured and converted into kilobase pairs (kb). The QDFM technique has found useful applications ranging from the detection and delineation of deletions or overlap between linked clones to the construction of high-resolution physical maps to studies of stalled DNA replication and transcription.

Lu, Chun-Mei; Wang, Mei; Greulich-Bode, Karin M.; Weier, Jingly F.; Weier, Heinz-Ulli G.

2008-01-28T23:59:59.000Z

365

Three-dimensional modeling of subionospheric VLF propagation in the presence of localized D region perturbations associated with lightning  

SciTech Connect

A theoretical model of single-mode, subionospheric VLF wave propagation in the presence of localized perturbations of the nighttime D region has been developed. Such perturbations could be produced, for example, by lightning-induced electron precipitation associated with a characteristic type of phase or amplitude perturbation in VLF signals known as Trimpi events. The model assumes that the ionospheric perturbation is slowly varying in the horizontal plane and that mode-coupling is therefore negligible, and accounts for (1) effects of perturbations with finite extent in the dimension transverse to the great circle (GC) path between transmitter and receiver, and (2) effects of perturbations which lie off the GC path as well as on it. The formulation used for the numerical calculations depends significantly on the mode refractive index of the ambient Earth-ionosphere waveguide and the mode refractive index in the region of the perturbation. In the calculations values for the mode refractive index are determined from the electron-density-versus-altitude profiles of both the ambient and perturbed ionospheres. Values for changes in the amplitude and phase of a received signal were obtained from the model and compared with amplitude and phase measurements of a VLF signal received at Palmer Station, Antarctica, from the NPM transmitter (23.4 kHz) in Hawaii during energetic electron precipitation events.

Poulsen, W.L.; Bell, T.F.; Inan, U.S. (Stanford Univ., CA (United States))

1990-03-01T23:59:59.000Z

366

DNA polymerase having modified nucleotide binding site for DNA sequencing  

DOE Patents (OSTI)

A modified gene encoding a modified DNA polymerase is disclosed. The modified polymerase incorporates dideoxynucleotides at least 20-fold better compared to the corresponding deoxynucleotides as compared with the corresponding naturally-occurring DNA polymerase. 6 figs.

Tabor, S.; Richardson, C.

1997-03-25T23:59:59.000Z

367

Searching for DNA Lesions: Structural Evidence for Lower- and Higher-Affinity DNA Binding Conformations of Human Alkyladenine DNA Glycosylase  

E-Print Network (OSTI)

To efficiently repair DNA, human alkyladenine DNA glycosylase (AAG) must search the million-fold excess of unmodified DNA bases to find a handful of DNA lesions. Such a search can be facilitated by the ability of glycosylases, ...

Drennan, Catherine L.

368

Generating DNA code word for DNA computing with realtime PCR  

Science Conference Proceedings (OSTI)

A number of DNA computing models to solve mathematical graph problem such as the Hamiltonian Path Problem (HPP), Traveling Salesman Problem (TSP), and the Shortest Path Problem (SPP) have been proposed and demonstrated. Normally, the DNA sequences used ... Keywords: DNA computing, TaqMan probes, real-time PCR

Muhammad Faiz Mohamed Saaid; Zuwairie Ibrahim; Nor Haniza Sarmin

2008-03-01T23:59:59.000Z

369

Natural DNA sequencing by synthesis  

E-Print Network (OSTI)

high throughput genome sequencing by natural DNA synthesis.E.E. , et al. , Genome sequencing by natural DNA synthesis.p. 1304-51. Human Genome Sequencing, C.I. , Finishing the

Roller, Eric E.

2011-01-01T23:59:59.000Z

370

DNA Profiling Standard Reference Materials  

Science Conference Proceedings (OSTI)

... agreement with the NIST Office of Law Enforcement Standards. ... Related Programs and Projects: SRM 2372 - Human DNA Quantitation Standard. ...

2013-03-15T23:59:59.000Z

371

DNA waves and water  

E-Print Network (OSTI)

Some bacterial and viral DNA sequences have been found to induce low frequency electromagnetic waves in high aqueous dilutions. This phenomenon appears to be triggered by the ambient electromagnetic background of very low frequency. We discuss this phenomenon in the framework of quantum field theory. A scheme able to account for the observations is proposed. The reported phenomenon could allow to develop highly sensitive detection systems for chronic bacterial and viral infections.

L. Montagnier; J. Aissa; E. Del Giudice; C. Lavallee; A. Tedeschi; G. Vitiello

2010-12-23T23:59:59.000Z

372

Radiation and viral DNA  

NLE Websites -- All DOE Office Websites (Extended Search)

Radiation and viral DNA Radiation and viral DNA Name: Loretta L Lamb Age: N/A Location: N/A Country: N/A Date: N/A Question: Can viral DNA be changed through exposure to radiation? If so, what type of radiation will do this? Can these irradiated viruses cause changes in the genome of any human cells they may infect? Can these (or any) viruses actually cause cancer, or do they merely act as triggering devices for cancer? Replies: In theory, any nucleic acid (viral or otherwise) can be changed by exposure to many kinds of radiation. Depending on the type of virus, these may then change the human cells that they infect. Although there are many different things that are being implicated in causing cancers, it looks like a fairly common model involves the sequential "knockout" of several human genes. Viruses may be one cause of such gene changes, radiation and other environmental causes may also contribute. Some of these changes may be inherited through families, so it becomes more likely that the environmental factors may happen to "hit" the right places in cells to cause cancers in these families. If you ask something more specific, perhaps I can focus my response a bit more

373

Properties of DnaB helicase in [lambda] DNA replication  

Science Conference Proceedings (OSTI)

A tailed nicked-circle DNA substrate was used to measure the rapid replication fork (RF) movement catalyzed by E. Coli DnaB helicase and DNA polymerase III holoenzyme (pol III HE) (DnaB-RFs) (30 DnaB hexamers/substrate). The DnaB RFs can efficiently utilize the DNA substrate (60% in 5 min at 30C), and the forks move at a rapid rate (550-780 bp/sec at 30C). The DnaB-RFs have an average maximal processivity of 40,000 nt, and addition of either SSB or primase increase the processivity (150,000 nt + SSB, 70,000-140,000 nt + primase). However, SSB and primase do not affect the rate of fork movement or the amount of substrate utilized in the assay. The [lambda] SS proteins are effective at transferring DnaB onto the DNA substrate (8 DnaB hexamers/substrate). The [lambda] SS proteins do not change the rate of RF movement or the amount of substrate utilized. However, the amount of synthesis measured in the assay is [approximately]2-fold higher in the presence of the [lambda] SS proteins. Therefore, the [lambda] SS proteins increase the processivity of DnaB at the RF (100,000 nt). The [lambda] SS proteins do not appear to play a role in elongation because the processivity of the RF in the presence of SSB and primase is equivalent to the processivity of the [lambda] SS-RFs. [lambda] P protein blocks DnaB helicase activity if added to the RF assay prior to initiation or during elongation. DnaB helicase is more resistant to P inhibition, if the helicase is allowed to bind to the substrate prior to addition of [lambda] P or if primase and rNTPs are included in the assay. These results suggest that the conformation of the RF complex (DNA or nucleoprotein structure) blocks the attack of P on DnaB helicase. The heat shock proteins may play an auxiliary role in mediating the effects of [lambda] P if the concentration of P protein in the cells are high.

Stephens, K.M.

1991-01-01T23:59:59.000Z

374

Automating DNA processing  

E-Print Network (OSTI)

Technology is currently available to identify the genetic codes responsible for physical traits and genetic diseases in both plants and animals. Regardless of whether the final goal is medical diagnosis or breeder selection, extensive time and resources must be spent in laboratory research to determine the genetic structure of the relevant organisms. DNA processing is riddled with time intensive laboratory techniques that must be improved or replaced if genotyping large numbers of samples is to be accomplished. This thesis identifies and explains modules in DNA processing and how they can be improved by automation. modules associated with genome mapping are the focus of most of the discussion. A functional biochemistry background is provided so that researchers in automation can be efficiently assimilated to future biochen-fistry/automation projects. The needs of biochemistry researchers at Texas A&M University are specifically addressed. Herein, DNA processing has been defined as a series of discrete sub-processes or process modules in order to aid scheduling of future automation projects. Target process modules (sub-processes with a high probability of automation success) have been identified. In addition, possible automation solutions have been proposed for each target module along with a characterization of fundamental design parameters. Concluding this text is a discussion of procedures in genome mapping that have not been sufficiently automated. The initial focus of this thesis is on short term solutions. However, attention is given to more conceptual solutions accompanied by the biochemistry background necessary to begin developing them. Though systems are proposed to improve the efficiency of many processes, no implementation has been attempted. Design specifications are based on observation of current laboratory techniques and the variance that is typically allowed in relevant process parameters in TAMU laboratories.

Wienen, Michael Jan

1994-01-01T23:59:59.000Z

375

DNA-based asymmetric catalysis.  

E-Print Network (OSTI)

??The goal of the research described in this thesis was to develop the general concept and methodology of DNA-based asymmetric catalysis, with the aim of (more)

Boersma, Arnold Jacob

2009-01-01T23:59:59.000Z

376

General Lightning Information  

NLE Websites -- All DOE Office Websites (Extended Search)

transfers a charge of five coulombs and 500 MJ, or enough energy to power a 100 watt light bulb for just under two months. The voltage depends on the length of the bolt, with the...

377

Sequence independent amplification of DNA  

DOE Patents (OSTI)

The present invention is a rapid sequence-independent amplification procedure (SIA). Even minute amounts of DNA from various sources can be amplified independent of any sequence requirements of the DNA or any a priori knowledge of any sequence characteristics of the DNA to be amplified. This method allows, for example, the sequence independent amplification of microdissected chromosomal material and the reliable construction of high quality fluorescent in situ hybridization (FISH) probes from YACs or from other sources. These probes can be used to localize YACs on metaphase chromosomes but also--with high efficiency--in interphase nuclei. 25 figs.

Bohlander, S.K.

1998-03-24T23:59:59.000Z

378

Sequence independent amplification of DNA  

DOE Patents (OSTI)

The present invention is a rapid sequence-independent amplification procedure (SIA). Even minute amounts of DNA from various sources can be amplified independent of any sequence requirements of the DNA or any a priori knowledge of any sequence characteristics of the DNA to be amplified. This method allows, for example the sequence independent amplification of microdissected chromosomal material and the reliable construction of high quality fluorescent in situ hybridization (FISH) probes from YACs or from other sources. These probes can be used to localize YACs on metaphase chromosomes but also--with high efficiency--in interphase nuclei.

Bohlander, Stefan K. (Chicago, IL)

1998-01-01T23:59:59.000Z

379

Physical Interactions between Mcm10, DNA, and DNA Polymerase [alpha  

SciTech Connect

Mcm10 is an essential eukaryotic protein required for the initiation and elongation phases of chromosomal replication. Specifically, Mcm10 is required for the association of several replication proteins, including DNA polymerase {alpha} (pol {alpha}), with chromatin. We showed previously that the internal (ID) and C-terminal (CTD) domains of Mcm10 physically interact with both single-stranded (ss) DNA and the catalytic p180 subunit of pol {alpha}. However, the mechanism by which Mcm10 interacts with pol {alpha} on and off DNA is unclear. As a first step toward understanding the structural details for these critical intermolecular interactions, x-ray crystallography and NMR spectroscopy were used to map the binary interfaces between Mcm10-ID, ssDNA, and p180. The crystal structure of an Mcm10-ID {center_dot} ssDNA complex confirmed and extended our previous evidence that ssDNA binds within the oligonucleotide/oligosaccharide binding-fold cleft of Mcm10-ID. We show using NMR chemical shift perturbation and fluorescence spectroscopy that p180 also binds to the OB-fold and that ssDNA and p180 compete for binding to this motif. In addition, we map a minimal Mcm10 binding site on p180 to a small region within the p180 N-terminal domain (residues 286-310). These findings, together with data for DNA and p180 binding to an Mcm10 construct that contains both the ID and CTD, provide the first mechanistic insight into how Mcm10 might use a handoff mechanism to load and stabilize pol {alpha} within the replication fork.

Warren, Eric M.; Huang, Hao; Fanning, Ellen; Chazin, Walter J.; Eichman, Brandt F.; (Vanderbilt)

2009-10-21T23:59:59.000Z

380

When DNA Needs to Stand Up and Be Counted  

NLE Websites -- All DOE Office Websites (Extended Search)

When DNA Needs to Stand Up and Be Counted Print DNA microarrays are small metal, glass, or silicon chips covered with patterns of short single-stranded DNA (ssDNA). These "DNA...

