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Note: This page contains sample records for the topic "dna double-strand breaks" from the National Library of EnergyBeta (NLEBeta).
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1

Processing of 3'-Phosphoglycolate-Terminated DNA Double-StrandBreaks by Artemis Nuclease  

SciTech Connect (OSTI)

The Artemis nuclease is required for V(D)J recombination and for repair of an as yet undefined subset of radiation-induced DNA double-strand breaks. To assess the possibility that Artemis functions on oxidatively modified double-strand break termini, its activity toward model DNA substrates, bearing either 3{prime}-hydroxyl or 3{prime}-phosphoglycolate moieties, was examined. A 3{prime}-phosphoglycolate had little effect on Artemis-mediated trimming of long 3{prime} overhangs (>9 nucleotides), which were efficiently trimmed to 4-5 nucleotides. However, 3{prime}-phosphoglycolates on overhangs of 4-5 bases promoted selective Artemis-mediated trimming of a single 3{prime}-terminal nucleotide, while at least 2 nucleotides were trimmed from identical hydroxyl-terminated substrates. Artemis also efficiently removed a single nucleotide from a phosphoglycolate-terminated 3-base 3{prime} overhang, while leaving an analogous hydroxyl-terminated overhang largely intact. Such removal was dependent upon Ku, DNA-dependent protein kinase, and ATP. Together, these data suggest that Artemis-mediated cleavage of 3{prime} overhangs requires a minimum of 2 nucleotides, or a nucleotide plus a phosphoglycolate, 3{prime} to the cleavage site. Shorter 3{prime}-phosphoglycolate-terminated overhangs and blunt ends were also processed by Artemis, but much less efficiently. Consistent with the in vitro substrate specificity of Artemis, human cells lacking Artemis exhibited hypersensitivity to X-rays, bleomycin and neocarzinostatin, which all induce 3{prime}-phosphoglycolate-terminated double-strand breaks. Collectively, these results suggest that 3{prime}-phosphoglycolate termini and/or specific classes of DNA ends that arise from such blocked termini are relevant Artemis substrates in vivo.

Povrik, Lawrence F.; Zhou, Tong; Zhou, Ruizhe; Cowan, Morton J.; Yannone, Steven M.

2005-10-01T23:59:59.000Z

2

Telomeres and DNA double-strand breaks: ever the twain shall S. M. Baileya,* and M. N. Cornforthb  

E-Print Network [OSTI]

between the naturally occurring DNA double-strand breaks (DSBs) found at chromosome ends (i.e., telomeres Collins, CO 80523-1618 (USA), Fax: +19704917742, e-mail: sbailey@colostate.edu b Department of Radiation on their inability to rejoin with broken chromosome ends produced by radiation. Today, we recognize two essential

3

Detection and Repair of Ionizing Radiation-Induced DNA Double Strand Breaks: New Developments in Nonhomologous End Joining  

SciTech Connect (OSTI)

DNA damage can occur as a result of endogenous metabolic reactions and replication stress or from exogenous sources such as radiation therapy and chemotherapy. DNA double strand breaks are the most cytotoxic form of DNA damage, and defects in their repair can result in genome instability, a hallmark of cancer. The major pathway for the repair of ionizing radiation-induced DSBs in human cells is nonhomologous end joining. Here we review recent advances on the mechanism of nonhomologous end joining, as well as new findings on its component proteins and regulation.

Wang, Chen [Departments of Biochemistry and Molecular Biology and Oncology, and Southern Alberta Cancer Research Institute, University of Calgary, Calgary (Canada)] [Departments of Biochemistry and Molecular Biology and Oncology, and Southern Alberta Cancer Research Institute, University of Calgary, Calgary (Canada); Lees-Miller, Susan P., E-mail: leesmill@ucalgary.ca [Departments of Biochemistry and Molecular Biology and Oncology, and Southern Alberta Cancer Research Institute, University of Calgary, Calgary (Canada)

2013-07-01T23:59:59.000Z

4

Probability of double-strand breaks in genome-sized DNA by {gamma}-ray decreases markedly as the DNA concentration increases  

SciTech Connect (OSTI)

By use of the single-molecule observation, we count the number of DNA double-strand breaks caused by {gamma}-ray irradiation with genome-sized DNA molecules (166 kbp). We find that P{sub 1}, the number of double-strand breaks (DSBs) per base pair per unit Gy, is nearly inversely proportional to the DNA concentration above a certain threshold DNA concentration. The inverse relationship implies that the total number of DSBs remains essentially constant. We give a theoretical interpretation of our experimental results in terms of attack of reactive species upon DNA molecules, indicating the significance of the characteristics of genome-sized giant DNA as semiflexible polymers for the efficiency of DSBs.

Shimobayashi, Shunsuke F. [Department of Physics, Graduate School of Science, Kyoto University, Kyoto 606-8502 (Japan); Iwaki, Takafumi [Fukui Institute for Fundamental Chemistry, Kyoto University, Kyoto 606-8103 (Japan); Mori, Toshiaki [Radiation Research Center, Osaka Prefecture University, Sakai 599-8570 (Japan); Yoshikawa, Kenichi [Department of Physics, Graduate School of Science, Kyoto University, Kyoto 606-8502 (Japan); Faculty of Life and Medical Sciences, Doshisha University, Kyoto 610-0394 (Japan)

2013-05-07T23:59:59.000Z

5

Coordinateendonucleolytic 5' and 3' trimming of terminally blocked blunt DNA double-strand break ends by Artemis nuclease and DNA-dependent protein kinase  

SciTech Connect (OSTI)

Previous work showed that, in the presence of DNA-PK, Artemis slowly trims 3'-phosphoglycolate-terminated blunt ends. To examine the trimming reaction in more detail, long internally labeled DNA substrates were treated with Artemis. In the absence of DNA-PK, Artemis catalyzed extensive 5' {yields} 3' exonucleolytic resection of double-stranded DNA. This resection required a 5'-phosphate but did not require ATP, and was accompanied by endonucleolytic cleavage of the resulting 3' overhang. In the presence of DNA-PK, Artemis-mediated trimming was more limited, was ATP-dependent, and did not require a 5'-phosphate. For a blunt end with either a 3'-phosphoglycolate or 3'-hydroxyl terminus, endonucleolytic trimming of 2-4 nucleotides from the 3'-terminal strand was accompanied by trimming of 6 nucleotides from the 5'-terminal strand. The results suggest that autophosphorylated DNA-PK suppresses the exonuclease activity of Artemis toward blunt-ended DNA, and promotes slow and limited endonucleolytic trimming of the 5'-terminal strand, resulting in short 3' overhangs that are trimmed endonucleolytically. Thus, Artemis and DNA-PK can convert terminally blocked DNA ends of diverse geometry and chemical structure to a form suitable for polymerase mediated patching and ligation, with minimal loss of terminal sequence. Such processing could account for the very small deletions often found at DNA double-strand break repair sites.

Povirk, Lawrence; Yannone, Steven M.; Khan, Imran S.; Zhou, Rui-Zhe; Zhou, Tong; Valerie, Kristoffer; F., Lawrence

2008-02-18T23:59:59.000Z

6

PAXX, a paralog of XRCC4 and XLF, interacts with Ku to promote DNA double-strand break repair  

E-Print Network [OSTI]

regions of PAXX1-204 and PAXXV199A/F201A were amplified from the vectors by PCR and cloned into the pcDNA3.1(-) vector (a gift from Dr. V. Bolanos-Garcia) together with GFP or FLAG tags using In-Fusion (Clontech). Vectors were sequenced by the DNA... -treated or treated for 1 hour with 300 µM phleomycin to induce large numbers of DSBs, before being harvested either at this point or after an additional 1-hour recovery period in phleomycin-free medium after three PBS washes. Cells were washed twice in ice-cold PBS...

Ochi, Takashi; Blackford, Andrew N.; Coates, Julia; Jhujh, Satpal; Mehmood, Shahid; Tamura, Naoka; Travers, Jon; Wu, Qian; Draviam, Viji M.; Robinson, Carol V.; Blundell, Tom L.; Jackson, Stephen P.

2015-01-09T23:59:59.000Z

7

Homologous recombination contributes to the repair of DNA double-strand breaks induced by high-energy iron ions  

SciTech Connect (OSTI)

To test the contribution of homologous recombinational repair (HRR) in repairing DNA damaged sites induced by high-energy iron ions, we used: (1) HRR-deficient rodent cells carrying a deletion in the RAD51D gene and (2) syngeneic human cells impaired for HRR by RAD51D or RAD51 knockdown using RNA interference. We show that in response to iron ions, HRR contributes to cell survival in rodent cells, and that HRR-deficiency abrogates RAD51 foci formation. Complementation of the HRR defect by human RAD51D rescues both enhanced cytotoxicity and RAD51 foci formation. For human cells irradiated with iron ions, cell survival is decreased, and, in p53 mutant cells, the levels of mutagenesis are increased when HRR is impaired. Human cells synchronized in S phase exhibit more pronounced resistance to iron ions as compared with cells in G1 phase, and this increase in radioresistance is diminished by RAD51 knockdown. These results implicate a role for RAD51-mediated DNA repair (i.e. HRR) in removing a fraction of clustered lesions induced by charged particle irradiation. Our results are the first to directly show the requirement for an intact HRR pathway in human cells in ensuring DNA repair and cell survival in response to high-energy high LET radiation.

Zafar, Faria; Seidler, Sara B.; Kronenberg, Amy; Schild, David; Wiese, Claudia

2010-06-29T23:59:59.000Z

8

Induction and Rejoining of DNA Double Strand Breaks Assessed by H2AX Phosphorylation in Melanoma Cells Irradiated with Proton and Lithium Beams  

SciTech Connect (OSTI)

Purpose: The aim of this study was to evaluate the induction and rejoining of DNA double strand breaks (DSBs) in melanoma cells exposed to low and high linear energy transfer (LET) radiation. Methods and Materials: DSBs and survival were determined as a function of dose in melanoma cells (B16-F0) irradiated with monoenergetic proton and lithium beams and with a gamma source. Survival curves were obtained by clonogenic assay and fitted to the linear-quadratic model. DSBs were evaluated by the detection of phosphorylated histone H2AX ({gamma}H2AX) foci at 30 min and 6 h post-irradiation. Results: Survival curves showed the increasing effectiveness of radiation as a function of LET. {gamma}H2AX labeling showed an increase in the number of foci vs. dose for all the radiations evaluated. A decrease in the number of foci was found at 6 h post-irradiation for low LET radiation, revealing the repair capacity of DSBs. An increase in the size of {gamma}H2AX foci in cells irradiated with lithium beams was found, as compared with gamma and proton irradiations, which could be attributed to the clusters of DSBs induced by high LET radiation. Foci size increased at 6 h post-irradiation for lithium and proton irradiations in relation with persistent DSBs, showing a correlation with surviving fraction. Conclusions: Our results showed the response of B16-F0 cells to charged particle beams evaluated by the detection of {gamma}H2AX foci. We conclude that {gamma}H2AX foci size is an accurate parameter to correlate the rejoining of DSBs induced by different LET radiations and radiosensitivity.

Ibanez, Irene L. [Comision Nacional de Energia Atomica, San Martin (Argentina); Consejo Nacional de Investigaciones Cientificas y Tecnicas, Buenos Aires (Argentina); Bracalente, Candelaria [Comision Nacional de Energia Atomica, San Martin (Argentina); Molinari, Beatriz L. [Comision Nacional de Energia Atomica, San Martin (Argentina); Consejo Nacional de Investigaciones Cientificas y Tecnicas, Buenos Aires (Argentina); Palmieri, Monica A. [Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires (Argentina); Policastro, Lucia [Comision Nacional de Energia Atomica, San Martin (Argentina); Consejo Nacional de Investigaciones Cientificas y Tecnicas, Buenos Aires (Argentina); Kreiner, Andres J. [Comision Nacional de Energia Atomica, San Martin (Argentina); Consejo Nacional de Investigaciones Cientificas y Tecnicas, Buenos Aires (Argentina); Escuela de Ciencia y Tecnologia, Universidad Nacional de San Martin (Argentina); Burlon, Alejandro A. [Comision Nacional de Energia Atomica, San Martin (Argentina); Escuela de Ciencia y Tecnologia, Universidad Nacional de San Martin (Argentina); Valda, Alejandro [Escuela de Ciencia y Tecnologia, Universidad Nacional de San Martin (Argentina); Navalesi, Daniela [Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires (Argentina); Davidson, Jorge; Davidson, Miguel [Consejo Nacional de Investigaciones Cientificas y Tecnicas, Buenos Aires (Argentina); Vazquez, Monica; Ozafran, Mabel [Comision Nacional de Energia Atomica, San Martin (Argentina); Duran, Hebe [Comision Nacional de Energia Atomica, San Martin (Argentina); Consejo Nacional de Investigaciones Cientificas y Tecnicas, Buenos Aires (Argentina); Escuela de Ciencia y Tecnologia, Universidad Nacional de San Martin (Argentina)], E-mail: hduran@cnea.gov.ar

2009-07-15T23:59:59.000Z

9

The Impact of Individual In Vivo Repair of DNA Double-Strand Breaks on Oral Mucositis in Adjuvant Radiotherapy of Head-and-Neck Cancer  

SciTech Connect (OSTI)

Purpose: To evaluate the impact of individual in vivo DNA double-strand break (DSB) repair capacity on the incidence of severe oral mucositis in patients with head-and-neck cancer undergoing adjuvant radiotherapy (RT) or radiochemotherapy (RCT). Patients and Methods: Thirty-one patients with resected head-and-neck cancer undergoing adjuvant RT or RCT were examined. Patients underwent RT of the primary tumor site and locoregional lymph nodes with a total dose of 60-66 Gy (single dose 2 Gy, five fractions per week). Chemotherapy consisted of two cycles of cisplatin and 5-fluorouracil. To assess DSB repair, {gamma}-H2AX foci in blood lymphocytes were quantified before and 0.5 h, 2.5 h, 5 h, and 24 h after in vivo radiation exposure (the first fraction of RT). World Health Organization scores for oral mucositis were documented weekly and correlated with DSB repair. Results: Sixteen patients received RT alone; 15 patients received RCT. In patients who developed Grade {>=} 3 mucositis (n = 18) the amount of unrepaired DSBs 24 h after radiation exposure and DSB repair half-times did not differ significantly from patients with Grade {<=}2 mucositis (n = 13). Patients with a proportion of unrepaired DSBs after 24 h higher than the mean value + one standard deviation had an increased incidence of severe oral mucositis. Conclusions: Evaluation of in vivo DSB repair by determination of {gamma}-H2AX foci loss is feasible in clinical practice and allows identification of patients with impaired DSB repair. The incidence of oral mucositis is not closely correlated with DSB repair under the evaluated conditions.

Fleckenstein, Jochen, E-mail: rajfle@uks.eu [Department of Radiotherapy and Radiation Oncology, Saarland University Medical School, Homburg (Germany); Kuehne, Martin; Seegmueller, Katharina; Derschang, Sarah; Melchior, Patrick [Department of Radiotherapy and Radiation Oncology, Saarland University Medical School, Homburg (Germany); Graeber, Stefan [Institute for Medical Biometry, Epidemiology und Medical Informatics (IMBEI), Saarland University Medical School, Homburg (Germany); Fricke, Andreas; Ruebe, Claudia E.; Ruebe, Christian [Department of Radiotherapy and Radiation Oncology, Saarland University Medical School, Homburg (Germany)

2011-12-01T23:59:59.000Z

10

Replication protein A and g-H2AX foci assembly is triggered by cellular response to DNA double-strand breaks  

E-Print Network [OSTI]

pathway that removes the oxidized base lesions from the genomic DNA [6]. Evidence for the stimulation

11

Spin transport and spin polarization properties in double-stranded DNA  

SciTech Connect (OSTI)

We study the spin-dependent electron transport through a double-stranded DNA (dsDNA) using the Bogoliubov-de Gennes equations and non-equilibrium Green's function method. We calculate the spin-dependent electron conductance and spin-polarization for different lengths, helix angles, twist angles of dsDNA, the environment-induced dephasing factors, and hopping integral. It is shown that the conductance decreases by increasing the length and dephasing factor. Also, we show that the spin-polarization depends on the helical symmetry and the length of DNA. It is shown that the double-stranded DNA can act as a perfect spin filter. Finally, we show that the sign of spin polarization can be inverted from +1 (?1) to ?1 (+1) for some values of hopping integral.

Simchi, Hamidreza, E-mail: simchi@iust.ac.ir [Department of Physics, Iran University of Science and Technology, Narrmak, Tehran 16844 (Iran, Islamic Republic of); Semiconductor Technology Center, Tehran (Iran, Islamic Republic of); Esmaeilzadeh, Mahdi, E-mail: mahdi@iust.ac.ir; Mazidabadi, Hossein [Department of Physics, Iran University of Science and Technology, Narrmak, Tehran 16844 (Iran, Islamic Republic of)

2013-11-21T23:59:59.000Z

12

auger electron-induced double-strand: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

STUDY OF DNA DOUBLE-STRAND BREAKS IN BYSTANDER PRIMARY HUMAN FIBROBLASTS L. B. Smilenov-or-nothing manner(7) . Bystander cells exhibit a variety of characteristics of...

13

Site-Selective Binding of Nanoparticles to Double-Stranded DNA via Peptide Nucleic Acid "Invasion"  

SciTech Connect (OSTI)

We demonstrate a novel method for by-design placement of nano-objects along double-stranded (ds) DNA. A molecular intercalator, designed as a peptide nucleic acid (PNA)-DNA chimera, is able to invade dsDNA at the PNA-side due to the hybridization specificity between PNA and one of the duplex strands. At the same time, the single-stranded (ss) DNA tail of the chimera, allows for anchoring of nano-objects that have been functionalized with complementary ssDNA. The developed method is applied for interparticle attachment and for the fabrication of particle clusters using a dsDNA template. This method significantly broadens the molecular toolbox for constructing nanoscale systems by including the most conventional not yet utilized DNA motif, double helix DNA.

Stadler, A.L.; van der Lelie, D.; Sun, D.; Maye, M. M.; Gang, O.

2011-04-01T23:59:59.000Z

14

Proximity-induced superconductivity effect in a double-stranded DNA  

SciTech Connect (OSTI)

We study the proximity-induced superconductivity effect in a double-stranded DNA by solving the Bogoliubov-de Gennes equations and taking into account the effect of thermal fluctuations of the twist angle between neighboring base pairs. We show that the electron conductance is spin-dependent and the conductance of spin up (down) increases (decreases) due to the spin-orbit coupling (SOC). It is found that, for T?

Simchi, Hamidreza, E-mail: simchi@iust.ac.ir [Department of Physics, Iran University of Science and Technology, Narrmak, Tehran 16844 (Iran, Islamic Republic of); Semiconductor Technology Center, Tehran (Iran, Islamic Republic of); Esmaeilzadeh, Mahdi, E-mail: mahdi@iust.ac.ir; Mazidabadi, Hossein [Department of Physics, Iran University of Science and Technology, Narrmak, Tehran 16844 (Iran, Islamic Republic of)

2014-02-07T23:59:59.000Z

15

Mechanistic studies of bleomycin-mediated double-stranded DNA cleavage and structural studies of DNA containing normal and 4'-oxidized abasic sites  

E-Print Network [OSTI]

In order to examine the role of partial intercalation in double-stranded (ds) DNA cleavage mediated by a single bleomycin (BLM), a bulky group ([-cyclodextrin) was chemically attached to the polyamine tail of BLM A5 to ...

Chen, Jingyang, Ph. D. Massachusetts Institute of Technology

2006-01-01T23:59:59.000Z

16

Double stranded nucleic acid biochips  

DOE Patents [OSTI]

This invention describes a new method of constructing double-stranded DNA (dsDNA) microarrays based on the use of pre-synthesized or natural DNA duplexes without a stem-loop structure. The complementary oligonucleotide chains are bonded together by a novel connector that includes a linker for immobilization on a matrix. A non-enzymatic method for synthesizing double-stranded nucleic acids with this novel connector enables the construction of inexpensive and robust dsDNA/dsRNA microarrays. DNA-DNA and DNA-protein interactions are investigated using the microarrays.

Chernov, Boris; Golova, Julia

2006-05-23T23:59:59.000Z

17

Intense photoluminescence from dried double-stranded DNA and single-walled carbon nanotube hybrid  

SciTech Connect (OSTI)

Semiconducting single-walled carbon nanotubes (SWNTs) show near-infrared photoluminescence (PL) when they are individually isolated. This was an obstacle to use photonic properties of SWNTs on a solid surface. We show that SWNTs wrapped with DNA maintain intense PL under the dry conditions. SWNTs are well isolated individually by DNA even when the DNA-SWNT hybrids are agglomerated. This finding opens up application of SWNTs to photonic devices.

Ito, M.; Kobayashi, T.; Ito, Y.; Hayashida, T.; Nii, D.; Umemura, K.; Homma, Y. [Department of Physics, Tokyo University of Science, Tokyo 162-8601 (Japan)

2014-01-27T23:59:59.000Z

18

Dyes designed for high sensitivity detection of double-stranded DNA  

DOE Patents [OSTI]

Novel fluorescent dyes are provided, characterized by having a fluorophore joined to a cationic chain. The dyes are found to provide for high enhancement upon binding to nucleic acid and have strong binding affinities to the nucleic acid, as compared to the fluorophore without the polycationic chain. The dyes find use in detection of dsDNA in gel electrophoresis and solution at substantially higher sensitivities using substantially less dye.

Glazer, Alexander N. (Orinda, CA); Benson, Scott C. (Oakland, CA)

1998-01-01T23:59:59.000Z

19

Dyes designed for high sensitivity detection of double-stranded DNA  

DOE Patents [OSTI]

Novel fluorescent dyes are provided, characterized by having a fluorophore joined to a polycationic chain of at least two positive charges. The dyes are found to provide for high enhancement upon binding to nucleic acid and have strong binding affinities to the nucleic acid, as compared to the fluorophore without the polycationic chain. The dyes find use in detection of dsDNA in gel electrophoresis and solution at substantially higher sensitivities using substantially less dye.

Glazer, Alexander N. (Orinda, CA); Benson, Scott C. (Albany, CA)

1994-01-01T23:59:59.000Z

20

Dyes designed for high sensitivity detection of double-stranded DNA  

DOE Patents [OSTI]

Novel fluorescent dyes are provided, characterized by having a fluorophore joined to a cationic chain. The dyes are found to provide for high enhancement upon binding to nucleic acid and have strong binding affinities to the nucleic acid, as compared to the fluorophore without the polycationic chain. The dyes find use in detection of dsDNA in gel electrophoresis and solution at substantially higher sensitivities using substantially less dye.

Glazer, Alexander N. (Orinda, CA); Benson, Scott C. (Oakland, CA)

2000-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "dna double-strand breaks" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


21

ancient human dna: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

STUDY OF DNA DOUBLE-STRAND BREAKS IN BYSTANDER PRIMARY HUMAN FIBROBLASTS L. B. Smilenov-or-nothing manner(7) . Bystander cells exhibit a variety of characteristics of...

22

ancient dna studies: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

STUDY OF DNA DOUBLE-STRAND BREAKS IN BYSTANDER PRIMARY HUMAN FIBROBLASTS L. B. Smilenov-or-nothing manner(7) . Bystander cells exhibit a variety of characteristics of...

23

Investigating Deinococcus radiodurans RecA Protein Filament Formation on Double-Stranded DNA by a Real-Time  

E-Print Network [OSTI]

to desiccation.2 Many hypotheses have been proposed to explain the high- efficiency repair, including a ringlike condensed chromosome structure that could restrict fragment DNA diffusion,3-5 a high Mn2+ concentration: With the aid of an efficient, precise, and almost error-free DNA repair system, Deinococcus radiodurans can

Cox, Michael M.

24

ORIGINAL PAPER Ionizing radiation induces DNA double-strand breaks in bystander primary  

E-Print Network [OSTI]

fibroblasts Mykyta V Sokolov1,2 , Lubomir B Smilenov3 , Eric J Hall3 , Igor G Panyutin1 , William M Bonner*,4

25

Induction of linear tracks of DNA double-strand breaks by -particle irradiation of  

E-Print Network [OSTI]

eukaryotic cells by exposing the cells to particles from a small Americium source (Box 1). Each particle) EQUIPMENT Americium (Am-241) source (SupplementaryMethods and SupplementaryFigs.1­3 online) Polyester film

Cai, Long

26

A MICROBEAM STUDY OF DNA DOUBLE-STRAND BREAKS IN BYSTANDER PRIMARY HUMAN FIBROBLASTS  

E-Print Network [OSTI]

-or-nothing manner(7) . Bystander cells exhibit a variety of characteristics of IR-induced genomic instability

27

DNA purification by triplex-affinity capture and affinity capture electrophoresis  

DOE Patents [OSTI]

The invention provides a method for purifying or isolating double stranded DNA intact using triple helix formation. The method includes the steps of complexing an oligonucleotide and double stranded DNA to generate a triple helix and immobilization of the triple helix on a solid phase by means of a molecular recognition system such as avidin/biotin. The purified DNA is then recovered intact by treating the solid phase with a reagent that breaks the bonds between the oligonucleotide and the intact double stranded DNA while not affecting the Watson-Crick base pairs of the double helix. The present invention also provides a method for purifying or isolating double stranded DNA intact by complexing the double stranded DNA with a specific binding partner and recovering the complex during electrophoresis by immobilizing it on a solid phase trap imbedded in an electrophoretic gel. 6 figs.

Cantor, C.R.; Ito, Takashi; Smith, C.L.

1996-01-09T23:59:59.000Z

28

Identification, Exploitation and Manipulation of BRCA1-Dependent DNA Double-Strand Break and Interstrand Crosslink Repair in Breast and Ovarian Cancer Therapy  

E-Print Network [OSTI]

Expression of functional breast cancer susceptibility gene 1 (BRCA1) in human cancers is associated with resistance to platinum-based chemotherapeutics and poly(ADP-ribose) polymerase (PARP) inhibitors. BRCA1 is a nuclear ...

Stecklein, Shane Richard

2012-05-12T23:59:59.000Z

29

Polymerization dynamics of double-stranded biopolymers: Chemical kinetic approach  

E-Print Network [OSTI]

Polymerization dynamics of double-stranded biopolymers: Chemical kinetic approach Evgeny B 19 November 2004; accepted 20 December 2004; published online 10 March 2005 The polymerization by the processes that take place during their polymerization. However, our understanding of the coupling between

30

Asymmetric quantum transport in a double-stranded Kronig-Penney model  

E-Print Network [OSTI]

We introduce a double-stranded Kronig-Penney model and analyze its transport properties. The asymmetric fluxes between two strands with suddenly alternating localization patterns are found as the energy is varied. The zero-size limit of the internal lines connecting two strands is examined using quantum graph vertices with four edges. We also consider a two-dimensional Kronig-Penney lattice with two types of alternating layers with $\\delta$ and $\\delta'$ connections, and show that the existence of energy bands in which the quantum flux can flow only in selected directions.

Taksu Cheon; Sergey S. Poghosyan

2015-03-03T23:59:59.000Z

31

Exotic quantum transport in double-stranded Kronig-Penney model  

E-Print Network [OSTI]

We introduce double-stranded Kronig-Penney model and analyze its transport properties. The asymmetric fluxes between two strands with suddenly alternating localization patterns are found as the energy is varied. The zero-size limit of the internal lines connecting two strands is examined using quantum graph vertices with four edges. We also consider a two-dimensional Kronig-Penney lattice with two types of alternating layers with $\\delta$ and $\\delta'$ connections, and show that the existence of energy bands in which the quantum flux can flow only in selected directions.

Taksu Cheon; Sergey S. Poghosyan

2014-10-31T23:59:59.000Z

32

DNA Repair Alterations in Children With Pediatric Malignancies: Novel Opportunities to Identify Patients at Risk for High-Grade Toxicities  

SciTech Connect (OSTI)

Purpose: To evaluate, in a pilot study, the phosphorylated H2AX ({gamma}H2AX) foci approach for identifying patients with double-strand break (DSB) repair deficiencies, who may overreact to DNA-damaging cancer therapy. Methods and Materials: The DSB repair capacity of children with solid cancers was analyzed compared with that of age-matched control children and correlated with treatment-related normal-tissue responses (n = 47). Double-strand break repair was investigated by counting {gamma}H2AX foci in blood lymphocytes at defined time points after irradiation of blood samples. Results: Whereas all healthy control children exhibited proficient DSB repair, 3 children with tumors revealed clearly impaired DSB repair capacities, and 2 of these repair-deficient children developed life-threatening or even lethal normal-tissue toxicities. The underlying mutations affecting regulatory factors involved in DNA repair pathways were identified. Moreover, significant differences in mean DSB repair capacity were observed between children with tumors and control children, suggesting that childhood cancer is based on genetic alterations affecting DSB repair function. Conclusions: Double-strand break repair alteration in children may predispose to cancer formation and may affect children's susceptibility to normal-tissue toxicities. Phosphorylated H2AX analysis of blood samples allows one to detect DSB repair deficiencies and thus enables identification of children at risk for high-grade toxicities.

Ruebe, Claudia E., E-mail: claudia.ruebe@uks.e [Department of Radiation Oncology, Saarland University, Homburg/Saar (Germany); Fricke, Andreas; Schneider, Ruth; Simon, Karin; Kuehne, Martin; Fleckenstein, Jochen [Department of Radiation Oncology, Saarland University, Homburg/Saar (Germany); Graeber, Stefan [Institute of Medical Biometrics, Epidemiology and Medical Informatics, Saarland University, Homburg/Saar (Germany); Graf, Norbert [Department of Pediatric Hematology and Oncology, Saarland University, Homburg/Saar (Germany); Ruebe, Christian [Department of Radiation Oncology, Saarland University, Homburg/Saar (Germany)

2010-10-01T23:59:59.000Z

33

Screening Tool for Providers of Double-Stranded DNA - Energy Innovation  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmosphericNuclear Security Administrationcontroller systemsBi (2) Sr (2)ScienceScientists In thePrincetonScottPortal

34

Mitigating security issues in the evolving DNA synthesis industry  

E-Print Network [OSTI]

DNA synthesis technologies are advancing at exponential rates, with production of ever longer, more complex, and less expensive sequences of double stranded DNA. This has fostered development of industrial scale design, ...

Turlington, Ralph Donald, III

2013-01-01T23:59:59.000Z

35

aluminum strand coating: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

STUDY OF DNA DOUBLE-STRAND BREAKS IN BYSTANDER PRIMARY HUMAN FIBROBLASTS L. B. Smilenov by assessing DNA double-strand break (DSB) formation in situ with the rapid and...

36

Activation of double-stranded RNA-dependent protein kinase inhibits proliferation of pancreatic ?-cells  

SciTech Connect (OSTI)

Highlights: •PKR can be activated by glucolipitoxicity and pro-inflammatory cytokines in ?-cells. •Activated PKR inhibited ?-cell proliferation by arresting cell cycle at G1 phase. •Activated PKR fully abrogated the pro-proliferative effects of IGF-I on ?-cells. -- Abstract: Double-stranded RNA-dependent protein kinase (PKR) is revealed to participate in the development of insulin resistance in peripheral tissues in type 2 diabetes (T2DM). Meanwhile, PKR is also characterized as a critical regulator of cell proliferation. To date, no study has focused on the impact of PKR on the proliferation of pancreatic ?-cells. Here, we adopted insulinoma cell lines and mice islet ?-cells to investigate: (1) the effects of glucolipotoxicity and pro-inflammatory cytokines on PKR activation; (2) the effects of PKR on proliferation of pancreatic ?-cells and its underlying mechanisms; (3) the actions of PKR on pro-proliferative effects of IGF-I and its underlying pathway. Our results provided the first evidence that PKR can be activated by glucolipitoxicity and pro-inflammatory cytokines in pancreatic ?-cells, and activated PKR significantly inhibited cell proliferation by arresting cell cycle at G1 phase. Reductions in cyclin D1 and D2 as well as increases in p27 and p53 were associated with the anti-proliferative effects of PKR, and proteasome-dependent degradation took part in the reduction of cyclin D1 and D2. Besides, PKR activation abrogated the pro-proliferative effects of IGF-I by activating JNK and disrupting IRS1/PI3K/Akt signaling pathway. These findings indicate that the anti-proliferative actions of PKR on pancreatic ?-cells may contribute to the pathogenesis of T2DM.

Chen, Shan-Shan [Key Laboratory of Human Functional Genomics of Jiangsu Province, Nanjing Medical University, Nanjing (China) [Key Laboratory of Human Functional Genomics of Jiangsu Province, Nanjing Medical University, Nanjing (China); Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing (China); Jiang, Teng [Department of Neurology, Qingdao Municipal Hospital, Nanjing Medical University, Nanjing (China)] [Department of Neurology, Qingdao Municipal Hospital, Nanjing Medical University, Nanjing (China); Wang, Yi; Gu, Li-Ze [Key Laboratory of Human Functional Genomics of Jiangsu Province, Nanjing Medical University, Nanjing (China) [Key Laboratory of Human Functional Genomics of Jiangsu Province, Nanjing Medical University, Nanjing (China); Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing (China); Wu, Hui-Wen [Laboratory Center for Basic Medical Sciences, Nanjing Medical University, Nanjing (China)] [Laboratory Center for Basic Medical Sciences, Nanjing Medical University, Nanjing (China); Tan, Lan [Department of Neurology, Qingdao Municipal Hospital, Nanjing Medical University, Nanjing (China)] [Department of Neurology, Qingdao Municipal Hospital, Nanjing Medical University, Nanjing (China); Guo, Jun, E-mail: Guoj@njmu.edu.cn [Key Laboratory of Human Functional Genomics of Jiangsu Province, Nanjing Medical University, Nanjing (China) [Key Laboratory of Human Functional Genomics of Jiangsu Province, Nanjing Medical University, Nanjing (China); Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing (China)

2014-01-17T23:59:59.000Z

37

Mutations Abrogating VP35 Interaction with Double-Stranded RNA Render Ebola Virus Avirulent in Guinea Pigs  

SciTech Connect (OSTI)

Ebola virus (EBOV) protein VP35 is a double-stranded RNA (dsRNA) binding inhibitor of host interferon (IFN)-{alpha}/{beta} responses that also functions as a viral polymerase cofactor. Recent structural studies identified key features, including a central basic patch, required for VP35 dsRNA binding activity. To address the functional significance of these VP35 structural features for EBOV replication and pathogenesis, two point mutations, K319A/R322A, that abrogate VP35 dsRNA binding activity and severely impair its suppression of IFN-{alpha}/{beta} production were identified. Solution nuclear magnetic resonance (NMR) spectroscopy and X-ray crystallography reveal minimal structural perturbations in the K319A/R322A VP35 double mutant and suggest that loss of basic charge leads to altered function. Recombinant EBOVs encoding the mutant VP35 exhibit, relative to wild-type VP35 viruses, minimal growth attenuation in IFN-defective Vero cells but severe impairment in IFN-competent cells. In guinea pigs, the VP35 mutant virus revealed a complete loss of virulence. Strikingly, the VP35 mutant virus effectively immunized animals against subsequent wild-type EBOV challenge. These in vivo studies, using recombinant EBOV viruses, combined with the accompanying biochemical and structural analyses directly correlate VP35 dsRNA binding and IFN inhibition functions with viral pathogenesis. Moreover, these studies provide a framework for the development of antivirals targeting this critical EBOV virulence factor.

Prins, Kathleen C.; Delpeut, Sebastien; Leung, Daisy W.; Reynard, Olivier; Volchkova, Valentina A.; Reid, St. Patrick; Ramanan, Parameshwaran; Cárdenas, Washington B.; Amarasinghe, Gaya K.; Volchkov, Viktor E.; Basler, Christopher F. (CNRS-INSERM); (Mount Sinai Hospital); (LB-Ecuador); (Iowa State)

2010-10-11T23:59:59.000Z

38

Double-strand DNA-templated formation of copper nanoparticles as fluorescent probe for label free nuclease enzyme detection  

E-Print Network [OSTI]

cleavage activity. Thus, a label-free strategy for sensitive detection of nuclease has been developed B.V. All rights reserved. 1. Introduction As promising substitutes for organic dyes and quantum dots epithelial cervical cancer cells (HeLa cells) using fluorescent gold nanoparticles which emitted a stable

Tan, Weihong

39

ExpandplusCrystal Structures of Poly(ADP-ribose) Polymerase-1 (PARP-1) Zinc Fingers Bound to DNA  

SciTech Connect (OSTI)

Poly(ADP-ribose) polymerase-1 (PARP-1) has two homologous zinc finger domains, Zn1 and Zn2, that bind to a variety of DNA structures to stimulate poly(ADP-ribose) synthesis activity and to mediate PARP-1 interaction with chromatin. The structural basis for interaction with DNA is unknown, which limits our understanding of PARP-1 regulation and involvement in DNA repair and transcription. Here, we have determined crystal structures for the individual Zn1 and Zn2 domains in complex with a DNA double strand break, providing the first views of PARP-1 zinc fingers bound to DNA. The Zn1-DNA and Zn2-DNA structures establish a novel, bipartite mode of sequence-independent DNA interaction that engages a continuous region of the phosphodiester backbone and the hydrophobic faces of exposed nucleotide bases. Biochemical and cell biological analysis indicate that the Zn1 and Zn2 domains perform distinct functions. The Zn2 domain exhibits high binding affinity to DNA compared with the Zn1 domain. However, the Zn1 domain is essential for DNA-dependent PARP-1 activity in vitro and in vivo, whereas the Zn2 domain is not strictly required. Structural differences between the Zn1-DNA and Zn2-DNA complexes, combined with mutational and structural analysis, indicate that a specialized region of the Zn1 domain is re-configured through the hydrophobic interaction with exposed nucleotide bases to initiate PARP-1 activation.

M Langelier; J Planck; S Roy; J Pascal

2011-12-31T23:59:59.000Z

40

ageing human fibroblasts: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

STUDY OF DNA DOUBLE-STRAND BREAKS IN BYSTANDER PRIMARY HUMAN FIBROBLASTS L. B. Smilenov-or-nothing manner(7) . Bystander cells exhibit a variety of characteristics of...

Note: This page contains sample records for the topic "dna double-strand breaks" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


41

ataxia telangiectasia fibroblasts: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

STUDY OF DNA DOUBLE-STRAND BREAKS IN BYSTANDER PRIMARY HUMAN FIBROBLASTS L. B. Smilenov-or-nothing manner(7) . Bystander cells exhibit a variety of characteristics of...

42

acidic fibroblast growth: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

STUDY OF DNA DOUBLE-STRAND BREAKS IN BYSTANDER PRIMARY HUMAN FIBROBLASTS L. B. Smilenov-or-nothing manner(7) . Bystander cells exhibit a variety of characteristics of...

43

adult-onset primary open-angle: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

STUDY OF DNA DOUBLE-STRAND BREAKS IN BYSTANDER PRIMARY HUMAN FIBROBLASTS L. B. Smilenov-or-nothing manner(7) . Bystander cells exhibit a variety of characteristics of...

44

angiogenic gene-modified fibroblasts: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

STUDY OF DNA DOUBLE-STRAND BREAKS IN BYSTANDER PRIMARY HUMAN FIBROBLASTS L. B. Smilenov-or-nothing manner(7) . Bystander cells exhibit a variety of characteristics of...

45

acid-induced fibroblast cell: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

STUDY OF DNA DOUBLE-STRAND BREAKS IN BYSTANDER PRIMARY HUMAN FIBROBLASTS L. B. Smilenov-or-nothing manner(7) . Bystander cells exhibit a variety of characteristics of...

46

autologous fibroblast metabolism: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

STUDY OF DNA DOUBLE-STRAND BREAKS IN BYSTANDER PRIMARY HUMAN FIBROBLASTS L. B. Smilenov-or-nothing manner(7) . Bystander cells exhibit a variety of characteristics of...

47

acid induces human: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

STUDY OF DNA DOUBLE-STRAND BREAKS IN BYSTANDER PRIMARY HUMAN FIBROBLASTS L. B. Smilenov-or-nothing manner(7) . Bystander cells exhibit a variety of characteristics of...

48

arthritis synovial fibroblasts: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

STUDY OF DNA DOUBLE-STRAND BREAKS IN BYSTANDER PRIMARY HUMAN FIBROBLASTS L. B. Smilenov-or-nothing manner(7) . Bystander cells exhibit a variety of characteristics of...

49

ATP Hydrolysis and DNA Binding by the Escherichia coli RecF Protein*  

E-Print Network [OSTI]

ATP Hydrolysis and DNA Binding by the Escherichia coli RecF Protein* (Received for publication ATP hydrolytic activity. ATP hydrolysis leads to RecF dissociation from double-stranded (ds to DNA. A mutant RecF protein that can bind but cannot hydrolyze ATP (RecF K36R) does not readily

Cox, Michael M.

50

Derivatized versions of ligase enzymes for constructing DNA sequences  

DOE Patents [OSTI]

A method of making very long, double-stranded synthetic poly-nucleotides. A multiplicity of short oligonucleotides is provided. The short oligonucleotides are sequentially hybridized to each other. Enzymatic ligation of the oligonucleotides provides a contiguous piece of PCR-ready DNA of predetermined sequence.

Mariella, Jr., Raymond P. (Danville, CA); Christian, Allen T. (Tracy, CA); Tucker, James D. (Novi, MN); Dzenitis, John M. (Livermore, CA); Papavasiliou, Alexandros P. (Oakland, CA)

2006-08-15T23:59:59.000Z

51

DUPLEX: A molecular mechanics program in torsion angle space for computing structures of DNA and RNA  

SciTech Connect (OSTI)

DUPLEX produces energy minimized structures of DNA and RNA of any base sequence for single and double strands. The smallest subunits are deoxydinucleoside monophosphates, and up to 12 residues, single or double stranded can be treated. In addition, it can incorporate NMR derived interproton distances an constraints in the minimizations. Both upper and lower bounds for these distances can be specified. The program has been designed to run on a UNICOS Cray supercomputer, but should run, albeit slowly, on a laboratory computer such as a VAX or a workstation.

Hingerty, B.E.

1992-07-01T23:59:59.000Z

52

Collection, focusing, and metering of DNA in microchannels using addressable electrode arrays for portable low-power bioanalysis  

E-Print Network [OSTI]

the funding for this work. vii NOMENCLATURE DNA Deoxyribonucleic Acid dsDNA Double Stranded DNA ssDNA Single Stranded DNA EDTA Ethylene Diamine Tetracetic Acid TBE Tris-Borate EDTA BME Beta Mercapto Ethanol PCB Printed Circuit Board DI Deionized... through a USB cable using a Personal Computer........................................................................................ 66 Figure 27 Top level block diagram of the FPGA based portable analysis device showing flow of information...

Shaikh, Faisal

2008-10-10T23:59:59.000Z

53

Electrophoretic detection and separation of mutant DNA using replaceable polymer matrices  

DOE Patents [OSTI]

The disclosure relates to a method for resolving double-stranded DNA species differing by at least one base pair. Each of the species is characterized by an iso-melting domain with a unique melting temperature contiguous with a melting domain of higher thermal stability. 18 figs.

Karger, B.L.; Thilly, W.G.; Foret, F.; Khrapko, K.; Koehavong, P.; Cohen, A.S.; Giese, R.W.

1997-05-27T23:59:59.000Z

54

Divalent Ion and Thermally Induced DNA Conformational Polymorphism on Single-walled  

E-Print Network [OSTI]

coverage on nanotubes dispersed in aqueous solution decreases, the emission energy decreases (due Nanotubes Hong Jin1 , Esther S. Jeng1 , Daniel A. Heller2 , Prakrit V. Jena3 , Robert Kirmse4 , Jörg and double stranded DNA can wrap and colloidally stabilize single walled carbon nanotubes in solution

Langowski, Jörg

55

Electrophoretic detection and separation of mutant DNA using replaceable polymer matrices  

DOE Patents [OSTI]

The disclosure relates to a method for resolving double-stranded DNA species differing by at least one base pair. Each of the species is characterized by an iso-melting domain with a unique melting temperature contiguous with a melting domain of higher thermal stability.

Karger, Barry L. (Newton, MA); Thilly, William G. (Winchester, MA); Foret, Frantisek (Malden, MA); Khrapko, Konstaintin (Brookline, MA); Koehavong, Phouthone (Pittsburgh, PA); Cohen, Aharon S. (Newton, MA); Giese, Roger W. (Quincy, MA)

1997-01-01T23:59:59.000Z

56

Induction and Persistence of Large ?H2AX Foci by High Linear Energy Transfer Radiation in DNA-Dependent protein kinase–Deficient Cells  

SciTech Connect (OSTI)

Purpose: To evaluate the cell response to DNA double-strand breaks induced by low and high linear energy transfer (LET) radiations when the catalytic subunit of DNA-dependent protein kinase (DNA-PKcs), an essential protein of the nonhomologous end-joining repair pathway, lacks kinase activity. Methods and Materials: CHO10B2, a Chinese hamster ovary cell line, and its derived radiosensitive mutant cell line, irs-20, lacking DNA-PKcs activity, were evaluated after 0 to 3 Gy of ?-rays, plateau and Bragg peak protons, and lithium beams by clonogenic assay, and as a measurement of double-strand breaks, phosphorylated H2AX (?H2AX) foci number and size were quantified by immunocytofluorescence. Results: Irs-20 exhibited greater radiosensitivity and a higher amount of ?H2AX foci than CHO10B2 at 6 hours after irradiation for all types of radiations. Remarkably, CHO10B2 and irs-20 maintained their difference in radiosensitivity after high-LET radiation. Six hours after low-LET radiations, irs-20 did not reach basal levels of ?H2AX at high doses, whereas CHO10B2 recovered basal levels for all doses. After high-LET radiation, only CHO10B2 exhibited a reduction in ?H2AX foci, but it never reached basal levels. Persistent foci in irs-20 confirmed a repair deficiency. Interestingly, after 30 minutes of high-LET radiation both cell lines exhibited large foci (size >0.9 ?m{sup 2}) related to the damage nature, whereas at 6 hours irs-20 showed a higher amount of large foci than CHO10B2, with a 7-fold increase at 3 Gy, that could also be associated to radiosensitivity. Conclusions: We demonstrated, for the first time, an association between deficient DNA-PKcs activity and not only high levels of H2AX phosphorylation but also persistence and size increase of ?H2AX foci after high-LET irradiation.

Bracalente, Candelaria; Ibañez, Irene L. [Departamento de Micro y Nanotecnología, Comisión Nacional de Energía Atómica, San Martín, Buenos Aires (Argentina); Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires (Argentina); Molinari, Beatriz [Departamento de Radiobiología, Comisión Nacional de Energía Atómica, San Martín, Buenos Aires (Argentina); Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires (Argentina); Palmieri, Mónica [Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires (Argentina); Kreiner, Andrés [Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires (Argentina); Gerencia de Investigación y Aplicaciones, Comisión Nacional de Energía Atómica, San Martín, Buenos Aires (Argentina); Escuela de Ciencia y Tecnología, Universidad Nacional de San Martín, San Martín, Buenos Aires (Argentina); Valda, Alejandro [Escuela de Ciencia y Tecnología, Universidad Nacional de San Martín, San Martín, Buenos Aires (Argentina); and others

2013-11-15T23:59:59.000Z

57

Analysis of cell cycle surveillance mechanisms in meiosis  

E-Print Network [OSTI]

Numerous DNA double-strand breaks (DSBs) are introduced into the genome in the course of meiotic recombination. This poses a significant hazard to the genomic integrity of the cell. Studies in a number of organisms have ...

Hochwagen, Andreas

2006-01-01T23:59:59.000Z

58

The impact of age, exposure and genetics on homologous recombination at the engineered repeat sequence in mice  

E-Print Network [OSTI]

Mitotic homologous recombination is a critical pathway for the repair of DNA double-strand breaks and broken replication forks. Although homologous recombination is generally error-free, recombination between misaligned ...

Wiktor-Brown, Dominika M

2007-01-01T23:59:59.000Z

59

Rapid purification of circular DNA by triplex-mediated affinity capture  

DOE Patents [OSTI]

A single-step capture of a target supercoiled double-stranded DNA molecule is accomplished by forming a local triple-helix among two strands of the supercoiled circular DNA and an oligonucleotide probe. The oligonucleotide is bound to an immobilizing support which facilitates the immobilization and purification of target DNA molecules. Non-target DNA molecules and other contaminating cellular material are easily removed by washing. The triple-helical structure is destabilized by raising the pH, leaving purified target DNA in the supernatant and reusable affinity capture oligonucleotide secured to the immobilizing support. 3 figs.

