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1

Recognition of double helical DNA by alternate strand triple helix formation  

SciTech Connect (OSTI)

The triplet specificities and required strand orientations of two classes of DNA triple helices can be combined to target double helical sequences containing all four base pairs by alternate strand triple helix formation. This allows for the use of oligonucleotides containing only natural 3{prime}-5{prime} phosphodiester linkages to simultaneously bind both strands of double helical DNA in the major groove. The stabilities and structures of these alternate strand triple helices depend on whether the binding site sequences is 5{prime}-(purine){sub m}(pyrimidine){sub n}-3{prime} or 5{prime}-(pyrimidine){sub m}(purine){sub n}-3{prime}. The sequence type 5{prime}-(purine){sub m}- (pyrimidine){sub n}-3{prime} was targeted with an oligonucleotide consisting of a pyrimidine domain and a purine domain linked by a 3{prime}-5{prime} phosphodiester. To bind the duplex sequence type 5{prime}-(pyrimidine){sub m}(purine){sub n}-3{prime}, the third strand requires at least two nucleotides linking binding domains at the site of crossover in the major groove. A new class of oligonucleotides capable of binding mixed duplex sequences by crossovers in the major groove may be possible. 15 refs., 13 figs.

Beal, P.A.; Dervan, P.B. [California Institute of Technology, Pasadena, CA (United States)

1992-06-17T23:59:59.000Z

2

Double Helix  

E-Print Network [OSTI]

...a pair of wings...an axe, and a syrinx? (5). Here we see a possible transition from inscribing poems on actual objects ? such as altars ? to creating abstract representations of objects out of letters. 6 Higgins notes that no examples of Classical......a pair of wings...an axe, and a syrinx? (5). Here we see a possible transition from inscribing poems on actual objects ? such as altars ? to creating abstract representations of objects out of letters. 6 Higgins notes that no examples of Classical...

Stumpo, Jeffrey David

2012-07-16T23:59:59.000Z

3

Spin transport and spin polarization properties in double-stranded DNA  

SciTech Connect (OSTI)

We study the spin-dependent electron transport through a double-stranded DNA (dsDNA) using the Bogoliubov-de Gennes equations and non-equilibrium Green's function method. We calculate the spin-dependent electron conductance and spin-polarization for different lengths, helix angles, twist angles of dsDNA, the environment-induced dephasing factors, and hopping integral. It is shown that the conductance decreases by increasing the length and dephasing factor. Also, we show that the spin-polarization depends on the helical symmetry and the length of DNA. It is shown that the double-stranded DNA can act as a perfect spin filter. Finally, we show that the sign of spin polarization can be inverted from +1 (?1) to ?1 (+1) for some values of hopping integral.

Simchi, Hamidreza, E-mail: simchi@iust.ac.ir [Department of Physics, Iran University of Science and Technology, Narrmak, Tehran 16844 (Iran, Islamic Republic of); Semiconductor Technology Center, Tehran (Iran, Islamic Republic of); Esmaeilzadeh, Mahdi, E-mail: mahdi@iust.ac.ir; Mazidabadi, Hossein [Department of Physics, Iran University of Science and Technology, Narrmak, Tehran 16844 (Iran, Islamic Republic of)

2013-11-21T23:59:59.000Z

4

Repertoire Selection of Variant Single-Chain Cro:? Toward Directed DNA-Binding Specificity of Helix?Turn?Helix Proteins  

Science Journals Connector (OSTI)

Utilizing phage display-afforded affinity selection, scCro variants have been isolated for binding to synthetic DNA ligands. ... Variant proteins with altered DNA-sequence specificity were identified, which favored binding of targeted synthetic DNA sequences over a consensus operator sequence, bound with high affinity by wild-type Cro. ... The specificities were relatively modest (2?3-fold, as calculated from KD values), which can be attributed to the inherent properties in the design of the selection system; one half-site of the synthetic DNA sequences maintains the consensus operator sequence, and one “subunit” of the variant single-chain Cro dimers was conserved as wild-type sequence. ...

Mikael T. I. Nilsson; Mikael Widersten

2004-08-28T23:59:59.000Z

5

Agrobacterium tumefaciens VirC2 enhances T-DNA transfer and virulence through its C-terminal ribbon–helix–helix DNA-binding fold  

Science Journals Connector (OSTI)

...extending DNA strand separation to the nick site at the origin of transfer . Mol Microbiol...Z-score of 11.3 and a mean figure of merit of 0.37. Maximum likelihood density...phases, yielding an overall figure of merit of phasing of 0.66. Automatic building...

Jun Lu; Amke den Dulk-Ras; Paul J. J. Hooykaas; J. N. Mark Glover

2009-01-01T23:59:59.000Z

6

Helix coupling  

DOE Patents [OSTI]

A coupling for connecting helix members in series, which consists of a pair of U-shaped elements, one of which is attached to each helix end with the U sections of the elements interlocked. The coupling is particularly beneficial for interconnecting helical Nitinol elements utilized in thermal actuators or engines. Each coupling half is attached to the associated helix at two points, thereby providing axial load while being easily removed from the helix, and reusable.

Ginell, W.S.

1982-03-17T23:59:59.000Z

7

SELF-ASSEMBLED DNA NANOSTRUCTURES AND DNA-TEMPLATED SILVER NANOWIRES  

E-Print Network [OSTI]

helix bundles, and the cross-tiles as well as synthetic double-stranded DNA moleculesSELF-ASSEMBLED DNA NANOSTRUCTURES AND DNA-TEMPLATED SILVER NANOWIRES by Sung Ha Park Department;ABSTRACT SELF-ASSEMBLED DNA NANOSTRUCTURES AND DNA-TEMPLATED SILVER NANOWIRES by Sung Ha Park Department

Reif, John H.

8

Quantitative, non-invasive imaging of radiation-induced DNA double strand breaks in vivo  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Quantitative, non-invasive imaging of radiation-induced DNA double strand breaks in vivo Quantitative, non-invasive imaging of radiation-induced DNA double strand breaks in vivo Wenrong Li 1, , Fang Li 1 , Qian Huang 1 , Jingping Shen 1 , Frank Wolf 1 , Yujun He 1 , Xinjian Liu 1 , Y. Angela Hu 1 , Joel. S. Bedford 5 , and Chuan-Yuan Li 1,2,* Departments of 1 Radiation Oncology, 2 Pharmacology, University of Colorado School of Medicine, Aurora, Colorado, USA; 3 Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, CO, USA DNA double strand breaks are a major form of DNA damage and a key mechanism through which radiotherapy and some chemotherapeutic agents kill cancer cells. Despite its importance, measuring DNA double strand breaks is still a tedious task that is normally carried out by gel electrophoresis or immunofluorescence staining. Here we report a novel approach to image and

9

ASSESSMENT OF INDIVIDUAL VARIATION IN DNA DOUBLE-STRAND BREAK REPAIR CAPACITY IN HUMAN DIPLOID FIBROBLASTS  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

ASSESSMENT OF INDIVIDUAL VARIATION IN DNA DOUBLE-STRAND ASSESSMENT OF INDIVIDUAL VARIATION IN DNA DOUBLE-STRAND BREAK REPAIR CAPACITY IN HUMAN DIPLOID FIBROBLASTS Paul F. Wilson, John M. Hinz, Peter B. Nham, Salustra S. Urbin, Cynthia B. Thomas, Irene M. Jones, and Larry H. Thompson Biosciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA The induction of bi-stranded clustered DNA damage (BCD), which includes direct DNA double-strand breaks (DSBs), is a hallmark of ionizing radiation (IR) exposure. Incorrectly repaired DSBs can cause chromosomal rearrangements and an increased risk of genomic instability and cancer. Because there is polymorphic variation in DNA repair genes and much of this variation is predicted to have a functional impact, healthy people likely vary in their capacity to repair DSBs and other BCD. This project

10

Sequence Specific Recognition of Double Helical DNA. A Synthetic Approach  

Science Journals Connector (OSTI)

The design of synthetic sequence-specific DNA-binding molecules has advanced in recent years ... rapid and precise analyses of hundreds of potential DNA-binding sites to nucleotide resolution on sequencing ... bi...

P. B. Dervan

1988-01-01T23:59:59.000Z

11

Structure determination and analysis of helix parameters in the DNA decamer d(CATGGCCATG)2 comparison of results from NMR and crystallography  

Science Journals Connector (OSTI)

The solution structure of the DNA decamer (CATGGCCATG)2 has been determined by NMR spectroscopy and restrained molecular dynamic and distance geometry calculations. The restrainted data set includes interproton distances and torsion angles for the deoxyribose sugar ring which were obtained by nuclear Overhauser enhancement intensities and quantitative simulation of cross-peaks from double quantum filtered correlation spectroscopy. The backbone torsion angles were constrained using experimental data from NOE cross-peaks, 1H-1H and 1H-31P-coupling constants. The NMR structure and the crystal structure of the DNA decamer deviates from the structure of the canonical form of B-DNA in a number of observable characteristics. Particularly, both structures display a specific pattern of stacking interaction in the central GGC base triplet. Furthermore, a specific local conformation of the TG/CA base-pair step is present in NMR and crystal structure, highlighting the unusually high flexibility of this DNA duplex part. The solution structure of the TG/CA base-pair step obtained by our high resolution NMR study is characterized by a positive roll angle, whereas in crystal this base-pair step tends to adopt remarkably high twist angles.

Utz Dornberger; Joachim Flemming; Hartmut Fritzsche

1998-01-01T23:59:59.000Z

12

The Role of DNA double-strand break repair in cellular response to low  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

DNA double-strand break repair in cellular response to low DNA double-strand break repair in cellular response to low dose radiation exposure. David J. Chen Division of Molecular Radiation Biology, Department of Radiation Oncology, University of Texas Southwestern Medical Center, Dallas TX 75390 It has been assumed that molecular pathways that involved in the biological response for low dose of radiation should be similar to those for high dose radiation in general. The low dose-rate effect and DNA double-strand break repair are inextricably linked in mammalian cells. It has been reported that mammalian mutant cells deficient in nonhomologous end join (NHEJ) pathway have little or no cellular recovery when expose to low-dose-rate radiation. Recently, it has also been reported that cells deficient in

13

DNA hydration studied by neutron fiber diffraction  

SciTech Connect (OSTI)

The development of neutron high angle fiber diffraction to investigate the location of water around the deoxyribonucleic acid (DNA) double-helix is described. The power of the technique is illustrated by its application to the D and A conformations of DNA using the single crystal diffractometer, D19, at the Institute Laue-Langevin, Grenoble and the time of flight diffractometer, SXD, at the Rutherford Appleton ISIS Spallation Neutron Source. These studies show the existence of bound water closely associated with the DNA. The patterns of hydration in these two DNA conformations are quite distinct and are compared to those observed in X-ray single crystal studies of two-stranded oligodeoxynucleotides. Information on the location of water around the DNA double-helix from the neutron fiber diffraction studies is combined with that on the location of alkali metal cations from complementary X-ray high angle fiber diffraction studies at the Daresbury Laboratory SRS using synchrotron radiation. These analyses emphasize the importance of viewing DNA, water and ions as a single system with specific interactions between the three components and provide a basis for understanding the effect of changes in the concentration of water and ions in inducing conformations] transitions in the DNA double-helix.

Fuller, W.; Forsyth, V.T.; Mahendrasingam, A.; Langan, P.; Pigram, W.J. [Keele Univ. (United Kingdom)] [and others

1994-12-31T23:59:59.000Z

14

Gene Expression Pathways Activated and Repair of DNA of Double Strand  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Pathways Activated and Repair of DNA of Double Strand Pathways Activated and Repair of DNA of Double Strand Breaks (DSBs) Following Low Dose Ionizing Radiation in Human Bronchial Epithelial Cells (HBECs) With and Without Defined Premalignant Oncogenic Changes John Minna University of Texas Southwestern Medical Center at Dallas Abstract We want to determine the effect of low dose ionizing radiation on the molecular progression of lung epithelial cells towards lung cancer. Our endpoints are focused on specific molecular and cellular changes known to be associated with lung cancer development and quantitating those changes as a function of low dose ionizing radiation. We use immortalized human bronchial epithelial cells (HBECs) developed from >40 individuals (including those from current, former, and never smokers, males, females,

15

A Key Step in Repairing DNA Double-Strand Breaks | Advanced Photon Source  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

X-ray Rainbow X-ray Rainbow An Insulating Breakthrough Science Highlights Archives: 2013 | 2012 | 2011 | 2010 2009 | 2008 | 2007 | 2006 2005 | 2004 | 2003 | 2002 2001 | 2000 | 1998 | Subscribe to APS Science Highlights rss feed A Key Step in Repairing DNA Double-Strand Breaks JANUARY 10, 2007 Bookmark and Share The atomic structure of the protein 53BP1 identified by Mayo researchers. (Courtesy: Mayo Clinic) A team of Mayo Clinic researchers has uncovered a key step in the molecular pathway of repairing DNA double-strand breaks. The studies were carried out using the 19-ID beamline (SBC-CAT) at the Advanced Photon Source (APS, Argonne) and the X12-C beamline at the National Synchrotron Light Source (NSLS, Brookhaven National Laboratory). Both the APS and the NSLS are funded by the U.S. Department of Energy's Office of Basic Energy

16

Probability of double-strand breaks in genome-sized DNA by {gamma}-ray decreases markedly as the DNA concentration increases  

SciTech Connect (OSTI)

By use of the single-molecule observation, we count the number of DNA double-strand breaks caused by {gamma}-ray irradiation with genome-sized DNA molecules (166 kbp). We find that P{sub 1}, the number of double-strand breaks (DSBs) per base pair per unit Gy, is nearly inversely proportional to the DNA concentration above a certain threshold DNA concentration. The inverse relationship implies that the total number of DSBs remains essentially constant. We give a theoretical interpretation of our experimental results in terms of attack of reactive species upon DNA molecules, indicating the significance of the characteristics of genome-sized giant DNA as semiflexible polymers for the efficiency of DSBs.

Shimobayashi, Shunsuke F. [Department of Physics, Graduate School of Science, Kyoto University, Kyoto 606-8502 (Japan); Iwaki, Takafumi [Fukui Institute for Fundamental Chemistry, Kyoto University, Kyoto 606-8103 (Japan); Mori, Toshiaki [Radiation Research Center, Osaka Prefecture University, Sakai 599-8570 (Japan); Yoshikawa, Kenichi [Department of Physics, Graduate School of Science, Kyoto University, Kyoto 606-8502 (Japan); Faculty of Life and Medical Sciences, Doshisha University, Kyoto 610-0394 (Japan)

2013-05-07T23:59:59.000Z

17

Super D-helix  

Science Journals Connector (OSTI)

We study the “Myers effect” for a bunch of D1-branes with type-IIB superstrings moving in one direction along the branes. We show that the “blown-up” configuration is a helical D1-brane, which is self-supported from collapse by the axial momentum flow. The tilting angle of the helix is determined by the number of D1-branes. The radius of the helix is stabilized to a certain value depending on the number of D1-branes and the momentum carried by type-IIB superstrings. This helix is actually a T-dual version of the supertube recently found as the “blown-up” configuration of a bunch of type-IIA superstrings carrying a D0-brane charge. It is shown that the helical D1 configuration preserves one-quarter of the supersymmetry of the type-IIB Minkowski vacuum.

Jin-Ho Cho and Phillial Oh

2001-10-22T23:59:59.000Z

18

The Initiation of Bacterial DNA Replication  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

The Initiation of Bacterial DNA The Initiation of Bacterial DNA Replication The Initiation of Bacterial DNA Replication Print Wednesday, 31 January 2007 00:00 For the first time, scientists have determined the structure of the initiator of bacterial DNA replication. It is already known that such replication is controlled by a protein known as DnaA, a member of the AAA+ superfamily of ATPases. What has now been discovered is that the core of the initiator is not the closed-ring structure expected for this system. Instead, DnaA forms an open right-handed helix. In addition, the architecture indicates that this AAA+ superhelix will wrap coils of the DNA around its exterior, causing the DNA double helix to deform as a first step in the separation and unwinding of its strands. Eukaryotic and archaeal initiators also have the structural elements that promote open-helix formation, indicating that a spiral, open-ring AAA+ assembly is a conserved element from a common evolutionary ancestor of Archaea, Bacteria, and Eukarya.

19

The Initiation of Bacterial DNA Replication  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

The Initiation of Bacterial DNA Replication Print The Initiation of Bacterial DNA Replication Print For the first time, scientists have determined the structure of the initiator of bacterial DNA replication. It is already known that such replication is controlled by a protein known as DnaA, a member of the AAA+ superfamily of ATPases. What has now been discovered is that the core of the initiator is not the closed-ring structure expected for this system. Instead, DnaA forms an open right-handed helix. In addition, the architecture indicates that this AAA+ superhelix will wrap coils of the DNA around its exterior, causing the DNA double helix to deform as a first step in the separation and unwinding of its strands. Eukaryotic and archaeal initiators also have the structural elements that promote open-helix formation, indicating that a spiral, open-ring AAA+ assembly is a conserved element from a common evolutionary ancestor of Archaea, Bacteria, and Eukarya.

20

The Initiation of Bacterial DNA Replication  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

The Initiation of Bacterial DNA Replication Print The Initiation of Bacterial DNA Replication Print For the first time, scientists have determined the structure of the initiator of bacterial DNA replication. It is already known that such replication is controlled by a protein known as DnaA, a member of the AAA+ superfamily of ATPases. What has now been discovered is that the core of the initiator is not the closed-ring structure expected for this system. Instead, DnaA forms an open right-handed helix. In addition, the architecture indicates that this AAA+ superhelix will wrap coils of the DNA around its exterior, causing the DNA double helix to deform as a first step in the separation and unwinding of its strands. Eukaryotic and archaeal initiators also have the structural elements that promote open-helix formation, indicating that a spiral, open-ring AAA+ assembly is a conserved element from a common evolutionary ancestor of Archaea, Bacteria, and Eukarya.

Note: This page contains sample records for the topic "dna double helix" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


21

Proximity-induced superconductivity effect in a double-stranded DNA  

SciTech Connect (OSTI)

We study the proximity-induced superconductivity effect in a double-stranded DNA by solving the Bogoliubov-de Gennes equations and taking into account the effect of thermal fluctuations of the twist angle between neighboring base pairs. We show that the electron conductance is spin-dependent and the conductance of spin up (down) increases (decreases) due to the spin-orbit coupling (SOC). It is found that, for T?

Simchi, Hamidreza, E-mail: simchi@iust.ac.ir [Department of Physics, Iran University of Science and Technology, Narrmak, Tehran 16844 (Iran, Islamic Republic of); Semiconductor Technology Center, Tehran (Iran, Islamic Republic of); Esmaeilzadeh, Mahdi, E-mail: mahdi@iust.ac.ir; Mazidabadi, Hossein [Department of Physics, Iran University of Science and Technology, Narrmak, Tehran 16844 (Iran, Islamic Republic of)

2014-02-07T23:59:59.000Z

22

Examining the regulation of DNA end-processing at telomeres and double-strand breaks  

E-Print Network [OSTI]

do not exhibit synthetic growth or DNA damage responsedo not exhibit synthetic growth or DNA damage responsedo not exhibit synthetic growth or DNA damage response

Ballew, Bari Jane

2011-01-01T23:59:59.000Z

23

Telomeres and DNA double-strand breaks: ever the twain shall S. M. Baileya,* and M. N. Cornforthb  

E-Print Network [OSTI]

between the naturally occurring DNA double-strand breaks (DSBs) found at chromosome ends (i.e., telomeres Collins, CO 80523-1618 (USA), Fax: +19704917742, e-mail: sbailey@colostate.edu b Department of Radiation on their inability to rejoin with broken chromosome ends produced by radiation. Today, we recognize two essential

24

C-H..O Hydrogen Bonds in Minor Groove of A-tracts in DNA Double Helices  

E-Print Network [OSTI]

C-H..O Hydrogen Bonds in Minor Groove of A-tracts in DNA Double Helices Anirban Ghosh and Manju-pair as well as cross-strand C-H..O hydrogen bonds in the minor groove. The C2-H2..O2 hydrogen bonds within leads to a narrow minor groove in these regions. # 1999 Academic Press Keywords: C-H..O hydrogen bonds

Bansal, Manju

25

Repair of Site-Specific DNA Double-Strand Breaks in Barley Occurs via Diverse Pathways Primarily Involving the Sister Chromatid  

Science Journals Connector (OSTI)

...research-article RESEARCH ARTICLES Repair of Site-Specific DNA Double-Strand...various double-strand break repair pathways in the monocot plant...0.307_win32 with UPARSE pipeline (Edgar, 2013). The three...polymorphisms indicative of distinct repair mechanisms. SCE and Mitotic...

Giang T.H. Vu; Hieu X. Cao; Koichi Watanabe; Goetz Hensel; Frank R. Blattner; Jochen Kumlehn; Ingo Schubert

2014-05-29T23:59:59.000Z

26

Epigenetic Modifications in Double-Strand Break DNA Damage Signaling and Repair  

Science Journals Connector (OSTI)

...Focus 10 14 31 DNA Damage and Repair in Translational Oncology...investigators, DNA damage and repair has been a major focus of anticancer...Plummer R . Perspective on the pipeline of drugs being developed with...break DNA damage signaling and repair. Clin Cancer Res 2010;16...

Dorine Rossetto; Andrew W. Truman; Stephen J. Kron; and Jacques Cōté

2010-09-15T23:59:59.000Z

27

Structural investigations of platinum anticancer drugs with DNA  

SciTech Connect (OSTI)

The antitumor agent cis-diamminedichloroplatinum (II) (cis-DDP) can successfully treat testicular and ovarian cancers, presumably by binding to DNA and preventing replication. cis-DDP is less successful in treating lung and breast cancers and the trans isomer is inactive. It has been suggested that cellular recognition and repair processes may be responsible for the difference in activity between cis- and trans-DDP, the differential effectiveness against different types of cancers, as well as acquired resistance. The author reviews structural methods used to characterize several site-specific adducts. Structure-function relations that emerge may help clarify the mechanism of action. The extent of DNA bending caused by several site-specific DNA adducts formed by cis- and trans-DDP has been determined using a gel electrophoresis assay. The adducts cis-GG, cis-AG, cis-GTG, and trans-GTG were incorporated into synthetic DNA oligonucleotides of varying lengths with two bp cohesive ends. Subtle DNA distortions were amplified by polymerizing these monomers and quantitated using polyacrylamide gel electrophoresis. The three adducts cis-GG, cis-AG, and cis-GTG were all found to bend the helix in a directed fashion by about 32-35[degrees]. The trans-GTG adduct gave a degree of flexibility to the double helix, allowing bending in more than one direction. The DNA unwinding caused by the platinum binding was measured by systematically varying the interplatinum distance in a series of synthetic DNA oligonucleotides. The cis-GG and cis-AG adducts both unwind the double helix by 13[degrees]C, while the cis-GTG adduct unwinds by 23[degrees]. To determine the complete structure of platinated duplex DNA< single crystals of a platinated 12 base pair duplex oligonucletide were obtained. Despite extreme temperature and radiation sensitivity problems, a complete set of data was collected. Several different approaches to solve the structure were attempted.

Bellon, S.F.

1992-01-01T23:59:59.000Z

28

Intense photoluminescence from dried double-stranded DNA and single-walled carbon nanotube hybrid  

SciTech Connect (OSTI)

Semiconducting single-walled carbon nanotubes (SWNTs) show near-infrared photoluminescence (PL) when they are individually isolated. This was an obstacle to use photonic properties of SWNTs on a solid surface. We show that SWNTs wrapped with DNA maintain intense PL under the dry conditions. SWNTs are well isolated individually by DNA even when the DNA-SWNT hybrids are agglomerated. This finding opens up application of SWNTs to photonic devices.

Ito, M.; Kobayashi, T.; Ito, Y.; Hayashida, T.; Nii, D.; Umemura, K.; Homma, Y. [Department of Physics, Tokyo University of Science, Tokyo 162-8601 (Japan)

2014-01-27T23:59:59.000Z

29

Dyes designed for high sensitivity detection of double-stranded DNA  

DOE Patents [OSTI]

Novel fluorescent dyes are provided, characterized by having a fluorophore joined to a cationic chain. The dyes are found to provide for high enhancement upon binding to nucleic acid and have strong binding affinities to the nucleic acid, as compared to the fluorophore without the polycationic chain. The dyes find use in detection of dsDNA in gel electrophoresis and solution at substantially higher sensitivities using substantially less dye.

Glazer, Alexander N. (Orinda, CA); Benson, Scott C. (Oakland, CA)

2000-01-01T23:59:59.000Z

30

Synthesis of DNA  

DOE Patents [OSTI]

A method of synthesizing a desired double-stranded DNA of a predetermined length and of a predetermined sequence. Preselected sequence segments that will complete the desired double-stranded DNA are determined. Preselected segment sequences of DNA that will be used to complete the desired double-stranded DNA are provided. The preselected segment sequences of DNA are assembled to produce the desired double-stranded DNA.

Mariella, Jr., Raymond P. (Danville, CA)

2008-11-18T23:59:59.000Z

31

The C-Terminal RpoN Domain of sigma54 Forms an unpredictedHelix-Turn-Helix Motif Similar to domains of sigma70  

SciTech Connect (OSTI)

The ''{delta}'' subunit of prokaryotic RNA-polymerase allows gene-specific transcription initiation. Two {sigma} families have been identified, {sigma}{sup 70} and {sigma}{sup 54}, which use distinct mechanisms to initiate transcription and share no detectable sequence homology. Although the {sigma}{sup 70}-type factors have been well characterized structurally by x-ray crystallography, no high-resolution structural information is available for the {sigma}{sup 54}-type factors. Here we present the NMR derived structure of the C-terminal domain of {sigma}{sup 54} from Aquifex aeolicus. This domain (Thr323 to Gly389), which contains the highly conserved RpoN box sequence, consists of a poorly structured N-terminal tail followed by a three-helix bundle, which is surprisingly similar to domains of the {sigma}{sup 70}-type proteins. Residues of the RpoN box, which have previously been shown to be critical for DNA binding, form the second helix of an unpredicted helix-turn-helix motif. This structure's homology with other DNA binding proteins, combined with previous biochemical data, suggest how the C-terminal domain of {sigma}{sup 54} binds to DNA.

Doucleff, Michaeleen; Malak, Lawrence T.; Pelton, Jeffrey G.; Wemmer, David E.

2005-11-01T23:59:59.000Z

32

Synthetic DNA Replication Bubbles Bound and Unwound with Twofold Symmetry by a Simian Virus 40 T-Antigen Double Hexamer  

Science Journals Connector (OSTI)

ARTICLE ANIMAL VIRUSES Synthetic DNA Replication Bubbles Bound and...examined the binding of TAgDH to synthetic DNA replication bubbles. Tests of...characterized the binding and unwinding of synthetic DNA replication bubbles by the TAgDH...

Natalia V. Smelkova; James A. Borowiec

1998-11-01T23:59:59.000Z

33

Helix Atoll JV | Open Energy Information  

Open Energy Info (EERE)

Product: California-based JV developing products and financing mechanisms for small wind turbines. References: Helix & Atoll JV1 This article is a stub. You can help OpenEI by...

34

Mean First-Passage Time Calculations for the Coil-to-Helix Transition:? The Active Helix Ising Model  

Science Journals Connector (OSTI)

Mean First-Passage Time Calculations for the Coil-to-Helix Transition:? The Active Helix Ising Model† ... The kinetics and thermodynamics of the coil-to-helix transition is studied using a one-dimensional “Zimm?Bragg” Ising model. ... 4. Mean First-Passage Time for the Active-Helix Ising Model ...

Nicolae-Viorel Buchete; John E. Straub

2001-06-20T23:59:59.000Z

35

Unzipping Kinetics of Double-Stranded DNA in a Nanopore Alexis F. Sauer-Budge,1,2  

E-Print Network [OSTI]

- technological applications [11,12]. To explore strand separation in a nanopore we designed two synthetic DNA

36

Human Polynucleotide Kinase Participates in Repair of DNA Double-Strand Breaks by Nonhomologous End Joining but not Homologous Recombination  

Science Journals Connector (OSTI)

...for the repair of topoisomerase I-mediated DNA lesions. DNA Repair (Amst) 2003;2:1087-100. 42 El-Khamisy SF, Saifi GM, Weinfeld M, et al. Defective DNA single-strand break repair in spinocerebellar ataxia with axonal neuropathy-1...

Feridoun Karimi-Busheri; Aghdass Rasouli-Nia; Joan Allalunis-Turner; Michael Weinfeld

2007-07-15T23:59:59.000Z

37

DNA  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

directed directed assembly of nanoparticle linear structure for nanophotonics Baoquan Ding, aĶ’ Stefano Cabrini, bĶ’ Ronald N. Zuckermann, and Jeffrey Bokor Molecular Foundry, Lawrence Berkeley National Laboratory, 1 Cyclotron Rd., Berkeley, California 94720 Ķ‘Received 17 June 2008; accepted 22 December 2008; published 2 February 2009Ķ’ Assemblies of metal nanospheres have shown interesting properties for nanophotonics. Here the authors describe a method to use robust DNA multicrossover molecules to organize Au nanoparticles with different sizes to form well controlled linear chain structures with desired distance below 10 nm between the particles. Au particles with only one piece of DNA attached are purified individually. Three different sizes DNA-Au conjugates then hybridize with five other DNA strands to form the stiff triple crossover Ķ‘TXĶ’ motif. The linkage position

38

Induction of DNA Double-Strand Breaks and Cellular Migration Through Bystander Effects in Cells Irradiated With the Slit-Type Microplanar Beam of the Spring-8 Synchrotron  

SciTech Connect (OSTI)

Purpose: To determine whether glioma cells irradiated with a microplanar X-ray beam exert bystander effects. Methods and Materials: Microplanar beam irradiation of glioma cells in vitro was done using the SPring-8 synchrotron radiation facility. The amount of DNA double-strand breaks (dsbs) was measured by the fluorescence intensity of phosphorylated H2AX or the number of 53BP1 foci. The dose distribution in a cell population exposed to a single microplanar beam was determined by the amount of phosphorylated H2AX-positive cells. Bystander effects were determined by counting the number of 53BP1 foci in nonirradiated cells treated with conditioned medium from cultures of irradiated cells. Results: More DNA dsbs were detected in cells adjacent to an area irradiated by the single beam than in cells in distant, nonirradiated areas as a result of bystander effects caused by scattered X-rays and DNA dsbs. In support of this, more 53BP1 foci were observed in nonirradiated, conditioned medium-treated cells than in control cells (i.e., cells not treated with irradiation or conditioned medium). These results suggest that DNA dsbs were induced in nonirradiated cells by soluble factors in the culture medium. In addition, we observed cellular migration into areas irradiated with peak doses, suggesting that irradiated cells send signals that cause nonirradiated cells to migrate toward damaged cells. Conclusions: Bystander effects are produced by factors secreted as a result of slit-type microplanar X-ray beam irradiation.

Kashino, Genro [Research Reactor Institute, Kyoto University, Osaka (Japan)], E-mail: kashino@rri.kyoto-u.ac.jp; Kondoh, Takeshi [Department of Neurosurgery, Graduate School of Medicine, Kobe University, Kobe (Japan); Nariyama, Nobuteru; Umetani, Keiji; Ohigashi, Takuji [Japan Synchrotron Radiation Research Institute, Hyogo (Japan); Shinohara, Kunio [Japan Synchrotron Radiation Research Institute, Hyogo (Japan); Advanced Research Institute for Science and Engineering, Waseda University, Tokyo (Japan); Kurihara, Ai; Fukumoto, Manabu [Department of Pathology, Institute of Development, Aging and Cancer, Tohoku University, Sendai (Japan); Tanaka, Hiroki; Maruhashi, Akira; Suzuki, Minoru; Kinashi, Yuko; Liu Yong; Masunaga, Shin-ichiro; Watanabe, Masami; Ono, Koji [Research Reactor Institute, Kyoto University, Osaka (Japan)

2009-05-01T23:59:59.000Z

39

Investigating Deinococcus radiodurans RecA Protein Filament Formation on Double-Stranded DNA by a Real-Time  

E-Print Network [OSTI]

to desiccation.2 Many hypotheses have been proposed to explain the high- efficiency repair, including a ringlike condensed chromosome structure that could restrict fragment DNA diffusion,3-5 a high Mn2+ concentration: With the aid of an efficient, precise, and almost error-free DNA repair system, Deinococcus radiodurans can

Cox, Michael M.

40

Synthetic DNA Replication Bubbles Bound and Unwound with Twofold Symmetry by a Simian Virus 40 T-Antigen Double Hexamer  

Science Journals Connector (OSTI)

...yielded similar results. When the stabilities of TAgDH-DNA substrate complexes...antigen bound to the replication bubble substrates. Standard binding reactions...There was no effect of CaCl2 on the stability of the TAgDH-DNA substrate complexes...

Natalia V. Smelkova; James A. Borowiec

1998-11-01T23:59:59.000Z

Note: This page contains sample records for the topic "dna double helix" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


41

Energetics of [alpha]-helix formation in peptides and proteins  

E-Print Network [OSTI]

This thesis focuses on the energetics of !-helix formation in peptides and proteins. The [alpha]-helix is the most prevalent type of secondary structure found in proteins, and has arguably dominated our thinking about ...

Schubert, Christian Reinhold

2009-01-01T23:59:59.000Z

42

Grooving Down the Helix http://www.bnl.gov/bnlweb/pubaf/pr/PR_display.asp?prID=1038&template=Today[12/7/2009 9:39:49 AM  

E-Print Network [OSTI]

the Helix - 12/4/2009 Brookhaven Hosts ATLAS Jamboree in Anticipation of LHC Second Run - 12 move in a straight line parallel to the DNA axis or trace more complex helical paths, following

Heller, Eric

43

Mean First-Passage Time Calculations for the Coil-to-Helix Transition: The Active Helix Ising Model  

E-Print Network [OSTI]

Mean First-Passage Time Calculations for the Coil-to-Helix Transition: The Active Helix Ising Model of the coil-to-helix transition is studied using a one-dimensional "Zimm- Bragg" Ising model. The mean first-dimensional Ising model for arbitrary spin-spin coupling (J) and external field (H) where J and H are expressed

Straub, John E.

44

Specific and Efficient Binding of Xeroderma Pigmentosum Complementation Group A to Double-Strand/Single-Strand DNA Junctions with 3‘- and/or 5‘-ssDNA Branches  

Science Journals Connector (OSTI)

11.?Rademakers, S., Volker, M., Hoogstraten, D., Nigg, A. L., Mone, M. J., Van Zeeland, A. A., Hoeijmakers, J. H., Houtsmuller, A. B., and Vermeulen, W. (2003) Xeroderma pigmentosum group A protein loads as a separate factor onto DNA lesions, Mol. ... Rademakers, Suzanne; Volker, Marcel; Hoogstraten, Deborah; Nigg, Alex L.; Mone, Martijn J.; van Zeeland, Albert A.; Hoeijmakers, Jan H. J.; Houtsmuller, Adriaan B.; Vermeulen, Wim ...

Zhengguan Yang; Marina Roginskaya; Laureen C. Colis; Ashis K. Basu; Steven M. Shell; Yiyong Liu; Phillip R. Musich; Constance M. Harris; Thomas M. Harris; Yue Zou

2006-12-19T23:59:59.000Z

45

PAXX, a paralog of XRCC4 and XLF, interacts with Ku to promote DNA double-strand break repair  

E-Print Network [OSTI]

regions of PAXX1-204 and PAXXV199A/F201A were amplified from the vectors by PCR and cloned into the pcDNA3.1(-) vector (a gift from Dr. V. Bolanos-Garcia) together with GFP or FLAG tags using In-Fusion (Clontech). Vectors were sequenced by the DNA... -treated or treated for 1 hour with 300 µM phleomycin to induce large numbers of DSBs, before being harvested either at this point or after an additional 1-hour recovery period in phleomycin-free medium after three PBS washes. Cells were washed twice in ice-cold PBS...

Ochi, Takashi; Blackford, Andrew N.; Coates, Julia; Jhujh, Satpal; Mehmood, Shahid; Tamura, Naoka; Travers, Jon; Wu, Qian; Draviam, Viji M.; Robinson, Carol V.; Blundell, Tom L.; Jackson, Stephen P.

2015-01-09T23:59:59.000Z

46

Free energy landscape and characteristic forces for the initiation of DNA unzipping  

E-Print Network [OSTI]

DNA unzipping, the separation of its double helix into single strands, is crucial in modulating a host of genetic processes. Although the large-scale separation of double-stranded DNA has been studied with a variety of theoretical and experimental techniques, the minute details of the very first steps of unzipping are still unclear. Here, we use atomistic molecular dynamics (MD) simulations, coarse-grained simulations and a statistical-mechanical model to study the initiation of DNA unzipping by an external force. The calculation of the potential of mean force profiles for the initial separation of the first few terminal base pairs in a DNA oligomer reveal that forces ranging between 130 and 230 pN are needed to disrupt the first base pair, values of an order of magnitude larger than those needed to disrupt base pairs in partially unzipped DNA. The force peak has an "echo," of approximately 50 pN, at the distance that unzips the second base pair. We show that the high peak needed to initiate unzipping derives from a free energy basin that is distinct from the basins of subsequent base pairs because of entropic contributions and we highlight the microscopic origin of the peak. Our results suggest a new window of exploration for single molecule experiments.