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


381

Solderability study of 63Sn-37Pb on zinc-plated and cadmium-plated stainless steel for the MC4636 lightning arrestor connector.  

Science Conference Proceedings (OSTI)

Cadmium plating on metal surfaces is commonly used for corrosion protection and to achieve good solderability on the 304L stainless steel shell of the MC4636 lightning arrestor connector (LAC) for the W76-1 system. This study examined the use of zinc as a potential substitute for the cadmium protective surface finish. Tests were performed with an R and RMA flux and test temperatures of 230 C, 245 C, and 260 C. Contact angle, {theta}{sub c}, served as the generalized solderability metric. The wetting rate and wetting time parameters were also collected. The solderability ({theta}{sub c}) of the Erie Plating Cd/Ni coatings was better than that of similar Amphenol coatings. Although the {theta}{sub c} data indicated that both Cd/Ni platings would provide adequate solderability, the wetting rate and wetting time data showed the Amphenol coatings to have better performance. The Zn/Ni coatings exhibited non-wetting under all flux and temperature conditions. Based on the results of these tests, it has been demonstrated that zinc plating is not a viable alternate to cadmium plating for the LAC connectors.

Lopez, Edwin Paul; Vianco, Paul Thomas; Rejent, Jerome Andrew; Martin, Joseph J.

2004-06-01T23:59:59.000Z

382

Cellular responses to environmental DNA damage  

Science Conference Proceedings (OSTI)

This volume contains the proceedings of the conference entitled Cellular Responses to Environmental DNA Damage held in Banff,Alberta December 1--6, 1991. The conference addresses various aspects of DNA repair in sessions titled DNA repair; Basic Mechanisms; Lesions; Systems; Inducible Responses; Mutagenesis; Human Population Response Heterogeneity; Intragenomic DNA Repair Heterogeneity; DNA Repair Gene Cloning; Aging; Human Genetic Disease; and Carcinogenesis. Individual papers are represented as abstracts of about one page in length.

Not Available

1994-08-01T23:59:59.000Z

383

Regulation of DNA damage tolerance : studies of the translesion synthesis DNA ploymerase eta in Saccharomyces cerevisiae  

E-Print Network (OSTI)

All organisms must control the effects of DNA damage to protect the integrity of their genomes. In addition to DNA repair, this requires DNA damage tolerance pathways, which allow the continuation of essential processes ...

Woodruff, Rachel Van Etten

2008-01-01T23:59:59.000Z

384

Characterization of nanoparticle-DNA conjugate and control of DNA conformation on particle surface  

E-Print Network (OSTI)

Nano-science has exploited the hybridization and de-hybridization phenomena of DNA which are one of its fundamental functions. In particular, conjugates of gold nanoparticles and DNA (Au NP-DNA) have been extensively ...

Park, Sunho, 1976-

2009-01-01T23:59:59.000Z

385

Preparation Of Dna-Containing Extract For Pcr Amplification  

NLE Websites -- All DOE Office Websites (Extended Search)

Preparation Of Dna-Containing Extract For Pcr Amplification Preparation Of Dna-Containing Extract For Pcr Amplification The method may provide a DNA-containing extract sufficiently...

386

Silencing and recombination in yeast ribosomal DNA  

E-Print Network (OSTI)

A bioinformatic and laboratory investigation into S. cerevisiae's system for the maintenance and homogenization of rDNA. Eliminating mutations and heterogeneity in rDNA repeats is necessary evolutionarily, but harmful to ...

O'Kelly, Michael J. T

2008-01-01T23:59:59.000Z

387

Low-cost, Rapid DNA Sequencing Technique  

Sequencing DNA is crucial for future breakthroughs in biological and biomedical research. Until now, ... The nucleic acid strand transport

388

Amplification of chromosomal DNA in situ  

DOE Patents (OSTI)

Amplification of chromosomal DNA in situ to increase the amount of DNA associated with a chromosome or chromosome region is described. The amplification of chromosomal DNA in situ provides for the synthesis of Fluorescence in situ Hybridization (FISH) painting probes from single dissected chromosome fragments, the production of cDNA libraries from low copy mRNAs and improved in Comparative Genomic Hybridization (CGH) procedures.

Christian, Allen T. (Tracy, CA); Coleman, Matthew A. (Livermore, CA); Tucker, James D. (Livermore, CA)

2002-01-01T23:59:59.000Z

389

Topics and Techniques in Forensic DNA Analysis  

Science Conference Proceedings (OSTI)

... Topics and Techniques for Forensic DNA Analysis NYC OCME Dept of Forensic Biology ... NIST Human Identity Project Leader (1999-present) ...

2012-04-17T23:59:59.000Z

390

Quantum Dot Fluorescence Lifetime Engineering with DNA ...  

Science Conference Proceedings (OSTI)

Quantum Dot Fluorescence Lifetime Engineering with DNA Origami ... such as metal nanoparticles and semiconductor quantum dots is challenging ...

391

Data hiding methods based upon DNA sequences  

Science Conference Proceedings (OSTI)

In this paper, three data hiding methods are proposed, based upon properties of DNA sequences. It is highlighted that DNA sequences possess some interesting properties which can be utilized to hide data. These three methods are: the Insertion Method, ... Keywords: Complementary pair, DNA, Data hiding, Data recovery

H. J. Shiu; K. L. Ng; J. F. Fang; R. C. T. Lee; C. H. Huang

2010-06-01T23:59:59.000Z

392

Magnetic tweezers to study DNA motors  

E-Print Network (OSTI)

Magnetic tweezers to study DNA motors Maria Mañosas Ritort lab UB Barcelona Croquette-Bensimon lab ENS France #12;· Introduction to MT (magnetic tweezers) · Applications: 1. Tracking DNA motors: (i) Helicases (ii) Annealing motor 2. Studying a multiprotein system: DNA replication Outline #12;· Atomic force

Ritort, Felix

393

Probe and method for DNA detection  

SciTech Connect

A hybridization probe containing two linear strands of DNA lights up upon hybridization to a target DNA using silver nanoclusters that have been templated onto one of the DNA strands. Hybridization induces proximity between the nanoclusters on one strand and an overhang on the other strand, which results in enhanced fluorescence emission from the nanoclusters.

Yeh, Hsin-Chih; Werner, James Henry; Sharma, Jaswinder Kumar; Martinez, Jennifer Suzanne

2013-07-02T23:59:59.000Z

394

Binary electrokinetic separation of target DNA from background DNA primers.  

Science Conference Proceedings (OSTI)

This report contains the summary of LDRD project 91312, titled ''Binary Electrokinetic Separation of Target DNA from Background DNA Primers''. This work is the first product of a collaboration with Columbia University and the Northeast BioDefense Center of Excellence. In conjunction with Ian Lipkin's lab, we are developing a technique to reduce false positive events, due to the detection of unhybridized reporter molecules, in a sensitive and multiplexed detection scheme for nucleic acids developed by the Lipkin lab. This is the most significant problem in the operation of their capability. As they are developing the tools for rapidly detecting the entire panel of hemorrhagic fevers this technology will immediately serve an important national need. The goal of this work was to attempt to separate nucleic acid from a preprocessed sample. We demonstrated the preconcentration of kilobase-pair length double-stranded DNA targets, and observed little preconcentration of 60 base-pair length single-stranded DNA probes. These objectives were accomplished in microdevice formats that are compatible with larger detection systems for sample pre-processing. Combined with Columbia's expertise, this technology would enable a unique, fast, and potentially compact method for detecting/identifying genetically-modified organisms and multiplexed rapid nucleic acid identification. Another competing approach is the DARPA funded IRIS Pharmaceutical TIGER platform which requires many hours for operation, and an 800k$ piece of equipment that fills a room. The Columbia/SNL system could provide a result in 30 minutes, at the cost of a few thousand dollars for the platform, and would be the size of a shoebox or smaller.

James, Conrad D.; Derzon, Mark Steven

2005-10-01T23:59:59.000Z

395

Microfluidic DNA sample preparation method and device  

DOE Patents (OSTI)

Manipulation of DNA molecules in solution has become an essential aspect of genetic analyses used for biomedical assays, the identification of hazardous bacterial agents, and in decoding the human genome. Currently, most of the steps involved in preparing a DNA sample for analysis are performed manually and are time, labor, and equipment intensive. These steps include extraction of the DNA from spores or cells, separation of the DNA from other particles and molecules in the solution (e.g. dust, smoke, cell/spore debris, and proteins), and separation of the DNA itself into strands of specific lengths. Dielectrophoresis (DEP), a phenomenon whereby polarizable particles move in response to a gradient in electric field, can be used to manipulate and separate DNA in an automated fashion, considerably reducing the time and expense involved in DNA analyses, as well as allowing for the miniaturization of DNA analysis instruments. These applications include direct transport of DNA, trapping of DNA to allow for its separation from other particles or molecules in the solution, and the separation of DNA into strands of varying lengths.

Krulevitch, Peter A. (Pleasanton, CA); Miles, Robin R. (Danville, CA); Wang, Xiao-Bo (San Diego, CA); Mariella, Raymond P. (Danville, CA); Gascoyne, Peter R. C. (Bellaire, TX); Balch, Joseph W. (Livermore, CA)

2002-01-01T23:59:59.000Z

396

Human Genome Research: Decoding DNA  

NLE Websites -- All DOE Office Websites (Extended Search)

Human Genome Research: Decoding DNA Human Genome Research: Decoding DNA Resources with Additional Information Charles DeLisi As head of DOE's Office of Health and Environmental Research, Charles DeLisi played a pivotal role in proposing and initiating the Human Genome Program in 1986. The U.S. Department of Energy (DOE) has historically been active in supporting human genome research. On September 10, 2003, Secretary of Energy Spencer Abraham presented the Secretary's Gold Award to Aristides Patrinos and Francis Collins for their leadership of the government's Human Genome Project. At DOE's Office of Science, Dr. Patrinos is the Associate Director for Biological and Environmental Research. He has been a researcher at the department's Oak Ridge National Laboratory and Brookhaven National Laboratory.

397

Fleet DNA Project (Fact Sheet)  

SciTech Connect

The Fleet DNA Project - designed by the U.S. Department of Energy's National Renewable Energy Laboratory (NREL) in partnership with Oak Ridge National Laboratory - aims to accelerate the evolution of advanced vehicle development and support the strategic deployment of market-ready technologies that reduce costs, fuel consumption, and emissions. At the heart of the Fleet DNA Project is a clearinghouse of medium- and heavy-duty commercial fleet transportation data for optimizing the design of advanced vehicle technologies or for selecting a given technology to invest in. An easy-to-access online database will help vehicle manufacturers and fleets understand the broad operational range for many of today's commercial vehicle vocations.

Not Available

2012-10-01T23:59:59.000Z

398

Channel plate for DNA sequencing  

DOE Patents (OSTI)

This invention is a channel plate that facilitates data compaction in DNA sequencing. The channel plate has a length, a width and a thickness, and further has a plurality of channels that are parallel. Each channel has a depth partially through the thickness of the channel plate. Additionally an interface edge permits electrical communication across an interface through a buffer to a deposition membrane surface.

Douthart, Richard J. (Richland, WA); Crowell, Shannon L. (Eltopia, WA)

1998-01-01T23:59:59.000Z

399

Channel plate for DNA sequencing  

DOE Patents (OSTI)

This invention is a channel plate that facilitates data compaction in DNA sequencing. The channel plate has a length, a width and a thickness, and further has a plurality of channels that are parallel. Each channel has a depth partially through the thickness of the channel plate. Additionally an interface edge permits electrical communication across an interface through a buffer to a deposition membrane surface. 15 figs.

Douthart, R.J.; Crowell, S.L.