Ji, H.; Smith, L.M.

1997-01-07T23:59:59.000Z

60

Mass spectrometric characterization of sequence-specific complexes of DNA and transcription factor PU.1 DNA binding domain  

SciTech Connect (OSTI)

Electrospray ionization mass spectrometry (ESI-MS) has been used to study the noncovalent interaction of the 13.5-kDa DNA binding domain of PU.1 (PU.1-DBD) with specific double-stranded DNA (dsDNA) target molecules. Mixtures of PU.1-DBD protein and wildtype target DNA sequence yielded ESI-MS spectra showing only protein-dsDNA complex ions of 1:1 stoichiometry and free dsDNA. When PU.1-DBD protein, wild type target DNA, and a mutant target DNA lacking the consensus sequence were mixed, only the 1:1 complex with the wild-type DNA was observed, consistent with gel electrophoresis mobility shift assay results, demonstrating the observation of sequence-specific protein-dsDNA complexes using ESI-MS. 22 refs., 5 figs., 1 tab.

Cheng, Xueheng; Harms, A.C.; Bruce, J.E. [Pacific Northwest National Lab., Richland, WA (United States)] [and others] [Pacific Northwest National Lab., Richland, WA (United States); and others

1996-07-15T23:59:59.000Z

Note: This page contains sample records for the topic "dna double-strand breaks" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


61

Slow elimination of phosphorylated histone {gamma}-H2AX from DNA of terminally differentiated mouse heart cells in situ  

SciTech Connect (OSTI)

Phosphorylation of replacement histone H2AX occurs in megabase chromatin domains around double-strand DNA breaks (DSBs) and this modification (called {gamma}-H2AX) may serve as a useful marker of genome damage and repair in terminally differentiated cells. Here using immunohistochemistry we studied kinetics of {gamma}-H2AX formation and elimination in the X-irradiated mouse heart and renal epithelial tissues in situ. Unirradiated tissues have 3-5% {gamma}-H2AX-positive cells and in tissues fixed 1 h after X-irradiation {gamma}-H2AX-positive nuclei are induced in a dose-dependent manner approaching 20-30% after 3 Gy of IR. Analysis of mouse tissues at different times after 3 Gy of IR showed that maximal induction of {gamma}-H2AX in heart is observed 20 min after IR and then is decreased slowly with about half remaining 23 h later. In renal epithelium maximum of the {gamma}-H2AX-positive cells is observed 40 min after IR and then decreases to control values in 23 h. This indicates that there are significant variations between non-proliferating mammalian tissues in the initial H2AX phosphorylation rate as well as in the rate of {gamma}-H2AX elimination after X-irradiation, which should be taken into account in the analysis of radiation responses.

Gavrilov, Boris [Institute of Cytology, Russian Academy of Sciences, 194064 St. Petersburg (Russian Federation); Vezhenkova, Irina [Institute of Cytology, Russian Academy of Sciences, 194064 St. Petersburg (Russian Federation); Firsanov, Denis [Institute of Cytology, Russian Academy of Sciences, 194064 St. Petersburg (Russian Federation); Solovjeva, Liudmila [Institute of Cytology, Russian Academy of Sciences, 194064 St. Petersburg (Russian Federation); Svetlova, Maria [Institute of Cytology, Russian Academy of Sciences, 194064 St. Petersburg (Russian Federation); Mikhailov, Vyacheslav [Institute of Cytology, Russian Academy of Sciences, 194064 St. Petersburg (Russian Federation); Tomilin, Nikolai [Institute of Cytology, Russian Academy of Sciences, 194064 St. Petersburg (Russian Federation)]. E-mail: nvtom@hotmail.com

2006-09-08T23:59:59.000Z

62

Structure of gramicidin D-RbCl complex at atomic resolution from low-temperature synchrotron data: interactions of double-stranded gramicidin channel contents and cations with channel wall  

SciTech Connect (OSTI)

Gramicidin D (gD) is a naturally occurring ionophoric antibiotic that forms membrane channels specific for monovalent cations. The crystal structure of the RbCl complex of gD has been determined at 1.14 {angstrom} resolution from low-temperature (100 K) synchrotron-radiation data with a final R of 16%. The structure was refined with anisotropic temperature factors for all non-H atoms and with partial occupancies for many of them. The asymmetric unit in the crystal contains four crystallographically independent molecules that form two right-handed antiparallel double-stranded dimers. There are seven distinct rubidium-binding sites in each dimeric channel. The occupancy factors of Rb cations are between 0.11 and 0.35 and the total ion contents of the two crystallographically independent channels are 1.59 and 1.22 ions, respectively. Although each channel is 'chemically symmetrical', the side-chain conformations, the distributions of rubidium cations and their binding sites in the two independent channels are not. Cations are 'coordinated' by delocalized {pi}-electrons of three to five carbonyl groups that together with peptide backbone chains form the gramicidin channel walls. The water:cation ratio in the channel interior is four or five:one, and five or six waters separate Rb cations during their passage through the channel.

Glówka, M.L.; Olczak, A.; Bojarska, J.; Szczesio, M.; Duax, W.L.; Burkhart, B.M.; Pangborn, W.A.; Langs, D.A.; Wawrzak, Z. (Poland); (NWU); (Hauptman)

2010-03-05T23:59:59.000Z

63

Efficient and simpler method to construct normalized cDNA libraries with improved representations of full-length cDNAs  

DOE Patents [OSTI]

This invention provides a method to normalize a cDNA library comprising: (a) constructing a directionally cloned library containing cDNA inserts wherein the insert is capable of being amplified by polymerase chain reaction; (b) converting a double-stranded cDNA library into single-stranded DNA circles; (c) generating single-stranded nucleic acid molecules complementary to the single-stranded DNA circles converted in step (b) by polymerase chain reaction with appropriate primers; (d) hybridizing the single-stranded DNA circles converted in step (b) with the complementary single-stranded nucleic acid molecules generated in step (c) to produce partial duplexes to an appropriate Cot; and (e) separating the unhybridized single-stranded DNA circles from the hybridized DNA circles, thereby generating a normalized cDNA library. This invention also provides a method to normalize a cDNA library wherein the generating of single-stranded nucleic acid molecules complementary to the single-stranded DNA circles converted in step (b) is by excising cDNA inserts from the double-stranded cDNA library; purifying the cDNA inserts from cloning vectors; and digesting the cDNA inserts with an exonuclease. This invention further provides a method to construct a subtractive cDNA library following the steps described above. This invention further provides normalized and/or subtractive cDNA libraries generated by the above methods. 25 figs.

Soares, M.B.; Fatima Bonaldo, M. de

1998-12-08T23:59:59.000Z

64

Efficient and simpler method to construct normalized cDNA libraries with improved representations of full-length cDNAs  

DOE Patents [OSTI]

This invention provides a method to normalize a cDNA library comprising: (a) constructing a directionally cloned library containing cDNA inserts wherein the insert is capable of being amplified by polymerase chain reaction; (b) converting a double-stranded cDNA library into single-stranded DNA circles; (c) generating single-stranded nucleic acid molecules complementary to the single-stranded DNA circles converted in step (b) by polymerase chain reaction with appropriate primers; (d) hybridizing the single-stranded DNA circles converted in step (b) with the complementary single-stranded nucleic acid molecules generated in step (c) to produce partial duplexes to an appropriate Cot; and (e) separating the unhybridized single-stranded DNA circles from the hybridized DNA circles, thereby generating a normalized cDNA library. This invention also provides a method to normalize a cDNA library wherein the generating of single-stranded nucleic acid molecules complementary to the single-stranded DNA circles converted in step (b) is by excising cDNA inserts from the double-stranded cDNA library; purifying the cDNA inserts from cloning vectors; and digesting the cDNA inserts with an exonuclease. This invention further provides a method to construct a subtractive cDNA library following the steps described above. This invention further provides normalized and/or subtractive cDNA libraries generated by the above methods.

Soares, Marcelo Bento (New York, NY); Bonaldo, Maria de Fatima (New York, NY)

1998-01-01T23:59:59.000Z

65

RADIATION SENSITIVITY & PROCESSING OF DNA DAMAGE FOLLOWING LOW DOSES OF GAMMA-RAY ALPHA PARTICLES & HZE IRRADIATION OF NORMAL DSB REPAIR DEFICIENT CELLS  

SciTech Connect (OSTI)

Non-homologous end joining (NHEJ) predominates in the repair of DNA double strand breaks (DSB) over homologous recombination (HR). NHEJ occurs throughout the cell cycle whereas HR occurs in late S/G2 due to the requirement of a sister chromatid (Rothkamm et al, Mol Cell Biol 23 5706-15 [2003]). To date evidence obtained with DSB repair deficient cells using pulsed-field gel electrophoresis has revealed the major pathway throughout all phases of the cell cycle for processing high dose induced DSBs is NHEJ (Wang et al, Oncogene 20 2212-24 (2001); Pluth et al, Cancer Res. 61 2649-55 [2001]). These findings however were obtained at high doses when on average >> 20-30 DSBs are formed per cell. The contribution of the repair pathways (NHEJ and HR) induced in response to DNA damage during the various phases of the cell cycle may depend upon the dose (the level of initial DSBs) especially since low levels of DSBs are induced at low dose. To date, low dose studies using NHEJ and HR deficient mutants have not been carried out to address this important question with radiations of different quality. The work presented here leads us to suggest that HR plays a relatively minor role in the repair of radiation-induced prompt DSBs. SSBs lead to the induction of DSBs which are associated specifically with S-phase cells consistent with the idea that they are formed at stalled replication forks in which HR plays a major role in repair. That DNA-PKcs is in some way involved in the repair of the precursors to replication-induced DSB remains an open question. Persistent non-DSB oxidative damage also leads to an increase in RAD51 positive DSBs. Both simple and complex non-DSB DNA damage may therefore contribute to indirect DSBs induced by ionising radiation at replication forks.

O'Neil, Peter

2009-05-15T23:59:59.000Z

66

DNA  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

September 15, 2014 Computational analysis key to structural understanding of molecular machine that targets viral DNA LOS ALAMOS, N.M., Sept. 15, 2014-When this week's print issue...

67

GENETIC AND MOLECULAR ANALYSIS OF DNA DAMAGE REPAIR AND TOLERANCE PATHWAYS.  

SciTech Connect (OSTI)

Radiation can damage cellular components, including DNA. Organisms have developed a panoply of means of dealing with DNA damage. Some repair paths have rather narrow substrate specificity (e.g. photolyases), which act on specific pyrimidine photoproducts in a specific type (e.g., DNA) and conformation (double-stranded B conformation) of nucleic acid. Others, for example, nucleotide excision repair, deal with larger classes of damages, in this case bulky adducts in DNA. A detailed discussion of DNA repair mechanisms is beyond the scope of this article, but one can be found in the excellent book of Friedberg et al. [1] for further detail. However, some DNA damages and paths for repair of those damages important for photobiology will be outlined below as a basis for the specific examples of genetic and molecular analysis that will be presented below.

SUTHERLAND, B.M.

2001-07-26T23:59:59.000Z

68

DNA heats up : Energetics of genome ejection from phage revealed by isothermal titration calorimetry  

E-Print Network [OSTI]

Most bacteriophages are known to inject their double-stranded DNA into bacteria upon receptor binding in an essentially spontaneous way. This downhill thermodynamic process from the intact virion toward the empty viral capsid plus released DNA is made possible by the energy stored during active packaging of the genome into the capsid. Only indirect measurements of this energy have been available until now using either single-molecule or osmotic suppression techniques. In this paper, we describe for the first time the use of isothermal titration calorimetry to directly measure the heat released (or equivalently the enthalpy) during DNA ejection from phage lambda, triggered in solution by a solubilized receptor. Quantitative analyses of the results lead to the identification of thermodynamic determinants associated with DNA ejection. The values obtained were found to be consistent with those previously predicted by analytical models and numerical simulations. Moreover, the results confirm the role of DNA hydration in the energetics of genome confinement in viral capsids.

Meerim Jeembaeva; B. Jönsson; Martin Castelnovo; Alex Evilevitch

2010-01-06T23:59:59.000Z

69

Mutation Research 684 (2010) 8189 Contents lists available at ScienceDirect  

E-Print Network [OSTI]

irradiation DNA double strand breaks Nitric oxide Cell proliferation a b s t r a c t Low-dose -particle irradiated by low-dose -particles (1­10 cGy) in a mixed system. Further studies indicated that nitric oxide exposures comprise 55% of the environmental dose to the human population and have been shown to induce

Yu, K.N.

70

Binary electrokinetic separation of target DNA from background DNA primers.  

SciTech Connect (OSTI)

This report contains the summary of LDRD project 91312, titled ''Binary Electrokinetic Separation of Target DNA from Background DNA Primers''. This work is the first product of a collaboration with Columbia University and the Northeast BioDefense Center of Excellence. In conjunction with Ian Lipkin's lab, we are developing a technique to reduce false positive events, due to the detection of unhybridized reporter molecules, in a sensitive and multiplexed detection scheme for nucleic acids developed by the Lipkin lab. This is the most significant problem in the operation of their capability. As they are developing the tools for rapidly detecting the entire panel of hemorrhagic fevers this technology will immediately serve an important national need. The goal of this work was to attempt to separate nucleic acid from a preprocessed sample. We demonstrated the preconcentration of kilobase-pair length double-stranded DNA targets, and observed little preconcentration of 60 base-pair length single-stranded DNA probes. These objectives were accomplished in microdevice formats that are compatible with larger detection systems for sample pre-processing. Combined with Columbia's expertise, this technology would enable a unique, fast, and potentially compact method for detecting/identifying genetically-modified organisms and multiplexed rapid nucleic acid identification. Another competing approach is the DARPA funded IRIS Pharmaceutical TIGER platform which requires many hours for operation, and an 800k$ piece of equipment that fills a room. The Columbia/SNL system could provide a result in 30 minutes, at the cost of a few thousand dollars for the platform, and would be the size of a shoebox or smaller.

James, Conrad D.; Derzon, Mark Steven

2005-10-01T23:59:59.000Z

71

Recognition of Double-Stranded RNA by Proteins  

E-Print Network [OSTI]

. Stephens Peter A. Beal University of Utah, Department of Chemistry, 315 South 1400 East, Room 2020, Salt level. In contrast, Correspondence to: Peter A. Beal; email: beal@chemistry. utah.edu). Contract grant

Beal, Peter A.

72

Dietary fish oil and butyrate increase apoptosis and decrease aberrant crypt foci in colon cancer by enhancing histone acetylation and p21waf1/cip1 expression  

E-Print Network [OSTI]

shrinkage, mitochondrial membrane blebbing, nuclear degradation, and DNA fragmentation (Shanmugathasan and Jothy, 2000). As the DNA fragments, the remaining double-strand breaks can be identified by gel electrophoresis, producing a specific pattern..., the nuclear chromatin condenses and DNA is degraded. Cellular remnants are packaged into apoptotic bodies, which are then phagocytized or sloughed into the passing lumenal contents (Vaux and Strasser, 1996). p21Waf1/Cip1 One of the earliest events...

Covert, Kristy Lynn

2006-08-16T23:59:59.000Z

73

The effect of magnesium ions on dielectric relaxation in semidilute DNA aqueous solutions  

E-Print Network [OSTI]

The effect of magnesium ion Mg2+ on the dielectric relaxation of semidilute DNA aqueous solutions has been studied by means of dielectric spectroscopy. Two dielectric relaxations in the 100 Hz - 100 MHz frequency range, originating in the motion of DNA counterions, were probed as a function of DNA and Mg2+ ion concentration in added MgCl2 salt. The high-frequency mode in the MHz range, stemming from the structural organization of the DNA network, reveals de Gennes-Pfeuty-Dobrynin correlation length as the pertinent fundamental length scale for sufficiently low concentration of added salt. No relaxation fingerprint of DNA denaturation bubbles, leading to exposed hydrophobic core scaling, was detected at low DNA concentrations, thus indicating an increased stability of the double-stranded conformation as compared to the case of DNA solutions with univalent counterions. The presence of Mg2+ does not change qualitatively the low frequency mode in the kHz range correlated with single DNA conformational properties....

Grgi?in, Danijel; Ivek, Tomislav; Tomi?, Silvia; Podgornik, Rudi

2013-01-01T23:59:59.000Z

74

Crystal Structure of the Human NKX2.5 Homeodomain in Complex with DNA Target  

SciTech Connect (OSTI)

NKX2.5 is a homeodomain containing transcription factor regulating cardiac formation and function, and its mutations are linked to congenital heart disease. Here we provide the first report of the crystal structure of the NKX2.5 homeodomain in complex with double-stranded DNA of its endogenous target, locating within the proximal promoter -242 site of the atrial natriuretic factor gene. The crystal structure, determined at 1.8 {angstrom} resolution, demonstrates that NKX2.5 homeodomains occupy both DNA binding sites separated by five nucleotides without physical interaction between themselves. The two homeodomains show identical conformation despite the differences in the DNA sequences they bind, and no significant bending of the DNA was observed. Tyr54, absolutely conserved in NK2 family proteins, mediates sequence-specific interaction with the TAAG motif. This high resolution crystal structure of NKX2.5 protein provides a detailed picture of protein and DNA interactions, which allows us to predict DNA binding of mutants identified in human patients.

Pradhan, Lagnajeet; Genis, Caroli; Scone, Peyton; Weinberg, Ellen O.; Kasahara, Hideko; Nam, Hyun-Joo (BU-M); (Florida); (Texas)

2012-10-16T23:59:59.000Z

75

Breaking Earth Poems  

E-Print Network [OSTI]

CALIFORNIA RIVERSIDE Breaking Earth Poems A Thesis submittedFestival……………………………………………………………………..…..14 Earth Against Mylittle else in their hands. Earth Against My Back I lay in

Hernandez, Scott Mcnaul

2012-01-01T23:59:59.000Z

76

Free energy landscape and characteristic forces for the initiation of DNA unzipping  

E-Print Network [OSTI]

DNA unzipping, the separation of its double helix into single strands, is crucial in modulating a host of genetic processes. Although the large-scale separation of double-stranded DNA has been studied with a variety of theoretical and experimental techniques, the minute details of the very first steps of unzipping are still unclear. Here, we use atomistic molecular dynamics (MD) simulations, coarse-grained simulations and a statistical-mechanical model to study the initiation of DNA unzipping by an external force. The calculation of the potential of mean force profiles for the initial separation of the first few terminal base pairs in a DNA oligomer reveal that forces ranging between 130 and 230 pN are needed to disrupt the first base pair, values of an order of magnitude larger than those needed to disrupt base pairs in partially unzipped DNA. The force peak has an "echo," of approximately 50 pN, at the distance that unzips the second base pair. We show that the high peak needed to initiate unzipping derives...

Mentes, Ahmet; Brunk, Elizabeth; Wereszczynski, Jeff; Joyeux, Marc; Andricioaei, Ioan

2015-01-01T23:59:59.000Z

77

Free energy landscape and characteristic forces for the initiation of DNA unzipping  

E-Print Network [OSTI]

DNA unzipping, the separation of its double helix into single strands, is crucial in modulating a host of genetic processes. Although the large-scale separation of double-stranded DNA has been studied with a variety of theoretical and experimental techniques, the minute details of the very first steps of unzipping are still unclear. Here, we use atomistic molecular dynamics (MD) simulations, coarse-grained simulations and a statistical-mechanical model to study the initiation of DNA unzipping by an external force. The calculation of the potential of mean force profiles for the initial separation of the first few terminal base pairs in a DNA oligomer reveal that forces ranging between 130 and 230 pN are needed to disrupt the first base pair, values of an order of magnitude larger than those needed to disrupt base pairs in partially unzipped DNA. The force peak has an "echo," of approximately 50 pN, at the distance that unzips the second base pair. We show that the high peak needed to initiate unzipping derives from a free energy basin that is distinct from the basins of subsequent base pairs because of entropic contributions and we highlight the microscopic origin of the peak. Our results suggest a new window of exploration for single molecule experiments.

Ahmet Mentes; Ana Maria Florescu; Elizabeth Brunk; Jeff Wereszczynski; Marc Joyeux; Ioan Andricioaei

2015-01-27T23:59:59.000Z

78

Line narrowing spectroscopic studies of DNA-carcinogen adducts and DNA-dye complexes  

SciTech Connect (OSTI)

Laser-induced fluorescence line narrowing and non-line narrowing spectroscopic methods were applied to conformational studies of stable DNA adducts of the 7{beta}, 8{alpha}-dihydoxy-9{alpha}, l0{alpha}-epoxy-7,8,9, 10-tetrahydrobenzo[{alpha}]pyrene (anti-BPDE). Stereochemically distinct (+)-trans-, ({minus})-trans-, (+)-cis- and ({minus})-cis adducts of anti-BPDE bound to exocyclic amino group of the central guanine in an 11-mer oligonucleotide, exist in a mixture of conformations in frozen aqueous buffer matrices. The (+)-trans adduct adopts primarily an external conformation with a smaller fraction ( {approximately} 25 %) exists in a partially base-stacked conformation. Both cis adducts were found to be intercalated with significant {pi}-{pi} stacking interactions between the pyrenyl residues and the bases. Conformations of the trans-adduct of (+)-anti -BPDE in 11-mer oligonucleotides were studied as a function of flanking bases. In single stranded form the adduct at G{sub 2} or G{sub 3} (5 ft-flanking, base guanine) adopts a conformation with strong, interaction with the bases. In contrast, the adduct with a 5ft-flanking, thymine exists in a primarily helixexternal conformation. Similar differences were observed in the double stranded oligonucleotides. The nature of the 3ft-flanking base has little influence on the conformational equilibrium of the (+)-trans-anti BPDE-dG adduct. The formation and repair of BPDE-N{sup 2}-dG in DNA isolated from the skin of mice treated topically with benzo[{alpha}]pyrene (BP) was studied. Low-temperature fluorescence spectroscopy of the intact DNA identified the major adduct as (+)-trans-anti-BPDE-N-dG, and the minor adduct fraction consisted mainly of (+)-cis-anti-BPDE-N{sup 2}-dG.

Suh, Myungkoo

1995-12-06T23:59:59.000Z

79

Crystal structures of [lamda] exonuclease in complex with DNA suggest an electrostatic ratchet mechanism for processivity  

SciTech Connect (OSTI)

The {lambda} exonuclease is an ATP-independent enzyme that binds to dsDNA ends and processively digests the 5'-ended strand to form 5' mononucleotides and a long 3' overhang. The crystal structure of {lambda} exonuclease revealed a toroidal homotrimer with a central funnel-shaped channel for tracking along the DNA, and a mechanism for processivity based on topological linkage of the trimer to the DNA was proposed. Here, we have determined the crystal structure of {lambda} exonuclease in complex with DNA at 1.88-{angstrom} resolution. The structure reveals that the enzyme unwinds the DNA prior to cleavage, such that two nucleotides of the 5'-ended strand insert into the active site of one subunit of the trimer, while the 3'-ended strand passes through the central channel to emerge out the back of the trimer. Unwinding of the DNA is facilitated by several apolar residues, including Leu78, that wedge into the base pairs at the single/double-strand junction to form favorable hydrophobic interactions. The terminal 5' phosphate of the DNA binds to a positively charged pocket buried at the end of the active site, while the scissile phosphate bridges two active site Mg{sup 2+} ions. Our data suggest a mechanism for processivity in which wedging of Leu78 and other apolar residues into the base pairs of the DNA restricts backward movement, whereas attraction of the 5' phosphate to the positively charged pocket drives forward movement of the enzyme along the DNA at each cycle of the reaction. Thus, processivity of {lambda} exonuclease operates not only at the level of the trimer, but also at the level of the monomer.

Zhang, Jinjin; McCabe, Kimberly A.; Bell, Charles E. (OSU)

2011-08-10T23:59:59.000Z

80

Design of a combinatorial dna microarray for protein-dnainteraction studies  

SciTech Connect (OSTI)

Background: Discovery of precise specificity oftranscription factors is an important step on the way to understandingthe complex mechanisms of gene regulation in eukaryotes. Recently,doublestranded protein-binding microarrays were developed as apotentially scalable approach to tackle transcription factor binding siteidentification. Results: Here we present an algorithmic approach toexperimental design of a microarray that allows for testing fullspecificity of a transcription factor binding to all possible DNA bindingsites of a given length, with optimally efficient use of the array. Thisdesign is universal, works for any factor that binds a sequence motif andis not species-specific. Furthermore, simulation results show that dataproduced with the designed arrays is easier to analyze and would resultin more precise identification of binding sites. Conclusion: In thisstudy, we present a design of a double stranded DNA microarray forprotein-DNA interaction studies and show that our algorithm allowsoptimally efficient use of the arrays for this purpose. We believe such adesign will prove useful for transcription factor binding siteidentification and other biological problems.

Mintseris, Julian; Eisen, Michael B.

2006-07-07T23:59:59.000Z

Note: This page contains sample records for the topic "dna double-strand breaks" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


81

Finite group symmetry breaking  

E-Print Network [OSTI]

Finite group symmetry is commonplace in Physics, in particular through crystallographic groups occurring in condensed matter physics -- but also through the inversions (C,P,T and their combinations) occurring in high energy physics and field theory. The breaking of finite groups symmetry has thus been thoroughly studied, and general approaches exist to investigate it. In Landau theory, the state of a system is described by a finite dimensional variable (the {\\it order parameter}), and physical states correspond to minima of a potential, invariant under a group. In this article we describe the basics of symmetry breaking analysis for systems described by a symmetric polynomial; in particular we discuss generic symmetry breakings, i.e. those determined by the symmetry properties themselves and independent on the details of the polynomial describing a concrete system. We also discuss how the plethora of invariant polynomials can be to some extent reduced by means of changes of coordinates, i.e. how one can reduce to consider certain types of polynomials with no loss of generality. Finally, we will give some indications on extension of this theory, i.e. on how one deals with symmetry breakings for more general groups and/or more general physical systems.

G. Gaeta

2005-10-02T23:59:59.000Z

82

SuperBreak: Using Interactivity to Enhance Ergonomic Typing Breaks  

E-Print Network [OSTI]

SuperBreak: Using Interactivity to Enhance Ergonomic Typing Breaks Dan Morris, A.J. Bernheim Brush Repetitive strain injuries and ergonomics concerns have become increasingly significant health issues software mechanisms exist for managing ergonomics; the most well-known are "break-reminder" packages

Rajamani, Sriram K.

83

$R$-Parity Breaking Phenomenology  

E-Print Network [OSTI]

We review various features of the $R$-parity breaking phenomenology, with particular attention to the low energy observables, and to the patterns of the $R$-parity breaking interactions that arise in Grand Unified models.

Francesco Vissani

1996-03-11T23:59:59.000Z

84

Models of Dynamical Supersymmetry Breaking  

E-Print Network [OSTI]

We review a class of models of dynamical supersymmetry breaking, and give a unified description of these models.

Lisa Randall

1997-06-23T23:59:59.000Z

85

Self-avoiding worm-like chain model for dsDNA loop formation  

E-Print Network [OSTI]

We compute for the first time the effects of excluded volume on the probability for double-stranded DNA to form a loop. We utilize a Monte-Carlo algorithm for generation of large ensembles of self- avoiding worm-like chains, which are used to compute the J-factor for varying lengthscales. In the entropic regime, we confirm the scaling-theory prediction of a power-law drop off of -1.92, which is significantly stronger than the -1.5 power-law predicted by the non-self-avoiding worm-like chain model. In the elastic regime, we find that the angle-independent end-to-end chain distribution is highly anisotropic. This anisotropy, combined with the excluded volume constraints, lead to an increase in the J-factor of the self-avoiding worm-like chain by about half an order of magnitude relative to its non-self-avoiding counterpart. This increase could partially explain the anomalous results of recent cyclization experiments, in which short dsDNA molecules were found to have an increased propensity to form a loop.

Yaroslav Pollak; Sarah Goldberg; Roee Amit

2014-11-06T23:59:59.000Z

86

Nanotechnology with DNA DNA Nanodevices  

E-Print Network [OSTI]

Nanotechnology with DNA DNA Nanodevices Friedrich C. Simmel* and Wendy U. Dittmer A DNA actuator. Introduction.............285 2. Overview: DNA Nanotechnology.......285 3. Prototypes of Nanomechanical DNA overview of DNA nanotechnology as a whole is given. The most important properties of DNA molecules

Ludwig-Maximilians-Universität, München

87

APPROVED Tie-Breaking Procedures  

E-Print Network [OSTI]

a public school applicant attends a low API school (1-4), or if a private school to the rest of the tie-break pool. Spring Admission Applicants recommended, relative to the rest of the tie-break pool. #12;APPROVED Denied Admission

Iglesia, Enrique

88

The structural basis for partitioning of the XRCC1/DNA ligase III-[alpha] BRCT-mediated dimer complexes  

SciTech Connect (OSTI)

The ultimate step common to almost all DNA repair pathways is the ligation of the nicked intermediate to form contiguous double-stranded DNA. In the mammalian nucleotide and base excision repair pathways, the ligation step is carried out by ligase III-{alpha}. For efficient ligation, ligase III-{alpha} is constitutively bound to the scaffolding protein XRCC1 through interactions between the C-terminal BRCT domains of each protein. Although structural data for the individual domains has been available, no structure of the complex has been determined and several alternative proposals for this interaction have been advanced. Interpretation of the models is complicated by the formation of homodimers that, depending on the model, may either contribute to, or compete with heterodimer formation. We report here the structures of both homodimer complexes as well as the heterodimer complex. Structural characterization of the heterodimer formed from a longer XRCC1 BRCT domain construct, including residues comprising the interdomain linker region, revealed an expanded heterodimer interface with the ligase III-{alpha} BRCT domain. This enhanced linker-mediated binding interface plays a significant role in the determination of heterodimer/homodimer selectivity. These data provide fundamental insights into the structural basis of BRCT-mediated dimerization, and resolve questions related to the organization of this important repair complex.

Cuneo, Matthew J.; Gabel, Scott A.; Krahn, Joseph M.; Ricker, Melissa A.; London, Robert E. (NIH)

2011-11-17T23:59:59.000Z

89

The Structural Basis for Partitioning of the XRCC1/DNA Ligase III-alpha BRCT-mediated Dimer Complexes  

SciTech Connect (OSTI)

The ultimate step common to almost all DNA repair pathways is the ligation of the nicked intermediate to form contiguous double-stranded DNA. In the mammalian nucleotide and base excision repair pathways, the ligation step is carried out by ligase III-{alpha}. For efficient ligation, ligase III-{alpha} is constitutively bound to the scaffolding protein XRCC1 through interactions between the C-terminal BRCT domains of each protein. Although structural data for the individual domains has been available, no structure of the complex has been determined and several alternative proposals for this interaction have been advanced. Interpretation of the models is complicated by the formation of homodimers that, depending on the model, may either contribute to, or compete with heterodimer formation. We report here the structures of both homodimer complexes as well as the heterodimer complex. Structural characterization of the heterodimer formed from a longer XRCC1 BRCT domain construct, including residues comprising the interdomain linker region, revealed an expanded heterodimer interface with the ligase III-{alpha} BRCT domain. This enhanced linker-mediated binding interface plays a significant role in the determination of heterodimer/homodimer selectivity. These data provide fundamental insights into the structural basis of BRCT-mediated dimerization, and resolve questions related to the organization of this important repair complex.

M Cuneo; S Gabel; J Krahn; M Ricker; R London

2011-12-31T23:59:59.000Z

90

Solvatochromism and time-resolved fluorescence of the antitumor agent mitoxantrone and its analogues in solution and in DNA  

SciTech Connect (OSTI)

The electronic spectroscopy and fluorescence kinetics of 1,4-dihydroxy-5,8-(2-(2-((2-hydroxyethyl)amino)ethyl)amino)-9,10-anthracenedione (mitoxantrone) and three closely related analogues have been studied in several solvents. The small solvatochromic blue shifts of their visible charge-transfer absorption bands in protic solvents are dominated by interactions with a solvent H-bonding donor, rather than by dipole-dielectric solute-solvent electrostatics. These interactions are unrelated to the phenolic hydroxy groups or the distal N atoms on the side chains but must be localized to the carbonyl groups. The fluorescence decays of all four anthraquinones are controlled by subnanosecond nonradiative relaxation in all solvents studied. At least two decay mechanisms contribute to the observed fluorescence kinetics in solution: (a) subnanosecond internal conversion that is accelerated relative to that in 1,4-diaminoanthraquinone by the presence of the flexible 1,4-side chains in mitoxantrone and its analogues; (b) an additional decay mode that is accentuated in H-bonding solvents. A substantial normal isotope effect occurs in the fluorescence lifetimes of mitoxantrone in perdeuterated water and methanol but not in aprotic solvents. When bound to double-stranded calf thymus DNA, mitoxantrone displays a fluorescence lifetime similar to that in aprotic solvents, suggesting that H-bonding interactions with water are precluded by chromophore intercalation. DNA-bound ametantrone exhibits a lifetime longer than that in either H-bonding or aprotic solvents, indicating that immobilization of the side chains through binding of the distal N atoms to the DNA backbone may influence the decay kinetics. This technique therefore shows potential for elucidating the DNA binding modes for a large class of intercalative drugs.

Su Lin; Struve, W.S. (Ames Lab., IA (United States))

1991-03-21T23:59:59.000Z

91

Chiral Symmetry Breaking in Graphene  

E-Print Network [OSTI]

The question of whether the Coulomb interaction is strong enough to break the sublattice symmetry of un-doped graphene is discussed. We formulate a strong coupling expansion where the ground state of the Coulomb Hamiltonian is found exactly and the kinetic hopping Hamiltonian is treated as a perturbation. We argue that many of the properties of the resulting system would be shared by graphene with a Hubbard model interaction. In particular, the best candidate sublattice symmetry breaking ground state is an antiferromagnetic Mott insulator. We discuss the results of some numerical simulations which indicate that the Coulomb interaction is indeed subcritical. We also point out the curious fact that, if the electron did not have spin degeneracy, the tendency to break chiral symmetry would be much greater and even relatively weak Coulomb interactions would likely gap the spectrum.

Gordon W. Semenoff

2011-08-19T23:59:59.000Z

92

Small Break Air Ingress Experiment  

SciTech Connect (OSTI)

The small break air-ingress experiment, described in this report, is designed to investigate air-ingress phenomena postulated to occur in pipes in a very high temperature gas-cooled reactor (VHTRs). During this experiment, air-ingress rates were measured for various flow and break conditions through small holes drilled into a pipe of the experimental apparatus. The holes were drilled at right angles to the pipe wall such that a direction vector drawn from the pipe centerline to the center of each hole was at right angles with respect to the pipe centerline. Thus the orientation of each hole was obtained by measuring the included angle between the direction vector of each hole with respect to a reference line anchored on the pipe centerline and pointing in the direction of the gravitational force. Using this reference system, the influence of several important parameters on the air ingress flow rate were measured including break orientation, break size, and flow velocity . The approach used to study the influence of these parameters on air ingress is based on measuring the changes in oxygen concentrations at various locations in the helium flow circulation system as a function of time using oxygen sensors (or detectors) to estimate the air-ingress rates through the holes. The test-section is constructed of a stainless steel pipe which had small holes drilled at the desired locations.

Chang Oh; Eung Soo Kim

2011-09-01T23:59:59.000Z

93

CTRLWORK AND MACBREAKZ ERGONOMIC BREAK REMINDER SOFTWARE  

E-Print Network [OSTI]

CTRLWORK AND MACBREAKZ ERGONOMIC BREAK REMINDER SOFTWARE SOFTWARE FEATURES: Analyzes your for installation assistance. 1. Please uninstall the former ergonomic break software, RSIGuard, if currently instructions on the screen. For more information, contact the UCSB Ergonomics Program at Ergonomics

California at Santa Barbara, University of

94

DNA demethylation by DNA repair  

E-Print Network [OSTI]

Active DNA demethylation underlies key facets of reproduction in flowering plants and mammals and serves a general genome housekeeping function in plants. A family of 5-methylcytosine DNA glycosylases catalyzes plant ...

Gehring, Mary

95

Function of the ATR N-terminal domain revealed by an ATM/ATR chimera  

SciTech Connect (OSTI)

The ATM and ATR kinases function at the apex of checkpoint signaling pathways. These kinases share significant sequence similarity, phosphorylate many of the same substrates, and have overlapping roles in initiating cell cycle checkpoints. However, they sense DNA damage through distinct mechanisms. ATR primarily senses single stranded DNA (ssDNA) through its interaction with ATRIP, and ATM senses double strand breaks through its interaction with Nbs1. We determined that the N-terminus of ATR contains a domain that binds ATRIP. Attaching this domain to ATM allowed the fusion protein (ATM*) to bind ATRIP and associate with RPA-coated ssDNA. ATM* also gained the ability to localize efficiently to stalled replication forks as well as double strand breaks. Despite having normal kinase activity when tested in vitro and being phosphorylated on S1981 in vivo, ATM* is defective in checkpoint signaling and does not complement cellular deficiencies in either ATM or ATR. These data indicate that the N-terminus of ATR is sufficient to bind ATRIP and to promote localization to sites of replication stress.

Chen Xinping [Department of Biochemistry, Vanderbilt University, 613 Light Hall, 23rd, Pierce Avenue, Nashville, TN 37232 (United States); Zhao Runxiang [Department of Biochemistry, Vanderbilt University, 613 Light Hall, 23rd, Pierce Avenue, Nashville, TN 37232 (United States); Glick, Gloria G. [Department of Biochemistry, Vanderbilt University, 613 Light Hall, 23rd, Pierce Avenue, Nashville, TN 37232 (United States); Cortez, David [Department of Biochemistry, Vanderbilt University, 613 Light Hall, 23rd, Pierce Avenue, Nashville, TN 37232 (United States)]. E-mail: david.cortez@vanderbilt.edu

2007-05-01T23:59:59.000Z

96

Breaking down the bubbly | EMSL  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr May JunDatastreamsmmcrcalgovInstrumentsruc DocumentationP-Series to someone6 M.ExtracellularBradbury Science MuseumBrainBreaking a

97

Issues in standard model symmetry breaking  

SciTech Connect (OSTI)

This work discusses the symmetry breaking sector of the SU(2) x U(1) electroweak model. The first two chapters discuss Higgs masses in two simple Higgs models. The author proves low-enery theorems for the symmetry breaking sector: The threshold behavior of gauge-boson scattering is completely determined, whenever the symmetry breaking sector meets certain simple conditions. The author uses these theorems to derive event rates for the superconducting super collider (SSC). The author shows that the SSC may be able to determine whether the interactions of the symmetry breaking sector are strong or weak. 54 refs.

Golden, M.

1988-04-01T23:59:59.000Z

98

Electroweak Symmetry Breaking via QCD  

E-Print Network [OSTI]

We propose a new mechanism to generate the electroweak scale within the framework of QCD, which is extended to include conformally invariant scalar degrees of freedom belonging to a larger irreducible representation of $SU(3)_c$. The electroweak symmetry breaking is triggered dynamically via the Higgs portal by the condensation of the colored scalar field around 1 TeV. The mass of the colored boson is restricted to be 350 GeV $\\lesssim m_S\\lesssim $ 3 TeV, with the upper bound obtained from perturbative renormalization group evolution. This implies that the colored boson can be produced at LHC. If the colored boson is electrically charged, the branching fraction of the Higgs decaying into two photons can slightly increase, and moreover, it can be produced at future linear colliders. Our idea of non-perturbative EW scale generation can serve as a new starting point for more realistic model building in solving the hierarchy problem.

Jisuke Kubo; Kher Sham Lim; Manfred Lindner

2014-09-01T23:59:59.000Z

99

Degeneracy Breaking of Hydrogen Atom  

E-Print Network [OSTI]

The three dimensional rotation group, SO(3), is a symmetry group of the normal hydrogen atom. Each reducible representation of this group can be associated with a degenerate energy level. If this atom is placed in an external magnetic field, the interaction between the orbital magnetic moment with this field will lead to a symmetry breaking where the symmetry group of the atom is a new group distinct from the SO(3) group. This phenomenon describes the normal Zeeman effect, where a degenerate energy level splits into several new energy levels. It is explicitly shown that each of the new energy levels can be associated with an irreducible representation of the new symmetry group.

Agung Trisetyarso; Pantur Silaban

2014-11-21T23:59:59.000Z

100

Alan Turing as a Statistician: Breaking the Code by Breaking the Bayesian Egg  

E-Print Network [OSTI]

Alan Turing as a Statistician: Breaking the Code by Breaking the Bayesian Egg Joseph K. Blitzstein, Harvard University Alan Turing is widely considered the father of computer science. Yet even

Wolfe, Patrick J.

Note: This page contains sample records for the topic "dna double-strand breaks" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


101

Vanadium catalysts break down biomass for fuels  

E-Print Network [OSTI]

- 1 - Vanadium catalysts break down biomass for fuels March 26, 2012 Vanadium catalysts break down biomass into useful components Due to diminishing petroleum reserves, non-food biomass (lignocellulose biomass into high-value commodity chemicals. The journal Angewandte Chemie International Edition published

102

Gauge symmetry breaking in orbifold model building  

E-Print Network [OSTI]

We review the gauge symmetry breaking mechanism due to orbifold projections in orbifold model building. We explicitly show the existence of a scale of breaking if such a symmetry breaking is due to freely-acting orbifold operators only, i.e. in case the breaking is realized non-locally in the internal space. We show that such a scale is related to the compactification moduli only, and that there are no extra continuous parameters, at least in semirealistic models with N=1 SUSY in four dimensions. In this sense, the mechanism is peculiarly different from the standard Higgs (or Hosotani) symmetry breaking mechanism. We show that the mechanism also differs from that present in standard orbifold models where, even in presence of discrete Wilson lines, a scale of breaking is generically missing, since the breaking is localized in specific points in the internal space. We review a set of background geometries where the described non-local breaking is realized, both in the case of two and six extra dimensions. In the latter case, relevant in string model building, we consider both heterotic and open string compactifications.

Michele Trapletti

2006-11-02T23:59:59.000Z

103

Explicit versus Spontaneous Diffeomorphism Breaking in Gravity  

E-Print Network [OSTI]

Gravitational theories with fixed background fields break local Lorentz and diffeomorphism invariance either explicitly or spontaneously. In the case of explicit breaking it is known that conflicts can arise between the dynamics and geometrical constraints, while spontaneous breaking evades this problem. It is for this reason that in the gravity sector of the Standard-Model Extension (SME) it is assumed that the background fields (SME coefficients) originate from spontaneous symmetry breaking. However, in other examples, such as Chern-Simons gravity and massive gravity, diffeomorphism invariance is explicitly broken by the background fields, and the potential conflicts between the dynamics and geometry can be avoided in most cases. An analysis of how this occurs is given, and the conditions that are placed on the metric tensor and gravitational structure as a result of the presence of an explicit-breaking background are described. The gravity sector of the SME is then considered for the case of explicit breaking. However, it is found that a useful post-Newtonian limit is only obtained when the symmetry breaking is spontaneous.

Robert Bluhm

2015-04-02T23:59:59.000Z

104

Airborne observations of the kinematics and statistics of breaking waves  

E-Print Network [OSTI]

v 3 Observations of wave breaking kinematics in fetch-crest length . . . . . C.6 Wave elevation . . . . . . . .breaking waves in the images . . . . . . . . . . . 3.3.3

Kleiss, Jessica M.

2009-01-01T23:59:59.000Z

105

Break-out Discussion i: Modeling Consumer Behavior Residential...  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

Break-out Discussion i: Modeling Consumer Behavior Residential Scale Break-out Discussion i: Modeling Consumer Behavior Residential Scale This presentaion summarizes the...

106

Breaking Parity Symmetry Using Extra Dimensions  

E-Print Network [OSTI]

We present a new way to break parity symmetry in left-right symmetric models using boundary conditions on the fields residing in the fifth dimension. We also discuss the connection between the limits on the size of extra dimensions and the scale of right handed symmetry breaking obtained from the analysis of neutrinoless double beta decay in the case where the righthanded gauge symmetry is in the bulk.

R. N. Mohapatra; A. Pérez-Lorenzana

1999-11-17T23:59:59.000Z

107

Genes and structural proteins of the phage SYN5 of the marine cyanobacteria, Synechococcus  

E-Print Network [OSTI]

Bacteriophage have been proposed to be the most abundant organisms on the planet, at an estimated 10³¹ particles globally (Hendrix et al., 1999). The majority of bacteriophage isolates (96%) are double-stranded DNA tailed ...

Pope, Welkin Hazel

2005-01-01T23:59:59.000Z

108

Laboratory Directed Research and Development Program. Annual report  

SciTech Connect (OSTI)

This report briefly discusses the following research: Advances in Geoexploration; Transvenous Coronary Angiography with Synchrotron X-Rays; Borehole Measurements of Global Warming; Molecular Ecology: Development of Field Methods for Microbial Growth Rate and Activity Measurements; A New Malaria Enzyme - A Potential Source for a New Diagnostic Test for Malaria and a Target for a New Antimalarial Drug; Basic Studies on Thoron and Thoron Precursors; Cloning of the cDNA for a Human Serine/Threonine Protein Kinase that is Activated Specifically by Double-Stranded DNA; Development of an Ultra-Fast Laser System for Accelerator Applications; Cluster Impact Fusion; Effect of a Bacterial Spore Protein on Mutagenesis; Structure and Function of Adenovirus Penton Base Protein; High Resolution Fast X-Ray Detector; Coherent Synchrotron Radiation Longitudinal Bunch Shape Monitor; High Grain Harmonic Generation Experiment; BNL Maglev Studies; Structural Investigations of Pt-Based Catalysts; Studies on the Cellular Toxicity of Cocaine and Cocaethylene; Human Melanocyte Transformation; Exploratory Applications of X-Ray Microscopy; Determination of the Higher Ordered Structure of Eukaryotic Chromosomes; Uranium Neutron Capture Therapy; Tunneling Microscopy Studies of Nanoscale Structures; Nuclear Techiques for Study of Biological Channels; RF Sources for Accelerator Physics; Induction and Repair of Double-Strand Breaks in the DNA of Human Lymphocytes; and An EBIS Source of High Charge State Ions up to Uranium.

Ogeka, G.J.; Romano, A.J.

1992-12-01T23:59:59.000Z

109

Laboratory Directed Research and Development Program  

SciTech Connect (OSTI)

This report briefly discusses the following research: Advances in Geoexploration; Transvenous Coronary Angiography with Synchrotron X-Rays; Borehole Measurements of Global Warming; Molecular Ecology: Development of Field Methods for Microbial Growth Rate and Activity Measurements; A New Malaria Enzyme - A Potential Source for a New Diagnostic Test for Malaria and a Target for a New Antimalarial Drug; Basic Studies on Thoron and Thoron Precursors; Cloning of the cDNA for a Human Serine/Threonine Protein Kinase that is Activated Specifically by Double-Stranded DNA; Development of an Ultra-Fast Laser System for Accelerator Applications; Cluster Impact Fusion; Effect of a Bacterial Spore Protein on Mutagenesis; Structure and Function of Adenovirus Penton Base Protein; High Resolution Fast X-Ray Detector; Coherent Synchrotron Radiation Longitudinal Bunch Shape Monitor; High Grain Harmonic Generation Experiment; BNL Maglev Studies; Structural Investigations of Pt-Based Catalysts; Studies on the Cellular Toxicity of Cocaine and Cocaethylene; Human Melanocyte Transformation; Exploratory Applications of X-Ray Microscopy; Determination of the Higher Ordered Structure of Eukaryotic Chromosomes; Uranium Neutron Capture Therapy; Tunneling Microscopy Studies of Nanoscale Structures; Nuclear Techiques for Study of Biological Channels; RF Sources for Accelerator Physics; Induction and Repair of Double-Strand Breaks in the DNA of Human Lymphocytes; and An EBIS Source of High Charge State Ions up to Uranium.

Ogeka, G.J.; Romano, A.J.

1992-12-01T23:59:59.000Z

110

New Mechanisms of Gauge-Mediated Supersymmetry Breaking  

E-Print Network [OSTI]

We review the motivation for Gauge-Mediated Supersymmetry Breaking and discuss some recent advances.