Ahmet Mentes; Ana Maria Florescu; Elizabeth Brunk; Jeff Wereszczynski; Marc Joyeux; Ioan Andricioaei

2015-01-27T23:59:59.000Z

47

Free energy landscape and characteristic forces for the initiation of DNA unzipping  

E-Print Network [OSTI]

DNA unzipping, the separation of its double helix into single strands, is crucial in modulating a host of genetic processes. Although the large-scale separation of double-stranded DNA has been studied with a variety of theoretical and experimental techniques, the minute details of the very first steps of unzipping are still unclear. Here, we use atomistic molecular dynamics (MD) simulations, coarse-grained simulations and a statistical-mechanical model to study the initiation of DNA unzipping by an external force. The calculation of the potential of mean force profiles for the initial separation of the first few terminal base pairs in a DNA oligomer reveal that forces ranging between 130 and 230 pN are needed to disrupt the first base pair, values of an order of magnitude larger than those needed to disrupt base pairs in partially unzipped DNA. The force peak has an "echo," of approximately 50 pN, at the distance that unzips the second base pair. We show that the high peak needed to initiate unzipping derives...

Mentes, Ahmet; Brunk, Elizabeth; Wereszczynski, Jeff; Joyeux, Marc; Andricioaei, Ioan

2015-01-01T23:59:59.000Z

48

From a single double helix to paired double helices and back  

Science Journals Connector (OSTI)

...regulation in prophase. J. Cell Biol. 151, 749762. Sumara, I., Vorlaufer, E., Stukenberg, P. T., Kelm, O., Rede- mann, N., Nigg, E. A. & Peters, J. M. 2002 The dissociation of cohesin from chromosomes in prophase is regulated by...

2004-01-01T23:59:59.000Z

49

Replication protein A and g-H2AX foci assembly is triggered by cellular response to DNA double-strand breaks  

E-Print Network [OSTI]

pathway that removes the oxidized base lesions from the genomic DNA [6]. Evidence for the stimulation

50

DNA repair: Dynamic defenders against cancer and aging  

SciTech Connect (OSTI)

You probably weren't thinking about your body's cellular DNA repair systems the last time you sat on the beach in the bright sunshine. Fortunately, however, while you were subjecting your DNA to the harmful effects of ultraviolet light, your cells were busy repairing the damage. The idea that our genetic material could be damaged by the sun was not appreciated in the early days of molecular biology. When Watson and Crick discovered the structure of DNA in 1953 [1], it was assumed that DNA is fundamentally stable since it carries the blueprint of life. However, over 50 years of research have revealed that our DNA is under constant assault by sunlight, oxygen, radiation, various chemicals, and even our own cellular processes. Cleverly, evolution has provided our cells with a diverse set of tools to repair the damage that Mother Nature causes. DNA repair processes restore the normal nucleotide sequence and DNA structure of the genome after damage [2]. These responses are highly varied and exquisitely regulated. DNA repair mechanisms are traditionally characterized by the type of damage repaired. A large variety of chemical modifications can alter normal DNA bases and either lead to mutations or block transcription if not repaired, and three distinct pathways exist to remove base damage. Base excision repair (BER) corrects DNA base alterations that do not distort the overall structure of the DNA helix such as bases damaged by oxidation resulting from normal cellular metabolism. While BER removes single damaged bases, nucleotide excision repair (NER) removes short segments of nucleotides (called oligonucleotides) containing damaged bases. NER responds to any alteration that distorts the DNA helix and is the mechanism responsible for repairing bulky base damage caused by carcinogenic chemicals such as benzo [a]pyrene (found in cigarette smoke and automobile exhaust) as well as covalent linkages between adjacent pyrimidine bases resulting from the ultraviolet (UV) component of sunlight. NER can be divided into two classes based on where the repair occurs. NER occurring in DNA that is not undergoing transcription (i.e., most of the genome) is called global genome repair (GGR or GGNER), while NER taking place in the transcribed strand of active genes is called transcription-coupled repair (TCR or TC-NER). We will explore NER in more detail below. Mismatch repair (MMR) is another type of excision repair that specifically removes mispaired bases resulting from replication errors. DNA damage can also result in breaks in the DNA backbone, in one or both strands. Single-strand breaks (SSBs) are efficiently repaired by a mechanism that shares common features with the later steps in BER. Double-strand breaks (DSBs) are especially devastating since by definition there is no intact complementary strand to serve as a template for repair, and even one unrepaired DSB can be lethal [3]. In cells that have replicated their DNA prior to cell division, the missing information can be supplied by the duplicate copy, or sister chromatid, and DSBs in these cells are faithfully repaired by homologous recombination involving the exchange of strands of DNA between the two copies. However, most cells in the body are non-dividing, and in these cells the major mechanism for repairing DSBs is by non-homologous end joining (NHEJ), which as the name implies involves joining two broken DNA ends together without a requirement for homologous sequence and which therefore has a high potential for loss of genetic information.

Fuss, Jill O.; Cooper, Priscilla K.

2006-04-01T23:59:59.000Z

51

Induction and Rejoining of DNA Double Strand Breaks Assessed by H2AX Phosphorylation in Melanoma Cells Irradiated with Proton and Lithium Beams  

SciTech Connect (OSTI)

Purpose: The aim of this study was to evaluate the induction and rejoining of DNA double strand breaks (DSBs) in melanoma cells exposed to low and high linear energy transfer (LET) radiation. Methods and Materials: DSBs and survival were determined as a function of dose in melanoma cells (B16-F0) irradiated with monoenergetic proton and lithium beams and with a gamma source. Survival curves were obtained by clonogenic assay and fitted to the linear-quadratic model. DSBs were evaluated by the detection of phosphorylated histone H2AX ({gamma}H2AX) foci at 30 min and 6 h post-irradiation. Results: Survival curves showed the increasing effectiveness of radiation as a function of LET. {gamma}H2AX labeling showed an increase in the number of foci vs. dose for all the radiations evaluated. A decrease in the number of foci was found at 6 h post-irradiation for low LET radiation, revealing the repair capacity of DSBs. An increase in the size of {gamma}H2AX foci in cells irradiated with lithium beams was found, as compared with gamma and proton irradiations, which could be attributed to the clusters of DSBs induced by high LET radiation. Foci size increased at 6 h post-irradiation for lithium and proton irradiations in relation with persistent DSBs, showing a correlation with surviving fraction. Conclusions: Our results showed the response of B16-F0 cells to charged particle beams evaluated by the detection of {gamma}H2AX foci. We conclude that {gamma}H2AX foci size is an accurate parameter to correlate the rejoining of DSBs induced by different LET radiations and radiosensitivity.

Ibanez, Irene L. [Comision Nacional de Energia Atomica, San Martin (Argentina); Consejo Nacional de Investigaciones Cientificas y Tecnicas, Buenos Aires (Argentina); Bracalente, Candelaria [Comision Nacional de Energia Atomica, San Martin (Argentina); Molinari, Beatriz L. [Comision Nacional de Energia Atomica, San Martin (Argentina); Consejo Nacional de Investigaciones Cientificas y Tecnicas, Buenos Aires (Argentina); Palmieri, Monica A. [Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires (Argentina); Policastro, Lucia [Comision Nacional de Energia Atomica, San Martin (Argentina); Consejo Nacional de Investigaciones Cientificas y Tecnicas, Buenos Aires (Argentina); Kreiner, Andres J. [Comision Nacional de Energia Atomica, San Martin (Argentina); Consejo Nacional de Investigaciones Cientificas y Tecnicas, Buenos Aires (Argentina); Escuela de Ciencia y Tecnologia, Universidad Nacional de San Martin (Argentina); Burlon, Alejandro A. [Comision Nacional de Energia Atomica, San Martin (Argentina); Escuela de Ciencia y Tecnologia, Universidad Nacional de San Martin (Argentina); Valda, Alejandro [Escuela de Ciencia y Tecnologia, Universidad Nacional de San Martin (Argentina); Navalesi, Daniela [Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires (Argentina); Davidson, Jorge; Davidson, Miguel [Consejo Nacional de Investigaciones Cientificas y Tecnicas, Buenos Aires (Argentina); Vazquez, Monica; Ozafran, Mabel [Comision Nacional de Energia Atomica, San Martin (Argentina); Duran, Hebe [Comision Nacional de Energia Atomica, San Martin (Argentina); Consejo Nacional de Investigaciones Cientificas y Tecnicas, Buenos Aires (Argentina); Escuela de Ciencia y Tecnologia, Universidad Nacional de San Martin (Argentina)], E-mail: hduran@cnea.gov.ar

2009-07-15T23:59:59.000Z

52

MHK Technologies/Hydro Helix | Open Energy Information  

Open Energy Info (EERE)

Helix Helix < MHK Technologies Jump to: navigation, search << Return to the MHK database homepage Hydro Helix.jpg Technology Profile Primary Organization Hydrohelix Energies Project(s) where this technology is utilized *MHK Projects/Marenergie Technology Resource Click here Current/Tidal Technology Type Click here Axial Flow Turbine Technology Readiness Level Click here TRL 1-3: Discovery / Concept Definition / Early Stage Development & Design & Engineering Technology Description The Hydro-Helix horizontal axis turbines are stabilized by gravity and/or anchored depending on the nature of the site. They are pre-oriented to face the the tidal currents, and the profile of the rotor's blades can capture the flow and ebb tide. The rotor is activated at low speeds (10 to 15tr/mn) by the flow of the tide.

53

Three-dimensional theory of helix TWTs: Models and applications  

SciTech Connect (OSTI)

A variety of 3D configurations for modeling helix TWTs is presented. Linear field theories are described for the dispersion equations for helix TWTs. These models include a sheath helix enclosed by a conducting shell with dielectric and vane loading in the gap between the helix and the shell. The dispersion equations so obtained are solved for parameters consistent with several experiments and compared with measured results. Good agreement for the dispersion and gain is found with the linear theories and the experiments.The nonlinear analysis and simulation code is an extension of the GATOR simulation to include the vanes and dielectrics. Comparisons between GATOR and the linear theories as well as the experiments are presented, and good agreement is found.

Freund, H.P.; Zaidman, E.G. [Naval Research Lab., Washington, DC (United States)

1996-12-31T23:59:59.000Z

54

Phylogenetic distribution of DNA-binding transcription factors in bacteria and archaea  

Science Journals Connector (OSTI)

We have addressed the distribution and abundance of 75 transcription factor (TF) families in complete genomes from 90 different bacterial and archaeal species. We found that the proportion of TFs increases with genome size. The deficit of TFs in some ... Keywords: Genome size, Helix-turn-helix DNA-binding motif, Protein families, Transcription factors

Ernesto Pérez-Rueda; Julio Collado-Vides; Lorenzo Segovia

2004-12-01T23:59:59.000Z

55

Fanconi Anemia FANCG Protein in Mitigating Radiation- and Enzyme-Induced DNA Double-Strand Breaks by Homologous Recombination in Vertebrate Cells  

Science Journals Connector (OSTI)

...Protein in Mitigating Radiation- and Enzyme-Induced...Okayama 701-0192, Japan. Phone: 81-86-462-1111...exhibit increased MMC and radiation-induced chromosome...Okayama 701-0192, Japan. | Journal Article...pharmacology DNA drug effects radiation effects DNA Damage DNA...

Kazuhiko Yamamoto; Masamichi Ishiai; Nobuko Matsushita; Hiroshi Arakawa; Jane E. Lamerdin; Jean-Marie Buerstedde; Mitsune Tanimoto; Mine Harada; Larry H. Thompson; Minoru Takata

2003-08-01T23:59:59.000Z

56

DNA Assembly Method Standardization for Synthetic Biomolecular Circuits and Systems  

Science Journals Connector (OSTI)

Simply put, the DNA assembly challenge is to take a set of double-stranded DNA fragments, and physically (as well as ... to yield a single, potentially circular, assembled DNA sequence. These DNA sequence fragmen...

Nathan J. Hillson

2011-01-01T23:59:59.000Z

57

Stabilization of DNA Triple Helices Using Conjugates of Oligonucleotides and Synthetic Ligands  

Science Journals Connector (OSTI)

The possibility is discussed of stabilizing a DNA triple helix by covalent conjugation to the ... triplex-specific intercalators. As a target, a synthetic 29-mer duplex containing a natural polypurine...

A. N. Sinyakov; V. A. Ryabinin; G. N. Grimm; A. S. Boutorine

2001-03-01T23:59:59.000Z

58

Anomalous radio emission from dust in the Helix  

E-Print Network [OSTI]

A byproduct of experiments designed to map the CMB is the recent detection of a new component of foreground Galactic emission. The anomalous foreground at ~ 10--30 GHz, unexplained by traditional emission mechanisms, correlates with 100um dust emission. We report that in the Helix the emission at 31 GHz and 100um are well correlated, and exhibit similar features on sky images, which are absent in H\\beta. Upper limits on the 250 GHz continuum emission in the Helix rule out cold grains as candidates for the 31 GHz emission, and provide spectroscopic evidence for an excess at 31 GHz over bremsstrahlung. We estimate that the 100um-correlated radio emission, presumably due to dust, accounts for at least 20% of the 31 GHz emission in the Helix. This result strengthens previous tentative interpretations of diffuse ISM spectra involving a new dust emission mechanism at radio frequencies. Very small grains have not been detected in the Helix, which hampers interpreting the new component in terms of spinning dust. The observed iron depletion in the Helix favors considering the identity of this new component to be magnetic dipole emission from hot ferromagnetic grains. The reduced level of free-free continuum we report also implies an electronic temperature of Te=4600\\pm1200K for the free-free emitting material, which is significantly lower than the temperature of 9500\\pm500K inferred from collisionally-excited lines (abridged).

S. Casassus; A. C. S. Readhead; T. J. Pearson; L. -A. Nyman; M. C. Shepherd; L. Bronfman

2003-12-01T23:59:59.000Z

59

Nej1 recruits the Srs2 helicase to DNA double-strand breaks and supports repair by a single-strand annealing-like mechanism  

Science Journals Connector (OSTI)

...strain and its derivatives. Relative to input DNA, galactose-induction in the SRS2-13XMYC WT strain...performed as described in ref. 52. Input DNA was recovered before immunoprecipitation...Genetic screens and selections for cell and nuclear fusion mutants . Methods Enzymol 194 : 774...

Sidney D. Carter; Dana Vigašovį; Jiang Chen; Miroslav Chovanec; Stefan U. Åström

2009-01-01T23:59:59.000Z

60

A unified description of solvent effects in the helix-coil transition  

E-Print Network [OSTI]

We analyze the problem of the helix-coil transition in explicit solvents analytically by using spin-based models incorporating two different mechanisms of solvent action: explicit solvent action through the formation of solvent-polymer hydrogen bonds that can compete with the intrinsic intra-polymer hydrogen bonded configurations (competing interactions) and implicit solvent action, where the solvent-polymer interactions tune biopolymer configurations by changing the activity of the solvent (non-competing interactions). The overall spin Hamiltonian is comprised of three terms: the background \\emph{in vacuo} Hamiltonian of the "Generalized Model of Polypeptide Chain" type and two additive terms that account for the two above mechanisms of solvent action. We show that on this level the solvent degrees of freedom can be {\\sl explicitly} and {\\sl exactly} traced over, the ensuing effective partition function combining all the solvent effects in a unified framework. In this way we are able to address helix-coil transitions for polypeptides, proteins, and DNA, with different buffers and different external constraints. Our spin-based effective Hamiltonian is applicable for treatment of such diverse phenomena as cold denaturation, effects of osmotic pressure on the cold and warm denaturation, complicated temperature dependence of the hydrophobic effect as well as providing a conceptual base for understanding the behavior of Intrinsically Disordered Proteins and their analogues.

Artem Badasyan; Shushanik A. Tonoyan; Achille Giacometti; Rudolf Podgornik; V. Adrian Parsegian; Yevgeni Sh. Mamasakhlisov; Vladimir F. Morozov

2014-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "dna double helix" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


61

Compact Printed Quadrifilar Helix Antennas for Stratospheric Balloons Telemetry  

E-Print Network [OSTI]

Compact Printed Quadrifilar Helix Antennas for Stratospheric Balloons Telemetry L. Hanane* (1, 2 for meteorological stratospheric balloons. The PQH antenna, patented by the CNES [1], is relatively simple over a wide range of elevation angle. However the reduction in size of modern stratospheric balloon

Boyer, Edmond

62

The Sensitivity of MCF10A Breast Epithelial Cells to Alkylating Drugs is Enhanced by the Inhibition of O6-Methylguanine-DNA Methyltransferase Transcription with a Synthetic Double Strand DNA Oligonucleotide  

Science Journals Connector (OSTI)

Cytoxicity of alkylating chemotherapeutic drugs is affected by the cellular content of the enzyme O6-methylguanine-DNA methyl transferase (MGMT). Since high levels ... of the enzyme confer the efficient repair of...

Laura Mariani; Antonella Piccirilli; Lorenzo Citti…

2002-06-01T23:59:59.000Z

63

High-resolution NMR study of a synthetic DNA-RNA hybrid dodecamer containing the consensus Pribnow promoter sequence  

SciTech Connect (OSTI)

The nonsymmetrical double-helical hybrid dodecamer d(CGTTATAATGCG){center dot}r(CGCAUUAUAACG) was synthesized with solid-phase phosphoramidite methods and studied by high-resolution 2D-NMR. The imino protons were assigned by one-dimensional nuclear Overhauser methods. All the base protons and H1', H2', H2'', H3', and H4' sugar protons of the DNA strand and the base protons, H1', H2', and most of the H3'-H4' protons of the RNA strand were assigned by 2D NMR techniques. The well-resolved spectra allowed a qualitative analysis of relative proton-proton distances in both strands of the dodecamer. The chemical shifts of the hybrid duplex were compared to those of the pure DNA double helix with the same sequence. The intrastrand and cross-strand NOEs from adenine H2 to H1' resonances of neighboring base pairs exhibited characteristic patterns that were very useful for checking the spectral assignments, and their highly nonsymmetric nature reveals that the conformations of the two strands are quite different. Detailed analysis of the NOESY and COSY spectra, as well as the chemical shift data, indicate that the RNA strand assumes a normal A-type conformation (C3'-endo) whereas the DNA strand is in the general S domain but not exactly in the normal C2'-endo conformation.

Chou, S.H.; Flynn, P.; Reid, B. (Univ. of Washington, Seattle (USA))

1989-03-21T23:59:59.000Z

64

RAD-51-Dependent and -Independent Roles of a Caenorhabditis elegans BRCA2-Related Protein during DNA Double-Strand Break Repair  

Science Journals Connector (OSTI)

...previously described (5). To test pair-wise interactions in yeast cells, Gal4 DNA...four BRCs) (11, 21, 41, 49). Pair-wise alignment of the single BRC of T07E3...In addition, we thank Juliet Reid, Alison Schuldt, John Diffley, Jesper Svejstrup...

Julie S. Martin; Nicole Winkelmann; Mark I. R. Petalcorin; Michael J. McIlwraith; Simon J. Boulton

2005-04-01T23:59:59.000Z

65

Helix Wind Inc formerly ClearView Acquisitions | Open Energy Information  

Open Energy Info (EERE)

Helix Wind Inc formerly ClearView Acquisitions Helix Wind Inc formerly ClearView Acquisitions Jump to: navigation, search Name Helix Wind Inc. (formerly ClearView Acquisitions) Place San Diego, California Zip 92113 Sector Wind energy Product California-based manufacturer of small scale wind turbines. References Helix Wind Inc. (formerly ClearView Acquisitions)[1] LinkedIn Connections CrunchBase Profile No CrunchBase profile. Create one now! This article is a stub. You can help OpenEI by expanding it. Helix Wind Inc. (formerly ClearView Acquisitions) is a company located in San Diego, California . References ā†‘ "Helix Wind Inc. (formerly ClearView Acquisitions)" Retrieved from "http://en.openei.org/w/index.php?title=Helix_Wind_Inc_formerly_ClearView_Acquisitions&oldid=346471"

66

Nature Methods A primer to scaffolded DNA origami  

E-Print Network [OSTI]

Scaffold / staple layout for a tetrameric 60 helix bundle. Supplementary Protocol 1 Step-by-step scaffold production from phage DNA. Supplementary Protocol 2 Setting up folding reactions. Supplementary Protocol 3. Generated with caDNAno v0.2 Nature Methods: doi.10.1038/nmeth.1570 #12;Detailed scaffold / staple lay

Dietz, Hendrik

67

Forster resonance energy transfer in liposomes: Measurements of transmembrane helix dimerization in the native  

E-Print Network [OSTI]

FoĀØrster resonance energy transfer in liposomes: Measurements of transmembrane helix dimerization membranes. Ć? 2005 Elsevier Inc. All rights reserved. Free energy measurements of transmembrane (TM)1 helix system, however, measuring the thermodynamics of membrane protein association is a challenge. Here we

Wimley, William C.

68

New Mathematical Method Reveals Where Proteins Bind with DNA to Switch  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Mathematical Mathematical Method Reveals Where Genes Switch On or Off New Mathematical Method Reveals Where Genes Switch On or Off "Compressed sensing" determines atomic-level energy potentials with accuracy approaching experimental measurement February 22, 2012 | Tags: Biological and Environmental Research (BER), Carver, Chemistry, Life Sciences, Math & Computer Science, NISE John Hules, JAHules@lbl.gov, +1 510 486 6008 Figure 1. Helix-turn-helix (HTH) proteins are the most widely distributed family of DNA-binding proteins, occurring in all biological kingdoms. This image shows a lambda repressor HTH transcription factor (green) binding to a lambda operator DNA sequence (blue and red) of the virus bacteriophage lambda. Image: Richard Wheeler, Wikipedia

69

Helix Dipole Movement and Conformational Variability Contribute to Allosteric GDP Release in G[alpha] Subunits  

SciTech Connect (OSTI)

Heterotrimeric G proteins (Galphabetagamma) transmit signals from activated G protein-coupled receptors (GPCRs) to downstream effectors through a guanine nucleotide signaling cycle. Numerous studies indicate that the carboxy-terminal alpha5 helix of Galpha subunits participates in Galpha-receptor binding, and previous EPR studies suggest this receptor-mediated interaction induces a rotation and translation of the alpha5 helix of the Galpha subunit [Oldham, W. M., et al. (2006) Nat. Struct. Mol. Biol. 13, 772-777]. On the basis of this result, an engineered disulfide bond was designed to constrain the alpha5 helix of Galpha(i1) into its EPR-measured receptor-associated conformation through the introduction of cysteines at position 56 in the alpha1 helix and position 333 in the alpha5 helix (I56C/Q333C Galpha(i1)). A functional mimetic of the EPR-measured alpha5 helix dipole movement upon receptor association was additionally created by introduction of a positive charge at the amino terminus of this helix, D328R Galpha(i1). Both proteins exhibit a dramatically elevated level of basal nucleotide exchange. The 2.9 A resolution crystal structure of I56C/Q333C Galpha(i1) in complex with GDP-AlF(4)(-) reveals the shift of the alpha5 helix toward the guanine nucleotide binding site that is anticipated by EPR measurements. The structure of the I56C/Q333C Galpha(i1) subunit further revealed altered positions for the switch regions and throughout the Galpha(i1) subunit, accompanied by significantly elevated crystallographic temperature factors. Combined with previous evidence in the literature, the structural analysis supports the critical role of electrostatics of the alpha5 helix dipole and overall conformational variability during nucleotide release.

Preininger, Anita M.; Funk, Michael A.; Oldham, William M.; Meier, Scott M.; Johnston, Christopher A.; Adhikary, Suraj; Kimple, Adam J.; Siderovski, David P.; Hamm, Heidi E.; Iverson, Tina M.; (Vanderbilt); (UNC)

2009-06-01T23:59:59.000Z

70

Nanotechnology with DNA DNA Nanodevices  

E-Print Network [OSTI]

Nanotechnology with DNA DNA Nanodevices Friedrich C. Simmel* and Wendy U. Dittmer A DNA actuator. Introduction.............285 2. Overview: DNA Nanotechnology.......285 3. Prototypes of Nanomechanical DNA overview of DNA nanotechnology as a whole is given. The most important properties of DNA molecules

Ludwig-Maximilians-UniversitƤt, MĆ¼nchen

71

Optimized fabrication and electrical analysis of silver nanowires templated on DNA molecules  

E-Print Network [OSTI]

. In this letter, we present the base sequence design of synthetic double-stranded DNA dsDNA and fabrication of metallic silver nanowires templated on both synthetic dsDNA and bacteriophage -DNA molecules. We also as programmable interconnects in bio- electronic devices. The DNA base sequence of the synthetic unit dsDNA tile

Finkelstein, Gleb

72

Twisting and Bending Stress in DNA Minicircles  

E-Print Network [OSTI]

The interplay between bending of the molecule axis and appearance of disruptions in circular DNA molecules, with $\\sim 100$ base pairs, is addressed. Three minicircles with different radii and almost equal content of AT and GC pairs are investigated. The DNA sequences are modeled by a mesoscopic Hamiltonian which describes the essential interactions in the helix at the level of the base pair and incorporates twisting and bending degrees of freedom. Helix unwinding and bubble formation patterns are consistently computed by a path integral method that sums over a large number of molecule configurations compatible with the model potential. The path ensembles are determined, as a function of temperature, by minimizing the free energy of the system. Fluctuational openings appear along the helix to release the stress due to the bending of the molecule backbone. In agreement with the experimental findings, base pair disruptions are found with larger probability in the smallest minicircle of \\textit{66-bps} whose bending angle is $\\sim 6^{o} $. For this minicircle, a sizeable untwisting is obtained with the helical repeat showing a step-like increase at $\\tau =\\,315K$. The method can be generalized to determine the bubble probability profiles of open ends linear sequences.

Marco Zoli

2014-03-25T23:59:59.000Z

73

Nucleic Acid Library Construction Using Synthetic DNA Constructs  

Science Journals Connector (OSTI)

The synthesis of a high-diversity double-stranded DNA pool can require hundreds of milliliters of...3,10...). Large volumes are required because the maximum concentration of single-stranded synthetic DNA template...

Hani S. Zaher; Peter J. Unrau

2005-01-01T23:59:59.000Z

74

Aromatic Interactions in Proteins, DNA and Synthetic Receptors  

Science Journals Connector (OSTI)

...research-article Aromatic Interactions in Proteins, DNA and Synthetic Receptors C. A. Hunter Non-covalent interactions between...and three-dimensional structure in double-helical DNA. The - interaction model has been used to calculate the...

1993-01-01T23:59:59.000Z

75

A Golden Ruler Used to Measure DNA Structure in Solution  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

8 A Golden Ruler Used to Measure DNA Structure in Solution The first crystal structures of DNA double helices appeared in 1979 and directly confirmed the atomic model of...

76

GATOR: A 3-D time-dependent simulation code for helix TWTs  

SciTech Connect (OSTI)

A 3D nonlinear analysis of helix TWTs is presented. The analysis and simulation code is based upon a spectral decomposition using the vacuum sheath helix modes. The field equations are integrated on a grid and advanced in time using a MacCormack predictor-corrector scheme, and the electron orbit equations are integrated using a fourth order Runge-Kutta algorithm. Charge is accumulated on the grid and the field is interpolated to the particle location by a linear map. The effect of dielectric liners on the vacuum sheath helix dispersion is included in the analysis. Several numerical cases are considered. Simulation of the injection of a DC beam and a signal at a single frequency is compared with a linear field theory of the helix TWT interaction, and good agreement is found.

Zaidman, E.G.; Freund, H.P. [Naval Research Lab., Washington, DC (United States)

1996-12-31T23:59:59.000Z

77

Recognition of Duplex RNA by Helix-Threading Peptides Barry D. Gooch and Peter A. Beal*  

E-Print Network [OSTI]

Recognition of Duplex RNA by Helix-Threading Peptides Barry D. Gooch and Peter A. Beal 84112-0850 Received April 15, 2004; E-mail: beal@chem.utah.edu Abstract: Many important biological

Beal, Peter A.

78

Knots in the Helix Nebula found in H2  

E-Print Network [OSTI]

We present a deep and wide field-of-view (4'x 7') image of the planetary nebula (PN) NGC 7293 (the Helix Nebula) in the 2.12 micron H2 v=1-0 S(1) line. The excellent seeing (0.4'') at the Subaru Telescope, allows the details of cometary knots to be examined. The knots are found at distances of 2.2'-6.4' from the central star (CS). At the inner edge and in the inner ring (up to 4.5' fromthe CS), the knot often show a `tadpole' shape, an elliptical head with a bright crescent inside and a long tail opposite to the CS. In detail, there are variations in the tadpole shapes, such as narrowing tails, widening tails, meandering tails, or multi-peaks within a tail. In the outer ring (4.5'-6.4' from the CS), the shapes are more fractured, and the tails do not collimate into a single direction. The transition in knot morphology from the inner edge to the outer ring is clearly seen. The number density of knots governs the H2 surface brightness in the inner ring: H2 exists only within the knots. Possible mechanisms which...

Matsuura, M; McHunu, B M; Tanaka, I; Wright, N J; Smith, M D; Zijlstra, A A; Viti, S; Wesson, R

2009-01-01T23:59:59.000Z

79

Sustainability Double Degree Double Degree Info  

E-Print Network [OSTI]

Sustainability Double Degree Double Degree Info: Ā· 36 credits in B for graduation. Sustainability Core: Take each course below for a total of 17 -20 credits. Term/Grade Course _____ ____ *NR 350 (4) Sustainable

GrĆ¼nwald, Niklaus J.

80

DNA Extraction  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

DNA Extraction DNA Extraction Being able to extract deoxyribonucleic acid (DNA) is important for a number of reasons. By studying DNA, scientists can identify genetic disorders or diseases, and they can also possibly find cures for them by manipulating or experimenting with this DNA. At the Laboratory, researchers have studied DNA to detect biothreat agents in environmental and forensic samples. Scientists also are studying how human DNA may be destroyed by certain types of electromagnetic waves at certain frequencies. Classroom Activity: This activity is about the extraction of DNA from strawberries. Strawberries are a great fruit to use for this lesson because each student can work on his or her own. Strawberries are recommended because they yield more DNA than any other fruit. Strawberries are octoploid, which means that they have eight copies of each

Note: This page contains sample records for the topic "dna double helix" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


81

Translocation of molecules into cells by pH-dependent insertion of a transmembrane helix  

E-Print Network [OSTI]

control, or cell regulation. drug delivery peptide nucleic acid delivery tumors membrane transport helix cellular processes, such as signal and energy transduction, active transport, ion flow, cellĀ­cell inter. We used the membrane- impermeable dithionite ion (S2O4 2), which can chemically modify the 4-chloro-7

82

An inverse correlation between loop length and stability in a four-helix-bundle protein  

E-Print Network [OSTI]

their intrinsic func- tionality upon their new environmental context: the calcium-binding activity of a loop has the molten globule behavior of apo- - lactalbumin in the absence of calcium [18]. Conversely, loops have beenAn inverse correlation between loop length and stability in a four-helix-bundle protein Athena D

Mochrie, Simon

83

Small-molecule mimics of an a-helix for efficient transport of proteins into cells  

E-Print Network [OSTI]

Ā­57) of HIV-1 Tat, the third helix of antennapedia homeodomain (penetratin)3,4 and designed variants was galvanized by a report6 on the ability of HIV-1 Tat fusion proteins to deliver b-galactosi- dase to multiple

Cai, Long

84

The DNA Damage Response Signaling Cascade Regulates Proliferation of the Phytopathogenic Fungus Ustilago maydis in Planta  

Science Journals Connector (OSTI)

...and Sanchez, 2004) in a broad range of eukaryotes, including U. maydis...double-strand breaks in DNA; and ionizing radiation (IR), which generates double-strand...were irradiated at the indicated dose after being spotted. The spots...

Carmen de Sena-TomƔs; Alfonso FernƔndez-Ɓlvarez; William K. Holloman; JosƩ PƩrez-Martƭn

2011-04-08T23:59:59.000Z

85

DNA Duplication Revealed in New Beginnings | Department of Energy  

Broader source: Energy.gov (indexed) [DOE]

DNA Duplication Revealed in New Beginnings DNA Duplication Revealed in New Beginnings DNA Duplication Revealed in New Beginnings April 3, 2012 - 9:36am Addthis The DNA replication origin recognition complex (ORC) is a six-protein machine with a slightly twisted half-ring structure (yellow). ORC is proposed to wrap around and bend approximately 70 base pairs of double stranded DNA (red and blue). When a replication initiator Cdc6 (green) joins ORC, the partial ring is now complete and ready to load another protein onto the DNA. This last protein (not shown) is the enzyme that unwinds the double stranded DNA so each strand can be replicated. | Illustration courtesy of Brookhaven Lab. The DNA replication origin recognition complex (ORC) is a six-protein machine with a slightly twisted half-ring structure (yellow). ORC is

86

Casa de Oro-Mount Helix, California: Energy Resources | Open Energy  

Open Energy Info (EERE)

Oro-Mount Helix, California: Energy Resources Oro-Mount Helix, California: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 32.7622263Ā°, -116.9793554Ā° Loading map... {"minzoom":false,"mappingservice":"googlemaps3","type":"ROADMAP","zoom":14,"types":["ROADMAP","SATELLITE","HYBRID","TERRAIN"],"geoservice":"google","maxzoom":false,"width":"600px","height":"350px","centre":false,"title":"","label":"","icon":"","visitedicon":"","lines":[],"polygons":[],"circles":[],"rectangles":[],"copycoords":false,"static":false,"wmsoverlay":"","layers":[],"controls":["pan","zoom","type","scale","streetview"],"zoomstyle":"DEFAULT","typestyle":"DEFAULT","autoinfowindows":false,"kml":[],"gkml":[],"fusiontables":[],"resizable":false,"tilt":0,"kmlrezoom":false,"poi":true,"imageoverlays":[],"markercluster":false,"searchmarkers":"","locations":[{"text":"","title":"","link":null,"lat":32.7622263,"lon":-116.9793554,"alt":0,"address":"","icon":"","group":"","inlineLabel":"","visitedicon":""}]}

87

Helix Nebula and CERN: A Symbiotic approach to exploiting commercial clouds  

E-Print Network [OSTI]

The recent paradigm shift toward cloud computing in IT, and general interest in "Big Data" in particular, have demonstrated that the computing requirements of HEP are no longer globally unique. Indeed, the CERN IT department and LHC experiments have already made significant R&D investments in delivering and exploiting cloud computing resources. While a number of technical evaluations of interesting commercial offerings from global IT enterprises have been performed by various physics labs, further technical, security, sociological, and legal issues need to be address before their large-scale adoption by the research community can be envisaged. Helix Nebula - the Science Cloud is an initiative that explores these questions by joining the forces of three European research institutes (CERN, ESA and EMBL) with leading European commercial IT enterprises. The goals of Helix Nebula are to establish a cloud platform federating multiple commercial cloud providers, along with new business models, which can sustain ...

Barreiro Megino, Fernando Harald; Kucharczyk, Katarzyna; Medrano Llamas, Ramón; van der Ster, Daniel

2013-01-01T23:59:59.000Z

88

Testing the helix model for protein folding on four simple proteins  

E-Print Network [OSTI]

We test a simplified, local version of the helix model on two synthetic and two natural proteins, to study its efficiency in predicting the native secondary structure. The results we obtain are very good for the synthetic sequences, poorer for the two natural ones. This suggests that non-local terms play a fundamental role in determining the secondary structure, even if in some cases local terms alone may be sufficient.

Pierpaolo Bruscolini

1997-08-19T23:59:59.000Z

89

The earliest events in protein folding: Helix dynamics in proteins and model peptides  

SciTech Connect (OSTI)

The earliest events in protein folding are critically important in determining the folding pathway, but have proved difficult to study by conventional approaches. We have developed new rapid initiation methods and structure-specific probes to interrogate the earliest events of protein folding. Our focus is the pathways. Folding or unfolding reactions are initiated on a fast timescale (10 ns) using a laser induced temperature jump (15 C) and probed with time-resolved infrared spectroscopy. We obtained the kinetics of the helix-coil transition for a model 21-residue peptide. The observed rate constant k{sub obs} = k{sub f} + k{sub u} for reversible kinetics; from the observed rate (6 x 10{sup 6} s{sup -1}) and the equilibrium constant favoring folding of 7.5 at 27 C, we calculate a folding lifetime of 180 ns and an unfolding lifetime of 1.4 {mu}s. The {open_quotes}molten globule{close_quotes} form of apomyoglobin (horse, pH*3, 0.15M NaCl) shows similar kinetics for helix that is unconstrained by tertiary structure (helix with an unusually low Amide I frequency, near 1633 cm{sup -1}). In {open_quotes}native{close_quotes} apomyoglobin (horse, pH*5.3, 10 mM NaCl) two very different rates (45 ns and 70 {mu}s) are observed and we infer that a third occurs on a timescales inaccessible to our experiment (> 1 ms). We suggest that the slower processes are due to helix formation that is rate-limited by the formation of tertiary structure.

Dyer, R.B.; Williams, S.; Woodruff, W.H. [Los Alamos National Lab., NM (United States)] [and others

1996-12-31T23:59:59.000Z

90

Mass spectrometric characterization of sequence-specific complexes of DNA and transcription factor PU.1 DNA binding domain  

SciTech Connect (OSTI)

Electrospray ionization mass spectrometry (ESI-MS) has been used to study the noncovalent interaction of the 13.5-kDa DNA binding domain of PU.1 (PU.1-DBD) with specific double-stranded DNA (dsDNA) target molecules. Mixtures of PU.1-DBD protein and wildtype target DNA sequence yielded ESI-MS spectra showing only protein-dsDNA complex ions of 1:1 stoichiometry and free dsDNA. When PU.1-DBD protein, wild type target DNA, and a mutant target DNA lacking the consensus sequence were mixed, only the 1:1 complex with the wild-type DNA was observed, consistent with gel electrophoresis mobility shift assay results, demonstrating the observation of sequence-specific protein-dsDNA complexes using ESI-MS. 22 refs., 5 figs., 1 tab.