1998-01-13T23:59:59.000Z

400

A four-year summertime microburst climatology and relationship between microbursts and cloud-to ground lightning flash rate for the NASA Kennedy Space Center, Florida: 1995-1998  

E-Print Network (OSTI)

In order to ameliorate the forecasting of microbursts, the first summertime microburst climatology in the United States was produced. This climatology was based on a four year wind data base during the summer months (May - September) of 1995-1998 for 50 mesons wind towers that encompasses the KSC. An investigation into the microburst characteristics of frequency, diurnal variation, spatial variation, speed frequency distribution, and the wind direction was accomplished. Finally, an examination into the relationship between microbursts and lightning was conducted. A total of 282 microbursts were observed during this four-year period. There were 114 microburst days with 59 of these days having more than one microburst. The most prominent months of microbursts are June, July, and August with July being the most dominant. Several important characteristics of wind speed and direction were found. The median wind speed was 34 knots. The majority of microburst wind speeds fell within 25 and 44 knots. As the wind increases above 43 knots, the frequency decreases exponentially, reaching virtually zero at 65 knots. The predominant wind direction of the microbursts is from southeast through the west-northwest with a maximum from the southwest. The spatial variation of the microbursts revealed an interesting pattern. Some areas received a substantial amount of microbursts, while others received few, if any. It is hypothesized that this difference is due to the sea breeze interacting with the abundant over breezes causing the formation of convection over the same areas on a daily basis. The diurnal variation also uncovered a revealing pattern. The most favorable time for microbursts is between 1600 UTC (12 P.M. EDT) and 2200 UTC (6 P.M. EDT) with the peak occurring between 2000 UTC (4 P.M. EDT) and 2200 UTC (6 P.M. EDT). Directly after this peak the number of microbursts drops drastically. The investigation into the relationship between lightning and microbursts revealed that in most cases there was an evident increase in the CG flash rate up to 25 minutes prior to the microburst. Moreover, a clear peak omen occurred 5-10 minutes before the microburst. Thus, CG lightning may also improve microburst forecasting.

Sanger, Neil Thomas

1999-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


401

DNA Sequencing Using capillary Electrophoresis  

Science Conference Proceedings (OSTI)

The overall goal of this program was to develop capillary electrophoresis as the tool to be used to sequence for the first time the Human Genome. Our program was part of the Human Genome Project. In this work, we were highly successful and the replaceable polymer we developed, linear polyacrylamide, was used by the DOE sequencing lab in California to sequence a significant portion of the human genome using the MegaBase multiple capillary array electrophoresis instrument. In this final report, we summarize our efforts and success. We began our work by separating by capillary electrophoresis double strand oligonucleotides using cross-linked polyacrylamide gels in fused silica capillaries. This work showed the potential of the methodology. However, preparation of such cross-linked gel capillaries was difficult with poor reproducibility, and even more important, the columns were not very stable. We improved stability by using non-cross linked linear polyacrylamide. Here, the entangled linear chains could move when osmotic pressure (e.g. sample injection) was imposed on the polymer matrix. This relaxation of the polymer dissipated the stress in the column. Our next advance was to use significantly lower concentrations of the linear polyacrylamide that the polymer could be automatically blown out after each run and replaced with fresh linear polymer solution. In this way, a new column was available for each analytical run. Finally, while testing many linear polymers, we selected linear polyacrylamide as the best matrix as it was the most hydrophilic polymer available. Under our DOE program, we demonstrated initially the success of the linear polyacrylamide to separate double strand DNA. We note that the method is used even today to assay purity of double stranded DNA fragments. Our focus, of course, was on the separation of single stranded DNA for sequencing purposes. In one paper, we demonstrated the success of our approach in sequencing up to 500 bases. Other application papers of sequencing up to this level were also published in the mid 1990's. A major interest of the sequencing community has always been read length. The longer the sequence read per run the more efficient the process as well as the ability to read repeat sequences. We therefore devoted a great deal of time to studying the factors influencing read length in capillary electrophoresis, including polymer type and molecule weight, capillary column temperature, applied electric field, etc. In our initial optimization, we were able to demonstrate, for the first time, the sequencing of over 1000 bases with 90% accuracy. The run required 80 minutes for separation. Sequencing of 1000 bases per column was next demonstrated on a multiple capillary instrument. Our studies revealed that linear polyacrylamide produced the longest read lengths because the hydrophilic single strand DNA had minimal interaction with the very hydrophilic linear polyacrylamide. Any interaction of the DNA with the polymer would lead to broader peaks and lower read length. Another important parameter was the molecular weight of the linear chains. High molecular weight (> 1 MDA) was important to allow the long single strand DNA to reptate through the entangled polymer matrix. In an important paper, we showed an inverse emulsion method to prepare reproducibility linear polyacrylamide polymer with an average MWT of 9MDa. This approach was used in the polymer for sequencing the human genome. Another critical factor in the successful use of capillary electrophoresis for sequencing was the sample preparation method. In the Sanger sequencing reaction, high concentration of salts and dideoxynucleotide remained. Since the sample was introduced to the capillary column by electrokinetic injection, these salt ions would be favorably injected into the column over the sequencing fragments, thus reducing the signal for longer fragments and hence reading read length. In two papers, we examined the role of individual components from the sequencing reaction and then developed a protocol to reduce the deleterio

Dr. Barry Karger

2011-05-09T23:59:59.000Z

402

Enhancing the DNA Patent Database  

Science Conference Proceedings (OSTI)

Final Report on Award No. DE-FG0201ER63171 Principal Investigator: LeRoy B. Walters February 18, 2008 This project successfully completed its goal of surveying and reporting on the DNA patenting and licensing policies at 30 major U.S. academic institutions. The report of survey results was published in the January 2006 issue of Nature Biotechnology under the title The Licensing of DNA Patents by US Academic Institutions: An Empirical Survey. Lori Pressman was the lead author on this feature article. A PDF reprint of the article will be submitted to our Program Officer under separate cover. The project team has continued to update the DNA Patent Database on a weekly basis since the conclusion of the project. The database can be accessed at dnapatents.georgetown.edu. This database provides a valuable research tool for academic researchers, policymakers, and citizens. A report entitled Reaping the Benefits of Genomic and Proteomic Research: Intellectual Property Rights, Innovation, and Public Health was published in 2006 by the Committee on Intellectual Property Rights in Genomic and Protein Research and Innovation, Board on Science, Technology, and Economic Policy at the National Academies. The report was edited by Stephen A. Merrill and Anne-Marie Mazza. This report employed and then adapted the methodology developed by our research project and quoted our findings at several points. (The full report can be viewed online at the following URL: http://www.nap.edu/openbook.php?record_id=11487&page=R1). My colleagues and I are grateful for the research support of the ELSI program at the U.S. Department of Energy.

Walters, LeRoy B.

2008-02-18T23:59:59.000Z

403

DNA-guided nanoparticle assemblies  

DOE Patents (OSTI)

In some embodiments, DNA-capped nanoparticles are used to define a degree of crystalline order in assemblies thereof. In some embodiments, thermodynamically reversible and stable body-centered cubic (bcc) structures, with particles occupying <.about.10% of the unit cell, are formed. Designs and pathways amenable to the crystallization of particle assemblies are identified. In some embodiments, a plasmonic crystal is provided. In some aspects, a method for controlling the properties of particle assemblages is provided. In some embodiments a catalyst is formed from nanoparticles linked by nucleic acid sequences and forming an open crystal structure with catalytically active agents attached to the crystal on its surface or in interstices.

Gang, Oleg; Nykypanchuk, Dmytro; Maye, Mathew; van der Lelie, Daniel

2013-07-16T23:59:59.000Z

404

Immunoglobulin motif DNA recognition and  

E-Print Network (OSTI)

binding domains of NF-kB, NFAT1, p53 and the STAT proteins. NMR spectroscopy of a 43.6 kD RD­b­DNA ternary for NF-kB3-4, the nuclear factor of acti- vated T-cells NFAT1 (refs 5,6), STAT1 (ref. 7) and STAT3b8 72 and Ser 73. In con- trast, the N-terminus of NF-kB3-4 and NFAT1 (refs 5, 6) loops around

Sali, Andrej

405

DNA Directed Assembly Probe for Detecting DNA-Protein Interaction in Microarray Format  

E-Print Network (OSTI)

Quantifying DNA-protein interaction using DNA microarrays are gaining increasing attention due to their ability to profile specificity of interactions in a high-throughput manner. This paper describes a new approach that ...

Ng, Jin Kiat

406

DNA sequencing using fluorescence background electroblotting membrane  

DOE Patents (OSTI)

A method for the multiplex sequencing on DNA is disclosed which comprises the electroblotting or specific base terminated DNA fragments, which have been resolved by gel electrophoresis, onto the surface of a neutral non-aromatic polymeric microporous membrane exhibiting low background fluorescence which has been surface modified to contain amino groups. Polypropylene membranes are preferably and the introduction of amino groups is accomplished by subjecting the membrane to radio or microwave frequency plasma discharge in the presence of an aminating agent, preferably ammonia. The membrane, containing physically adsorbed DNA fragments on its surface after the electroblotting, is then treated with crosslinking means such as UV radiation or a glutaraldehyde spray to chemically bind the DNA fragments to the membrane through said smino groups contained on the surface thereof. The DNA fragments chemically bound to the membrane are subjected to hybridization probing with a tagged probe specific to the sequence of the DNA fragments. The tagging may be by either fluorophores or radioisotopes. The tagged probes hybridized to said target DNA fragments are detected and read by laser induced fluorescence detection or autoradiograms. The use of aminated low fluorescent background membranes allows the use of fluorescent detection and reading even when the available amount of DNA to be sequenced is small. The DNA bound to the membrances may be reprobed numerous times.

Caldwell, Karin D. (Salt Lake City, UT); Chu, Tun-Jen (Salt Lake City, UT); Pitt, William G. (Orem, UT)

1992-01-01T23:59:59.000Z

407

DNA sequencing using fluorescence background electroblotting membrane  

DOE Patents (OSTI)

A method for the multiplex sequencing on DNA is disclosed which comprises the electroblotting or specific base terminated DNA fragments, which have been resolved by gel electrophoresis, onto the surface of a neutral non-aromatic polymeric microporous membrane exhibiting low background fluorescence which has been surface modified to contain amino groups. Polypropylene membranes are preferably and the introduction of amino groups is accomplished by subjecting the membrane to radio or microwave frequency plasma discharge in the presence of an aminating agent, preferably ammonia. The membrane, containing physically adsorbed DNA fragments on its surface after the electroblotting, is then treated with crosslinking means such as UV radiation or a glutaraldehyde spray to chemically bind the DNA fragments to the membrane through amino groups contained on the surface. The DNA fragments chemically bound to the membrane are subjected to hybridization probing with a tagged probe specific to the sequence of the DNA fragments. The tagging may be by either fluorophores or radioisotopes. The tagged probes hybridized to the target DNA fragments are detected and read by laser induced fluorescence detection or autoradiograms. The use of aminated low fluorescent background membranes allows the use of fluorescent detection and reading even when the available amount of DNA to be sequenced is small. The DNA bound to the membranes may be reprobed numerous times. No Drawings

Caldwell, K.D.; Chu, T.J.; Pitt, W.G.

1992-05-12T23:59:59.000Z

408

Precision Biochemistry Tracks DNA Damage in Fish  

Science Conference Proceedings (OSTI)

... Like coal-mine canaries, fish DNA can serve as a measure of the biological impact of water and sediment pollutionor pollution clean-up. ...

2012-10-17T23:59:59.000Z

409

Topics in Forensic DNA Analysis & Interpretation  

Science Conference Proceedings (OSTI)

... Experience University of Virginia/FBI Laboratory (1992-1995) Work ... Most forensic DNA laboratories follow PCR ... for the polymer) If a lab is not ...

2011-03-24T23:59:59.000Z

410

Available Technologies: Highly Selective, Highly Efficient DNA ...  

... applications where extraction of minute amounts of DNA plays a critical role, such as in basic and applied molecular biology research, bioforensics, ...

411

Overview of DNA Programs at NIST  

Science Conference Proceedings (OSTI)

... during the PCR amplification process This is highly affected by DNA quantity and quality ... PCR inhibitors present in the sample may reduce PCR ...

2013-04-10T23:59:59.000Z

412

Bis[(1S)-1 4-azanediyl-1-(9-deazaadenin-9-yl)-1 4-dideoxy-5-methylsulfanyl-D-ribitol] tetrakis(hydrochloride) monohydrate: structure DFT energy and ligand docking results of a potent methylthioadenosine phosphorylase inhibitor found in different  

SciTech Connect

The title compound, abbreviated as 5'ThiomethylImmA, is a potent inhibitor of methylthioadenosine phosphorylase [Singh et al. (2004). Biochemistry, 43, 9-18]. The synchrotron study reported here shows that the hydrochloride salt crystallizes with two independent, nearly superimposable, dications as a monohydrate with formula 2C{sub 12}H{sub 19}N{sub 5}O{sub 2}S{sup 2+}{center_dot}4Cl{sup -}{center_dot}H{sub 2}O. Hydrogen bonding utilizing the H atoms of the dication is found to favor certain molecular conformations in the salt, which are significantly different from those found as bound in the enzyme. Ligand docking studies starting from either of these dications or related neutral structures successfully place the conformationally revised structures in the enzyme active site but only under particular hydrogen-bonding and molecular flexibility criteria. Density functional theory calculations verify the energy similarity of the indendent cations and confirm the significant energy cost of the required conformation change to the enzyme bound form. The results suggest the using crystallographically determined free ligand coordinates as starting parameters for modelling may have serious limitations.