Lisa Randall

1997-06-23T23:59:59.000Z

111

Chimera Death: Symmetry Breaking in Dynamical Networks  

E-Print Network [OSTI]

For a network of generic oscillators with nonlocal topology and symmetry-breaking coupling we establish novel partially coherent inhomogeneous spatial patterns, which combine the features of chimera states (coexisting incongruous coherent and incoherent domains) and oscillation death (oscillation suppression), which we call chimera death. We show that due to the interplay of nonlocality and breaking of rotational symmetry by the coupling two distinct scenarios from oscillatory behavior to a stationary state regime are possible: a transition from an amplitude chimera to chimera death via in-phase synchronized oscillations, and a direct abrupt transition for larger coupling strength.

Anna Zakharova; Marie Kapeller; Eckehard Schöll

2014-02-03T23:59:59.000Z

112

Symmetry-Breaking Constraints for Matrix Models  

E-Print Network [OSTI]

-breaking constraints. Experimental re- sults confirm their value. 1 Introduction Symmetry in a CSP model is an important issue as the exploration of symmet- ric but essentially equivalent branches in a search tree may techniques have been developed to address the issue of eliminating symmetry in CSP models. An important class

Flener, Pierre

113

REPLACE YOUR MERCURY THERMOMETERS BEFORE THEY BREAK!  

E-Print Network [OSTI]

REPLACE YOUR MERCURY THERMOMETERS BEFORE THEY BREAK! Did you know, mercury from broken thermometers to the local environment, if broken thermometers in sinks eventually end at the sanitary sewer plant. Broken mercury thermometers create hazardous waste that is costly to clean up and costly to dispose of. Other

114

Asiakastyytyväisyystutkimus - DNA Kauppa Lappeenranta.  

E-Print Network [OSTI]

??Opinnäytetyön aiheena oli DNA Kauppa Lappeenrannan asiakastyytyväisyyden tutkiminen. Tutkimuksen ensisijaisena tavoitteena oli selvittää, kuinka tyytyväisiä asiakkaat olivat olleet DNA Kauppa Lappeenrannan ja asiakkaan väliseen kontaktipintaan… (more)

Pitkänen, Jesse

2013-01-01T23:59:59.000Z

115

Sturdier DNA Nanotubes via Ligation Patrick O'Neill, Paul W. K. Rothemund, Ashish Kumar, and D. K. Fygenson*,,  

E-Print Network [OSTI]

Sturdier DNA Nanotubes via Ligation Patrick O'Neill, Paul W. K. Rothemund, Ashish Kumar, and D. K, California 93106 Received February 15, 2006; Revised Manuscript Received May 19, 2006 ABSTRACT DNA nanotubes nanotubes begin to melt at temperatures below 40 °C, break open when deposited on mica or scanned by AFM

Winfree, Erik

116

Winter Break 2011 (January) Knowledge is Power Program  

E-Print Network [OSTI]

, TX) · Kentucky Equine Humane Center (Nicholsville, Kentucky) · Grand Canyon: Kane Ranch (Marble participants) · Community Collaborations, Flood Relief (Atlanta, GA) (10 participants) Weekend Breaks Fall 2009, San Juan, TX (10 participants) Weekend Break Fall 2008 (October)-12 participants · Cedar Rapids

117

Reddit AMA: Marius Stan, energy researcher and Breaking Bad actor...  

Broader source: Energy.gov (indexed) [DOE]

Reddit AMA: Marius Stan, energy researcher and Breaking Bad actor Reddit AMA: Marius Stan, energy researcher and Breaking Bad actor February 14, 2013 - 12:36pm Addthis Marius Stan,...

118

Wind Power Reliability: Breaking Down a Barrier | Department...  

Broader source: Energy.gov (indexed) [DOE]

Wind Power Reliability: Breaking Down a Barrier Wind Power Reliability: Breaking Down a Barrier June 25, 2010 - 12:16pm Addthis EnerNex Corporation is developing documentation and...

119

Housing and Dining Services Spring Break Information March 2011  

E-Print Network [OSTI]

charge to stay over spring break. Kitchenettes will be open in all residence halls over the break period equipment · Refrigerators with food may be left on · Close and lock your windows and doors · Turn off your

120

Laboratory Studies of Nonlinear and Breaking Surface Waves  

E-Print Network [OSTI]

A. Breaking-wave generation . . . . . . . . . . . . . . . .of the wave tank. . . . . . . . . . . . . . . . . . . .On steep gravity waves meeting a vertical wall: a triple

Drazen, David

2006-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "dna double-strand breaks" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


121

Gravity dual of metastable dynamical supersymmetry breaking  

SciTech Connect (OSTI)

Metastable, supersymmetry-breaking configurations can be created in flux geometries by placing antibranes in warped throats. Via gauge/gravity duality, such configurations should have an interpretation as supersymmetry-breaking states in the dual field theory. In this paper, we perturbatively determine the asymptotic supergravity solutions corresponding to D3-brane probes placed at the tip of the cascading warped deformed conifold geometry, which is dual to an SU(N+M)xSU(N) gauge theory. The backreaction of the antibranes has the effect of introducing imaginary anti-self-dual flux, squashing the compact part of the space and forcing the dilaton to run. Using the generalization of holographic renormalization to cascading geometries, we determine the expectation values of operators in the dual field theory in terms of the asymptotic values of the supergravity fields.

DeWolfe, Oliver [Department of Physics, 390 UCB, University of Colorado, Boulder, Colorado 80309 (United States); Kachru, Shamit; Mulligan, Michael [Department of Physics and SLAC, Stanford University, Stanford, California 94305/94309 (United States)

2008-03-15T23:59:59.000Z

122

Textural break foundation wall construction modules  

DOE Patents [OSTI]

Below-grade, textural-break foundation wall structures are provided for inhibiting diffusion and advection of liquids and gases into and out from a surrounding hydrogeologic environment. The foundation wall structure includes a foundation wall having an interior and exterior surface and a porous medium disposed around a portion of the exterior surface. The structure further includes a modular barrier disposed around a portion of the porous medium. The modular barrier is substantially removable from the hydrogeologic environment.

Phillips, Steven J. (Kennewick, WA)

1990-01-01T23:59:59.000Z

123

Dec. 13-Jan. 3 Winter Break  

E-Print Network [OSTI]

Dec. 13-Jan. 3 Winter Break Students must be out of residence halls by 10 a.m. on Dec. 13. Students may return to residence halls after 1 p.m. on Jan. 3. Jan. 8 Last day to drop a course without a fee Dance Company Grupo de Rua 8 p.m., Campbell Hall Arts & Lectures tickets may be purchased by calling

California at Santa Barbara, University of

124

Charge breaking bounds in the Zee model  

E-Print Network [OSTI]

We study the possibility that charge breaking minima occur in the Zee model. We reach very different conclusions from those attained in simpler, two Higgs doublet models, and the reason for this is traced back to the existence of cubic terms in the potential. A scan of the Zee model's parameter space shows that CB is restricted to a narrow region of values of the parameters.

A. Barroso; P. M. Ferreira

2005-10-11T23:59:59.000Z

125

Spontaneous Supersymmetry Breaking Induced by Vacuum Condensates  

E-Print Network [OSTI]

We propose a novel mechanism of spontaneous supersymmetry breaking which relies upon an ubiquitous feature of Quantum Field Theory, vacuum condensates. Such condensates play a crucial r\\^{o}le in many phenomena. Examples include Unruh effect, superconductors, particle mixing, and quantum dissipative systems. We argue that in all these phenomena supersymmetry, when present, is spontaneously broken. Evidence for our conjecture is given for the Wess--Zumino model, that can be considered an approximation to the supersymmetric extensions of the above mentioned systems. The magnitude of the effect is estimated for a recently proposed experimental setup based on an optical lattice.

Antonio Capolupo; Marco Di Mauro

2012-08-29T23:59:59.000Z

126

Cloud Properties Working Group Break Out Session  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmospheric Optical Depth7-1D: Vegetation Proposed New SubstationClean Communities of WesternVailCloisteredPresence of AerosolsBreak

127

Exploring Cartan gravity with dynamical symmetry breaking  

E-Print Network [OSTI]

It has been known for some time that General Relativity can be regarded as a Yang-Mills-type gauge theory in a symmetry broken phase. In this picture the gravity sector is described by an $SO(1,4)$ or $SO(2,3)$ gauge field $A^{a}_{\\phantom{a}b\\mu}$ and Higgs field $V^{a}$ which acts to break the symmetry down to that of the Lorentz group $SO(1,3)$. This symmetry breaking mirrors that of electroweak theory. However, a notable difference is that while the Higgs field $\\Phi$ of electroweak theory is taken as a genuine dynamical field satisfying a Klein-Gordon equation, the gauge independent norm $V^2\\equiv \\eta_{ab}V^{a}V^{b}$ of the Higgs-type field $V^a$ is typically regarded as non-dynamical. Instead, in many treatments $V^a$ does not appear explicitly in the formalism or is required to satisfy $V^2 = \\mathrm{const.} \

H. F. Westman; T. G. Zlosnik

2014-03-24T23:59:59.000Z

128

Chip breaking system for automated machine tool  

DOE Patents [OSTI]

The invention is a rotary selectively directional valve assembly for use in an automated turret lathe for directing a stream of high pressure liquid machining coolant to the interface of a machine tool and workpiece for breaking up ribbon-shaped chips during the formation thereof so as to inhibit scratching or other marring of the machined surfaces by these ribbon-shaped chips. The valve assembly is provided by a manifold arrangement having a plurality of circumferentially spaced apart ports each coupled to a machine tool. The manifold is rotatable with the turret when the turret is positioned for alignment of a machine tool in a machining relationship with the workpiece. The manifold is connected to a non-rotational header having a single passageway therethrough which conveys the high pressure coolant to only the port in the manifold which is in registry with the tool disposed in a working relationship with the workpiece. To position the machine tools the turret is rotated and one of the tools is placed in a material-removing relationship of the workpiece. The passageway in the header and one of the ports in the manifold arrangement are then automatically aligned to supply the machining coolant to the machine tool workpiece interface for breaking up of the chips as well as cooling the tool and workpiece during the machining operation.

Arehart, Theodore A. (Clinton, TN); Carey, Donald O. (Oak Ridge, TN)

1987-01-01T23:59:59.000Z

129

1Ottawa 7/05 Structural Break DetectionStructural Break Detection  

E-Print Network [OSTI]

://www.stat.colostate.edu/~rdavis/lectures) This research supported in part by an IBM faculty award. #12;2Ottawa 7/05 Introduction Setup Examples AR GARCH for structural break estimation Simulation results Applications Simulation results for GARCH and SSM #12;3Ottawa/05 Examples (cont) 2. Segmented GARCH model: where 0 = 1

130

'Jet breaks' and 'missing breaks' in the X-Ray afterglow of Gamma Ray Bursts  

E-Print Network [OSTI]

The X-ray afterglows (AGs) of Gamma-Ray Bursts (GRBs) and X-Ray Flashes (XRFs) have, after the fast decline phase of their prompt emission, a temporal behaviour varying between two extremes. A large fraction of these AGs has a 'canonical' light curve which, after an initial shallow-decay 'plateau' phase, 'breaks smoothly' into a fast power-law decline. Very energetic GRBs, contrariwise, appear not to have a 'break', their AG declines like a power-law from the start of the observations. Breaks and 'missing breaks' are intimately related to the geometry and deceleration of the jets responsible for GRBs. In the frame of the 'cannonball' (CB) model of GRBs and XRFs, we analyze the cited extreme behaviours (canonical and pure power-law) and intermediate cases spanning the observed range of X-ray AG shapes. We show that the entire panoply of X-ray light-curve shapes --measured with Swift and other satellites-- are as anticipated, on very limpid grounds, by the CB model. We test the expected correlations between the...

Dado, Shlomo; De Rújula, Alvaro

2008-01-01T23:59:59.000Z

131

LANL breaks ground on key sediment control project  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Sediment control project LANL breaks ground on key sediment control project Called "grade-control" structures, the approximately 2 million features are up to eight feet high and...

132

Understanding the Electroweak Symmetry Breaking: The Higgs Boson...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Understanding the Electroweak Symmetry Breaking: The Higgs Boson and Beyond (Monday, March 2) DATE: Monday, March 2, 4:10 pm LOCATION: Physics 0003 Understanding the Electroweak...

133

Symmetry Breaking in Graphene Gregory S. Boebinger, National...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Symmetry Breaking in Graphene Gregory S. Boebinger, National High Magnetic Field Laboratory DMR-Award 0654118 DC Field Facility While the laws of physics are often symmetric,...

134

Isospin-Breaking quark condensates in Chiral Perturbation Theory  

E-Print Network [OSTI]

We analyze the isospin-breaking corrections to quark condensates within one-loop SU(2) and SU(3) Chiral Perturbation Theory including $m_u\

A. Gomez Nicola; R. Torres Andres

2011-11-14T23:59:59.000Z

135

Dynamical electroweak symmetry breaking and the top quark  

SciTech Connect (OSTI)

In this talk, I discuss theories of dynamical electroweak symmetry breaking, with emphasis on the implications of a heavy top quark on the weak interaction {rho} parameter.

Chivukula, R.S. [Boston Univ., MA (United States)

1997-01-01T23:59:59.000Z

136

DNA Sequencing apparatus  

DOE Patents [OSTI]

An automated DNA sequencing apparatus having a reactor for providing at least two series of DNA products formed from a single primer and a DNA strand, each DNA product of a series differing in molecular weight and having a chain terminating agent at one end; separating means for separating the DNA products to form a series bands, the intensity of substantially all nearby bands in a different series being different, band reading means for determining the position an This invention was made with government support including a grant from the U.S. Public Health Service, contract number AI-06045. The U.S. government has certain rights in the invention.

Tabor, Stanley (Cambridge, MA); Richardson, Charles C. (Chestnut Hill, MA)

1992-01-01T23:59:59.000Z

137

December 8, 2011 FRONTIER HALL WINTER BREAK CLOSING INFORMATION  

E-Print Network [OSTI]

December 8, 2011 FRONTIER HALL WINTER BREAK CLOSING INFORMATION Frontier Hall will officially close directly before you leave. After 8pm you will no longer be able to access Frontier Hall. Please keep will need during this time. We cannot let you back into Frontier once we have closed for break. If you

Janssen, Michel

138

Resonant Instability in Mountain Waves: Breaking at Subcritical Mountain Heights  

E-Print Network [OSTI]

Resonant Instability in Mountain Waves: Breaking at Subcritical Mountain Heights Kevin Viner1 and breaks subcritical critical Nh/U = 0.5 Nh/U = 0.8 #12;Subcritical Instability: An Example three peaks · Nh/U = 0.6 · U/NL = 0.1 · nonrotating · Time-dependent model initialized with subcritical steady wave

139

Detection and quantitation of single nucleotide polymorphisms, DNA sequence variations, DNA mutations, DNA damage and DNA mismatches  

SciTech Connect (OSTI)

DNA mutation binding proteins alone and as chimeric proteins with nucleases are used with solid supports to detect DNA sequence variations, DNA mutations and single nucleotide polymorphisms. The solid supports may be flow cytometry beads, DNA chips, glass slides or DNA dips sticks. DNA molecules are coupled to solid supports to form DNA-support complexes. Labeled DNA is used with unlabeled DNA mutation binding proteins such at TthMutS to detect DNA sequence variations, DNA mutations and single nucleotide length polymorphisms by binding which gives an increase in signal. Unlabeled DNA is utilized with labeled chimeras to detect DNA sequence variations, DNA mutations and single nucleotide length polymorphisms by nuclease activity of the chimera which gives a decrease in signal.

McCutchen-Maloney, Sandra L. (Pleasanton, CA)

2002-01-01T23:59:59.000Z

140

1SCRA 2006 FIM XIII-PORTUGAL Structural Break Detection for a ClassStructural Break Detection for a Class  

E-Print Network [OSTI]

1000 -4-202 Any breaks in this series? #12;5SCRA 2006 FIM XIII-PORTUGAL Introduction Examples AR GARCH for structural break estimation Simulation results Applications Simulation results for GARCH and SV models #12 (cont) 2. Segmented GARCH model: where 0 = 1

Note: This page contains sample records for the topic "dna double-strand breaks" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


141

1NCAR-IMAGe 2006 Structural Break Detection in Time Series ModelsStructural Break Detection in Time Series Models  

E-Print Network [OSTI]

-202 Any breaks in this series? #12;5NCAR-IMAGe 2006 Introduction Examples AR GARCH Stochastic volatility break estimation Simulation results Applications Simulation results for GARCH and SV models #12;6NCAR-tjptjptjjt tYYY jj GARCH model

142

Creaming and breaking of liquid emulsions: a free boundary problem 1 / 48 Creaming and breaking of liquid  

E-Print Network [OSTI]

, as opposite to the other case called instead a water-in-oil emulsion. #12;Creaming and breaking of liquid emulsions: a free boundary problem 4 / 48 What is an emulsion? Everyday life examples (a) water-in-oil of the model 5 A priori estimates 6 Existence and uniqueness #12;Creaming and breaking of liquid emulsions

Rosso, Fabio

143

Received 12 Apr 2013 | Accepted 30 May 2013 | Published 2 Jul 2013 CRISPR-Cas and restrictionmodification systems  

E-Print Network [OSTI]

-Cas and restriction­modification systems are compatible and increase phage resistance Marie-E`ve Dupuis1,2, Manuela systems exist and at least two of them directly target the incoming DNA: restriction­modification (R plasticity. The most studied phages have double-stranded DNA (dsDNA) genome encapsidated in a protein capsid

Cai, Long

144

Charge symmetry breaking in $\\Lambda$ hypernuclei revisited  

E-Print Network [OSTI]

The large charge symmetry breaking (CSB) implied by the $\\Lambda$ binding energy difference $\\Delta B^{4}_{\\Lambda}(0^+_{\\rm g.s.})\\equiv B_{\\Lambda}(_{\\Lambda}^4$He)$-$$B_{\\Lambda}(_{\\Lambda}^4$H) = 0.35$\\pm$0.06 MeV of the $A=4$ mirror hypernuclei ground states, determined from emulsion studies, has defied theoretical attempts to reproduce it in terms of CSB in hyperon masses and in hyperon-nucleon interactions, including one pion exchange arising from $\\Lambda-\\Sigma^0$ mixing. Using a schematic strong-interaction $\\Lambda N\\leftrightarrow\\Sigma N$ coupling model developed by Akaishi and collaborators for $s$-shell $\\Lambda$ hypernuclei, we revisit the evaluation of CSB in the $A=4$ $\\Lambda$ hypernuclei and extend it to $p$-shell mirror $\\Lambda$ hypernuclei. The model yields values of $\\Delta B^{4}_{\\Lambda} (0^+_{\\rm g.s.})\\sim 0.25$ MeV. Smaller size and mostly negative $p$-shell binding energy differences are calculated for the $A=7-10$ mirror hypernuclei, in rough agreement with the few available dat...

Gal, Avraham

2015-01-01T23:59:59.000Z

145

Wave breaking along the stratospheric polar vortex as seen in ERA-40 data  

E-Print Network [OSTI]

1983), Breaking planetary waves in the stratosphere, Nature,structure of breaking Rossby waves in the polar wintertimecontrol of upward wave flux near the tropopause, Geophys.

Abatzoglou, John T; Magnusdottir, Gudrun

2007-01-01T23:59:59.000Z

146

SciTech Connect: Sphericity and symmetry breaking in the formation...  

Office of Scientific and Technical Information (OSTI)

and symmetry breaking in the formation of FrankKasper phases from one component materials Citation Details In-Document Search Title: Sphericity and symmetry breaking in the...

147

Novel Thermal Break with Simplified Manufacturing for R7 Commercial...  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

(R5 OptiQ-Ultra Thermal Windows). Windows incorporating the foam-based sandwich-type thermal-break would achieve U-Factor of 0.14 Btuhr-ft2-F (R7 or better) with...

148

New Mechanisms of Gauge-Mediated Supersymmetry Breaking  

E-Print Network [OSTI]

We introduce new mechanisms for the communication of supersymmetry breaking via gauge interactions. These models do not require complicated dynamics to induce a nonvanishing F term for a singlet. The first class of models communicates supersymmetry breaking to the visible sector through a ``mediator" field that transforms under both a messenger gauge group of the dynamical supersymmetry breaking sector and the standard model gauge group. This model has distinctive phenomenology; in particular, the scalar superpartners should be heavier by at least an order of magnitude than the gaugino superpartners. The second class of models has phenomenology more similar to the ``standard" messenger sectors. A singlet is incorporated, but the model does not require complicated mechanisms to generate a singlet F term. The role of the singlet is to couple fields from the dynamical symmetry breaking sector to fields transforming under the standard model gauge group. We also mention a potential solution to the $\\mu$ problem.

Lisa Randall

1996-12-19T23:59:59.000Z

149

Synchronous Symmetry Breaking in Neurons with Different Neurite Counts  

E-Print Network [OSTI]

As neurons develop, several immature processes (i.e., neurites) grow out of the cell body. Over time, each neuron breaks symmetry when only one of its neurites grows much longer than the rest, becoming an axon. This symmetry ...

Wissner-Gross, Zachary D.

150

Tackling Epistemological Problems (TEP) A didactical engineering to break through  

E-Print Network [OSTI]

Tackling Epistemological Problems (TEP) A didactical engineering to break through the amazing world, becomes a didactical obstacle for the learning of this geometry. We need to use 3D models and also dynamic

Spagnolo, Filippo

151

Sets of Symmetry Breaking Constraints Barbara M. Smith  

E-Print Network [OSTI]

Sets of Symmetry Breaking Constraints Barbara M. Smith Cork Constraint Computation Centre, University College Cork, Ireland b.m.smith@4c.ucc.ie Abstract [Puget, 2004] has shown that if the symmetry

Smith, Barbara M.

152

1Kaiserslautern 9/05 Structural Break Detection in Time Series ModelsStructural Break Detection in Time Series Models  

E-Print Network [OSTI]

Regressive Modeling 4 pieces, 2.58 seconds. #12;4Kaiserslautern 9/05 Introduction Examples AR GARCH Stochastic for structural break estimation Simulation results Applications Simulation results for GARCH and SSM #12. Segmented GARCH model: where 0 = 1

153

Supramolecular DNA nanotechnology : discrete nanoparticle organization, three-dimensional DNA construction, and molecule templated DNA assembly.  

E-Print Network [OSTI]

??The field of structural DNA nanotechnology utilizes DNA's powerful base-pairing molecular recognition criteria to help solve real challenges facing researchers in material science and nanotechnology,… (more)

Aldaye, Faisal A., 1979-

2008-01-01T23:59:59.000Z

154

Plectoneme tip bubbles: Coupled denaturation and writhing in supercoiled DNA  

E-Print Network [OSTI]

Biological information is not only stored in the digital chemical sequence of double helical DNA, but is also encoded in the mechanical properties of the DNA strands, which can influence biochemical processes involving its readout. For example, loop formation in the Lac operon can regulate the expression of key genes, and DNA supercoiling is closely correlated to rhythmic circardian gene expression in cyanobacteria. Supercoiling is also important for large scale organisation of the genome in both eukaryotic and prokaryotic cells. DNA can respond to torsional stress by writhing to form looped structures called plectonemes, thus transferring energy stored as twist into energy stored in bending. Denaturation bubbles can also relax torsional stress, with the enthalpic cost of breaking bonds being compensated by their ability to absorb undertwist. Here we predict a novel regime where bubbles form at the tips of plectonemes, and study its properties using coarse-grained simulations. These tip bubbles can occur for both positive and negative supercoiling and greatly reduce plectoneme diffusion by a pinning mechanism. They can cause plectonemes to preferentially localise to AT rich regions, because bubbles more easily form there. The tip-bubble regime occurs for supercoiling densities and forces that are typically encountered for DNA in vivo, and may be exploited for biological control of genomic processes.

Christian Matek; Thomas E. Ouldridge; Jonathan P. K. Doye; Ard A. Louis

2014-04-10T23:59:59.000Z

155

Patterning nanocrystals using DNA  

SciTech Connect (OSTI)

One of the goals of nanotechnology is to enable programmed self-assembly of patterns made of various materials with nanometer-sized control. This dissertation describes the results of experiments templating arrangements of gold and semiconductor nanocrystals using 2'-deoxyribonucleic acid (DNA). Previously, simple DNA-templated linear arrangements of two and three nanocrystals structures have been made.[1] Here, we have sought to assemble larger and more complex nanostructures. Gold-DNA conjugates with 50 to 100 bases self-assembled into planned arrangements using strands of DNA containing complementary base sequences. We used two methods to increase the complexity of the arrangements: using branched synthetic doublers within the DNA covalent backbone to create discrete nanocrystal groupings, and incorporating the nanocrystals into a previously developed DNA lattice structure [2][3] that self-assembles from tiles made of DNA double-crossover molecules to create ordered nanoparticle arrays. In the first project, the introduction of a covalently-branched synthetic doubler reagent into the backbone of DNA strands created a branched DNA ''trimer.'' This DNA trimer templated various structures that contained groupings of three and four gold nanoparticles, giving promising, but inconclusive transmission electron microscopy (TEM) results. Due to the presence of a variety of possible structures in the reaction mixtures, and due to the difficulty of isolating the desired structures, the TEM and gel electrophoresis results for larger structures having four particles, and for structures containing both 5 and 10 nm gold nanoparticles were inconclusive. Better results may come from using optical detection methods, or from improved sample preparation. In the second project, we worked toward making two-dimensional ordered arrays of nanocrystals. We replicated and improved upon previous results for making DNA lattices, increasing the size of the lattices to a length greater than 20 {micro}m, and collecting atomic force microscopy (AFM) images up to 30 {micro}m. We found the lattices' requirement of divalent magnesium cations to stabilize Holliday junctions to be incompatible with the stability of charge-stabilized gold nanoparticles used for the experiments here, and gold particles added indiscriminately to the lattice surface through non-specific binding. Redesigning the lattices to avoid magnesium may improve results.

Williams, Shara Carol

2003-09-01T23:59:59.000Z

156

Gel and process for preventing carbon dioxide break through  

SciTech Connect (OSTI)

A process is described for retarding the flow of carbon dioxide in carbon dioxide break-through fingers in a subterranean formation, the process comprising: (a) introducing a gas selected from the group consisting of carbon dioxide and gases containing carbon dioxide into a subterranean deposit containing carbon dioxide break-through fingers; (b) after the carbon dioxide break-through fingers have sorbed a predetermined amount of the gas, stopping the flow of the gas into the subterranean formation, (c) after stopping the flow of the gas into the subterranean formation, introducing an effective amount of a gel-forming composition into the subterranean formation and into the carbon dioxide break-through fingers, the gel-forming composition being operable, when contacting carbon dioxide break-through fingers containing the brine which has absorbed substantial amounts of carbon dioxide to form a gel in the fingers which is operable for retarding the flow of the gas in the finger. The gel-forming composition comprises: i. an aqueous solution comprising a first substance selected from the group consisting of polyvinyl alcohols, polyvinyl alcohol copolymers, and mixtures thereof, and ii. an amount of a second substance selected from the group consisting of aldehydes, aldehyde generating substances, acetals, acetal generating substances, and mixtures thereof.

Sandiford, B.B.; Zillmer, R.C.

1987-06-16T23:59:59.000Z

157

Strandwise translocation of a DNA glycosylase on undamaged DNA  

SciTech Connect (OSTI)

Base excision repair of genotoxic nucleobase lesions in the genome is critically dependent upon the ability of DNA glycosylases to locate rare sites of damage embedded in a vast excess of undamaged DNA, using only thermal energy to fuel the search process. Considerable interest surrounds the question of how DNA glycosylases translocate efficiently along DNA while maintaining their vigilance for target damaged sites. Here, we report the observation of strandwise translocation of 8-oxoguanine DNA glycosylase, MutM, along undamaged DNA. In these complexes, the protein is observed to translocate by one nucleotide on one strand while remaining untranslocated on the complementary strand. We further report that alterations of single base-pairs or a single amino acid substitution (R112A) can induce strandwise translocation. Molecular dynamics simulations confirm that MutM can translocate along DNA in a strandwise fashion. These observations reveal a previously unobserved mode of movement for a DNA-binding protein along the surface of DNA.

Qi, Yan; Nam, Kwangho; Spong, Marie C.; Banerjee, Anirban; Sung, Rou-Jia; Zhang, Michael; Karplus, Martin; Verdine, Gregory L. (Harvard)

2012-05-14T23:59:59.000Z

158

Breaking anchored droplets in a microfluidic Hele-Shaw cell  

E-Print Network [OSTI]

We study microfluidic self digitization in Hele-Shaw cells using pancake droplets anchored to surface tension traps. We show that above a critical flow rate, large anchored droplets break up to form two daughter droplets, one of which remains in the anchor. Below the critical flow velocity for breakup the shape of the anchored drop is given by an elastica equation that depends on the capillary number of the outer fluid. As the velocity crosses the critical value, the equation stops admitting a solution that satisfies the boundary conditions; the drop breaks up in spite of the neck still having finite width. A similar breaking event also takes place between the holes of an array of anchors, which we use to produce a 2D array of stationary drops in situ.

Amselem, Gabriel; Gallaire, François; Baroud, Charles N

2015-01-01T23:59:59.000Z

159

Application of break preclusion concept in German nuclear power plants  

SciTech Connect (OSTI)

The break preclusion concept is based on {open_quotes}KTA rules{close_quotes}, {open_quotes}RSK guidelines{close_quotes} and {open_quotes}Rahmenspeziflkation Basissicherheit{close_quotes}. These fundamental rules containing for example requirements on material, design, calculation, manufacturing and testing procedures are explained and the technical realisation is shown by means of examples. The proof of the quality of these piping systems can be executed by means of fracture mechanics calculations by showing that in every case the leakage monitoring system already detect cracks which are clearly smaller than the critical crack. Thus the leak before break behavior and the break preclusion concept is implicitly affirmed. In order to further diminish conservativities in the fracture mechanics procedures, specific research projects are executed which are explained in this contribution.

Roos, E. [Energie-Versorgung Schwaben AG, Stuttgart (Germany); Maier, V. [Bayernwerk AG, Muenchen (Germany); Nagel, G. [PraussenElektra AG, Hannover (Germany)] [and others

1997-04-01T23:59:59.000Z

160

Lorentz symmetry breaking as a quantum field theory regulator  

SciTech Connect (OSTI)

Perturbative expansions of quantum field theories typically lead to ultraviolet (short-distance) divergences requiring regularization and renormalization. Many different regularization techniques have been developed over the years, but most regularizations require severe mutilation of the logical foundations of the theory. In contrast, breaking Lorentz invariance, while it is certainly a radical step, at least does not damage the logical foundations of the theory. I shall explore the features of a Lorentz symmetry breaking regulator in a simple polynomial scalar field theory and discuss its implications. In particular, I shall quantify just 'how much' Lorentz symmetry breaking is required to fully regulate the quantum theory and render it finite. This scalar field theory provides a simple way of understanding many of the key features of Horava's recent article [Phys. Rev. D 79, 084008 (2009)] on 3+1 dimensional quantum gravity.

Visser, Matt [School of Mathematics, Statistics, and Operations Research, Victoria University of Wellington, Wellington 6140 (New Zealand)

2009-07-15T23:59:59.000Z

Note: This page contains sample records for the topic "dna double-strand breaks" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


161

Modulated Tool-Path (MTP) Chip Breaking System  

SciTech Connect (OSTI)

The Modulated Tool-Path (MTP) Chip Breaking System produces user-selectable chip lengths and workpiece finishes and is compatible with any material, workpiece shape, and depth of cut. The MTP chip breaking system consistently creates the desired size of chips regardless of workpiece size, shape, or material, and the machine operator does not need to make any adjustments during the machining operation. The system's programmer configures the part program that commands the machine tool to move in a specific fashion to deliver the desired part size, shape, chip length, and workpiece surface finish. The MTP chip breaking system helps manufacturers avoid the detrimental effects of continuous chips, including expensive repair costs, delivery delays, and hazards to personnel.

Graham, K. B.

2010-04-01T23:59:59.000Z

162

Charge Symmetry Breaking and Parity Violating Electron-Proton Scattering  

E-Print Network [OSTI]

Charge symmetry breaking contributions to the proton's neutral weak form factors must be understood in order for future measurements of parity violating electron-proton scattering to be definitively interpreted as evidence of proton strangeness. We calculate these charge symmetry breaking form factor contributions using chiral perturbation theory with resonance saturation estimates for unknown low-energy constants. The uncertainty of the leading-order resonance saturation estimates is reduced by incorporating nuclear physics constraints. Higher-order contributions are investigated through phenomenological vertex form factors. We predict that charge symmetry breaking form factor contributions are an order of magnitude larger than expected from naive dimensional analysis but are still an order of magnitude smaller than current experimental bounds on proton strangeness. This is consistent with previous calculations using chiral perturbation theory with resonance saturation.

Michael Wagman; Gerald A. Miller

2014-12-16T23:59:59.000Z

163

Comparing Measured Fluorocarbon Leader Breaking Strength with Manufacturer Claims  

E-Print Network [OSTI]

The experiment reported in this article addresses manufacturer claims of fluorocarbon leader material strength versus experimental tests of leaders strength. Breaking strength of fishing line is the most common specification when marketing fishing line. In this study, eight leaders rated near 15 pounds by their manufacturers were tested. Each leader was tested with a knot in the line and without a knot in the line. The strongest leader tested without a knot was Cabela's Seaguar fluorocarbon and the weakest leader tested without a knot was Cabela's Premier leader. The highest strength of leaders with a knot was the Ande Monofilament Fluorocarbon and the lowest breaking strength of leaders with a knot was the Seaguar Grand Max Fluorocarbon. Few published studies actually test the breaking strength of a leader to determine the accuracy of manufacturers' claims. Tensile strengths are also reported.

Haight, Christine; McQueeney, Kathleen; Courtney, Ya'el

2012-01-01T23:59:59.000Z

164

Coarse-graining DNA for simulations of DNA nanotechnology  

E-Print Network [OSTI]

To simulate long time and length scale processes involving DNA it is necessary to use a coarse-grained description. Here we provide an overview of different approaches to such coarse graining, focussing on those at the nucleotide level that allow the self-assembly processes associated with DNA nanotechnology to be studied. OxDNA, our recently-developed coarse-grained DNA model, is particularly suited to this task, and has opened up this field to systematic study by simulations. We illustrate some of the range of DNA nanotechnology systems to which the model is being applied, as well as the insights it can provide into fundamental biophysical properties of DNA.

Doye, Jonathan P K; Louis, Ard A; Romano, Flavio; Sulc, Petr; Matek, Christian; Snodin, Benedict E K; Rovigatti, Lorenzo; Schreck, John S; Harrison, Ryan M; Smith, William P J

2013-01-01T23:59:59.000Z

165

Cis, Isotactic Selective ROMP of Norbornenes Fused with N-Arylpyrrolidines. Double Stranded Polynorbornene-Based Ladderphanes with Z-Double Bonds  

E-Print Network [OSTI]

Upon treatment with a molybdenum-carbene with bidentate substituted biaryl-o,o?-diphenoxide ligand 12, norbornene fused with N-aryl-endo-pyrrolidine 9a undergoes ROMP to give exclusively polynorbornene with cis, ...

Zhu, Lei

166

Monosporascus root rot/vine decline: a study of double-stranded (DS) RNA and its role in the pathogenesis of Monosporascus cannonballus on muskmelon  

E-Print Network [OSTI]

dodecyl sulfate (SDS), and 10 mg of carborundum (Sigma Chemical Co. , St. Louis, MO) for 1 min and incubated for 30 min at 37'C to allow for degradation of host RNAs. Nucleic acids were extracted with an equal volume of TE saturated phenol...

Batten, Jeffrey Samuel

1997-01-01T23:59:59.000Z

167

Margins in high temperature leak-before-break assessments  

SciTech Connect (OSTI)

Developments in the defect assessment procedure R6 to include high-temperature mechanisms in Leak-before-Break arguments are described. In particular, the effect of creep on the time available to detect a leak and on the crack opening area, and hence leak rate, is discussed. The competing influence of these two effects is emphasized by an example. The application to Leak-before-Break of the time-dependent failure assessment diagram approach for high temperature defect assessment is then outlined. The approach is shown to be of use in assessing the erosion of margins by creep.

Budden, P.J.; Hooton, D.G.

1997-04-01T23:59:59.000Z

168

Charge and CP symmetry breaking in two Higgs doublet models  

E-Print Network [OSTI]

We show that, for the most generic model with two Higgs doublets possessing a minimum that preserves the $U(1)_{em}$ symmetry, charge breaking (CB) cannot occur. If CB does not occur, the potential could have two different minima, and there is in principle no general argument to show which one is the deepest. The depth of the potential at a stationary point that breaks CB or CP, relative to the $U(1)_{em}$ preserving minimum, is proportional to the squared mass of the charged or pseudoscalar Higgs, respectively.

A. Barroso; P. M. Ferreira; R. Santos

2005-11-07T23:59:59.000Z

169

Breaking the Fuel Cell Cost Barrier | Department of Energy  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE:YearRound-Up fromDepartmentTie Ltd: ScopeDepartment1, DOE/SC-0095 Breakng theBreaking the Fuel

170

DNA polymerase having modified nucleotide binding site for DNA sequencing  

DOE Patents [OSTI]

A modified gene encoding a modified DNA polymerase is disclosed. The modified polymerase incorporates dideoxynucleotides at least 20-fold better compared to the corresponding deoxynucleotides as compared with the corresponding naturally-occurring DNA polymerase. 6 figs.

Tabor, S.; Richardson, C.

1997-03-25T23:59:59.000Z

171

Towards breaking temperature equilibrium in multi-component Eulerian schemes  

SciTech Connect (OSTI)

We investigate the effects ofthermal equilibrium on hydrodynamic flows and describe models for breaking the assumption ofa single temperature for a mixture of components in a cell. A computational study comparing pressure-temperature equilibrium simulations of two dimensional implosions with explicit front tracking is described as well as implementation and J-D calculations for non-equilibrium temperature methods.

Grove, John W [Los Alamos National Laboratory; Masser, Thomas [Los Alamos National Laboratory

2009-01-01T23:59:59.000Z

172

Vacuum condensates, flavor mixing and spontaneous supersymmetry breaking  

E-Print Network [OSTI]

Spontaneous supersymmetry (SUSY) breaking is revealed in all phenomena in which vacuum condensates are physically relevant. The dynamical breakdown of SUSY is generated by the condensates themselves, which lift the zero point energy. Evidence is presented in the case of the Wess-Zuimino model, and the flavor mixing case is treated in detail.

Antonio Capolupo; Marco Di Mauro

2013-01-05T23:59:59.000Z

173

BREAKING WITH OR BUILDING ON THE PAST? REFORMING IRISH PUBLIC  

E-Print Network [OSTI]

of the application of new public management styles to Ireland. Particular attention is paid to the public serviceBREAKING WITH OR BUILDING ON THE PAST? REFORMING IRISH PUBLIC ADMINISTRATION: 1958-2008 Niamh? REFORMING IRISH PUBLIC ADMINISTRATION: 1958-2008 Niamh Hardiman and Muiris MacCarthaigh Working Papers

174

Breaking the Hierarchy: Distributed Control & Economic Optimality in Microgrids  

E-Print Network [OSTI]

Breaking the Hierarchy: Distributed Control & Economic Optimality in Microgrids Florian D¨orfler. A preliminary version of part of the results in this document has been presented in [1]. F. D¨orfler is with the Department of Electrical Engineering, Uni- versity of California Los Angeles. Email: dorfler@seas.ucla.edu. J

Bullo, Francesco

175

Mechanisms of cellular symmetry breaking in S. cerevisiae  

E-Print Network [OSTI]

………………… … … … … … … … … … … … … … … … … … … … … … … … … … … … … … … … … …..1 Chapter 2: Non ?uniform membrane diffusion enables steady?state cell polarization via vesicular trafficking………………………… … … … … … … … … … … … … … … … … … … … … … … … … … … … … … … … …..34 Chapter 3: Independence of symmetry breaking on... Bem1?mediated autocatalytic activation of Cdc42……………………… … … … … … … … … … … … … … … … … … … … … … … … … … … … … … … … … … … … … … … … ….88 Chapter 4: Preliminary data on Cdc42 microdomains in the presence of Rdi1 feedback………..135 Chapter 5...

Smith, Sarah Ellen

2013-12-31T23:59:59.000Z

176

EasyVoice: Breaking barriers for people with voice disabilities  

E-Print Network [OSTI]

disabilities. With the advances in computing power, new applications have been developed for helping peopleEasyVoice: Breaking barriers for people with voice disabilities Paulo A. Condado and Fernando G,flobo}@ualg.pt Abstract. Text-to-speech technology has been broadly used to help people with voice disabilities

Condado, Paulo

177

Structural Testing at the Micro and Nano Scales: Breaking  

E-Print Network [OSTI]

Structural Testing at the Micro and Nano Scales: Breaking Invisible Specimens With Zero Force). "Nano measurements with micro devices: mechanical properties of hydrated collagen fibrils," J. of the R tangential slots · Fuel swirls in the spin chamber and exits through the orifice in a hollow conical spray

Ballarini, Roberto

178

How does a thermal binary crystal break under shear?  

E-Print Network [OSTI]

When exposed to strong shearing, the particles in a crystal will rearrange and ultimately, the crystal will break by forming large nonaffine defects. Even for the initial stage of this process, only little effort has been devoted to the understanding of the breaking process on the scale of the individual particle size for thermalized mixed crystals. Here, we explore the shear-induced breaking for an equimolar two-dimensional binary model crystal with a high interaction asymmetry between the two different species such that the initial crystal has an intersecting square sublattice of the two constituents. Using Brownian dynamics computer simulations, we show that the combination of shear and thermal fluctuations leads to a characteristic hierarchical breaking scenario where initially, the more strongly coupled particles are thermally distorted, paving the way for the weakly coupled particles to escape from their cage. This in turn leads to mobile defects which may finally merge, proliferating a cascade of defects, which triggers the final breakage of the crystal. This scenario is in marked contrast to the breakage of one-component crystals close to melting. Moreover, we explore the orientational dependence of the initial shear direction relative to the crystal orientation and compare this to the usual melting scenario without shear. Our results are verifiable in real-space experiments of superparamagnetic colloidal mixtures at a pending air-water interface in an external magnetic field where the shear can be induced by an external laser field.

Tobias Horn; Hartmut Löwen

2014-11-21T23:59:59.000Z

179

Building Cathedrals and Breaking down Reinforced Concrete Walls  

E-Print Network [OSTI]

Building Cathedrals and Breaking down Reinforced Concrete Walls Michel Brou´e Institut Henri distinction between great mathematicians Concrete walls breakers Michel Brou´e (Institut Henri Poincar´e) John Concrete walls breakers Cathedrals builders Michel Brou´e (Institut Henri Poincar´e) John Thompson

Broué, Michel - Institut de Mathématiques de Jussieu, Université Paris 7

180

DNA Structural Nanotechnology Duke University  

E-Print Network [OSTI]

DNA Structural Nanotechnology John Reif Duke University Graduate Students: Harish Chandran&Caltech Tube Lattices #12;Ned Seeman New York University, USA Ned Seeman: Father of DNA Nanotechnology His Initial Ideas & Motivation for DNA Nanotechnology #12;Cube Chen & Seeman, Nature350:631 (1991) Truncated

Reif, John H.

Note: This page contains sample records for the topic "dna double-strand breaks" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


181

1Banff 6/06 Structural Break Detection in Time Series ModelsStructural Break Detection in Time Series Models  

E-Print Network [OSTI]

breaks in this series? #12;5Banff 6/06 Introduction Examples AR GARCH Stochastic volatility State space Simulation results Applications Simulation results for GARCH and SV models #12;6Banff 6/06 Examples 1 ),,( 1 jjpj K #12;7Banff 6/06 Examples (cont) 2. Segmented GARCH model: where 0 = 1

182

FastFEM: Breaking Wave Impact on Ships Wave breaking and wave impact on maritime structures are difficult to model  

E-Print Network [OSTI]

with fine-scale dynamic air-water interfaces need to be modelled efficiently, including their interactions on fast ships. Faster ships have become more important in recent years. These include pilot ships, supply. Improved understanding of ship motion in heavy breaking seas is a key part of the larger project

Wirosoetisno, Djoko

183

Does quantum entanglement in DNA synchronize the catalytic centers of type II restriction endonucleases?  

E-Print Network [OSTI]

Several living systems have been examined for their apparent optimization of structure and function for quantum behavior at biological length scales. Orthodox type II endonucleases, the largest class of restriction enzymes, recognize four-to-eight base pair sequences of palindromic DNA, cut both strands symmetrically, and act without an external metabolite such as ATP. While it is known that these enzymes induce strand breaks by attacking phosphodiester bonds, what remains unclear is the mechanism by which cutting occurs in concert at the catalytic centers. Previous studies indicate the primacy of intimate DNA contacts made by the specifically bound enzyme in coordinating the two synchronized cuts. We propose that collective electronic behavior in the DNA helix generates coherent oscillations, quantized through boundary conditions imposed by the endonuclease, that provide the energy required to break two phosphodiester bonds. Such quanta may be preserved in the presence of thermal noise and electromagnetic interference through decoherence shielding, the specific complex's exclusion of water and ions surrounding the helix. Clamping energy imparted by the enzyme decoherence shield is comparable with zero-point modes of the dipole-dipole oscillations in the DNA recognition sequence. The palindromic mirror symmetry of this sequence should conserve parity during the process. Experimental data corroborate that symmetric bond-breaking ceases when the symmetry of the endonuclease complex is violated, or when environmental parameters are perturbed far from biological optima. Persistent correlation between states in DNA sequence across spatial separations of any length--a characteristic signature of quantum entanglement--may be explained by such a physical mechanism.

P. Kurian; G. Dunston; J. Lindesay

2014-03-21T23:59:59.000Z

184

Experimental investigation of small-scale breaking waves : flow visualization across the air-water interface  

E-Print Network [OSTI]

The dynamics of breaking waves significantly affect air-sea fluxes of heat, momentum, mass and energy across the ocean interface. Breaking waves also contribute considerable loading to offshore and coastal structures, and ...

McDonald, Angus Kai

2005-01-01T23:59:59.000Z

185

Navier-Stokes simulations of steep breaking water waves with a coupled air-water interface  

E-Print Network [OSTI]

Wave breaking on the ocean surface significantly facilitates the transfer of mass, momentum, heat and energy across the air-sea interface. In the context of the near field flow about a surface ship, the breaking bow wave ...

Hendrickson, Kelli L

2005-01-01T23:59:59.000Z

186

Quantitative imaging of the air-water flow fields formed by unsteady breaking waves  

E-Print Network [OSTI]

An experimental method for simultaneously measuring the velocity fields on the air and water side of unsteady breaking waves is presented. The method is applied to breaking waves to investigate the physics of the air and ...

Belden, Jesse (Jesse Levi)

2009-01-01T23:59:59.000Z

187

U.S. Department of Energy Breaks Ground on State-of-the-Art Wind...  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

U.S. Department of Energy Breaks Ground on State-of-the-Art Wind Turbine Test Facility U.S. Department of Energy Breaks Ground on State-of-the-Art Wind Turbine Test Facility...

188

Three dimensional effects in analysis of PWR steam line break accident  

E-Print Network [OSTI]

A steam line break accident is one of the possible severe abnormal transients in a pressurized water reactor. It is required to present an analysis of a steam line break accident in the Final Safety Analysis Report (FSAR) ...

Tsai, Chon-Kwo

189

Fleet DNA (Presentation)  

SciTech Connect (OSTI)

The Fleet DNA project objectives include capturing and quantifying drive cycle and technology variation for the multitude of medium- and heavy-duty vocations; providing a common data storage warehouse for medium- and heavy-duty vehicle fleet data across DOE activities and laboratories; and integrating existing DOE tools, models, and analyses to provide data-driven decision making capabilities. Fleet DNA advantages include: for Government - providing in-use data for standard drive cycle development, R&D, tech targets, and rule making; for OEMs - real-world usage datasets provide concrete examples of customer use profiles; for fleets - vocational datasets help illustrate how to maximize return on technology investments; for Funding Agencies - ways are revealed to optimize the impact of financial incentive offers; and for researchers -a data source is provided for modeling and simulation.