Cheng, Xueheng; Harms, A.C.; Bruce, J.E. [Pacific Northwest National Lab., Richland, WA (United States)] [and others] [Pacific Northwest National Lab., Richland, WA (United States); and others

1996-07-15T23:59:59.000Z

91

Electrons in Dry DNA from Density Functional Calculations  

E-Print Network [OSTI]

The electronic structure of an infinite poly-guanine - poly-cytosine DNA molecule in its dry A-helix structure is studied by means of density-functional calculations. An extensive study of 30 nucleic base pairs is performed to validate the method. The electronic energy bands of DNA close to the Fermi level are then analyzed in order to clarify the electron transport properties in this particularly simple DNA realization, probably the best suited candidate for conduction. The energy scale found for the relevant band widths, as compared with the energy fluctuations of vibrational or genetic-sequence origin, makes highly implausible the coherent transport of electrons in this system. The possibility of diffusive transport with sub-nanometer mean free paths is, however, still open. Information for model Hamiltonians for conduction is provided.

E. Artacho; M. Machado; D. Sanchez-Portal; P. Ordejon; J. M. Soler

2002-09-24T23:59:59.000Z

92

Neutron scattering study of the hydration hull of DNA by H/sub 2/O/D/sub 2/O-exchange  

SciTech Connect (OSTI)

Films of highly oriented DNA (A-, B-, and C-conformation) were investigated by neutron scattering. Diffuse sheets corresponding to a one-dimensionally periodic ''water-DNA'' structure are identified close to the intersection of the helix direction with the structure factor maximum of bulk water. The correlation length in this structure is strongly influenced by its commensurability with the axial translation H per nucleotide. The fiber or lateral chain interaction is enhanced in the commensurate case. 10 refs., 3 figs.

Grimm, H.; Stiller, H.; Majkrzak, C.F.; Rupprecht, A.

1988-01-01T23:59:59.000Z

93

A Three-Helix Junction Is the Interface between Two Functional Domains of Prohead RNA in ?29 DNA Packaging  

Science Journals Connector (OSTI)

...Junction Is the Interface between Two...reaction by measuring the production...reference to a standard curve. To determine...for three-dimensional reconstruction...bars represent standard deviations...cryoEM three-dimensional (3D) image...junction is the interface between two...

Wei Zhao; Mitul Saha; Ailong Ke; Marc C. Morais; Paul J. Jardine; Shelley Grimes

2012-08-15T23:59:59.000Z

94

Determination of nucleotide sequences in DNA  

Science Journals Connector (OSTI)

...nu-cleotides long, which codes for ten genes. The most unexpected finding from this work was the presence of 3' 5' 5' 3' sExo l 5' W3e_3 5' Fig. 5. Degradation of double-stranded DNA with exonuclease III. Restriction Galactosidase enzyme site...

F Sanger

1981-12-11T23:59:59.000Z

95

Observation of acoustic umklapp-phonons in water-stabilized DNA by neutron scattering  

SciTech Connect (OSTI)

We have investigated static and dynamic properties of wet-spun DNA fibers in their C, A, and B conformations using the thermal neutron scattering. Over-damped and underdamped longitudinal acoustic umklapp phonons are observed in the vicinity of a hydration-dependent two-dimensional sheet of diffuse scattering perpendicular to the helix axis. A quantitative analysis of this mode is presented for the B-DNA sample in terms of a harmonic one-dimensional liquid, i.e., taking into account all orders of fluctuations. The resulting effective dispersion does not extrapolate to zero frequency at the position of the sheet.

Grimm, H.; Stiller, H.; Majkrzak, C.F.; Rupprecht, A.; Dahlborg, U.

1987-10-12T23:59:59.000Z

96

Nucleotide-dependent displacement and dynamics of the ?-1 helix in kinesin revealed by site-directed spin labeling EPR  

SciTech Connect (OSTI)

Highlights: •Dipolar EPR detects the distance between the spin-labeled kinesin ?-1 and ?-2 helices. •The distance has at least two populations: 1.5 nm (in crystal form: 20%) and >2.5 nm. •The short distance conformer was populated 40% in the apo state with microtubules. •ATP analog or ADP binding caused the 1.5 nm distance to be less populated (?20%). •The ?-1 helix moves closer to the neck-linker (away from ?-2) to facilitate docking. -- Abstract: In kinesin X-ray crystal structures, the N-terminal region of the ?-1 helix is adjacent to the adenine ring of the bound nucleotide, while the C-terminal region of the helix is near the neck-linker (NL). Here, we monitor the displacement of the ?-1 helix within a kinesin monomer bound to microtubules (MTs) in the presence or absence of nucleotides using site-directed spin labeling EPR. Kinesin was doubly spin-labeled at the ?-1 and ?-2 helices, and the resulting EPR spectrum showed dipolar broadening. The inter-helix distance distribution showed that 20% of the spins have a peak characteristic of 1.4–1.7 nm separation, which is similar to what is predicted from the X-ray crystal structure, albeit 80% were beyond the sensitivity limit (>2.5 nm) of the method. Upon MT binding, the fraction of kinesin exhibiting an inter-helix distance of 1.4–1.7 nm in the presence of AMPPNP (a non-hydrolysable ATP analog) and ADP was 20% and 25%, respectively. In the absence of nucleotide, this fraction increased to 40–50%. These nucleotide-induced changes in the fraction of kinesin undergoing displacement of the ?-1 helix were found to be related to the fraction in which the NL undocked from the motor core. It is therefore suggested that a shift in the ?-1 helix conformational equilibrium occurs upon nucleotide binding and release, and this shift controls NL docking onto the motor core.

Yasuda, Satoshi; Yanagi, Takanori [Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043 (Japan)] [Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043 (Japan); Yamada, Masafumi D. [Division of Bioengineering, Graduate School of Engineering, Soka University, Hachioji, Tokyo 192-8577 (Japan)] [Division of Bioengineering, Graduate School of Engineering, Soka University, Hachioji, Tokyo 192-8577 (Japan); Ueki, Shoji [Kagawa School of Pharmaceutical Sciences, Tokushima Bunri University, Shido 1314-1, Samuki, Kagawa 769-2193 (Japan)] [Kagawa School of Pharmaceutical Sciences, Tokushima Bunri University, Shido 1314-1, Samuki, Kagawa 769-2193 (Japan); Maruta, Shinsaku [Division of Bioengineering, Graduate School of Engineering, Soka University, Hachioji, Tokyo 192-8577 (Japan)] [Division of Bioengineering, Graduate School of Engineering, Soka University, Hachioji, Tokyo 192-8577 (Japan); Inoue, Akio [Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043 (Japan)] [Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043 (Japan); Arata, Toshiaki, E-mail: arata@bio.sci.osaka-u.ac.jp [Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043 (Japan)] [Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043 (Japan)

2014-01-17T23:59:59.000Z

97

DNA Activity  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

DNA Activity DNA Activity Name: Sara Location: N/A Country: N/A Date: N/A Question: Is DNA an anion or a cation? I thought since it was negatively charged it was an anion but mt teacher in class today said it was a cation because negatively charged molecules logically migrate to the positively charged plate of the cathode, ie molecules that migrate towards a cathode are cations. Where is the error in my logic or there error in my logic? Replies: DNA is negatively charged due to the phosphate ions present in the ribose-phosphate backbone. It moves towards the positive pole during electrophoresis. The definition kation/anion is confusing because: 1. a cation moves to the cathode 2. the cathode is negative, thus 3. a cation is positive DNA is an anion. The confusion is that a cathode is negative, but a cation is positively charged. For that reason these terms are not generally used in this context.

98

Neutrinoless Double $?$-Decay  

E-Print Network [OSTI]

The neutrinoless double $\\beta$-decay is reviewed. Model independent evidence in favor of neutrino masses and mixing is briefly summarized. The data of the recent experiments on the search for $0\

S. M. Bilenky

2004-03-23T23:59:59.000Z

99

Double Beta Decay  

E-Print Network [OSTI]

The motivation, present status, and future plans of the search for the neutrinoless double beta decay are reviewed. It is argued that, motivated by the recent observations of neutrino oscillations, there is a reasonable hope that neutrinoless double beta decay corresponding to the neutrino mass scale suggested by oscillations, of about 50 meV, actually exists. The challenges to achieve the sensitivity corresponding to this mass scale, and plans to overcome them, are described.

Steven R. Elliott; Petr Vogel

2002-02-27T23:59:59.000Z

100

The Fusion Core Complex of the Peste des Petits Ruminants Virus Is a Six-Helix Bundle Assembly  

E-Print Network [OSTI]

The Fusion Core Complex of the Peste des Petits Ruminants Virus Is a Six-Helix Bundle Assembly (PPRV) fusion protein (F) to obtain insights into the mechanism by which these repeats influence PPRV-mediated cell fusion. Both HR1 and HR2 inhibit PPRV-mediated syncytia formation in Vero cells in vitro. Of these

Srinivasan, N.

Note: This page contains sample records for the topic "dna double helix" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


101

Low Dose Radiation Research Program: Assessing Biological Function of DNA  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Assessing Biological Function of DNA Damage Response Genes Assessing Biological Function of DNA Damage Response Genes Larry H. Thompson Lawrence Livermore National Laboratory Why This Project To understand the relative importance of individual DNA repair and DNA-damage response pathways to the recovery of mammalian cells after exposure to low doses of ionizing radiation (IR). This understanding may lead to better ways of setting limits on human exposure to IR. In spite of the discovery of many mammalian DNA repair genes, our current knowledge of how many of these genes contribute to cellular recovery from IR exposure is quite limited. Project Goals Measure cellular responses at doses in the 5-100 cGy range, which generally cause changes too small to detect in normal, repair-proficient cells Focus on DNA double-strand breaks (DSBs) and DNA oxidative base

102

Double-Skin Faēades  

Science Journals Connector (OSTI)

The double-skin faēade is a system that consists of two building skins separated by a ventilated cavity (Fig. 9.1). The main aim of the cavity is to vary the physical properties of the faēade throughout the ye...

2009-01-01T23:59:59.000Z

103

DOUBLE MAJORS Imaging Science + ...  

E-Print Network [OSTI]

DOUBLE MAJORS Imaging Science + ... Applied Mathematics Biomedical Sciences Computer Science Undergraduate Research Internships and Cooperative Education (Co-op) (optional) Study Abroad WHY IMAGING SCIENCE Science: BS, MS, PhD Color Science: MS, PhD BS + MS/PhD Combos HUMAN VISION BIO- MEDICAL ASTRO- PHYSICS

Zanibbi, Richard

104

Chemically accurate coarse graining of double-stranded DNA  

Science Journals Connector (OSTI)

...Phys Chem B 112, 9135 ZZQQhy9145. 8. Chen, A. A & Pappu, R. V. (2007) Parameters of monovalent ions in the amber-99...Chem B 111, 11884 ZZQQhy11887. 14 9. Chen, A. A & Pappu, R. V. (2007) Quantitative characterization of ion pairing...

Alexey Savelyev; Garegin A. Papoian

2010-01-01T23:59:59.000Z

105

WHY SEARCH FOR DOUBLE BETA DECAY?  

E-Print Network [OSTI]

the search for neutrinoless double beta decay may prove verySearching for neutrinoless double beta decay is the onlysensitivity of neutrinoless double beta decay. The potential

Kayser, B.

2010-01-01T23:59:59.000Z

106

Double Flash | Open Energy Information  

Open Energy Info (EERE)

Flash Jump to: navigation, search Retrieved from "http:en.openei.orgwindex.php?titleDoubleFlash&oldid599606"...

107

The development of a model of Alpha helix formation for transmembrane peptides  

E-Print Network [OSTI]

sequence was ordered from integrated DNA Technologtes, Inc. Transformations took place using electroporation and heat shock methods into a competent E. coif cell line. Transformed cells were plated on Luria broth (LB) agar plates containing ampicillin...

Funk, Geoffrey Alexander

2013-02-22T23:59:59.000Z

108

Northwestern University Recombinant DNA  

E-Print Network [OSTI]

natural or synthetic DNA segments to DNA molecules that can replicate in a living cell Ā· molecules that result from the replication of those described above Synthetic DNA segments which are likely to yield) are considered as equivalent to their natural DNA counterpart. If the synthetic DNA segment is not expressed

Shull, Kenneth R.

109

Domain walls and double bubbles  

Science Journals Connector (OSTI)

...5, which are never double bubbles, that is, none is the global...transverse cylinder, torus bubble, inner tube and double hydrant...transverse cylinder and the stability of the cylinder cross. The...three-dimensional double bubbles and show that the method can...

2009-01-01T23:59:59.000Z

110

Remarkable optics of short-pitch deformed helix ferroelectric liquid crystals: symmetries, exceptional points and polarization-resolved angular patterns  

E-Print Network [OSTI]

In order to explore electric-field-induced transformations of polarization singularities in the polarization-resolved angular (conoscopic) patterns emerging after deformed helix ferroelectric liquid crystal (DHFLC) cells with subwavelength helix pitch, we combine the transfer matrix formalism with the results for the effective dielectric tensor of biaxial FLCs evaluated using an improved technique of averaging over distorted helical structures. Within the framework of the transfer matrix method, we deduce a number of symmetry relations and show that the symmetry axis of L lines (curves of linear polarization) is directed along the major in-plane optical axis which rotates under the action of the electric field. When the angle between this axis and the polarization plane of incident linearly polarized light is above its critical value, the C points (points of circular polarization) appear in the form of symmetrically arranged chains of densely packed star-monstar pairs. We also emphasize the role of phase sing...

Kiselev, Alexei D

2014-01-01T23:59:59.000Z

111

Chemical mapping of DNA and counter-ion content inside phage by energy-filtered TEM  

Science Journals Connector (OSTI)

Double-stranded DNA in many bacterial viruses (phage) is strongly confined, which results in internal genome pressures of tens of atmospheres. This pressure is strongly dependent on local ion concentration and di...

Pernilla Nevsten; Alex Evilevitch; Reine Wallenberg

2012-03-01T23:59:59.000Z

112

Triple Helix innovation in China’s dye-sensitized solar cell industry: hybrid methods with semantic TRIZ and technology roadmapping  

Science Journals Connector (OSTI)

In recent years, the Triple Helix model has identified feasible approaches to measuring relations among universities, industries, and governments. Results have been extended to different databases, regions, and p...

Yi Zhang; Xiao Zhou; Alan L. Porter; Jose M. Vicente Gomila; An Yan

2014-04-01T23:59:59.000Z

113

576 IEEE TRANSACTIONS ON PLASMA SCIENCE, VOL. 28, NO. 3, JUNE 2000 A Comparison of L-Band Helix TWT Experiments  

E-Print Network [OSTI]

576 IEEE TRANSACTIONS ON PLASMA SCIENCE, VOL. 28, NO. 3, JUNE 2000 A Comparison of L-Band Helix TWT. Publisher Item Identifier S 0093-3813(00)05375-3. With the projected growth of the telecommunications market

Hassam, Adil

114

Field-induced reorientation of the spin helix in MnSi near T{sub c}  

SciTech Connect (OSTI)

The chiral spin fluctuations and the spiral structure of the single crystal MnSi has been studied by small angle polarized neutron scattering near T{sub c}=29 K under applied field. A weak magnetic field applied along one of the <111> axes produces a single domain sample with the helix wave vector along the field. The 90 degree sign reorientation of the spin spiral from the [111] axis to [110] axis is observed in the field range from 130 to 180 mT in the close vicinity to T{sub c}. Further increase of the field above 180 mT restores the original orientation of the helix and leads to the induced ferromagnetic state at 350 mT. This observation clarifies the nature of the structural spin instability found in the H-T phase diagram of MnSi by other techniques. We explain this phenomenon as a result of competition of cubic anisotropy and the spin-wave Bose condensation provoked by the field perpendicular to the helix axis.

Grigoriev, S. V.; Maleyev, S. V.; Okorokov, A. I.; Chetverikov, Yu. O. [Petersburg Nuclear Physics Institute, Gatchina, St. Petersburg 188300 (Russian Federation); Eckerlebe, H. [GKSS Forschungszentrum, 21502 Geesthacht (Germany)

2006-06-01T23:59:59.000Z

115

Persistent DNA damage foci, cellular senescence and low dose radiation  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Persistent DNA damage foci, cellular senescence and low dose radiation Persistent DNA damage foci, cellular senescence and low dose radiation Denise Munoz 1 , Albert Davalos 1 , Francis Rodier 1 , Misako Kawahara 1 , Judith Campisi 1,2 and Steven Yannone 1,3 1 Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Mailstop 84-171, Berkeley CA 94720; 2 Buck Institute for Age Research, 8001 Redwood Boulevard, Novato CA 94945; 3 Corresponding author Ionizing radiation (IR) induced DNA double-strand breaks (DSBs) are cytologically detectable as large nuclear foci that contain phosphorylated histone H2AX (Ī³H2AX), the adaptor protein 53BP1, and several other proteins that participate in the sensing and processing of DNA damage (DNA damage foci). In normal human cells, moderately high IR (0.5-1 Gy) doses cause the rapid appearance of these foci (acute DNA damage foci), which gradually disappear

116

Live Cell Imaging and in situ analysis of cellular responses to DNA doublestrand  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Live Cell Imaging and in situ analysis of cellular responses to DNA double- Live Cell Imaging and in situ analysis of cellular responses to DNA double- strand breaks in mammalian cells. David J. Chen Division of Molecular Radiation Biology, Department of Radiation Oncology, University of Texas Southwestern Medical Center, Dallas TX 75390 The integrity of the human genome is constantly threatened by internal as well as external factors with the propensity to cause DNA damage. Of the various types of DNA damage that can occur within the mammalian cell nucleus, the DNA double strand break (DSB) is perhaps the most dangerous. Estimates put the number of endogenous DSBs anywhere between 10-100 per nucleus per day. A direct link between DSBs and cancer has been surmised by researchers based upon the fact that many cancer-predisposition

117

Use of recombinant DNA (rDNA) and Biohazardous Materials  

E-Print Network [OSTI]

constructed outside living cells by joining natural or synthetic DNA segments to DNA molecules that can

Danforth, Bryan Nicholas

118

Asiakastyytyväisyystutkimus - DNA Kauppa Lappeenranta.  

E-Print Network [OSTI]

??Opinnäytetyön aiheena oli DNA Kauppa Lappeenrannan asiakastyytyväisyyden tutkiminen. Tutkimuksen ensisijaisena tavoitteena oli selvittää, kuinka tyytyväisiä asiakkaat olivat olleet DNA Kauppa Lappeenrannan ja asiakkaan väliseen kontaktipintaan… (more)

Pitkänen, Jesse

2013-01-01T23:59:59.000Z

119

Dynamic Motion of Helix A in the Amino-terminal Domain of Calmodulin is Stabilized Upon Calcium Activation  

SciTech Connect (OSTI)

Calcium-dependent changes in the internal dynamics and average structures of the opposing globular domains of calmodulin (CaM), as well as their relative spatial arrangement, contribute to the productive association between CaM and a range of different target proteins to affect their functional activation. To identify dynamic structural changes involving individual ?-helical elements and their modulation by calcium activation, we have used site-directed mutagenesis to engineer a tetracysteine binding motif within helix A near the amino-terminus of calmodulin (CaM), permitting the selective and rigid attachment of the fluorescent probe 4?,5?-bis(1,3,2-dithioarsolan-2-yl) fluorescein (FlAsH) with full retention of function. The rigid tetracoordinate linkage of FlAsH to CaM, in conjunction with frequency-domain fluorescence anisotropy measurements, allows assessment of dynamic changes associated with calcium activation without interference from independent probe motion. Taking advantage of the large fluorescent enhancement associated with FlAsH binding to CaM, rates of FlAsH binding to apo- and calcium-activated CaM were 2500 ? 10 M-1 sec-1 and 187 ? 2 M-1 sec-1, respectively. There is no difference in the solvent accessibility of the bound FlAsH irrespective of calcium binding to CaM. Thus, given that FlAsH selectively labels disordered structures, the large difference in rates of FlAsH binding indicates that calcium binding stabilizes helix A. Frequency-domain anisotropy measurements of bound FlAsH indicate that prior to calcium activation, helix A undergoes large amplitude nanosecond motions. Following calcium activation, helix A becomes immobile, and structurally coupled to the overall rotation of CaM. We discuss these results in the context of a model that suggests stabilization of helix A relative to other domain elements in the CaM structure is critical to defining high-affinity binding clefts, and in promoting specific and ordered biding of the opposing lobes of CaM to target proteins.

Chen, Baowei; Mayer, M ULJANA.; Markillie, Lye MENG.; Stenoien, David L.; Squier, Thomas C.

2005-01-01T23:59:59.000Z

120

Dynamic Motion of Helix A in the Amino-terminal Domain of Calmodulin is Stabilized Upon Calcium Activation  

SciTech Connect (OSTI)

Calcium-dependent changes in the internal dynamics and average structures of the opposing globular domains of calmodulin (CaM), as well as their relative spatial arrangement, contribute to the productive association between CaM and a range of different target proteins to affect their functional activation. To identify dynamic structural changes involving individual ?-helical elements and their modulation by calcium activation, we have used site-directed mutagenesis to engineer a tetracysteine binding motif within helix A near the amino-terminus of calmodulin (CaM), permitting the selective and rigid attachment of the fluorescent probe 4?,5?-bis(1,3,2-dithioarsolan-2-yl) fluorescein (FlAsH) with full retention of function. The rigid tetracoordinate linkage of FlAsH to CaM, in conjunction with frequency-domain fluorescence anisotropy measurements, allows assessment of dynamic changes associated with calcium activation without interference from independent probe motion. Taking advantage of the large fluorescent enhancement associated with FlAsH binding to CaM, rates of FlAsH binding to apo- and calcium-activated CaM were 2500 ? 10 M-1 sec-1 and 187 ? 2 M-1 sec-1, respectively. There is no difference in the solvent accessibility of the bound FlAsH irrespective of calcium binding to CaM. Thus, given that FlAsH selectively labels disordered structures, the large difference in rates of FlAsH binding indicates that calcium binding stabilizes helix A. Frequency-domain anisotropy measurements of bound FlAsH indicate that prior to calcium activation, helix A undergoes large amplitude nanosecond motions. Following calcium activation, helix A becomes immobile, and structurally coupled to the overall rotation of CaM. We discuss these results in the context of a model that suggests stabilization of helix A relative to other domain elements in the CaM structure is critical to defining high-affinity binding clefts, and in promoting specific and ordered biding of the opposing lobes of CaM to target proteins.

Chen, Baowei; Mayer, M ULJANA.; Markillie, Lye MENG.; Stenoien, David L.; Squier, Thomas C.

2005-02-01T23:59:59.000Z

Note: This page contains sample records for the topic "dna double helix" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


121

A mammalian origin of bidirectional DNA replication within the Chinese hamster RPS14 locus.  

Science Journals Connector (OSTI)

...hybridization with a different nick translated [32P]DNA probe...labeled with [32P]dATP by nick translation. Identification...50). This approach has the merit of not requiring synchronized...double-stranded [32P]DNA prepared by nick translation from the cloned...

E S Tasheva; D J Roufa

1994-09-01T23:59:59.000Z

122

Redmond Red as a Redox Probe for the DNA-Mediated Detection of Abasic Sites  

Science Journals Connector (OSTI)

Redmond Red, a fluoropore containing a redox-active phenoxazine core, has been explored as a new electrochemical probe for the detection of abasic sites in double-stranded DNA. The electrochemical behavior of Redmond Red-modified DNA at gold surfaces ...

Marisa C. Buzzeo; Jacqueline K. Barton

2008-10-03T23:59:59.000Z

123

ATP Hydrolysis and DNA Binding by the Escherichia coli RecF Protein*  

E-Print Network [OSTI]

ATP Hydrolysis and DNA Binding by the Escherichia coli RecF Protein* (Received for publication ATP hydrolytic activity. ATP hydrolysis leads to RecF dissociation from double-stranded (ds to DNA. A mutant RecF protein that can bind but cannot hydrolyze ATP (RecF K36R) does not readily

Cox, Michael M.

124

Proteogenomic Analysis Reveals Unanticipated Adaptations of Colorectal Tumor Cells to Deficiencies in DNA Mismatch Repair  

Science Journals Connector (OSTI)

...related double-strand break repair protein NBN, which are constituents...decreased DNA synthesis and repair in MMR- cells (Fig. 4...application of our newly developed pipeline for the detection of variant...in DNA and RNA synthesis and repair. The driving proteins behind...

Patrick J. Halvey; Xiaojing Wang; Jing Wang; Ajaz A. Bhat; Punita Dhawan; Ming Li; Bing Zhang; Daniel C. Liebler; and Robbert J.C. Slebos

2014-01-01T23:59:59.000Z

125

Derivatized versions of ligase enzymes for constructing DNA sequences  

SciTech Connect (OSTI)

A method of making very long, double-stranded synthetic poly-nucleotides. A multiplicity of short oligonucleotides is provided. The short oligonucleotides are sequentially hybridized to each other. Enzymatic ligation of the oligonucleotides provides a contiguous piece of PCR-ready DNA of predetermined sequence.

Mariella, Jr., Raymond P. (Danville, CA); Christian, Allen T. (Tracy, CA); Tucker, James D. (Novi, MN); Dzenitis, John M. (Livermore, CA); Papavasiliou, Alexandros P. (Oakland, CA)

2006-08-15T23:59:59.000Z

126

Structure of the hexameric HerA ATPase reveals a mechanism of translocation-coupled DNA-end processing in archaea  

E-Print Network [OSTI]

. In the AMP-PNP bound state observed in the HerA structure, the second trans-acting arginine, R381, forms a salt-bridge with E356 of the Walker-B motif. This interaction is deemed necessary for both stable interface formation and water activation prior... annular components would form a coaxial structure, able to thread the DNA substrate through the HerA motor and towards the NurA nuclease. We surmise that DNA would enter the HerA ring via the positively-charged face formed by the helix-bundle domains...

Rzechorzek, Neil J.; Blackwood, John K.; Bray, Sian M.; Maman, Joseph D.; Pellegrini, Luca; Robinson, Nicholas P.

2014-01-01T23:59:59.000Z

127

Efficient and simpler method to construct normalized cDNA libraries with improved representations of full-length cDNAs  

DOE Patents [OSTI]

This invention provides a method to normalize a cDNA library comprising: (a) constructing a directionally cloned library containing cDNA inserts wherein the insert is capable of being amplified by polymerase chain reaction; (b) converting a double-stranded cDNA library into single-stranded DNA circles; (c) generating single-stranded nucleic acid molecules complementary to the single-stranded DNA circles converted in step (b) by polymerase chain reaction with appropriate primers; (d) hybridizing the single-stranded DNA circles converted in step (b) with the complementary single-stranded nucleic acid molecules generated in step (c) to produce partial duplexes to an appropriate Cot; and (e) separating the unhybridized single-stranded DNA circles from the hybridized DNA circles, thereby generating a normalized cDNA library. This invention also provides a method to normalize a cDNA library wherein the generating of single-stranded nucleic acid molecules complementary to the single-stranded DNA circles converted in step (b) is by excising cDNA inserts from the double-stranded cDNA library; purifying the cDNA inserts from cloning vectors; and digesting the cDNA inserts with an exonuclease. This invention further provides a method to construct a subtractive cDNA library following the steps described above. This invention further provides normalized and/or subtractive cDNA libraries generated by the above methods.

Soares, Marcelo Bento (New York, NY); Bonaldo, Maria de Fatima (New York, NY)

1998-01-01T23:59:59.000Z

128

1 Dda Helicase Tightly Couples Translocation on 2 Single-Stranded DNA to Unwinding of Duplex DNA  

E-Print Network [OSTI]

, Slot 516, Little Rock, AR 72205, USA 9 2 Department of Chemistry, Harding University, 915 East Market Avenue, Searcy, AR 72143, USA 10 3 Laboratorie de Physique Statistique, Ecole Normale Superieure, UPMC 25 Helicases utilize the energy of ATP hydrolysis to unwind double- 26 stranded DNA while

129

Neutrinoless Double Phys 135c Spring 2007  

E-Print Network [OSTI]

Neutrinoless Double Beta Decay Phys 135c Spring 2007 Michael Mendenhall #12;Theory Overview #12 beta decays #12;neutrinoless double beta decays n e- p beta decay e #12;neutrinoless double beta decays n e- p beta decay e n e- p n e- p double beta decay e e #12;neutrinoless double beta decays n e- p

Golwala, Sunil

130

DNA Nanotechnology- Architechtures Designed with DNA.  

E-Print Network [OSTI]

??As the genetic information storage vehicle, deoxyribonucleic acid (DNA) molecules are essential to all known living organisms and many viruses. It is amazing that such… (more)

Han, Dongran

2012-01-01T23:59:59.000Z

131

The Monoclinic Double Sulphates Containing Ammonium. Completion of the Double Sulphate Series  

Science Journals Connector (OSTI)

...Double Sulphates Containing Ammonium. Completion of the Double Sulphate Series A. E...properties physical properties refractive index sulfates GeoRef, Copyright 2007, American...double sulphates containing ammonium; completion of the double sulphate series Tutton...

1916-01-01T23:59:59.000Z

132

Monitoring a genetically engineered bacterium in a freshwater environment by rapid enzymatic amplification of a synthetic DNA “number-plate”  

Science Journals Connector (OSTI)

In order to set up a sensitive and reliable detection method to monitor environmentally released genetically engineered microorganisms (GEMs) a 72-bp, double-stranded DNA fragment has been built by annealing and ...

Augusto Amici; Marco Bazzicalupo; Enzo Gallori…

1991-11-01T23:59:59.000Z

133

Double acting bit holder  

DOE Patents [OSTI]

A double acting bit holder that permits bits held in it to be resharpened during cutting action to increase energy efficiency by reducing the amount of small chips produced. The holder consist of: a stationary base portion capable of being fixed to a cutter head of an excavation machine and having an integral extension therefrom with a bore hole therethrough to accommodate a pin shaft; a movable portion coextensive with the base having a pin shaft integrally extending therefrom that is insertable in the bore hole of the base member to permit the moveable portion to rotate about the axis of the pin shaft; a recess in the movable portion of the holder to accommodate a shank of a bit; and a biased spring disposed in adjoining openings in the base and moveable portions of the holder to permit the moveable portion to pivot around the pin shaft during cutting action of a bit fixed in a turret to allow front, mid and back positions of the bit during cutting to lessen creation of small chip amounts and resharpen the bit during excavation use.

Morrell, Roger J. (Blommington, MN); Larson, David A. (Minneapolis, MN); Ruzzi, Peter L. (Eagan, MN)

1994-01-01T23:59:59.000Z

134

Remarkable optics of short-pitch deformed helix ferroelectric liquid crystals: symmetries, exceptional points and polarization-resolved angular patterns  

E-Print Network [OSTI]

In order to explore electric-field-induced transformations of polarization singularities in the polarization-resolved angular (conoscopic) patterns emerging after deformed helix ferroelectric liquid crystal (DHFLC) cells with subwavelength helix pitch, we combine the transfer matrix formalism with the results for the effective dielectric tensor of biaxial FLCs evaluated using an improved technique of averaging over distorted helical structures. Within the framework of the transfer matrix method, we deduce a number of symmetry relations and show that the symmetry axis of L lines (curves of linear polarization) is directed along the major in-plane optical axis which rotates under the action of the electric field. When the angle between this axis and the polarization plane of incident linearly polarized light is above its critical value, the C points (points of circular polarization) appear in the form of symmetrically arranged chains of densely packed star-monstar pairs. We also emphasize the role of phase singularities of a different kind and discuss the enhanced electro-optic response of DHFLCs near the exceptional point where the condition of zero-field isotropy is fulfilled.

Alexei D. Kiselev; Vladimir G. Chigrinov

2014-07-14T23:59:59.000Z

135

A Twist-Bend Chiral Helix of 8nm Pitch in a Nematic Liquid Crystal of Achiral Molecular Dimers  

E-Print Network [OSTI]

Freeze Fracture Transmission Electron Microscopy (FFTEM) study of the nanoscale structure of the so-called "twist-bend" nematic (NX) phase of the cyanobiphenyl (CB) dimer molecule CB(CH2)7CB reveals a stripe texture of fluid layers periodically arrayed with a bulk spacing of d ~ 8.3 nm. Fluidity and a rigorously maintained spacing produce long-range-ordered fluid layered focal conic domains. Absence of a lamellar x-ray reflection at wavevector q ~ 2{\\pi}/8 nm-1 or its harmonics in synchrotron-based scattering experiments indicates that this periodic structure is achieved with no detectable associated modulation of the electron density, and thus has nematic molecular ordering. A search for periodic ordering with d ~ 8nm in CB(CH2)7CB using atomistic molecular dynamic computer simulation yielded equilibration of a conical twist-bend helixed nematic ground state, of the sort first proposed by Meyer, and envisioned in systems of bent molecules by Dozov and Memmer, We identify {\\theta} ~ 33 degree as the cone angle, and p ~ 8nm as the full pitch of the helix, the shortest ever found in a nematic fluid.

Dong Chen; Jan H. Porada; Justin B. Hooper; Arthur Klittnick; Yongqiang Shen; Eva Korblova; Dmitry Bedrov; David M. Walba; Matthew A. Glaser; Joseph E. Maclennan; Noel A. Clark

2013-06-24T23:59:59.000Z

136

APOBEC3G: a Double Agent in Defense Harold C. Smith  

E-Print Network [OSTI]

APOBEC3G: a Double Agent in Defense Harold C. Smith Department of Biochemistry and Biophysics to A3G having a requirement for a ssDNA Corresponding Author contacts: Harold C. Smith, Department and Dentistry, Rochester, NY 14642 USA, harold.smith@rochester.edu Voice: (585) 275-4267, FAX: (585) 275

Levin, Judith G.

137

Structure of the Full-Length Human RPA14/32 Complex Gives Insights Into the Mechanism of DNA Binding And Complex Formation  

SciTech Connect (OSTI)

Replication protein A (RPA) is the ubiquitous, eukaryotic single-stranded DNA (ssDNA) binding protein and is essential for DNA replication, recombination, and repair. Here, crystal structures of the soluble RPA heterodimer, composed of the RPA14 and RPA32 subunits, have been determined for the full-length protein in multiple crystal forms. In all crystals, the electron density for the N-terminal (residues 1--42) and C-terminal (residues 175--270) regions of RPA32 is weak and of poor quality indicating that these regions are disordered and/or assume multiple positions in the crystals. Hence, the RPA32 N terminus, that is hyperphosphorylated in a cell-cycle-dependent manner and in response to DNA damaging agents, appears to be inherently disordered in the unphosphorylated state. The C-terminal, winged helix-loop-helix, protein-protein interaction domain adopts several conformations perhaps to facilitate its interaction with various proteins. Although the ordered regions of RPA14/32 resemble the previously solved protease-resistant core crystal structure, the quaternary structures between the heterodimers are quite different. Thus, the four-helix bundle quaternary assembly noted in the original core structure is unlikely to be related to the quaternary structure of the intact heterotrimer. An organic ligand binding site between subunits RPA14 and RPA32 was identified to bind dioxane. Comparison of the ssDNA binding surfaces of RPA70 with RPA14/32 showed that the lower affinity of RPA14/32 can be attributed to a shallower binding crevice with reduced positive electrostatic charge.

Deng, X.; Habel, J.E.; Kabaleeswaran, V.; Snell, E.H.; Wold, M.S.; Borgstahl, G.E.O.

2009-06-03T23:59:59.000Z

138

Automating DNA processing  

E-Print Network [OSTI]

Center are exploring the potential of sequencing by hybridization (3). They are developing electronically addressable fixtures to contain immobilized synthetic DNA probes that can bind to target DNA samples. They call these microfabricated devices... Center are exploring the potential of sequencing by hybridization (3). They are developing electronically addressable fixtures to contain immobilized synthetic DNA probes that can bind to target DNA samples. They call these microfabricated devices...

Wienen, Michael Jan

2012-06-07T23:59:59.000Z

139

Covalently Linked DNA Nanotubes  

Science Journals Connector (OSTI)

SEM analyses of the nanotubes generated according to Scheme 2 further support the suggested folding of the 2D cross-linked DNA array into the nanotube structure. ... Here, we report a modular approach to DNA nanotube synthesis that provides access to geometrically well-defined triangular and square-shaped DNA nanotubes. ... and assembly of carbon nanotubes, and in nanotube-based DNA sensing and sepns. ...

Ofer I. Wilner; Anja Henning; Bella Shlyahovsky; Itamar Willner

2010-03-17T23:59:59.000Z

140

DNA nanotechnology: a future perspective  

Science Journals Connector (OSTI)

The rise of DNA-based nanobiotechnology has led to an increase in demand for synthetic DNA. DNA can be synthesized from nucleotides into ... technology has been the significant error rate of synthetic DNA sequenc...

Muniza Zahid; Byeonghoon Kim; Rafaqat Hussain; Rashid Amin…

2013-03-01T23:59:59.000Z

Note: This page contains sample records for the topic "dna double helix" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


141

Natural DNA sequencing by synthesis  

E-Print Network [OSTI]

labeling of a synthetic DNA template. By combining the bestpolyadenylation of a 100-base synthetic DNA template (nSBST)

Roller, Eric E.

2011-01-01T23:59:59.000Z

142

Neutrinoless Double Beta-Decay  

E-Print Network [OSTI]

The neutrinoless double $\\beta$-decay of nuclei is reviewed. We discuss neutrino mixing and 3x3 PMNS neutrino mixing matrix. Basic theory of neutrinoless double $\\beta$-decay is presented in some details. Results of different calculations of nuclear matrix element are discussed. Experimental situation is considered. The Appendix is dedicated to E. Majorana (brief biography and his paper in which the theory of Majorana particles is given)

S. M. Bilenky

2010-01-12T23:59:59.000Z

143

DNA Sequencing apparatus  

DOE Patents [OSTI]

An automated DNA sequencing apparatus having a reactor for providing at least two series of DNA products formed from a single primer and a DNA strand, each DNA product of a series differing in molecular weight and having a chain terminating agent at one end; separating means for separating the DNA products to form a series bands, the intensity of substantially all nearby bands in a different series being different, band reading means for determining the position an This invention was made with government support including a grant from the U.S. Public Health Service, contract number AI-06045. The U.S. government has certain rights in the invention.