G Gainsford; G Evans; K Johnston; M Seth

2011-12-31T23:59:59.000Z

413

DOI-BLM-NM-L000-2012-0046-CX | Open Energy Information  

Open Energy Info (EERE)

6-CX 6-CX Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home NEPA Document Collection for: DOI-BLM-NM-L000-2012-0046-CX CX at Lightning Dock Geothermal Area for Geothermal/Exploration {{{NEPA_Name}}} General NEPA Document Info Energy Sector Geothermal energy Environmental Analysis Type CX Applicant Lightning Dock Geothermal Inc Geothermal Area Lightning Dock Geothermal Area Project Location New Mexico Project Phase Geothermal/Exploration Techniques Time Frame (days) Application Time 16 Participating Agencies Lead Agency BLM Funding Agency none provided Managing District Office BLM Las Cruces District Office Managing Field Office none provided Funding Agencies none provided Surface Manager none provided Mineral Manager BLM Selected Dates Application Date 1/4/2012

414

Property:NEPA DNA Worksheet | Open Energy Information  

Open Energy Info (EERE)

DNA Worksheet DNA Worksheet Jump to: navigation, search Property Name NEPA DNA Worksheet Property Type Page Description DNA Worksheet files for NEPA Docs. This is a property of type Page. It links to pages that use the form NEPA_Doc. Pages using the property "NEPA DNA Worksheet" Showing 19 pages using this property. D DOI-BLM-NV-C010-2011-0517-DNA + DOI-BLM-NV-C010-2011-0517-DNA.pdf + DOI-BLM-NV-C010-2012--044-DNA + DOI-BLM-NV-C010-2012-0044-DNA.pdf + DOI-BLM-NV-C010-2012-0005-DNA + DOI-BLM-NV-C010-2012-0005-DNA.pdf + DOI-BLM-NV-C010-2012-0016-DNA + DOI-BLM-NV-C010-2012-0016-DNA.pdf + DOI-BLM-NV-C010-2012-0019-DNA + DOI-BLM-NV-C010-2012-0019-DNA.pdf + DOI-BLM-NV-C010-2012-0020-DNA + DOI-BLM-NV-C010-2012-0020-DNA.pdf + DOI-BLM-NV-C010-2012-0028-DNA + DOI-BLM-NV-C010-2012-0028-DNA.pdf +

415

Recombinant DNA encoding a desulfurization biocatalyst  

DOE Patents (OSTI)

This invention relates to a recombinant DNA molecule containing a gene or genes which encode a biocatalyst capable of desulfurizing a fossil fuel which contains organic sulfur molecules. For example, the present invention encompasses a recombinant DNA molecule containing a gene or genes of a strain of Rhodococcus rhodochrous.

Rambosek, John (Seattle, WA); Piddington, Chris S. (Seattle, WA); Kovacevich, Brian R. (Seattle, WA); Young, Kevin D. (Grand Forks, ND); Denome, Sylvia A. (Thompson, ND)

1994-01-01T23:59:59.000Z

416

Recombinant DNA encoding a desulfurization biocatalyst  

DOE Patents (OSTI)

This invention relates to a recombinant DNA molecule containing a gene or genes which encode a biocatalyst capable of desulfurizing a fossil fuel which contains organic sulfur molecules. For example, the present invention encompasses a recombinant DNA molecule containing a gene or genes of a strain of Rhodococcus rhodochrous. 13 figs.

Rambosek, J.; Piddington, C.S.; Kovacevich, B.R.; Young, K.D.; Denome, S.A.

1994-10-18T23:59:59.000Z

417

The Initiation of Bacterial DNA Replication  

NLE Websites -- All DOE Office Websites (Extended Search)

The Initiation of Bacterial DNA The Initiation of Bacterial DNA Replication The Initiation of Bacterial DNA Replication Print Wednesday, 31 January 2007 00:00 For the first time, scientists have determined the structure of the initiator of bacterial DNA replication. It is already known that such replication is controlled by a protein known as DnaA, a member of the AAA+ superfamily of ATPases. What has now been discovered is that the core of the initiator is not the closed-ring structure expected for this system. Instead, DnaA forms an open right-handed helix. In addition, the architecture indicates that this AAA+ superhelix will wrap coils of the DNA around its exterior, causing the DNA double helix to deform as a first step in the separation and unwinding of its strands. Eukaryotic and archaeal initiators also have the structural elements that promote open-helix formation, indicating that a spiral, open-ring AAA+ assembly is a conserved element from a common evolutionary ancestor of Archaea, Bacteria, and Eukarya.

418

When DNA Needs to Stand Up and Be Counted  

NLE Websites -- All DOE Office Websites (Extended Search)

When DNA Needs to Stand Up and Be Counted When DNA Needs to Stand Up and Be Counted Print Wednesday, 31 May 2006 00:00 DNA microarrays are small metal, glass, or silicon chips...

419

Allosteric Modulation of DNA by Small Molecules  

NLE Websites -- All DOE Office Websites (Extended Search)

Allosteric Modulation of DNA by Small Allosteric Modulation of DNA by Small Molecules Signals originating at the cell surface are conveyed by a complex system of interconnected signaling pathways to the nucleus. They converge at transcription factors, which in turn regulate the transcription of sets of genes that result in the gene expression. Many human diseases are caused by dysregulated gene expression and the oversupply of transcription factors may be required for the growth and metastatic behavior of human cancers. Cell permeable small molecules that can be programmed to disrupt transcription factor-DNA interfaces could silence aberrant gene expression pathways. Pyrrole-imidazole polyamides are DNA minor groove binding small molecules that are programmable for a large repertoire of DNA motifs.

420

Storing data encoded DNA in living organisms  

DOE Patents (OSTI)

Current technologies allow the generation of artificial DNA molecules and/or the ability to alter the DNA sequences of existing DNA molecules. With a careful coding scheme and arrangement, it is possible to encode important information as an artificial DNA strand and store it in a living host safely and permanently. This inventive technology can be used to identify origins and protect R&D investments. It can also be used in environmental research to track generations of organisms and observe the ecological impact of pollutants. Today, there are microorganisms that can survive under extreme conditions. As well, it is advantageous to consider multicellular organisms as hosts for stored information. These living organisms can provide as memory housing and protection for stored data or information. The present invention provides well for data storage in a living organism wherein at least one DNA sequence is encoded to represent data and incorporated into a living organism.

Wong; Pak C. (Richland, WA), Wong; Kwong K. (Sugar Land, TX), Foote; Harlan P. (Richland, WA)

2006-06-06T23:59:59.000Z

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


421

Method for sequencing DNA base pairs  

DOE Patents (OSTI)

The base pairs of a DNA structure are sequenced with the use of a scanning tunneling microscope (STM). The DNA structure is scanned by the STM probe tip, and, as it is being scanned, the DNA structure is separately subjected to a sequence of infrared radiation from four different sources, each source being selected to preferentially excite one of the four different bases in the DNA structure. Each particular base being scanned is subjected to such sequence of infrared radiation from the four different sources as that particular base is being scanned. The DNA structure as a whole is separately imaged for each subjection thereof to radiation from one only of each source. 6 figures.

Sessler, A.M.; Dawson, J.

1993-12-14T23:59:59.000Z

422

Improved method for sequencing DNA base pairs  

DOE Patents (OSTI)

The base pairs of a DNA structure are sequenced with the use of a scanning tunneling microscope (STM). The DNA structure is scanned by the STM probe tip, and, as it is being scanned, the DNA structure is separately subjected to a sequence of infrared radiation from four different sources, each source being selected to preferentially excite one of the four different bases in the DNA structure. Each particular base being scanned is subjected to such sequence of infrared radiation from the four different sources as that particular base is being scanned. The DNA structure as a whole is separately imaged for each subjection thereof to radiation from one only of each source. 1 ref.

Sessler, A.M.; Dawson, J.

1990-12-31T23:59:59.000Z

423

Improved method for sequencing DNA base pairs  

DOE Patents (OSTI)

The base pairs of a DNA structure are sequenced with the use of a scanning tunneling microscope (STM). The DNA structure is scanned by the STM probe tip, and, as it is being scanned, the DNA structure is separately subjected to a sequence of infrared radiation from four different sources, each source being selected to preferentially excite one of the four different bases in the DNA structure. Each particular base being scanned is subjected to such sequence of infrared radiation from the four different sources as that particular base is being scanned. The DNA structure as a whole is separately imaged for each subjection thereof to radiation from one only of each source. 1 ref.

Sessler, A.M.; Dawson, J.

1990-01-01T23:59:59.000Z

424

Flow cytometric detection method for DNA samples  

Science Conference Proceedings (OSTI)

Disclosed herein are two methods for rapid multiplex analysis to determine the presence and identity of target DNA sequences within a DNA sample. Both methods use reporting DNA sequences, e.g., modified conventional Taqman.RTM. probes, to combine multiplex PCR amplification with microsphere-based hybridization using flow cytometry means of detection. Real-time PCR detection can also be incorporated. The first method uses a cyanine dye, such as, Cy3.TM., as the reporter linked to the 5' end of a reporting DNA sequence. The second method positions a reporter dye, e.g., FAM.TM. on the 3' end of the reporting DNA sequence and a quencher dye, e.g., TAMRA.TM., on the 5' end.

Nasarabadi,Shanavaz (Livermore, CA); Langlois, Richard G. (Livermore, CA); Venkateswaran, Kodumudi S. (Round Rock, TX)

2011-07-05T23:59:59.000Z

425

Flow cytometric detection method for DNA samples  

DOE Patents (OSTI)

Disclosed herein are two methods for rapid multiplex analysis to determine the presence and identity of target DNA sequences within a DNA sample. Both methods use reporting DNA sequences, e.g., modified conventional Taqman.RTM. probes, to combine multiplex PCR amplification with microsphere-based hybridization using flow cytometry means of detection. Real-time PCR detection can also be incorporated. The first method uses a cyanine dye, such as, Cy3.TM., as the reporter linked to the 5' end of a reporting DNA sequence. The second method positions a reporter dye, e.g., FAM, on the 3' end of the reporting DNA sequence and a quencher dye, e.g., TAMRA, on the 5' end.

Nasarabadi, Shanavaz (Livermore, CA); Langlois, Richard G. (Livermore, CA); Venkateswaran, Kodumudi S. (Livermore, CA)

2006-08-01T23:59:59.000Z

426

Flow cytometric detection method for DNA samples  

DOE Patents (OSTI)

Disclosed herein are two methods for rapid multiplex analysis to determine the presence and identity of target DNA sequences within a DNA sample. Both methods use reporting DNA sequences, e.g., modified conventional Taqman.RTM. probes, to combine multiplex PCR amplification with microsphere-based hybridization using flow cytometry means of detection. Real-time PCR detection can also be incorporated. The first method uses a cyanine dye, such as, Cy3.TM., as the reporter linked to the 5' end of a reporting DNA sequence. The second method positions a reporter dye, e.g., FAM.TM. on the 3' end of the reporting DNA sequence and a quencher dye, e.g., TAMRA.TM., on the 5' end.

Nasarabadi,Shanavaz (Livermore, CA); Langlois, Richard G. (Livermore, CA); Venkateswaran, Kodumudi S. (Round Rock, TX)

2011-07-05T23:59:59.000Z

427

Crystal Structure of the Chromodomain Helicase DNA-binding Protein 1 (Chd1) DNA-binding Domain in Complex with DNA  

Science Conference Proceedings (OSTI)

Chromatin remodelers are ATP-dependent machines that dynamically alter the chromatin packaging of eukaryotic genomes by assembling, sliding, and displacing nucleosomes. The Chd1 chromatin remodeler possesses a C-terminal DNA-binding domain that is required for efficient nucleosome sliding and believed to be essential for sensing the length of DNA flanking the nucleosome core. The structure of the Chd1 DNA-binding domain was recently shown to consist of a SANT and SLIDE domain, analogous to the DNA-binding domain of the ISWI family, yet the details of how Chd1 recognized DNA were not known. Here we present the crystal structure of the Saccharomyces cerevisiae Chd1 DNA-binding domain in complex with a DNA duplex. The bound DNA duplex is straight, consistent with the preference exhibited by the Chd1 DNA-binding domain for extranucleosomal DNA. Comparison of this structure with the recently solved ISW1a DNA-binding domain bound to DNA reveals that DNA lays across each protein at a distinct angle, yet contacts similar surfaces on the SANT and SLIDE domains. In contrast to the minor groove binding seen for Isw1 and predicted for Chd1, the SLIDE domain of the Chd1 DNA-binding domain contacts the DNA major groove. The majority of direct contacts with the phosphate backbone occur only on one DNA strand, suggesting that Chd1 may not strongly discriminate between major and minor grooves.