Walkokwicz, K.; Duran, A.

2014-06-01T23:59:59.000Z

190

Chiral Mesophases of DNA  

E-Print Network [OSTI]

In the hexagonal columnar phase of chiral polymers a bias towards cholesteric twist competes with braiding along an average direction. When the chirality is strong, topological defects proliferate, leading to either a tilt grain boundary phase or a new ``moire state'' with twisted bond order. This moire phase can melt leading to a new phase: the chiral hexatic. I will discuss some recent experimental results from the NIH on DNA liquid crystals in the context of these theories.

Randall D. Kamien

1998-12-08T23:59:59.000Z

191

DNA waves and water  

E-Print Network [OSTI]

Some bacterial and viral DNA sequences have been found to induce low frequency electromagnetic waves in high aqueous dilutions. This phenomenon appears to be triggered by the ambient electromagnetic background of very low frequency. We discuss this phenomenon in the framework of quantum field theory. A scheme able to account for the observations is proposed. The reported phenomenon could allow to develop highly sensitive detection systems for chronic bacterial and viral infections.

L. Montagnier; J. Aissa; E. Del Giudice; C. Lavallee; A. Tedeschi; G. Vitiello

2010-12-23T23:59:59.000Z

192

Density driven symmetry breaking and Butterfly effect in holographic superconductors  

E-Print Network [OSTI]

We study the density driven symmetry breaking in holographic superconductors by considering the positive mass squared case. We show that even for the positive $m^2$, a scalar condensation still forms, provided the chemical potential is high enough. As $m^2$ increases, the phase space folds due to the non-linearity of the equations of motion, and two nearby points in the phase space can represent symmetry breaking and preserving configurations respectively. The phase space defined by the set of initial conditions of field variables at the horizon undergoes a non-linear radial evolution to result in the phase space folding, a characteristic phenomenon in a non-linear system. We then calculate the specific heat, which characterizes superconductors and has been measured in experiments.We observe a discontinuity in the specific heat at the transition point and compare our results with experimentally observed numbers. The electrical conductivity for various $m^2$ is also calculated.

Youngman Kim; Yumi Ko; Sang-Jin Sin

2009-12-01T23:59:59.000Z

193

The Environmental Impact of Lyman-break Galaxies  

E-Print Network [OSTI]

We perform cosmological simulations of galaxies forming at z=3 using the hydrodynamics grid code, Enzo. By selecting the largest galaxies in the volume to correspond to Lyman-break galaxies, we construct observational spectra of the HI flux distribution around these objects, as well as column densities of CIV and OVI throughout a refined region. We successfully reproduce the most recent observations of the mean HI flux in the close vicinity of Lyman-break galaxies but see no evidence for the proximity effect in earlier observations. While our galaxies do return metals to the IGM, their quantity and volume appears to be somewhat less than observed. We conclude that either we do not adequately resolve galactic winds, or that at least some of the intergalactic metal enrichment is by early epoch objects whose mass is smaller than our minimum resolved halo mass.

Elizabeth J. Tasker; Greg L. Bryan

2006-04-11T23:59:59.000Z

194

A Gravity Dual of Metastable Dynamical Supersymmetry Breaking  

SciTech Connect (OSTI)

Metastable, supersymmetry-breaking configurations can be created in flux geometries by placing antibranes in warped throats. Via gauge/gravity duality, such configurations should have an interpretation as supersymmetry-breaking states in the dual field theory. In this paper, we perturbatively determine the asymptotic supergravity solutions corresponding to D3-brane probes placed at the tip of the cascading warped deformed conifold geometry, which is dual to an SU(N+M) x SU(N) gauge theory. The backreaction of the antibranes has the effect of introducing imaginary anti-self-dual flux, squashing the compact part of the space and forcing the dilaton to run. Using the generalization of holographic renormalization to cascading geometries, we determine the expectation values of operators in the dual field theory in terms of the asymptotic values of the supergravity fields.

DeWolfe, Oliver; /Colorado U.; Kachru, Shamit; Mulligan, Michael; /Stanford U., Phys. Dept. /SLAC

2008-02-04T23:59:59.000Z

195

Landscape of Supersymmetry Breaking Vacua in Geometrically Realized Gauge Theories  

E-Print Network [OSTI]

We study vacuum structure of N=1 supersymmetric quiver gauge theories which can be realized geometrically by D brane probes wrapping cycles of local Calabi-Yau three-folds. In particular, we show that the A_2 quiver theory with gauge group U(N_1) \\times U(N_2) with N_1 / 2 landscape of inequivalent meta-stable vacua where supersymmetry is dynamically broken and all the moduli are stabilized. Each vacuum has distinct unbroken gauge symmetry. B-terms generated by the supersymmetry breaking give rise to gaugino masses at one-loop, and we are left with the bosonic pure Yang-Mills theory in the infrared. We also identify the supersymmetric vacua in this model using their infrared free descriptions and show that the decay rates of the supersymmetry breaking vacua into the supersymmetric vacua can be made parametrically small.

Hirosi Ooguri; Yutaka Ookouchi

2006-07-07T23:59:59.000Z

196

GENTLE IONISATION AND VIOLENT BREAK-UP OF MOLECULES USING SOFT X-RAYS  

E-Print Network [OSTI]

GENTLE IONISATION AND VIOLENT BREAK-UP OF MOLECULES USING SOFT X-RAYS Bhas Bapat PRL, Ahmedabad RRCAT Indore, June 2012 BHAS BAPAT (PRL, AHMEDABAD) BREAK-UP OF MOLECULES USING SOFT X-RAYS RRCAT INDORE INVESTIGATED RECENT RESULTS BHAS BAPAT (PRL, AHMEDABAD) BREAK-UP OF MOLECULES USING SOFT X-RAYS RRCAT INDORE

Bapat, Bhas

197

Modeling suggests that oblique extension facilitates rifting and continental break-up  

E-Print Network [OSTI]

Modeling suggests that oblique extension facilitates rifting and continental break-up Sascha Brune; accepted 5 June 2012; published 2 August 2012. [1] In many cases the initial stage of continental break-up was and is associated with oblique rifting. That includes break-up in the Southern and Equatorial Atlantic, separation

Kaus, Boris

198

Gauge/gravity Duality and MetastableDynamical Supersymmetry Breaking  

SciTech Connect (OSTI)

We engineer a class of quiver gauge theories with several interesting features by studying D-branes at a simple Calabi-Yau singularity. At weak 't Hooft coupling we argue using field theory techniques that these theories admit both supersymmetric vacua and meta-stable non-supersymmetric vacua, though the arguments indicating the existence of the supersymmetry breaking states are not decisive. At strong 't Hooft coupling we find simple candidate gravity dual descriptions for both sets of vacua.

Argurio, Riccardo; Bertolini, Matteo; Franco, Sebastian; Kachru, Shamit

2006-10-24T23:59:59.000Z

199

FLASH predictions of the MB-2 steam line break tests  

SciTech Connect (OSTI)

If a main steam line from a pressurized water reactor (PWR) steam generator were to rupture, the effect would be a depressurization of the secondary side and a consequential overcooling transient on the primary side. Analyses must accurately predict the effects of the rapid cooldown of the reactor vessel coolant on positive nuclear-kinetic reactivity feedback to the core plus thermal shock to the reactor vessel and other primary system components. Many early studies of the steam line break (SLB) transient made extremely conservative assumptions to maximize the primary to secondary heat transfer which in turn maximized the reactor vessel cooldown rate. Among the more significant of these assumptions was that flow from the break was pure steam and that the tube bundle remained covered until the secondary mass inventory was significantly reduced. The Model F commercial PWR steam generator testing performed in the Model Boiler No. 2 (MB-2) facility located at the Westinghouse Engineering Test Facility in Tampa, Florida provided data to better qualify the actual variation in these key parameters. A conclusion of this analysis is that the MB-2 steam line break data base is accurate and of sufficient detail to provide a valuable basis for making comparisons relative to code predictions. Results obtained using the FLASH transient safety analysis code were found to be in excellent agreement with the data.

Lincoln, F.W.; Coffield, R.D.; Johnson, E.G.

1992-12-31T23:59:59.000Z

200

Nuclear apoJ: A low dose radiation inducible regulator of cell death. Final report for period September 15, 1998 - September 14, 2001  

SciTech Connect (OSTI)

This project was based on preliminary data that was published by Dr. Boothman (Yang et al. 2000) which indicated a strong induction of apoJ gene expression, increased secretion of the protein, and accumulation of an apparently somewhat different form of the apoJ protein in the nucleus of MCF-7 breast carcinoma cells undergoing response to DNA damage. A clone expressing apoJ protein was isolated that was capable of interacting with Ku80, a component of the double strand break repair complex that is essential for the successful repair of rearranging immunoglobulin and T-cell receptor genes as evidenced by failure to produce mature B and T cells in the absence of Ku70. ApoJ clones isolated and characterized by Dr. Boothman bound strongly to a Ku-70 ''bait'' protein. Over-expression of these same clones in a cell line was capable of killing the cell. ApoJ is very strongly induced in many instances of programmed cell death and has been proposed repeatedly to play some sort of effector role in the process. Our principle hypothesis for this study was that the strong induction of the apoJ gene and the particular expression of a nuclear form of the protein was potentially a causal factor in the decision point made by the cell as it attempts to repair double-strand breakage based DNA damage. The hypothesis was that if sufficiently high damage occurred, it would be deleterious to maintain the cell's viability through continued DNA repair. One method to inhibit DNA repair might be by inhibiting proteins such as Ku-70 that are necessary for double-strand break repair. If apoJ does play a critical role in tipping the decision balance over to cell death, we reasoned that deficiency of apoJ would cause increased accumulation of cells with DNA damage and that this might decrease cell death in response to DNA damage and increase tumor occurrence rates. To test this hypothesis and its potential implications, we exposed wildtype and apoJ deficient animals that we constructed through gene targeting to increasing levels of ionizing radiation from a Cesium source. Data gathered under the support of this grant application initially indicated that apoJ deficient animals were more resistant to radiation, but as we accumulated more and more data points and covered a tighter exposure range, the genotype-based differences became insignificant. However, the possibility existed that because mortality based radiation-resistance could be attributable to mechanism for which nuclear apoJ was not rate determining, we maintained a very large of colony of apoJ knockout and wildtype animals in both the C57/B16 and Cv129 strain backgrounds that were exposed to sub-lethal levels of ionizing radiation to monitor for the occurrence of tumors. These animals were allowed to fully recover and age normally in either germ free or normal animal housing. Our results demonstrated no significant differences between wildtype and apoJ knockout animals over a period that extended up to 30 months for individual animals. We recorded similar weight gain, a relatively low mortality rate, and a similar mixture and rate of sarcoma and adenocarcinomas after surviving the initial ionizing radiation exposures. Thus we conclude that apoJ gene function, which was totally eliminated by our gene targeting, did not influence radiation sensitivity or serve as a tumor suppressor in response to DNA damage.

Aronow, Bruce J.

2002-04-19T23:59:59.000Z

Note: This page contains sample records for the topic "dna double-strand breaks" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


201

Vet. Res. (2009) 40:11 www.vetres.org DOI: 10.1051/vetres:2008049  

E-Print Network [OSTI]

Camus-Bouclainville1,2* 1 INRA, UMR 1225, F-31076 Toulouse, France 2 Université de Toulouse ; ENVT ; UMR in death within two weeks [29]. * Corresponding author: c.camus@envt.fr MYXV has a double-stranded DNA

Paris-Sud XI, Université de

202

Gene Assembly from Chip-Synthesized Oligonucleotides  

E-Print Network [OSTI]

molecular biology laboratory. The major stages of our synthesis pipeline are computational design, chip of the constructs. The key principle is that well-designed primers can amplify a desired subset of oligonucleotides synthesis of long double-stranded DNA constructs has a myriad of applications in biology and biological

Church, George M.

203

Virus Research 140 (2009) 138146 Contents lists available at ScienceDirect  

E-Print Network [OSTI]

homepage: www.elsevier.com/locate/virusres The A312L 5 -UTR of Chlorella virus PBCV-1 is a translational Phycodnaviridae Chlorella viruses A312L gene Arabidopsis thaliana Agrobacterium-mediated transformation a b s t r a c t PBCV-1 (Paramecium bursaria Chlorella virus) is a large double stranded DNA virus

Graves, Michael V.

204

The Polymerase Chain Reaction and Branching Processes Fengzhu Sun  

E-Print Network [OSTI]

The Polymerase Chain Reaction and Branching Processes Fengzhu Sun Department of Mathematics, DRB is studied. We also study the distribution of the Hamming distance between two randomly chosen sequences long. The double-stranded DNA molecules are heated to near boiling temperature so that the double

Sun, Fengzhu - Sun, Fengzhu

205

A molecular dynamics simulation of DNA damage induction by ionizing radiation  

E-Print Network [OSTI]

We present a multi-scale simulation of early stage of DNA damages by the indirect action of hydroxyl ($^\\bullet$OH) free radicals generated by electrons and protons. The computational method comprises of interfacing the Geant4-DNA Monte Carlo with the ReaxFF molecular dynamics software. A clustering method was employed to map the coordinates of $^\\bullet$OH-radicals extracted from the ionization track-structures onto nano-meter simulation voxels filled with DNA and water molecules. The molecular dynamics simulation provides the time evolution and chemical reactions in individual simulation voxels as well as the energy-landscape accounted for the DNA-$^\\bullet$OH chemical reaction that is essential for the first principle enumeration of hydrogen abstractions, chemical bond breaks, and DNA-lesions induced by collection of ions in clusters less than the critical dimension which is approximately 2-3 \\AA. We show that the formation of broken bonds leads to DNA base and backbone damages that collectively propagate ...

Abolfath, Ramin M; Chen, Zhe J; Nath, Ravinder

2013-01-01T23:59:59.000Z

206

Cadmium sulfate and CdTe-quantum dots alter DNA repair in zebrafish (Danio rerio) liver cells  

SciTech Connect (OSTI)

Increasing use of quantum dots (QDs) makes it necessary to evaluate their toxicological impacts on aquatic organisms, since their contamination of surface water is inevitable. This study compares the genotoxic effects of ionic Cd versus CdTe nanocrystals in zebrafish hepatocytes. After 24 h of CdSO{sub 4} or CdTe QD exposure, zebrafish liver (ZFL) cells showed a decreased number of viable cells, an accumulation of Cd, an increased formation of reactive oxygen species (ROS), and an induction of DNA strand breaks. Measured levels of stress defense and DNA repair genes were elevated in both cases. However, removal of bulky DNA adducts by nucleotide excision repair (NER) was inhibited with CdSO{sub 4} but not with CdTe QDs. The adverse effects caused by acute exposure of CdTe QDs might be mediated through differing mechanisms than those resulting from ionic cadmium toxicity, and studying the effects of metallic components may be not enough to explain QD toxicities in aquatic organisms. - Highlights: • Both CdSO{sub 4} and CdTe QDs lead to cell death and Cd accumulation. • Both CdSO{sub 4} and CdTe QDs induce cellular ROS generation and DNA strand breaks. • Both CdSO{sub 4} and CdTe QDs induce the expressions of stress defense and DNA repair genes. • NER repair capacity was inhibited with CdSO{sub 4} but not with CdTe QDs.

Tang, Song; Cai, Qingsong [The Institute of Environmental and Human Health, Texas Tech University, Lubbock, TX 79416 (United States); Chibli, Hicham [Department of Biomedical Engineering, McGill University, Montréal, QC H3A 2B4 (Canada); Allagadda, Vinay [The Institute of Environmental and Human Health, Texas Tech University, Lubbock, TX 79416 (United States); Nadeau, Jay L. [Department of Biomedical Engineering, McGill University, Montréal, QC H3A 2B4 (Canada); Mayer, Gregory D., E-mail: greg.mayer@ttu.edu [The Institute of Environmental and Human Health, Texas Tech University, Lubbock, TX 79416 (United States)

2013-10-15T23:59:59.000Z

207

Assessing the Risk of Mercury in Drinking Water after UV Lamp Breaks Page 1 Assessing the Risk of Mercury in Drinking Water after UV Lamp Breaks  

E-Print Network [OSTI]

Assessing the Risk of Mercury in Drinking Water after UV Lamp Breaks Page 1 Assessing the Risk of Mercury in Drinking Water after UV Lamp Breaks Heidi Borchers University of New Hampshire, Environmental Ultraviolet (UV) lamps generate ultraviolet light through the vaporization of elemental mercury, by using

208

A Model for Structure and Thermodynamics of ssDNA and dsDNA Near...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Structure and Thermodynamics of ssDNA and dsDNA Near a Surface:A Coarse Grained Approach. A Model for Structure and Thermodynamics of ssDNA and dsDNA Near a Surface:A Coarse...

209

CMB Anisotropies from Outflows in Lyman Break Galaxies  

E-Print Network [OSTI]

Thomson scattering of the Cosmic Microwave Background (CMB) on moving electrons in the outflows of Lyman Break Galaxies (LBGs) at redshifts 2-8 contributes to the small-scale CMB anisotropies. The net effect produced by each outflow depends on its level of deviation from spherical symmetry, caused either by an anisotropic energy injection from the nuclear starburst or quasar activity, or by an inhomogeneous intergalactic environment. We find that for plausible outflow parameters consistent with spectroscopic observations of LBGs, the induced CMB anisotropies on arcminute scales reach up to $\\sim 1 \\mu$K, comparable to the level produced during the epoch of reionization.

Daniel Babich; Abraham Loeb

2006-09-14T23:59:59.000Z

210

CPT- and Lorentz-symmetry breaking: a review  

E-Print Network [OSTI]

The breakdown of spacetime symmetries has recently been identified as a promising candidate signal for underlying physics, possibly arising through quantum-gravitational effects. This talk gives an overview over various aspects of CPT- and Lorentz-violation research. Particular emphasis is given to the interplay between CPT, Lorentz, and translation symmetry, mechanisms for CPT and Lorentz breaking, and the construction of a low-energy quantum-field description of such effect. This quantum field framework, called the SME, is employed to determine possible phenomenological consequences of CPT and Lorentz violation for neutral-meson interferometry.

Ralf Lehnert

2006-12-28T23:59:59.000Z

211

Q-ball Instability due to U(1) Breaking  

E-Print Network [OSTI]

Q-ball is a non-topological soliton whose stability is ensured by global U(1) symmetry. We study a Q-ball which arises in the Affleck-Dine mechanism for baryogenesis and consider its possible instability due to U(1) breaking term ($A$-term) indispensable for successful baryogenesis. It is found that the instability destroys the Q-ball if its growth rate exceeds inverse of the typical relaxation time scale of the Q-ball. However, the instability is not so strong as it obstructs the cosmological formation of the Q-balls.

Masahiro Kawasaki; Kenichiro Konya; Fuminobu Takahashi

2005-05-24T23:59:59.000Z

212

Leak before break application in French PWR plants under operation  

SciTech Connect (OSTI)

Practical applications of the leak-before break concept are presently limited in French Pressurized Water Reactors (PWR) compared to Fast Breeder Reactors. Neithertheless, different fracture mechanic demonstrations have been done on different primary, auxiliary and secondary PWR piping systems based on similar requirements that the American NUREG 1061 specifications. The consequences of the success in different demonstrations are still in discussion to be included in the global safety assessment of the plants, such as the consequences on in-service inspections, leak detection systems, support optimization,.... A large research and development program, realized in different co-operative agreements, completes the general approach.

Faidy, C. [EDF SEPTEN, Villeurbanne (France)

1997-04-01T23:59:59.000Z

213

Spontaneous symmetry breaking and optimization of functional renormalization group  

E-Print Network [OSTI]

The requirement for the absence of spontaneous symmetry breaking in the d=1 dimension has been used to optimize the regulator dependence of functional renormalization group equations in the framework of the sine-Gordon scalar field theory. Results obtained by the optimization of this kind were compared to those of the Litim-Pawlowski and the principle of minimal sensitivity optimization scenarios. The optimal parameters of the compactly supported smooth (CSS) regulator, which recovers all major types of regulators in appropriate limits, have been determined beyond the local potential approximation, and the Litim limit of the CSS was found to be the optimal choice.

I. Nandori; I. G. Marian; V. Bacso

2014-02-27T23:59:59.000Z

214

Spontaneous symmetry breaking and collapse in bosonic Josephson junctions  

SciTech Connect (OSTI)

We investigate an attractive atomic Bose-Einstein condensate (BEC) trapped by a double-well potential in the axial direction and by a harmonic potential in the transverse directions. We obtain numerically a quantum phase diagram which includes all the three relevant phases of the system: Josephson, spontaneous symmetry breaking (SSB), and collapse. We consider also the coherent dynamics of the BEC and calculate the frequency of population-imbalance mode in the Josephson phase and in the SSB phase up to the collapse. We show that these phases can be observed by using ultracold vapors of {sup 7}Li atoms in a magneto-optical trap.

Mazzarella, Giovanni [Dipartimento di Fisica 'Galileo Galilei' and Consorzio Nazionale Interuniversitatio per le Scienze Fisiche della Materia (CNISM), Universita di Padova, Via Marzolo 8, I-35122 Padova (Italy); Salasnich, Luca [Dipartimento di Fisica ''Galileo Galilei'' and Consorzio Nazionale Interuniversitatio per le Scienze Fisiche della Materia (CNISM), Universita di Padova, Via Marzolo 8, I-35122 Padova (Italy); Istituto Nazionale di Ottica (INO) del Consiglio Nazionale delle Ricerche (CNR), Research Unit at Padova, Via Marzolo 8, I-35122 Padova (Italy); Center for Applied Mathematics and Theoretical Physics, University of Maribor, Krekova 2, SI-2000 Maribor (Slovenia)

2010-09-15T23:59:59.000Z

215

Charge symmetry breaking in n p --> d pi^0  

E-Print Network [OSTI]

The forward--backward asymmetry in n p --> d pi^0, which must be zero in the center-of-mass system if charge symmetry is respected, has been measured to be [17.2 +/- 8 (stat) +/- 5.5 (sys)] * 10^{-4}, at an incident neutron energy of 279.5 MeV. This charge symmetry breaking observable was extracted by fitting the data with GEANT-based simulations and is compared to recent chiral effective field theory calculations, with implications regarding the value of the u d quark mass difference.

A. K. Opper; E. Korkmaz; D. A. Hutcheon; R. Abegg; C. A. Davis; R. W. Finlay; P. W. Green; L. G. Greeniaus; D. V. Jordan; J. A. Niskanen; G. V. O'Rielly; T. A. Porcelli; S. D. Reitzner; P. L. Walden; S. Yen

2003-06-19T23:59:59.000Z

216

Ground Breaking of Blythe Solar Power Project | Department of Energy  

Office of Environmental Management (EM)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr May Jun Jul(Summary) "of Energy Power.pdf11-161-LNG | Department of EnergyGeothermalGoing OffGreen LeaseBreaking of Blythe

217

Breaking a Pocket of Resistance in the Fight Against Cancer  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr May JunDatastreamsmmcrcalgovInstrumentsruc DocumentationP-Series to someone6 M.ExtracellularBradbury Science MuseumBrainBreaking a Pocket

218

Breaking the ties that bind: New hope for biomass fuels  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr May JunDatastreamsmmcrcalgovInstrumentsruc DocumentationP-Series to someone6 M.ExtracellularBradbury Science MuseumBrainBreaking aNew hope

219

SB Electronics Breaks Ground on New Factory | Department of Energy  

Office of Environmental Management (EM)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr May Jun Jul(Summary) "of EnergyEnergyENERGY TAXBalancedDepartmentRestrictions onSB Electronics Breaks Ground on New Factory SB

220

Breaking the Biological Barriers to Cellulosic Ethanol, June 2006  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE:YearRound-Up fromDepartmentTie Ltd: ScopeDepartment1, DOE/SC-0095 Breakng the

Note: This page contains sample records for the topic "dna double-strand breaks" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


221

Symmetry Breaking of H2 Dissociation by a Single Photon  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr May JunDatastreamsmmcrcalgovInstrumentsrucLas ConchasPassiveSubmittedStatus TomAbout »LabSustainability Ames LaboratorySymmetry Breaking

222

Lack of Bystander Effects From High LET Radiation For Early Cytogenetic Endpoints.  

SciTech Connect (OSTI)

The aim of this work was to study radiation-induced bystander effects for early cytogenetic end points in various cell lines using the medium transfer technique after exposure to high- and low-LET radiation. Cells were exposed to 20 MeV/ nucleon nitrogen ions, 968 MeV/nucleon iron ions, or 575 MeV/nucleon iron ions followed by transfer of the conditioned medium from the irradiated cells to unirradiated test cells. The effects studied included DNA double-strand break induction, {gamma}-H2AX focus formation, induction of chromatid breaks in prematurely condensed chromosomes, and micronucleus formation using DNA repair-proficient and -deficient hamster and human cell lines (xrs6, V79, SW48, MO59K and MO59J). Cell survival was also measured in SW48 bystander cells using X rays. Although it was occasionally possible to detect an increase in chromatid break levels using nitrogen ions and to see a higher number of {gamma}-H2AX foci using nitrogen and iron ions in xrs6 bystander cells in single experiments, the results were not reproducible. After we pooled all the data, we could not verify a significant bystander effect for any of these end points. Also, we did not detect a significant bystander effect for DSB induction or micronucleus formation in these cell lines or for clonogenic survival in SW48 cells. The data suggest that DNA damage and cytogenetic changes are not induced in bystander cells. In contrast, data in the literature show pronounced bystander effects in a variety of cell lines, including clonogenic survival in SW48 cells and induction of chromatid breaks and micronuclei in hamster cells. To reconcile these conflicting data, it is possible that the epigenetic status of the specific cell line or the precise culture conditions and medium supplements, such as serum, may be critical for inducing bystander effects.

Groesser, Torsten; Cooper, Brian; Rydberg, Bjorn

2008-05-07T23:59:59.000Z

223

Integrated Microfluidic Electrochemical DNA Sensor  

E-Print Network [OSTI]

of small scale fluid flow Laminar flow Easy to predict the flow patterns Very little diffusion This can make mixing difficult Small volumes Don't need to waste expensive reagents Easy fluid control;DNA Purification The DNA will be extracted using Invitrogen Charge Switch beads. Cellular Lysis

Fygenson, Deborah Kuchnir

224

E-Print Network 3.0 - adducts strand breaks Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

its complement... to its high reactivity toward DNA and cellular proteins. The major acrolein-DNA adduct, -hydrox- ypropano... -hy- droxynonenal, and acrolein, that readily form...

225

Pulsar Emission above the Spectral Break - A Stacked Approach  

E-Print Network [OSTI]

NASA's Fermi space telescope has provided us with a bountiful new population of gamma-ray sources following its discovery of 150 new gamma-ray pulsars. One common feature exhibited by all of these pulsars is the form of their spectral energy distribution, which can be described by a power law followed by a spectral break occurring between $\\sim$1 and $\\sim$8 GeV. The common wisdom is that the break is followed by an exponential cut-off driven by radiation/reaction-limited curvature emission. The discovery of pulsed gamma rays from the Crab pulsar, the only pulsar so far detected at very high energies (E$>$100GeV), contradicts this "cutoff" picture. Here we present a new stacked analysis with an average of 4.2 years of data on 115 pulsars published in the 2nd LAT catalog of pulsars. This analysis is sensitive to low-level $\\sim$100 GeV emission which cannot be resolved in individual pulsars but can be detected from an ensemble.

McCann, Andrew

2015-01-01T23:59:59.000Z

226

Bending-induced Symmetry Breaking of Lithiation in Germanium Nanowires  

SciTech Connect (OSTI)

From signal transduction of living cells to oxidation and corrosion of metals, mechanical stress intimately couples with chemical reactions, regulating these biological and physiochemical processes. The coupled effect is particularly evident in electrochemical lithiation/delithiation cycling of high-capacity electrodes, such as silicon (Si), where on one hand lithiation-generated stress mediates lithiation kinetics, and on the other electrochemical reaction rate regulates stress generation and mechanical failure of the electrodes. Here we report for the first time the evidence on the controlled lithiation in germanium nanowires (GeNWs) through external bending. Contrary to the symmetric core-shell lithiation in free-standing GeNWs, we show bending GeNWs breaks the lithiation symmetry, speeding up lithaition at the tensile side while slowing down at the compressive side of the GeNWs. The bending-induced symmetry breaking of lithiation in GeNWs is further corroborated by chemomechanical modeling. In the light of the coupled effect between lithiation kinetics and mechanical stress in the electrochemical cycling, our findings shed light on strain/stress engineering of durable high-rate electrodes and energy harvesting through mechanical motion.

Gu, Meng; Yang, Hui; Perea, Daniel E.; Zhang, Jiguang; Zhang, Sulin; Wang, Chong M.

2014-08-01T23:59:59.000Z

227

Aerosol cluster impact and break-up : model and implementation.  

SciTech Connect (OSTI)

In this report a model for simulating aerosol cluster impact with rigid walls is presented. The model is based on JKR adhesion theory and is implemented as an enhancement to the granular (DEM) package within the LAMMPS code. The theory behind the model is outlined and preliminary results are shown. Modeling the interactions of small particles is relevant to a number of applications (e.g., soils, powders, colloidal suspensions, etc.). Modeling the behavior of aerosol particles during agglomeration and cluster dynamics upon impact with a wall is of particular interest. In this report we describe preliminary efforts to develop and implement physical models for aerosol particle interactions. Future work will consist of deploying these models to simulate aerosol cluster behavior upon impact with a rigid wall for the purpose of developing relationships for impact speed and probability of stick/bounce/break-up as well as to assess the distribution of cluster sizes if break-up occurs. These relationships will be developed consistent with the need for inputs into system-level codes. Section 2 gives background and details on the physical model as well as implementations issues. Section 3 presents some preliminary results which lead to discussion in Section 4 of future plans.

Lechman, Jeremy B.

2010-10-01T23:59:59.000Z

228

Enhancement of the RAD51 Recombinase Activity by the Tumor Suppressor PALB2  

SciTech Connect (OSTI)

Homologous recombination mediated by the RAD51 recombinase helps eliminate chromosomal lesions, such as DNA double-stranded breaks induced by radiation or arising from injured DNA replication forks. The tumor suppressors BRCA2 and PALB2 act together to deliver RAD51 to chromosomal lesions to initiate repair. Here we document a new function of PALB2 in the enhancement of RAD51's ability to form the D-loop. We show that PALB2 binds DNA and physically interacts with RAD51. Importantly, while PALB2 alone stimulates D-loop formation, a cooperative effect is seen with RAD51AP1, an enhancer of RAD51. This stimulation stems from PALB2's ability to function with RAD51 and RAD51AP1 to assemble the synaptic complex. Our results help unveil a multi-faceted role of PALB2 in chromosome damage repair. Since PALB2 mutations can cause breast and other tumors or lead to Fanconi anemia, our findings are important for understanding the mechanism of tumor suppression in humans.

Dray, Eloise; Etchin, Julia; Wiese, Claudia; Saro, Dorina; Williams, Gareth J.; Hammel, Michal; Yu, Xiong; Galkin, Vitold E.; Liu, Dongqing; Tsai, Miaw-Sheue; Sy, Shirley M-H.; Egelman, Edward; Chen, Junjie; Sung, Patrick; Schild, D.

2010-08-24T23:59:59.000Z

229

CSNI specialist meeting on leak-before-break in nuclear reactor piping: proceedings  

SciTech Connect (OSTI)

On September 1 and 2, 1983, the CSNI subcommittee on primary system integrity held a special meeting in Monterey, California, on the subject of leak-before-break in nuclear reactor piping systems. The purpose of the meeting was to provide an international forum for the exchange of ideas, positions, and research results; to identify areas requiring additional research and development; and to determine the general attitude toward acceptance of the leak-before-break concept. The importance of the leak-before-break issue was evidenced by excellent attendance at the meeting and through active participation by the meeting attendees. Approximately 125 people representing fifteen different nations attended the meeting. The meeting was divided into four technical sessions addressing the following areas: Application of Piping Fracture Mechanics to Leak-Before Break, Leak Rate and Leak Detection, Leak-Before-Break Studies, Methods and Results, Current and Proposed Positions on Leak-Before-Break.

Not Available

1984-08-01T23:59:59.000Z

230

Analytical Derivation of Three Dimensional Vorticity Function for wave breaking in Surf Zone  

E-Print Network [OSTI]

In this report, Mathematical model for generalized nonlinear three dimensional wave breaking equations was de- veloped analytically using fully nonlinear extended Boussinesq equations to encompass rotational dynamics in wave breaking zone. The three dimensional equations for vorticity distributions are developed from Reynold based stress equations. Vorticity transport equations are also developed for wave breaking zone. This equations are basic model tools for numerical simulation of surf zone to explain wave breaking phenomena. The model reproduces most of the dynamics in the surf zone. Non linearity for wave height predictions is also shown close to the breaking both in shoaling as well as surf zone. Keyword Wave breaking, Boussinesq equation, shallow water, surf zone. PACS : 47.32-y

Dutta, R

2015-01-01T23:59:59.000Z

231

ON THE EVOLUTION OF THE SPECTRAL BREAK IN THE AFTERGLOW OF GAMMA-RAY BURSTS  

SciTech Connect (OSTI)

The temporal evolution of the spectral break in the time-resolved spectral energy density of the broadband afterglow of gamma-ray bursts (GRBs) 091127 and 080319B was shown recently to be inconsistent with that expected for the cooling break in the standard fireball model of GRBs. Here we show that it is, however, in good agreement with the predicted temporal evolution of the smooth injection break/bend in the cannonball model of GRBs.

Dado, Shlomo; Dar, Arnon [Physics Department, Technion, Haifa 32000 (Israel)

2012-02-20T23:59:59.000Z

232

Planetary Wave Breaking and Nonlinear Reflection: Seasonal Cycle and Interannual Variability  

E-Print Network [OSTI]

and L. M. Polvani, 2000: Climatology of intrusions into theM. H. Hitchman, 1999: A climatology of Rossby wave breaking

Abatzoglou, John T; Magnusdottir, Gudrun

2006-01-01T23:59:59.000Z

233

Bubbles Help Break Energy Storage Record for Lithium Air-Batteries  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Bubbles Help Break Energy Storage Record for Lithium Air-Batteries Foam-base graphene keeps oxygen flowing in batteries that holds promise for electric vehicles January...

234

DNA UPTAKE BY TRANSFORMABLE BACTERIA  

SciTech Connect (OSTI)

The various processes of DNA uptake by cells can be categorized as: viral DNA entry, conjugation, or transformation. Within each category, a variety of mechanisms have been found. However, considerable similarities occur among the different mechanisms of conjugation and, especially, transformation. All of these natural mechanisms of DNA transfer are quite elaborate and involve multiple protein components, as the case may be, of the virus, the donor cell, and the recipient cell. The mechanisms of viral infection and conjugation will be discussed mainly with respect to their relevance to transformation.

LACKS,S.A.

1999-09-07T23:59:59.000Z

235

Sequence independent amplification of DNA  

DOE Patents [OSTI]

The present invention is a rapid sequence-independent amplification procedure (SIA). Even minute amounts of DNA from various sources can be amplified independent of any sequence requirements of the DNA or any a priori knowledge of any sequence characteristics of the DNA to be amplified. This method allows, for example, the sequence independent amplification of microdissected chromosomal material and the reliable construction of high quality fluorescent in situ hybridization (FISH) probes from YACs or from other sources. These probes can be used to localize YACs on metaphase chromosomes but also--with high efficiency--in interphase nuclei. 25 figs.

Bohlander, S.K.

1998-03-24T23:59:59.000Z

236

Sequence independent amplification of DNA  

DOE Patents [OSTI]

The present invention is a rapid sequence-independent amplification procedure (SIA). Even minute amounts of DNA from various sources can be amplified independent of any sequence requirements of the DNA or any a priori knowledge of any sequence characteristics of the DNA to be amplified. This method allows, for example the sequence independent amplification of microdissected chromosomal material and the reliable construction of high quality fluorescent in situ hybridization (FISH) probes from YACs or from other sources. These probes can be used to localize YACs on metaphase chromosomes but also--with high efficiency--in interphase nuclei.

Bohlander, Stefan K. (Chicago, IL)

1998-01-01T23:59:59.000Z

237

Normalized cDNA libraries  

DOE Patents [OSTI]

This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3' noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library.

Soares, Marcelo B. (New York, NY); Efstratiadis, Argiris (Englewood, NJ)

1997-01-01T23:59:59.000Z

238

Normalized cDNA libraries  

DOE Patents [OSTI]

This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3{prime} noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library. 4 figs.

Soares, M.B.; Efstratiadis, A.

1997-06-10T23:59:59.000Z

239

A Symmetry Breaking Model for X Chromosome Inactivation  

E-Print Network [OSTI]

In mammals, dosage compensation of X linked genes in female cells is achieved by inactivation of one of their two X chromosomes which is randomly chosen. The earliest steps in X-inactivation (XCI), namely the mechanism whereby cells count their X chromosomes and choose between two equivalent X, remain mysterious. Starting from the recent discovery of X chromosome colocalization at the onset of X-inactivation, we propose a Statistical Mechanics model of XCI, which is investigated by computer simulations and checked against experimental data. Our model describes how a `blocking factor' complex is self-assembled and why only one is formed out of many diffusible molecules, resulting in a spontaneous symmetry breaking (SB) in the binding to two identical chromosomes. These results are used to derive a scenario of biological implications describing all current experimental evidences, e.g., the importance of colocalization.

M. Nicodemi; A. Prisco

2008-01-17T23:59:59.000Z

240

Radiative PQ Breaking and the Higgs Boson Mass  

E-Print Network [OSTI]

The small and negative value of the Standard Model Higgs quartic coupling at high scales can be understood in terms of anthropic selection on a landscape where large and negative values are favored: most universes have a very short-lived electroweak vacuum and typical observers are in universes close to the corresponding metastability boundary. We provide a simple example of such a landscape with a Peccei-Quinn symmetry breaking scale generated through dimensional transmutation and supersymmetry softly broken at an intermediate scale. Large and negative contributions to the Higgs quartic are typically generated on integrating out the saxion field. Cancellations among these contributions are forced by the anthropic requirement of a sufficiently long-lived electroweak vacuum, determining the multiverse distribution for the Higgs quartic in a similar way to that of the cosmological constant. This leads to a statistical prediction of the Higgs boson mass that, for a wide range of parameters, yields the observed v...

D'Eramo, Francesco; Pappadopulo, Duccio

2015-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "dna double-strand breaks" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


241

Inertial blob-hole symmetry breaking in magnetised plasma filaments  

E-Print Network [OSTI]

Symmetry breaking between the propagation velocities of magnetised plasma filaments with large positive (blob) and negative (hole) amplitudes, as implied by a dimensional analysis scaling, is studied with global ("full-n") non-Boussinesq gyrofluid computations, which include finite inertia effects through nonlinear polarisation. Interchange blobs on a flat density background have higher inertia and propagate more slowly than holes. In the presence of a large enough density gradient, the effect is reversed: blobs accelerate down the gradient and holes are slowed in their propagation up the gradient. Drift wave blobs spread their initial vorticity rapidly into a fully developed turbulent state, whereas primary holes can remain coherent for many eddy turnover times. The results bear implications for plasma edge zonal flow evolution and tokamak scrape-off-layer transport.

Kendl, Alexander

2015-01-01T23:59:59.000Z

242

Modeling DNA Shuffling Fengzhu Sun  

E-Print Network [OSTI]

Modeling DNA Shuffling Fengzhu Sun 1Department of Genetics Emory University School of Medicine property are selected. Irvine et al. (1991) and Sun et al. (1996) studied in vitro evolution not involving

Sun, Fengzhu - Sun, Fengzhu

243

Unnatural nucleotides for DNA sequencing  

E-Print Network [OSTI]

Fluorescent nucleotide analogs were prepared and tested to find surrogate structures that are: (i) incorporated by DNA polymerases; (ii) spectroscopically distinct for each fluorescent tag; and (iii) easily deprotected at the 3'-position under mild...

Jacutin, Swanee E

1997-01-01T23:59:59.000Z

244

Molecular Computing with DNA Self-Assembly  

E-Print Network [OSTI]

Molecular Computing with DNA Self-Assembly Urmi Majumder #12;Self-Assembly in Nature #12;Key to DNA for Molecular Computing with DNA Self-Assembly Compact: Small library of assembly primitives Complex: Capable in Tiling Assembly: vitroation tural DNA self-assembly has powerful echanisms for error correction

Reif, John H.

245

CONSULTATION RESPONSE The Forensic Use of DNA and the National DNA Database  

E-Print Network [OSTI]

CONSULTATION RESPONSE The Forensic Use of DNA and the National DNA Database Wellcome Trust response on the important topic of the forensic use of DNA and the National DNA Database (NDNAD). Given the Trust Assembly; "Forensic DNA Databasing: A European perspective" - a biomedical ethics grant to Professor Robin

Rambaut, Andrew

246

Design Considerations for Monopile Founded Offshore Wind Turbines Subject to Breaking Waves  

E-Print Network [OSTI]

DESIGN CONSIDERATIONS FOR MONOPILE FOUNDED OFFSHORE WIND TURBINES SUBJECT TO BREAKING WAVES A Thesis by GARRETT REESE OWENS Submitted to the Office of Graduate Studies of Texas A&M University in partial fulfillment... Farms ...... 4 Figure 2 Overview of Offshore Wind Turbine Terminology................................. 7 Figure 3 Overturning Moment as a Function of Water Depth ............................... 10 Figure 4 Types of Breaking Waves...

Owens, Garrett Reese 1987-

2012-11-26T23:59:59.000Z

247

Symmetry Breaking in Graphene Gregory S. Boebinger, National High Magnetic Field Laboratory  

E-Print Network [OSTI]

Symmetry Breaking in Graphene Gregory S. Boebinger, National High Magnetic Field Laboratory DMR. In this experiment, Landau levels in graphene were used to study the breaking of SU(4) symmetry--a higher dimensional states by their spin polarization. It was found that graphene turns into either a spin ferromagnet

Weston, Ken

248

Break-Even Cost for Residential Photovoltaics in the United States: Key Drivers and Sensitivities  

SciTech Connect (OSTI)

Grid parity--or break-even cost--for photovoltaic (PV) technology is defined as the point where the cost of PV-generated electricity equals the cost of electricity purchased from the grid. Break-even cost is expressed in $/W of an installed system. Achieving break-even cost is a function of many variables. Consequently, break-even costs vary by location and time for a country, such as the United States, with a diverse set of resources, electricity prices, and other variables. In this report, we analyze PV break-even costs for U.S. residential customers. We evaluate some key drivers of grid parity both regionally and over time. We also examine the impact of moving from flat to time-of-use (TOU) rates, and we evaluate individual components of the break-even cost, including effect of rate structure and various incentives. Finally, we examine how PV markets might evolve on a regional basis considering the sensitivity of the break-even cost to four major drivers: technical performance, financing parameters, electricity prices and rates, and policies. We find that local incentives rather than ?technical? parameters are in general the key drivers of the break-even cost of PV. Additionally, this analysis provides insight about the potential viability of PV markets.

Denholm, P.; Margolis, R. M.; Ong, S.; Roberts, B.

2009-12-01T23:59:59.000Z

249

Generation, propagation, and breaking of an internal wave beam Heather A. Clark and Bruce R. Sutherlanda  

E-Print Network [OSTI]

Generation, propagation, and breaking of an internal wave beam Heather A. Clark and Bruce R fluids support the propagation of inter- nal gravity waves that arise from buoyancy restoring forces non- local effect on the mean flow through the propagation and breaking of internal waves. While

Sutherland, Bruce

250

Hydrogen bond breaking probed with multidimensional stimulated vibrational echo correlation spectroscopy  

E-Print Network [OSTI]

Hydrogen bond breaking probed with multidimensional stimulated vibrational echo correlation September 2003 Hydrogen bond population dynamics are extricated with exceptional detail using ultrafast ( 50 of methanol­OD oligomers in CCl4 . Hydrogen bond breaking makes it possible to acquire data for times much

Fayer, Michael D.

251

Formation of beads-on-a-string structures during break-up of viscoelastic filaments  

E-Print Network [OSTI]

Break-up of viscoelastic filaments is pervasive in both nature and technology. If a filament is formed by placing a drop of saliva between a thumb and forefinger and is stretched, the filament’s morphology close to break-up ...

Bhat, Pardeep P.

252

DNA polymorphism identity determination using flow cytometry  

DOE Patents [OSTI]

DNA polymorphism identity determination using flow cytometry. Primers designed to be immobilized on microspheres are allowed to anneal to the DNA strand under investigation, and are extended by either DNA polymerase using fluorescent dideoxynucleotides or ligated by DNA ligase to fluorescent reporter oligonucleotides. The fluorescence of either the dideoxynucleotide or the reporter oligonucleotide attached to the immobilized primer is measured by flow cytometry, thereby identifying the nucleotide polymorphism on the DNA strand.

Nolan, John P. (Santa Fe, NM); White, P. Scott (Los Alamos, NM); Cai, Hong (Los Alamos, NM)

2001-01-01T23:59:59.000Z

253

Cellular responses to environmental DNA damage  

SciTech Connect (OSTI)

This volume contains the proceedings of the conference entitled Cellular Responses to Environmental DNA Damage held in Banff,Alberta December 1--6, 1991. The conference addresses various aspects of DNA repair in sessions titled DNA repair; Basic Mechanisms; Lesions; Systems; Inducible Responses; Mutagenesis; Human Population Response Heterogeneity; Intragenomic DNA Repair Heterogeneity; DNA Repair Gene Cloning; Aging; Human Genetic Disease; and Carcinogenesis. Individual papers are represented as abstracts of about one page in length.

Not Available

1994-08-01T23:59:59.000Z

254

Mobilization of Viable Tumor Cells Into the Circulation During Radiation Therapy  

SciTech Connect (OSTI)

Purpose: To determine whether radiation therapy (RT) could mobilize viable tumor cells into the circulation of non-small cell lung cancer (NSCLC) patients. Methods and Materials: We enumerated circulating tumor cells (CTCs) by fluorescence microscopy of blood samples immunostained with conventional CTC markers. We measured their DNA damage levels using ?-H2AX, a biomarker for radiation-induced DNA double-strand breaks, either by fluorescence-activated cell sorting or by immunofluorescence microscopy. Results: Twenty-seven RT-treated NSCLC patients had blood samples analyzed by 1 or more methods. We identified increased CTC numbers after commencement of RT in 7 of 9 patients treated with palliative RT, and in 4 of 8 patients treated with curative-intent RT. Circulating tumor cells were also identified, singly and in clumps in large numbers, during RT by cytopathologic examination (in all 5 cases studied). Elevated ?-H2AX signal in post-RT blood samples signified the presence of CTCs derived from irradiated tumors. Blood taken after the commencement of RT contained tumor cells that proliferated extensively in vitro (in all 6 cases studied). Circulating tumor cells formed ?-H2AX foci in response to ex vivo irradiation, providing further evidence of their viability. Conclusions: Our findings provide a rationale for the development of strategies to reduce the concentration of viable CTCs by modulating RT fractionation or by coadministering systemic therapies.