Tabor, Stanley (Cambridge, MA); Richardson, Charles C. (Chestnut Hill, MA)

1992-01-01T23:59:59.000Z

144

Lattice ribbons: A model of double-stranded polymers  

Science Journals Connector (OSTI)

We introduce a discrete ribbon model for double-stranded polymers (such as duplex DNA) where the ribbon is constrained to lie on the simple cubic lattice Z3. The ribbon is made up of a sequence of plaquettes and can either be open or closed. We investigate the growth of the number of ribbons as a function of the number of plaquettes and use Monte Carlo methods to estimate the dimensions of the ribbon, the writhe of the backbone and, in the case of orientable closed ribbons, the linking number of the two boundary curves.

E.J. Janse van Rensburg; E. Orlandini; D.W Sumners; M.C. Tesi; S.G. Whittington

1994-12-01T23:59:59.000Z

145

Thermodynamic relations for DNA phase transitions  

E-Print Network [OSTI]

The force induced unzipping transition of a double stranded DNA is considered from a purely thermodynamic point of view. This analysis provides us with a set of relations that can be used to test microscopic theories and experiments. The thermodynamic approach is based on the hypothesis of impenetrability of the force in the zipped state. The melting and the unzipping transitions are considered in the same framework and compared with the existing statistical model results. The analysis is then extended to a possible continuous unzipping transition.

Poulomi Sadhukhan; Somendra M. Bhattacharjee

2014-01-21T23:59:59.000Z

146

MDC1 Cleavage by Caspase-3: A Novel Mechanism for Inactivating the DNA Damage Response during Apoptosis  

Science Journals Connector (OSTI)

...double-strand break repair and may be a novel therapeutic...commonly used or are in the pipeline, yet virtually all pancreatic...these lines, the DNA repair pathway is an attractive...timely drug development pipeline. Therefore, the model...components of the DNA repair complex (31, 33...

Stéphanie Solier and Yves Pommier

2011-02-01T23:59:59.000Z

147

IMPORTANCE OF NEUTRINOLESS DOUBLE BETA DECAY Abstract  

E-Print Network [OSTI]

number violation in nature and what is its magnitude. The neutrinoless double beta decay experiment can

U. Sarkar; Utpal Sarkar

148

Has globalization strengthened South Korea's national research system? National and international dynamics of the Triple Helix of scientific co-authorship relationships in South Korea  

Science Journals Connector (OSTI)

We trace the structural patterns of co-authorship between Korean researchers at three institutional types (university, government, and industry) and their international partners in terms of the mutual information generated in these relations. Data were ... Keywords: Co-authorship, Globalization, Innovation, International collaboration, National research system, R&D internationalization, South Korea, Triple Helix, University---industry---government relationship

Ki-Seok Kwon; Han Woo Park; Minho So; Loet Leydesdorff

2012-01-01T23:59:59.000Z

149

Polarization gratings in the short-pitch approximation: electro-optics of deformed helix ferroelectric liquid crystals  

E-Print Network [OSTI]

Electro-optical properties of deformed helix ferroelectric liquid crystal (DHFLC) cells are studied by using a general theoretical approach to polarization gratings in which the transmission and reflection matrices of diffraction orders are explicitly related to the evolution operator of equations for the Floquet harmonics. In the short-pitch approximation, a DHFLC cell is shown to be optically equivalent to a uniformly anisotropic biaxial layer where one of the optical axes is normal to the substrates. For in-plane anisotropy, orientation of the optical axes and birefringence are both determined by the voltage applied across the cell and represent the parameters that govern the transmittance of normally incident light passing through crossed polarizers. We calculate the transmittance as a function of the applied voltage and compare the computed curves with the experimental data. The theoretical and experimental results are found to be in good agreement.

Alexei D. Kiselev; Eugene P. Pozhidaev; Vladimir G. Chigrinov; Hoi-Sing Kwok

2010-11-19T23:59:59.000Z

150

Analysis of the interband optical transitions: Characterization of synthetic DNA band structure  

Science Journals Connector (OSTI)

We analyze the band structure and interband optical transitions in a dangling backbone ladder DNAmodel. Using this model semiconducting synthetic poly(G)-poly(C) DNA is studied by means of a tight-binding model traditionally used for transport studies. Numerical calculations for optical absorptionspectra are also presented. By studying the eigenstates’ symmetries in uniform and nonuniform DNA chains we conclude that in both cases the transitions are almost vertical in K space. The optical gap turns out larger than the electronic one and an indirect band gap electronic structure for this DNAmodel is revealed. The effects of the environment which are relevant for the wet form of DNA are taken into account by introducing disorder in the backbone levels. We demonstrate that they affect more the spectra in the case of parallel polarization of the incoming light (with respect to the molecule axis). In such a case the closure of the gap appears for a large enough disorder. We also consider the natural helix DNA conformation and find unusual selection rules for interband optical transitions. We propose that a comparison between the obtained spectra and the experiments can provide an insight into the electronic band structure of DNA.

Elena Dķaz

2008-01-01T23:59:59.000Z

151

Asymmetric DNA recognition by the OkrAI endonuclease, an isoschizomer of BamHI  

SciTech Connect (OSTI)

Restriction enzymes share little or no sequence homology with the exception of isoschizomers, or enzymes that recognize and cleave the same DNA sequence. We present here the structure of a BamHI isoschizomer, OkrAI, bound to the same DNA sequence (TATGGATCCATA) as that cocrystallized with BamHI. We show that OkrAI is a more minimal version of BamHI, lacking not only the N- and C-terminal helices but also an internal 310 helix and containing {beta}-strands that are shorter than those in BamHI. Despite these structural differences, OkrAI recognizes the DNA in a remarkably similar manner to BamHI, including asymmetric contacts via C-terminal 'arms' that appear to 'compete' for the minor groove. However, the arms are shorter than in BamHI. We observe similar DNA-binding affinities between OkrAI and BamHI but OkrAI has higher star activity (at 37 C) compared to BamHI. Together, the OkrAI and BamHI structures offer a rare opportunity to compare two restriction enzymes that work on exactly the same DNA substrate.

Vanamee, Éva Scheuring; Viadiu, Hector; Chan, Siu-Hong; Ummat, Ajay; Hartline, Adrian M.; Xu, Shuang-yong; Aggarwal, Aneel K. (Mount Sinai Hospital); (NEB)

2011-11-18T23:59:59.000Z

152

Meta-DNA: synthetic biology via DNA nanostructures and  

E-Print Network [OSTI]

Meta-DNA: synthetic biology via DNA nanostructures and hybridization reactions Harish Chandran1 strands and may be modified to allow for mutations. Keywords: DNA self-assembly; synthetic biology; DNA nanostructures 1. INTRODUCTION 1.1. Synthetic biology using DNA nanosystems A major goal of synthetic biology

Reif, John H.

153

Controlling DNA Methylation  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Controlling DNA Methylation Though life on earth is composed of a diverse range of organisms, some with many different types of tissues and cells, all these are encoded by a molecule we call DNA. The information required to build a protein is stored in DNA within the cells. Not all the message in the DNA is used in each cell and not all the message is used all the time. During cell differentiation, the cells become dedicated for their specific function which involves selectively activating some genes and repressing others. Gene regulation is an important event in the developmental biology and the biology of various diseases, but a more complex process. Controlling DNA Methylation Though life on earth is composed of a diverse range of organisms, some with many different types of tissues and cells, all these are encoded by a molecule we call DNA. The information required to build a protein is stored in DNA within the cells. Not all the message in the DNA is used in each cell and not all the message is used all the time. During cell differentiation, the cells become dedicated for their specific function which involves selectively activating some genes and repressing others. Gene regulation is an important event in the developmental biology and the biology of various diseases, but a more complex process. In the bacteria there are distinct enzymes while one is capable of cleaving DNA, the other protects DNA by modification. The complementary function provided by the set of enzymes offers a defense mechanism against the phage infection and DNA invasion. The incoming DNA is cleaved sequence specifically by the class of enzymes called restriction endonuclease (REase). The host DNA is protected by the sequence specific action of matching set of enzymes called the DNA methyltransferase (MTase). The control of the relative activities of the REase and MTase is critical because a reduced ratio of MTase/REase activity would lead to cell death via autorestriction. However too high a ratio would fail to provide protection against invading viral DNA. In addition a separate group of proteins capable of controlling R-M proteins have been identified in various restriction-modification (R-M) systems which are called C proteins (Roberts et al., 2003).

154

DNA's Role with Proteins  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

DNA's Role with Proteins DNA's Role with Proteins Name: Hans Location: N/A Country: N/A Date: N/A Question: Is it sure that the most important information of living cells is stored in the DNA? DNA seems to be nothing more than an inventory of useful proteins and a tool to create those proteins. Could it be that more important operational know how of how these proteins interact to build a living organism is actually located in the rest of the cell? So that the rest of the cell is the most important inheritance, whereas DNA merely takes care of the genetic variation? Replies: DNA is the entire library of protein information for an organism. All seven types of protein. It is true that in developmental stages of an organism that the presence and absences of certain proteins and other chemicals generated by proteins will influence what the DNA in a "particular" cell will express. Hence, you can start out with one cell and end up with a complex organism. You may have heard some of this information with the cloning activities that have been going on lately. All the inheritance comes from the DNA, but what parts of the DNA expression may be dictated by the cells special characteristics developed upon specializing. In that way the liver cells will only do "liver" things and the kidney cells will only do "kidney" things, BUT they use the same DNA information to operate, just a different portion of the same DNA that pertains to their particular "job". If you can convince a cell that it does not have a special job anymore, then you can develop the entire organism from this cell with the right signals; this is what cloning techniques have done!

155

Decimal System and Double Digits  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Decimal System and Double Digits Decimal System and Double Digits Name: Ken Status: other Grade: other Country: Canada Date: April 2011 Question: If the origin of the decimal system reflects counting on ten fingers and if zero came into use after the decimal system had been established why did we not create a single symbol for our tenth digit rather than use the double digit 10? If T were to represent the tenth number this would have created a counting system where the number series 1,2...9,T is followed by the same series having a 1 to the left then followed by the same series having a 2 to the left, etc. The T would be the last number in a series of ten single digits rather than be the first number in a series of double digits. The symbol zero would be used only between negative one and positive one because it represents the existence of nothing and, therefore, would have no other function.

156

The mechanism of human tyrosyl-DNA phosphodiesterase 1 in the cleavage of AP site and its synthetic analogs  

Science Journals Connector (OSTI)

Abstract The mechanism of hydrolysis of the apurinic/apyrimidinic (AP) site and its synthetic analogs by using tyrosyl-DNA phosphodiesterase 1 (Tdp1) was analyzed. Tdp1 catalyzes the cleavage of AP site and the synthetic analog of the AP site, 3-hydroxy-2(hydroxymethyl)-tetrahydrofuran (THF), in DNA by hydrolysis of the phosphodiester bond between the substituent and 5? adjacent phosphate. The product of Tdp1 cleavage in the case of the AP site is unstable and is hydrolyzed with the formation of 3?- and 5?-margin phosphates. The following repair demands the ordered action of polynucleotide kinase phosphorylase, with XRCC1, DNA polymerase ?, and DNA ligase. In the case of THF, Tdp1 generates break with the 5?-THF and the 3?-phosphate termini. Tdp1 is also able to effectively cleave non-nucleotide insertions in DNA, decanediol and diethyleneglycol moieties by the same mechanism as in the case of THF cleavage. The efficiency of Tdp1 catalyzed hydrolysis of AP-site analog correlates with the DNA helix distortion induced by the substituent. The following repair of 5?-THF and other AP-site analogs can be processed by the long-patch base excision repair pathway.

Natalia A. Lebedeva; Nadejda I. Rechkunova; Alexander A. Ishchenko; Murat Saparbaev; Olga I. Lavrik

2013-01-01T23:59:59.000Z

157

DNA | Open Energy Information  

Open Energy Info (EERE)

DNA DNA Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home NEPA Casual Use Determination of NEPA Adequacy Categorical Exclusion Environmental Assessment Environmental Impact Statements Print PDF NEPA-Related Analysis: Determination of NEPA Adequacy (DNA) General Document Collections (28) Documents Regulatory Roadmap NEPA-Related Analysis: Determination of NEPA Adequacy and Land Use Plan Conformance (DNA) placeholder. This query has been included to allow you to use the black arrows in the table header cells to sort the table data. Document # Serial Number Applicant Lead Agency District Office Field Office Development Phase(s) Techniques DOI-BLM-NM-L000-2012-0020-DNA Lightning Dock Geothermal Inc BLM BLM Las Cruces District Office BLM Geothermal/Exploration

158

NEUTRINOLESS DOUBLE BETA DECAY AND ITS “INVERSE”  

E-Print Network [OSTI]

subsequently published arguments, non-observation of neutrinoless double beta decay has, to date, no bearing on

Clemens A. Heusch; Peter Minkowski

1996-01-01T23:59:59.000Z

159

Neutrinoless Double Beta Decay and CP Violation  

E-Print Network [OSTI]

neutrinoless double beta decay in the case of two neutrino generations (or when the third generation leptons do

Patrick J. O’donnell; Utpal Sarkar

1993-01-01T23:59:59.000Z

160

An autoinhibitory helix in the C-terminal region of phospholipase C-[beta] mediates G[alpaha subscript q] activation  

SciTech Connect (OSTI)

The enzyme phospholipase C-{beta} (PLC{beta}) is a crucial regulator of intracellular calcium levels whose activity is controlled by heptahelical receptors that couple to members of the G{sub q} family of heterotrimeric G proteins. We have determined atomic structures of two invertebrate homologs of PLC{beta} (PLC21) from cephalopod retina and identified a helix from the C-terminal regulatory region that interacts with a conserved surface of the catalytic core of the enzyme. Mutations designed to disrupt the analogous interaction in human PLC{beta}3 considerably increase basal activity and diminish stimulation by G{alpha}{sub q}. G{alpha}{sub q} binding requires displacement of the autoinhibitory helix from the catalytic core, thus providing an allosteric mechanism for activation of PLC{beta}.

Lyon, Angeline M.; Tesmer, Valerie M.; Dhamsania, Vishan D.; Thal, David M.; Gutierrez, Joanne; Chowdhury, Shoaib; Suddala, Krishna C.; Northup, John K.; Tesmer, John J.G. (Michigan); (NIH)

2012-03-16T23:59:59.000Z

Note: This page contains sample records for the topic "dna double helix" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


161

Characterization of a parallel-stranded DNA hairpin  

SciTech Connect (OSTI)

Recently, the authors have shown that synthetic DNA containing homooligomeric A-T base pairs can form a parallel-stranded intramolecular hairpin structure. In the present study, they have employed NMR and optical spectroscopy to investigate the structure of the parallel-stranded (PS) DNA hairpin 3{prime}-d(T){sub 8}C{sub 4}(A){sub 8}-3{prime} and the related antiparallel (APS) hair 5{prime}-d(T){sub 8}C{sub 4}(A){sub 8}-3{prime}. The parallel orientation of the strands in the PS oligonucleotide is achieved by introducing a 5{prime}-5{prime} phosphodiester linkage in the hairpin loop. Ultraviolet spectroscopic and fluorescence data of drug binding are consistent with the formation of PS and APS structures, respectively, in these two hairpins. Vacuum circular dichroism measurements in combination with theoretical CD calculations indicate that the PS structure forms a right-handed helix. {sup 31}P NMR measurements indicate that the conformation of the phosphodiester backbone of the PS structure is not drastically different from that of the APS control. The presence of slowly exchanging imino protons at 14 ppm and the observation of nuclear Overhauser enhancement between imino protons and the AH-2 protons demonstrate that similar base pairing and base stacking between T and A residues occur in both hairpins. On the basis of NOESY measurements, they find that the orientation of the bases is in the anti region and that the sugar puckering is in the 2{prime}-endo range. The results indicate a B-like conformation for each of the strands in the stem part of the PS hairpin and reverse Watson-Crick base pairing between the T and A residues. These data are consistent with a previously calculated structure for parallel-stranded DNA.

Germann, M.W.; Vogel, H.J.; Pon, R.T.; van de Sande, J.H. (Univ. of Calgary, Alberta (Canada))

1989-07-25T23:59:59.000Z

162

Supramolecular DNA nanotechnology : discrete nanoparticle organization, three-dimensional DNA construction, and molecule templated DNA assembly.  

E-Print Network [OSTI]

??The field of structural DNA nanotechnology utilizes DNA's powerful base-pairing molecular recognition criteria to help solve real challenges facing researchers in material science and nanotechnology,… (more)

Aldaye, Faisal A., 1979-

2008-01-01T23:59:59.000Z

163

Doubling of atmospheric methane supported  

SciTech Connect (OSTI)

Atmospheric methane over the past 27,000 years was measured by analyzing air trapped in glacial ice in Greenland and Antarctica. Atmospheric concentrations were stable over that period until about 200 years b.p. In the last 200 years they have more than doubled. This change in concentration is correlated with the increase in human population; the implications for climate modification are discussed. 1 figure, 3 references.

Kerr, R.A.

1984-11-23T23:59:59.000Z

164

Photophysical Characterization of a FRET System Using Tailor-Made DNA Oligonucleotide Sequences  

Science Journals Connector (OSTI)

We have carried out a detailed photophysical study of the FRET D/A pair consisting of a carbostyril donor and a Ru(II)bathophenanthroline complex acceptor in double-stranded synthetic DNA. ... Feasibility studies for the FRET system were first carried out in peptides (30, 31) and later also in synthetic DNA (32, 33). ... The unique combination of chemically robust dye-labeled building blocks and remarkable spectroscopic properties of the FRET system prompted us to perform a detailed study concerning synthetic DNA, in which the distance between donor and acceptor was systematically varied by the number of separating base pairs. ...

Roman Flehr; Andrea Kienzler; Willi Bannwarth; Michael U. Kumke

2010-11-29T23:59:59.000Z

165

Autonomous Programmable Biomolecular Devices Using Self-Assembled DNA Nanostructures  

E-Print Network [OSTI]

-Assembled DNA Nanostructures: Ā· use synthetic DNA to self-assemble into DNA nanostructure devices. Goals

Reif, John H.

166

Divalent Ion and Thermally Induced DNA Conformational Polymorphism on Single-walled  

E-Print Network [OSTI]

coverage on nanotubes dispersed in aqueous solution decreases, the emission energy decreases (due Nanotubes Hong Jin1 , Esther S. Jeng1 , Daniel A. Heller2 , Prakrit V. Jena3 , Robert Kirmse4 , Jƶrg and double stranded DNA can wrap and colloidally stabilize single walled carbon nanotubes in solution

Langowski, Jƶrg

167

1 15/03/2006 Complexity of excited state dynamics in DNA  

E-Print Network [OSTI]

of model DNA helices using femtosecond transient absorption spectroscopy1 and by observing been assigned to excimer emission9, 10 . We observe that, in contrast to the transient absorption of these single and double strands at identical wavelengths. Neither in fluorescence nor in transient absorption

Boyer, Edmond

168

Chronic hepatitis in chimpanzee carriers of hepatitis B virus: morphologic, immunologic, and viral DNA studies  

Science Journals Connector (OSTI)

...core antigen (anti-HBc), and Dane particles in the serum. Therefore, it appeared...Acad. Sc. USA 77 (1980) 6149 _ 4 ' God. wl., CA _> ~ ~ ~~~~ - -4o...represents fully double-stranded Dane particle DNA. Characterization of the 4100-base-pair...

D Shouval; P R Chakraborty; N Ruiz-Opazo; S Baum; I Spigland; E Muchmore; M A Gerber; S N Thung; H Popper; D A Shafritz

1980-01-01T23:59:59.000Z

169

Investigations of ?-helix??-sheet transition pathways in a miniprotein using the finite-temperature string method  

SciTech Connect (OSTI)

A parallel implementation of the finite-temperature string method is described, which takes into account the invariance of coordinates with respect to rigid-body motions. The method is applied to the complex ?-helix??-sheet transition in a ?-hairpin miniprotein in implicit solvent, which exhibits much of the complexity of conformational changes in proteins. Two transition paths are considered, one derived from a linear interpolant between the endpoint structures and the other derived from a targeted dynamics simulation. Two methods for computing the conformational free energy (FE) along the string are compared, a restrained method, and a tessellation method introduced by E. Vanden-Eijnden and M. Venturoli [J. Chem. Phys. 130, 194103 (2009)]. It is found that obtaining meaningful free energy profiles using the present atom-based coordinates requires restricting sampling to a vicinity of the converged path, where the hyperplanar approximation to the isocommittor surface is sufficiently accurate. This sampling restriction can be easily achieved using restraints or constraints. The endpoint FE differences computed from the FE profiles are validated by comparison with previous calculations using a path-independent confinement method. The FE profiles are decomposed into the enthalpic and entropic contributions, and it is shown that the entropy difference contribution can be as large as 10 kcal/mol for intermediate regions along the path, compared to 15–20 kcal/mol for the enthalpy contribution. This result demonstrates that enthalpic barriers for transitions are offset by entropic contributions arising from the existence of different paths across a barrier. The possibility of using systematically coarse-grained representations of amino acids, in the spirit of multiple interaction site residue models, is proposed as a means to avoid ad hoc sampling restrictions to narrow transition tubes.

Ovchinnikov, Victor, E-mail: ovchinnv@georgetown.edu [Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138 (United States)] [Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138 (United States); Karplus, Martin, E-mail: marci@tammy.harvard.edu [Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138 (United States) [Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138 (United States); Laboratoire de Chimie Biophysique, ISIS, Université de Strasbourg, 67000 Strasbourg (France)

2014-05-07T23:59:59.000Z

170

Efficient replication of a paramyxovirus independent of full zippering of the fusion protein six-helix bundle domain  

Science Journals Connector (OSTI)

...syncytial virus (BRSV) from cDNA: BRSV NS2 is not essential for virus replication...different viral families Protein Data Bank ID code Virus Viral family Residues in HR1...Paramyxoviridae 52 26 2 39 Protein Data Bank ID codes for the individual fusion cores are specified...

Melinda A. Brindley; Philippe Plattet; Richard Karl Plemper

2014-01-01T23:59:59.000Z

171

Reversed Doppler effect in double negative metamaterials  

Science Journals Connector (OSTI)

Doppler shifts in double negative metamaterials have never been observed. This Rapid Communication presents experimental results on Doppler effect in a double negative acoustic metamaterial. We observed that frequency was downshifted when the source was approaching and upshifted when receding. Notably, while in ordinary media wavelengths corresponding to downshifted frequencies are longer, we demonstrate that in double negative metamaterials wavelengths increase as the frequencies increase. Consequently even though the frequencies were downshifted in front of the moving source, the wavelengths became shorter.

Sam Hyeon Lee; Choon Mahn Park; Yong Mun Seo; Chul Koo Kim

2010-06-14T23:59:59.000Z

172

Double perovskite catalysts for oxidative coupling  

DOE Patents [OSTI]

Alkali metal doped double perovskites containing manganese and at least one of cobalt, iron and nickel are useful in the oxidative coupling of alkane to higher hydrocarbons.

Campbell, K.D.

1991-01-01T23:59:59.000Z

173

Review of double beta decay experiments  

E-Print Network [OSTI]

The brief review of current experiments on search and studying of double beta decay processes is done. Best present limits on $\\langle m_{\

A. S. Barabash

2014-03-12T23:59:59.000Z

174

Review of double beta decay experiments  

E-Print Network [OSTI]

The brief review of current experiments on search and studying of double beta decay processes is done. Best present limits on $\\langle m_{\

Barabash, A S

2014-01-01T23:59:59.000Z

175

Comparing Double String Theory Actions  

E-Print Network [OSTI]

Aimed to a deeper comprehension of a manifestly T-dual invariant formulation of string theory, in this paper a detailed comparison between the non-covariant action proposed by Tseytlin and the covariant one proposed by Hull is done. These are obtained by making both the string coordinates and their duals explicitly appear, on the same foot, in the world-sheet action, so "doubling" the string coordinates along the compact dimensions. After a discussion of the nature of the constraints in both the models and the relative quantization, it results that the string coordinates and their duals behave like "non-commuting" phase space type coordinates but their expressions in terms of Fourier modes generate the oscillator algebra of the standard bosonic string formulation. A proof of the equivalence of the two formulations is given. Furthermore, open-string solutions are also discussed.

Luca De Angelis; S. J. Gabriele Gionti; Raffaele Marotta; Franco Pezzella

2013-12-27T23:59:59.000Z

176

Review Paper. Ancient DNA  

Science Journals Connector (OSTI)

...poorly char- acterized (Gilbert et al. 2003a,b...research has been done in microbiology departments where the...unlikely to preserve DNA (Gilbert et al. 2005a). This...Smith, B. D., Gilbert, M. T. P., Cooper...the tenacity of life. Microbiology 140, 25132529. Kim...

2005-01-01T23:59:59.000Z

177

Recognition of DNA by Synthetic Antibodies  

Science Journals Connector (OSTI)

Recognition of DNA by Synthetic Antibodies ... The recombinant anti-ssDNA Fab, DNA-1, and 16 heavy chain complementarity determining region 3 (HCDR3) mutant variants were selected for thermodynamic characterization of ssDNA binding. ...

Shana M. Barbas; Peter Ghazal; Carlos F. Barbas III; Dennis R. Burton

1994-03-01T23:59:59.000Z

178

Nuclear DNA Amounts in Angiosperms  

Science Journals Connector (OSTI)

27 May 1976 research-article Nuclear DNA Amounts in Angiosperms M. D. Bennett...number of angiosperm species for which nuclear DNA amount estimates have been made has...is overdue. This paper lists absolute nuclear DNA amounts for 753 angiosperm species...

1976-01-01T23:59:59.000Z

179

DNA Structural Nanotechnology Duke University  

E-Print Network [OSTI]

DNA Structural Nanotechnology John Reif Duke University Graduate Students: Harish Chandran&Caltech Tube Lattices #12;Ned Seeman New York University, USA Ned Seeman: Father of DNA Nanotechnology His Initial Ideas & Motivation for DNA Nanotechnology #12;Cube Chen & Seeman, Nature350:631 (1991) Truncated

Reif, John H.

180

Heuristic for Maximizing DNA Reuse in Synthetic DNA Library Assembly  

Science Journals Connector (OSTI)

Heuristic for Maximizing DNA Reuse in Synthetic DNA Library Assembly ... In concert with entirely de novo synthesis, a swathe of alternative DNA assembly methods are being introduced(4-6) that concatenate parts from hundreds of base pairs in length, through gene fusions, to synthetic biological devices built from catalogued parts, up to megabase fragments and entire chromosomes. ... De novo synthesis relies on synthetic oligos that are inherently error prone, and therefore, reusing existing error-free DNA in constructing new DNA provides an inherent advantage. ...

Jonathan Blakes; Ofir Raz; Uriel Feige; Jaume Bacardit; Pawe? Widera; Tuval Ben-Yehezkel; Ehud Shapiro; Natalio Krasnogor

2014-02-20T23:59:59.000Z

Note: This page contains sample records for the topic "dna double helix" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


181

Double superexchange in quantum dot mesomaterials  

E-Print Network [OSTI]

as the critical obstacles to dramatic enhancements in the energy conversion efficiency of photovoltaic cells based.7Ā­10 A low binding energy allows excitons to thermally dissociate and charge carriers hop from dot exhibits a double superexchange. In addition to enhancing carrier hopping rates, double superexchange plays

Wu, Zhigang

182

Double beta decay: experiments and theory review  

E-Print Network [OSTI]

Neutrinoless double beta decay is one of the most powerful tools to set the neutrino mass absolute scale and establish whether the neutrino is a Majorana particle. After a summary of the neutrinoless double beta decay phenomenology, the present status of the experimental search for this rare decay is reported and the prospects for next generation experiments are reviewed.

A. Nucciotti

2007-07-15T23:59:59.000Z

183

The effect of magnesium ions on dielectric relaxation in semidilute DNA aqueous solutions  

E-Print Network [OSTI]

The effect of magnesium ion Mg2+ on the dielectric relaxation of semidilute DNA aqueous solutions has been studied by means of dielectric spectroscopy. Two dielectric relaxations in the 100 Hz - 100 MHz frequency range, originating in the motion of DNA counterions, were probed as a function of DNA and Mg2+ ion concentration in added MgCl2 salt. The high-frequency mode in the MHz range, stemming from the structural organization of the DNA network, reveals de Gennes-Pfeuty-Dobrynin correlation length as the pertinent fundamental length scale for sufficiently low concentration of added salt. No relaxation fingerprint of DNA denaturation bubbles, leading to exposed hydrophobic core scaling, was detected at low DNA concentrations, thus indicating an increased stability of the double-stranded conformation as compared to the case of DNA solutions with univalent counterions. The presence of Mg2+ does not change qualitatively the low frequency mode in the kHz range correlated with single DNA conformational properties....

Grgi?in, Danijel; Ivek, Tomislav; Tomi?, Silvia; Podgornik, Rudi

2013-01-01T23:59:59.000Z

184

Fleet DNA (Presentation)  

SciTech Connect (OSTI)

The Fleet DNA project objectives include capturing and quantifying drive cycle and technology variation for the multitude of medium- and heavy-duty vocations; providing a common data storage warehouse for medium- and heavy-duty vehicle fleet data across DOE activities and laboratories; and integrating existing DOE tools, models, and analyses to provide data-driven decision making capabilities. Fleet DNA advantages include: for Government - providing in-use data for standard drive cycle development, R&D, tech targets, and rule making; for OEMs - real-world usage datasets provide concrete examples of customer use profiles; for fleets - vocational datasets help illustrate how to maximize return on technology investments; for Funding Agencies - ways are revealed to optimize the impact of financial incentive offers; and for researchers -a data source is provided for modeling and simulation.

Walkokwicz, K.; Duran, A.

2014-06-01T23:59:59.000Z

185

What can we learn from neutrinoless double beta decay experiments?  

E-Print Network [OSTI]

312 P. Vogel, “Limits From Neutrinoless Double-Beta Decay (Assumptions No detected neutrinoless double ?-decay lightestscale (1 ± 0.05 eV), No neutrinoless double ?-decay lightest

Bahcall, John N.

2009-01-01T23:59:59.000Z

186

A Search for Neutrinoless Double Beta Decay of Te-130  

E-Print Network [OSTI]

Bolometric experiments for neutrinoless double beta 3.2.1A Search for Neutrinoless Double Beta Decay of Te by AdamSpring 2010 A Search for Neutrinoless Double Beta Decay of

Bryant, Adam Douglas

2010-01-01T23:59:59.000Z

187

Neutrinoless Double Beta Decay in Light of SNO Salt Data  

E-Print Network [OSTI]

14] P. Vogel, “Limits From Neutrinoless Double-Beta Decay (Higgs, can also cause neutrinoless double-beta decay (seeLBNL-53996 Neutrinoless Double Beta Decay in Light of SNO

Murayama, Hitoshi

2009-01-01T23:59:59.000Z

188

Chiral Mesophases of DNA  

E-Print Network [OSTI]

In the hexagonal columnar phase of chiral polymers a bias towards cholesteric twist competes with braiding along an average direction. When the chirality is strong, topological defects proliferate, leading to either a tilt grain boundary phase or a new ``moire state'' with twisted bond order. This moire phase can melt leading to a new phase: the chiral hexatic. I will discuss some recent experimental results from the NIH on DNA liquid crystals in the context of these theories.

Randall D. Kamien

1998-12-08T23:59:59.000Z

189

Radiation and viral DNA  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Radiation and viral DNA Radiation and viral DNA Name: Loretta L Lamb Age: N/A Location: N/A Country: N/A Date: N/A Question: Can viral DNA be changed through exposure to radiation? If so, what type of radiation will do this? Can these irradiated viruses cause changes in the genome of any human cells they may infect? Can these (or any) viruses actually cause cancer, or do they merely act as triggering devices for cancer? Replies: In theory, any nucleic acid (viral or otherwise) can be changed by exposure to many kinds of radiation. Depending on the type of virus, these may then change the human cells that they infect. Although there are many different things that are being implicated in causing cancers, it looks like a fairly common model involves the sequential "knockout" of several human genes. Viruses may be one cause of such gene changes, radiation and other environmental causes may also contribute. Some of these changes may be inherited through families, so it becomes more likely that the environmental factors may happen to "hit" the right places in cells to cause cancers in these families. If you ask something more specific, perhaps I can focus my response a bit more

190

Structural design of double hull tankers  

SciTech Connect (OSTI)

After a brief introduction of structural design procedure for double hull tankers, their design features are firstly highlighted comparing with conventional single hull tankers. Next discussed are recent topics of double hull VLCC (Very Large Crude oil Carrier) including higher tensile steel and strut arrangement in transverse ring frames. Finally ultimate longitudinal strength of hull girder is analyzed through a simplified method under both sagging and hogging conditions. Making use of this result, some considerations are made on structural reliability of double hull VLCC.

Niho, O.; Yanagibayashi, S.; Akashi, T. [Mitsui Engineering and Shipbuilding Co., Ltd., Tokyo (Japan)

1994-12-31T23:59:59.000Z

191

Smectic Order in Double-Twist Cylinders  

E-Print Network [OSTI]

I propose a double-twist texture with local smectic order, which may have been seen in recent experiments. As in the Renn-Lubensky TGB phase, the smectic order is broken only through a lattice of screw dislocations. A melted lattice of screw dislocations can produce a double-twist texture as can an unmelted lattice. In the latter case I show that geometry only allows for certain angles between smectic regions. I discuss the possibility of connecting these double-twist tubes together to form a smectic blue phase.

Randall D. Kamien

1996-11-04T23:59:59.000Z

192

Low Dose Radiation Induced DNA Damage Signaling and Repair Responses in  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Induced DNA Damage Signaling and Repair Responses in Induced DNA Damage Signaling and Repair Responses in Human 3-Dimensional Skin Model System Yanrong Su, Jarah Meador and Adayabalam S. Balajee Center for Radiological Research, College of Physicians and Surgeons, Columbia University, 630 West, 168th Street, New York, NY 10032. Exposure to ionizing radiation (IR) inflicts a wide variety of lesions in the genomic DNA. Among them, DNA double strand break (DSB) is considered to be the critical lesion for most of the deleterious radiation effects including carcinogenesis. Much of our knowledge on induction and repair kinetics of DSB has come from studies in two dimensional cell culture systems. However, the damage signaling and repair responses to DSB in tissue microenvironment are largely unknown. Knowledge of tissue responses to

193

Photo double ionization of ethylene and acetylene near threshold  

E-Print Network [OSTI]

Photo double ionization of ethylene and acetylene nearcomplete measurements of the photo double ionization ofkinetic energy of the photo electrons and the nuclear

Gaire, B.

2014-01-01T23:59:59.000Z

194

National CHP Roadmap: Doubling Combined Heat and Power Capacity...  

Broader source: Energy.gov (indexed) [DOE]

National CHP Roadmap: Doubling Combined Heat and Power Capacity in the United States by 2010, March 2001 National CHP Roadmap: Doubling Combined Heat and Power Capacity in the...

195

Photo double ionization of ethylene and acetylene near threshold  

E-Print Network [OSTI]

double ionization of ethylene and acetylene near thresholdphoto double ionization of ethylene (dou- ble CC bond) andsimilar yields of metastable ethylene dications produced by

Gaire, B.

2014-01-01T23:59:59.000Z

196

Molecular Dynamics Study of the Electrical Double Layer at Silver...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Electrical Double Layer at Silver Chloride Electrolyte Interfaces. Molecular Dynamics Study of the Electrical Double Layer at Silver Chloride Electrolyte Interfaces. Abstract:...

197

Search for: "neutrinoless double beta decay" | DOE PAGES  

Office of Scientific and Technical Information (OSTI)

neutrinoless double beta decay" Find + Advanced Search Advanced Search All Fields: "neutrinoless double beta decay" Title: Full Text: Bibliographic Data: Creator Author: Name...

198

Unscheduled DNA synthesis and mitochondrial DNA synthetic rate following injury of the facial nerve  

Science Journals Connector (OSTI)

Unscheduled DNA synthesis (UDS) of nuclear DNA and mitochondrial (mt) DNA synthetic rates were determined autoradiographically in different cell ... nerve transection. In addition to an increased synthetic rate ...

H. Korr; V. Philippi; C. Helg; J. Schiefer; M. B. Graeber…

1997-11-01T23:59:59.000Z

199

Double-Disk Dark Matter  

Science Journals Connector (OSTI)

Abstract Based on observational tests of large scale structure and constraints on halo structure, dark matter is generally taken to be cold and essentially collisionless. On the other hand, given the large number of particles and forces in the visible world, a more complex dark sector could be a reasonable or even likely possibility. This hypothesis leads to testable consequences, perhaps portending the discovery of a rich hidden world neighboring our own. We consider a scenario that readily satisfies current bounds that we call Partially Interacting Dark Matter (PIDM). This scenario contains self-interacting dark matter, but it is not the dominant component. Even if PIDM contains only a fraction of the net dark matter density, comparable to the baryonic fraction, the subdominant component’s interactions can lead to interesting and potentially observable consequences. Our primary focus will be the special case of Double-Disk Dark Matter (DDDM), in which self-interactions allow the dark matter to lose enough energy to lead to dynamics similar to those in the baryonic sector. We explore a simple model in which DDDM can cool efficiently and form a disk within galaxies, and we evaluate some of the possible observational signatures. The most prominent signal of such a scenario could be an enhanced indirect detection signature with a distinctive spatial distribution. Even though subdominant, the enhanced density at the center of the galaxy and possibly throughout the plane of the galaxy (depending on precise alignment) can lead to large boost factors, and could even explain a signature as large as the 130 GeV Fermi line. Such scenarios also predict additional dark radiation degrees of freedom that could soon be detectable and would influence the interpretation of future data, such as that from Planck and from the Gaia satellite. We consider this to be the first step toward exploring a rich array of new possibilities for dark matter dynamics.