Sharma A.; Heroux A.; Jenkins K. R.; Bowman G. D.

2011-12-09T23:59:59.000Z

428

Ion Runaway in Lightning Discharges  

E-Print Network (OSTI)

Runaway ions can be produced in plasmas with large electric fields, where the accelerating electric force is augmented by the low mean ionic charge due to the imbalance between the number of electrons and ions. Here we ...

Landreman, Matt

429

Convective Cooling of Lightning Channels  

Science Conference Proceedings (OSTI)

We report experimental data which trace the time development of electric discharge channels in air and which demonstrate the turbulent cooling of such channels. These data provide qualitative confirmation of the model proposed and used by Hill, ...

J. M. Picone; J. P. Boris; J. R. Greig; M. Raleigh; R. F. Fernsler

1981-09-01T23:59:59.000Z

430

Electronic transport and localization in short and long DNA  

E-Print Network (OSTI)

The question of whether DNA conducts electric charges is intriguing to physicists and biologists alike. The suggestion that electron transfer/transport in DNA might be biologically important has triggered a series of experimental and theoretical investigations. Here, we review recent theoretical progress by concentrating on quantum-chemical, molecular dynamics-based approaches to short DNA strands and physics-motivated tight-binding transport studies of long or even complete DNA sequences. In both cases, we observe small, but significant differences between specific DNA sequences such as periodic repetitions and aperiodic sequences of AT bases, lambda-DNA, centromeric DNA, promoter sequences as well as random-ATGC DNA.

H. Wang; R. Marsh; J. P. Lewis; R. A. Roemer

2005-11-04T23:59:59.000Z

431

NREL: Fleet Test and Evaluation - Fleet DNA: Vehicle Drive Cycle...  

NLE Websites -- All DOE Office Websites (Extended Search)

Fleet DNA Project graphic depicting a trail of data emerging from trucks. Fleet DNA helps vehicle manufacturers and fleet managers understand the broad operational range for many...

432

Low-cost, Rapid DNA Sequencing Technique - Energy Innovation Portal  

Description Sequencing DNA is crucial for future breakthroughs in biological and biomedical research. ... DNA sequencing for medical applications has been restricted ...

433

Computational Challenges in Simulating Large DNA over Long Times  

Science Conference Proceedings (OSTI)

Simulating DNAs dynamics requires a sophisticated array of algorithms appropriate for DNAs impressive spectrum of spatial and temporal levels. The authors describe computational challenges

Tamar Schlick; Daniel A. Beard; Jing Huang; Daniel A. Strahs; Xiaoliang Qian

2000-01-01T23:59:59.000Z

434

The Initiation of Bacterial DNA Replication  

NLE Websites -- All DOE Office Websites (Extended Search)

The Initiation of Bacterial DNA Replication Print The Initiation of Bacterial DNA Replication Print For the first time, scientists have determined the structure of the initiator of bacterial DNA replication. It is already known that such replication is controlled by a protein known as DnaA, a member of the AAA+ superfamily of ATPases. What has now been discovered is that the core of the initiator is not the closed-ring structure expected for this system. Instead, DnaA forms an open right-handed helix. In addition, the architecture indicates that this AAA+ superhelix will wrap coils of the DNA around its exterior, causing the DNA double helix to deform as a first step in the separation and unwinding of its strands. Eukaryotic and archaeal initiators also have the structural elements that promote open-helix formation, indicating that a spiral, open-ring AAA+ assembly is a conserved element from a common evolutionary ancestor of Archaea, Bacteria, and Eukarya.

435

The Initiation of Bacterial DNA Replication  

NLE Websites -- All DOE Office Websites (Extended Search)

The Initiation of Bacterial DNA Replication Print The Initiation of Bacterial DNA Replication Print For the first time, scientists have determined the structure of the initiator of bacterial DNA replication. It is already known that such replication is controlled by a protein known as DnaA, a member of the AAA+ superfamily of ATPases. What has now been discovered is that the core of the initiator is not the closed-ring structure expected for this system. Instead, DnaA forms an open right-handed helix. In addition, the architecture indicates that this AAA+ superhelix will wrap coils of the DNA around its exterior, causing the DNA double helix to deform as a first step in the separation and unwinding of its strands. Eukaryotic and archaeal initiators also have the structural elements that promote open-helix formation, indicating that a spiral, open-ring AAA+ assembly is a conserved element from a common evolutionary ancestor of Archaea, Bacteria, and Eukarya.

436

Method of quantitating dsDNA  

DOE Patents (OSTI)

A method for quantitating dsDNA in an aqueous sample solution containing an unknown amount of dsDNA. A first aqueous test solution containing a known amount of a fluorescent dye-dsDNA complex and at least one fluorescence-attenutating contaminant is prepared. The fluorescence intensity of the test solution is measured. The first test solution is diluted by a known amount to provide a second test solution having a known concentration of dsDNA. The fluorescence intensity of the second test solution is measured. Additional diluted test solutions are similarly prepared until a sufficiently dilute test solution having a known amount of dsDNA is prepared that has a fluorescence intensity that is not attenuated upon further dilution. The value of the maximum absorbance of this solution between 200-900 nanometers (nm), referred to herein as the threshold absorbance, is measured. A sample solution having an unknown amount of dsDNA and an absorbance identical to that of the sufficiently dilute test solution at the same chosen wavelength is prepared. Dye is then added to the sample solution to form the fluorescent dye-dsDNA-complex, after which the fluorescence intensity of the sample solution is measured and the quantity of dsDNA in the sample solution is determined. Once the threshold absorbance of a sample solution obtained from a particular environment has been determined, any similarly prepared sample solution taken from a similar environment and having the same value for the threshold absorbance can be quantified for dsDNA by adding a large excess of dye to the sample solution and measuring its fluorescence intensity.

Stark, Peter C. (Los Alamos, NM); Kuske, Cheryl R. (Los Alamos, NM); Mullen, Kenneth I. (Los Alamos, NM)

2002-01-01T23:59:59.000Z

437

Everything a Trial Judge Needs to Know about DNA (in a ...  

Science Conference Proceedings (OSTI)

... evidence Paternity testing -- identifying father Missing persons investigations Military DNA dog tag Convicted felon DNA databases ...

2013-06-25T23:59:59.000Z

438

Torque determination on DNA with magnetic tweezers  

E-Print Network (OSTI)

We deduced the torque applied on a single stretched and twisted DNA by integrating with respect to force the change in the molecule's extension as it is coiled. While consistent with previous direct measurements of the torque at high forces (F>1 pN) this method, which is simple and does not require a sophisticated set-up, allows for lower force estimates. We used this approach to deduce the effective torsional modulus of DNA, which decreases with force and to estimate the buckling torque of DNA as a function of force in various salt conditions.

Francesco Mosconi; Jean-Franois Allemand; David Bensimon; Vincent Croquette

2008-09-03T23:59:59.000Z

439

Structural basis for DNA bending  

Science Conference Proceedings (OSTI)

The authors report proton NMR studies on DNA oligonucleotides that contain A tracts of lengths known to produce various degrees of bending. Spectra of duplexes in the series 5{prime}-(GGCA{sub n}CGG){center dot}(CCGT{sub n}GCC) (n = 3,4,5,7,9) reveal substantial structural changes within the A{sub n}{center dot}T{sub n} tract as its length is increased. Chemical-shift comparisons show that A tracts with fewer than about seven members do not contain regions of uniform structure. Throughout the series, there is a striking monotonic relationship between the location of an A{center dot}T pair in the A tract and the relative position of its ThyH3 resonance. The direction of this chemical-shift dispersion is opposite to that expected from consideration of ring-current effects alone. This model features a substantial negative base-pair tilt, which has been suggested previously as the source of A-tract bending. In contrast, the nuclear Overhauser effect distances are inconsistent with at least one known crystallographic A-tract structure which lacks appreciable base-pair tilt.

Nadeau, J.G.; Crothers, D.M. (Yale Univ., New Haven, CT (USA))

1989-04-01T23:59:59.000Z

440

Why DNA is a double helix  

NLE Websites -- All DOE Office Websites (Extended Search)

Guest14 Location: NA Country: NA Date: NA Question: Why is DNA in a double-helix shape? Replies: The why questions are always the worst. Why is anything the way it is? The...

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


441

Deoxyribose oxidation chemistry and endogenous DNA adducts  

E-Print Network (OSTI)

Endogenous and exogenous oxidants react with cellular macromolecules to generate a variety of electrophiles that react with DNA produce cytotoxic and mutagenic adducts. One source of such electrophiles is deoxyribose in ...

Zhou, Xinfeng

2006-01-01T23:59:59.000Z

442

Linear Thermodynamics of Rodlike DNA Filtration  

E-Print Network (OSTI)

Linear thermodynamics transportation theory is employed to study filtration of rodlike DNA molecules. Using the repeated nanoarray consisting of alternate deep and shallow regions, it is demonstrated that the complex ...

Li, Zirui

443

Multiple tag labeling method for DNA sequencing  

DOE Patents (OSTI)

A DNA sequencing method described which uses single lane or channel electrophoresis. Sequencing fragments are separated in said lane and detected using a laser-excited, confocal fluorescence scanner. Each set of DNA sequencing fragments is separated in the same lane and then distinguished using a binary coding scheme employing only two different fluorescent labels. Also described is a method of using radio-isotope labels.

Mathies, Richard A. (Contra Costa County, CA); Huang, Xiaohua C. (Mt. View, CA); Quesada, Mark A. (San Francisco, CA)

1995-01-01T23:59:59.000Z

444

Oligonucleotide and Long Polymeric DNA Encoding  

Science Conference Proceedings (OSTI)

This report summarizes the work done at Lawrence Livermore National Laboratory for the Oligonucleotide and Long Polymeric DNA Encoding project, part of the Microelectronic Bioprocesses Program at DARPA. The goal of the project was to develop a process by which long (circa 10,000 base-pair) synthetic DNA molecules could be synthesized in a timely and economic manner. During construction of the long molecule, errors in DNA sequence occur during hybridization and/or the subsequent enzymatic process. The work done on this project has resulted in a novel synthesis scheme that we call the parallel pyramid synthesis protocol, the development of a suit of computational tools to minimize and quantify errors in the synthesized DNA sequence, and experimental proof of this technique. The modeling consists of three interrelated modules: the bioinformatics code which determines the specifics of parallel pyramid synthesis for a given chain of long DNA, the thermodynamics code which tracks the products of DNA hybridization and polymerase extension during the later steps in the process, and the kinetics model which examines the temporal and spatial processes during one thermocycle. Most importantly, we conducted the first successful syntheses of a gene using small starting oligomers (tetramers). The synthesized sequence, 813 base pairs long, contained a 725 base pair gene, modified green fluorescent protein (mGFP), which has been shown to be a functional gene by cloning into cells and observing its green fluorescent product.

Miller, E; Mariella Jr., R P; Christian, A T; Gardner, S N; Williams, J M

2003-11-24T23:59:59.000Z

445

When DNA Needs to Stand Up and Be Counted  

NLE Websites -- All DOE Office Websites (Extended Search)

When DNA Needs to Stand Up and Be Counted Print When DNA Needs to Stand Up and Be Counted Print DNA microarrays are small metal, glass, or silicon chips covered with patterns of short single-stranded DNA (ssDNA). These "DNA chips" are revolutionizing biotechnology, allowing scientists to identify and count many DNA sequences simultaneously. They are the enabling technology for genomic-based medicine and are a critical component of advanced diagnostic systems for medical and homeland security applications. Like digital chips, DNA chips are parallel, accurate, fast, and small. These advantages, however, can only be realized if the fragile biomolecules survive the attachment process intact. Furthermore, biomolecules must be properly oriented to perform their biological function. In other words, the DNA literally must stand up to be counted. Understanding both the attachment and orientation of DNA on gold surfaces was the goal of recent experiments performed at ALS Beamline 8.0.1 by an international collaboration of scientists.

446

When DNA Needs to Stand Up and Be Counted  

NLE Websites -- All DOE Office Websites (Extended Search)

When DNA Needs to Stand Up and Be Counted Print When DNA Needs to Stand Up and Be Counted Print DNA microarrays are small metal, glass, or silicon chips covered with patterns of short single-stranded DNA (ssDNA). These "DNA chips" are revolutionizing biotechnology, allowing scientists to identify and count many DNA sequences simultaneously. They are the enabling technology for genomic-based medicine and are a critical component of advanced diagnostic systems for medical and homeland security applications. Like digital chips, DNA chips are parallel, accurate, fast, and small. These advantages, however, can only be realized if the fragile biomolecules survive the attachment process intact. Furthermore, biomolecules must be properly oriented to perform their biological function. In other words, the DNA literally must stand up to be counted. Understanding both the attachment and orientation of DNA on gold surfaces was the goal of recent experiments performed at ALS Beamline 8.0.1 by an international collaboration of scientists.