Martin, Olga A. [Division of Radiation Oncology and Cancer Imaging, Peter MacCallum Cancer Centre, East Melbourne, VIC (Australia); Molecular Radiation Biology Laboratory, Peter MacCallum Cancer Centre, East Melbourne, VIC (Australia); The Sir Peter MacCallum Department of Oncology, University of Melbourne, Melbourne, VIC (Australia); Anderson, Robin L. [The Sir Peter MacCallum Department of Oncology, University of Melbourne, Melbourne, VIC (Australia); Metastasis Research Laboratory, Peter MacCallum Cancer Centre, East Melbourne, VIC (Australia); Russell, Prudence A. [Department of Anatomical Pathology, St. Vincent Hospital, Fitzroy, VIC (Australia); Ashley Cox, R. [Division of Radiation Oncology and Cancer Imaging, Peter MacCallum Cancer Centre, East Melbourne, VIC (Australia); Ivashkevich, Alesia [Molecular Radiation Biology Laboratory, Peter MacCallum Cancer Centre, East Melbourne, VIC (Australia); Laboratory of DNA Repair and Genomics, Centre for Innate Immunity and Infectious Disease, Monash Institute for Medical Research, Monash University, Clayton, VIC (Australia); Swierczak, Agnieszka; Doherty, Judy P. [Metastasis Research Laboratory, Peter MacCallum Cancer Centre, East Melbourne, VIC (Australia); Jacobs, Daphne H.M. [Division of Radiation Oncology and Cancer Imaging, Peter MacCallum Cancer Centre, East Melbourne, VIC (Australia); Smith, Jai [Molecular Radiation Biology Laboratory, Peter MacCallum Cancer Centre, East Melbourne, VIC (Australia); Siva, Shankar; Daly, Patricia E. [Division of Radiation Oncology and Cancer Imaging, Peter MacCallum Cancer Centre, East Melbourne, VIC (Australia); Ball, David L. [Division of Radiation Oncology and Cancer Imaging, Peter MacCallum Cancer Centre, East Melbourne, VIC (Australia); The Sir Peter MacCallum Department of Oncology, University of Melbourne, Melbourne, VIC (Australia); and others

2014-02-01T23:59:59.000Z

255

Comparison of protein patterns of xrs-5, a radiosensitive Chinese hamster ovary cell line, and CHO-K1, its radioresistant parent, using two-dimensional gel-electrophoresis  

SciTech Connect (OSTI)

X-ray sensitive strains of Chinese hamster ovary cell lines have been used to analyze radiation repair mechanisms. One cell line, xrs-5, has been shown to be very sensitive to ionizing radiation and radical forming chemical mutagens. This sensitivity is thought to be a result a mutation in the DNA double strand break (DSB) repair mechanism, and its characterization has been a goal of several repair mechanism studies. Using two-dimensional gel electrophoresis, we have detected a protein (MW approximately 55KD) in the DNA/Nuclear Matrix (nucleoid) cell fraction of CHO-Kl cells that is absent in the nucleoid fraction of xrs-5. This protein is present, however, in both CHO-Kl and xrs-5 whole cell protein maps. To determine whether the 55KD protein is responsible for the radiosensitive and defective DSB repair phenotype of xrs-5 cells, studies are now underway to analyze revertants of xrs-5 that are proficient in DSB repair. Furthermore, an effort to sequence the protein in question is planned. 23 refs., 2 figs.

Kramer, J.M. (Miami Univ., Oxford, OH (USA). Dept. of Zoology)

1991-01-01T23:59:59.000Z

256

Co-targeting Deoxyribonucleic Acid–Dependent Protein Kinase and Poly(Adenosine Diphosphate-Ribose) Polymerase-1 Promotes Accelerated Senescence of Irradiated Cancer Cells  

SciTech Connect (OSTI)

Purpose: To examine the effects of combined blockade of DNA-dependent protein kinase (DNA-PK) and poly(adenosine diphosphate-ribose) polymerase-1 (PARP-1) on accelerated senescence in irradiated H460 and A549 non-small cell lung cancer cells. Methods and Materials: The effects of KU5788 and AG014699 (inhibitors of DNA-PK and PARP-1, respectively) on clonogenic survival, DNA double-strand breaks (DSBs), apoptosis, mitotic catastrophe, and accelerated senescence in irradiated cells were examined in vitro. For in vivo experiments, H460 xenografts established in athymic nude mice were treated with BEZ235 (a DNA-PK, ATM, and phosphatidylinositol 3-kinase/mammalian target of rapamycin inhibitor) and AG014699 to determine effects on proliferation, DNA DSBs, and accelerated senescence after radiation. Results: Compared with either inhibitor alone, combination treatment with KU57788 and AG014699 reduced postradiation clonogenic survival and significantly increased persistence of Gamma-H2AX (?H2AX) foci in irradiated H460 and A549 cells. Notably, these effects coincided with the induction of accelerated senescence in irradiated cells as reflected by positive ?-galactosidase staining, G2-M cell-cycle arrest, enlarged and flattened cellular morphology, increased p21 expression, and senescence-associated cytokine secretion. In irradiated H460 xenografts, concurrent therapy with BEZ235 and AG014699 resulted in sustained Gamma-H2AX (?H2AX) staining and prominent ?-galactosidase activity. Conclusion: Combined DNA-PK and PARP-1 blockade increased tumor cell radiosensitivity and enhanced the prosenescent properties of ionizing radiation in vitro and in vivo. These data provide a rationale for further preclinical and clinical testing of this therapeutic combination.

Azad, Arun, E-mail: arun.azad@bccancer.bc.ca [Division of Cancer Research, Peter MacCallum Cancer Centre, East Melbourne, Victoria (Australia); Department of Pathology, St. Vincent's Hospital, University of Melbourne, Parkville, Victoria (Australia); Bukczynska, Patricia; Jackson, Susan [Division of Cancer Research, Peter MacCallum Cancer Centre, East Melbourne, Victoria (Australia); Haput, Ygal; Cullinane, Carleen [Division of Cancer Research, Peter MacCallum Cancer Centre, East Melbourne, Victoria (Australia); Sir Peter MacCallum Department of Oncology, University of Melbourne, Parkville, Victoria (Australia); McArthur, Grant A.; Solomon, Benjamin [Division of Cancer Research, Peter MacCallum Cancer Centre, East Melbourne, Victoria (Australia); Division of Cancer Medicine, Peter MacCallum Cancer Centre, East Melbourne, Victoria (Australia); Department of Medicine, St. Vincent's Hospital, University of Melbourne, Parkville, Victoria (Australia); Sir Peter MacCallum Department of Oncology, University of Melbourne, Parkville, Victoria (Australia)

2014-02-01T23:59:59.000Z

257

Regulation of DNA damage tolerance : studies of the translesion synthesis DNA ploymerase eta in Saccharomyces cerevisiae  

E-Print Network [OSTI]

All organisms must control the effects of DNA damage to protect the integrity of their genomes. In addition to DNA repair, this requires DNA damage tolerance pathways, which allow the continuation of essential processes ...

Woodruff, Rachel Van Etten

2008-01-01T23:59:59.000Z

258

Physical Naturalness and Dynamical Breaking of Classical Scale Invariance  

E-Print Network [OSTI]

We propose a model of a confining dark sector, dark technicolor, that communicates with the Standard Model through the Higgs portal. In this model electroweak symmetry breaking and dark matter share a common origin, and the electroweak scale is generated dynamically. Our motivation to suggest this model is the absense of evidence for new physics from recent LHC data. Although the conclusion is far from certain at this point, this lack of evidence may suggest that no mechanism exists at the electroweak scale to stabilise the Higgs mass against radiative corrections from UV physics. The usual reaction to this puzzling situation is to conclude that the stabilising new physics is either hidden from us by accident, or that it appears at energies that are currently inaccessible, such that nature is indeed fine-tuned. In order to re-examine the arguments that have lead to this dichotomy, we review the concept of naturalness in effective field theories, discussing in particular the role of quadratic divergences in relation to different energy scales. This leads us to suggest classical scale invariance as a guidline for model building, implying that explicit mass scales are absent in the underlying theory.

Matti Heikinheimo; Antonio Racioppi; Martti Raidal; Christian Spethmann; Kimmo Tuominen

2014-05-05T23:59:59.000Z

259

Natural cold baryogenesis from strongly interacting electroweak symmetry breaking  

SciTech Connect (OSTI)

The mechanism of ''cold electroweak baryogenesis'' has been so far unpopular because its proposal has relied on the ad-hoc assumption of a period of hybrid inflation at the electroweak scale with the Higgs acting as the waterfall field. We argue here that cold baryogenesis can be naturally realized without the need to introduce any slow-roll potential. Our point is that composite Higgs models where electroweak symmetry breaking arises via a strongly first-order phase transition provide a well-motivated framework for cold baryogenesis. In this case, reheating proceeds by bubble collisions and we argue that this can induce changes in Chern-Simons number, which in the presence of new sources of CP violation commonly lead to baryogenesis. We illustrate this mechanism using as a source of CP violation an effective dimension-six operator which is free from EDM constraints, another advantage of cold baryogenesis compared to the standard theory of electroweak baryogenesis. Our results are general as they do not rely on any particular UV completion but only on a stage of supercooling ended by a first-order phase transition in the evolution of the universe, which can be natural if there is nearly conformal dynamics at the TeV scale. Besides, baryon-number violation originates from the Standard Model only.

Konstandin, Thomas; Servant, Géraldine, E-mail: tkonstan@cern.ch, E-mail: geraldine.servant@cern.ch [CERN Physics Department, Theory Division, CH-1211 Geneva 23 (Switzerland)

2011-07-01T23:59:59.000Z

260

Sea quark transverse momentum distributions and dynamical chiral symmetry breaking  

SciTech Connect (OSTI)

Recent theoretical studies have provided new insight into the intrinsic transverse momentum distributions of valence and sea quarks in the nucleon at a low scale. The valence quark transverse momentum distributions (q - qbar) are governed by the nucleon's inverse hadronic size R{sup -1} ~ 0.2 GeV and drop steeply at large p{sub T}. The sea quark distributions (qbar) are in large part generated by non-perturbative chiral-symmetry breaking interactions and extend up to the scale rho{sup -1} ~ 0.6 GeV. These findings have many implications for modeling the initial conditions of perturbative QCD evolution of TMD distributions (starting scale, shape of p{sub T}. distributions, coordinate-space correlation functions). The qualitative difference between valence and sea quark intrinsic p{sub T}. distributions could be observed experimentally, by comparing the transverse momentum distributions of selected hadrons in semi-inclusive deep-inelastic scattering, or those of dileptons produced in pp and pbar-p scattering.

Schweitzer, Peter [Univ. of Connecticut, Storrs, CT (United States); Strikman, Mark [Penn State Univ., State College, PA (United States); Weiss, Christian [JLAB Newport News, VA (United States)

2014-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "dna double-strand breaks" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


261

Breaking FIR-Radio Correlation: The Case of Interacting Galaxies  

E-Print Network [OSTI]

Far-infrared (FIR)--radio correlation is a well-established empirical connection between continuum radio and dust emission of star-forming galaxies, used as a tool in determining star-formation rates. Here we point out that in the case of interacting star-forming galaxies this tool might break. Galactic interactions and mergers have been known to give rise to tidal shocks and disrupt morphologies especially in the smaller of the interacting components. Moreover, these shocks can also heat the gas and dust and accelerate particles leading to tidal cosmic-ray population in addition to standard galactic cosmic rays. Both heating and additional non-thermal radiation will obviously affect the FIR-radio correlation of these systems. To test this hypothesis we have analyzed a sample of 43 infrared bright star-forming interacting galaxies at different merger stages. We have found that their FIR-radio correlation parameter and radio emission spectral index vary over different merger stages and behave as it would be ex...

Donevski, Darko

2015-01-01T23:59:59.000Z

262

Automated inspection of surface breaking cracks using GMR sensor arrays  

SciTech Connect (OSTI)

We present a prototype for automated magnetic stray field testing of ferromagnetic roller bearings. For this purpose NDE-adapted GMR sensor arrays (giant magneto resistance) are used for the detection of surface breaking cracks. The sensors are miniaturized down to the lower ?m-regime to achieve adequate spatial resolution. In doing so, sensor arrays with up to 48 elements are used to inspect the bearing surface within a few seconds only. In contrast to magnetic particle inspection (MPI), where the global magnetization requires a further inspection step and succeeding demagnetization, the presented prototype only locally magnetize the surface area in the vicinity of the GMR Sensors. For the local magnetization, the applied sub-surface magnetic field was simulated and proofed for detecting flaws with a depth of a few 10 ?m. By multiplexing the sensor array with an adapted read out electronics we quasi simultaneously detect the normal field component of about 100?m above the surface. The detection of artificial notches with a depth of 40 ?m and more could be resolved with a SNR better than 20 dB. The presented testing facility is fast and provides a step towards automated testing of safety relevant steel components.

Pelkner, Matthias; Reimund, Verena; Erthner, Thomas; Panke, Nicolai; Kreutzbruck, Marc [BAM Federal Institute for Material Research and Testing, Unter den Eichen 87, 12205 Berlin (Germany)

2014-02-18T23:59:59.000Z

263

Scales of Fermion Mass Generation and Electroweak Symmetry Breaking  

E-Print Network [OSTI]

The scale of mass generation for fermions (including neutrinos) and the scale for electroweak symmetry breaking (EWSB) can be bounded from above by the unitarity of scattering involving longitudinal weak gauge bosons or their corresponding would-be Goldstone bosons. Including the exact n-body phase space we analyze the 2 --> n ($n \\geq 2$) processes for the fermion-(anti)fermion scattering into multiple gauge boson final states. Contrary to naive energy power counting, we demonstrate that as $n$ becomes large, the competition between an increasing energy factor and a phase-space suppression leads to a {\\it strong new upper bound} on the scale of fermion mass generation at a finite value $n=n_s$, which is {\\it independent of the EWSB scale,} $v = (\\sqrt{2}G_F)^{-1/2}$. For quarks, leptons and Majorana neutrinos, the strongest 2 --> n limits range from about 3TeV to 130-170TeV (with $2\\lesssim n_s \\lesssim 24$), depending on the measured fermion masses. Strikingly, given the tiny neutrino masses as constrained by the neutrino oscillations, neutrinoless double-beta decays and astrophysical observations, the unitarity violation of $\

Duane A. Dicus; Hong-Jian He

2005-06-11T23:59:59.000Z

264

Treatment methods for breaking certain oil and water emulsions  

DOE Patents [OSTI]

Disclosed are treatment methods for breaking emulsions of petroleum oil and salt water, fatty oil and water, and those resulting from liquefication of organic material. The emulsions are broken by heating to a predetermined temperature at or above about 200.degree. C. and pressurizing to a predetermined pressure above the vapor pressure of water at the predetermined temperature to produce a heated and pressurized fluid. The heated and pressurized fluid is contained in a single vessel at the predetermined temperature and pressure for a predetermined period of time to effectively separate the emulsion into substantially distinct first and second phases, the first phase comprising primarily the petroleum oil, the second phase comprising primarily the water. The first and second phases are separately withdrawn from the vessel at a withdraw temperature between about 200.degree. C. and 374.degree. C. and a withdraw pressure above the vapor pressure of water at the withdraw temperature. Where solids are present in the certain emulsions, the above described treatment may also effectively separate the certain emulsion into a substantially distinct third phase comprising primarily the solids.

Sealock, Jr., L. John (W. Richland, WA); Baker, Eddie G. (Richland, WA); Elliott, Douglas C. (Richland, WA)

1992-01-01T23:59:59.000Z

265

Spontaneous symmetry breaking in Schroedinger lattices with two nonlinear sites  

SciTech Connect (OSTI)

We introduce discrete systems in the form of straight (infinite) and ring-shaped chains, with two symmetrically placed nonlinear sites. The systems can be implemented in nonlinear optics (as waveguiding arrays) and Bose-Einstein condensates (by means of an optical lattice). A full set of exact analytical solutions for symmetric, asymmetric, and antisymmetric localized modes is found, and their stability is investigated in a numerical form. The symmetry-breaking bifurcation, through which the asymmetric modes emerge from the symmetric ones, is found to be of the subcritical type. It is transformed into a supercritical bifurcation if the nonlinearity is localized in relatively broad domains around two central sites, and also in the ring of a small size, i.e., in effectively nonlocal settings. The family of antisymmetric modes does not undergo bifurcations and features both stable and unstable portions. The evolution of unstable localized modes is investigated by means of direct simulations. In particular, unstable asymmetric states, which exist in the case of the subcritical bifurcation, give rise to breathers oscillating between the nonlinear sites, thus restoring an effective dynamical symmetry between them.

Brazhnyi, Valeriy A.; Malomed, Boris A. [Centro de Fisica do Porto, Faculdade de Ciencias, Universidade do Porto, R. Campo Alegre 687, Porto 4169-007 (Portugal); ICFO-Institut de Ciencies Fotoniques, Universitat Politecnica de Catalunya, Mediterranean Technology Park, 08860 Castelldefels (Barcelona) (Spain) and Department of Physical Electronics, School of Electrical Engineering, Faculty of Engineering, Tel Aviv University, Tel Aviv 69978 (Israel)

2011-05-15T23:59:59.000Z

266

ADVANCES IN ATMOSPHERIC SCIENCES, VOL. 26, NO. 4, 2009, 701706 Variability of Northeast China River Break-up Date  

E-Print Network [OSTI]

River Break-up Date WANG Huijun1,2 ( ¡) and SUN Jianqi1,2 (ê ) 1 Institute of Atmospheric Physics investigates the variability of the break-up dates of the rivers in Northeast China from their icebound states for the period of 1957­2005 and explores some potential explanatory mechanisms. Results show that the break-up

267

DNA Concentration By UV Spectrophotometry Measure Absorption  

E-Print Network [OSTI]

DNA Concentration By UV Spectrophotometry Measure Absorption: 1. Dilute DNA to 0.5 to 50 µg 2. Measure absorption at 260 nm (A260). Start by zeroing instrument with TE buffer or dH2O alone

Aris, John P.

268

Micropatterned cell arrays for detecting DNA damage  

E-Print Network [OSTI]

Numerous agents are capable of interacting with DNA and damaging it. Permanent changes in the DNA structure can be both mutagenic and cytotoxic; therefore, methods to measure the susceptibility of cells to mutations are ...

Mittal, Sukant

2008-01-01T23:59:59.000Z

269

Towards Privacy Preserving of Forensic DNA Databases  

E-Print Network [OSTI]

Protecting privacy of individuals is critical for forensic genetics. In a kinship/identity testing, related DNA profiles between user's query and the DNA database need to be extracted. However, unrelated profiles cannot be revealed to each other...

Liu, Sanmin

2012-02-14T23:59:59.000Z

270

Chromosome specific repetitive DNA sequences  

DOE Patents [OSTI]

A method is provided for determining specific nucleotide sequences useful in forming a probe which can identify specific chromosomes, preferably through in situ hybridization within the cell itself. In one embodiment, chromosome preferential nucleotide sequences are first determined from a library of recombinant DNA clones having families of repetitive sequences. Library clones are identified with a low homology with a sequence of repetitive DNA families to which the first clones respectively belong and variant sequences are then identified by selecting clones having a pattern of hybridization with genomic DNA dissimilar to the hybridization pattern shown by the respective families. In another embodiment, variant sequences are selected from a sequence of a known repetitive DNA family. The selected variant sequence is classified as chromosome specific, chromosome preferential, or chromosome nonspecific. Sequences which are classified as chromosome preferential are further sequenced and regions are identified having a low homology with other regions of the chromosome preferential sequence or with known sequences of other family me This invention is the result of a contract with the Department of Energy (Contract No. W-7405-ENG-36).

Moyzis, Robert K. (Los Alamos, NM); Meyne, Julianne (Los Alamos, NM)

1991-01-01T23:59:59.000Z

271

International Standards in Forensic DNA  

E-Print Network [OSTI]

International Standards in Forensic DNA John M. Butler, Ph.D. National Institute of Standards and Technology NIST Fellow & Special Assistant to the Director for Forensic Science Vice-Chair, National Commission on Forensic Science World Forensics Festival Seoul, Korea October 15, 2014 #12;Definition

272

Amplification of chromosomal DNA in situ  

DOE Patents [OSTI]

Amplification of chromosomal DNA in situ to increase the amount of DNA associated with a chromosome or chromosome region is described. The amplification of chromosomal DNA in situ provides for the synthesis of Fluorescence in situ Hybridization (FISH) painting probes from single dissected chromosome fragments, the production of cDNA libraries from low copy mRNAs and improved in Comparative Genomic Hybridization (CGH) procedures.

Christian, Allen T. (Tracy, CA); Coleman, Matthew A. (Livermore, CA); Tucker, James D. (Livermore, CA)

2002-01-01T23:59:59.000Z

273

DNA nanotubes and helical nanotapes via self-assembly of ssDNA-amphiphiles  

E-Print Network [OSTI]

DNA nanotubes and helical nanotapes via self- assembly of ssDNA-amphiphiles Timothy R. Pearcea and Efrosini Kokkoli*b DNA nanotubes were created using molecular self-assembly of single-stranded DNA (ss. The nanotube structures were formed by bilayers of amphiphiles, with the hydrophobic components forming

Kokkoli, Efie

274

Supplementary Information for: Integrating DNA Strand Displacement Circuitry with DNA Tile Self-assembly  

E-Print Network [OSTI]

Supplementary Information for: Integrating DNA Strand Displacement Circuitry with DNA Tile Self-assembly of Contents: · Supplementary Figures 1. Native polyacrylamide gel electrophoresis of DNA tile self-assembly 2. UV absorbance annealing and melting curves of DNA tile self-assembly 3. Characterization

Zhang, David Yu

275

Single-Molecule Study of DNA Polymerization Activity of HIV-1 Reverse Transcriptase on DNA Templates  

E-Print Network [OSTI]

Single-Molecule Study of DNA Polymerization Activity of HIV-1 Reverse Transcriptase on DNA unwinding protein, such as a helicase. In order to understand the mechanism of polymerization through secondary structures, we investigated the DNA polymerization activity of HIV-1 RT on long ssDNA templates

Xie, Xiaoliang Sunney

276

Physics and learning based computational models for breaking bow waves based on new boundary immersion approaches  

E-Print Network [OSTI]

A ship moving on the free surface produces energetic breaking bow waves which generate spray and air entrainment. Present experimental, analytic, and numerical studies of this problem are costly, inaccurate and not robust. ...

Weymouth, Gabriel David

2008-01-01T23:59:59.000Z

277

Electroweak symmetry breaking in the era of the Higgs boson discovery  

E-Print Network [OSTI]

In this thesis we study possible deviations from the electroweak symmetry breaking mechanism as predicted by the Standard Model (SM), and we introduce a new framework to analyze hadronic final states at colliders. In the ...

Bertolini, Daniele, Ph. D. Massachusetts Institute of Technology

2014-01-01T23:59:59.000Z

278

Introduction of Break-Out Session at the International PV Module Quality Assurance Forum (Presentation)  

SciTech Connect (OSTI)

This presentation outlines review requirements for quality assurance (QA) rating systems, logical design of QA systems, and specific tasks for break-out session 1 of the 2011 International PV Module Quality Assurance Forum.

Kurtz, S.; Wohlgemuth, J.; Yamamichi, M.; Sample, T.

2011-07-01T23:59:59.000Z

279

Double Ended Guillotine Break in a Prismatic Block VHTR Lower Plenum Air Ingress Scenario  

E-Print Network [OSTI]

The double ended guillotine break leading to density-driven air ingress has been identified as a low probability yet high consequence event for Very High Temperature Reactor (VHTR). The lower plenum of the VHTR contains the core support structure...

Hartley, Jessica

2012-10-19T23:59:59.000Z

280

E-Print Network 3.0 - anomaly-mediated supersymmetry breaking...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

; Physics 58 arXiv:hep-ph0103095v19Mar2001 Low-Energy Supersymmetry and its Phenomenology Summary: . This approach has been called anomaly-mediated SUSY-breaking (AMSB)....

Note: This page contains sample records for the topic "dna double-strand breaks" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


281

Spring Break Course in Florida! Forensic entomology is a branch of forensic sci-  

E-Print Network [OSTI]

Spring Break Course in Florida! Forensic entomology is a branch of forensic sci- ence utilizing the science of forensic en- tomology through daily laboratory and field ac- tivities. We also interact

282

Probe and method for DNA detection  

DOE Patents [OSTI]

A hybridization probe containing two linear strands of DNA lights up upon hybridization to a target DNA using silver nanoclusters that have been templated onto one of the DNA strands. Hybridization induces proximity between the nanoclusters on one strand and an overhang on the other strand, which results in enhanced fluorescence emission from the nanoclusters.

Yeh, Hsin-Chih; Werner, James Henry; Sharma, Jaswinder Kumar; Martinez, Jennifer Suzanne

2013-07-02T23:59:59.000Z

283

Self-assembled DNA Structures for Nanoconstruction  

E-Print Network [OSTI]

Self-assembled DNA Structures for Nanoconstruction Hao Yan, Peng Yin, Sung Ha Park, Hanying Li methods based on DNA self-assembly. Here we review our recent experimental progress to utilize novel DNA nanostructures for self-assembly as well as for templates in the fabrication of functional nano

Yin, Peng

284

HELICASE DEPENDENT AMPLICATION transfer the DNA  

E-Print Network [OSTI]

STEP 1 STEP 2 STEP 3 HELICASE DEPENDENT AMPLICATION HELICASE DNA POLYMERASE transfer the DNA instrumentation. MOLECULAR BIOMARKERS MADE UP DNA or RNA STRANDS ARE A POWERFUL WEAPON IN DETECTING DISEASE. USING for sample preparation at the point to care using only hand generated power.... #12;

285

Simulation of a small break loss of coolant accident conducted at the BETHSY Integral Test Facility  

E-Print Network [OSTI]

. The computer code RELAP5/MOD3 was used to model the BETHSY Integral Test Facility for a. small break loss of coolant accident. This transient simulates a 2 inch cold leg break without high pressure safety injection, following the conditions of International..., and general input to my gra, duate education. TABLE OF CONTENTS CHAPTER Page I INTRODUCTION I. 1 Need for Investigation I. 2 Computational Modeling . I. 3 Experimental Modeling I, 4 International Cooperation . 1 3 RELAP5 CODE DESCRIPTION II. 1...

Bott, Charles Patrick

1992-01-01T23:59:59.000Z

286

Microfluidic DNA sample preparation method and device  

DOE Patents [OSTI]

Manipulation of DNA molecules in solution has become an essential aspect of genetic analyses used for biomedical assays, the identification of hazardous bacterial agents, and in decoding the human genome. Currently, most of the steps involved in preparing a DNA sample for analysis are performed manually and are time, labor, and equipment intensive. These steps include extraction of the DNA from spores or cells, separation of the DNA from other particles and molecules in the solution (e.g. dust, smoke, cell/spore debris, and proteins), and separation of the DNA itself into strands of specific lengths. Dielectrophoresis (DEP), a phenomenon whereby polarizable particles move in response to a gradient in electric field, can be used to manipulate and separate DNA in an automated fashion, considerably reducing the time and expense involved in DNA analyses, as well as allowing for the miniaturization of DNA analysis instruments. These applications include direct transport of DNA, trapping of DNA to allow for its separation from other particles or molecules in the solution, and the separation of DNA into strands of varying lengths.

Krulevitch, Peter A. (Pleasanton, CA); Miles, Robin R. (Danville, CA); Wang, Xiao-Bo (San Diego, CA); Mariella, Raymond P. (Danville, CA); Gascoyne, Peter R. C. (Bellaire, TX); Balch, Joseph W. (Livermore, CA)

2002-01-01T23:59:59.000Z

287

Fleet DNA Project (Fact Sheet)  

SciTech Connect (OSTI)

The Fleet DNA Project - designed by the U.S. Department of Energy's National Renewable Energy Laboratory (NREL) in partnership with Oak Ridge National Laboratory - aims to accelerate the evolution of advanced vehicle development and support the strategic deployment of market-ready technologies that reduce costs, fuel consumption, and emissions. At the heart of the Fleet DNA Project is a clearinghouse of medium- and heavy-duty commercial fleet transportation data for optimizing the design of advanced vehicle technologies or for selecting a given technology to invest in. An easy-to-access online database will help vehicle manufacturers and fleets understand the broad operational range for many of today's commercial vehicle vocations.

Not Available

2012-10-01T23:59:59.000Z

288

Electromagnetic Signals from Bacterial DNA  

E-Print Network [OSTI]

Chemical reactions can be induced at a distance due to the propagation of electromagnetic signals during intermediate chemical stages. Although is is well known at optical frequencies, e.g. photosynthetic reactions, electromagnetic signals hold true for muck lower frequencies. In E. coli bacteria such electromagnetic signals can be generated by electric transitions between energy levels describing electrons moving around DNA loops. The electromagnetic signals between different bacteria within a community is a "wireless" version of intercellular communication found in bacterial communities connected by "nanowires". The wireless broadcasts can in principle be of both the AM and FM variety due to the magnetic flux periodicity in electron energy spectra in bacterial DNA orbital motions.

A. Widom; J. Swain; Y. N. Srivastava; S. Sivasubramanian

2012-02-09T23:59:59.000Z

289

Channel plate for DNA sequencing  

DOE Patents [OSTI]

This invention is a channel plate that facilitates data compaction in DNA sequencing. The channel plate has a length, a width and a thickness, and further has a plurality of channels that are parallel. Each channel has a depth partially through the thickness of the channel plate. Additionally an interface edge permits electrical communication across an interface through a buffer to a deposition membrane surface. 15 figs.

Douthart, R.J.; Crowell, S.L.

1998-01-13T23:59:59.000Z

290

Subcellular Spatial Correlation of Particle Traversal and Biological Response in Clinical Ion Beams  

SciTech Connect (OSTI)

Purpose: To report on the spatial correlation of physical track information (fluorescent nuclear track detectors, FNTDs) and cellular DNA damage response by using a novel hybrid detector (Cell-Fit-HD). Methods and Materials: The FNTDs were coated with a monolayer of human non-small cell lung carcinoma (A549) cells and irradiated with carbon ions (270.55 MeV u{sup ?1}, rising flank of the Bragg peak). Phosphorylated histone variant H2AX accumulating at the irradiation-induced double-strand break site was labeled (RIF). The position and direction of ion tracks in the FNTD were registered with the location of the RIF sequence as an ion track surrogate in the cell layer. Results: All RIF sequences could be related to their corresponding ion tracks, with mean deviations of 1.09 ?m and ?1.72 ?m in position and of 2.38° in slope. The mean perpendicular between ion track and RIF sequence was 1.58 ?m. The mean spacing of neighboring RIFs exhibited a regular rather than random spacing. Conclusions: Cell-Fit-HD allows for unambiguous spatial correlation studies of cell damage with respect to the intracellular ion traversal under therapeutic beam conditions.

Niklas, Martin, E-mail: m.niklas@dkfz.de [Division of Medical Physics in Radiation Oncology, German Cancer Research Center, Heidelberg (Germany); German Cancer Consortium, National Center for Radiation Research in Oncology, Heidelberg Institute of Radiation Oncology, Heidelberg (Germany); Abdollahi, Amir [German Cancer Consortium, National Center for Radiation Research in Oncology, Heidelberg Institute of Radiation Oncology, Heidelberg (Germany); Molecular and Translational Radiation Oncology, Heidelberg Ion-Beam Therapy Center, University of Heidelberg Medical School and National Center for Tumor Diseases, German Cancer Research Center, Heidelberg (Germany); Department of Radiation Oncology and Radiation Therapy, University Hospital Heidelberg, Heidelberg (Germany); Heidelberg Ion-Beam Therapy Center, Heidelberg (Germany); Akselrod, Mark S. [Stillwater Crystal Growth Division, Landauer Inc, Stillwater, Oklahoma (United States); Debus, Jürgen [German Cancer Consortium, National Center for Radiation Research in Oncology, Heidelberg Institute of Radiation Oncology, Heidelberg (Germany); Molecular and Translational Radiation Oncology, Heidelberg Ion-Beam Therapy Center, University of Heidelberg Medical School and National Center for Tumor Diseases, German Cancer Research Center, Heidelberg (Germany); Department of Radiation Oncology and Radiation Therapy, University Hospital Heidelberg, Heidelberg (Germany); Heidelberg Ion-Beam Therapy Center, Heidelberg (Germany); Jäkel, Oliver [Division of Medical Physics in Radiation Oncology, German Cancer Research Center, Heidelberg (Germany); German Cancer Consortium, National Center for Radiation Research in Oncology, Heidelberg Institute of Radiation Oncology, Heidelberg (Germany); Department of Radiation Oncology and Radiation Therapy, University Hospital Heidelberg, Heidelberg (Germany); Heidelberg Ion-Beam Therapy Center, Heidelberg (Germany); and others

2013-12-01T23:59:59.000Z

291

the Adaptive Response, Genetic Haplo-Insufficiency and Genomic Instability  

SciTech Connect (OSTI)

The linear no-threshold (LNT) hypothesis is the driving force in the establishment of radiation protection standards. However, the scientific basis for linearity has been brought into question, particularly due to the concerns about induced radiation resistance as it pertains to oxidative stress. Specifically, we investigated the observation that tumor hypoxia is associated with malignant progression, increased metastases, chemo- and radioresistance and poor prognosis. Experiments were conducted with non-malignant 3T3/NIH cells and normal human lung fibroblasts (NHLF) that were subjected to ?-irradiation under the levels of oxygen resembling those in growing tumors, and related our data to the concentrations of dissolved oxygen (DO), which is a better indicator of the amounts of residual oxygen inside the cells cultured in the hypoxic or anoxic atmosphere. We found that at DO levels about 0.5 mg/L cells subjected to both short-term (17 hours) and prolonged (48-72 hours) hypoxia continued to proliferate, and that apoptotic events were decreased at the early hours of hypoxic treatment. We showed that the short-term hypoxia up-regulated p53-binding protein 1 (53BP1) and resulted in facilitated 53BP1 nuclear foci formation and disappearance, thus indicating the higher efficiency of DNA double strand breaks repair processes. The latter was confirmed by the lower micronuclei incidence in irradiated hypoxic cells.

Geard, Charles R.

2014-12-12T23:59:59.000Z

292

Transgenic plants are sensitive bioindicators of nuclear pollution caused by the Chernobyl accident  

SciTech Connect (OSTI)

To evaluate the genetic consequences of radioactive contamination originating from the Nuclear reactor accident of Chernobyl on indigenous populations of plants and animals, it is essential to determine the rates of accumulating genetic changes in chronically irradiated populations. An increase in germline mutation rates in humans living close to the Chernobyl Nuclear Power Plant site, and a two- to tenfold increase in germline mutations in barn swallows breeding in Chernobyl have been reported. Little is known, however, about the effects of chronic irradiation on plant genomes. Ionizing radiation causes double-strand breaks in DNA, which are repaired via illegitimate or homologous recombination. The authors make use of Arabidopsis thaliana plants carrying a {beta}-glucuronidase marker gene as a recombination substrate to monitor genetic alterations in plant populations, which are caused by nuclear pollution of the environment around Chernobyl. A significant increase in somatic intrachromosomal recombination frequencies was observed at nuclear pollution levels from 0.1--900 Ci/km{sup 2}, consistent with an increase in chromosomal aberrations. This bioindicator may serve as a convenient and ethically acceptable alternative to animal systems.

Kovalchuk, I.; Kovalchuk, O. [Ivano-Frankivsk State Medical Academy (Ukraine)]|[Friedrich Miescher Inst., Basel (Switzerland); Arkhipov, A. [Chernobyl Scientific and Technical Center of International Research (Ukraine); Hohn, B. [Friedrich Miescher Inst., Basel (Switzerland)

1998-11-01T23:59:59.000Z

293

Quantitative Analysis of Clustered DNA Damages Induced by Silicon Beams of Different Kinetic Energy  

SciTech Connect (OSTI)

Humans may b exposed to highly energetic charged particle radiation as a result of medical treatments, occupational activitie or accidental events. In recent years, our increasing presence and burgeoning interest in space exploration beyond low Earth orbit has led to a large increase in the research of the biological effects ofcharged particle radiation typical of that encountered in the space radiation environment. The study of the effects of these types of radiation qualities in terms ofDNA damage induction and repair is fundamental to understand mechanisms both underlying their greater biological effectiveness as we)) as the short and long term risks of health effects such as carcinogenesis, degen rative diseases and premature aging. Charged particle radiation induces a variety of DNA alterations, notably bistranded clustered damages, defined as two or more closely-opposed strand break , oxidized bases or abasic sites within a few helical turns. The induction of such highly complex DNA damage enhances the probability of incorrect or incomplete repair and thus constitutes greater potential for genomic instability, cell death and transformation.

Keszenman D. J.; Keszenman, D.J.; Bennett, P.V.; Sutherland, B.M.; Wilson, P.F.

2013-05-14T23:59:59.000Z

294

The evolution of the break preclusion concept for nuclear power plants in Germany  

SciTech Connect (OSTI)

In the updating of the Guidelines for PWR`s of the {open_quotes}Reaktor-Sicherheitskommission{close_quotes} (RSK) in 1981 the requirements on the design have been changed with respect to the postulated leaks and breaks in the primary pressure boundary. The major change was a revision in the requirements for pipe whip protection. As a logical consequence of the {open_quotes}concept of basic safety{close_quotes} a guillotine type break or any other break type resulting in a large opening is not postulated any longer for the calculation of reaction and jet forces. As an upper limit for a leak an area of 0, 1 A (A = open cross section of the pipe) is postulated. This decision was based on a general assessment of the present PWR system design in Germany. Since then a number of piping systems have been requalified in the older nuclear power plants to comply with the break preclusion concept. Also a number of extensions of the concept have been developed to cover also leak-assumptions for branch pipes. Furthermore due considerations have been given to other aspects which could contribute to a leak development in the primary circuit, like vessel penetrations, manhole covers, flanges, etc. Now the break preclusion concept originally applied to the main piping has been developed into an integrated concept for the whole pressure boundary within the containment and will be applied also in the periodic safety review of present nuclear power plants.

Schulz, H. [Gesellschaft fuer Anlagen- und Reaktorsicherheit, Koeln (Germany)

1997-04-01T23:59:59.000Z

295

Effects of $?$-cluster breaking on 3$?$ cluster structures in $^{12}$C  

E-Print Network [OSTI]

To clarify the effects of $\\alpha$-cluster breaking on 3$\\alpha$ cluster structures in $^{12}$C, we investigate $^{12}$C using a hybrid model that combines the Brink-Bloch cluster model with the $p_{3/2}$ subshell closure wave function. We have found that $\\alpha$-cluster breaking caused by spin-orbit force significantly changes cluster structures of excited $0^{+}$ states through orthogonality to lower states. Spatially developed cluster components of the $0^{+}_{2}$ state are reduced. The $0^{+}_{3}$ state changes from a vibration mode in the bending motion of three $\\alpha$ clusters to a chain-like 3$\\alpha$ structure having an open triangle configuration. As a result of these structure changes of $0^{+}$ states, the band assignment for the $2^{+}_{2}$ state is changed by the $\\alpha$-cluster breaking effect. Namely, in model calculations without the $\\alpha$-cluster breaking effect, the $0^{+}_{2}$ state is assigned to be the band-head of the $2^{+}_{2}$ state. However, when we incorporate $\\alpha$-cluster breaking caused by the spin-orbit force, the $0^{+}_{3}$ state is regarded as the band-head of the $2^{+}_{2}$ state.

Tadahiro Suhara; Yoshiko Kanada-En'yo

2015-02-17T23:59:59.000Z

296

The dynamic interplay between DNA damage and metabolism : the metabolic fate and transport of DNA lesions and novel DNA damage derived from intermediary metabolism  

E-Print Network [OSTI]

The work presented in this thesis explores two novel and complementary facets of endogenous DNA damage: the development of biomarkers of inflammation based on metabolites of DNA damage products and the formation of DNA ...

Jumpathong, Watthanachai

2014-01-01T23:59:59.000Z

297

DNA  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmospheric Optical Depth7-1D: Vegetation Proposed Newcatalyst phases onOrganization FY Middle SchoolARM-TR-01468This4t c 5DMSEdrives

298

Enhancing the DNA Patent Database  

SciTech Connect (OSTI)

Final Report on Award No. DE-FG0201ER63171 Principal Investigator: LeRoy B. Walters February 18, 2008 This project successfully completed its goal of surveying and reporting on the DNA patenting and licensing policies at 30 major U.S. academic institutions. The report of survey results was published in the January 2006 issue of Nature Biotechnology under the title “The Licensing of DNA Patents by US Academic Institutions: An Empirical Survey.” Lori Pressman was the lead author on this feature article. A PDF reprint of the article will be submitted to our Program Officer under separate cover. The project team has continued to update the DNA Patent Database on a weekly basis since the conclusion of the project. The database can be accessed at dnapatents.georgetown.edu. This database provides a valuable research tool for academic researchers, policymakers, and citizens. A report entitled Reaping the Benefits of Genomic and Proteomic Research: Intellectual Property Rights, Innovation, and Public Health was published in 2006 by the Committee on Intellectual Property Rights in Genomic and Protein Research and Innovation, Board on Science, Technology, and Economic Policy at the National Academies. The report was edited by Stephen A. Merrill and Anne-Marie Mazza. This report employed and then adapted the methodology developed by our research project and quoted our findings at several points. (The full report can be viewed online at the following URL: http://www.nap.edu/openbook.php?record_id=11487&page=R1). My colleagues and I are grateful for the research support of the ELSI program at the U.S. Department of Energy.

Walters, LeRoy B.

2008-02-18T23:59:59.000Z

299

DNA-guided nanoparticle assemblies  

DOE Patents [OSTI]

In some embodiments, DNA-capped nanoparticles are used to define a degree of crystalline order in assemblies thereof. In some embodiments, thermodynamically reversible and stable body-centered cubic (bcc) structures, with particles occupying <.about.10% of the unit cell, are formed. Designs and pathways amenable to the crystallization of particle assemblies are identified. In some embodiments, a plasmonic crystal is provided. In some aspects, a method for controlling the properties of particle assemblages is provided. In some embodiments a catalyst is formed from nanoparticles linked by nucleic acid sequences and forming an open crystal structure with catalytically active agents attached to the crystal on its surface or in interstices.

Gang, Oleg; Nykypanchuk, Dmytro; Maye, Mathew; van der Lelie, Daniel

2013-07-16T23:59:59.000Z

300

Human Genome Research: Decoding DNA  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmospheric Optical Depth7-1D: Vegetation ProposedUsingFun withconfinement plasmas inPortalAllBPA addressesHowHughHumanDecoding DNA

Note: This page contains sample records for the topic "dna double-strand breaks" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


301

Assembling semiconductor nanocomposites using DNA replication technologies.  

SciTech Connect (OSTI)

Deoxyribonucleic acid (DNA) molecules represent Nature's genetic database, encoding the information necessary for all cellular processes. From a materials engineering perspective, DNA represents a nanoscale scaffold with highly refined structure, stability across a wide range of environmental conditions, and the ability to interact with a range of biomolecules. The ability to mass-manufacture functionalized DNA strands with Angstrom-level resolution through DNA replication technology, however, has not been explored. The long-term goal of the work presented in this report is focused on exploiting DNA and in vitro DNA replication processes to mass-manufacture nanocomposite materials. The specific objectives of this project were to: (1) develop methods for replicating DNA strands that incorporate nucleotides with ''chemical handles'', and (2) demonstrate attachment of nanocrystal quantum dots (nQDs) to functionalized DNA strands. Polymerase chain reaction (PCR) and primer extension methodologies were used to successfully synthesize amine-, thiol-, and biotin-functionalized DNA molecules. Significant variability in the efficiency of modified nucleotide incorporation was observed, and attributed to the intrinsic properties of the modified nucleotides. Noncovalent attachment of streptavidin-coated nQDs to biotin-modified DNA synthesized using the primer extension method was observed by epifluorescence microscopy. Data regarding covalent attachment of nQDs to amine- and thiol-functionalized DNA was generally inconclusive; alternative characterization tools are necessary to fully evaluate these attachment methods. Full realization of this technology may facilitate new approaches to manufacturing materials at the nanoscale. In addition, composite nQD-DNA materials may serve as novel recognition elements in sensor devices, or be used as diagnostic tools for forensic analyses. This report summarizes the results obtained over the course of this 1-year project.

Heimer, Brandon W.; Crown, Kevin K.; Bachand, George David

2005-11-01T23:59:59.000Z

302

Optical Recognition of Converted DNA Nucleotides for Single-Molecule DNA  

E-Print Network [OSTI]

Optical Recognition of Converted DNA Nucleotides for Single-Molecule DNA Sequencing Using Nanopore among individual nucleotides (nt). The system must be capable of differentiating among the four bases

303

Chemical biology of mutagenesis and DNA repair: cellular responses to DNA alkylation  

E-Print Network [OSTI]

The reaction of DNA-damaging agents with the genome results in a plethora of lesions, commonly referred to as adducts. Adducts may cause DNA to mutate, they may represent the chemical precursors of lethal events and they ...

Shrivastav, Nidhi

304

Nucleotide capacitance calculation for DNA sequencing  

SciTech Connect (OSTI)

Using a first-principles linear response theory, the capacitance of the DNA nucleotides, adenine, cytosine, guanine and thymine, are calculated. The difference in the capacitance between the nucleotides is studied with respect to conformational distortion. The result suggests that although an alternate current capacitance measurement of a single-stranded DNA chain threaded through a nano-gap electrodes may not sufficient to be used as a stand alone method for rapid DNA sequencing, the capacitance of the nucleotides should be taken into consideration in any GHz-frequency electric measurements and may also serve as an additional criterion for identifying the DNA sequence.

Lu, Jun-Qiang [ORNL; Zhang, Xiaoguang [ORNL

2008-01-01T23:59:59.000Z

305

Molecular Imaging of the ATM Kinase Activity  

SciTech Connect (OSTI)

Purpose: Ataxia telangiectasia mutated (ATM) is a serine/threonine kinase critical to the cellular DNA-damage response, including from DNA double-strand breaks. ATM activation results in the initiation of a complex cascade of events including DNA damage repair, cell cycle checkpoint control, and survival. We sought to create a bioluminescent reporter that dynamically and noninvasively measures ATM kinase activity in living cells and subjects. Methods and Materials: Using the split luciferase technology, we constructed a hybrid cDNA, ATM-reporter (ATMR), coding for a protein that quantitatively reports on changes in ATM kinase activity through changes in bioluminescence. Results: Treatment of ATMR-expressing cells with ATM inhibitors resulted in a dose-dependent increase in bioluminescence activity. In contrast, induction of ATM kinase activity upon irradiation resulted in a decrease in reporter activity that correlated with ATM and Chk2 activation by immunoblotting in a time-dependent fashion. Nuclear targeting improved ATMR sensitivity to both ATM inhibitors and radiation, whereas a mutant ATMR (lacking the target phosphorylation site) displayed a muted response. Treatment with ATM inhibitors and small interfering (si)RNA-targeted knockdown of ATM confirm the specificity of the reporter. Using reporter expressing xenografted tumors demonstrated the ability of ATMR to report in ATM activity in mouse models that correlated in a time-dependent fashion with changes in Chk2 activity. Conclusions: We describe the development and validation of a novel, specific, noninvasive bioluminescent reporter that enables monitoring of ATM activity in real time, in vitro and in vivo. Potential applications of this reporter include the identification and development of novel ATM inhibitors or ATM-interacting partners through high-throughput screens and in vivo pharmacokinetic/pharmacodynamic studies of ATM inhibitors in preclinical models.

Williams, Terence M. [Department of Radiation Oncology, Ohio State University, Columbus, Ohio (United States); Nyati, Shyam [Department of Radiation Oncology, University of Michigan Medical Center, Ann Arbor, Michigan (United States); Center for Molecular Imaging, University of Michigan Medical Center, Ann Arbor, Michigan (United States); Ross, Brian D. [Department of Radiation Oncology, University of Michigan Medical Center, Ann Arbor, Michigan (United States); Department of Radiology, University of Michigan Medical Center, Ann Arbor, Michigan (United States); Rehemtulla, Alnawaz, E-mail: alnawaz@umich.edu [Department of Radiation Oncology, University of Michigan Medical Center, Ann Arbor, Michigan (United States); Center for Molecular Imaging, University of Michigan Medical Center, Ann Arbor, Michigan (United States); Department of Radiology, University of Michigan Medical Center, Ann Arbor, Michigan (United States)

2013-08-01T23:59:59.000Z

306

Symmetry breaking patterns and collective modes of spin-one color superconductors  

E-Print Network [OSTI]

Spin-one color superconductor is a viable candidate phase of dense matter in the interiors of compact stars. Its low-energy excitations will influence the transport properties of such matter and thus have impact on late-stage evolution of neutron stars. It also provides a good example of spontaneous symmetry breaking with rich breaking patterns. In this contribution, we reanalyze the phase diagram of a spin-one color superconductor and point out that a part of it is occupied by noninert states, which have been neglected in literature so far. We classify the collective Nambu--Goldstone modes, which are essential to the transport phenomena.

Tomas Brauner; Jin-yi Pang; Qun Wang

2009-09-23T23:59:59.000Z

307

The concepts of leak before break and absolute reliability of NPP equipment and piping  

SciTech Connect (OSTI)

This paper describes the absolute reliability (AR) concept for ensuring safe operation of nuclear plant equipment and piping. The AR of a pipeline or component is defined as the level of reliability when the probability of an instantaneous double-ended break is near zero. AR analysis has been applied to Russian RBMK and VVER type reactors. It is proposed that analyses required for application of the leak before break concept should be included in AR implementation. The basic principles, methods, and approaches that provide the basis for implementing the AR concept are described.