JiJi Fan; Andrey Katz; Lisa Randall; Matthew Reece

2013-01-01T23:59:59.000Z

200

DUPLEX: A molecular mechanics program in torsion angle space for computing structures of DNA and RNA  

SciTech Connect (OSTI)

DUPLEX produces energy minimized structures of DNA and RNA of any base sequence for single and double strands. The smallest subunits are deoxydinucleoside monophosphates, and up to 12 residues, single or double stranded can be treated. In addition, it can incorporate NMR derived interproton distances an constraints in the minimizations. Both upper and lower bounds for these distances can be specified. The program has been designed to run on a UNICOS Cray supercomputer, but should run, albeit slowly, on a laboratory computer such as a VAX or a workstation.

Hingerty, B.E.

1992-07-01T23:59:59.000Z

Note: This page contains sample records for the topic "dna double helix" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


201

C-terminal region of DNA ligase IV drives XRCC4/DNA ligase IV complex to chromatin  

SciTech Connect (OSTI)

Highlights: •Chromatin binding of XRCC4 is dependent on the presence of DNA ligase IV. •C-terminal region of DNA ligase IV alone can recruit itself and XRCC4 to chromatin. •Two BRCT domains of DNA ligase IV are essential for the chromatin binding of XRCC4. -- Abstract: DNA ligase IV (LIG4) and XRCC4 form a complex to ligate two DNA ends at the final step of DNA double-strand break (DSB) repair through non-homologous end-joining (NHEJ). It is not fully understood how these proteins are recruited to DSBs. We recently demonstrated radiation-induced chromatin binding of XRCC4 by biochemical fractionation using detergent Nonidet P-40. In the present study, we examined the role of LIG4 in the recruitment of XRCC4/LIG4 complex to chromatin. The chromatin binding of XRCC4 was dependent on the presence of LIG4. The mutations in two BRCT domains (W725R and W893R, respectively) of LIG4 reduced the chromatin binding of LIG4 and XRCC4. The C-terminal fragment of LIG4 (LIG4-CT) without N-terminal catalytic domains could bind to chromatin with XRCC4. LIG4-CT with W725R or W893R mutation could bind to chromatin but could not support the chromatin binding of XRCC4. The ability of C-terminal region of LIG4 to interact with chromatin might provide us with an insight into the mechanisms of DSB repair through NHEJ.

Liu, Sicheng; Liu, Xunyue; Kamdar, Radhika Pankaj; Wanotayan, Rujira; Sharma, Mukesh Kumar [Research Laboratory for Nuclear Reactors and Department of Nuclear Engineering, Graduate School of Science and Engineering, Tokyo Institute of Technology, Tokyo 152-8550 (Japan)] [Research Laboratory for Nuclear Reactors and Department of Nuclear Engineering, Graduate School of Science and Engineering, Tokyo Institute of Technology, Tokyo 152-8550 (Japan); Adachi, Noritaka [Graduate School of Nanobioscience, Yokohama City University, Yokohama 236-0027 (Japan)] [Graduate School of Nanobioscience, Yokohama City University, Yokohama 236-0027 (Japan); Matsumoto, Yoshihisa, E-mail: yoshim@nr.titech.ac.jp [Research Laboratory for Nuclear Reactors and Department of Nuclear Engineering, Graduate School of Science and Engineering, Tokyo Institute of Technology, Tokyo 152-8550 (Japan)] [Research Laboratory for Nuclear Reactors and Department of Nuclear Engineering, Graduate School of Science and Engineering, Tokyo Institute of Technology, Tokyo 152-8550 (Japan)

2013-09-20T23:59:59.000Z

202

Double Beta Decay Constraint on Composite Neutrinos  

Science Journals Connector (OSTI)

......Constraint on Composite Neutrinos Eiichi Takasugi Department of Physics, Osaka University, Toyonaka 560 Neutrinoless double beta decay (betabeta)0v occurs through the magnetic coupling of dimension five operator whose coupling constant is......

Eiichi Takasugi

1997-10-01T23:59:59.000Z

203

Phenomenology of single and double diffraction dissociation  

E-Print Network [OSTI]

Predictions of the gap-probability renormalization model for single and double diffraction dissociation cross sections in proton-proton collisions at the LHC are presented and compared with recent CMS measurements.

Konstantin Goulianos

2013-10-21T23:59:59.000Z

204

Phenomenology of neutrinoless double beta decay  

E-Print Network [OSTI]

This paper reviews the current status and future outlook of neutrinoless double beta decay searches, which try to provide an answer to the fundamental question of whether neutrinos are Dirac or Majorana particles.

Gómez-Cadenas, J J

2015-01-01T23:59:59.000Z

205

Phenomenology of neutrinoless double beta decay  

E-Print Network [OSTI]

This paper reviews the current status and future outlook of neutrinoless double beta decay searches, which try to provide an answer to the fundamental question of whether neutrinos are Dirac or Majorana particles.

J. J. Gómez-Cadenas; Justo Martķn-Albo

2015-02-02T23:59:59.000Z

206

Double Pomeron Physics at the LHC  

E-Print Network [OSTI]

I discuss central exclusive production, otherwise known as Double Pomeron Exchange, DPE, from the ISR through the Tevatron to the LHC. There I emphasize the interest of exclusive Higgs and WW/ZZ production.

Michael G. Albrow

2005-07-25T23:59:59.000Z

207

Neutrinoless double beta decay with scalar bilinears  

E-Print Network [OSTI]

One possible probe to physics beyond the standard model is to look for scalar bilinears, which couple to two fermions of the standard model. We point out that the scalar bilinears allow new diagrams contributing to the neutrinoless double beta decay. The upper bound on the neutrinoless double beta decay lifetime would then give new constraints on the ratio of the masses of these scalars to their couplings to the fermions.

H. V. Klapdor-Kleingrothaus; U. Sarkar

2002-11-18T23:59:59.000Z

208

Wavelength-doubling optical parametric oscillator  

DOE Patents [OSTI]

A wavelength-doubling optical parametric oscillator (OPO) comprising a type II nonlinear optical medium for generating a pair of degenerate waves at twice a pump wavelength and a plurality of mirrors for rotating the polarization of one wave by 90 degrees to produce a wavelength-doubled beam with an increased output energy by coupling both of the degenerate waves out of the OPO cavity through the same output coupler following polarization rotation of one of the degenerate waves.

Armstrong, Darrell J. (Albuquerque, NM); Smith, Arlee V. (Albuquerque, NM)

2007-07-24T23:59:59.000Z

209

Double shell tank waste analysis plan  

SciTech Connect (OSTI)

Waste analysis plan for the double shell tanks. SD-WM-EV-053 is Superseding SD-WM-EV-057.This document provides the plan for obtaining information needed for the safe waste handling and storage of waste in the Double Shell Tank Systems. In Particular it addresses analysis necessary to manage waste according to Washington Administrative Code 173-303 and Title 40, parts 264 and 265 of the Code of Federal Regulations.

Mulkey, C.H.; Jones, J.M.

1994-12-15T23:59:59.000Z

210

DNA attachment to support structures  

SciTech Connect (OSTI)

Microscopic beads or other structures are attached to nucleic acids (DNA) using a terminal transferase. The transferase adds labeled dideoxy nucleotide bases to the ends of linear strands of DNA. The labels, such as the antigens digoxigenin and biotin, bind to the antibody compounds or other appropriate complementary ligands, which are bound to the microscopic beads or other support structures. The method does not require the synthesis of a synthetic oligonucleotide probe. The method can be used to tag or label DNA even when the DNA has an unknown sequence, has blunt ends, or is a very large fragment (e.g., >500 kilobase pairs).

Balhorn, Rodney L. (Livermore, CA); Barry, Christopher H. (Fresno, CA)

2002-01-01T23:59:59.000Z

211

A Route to Scale up DNA Origami Using DNA Tiles as Folding Staples  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

A Route to Scale up DNA Origami Using DNA Tiles as Folding Staples Authors: Zhao, Z., Yan, H., and Liu, Y. Title: A Route to Scale up DNA Origami Using DNA Tiles as Folding Staples...

212

A Model for Structure and Thermodynamics of ssDNA and dsDNA Near...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Structure and Thermodynamics of ssDNA and dsDNA Near a Surface:A Coarse Grained Approach. A Model for Structure and Thermodynamics of ssDNA and dsDNA Near a Surface:A Coarse...

213

Design of a New Fluorescent Cofactor for DNA Methyltransferases and Sequence-Specific Labeling of DNA  

Science Journals Connector (OSTI)

Sequence-specific labeling of DNA is of immense interest for analytical and functional studies of DNA. We present a novel approach for sequence-specific labeling of DNA using a newly designed fluorescent cofactor for the DNA methyltransferase from ...

Goran Pljevaljcic; Marc Pignot; Elmar Weinhold

2003-02-26T23:59:59.000Z

214

THE ROLE OF SYNTHETIC DNA IN THE PREPARATION OF STRUCTURAL GENES CODING FOR PROTEINS  

Science Journals Connector (OSTI)

Publisher Summary This chapter discusses the role of synthetic DNA in the preparation of structural genes coding for proteins. One of the key steps in the production of proteins by recombinant DNA technology is the preparation of structural genes coding for the desired proteins. There may be two approaches to this goal: (1) artificial gene synthesis and (2) a combination of cloned cDNA sequences and synthetic DNAs. The method of artificial gene synthesis for the preparation of artificial genes was developed for the synthesis of genes for tRNAs in the late 1960s. The approach of combination of cloned cDNA sequences and synthetic DNA has been developed for the preparation of a gene coding for the human growth hormone. The chapter describes an alternative approach for the synthesis of polydeoxyribonucleotides with defined sequences longer than those accessible by conventional chemical methods. Recent advances in the chemical synthesis of oligonucleotides help to synthesize single-stranded oligonucleotides of up to 40 bases in length, rapidly. It is now possible to synthesize double-stranded DNA fragments using DNA polymerase I in the presence of four deoxynucleoside triphosphosphates.

John J. Rossi; Ryszard Kierzek; Ting Huang; Peter Walker; Keiichi Itakura

1982-01-01T23:59:59.000Z

215

Computer code for double beta decay QRPA based calculations  

E-Print Network [OSTI]

. The Enriched Xenon Observatory for neutrinoless double beta decay (EXO) will search for the rare decays

Bertulani, Carlos A. - Department of Physics and Astronomy, Texas A&M University

216

DNA Meta-Molecules: Synthe4c Biology via DNA Nanostructures &  

E-Print Network [OSTI]

reaction - DNA walkers - Activatable tiles and assemblies (4) DNA-based synthetic biology (slides 107-122) - Synthetic biology - DNA-based meta molecules and their use in synthetic biology - Meta DNA #12;SelfDNA Meta-Molecules: Synthe4c Biology via DNA Nanostructures & Hybridiza4on Reac

Reif, John H.

217

Integrated Microfluidic Electrochemical DNA Sensor  

E-Print Network [OSTI]

of small scale fluid flow Laminar flow Easy to predict the flow patterns Very little diffusion This can make mixing difficult Small volumes Don't need to waste expensive reagents Easy fluid control;DNA Purification The DNA will be extracted using Invitrogen Charge Switch beads. Cellular Lysis

Fygenson, Deborah Kuchnir

218

Single Molecule Studies of Telomere DNA  

E-Print Network [OSTI]

2009) G-quadruplex DNA bound by a synthetic ligand is highly2009) G-quadruplex DNA bound by a synthetic ligand is highly2009) G-quadruplex DNA bound by a synthetic ligand is highly

Long, Xi

2014-01-01T23:59:59.000Z

219

DNA Origami: A History and Current Perspective  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

DNA origami' has emerged as one of the most promising assembly techniques in DNA nanotechnology with a broad range of applications. In the past two years alone, DNA origami has...

220

The BLM Helicase Is Necessary for Normal DNA Double-Strand Break Repair  

Science Journals Connector (OSTI)

...control, corrected BS, BS, and AT cells in a DSB...EcoRI-cut CIP pUC18 in nuclear extracts are shown. Part...DSB repair assays. The costs of publication of this...abbreviations used are: BS, Blooms syndrome; AT...of linearized supF20 by nuclear extracts from control...

Gregory Langland; James Elliott; Yuling Li; Jenette Creaney; Kathleen Dixon; and Joanna Groden

2002-05-15T23:59:59.000Z

Note: This page contains sample records for the topic "dna double helix" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


221

Induction of linear tracks of DNA double-strand breaks by -particle irradiation of  

E-Print Network [OSTI]

eukaryotic cells by exposing the cells to particles from a small Americium source (Box 1). Each particle) EQUIPMENT Americium (Am-241) source (SupplementaryMethods and SupplementaryFigs.1Ā­3 online) Polyester film

Cai, Long

222

A MICROBEAM STUDY OF DNA DOUBLE-STRAND BREAKS IN BYSTANDER PRIMARY HUMAN FIBROBLASTS  

E-Print Network [OSTI]

-or-nothing manner(7) . Bystander cells exhibit a variety of characteristics of IR-induced genomic instability

223

Genetics of x-ray induced double strand break repair in saccharomyces cerevisiae  

SciTech Connect (OSTI)

The possible fates of x-ray-induced double-strand breaks in Saccharomyces cerevisiae were examined. One possible pathway which breaks can follow, the repair pathway, was studied by assaying strains with mutations in the RAD51, RAD54, and RAD57 loci for double-strand break repair. In order of increasing radiation sensitivity one finds: rad57-1(23/sup 0/)> rad51-1(30/sup 0/)> rad54-3(36/sup 0/). At 36/sup 0/, rad54-3 cells cannot repair double-strand breaks, while 23/sup 0/, they can. Strains with the rad57-1 mutation can rejoin broken chromosomes at both temperatures. However, the low survival at 36/sup 0/ shows that the assay is not distinguishing large DNA fragments which allow cell survival from those which cause cell death. A rad51-1 strain could also rejoin broken chromosomes, and was thus capable of incomplete repair. The data can be explained with the hypothesis that rad54-3 cells are blocked in an early step of repair, while rad51-1 and rad57-1 strains are blocked in a later step of repair. The fate of double-strand breaks when they are left unrepaired was investigated with the rad54-3 mutation. If breaks are prevented from entering the RAD54 repair pathway they become uncommitted lesions. These lesions are repaired slower than the original breaks. One possible fate for an uncommitted lesion is conversion into a fixed lesion, which is likely to be an unrepairable or misrepaired double-strand break. The presence of protein synthesis after irradiation increases the probability that a break will enter the repair pathway. Evidence shows that increased probability of repair results from enhanced synthesis of repair proteins shortly after radiation. (ERB)

Budd, M.E.

1982-07-01T23:59:59.000Z

224

Precision Muon Reconstruction in Double Chooz  

E-Print Network [OSTI]

We describe a muon track reconstruction algorithm for the reactor anti-neutrino experiment Double Chooz. The Double Chooz detector consists of two optically isolated volumes of liquid scintillator viewed by PMTs, and an Outer Veto above these made of crossed scintillator strips. Muons are reconstructed by their Outer Veto hit positions along with timing information from the other two detector volumes. All muons are fit under the hypothesis that they are through-going and ultrarelativistic. If the energy depositions suggest that the muon may have stopped, the reconstruction fits also for this hypothesis and chooses between the two via the relative goodness-of-fit. In the ideal case of a through-going muon intersecting the center of the detector, the resolution is ~40 mm in each transverse dimension. High quality muon reconstruction is an important tool for reducing the impact of the cosmogenic isotope background in Double Chooz.

Abe, Y; Barriere, J C; Baussan, E; Bekman, I; Bergevin, M; Bezerra, T J C; Bezrukov, L; Blucher, E; Buck, C; Busenitz, J; Cabrera, A; Caden, E; Camilleri, L; Carr, R; Cerrada, M; Chang, P -J; Chauveau, E; Chimenti, P; Collin, A P; Conover, E; Conrad, J M; Crespo-Anadón, J I; Crum, K; Cucoanes, A; Damon, E; Dawson, J V; Dietrich, D; Djurcic, Z; Dracos, M; Elnimr, M; Etenko, A; Fallot, M; von Feilitzsch, F; Felde, J; Fernandes, S M; Fischer, V; Franco, D; Franke, M; Furuta, H; Gil-Botella, I; Giot, L; Göger-Neff, M; Gonzalez, L F G; Goodenough, L; Goodman, M C; Grant, C; Haag, N; Hara, T; Haser, J; Hofmann, M; Horton-Smith, G A; Hourlier, A; Ishitsuka, M; Jochum, J; Jollet, C; Kaether, F; Kalousis, L N; Kamyshkov, Y; Kaplan, D M; Kawasaki, T; Kemp, E; de Kerret, H; Kryn, D; Kuze, M; Lachenmaier, T; Lane, C E; Lasserre, T; Letourneau, A; Lhuillier, D; Lima, H P; Lindner, M; no, J M López-Casta; LoSecco, J M; Lubsandorzhiev, B; Lucht, S; Maeda, J; Mariani, C; Maricic, J; Martino, J; Matsubara, T; Mention, G; Meregaglia, A; Miletic, T; Milincic, R; Minotti, A; Nagasaka, Y; Nikitenko, Y; Novella, P; Obolensky, M; Oberauer, L; Onillon, A; Osborn, A; Palomares, C; Pepe, I M; Perasso, S; Pfahler, P; Porta, A; Pronost, G; Reichenbacher, J; Reinhold, B; Röhling, M; Roncin, R; Roth, S; Rybolt, B; Sakamoto, Y; Santorelli, R; Schilithz, A C; Schönert, S; Schoppmann, S; Shaevitz, M H; Sharankova, R; Shimojima, S; Sibille, V; Sinev, V; Skorokhvatov, M; Smith, E; Spitz, J; Stahl, A; Stancu, I; Stokes, L F F; Strait, M; Stüken, A; Suekane, F; Sukhotin, S; Sumiyoshi, T; Sun, Y; Svoboda, R; Terao, K; Tonazzo, A; Thi, H H Trinh; Valdiviesso, G; Vassilopoulos, N; Veyssiere, C; Vivier, M; Wagner, S; Watanabe, H; Wiebusch, C; Winslow, L; Wurm, M; Yang, G; Yermia, F; Zimmer, V

2014-01-01T23:59:59.000Z

225

The double-arm barn door tracker  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

How to build a double-arm barn door tracker How to build a double-arm barn door tracker | Jefferson Lab Home Page | Science Education Home Page | Construction Notes/Photos Page | Sources: Sky & Telescope April 1989 (p436 - p441) [very good] Sky & Telescope February 1988 (p213 - p214) Original concept by Dave Trott A single-arm barn door tracker, driven by a straight screw, accumulates tangent error as time passes. Most of this error can be eliminated by adding a second hinged arm to the standard arrangement. There are four types of double-arm trackers, each with a different geometry. A comparison of accumulated error (in arc seconds) and construction parameters is given below: Error Chart [Apparently Type 1 is very bad and not worth constructing?? The two Type 4 drives vary in beta. This results in shifting the region of maximum error

226

Challenges and opportunities for structural DNA nanotechnology  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Challenges and opportunities for structural DNA nanotechnology Authors: Pinheiro, A. V., Han, D., Shih, W. M., and Yan, H. Title: Challenges and opportunities for structural DNA...

227

On the neutrinoless double ?{sup +}/EC decays  

SciTech Connect (OSTI)

The neutrinoless double positron-emission/electron-capture (0??{sup +}/EC) decays are studied for the magnitudes of the involved nuclear matrix elements (NMEs). Decays to the ground state, 0{sub gs}{sup +}, and excited 0{sup +} states are discussed. The participant many-body wave functions are evaluated in the framework of the quasiparticle random-phase approximation (QRPA). Effective, G-matrix-derived nuclear forces are used in realistic single-particle model spaces. The channels ?{sup +}?{sup +}, ?{sup +}EC, and the resonant neutrinoless double electron capture (R0?ECEC) are discussed.

Suhonen, Jouni [Department of Physics, P.O. Box 35 (YFL), FI-40014 University of Jyväskylä (Finland)

2013-12-30T23:59:59.000Z

228

Nanotechnology Applications in Self-Assembly and DNA Computing  

E-Print Network [OSTI]

Covalent attachment of synthetic DNA to self-assembledof synthetic oligodeoxyribonucleotides to phi chi 174 DNA:

Akin, Hayri Engin

2011-01-01T23:59:59.000Z

229

Sequence independent amplification of DNA  

DOE Patents [OSTI]

The present invention is a rapid sequence-independent amplification procedure (SIA). Even minute amounts of DNA from various sources can be amplified independent of any sequence requirements of the DNA or any a priori knowledge of any sequence characteristics of the DNA to be amplified. This method allows, for example, the sequence independent amplification of microdissected chromosomal material and the reliable construction of high quality fluorescent in situ hybridization (FISH) probes from YACs or from other sources. These probes can be used to localize YACs on metaphase chromosomes but also--with high efficiency--in interphase nuclei. 25 figs.

Bohlander, S.K.

1998-03-24T23:59:59.000Z

230

Sequence independent amplification of DNA  

DOE Patents [OSTI]

The present invention is a rapid sequence-independent amplification procedure (SIA). Even minute amounts of DNA from various sources can be amplified independent of any sequence requirements of the DNA or any a priori knowledge of any sequence characteristics of the DNA to be amplified. This method allows, for example the sequence independent amplification of microdissected chromosomal material and the reliable construction of high quality fluorescent in situ hybridization (FISH) probes from YACs or from other sources. These probes can be used to localize YACs on metaphase chromosomes but also--with high efficiency--in interphase nuclei.

Bohlander, Stefan K. (Chicago, IL)

1998-01-01T23:59:59.000Z

231

Unnatural nucleotides for DNA sequencing  

E-Print Network [OSTI]

'-dT4XT5 (below). . . 65 4. 4 Attempted incorporation of dXTP by A) Sequenase~ Version 2. 0, B) Klenow, Exonuclease-free and C) DNA Polymerase, Klenow Fragment. . . . . . . , . . . . . . . . . . . . . . . . . . , . . . . . . 68 4. 5 Attempted... detection of small amounts of DNA present in DNA sequencing gels. Second, tMferent fluorophores are used for each of the base specific reactions. Fluorophores (Figure 1. 4) are covalently attached to the 5' end CHs CHs 0 0 0 HsC CHs 0 ~NH 0 I NH CHs...

Jacutin, Swanee E

2012-06-07T23:59:59.000Z

232

DNA UPTAKE BY TRANSFORMABLE BACTERIA  

SciTech Connect (OSTI)

The various processes of DNA uptake by cells can be categorized as: viral DNA entry, conjugation, or transformation. Within each category, a variety of mechanisms have been found. However, considerable similarities occur among the different mechanisms of conjugation and, especially, transformation. All of these natural mechanisms of DNA transfer are quite elaborate and involve multiple protein components, as the case may be, of the virus, the donor cell, and the recipient cell. The mechanisms of viral infection and conjugation will be discussed mainly with respect to their relevance to transformation.

LACKS,S.A.

1999-09-07T23:59:59.000Z

233

Normalized cDNA libraries  

DOE Patents [OSTI]

This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3' noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library.

Soares, Marcelo B. (New York, NY); Efstratiadis, Argiris (Englewood, NJ)

1997-01-01T23:59:59.000Z

234

Normalized cDNA libraries  

DOE Patents [OSTI]

This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3{prime} noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library. 4 figs.

Soares, M.B.; Efstratiadis, A.

1997-06-10T23:59:59.000Z

235

A Microscopic Double-Slit Experiment  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

A Microscopic Double-Slit A Microscopic Double-Slit Experiment A Microscopic Double-Slit Experiment Print Wednesday, 29 February 2012 00:00 Two centuries ago, Thomas Young performed the classic demonstration of the wave nature of light. He placed a screen with two tiny slits in front of a single light source, effectively converting it into a two-centered source. On a second screen far away, he saw a pattern of light and dark diffraction fringes, a well-known hallmark of wave interference. Along with later studies using particles instead of light, the experiment played a crucial role in establishing the validity of wave-particle duality, a puzzling concept that has ultimately become central to the interpretation of complementarity in quantum mechnanics. In a new twist on this classic experiment, the double slit (with light waves) has been replaced by a diatomic molecule (with electron waves). At ALS Beamline 10.0.1, researchers have shown that diatomic molecules can serve as two-center emitters of electron waves and that traces of electron-wave interference can be directly observed in precise measurements of vibrationally resolved photoionization spectra.

236

Parameterized Algorithms for Double Hypergraph Dualization with  

E-Print Network [OSTI]

as Sperner families.) The support of a hypergraph is the set of all vertices covered by edges, the remaining sets in graphs are also known as vertex covers. We consider the following two operators on hypergraphsParameterized Algorithms for Double Hypergraph Dualization with Rank Limitation and Maximum Minimal

Damaschke, Peter

237

Double tracks test site characterization report  

SciTech Connect (OSTI)

This report presents the results of site characterization activities performed at the Double Tracks Test Site, located on Range 71 North, of the Nellis Air Force Range (NAFR) in southern Nevada. Site characterization activities included reviewing historical data from the Double Tracks experiment, previous site investigation efforts, and recent site characterization data. The most recent site characterization activities were conducted in support of an interim corrective action to remediate the Double Tracks Test Site to an acceptable risk to human health and the environment. Site characterization was performed using a phased approach. First, previously collected data and historical records sere compiled and reviewed. Generalized scopes of work were then prepared to fill known data gaps. Field activities were conducted and the collected data were then reviewed to determine whether data gaps were filled and whether other areas needed to be investigated. Additional field efforts were then conducted, as required, to adequately characterize the site. Characterization of the Double Tracks Test Site was conducted in accordance with the US Department of Energy`s (DOE) Streamlined Approach for Environmental Restoration (SAFER).

NONE

1996-05-01T23:59:59.000Z

238

Apparent Double SiO Maser Sources  

Science Journals Connector (OSTI)

......to 3 Jy with four 20-m antennas (Miyoshi 1998, a poster paper at JAS meeting). The double SiO sources found...Research (C) (No. 10640238) of the Ministry of Education, Science, Sports and Culture. One of authors (SD) received......

Shuji Deguchi; Takahiro Fujii; Hideyuki Izumiura; Shigeru Matsumoto; Yoshikazu Nakada; Peter R. Wood; Issei Yamamura

1999-06-01T23:59:59.000Z

239

Fatigue analysis for double hull VLCC  

SciTech Connect (OSTI)

This paper presents the procedure and the results of fatigue analysis forming a part of Dynamic Load Approach (DLA) applied for 280,000 DWT double hull VLCC, classed with DLA notation by American Bureau of Shipping. The spectral fatigue analysis is applied to the above vessel, and the fatigue strength is confirmed to be sufficient.

Kawachi, S.; Kushima, T.; Negayama, H.; Shigematsu, K. [Sumitomo Heavy Industries, Ltd., Yokosuka (Japan)

1994-12-31T23:59:59.000Z

240

Pion double charge exchange and hadron dynamics  

SciTech Connect (OSTI)

This paper will review theoretical results to show how pion double charge exchange is contributing to our understanding of hadron dynamics in nuclei. The exploitation of the nucleus as a filter is shown to be essential in facilitating the comparison between theory and experiment. 23 refs., 3 figs., 2 tabs.

Johnson, M.B.

1991-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "dna double helix" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


241

Neutrinoless Double $?$-Decay: Status and Future  

E-Print Network [OSTI]

A brief summary of the status of neutrino masses, mixing and oscillations is presented. Neutrinoless double $\\beta$-decay is considered. Predictions for the effective Majorana mass are reviewed. A possible test of the calculations of nuclear matrix elements of the $0\

S. M. Bilenky

2005-09-11T23:59:59.000Z

242

Double Tracks revegetation and monitoring plan  

SciTech Connect (OSTI)

This document is a reclamation plan for short-term and long-term stabilization of land disturbed by activities associated with interim clean-up of radionuclide-contaminated surface soil at the Double Tracks site. This document has been prepared to provide general reclamation practices and procedures that will be followed during restoration of the cleanup site. Reclamation demonstration plots were established near the site in the fall of 1994 to evaluate the performance of several native species and to evaluate different irrigation strategies. Results of the study at Double Tracks, as well as the results from numerous studies conducted at other sites (Area 11 and Area 19 of the Nevada Test Site), have been summarized and incorporated into this final reclamation plan for the interim cleanup of the Double Tracks site, located northwest of the Nevada Test Site on the Nellis Air Force Range. Surface soils at Double Tracks were contaminated as a result of the detonation of a device containing plutonium and depleted uranium using chemical explosives. The total amount of Pu deposited on the site was between 980 and 1,600 grams and was scattered downwind south of the detonation site. Short-term stabilization consists of the application of a chemical soil stabilizer that is applied immediately following excavation of the contaminated soils to minimize Pu resuspension. Long-term stabilization is accomplished by the establishment of a permanent vegetation.

NONE

1997-07-01T23:59:59.000Z

243

Neutrinoless Double Beta Decay and ??Mass Determination  

Science Journals Connector (OSTI)

The search for Neutrinoless Double Beta Decay could improve our knowledge on neutrino properties. After a brief discussion on the implications of the observation of this rare process I will introduce the experimental approaches and review the prospects of the search for this nuclear transition.

M. Pedretti

2005-01-01T23:59:59.000Z

244

Backbone-induced effects in the charge transport efficiency of synthetic DNA  

Science Journals Connector (OSTI)

We report on a theoretical study pointing out the fundamental role of the backbone energetics in the charge transfer efficiency of polyG–polyC and polyA–polyT chains. The double-strand DNA (ds-DNA) molecules are modelled in terms of a single channel effective Hamiltonian. By introducing a two-step renormalization scheme analytical results for the energy spectrum and transmission coefficient are derived, and current–voltage characteristics are numerically investigated. Significant modulations of the main I–V features (voltage threshold, current amplitude) are reported and their physical origin is traced back to backbone-induced electronic effects. These results open new perspectives for experimental work aimed at controlling the charge transfer efficiency in nanodevices based on synthetic DNA.

Enrique Maciį; Stephan Roche

2006-01-01T23:59:59.000Z

245

HIV-1 Tat depresses DNA-PK{sub CS} expression and DNA repair, and sensitizes cells to ionizing radiation  

SciTech Connect (OSTI)

Purpose There is accumulating evidence that cancer patients with human immmunodeficiency virus-1/acquired immunodeficency syndrome (HIV-1/AIDS) have more severe tissue reactions and often develop cutaneous toxic effects when subjected to radiotherapy. Here we explored the effects of the HIV-1 Tat protein on cellular responses to ionizing radiation. Methods and Materials Two Tat-expressing cell lines, TT2 and TE671-Tat, were derived from human rhabdomyosarcoma cells by transfecting with the HIV-1 tat gene. Radiosensitivity was determined using colony-forming ability. Gene expression was assessed by cDNA microarray and immunohybridization. The Comet assay and {gamma}-H2AX foci were use to detect DNA double-strand breaks (DSBs) and repair. Radiation-induced cell cycle changes were detected by flow cytometry. Results The radiosensitivity of TT2 and TE671-Tat cells was significantly increased as compared with parental TE671 cells or the control TE671-pCI cells. Tat also increased proliferation activity. The comet assay and {gamma}H2AX foci detection revealed a decreased capacity to repair radiation-induced DNA DSBs in Tat-expressing cells. Microarray assay demonstrated that the DNA repair gene DNA-PKcs, and cell cycle-related genes Cdc20, Cdc25C, KIF2C and CTS1 were downregulated in Tat-expressing cells. Depression of DNA-PKcs in Tat-expressing cells was further confirmed by RT-PCR and immuno-hybridization analysis. Tat-expressing cells exhibited a prolonged S phase arrest after 4 Gy {gamma}-irradiation, and a noticeable delay in the initiation and elimination of radiation-induced G{sub 2}/M arrest as compared with parental cells. In addition, the G{sub 2}/M arrest was incomplete in TT2 cells. Moreover, HIV-1 Tat resulted in a constitutive overexpression of cyclin B1 protein. Conclusion HIV-1 Tat protein sensitizes cells to ionizing radiation via depressing DNA repair and dysregulating cell cycle checkpoints. These observations provide new insight into the increased tissue reactions of AIDS cancer patients to radiotherapy.

Sun Yi [Department of Radiation Toxicology and Oncology, Beijing Institute of Radiation Medicine, Beijing (China); Huang Yuechen [Department of Radiation Toxicology and Oncology, Beijing Institute of Radiation Medicine, Beijing (China); Xu Qinzhi [Department of Radiation Toxicology and Oncology, Beijing Institute of Radiation Medicine, Beijing (China); Wang Huiping [Department of Radiation Toxicology and Oncology, Beijing Institute of Radiation Medicine, Beijing (China); Bai Bei [Department of Radiation Toxicology and Oncology, Beijing Institute of Radiation Medicine, Beijing (China); Sui Jianli [Department of Radiation Toxicology and Oncology, Beijing Institute of Radiation Medicine, Beijing (China); Zhou Pingkun [Department of Radiation Toxicology and Oncology, Beijing Institute of Radiation Medicine, Beijing (China)]. E-mail: zhoupk@nic.bmi.ac.cn

2006-07-01T23:59:59.000Z

246

Modeling DNA Shuffling Fengzhu Sun  

E-Print Network [OSTI]

Modeling DNA Shuffling Fengzhu Sun 1Department of Genetics Emory University School of Medicine property are selected. Irvine et al. (1991) and Sun et al. (1996) studied in vitro evolution not involving

Sun, Fengzhu - Sun, Fengzhu

247

Nanoscale Molecular Transport by Synthetic DNA Machines  

E-Print Network [OSTI]

Nanoscale Molecular Transport by Synthetic DNA Machines Jong-Shik Shin1 and Niles A. Pierce1,2 1 a processive bipedal DNA walker. Powered by externally controlled DNA fuel strands, the walker locomotes with a 5 nm stride by advancing the trailing foot to the lead at each step. On a periodic DNA track

Pierce, Niles A.

248

Designs of Autonomous Unidirectional Walking DNA Devices  

E-Print Network [OSTI]

a route programmably embedded in the under- lying nanostructure Ā­ existing synthetic DNA mechanical

Reif, John H.

249

Ancient DNA: the first three decades  

Science Journals Connector (OSTI)

...Romanov family by DNA analysis. Nat. Genet. 6...Tyler-Smith, C. 1998 Reliability of DNA-based sex tests...W. 2000 Molecular analysis of Neanderthal DNA from...and mitochondrial DNA analysis of a 2000-year-old...high-density picolitre reactors. Nature 437, 376-380...

2015-01-01T23:59:59.000Z

250

Molecular Computing with DNA Self-Assembly  

E-Print Network [OSTI]

Molecular Computing with DNA Self-Assembly Urmi Majumder #12;Self-Assembly in Nature #12;Key to DNA for Molecular Computing with DNA Self-Assembly Compact: Small library of assembly primitives Complex: Capable in Tiling Assembly: vitroation tural DNA self-assembly has powerful echanisms for error correction

Reif, John H.

251

Covalent Structure of the DNA-DNA Interstrand Cross-Link Formed by Reductively Activated FR66979 in Synthetic DNA Duplexes  

Science Journals Connector (OSTI)

Covalent Structure of the DNA-DNA Interstrand Cross-Link Formed by Reductively Activated FR66979 in Synthetic DNA Duplexes ...

Huifang Huang; Tom K. Pratum; Paul B. Hopkins

1994-04-01T23:59:59.000Z

252

DNA-PK inhibition causes a low level of H2AX phosphorylation and homologous recombination repair in Medaka (Oryzias latipes) cells  

SciTech Connect (OSTI)

Highlights: Black-Right-Pointing-Pointer We investigated the effect of DNA-PK inhibition on DSB repair using fish cells. Black-Right-Pointing-Pointer A radiation sensitive mutant RIC1 strain showed a low level of DNA-PK activity. Black-Right-Pointing-Pointer DNA-PK dysfunction leads defects in HR repair and DNA-PKcs autophosphorylation. Black-Right-Pointing-Pointer DNA-PK dysfunction leads a slight increase in the number of 53BP1 foci after DSBs. Black-Right-Pointing-Pointer DNA-PK dysfunction leads an alternative NHEJ that depends on 53BP1. -- Abstract: Nonhomologous end joining (NHEJ) and homologous recombination (HR) are known as DNA double-strand break (DSB) repair pathways. It has been reported that DNA-PK, a member of PI3 kinase family, promotes NHEJ and aberrant DNA-PK causes NHEJ deficiency. However, in this study, we demonstrate that a wild-type cell line treated with DNA-PK inhibitor and a mutant cell line with dysfunctional DNA-PK showed decreased HR efficiency in fish cells (Medaka, Oryzias latipes). Previously, we reported that the radiation-sensitive mutant RIC1 strain has a defect in the Histone H2AX phosphorylation after {gamma}-irradiation. Here, we showed that a DNA-PK inhibitor, NU7026, treatment resulted in significant reduction in the number of {gamma}H2AX foci after {gamma}-irradiation in wild-type cells, but had no significant effect in RIC1 cells. In addition, RIC1 cells showed significantly lower levels of DNA-PK kinase activity compared with wild-type cells. We investigated NHEJ and HR efficiency after induction of DSBs. Wild-type cells treated with NU7026 and RIC1 cells showed decreased HR efficiency. These results indicated that aberrant DNA-PK causes the reduction in the number of {gamma}H2AX foci and HR efficiency in RIC1 cells. We performed phosphorylated DNA-PKcs (Thr2609) and 53BP1 focus assay after {gamma}-irradiation. RIC1 cells showed significant reduction in the number of phosphorylated DNA-PKcs foci and no deference in the number of 53BP1 foci compared with wild-type cells. These results suggest that low level of DNA-PK activity causes aberrant DNA-PKcs autophosphorylation in RIC1 cells. It is known that 53BP1 is involved in both DNA-PK dependent and independent NHEJ. Therefore we suggest that DNA-PK independent NHEJ repair DSBs under the condition of decreased DNA-PK activity, which causes reduction of HR efficiency.