447

Classifying aging genes into DNA repair or non-DNA repair-related categories  

Science Conference Proceedings (OSTI)

The elderly population in almost every country is growing faster than ever before. However, our knowledge about the aging process is still limited despite decades of studies on this topic. In this report, we focus on the gradual accumulation of DNA damage ... Keywords: DNA-repair, aging, classification, feature selection, random forest

Yaping Fang, Xinkun Wang, Elias K. Michaelis, Jianwen Fang

2013-07-01T23:59:59.000Z

448

Analysis of Two Widespread Versions of a Bacterial Replicative DNA Polymerase  

E-Print Network (OSTI)

DnaE1-pol subunits and the helicase (DnaB). SSB stands forto each polymerase, a (2) helicase for unwinding DNA into

Guenther, Joel Michael

2010-01-01T23:59:59.000Z

449

Structures of Clamp-Loader Complexes Are Key to DNA Replication  

NLE Websites -- All DOE Office Websites (Extended Search)

of various other cellular pathways, such as DNA repair, cell cycle control, and chromatin structure. Sliding DNA clamps are loaded onto DNA by pentameric clamp-loader...

450

Complexation of DNA with Cationic Surfactant  

E-Print Network (OSTI)

Transfection of an anionic polynucleotide through a negatively charged membrane is an important problem in genetic engineering. The direct association of cationic surfactant to DNA decreases the effective negative charge of the nucleic acid, allowing the DNA-surfactant complex to approach a negatively charged membrane. The paper develops a theory for solutions composed of polyelectrolyte, salt, and ionic surfactant. The theoretical predictions are compared with the experimental measurements. PACS.05.70.Ce- Thermodynamic functions and equations of state PACS.61.20.Qg- Structure of associated liquids: electrolytes, molten salts, etc. PACS.61.25.Hq- Macromolecular and polymer solutions; polymer melts; swelling Corresponding author;

Paulo S. Kuhn; Marcia C. Barbosa; Yan Levin

1999-01-01T23:59:59.000Z

451

DNA hybridization : fundamental studies and applications in directed assembly  

E-Print Network (OSTI)

Programmed self-assembly using non-covalent DNA-DNA interactions is a promising technique for the creation of next-generation functional devices for electronic, optical, and magnetic applications. This thesis develops the ...

Bajaj, Manish G. (Manish Gopal)

2005-01-01T23:59:59.000Z

452

DNA Duplication Revealed in New Beginnings | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

DNA Duplication Revealed in New Beginnings DNA Duplication Revealed in New Beginnings DNA Duplication Revealed in New Beginnings April 3, 2012 - 9:36am Addthis The DNA replication origin recognition complex (ORC) is a six-protein machine with a slightly twisted half-ring structure (yellow). ORC is proposed to wrap around and bend approximately 70 base pairs of double stranded DNA (red and blue). When a replication initiator Cdc6 (green) joins ORC, the partial ring is now complete and ready to load another protein onto the DNA. This last protein (not shown) is the enzyme that unwinds the double stranded DNA so each strand can be replicated. | Illustration courtesy of Brookhaven Lab. The DNA replication origin recognition complex (ORC) is a six-protein machine with a slightly twisted half-ring structure (yellow). ORC is

453

Manipulation of cellular DNA repair by early adenovirus proteins  

E-Print Network (OSTI)

of the Bloom's syndrome helicase and its role in recoveryand Ira, G. (2008a). Sgs1 helicase and two nucleases Dna2and Ira, G. (2008b). Sgs1 helicase and two nucleases Dna2

Orazio, Nicole Ise

2010-01-01T23:59:59.000Z

454

Building Energy Software Tools Directory: eDNA  

NLE Websites -- All DOE Office Websites (Extended Search)

many small eDNA systems to be deployed in disparate locations and a central eDNA database (at the summary or enterprise level) can provide access to all of the data from the...

455

Lectin cDNA and transgenic plants derived therefrom  

DOE Patents (OSTI)

Transgenic plants containing cDNA encoding Gramineae lectin are described. The plants preferably contain cDNA coding for barley lectin and store the lectin in the leaves. The transgenic plants, particularly the leaves exhibit insecticidal and fungicidal properties.

Raikhel, Natasha V. (Okemos, MI)

2000-10-03T23:59:59.000Z

456

International congress on DNA damage and repair: Book of abstracts  

SciTech Connect

This document contains the abstracts of 105 papers presented at the Congress. Topics covered include the Escherichia coli nucleotide excision repair system, DNA repair in malignant transformations, defective DNA repair, and gene regulation. (TEM)

Not Available

1987-01-01T23:59:59.000Z

457

Mitigating security issues in the evolving DNA synthesis industry  

E-Print Network (OSTI)

DNA synthesis technologies are advancing at exponential rates, with production of ever longer, more complex, and less expensive sequences of double stranded DNA. This has fostered development of industrial scale design, ...

Turlington, Ralph Donald, III

2013-01-01T23:59:59.000Z

458

Genome scanning : an AFM-based DNA sequencing technique  

E-Print Network (OSTI)

Genome Scanning is a powerful new technique for DNA sequencing. The method presented in this thesis uses an atomic force microscope with a functionalized cantilever tip to sequence single stranded DNA immobilized to a mica ...

Elmouelhi, Ahmed (Ahmed M.), 1979-

2003-01-01T23:59:59.000Z

459

Analysis of the structural changes caused by positive DNA supercoiling  

E-Print Network (OSTI)

The procession of helix-tracking enzymes along a DNA molecule results in the formation of supercoils in the DNA, with positive supercoiling (overwinding) generated ahead of the enzyme, and negative supercoiling (underwinding) ...

Barth, Marita Christine

2007-01-01T23:59:59.000Z

460

Topoisomerase II Structure Suggests Novel DNA Cleavage Mechanism  

NLE Websites -- All DOE Office Websites (Extended Search)

DNA cleavage chemistry used to resolve these altered DNA structures can also operate as a weak link that can be exploited to kill cells-in a good way. A variety of small molecules...

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


461

Nbs1-dependent binding of Mre11 to adenovirus E4 mutant viral DNA is important for inhibiting DNA replication  

Science Conference Proceedings (OSTI)

Adenovirus (Ad) infections stimulate the activation of cellular DNA damage response and repair pathways. Ad early regulatory proteins prevent activation of DNA damage responses by targeting the MRN complex, composed of the Mre11, Rad50 and Nbs1 proteins, for relocalization and degradation. In the absence of these viral proteins, Mre11 colocalizes with viral DNA replication foci. Mre11 foci formation at DNA damage induced by ionizing radiation depends on the Nbs1 component of the MRN complex and is stabilized by the mediator of DNA damage checkpoint protein 1 (Mdc1). We find that Nbs1 is required for Mre11 localization at DNA replication foci in Ad E4 mutant infections. Mre11 is important for Mdc1 foci formation in infected cells, consistent with its role as a sensor of DNA damage. Chromatin immunoprecipitation assays indicate that both Mre11 and Mdc1 are physically bound to viral DNA, which could account for their localization in viral DNA containing foci. Efficient binding of Mre11 to E4 mutant DNA depends on the presence of Nbs1, and is correlated with a significant E4 mutant DNA replication defect. Our results are consistent with a model in which physical interaction of Mre11 with viral DNA is mediated by Nbs1, and interferes with viral DNA replication.

Mathew, Shomita S. [Department of Microbiology, 32 Pearson Hall, Miami University, Oxford OH 45056 (United States); Bridge, Eileen [Department of Microbiology, 32 Pearson Hall, Miami University, Oxford OH 45056 (United States)], E-mail: BridgeE@muohio.edu

2008-04-25T23:59:59.000Z

462

STRBase and Information Resources on Forensic DNA  

E-Print Network (OSTI)

Genetics Information Gathering and Sharing · We live in the information age and need to share what we learn a solid foundation for research and future work #12;Applied Genetics Presentation Outline Information of Journals in Our Group Library We now have on-line access to all forensic DNA journals #12;Applied Genetics

463

Physical approaches to DNA sequencing and detection  

E-Print Network (OSTI)

With the continued improvement of sequencing technologies, the prospect of genome-based medicine is now at the forefront of scientific research. To realize this potential, however, we need a revolutionary sequencing method for the cost-effective and rapid interrogation of individual genomes. This capability is likely to be provided by a physical approach to probing DNA at the single nucleotide level. This is in sharp contrast to current techniques and instruments which probe, through chemical elongation, electrophoresis, and optical detection, length differences and terminating bases of strands of DNA. In this Colloquium we review several physical approaches to DNA detection that have the potential to deliver fast and low-cost sequencing. Center-fold to these approaches is the concept of nanochannels or nanopores which allow for the spatial confinement of DNA molecules. In addition to their possible impact in medicine and biology, the methods offer ideal test beds to study open scientific issues and challenges in the relatively unexplored area at the interface between solids, liquids, and biomolecules at the nanometer length scale. We emphasize the physics behind these methods and ideas, critically describe their advantages and drawbacks, and discuss future research opportunities in this field.

Michael Zwolak; Massimiliano Di Ventra

2007-08-20T23:59:59.000Z

464

Transport and Confinement of DNA within Polymer nano ...  

Science Conference Proceedings (OSTI)

Transport and Confinement of DNA within Polymer nano-tubes. Ana Jofre, Rani Kishore, Kris Helmerson. We have recently ...

465

Studying DNA translocation in nanocapillaries using single molecule fluorescence  

E-Print Network (OSTI)

We demonstrate simultaneous measurements of DNA translocation into glass nanopores using ionic current detection and fluorescent imaging. We verify the correspondence between the passage of a single DNA molecule through the nanopore and the accompanying characteristic ionic current blockage. By tracking the motion of individual DNA molecules in the nanocapillary perpendicular to the optical axis and using a model, we can extract an effective mobility constant for DNA in our geometry under high electric fields.

Thacker, Vivek V; Hernndez-Ainsa, Silvia; Bell, Nicholas A W; Keyser, Ulrich F; 10.1063/1.4768929

2013-01-01T23:59:59.000Z

466

Low-cost, Rapid DNA Sequencing Technique - Oak Ridge ...  

Sequencing DNA is crucial for future breakthroughs in biological and biomedical ... Transportation Sciences UT-Battelle, LLC Oak Ridge National ...

467

Magnetic tweezers to studyMagnetic tweezers to studyMagnetic tweezers to studyMagnetic tweezers to study DNA motorsDNA motorsDNA motorsDNA motors  

E-Print Network (OSTI)

Magnetic tweezers to studyMagnetic tweezers to studyMagnetic tweezers to studyMagnetic tweezers to study DNA motorsDNA motorsDNA motorsDNA motors MariaMariaMariaMaria MañosasMañosasMañosasMañosas RitortCroquetteCroquetteCroquette----BensimonBensimonBensimonBensimon lablablablab ENS FranceENS FranceENS FranceENS France #12;· Introduction to MT (magnetic tweezers

Ritort, Felix

468

RecQ helicase stimulates both DNA catenation and changes in DNA topology by topoisomerase III  

E-Print Network (OSTI)

Passage Activity of RecQ Helicase and Topo III 28. DiGate,2003 Printed in U.S.A. RecQ Helicase Stimulates Both DNA95616 Together, RecQ helicase and topoisomerase III (Topo

Harmon, Frank G; Brockman, Joel P; Kowalczykowski, Stephen C

2003-01-01T23:59:59.000Z

469

DNA ruler : enhancing nanopore sizing resolution by multiple measurements on the same DNA molecule  

E-Print Network (OSTI)

Nanopores are versatile sensors for label-free detection of single molecules and particles that have attracted attention for applications such as DNA sequencing and nanoparticle analysis. Detection of single molecules or ...