Getman, A.F.; Komarov, O.V.; Sokov, L.M. [and others

1997-04-01T23:59:59.000Z

308

Semiclassical treatment of symmetry breaking and bifurcations in a non-integrable potential  

E-Print Network [OSTI]

We have derived an analytical trace formula for the level density of the H\\'enon-Heiles potential using the improved stationary phase method, based on extensions of Gutzwiller's semiclassical path integral approach. This trace formula has the correct limit to the standard Gutzwiller trace formula for the isolated periodic orbits far from all (critical) symmetry-breaking points. It continuously joins all critical points at which an enhancement of the semiclassical amplitudes occurs. We found a good agreement between the semi- classical and the quantum oscillating level densities for the gross shell structures and for the energy shell corrections, solving the symmetry breaking problem at small energies.

M. V. Koliesnik; Ya. D. Krivenko-Emetov; A. G. Magner; K. Arita; M. Brack

2014-11-10T23:59:59.000Z

309

Wave breaking phenomenon of lower-hybrid oscillations induced by a background inhomogeneous magnetic field  

SciTech Connect (OSTI)

In a fluid description, we study space-time evolution of lower hybrid modes in a cold quasi-neutral homogeneous plasma in presence of a background inhomogeneous magnetic field. Within a linear analysis, a dispersion relation with inhomogeneous magnetic field shows 'phase mixing' of such oscillations. A manifestation of 'phase mixing' is shown in 'mode coupling.' By using Lagrangian variables, an exact solution is presented in parametric form of this nonlinear time dependent problem. It is demonstrated that initially excited lower hybrid modes always break via phase mixing phenomenon in presence of an inhomogeneous magnetic field. Breaking of such oscillations is revealed by the appearance of spikes in the plasma density profile.

Maity, Chandan; Chakrabarti, Nikhil [Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata 700064 (India); Sengupta, Sudip [Institute for Plasma Research, Bhat, Gandhinagar 382428 (India)

2012-10-15T23:59:59.000Z

310

A DNA Based Implementation of an Evolutionary Search for Good Encodings for DNA Computation  

E-Print Network [OSTI]

lelism, capacity, and power. This potential, however, is limited by the constraints imposed by the DNA chemistry 1]. Adleman 2] introduced a way to solve combina- torial optimization problems with DNA- mental reaction in DNA based computation is hydro- gen bonding between Watson-Crick complement base pairs

Deaton, Russell J.

311

Staining Slides with Sybr Green Aime M. Dudley  

E-Print Network [OSTI]

Staining Slides with Sybr Green Aimée M. Dudley Sybr Green I is a double-stranded DNA dye slides at room temperature over 0.3 M potassium phosphate 30 min. 2. UV cross link on setting 1 large volumes of ddH2O 8. Wash 7 min. in Hexane 9. Scan Slide on Sybr Green protocol (Laser #4, Filter

Dudley, Aimee

312

Dynamics and control of DNA sequence amplification  

SciTech Connect (OSTI)

DNA amplification is the process of replication of a specified DNA sequence in vitro through time-dependent manipulation of its external environment. A theoretical framework for determination of the optimal dynamic operating conditions of DNA amplification reactions, for any specified amplification objective, is presented based on first-principles biophysical modeling and control theory. Amplification of DNA is formulated as a problem in control theory with optimal solutions that can differ considerably from strategies typically used in practice. Using the Polymerase Chain Reaction as an example, sequence-dependent biophysical models for DNA amplification are cast as control systems, wherein the dynamics of the reaction are controlled by a manipulated input variable. Using these control systems, we demonstrate that there exists an optimal temperature cycling strategy for geometric amplification of any DNA sequence and formulate optimal control problems that can be used to derive the optimal temperature profile. Strategies for the optimal synthesis of the DNA amplification control trajectory are proposed. Analogous methods can be used to formulate control problems for more advanced amplification objectives corresponding to the design of new types of DNA amplification reactions.

Marimuthu, Karthikeyan [Department of Chemical Engineering and Center for Advanced Process Decision-Making, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213 (United States); Chakrabarti, Raj, E-mail: raj@pmc-group.com, E-mail: rajc@andrew.cmu.edu [Department of Chemical Engineering and Center for Advanced Process Decision-Making, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213 (United States); Division of Fundamental Research, PMC Advanced Technology, Mount Laurel, New Jersey 08054 (United States)

2014-10-28T23:59:59.000Z

313

DNA Motif Representation with Nucleotide Dependency  

E-Print Network [OSTI]

DNA Motif Representation with Nucleotide Dependency Francis Chin1 and Henry Leung1 1 Department, these representations cannot model biological binding sites well because they fail to capture nucleotide interdependence. It has been pointed out by many researchers that the nucleotides of the DNA binding site cannot

Chin, Francis Y.L.

314

Carbon Nanotube DNA Sensor and Sensing Mechanism  

E-Print Network [OSTI]

Carbon Nanotube DNA Sensor and Sensing Mechanism Xiaowu Tang,*,, Sarunya Bansaruntip, Nozomi; Revised Manuscript Received June 7, 2006 ABSTRACT We report the fabrication of single-walled carbon nanotube (SWNT) DNA sensors and the sensing mechanism. The simple and generic protocol for label

Le Roy, Robert J.

315

Recombinant DNA encoding a desulfurization biocatalyst  

DOE Patents [OSTI]

This invention relates to a recombinant DNA molecule containing a gene or genes which encode a biocatalyst capable of desulfurizing a fossil fuel which contains organic sulfur molecules. For example, the present invention encompasses a recombinant DNA molecule containing a gene or genes of a strain of Rhodococcus rhodochrous. 13 figs.

Rambosek, J.; Piddington, C.S.; Kovacevich, B.R.; Young, K.D.; Denome, S.A.

1994-10-18T23:59:59.000Z

316

Electric DNA chips for determination of pathogenic microorganisms  

E-Print Network [OSTI]

Electric DNA chips for determination of pathogenic microorganisms Yanling Liu Doctoral thesis Liu (2008): Electric DNA chips for determination of pathogenic microorganisms. School of Biotechnology, Royal Institute of Technology (KTH), Stockholm, Sweden Abstract Silicon-based electric DNA chip arrays

Enfors, Sven-Olof

317

Mitochondrial DNA phylogenetic analysis of Myrmecolacidae (Insecta: Strepsiptera) va  

E-Print Network [OSTI]

Guinea are determined. Methods were developed for extraction, amplification, and sequencing of mitochondrial DNA (mtDNA) from Strepsiptera. High-resolution mtDNA sequences obtained by these methods were used in the phylogenetic analysis of Myrmecolacidae...

Halbert, Natalie Rose

2013-02-22T23:59:59.000Z

318

Single molecule analysis of DNA electrophoresis in microdevices  

E-Print Network [OSTI]

Given that current electrophoresis technology is inadequate for mapping large O[100 kilobasepair] DNA, several promising lab-on-chip designs for DNA mapping have been recently proposed that require either 1) a DNA molecule ...

Randall, Greg C

2006-01-01T23:59:59.000Z

319

Flow cytometric detection method for DNA samples  

DOE Patents [OSTI]

Disclosed herein are two methods for rapid multiplex analysis to determine the presence and identity of target DNA sequences within a DNA sample. Both methods use reporting DNA sequences, e.g., modified conventional Taqman.RTM. probes, to combine multiplex PCR amplification with microsphere-based hybridization using flow cytometry means of detection. Real-time PCR detection can also be incorporated. The first method uses a cyanine dye, such as, Cy3.TM., as the reporter linked to the 5' end of a reporting DNA sequence. The second method positions a reporter dye, e.g., FAM.TM. on the 3' end of the reporting DNA sequence and a quencher dye, e.g., TAMRA.TM., on the 5' end.

Nasarabadi,Shanavaz (Livermore, CA); Langlois, Richard G. (Livermore, CA); Venkateswaran, Kodumudi S. (Round Rock, TX)

2011-07-05T23:59:59.000Z

320

Atomic force microscopy of biochemically tagged DNA  

SciTech Connect (OSTI)

Small fragments of DNA of known length were made with the polymerase chain reaction. These fragments had biotin molecules covalently attached at their ends. They were subsequently labeled with a chimeric protein fusion between streptavidin and two immunoglobulin G-binding domains of staphyloccocal protein A. This tetrameric species was expected to bind up to four DNA molecules via their attached biotin moieties. The DNA-protein complex was deposited on mica and imaged with an atomic force microscope. The images revealed the protein chimera at the expected location at the ends of the strands of DNA as well as the expected dimers, trimers, and tetramers of DNA bound to a single protein. 25 refs., 5 figs.

Ogletree, D.F.; Kolbe, W.; Spengler, S.; Salmeron, M. (Lawrence Berkeley Laboratory, CA (United States)); Hansma, H.G.; Bezanilla, M. (Univ. of California, Santa Barbara (United States)); Sano, T.; Smith, C.S.; Cantor, C.R. (Boston Univ., MA (United States))

1993-05-01T23:59:59.000Z

Note: This page contains sample records for the topic "dna double-strand breaks" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


321

Storing data encoded DNA in living organisms  

DOE Patents [OSTI]

Current technologies allow the generation of artificial DNA molecules and/or the ability to alter the DNA sequences of existing DNA molecules. With a careful coding scheme and arrangement, it is possible to encode important information as an artificial DNA strand and store it in a living host safely and permanently. This inventive technology can be used to identify origins and protect R&D investments. It can also be used in environmental research to track generations of organisms and observe the ecological impact of pollutants. Today, there are microorganisms that can survive under extreme conditions. As well, it is advantageous to consider multicellular organisms as hosts for stored information. These living organisms can provide as memory housing and protection for stored data or information. The present invention provides well for data storage in a living organism wherein at least one DNA sequence is encoded to represent data and incorporated into a living organism.

Wong; Pak C. (Richland, WA), Wong; Kwong K. (Sugar Land, TX), Foote; Harlan P. (Richland, WA)

2006-06-06T23:59:59.000Z

322

Flow cytometric detection method for DNA samples  

DOE Patents [OSTI]

Disclosed herein are two methods for rapid multiplex analysis to determine the presence and identity of target DNA sequences within a DNA sample. Both methods use reporting DNA sequences, e.g., modified conventional Taqman.RTM. probes, to combine multiplex PCR amplification with microsphere-based hybridization using flow cytometry means of detection. Real-time PCR detection can also be incorporated. The first method uses a cyanine dye, such as, Cy3.TM., as the reporter linked to the 5' end of a reporting DNA sequence. The second method positions a reporter dye, e.g., FAM, on the 3' end of the reporting DNA sequence and a quencher dye, e.g., TAMRA, on the 5' end.

Nasarabadi, Shanavaz (Livermore, CA); Langlois, Richard G. (Livermore, CA); Venkateswaran, Kodumudi S. (Livermore, CA)

2006-08-01T23:59:59.000Z

323

Constituent Particle Break-up during Hot Rolling of AA 5182 Nicolas Moulin(1)  

E-Print Network [OSTI]

Constituent Particle Break-up during Hot Rolling of AA 5182 Nicolas Moulin(1) , Estelle Parra hot reversible rolling of AA5182 alloy sheets has been analysed. The sizes and shapes of intermetallic particles in as-cast and industrially hot rolled AA5182 alloys sheets were characterized by 3D X ray

Paris-Sud XI, Université de

324

Implications of different supersymmetry breaking patterns for the spectrum and decay of neutralinos and charginos  

SciTech Connect (OSTI)

We consider different patterns of supersymmetry breaking gaugino mass parameters, and implications of these patterns for the phenomenology of neutralinos and charginos in models of low-energy supersymmetry. We outline a general procedure for obtaining approximate values for the neutralino masses relevant for our analysis, and describe the constraints on the gaugino mass parameters which follow from the present experimental limits on the mass of the lightest chargino. We evaluate an upper bound on the mass of the lightest neutralino that follows from the structure of the mass matrix in the studied patterns. Using the experimental lower limit for the chargino mass, we examine the lower bound for the neutralino masses. Using a sum rule for the squared masses of charginos and neutralinos, we discuss how to distinguish between different models for the supersymmetry breaking. We discuss in detail the decay modes of neutralinos and charginos. Our analysis shows that by measuring the masses and decay properties of the neutralinos, one can distinguish between different patterns of supersymmetry breaking in the gaugino sector. We then compare the dark matter characteristics that arise in different models for the supersymmetry breaking gaugino mass parameters.

Huitu, Katri; Tiitola, Paavo [Department of Physics / Helsinki Institute of Physics, University of Helsinki, P.O. Box 64, FIN-00014 Helsinki (Finland); Laamanen, Jari [Theoretical High Energy Physics, Radboud University Nijmegen, P.O. Box 9010, NL-6500 GL Nijmegen (Netherlands); Institut fuer Physik, Technische Universitaet Dortmund, D-44221 Dortmund (Germany); Pandita, P. N. [Department of Physics, North Eastern Hill University, Shillong 793 022 (India)

2010-12-01T23:59:59.000Z

325

XXII ICTAM, 2529 August 2008, Adelaide, Australia SPACE-TIME MEASUREMENTS OF BREAKING WAVE KINEMATICS  

E-Print Network [OSTI]

with light intensity. WAVE KINEMATICS Phase average vorticity In order to have a statistical evolutionXXII ICTAM, 25­29 August 2008, Adelaide, Australia SPACE-TIME MEASUREMENTS OF BREAKING WAVE KINEMATICS AND VOID FRACTION IN THE SURF ZONE Olivier Kimmoun1 , 2 Hubert Branger1 1IRPHE, CNRS, Aix

Paris-Sud XI, Université de

326

Electronic Heating at High Bias in Atomic-Scale Au Break Junctions Ruoyu Chen,1  

E-Print Network [OSTI]

, U.S.A. Heating in nanoscale systems driven out of equilibrium is of fundamental importance, has O-10 Electronic Heating at High Bias in Atomic-Scale Au Break Junctions Ruoyu Chen,1, Texas, U.S.A. 2 Department of Electrical and Computer Engineering, Rice University, Houston, Texas 77005

327

Draft Final (13/6/01) Summary of Diagnostics Break Out Session at BPS Workshop 2  

E-Print Network [OSTI]

challenge, arguably the most difficult challenge ever undertaken in plasma diagnostics The challenge must wall and plasma measurements will be required to support a BPX; (ii) what is the status of diagnosticPage 1 Draft Final (13/6/01) Summary of Diagnostics Break Out Session at BPS Workshop 2 Prepared

328

A BreakEven Formulation for Evaluating Branch Predictor Energy Efficiency #  

E-Print Network [OSTI]

A Break­Even Formulation for Evaluating Branch Predictor Energy Efficiency # Michele Co, Dee A demonstrated that a better branch pre­ dictor can increase the energy­efficiency of the system, even if the new a simple, effective metric for eval­ uating the tradeoff between processor energy­efficiency and branch

Co, Michele

329

Observation of the Ankle and Evidence for a High-Energy Break in the Cosmic Ray  

E-Print Network [OSTI]

Observation of the Ankle and Evidence for a High-Energy Break in the Cosmic Ray Spectrum R known as "the ankle" near 3 Ã? 1018 eV, and strong evidence for a supression near 6 Ã? 1019 eV. 1

330

Total Break of the -IC Signature Scheme Pierre-Alain Fouque1  

E-Print Network [OSTI]

- analysis of multivariate schemes and have lead to the efficient break of SFLASH in practice. In this work- signature scheme recently proposed at PKC 2007. This cryptosystem is a multivariate scheme based on a new internal quadratic primitive which avoids some drawbacks of previous multivari- ate schemes: the scheme

Fouque, Pierre-Alain

331

Breaking POET Authentication with a Single Query Jian Guo, Jeremy Jean, Thomas Peyrin, and Lei Wang  

E-Print Network [OSTI]

Breaking POET Authentication with a Single Query Jian Guo, J´er´emy Jean, Thomas Peyrin, and Lei article, we describe a very practical and simple attack on the au- thentication part of POET authenticated encryption mode proposed at FSE 2014. POET is a provably secure scheme that was designed to resist various

332

Trends and breaks in per-capita carbon dioxide emissions, 1870-2028  

E-Print Network [OSTI]

We consider per-capita carbon dioxide emission trends in 16 early developed countries over the period 1870-2028. Using a multiple-break time series method we find more evidence for very early downturns in per-capita trends ...

Lanne, Markku

2003-01-01T23:59:59.000Z

333

Instability of two-dimensional graphene: Breaking sp2 bonds with soft x rays  

E-Print Network [OSTI]

Instability of two-dimensional graphene: Breaking sp2 bonds with soft x rays S. Y. Zhou,1,2 Ç. Ö the stability of various kinds of graphene samples under soft x-ray irradiation. Our results show that in single-layer exfoliated graphene a closer analog to two-dimensional material , the in-plane carbon- carbon bonds

Zettl, Alex

334

Facebook, Twitter and Google Plus for Breaking News: Is there a winner? Miles Osborne  

E-Print Network [OSTI]

Facebook, Twitter and Google Plus for Breaking News: Is there a winner? Miles Osborne School Media have be- gun to carry news. Here we examine how Facebook, Google Plus and Twitter report Facebook or Google Plus. Face- book and Google Plus largely repost newswire stories and their main research

Osborne, Miles

335

All Your Face Are Belong to Us: Breaking Facebook's Social Authentication  

E-Print Network [OSTI]

All Your Face Are Belong to Us: Breaking Facebook's Social Authentication Iasonas Polakis FORTH adversaries from compromising accounts using stolen credentials. Facebook has recently released a two can obtain the information needed to solve the chal- lenges presented by Facebook. We implement

Yang, Junfeng

336

Multiple Structural Breaks in India's GDP: Evidence from India's Service Sector  

E-Print Network [OSTI]

of economists and policy makers. India was designated as an agricultural country with a highest share1 Multiple Structural Breaks in India's GDP: Evidence from India's Service Sector Purba Roy Choudhury1 Abstract: This paper takes a comprehensive investigation into India's service sector, the main

Bandyopadhyay, Antar

337

Assessing the catastrophic break up of Briksdalsbreen, Norway,1 associated with rapid climate change.2  

E-Print Network [OSTI]

about the potential influence of rapid climate change13 on the stability of major ice sheets retreat of Greenland's tidewater glaciers.32 33 Introduction34 The response of glaciers to climate change1 Assessing the catastrophic break up of Briksdalsbreen, Norway,1 associated with rapid climate

Southampton, University of

338

Studying the Breaking Mechanism of Polymer-Based In-Situ Gelled Acids using Solid Breaker  

E-Print Network [OSTI]

of this study is to examine the working mechanisms of the solid breaker and the factors that affect the breaking time. The flowback of the spent acid and the core damage induced by it will also be assessed in detail. Viscosity and rheological experimental...

Tian, Zhida

2012-10-19T23:59:59.000Z

339

Partial degeneracy breaking of the hydrogen energy spectrum from su_q(2)  

E-Print Network [OSTI]

In this work we investigate the q-deformation of the so(4) dynamical symmetry of the hydrogen atom using the theory of the quantum group su_q(2), and construct the discrete part of the energy spectrum. This will lead to a partial breaking of the degeneracy of the energy levels and to a reduction of the Hilbert space.

P. G. Castro; R. Kullock

2012-12-07T23:59:59.000Z

340

NHMFL Breaks the 100 Tesla Barrier Gregory S. Boebinger, National High Magnetic Field Laboratory  

E-Print Network [OSTI]

NHMFL Breaks the 100 Tesla Barrier Gregory S. Boebinger, National High Magnetic Field Laboratory. 109no. 31 12404-12407 On March 22nd 2012, the NHMFL ­ Pulsed Field Facility broke the 100T tesla barrier, setting a world record of 100.75 tesla for a non-destructive magnet. By using advanced

Weston, Ken

Note: This page contains sample records for the topic "dna double-strand breaks" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


341

Winery waste makes fuel Electricity, bacteria break organics in wastewater into hydrogen gas  

E-Print Network [OSTI]

MSNBC.com Winery waste makes fuel Electricity, bacteria break organics in wastewater into hydrogen method for generating hydrogen fuel from wastewater is now operating at a California winery material in the wastewater into hydrogen gas. There is a lot more energy locked in the wastewater than

342

Numerical Simulation of Breaking Waves Using Level-Set Navier-Stokes Method  

E-Print Network [OSTI]

) equations were employed for the prediction of nonlinear wave-interaction and wave-breaking phenomena over sloping beaches. In the level-set finite-analytic Navier-Stokes (FANS) method, the free surface is represented by the zero level-set function...

Dong, Qian

2010-07-14T23:59:59.000Z

343

Mathematical model and simulation of gas ow through a porous medium in high breaking capacity  

E-Print Network [OSTI]

Mathematical model and simulation of gas #29;ow through a porous medium in high breaking capacity, France. Abstract. A one-dimensional model is introduced to describe the gas #29;ow and the heat transfer model coupled with a porous medium model taking into account the mechanical interaction gas-silica sand

Sart, Remi

344

Role of global warming on the statistics of record-breaking temperatures S. Redner1,  

E-Print Network [OSTI]

Role of global warming on the statistics of record-breaking temperatures S. Redner1, * and Mark R in Philadelphia, as a function of the number of years of observation. We then consider the case of global warming question arises: is global warming the cause of such heat waves or are they merely statistical fluctuations

Redner, Sidney

345

Symmetry-breaking thermally induced collapse of dipolar Bose-Einstein condensates Andrej Junginger,  

E-Print Network [OSTI]

Symmetry-breaking thermally induced collapse of dipolar Bose-Einstein condensates Andrej Junginger LE11 3TU, UK (Dated: August 13, 2012) We investigate a Bose-Einstein condensate with additional long" that mediates the collapse of the condensate. For a certain value of the s-wave scatting length our

346

Application of a Downhole Flowmeter to Detecting Casing Breaks in a Geothermal Well  

SciTech Connect (OSTI)

The downhole flowmeter logging system for high temperature geothermal wells developed at Wairakei, New Zealand, is proving to be an invaluable tool for use during workovers to repair or reinstate problem wells. This contribution describes a straightforward example of identifying breaks in plain casing near the top of a productive well.

Syms, Margot C.; Syms, Peter H.; Bixley, Paul F.

1980-12-16T23:59:59.000Z

347

The University of New Hampshire in 2020: Breaking Silos, Transforming Lives, Reimagining the University  

E-Print Network [OSTI]

their historic mission and respond energetically and effectively to these new challenges? The University of New1 The University of New Hampshire in 2020: Breaking Silos, Transforming Lives, Reimagining of our state, our nation and our world. For 143 years we have served the people and communities of New

348

University Of Kentucky Supercomputer Breaks The $1,000 Per GFLOPS Barrier  

E-Print Network [OSTI]

University Of Kentucky Supercomputer Breaks The $1,000 Per GFLOPS Barrier May 22, 2000, Lexington, Kentucky May 2000, Lexington, Kentucky: Researchers at the University of Kentucky have constructed that cost down to about $3,000 per GFLOPS. KLAT2, Kentucky Linux Athlon Testbed 2 (http

Dietz, Henry G. "Hank"

349

Crystal Structure of the Chromodomain Helicase DNA-binding Protein 1 (Chd1) DNA-binding Domain in Complex with DNA  

SciTech Connect (OSTI)

Chromatin remodelers are ATP-dependent machines that dynamically alter the chromatin packaging of eukaryotic genomes by assembling, sliding, and displacing nucleosomes. The Chd1 chromatin remodeler possesses a C-terminal DNA-binding domain that is required for efficient nucleosome sliding and believed to be essential for sensing the length of DNA flanking the nucleosome core. The structure of the Chd1 DNA-binding domain was recently shown to consist of a SANT and SLIDE domain, analogous to the DNA-binding domain of the ISWI family, yet the details of how Chd1 recognized DNA were not known. Here we present the crystal structure of the Saccharomyces cerevisiae Chd1 DNA-binding domain in complex with a DNA duplex. The bound DNA duplex is straight, consistent with the preference exhibited by the Chd1 DNA-binding domain for extranucleosomal DNA. Comparison of this structure with the recently solved ISW1a DNA-binding domain bound to DNA reveals that DNA lays across each protein at a distinct angle, yet contacts similar surfaces on the SANT and SLIDE domains. In contrast to the minor groove binding seen for Isw1 and predicted for Chd1, the SLIDE domain of the Chd1 DNA-binding domain contacts the DNA major groove. The majority of direct contacts with the phosphate backbone occur only on one DNA strand, suggesting that Chd1 may not strongly discriminate between major and minor grooves.

Sharma A.; Heroux A.; Jenkins K. R.; Bowman G. D.

2011-12-09T23:59:59.000Z

350

Hydrogen Bond Networks: Structure and Evolution after Hydrogen Bond Breaking John B. Asbury, Tobias Steinel, and M. D. Fayer*  

E-Print Network [OSTI]

Hydrogen Bond Networks: Structure and Evolution after Hydrogen Bond Breaking John B. Asbury, TobiasVed: September 1, 2003; In Final Form: December 18, 2003 The nature of hydrogen bonding networks following hydrogen bond breaking is investigated using vibrational echo correlation spectroscopy of the hydroxyl

Fayer, Michael D.

351

Effects of intermittent entrainment of air bubbles by breaking wind waves on ocean reflectance and underwater light field  

E-Print Network [OSTI]

Effects of intermittent entrainment of air bubbles by breaking wind waves on ocean reflectance, 2000]. The intermittent nature of air entrainment by breaking waves manifests itself as dramatic, Polish Academy of Sciences, Sopot, Poland Abstract. Light-scattering properties of air bubbles suspended

Stramski, Dariusz

352

ap dna endonuclease: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

specificity Raines, Ronald T. 2 A DNA and restriction enzyme implementation of Turing Ma (Turing machines; Universal Turing machines; recombinant DNA; nonpalindromic...

353

adenovirus dna sequences: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

description of the given DNA string in terms of a smaller set of distinct domain labels. This yields a minimal domain description of a given DNA sequence, significantly...

354

Minor Groove Deformability of DNA: A Molecular Dynamics Free...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Minor Groove Deformability of DNA: A Molecular Dynamics Free EnergySimulation Study. Minor Groove Deformability of DNA: A Molecular Dynamics Free EnergySimulation Study. Abstract:...

355

Electrochemical Branched-DNA Assay for Polymerase Chain Reaction...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Branched-DNA Assay for Polymerase Chain Reaction-Free Detection and Quantification of Oncogenes in Messenger RNA Electrochemical Branched-DNA Assay for Polymerase Chain...

356

adapting dna microarray: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Gene Expression Analysis & R Tutorial 12;DNA Microarrays An R Tutorial Functional Genomics Qiu, Weigang 3 Research tools known as DNA microarrays are Biology and Medicine...

357

archived dna microarrays: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Gene Expression Analysis & R Tutorial 12;DNA Microarrays An R Tutorial Functional Genomics Qiu, Weigang 3 Research tools known as DNA microarrays are Biology and Medicine...

358

Protein Bridges DNA Base and Nucleotide Excision Repair Pathways  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

a nucleotide from the DNA double helix to its active site to access damaged nucleotides. But unlike AGT and most other known DNA nucleotide-flipping proteins, this...

359

Constraint of DNA on Functionalized Graphene Improves Its Biostability...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Constraint of DNA on Functionalized Graphene Improves Its Biostability and Specificity. Constraint of DNA on Functionalized Graphene Improves Its Biostability and Specificity....

360

Method for in vitro recombination  

SciTech Connect (OSTI)

The present invention relates to an in vitro method, using isolated protein reagents, for joining two double-stranded (ds) DNA molecules of interest, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule share a region of sequence identity. The method allows the joining of a number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes. It can be used, e.g., to join synthetically produced sub-fragments of a gene or genome of interest.

Gibson, Daniel Glenn; Smith, Hamilton O

2013-05-07T23:59:59.000Z

Note: This page contains sample records for the topic "dna double-strand breaks" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


361

Method of quantitating dsDNA  

DOE Patents [OSTI]

A method for quantitating dsDNA in an aqueous sample solution containing an unknown amount of dsDNA. A first aqueous test solution containing a known amount of a fluorescent dye-dsDNA complex and at least one fluorescence-attenutating contaminant is prepared. The fluorescence intensity of the test solution is measured. The first test solution is diluted by a known amount to provide a second test solution having a known concentration of dsDNA. The fluorescence intensity of the second test solution is measured. Additional diluted test solutions are similarly prepared until a sufficiently dilute test solution having a known amount of dsDNA is prepared that has a fluorescence intensity that is not attenuated upon further dilution. The value of the maximum absorbance of this solution between 200-900 nanometers (nm), referred to herein as the threshold absorbance, is measured. A sample solution having an unknown amount of dsDNA and an absorbance identical to that of the sufficiently dilute test solution at the same chosen wavelength is prepared. Dye is then added to the sample solution to form the fluorescent dye-dsDNA-complex, after which the fluorescence intensity of the sample solution is measured and the quantity of dsDNA in the sample solution is determined. Once the threshold absorbance of a sample solution obtained from a particular environment has been determined, any similarly prepared sample solution taken from a similar environment and having the same value for the threshold absorbance can be quantified for dsDNA by adding a large excess of dye to the sample solution and measuring its fluorescence intensity.

Stark, Peter C. (Los Alamos, NM); Kuske, Cheryl R. (Los Alamos, NM); Mullen, Kenneth I. (Los Alamos, NM)

2002-01-01T23:59:59.000Z

362

DNA fragment sizing and sorting by laser-induced fluorescence  

DOE Patents [OSTI]

A method is provided for sizing DNA fragments using high speed detection systems, such as flow cytometry to determine unique characteristics of DNA pieces from a sample. In one characterization the DNA piece is fragmented at preselected sites to produce a plurality of DNA fragments. The DNA piece or the resulting DNA fragments are treated with a dye effective to stain stoichiometrically the DNA piece or the DNA fragments. The fluorescence from the dye in the stained fragments is then examined to generate an output functionally related to the number of nucleotides in each one of the DNA fragments. In one embodiment, the intensity of the fluorescence emissions from each fragment is linearly related to the fragment length. The distribution of DNA fragment sizes forms a characterization of the DNA piece for use in forensic and research applications.

Hammond, Mark L. (Angier, NC); Jett, James H. (Los Alamos, NM); Keller, Richard A. (Los Alamos, NM); Marrone, Babetta L. (Los Alamos, NM); Martin, John C. (Los Alamos, NM)

1996-01-01T23:59:59.000Z

363

Sequential addition of short DNA oligos in DNA-polymerase-based synthesis reactions  

DOE Patents [OSTI]

A method of preselecting a multiplicity of DNA sequence segments that will comprise the DNA molecule of user-defined sequence, separating the DNA sequence segments temporally, and combining the multiplicity of DNA sequence segments with at least one polymerase enzyme wherein the multiplicity of DNA sequence segments join to produce the DNA molecule of user-defined sequence. Sequence segments may be of length n, where n is an odd integer. In one embodiment the length of desired hybridizing overlap is specified by the user and the sequences and the protocol for combining them are guided by computational (bioinformatics) predictions. In one embodiment sequence segments are combined from multiple reading frames to span the same region of a sequence, so that multiple desired hybridizations may occur with different overlap lengths.

Gardner, Shea N; Mariella, Jr., Raymond P; Christian, Allen T; Young, Jennifer A; Clague, David S

2013-06-25T23:59:59.000Z

364

Linear Thermodynamics of Rodlike DNA Filtration  

E-Print Network [OSTI]

Linear thermodynamics transportation theory is employed to study filtration of rodlike DNA molecules. Using the repeated nanoarray consisting of alternate deep and shallow regions, it is demonstrated that the complex ...

Li, Zirui

365

Intriguing DNA Editor Has a Structural Trigger  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

generating a lot of excitement because, unlike earlier genome-editing tools, its basic architecture doesn't have to be rebuilt each time a new section of DNA is targeted; it...

366

The Initiation of Bacterial DNA Replication  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

that the ATP-DnaA active site is in a closed configuration, which allows it to bind nucleotides using conserved residues from neighboring AAA+ protomers. The precise geometry of...

367

Deoxyribose oxidation chemistry and endogenous DNA adducts  

E-Print Network [OSTI]

Endogenous and exogenous oxidants react with cellular macromolecules to generate a variety of electrophiles that react with DNA produce cytotoxic and mutagenic adducts. One source of such electrophiles is deoxyribose in ...

Zhou, Xinfeng

2006-01-01T23:59:59.000Z

368

Unraveling siRNA Unzipping Kinetics with Graphene  

E-Print Network [OSTI]

Using all atom molecular dynamics simulations, we report spontaneous unzipping and strong binding of small interfering RNA (siRNA) on graphene. Our dispersion corrected density functional theory based calculations suggest that nucleosides of RNA have stronger attractive interactions with graphene as compared to DNA residues. These stronger interactions force the double stranded siRNA to spontaneously unzip and bind to the graphene surface. Unzipping always nucleates at one end of the siRNA and propagates to the other end after few base-pairs get unzipped. While both the ends get unzipped, the middle part remains in double stranded form because of torsional constraint. Unzipping probability distributions fitted to single exponential function give unzipping time (t) of the order of few nanoseconds which decrease exponentially with temperature. From the temperature variation of unzipping time we estimate the energy barrier to unzipping.

Santosh Mogurampelly; Swati Panigrahi; Dhananjay Bhattacharyya; A. K. Sood; Prabal K. Maiti

2012-07-19T23:59:59.000Z

369

Study of charge symmetry breaking in dd collisions with WASA-at-COSY  

SciTech Connect (OSTI)

Charge symmetry is an approximate symmetry of the strong interaction. Studies of its breaking can yield information on the u and d quark mass difference. A theoretical collaboration is currently working on the description of charge symmetry breaking mechanisms for dd{yields}{alpha}{pi}{sup 0} and np{yields}d{pi}{sup 0} within Chiral Perturbation Theory, using the data from TRI-UMF and IUCF. One of the items in the program of the WASA-at-COSY collaboration is to extend the data base for the dd{yields}{alpha}{pi}{sup 0} reaction to higher energies, which would allow the extraction of the information on the p-wave. Status of the analysis of experimental data along with the preliminary results from the pilot run will be presented here.

Wronska, Aleksandra [Institute of Physics, Jagiellonian University, Cracow, Reymonta 4, 30-059 Cracow (Poland)

2011-10-24T23:59:59.000Z

370

A Free Energy Landscape for Cage Breaking of Three Hard Disks  

E-Print Network [OSTI]

We investigate cage breaking in dense hard disk systems using a model of three Brownian disks confined within a circular corral. This system has a six-dimensional configuration space, but can be equivalently thought to explore a symmetric one-dimensional free energy landscape containing two energy minima separated by an energy barrier. The exact free energy landscape can be calculated as a function of system size. Results of simulations show the average time between cage breaking events follows an Arrhenius scaling when the energy barrier is large. We also discuss some of the consequences of using a one-dimensional representation to understand dynamics in a multi-dimensional space, such as diffusion acquiring spatial dependence and discontinuities in spatial derivatives of free energy.

Gary L. Hunter; Eric R. Weeks

2011-12-21T23:59:59.000Z

371

Breaking of relativistically intense longitudinal space charge waves: A description using Dawson sheet model  

SciTech Connect (OSTI)

Spatio-temporal evolution of relativistically intense longitudinal space charge waves in a cold homogeneous plasma is studied analytically as well as numerically, as an initial value problem, using Dawson sheet model. It is found that, except for very special initial conditions which generates the well known longitudinal Akhiezer-Polovin mode, for all other initial conditions, the waves break through a novel mechanism called phase mixing at an amplitude well below the Akhiezer-Polovin limit. An immediate consequence of this is, that Akhiezer-Polovin waves break when subjected to arbitrarily small longitudinal perturbations. We demonstrate this by performing extensive numerical simulations. This result may be of direct relevance to ultrashort, ultraintense laser/beam pulse-plasma interaction experiments where relativistically intense waves are routinely excited.

Sengupta, Sudip, E-mail: sudip@ipr.res.in [Institute for Plasma Research, Bhat , Gandhinagar - 382428 (India)

2014-02-11T23:59:59.000Z

372

RecA acts in trans to allow replication of damaged DNA by DNA polymerase V  

E-Print Network [OSTI]

, Michael M. Cox2 , Roger Woodgate3 & Myron F. Goodman1 The DNA polymerase V (pol V) and RecA proteins

Cox, Michael M.

373

Design of Autonomous DNA Cellular Automata , Sudheer Sahu  

E-Print Network [OSTI]

Design of Autonomous DNA Cellular Automata Peng Yin ¡ , Sudheer Sahu ¡ , Andrew J. Turberfield cellular computing devices, i.e. autonomous nano-mechanical DNA computing devices embedded in DNA lattices. As a prototype of such devices, we recently reported the design of an Autonomous DNA Turing Machine, which

Yin, Peng

374

Recombinant DNA Research in Human Subjects Duke Institutional Biosafety Committee  

E-Print Network [OSTI]

/2010 Summary. All Duke research using recombinant DNA (rDNA) in human subjects must comply with the NIH Guidelines for Research involving Recombinant DNA Molecules (available at http://oba.od.nih.gov/rdna/nih Biosafety Committee (IBC) [NIH Guidelines Section III-C and III-C-1]. The NIH defines rDNA broadly

375

Numerical Analysis of the Internal Kinematics and Dynamics of Three-dimensional Breaking Waves on B. Biausser, S.T. Grilli, P. Frauni  

E-Print Network [OSTI]

Numerical Analysis of the Internal Kinematics and Dynamics of Three-dimensional Breaking Waves/Navier-Stokes solver. Analysis of wave profiles and kinematics (velocity, vorticity, pressure) are carried out. Keyword the breaking and post-breaking in a three- dimensional numerical wave tank of a solitary wave over a sloping

Grilli, Stéphan T.

376

Watching Hydrogen Bonds Break: A Transient Absorption Study of Water Tobias Steinel, John B. Asbury, Junrong Zheng, and M. D. Fayer*  

E-Print Network [OSTI]

Watching Hydrogen Bonds Break: A Transient Absorption Study of Water Tobias Steinel, John B. Asbury of picoseconds, observe hydrogen bond breaking and monitor the equilibration of the hydrogen bond network in water. In addition, the vibrational lifetime, the time constant for hydrogen bond breaking, and the rate

Fayer, Michael D.

377

Isospin symmetry breaking and baryon-isospin correlations from Polyakov$-$Nambu$-$Jona-Lasinio model  

E-Print Network [OSTI]

We present a study of the 1+1 flavor system of strongly interacting matter in terms of the Polyakov$-$Nambu$-$Jona-Lasinio model. We find that though the small isospin symmetry breaking brought in through unequal light quark masses is too small to affect the thermodynamics of the system in general, it may have significant effect in baryon-isospin correlations and have a measurable impact in heavy-ion collision experiments.

Abhijit Bhattacharyya; Sanjay K. Ghosh; Anirban Lahiri; Sarbani Majumder; Sibaji Raha; Rajarshi Ray

2014-06-11T23:59:59.000Z

378

On the breaking and restoration of symmetries within the nuclear energy density functional formalism  

E-Print Network [OSTI]

We review the notion of symmetry breaking and restoration within the frame of nuclear energy density functional methods. We focus on key differences between wave-function- and energy-functional-based methods. In particular, we point to difficulties encountered within the energy functional framework and discuss new potential constraints on the underlying energy density functional that could make the restoration of broken symmetries better formulated within such a formalism. We refer to Ref.~\\cite{duguet10a} for details.

T. Duguet; J. Sadoudi

2010-10-19T23:59:59.000Z

379

Breaking Up (Hydrogen) No Longer As Hard To Do | Department of Energy  

Energy Savers [EERE]

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious RankCombustion |Energy UsageAUDITVehiclesTankless orA BRIEFApril 2015CommerceDepartmentBlowerBreaking Up

380

New Supersoft Supersymmetry Breaking Operators and a Solution to the $?$ Problem  

E-Print Network [OSTI]

We propose the framework, "generalized supersoft supersymmetry breaking". "Supersoft" models, with $D$-type supersymmetry breaking and heavy Dirac gauginos, are considerably less constrained by the LHC searches than the well studied MSSM. These models also ameliorate the supersymmetric flavor and $CP$ problems. However, previously considered mechanisms for obtaining a natural size Higgsino mass parameter (namely, $\\mu$) in supersoft models have been relatively complicated and contrived. Obtaining a $125\\gev$ for the mass of the lightest Higgs boson has also been difficult. Additional issues with the supersoft scenario arise from the fact that these models contain new scalars in the adjoint representation of the standard model, which may obtain negative squared-masses, breaking color and generating too large a $T$-parameter. In this work we introduce new operators into supersoft models which can potentially solve all these issues. A novel feature of this framework is that the new $\\mu$-term can give unequal masses to the up and down type Higgs fields, and the Higgsinos can be much heavier than the Higgs boson without fine-tuning. However, unequal Higgs and Higgsino masses can also destroy some attractive features of supersoft susy.

Ann E. Nelson; Tuhin S. Roy

2015-01-20T23:59:59.000Z

Note: This page contains sample records for the topic "dna double-strand breaks" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


381

Light scalar as the messenger of electroweak and flavor symmetry breaking  

SciTech Connect (OSTI)

We propose a new framework for understanding the hierarchies of fermion masses and mixings. The masses and mixings of all standard model (SM) charged fermions other than top arise from higher dimensional operators involving a messenger scalar S and flavon scalars F{sub i}. The flavons spontaneously break SM flavor symmetries at around the TeV scale. The SM singlet scalar S couples directly to the Higgs H and spontaneously breaks another U(1) at the electroweak scale. At the TeV scale, SM quarks and charged leptons have renormalizable couplings to S, but not to H or F{sub i}. These couplings involve new heavy vectorlike fermions. Integrating out these fermions produces a pattern of higher dimensional operators that reproduce the observed hierarchies of the SM masses and mixings in terms of powers of the 'little hierarchy': the ratio of the electroweak scale to the flavor-breaking scale. The framework has important phenomenological implications. Flavor-changing neutral currents are within experimental limits but D{sup 0}-D{sup 0} mixing and B{sub s}{yields}{mu}{sup +}{mu}{sup -} could be close to current sensitivities. The neutral scalar s of the messenger field mixes with the light Higgs of the SM, which can have strong effects on Higgs decay branching fractions. The s mass eigenstate may be lighter than the Higgs, and could be detected at the Tevatron or the LHC.

Lykken, J. D. [Theoretical Physics Department, Fermilab, P.O. Box 500, Batavia, Illinois 60510 (United States); Murdock, Z. [Department of Physics and Oklahoma Center for High Energy Physics, Oklahoma State University, Stillwater, Oklahoma 74078 (United States); Nandi, S. [Theoretical Physics Department, Fermilab, P.O. Box 500, Batavia, Illinois 60510 (United States); Department of Physics and Oklahoma Center for High Energy Physics, Oklahoma State University, Stillwater, Oklahoma 74078 (United States)

2009-04-01T23:59:59.000Z

382

Canonical Gauge Coupling Unification in the Standard Model with High-Scale Supersymmetry Breaking  

E-Print Network [OSTI]

Inspired by the string landscape and the unified gauge coupling relation in the F-theory Grand Unified Theories (GUTs) and GUTs with suitable high-dimensional operators, we study the canonical gauge coupling unification and Higgs boson mass in the Standard Model (SM) with high-scale supersymmetry breaking. In the SM with GUT-scale supersymmetry breaking, we achieve the gauge coupling unification at about 5.3 x 10^{13} GeV, and the Higgs boson mass is predicted to range from 130 GeV to 147 GeV. In the SM with supersymmetry breaking scale from 10^4 GeV to 5.3 x 10^{13} GeV, gauge coupling unification can always be realized and the corresponding GUT scale M_U is from 10^{16} GeV to 5.3 x 10^{13} GeV, respectively. Also, we obtain the Higgs boson mass from 114.4 GeV to 147 GeV. Moreover, the discrepancies among the SM gauge couplings at the GUT scale are less than about 4-6%. Furthermore, we present the SU(5) and SO(10) models from the F-theory model building and orbifold constructions, and show that we do not have the dimension-five and dimension-six proton decay problems even if M_U \\le 5 x 10^{15} GeV.

Yun-Jie Huo; Tianjun Li; Dimitri V. Nanopoulos

2011-08-24T23:59:59.000Z

383

Head/tail Breaks for Visualization of City Structure and Dynamics  

E-Print Network [OSTI]

The things surrounding us vary dramatically, which implies that there are far more small things than large ones, e.g., far more small cities than large ones in the world. This dramatic variation is often referred to as fractal or scaling. To better reveal the fractal or scaling structure, a new classification scheme, namely head/tail breaks, has been developed to recursively derive different classes or hierarchical levels. The head/tail breaks works as such: divide things into a few large ones in the head (those above the average) and many small ones (those below the average) in the tail, and recursively continue the dividing process for the large ones (or the head) until the notion of far more small things than large ones has been violated. This paper attempts to argue that head/tail breaks can be a powerful visualization tool for illustrating structure and dynamics of natural cities. Natural cities refer to naturally or objectively defined human settlements based on a meaningful cutoff averaged from a massi...

Jiang, Bin

2015-01-01T23:59:59.000Z

384

IDENTIFYING BREAKS AND CURVATURE IN THE FERMI SPECTRA OF BRIGHT FLAT SPECTRUM RADIO QUASARS  

SciTech Connect (OSTI)

Knowing the site of {gamma}-ray emission in active galactic nucleus jets will do much for our understanding of the physics of the source. In particular, if the emission region is close to the black hole then absorption of {gamma}-rays with photons from the broad-line region could become significant. Such absorption is predicted to produce two specific spectral breaks in the {gamma}-ray spectra of Flat Spectrum Radio Quasars (FSRQs). We test this hypothesis using three years of Fermi observations of nine bright FSRQs. A simple power-law fit to the spectrum of each source can be significantly improved by introducing a break, but the break energies are inconsistent with those predicted by the double-absorber model. In some cases the fit can be further improved by a log-parabola. In addition, by dividing the data from each source into two equal epochs we find that the best description of an object's spectrum often varies between a log-parabola and a broken power law.

Harris, J.; Daniel, M. K.; Chadwick, P. M., E-mail: j.d.harris@durham.ac.uk [Department of Physics, Durham University, South Road, Durham DH1 3LE (United Kingdom)

2012-12-10T23:59:59.000Z

385

Simulation of a main steam line break with steam generator tube rupture using trace  

SciTech Connect (OSTI)

A simulation of the OECD/NEA ROSA-2 Project Test 5 was made with the thermal-hydraulic code TRACE5. Test 5 performed in the Large Scale Test Facility (LSTF) reproduced a Main Steam Line Break (MSLB) with a Steam Generator Tube Rupture (SGTR) in a Pressurized Water Reactor (PWR). The result of these simultaneous breaks is a depressurization in the secondary and primary system in loop B because both systems are connected through the SGTR. Good approximation was obtained between TRACE5 results and experimental data. TRACE5 reproduces qualitatively the phenomena that occur in this transient: primary pressure falls after the break, stagnation of the pressure after the opening of the relief valve of the intact steam generator, the pressure falls after the two openings of the PORV and the recovery of the liquid level in the pressurizer after each closure of the PORV. Furthermore, a sensitivity analysis has been performed to know the effect of varying the High Pressure Injection (HPI) flow rate in both loops on the system pressures evolution. (authors)

Gallardo, S.; Querol, A.; Verdu, G. [Departamento de Ingenieria Quimica Y Nuclear, Universitat Politecnica de Valencia, Camino de Vera s/n, 46022, Valencia (Spain)

2012-07-01T23:59:59.000Z

386

Breaking the Ice: Navigation in the Arctic Grace Xingxin Gao, Liang Heng, Todd Walter, and Per Enge  

E-Print Network [OSTI]

Breaking the Ice: Navigation in the Arctic Grace Xingxin Gao, Liang Heng, Todd Walter, and Per Enge (ION) Early Achievement Award. Liang Heng is a Ph.D. candidate under the guidance of Professor Per Enge

Gao, Grace Xingxin

387

TITAN code development for application to a PWR steam line break accident : final report 1983-1984  

E-Print Network [OSTI]

Modification of the TITAN computer code which enables it to be applied to a PWR steam line break accident has been accomplished. The code now has the capability of simulating an asymmetric inlet coolant temperature transient ...