Urushihara, Yusuke [Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba 277-8562 (Japan)] [Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba 277-8562 (Japan); Kobayashi, Junya [Department of Genome Repair Dynamics, Radiation Biology Center, Kyoto University, Kyoto 606-8501 (Japan)] [Department of Genome Repair Dynamics, Radiation Biology Center, Kyoto University, Kyoto 606-8501 (Japan); Matsumoto, Yoshihisa [Research Laboratory for Nuclear Reactors, Tokyo Institute of Technology, Tokyo 152-8550 (Japan)] [Research Laboratory for Nuclear Reactors, Tokyo Institute of Technology, Tokyo 152-8550 (Japan); Komatsu, Kenshi [Department of Genome Repair Dynamics, Radiation Biology Center, Kyoto University, Kyoto 606-8501 (Japan)] [Department of Genome Repair Dynamics, Radiation Biology Center, Kyoto University, Kyoto 606-8501 (Japan); Oda, Shoji [Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba 277-8562 (Japan)] [Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba 277-8562 (Japan); Mitani, Hiroshi, E-mail: mitani@k.u-tokyo.ac.jp [Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba 277-8562 (Japan)] [Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba 277-8562 (Japan)

2012-12-14T23:59:59.000Z

253

Radiation-induced electron migration along DNA  

SciTech Connect (OSTI)

Radiation-induced electron migration along DNA is a mechanism by which randomly produced stochastic energy deposition events can lead to nonrandom types of damage along DNA manifested distal to the sites of the initial energy deposition. Electron migration along DNA is significantly influenced by the DNA base sequence and DNA conformation. Migration along 7 base pairs in oligonucleotides containing guanine bases was observed for oligonucleotides irradiated in solution which compares to average migration distances of 6 to 10 bases for Escherichia coli DNA irradiated in solution and 5.5 base pairs for Escherichia coli DNA irradiated in cells. Evidence also suggests that electron migration can occur preferentially in the 5{prime} to 3{prime} direction along DNA. Our continued efforts will provide information regarding the contribution of electron transfer along DNA to formation of locally multiply damaged sites created in DNA by exposure to ionizing radiation.

Fuciarelli, A.F.; Sisk, E.C.; Miller, J.H. [Pacific Northwest Lab., Richland, WA (United States); Zimbrick, J.D. [National Research Council, Washington, DC (United States)

1994-04-01T23:59:59.000Z

254

Meta-DNA: synthetic biology via DNA nanostructures and hybridization reactions  

Science Journals Connector (OSTI)

...articles 1004 181 131 18 Meta-DNA: synthetic biology via DNA nanostructures...protocols for its manipulation. Synthetic DNA is also cheaply and readily available...specific to mDNA and also occurs in synthetic DNA synthesis. One of the key technological...

2012-01-01T23:59:59.000Z

255

CONSULTATION RESPONSE The Forensic Use of DNA and the National DNA Database  

E-Print Network [OSTI]

CONSULTATION RESPONSE The Forensic Use of DNA and the National DNA Database Wellcome Trust response on the important topic of the forensic use of DNA and the National DNA Database (NDNAD). Given the Trust Assembly; "Forensic DNA Databasing: A European perspective" - a biomedical ethics grant to Professor Robin

Rambaut, Andrew

256

Monosporascus root rot/vine decline: a study of double-stranded (DS) RNA and its role in the pathogenesis of Monosporascus cannonballus on muskmelon  

E-Print Network [OSTI]

Monosporascus Root Rot/Vine Decline: A Study of Double-Stranded (ds) RNA and Its Role in the Pathogenesis of Monosporascus cannonballus on Muskmelon. (May 1997) Jeffrey Samuel Batten, B. A. , Trinity University Co-Chairs of Advisory Committee: Dr. Raymond D... hybridization between cDNA clones (pJB14 and pJB38) and dsRNA from M 96+ cannonballus isolate, Ca91-17 55 IV-2 Agarose gel and corresponding northern blot showing hybridization between cDNA clones (pJB27, pJB28, pJB39, and pJB40) and dsRNA from M...

Batten, Jeffrey Samuel

2012-06-07T23:59:59.000Z

257

Hanford Begins New Campaign to Remove Excess Water from Double...  

Energy Savers [EERE]

Hanford Begins New Campaign to Remove Excess Water from Double-Shell Tanks Hanford Begins New Campaign to Remove Excess Water from Double-Shell Tanks September 30, 2014 - 12:00pm...

258

Progress Towards Doubling the Beam Power at Fermilab's Accelerator Complex  

SciTech Connect (OSTI)

After a 14 month shutdown accelerator modifications and upgrades are in place to allow us doubling of the Main Injector beam power. We will discuss the past MI high power operation and the current progress towards doubling the power.

Kourbanis, ioanis

2014-06-01T23:59:59.000Z

259

Sensitivity of CUORE to Neutrinoless Double-Beta Decay  

E-Print Network [OSTI]

Sensitivity of CUORE to Neutrinoless Double-Beta Decay b c,:Results of the Search for Neutrinoless D o u b l e - B e t ah e i n a , Evidence for neutrinoless double beta decay, M o

Alessandria, F.

2013-01-01T23:59:59.000Z

260

Double photoionization of helium with synchrotron x-rays: Proceedings  

SciTech Connect (OSTI)

This report contains papers on the following topics: Overview and comparison of photoionization with charged particle impact; The ratio of double to single ionization of helium: the relationship of photon and bare charged particle impact ionization; Double photoionization of helium at high energies; Compton scattering of photons from electrons bound in light elements; Electron ionization and the Compton effect in double ionization of helium; Elimination of two atomic electrons by a single energy photon; Double photoionization of helium at intermediate energies; Double Photoionization: Gauge Dependence, Coulomb Explosion; Single and Double Ionization by high energy photon impact; The effect of Compton Scattering on the double to single ionization ratio in helium; and Double ionization of He by photoionization and Compton scattering. These papers have been cataloged separately for the database.

Not Available

1994-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "dna double helix" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


261

Cellular responses to environmental DNA damage  

SciTech Connect (OSTI)

This volume contains the proceedings of the conference entitled Cellular Responses to Environmental DNA Damage held in Banff,Alberta December 1--6, 1991. The conference addresses various aspects of DNA repair in sessions titled DNA repair; Basic Mechanisms; Lesions; Systems; Inducible Responses; Mutagenesis; Human Population Response Heterogeneity; Intragenomic DNA Repair Heterogeneity; DNA Repair Gene Cloning; Aging; Human Genetic Disease; and Carcinogenesis. Individual papers are represented as abstracts of about one page in length.

Not Available

1994-08-01T23:59:59.000Z

262

EXPRESSION, PURIFICATION, AND SMALL ANGLE X-RAY SCATTERING OF DNA REPLICATION AND REPAIR PROTEINS FROM THE HYPERTHERMOPHILE SULFOLOBUS SOLFATARICUS  

SciTech Connect (OSTI)

Vital molecular processes such as DNA replication, transcription, translation, and maintenance occur through transient protein interactions. Elucidating the mechanisms by which these protein complexes and interactions function could lead to treatments for diseases related to DNA damage and cell division control. In the recent decades since its introduction as a third domain, Archaea have shown to be simpler models for complicated eukaryotic processes such as DNA replication, repair, transcription, and translation. Sulfolobus solfataricus is one such model organism. A hyperthermophile with an optimal growth temperature of 80°C, Sulfolobus protein-protein complexes and transient protein interactions should be more stable at moderate temperatures, providing a means to isolate and study their structure and function. Here we provide the initial steps towards characterizing three DNA-related Sulfolobus proteins with small angle X-ray scattering (SAXS): Sso0257, a cell division control and origin recognition complex homolog, Sso0768, the small subunit of the replication factor C, and Sso3167, a Mut-T like protein. SAXS analysis was performed at multiple concentrations for both short and long exposure times. The Sso0257 sample was determined to be either a mixture of monomeric and dimeric states or a population of dynamic monomers in various conformational states in solution, consistent with a fl exible winged helix domain. Sso0768 was found to be a complex mixture of multimeric states in solution. Finally, molecular envelope reconstruction from SAXS data for Sso3167 revealed a novel structural component which may function as a disordered to ordered region in the presence of its substrates and/or protein partners.

Patterson, S.M.; Hatherill, J.R.; Hammel, M.; Hura, G.L.; Tainer, J.A.; Yannone, S.M.

2008-01-01T23:59:59.000Z

263

/ Search for Neutrinoless Double Beta Decay with CUORE  

E-Print Network [OSTI]

neutrinoless double beta decay (??0?) in 130 Te and other rare processes. The observation of ??0? would

Elena Ferri

264

Geothermal: Sponsored by OSTI -- Structure of the Electric Double...  

Office of Scientific and Technical Information (OSTI)

Structure of the Electric Double Layer in Hydrothermal Systems. Molecular Simulation Approach and Interpretation of Experimental Results...

265

The Majorana Neutrinoless Double-Beta Decay Experiment  

E-Print Network [OSTI]

The Majorana Neutrinoless Double-Beta Decay Experiment Pre-conceptual Design Proposal November 22 Motivation for Neutrinoless Double-Beta Decay Experiments . . . . . . . . . 4 2.1.1 Community Guidance Neutrinoless Double-Beta Decay Results . . . . . . . . . . . . . . . . . . . . 12 2.5 Next

Washington at Seattle, University of - Department of Physics, Electroweak Interaction Research Group

266

Double K-Vacancy Production by X-Ray Photoionization  

E-Print Network [OSTI]

Double K-Vacancy Production by X-Ray Photoionization S. H. SouthworthĀ£ , R. W. DunfordĀ£ , E. P Photon Source. Double K-vacancy production in Ne was observed by recording the KK-KLL Auger. For Mo, double K-vacancy production was observed by recording the K fluorescence hypersatellite

267

Sensitivity of CUORE to Neutrinoless Double-Beta Decay  

E-Print Network [OSTI]

of CUORE to Neutrinoless Double-Beta Decay b c,:L d e f F .s t results on neutrinoless double beta decay of T e w i t hthe study of neutrinoless double beta decay, J . C r y s t .

Alessandria, F.

2013-01-01T23:59:59.000Z

268

Search for Neutrinoless Double-Beta Decay  

E-Print Network [OSTI]

After the pioneering work of the Heidelberg-Moscow (HDM) and International Germanium Experiment (IGEX) groups, the second round of neutrinoless double-$\\beta$ decay searches currently underway has or will improve the life-time limits of double-$\\beta$ decay candidates by a factor of two to three, reaching in the near future the $T_{1/2} = 3 \\times 10^{25}$ yr level. This talk will focus on the large-scale experiments GERDA, EXO-200, and KamLAND-Zen, which have reported already lower half-life time limits in excess of $10^{25}$ yr. Special emphasis is given to KamLAND-Zen, which is expected to approach the inverted hierarchy regime before future 1-ton experiments probe completely this life-time or effective neutrino-mass regime, which starts at $\\approx 2 \\times 10^{26}$ yr or $\\approx 50$ meV.

Tornow, Werner

2014-01-01T23:59:59.000Z

269

Double-clad nuclear fuel safety rod  

DOE Patents [OSTI]

A device for shutting down a nuclear reactor during an undercooling or overpower event, whether or not the reactor's scram system operates properly. This is accomplished by double-clad fuel safety rods positioned at various locations throughout the reactor core, wherein melting of a secondary internal cladding of the rod allows the fuel column therein to shift from the reactor core to place the reactor in a subcritical condition.

McCarthy, William H. (Los Altos, CA); Atcheson, Donald B. (Cupertino, CA); Vaidyanathan, Swaminathan (San Jose, CA)

1984-01-01T23:59:59.000Z

270

A Microscopic Double-Slit Experiment  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

A Microscopic Double-Slit Experiment Print A Microscopic Double-Slit Experiment Print Two centuries ago, Thomas Young performed the classic demonstration of the wave nature of light. He placed a screen with two tiny slits in front of a single light source, effectively converting it into a two-centered source. On a second screen far away, he saw a pattern of light and dark diffraction fringes, a well-known hallmark of wave interference. Along with later studies using particles instead of light, the experiment played a crucial role in establishing the validity of wave-particle duality, a puzzling concept that has ultimately become central to the interpretation of complementarity in quantum mechnanics. In a new twist on this classic experiment, the double slit (with light waves) has been replaced by a diatomic molecule (with electron waves). At ALS Beamline 10.0.1, researchers have shown that diatomic molecules can serve as two-center emitters of electron waves and that traces of electron-wave interference can be directly observed in precise measurements of vibrationally resolved photoionization spectra.

271

A Microscopic Double-Slit Experiment  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

A Microscopic Double-Slit Experiment Print A Microscopic Double-Slit Experiment Print Two centuries ago, Thomas Young performed the classic demonstration of the wave nature of light. He placed a screen with two tiny slits in front of a single light source, effectively converting it into a two-centered source. On a second screen far away, he saw a pattern of light and dark diffraction fringes, a well-known hallmark of wave interference. Along with later studies using particles instead of light, the experiment played a crucial role in establishing the validity of wave-particle duality, a puzzling concept that has ultimately become central to the interpretation of complementarity in quantum mechnanics. In a new twist on this classic experiment, the double slit (with light waves) has been replaced by a diatomic molecule (with electron waves). At ALS Beamline 10.0.1, researchers have shown that diatomic molecules can serve as two-center emitters of electron waves and that traces of electron-wave interference can be directly observed in precise measurements of vibrationally resolved photoionization spectra.

272

Vibration of Generalized Double Well Oscillators  

E-Print Network [OSTI]

We have applied the Melnikov criterion to examine a global homoclinic bifurcation and transition to chaos in a case of a double well dynamical system with a nonlinear fractional damping term and external excitation. The usual double well Duffing potential having a negative square term and positive quartic term has been generalized to a double well potential with a negative square term and a positive one with an arbitrary real exponent $q > 2$. We have also used a fractional damping term with an arbitrary power $p$ applied to velocity which enables one to cover a wide range of realistic damping factors: from dry friction $p \\to 0$ to turbulent resistance phenomena $p=2$. Using perturbation methods we have found a critical forcing amplitude $\\mu_c$ above which the system may behave chaotically. Our results show that the vibrating system is less stable in transition to chaos for smaller $p$ satisfying an exponential scaling low. The critical amplitude $\\mu_c$ as an exponential function of $p$. The analytical results have been illustrated by numerical simulations using standard nonlinear tools such as Poincare maps and the maximal Lyapunov exponent. As usual for chosen system parameters we have identified a chaotic motion above the critical Melnikov amplitude $\\mu_c$.

Grzegorz Litak; Marek Borowiec; Arkadiusz Syta

2006-10-20T23:59:59.000Z

273

Mass Spectrometric Study of Genetic and Epigenetic DNA Modifications  

E-Print Network [OSTI]

of cytosine in synthetic duplex DNA. Moreover, the level ofmethylation in synthetic duplex DNA. The underlined “C”of cytosine in synthetic duplex DNA. Moreover, the global

Wang, Hongxia

2010-01-01T23:59:59.000Z

274

Mass determination of megadalton-DNA Electrospray Ions usingCharge Detection Mass Spectrometry  

SciTech Connect (OSTI)

Charge detection mass spectrometry (CD-MS) has been used to determine the mass of double-stranded, circular DNA and single-stranded, circular DNA in the range of 2500 to 8000 base pairs (1.5-5.0 MDa). Simultaneous measurement of the charge and velocity of an electrostatically accelerated ion allows a mass determination of the ion, with instrument calibration determined independently of samples. Positive ion mass spectra of electrosprayed commercial DNA samples supplied in tris(hydroxymethyl)ethylenediamine tetraacetic acid buffer, diluted in 50 vol. percent acetonitrile, were obtained without cleanup of the sample. ACD mass spectrum constructed from 3000 ion measurements takes 10 min to acquire and yields the DNA molecular mass directly (mass resolution = 6). The data collected represent progress toward a more automatable alternative to sizing of DNA by gel electrophoresis. In addition to the mass spectra, CD-MS generates charge versus mass plots, which provide another means to investigate the creation and fate of large electrospray ions.

Schultz, Jocelyn C.; Hack, Christopher; Benner, Henry W.

1997-10-01T23:59:59.000Z

275

Frequency Doubling Nanocrystals for Cancer Theranostics  

E-Print Network [OSTI]

A novel bio-photonics approach based on the nonlinear optical process of second harmonic generation by non-centrosymmetric nanoparticles is presented and demonstrated on malignant human cell lines. The proposed method allows to directly interact with DNA in absence of photosensitizing molecules, to enable independent imaging and therapeutic modalities switching between the two modes of operation by simply tuning the excitation laser wavelength, and to avoid any risk of spontaneous activation by any natural or artificial light source.

Staedler, Davide; Passemard, Solčne; Schwung, Sebastian; Dubled, Marc; Schneiter, Guillaume Stéphane; Rytz, Daniel; Gerber-Lemaire, Sandrine; Bonacina, Luigi; Wolf, Jean-Pierre

2013-01-01T23:59:59.000Z

276

DNA chips --Integrated Chemical Circuits for DNADiagnosis and DNA computers  

E-Print Network [OSTI]

. Their needs are i nc reasing with the rapid progress of the genome projects. DNA chips will also provide basic about 6,000 genes. In 2002Ā­2005, sequencing of the whole human genome w i l l be finished. There are about 100,000 genes in the human genome. These efforts are about to open a new age where everything

Hagiya, Masami

277

Nuclear matrix elements for neutrinoless double-beta decay and double-electron capture  

E-Print Network [OSTI]

A new generation of neutrinoless double beta decay experiments with improved sensitivity is currently under design and construction. They will probe inverted hierarchy region of the neutrino mass pattern. There is also a revived interest to the resonant neutrinoless double-electron capture, which has also a potential to probe lepton number conservation and to investigate the neutrino nature and mass scale. The primary concern are the nuclear matrix elements. Clearly, the accuracy of the determination of the effective Majorana neutrino mass from the measured 0\

Amand Faessler; Vadim Rodin; Fedor Simkovic

2012-06-03T23:59:59.000Z

278

Structure and Mutagenesis of the Parainfluenza Virus 5 Hemagglutinin-Neuraminidase Stalk Domain Reveals a Four-Helix Bundle and the Role of the Stalk in Fusion Promotion  

SciTech Connect (OSTI)

Paramyxovirus entry into cells requires the fusion protein (F) and a receptor binding protein (hemagglutinin-neuraminidase [HN], H, or G). The multifunctional HN protein of some paramyxoviruses, besides functioning as the receptor (sialic acid) binding protein (hemagglutinin activity) and the receptor-destroying protein (neuraminidase activity), enhances F activity, presumably by lowering the activation energy required for F to mediate fusion of viral and cellular membranes. Before or upon receptor binding by the HN globular head, F is believed to interact with the HN stalk. Unfortunately, until recently none of the receptor binding protein crystal structures have shown electron density for the stalk domain. Parainfluenza virus 5 (PIV5) HN exists as a noncovalent dimer-of-dimers on the surface of cells, linked by a single disulfide bond in the stalk. Here we present the crystal structure of the PIV5-HN stalk domain at a resolution of 2.65 {angstrom}, revealing a four-helix bundle (4HB) with an upper (N-terminal) straight region and a lower (C-terminal) supercoiled part. The hydrophobic core residues are a mix of an 11-mer repeat and a 3- to 4-heptad repeat. To functionally characterize the role of the HN stalk in F interactions and fusion, we designed mutants along the PIV5-HN stalk that are N-glycosylated to physically disrupt F-HN interactions. By extensive study of receptor binding, neuraminidase activity, oligomerization, and fusion-promoting functions of the mutant proteins, we found a correlation between the position of the N-glycosylation mutants on the stalk structure and their neuraminidase activities as well as their abilities to promote fusion.

Bose, Sayantan; Welch, Brett D.; Kors, Christopher A.; Yuan, Ping; Jardetzky, Theodore S.; Lamb, Robert A. (NWU); (Stanford-MED)

2014-10-02T23:59:59.000Z

279

The assessment of DNA-synthetic activity  

Science Journals Connector (OSTI)

A method is described by which a numerical value can be assigned to the amount of DNA-synthesis shown graphically by population-histograms obtained ... index appeared to give a reasonable measure of DNA-synthetic

L. A. Coulton; B. Henderson; J. Chayen

1981-01-01T23:59:59.000Z

280

Fralin Life Science Institute DNA Biotechnology Kit  

E-Print Network [OSTI]

Fralin Life Science Institute DNA Biotechnology Kit INFORMATION MANUAL Kristi DeCourcy & Erin Dolan ..................................................................................................... 3 DNA Biotechnology Kit contents), in partnership with the Virginia Biotechnology Association (VaBIO) and the Virginia Manufacturers Association

Virginia Tech

Note: This page contains sample records for the topic "dna double helix" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


281

A DNA tweezer-actuated enzyme nanoreactor  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

A DNA tweezer-actuated enzyme nanoreactor Authors: Liu, M., Fu, J., Hejesen, C., Yang, Y., Woodbury, N.W., Gothelf, K., Liu, Y., and Yan, H. Title: A DNA tweezer-actuated enzyme...

282

DNA Concentration By UV Spectrophotometry Measure Absorption  

E-Print Network [OSTI]

DNA Concentration By UV Spectrophotometry Measure Absorption: 1. Dilute DNA to 0.5 to 50 Āµg 2. Measure absorption at 260 nm (A260). Start by zeroing instrument with TE buffer or dH2O alone

Aris, John P.

283

Towards Privacy Preserving of Forensic DNA Databases  

E-Print Network [OSTI]

Protecting privacy of individuals is critical for forensic genetics. In a kinship/identity testing, related DNA profiles between user's query and the DNA database need to be extracted. However, unrelated profiles cannot be revealed to each other...

Liu, Sanmin

2012-02-14T23:59:59.000Z

284

Neutrinoless Double Beta Decay with Composite Neutrinos.  

E-Print Network [OSTI]

We study in detail the contribution of heavy composite Majorana neutrinos to neutrino-less double beta decay (0???). Our analysis confirms the result of a previous estimate by two of the authors. Excited neutrinos couple to the electroweak gauge bosons through a magnetic type effective Lagrangian. The relevant nuclear matrix element is related to matrix elements available in the literature and current bounds on the half-life of 0??? are converted into bounds on the compositeness scale and/or the heavy neutrino mass. Our bounds are of the same order of magnitude as those available from accelerator experiments.

O. Panella (a; C. Carimalo (b; Y. N. Srivastava (a; A. Widom (c

1997-01-01T23:59:59.000Z

285

Double acting stirling engine phase control  

DOE Patents [OSTI]

A mechanical device for effecting a phase change between the expansion and compression volumes of a double-acting Stirling engine uses helical elements which produce opposite rotation of a pair of crankpins when a control rod is moved, so the phase between two pairs of pistons is changed by +.psi. and the phase between the other two pairs of pistons is changed by -.psi.. The phase can change beyond .psi.=90.degree. at which regenerative braking and then reversal of engine rotation occurs.

Berchowitz, David M. (Scotia, NY)

1983-01-01T23:59:59.000Z

286

Neutrino Oscillations, Neutrinoless Double Beta Decay  

Science Journals Connector (OSTI)

We review the experimental evidence for neutrino mixing and neutrino mass. Searches for possible branches into heavy neutrinos do not reveal evidence for static mixing with branching ratios larger than 10?4 to 10?6. Similarly neutrino oscillation experiments show no evidence for dynamic mixing in various oscillation channels. Stringent limits for ? e disappearance from a recent reactor experiment are presented. Results from neutrinoless double beta decay provide sensitive test for Majorana mass and right?hand couplings the present limits being 3–10 eV and 10?5 respectively.

F. Boehm

1982-01-01T23:59:59.000Z

287

Neutrinoless double beta decay and neutrino masses  

Science Journals Connector (OSTI)

Neutrinoless double beta decay (0???) is a promising test for lepton number violating physics beyond the standard model (SM) of particle physics. There is a deep connection between this decay and the phenomenon of neutrino masses. In particular we will discuss the relation between 0??? and Majorana neutrino masses provided by the so-called Schechter-Valle theorem in a quantitative way. Furthermore we will present an experimental cross check to discriminate 0??? from unknown nuclear background using only one isotope i.e. within one experiment.

Michael Duerr

2012-01-01T23:59:59.000Z

288

Neutrinoless double ? decay with composite neutrinos  

Science Journals Connector (OSTI)

We study in detail the contribution of heavy composite Majorana neutrinos to neutrinoless double beta decay (0???). Our analysis confirms the result of a previous estimate by two of the authors. Excited neutrinos couple to the electroweak gauge bosons through a magnetic-type effective Lagrangian. The relevant nuclear matrix element is related to matrix elements available in the literature and current bounds on the half-life of 0??? are converted into bounds on the compositeness scale and/or the heavy neutrino mass. Our bounds are of the same order of magnitude as those available from accelerator experiments.

O. Panella; C. Carimalo; Y. N. Srivastava; A. Widom

1997-11-01T23:59:59.000Z

289

Chromosome specific repetitive DNA sequences  

DOE Patents [OSTI]

A method is provided for determining specific nucleotide sequences useful in forming a probe which can identify specific chromosomes, preferably through in situ hybridization within the cell itself. In one embodiment, chromosome preferential nucleotide sequences are first determined from a library of recombinant DNA clones having families of repetitive sequences. Library clones are identified with a low homology with a sequence of repetitive DNA families to which the first clones respectively belong and variant sequences are then identified by selecting clones having a pattern of hybridization with genomic DNA dissimilar to the hybridization pattern shown by the respective families. In another embodiment, variant sequences are selected from a sequence of a known repetitive DNA family. The selected variant sequence is classified as chromosome specific, chromosome preferential, or chromosome nonspecific. Sequences which are classified as chromosome preferential are further sequenced and regions are identified having a low homology with other regions of the chromosome preferential sequence or with known sequences of other family me This invention is the result of a contract with the Department of Energy (Contract No. W-7405-ENG-36).

Moyzis, Robert K. (Los Alamos, NM); Meyne, Julianne (Los Alamos, NM)

1991-01-01T23:59:59.000Z

290

International Standards in Forensic DNA  

E-Print Network [OSTI]

International Standards in Forensic DNA John M. Butler, Ph.D. National Institute of Standards and Technology NIST Fellow & Special Assistant to the Director for Forensic Science Vice-Chair, National Commission on Forensic Science World Forensics Festival Seoul, Korea October 15, 2014 #12;Definition

291

DNA Hybridization Catalysts and Catalyst Circuits  

E-Print Network [OSTI]

blocks for a synthetic DNA-based circuit. While ri- bozymes are the best known example of nucleic acids

Winfree, Erik

292

Amplification of chromosomal DNA in situ  

DOE Patents [OSTI]

Amplification of chromosomal DNA in situ to increase the amount of DNA associated with a chromosome or chromosome region is described. The amplification of chromosomal DNA in situ provides for the synthesis of Fluorescence in situ Hybridization (FISH) painting probes from single dissected chromosome fragments, the production of cDNA libraries from low copy mRNAs and improved in Comparative Genomic Hybridization (CGH) procedures.

Christian, Allen T. (Tracy, CA); Coleman, Matthew A. (Livermore, CA); Tucker, James D. (Livermore, CA)

2002-01-01T23:59:59.000Z

293

Mutations & DNA Repair I. What are Mutations?  

E-Print Network [OSTI]

Ā­ one strand loops out and becomes displaced during replication Ā­ DNA pol stuttering Ā­ Occurs frequently

Dever, Jennifer A.

294

Meta-DNA: A DNA-Based Approach to Synthetic Harish Chandran1  

E-Print Network [OSTI]

Meta-DNA: A DNA-Based Approach to Synthetic Biology Harish Chandran1 harish@cs.duke.edu Nikhil Gopalkrishnan, Bernard Yurke, John Reif, Meta-DNA: Synthetic Biology via DNA Nanostructures and Hybridization@cs.duke.edu Abstract The goal of synthetic biology is to design and assemble synthetic systems that mimic bio- logical

Reif, John H.

295

Identification of sequence-dependent DNA features correlating to activity of DNA sites interacting with proteins  

Science Journals Connector (OSTI)

......transcription factor MEF-2C for synthetic DNA (Meierhans et al ., 1997; Meierhans...Savinkova et al ., 1998) Synthetic DNA start 20 affinity yTBP/DNA X1...Bendall and Molloy, 1994) Synthetic DNA start X1 X2 10; 13 10; 18 depth......

MP Ponomarenko; JV Ponomarenko; AS Frolov; NL Podkolodny; LK Savinkova; NA Kolchanov; GC Overton

1999-07-01T23:59:59.000Z

296

DNA nanotubes and helical nanotapes via self-assembly of ssDNA-amphiphiles  

E-Print Network [OSTI]

DNA nanotubes and helical nanotapes via self- assembly of ssDNA-amphiphiles Timothy R. Pearcea and Efrosini Kokkoli*b DNA nanotubes were created using molecular self-assembly of single-stranded DNA (ss. The nanotube structures were formed by bilayers of amphiphiles, with the hydrophobic components forming

Kokkoli, Efie

297

Supplementary Information for: Integrating DNA Strand Displacement Circuitry with DNA Tile Self-assembly  

E-Print Network [OSTI]

Supplementary Information for: Integrating DNA Strand Displacement Circuitry with DNA Tile Self-assembly of Contents: Ā· Supplementary Figures 1. Native polyacrylamide gel electrophoresis of DNA tile self-assembly 2. UV absorbance annealing and melting curves of DNA tile self-assembly 3. Characterization

Zhang, David Yu

298

DNA adsorption on synthetic and natural allophanes  

Science Journals Connector (OSTI)

The adsorption of DNA on natural and synthetic samples of allophane, which is a primary clay mineral of andosols, was investigated with respect to the DNA concentration, pH, ionic strength in the sample solution, and competition between DNA molecules and phosphate ions for adsorption to understand the behavior of extracellular DNA molecules in andosols. The relationships between DNA adsorption and the final concentrations were significantly fitted to a simple linear Langmuir equation. DNA adsorption decreased considerably with increasing suspension pH in the range between 3 and 9. The adsorption was less affected by the ionic strength of the suspension from 0.1 to 0.5 mol L? 1. Under the same experimental conditions, DNA adsorption on allophanes was relatively higher than that on montmorillonite and silica but relatively lower than that on gibbsite and goethite. DNA adsorption on allophanes decreased on the addition of phosphate, indicating that there was a competition between DNA molecules and phosphate ions for adsorption on the minerals. These results suggested that DNA adsorption on allophanes occurred via two mechanisms: direct bonding of the phosphate group at the end of the DNA molecule to the OH groups of the Al-oxide layer on allophane, and the association of DNA molecules with the surface of negatively charged allophane via a bridging of inorganic cations.

Kazutoshi Saeki; Masao Sakai; Shin-Ichiro Wada

2010-01-01T23:59:59.000Z

299

Electromechanical Unzipping of Individual DNA Molecules Using  

E-Print Network [OSTI]

Electromechanical Unzipping of Individual DNA Molecules Using Synthetic Sub-2 nm Pores Ben Mc synthetic pores can be used to electrome- chanically unzip DNA duplexes.13 However, direct measure- ment time that the unzipping kinetics of individual DNA duplexes can be probed by analyzing the dwell

300

DNA Nanotechnology and its Biological Applications1  

E-Print Network [OSTI]

1 DNA Nanotechnology and its Biological Applications1 Chapter 13 of Book: Bio-inspired and Nano, amplified sensing, and molecular transport. 13.1 Introduction 13.1.1 DNA Nanotechnology and its use conventional top-down manufacturing techniques. Other surveys of DNA nanotechnology and devices have been given

Reif, John H.

Note: This page contains sample records for the topic "dna double helix" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


301

Self-assembled DNA Structures for Nanoconstruction  

E-Print Network [OSTI]

Self-assembled DNA Structures for Nanoconstruction Hao Yan, Peng Yin, Sung Ha Park, Hanying Li methods based on DNA self-assembly. Here we review our recent experimental progress to utilize novel DNA nanostructures for self-assembly as well as for templates in the fabrication of functional nano

Yin, Peng

302

HELICASE DEPENDENT AMPLICATION transfer the DNA  

E-Print Network [OSTI]

STEP 1 STEP 2 STEP 3 HELICASE DEPENDENT AMPLICATION HELICASE DNA POLYMERASE transfer the DNA instrumentation. MOLECULAR BIOMARKERS MADE UP DNA or RNA STRANDS ARE A POWERFUL WEAPON IN DETECTING DISEASE. USING for sample preparation at the point to care using only hand generated power.... #12;

303

Photoaffinity Labeling of Mouse Fibroblast Enzymes by a Base Excision Repair Intermediate: New Evidence on the Role of PARP-1 in DNA Repair  

SciTech Connect (OSTI)

To examine mammalian base excision repair (BER) enzymes interacting with DNA intermediates formed during BER, we used a novel photoaffinity labeling probe and mouse embryonic fibroblast (MEF) crude extract. The probe was formed in situ, using an end-labeled oligonucleotide containing a synthetic abasic site; this site was incised by AP endonuclease creating a nick with 3' hydroxyl and 5' reduced sugar phosphate groups at the margins, and then a dNMP carrying a photoreactive adduct was introduced at the 3' hydroxyl group. With near UV-light exposure (312nm) of the extract-probe mixture, only six proteins were strongly labeled, including poly (ADP-ribose) polymerase (PARP-1) and the well-known BER participants flap endonuclease (FEN-1), DNA polymerase b (b-pol), and AP endonuclease (APE). The amount of probe crosslinked to PARP-1 was greater than that crosslinked to the other proteins. The specificity of PARP-1 labeling was examined by competition experiments involving various oligonucleotide competitors; competition of labeling by the probe was much greater for the BER intermediates tested than for normal double-stranded DNA. The specificity of PARP-1 labeling also was examined using DNA probes with alternate structures; PARP-1 labeling was stronger with a DNA oligomer representing a BER intermediate than with a molecule representing a nick in double-stranded DNA. These results identifying interaction of PARP-1 with a BER intermediate are discussed in light of PARP-1's role in mammalian BER.

Lavrik, Olga I.(National Institute of Environmental Health Sciences) [National Institute of Environmental Health Sciences; Prasad, Rajendra (National Institute of Environmental Health Sciences) [National Institute of Environmental Health Sciences; Sobol, Robert W.(National Institute of Environmental Health Sciences) [National Institute of Environmental Health Sciences; Horton, Julie K.(National Institute of Environmental Health Sciences) [National Institute of Environmental Health Sciences; Ackerman, Eric J.(BATTELLE (PACIFIC NW LAB)) [BATTELLE (PACIFIC NW LAB); Wilson, Samuel H.(National Institute of Environmental Health Sciences) [National Institute of Environmental Health Sciences

2001-07-06T23:59:59.000Z

304

Self-Assembled DNA Crystals: The Impact on Resolution of 5?-Phosphates and the DNA Source  

Science Journals Connector (OSTI)

Self-assembled 3D DNA crystals; crystal resolution; phosphorylated DNA; natural and synthetic DNA; X-ray diffraction; designed crystals ... During the last two decades, we have found two instances where synthetic DNA did not produce the results expected from it, when used as an enzymatic substrate; by contrast, DNA generated by the polymerase chain reaction (PCR) was an effective substrate. ... One example was the low level at which a synthetic DNA molecule was transcribed by T7 RNA polymerase to produce an RNA knot;(6, 7) the other instance was the incomplete restriction of a DNA graph assembled from synthetic branched junctions. ...

Ruojie Sha; Jens J. Birktoft; Nam Nguyen; Arun Richard Chandrasekaran; Jianping Zheng; Xinshuai Zhao; Chengde Mao; Nadrian C. Seeman

2013-01-16T23:59:59.000Z

305

Double pion photoproduction off 40Ca  

E-Print Network [OSTI]

The photoproduction of pi0pi0 and pi0pi+/- pairs off 40Ca has been investigated with the TAPS detector using the Glasgow photon tagging spectrometer at the Mainz MAMI accelerator. Data have been taken for incident photon energies in the energy range from 200 - 820 MeV. Total cross sections have been extracted from threshold up to the maximum photon energy and invariant mass distributions of the pion pairs have been obtained for incident photon energies between 400 - 500 MeV and 500 - 550 MeV. The double pi0 invariant mass distributions show some relative enhancement with respect to the mixed charge channel at small invariant masses. The effects are smaller than previously observed for lead nuclei and the distributions do not significantly deviate from carbon data. The data are in good agreement with the results of recent calculations in the framework of the BUU model, with careful treatment of final state interaction effects but without an explicit in-medium modification of scalar, iso-scalar pion pairs. This means that for Ca most of the experimentally observed effect can be explained by final state interactions. Only at low incident photon energies there is still a small low mass enhancement of the double pi0 data over the BUU results.