Sen, Yi-Heng

2012-01-01T23:59:59.000Z

470

EST_GENOME: a program to align spliced DNA sequences to unspliced genomic DNA  

E-Print Network (OSTI)

spliced DNA to unspliced genomic DNA. It is written in ANSI C and has been tested under Digital OSF3.2. The spurce code and documentation are available from ftp:// www.sanger.ac.uky ftp/pub / badger/est_genome.2.tar.Z. The prediction of genes in uncharacterized genomic DNA sequence is currently one of the main problems facing sequence annotators. Methods based on de novo prediction, e.g. searching for motifs like the splice-site consensus, or on statistical properties such as biased codon usage, etc. (Solovyev et al., 1994; Hebsgaard et al., 1996) have been only partially successful, and investigators have often found that the surest way of predicting a gene is by alignment with a homologous protein sequence (Birney et al., 1996; Gelfand et al., 1996; Huang and Zhang, 1996), or a spliced gene product [an expressed sequence tag (EST), mRNA or cDNA],

Richard Mott

1997-01-01T23:59:59.000Z

471

NMR structure of the N-terminal domain of the replication initiator protein DnaA  

E-Print Network (OSTI)

recruitment of the DnaBC helicase, and the assembly of theDnaA then recruits the DnaB helicase with help from DnaC to

Lowery, Thomas J.

2008-01-01T23:59:59.000Z

472

DNA fragment sizing and sorting by laser-induced fluorescence  

DOE Patents (OSTI)

A method is provided for obtaining DNA fingerprints using high speed detection systems, such as flow cytometry to determine unique characteristics of DNA pieces from a selected sample. In one characterization the DNA piece is fragmented at preselected sites to produce a plurality of DNA fragments. The DNA piece or the resulting DNA fragments are treated with a dye effective to stain stoichiometrically the DNA fragments. The fluorescence from the dye in the stained fragments is then examined to generate an output functionally related to the number of nucleotides in each one of the DNA fragments. In one embodiment, the intensity of the fluorescence emissions from each fragment is directly proportional to the fragment length. Additional dyes can be bound to the DNA piece and DNA fragments to provide information additional to length information. Oligonucleotide specific dyes and/or hybridization probes can be bound to the DNA fragments to provide information on oligonucleotide distribution or probe hybridization to DNA fragments of different sizes.

Jett, J.H.; Hammond, M.L.; Keller, R.A.; Marrone, B.L.; Martin, J.C.

1992-12-31T23:59:59.000Z

473

Fuzzy C-Means Based DNA Motif Discovery  

Science Conference Proceedings (OSTI)

In this paper, we examined the problem of identifying motifs in DNA sequences. Transcription-binding sites, which are functionally significant subsequences, are considered as motifs. In order to reveal such DNA motifs, our method makes use of Fuzzy clustering ... Keywords: DNA Sequences, Fuzzy Clustering, Motif Finding

Mustafa Karabulut; Turgay Ibrikci

2008-09-01T23:59:59.000Z

474

Mutations Altering the Interplay between GkDnaC Helicase and DNA Reveal an Insight into Helicase Unwinding  

E-Print Network (OSTI)

Replicative helicases are essential molecular machines that utilize energy derived from NTP hydrolysis to move along nucleic acids and to unwind double-stranded DNA (dsDNA). Our earlier crystal structure of the hexameric helicase from Geobacillus kaustophilus HTA426 (GkDnaC) in complex with single-stranded DNA (ssDNA) suggested several key residues responsible for DNA binding that likely play a role in DNA translocation during the unwinding process. Here, we demonstrated that the unwinding activities of mutants with substitutions at these key residues in GkDnaC are 24-fold higher than that of wildtype protein. We also observed the faster unwinding velocities in these mutants using single-molecule experiments. A partial loss in the interaction of helicase with ssDNA leads to an enhancement in helicase efficiency, while their ATPase activities remain unchanged. In strong contrast, adding accessory proteins (DnaG or DnaI) to GkDnaC helicase alters the ATPase, unwinding efficiency and the unwinding velocity of the helicase. It suggests that the unwinding velocity of helicase

Yu-hua Lo; Shih-wei Liu; Yuh-ju Sun; Hung-wen Lizz; Chwan-deng Hsiao

2011-01-01T23:59:59.000Z

475

Self-assembled DNA Nanostructures and DNA Devices John Reif Harish Chandran Nikhil Gopalkrishnan Thomas LaBean  

E-Print Network (OSTI)

and complexity. We discuss the design and demonstration of molecular-scale devices that make use of DNA, error-free methods for self-assembly of complex devices out of large number of molecular componentsSelf-assembled DNA Nanostructures and DNA Devices John Reif Harish Chandran Nikhil Gopalkrishnan

Reif, John H.

476

Protein Activity that Protects Our DNA - Research Highlights | ORNL Neutron  

NLE Websites -- All DOE Office Websites (Extended Search)

Neutrons help shed light on critical protein activity that protects our DNA Neutrons help shed light on critical protein activity that protects our DNA "New study provides a framework for understanding how protein works and how it stimulates the DNA processing machines" Research Contact: Walter Chazin Illustration of the change in architecture of the essential eukaryotic ssDNA binding protein RPA as it engages progressively longer segments of ssDNA. Small-angle x-ray scattering data are displayed in the background for the DNA binding core of RPA in its DNA-free state (green) and when engaged on 10 (yellow), 20 (red, and 30 (blue) nucleotide ssDNA substrates. Overlaid molecular surfaces and ribbon representations of the three distinct architectural states of RPA are shown, one for the DNA-free protein and the two others for the initial and fully ssDNA-engaged modes, revealing the progressive compaction of the protein as it binds to the substrate. The RPA70 subunit is colored in blue, RPA32 in green, and RPA14 in red, with ssDNA displayed as a yellow ribbon.

477

When DNA Needs to Stand Up and Be Counted  

NLE Websites -- All DOE Office Websites (Extended Search)

When DNA Needs to Stand Up and When DNA Needs to Stand Up and Be Counted When DNA Needs to Stand Up and Be Counted Print Wednesday, 31 May 2006 00:00 DNA microarrays are small metal, glass, or silicon chips covered with patterns of short single-stranded DNA (ssDNA). These "DNA chips" are revolutionizing biotechnology, allowing scientists to identify and count many DNA sequences simultaneously. They are the enabling technology for genomic-based medicine and are a critical component of advanced diagnostic systems for medical and homeland security applications. Like digital chips, DNA chips are parallel, accurate, fast, and small. These advantages, however, can only be realized if the fragile biomolecules survive the attachment process intact. Furthermore, biomolecules must be properly oriented to perform their biological function. In other words, the DNA literally must stand up to be counted. Understanding both the attachment and orientation of DNA on gold surfaces was the goal of recent experiments performed at ALS Beamline 8.0.1 by an international collaboration of scientists.

478

Topoisomerase II Structure Suggests Novel DNA Cleavage Mechanism  

NLE Websites -- All DOE Office Websites (Extended Search)

Topoisomerase II Structure Suggests Novel DNA Cleavage Mechanism Print Topoisomerase II Structure Suggests Novel DNA Cleavage Mechanism Print Type II topoisomerases are molecular machines that regulate DNA supercoiling and separate interlocked chromosomes. These enzymes are also exploited clinically as targets of antibiotics and anticancer therapeutics. Researchers at ALS Beamline 8.3.1 imaged type II topoisomerase's ordinarily short-lived state in which it is linked to a DNA's nucleic acid segment through its active site tyrosine, cleaving the DNA. Details of this molecular model provide evidence for the chemical mechanism by which type II topoisomerases (topo IIs) and a related topo family (topo IA) accomplish DNA cleavage. The structure also reveals how the enzyme avoids dissociating when DNA is cleaved, preventing the aberrant formation of mutagenic genomic lesions.

479

Protein Bridges DNA Base and Nucleotide Excision Repair Pathways  

NLE Websites -- All DOE Office Websites (Extended Search)

Protein Bridges DNA Base and Protein Bridges DNA Base and Nucleotide Excision Repair Pathways Protein Bridges DNA Base and Nucleotide Excision Repair Pathways Print Wednesday, 28 October 2009 00:00 Alkyltransferase proteins (AGT) protect cells from the biological effects of DNA damage caused by the addition of alkyl groups (alkylation). Alkyltransferase-like proteins (ATLs) can do the same, but they lack the reactive cysteine residue that allows the alkyltransferase function, and the mechanism for cell protection has remained unknown. To address this mystery, a British-American team lead by researchers at the Scripps Research Institute recently applied a combination of x-ray structural, biochemical, and genetic studies to ATLs in the yeast Schizosaccharomyces pombe without and with damaged DNA. By showing how a process called non-enzymatic nucleotide flipping activates ATL-initiated DNA repair, their results may improve our understanding of genomic integrity and responses to DNA damage relevant to pathogens and cancer development.

480

Low Dose Radiation Research Program: Assessing Biological Function of DNA  

NLE Websites -- All DOE Office Websites (Extended Search)

Assessing Biological Function of DNA Damage Response Genes Assessing Biological Function of DNA Damage Response Genes Larry H. Thompson Lawrence Livermore National Laboratory Why This Project To understand the relative importance of individual DNA repair and DNA-damage response pathways to the recovery of mammalian cells after exposure to low doses of ionizing radiation (IR). This understanding may lead to better ways of setting limits on human exposure to IR. In spite of the discovery of many mammalian DNA repair genes, our current knowledge of how many of these genes contribute to cellular recovery from IR exposure is quite limited. Project Goals Measure cellular responses at doses in the 5-100 cGy range, which generally cause changes too small to detect in normal, repair-proficient cells Focus on DNA double-strand breaks (DSBs) and DNA oxidative base

Note: This page contains sample records for the topic "dna lightning dock" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


481

Topoisomerase II Structure Suggests Novel DNA Cleavage Mechanism  

NLE Websites -- All DOE Office Websites (Extended Search)

Topoisomerase II Structure Suggests Novel DNA Cleavage Mechanism Print Topoisomerase II Structure Suggests Novel DNA Cleavage Mechanism Print Type II topoisomerases are molecular machines that regulate DNA supercoiling and separate interlocked chromosomes. These enzymes are also exploited clinically as targets of antibiotics and anticancer therapeutics. Researchers at ALS Beamline 8.3.1 imaged type II topoisomerase's ordinarily short-lived state in which it is linked to a DNA's nucleic acid segment through its active site tyrosine, cleaving the DNA. Details of this molecular model provide evidence for the chemical mechanism by which type II topoisomerases (topo IIs) and a related topo family (topo IA) accomplish DNA cleavage. The structure also reveals how the enzyme avoids dissociating when DNA is cleaved, preventing the aberrant formation of mutagenic genomic lesions.

482

Topoisomerase II Structure Suggests Novel DNA Cleavage Mechanism  

NLE Websites -- All DOE Office Websites (Extended Search)

Topoisomerase II Structure Suggests Novel DNA Cleavage Mechanism Print Topoisomerase II Structure Suggests Novel DNA Cleavage Mechanism Print Type II topoisomerases are molecular machines that regulate DNA supercoiling and separate interlocked chromosomes. These enzymes are also exploited clinically as targets of antibiotics and anticancer therapeutics. Researchers at ALS Beamline 8.3.1 imaged type II topoisomerase's ordinarily short-lived state in which it is linked to a DNA's nucleic acid segment through its active site tyrosine, cleaving the DNA. Details of this molecular model provide evidence for the chemical mechanism by which type II topoisomerases (topo IIs) and a related topo family (topo IA) accomplish DNA cleavage. The structure also reveals how the enzyme avoids dissociating when DNA is cleaved, preventing the aberrant formation of mutagenic genomic lesions.

483

Topoisomerase II Structure Suggests Novel DNA Cleavage Mechanism  

NLE Websites -- All DOE Office Websites (Extended Search)

Topoisomerase II Structure Topoisomerase II Structure Suggests Novel DNA Cleavage Mechanism Topoisomerase II Structure Suggests Novel DNA Cleavage Mechanism Print Wednesday, 26 January 2011 00:00 Type II topoisomerases are molecular machines that regulate DNA supercoiling and separate interlocked chromosomes. These enzymes are also exploited clinically as targets of antibiotics and anticancer therapeutics. Researchers at ALS Beamline 8.3.1 imaged type II topoisomerase's ordinarily short-lived state in which it is linked to a DNA's nucleic acid segment through its active site tyrosine, cleaving the DNA. Details of this molecular model provide evidence for the chemical mechanism by which type II topoisomerases (topo IIs) and a related topo family (topo IA) accomplish DNA cleavage. The structure also reveals how the enzyme avoids dissociating when DNA is cleaved, preventing the aberrant formation of mutagenic genomic lesions.