Tsai, Chon-Kwo

1984-01-01T23:59:59.000Z

388

Mass-related inversion symmetry breaking and phonon self-energy renormalization in isotopically labeled AB-stacked bilayer graphene  

E-Print Network [OSTI]

A mass-related symmetry breaking in isotopically labeled bilayer graphene (2LG) was investigated during in-situ electrochemical charging of AB stacked (AB-2LG) and turbostratic (t-2LG) layers. The overlap of the two ...

Araujo, Paulo Antonio Trinidade

389

Introduction of Break-Out Session 2 of the 2011 International PV Module Quality Assurance Forum(Presentation)  

SciTech Connect (OSTI)

This presentation outlines the goals and specific tasks of break-out session 2 of the 2011 International PV Module Quality Assurance Forum, along with a review of accelerated stress tests used for photovoltaics (PV).

Wohlgemuth, J.; Kurtz, S.; Sample, T.; Yamamichi, M.

2011-07-01T23:59:59.000Z

390

Search for Supersymmetry with Gauge-Mediated Breaking in Diphoton Events with Missing Transverse Energy at CDF II  

E-Print Network [OSTI]

We present the results of a search for supersymmetry with gauge-mediated breaking and ?˜[subscript 1][superscript 0]??G˜ in the ??+missing transverse energy final state. In 2.6±0.2??fb[superscript -1] of pp? collisions ...

Bauer, Gerry P.

391

Break-Even Cost for Residential Solar Water Heating in the United States: Key Drivers and Sensitivities  

SciTech Connect (OSTI)

This paper examines the break-even cost for residential rooftop solar water heating (SWH) technology, defined as the point where the cost of the energy saved with a SWH system equals the cost of a conventional heating fuel purchased from the grid (either electricity or natural gas). We examine the break-even cost for the largest 1,000 electric and natural gas utilities serving residential customers in the United States as of 2008. Currently, the break-even cost of SWH in the United States varies by more than a factor of five for both electricity and natural gas, despite a much smaller variation in the amount of energy saved by the systems (a factor of approximately one and a half). The break-even price for natural gas is lower than that for electricity due to a lower fuel cost. We also consider the relationship between SWH price and solar fraction and examine the key drivers behind break-even costs. Overall, the key drivers of the break-even cost of SWH are a combination of fuel price, local incentives, and technical factors including the solar resource location, system size, and hot water draw.

Cassard, H.; Denholm, P.; Ong, S.

2011-02-01T23:59:59.000Z

392

Optical selection and collection of DNA fragments  

DOE Patents [OSTI]

Optical selection and collection of DNA fragments. The present invention includes the optical selection and collection of large (>.mu.g) quantities of clonable, chromosome-specific DNA from a sample of chromosomes. Chromosome selection is based on selective, irreversible photoinactivation of unwanted chromosomal DNA. Although more general procedures may be envisioned, the invention is demonstrated by processing chromosomes in a conventional flow cytometry apparatus, but where no droplets are generated. All chromosomes in the sample are first stained with at least one fluorescent analytic dye and bonded to a photochemically active species which can render chromosomal DNA unclonable if activated. After passing through analyzing light beam(s), unwanted chromosomes are irradiated using light which is absorbed by the photochemically active species, thereby causing photoinactivation. As desired chromosomes pass this photoinactivation point, the inactivating light source is deflected by an optical modulator; hence, desired chromosomes are not photoinactivated and remain clonable. The selection and photoinactivation processes take place on a microsecond timescale. By eliminating droplet formation, chromosome selection rates 50 times greater than those possible with conventional chromosome sorters may be obtained. Thus, usable quantities of clonable DNA from any source thereof may be collected.

Roslaniec, Mary C. (Los Alamos, NM); Martin, John C. (Los Alamos, NM); Jett, James H. (Los Alamos, NM); Cram, L. Scott (Los Alamos, NM)

1998-01-01T23:59:59.000Z

393

DNA nanotechnology: understanding and optimisation through simulation  

E-Print Network [OSTI]

DNA nanotechnology promises to provide controllable self-assembly on the nanoscale, allowing for the design of static structures, dynamic machines and computational architectures. In this article I review the state-of-the art of DNA nanotechnology, highlighting the need for a more detailed understanding of the key processes, both in terms of theoretical modelling and experimental characterisation. I then consider coarse-grained models of DNA, mesoscale descriptions that have the potential to provide great insight into the operation of DNA nanotechnology if they are well designed. In particular, I discuss a number of nanotechnological systems that have been studied with oxDNA, a recently developed coarse-grained model, highlighting the subtle interplay of kinetic, thermodynamic and mechanical factors that can determine behaviour. Finally, new results highlighting the importance of mechanical tension in the operation of a two-footed walker are presented, demonstrating that recovery from an unintended `overstepped' configuration can be accelerated by three to four orders of magnitude by application of a moderate tension to the walker's track. More generally, the walker illustrates the possibility of biasing strand-displacement processes to affect the overall rate.

Thomas E. Ouldridge

2014-11-07T23:59:59.000Z

394

THE IMPACT OF BARS ON DISK BREAKS AS PROBED BY S{sup 4}G IMAGING  

SciTech Connect (OSTI)

We have analyzed the radial distribution of old stars in a sample of 218 nearby face-on disks, using deep 3.6 {mu}m images from the Spitzer Survey of Stellar Structure in Galaxies. In particular, we have studied the structural properties of those disks with a broken or down-bending profile. We find that, on average, disks with a genuine single-exponential profile have a scale length and a central surface brightness which are intermediate to those of the inner and outer components of a down-bending disk with the same total stellar mass. In the particular case of barred galaxies, the ratio between the break and the bar radii (R{sub br}/R{sub bar}) depends strongly on the total stellar mass of the galaxy. For galaxies more massive than 10{sup 10} M{sub Sun }, the distribution is bimodal, peaking at R{sub br}/R{sub bar} {approx} 2 and {approx}3.5. The first peak, which is the most populated one, is linked to the outer Lindblad resonance of the bar, whereas the second one is consistent with a dynamical coupling between the bar and the spiral pattern. For galaxies below 10{sup 10} M{sub Sun }, breaks are found up to {approx}10 R{sub bar}, but we show that they could still be caused by resonances given the rising nature of rotation curves in these low-mass disks. While not ruling out star formation thresholds, our results imply that radial stellar migration induced by non-axisymmetric features can be responsible not only for those breaks at {approx}2 R{sub bar}, but also for many of those found at larger radii.

Munoz-Mateos, Juan Carlos; Sheth, Kartik; Kim, Taehyun [National Radio Astronomy Observatory/NAASC, 520 Edgemont Road, Charlottesville, VA 22903 (United States); Gil de Paz, Armando [Departamento de Astrofisica, Universidad Complutense de Madrid, E-28040 Madrid (Spain); Meidt, Sharon [Max-Planck-Institut fuer Astronomie, Koenigstuhl 17, D-69117 Heidelberg (Germany); Athanassoula, E.; Bosma, Albert [Aix Marseille Universite, CNRS, LAM (Laboratoire d'Astrophysique de Marseille) UMR 7326, F-13388 Marseille (France); Comeron, Sebastien; Laine, Jarkko; Laurikainen, Eija [Department of Physical Sciences/Astronomy Division, University of Oulu, FIN-90014 (Finland); Elmegreen, Debra M. [Department of Physics and Astronomy, Vassar College, Poughkeepsie, NY 12604 (United States); Elmegreen, Bruce G. [IBM Research Division, T.J. Watson Research Center, Yorktown Hts., NY 10598 (United States); Erroz-Ferrer, Santiago; Knapen, Johan H. [Instituto de Astrofisica de Canarias, E-38205 La Laguna (Spain); Gadotti, Dimitri A. [European Southern Observatory, Casilla 19001, Santiago 19 (Chile); Hinz, Joannah L. [University of Arizona, 933 N. Cherry Ave, Tucson, AZ 85721 (United States); Ho, Luis C.; Madore, Barry F. [The Observatories, Carnegie Institution of Washington, 813 Santa Barbara Street, Pasadena, CA 91101 (United States); Holwerda, Benne [European Space Agency, ESTEC, Keplerlaan 1, 2200-AG, Noordwijk (Netherlands); Jarrett, Thomas H., E-mail: jmunoz@nrao.edu [Astrophysics, Cosmology and Gravity Centre, Department of Astronomy, University of Cape Town, Private Bag X3, Rondebosch 7701 (South Africa); and others

2013-07-01T23:59:59.000Z

395

The role of quantum recurrence in superconductivity, carbon nanotubes and related gauge symmetry breaking  

E-Print Network [OSTI]

Pure quantum phenomena are characterized by intrinsic recurrences in space and time. We use such an intrinsic periodicity as a quantization condition to derive the essential phenomenology of superconductivity. The resulting description is based on fundamental quantum dynamics and geometrical considerations, rather than on microscopical characteristics of the superconducting materials. This allows for the interpretation of the related gauge symmetry breaking by means of the competition between quantum recurrence and thermal noise. We also test the validity of this approach to describe the case of carbon nanotubes.

Donatello Dolce; Andrea Perali

2014-09-29T23:59:59.000Z

396

Non-destructive qualification tests for ITER cryogenic axial insulating breaks  

SciTech Connect (OSTI)

In the ITER superconducting magnets the dielectric separation between the CICC (Cable-In-Conduit Conductors) and the helium supply pipes is made through the so-called insulating breaks (IB). These devices shall provide the required dielectric insulation at a 30 kV level under different types of stresses and constraints: thermal, mechanical, dielectric and ionizing radiations. As part of the R and D program, the ITER Organization launched contracts with industrial companies aimed at the qualification of the manufacturing techniques. After reviewing the main functional aspects, this paper describes and discusses the protocol established for non-destructive qualification tests of the prototypes.

Kosek, Jacek [Wroclaw University of Technology, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland and CERN, Geneva 23,CH-1211 (Switzerland); Lopez, Roberto; Tommasini, Davide [CERN, Geneva 23,CH-1211 (Switzerland); Rodriguez-Mateos, Felix [CERN, Geneva 23,CH-1211, Switzerland and ITER Organization, Route de Vinon sur Verdon, 13115 Saint Paul lez Durance (France)

2014-01-29T23:59:59.000Z

397

A probabilistic method for leak-before-break analysis of CANDU reactor pressure tubes  

SciTech Connect (OSTI)

A probabilistic code for the prediction of the cumulative probability of pressure tube ruptures in CANDU type reactors is described. Ruptures are assumed to result from the axial growth by delayed hydride cracking. The BLOOM code models the major phenomena that affect crack length and critical crack length during the reactor sequence of events following the first indications of leakage. BLOOM can be used to develop unit-specific estimates of the actual probability of pressure rupture in operating CANDU reactors and supplement the existing leak before break analysis.

Puls, M.P.; Wilkins, B.J.S.; Rigby, G.L. [Whiteshell Labs., Pinawa (Canada)] [and others

1997-04-01T23:59:59.000Z

398

TACIS 91: Application of leak-before-break concept in VVER 440-230  

SciTech Connect (OSTI)

The applicability of the leak-before-break (LBB) concept for primary piping in the first generation of WWER type plants in Russia is investigated. The procedures for LBB behavior used in France and Germany are applied, and the evaluation is discussed within the framework of the European Technical Assistance for the Community of Independent States (TACIS) project. Emphasis is placed on experimental validation of national and international engineering practice for evaluating and optimizing existing installations. Design criteria of WWER plants are compared to western standard design.

Bartholome, G.; Faidy, C.; Franco, C. [and others

1997-04-01T23:59:59.000Z

399

Charge independence, charge symmetry breaking in the S-wave nucleon-nucleon interaction, and renormalization  

SciTech Connect (OSTI)

We study the interplay between charge symmetry breaking and renormalization in the NN system for S-waves. We find a set of universality relations which disentangle explicitly the known long distance dynamics from low energy parameters and extend them to the Coulomb case. We analyze within such an approach the One-Boson-Exchange potential and the theoretical conditions which allow to relate the proton-neutron, proton-proton and neutron-neutron scattering observables without the introduction of extra new parameters and providing good phenomenological success.

Alvaro Calle Cordon,Manuel Pavon Valderrama,Enrique Ruiz Arriola

2012-02-01T23:59:59.000Z

400

Generalized mass formula for non-strange and hyper nuclei with SU(6) symmetry breaking  

E-Print Network [OSTI]

A simultaneous description of non-strange nuclei and hypernuclei is provided by a single mass formula inspired by the spin-flavour SU(6) symmetry breaking. This formula is used to estimate the hyperon binding energies of Lambda, double Lambda, Sigma, Cascade and Theta hypernuclei. The results are found to be in good agreement with the available experimental data on 'bound' nuclei and relativistic as well as quark mean field calculations. This mass formula is useful to estimate binding energies over a wide range of masses including the light mass nuclei. It is not applicable for repulsive potential.

C. Samanta; P. Roy Chowdhury; D. N. Basu

2006-01-19T23:59:59.000Z

Note: This page contains sample records for the topic "dna double-strand breaks" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


401

Universal routes to spontaneous PT-symmetry breaking in non-Hermitian quantum systems  

SciTech Connect (OSTI)

PT-symmetric systems can have a real spectrum even when their Hamiltonian is non-Hermitian, but develop a complex spectrum when the degree of non-Hermiticity increases. Here we utilize random-matrix theory to show that this spontaneous PT-symmetry breaking can occur via two distinct mechanisms, whose predominance is associated to different universality classes. Present optical experiments fall into the orthogonal class, where symmetry-induced level crossings render the characteristic absorption rate independent of the coupling strength between the symmetry-related parts of the system.

Schomerus, Henning [Department of Physics, Lancaster University, Lancaster LA1 4YB (United Kingdom)

2011-03-15T23:59:59.000Z

402

Replica Symmetry Breaking in the Intensity Fluctuation Overlap of Random Laser Emission Spectra  

E-Print Network [OSTI]

We report about a newly introduced overlap parameter of intensity fluctuations of waves in random media with arbitrary amount of disorder and non-linearity and its relationship to the replica theory overlap in the $2+4$ spherical complex spin-glass model. Symmetry breaking in the intensity fluctuation overlap is shown to be equivalent to the one occurring in the complex amplitude overlap, providing an easily verifiable test in typical experimental setups. The relevance of this order parameter is considered in describing the laser transition in random media and in explaining its glassy nature in terms of emission spectra data. The theoretical analysis is compared to recent measurements.

Antenucci, Fabrizio; Leuzzi, Luca

2015-01-01T23:59:59.000Z

403

NNSA Breaks Ground on Tritium Facilities at SRS | National Nuclear Security  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr May JunDatastreamsmmcrcalgovInstrumentsrucLas Conchas recovery challengeMultiscaleLogos NERSCJeffreyKeyAdministration Breaks Ground on

404

Breaking Ground and Driving Discovery | U.S. DOE Office of Science (SC)  

Office of Science (SC) Website

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmosphericNuclear SecurityTensile Strain Switched5 Industrial CarbonArticlesHumanJune 2008 Basic EnergyB.Bette Korber, 2004Breaking

405

EECBG Success Story: Breaking Ground in Miami-Dade | Department of Energy  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE:YearRound-UpHeat Pump Models |Conduct,Final9: DraftPlant,Community' | Department ofSensorsBreaking

406

Bubbles Help Break Energy Storage Record for Lithium Air-Batteries  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr May JunDatastreamsmmcrcalgovInstrumentsruc DocumentationP-Series to someone6Energy, science, and technology for theBubbles Help Break

407

Novel Thermal Break with Simplified Manufacturing for R7 Commercial Windows  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious RankCombustion | Department ofT ib l L d F S iPartnershipEnergy| Department of Energy Thermal Break

408

Role of DNA repair protein ERCC1 in skin cancer   

E-Print Network [OSTI]

Nucleotide excision repair (NER) is one of the major repair systems for removal of DNA lesions. The NER pathway has evolved mainly to repair UV-induced DNA damage and is also active against a broad range of endogenously ...

Song, Liang

2009-01-01T23:59:59.000Z

409

DNA hybridization : fundamental studies and applications in directed assembly  

E-Print Network [OSTI]

Programmed self-assembly using non-covalent DNA-DNA interactions is a promising technique for the creation of next-generation functional devices for electronic, optical, and magnetic applications. This thesis develops the ...

Bajaj, Manish G. (Manish Gopal)

2005-01-01T23:59:59.000Z

410

Analysis of the structural changes caused by positive DNA supercoiling  

E-Print Network [OSTI]

The procession of helix-tracking enzymes along a DNA molecule results in the formation of supercoils in the DNA, with positive supercoiling (overwinding) generated ahead of the enzyme, and negative supercoiling (underwinding) ...

Barth, Marita Christine

2007-01-01T23:59:59.000Z

411

Lectin cDNA and transgenic plants derived therefrom  

DOE Patents [OSTI]

Transgenic plants containing cDNA encoding Gramineae lectin are described. The plants preferably contain cDNA coding for barley lectin and store the lectin in the leaves. The transgenic plants, particularly the leaves exhibit insecticidal and fungicidal properties.

Raikhel, Natasha V. (Okemos, MI)

2000-10-03T23:59:59.000Z

412

The biological and metabolic fates of endogenous DNA damage products  

E-Print Network [OSTI]

DNA and other biomolecules are subjected to damaging chemical reactions during normal physiological processes and in states of pathophysiology caused by endogenous and exogenous mechanisms. In DNA, this damage affects ...

Chan, Wan Simon

413

Flow cytometric measurement of total DNA and incorporated halodeoxyuridine  

DOE Patents [OSTI]

A method for the simultaneous flow cylometric measurement of total cellular DNA content and of the uptake of DNA precursors as a measure of DNA synthesis during various phases of the cell cycle in normal and malignant cells in vitro and in vivo is described. The method comprises reacting cells with labelled halodeoxyuridine (HdU), partially denaturing cellular DNA, adding to the reaction medium monoclonal antibodies (mabs) reactive with HdU, reacting the bound mabs with a second labelled antibody, incubating the mixture with a DNA stain, and measuring simultaneously the intensity of the DNA stain as a measure of the total cellular DNA and the HdU incorporated as a measure of DNA synthesis. (ACR)

Dolbeare, F.A.; Gray, J.W.

1983-10-18T23:59:59.000Z

414

Defining functional DNA elements in the human genome  

E-Print Network [OSTI]

With the completion of the human genome sequence, attention turned to identifying and annotating its functional DNA elements. As a complement to genetic and comparative genomics approaches, the Encyclopedia of DNA Elements ...

Kellis, Manolis

415

Single cell trapping and DNA damage analysis using microwell arrays  

E-Print Network [OSTI]

With a direct link to cancer, aging, and heritable diseases as well as a critical role in cancer treatment, the importance of DNA damage is well-established. The intense interest in DNA damage in applications ranging from ...

Wood, David

416

A model for sample stacking in microcapillary DNA electrophoresis  

E-Print Network [OSTI]

Sanger's method of chain termination is the method of choice in DNA sequencing, where electrophoresis is used to separate the different sized DNA. In the past decade, microfabricated capillary devices have been developed ...

Srivastava, Alok Kumar, 1967-

2002-01-01T23:59:59.000Z

417

Photoelectrochemical array platform for genomic scale DNA synthesis  

E-Print Network [OSTI]

Molecular and synthetic biologists have increasing demand for large, high-fidelity constructs of synthetic DNA. Recent developments in harvesting oligonucleotides from DNA microarrays has proven that these can be assembled ...

Emig, Christopher Joseph

2006-01-01T23:59:59.000Z

418

Bayesian models for DNA microarray data analysis  

E-Print Network [OSTI]

as to perform future predictions. The method is applied to cancer classi?cation via cDNA microarrays. In particular, the genes BRCA1 and BRCA2 are associated with a hereditary disposition to breast cancer, and the method is used to identify the set of signi...

Lee, Kyeong Eun

2005-08-29T23:59:59.000Z

419

THE KINEMATICS OF IONIZED GAS IN LYMAN-BREAK ANALOGS AT z {approx} 0.2  

SciTech Connect (OSTI)

We present results for 19 'Lyman-break analogs' observed with Keck/OSIRIS with an adaptive-optics-assisted spatial resolution of less than 200 pc. We detect satellites/companions, diffuse emission, and velocity shear, all with high signal-to-noise ratios. These galaxies present remarkably high velocity dispersion along the line of sight ({approx}70 km s{sup -1}), much higher than standard star-forming spirals in the low-redshift universe. We artificially redshift our data to z {approx} 2.2 to allow for a direct comparison with observations of high-z Lyman-break galaxies and find striking similarities between both samples. This suggests that either similar physical processes are responsible for their observed properties, or, alternatively, that it is very difficult to distinguish between different mechanisms operating in the low- versus high-redshift starburst galaxies based on the available data. The comparison between morphologies in the UV/optical continuum and our kinemetry analysis often shows that neither is by itself sufficient to confirm or completely rule out the contribution from recent merger events. We find a correlation between the kinematic properties and stellar mass, in that more massive galaxies show stronger evidence for a disk-like structure. This suggests a co-evolutionary process between the stellar mass buildup and the formation of morphological and dynamical substructure within the galaxy.

Goncalves, Thiago S.; Martin, D. Christopher; Wyder, Ted K. [California Institute of Technology, MC 278-17, Pasadena, CA 91125 (United States); Basu-Zych, Antara [NASA Goddard Space Flight Center, Laboratory for X-ray Astrophysics, Greenbelt, MD 20771 (United States); Overzier, Roderik [Max-Planck-Institut fuer Astrophysik, D-85748 Garching (Germany); Law, David R.; Mallery, Ryan; Rich, R. Michael [Department of Physics and Astronomy, Division of Astronomy and Astrophysics, University of California, Los Angeles, CA 90095 (United States); Schiminovich, David [Department of Astronomy, Columbia University, MC 2457, 550 West 120th Street, New York, NY 10027 (United States); Heckman, Timothy H., E-mail: tsg@astro.caltech.ed [Department of Physics and Astronomy, The Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218 (United States)

2010-12-01T23:59:59.000Z

420

Leak before break evaluation for main steam piping system made of SA106 Gr.C  

SciTech Connect (OSTI)

The basis of the leak before break (LBB) concept is to demonstrate that piping will leak significantly before a double ended guillotine break (DEGB) occurs. This is demonstrated by quantifying and evaluating the leak process and prescribing safe shutdown of the plant on the basis of the monitored leak rate. The application of LBB for power plant design has reduced plant cost while improving plant integrity. Several evaluations employing LBB analysis on system piping based on DEGB design have been completed. However, the application of LBB on main steam (MS) piping, which is LBB applicable piping, has not been performed due to several uncertainties associated with occurrence of steam hammer and dynamic strain aging (DSA). The objective of this paper is to demonstrate the applicability of the LBB design concept to main steam lines manufactured with SA106 Gr.C carbon steel. Based on the material properties, including fracture toughness and tensile properties obtained from the comprehensive material tests for base and weld metals, a parametric study was performed as described in this paper. The PICEP code was used to determine leak size crack (LSC) and the FLET code was used to perform the stability assessment of MS piping. The effects of material properties obtained from tests were evaluated to determine the LBB applicability for the MS piping. It can be shown from this parametric study that the MS piping has a high possibility of design using LBB analysis.

Yang, Kyoung Mo; Jee, Kye Kwang; Pyo, Chang Ryul; Ra, In Sik [Korea Power Engineering Company, Seoul (Korea, Republic of)

1997-04-01T23:59:59.000Z

Note: This page contains sample records for the topic "dna double-strand breaks" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


421

DNA Strands Attached Inside Single Conical Nanopores: Ionic Pore Characteristics and Insight into DNA Biophysics  

E-Print Network [OSTI]

Kathawalla et al. 1989; Heng et al. 2005; Keyser et al.due to the electric ?eld (Heng et al. 2005; Randall et al.DNA analysis. Nanomedicine Heng JB, Aksimentiev A, Ho C,

Nguyen, Gael; Howorka, Stefan; Siwy, Zuzanna S.

2011-01-01T23:59:59.000Z

422

Orientational Dynamics and Dye-DNA Interactions in a Dye-Labeled DNA Aptamer  

E-Print Network [OSTI]

We report the picosecond and nanosecond timescale rotational dynamics of a dye-labeled DNA oligonucleotide or ‘‘aptamer’’ designed to bind specifically to immunoglobulin E. Rotational dynamics in combination with fluorescence lifetime measurements...

Wilson, George S.; Unruh, Jay R.; Gokulrangan, Giridharan; Lushington, Gerald H.; Johnson, Carey K.

2005-05-01T23:59:59.000Z

423

DNA ruler : enhancing nanopore sizing resolution by multiple measurements on the same DNA molecule  

E-Print Network [OSTI]

Nanopores are versatile sensors for label-free detection of single molecules and particles that have attracted attention for applications such as DNA sequencing and nanoparticle analysis. Detection of single molecules or ...

Sen, Yi-Heng

2012-01-01T23:59:59.000Z

424

DNA Damage-inducible Genes as Biomarkers for Exposures to Environmental Agents  

E-Print Network [OSTI]

A biodosimetric approach to determine alpha-particle dose to the respiratory tract epithelium from known exposures to radon has been developed in the rat. Cytotoxicity assays have been used to obtain dose-conversion factors for cumulative exposures typical of those encountered by underground uranium miners. However, this approach is not sensitive enough to derive doseconversion factors for indoor radon exposures. The expression of DNA damage-inducible genes is being investigated as a biomarker of exposure to radon progeny. Exposure of cultures of A549 cells to alpha particles resulted in an increase in the protein levels of the DNA damage-inducible genes, p53, Cip1, and Gadd45. These protein changes were associated with a transient arrest of cells passing through the cell cycle. This arrest was typified by an increase in the number of cells in the G, and G2 phases and a decrease in the number of cells in the S phase. The effect of inhaled alpha particles (radon progeny) in rats was examined in the epithelial cells of the lateral wall of the anterior nasal cavity. Exposures to radon progeny resulted in a significant increase in the number of cells in the G, phase and a decrease in the number of cells in the S phase. These cell-cycle changes were concomitant with an increase in the number of cells containing DNA strand breaks. These results suggest a commonality between cell-cycle events in vitro and in vivo following exposure to ionizing radiation. In addition to ionizing radiation, A549 cells were exposed to 4-nitroquinoline-1-oxide, methyl methanesulphonate, crocidolite asbestos, and glass microfiber. These studies showed that physical and chemical agents induce different expression patterns of p53, Cipl, and Gaddl 53 proteins and they could be used to discriminate between toxic and nontoxic materials such as asbestos and glass microfiber. The measurement of gene expression in A549 cells may provide a means to identify a broad spectrum of physical and chemical toxicants encountered in the environment. Environ Health Perspect 1 05(Suppl 4):913-918 (1997) Key words: radiation, fibers, chemicals, DNA damage-inducible genes

Neil F. Johnson; Thomas R. Carpenter; Richard J. Jaramillo; Teresa A. Liberati

425

A User's Guide to the Encyclopedia of DNA Elements (ENCODE)  

E-Print Network [OSTI]

The mission of the Encyclopedia of DNA Elements (ENCODE) Project is to enable the scientific and medical communities to

Bernstein, Bradley E.

426

Design and Characterization of Programmable DNA Nanotubes Supporting Information  

E-Print Network [OSTI]

. DNA sample preparation: Lyophilized HPLC- or PAGE- purified DNA oligonucleotides were purchased from Integrated DNA Technologies (Coralville, IA), resuspended in water, quanti- tated by UV absorbance at 260 nm immersion and 40X/0.75 NA air objectives. Blue light was filtered from a mer- cury arc lamp thro

Fygenson, Deborah Kuchnir

427

Self-assembling DNA templates for programmed artificial biomineralization  

E-Print Network [OSTI]

Self-assembling DNA templates for programmed artificial biomineralization Enrique C. Samano created via engineered DNA self-assembly represent an important new class of soft matter. These assemblies nanostructures Our current ability to create complex DNA nanostructures via designed self- assembly owes much

Finkelstein, Gleb

428

Correct and incorrect nucleotide incorporation pathways in DNA polymerase b  

E-Print Network [OSTI]

Correct and incorrect nucleotide incorporation pathways in DNA polymerase b Ravi Radhakrishnan a nucleotide incorporations in the DNA by using a novel protocol involving energy minimizations, dynamics simu- sive transient intermediates, for nucleotide incorporation at the template/primer DNA junction. A large

Schlick, Tamar

429

On the breaking of a plasma wave in a thermal plasma. I. The structure of the density singularity  

SciTech Connect (OSTI)

The structure of the singularity that is formed in a relativistically large amplitude plasma wave close to the wave breaking limit is found by using a simple waterbag electron distribution function. The electron density distribution in the breaking wave has a typical 'peakon' form. The maximum value of the electric field in a thermal breaking plasma is obtained and compared to the cold plasma limit. The results of computer simulations for different initial electron distribution functions are in agreement with the theoretical conclusions. The after-wavebreak regime is then examined, and a semi-analytical model of the density evolution is constructed. Finally the results of two dimensional particle in cell simulations for different initial electron distribution functions are compared, and the role of thermal effects in enhancing particle injection is noted.

Bulanov, Sergei V.; Esirkepov, Timur Zh.; Kando, Masaki; Koga, James K.; Pirozhkov, Alexander S.; Nakamura, Tatsufumi [QuBS, Japan Atomic Energy Agency, 1-8-7 Umemidai, Kizugawa, Kyoto 619-0215 (Japan); Bulanov, Stepan S. [University of California, Berkeley, California 94720 (United States); Schroeder, Carl B.; Esarey, Eric [Lawrence Berkeley National Laboratory, Berkeley, California 94720 (United States); Califano, Francesco; Pegoraro, Francesco [Physics Department, University of Pisa, Pisa 56127 (Italy)

2012-11-15T23:59:59.000Z

430

GeV BREAKS IN BLAZARS AS A RESULT OF GAMMA-RAY ABSORPTION WITHIN THE BROAD-LINE REGION  

SciTech Connect (OSTI)

Spectra of the brightest blazars detected by the Fermi Gamma-ray Space Telescope Large Area Telescope cannot be described by a simple power-law model. A much better description is obtained with a broken power law, with the break energies of a few GeV. We show here that the sharpness and the position of the breaks can be well reproduced by absorption of {gamma}-rays via photon-photon pair production on He II Lyman recombination continuum and lines. This implies that the blazar zone lies inside the region of the highest ionization of the broad-line region (BLR) within a light-year from a super-massive black hole. The observations of {gamma}-ray spectral breaks open a way of studying the BLR photon field in the extreme-UV/soft X-rays, which are otherwise hidden from our view.

Poutanen, Juri; Stern, Boris, E-mail: juri.poutanen@oulu.f, E-mail: boris.stern@gmail.co [Astronomy Division, Department of Physics, P.O. Box 3000, 90014 University of Oulu, Oulu (Finland)

2010-07-10T23:59:59.000Z

431

DNA digestion protocol & hints Overview: Although it is pretty standard to digest DNA with restriction enzymes, here  

E-Print Network [OSTI]

Liu 4/2004 DNA digestion protocol & hints Overview: Although it is pretty standard to digest DNA in molecular biology (3.1.1-3.1.2) Materials: · DNA sample in water or TE buffer · 10x digestion buffer.1 to 4 µg 10x Digestion buffer 2 µl 5 µl Enzyme ? ? Water Rest of volume Rest of volume 2. Add the enzyme

Doering, Tamara

432

Preparation of DNA-containing extract for PCR amplification  

DOE Patents [OSTI]

Environmental samples typically include impurities that interfere with PCR amplification and DNA quantitation. Samples of soil, river water, and aerosol were taken from the environment and added to an aqueous buffer (with or without detergent). Cells from the sample are lysed, releasing their DNA into the buffer. After removing insoluble cell components, the remaining soluble DNA-containing extract is treated with N-phenacylthiazolium bromide, which causes rapid precipitation of impurities. Centrifugation provides a supernatant that can be used or diluted for PCR amplification of DNA, or further purified. The method may provide a DNA-containing extract sufficiently pure for PCR amplification within 5–10 minutes.

Dunbar, John M.; Kuske, Cheryl R.

2006-07-11T23:59:59.000Z

433

The behavior of ANGRA 2 nuclear power plant core for a small break LOCA simulated with RELAP5 code  

SciTech Connect (OSTI)

This work discusses the behavior of Angra 2 nuclear power plant core, for a postulate Loss of Coolant Accident (LOCA) in the primary circuit for Small Break Loss Of Coolant Accident (SBLOCA). A pipe break of the hot leg Emergency Core Cooling System (ECCS) was simulated with RELAP 5 code. The considered rupture area is 380 cm{sup 2}, which represents 100% of the ECCS pipe flow area. Results showed that the cooling is enough to guarantee the integrity of the reactor core.

Sabundjian, Gaiane; Andrade, Delvonei A.; Belchior, Antonio Jr.; Silva Rocha, Marcelo da; Conti, Thadeu N.; Torres, Walmir M.; Macedo, Luiz A.; Umbehaun, Pedro E.; Mesquita, Roberto N.; Masotti, Paulo H. F.; Souza Lima, Ana Cecilia de [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN - SP) Av. Professor Lineu Prestes, 2242 05508-000 Sao Paulo, SP (Brazil)

2013-05-06T23:59:59.000Z

434

Edge of chaos as critical local symmetry breaking in dissipative nonautonomous systems  

E-Print Network [OSTI]

The fully nonlinear notion of resonance -$\\textit{geometrical resonance}$- in the general context of dissipative systems subjected to $\\textit{nonsteady}$ potentials is discussed. It is demonstrated that there is an exact local invariant associated with each geometrical resonance solution which reduces to the system's energy when the potential is steady. The geometrical resonance solutions represent a \\textit{local symmetry} whose critical breaking leads to a new analytical criterion for the order-chaos threshold. This physical criterion is deduced in the co-moving frame from the local energy conservation over the shortest significant timescale. Remarkably, the new criterion for the onset of chaos is shown to be valid over large regions of parameter space, thus being useful beyond the perturbative regime and the scope of current mathematical techniques.

Ricardo Chacón

2015-04-08T23:59:59.000Z

435

Symmetry breaking for ratchet transport in presence of interactions and magnetic field  

E-Print Network [OSTI]

We study the microwave induced ratchet transport of two-dimensional electrons on an oriented semidisk Galton board. The magnetic field symmetries of ratchet transport are analyzed in presence of electron-electron interactions. Our results show that a magnetic field asymmetric ratchet current can appear due to two contributions, a Hall drift of the rectified current that depends only weakly on electron-electron interactions and a breaking of the time reversal symmetry due to the combined effects of interactions and magnetic field. In the latter case, the asymmetry between positive and negative magnetic fields vanishes in the weak interaction limit. We also discuss the recent experimental results on ratchet transport in asymmetric nanostructures.

Leonardo Ermann; Alexei D. Chepelianskii; Dima L. Shepelyansky

2012-11-19T23:59:59.000Z

436

Critical Dynamics of Spontaneous Symmetry Breaking in a Homogeneous Bose gas  

E-Print Network [OSTI]

We explore the dynamics of spontaneous symmetry breaking in a homogeneous system by thermally quenching an atomic gas with short-range interactions through the Bose-Einstein phase transition. Using homodyne matter-wave interferometry to measure first-order correlation functions, we verify the central quantitative prediction of the Kibble-Zurek theory, namely the homogeneous-system power-law scaling of the coherence length with the quench rate. Moreover, we directly confirm its underlying hypothesis, the freezing of the correlation length near the transition due to critical slowing down. Our measurements agree with beyond mean-field theory, and support the previously unverified expectation that the dynamical critical exponent for this universality class, which includes the $\\lambda$-transition of liquid $^4$He, is $z=3/2$.

Nir Navon; Alexander L. Gaunt; Robert P. Smith; Zoran Hadzibabic

2014-10-30T23:59:59.000Z

437

General relativity as the effective theory of GL(4,R) spontaneous symmetry breaking  

SciTech Connect (OSTI)

We assume a GL(4,R) space-time symmetry which is spontaneously broken to SO(3,1). We carry out the coset construction of the effective theory for the nonlinearly realized broken symmetry in terms of the Goldstone fields and matter fields transforming linearly under the unbroken Lorentz subgroup. We then identify functions of the Goldstone and matter fields that transform linearly also under the broken symmetry. Expressed in terms of these quantities the effective theory reproduces the vierbein formalism of general relativity with general coordinate invariance being automatically realized nonlinearly over GL(4,R). The coset construction makes no assumptions about any underlying theory that might be responsible for the assumed symmetry breaking. We give a brief discussion of the possibility of field theories with GL(4,R) rather than Lorentz space-time symmetry providing the underlying dynamics.

Tomboulis, E. T. [Department of Physics and Astronomy, UCLA, Los Angeles, California 90095-1547 (United States)

2011-10-15T23:59:59.000Z

438

General Relativity as the effective theory of GL(4,R) spontaneous symmetry breaking  

E-Print Network [OSTI]

We assume a GL(4,R) space-time symmetry which is spontaneously broken to SO(3,1). We carry out the coset construction of the effective theory for the non-linearly realized broken symmetry in terms of the Goldstone fields and matter fields transforming linearly under the unbroken Lorentz subgroup. We then identify functions of the Goldstone and matter fields that transform linearly also under the broken symmetry. Expressed in terms of these quantities the effective theory reproduces the vierbein formalism of General Relativity with general coordinate invariance being automatically realized non-linearly over GL(4,R). The coset construction makes no assumptions about any underlying theory that might be responsible for the assumed symmetry breaking. We give a brief discussion of the possibility of field theories with GL(4,R) rather than Lorentz space-time symmetry providing the underlying dynamics.

E. T. Tomboulis

2011-05-30T23:59:59.000Z

439

Chiral-Symmetry Breaking in Pseudo Quantum Electrodynamics at Finite Temperature  

E-Print Network [OSTI]

We use the Schwinger-Dyson equations in the presence of a thermal bath, in order to study chiral symmetry breaking in a system of massless Dirac fermions interacting through pseudo quantum electrodynamics (PQED3), in (2+1) dimensions. We show that there is a critical temperature $T_c$, below which chiral symmetry is broken, and a corresponding mass gap is dynamically generated, provided the coupling is above a certain, temperature dependent, critical value $\\alpha_c$. The ratio between the energy gap and the critical temperature for this model is estimated to be $2 \\pi$. These results are confirmed by analytical and numerical investigations of the Schwinger-Dyson equation for the electron. In addition, we calculate the first finite-temperature corrections to the static Coulomb interaction. The relevance of this result in the realm of condensed matter systems, like graphene, is briefly discussed.

Leandro O. Nascimento; Van Sérgio Alves; Francisco Peña; C. Morais Smith; E. C. Marino

2015-03-28T23:59:59.000Z

440

Evidence of BRST-Symmetry Breaking in Lattice Minimal Landau Gauge  

E-Print Network [OSTI]

By evaluating the so-called Bose-ghost propagator, we present the first numerical evidence of BRST-symmetry breaking for Yang-Mills theory in minimal Landau gauge, i.e. due to the restriction of the functional integration to the first Gribov region in the Gribov-Zwanziger approach. Our data are well described by a simple fitting function, which can be related to a massive gluon propagator in combination with an infrared-free (Faddeev-Popov) ghost propagator. As a consequence, the Bose-ghost propagator, which has been proposed as a carrier of the confining force in minimal Landau gauge, displays a 1/p^4 singularity in the infrared limit.

Attilio Cucchieri; David Dudal; Tereza Mendes; Nele Vandersickel

2014-10-30T23:59:59.000Z

Note: This page contains sample records for the topic "dna double-strand breaks" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


441

Practical applications of the R6 leak-before-break procedure  

SciTech Connect (OSTI)

A forthcoming revision to the R6 Leak-before-Break Assessment Procedure is briefly described. Practical application of the LbB concepts to safety-critical nuclear plant is illustrated by examples covering both low temperature and high temperature (>450{degrees}C) operating regimes. The examples highlight a number of issues which can make the development of a satisfactory LbB case problematic: for example, coping with highly loaded components, methodology assumptions and the definition of margins, the effect of crack closure owing to weld residual stresses, complex thermal stress fields or primary bending fields, the treatment of locally high stresses at crack intersections with free surfaces, the choice of local limit load solution when predicting ligament breakthrough, and the scope of calculations required to support even a simplified LbB case for high temperature steam pipe-work systems.

Bouchard, P.J.

1997-04-01T23:59:59.000Z

442

Specialist meeting on leak before break in reactor piping and vessels  

SciTech Connect (OSTI)

A series of research projects sponsored by the Federal Minister for Education, Science, Research and Technology, Bonn are summarized and compared to utility, manufacturer, and vendor tests. The purpose of the evaluation was to experimentally verify Leak-before-Break behavior, confirm the postulation of fracture preclusion for piping (straight pipe, bends and branches), and quantify the safety margin against massive failure. The results are applicable to safety assessment of ferritic and austenitic piping in primary and secondary nuclear power plant circuits. Moreover, because of the wide range of the test parameters, they are also important for the design and assessment of piping in other technical plant. The test results provide justification for ruling out catastrophic fractures, even on pipes of dimensions corresponding to those of a main coolant pipe of a pressurized water reactor plant on the basis of a mechanical deterministic safety analysis in correspondence with the Basis Safety Concept (Principle of Fracture Exclusion).

Bartholome, G.; Bazant, E.; Wellein, R. [Siemens KWU, Stuttgart (Germany)] [and others

1997-04-01T23:59:59.000Z

443

Spontaneous breaking of symmetry of the gravitons of the long wave spectrum in the early Universe  

E-Print Network [OSTI]

It is shown that nonlinear terms in equations of gravitons on the background of curved space-time of the expanding Universe can solve the problem of the negative square of the effective mass formally arising in linear approximation for gravitons. Similar to well known spontaneous breaking of symmetry in Goldstone model one must take another vacuum so that nonzero vacuum expectation value of the quantized graviton field leads to change of spectrum for gravitons. There appears two graviton fields, one with the positive mass, another with the zero mass. Energy density and the density of particles created by gravitation of the expanding Universe are calculated for some special cases of the scale factor. Numerical of result are obtained for the dust universe case.

A. A. Grib

2009-05-07T23:59:59.000Z

444

Order statistics inference for describing topological coupling and mechanical symmetry breaking in multidomain proteins  

E-Print Network [OSTI]

Cooperativity is a hallmark of proteins, many of which show a modular architecture comprising discrete structural domains. Detecting and describing dynamic couplings between structural regions is difficult in view of the many-body nature of protein-protein interactions. By utilizing the GPU-based computational acceleration, we carried out simulations of the protein forced unfolding for the dimer WW-WW of the all-beta-sheet WW domains used as a model multidomain protein. We found that while the physically non-interacting identical protein domains (WW) show nearly symmetric mechanical properties at low tension, reflected, e.g., in the similarity of their distributions of unfolding times, these properties become distinctly different when tension is increased. Moreover, the uncorrelated unfolding transitions at a low pulling force become increasingly more correlated (dependent) at higher forces. Hence, the applied force not only breaks "the mechanical symmetry" but also couples the physically non-interacting prot...

Kononova, Olga; Barsegov, Valeri

2015-01-01T23:59:59.000Z

445

Autonomous Motility of Active Filaments due to Spontaneous Flow-Symmetry Breaking  

E-Print Network [OSTI]

We simulate the nonlocal Stokesian hydrodynamics of an elastic filament which is active due a permanent distribution of stresslets along its contour. A bending instability of an initially straight filament spontaneously breaks flow symmetry and leads to autonomous filament motion which, depending on conformational symmetry, can be translational or rotational. At high ratios of activity to elasticity, the linear instability develops into nonlinear fluctuating states with large amplitude deformations. The dynamics of these states can be qualitatively understood as a superposition of translational and rotational motion associated with filament conformational modes of opposite symmetry. Our results can be tested in molecular-motor filament mixtures, synthetic chains of autocatalytic particles, or other linearly connected systems where chemical energy is converted to mechanical energy in a fluid environment.

Gayathri Jayaraman; Sanoop Ramachandran; Somdeb Ghose; Abhrajit Laskar; M. Saad Bhamla; P. B. Sunil Kumar; R. Adhikari

2012-10-10T23:59:59.000Z

446

Spatio-temporal evolution and breaking of double layers: A description using Lagrangian hydrodynamics  

SciTech Connect (OSTI)

The nonlinear development and collapse (breaking) of double layers in the long scale length limit is well described by equations for the cold ion fluid with quasineutrality. It is shown that electron dynamics is responsible for giving an 'equation of state' with negative ratio of specific heats to this fluid. Introducing a transformation for the density variable, the governing equation for the transformed quantity in terms of Lagrange variables turns out exactly to be a linear partial differential equation. This equation has been analyzed in various limits of interest. Nonlinear development of double layers with a sinusoidal initial disturbance and collapse of double layers with an initial perturbation in the form of a density void are analytically investigated.

Kaw, Predhiman; Sengupta, Sudip; Singh Verma, Prabal [Institute for Plasma Research, Bhat, Gandhinagar 382 428 (India)

2012-10-15T23:59:59.000Z

447

Distinct kinetics of human DNA ligases I, IIIalpha, IIIbeta, and IV reveal direct DNA sensing ability and differential physiological functions in DNA repair  

SciTech Connect (OSTI)

The three human LIG genes encode polypeptides that catalyze phosphodiester bond formation during DNA replication, recombination and repair. While numerous studies have identified protein partners of the human DNA ligases (hLigs), there has been little characterization of the catalytic properties of these enzymes. In this study, we developed and optimized a fluorescence-based DNA ligation assay to characterize the activities of purified hLigs. Although hLigI joins DNA nicks, it has no detectable activity on linear duplex DNA substrates with short, cohesive single-strand ends. By contrast, hLigIII{beta} and the hLigIII{alpha}/XRCC1 and hLigIV/XRCC4 complexes are active on both nicked and linear duplex DNA substrates. Surprisingly, hLigIV/XRCC4, which is a key component of the major non-homologous end joining (NHEJ) pathway, is significantly less active than hLigIII on a linear duplex DNA substrate. Notably, hLigIV/XRCC4 molecules only catalyze a single ligation event in the absence or presence of ATP. The failure to catalyze subsequent ligation events reflects a defect in the enzyme-adenylation step of the next ligation reaction and suggests that, unless there is an in vivo mechanism to reactivate DNA ligase IV/XRCC4 following phosphodiester bond formation, the cellular NHEJ capacity will be determined by the number of adenylated DNA ligaseIV/XRCC4 molecules.

Chen, Xi; Ballin, Jeff D.; Della-Maria, Julie; Tsai, Miaw-Sheue; White, Elizabeth J.; Tomkinson, Alan E.; Wilson, Gerald M.

2009-05-11T23:59:59.000Z

448

Determining orientation and direction of DNA sequences  

DOE Patents [OSTI]

Determining orientation and direction of DNA sequences. A method by which fluorescence in situ hybridization can be made strand specific is described. Cell cultures are grown in a medium containing a halogenated nucleotide. The analog is partially incorporated in one DNA strand of each chromatid. This substitution takes place in opposite strands of the two sister chromatids. After staining with the fluorescent DNA-binding dye Hoechst 33258, cells are exposed to long-wavelength ultraviolet light which results in numerous strand nicks. These nicks enable the substituted strand to be denatured and solubilized by heat, treatment with high or low pH aqueous solutions, or by immersing the strands in 2.times.SSC (0.3M NaCl+0.03M sodium citrate), to name three procedures. It is unnecessary to enzymatically digest the strands using Exo III or another exonuclease in order to excise and solubilize nucleotides starting at the sites of the nicks. The denaturing/solubilizing process removes most of the substituted strand while leaving the prereplication strand largely intact. Hybridization of a single-stranded probe of a tandem repeat arranged in a head-to-tail orientation will result in hybridization only to the chromatid with the complementary strand present.