F. Bloch; J. Ahrens; J. R. M. Annand; R. Beck; L. S. Fog; D. Hornidge; S. Janssen; M. Kotulla; B. Krusche; J. C. McGeorge; I. J. D. MacGregor; J. G. Messchendorp; V. Metag; R. Novotny; R. O. Owens; M. Pfeiffer; M. Rost; R. Sanderson; S. Schadmand; A. Thomas; D. P. Watts

2007-03-24T23:59:59.000Z

306

Search For Neutrinoless Double Beta Decay  

E-Print Network [OSTI]

The HEIDELBERG-MOSCOW experiment, which is the most sensitive double beta decay experiment since ten years has been regularly continued until end of November 2003. An analysis of the data has been performed already until May 20, 2003. The experiment yields now, on a 4? level, evidence for lepton number violation and proves that the neutrino is a Majorana particle. It further shows that neutrino masses are degenerate. In addition it puts several stringent constraints on other physics beyond the Standard Model. Among others it opens the door to test various supersymmetric theory scenarios, for example it gives the sharpest in the R-parity violating part of the superpotential, and limit on the parameter ? ? 111 gives information on the splitting of the sneutrino-antisneutrino system. The result from the HEIDELBERG-MOSCOW experiment is consistent with recent results from CMB investigations, with high energy cosmic rays, with the result from the g-2 experiment and with recent theoretical work. It is indirectly supported by the analysis of other Ge double beta experiments. Recent criticism of various kind has been shown to be wrong, among others by measurements performed in 2003 with a 214Bi source ( 226Ra), by simulation of the background in the range of Q?? by GEANT4, and by deeper investigation of statistical features such as sensitivity of peak search, and relevance of width of window of analysis. 1

H. V. Klapdor-kleingrothaus *a; I. V. Krivosheina A; A. Dietz A

2004-01-01T23:59:59.000Z

307

Particle acceleration in double radio sources  

Science Journals Connector (OSTI)

Further study has been made of a model of extragalactic double radio sources in which the magnetized accretion disc of a massive black hole acts as an electric dynamo producing oppositely directed beams of relativistic particles. An empty region of small angular size—a vortice core—is shown to be a possible consequence of the hydrodynamics of accretion of gas with angular momentum. An ambient magnetic field in the gas tends to accumulate in the core region. The acceleration of electrons in the dynamo electric field gives rise to oppositely directed electron beams which in turn produce synchroton gamma rays collimated along the core. Collisions between the gamma rays and low energy photons caN initiate an electromagnetic cascade shower. The cascade acts to produce a collimated relativistic electron?positron beam. If the current flow in the dynamo occurs in surges then the outward propagation of the head of the electron–positron beams may produce expanding compact radio components. The electron–positron beam is ballistic in nature and it is electrically neutral and carries no current. Such a beam appears to have favorable stability properties. The beam is eventually stopped and spread out in pitch angle by the ram pressure of the external medium. Synchrotron radiated lobes of double radio sources.

R. V. E. Lovelace; J. MacAuslan; M. Burns

1979-01-01T23:59:59.000Z

308

Double Retort System for Materials Compatibility Testing  

SciTech Connect (OSTI)

With Naval Reactors (NR) approval of the Naval Reactors Prime Contractor Team (NRPCT) recommendation to develop a gas cooled reactor directly coupled to a Brayton power conversion system as the Space Nuclear Power Plant (SNPP) for Project Prometheus (References a and b) there was a need to investigate compatibility between the various materials to be used throughout the SNPP. Of particular interest was the transport of interstitial impurities from the nickel-base superalloys, which were leading candidates for most of the piping and turbine components to the refractory metal alloys planned for use in the reactor core. This kind of contamination has the potential to affect the lifetime of the core materials. This letter provides technical information regarding the assembly and operation of a double retort materials compatibility testing system and initial experimental results. The use of a double retort system to test materials compatibility through the transfer of impurities from a source to a sink material is described here. The system has independent temperature control for both materials and is far less complex than closed loops. The system is described in detail and the results of three experiments are presented.

V. Munne; EV Carelli

2006-02-23T23:59:59.000Z

309

DNA–DNA interactions in bacteriophage capsids are responsible for the observed DNA knotting  

Science Journals Connector (OSTI)

...cholesteric liquid crystals. This interaction...polymers confined in a spherical cavity produces, on the...term that disfavors bent-chain configurations...the surface of the spherical capsid (45 nm diameter...cholesteric liquid crystals of DNA . Eur Phys...cholesteric liquid crystals. This interaction...

Davide Marenduzzo; Enzo Orlandini; Andrzej Stasiak; De Witt Sumners; Luca Tubiana; Cristian Micheletti

2009-01-01T23:59:59.000Z

310

Neutrinoless double beta decay search with the NEMO 3 experiment  

Science Journals Connector (OSTI)

The NEMO 3 experiment searches for neutrinoless double beta decay and makes precision measurements of two?neutrino double beta decay in seven isotopes. The latest two?neutrino half?life results are presented together with the limits on neutrinoless half?lives and the corresponding effective Majorana neutrino masses. Also given are the limits obtained on neutrinoless double beta decay mediated by R p ?violating SUSY right?hand currents and different Majoron emission modes.

Irina Nasteva; NEMO collaboration

2008-01-01T23:59:59.000Z

311

The Arabidopsis Basic/Helix-Loop-Helix Transcription Factor Family  

Science Journals Connector (OSTI)

...paramagnetic protein A beads (Dynabeads Protein A Dynal, Oslo, Norway) and GAD antibody (Santa Cruz Biotechnology, Santa Cruz...National Institutes of Health Grant GM47475, Department of Energy Grant DE-FG03-87ER13742, U.S. Department of Agriculture-Agricultural...

Gabriela Toledo-Ortiz; Enamul Huq; Peter H. Quail

2003-07-14T23:59:59.000Z

312

Thermal Performance of a Double-Tube Type Lng Vaporizer  

Science Journals Connector (OSTI)

This report concerns the confirmed test results and method of analysis of the thermal performance of a double-tube type LNG vaporizer (DTV). The DTV is a...

Y. Miyata; T. Miura; S. Kasahara; H. Shohtani…

1994-01-01T23:59:59.000Z

313

Majorana Neutrino Masses from Neutrinoless Double Beta Decay and Cosmology  

E-Print Network [OSTI]

When three Majorana neutrinos describe the solar and atmospheric neutrino data via oscillations, a nonzero measurement of neutrinoless double beta ($0\

V. Barger; K. Whisnant

1999-04-08T23:59:59.000Z

314

Electromagnetically Induced Transparency in a Double Well Atomic Josephson Junction  

E-Print Network [OSTI]

observation of these Josephson junction resonances. 2.dressed Bose condensed Josephson junction Let us consider ain a Double Well Atomic Josephson Junction J.O. Weatherall

Weatherall, J. O.; Search, C. P.

2009-01-01T23:59:59.000Z

315

Neutrinoless double beta decay can constrain neutrino dark matter  

E-Print Network [OSTI]

We examine how constraints can be placed on the neutrino component of dark matter by an accurate measurement of neutrinoless double beta ($0\

V. Barger; S. L. Glashow; D. Marfatia; K. Whisnant

2002-02-26T23:59:59.000Z

316

The complex Doppler effect in double negative media  

Science Journals Connector (OSTI)

The Doppler effect in doubly double negative acoustic media is ... ’s functions. It is shown that several Doppler modes can be generated by a monochromatic...

I. V. Lisenkov; S. A. Nikitov

2011-06-01T23:59:59.000Z

317

A Search for Neutrinoless Double Beta Decay of Te-130.  

E-Print Network [OSTI]

??This dissertation describes an experimental search for neutrinoless double beta (0???) decay of 130Te. An observation of 0??? decay would establish that neutrinos are Majorana… (more)

Bryant, Adam Douglas

2010-01-01T23:59:59.000Z

318

Microfluidic DNA sample preparation method and device  

DOE Patents [OSTI]

Manipulation of DNA molecules in solution has become an essential aspect of genetic analyses used for biomedical assays, the identification of hazardous bacterial agents, and in decoding the human genome. Currently, most of the steps involved in preparing a DNA sample for analysis are performed manually and are time, labor, and equipment intensive. These steps include extraction of the DNA from spores or cells, separation of the DNA from other particles and molecules in the solution (e.g. dust, smoke, cell/spore debris, and proteins), and separation of the DNA itself into strands of specific lengths. Dielectrophoresis (DEP), a phenomenon whereby polarizable particles move in response to a gradient in electric field, can be used to manipulate and separate DNA in an automated fashion, considerably reducing the time and expense involved in DNA analyses, as well as allowing for the miniaturization of DNA analysis instruments. These applications include direct transport of DNA, trapping of DNA to allow for its separation from other particles or molecules in the solution, and the separation of DNA into strands of varying lengths.

Krulevitch, Peter A. (Pleasanton, CA); Miles, Robin R. (Danville, CA); Wang, Xiao-Bo (San Diego, CA); Mariella, Raymond P. (Danville, CA); Gascoyne, Peter R. C. (Bellaire, TX); Balch, Joseph W. (Livermore, CA)

2002-01-01T23:59:59.000Z

319

KUB5/HERA: a dual acting protein that suppresses genomic instability and promotes DNA repair after low dose IR exposure  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

KUB5/HERA: a dual acting protein that suppresses genomic instability and KUB5/HERA: a dual acting protein that suppresses genomic instability and promotes DNA repair after low dose IR exposure Julio C. Morales 1 , Amy Rommel 1 , Konstantin Leskov 2 , Walter M. Hittelman 3 , David A. Boothman 1# 1 Simmons Cancer Center, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA. 2 Department of Radiation Oncology, Case Western Reserve University, Cleveland, OH 44106, USA. 3 Department of Experimental Therapeutics, M.D. Anderson Cancer Center, Houston, TX 77030, USA. # To whom correspondence should be addressed. E-mail: David.Boothman@utsouthwestern.edu Eukaryotic cells can respond to DNA double strand breaks created by low doses of IR by activating homologous recombination (HR) or non-homologous end- joining (NHEJ) pathways to repair DNA. A yeast two-hybrid screen using Ku70 as

320

Human Genome Research: Decoding DNA  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Human Genome Research: Decoding DNA Human Genome Research: Decoding DNA Resources with Additional Information Charles DeLisi As head of DOE's Office of Health and Environmental Research, Charles DeLisi played a pivotal role in proposing and initiating the Human Genome Program in 1986. The U.S. Department of Energy (DOE) has historically been active in supporting human genome research. On September 10, 2003, Secretary of Energy Spencer Abraham presented the Secretary's Gold Award to Aristides Patrinos and Francis Collins for their leadership of the government's Human Genome Project. At DOE's Office of Science, Dr. Patrinos is the Associate Director for Biological and Environmental Research. He has been a researcher at the department's Oak Ridge National Laboratory and Brookhaven National Laboratory.

Note: This page contains sample records for the topic "dna double helix" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


321

Electromagnetic Signals from Bacterial DNA  

E-Print Network [OSTI]

Chemical reactions can be induced at a distance due to the propagation of electromagnetic signals during intermediate chemical stages. Although is is well known at optical frequencies, e.g. photosynthetic reactions, electromagnetic signals hold true for muck lower frequencies. In E. coli bacteria such electromagnetic signals can be generated by electric transitions between energy levels describing electrons moving around DNA loops. The electromagnetic signals between different bacteria within a community is a "wireless" version of intercellular communication found in bacterial communities connected by "nanowires". The wireless broadcasts can in principle be of both the AM and FM variety due to the magnetic flux periodicity in electron energy spectra in bacterial DNA orbital motions.

A. Widom; J. Swain; Y. N. Srivastava; S. Sivasubramanian

2012-02-09T23:59:59.000Z

322

2005 Double white lines on bills of Razorbill 103 DOUBLE WHITE LINES ON THE BILL OF THE  

E-Print Network [OSTI]

2005 Double white lines on bills of Razorbill 103 DOUBLE WHITE LINES ON THE BILL OF THE RAZORBILL.L., MUZAFFAR S.B. & JONES I.L. Lavers J.L., Muzaffar S.B. & Jones I.L. 2006. Double white lines on the bill Seabirds 7(3): 103-108. One of the diagnostic features of the Razorbill Alca torda is the distinct white

Jones, Ian L.

323

A hybrid double-dot in silicon  

SciTech Connect (OSTI)

We report electrical measurements of a single arsenic dopant atom in the tunnel barrier of a silicon single-electron transistor (SET). In addition to performing the electrical characterisation of the individual dopants, we study the series electrical transport through the dopant and SET. We measure the bias triangles of this hybrid double-dot and show that we can tune the electrostatic coupling between the two sub-systems. Additionally, we measured SET in which an additional plunger gate allows the reduction of the electron number in the SET down to the few-electron regime where the dot presents well-defined spin configurations. Finally, we discuss the challenges of operating a dopant-dot hybrid system in the few-electron regime.

Gonzalez-Zalba, M. F.; Heiss, D.; Ferguson, A. J. [Cavendish Laboratory, University of Cambridge, JJ Thomson Avenue, Cambridge (United Kingdom)

2013-12-04T23:59:59.000Z

324

Efficient jitter compensation using double exponential smoothing  

Science Journals Connector (OSTI)

This paper proposes a new jitter reduction scheme based on double exponential smoothing (DES). We compare this DES-based method to jitter reduction methods based on the Kalman filter (KF) and extended Kalman filter (EKF), two well-known methods of jitter reduction. To evaluate the jitter reduction performance, we used a laser pointer interaction system with a known problem with jittery laser spot movements caused by natural hand tremors. We show that the DES-based scheme runs approximately 100 times faster than the EKF-based method and 19 times faster than the KF-based method. Furthermore, in terms of jitter reduction, the proposed DES-based method yields approximately 18% better results than the EKF-based method and 20% better results than the KF-based method.

Min Gyo Chung; Sang-Kyun Kim

2013-01-01T23:59:59.000Z

325

Range Separated Brueckner Coupled Cluster Doubles Theory  

E-Print Network [OSTI]

We introduce a range-separation approximation to coupled cluster doubles (CCD) theory that successfully overcomes limitations of regular CCD when applied to the uniform electron gas. We combine the short-range ladder channel with the long-range ring channel in the presence of a Bruckner renormalized one-body interaction and obtain ground-state energies with an accuracy of 0.001 a.u./electron across a wide range of density regimes. Our scheme is particularly useful in the low-density and strongly-correlated regimes, where regular CCD has serious drawbacks. Moreover, we cure the infamous overcorrelation of approaches based on ring diagrams (i.e. the particle-hole random phase approximation). Our energies are further shown to have appropriate basis set and thermodynamic limit convergence, and overall this scheme promises energetic properties for realistic periodic and extended systems which existing methods do not possess.

Shepherd, James J; Scuseria, Gustavo E

2013-01-01T23:59:59.000Z

326

Experimental searches of neutrinoless double beta decay  

E-Print Network [OSTI]

Neutrinoless double decay is a unique probe for lepton number conservation and neutrino properties. It allows to investigate the Dirac/Majorana nature of the neutrinos and their absolute mass scale (hierarchy problem) with unprecedented sensitivity. A number of experiments are presently under preparation to cover the quasi-degenerate region of the neutrino mass spectrum. Improved sensitivities are however required to sound the so-called inverted hierarchy region. This is a real challenge faced by a number of new proposed projects, based either on large expansions of the present experiments or on new ideas top improve the technical performance or reduce the background contributions. A review of the most relevant ongoing experiments is given. The most relevant parameters contributing to the experimental sensitivity are discussed and a critical comparison of the future projects is proposed.

Oliviero Cremonesi

2012-12-19T23:59:59.000Z

327

Double Shell Tank (DST) Utilities Specification  

SciTech Connect (OSTI)

This specification establishes the performance requirements and provides the references to the requisite codes and standards to he applied during the design of the Double-Shell Tank (DST) Utilities Subsystems that support the first phase of waste feed delivery (WFD). The DST Utilities Subsystems provide electrical power, raw/potable water, and service/instrument air to the equipment and structures used to transfer low-activity waste (LAW) and high-level waste (HLW) to designated DST staging tanks. The DST Utilities Subsystems also support the equipment and structures used to deliver blended LAW and HLW feed from these staging tanks to the River Protection Project (RPP) Privatization Contractor facility where the waste will be immobilized. This specification is intended to be the basis for new projects/installations. This specification is not intended to retroactively affect previously established project design criteria without specific direction by the program.

SUSIENE, W.T.

2000-04-27T23:59:59.000Z

328

Correlations and the neutrinoless double beta decay  

Science Journals Connector (OSTI)

We explore the influence of the deformation on the nuclear matrix elements of the neutrinoless double beta decay (NME) concluding that the difference in deformation ?or more generally on the amount of quadrupole correlations? between parent and grand daughter nuclei quenchs strongly the decay. We discuss how varies the nuclear matrix element of 76 Ge decay when the wave functions of the two nuclei involved in the transition are constrained to reproduce the experimental occupancies. In the Interacting Shell Model description the value of the NME is enhanced about 15% compared to previous calculations whereas in the QRPA the NME’s are reduced by 20%–30% thus the discrepancies between both approaches diminish.

J. Menéndez; A. Poves; E. Caurier; F. Nowacki

2009-01-01T23:59:59.000Z

329

Deep subwavelength plasmonic waveguide switch in double graphene layer structure  

E-Print Network [OSTI]

Deep subwavelength plasmonic waveguide switch in double graphene layer structure Hideo Iizuka in double graphene layer structure Hideo Iizuka1,a) and Shanhui Fan2,b) 1 Toyota Central Research December 2013) Graphene provides excellent prospects of the dynamic tunability, low propagation loss

Fan, Shanhui

330

Double quantum dot as a minimal thermoelectric generator  

Science Journals Connector (OSTI)

Based on numerical renormalization group calculations, we demonstrate that experimentally realized double quantum dots constitute a minimal thermoelectric generator. In the Kondo regime, one quantum dot acts as an n-type and the other one as a p-type thermoelectric device. Properly connected, a capacitively coupled double quantum dot provides a miniature power supply utilizing the thermal energy of the environment.

S. Donsa; S. Andergassen; K. Held

2014-03-05T23:59:59.000Z

331

Strategies for Next Generation Neutrinoless Double-Beta Decay Experiments  

E-Print Network [OSTI]

Strategies for Next Generation Neutrinoless Double-Beta Decay Experiments F. T. Avignone III A brief discussion of the connection between neutrino oscillation data and predictions of neutrinoless the necessary tools for comparative evaluation. 1. INTRODUCTION Neutrinoless double-beta (0)-decay has been

332

Neutrinoless double-beta decay. A brief review  

E-Print Network [OSTI]

In this brief review we discuss the generation of Majorana neutrino masses through the see-saw mechanism, the theory of neutrinoless double-beta decay, the implications of neutrino oscillation data for the effective Majorana mass, taking into account the recent Daya Bay measurement of theta_13, and the interpretation of the results of neutrinoless double-beta decay experiments.

S. M. Bilenky; C. Giunti

2012-03-23T23:59:59.000Z

333

The fraction of double degenerates among DA white dwarfs  

E-Print Network [OSTI]

We present the results of a radial velocity survey designed to measure the fraction of double degenerates among DA white dwarfs. The narrow core of the H-alpha line was observed twice or more for 46 white dwarfs yielding radial velocities accurate to a few km/s. This makes our survey the most sensitive to the detection of double degenerates undertaken to date. We found no new double degenerates in our sample, though H-alpha emission from distant companions is seen in two systems. Two stars known to be double degenerates prior to our observations are included in the analysis. We find a 95% probability that the fraction of double degenerates among DA white dwarfs lies in the range [0.017,0.19].

P. F. L. Maxted; T. R. Marsh

1999-01-20T23:59:59.000Z

334

Single- and double-charge exchange at low pion energies  

SciTech Connect (OSTI)

A review is given of pion single- and double-charge exchange reactions at incident energies of 25 to 65 MeV leading to isobaric analog states, and in the case of double-charge exchange leading to the ground state of the residual nucleus. The crucial role of the higher nuclear transparency at low pion energies for the analysis of the data in terms of single and double scattering is demonstrated. The large effects on double-charge exchange produced by the spatial correlations in nuclear wave functions are evident. The data on 1f{sub 7/2} nuclei at 35 MeV are used to establish the general validity of a shell-model-based two-amplitude model for these transitions. Recent measurements of the energy dependence between 25 and 65 MeV of double-charge exchange cross sections at forward angles are presented and discussed. 33 refs., 19 figs.

Baer, H.W.

1991-01-01T23:59:59.000Z

335

Single molecule studies of DNA mismatch repair  

Science Journals Connector (OSTI)

Abstract DNA mismatch repair, which involves is a widely conserved set of proteins, is essential to limit genetic drift in all organisms. The same system of proteins plays key roles in many cancer related cellular transactions in humans. Although the basic process has been reconstituted in vitro using purified components, many fundamental aspects of DNA mismatch repair remain hidden due in part to the complexity and transient nature of the interactions between the mismatch repair proteins and DNA substrates. Single molecule methods offer the capability to uncover these transient but complex interactions and allow novel insights into mechanisms that underlie DNA mismatch repair. In this review, we discuss applications of single molecule methodology including electron microscopy, atomic force microscopy, particle tracking, FRET, and optical trapping to studies of DNA mismatch repair. These studies have led to formulation of mechanistic models of how proteins identify single base mismatches in the vast background of matched DNA and signal for their repair.

Dorothy A. Erie; Keith R. Weninger

2014-01-01T23:59:59.000Z

336

Enhancing the DNA Patent Database  

SciTech Connect (OSTI)

Final Report on Award No. DE-FG0201ER63171 Principal Investigator: LeRoy B. Walters February 18, 2008 This project successfully completed its goal of surveying and reporting on the DNA patenting and licensing policies at 30 major U.S. academic institutions. The report of survey results was published in the January 2006 issue of Nature Biotechnology under the title “The Licensing of DNA Patents by US Academic Institutions: An Empirical Survey.” Lori Pressman was the lead author on this feature article. A PDF reprint of the article will be submitted to our Program Officer under separate cover. The project team has continued to update the DNA Patent Database on a weekly basis since the conclusion of the project. The database can be accessed at dnapatents.georgetown.edu. This database provides a valuable research tool for academic researchers, policymakers, and citizens. A report entitled Reaping the Benefits of Genomic and Proteomic Research: Intellectual Property Rights, Innovation, and Public Health was published in 2006 by the Committee on Intellectual Property Rights in Genomic and Protein Research and Innovation, Board on Science, Technology, and Economic Policy at the National Academies. The report was edited by Stephen A. Merrill and Anne-Marie Mazza. This report employed and then adapted the methodology developed by our research project and quoted our findings at several points. (The full report can be viewed online at the following URL: http://www.nap.edu/openbook.php?record_id=11487&page=R1). My colleagues and I are grateful for the research support of the ELSI program at the U.S. Department of Energy.

Walters, LeRoy B.

2008-02-18T23:59:59.000Z

337

Self-Assembly of Metal-DNA Triangles and DNA Nanotubes with Synthetic Junctions  

Science Journals Connector (OSTI)

While DNA represents one of nature’s most predictable ... catalytic properties that are not observed naturally for DNA. Conceptually, this brings the toolbox of ... a diverse array of structures and functions usi...

Hua Yang; Pik Kwan Lo; Christopher K. McLaughlin; Graham D. Hamblin…

2011-01-01T23:59:59.000Z

338

A Synthetic DNA Walker for Molecular Transport  

Science Journals Connector (OSTI)

A Synthetic DNA Walker for Molecular Transport ... The authors report the incrementally staged design, synthesis, characterization, and operation of a mol. ...

Jong-Shik Shin; Niles A. Pierce

2004-08-17T23:59:59.000Z

339

IT for synthetic biology and DNA nanotechnology  

Science Journals Connector (OSTI)

Somewhere between the studies of information technology and organic chemistry, researchers are trying to make tiny robots out of DNA molecules.

Masami Hagiya; Fumiaki Tanaka; Ibuki Kawamata

2010-09-01T23:59:59.000Z

340

j5 DNA Assembly Design Automation Software  

Science Journals Connector (OSTI)

j5 DNA Assembly Design Automation Software ... Here, we report the development and deployment of a web-based software tool, j5, which automates the design of scar-less multipart DNA assembly protocols including SLIC, Gibson, CPEC, and Golden Gate. ... The key innovations of the j5 design process include cost optimization, leveraging DNA synthesis when cost-effective to do so, the enforcement of design specification rules, hierarchical assembly strategies to mitigate likely assembly errors, and the instruction of manual or automated construction of scar-less combinatorial DNA libraries. ...

Nathan J. Hillson; Rafael D. Rosengarten; Jay D. Keasling

2011-12-07T23:59:59.000Z

Note: This page contains sample records for the topic "dna double helix" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


341

First results on neutrinoless double beta decay of Te-130 with the calorimetric cuoricino experiment  

E-Print Network [OSTI]

Evidence for Neutrinoless Double Beta Decay” arXiv:hep-on “Evidence for neutrinoless double beta decay”- arXiv:hep-Results on Neutrinoless Double Beta Decay of 130 Te with the

2003-01-01T23:59:59.000Z

342

Codeword design and information encoding in DNA ensembles  

Science Journals Connector (OSTI)

Encoding of information in DNA-, RNA- and other biomolecules is an important area of research in fields such as DNA computing, bioinformatics, and, conceivably, microbiology and genetics. This survey focuses on two fundamental problems, the codeword ... Keywords: h-distance, DNA space dimension, DNA-based computing, Gibbs energy, codeword design, information encoding in DNA, partition function, template method, tensor product

Max H. Garzon; Russell J. Deaton

2004-08-01T23:59:59.000Z

343

Creating Directed Double-strand Breaks with the Ref Protein: A Novel Rec A-Dependent Nuclease from Bacteriophage P1  

SciTech Connect (OSTI)

The bacteriophage P1-encoded Ref protein enhances RecA-dependent recombination in vivo by an unknown mechanism. We demonstrate that Ref is a new type of enzyme; that is, a RecA-dependent nuclease. Ref binds to ss- and dsDNA but does not cleave any DNA substrate until RecA protein and ATP are added to form RecA nucleoprotein filaments. Ref cleaves only where RecA protein is bound. RecA functions as a co-nuclease in the Ref/RecA system. Ref nuclease activity can be limited to the targeted strands of short RecA-containing D-loops. The result is a uniquely programmable endonuclease activity, producing targeted double-strand breaks at any chosen DNA sequence in an oligonucleotide-directed fashion. We present evidence indicating that cleavage occurs in the RecA filament groove. The structure of the Ref protein has been determined to 1.4 {angstrom} resolution. The core structure, consisting of residues 77-186, consists of a central 2-stranded {beta}-hairpin that is sandwiched between several {alpha}-helical and extended loop elements. The N-terminal 76 amino acid residues are disordered; this flexible region is required for optimal activity. The overall structure of Ref, including several putative active site histidine residues, defines a new subclass of HNH-family nucleases. We propose that enhancement of recombination by Ref reflects the introduction of directed, recombinogenic double-strand breaks.

Gruenig, Marielle C.; Lu, Duo; Won, Sang Joon; Dulberger, Charles L.; Manlick, Angela J.; Keck, James L.; Cox, Michael M. (UW)

2012-03-16T23:59:59.000Z

344

Half-lives of Double $\\beta ^+$-decay with Two Neutrinos  

E-Print Network [OSTI]

Nuclear double $\\beta ^-$-decays with two neutrinos were observed for many years and a systematic law describing the relation between their half-lives and decay energies was also proposed recently [Phys. Rev. C89, 064603 (2014)]. However, double $\\beta ^+$-decay ($\\beta ^+\\beta^+)$ with emission of both two positrons and two neutrinos has not been observed up to date. In this article, we perform a systematic analysis on the candidates of double $\\beta ^+$-decay, based on the 2012 nuclear mass table. Eight nuclei are found to be the good candidates for double $\\beta ^+$-decay and their half-lives are predicted according to the generalization of the systematic law to double $\\beta ^+$-decay. As far as we know, there is no theoretical result on double $\\beta ^+$-decay of nucleus $^{154}Dy$ and our result is the first prediction on this nucleus. This is also the first complete research on eight double $\\beta ^+$-decay candidates based on the available data of nuclear masses. It is expected that the calculated hal...

Ren, Yuejiao

2015-01-01T23:59:59.000Z

345

Comparison of genomic, plasmid, synthetic, and combined DNA probes for detecting Plasmodium falciparum DNA.  

Science Journals Connector (OSTI)

...pRepHind, and a 21-base-long synthetic DNA probe (PFR1), the sequence...second brief hybridization with synthetic DNA to amplify signals from samples...pRepHind, and a 21-base-long synthetic DNA probe (PFR1), the sequence...

G L McLaughlin; W E Collins; G H Campbell

1987-05-01T23:59:59.000Z

346

DNA barcoding of stranded marine mammals 5 The use of DNA barcoding to monitor the marine  

E-Print Network [OSTI]

barcoding to monitor the marine mammal biodiversity along the French Atlantic coast. In: Nagy ZT, Backeljau of the monitoring of marine mammal biodiversity. Keywords DNA barcoding, COI, control region, marine mammalsDNA barcoding of stranded marine mammals 5 The use of DNA barcoding to monitor the marine mammal

Paris-Sud XI, UniversitƩ de

347

Experimental research of double beta decay of atomic nuclei  

E-Print Network [OSTI]

Results of several double beta decay experiments, performed with the help of low background crystal scintillators, are presented. In particular, the half-life value of the two-neutrino double beta decay of 116-Cd has been measured as 2.9 10^{19} yr, and the new half-life limit on the neutrinoless double beta decay of 116-Cd has been established as >1.7 10^{23} yr at 90%, which corresponds to a restriction on the neutrino mass <1.7 eV. New half-life bounds on the level of 10^{17}-10^{21} yr were set for double beta processes in 64-Zn, 70-Zn, 106-Cd, 108-Cd, 114-Cd, 136-Ce, 138-Ce, 142-Ce, 160-Gd, 180-W, and 186-W by using low-background CdWO4, GSO, and ZnWO4 crystal scintillators. The claim of discovery of the neutrinoless double beta decay of 76-Ge [Mod. Phys. Lett. A 16 (2001) 2409] was analyzed. The demands of the future high sensitivity double beta decay experiments, aiming to observe the neutrinoless double beta decay or to advance restrictions on the neutrino mass to < 0.01 eV, were considered. Requirements for their sensitivity and discovery potential were formulated. Two projects of double beta experiments with a sensitivity on the level of 10^{26}-10^{27} yr (CAMEO and CARVEL projects) were discussed. Scintillation properties and radioactive contamination of CaWO4, ZnWO4, CdWO4, PbWO4, GSO(Ce), CeF3, yttrium-aluminum garnet doped with neodymium (YAG:Nd) crystal scintillators were studied. Applicability of these scintillators to search for double beta decay was discussed.

F. A. Danevich

2011-12-26T23:59:59.000Z

348

HolT Hunter: Software for Identifying and Characterizing Low-Strain DNA Holliday Triangles  

SciTech Connect (OSTI)

Synthetic DNA nanostructures are most commonly held together via Holliday junctions. These junctions allow for a wide variety of different angles between the double helices they connect. Nevertheless, only constructs with a very limited selection of angles have been built, to date, because of the computational complexity of identifying structures that fit together with low strain at odd angles. I have developed an algorithm that finds over 95% of the possible solutions by breaking the problem down into two portions. First, there is a problem of how smooth rods can form triangles by lying across one another. This problem is easily handled by numerical computation. Second, there is the question of how distorted DNA double helices would need to be to fit onto the rod structure. This strain is calculated directly. The algorithm has been implemented in a Mathematica 8 notebook called Holliday Triangle Hunter. A large database of solutions has been identified. Additional interface software is available to facilitate drawing and viewing models.

Sherman W. B.

2012-06-05T23:59:59.000Z

349

Superconvergence of period doubling cascade in trapezoid maps  

E-Print Network [OSTI]

In the symmetric and the asymmetric trapezoid maps, as a slope of the trapezoid is increased, the period doubling cascade occurs and the symbolic sequence of periodic points is the Metropolis-Stein-Stein sequence and the convergence of the onset point of the period 2^m solution to the accumulation point is exponentially fast. We reported these results previously. In this paper, we give the detailed description of the proof on the results. Further, we study the period doubling cascade starting from period p solution and show the superconvergence of the period doubling cascade.

T. Uezu

2000-01-12T23:59:59.000Z

350

Photo-double-ionization of the nitrogen molecule  

Science Journals Connector (OSTI)

The triple differential cross sections of the photo-double-ionization of the nitrogen molecule to the X 1?+g and a3?u N22+ states have been measured at about 20 eV above their respective ionization thresholds in the equal energy sharing kinematics and calculated using a model which makes use of correlated two-center double continuum wave functions. The comparison of the results with those obtained by the Gaussian parametrization method applied in the past with success to heliumlike targets shows the influence of the molecular nature of the N2 target in the photo-double-ionization.

P. Bolognesi; B. Joulakian; A. A. Bulychev; O. Chuluunbaatar; L. Avaldi

2014-05-07T23:59:59.000Z

351

Short fuzzy tandem repeats in genomic sequences, identification, and possible role in regulation of gene expression  

Science Journals Connector (OSTI)

......experimental data suggest that certain 3-periodic synthetic DNA sequences have substantially different helix stability...experimental data suggest that certain 3-periodic synthetic DNA sequences have substantially different helix stability......

Valentina Boeva; Mireille Regnier; Dmitri Papatsenko; Vsevolod Makeev

2006-03-15T23:59:59.000Z

352

Magnetic study of the electronic states in B-DNA and M-DNA doped with metal ions Kenji Mizoguchi,  

E-Print Network [OSTI]

- tivity of synthetic DNA (poly(G)-poly(C)) with a length of 10 nm to find a semiconducting behaviorMagnetic study of the electronic states in B-DNA and M-DNA doped with metal ions Kenji Mizoguchi. Purified salmon DNA gives intrinsically no EPR signal, which is consistent with DNA being a semiconductor

Mizoguchi, Kenji

353

E-Print Network 3.0 - aligned double-walled carbon Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Summary: , and H. M. Cheng. Polarized raman analysis of aligned double- walled carbon nanotubes. Physical Review B... Nonlinear Oscillations of a Double-Walled Carbon Nanotube...

354

Neutrinoless double beta decay in deformed nuclei: its implications in particle and nuclear physics .  

E-Print Network [OSTI]

??In my thesis, we calculated the Nuclear Matrix Elements (NME) for neutrinoless double beta decay (0??? decay). Neutrinoless double beta decay is a rare nuclear… (more)

Fang, DongLiang

2011-01-01T23:59:59.000Z

355

SciTech Connect: What can we learn from neutrinoless double beta...  

Office of Scientific and Technical Information (OSTI)

What can we learn from neutrinoless double beta decay experiments? Citation Details In-Document Search Title: What can we learn from neutrinoless double beta decay experiments?...

356

A Search for Neutrinoless Double-Beta Decay with EXO-200 .  

E-Print Network [OSTI]

??This work presents a search for neutrinoless double beta decay of 136Xe using data from the EXO-200 detector collected between 2011 and 2012. Neutrinoless double… (more)

Slutsky, Simon

2013-01-01T23:59:59.000Z

357

WHERE MULTIFUNCTIONAL DNA REPAIR PROTEINS MEET: MAPPING THE INTERACTION DOMAINS BETWEEN XPG AND WRN  

SciTech Connect (OSTI)

The rapid recognition and repair of DNA damage is essential for the maintenance of genomic integrity and cellular survival. Multiple complex and interconnected DNA damage responses exist within cells to preserve the human genome, and these repair pathways are carried out by a specifi c interplay of protein-protein interactions. Thus a failure in the coordination of these processes, perhaps brought about by a breakdown in any one multifunctional repair protein, can lead to genomic instability, developmental and immunological abnormalities, cancer and premature aging. This study demonstrates a novel interaction between two such repair proteins, Xeroderma pigmentosum group G protein (XPG) and Werner syndrome helicase (WRN), that are both highly pleiotropic and associated with inherited genetic disorders when mutated. XPG is a structure-specifi c endonuclease required for the repair of UV-damaged DNA by nucleotide excision repair (NER), and mutations in XPG result in the diseases Xeroderma pigmentosum (XP) and Cockayne syndrome (CS). A loss of XPG incision activity results in XP, whereas a loss of non-enzymatic function(s) of XPG causes CS. WRN is a multifunctional protein involved in double-strand break repair (DSBR), and consists of 3’–5’ DNA-dependent helicase, 3’–5’ exonuclease, and single-strand DNA annealing activities. Nonfunctional WRN protein leads to Werner syndrome, a premature aging disorder with increased cancer incidence. Far Western analysis was used to map the interacting domains between XPG and WRN by denaturing gel electrophoresis, which separated purifi ed full length and recombinant XPG and WRN deletion constructs, based primarily upon the length of each polypeptide. Specifi c interacting domains were visualized when probed with the secondary protein of interest which was then detected by traditional Western analysis using the antibody of the secondary protein. The interaction between XPG and WRN was mapped to the C-terminal region of XPG as well as the C-terminal region of WRN. The physical interaction between XPG and WRN links NER, (made evident by the disease XP) with DSBR, which imparts additional knowledge of the overlapping nature of these two proteins and the previously distinct DNA repair pathways they are associated with. Since genomic integrity is constantly threatened by both endogenous and exogenous (internal and external) damage, understanding the roles of these proteins in coordinating DNA repair processes with replication will signifi cantly further understanding how defects instigate physiological consequences in response to various DNA damaging sources. This ultimately contributes to our understanding of cancer and premature aging.

Rangaraj, K.; Cooper, P.K.; Trego, K.S.