484

Mechanism of DNA Translocation in a Replicative Hexameric Helicase  

Science Conference Proceedings (OSTI)

The E1 protein of papillomavirus is a hexameric ring helicase belonging to the AAA + family. The mechanism that couples the ATP cycle to DNA translocation has been unclear. Here we present the crystal structure of the E1 hexamer with single-stranded DNA discretely bound within the hexamer channel and nucleotides at the subunit interfaces. This structure demonstrates that only one strand of DNA passes through the hexamer channel and that the DNA-binding hairpins of each subunit form a spiral 'staircase' that sequentially tracks the oligonucleotide backbone. Consecutively grouped ATP, ADP and apo configurations correlate with the height of the hairpin, suggesting a straightforward DNA translocation mechanism. Each subunit sequentially progresses through ATP, ADP and apo states while the associated DNA-binding hairpin travels from the top staircase position to the bottom, escorting one nucleotide of single-stranded DNA through the channel. These events permute sequentially around the ring from one subunit to the next.

Enemark,E.; Joshua-Tor, L.

2006-01-01T23:59:59.000Z

485

Sequencing Intractable DNA to Close Microbial Genomes  

Science Conference Proceedings (OSTI)

Advancement in high throughput DNA sequencing technologies has supported a rapid proliferation of microbial genome sequencing projects, providing the genetic blueprint for for in-depth studies. Oftentimes, difficult to sequence regions in microbial genomes are ruled intractable resulting in a growing number of genomes with sequence gaps deposited in databases. A procedure was developed to sequence such difficult regions in the non-contiguous finished Desulfovibrio desulfuricans ND132 genome (6 intractable gaps) and the Desulfovibrio africanus genome (1 intractable gap). The polynucleotides surrounding each gap formed GC rich secondary structures making the regions refractory to amplification and sequencing. Strand-displacing DNA polymerases used in concert with a novel ramped PCR extension cycle supported amplification and closure of all gap regions in both genomes. These developed procedures support accurate gene annotation, and provide a step-wise method that reduces the effort required for genome finishing.

Hurt, Jr., Richard Ashley [ORNL; Brown, Steven D [ORNL; Podar, Mircea [ORNL; Palumbo, Anthony Vito [ORNL; Elias, Dwayne A [ORNL

2012-01-01T23:59:59.000Z

486

Fast DNA Sequencing via Transverse Electronic Transport  

E-Print Network (OSTI)

A rapid and low-cost method to sequence DNA would usher in a revolution in medicine. We propose and theoretically show the feasibility of a protocol for sequencing based on the distributions of transverse electrical currents of single-stranded DNA while it translocates through a nanopore. Our estimates, based on the statistics of these distributions, reveal that sequencing of an entire human genome could be done with very high accuracy in a matter of hours without parallelization, e.g., orders of magnitude faster than present techniques. The practical implementation of our approach would represent a substantial advancement in our ability to study, predict and cure diseases from the perspective of the genetic makeup of each individual.

Johan Lagerqvist; Michael Zwolak; Massimiliano Di Ventra

2006-01-18T23:59:59.000Z

487

Organic Data Memory using the DNA Approach  

Science Conference Proceedings (OSTI)

A data preservation problem looms large behind today's information superhighway. During the prehistoric age, humans preserved their knowledge by engraving bones and rocks. About two millenniums ago, people invented paper and started writing and publishing. In today?s electronic age, we use magnetic media and silicon chips to store our data. But bones and rocks erode, paper disintegrates, and electronic memory simply loses its contents into thin air. All these storage media require constant attention to maintain their information content. All of them can easily be destroyed intentionally or accidentally by people or natural disasters. With the large amount of information generated by our society every day, it is time to think of a new generation of data memory. In an effort to search for an inexpensive and lasting data memory, scientists at the Pacific Northwest National Laboratory (PNNL) have investigated the use of deoxyribonucleic acid--commonly known as DNA--as an information storage medium since 1998. The ambitious goal is to develop a data memory technology that has a life expectancy much longer than any of the existing ones. The creation of our initial DNA memory prototype consists of four major steps: encoding meaningful information as artificial DNA sequences, transforming the sequences to living organisms, allowing the organisms to grow and multiply, and eventually extracting the information back from the organisms. This article describes the objective of our investigation, followed by a brief description of our recent experiments and several potential applications being considered at PNNL.

Wong, Pak C.; Wong, Kwong K.; Foote, Harlan P.

2003-01-01T23:59:59.000Z

488

Phylogenetic Analysis of Shewanella Strains by DNA Relatedness Derived from Whole Genome Microarray DNA-DNA Hybridization and Comparison with Other Methods  

SciTech Connect

Phylogenetic analyses were done for the Shewanella strains isolated from Baltic Sea (38 strains), US DOE Hanford Uranium bioremediation site [Hanford Reach of the Columbia River (HRCR), 11 strains], Pacific Ocean and Hawaiian sediments (8 strains), and strains from other resources (16 strains) with three out group strains, Rhodopseudomonas palustris, Clostridium cellulolyticum, and Thermoanaerobacter ethanolicus X514, using DNA relatedness derived from WCGA-based DNA-DNA hybridizations, sequence similarities of 16S rRNA gene and gyrB gene, and sequence similarities of 6 loci of Shewanella genome selected from a shared gene list of the Shewanella strains with whole genome sequenced based on the average nucleotide identity of them (ANI). The phylogenetic trees based on 16S rRNA and gyrB gene sequences, and DNA relatedness derived from WCGA hybridizations of the tested Shewanella strains share exactly the same sub-clusters with very few exceptions, in which the strains were basically grouped by species. However, the phylogenetic analysis based on DNA relatedness derived from WCGA hybridizations dramatically increased the differentiation resolution at species and strains level within Shewanella genus. When the tree based on DNA relatedness derived from WCGA hybridizations was compared to the tree based on the combined sequences of the selected functional genes (6 loci), we found that the resolutions of both methods are similar, but the clustering of the tree based on DNA relatedness derived from WMGA hybridizations was clearer. These results indicate that WCGA-based DNA-DNA hybridization is an idea alternative of conventional DNA-DNA hybridization methods and it is superior to the phylogenetics methods based on sequence similarities of single genes. Detailed analysis is being performed for the re-classification of the strains examined.

Wu, Liyou; Yi, T. Y.; Van Nostrand, Joy; Zhou, Jizhong

2010-05-17T23:59:59.000Z

489

Intragenic and extragenic suppressors of temperature sensitive mutations replication initiation genes dnaD and dnaB of Bacillus subtilis  

E-Print Network (OSTI)

Background: The Bacillus subtilis genes dnaD and dnaB are essential for the initiation of DNA replication and are required for loading of the replicative helicase at the chromosomal origin of replication oriC. Wild type ...

Grossman, Alan D.

490

Weakly Charged Cationic Nanoparticles Induce DNA Bending and Strand Separation  

SciTech Connect

The understanding of interactions between double stranded (ds) DNA and charged nanoparticles will have a broad bearing on many important applications from drug delivery [ 1 4 ] to DNAtemplated metallization. [ 5 , 6 ] Cationic nanoparticles (NPs) can bind to DNA, a negatively charged molecule, through a combination of electrostatic attraction, groove binding, and intercalation. Such binding events induce changes in the conformation of a DNA strand. In nature, DNA wraps around a cylindrical protein assembly (diameter and height of 6 nm) [ 7 ] with an 220 positive charge, [ 8 ] creating the complex known as chromatin. Wrapping and bending of DNA has also been achieved in the laboratory through the binding of highly charged species such as molecular assemblies, [ 9 , 10 ] cationic dendrimers, [ 11 , 12 ] and nanoparticles. [ 13 15 ] The charge of a nanoparticle plays a crucial role in its ability to induce DNA structural changes. If a nanoparticle has a highly positive surface charge density, the DNA is likely to wrap and bend upon binding to the nanoparticle [ 13 ] (as in the case of chromatin). On the other hand, if a nanoparticle is weakly charged it will not induce dsDNA compaction. [ 9 , 10 , 15 ] Consequently, there is a transition zone from extended to compact DNA conformations which depends on the chemical nature of the nanoparticle and occurs for polycations with charges between 5 and 10. [ 9 ] While the interactions between highly charged NPs and DNA have been extensively studied, the processes that occur within the transition zone are less explored.

Railsback, Justin [North Carolina State University; Singh, Abhishek [North Carolina State University; Pearce, Ryan [North Carolina State University; McKnight, Timothy E [ORNL; Collazo, Ramon [North Carolina State University; Sitar, Zlatko [ORNL; Yingling, Yaroslava [North Carolina State University; Melechko, Anatoli Vasilievich [ORNL

2012-01-01T23:59:59.000Z

491

DNA repair decline during mouse spermiogenesis results in the accumulation of heritable DNA damage  

Science Conference Proceedings (OSTI)

The post-meiotic phase of mouse spermatogenesis (spermiogenesis) is very sensitive to the genomic effects of environmental mutagens because as male germ cells form mature sperm they progressively lose the ability to repair DNA damage. We hypothesized that repeated exposures to mutagens during this repair-deficient phase result in the accumulation of heritable genomic damage in mouse sperm that leads to chromosomal aberrations in zygotes after fertilization. We used a combination of single or fractionated exposures to diepoxybutane (DEB), a component of tobacco smoke, to investigate how differential DNA repair efficiencies during the three weeks of spermiogenesis affected the accumulation of DEB-induced heritable damage in early spermatids (21-15 days before fertilization, dbf), late spermatids (14-8 dbf) and sperm (7- 1 dbf). Analysis of chromosomalaberrations in zygotic metaphases using PAINT/DAPI showed that late spermatids and sperm are unable to repair DEB-induced DNA damage as demonstrated by significant increases (P<0.001) in the frequencies of zygotes with chromosomal aberrations. Comparisons between single and fractionated exposures suggested that the DNA repair-deficient window during late spermiogenesis may be less than two weeks in the mouse and that during this repair-deficient window there is accumulation of DNA damage in sperm. Finally, the dose-response study in sperm indicated a linear response for both single and repeated exposures. These findings show that the differential DNA repair capacity of post-meioitic male germ cells has a major impact on the risk of paternally transmitted heritable damage and suggest that chronic exposures that may occur in the weeks prior to fertilization because of occupational or lifestyle factors (i.e, smoking) can lead to an accumulation of genetic damage in sperm and result in heritable chromosomal aberrations of paternal origin.

Marchetti, Francesco; Marchetti, Francesco; Wryobek, Andrew J

2008-02-21T23:59:59.000Z

492

DNA Repair Decline During Mouse Spermiogenesis Results in the Accumulation of Heritable DNA Damage  

Science Conference Proceedings (OSTI)

The post-meiotic phase of mouse spermatogenesis (spermiogenesis) is very sensitive to the genomic effects of environmental mutagens because as male germ cells form mature sperm they progressively lose the ability to repair DNA damage. We hypothesized that repeated exposures to mutagens during this repair-deficient phase result in the accumulation of heritable genomic damage in mouse sperm that leads to chromosomal aberrations in zygotes after fertilization. We used a combination of single or fractionated exposures to diepoxybutane (DEB), a component of tobacco smoke, to investigate how differential DNA repair efficiencies during the three weeks of spermiogenesis affected the accumulation of DEB-induced heritable damage in early spermatids (21-15 days before fertilization, dbf), late spermatids (14-8 dbf) and sperm (7-1 dbf). Analysis of chromosomal aberrations in zygotic metaphases using PAINT/DAPI showed that late spermatids and sperm are unable to repair DEB-induced DNA damage as demonstrated by significant increases (P<0.001) in the frequencies of zygotes with chromosomal aberrations. Comparisons between single and fractionated exposures suggested that the DNA repair-deficient window during late spermiogenesis may be less than two weeks in the mouse and that during this repair-deficient window there is accumulation of DNA damage in sperm. Finally, the dose-response study in sperm indicated a linear response for both single and repeated exposures. These findings show that the differential DNA repair capacity of post-meioitic male germ cells has a major impact on the risk of paternally transmitted heritable damage and suggest that chronic exposures that may occur in the weeks prior to fertilization because of occupational or lifestyle factors (i.e, smoking) can lead to an accumulation of genetic damage in sperm and result in heritable chromosomal aberrations of paternal origin.

Marchetti, Francesco; Marchetti, Francesco; Wyrobek, Andrew J.

2007-12-01T23:59:59.000Z

493

Unraveling the Fanconi anaemia-DNA repair connection through DNA helicase and translocase activities  

Science Conference Proceedings (OSTI)

How the Fanconi anaemia (FA) chromosome stability pathway functions to cope with interstrand crosslinks and other DNA lesions has been elusive, even after FANCD1 proved to be BRCA2, a partner of Rad51 in homologous recombination. The identification and characterization of two new Fanconi proteins having helicase motifs, FANCM and FANCJ/BRIP1/BACH1, implicates the FANC nuclear core complex as a participant in recognizing or processing damaged DNA, and the BRIP1 helicase as acting independently of this complex.

Thompson, L H

2005-08-16T23:59:59.000Z