Goodwin, Edwin H. (Los Alamos, NM); Meyne, Julianne (Los Alamos, NM)

2000-01-01T23:59:59.000Z

449

A Mechanism-Based Approach to Predict the Relative Biological Effectiveness of Protons and Carbon Ions in Radiation Therapy  

SciTech Connect (OSTI)

Purpose: The physical and potential biological advantages of proton and carbon ions have not been fully exploited in radiation therapy for the treatment of cancer. In this work, an approach to predict proton and carbon ion relative biological effectiveness (RBE) in a representative spread-out Bragg peak (SOBP) is derived using the repair-misrepair-fixation (RMF) model. Methods and Materials: Formulas linking dose-averaged linear-quadratic parameters to DSB induction and processing are derived from the RMF model. The Monte Carlo Damage Simulation (MCDS) software is used to quantify the effects of radiation quality on the induction of DNA double-strand breaks (DSB). Trends in parameters {alpha} and {beta} for clinically relevant proton and carbon ion kinetic energies are determined. Results: Proton and carbon ion RBE are shown to increase as particle energy, dose, and tissue {alpha}/{beta} ratios decrease. Entrance RBE is {approx}1.0 and {approx}1.3 for protons and carbon ions, respectively. For doses in the range of 0.5 to 10 Gy, proton RBE ranges from 1.02 (proximal edge) to 1.4 (distal edge). Over the same dose range, the RBE for carbon ions ranges from 1.5 on the proximal edge to 6.7 on the distal edge. Conclusions: The proposed approach is advantageous because the RBE for clinically relevant particle distributions is guided by well-established physical and biological (track structure) considerations. The use of an independently tested Monte Carlo model to predict the effects of radiation quality on DSB induction also minimizes the number of ad hoc biological parameters that must be determined to predict RBE. Large variations in predicted RBE across an SOBP may produce undesirable biological hot and cold spots. These results highlight the potential for the optimization of physical dose for a uniform biological effect.

Frese, Malte C. [Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, Connecticut (United States); Department of Medical Physics in Radiation Oncology, German Cancer Research Center, Heidelberg (Germany); Yu, Victor K. [Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, Connecticut (United States); Stewart, Robert D. [Department of Radiation Oncology, University of Washington, Seattle, Washington (United States); Carlson, David J., E-mail: david.j.carlson@yale.edu [Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, Connecticut (United States)

2012-05-01T23:59:59.000Z

450

Dual effects of N-acetyl-L-cysteine dependent on NQO1 activity: Suppressive or promotive of 9,10-phenanthrenequinone-induced toxicity  

SciTech Connect (OSTI)

A typical antioxidant, N-acetyl-L-cysteine (NAC) generally protects cells from oxidative damage induced by reactive oxygen species (ROS). 9,10-Phenanthrenequinone (9,10-PQ), a major quinone in diesel exhaust particles, produces ROS in redox cycling following two-electron reduction by NAD(P)H:quinone oxidoreductase 1 (NQO1), which has been considered as a cause of its cyto- and genotoxicity. In this study, we show that NAC unexpectedly augments the toxicity of 9,10-PQ in cells with low NQO1 activity. In four human skin cell lines, the expression and the activity of NQO1 were lower than in human adenocarcinoma cell lines, A549 and MCF7. In the skin cells, the cytotoxicity of 9,10-PQ was significantly enhanced by addition of NAC. The formation of DNA double strand breaks accompanying phosphorylation of histone H2AX, was also remarkably augmented. On the other hand, the cyto- and genotoxicity were suppressed by addition of NAC in the adenocarcinoma cells. Two contrasting experiments: overexpression of NQO1 in CHO-K1 cells which originally expressed low NQO1 levels, and knock?down of NQO1 in the adenocarcinoma cell line A549 by transfection of RNAi, also showed that NAC suppressed 9,10-PQ-induced toxicity in cell lines expressing high NQO1 activity and enhanced it in cell lines with low NQO1 activity. The results suggested that dual effects of NAC on the cyto- and genotoxicity of 9,10-PQ were dependent on tissue-specific NQO1 activity. -- Highlights: ? NAC augmented the cytotoxicity of 9,10-PQ in skin cell lines. ? 9,10-PQ-induced DSBs accompanying ?-H2AX were also augmented by NAC. ? NAC suppressed the cyto- and genotoxicity of 9,10-PQ in adenocarcinoma cell lines. ? The dual effects of NAC on toxicity of 9,10-PQ were dependent on NQO1 activity.

Toyooka, Tatsushi; Shinmen, Takuya [Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, Shizuoka (Japan)] [Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, Shizuoka (Japan); Aarts, Jac M.M.J.G. [Division of Toxicology, Wageningen University, Wageningen (Netherlands); Ibuki, Yuko, E-mail: ibuki@u-shizuoka-ken.ac.jp [Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, Shizuoka (Japan)] [Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, Shizuoka (Japan)

2012-11-01T23:59:59.000Z

451

MLN8054, A Small Molecule Inhibitor of Aurora Kinase A, Sensitizes Androgen-Resistant Prostate Cancer to Radiation;Aurora kinase A; MLN8054; Prostate cancer; Radiation  

SciTech Connect (OSTI)

Purpose: To determine whether MLN8054, an Aurora kinase A (Aurora-A) inhibitor causes radiosensitization in androgen-insensitive prostate cancer cells in vitro and in vivo. Methods and Materials: In vitro studies consisted of culturing PC3 and DU145 prostate cancer cells and then immunoblotting Aurora A and phospho-Aurora A after radiation and/or nocodazole with MLN8054. Phases of the cell cycle were measured with flow cytometry. PC3 and DU145 cell lines were measured for survival after treatment with MLN8054 and radiation. Immunofluorescence measured {gamma}-H2AX in the PC3 and DU145 cells after treatment. In vivo studies looked at growth delay of PC3 tumor cells in athymic nude mice. PC3 cells grew for 6 to 8 days in mice treated with radiation, MLN8054, or combined for 7 more days. Tumors were resected and fixed on paraffin and stained for von Willebrand factor, Ki67, and caspase-3. Results: In vitro inhibition of Aurora-A by MLN8054 sensitized prostate cancer cells, as determined by dose enhancement ratios in clonogenic assays. These effects were associated with sustained DNA double-strand breaks, as evidenced by increased immunofluorescence for {gamma}-H2AX and significant G2/M accumulation and polyploidy. In vivo, the addition of MLN8054 (30 mg/kg/day) to radiation in mouse prostate cancer xenografts (PC3 cells) significantly increased tumor growth delay and apoptosis (caspase-3 staining), with reduction in cell proliferation (Ki67 staining) and vascular density (von Willebrand factor staining). Conclusion: MLN8054, a novel small molecule Aurora-A inhibitor showed radiation sensitization in androgen-insensitive prostate cancer in vitro and in vivo. This warrants the clinical development of MLN8054 with radiation for prostate cancer patients.

Moretti, Luigi [Department of Radiation Oncology, Vanderbilt-Ingram Cancer Center, Vanderbilt University, Nashville, Tennessee (United States); Department of Radiation Oncology, Institut Jules Bordet, Universite Libre de Bruxelles, Brussels (Belgium); Niermann, Kenneth; Schleicher, Stephen; Giacalone, Nicholas J.; Varki, Vinod; Kim, Kwang Woon; Kopsombut, Prapaporn; Jung, Dae Kwang [Department of Radiation Oncology, Vanderbilt-Ingram Cancer Center, Vanderbilt University, Nashville, Tennessee (United States); Bo Lu, E-mail: bo.lu@vanderbilt.edu [Department of Radiation Oncology, Vanderbilt-Ingram Cancer Center, Vanderbilt University, Nashville, Tennessee (United States)

2011-07-15T23:59:59.000Z

452

Warm wave breaking of nonlinear plasma waves with arbitrary phase velocities C. B. Schroeder, E. Esarey, and B. A. Shadwick  

E-Print Network [OSTI]

Warm wave breaking of nonlinear plasma waves with arbitrary phase velocities C. B. Schroeder, E, collisionless plasma is developed to analyze nonlinear plasma waves excited by intense drive beams. The maximum amplitude and wavelength are calculated for nonrelativistic plasma temperatures and arbitrary plasma wave

Geddes, Cameron Guy Robinson

453

New LM Unit Root Tests in the Presence of a Possible Break of Unknown Date and Size  

E-Print Network [OSTI]

New LM Unit Root Tests in the Presence of a Possible Break of Unknown Date and Size John T. Cuddington and Shuichiro Nishioka December 6, 2005 Abstract: Lagrange Multiplier (LM) unit root tests performs poorly when used in LM unit root tests and propose an alternative procedure that uses the sup

454

TabletopAccelerator Breaks`Cold Fusion'Jinx ButWon'tYield Energy,Physicists Say  

E-Print Network [OSTI]

TabletopAccelerator Breaks`Cold Fusion'Jinx ButWon'tYield Energy,Physicists Say A crystal with a strange property is at the heart of a clever method for inducing nuclear fusion in a tabletop-sized device-rays for medical therapies. Although the field of room-temperature fusion is littered with scandals and dubious

455

LED Light Fixture Project FC1 Director's Conference Room: Life Cycle Cost and Break-even Analysis  

E-Print Network [OSTI]

. A light-emitting diode (LED) is a solid-state lighting source that switches on instantly, is readilyLED Light Fixture Project ­ FC1 Director's Conference Room: Life Cycle Cost and Break-even Analysis light fixtures in existing or new buildings across campus. Scope of Work On August 27, 2012, the six

Johnston, Daniel

456

Forecasting the conditional volatility of oil spot and futures prices with structural breaks and long memory models  

E-Print Network [OSTI]

Forecasting the conditional volatility of oil spot and futures prices with structural breaks of oil spot and futures prices using three GARCH-type models, i.e., linear GARCH, GARCH with structural that oil price fluctuations influence economic activity and financial sector (e.g., Jones and Kaul, 1996

Paris-Sud XI, Université de

457

Active methane venting observed at giant pockmarks along the U.S. mid-Atlantic shelf break  

E-Print Network [OSTI]

Active methane venting observed at giant pockmarks along the U.S. mid-Atlantic shelf break Kori R the edge of the mid-Atlantic continental shelf confirms that methane is actively venting at the site. Dissolved methane concentrations, which were measured with a commercially available methane sensor (METS

Eustice, Ryan

458

JET BREAKS IN SHORT GAMMA-RAY BURSTS. I. THE UNCOLLIMATED AFTERGLOW OF GRB 050724 Dirk Grupe,1  

E-Print Network [OSTI]

JET BREAKS IN SHORT GAMMA-RAY BURSTS. I. THE UNCOLLIMATED AFTERGLOW OF GRB 050724 Dirk Grupe,1 the results of the Chandra observations of the Swift-discovered short gamma-ray burst GRB 050724. Chandra corrected energy of GRB 050724 is at least 4 ; 1049 ergs. Subject headinggs: gamma rays: bursts -- X-rays

Zhang, Bing

459

Hydrogen Bond Breaking and Reformation in Alcohol Oligomers Following Vibrational Relaxation of a Non-Hydrogen-Bond Donating Hydroxyl Stretch  

E-Print Network [OSTI]

Hydrogen Bond Breaking and Reformation in Alcohol Oligomers Following Vibrational Relaxation of a Non-Hydrogen-Bond Donating Hydroxyl Stretch K. J. Gaffney, I. R. Piletic, and M. D. Fayer* Department measured with ultrafast infrared pump-probe experiments. Non-hydrogen-bond donating OD stretches (2690 cm-1

Fayer, Michael D.

460

Probing Minor Groove Hydrogen Bonding Interactions between RB69 DNA Polymerase and DNA  

SciTech Connect (OSTI)

Minor groove hydrogen bonding (HB) interactions between DNA polymerases (pols) and N3 of purines or O2 of pyrimidines have been proposed to be essential for DNA synthesis from results obtained using various nucleoside analogues lacking the N3 or O2 contacts that interfered with primer extension. Because there has been no direct structural evidence to support this proposal, we decided to evaluate the contribution of minor groove HB interactions with family B pols. We have used RB69 DNA pol and 3-deaza-2'-deoxyadenosine (3DA), an analogue of 2-deoxyadenosine, which has the same HB pattern opposite T but with N3 replaced with a carbon atom. We then determined pre-steady-state kinetic parameters for the insertion of dAMP opposite dT using primer/templates (P/T)-containing 3DA. We also determined three structures of ternary complexes with 3DA at various positions in the duplex DNA substrate. We found that the incorporation efficiency of dAMP opposite dT decreased 10{sup 2}-10{sup 3}-fold even when only one minor groove HB interaction was missing. Our structures show that the HB pattern and base pair geometry of 3DA/dT is exactly the same as those of dA/dT, which makes 3DA an optimal analogue for probing minor groove HB interactions between a DNA polymerase and a nucleobase. In addition, our structures provide a rationale for the observed 10{sup 2}-10{sup 3}-fold decrease in the rate of nucleotide incorporation. The minor groove HB interactions between position n-2 of the primer strand and RB69pol fix the rotomer conformations of the K706 and D621 side chains, as well as the position of metal ion A and its coordinating ligands, so that they are in the optinal orientation for DNA synthesis.

Xia, Shuangluo; Christian, Thomas D.; Wang, Jimin; Konigsberg, William H. (Yale)

2012-09-17T23:59:59.000Z

Note: This page contains sample records for the topic "dna double-strand breaks" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


461

2/1/2014 Financial Press -Breaking Business & Economic News http://financialpress.com/2014/01/21/teeny-tiny-windmills-could-recharge-phones/ 1/6  

E-Print Network [OSTI]

2/1/2014 Financial Press - Breaking Business & Economic News http://financialpress.com/2014 Twitter Facebook Google #12;2/1/2014 Financial Press - Breaking Business & Economic News http fit on a single grain of rice), can harvest energy from the movement of air. They are the work

Chiao, Jung-Chih

462

Double Beta Decay, Lepton Flavour Violation and Collider Signatures of Left-Right Symmetric Models with Spontaneous D Parity Breaking  

E-Print Network [OSTI]

We propose a class of left-right symmetric models (LRSMs) with spontaneous D parity breaking, where SU(2)_R breaks at the TeV scale while discrete left-right symmetry breaks around 10^9 GeV. By embedding this framework in a non-supersymmetric SO(10) Grand Unified Theory (GUT) with Pati-Salam symmetry as the highest intermediate breaking step, we obtain g_R / g_L ~ 0.6 between the right- and left-handed gauge couplings at the TeV scale. This leads to a suppression of beyond the Standard Model phenomena induced by the right-handed gauge coupling. Here we focus specifically on the consequences for neutrinoless double beta decay, low energy lepton flavour violation and LHC signatures due to the suppressed right handed currents. Interestingly, the reduced g_R allows us to interpret an excess of events observed recently in the range of 1.9 TeV to 2.4 TeV by the CMS group at the LHC as the signature of a right handed gauge boson in LRSMs with spontaneous D parity breaking. Moreover, the reduced right-handed gauge coupling also strongly suppresses the non-standard contribution of heavy states to the neutrinoless double beta decay rate as well as the amplitude of low energy lepton flavour violating processes. In a dominant type-II Seesaw mechanism of neutrino mass generation, we find that both sets of observables provide stringent and complimentary bounds which make it challenging to observe the scenario at the LHC.

Frank F. Deppisch; Tomas E. Gonzalo; Sudhanwa Patra; Narendra Sahu; Utpal Sarkar

2014-10-23T23:59:59.000Z

463

DNA damage-inducible genes as biomarkers for exposures to environmental agents  

SciTech Connect (OSTI)

A biodosimetric approach to determine alpha-particle dose to the respiratory tract epithelium from known exposures to radon has been developed in the rat. Cytotoxicity assays have been used to obtain dose-conversion factors for cumulative exposures typical of those encountered by underground uranium miners. However, this approach is not sensitive enough to derive close-conversion factors for indoor radon exposures. The expression of DNA damage-inducible genes is being investigated as a biomarker of exposure to radon progeny. Exposure of cultures of A549 cells to alpha particles resulted in an increase in the protein levels of the DNA damage-inducible genes, p53, Cip 1, and Gadd45. These protein changes were associated with a transient arrest of cells passing through the cell cycle. This arrest was typified by an increase in the number of cells in the G{sub 1} and G{sub 2} phases and a decrease in the number of cells in the S phase. The effect of inhaled alpha particles (radon progeny) in rats was examined in the epithelial cells of the lateral wall of the anterior nasal cavity. Exposures to radon progeny resulted in a significant increase in the number of cells in the G{sub 1} phase and a decrease in the number of cells in the S phase. These cell-cycle changes were concomitant with an increase in the number of cells containing DNA strand breaks. In addition to ionizing radiation, A549 cells were exposed to 4-nitroquinoline-1-oxide, methyl methanesulphonate, crocidolite asbestos, and glass microfiber. These studies showed that physical and chemical agents induce different expression patterns of p53, Cip 1, and Gadd153 proteins and they could be used to discriminate between toxic and nontoxic materials such as asbestos and glass microfiber. The measurement of gene expression in A549 cells may provide a means to identify a broad spectrum of physical and chemical toxicants encountered in the environment. 9 figs., 42 refs.

Johnson, N.F.; Carpenter, T.R.; Jaramillo, R.J.; Liberati, T.A. [Inhalation Toxicology Research Institute, Albuquerque, NM (United States)

1997-06-01T23:59:59.000Z

464

Osmotic pressure: resisting or promoting DNA ejection from phage  

E-Print Network [OSTI]

Recent in vitro experiments have shown that DNA ejection from bacteriophage can be partially stopped by surrounding osmotic pressure when ejected DNA is digested by DNase I on the course of ejection. We argue in this work by combination of experimental techniques (osmotic suppression without DNaseI monitored by UV absorbance, pulse-field electrophoresis, and cryo-EM visualization) and simple scaling modeling that intact genome (i.e. undigested) ejection in a crowded environment is, on the contrary, enhanced or eventually complete with the help of a pulling force resulting from DNA condensation induced by the osmotic stress itself. This demonstrates that in vivo, the osmotically stressed cell cytoplasm will promote phage DNA ejection rather than resisting it. The further addition of DNA-binding proteins under crowding conditions is shown to enhance the extent of ejection. We also found some optimal crowding conditions for which DNA content remaining in the capsid upon ejection is maximum, which correlates well...

Jeembaeva, Meerim; Larsson, Frida; Evilevitch, Alex

2008-01-01T23:59:59.000Z

465

Capstan friction model for DNA ejection from bacteriophages  

E-Print Network [OSTI]

Bacteriophages infect cells by attaching to the outer membrane and injecting their DNA into the cell.The phage DNA is then transcribed by the cell's transcription machinery.A number of physical mechanisms by which DNA can be translocated from the phage capsid into the cell have been identified. A fast ejection driven by the elastic and electrostatic potential energy of the compacted DNA within the viral capsid appears to be used by most phages, at least to initiate infection.In recent in vitro experiments, the speed of DNA translocation from a lambda phage capsid has been measured as a function of ejected length over the entire duration of the event.Here a mechanical model is proposed that is able to explain the observed dependence of exit velocity on ejected length, and that is also consistent with the accepted picture of the geometric arrangement of DNA within the viral capsid.

Sandip Ghosal

2013-01-09T23:59:59.000Z

466

How effective is graphene nanopore geometry on DNA sequencing?  

E-Print Network [OSTI]

In this paper we investigate the effects of graphene nanopore geometry on homopolymer ssDNA pulling process through nanopore using steered molecular dynamic (SMD) simulations. Different graphene nanopores are examined including axially symmetric and asymmetric monolayer graphene nanopores as well as five layer graphene polyhedral crystals (GPC). The pulling force profile, moving fashion of ssDNA, work done in irreversible DNA pulling and orientations of DNA bases near the nanopore are assessed. Simulation results demonstrate the strong effect of the pore shape as well as geometrical symmetry on free energy barrier, orientations and dynamic of DNA translocation through graphene nanopore. Our study proposes that the symmetric circular geometry of monolayer graphene nanopore with high pulling velocity can be used for DNA sequencing.

Satarifard, Vahid; Ejtehadi, Mohammad Reza

2015-01-01T23:59:59.000Z

467

A lead-before-break strategy for primary heat transport piping of 500 MWe Indian PHWR  

SciTech Connect (OSTI)

Leak-Before-Break (LBB) is being used to design the primary heat transport piping system of 500 MWe Indian Pressurized Heavy Water Reactors (IPHWR). The work is categorized in three directions to demonstrate three levels of safety against sudden catastrophic break. Level 1 is inherent in the design procedure of piping system as per ASME Sec.III with a well defined factor of safety. Level 2 consists of fatigue crack growth study of a postulated part-through flaw at the inside surface of pipes. Level 3 is stability analysis of a postulated leakage size flaw under the maximum credible loading condition. Developmental work related to demonstration of level 2 and level 3 confidence is described in this paper. In a case study on fatigue crack growth on PHT straight pipes for level 2, negligible crack growth is predicted for the life of the reactor. For level 3 analysis, the R6 method has been adopted. A database to evaluate SIF of elbows with throughwall flaws under combined internal pressure and bending moment has been generated to provide one of the inputs for R6 method. The methodology of safety assessment of elbow using R6 method has been demonstrated for a typical pump discharge elbow. In this analysis, limit load of the cracked elbow has been determined by carrying out elasto-plastic finite element analysis. The limit load results compared well with those given by Miller. However, it requires further study to give a general form of limit load solution. On the experimental front, a set of small diameter pipe fracture experiments have been carried out at room temperature and 300{degrees}C. Two important observations of the experiments are - appreciable drop in maximum load at 300{degrees}C in case of SS pipes and out-of-plane crack growth in case of CS pipes. Experimental load deflection curves are finally compared with five J-estimation schemes predictions. A material database of PHT piping materials is also being generated for use in LBB analysis.

Chattopadhyay, J.; Dutta, B.K.; Kushwaha, H.S. [Bhabha Atomic Research Centre, Bombay (India)] [and others

1997-04-01T23:59:59.000Z

468

EVIDENCE FOR ELEVATED X-RAY EMISSION IN LOCAL LYMAN BREAK GALAXY ANALOGS  

SciTech Connect (OSTI)

Our knowledge of how X-ray emission scales with star formation at the earliest times in the universe relies on studies of very distant Lyman break galaxies (LBGs). In this paper, we study the relationship between the 2-10 keV X-ray luminosity (L{sub X}), assumed to originate from X-ray binaries (XRBs), and star formation rate (SFR) in ultraviolet (UV) selected z < 0.1 Lyman break analogs (LBAs). We present Chandra observations for four new Galaxy Evolution Explorer selected LBAs. Including previously studied LBAs, Haro 11 and VV 114, we find that LBAs demonstrate L{sub X}/SFR ratios that are elevated by {approx}1.5{sigma} compared to local galaxies, similar to the ratios found for stacked LBGs in the early universe (z > 2). Unlike some of the composite LBAs studied previously, we show that these LBAs are unlikely to harbor active galactic nuclei, based on their optical and X-ray spectra and the spatial distribution of the X-rays in three spatially extended cases. Instead, we expect that high-mass X-ray binaries (HMXBs) dominate the X-ray emission in these galaxies, based on their high specific SFRs (sSFRs {identical_to} SFR/M{sub *} {>=} 10{sup -9} yr{sup -1}), which suggest the prevalence of young stellar populations. Since both UV-selected populations (LBGs and LBAs) have lower dust attenuations and metallicities compared to similar samples of more typical local galaxies, we investigate the effects of dust extinction and metallicity on the L{sub X}/SFR for the broader population of galaxies with high sSFRs (>10{sup -10} yr{sup -1}). The estimated dust extinctions (corresponding to column densities of N{sub H} < 10{sup 22} cm{sup -2}) are expected to have insignificant effects on observed L{sub X}/SFR ratio for the majority of galaxy samples. We find that the observed relationship between L{sub X}/SFR and metallicity appears consistent with theoretical expectations from XRB population synthesis models. Therefore, we conclude that lower metallicities, related to more luminous HMXBs such as ultraluminous X-ray sources, drive the elevated L{sub X}/SFR observed in our sample of z < 0.1 LBAs. The relatively metal-poor, active mode of star formation in LBAs and distant z > 2 LBGs may yield higher total HMXB luminosity than found in typical galaxies in the local universe.

Basu-Zych, Antara R.; Lehmer, Bret D.; Hornschemeier, Ann E.; Ptak, Andrew F. [NASA Goddard Space Flight Center, Code 662, Greenbelt, MD 20771 (United States); Goncalves, Thiago S. [Observatorio do Valongo, Universidade Federal do Rio de Janeiro, Ladeira Pedro Antonio 43, Saude, Rio de Janeiro-RJ, CEP 22240-060 (Brazil); Fragos, Tassos [Harvard-Smithsonian Center for Astrophysics, 60 Garden Street, Cambridge, MA 02138 (United States); Heckman, Timothy M. [Department of Physics and Astronomy, Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218 (United States); Overzier, Roderik A. [Department of Astronomy, University of Texas at Austin, 1 University Station C1400, Austin, TX 78712 (United States); Schiminovich, David, E-mail: antara.r.basu-zych@nasa.gov [Department of Astronomy, Columbia University, 550 West 120th Street, New York, NY 10027 (United States)

2013-09-10T23:59:59.000Z

469

Helical Disruptions in Small Loops of DNA  

E-Print Network [OSTI]

The thermodynamical stability of DNA minicircles is investigated by means of path integral techniques. Hydrogen bonds between base pairs on complementary strands can be broken by thermal fluctuations and temporary fluctuational openings along the double helix are essential to biological functions such as transcription and replication of the genetic information. Helix unwinding and bubble formation patterns are computed in circular sequences with variable radius in order to analyze the interplay between molecule size and appearance of helical disruptions. The latter are found in minicircles with $100$ base pairs and appear as a strategy to soften the stress due to the bending and torsion of the helix.

Zoli, Marco

2015-01-01T23:59:59.000Z

470

DNA Origami: A History and Current Perspective  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr May JunDatastreamsmmcrcalgovInstrumentsruc DocumentationP-Series to UserProduct: Crude OilPublicDNA Gridiron Nanostructures

471

Intriguing DNA Editor Has a Structural Trigger  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmospheric Optical Depth7-1D: Vegetation ProposedUsingFunInfrared LandResponses to EngineeredA GENERAL2RadiativeIntriguing DNA Editor

472

Intriguing DNA Editor Has a Structural Trigger  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmospheric Optical Depth7-1D: Vegetation ProposedUsingFunInfrared LandResponses to EngineeredA GENERAL2RadiativeIntriguing DNA

473

Isolation of Discrete Nanoparticle-DNA Conjugates for Plasmonic Applications  

SciTech Connect (OSTI)

Discrete DNA-gold nanoparticle conjugates with DNA lengths as short as 15 bases for both 5 nm and 20 nm gold particles have been purified by anion-exchange HPLC. Conjugates comprising short DNA (<40 bases) and large gold particles (>_ 20 nm) are difficult to purify by other means, and are potential substrates for plasmon coupling experiments. Conjugate purity is demonstrated by hybridizing complementary conjugates to form discrete structures, which are visualized by TEM.

Alivisatos, Paul; Claridge, Shelley A.; Liang, Huiyang W.; Basu, Sourav Roger; Frechet, Jean M.J.; Alivisatos, A. Paul

2008-04-11T23:59:59.000Z

474

Alternative Methods for Human Identification: Mitochondrial DNA Base Composition Profiling  

E-Print Network [OSTI]

Profiling by ESI-TOF Mass Spectrometry Kevin Kiesler Research Biologist, Applied Genetics Group Forensics · Why use Mass Spectrometry? · Abbott / Ibis Biosciences PLEX-ID Instrument · PLEX-ID mtDNA 2.0 Assay://remf.dartmouth.edu/images/mammalianLungTEM/source/8.html #12;Applied Genetics mtDNA Genotyping for Human I.D. · Mutations in mtDNA occur naturally

Perkins, Richard A.

475

Modeling DNA Interactions in Nucleosome Particles (A Computational Study of DNA Interactions in Nucleosome Particles)  

E-Print Network [OSTI]

Technology Center, University of Kansas, Lawrence, KS 66045. 2006 Annual Kansas City Area Life Sciences characterize global motions of the histone-DNA complex with respect to energy fluctuations in an assessment of energy differences resulting from the nucleotide substitutions will be presented in the context

Kansas, University of

476

Regulation of DNA Repair Fidelity by Molecular Checkpoints: "Gates" in DNA Polymerase 's Substrate Selection  

E-Print Network [OSTI]

Schlick*, Department of Chemistry and Courant Institute of Mathematical Sciences, New York UniVersity, New York, New York 10012, and Laboratory of Structural Biology, National Institute of EnVironmental Health enzymology data. Focusing on DNA polymerase (pol ), we present an emerging view of the geometric, energetic

Schlick, Tamar

477

assemble linear dna: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

compute? In his early studies of reversible computation, Bennett imagined an enzymatic Turing Machine which modified a hetero-polymer (such as DNA) to perform computation with...

478

assembly regulate dna: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

compute? In his early studies of reversible computation, Bennett imagined an enzymatic Turing Machine which modified a hetero-polymer (such as DNA) to perform computation with...

479

Fleet DNA Project - Data Dictionary for Public Download Files  

SciTech Connect (OSTI)

Reference document for the Fleet DNA results data shared on the NREL public website. The document includes variable definitions and descriptions to assist users in understanding data.

Duran, A.; Burton, E.; Kelly, K.; Walkowicz, K.

2014-09-01T23:59:59.000Z

480

Topoisomerase II Structure Suggests Novel DNA Cleavage Mechanism  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

doubly-wound polymer, processes such as chromosome compaction and replication naturally lead to DNA overwinding, underwinding, and tangling. Topoisomerases are essential to...

Note: This page contains sample records for the topic "dna double-strand breaks" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


481

DNA-inspired materials for 'bottom-up' nanotechnology.  

E-Print Network [OSTI]

??DNA is a remarkable material that is both an inspiration for polymer nanotechnology and a versatile building block for assembling well-defined nanostructures. To create polymeric… (more)

Ishihara, Yoshihiro.

2007-01-01T23:59:59.000Z

482

Ergonomics in DNA Sequencing: Standing Down to Ergonomics  

E-Print Network [OSTI]

Ergonomics in DNA SequencingStanding Down to Ergonomics Presented at the COEH 2009We Do: Introduction • How We Work: Ergonomics Challenges in

Naca, Christine

2009-01-01T23:59:59.000Z

483

Nanoscale topographical replication of graphene architecture by artificial DNA nanostructures  

SciTech Connect (OSTI)

Despite many studies on how geometry can be used to control the electronic properties of graphene, certain limitations to fabrication of designed graphene nanostructures exist. Here, we demonstrate controlled topographical replication of graphene by artificial deoxyribonucleic acid (DNA) nanostructures. Owing to the high degree of geometrical freedom of DNA nanostructures, we controlled the nanoscale topography of graphene. The topography of graphene replicated from DNA nanostructures showed enhanced thermal stability and revealed an interesting negative temperature coefficient of sheet resistivity when underlying DNA nanostructures were denatured at high temperatures.

Moon, Y.; Seo, S.; Park, J.; Park, T.; Ahn, J. R., E-mail: jrahn@skku.edu [Department of Physics, Sungkyunkwan University, Suwon 440-746 (Korea, Republic of); Shin, J.; Dugasani, S. R. [Sungkyunkwan Advanced Institute of Nanotechnology (SAINT), Sungkyunkwan University, Suwon 440-746 (Korea, Republic of); Woo, S. H. [College of Pharmacy, Chungnam National University, Daejeon 305-764 (Korea, Republic of); Park, S. H., E-mail: sunghapark@skku.edu [Department of Physics, Sungkyunkwan University, Suwon 440-746 (Korea, Republic of); Sungkyunkwan Advanced Institute of Nanotechnology (SAINT), Sungkyunkwan University, Suwon 440-746 (Korea, Republic of)

2014-06-09T23:59:59.000Z

484

Solvent resistant microfluidic DNA synthesizer{ Yanyi Huang,{a  

E-Print Network [OSTI]

a microfluidic DNA synthesizer out of perfluoropolyether (PFPE), an elastomer with excellent chemical characterization of the products.6 We previously reported a photocurable perfluoropolyether (PFPE) elastomer, which

Huang, Yanyi

485

High-Performance Integrated Genetic Analyzers for Forensic DNA Typing  

E-Print Network [OSTI]

Encoded evidence: DNA in forensic analysis. Nature Reviewspopulations. Journal of Forensic Sciences 48, 908-911 (identity testing. Journal of Forensic Sciences 51, 253-265 (

Liu, Peng

2009-01-01T23:59:59.000Z

486

Ergodicity breaking and wave-function statistics in disordered interacting systems  

SciTech Connect (OSTI)

We present the study of the structure of many-body eigenfunctions in a one-dimensional disordered spin chain. We discuss the choice of an appropriate basis in the Hilbert space, where the problem can be seen as an Anderson model defined on a high-dimensional non-trivial graph, determined by the many-body Hamiltonian. The comparison with the usual behavior of wave-functions in finite dimensional Anderson localization allows us to put in light the main differences of the many-body case. At high disorder, the typical eigenfunctions do not seem to localize though they occupy a infinitesimal portion of the Hilbert space in the thermodynamic limit. We perform a detailed analysis of the distribution of the wave-function coefficients and their peculiar scaling in the small and large disorder phase. We propose a criterion to identify the position of the transition by looking at the long tails of these distributions. The results coming from exact diagonalization show signs of breaking of ergodicity when the disorder reaches a critical value that agrees with the estimation of the many-body localization transition in the same model.

De Luca, Andrea [Laboratoire de Physique Théorique de l'ENS and Institut de Physique Theorique Philippe Meyer (France)

2014-08-20T23:59:59.000Z

487

Analysis of a Main Steam Line Break in Asco Nuclear Power Plant  

SciTech Connect (OSTI)

A comprehensive analysis of a double-ended main steam line break (MSLB) accident assumed to occur in the Asco nuclear power plant was carried out using the RELAP/PARCS coupled code. The general results of the benchmark provide a certain qualification of tools and methodologies used. Applying such methodologies to other plant models can be useful to extend conclusions and to identify areas where further analysis is needed. The calculations showed the capability of the control rod to recover the accident. However, one stuck control rod caused some recriticality or return to power (RTP), whose magnitude is heavily affected by the initial and boundary conditions. This paper identifies similarities and discrepancies between the benchmark calculation on the TMI-1 model and the Westinghouse three-loop calculation on the Asco model. The use of an integral plant model was helpful in showing the importance on the RTP of different plant systems that are modeled in detail. The high-pressure injection system and feedwater lines as well as the broken steam line model are the most significant.

Cuadra, Arantxa; Gago, Jose Luis; Reventos, Francesc

2001-06-17T23:59:59.000Z

488

Shifted focus point of the Higgs mass parameter from the minimal mixed mediation of SUSY breaking  

E-Print Network [OSTI]

We employ both the minimal gravity- and the minimal gauge mediations of supersymmetry breaking at the grand unified theory scale (GUT) in a single supergravity framework. In such a "minimal mixed mediation model," a "focus point" of the Higgs mass parameter, m_{h_u}^2 emerges at 3-4 TeV energy scale, which is exactly the stop mass scale needed for explaining the 126 GeV Higgs boson mass without the "A-term" at the three loop level. As a result, m_{h_u}^2 can be quite insensitive to various trial stop masses at low energy, reducing the fine-tuning measures to be much smaller than 100 even for 3-4 TeV low energy stop mass and -0.5 < A_t / m_0 < 0 at the GUT scale. In this model the gluino mass is predicted to be about 2.3 TeV, which could readily be tested at LHC run 2.

Kyae, Bumseok

2015-01-01T23:59:59.000Z

489

A dimension-breaking phenomenon for water waves with weak surface tension  

E-Print Network [OSTI]

It is well known that the water-wave problem with weak surface tension has small-amplitude line solitary-wave solutions which to leading order are described by the nonlinear Schr\\"odinger equation. The present paper contains an existence theory for three-dimensional periodically modulated solitary-wave solutions which have a solitary-wave profile in the direction of propagation and are periodic in the transverse direction; they emanate from the line solitary waves in a dimension-breaking bifurcation. In addition, it is shown that the line solitary waves are linearly unstable to long-wavelength transverse perturbations. The key to these results is a formulation of the water wave problem as an evolutionary system in which the transverse horizontal variable plays the role of time, a careful study of the purely imaginary spectrum of the operator obtained by linearising the evolutionary system at a line solitary wave, and an application of an infinite-dimensional version of the classical Lyapunov centre theorem.

Mark D. Groves; Shu-Ming Sun; Erik Wahlén

2014-11-10T23:59:59.000Z

490

Confinement, quark mass functions, and spontaneous chiral symmetry breaking in Minkowski space  

SciTech Connect (OSTI)

We formulate the covariant equations for quark-antiquark bound states in Minkowski space in the framework of the Covariant Spectator Theory. The quark propagators are dressed with the same kernel that describes the interaction between different quarks. We show that these equations are charge conjugation invariant, and that in the chiral limit of vanishing bare quark mass, a massless pseudoscalar bound state is produced in a Nambu--Jona-Lasinio (NJL) mechanism, which is associated with the Goldstone boson of spontaneous chiral symmetry breaking. In this introductory paper we test the formalism by using a simplified kernel consisting of a momentum-space $\\delta$-function with a vector Lorentz structure, to which one adds a mixed scalar and vector confining interaction. The scalar part of the confining interaction is not chirally invariant by itself, but decouples from the equations in the chiral limit and therefore allows the NJL mechanism to work. With this model we calculate the quark mass function, and we compare our Minkowski-space results to LQCD data obtained in Euclidean space. In a companion paper we apply this formalism to a calculation of the pion form factor.

Biernat, Elmar P. [CFTP, Institute Superior Tecnico, Lisboa (Portugal); Gross, Franz L. [JLAB, Newport News, VA (United States); Pena, Teresa [CFTP, Institute Superior Tecnico, Lisboa (Portugal); Stadler, Alfred [University of Evora, Lisboa (Portugal)

2014-01-01T23:59:59.000Z

491

Breaking the EOS-Gravity Degeneracy with Masses and Pulsating Frequencies of Neutron Stars  

E-Print Network [OSTI]

A thorough understanding of many astrophysical phenomena associated with compact objects requires reliable knowledge about both the equation of state (EOS)of super-dense nuclear matter and the theory of strong-field gravity simultaneously because of the EOS-gravity degeneracy. Currently, variations of the neutron star (NS) mass-radius correlation from using alternative gravity theories are much larger than those from changing the NS matter EOS within known constraints. At least two independent observables are required to break the EOS-gravity degeneracy. Using model EOSs for hybrid stars and a Yukawa-type non-Newtonian gravity, we investigate both the mass-radius correlation and pulsating frequencies of NSs. While the maximum mass of NSs increases, the frequencies of the $f$, $p_1$, $p_2$, and $w_I$ pulsating modes are found to decrease with the increasing strength of the Yukawa-type non-Newtonian gravity, providing a useful reference for future determination simultaneously of both the strong-field gravity and the supranuclear EOS by combining data of x-ray and gravitational wave emissions of neutron stars.

Weikang Lin; Bao-An Li; Lie-Wen Chen; De-Hua Wen; Jun Xu

2014-03-21T23:59:59.000Z

492

Spontaneous symmetry breaking in 2D: Kibble-Zurek mechanism in temperature quenched colloidal monolayers  

E-Print Network [OSTI]

The Kibble-Zurek mechanism describes the formation of topological defects during spontaneous symmetry breaking for quite different systems. Shortly after the big bang, the isotropy of the Higgs-field is broken during the expansion and cooling of the universe. Kibble proposed the formation of monopoles, strings, and membranes in the Higgs field since the phase of the symmetry broken field can not switch globally to gain the same value everywhere in space. Zurek pointed out that the same mechanism is relevant for second order phase transitions in condensed matter systems. Every finite cooling rate induces the system to fall out of equilibrium which is due to the critical slowing down of order parameter fluctuations: the correlation time diverges and the symmetry of the system can not change globally but incorporates defects between different domains. Depending on the cooling rate the heterogeneous order parameter pattern are a fingerprint of critical fluctuations. In the present manuscript we show that a monolayer of superparamagnetic colloidal particles is ideally suited to investigate such phenomena. In thermal equilibrium the system undergos continuous phase transitions according KTHNY-theory. If cooled rapidly across the melting temperature the final state is a polycrystal. We show, that the observations can not be explained with nucleation of a supercooled fluid but is compatible with the Kibble-Zurek mechanism.

Patrick Dillmann; Georg Maret; Peter Keim

2014-09-16T23:59:59.000Z

493

Proceedings of the seminar on leak before break in reactor piping and vessels  

SciTech Connect (OSTI)

The objective of the seminar was to present the current state of the art in Leak-Before-Break (LBB) methodology development, validation, and application in an international forum. With particular emphasis on industrial applications and regulatory policies, the seminar provided an opportunity to compare approaches, experiences, and codifications developed by different countries. The seminar was organized into four topic areas: status of LBB applications; technical issues in LBB methodology; complementary requirements (leak detection and inspection); LBB assessment and margins. As a result of this seminar, an improved understanding of LBB gained through sharing of different viewpoints from different countries, permits consideration of: simplified pipe support design and possible elimination of loss-of-coolant-accident (LOCA) mechanical consequences for specific cases; defense-in-depth type of applications without support modifications; support of safety cases for plants designed without the LOCA hypothesis. In support of these activities, better estimates of the limits to the LBB approach should follow, as well as an improvement in codifying methodologies. Selected papers are indexed separately for inclusion in the Energy Science and Technology Database.

Faidy, C. [ed.] [Electricite de France, Villeurbanne (France); Gilles, P. [ed.] [Framatome, Paris (France)

1997-04-01T23:59:59.000Z

494

Leak before break behaviour of austenitic and ferritic pipes containing circumferential defects  

SciTech Connect (OSTI)

Several research projects carried out at MPA Stuttgart to investigate the Leak-before-Break (LBB) behavior of safety relevant pressure bearing components are summarized. Results presented relate to pipes containing circumferential defects subjected to internal pressure and external bending loading. An overview of the experimentally determined results for ferritic components is presented. For components containing postulated or actual defects, the dependence of the critical loading limit on the defect size is shown in the form of LBB curves. These are determined experimentally and/or by calculation for through-wall slits, and represent the boundary curve between leakage and massive fracture. For surface defects and a given bending moment and internal pressure, no fracture will occur if the length at leakage remains smaller than the critical defect length given by the LBB curve for through-wall defects. The predictive capability of engineering calculational methods are presented by way of example. The investigation programs currently underway, testing techniques, and initial results are outlined.

Stadtmueller, W.; Sturm, D.

1997-04-01T23:59:59.000Z

495

Spontaneous breaking of conformal invariance, solitons, and gravitational waves in theories of conformally invariant gravitation  

SciTech Connect (OSTI)

We study conformal gravity as an alternative theory of gravitation. For conformal gravity to be phenomenologically viable requires that the conformal symmetry is not manifest at the energy scales of the other known physical forces. Hence we are required to find a mechanism for the spontaneous breaking of conformal invariance. In this paper we study the possibility that conformal invariance is spontaneously broken due to interactions with conformally coupled matter fields. The vacuum of the theory admits conformally noninvariant solutions corresponding to maximally symmetric space-times and variants thereof. These are either de Sitter space-time or anti-de Sitter space-time in the full four space-time dimensions and we find new solutions corresponding to maximal symmetry restricted to a lower dimensional subspace. We also consider normalizable, linearized gravitational perturbations around the anti-de Sitter background. We show to second order, that these gravitational fluctuations carry zero energy momentum. Finally we also show the possibility of domain wall solitons interpolating between the ground states of spontaneously broken conformal symmetry that we have found. These solitons necessarily require the vanishing of the scalar field. This offers a way of eschewing the recent suggestion and its consequences [E. Flanagan, Phys. Rev. D 74, 023002 (2006).] that the conformal symmetry could be quarantined to a sterile sector of the theory by choosing an appropriate field redefinition.

Bouchami, Jihene [Groupe de physique des particules, Departement de physique, Universite de Montreal, Case Postale 6128, succursale Centre-ville, Montreal, Quebec, H3C 3J7 (Canada); Paranjape, M. B. [Groupe de physique des particules, Departement de physique, Universite de Montreal, Case Postale 6128, succursale Centre-ville, Montreal, Quebec, H3C 3J7 (Canada); Center for Quantum Spacetime, Department of Physics, Sogang University, Shinsu-dong 1, Mapo-gu, Seoul, 121-742 (Korea, Republic of)

2008-08-15T23:59:59.000Z

496

Spontaneous R-Parity Breaking, Stop LSP Decays and the Neutrino Mass Hierarchy  

E-Print Network [OSTI]

The MSSM with right-handed neutrino supermultiplets, gauged B-L symmetry and a non-vanishing sneutrino expectation value is the minimal theory that spontaneously breaks R-parity and is consistent with the bounds on proton stability and lepton number violation. This minimal B-L MSSM can have a colored/charged LSP, of which a stop LSP is the most amenable to observation at the LHC. We study the R-parity violating decays of a stop LSP into a bottom quark and charged leptons--the dominant modes for a generic "admixture" stop. A numerical analysis of the relative branching ratios of these decay channels is given using a wide scan over the parameter space. The fact that R-parity is violated in this theory by a vacuum expectation value of a sneutrino links these branching ratios directly to the neutrino mass hierarchy. It is shown how a discovery of bottom-charged lepton events at the LHC can potentially determine whether the neutrino masses are in a normal or inverted hierarchy, as well as determining the theta_23 neutrino mixing angle. Finally, present LHC bounds on these leptoquark signatures are used to put lower bounds on the stop mass.

Zachary Marshall; Burt A. Ovrut; Austin Purves; Sogee Spinner

2014-06-03T23:59:59.000Z

497

Improved calculation of the isospin-symmetry-breaking corrections to superallowed Fermi beta decay  

E-Print Network [OSTI]

We report new shell-model calculations of the isospin-symmetry-breaking correction delta(C) to superallowed 0(+)-> 0(+) nuclear beta decay. The most important improvement is the inclusion of core orbitals, which are demonstrated to have a...this leads to small changes in delta(NS) as well. We also examine the new radiative-correction calculation by Marciano and Sirlin and, by a simple reorganization, show that it is possible to preserve the conventional separation into a nucleus-independent "inner" radiative term, Delta(V)(R), and a nucleus-dependent "outer" term, delta(')(R). We tabulate the new values for delta(C),delta(NS), and delta(')(R) for 20 superallowed transitions, including the 13 currently well-studied cases. With these new correction terms, the corrected Ft values for the 13 cases are statistically consistent with one another, and the anomalousness of the (46)V result disappears. These new calculations lead to a lower average (Ft) over bar value and a higher value for V(ud). The sum of squares of the top-row elements of the Cabibbo-Kobayashi-Maskawa matrix now agrees exactly with unitarity....

Towner, I. S.; Hardy, John C.

2008-01-01T23:59:59.000Z

498

The effect of symmetry breaking on the dynamics near a structurally stable heteroclinic cycle between equilibria and a periodic orbit  

E-Print Network [OSTI]

The effect of small forced symmetry breaking on the dynamics near a structurally stable heteroclinic cycle connecting two equilibria and a periodic orbit is investigated. This type of system is known to exhibit complicated, possibly chaotic dynamics including irregular switching of sign of various phase space variables, but details of the mechanisms underlying the complicated dynamics have not previously been investigated. We identify global bifurcations that induce the onset of chaotic dynamics and switching near a heteroclinic cycle of this type, and by construction and analysis of approximate return maps, locate the global bifurcations in parameter space. We find there is a threshold in the size of certain symmetry-breaking terms below which there can be no persistent switching. Our results are illustrated by a numerical example.

Vivien Kirk; Alastair M. Rucklidge

2008-05-07T23:59:59.000Z

499

IJOPE Paper No JC-343AB-final 9/26/04 Biausser Page number 1 Numerical Analysis of the Internal Kinematics and Dynamics of 3D Breaking Waves on Slopes  

E-Print Network [OSTI]

Kinematics and Dynamics of 3D Breaking Waves on Slopes Benjamin Biausser1 , Stéphan T. Grilli2 , Philippe and internal kinematics (velocity, vorticity, pressure) are presented. KEY WORDS: breaking ocean waves years, our understanding of wave breaking dynamics and kinematics is still quite incomplete. Due

Grilli, Stéphan T.

500

ATP AND DNA AS MICROBIAL PARAMETERS IN THE ALIMENTARY TRACT  

E-Print Network [OSTI]

ATP AND DNA AS MICROBIAL PARAMETERS IN THE ALIMENTARY TRACT J. WOLSTRUP K. JENSEN A. JUST I. The aim of the present work was to investi- gate the use of ATP and DNA as parameters for microbial activity and biomass in the ali- mentary tract of cows and pigs. ATP was selected because of the promising

Paris-Sud XI, Université de