2009-01-01T23:59:59.000Z

358

Property:NEPA DNA Worksheet | Open Energy Information  

Open Energy Info (EERE)

DNA Worksheet DNA Worksheet Jump to: navigation, search Property Name NEPA DNA Worksheet Property Type Page Description DNA Worksheet files for NEPA Docs. This is a property of type Page. It links to pages that use the form NEPA_Doc. Pages using the property "NEPA DNA Worksheet" Showing 19 pages using this property. D DOI-BLM-NV-C010-2011-0517-DNA + DOI-BLM-NV-C010-2011-0517-DNA.pdf + DOI-BLM-NV-C010-2012--044-DNA + DOI-BLM-NV-C010-2012-0044-DNA.pdf + DOI-BLM-NV-C010-2012-0005-DNA + DOI-BLM-NV-C010-2012-0005-DNA.pdf + DOI-BLM-NV-C010-2012-0016-DNA + DOI-BLM-NV-C010-2012-0016-DNA.pdf + DOI-BLM-NV-C010-2012-0019-DNA + DOI-BLM-NV-C010-2012-0019-DNA.pdf + DOI-BLM-NV-C010-2012-0020-DNA + DOI-BLM-NV-C010-2012-0020-DNA.pdf + DOI-BLM-NV-C010-2012-0028-DNA + DOI-BLM-NV-C010-2012-0028-DNA.pdf +

359

Mass Transfer between Double White Dwarfs  

E-Print Network [OSTI]

Three periodically variable stars have recently been discovered (V407 Vul, P=9.5 min; ES Cet, P=10.3 min; RX J0806.3+1527, P=5.3 min) with properties that suggest that their photometric periods are also their orbital periods, making them the most compact binary stars known. If true, this might indicate that close, detached, double white dwarfs are able to survive the onset of mass transfer caused by gravitational wave radiation and emerge as the semi-detached, hydrogen-deficient stars known as the AM CVn stars. The accreting white dwarfs in such systems are large compared to the orbital separations. This has two effects: first it makes it likely that the mass transfer stream can hit the accretor directly, and second it causes a loss of angular momentum from the orbit which can destabilise the mass transfer unless the angular momentum lost to the accretor can be transferred back to the orbit. The effect of the destabilisation is to reduce the number of systems which survive mass transfer by as much as one hundred-fold. In this paper we analyse this destabilisation and the stabilising effect of a dissipative torque between the accretor and the binary orbit. We obtain analytic criteria for the stability of both disc-fed and direct impact accretion, and carry out numerical integrations to assess the importance of secondary effects, the chief one being that otherwise stable systems can exceed the Eddington accretion rate. We show that to have any effect upon survival rates, the synchronising torque must act on a timescale of order 1000 years or less. If synchronisation torques are this strong, then they will play a significant role in the spin rates of white dwarfs in cataclysmic variable stars as well.

T. R. Marsh; G. Nelemans; D. Steeghs

2003-12-22T23:59:59.000Z

360

Carbon Nanotube DNA Sensor and Sensing Mechanism  

E-Print Network [OSTI]

Carbon Nanotube DNA Sensor and Sensing Mechanism Xiaowu Tang,*,, Sarunya Bansaruntip, Nozomi; Revised Manuscript Received June 7, 2006 ABSTRACT We report the fabrication of single-walled carbon nanotube (SWNT) DNA sensors and the sensing mechanism. The simple and generic protocol for label

Le Roy, Robert J.

Note: This page contains sample records for the topic "dna double helix" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


361

Mass Spectrometry and Density Functional Theory Characterizations of DNA Modifications  

E-Print Network [OSTI]

to fully characterize synthetic DNA bearing cisplatin cross-in the hydrolysis of synthetic DNA as part of the MS-basedlink yield from synthetic DNA harboring 5-X-pyrimidines,

Williams, Renee Therese

2012-01-01T23:59:59.000Z

362

Stabilizing synthetic data in the DNA of living organisms  

Science Journals Connector (OSTI)

Data-encoding synthetic DNA, inserted into the genome of a living ... generations, one of the merits of using DNA material is long-term data storage within ... broken by mutation, deletion, and insertion of DNA, ...

Nozomu Yachie; Yoshiaki Ohashi; Masaru Tomita

2008-06-01T23:59:59.000Z

363

Single-molecule studies of DNA dynamics and intermolecular forces  

E-Print Network [OSTI]

in relating DNA solutions to synthetic polymer melts. In any?x or ?y. DNA and numerous synthetic polymer solutions havethe other hand, DNA differs from many synthetic polymers in

Robertson, Rae Marie

2007-01-01T23:59:59.000Z

364

Mechanism of Interaction of CC-1065 (NSC 298223) with DNA  

Science Journals Connector (OSTI)

...interaction of CC-1065 with synthetic DNA polymers indicated a specificity...interaction of CC-1065 with synthetic DNA polymers indicated a specificity...interaction of CC-1065 with synthetic DNA polymers indicated a specificity...

David H. Swenson; Li H. Li; Laurence H. Hurley; J. Stefan Rokem; Gary L. Petzold; Brian D. Dayton; Tanya L. Wallace; Alice H. Lin; William C. Krueger

1982-07-01T23:59:59.000Z

365

Mass Spectrometry and Density Functional Theory Characterizations of DNA Modifications  

E-Print Network [OSTI]

link yield from synthetic DNA harboring 5-X-pyrimidines,dU- and Br dC-containing synthetic DNA were reported, it wasthe phosphoramidites into synthetic DNA at a 5’-CpG-3’ site;

Williams, Renee Therese

2012-01-01T23:59:59.000Z

366

Electric DNA chips for determination of pathogenic microorganisms  

E-Print Network [OSTI]

Electric DNA chips for determination of pathogenic microorganisms Yanling Liu Doctoral thesis Liu (2008): Electric DNA chips for determination of pathogenic microorganisms. School of Biotechnology, Royal Institute of Technology (KTH), Stockholm, Sweden Abstract Silicon-based electric DNA chip arrays

Enfors, Sven-Olof

367

Single molecule analysis of DNA electrophoresis in microdevices  

E-Print Network [OSTI]

Given that current electrophoresis technology is inadequate for mapping large O[100 kilobasepair] DNA, several promising lab-on-chip designs for DNA mapping have been recently proposed that require either 1) a DNA molecule ...

Randall, Greg C

2006-01-01T23:59:59.000Z

368

Mitochondrial DNA phylogenetic analysis of Myrmecolacidae (Insecta: Strepsiptera) va  

E-Print Network [OSTI]

Guinea are determined. Methods were developed for extraction, amplification, and sequencing of mitochondrial DNA (mtDNA) from Strepsiptera. High-resolution mtDNA sequences obtained by these methods were used in the phylogenetic analysis of Myrmecolacidae...

Halbert, Natalie Rose

2013-02-22T23:59:59.000Z

369

Allosteric Modulation of DNA by Small Molecules  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Allosteric Modulation of DNA by Small Allosteric Modulation of DNA by Small Molecules Signals originating at the cell surface are conveyed by a complex system of interconnected signaling pathways to the nucleus. They converge at transcription factors, which in turn regulate the transcription of sets of genes that result in the gene expression. Many human diseases are caused by dysregulated gene expression and the oversupply of transcription factors may be required for the growth and metastatic behavior of human cancers. Cell permeable small molecules that can be programmed to disrupt transcription factor-DNA interfaces could silence aberrant gene expression pathways. Pyrrole-imidazole polyamides are DNA minor groove binding small molecules that are programmable for a large repertoire of DNA motifs.

370

DNA nanotechnology: understanding and optimisation through simulation  

E-Print Network [OSTI]

DNA nanotechnology promises to provide controllable self-assembly on the nanoscale, allowing for the design of static structures, dynamic machines and computational architectures. In this article I review the state-of-the art of DNA nanotechnology, highlighting the need for a more detailed understanding of the key processes, both in terms of theoretical modelling and experimental characterisation. I then consider coarse-grained models of DNA, mesoscale descriptions that have the potential to provide great insight into the operation of DNA nanotechnology if they are well designed. In particular, I discuss a number of nanotechnological systems that have been studied with oxDNA, a recently developed coarse-grained model, highlighting the subtle interplay of kinetic, thermodynamic and mechanical factors that can determine behaviour. Finally, new results highlighting the importance of mechanical tension in the operation of a two-footed walker are presented, demonstrating that recovery from an unintended `overstepp...

Ouldridge, Thomas E

2014-01-01T23:59:59.000Z

371

DUF6 Project Doubles Production in 2013 | Department of Energy  

Broader source: Energy.gov (indexed) [DOE]

DUF6 Project Doubles Production in 2013 DUF6 Project Doubles Production in 2013 DUF6 Project Doubles Production in 2013 November 26, 2013 - 12:00pm Addthis LEXINGTON, Ky. - The conversion plants at EM's Paducah and Portsmouth sites surpassed a fiscal year 2013 goal by converting 13,679 metric tons of depleted uranium hexafluoride (DUF6), more than doubling production a year earlier. EM's Portsmouth Paducah Project Office (PPPO) and contractor Babcock & Wilcox Conversion Services LLC (BWCS) began operations in 2011 to convert the nation's 800,000-metric-ton inventory of DUF6 to more benign forms for sale, ultimate disposal or long-term storage. "Since 2011, we have been ramping up production to determine and achieve the safe, sustainable operating rate of the plants," said George E.

372

Non-Double-Couple Microearthquakes At Long Valley Caldera, California,  

Open Energy Info (EERE)

Non-Double-Couple Microearthquakes At Long Valley Caldera, California, Non-Double-Couple Microearthquakes At Long Valley Caldera, California, Provide Evidence For Hydraulic Fracturing Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Journal Article: Non-Double-Couple Microearthquakes At Long Valley Caldera, California, Provide Evidence For Hydraulic Fracturing Details Activities (1) Areas (1) Regions (0) Abstract: Most of 26 small (0.4<~M<~3.1) microearthquakes at Long Valley caldera in mid-1997, analyzed using data from a dense temporary network of 69 digital three-component seismometers, have significantly non-double-couple focal mechanisms, inconsistent with simple shear faulting. We determined their mechanisms by inverting P- and S-wave polarities and amplitude ratios using linear-programming methods, and

373

DUF6 Project Doubles Production in 2013 | Department of Energy  

Broader source: Energy.gov (indexed) [DOE]

DUF6 Project Doubles Production in 2013 DUF6 Project Doubles Production in 2013 DUF6 Project Doubles Production in 2013 November 26, 2013 - 12:00pm Addthis LEXINGTON, Ky. - The conversion plants at EM's Paducah and Portsmouth sites surpassed a fiscal year 2013 goal by converting 13,679 metric tons of depleted uranium hexafluoride (DUF6), more than doubling production a year earlier. EM's Portsmouth Paducah Project Office (PPPO) and contractor Babcock & Wilcox Conversion Services LLC (BWCS) began operations in 2011 to convert the nation's 800,000-metric-ton inventory of DUF6 to more benign forms for sale, ultimate disposal or long-term storage. "Since 2011, we have been ramping up production to determine and achieve the safe, sustainable operating rate of the plants," said George E.

374

Kinetic Alfven double layer formed by electron viscosity  

SciTech Connect (OSTI)

The effect of the electron viscosity on the kinetic Alfven solitary wave is investigated. It is found that small electron viscosity changes the electron motion along the magnetic field producing a boundary layer, and thus that in a low beta electron-ion plasma({beta} Much-Less-Than m{sub e}/m{sub i}), an obliquely propagating kinetic solitary Alfven wave can become a double layer. This double layer can exist in the sub-Alfvenic and super-Alfvenic regimes. The length scale of density drop for this double layer is on the order of that of the conventional kinetic solitary Alfven wave, and thus this double layer can accelerate electrons on a very short length scale.

Woo, M. H.; Ryu, C.-M. [Department of Physics, Pohang University of Science and Technology, Pohang 790-784 (Korea, Republic of); Choi, C. R. [Department of Physics, Korea Advanced Institute of Science and Technology, Taejon 305-701 (Korea, Republic of)

2012-07-15T23:59:59.000Z

375

Superconductivity in Bundles of Double-Wall Carbon Nanotubes  

E-Print Network [OSTI]

We present electrical and thermal specific heat measurements that show superconductivity in double-wall carbon nanotube (DWCNT) bundles. Clear evidence, comprising a resistance drop as a function of temperature, magnetoresistance ...

Shi, Wu

376

Characterization of double walled carbon nanotubes-polyvinylidene fluoride nanocomposites  

E-Print Network [OSTI]

One of the main objectives of this thesis is to disperse double-walled carbon nanotubes (DWNT) in a polyvinylidene fluoride (PVDF) matrix, and to characterize the resulting composite using electrical, thermal, and mechanical characterization...

Almasri, Atheer Mohammad

2007-04-25T23:59:59.000Z

377

Student's difficulties with the theory of double-slit interference  

E-Print Network [OSTI]

developed abstract thinking abilities [3, 4]. The remainder are unable to learn physics by listening arrangement for theoretical treatment of double-slit interference. Figure 2. The mechanical device

PlaninĀ?iĆØ, Gorazd

378

Young’s double-slit interference experiment with electrons  

Science Journals Connector (OSTI)

In this short Note we report a method for producing samples containing two nano-sized slits suitable for demonstrating to undergraduate and graduate students the double-slit electron interference experiment in a conventional transmission electron microscope.

S. Frabboni; G. C. Gazzadi; G. Pozzi

2007-01-01T23:59:59.000Z

379

Comment on "Evidence for Neutrinoless Double Beta Decay"  

E-Print Network [OSTI]

We comment on the recent claim for the experimental observation of neutrinoless double-beta decay. We discuss several limitations in the analysis provided in that paper and conclude that there is no basis for the presented claim.

C. E. Aalseth; F. T. Avignone III; A. Barabash; F. Boehm; R. L. Brodzinski; J. I. Collar; P. J. Doe; H. Ejiri; S. R. Elliott; E. Fiorini; R. J. Gaitskell; G. Gratta; R. Hazama; K. Kazkaz; G. S. King III; R. T. Kouzes; H. S. Miley; M. K. Moe; A. Morales; J. Morales; A. Piepke; R. G. H. Robertson; W. Tornow; P. Vogel; R. A. Warner; J. F. Wilkerson

2002-05-22T23:59:59.000Z

380

Double Auction experiments and their relevance for emissions trading  

Science Journals Connector (OSTI)

In this article, we discuss selected methodological problems from previous Double Auction (DA) experiments and analyze the two following questions experimentally. Firstly, does the framing of the decision situati...

Bodo Sturm

2008-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "dna double helix" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


381

Glass Does a Double-Take | Advanced Photon Source  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

| 2004 | 2003 | 2002 2001 | 2000 | 1998 | Subscribe to APS Science Highlights rss feed Glass Does a Double-Take APRIL 4, 2008 Bookmark and Share Predicted phase diagram as a...

382

Double beta decay experiments: beginning of a new era  

E-Print Network [OSTI]

The review of current experiments on search and studying of double beta decay processes is done. Results of the most sensitive experiments are discussed and values of modern limits on effective Majorana neutrino mass ($) are given. New results on two neutrino double beta decay are presented. The special attention is given to new current experiments with mass of studied isotopes more than 100 kg, EXO--200 and KamLAND--Zen. These experiments open a new era in research of double beta decay. In the second part of the review prospects of search for neutrinoless double beta decay in new experiments with sensitivity to $$ at the level of $\\sim 0.01-0.1$ eV are discussed. Parameters and characteristics of the most perspective projects (CUORE, GERDA, MAJORANA, SuperNEMO, EXO, KamLAND--Zen, SNO+) are given.

A. S. Barabash

2012-09-19T23:59:59.000Z

383

Crystal Structure of the Chromodomain Helicase DNA-binding Protein 1 (Chd1) DNA-binding Domain in Complex with DNA  

SciTech Connect (OSTI)

Chromatin remodelers are ATP-dependent machines that dynamically alter the chromatin packaging of eukaryotic genomes by assembling, sliding, and displacing nucleosomes. The Chd1 chromatin remodeler possesses a C-terminal DNA-binding domain that is required for efficient nucleosome sliding and believed to be essential for sensing the length of DNA flanking the nucleosome core. The structure of the Chd1 DNA-binding domain was recently shown to consist of a SANT and SLIDE domain, analogous to the DNA-binding domain of the ISWI family, yet the details of how Chd1 recognized DNA were not known. Here we present the crystal structure of the Saccharomyces cerevisiae Chd1 DNA-binding domain in complex with a DNA duplex. The bound DNA duplex is straight, consistent with the preference exhibited by the Chd1 DNA-binding domain for extranucleosomal DNA. Comparison of this structure with the recently solved ISW1a DNA-binding domain bound to DNA reveals that DNA lays across each protein at a distinct angle, yet contacts similar surfaces on the SANT and SLIDE domains. In contrast to the minor groove binding seen for Isw1 and predicted for Chd1, the SLIDE domain of the Chd1 DNA-binding domain contacts the DNA major groove. The majority of direct contacts with the phosphate backbone occur only on one DNA strand, suggesting that Chd1 may not strongly discriminate between major and minor grooves.

Sharma A.; Heroux A.; Jenkins K. R.; Bowman G. D.

2011-12-09T23:59:59.000Z

384

Muon-Induced Backgrounds in Double Chooz Emily Conover  

E-Print Network [OSTI]

to scintillator 8 MeV n capture energy, 30Āµs backgrounds due to muons remain EĀÆe = Ee+ + 10-40keV En + 1.8MeMuon-Induced Backgrounds in Double Chooz Emily Conover University of Chicago Second Thesis and Stopped Muons 9Li and 8He 3. Double Chooz Results 4. Conclusions Timeline/Future work Emily Conover (Univ

385

Band Tunneling through Double Barrier in Bilayer Graphene  

E-Print Network [OSTI]

By taking into account the full four band energy spectrum, we calculate the transmission probability and conductance of electrons across symmetric and asymmetric double potential barrier with a confined interlayer potential difference in bilayer graphene. For energies less than the interlayer coupling \\gamma_{1}, E \\gamma_{1}, we obtain four possible ways for transmission resulting from the two propagating modes. We compute the associated transmission probabilities as well as their contribution to the conductance, study the effect of the double barrier geometry.

Hasan A. Alshehab; Hocine Bahlouli; Abderrahim El Mouhafid; Ahmed Jellal

2014-01-20T23:59:59.000Z

386

Dynamics of microcavity exciton polaritons in a Josephson double dimer  

Science Journals Connector (OSTI)

We study the dynamics of exciton polaritons in a double-well configuration. The system consists of two weakly coupled Bose-Josephson junctions, each corresponding to a different circular polarization of the polaritons, forming a Josephson double dimer. We show that the Josephson oscillation between the wells is strongly coupled to the polarization rotation and that, consequently, Josephson excitation is periodically exchanged between the two polarizations. Linearized analysis agrees well with numerical simulations using typical experimental parameters.

Christine Khripkov; Carlo Piermarocchi; Amichay Vardi

2013-12-10T23:59:59.000Z

387

What can we learn from neutrinoless double beta decay experiments?  

E-Print Network [OSTI]

We assess how well next generation neutrinoless double beta decay and normal neutrino beta decay experiments can answer four fundamental questions. 1) If neutrinoless double beta decay searches do not detect a signal, and if the spectrum is known to be inverted hierarchy, can we conclude that neutrinos are Dirac particles? 2) If neutrinoless double beta decay searches are negative and a next generation ordinary beta decay experiment detects the neutrino mass scale, can we conclude that neutrinos are Dirac particles? 3) If neutrinoless double beta decay is observed with a large neutrino mass element, what is the total mass in neutrinos? 4) If neutrinoless double beta decay is observed but next generation beta decay searches for a neutrino mass only set a mass upper limit, can we establish whether the mass hierarchy is normal or inverted? We base our answers on the expected performance of next generation neutrinoless double beta decay experiments and on simulations of the accuracy of calculations of nuclear matrix elements.

John N. Bahcall; Hitoshi Murayama; Carlos Pena-Garay

2004-03-15T23:59:59.000Z

388

Constraint of DNA on Functionalized Graphene Improves Its Biostability...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Constraint of DNA on Functionalized Graphene Improves Its Biostability and Specificity. Constraint of DNA on Functionalized Graphene Improves Its Biostability and Specificity....

389

Minor Groove Deformability of DNA: A Molecular Dynamics Free...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Minor Groove Deformability of DNA: A Molecular Dynamics Free EnergySimulation Study. Minor Groove Deformability of DNA: A Molecular Dynamics Free EnergySimulation Study. Abstract:...

390

Electrochemical Branched-DNA Assay for Polymerase Chain Reaction...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Branched-DNA Assay for Polymerase Chain Reaction-Free Detection and Quantification of Oncogenes in Messenger RNA Electrochemical Branched-DNA Assay for Polymerase Chain...

391

Isolation of Discrete Nanoparticle-DNA Conjugates for Plasmonic Applications  

E-Print Network [OSTI]

Isolation of Discrete Nanoparticle-DNA Conjugates forABSTRACT. Discrete DNA-gold nanoparticle conjugates with DNABG_Keywords”). GOLD, NANOPARTICLE, NANOCRYSTAL, PLASMON,

Claridge, Shelley

2008-01-01T23:59:59.000Z

392

Unidirectional Scaffold-Strand Arrangement in DNA Origami  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Unidirectional Scaffold-Strand Arrangement in DNA Origami Authors: Han, D., Jiang, S., Samanta, A., Liu, Y., and Yan, H. Title: Unidirectional Scaffold-Strand Arrangement in DNA...

393

Single molecule studies of DNA packaging by bacteriophages  

E-Print Network [OSTI]

for the study of single molecule protein- DNA interactions.Physics Studies in Single Molecule Biophysics ProfessorOF THE DISSERTATION Single Molecule Studies of DNA Packaging

Fuller, Derek Nathan

2008-01-01T23:59:59.000Z

394

DNA DAMAGE QUANTITATION BY ALKALINE GEL ELECTROPHORESIS.  

SciTech Connect (OSTI)

Physical and chemical agents in the environment, those used in clinical applications, or encountered during recreational exposures to sunlight, induce damages in DNA. Understanding the biological impact of these agents requires quantitation of the levels of such damages in laboratory test systems as well as in field or clinical samples. Alkaline gel electrophoresis provides a sensitive (down to {approx} a few lesions/5Mb), rapid method of direct quantitation of a wide variety of DNA damages in nanogram quantities of non-radioactive DNAs from laboratory, field, or clinical specimens, including higher plants and animals. This method stems from velocity sedimentation studies of DNA populations, and from the simple methods of agarose gel electrophoresis. Our laboratories have developed quantitative agarose gel methods, analytical descriptions of DNA migration during electrophoresis on agarose gels (1-6), and electronic imaging for accurate determinations of DNA mass (7-9). Although all these components improve sensitivity and throughput of large numbers of samples (7,8,10), a simple version using only standard molecular biology equipment allows routine analysis of DNA damages at moderate frequencies. We present here a description of the methods, as well as a brief description of the underlying principles, required for a simplified approach to quantitation of DNA damages by alkaline gel electrophoresis.

SUTHERLAND,B.M.; BENNETT,P.V.; SUTHERLAND, J.C.

2004-03-24T23:59:59.000Z

395

Laser mass spectrometry for DNA sequencing, disease diagnosis, and fingerprinting  

SciTech Connect (OSTI)

Since laser mass spectrometry has the potential for achieving very fast DNA analysis, the authors recently applied it to DNA sequencing, DNA typing for fingerprinting, and DNA screening for disease diagnosis. Two different approaches for sequencing DNA have been successfully demonstrated. One is to sequence DNA with DNA ladders produced from Snager`s enzymatic method. The other is to do direct sequencing without DNA ladders. The need for quick DNA typing for identification purposes is critical for forensic application. The preliminary results indicate laser mass spectrometry can possibly be used for rapid DNA fingerprinting applications at a much lower cost than gel electrophoresis. Population screening for certain genetic disease can be a very efficient step to reducing medical costs through prevention. Since laser mass spectrometry can provide very fast DNA analysis, the authors applied laser mass spectrometry to disease diagnosis. Clinical samples with both base deletion and point mutation have been tested with complete success.

Winston Chen, C.H.; Taranenko, N.I.; Zhu, Y.F.; Chung, C.N.; Allman, S.L.

1997-03-01T23:59:59.000Z

396

DNA fragment sizing and sorting by laser-induced fluorescence  

DOE Patents [OSTI]

A method is provided for sizing DNA fragments using high speed detection systems, such as flow cytometry to determine unique characteristics of DNA pieces from a sample. In one characterization the DNA piece is fragmented at preselected sites to produce a plurality of DNA fragments. The DNA piece or the resulting DNA fragments are treated with a dye effective to stain stoichiometrically the DNA piece or the DNA fragments. The fluorescence from the dye in the stained fragments is then examined to generate an output functionally related to the number of nucleotides in each one of the DNA fragments. In one embodiment, the intensity of the fluorescence emissions from each fragment is linearly related to the fragment length. The distribution of DNA fragment sizes forms a characterization of the DNA piece for use in forensic and research applications.

Hammond, Mark L. (Angier, NC); Jett, James H. (Los Alamos, NM); Keller, Richard A. (Los Alamos, NM); Marrone, Babetta L. (Los Alamos, NM); Martin, John C. (Los Alamos, NM)

1996-01-01T23:59:59.000Z

397

Exploring the common molecular basis for the universal DNA mutation bias: Revival of Loewdin mutation model  

SciTech Connect (OSTI)

Highlights: {yields} There exists a universal G:C {yields} A:T mutation bias in three domains of life. {yields} This universal mutation bias has not been sufficiently explained. {yields} A DNA mutation model proposed by Loewdin 40 years ago offers a common explanation. -- Abstract: Recently, numerous genome analyses revealed the existence of a universal G:C {yields} A:T mutation bias in bacteria, fungi, plants and animals. To explore the molecular basis for this mutation bias, we examined the three well-known DNA mutation models, i.e., oxidative damage model, UV-radiation damage model and CpG hypermutation model. It was revealed that these models cannot provide a sufficient explanation to the universal mutation bias. Therefore, we resorted to a DNA mutation model proposed by Loewdin 40 years ago, which was based on inter-base double proton transfers (DPT). Since DPT is a fundamental and spontaneous chemical process and occurs much more frequently within GC pairs than AT pairs, Loewdin model offers a common explanation for the observed universal mutation bias and thus has broad biological implications.

Fu, Liang-Yu [National Key Laboratory of Crop Genetic Improvement, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070 (China) [National Key Laboratory of Crop Genetic Improvement, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070 (China); Center for Bioinformatics, Huazhong Agricultural University, Wuhan 430070 (China); Wang, Guang-Zhong [State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101 (China)] [State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101 (China); Ma, Bin-Guang [Center for Bioinformatics, Huazhong Agricultural University, Wuhan 430070 (China)] [Center for Bioinformatics, Huazhong Agricultural University, Wuhan 430070 (China); Zhang, Hong-Yu, E-mail: zhy630@mail.hzau.edu.cn [National Key Laboratory of Crop Genetic Improvement, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070 (China) [National Key Laboratory of Crop Genetic Improvement, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070 (China); Center for Bioinformatics, Huazhong Agricultural University, Wuhan 430070 (China)

2011-06-10T23:59:59.000Z

398

Design of a combinatorial dna microarray for protein-dnainteraction studies  

SciTech Connect (OSTI)

Background: Discovery of precise specificity oftranscription factors is an important step on the way to understandingthe complex mechanisms of gene regulation in eukaryotes. Recently,doublestranded protein-binding microarrays were developed as apotentially scalable approach to tackle transcription factor binding siteidentification. Results: Here we present an algorithmic approach toexperimental design of a microarray that allows for testing fullspecificity of a transcription factor binding to all possible DNA bindingsites of a given length, with optimally efficient use of the array. Thisdesign is universal, works for any factor that binds a sequence motif andis not species-specific. Furthermore, simulation results show that dataproduced with the designed arrays is easier to analyze and would resultin more precise identification of binding sites. Conclusion: In thisstudy, we present a design of a double stranded DNA microarray forprotein-DNA interaction studies and show that our algorithm allowsoptimally efficient use of the arrays for this purpose. We believe such adesign will prove useful for transcription factor binding siteidentification and other biological problems.

Mintseris, Julian; Eisen, Michael B.

2006-07-07T23:59:59.000Z

399

Natural and synthetic double-stranded DNA binding studies of macrocyclic tetraamine zinc(II) complexes appended with polyaromatic groups  

Science Journals Connector (OSTI)

...?The characteristic binding mode of zinc(II) complexes of macrocyclic tetraamines (1,4,7,10-tetraazacyclododecane, cyclen) appended with one or two arylmethyl group(s) [(4-quinolyl)methyl-, 1,7-bis(4-quinolyl)...

Emiko Kikuta; Naomi Katsube; E. Kimura

1999-08-01T23:59:59.000Z

400

DNA double-strand breaks promote methylation of histone H3 on lysine 9 and transient formation of repressive chromatin  

Science Journals Connector (OSTI)

...7456 ): 50 – 54 . 11 Price BD D’Andrea AD ( 2013...Y Gursoy-Yuzugullu O Price BD ( 2012 ) The...Apochromat/1.25-N.A. oil objective (Leica...Plan APO 63×/1.4 oil M27 lens (magnification...proteins were released by heating for 5 min with 2× sample...

Marina K. Ayrapetov; Ozge Gursoy-Yuzugullu; Chang Xu; Ye Xu; Brendan D. Price

2014-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "dna double helix" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


401

Functional role of MRE11 in the repair of particulate chromium(VI)-induced DNA double strand breaks  

Science Journals Connector (OSTI)

...mast cells can behave alternatively as dangerous promoters, innocent bystanders, or...Italo Monzino, the Italian Ministry of Health, and Associazione Italiana Ricerca sul...mast cells can behave alternatively as dangerous promoters, innocent bystanders, or...

Hong Xie; Sandra S. Wise; Seme Dako; Spiros Katsifis; and John P. Wise

2006-04-15T23:59:59.000Z

402

Next Generation Neutrinoless Double -Decay Experiments Andreas Piepke, R.G. Hamish Robertson  

E-Print Network [OSTI]

of this ambitious program...". The Long Range Plan further states: "... neutrinoless double beta decay experiments

403

Sequential addition of short DNA oligos in DNA-polymerase-based synthesis reactions  

DOE Patents [OSTI]

A method of preselecting a multiplicity of DNA sequence segments that will comprise the DNA molecule of user-defined sequence, separating the DNA sequence segments temporally, and combining the multiplicity of DNA sequence segments with at least one polymerase enzyme wherein the multiplicity of DNA sequence segments join to produce the DNA molecule of user-defined sequence. Sequence segments may be of length n, where n is an odd integer. In one embodiment the length of desired hybridizing overlap is specified by the user and the sequences and the protocol for combining them are guided by computational (bioinformatics) predictions. In one embodiment sequence segments are combined from multiple reading frames to span the same region of a sequence, so that multiple desired hybridizations may occur with different overlap lengths.

Gardner, Shea N; Mariella, Jr., Raymond P; Christian, Allen T; Young, Jennifer A; Clague, David S

2013-06-25T23:59:59.000Z

404

DNA interaction with synthetic polymers in solution  

Science Journals Connector (OSTI)

The complexes in which a DNA molecule is compact and protected from the ... lipids, inactivated viral particles, polymeric micelles and synthetic polycations are used [12–23...]. The latter have important advanta...

Nina Kasyanenko; Daria Afanasieva; Boris Dribinsky; Dmitry Mukhin…

2007-08-01T23:59:59.000Z

405

Interconnecting gold islands with DNA origami  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

to integrate the bottom-up self-assembly of DNA origami with the top-down lithographic methods used to generate surface patterning. In this report we demonstrate that fixed length...

406

DNA Assembly Line for Nano-Construction  

ScienceCinema (OSTI)

Building on the idea of using DNA to link up nanoparticles scientists at Brookhaven National Lab have designed a molecular assembly line for high-precision nano-construction. Nanofabrication is essential for exploiting the unique properties of nanoparticl

Oleg Gang

2010-01-08T23:59:59.000Z

407

Linear Thermodynamics of Rodlike DNA Filtration  

E-Print Network [OSTI]

Linear thermodynamics transportation theory is employed to study filtration of rodlike DNA molecules. Using the repeated nanoarray consisting of alternate deep and shallow regions, it is demonstrated that the complex ...

Li, Zirui

408

Double pulse laser induced breakdown spectroscopy; experimental study of  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Double pulse laser induced breakdown spectroscopy; experimental study of Double pulse laser induced breakdown spectroscopy; experimental study of lead emission intensity dependence on the wavelengths and sample matrix Title Double pulse laser induced breakdown spectroscopy; experimental study of lead emission intensity dependence on the wavelengths and sample matrix Publication Type Journal Article Year of Publication 2009 Authors Piscitelli, Vincent, Mauro A. Martinez, Alberto J. Fernandez, Jhanis J. Gonzalez, Xianglei Mao, and Richard E. Russo Journal Spectrochimica Acta Part B Volume 64 Issue 2 Pagination 147-154 Date Published 02/2009 Keywords Double pulse LIBS, laser induced breakdown spectroscopy, lead Abstract Lead (Pb) emission intensity (atomic line 405.78 nm) dependence on the sample matrix (metal alloy) was studied by means of collinear double pulse (DP)-laser induced breakdown spectroscopy (LIBS). The measurement of the emission intensity produced by three different wavelength combinations (i.e. I:532 nm-II:1064 nm, I:532 nm-II:532 nm, and I:532 nm-II:355 nm) from three series of standard reference materials showed that the lead atomic line 405.78 nm emission intensity was dependent on the sample matrix for all the combination of wavelengths, however reduced dependency was found for the wavelength combination I:532 nm-II:355 nm.

409

Localized model for systems with double-exchange coupling  

Science Journals Connector (OSTI)

Magnetic spins in crystals of mixed valence can simultaneously experience two kinds of coupling: the superexchange and the double exchange. The latter coupling, first invoked by Zener and further worked out by Anderson and Hasegawa, is mediated by additional electrons or holes introduced into the system. In the present paper an effective site-spins interaction Hamiltonian for double exchange is formulated. This Hamiltonian involves ascending powers of the bilinear interaction S?i·S?j. The highest power is determined by the value of the site spin. Thus, for spin-½ the double exchange interaction looks like the ordinary Heisenberg-type coupling. Spin-1 Hamiltonian contains also a biquadratic coupling. Spin-32 includes a bicubic interaction, spin-2 a biquartic one, etc. It is argued that a localized description of systems with the double exchange is usually sufficient. The phase diagrams are entirely different from the one predicted by the semiclassical (large-spin) band theory proposed by de Gennes. The critical concentrations of the carriers are evaluated at T=0 K in the mean-field-theory approximation. An applied magnetic field is shown to have little influence on the strength of the double-exchange coupling. Spin configurations in the presence of the field are also discussed. Finally, a spin-wave theory for such systems is constructed.

M. Cieplak

1978-10-01T23:59:59.000Z

410

Systematic decomposition of the neutrinoless double beta decay operator  

E-Print Network [OSTI]

We discuss the systematic decomposition of the dimension nine neutrinoless double beta decay operator, focusing on mechanisms with potentially small contributions to neutrino mass, while being accessible at the LHC. We first provide a (d=9 tree-level) complete list of diagrams for neutrinoless double beta decay. From this list one can easily recover all previously discussed contributions to the neutrinoless double beta decay process, such as the celebrated mass mechanism or "exotics", such as contributions from left-right symmetric models, R-parity violating supersymmetry and leptoquarks. More interestingly, however, we identify a number of new possibilities which have not been discussed in the literature previously. Contact to earlier works based on a general Lorentz-invariant parametrisation of the neutrinoless double beta decay rate is made, which allows, in principle, to derive limits on all possible contributions. We furthermore discuss possible signals at the LHC for mediators leading to the short-range part of the amplitude with one specific example. The study of such contributions would gain particular importance if there were a tension between different measurements of neutrino mass such as coming from neutrinoless double beta decay and cosmology or single beta decay.

Florian Bonnet; Martin Hirsch; Toshihiko Ota; Walter Winter

2012-12-13T23:59:59.000Z

411

The beauty of DNA architecture : the design and applications in DNA nanotechnology.  

E-Print Network [OSTI]

??The mainframe of this thesis is about DNA nanotechnology in the perspectives of materials science and molecular architecture. My first project was to develop a… (more)

Wei, Diming

2009-01-01T23:59:59.000Z

412

DNA-incorporating nanomaterials in biotechnological applications  

SciTech Connect (OSTI)

The recently developed ability to controllably connect biological and inorganic objects on a molecular scale opens a new page in biomimetic methods with potential applications in biodetection, tissue engineering, targeted therapeutics and drug/gene delivery. Particularly in the biodetection arena, a rapid development of new platforms has largely been stimulated by a spectrum of novel nanomaterials with physical properties that offer efficient, sensitive and inexpensive molecular sensing. Recently, DNA-functionalized nano-objects have emerged as a new class of nanomaterials that can be controllably assembled in predesigned structures. Such DNA-based nanoscale structures might provide a new detection paradigm due to their regulated optical, electrical and magnetic responses, chemical heterogeneity and high local biomolecular concentration. The specific biorecognition DNA and its physical-chemical characteristics allows for an exploitation of DNA-functionalized nanomaterials for sensing of nucleic acids, while a broad tunability of DNA interactions permits extending their use for detection of proteins, small molecules and ions. We discuss the progress that was achieved in the last decade in the exploration of new detection methods based on DNA-incorporating nanomaterials as well as their applications to gene delivery. The comparison between various detection platforms, their sensitivity and selectivity, and specific applications are reviewed.

Stadler, A.; van der Lelie, D.; Chi, C.; Gang, O.

2010-02-01T23:59:59.000Z

413

Oligonucleotide and Long Polymeric DNA Encoding  

SciTech Connect (OSTI)

This report summarizes the work done at Lawrence Livermore National Laboratory for the Oligonucleotide and Long Polymeric DNA Encoding project, part of the Microelectronic Bioprocesses Program at DARPA. The goal of the project was to develop a process by which long (circa 10,000 base-pair) synthetic DNA molecules could be synthesized in a timely and economic manner. During construction of the long molecule, errors in DNA sequence occur during hybridization and/or the subsequent enzymatic process. The work done on this project has resulted in a novel synthesis scheme that we call the parallel pyramid synthesis protocol, the development of a suit of computational tools to minimize and quantify errors in the synthesized DNA sequence, and experimental proof of this technique. The modeling consists of three interrelated modules: the bioinformatics code which determines the specifics of parallel pyramid synthesis for a given chain of long DNA, the thermodynamics code which tracks the products of DNA hybridization and polymerase extension during the later steps in the process, and the kinetics model which examines the temporal and spatial processes during one thermocycle. Most importantly, we conducted the first successful syntheses of a gene using small starting oligomers (tetramers). The synthesized sequence, 813 base pairs long, contained a 725 base pair gene, modified green fluorescent protein (mGFP), which has been shown to be a functional gene by cloning into cells and observing its green fluorescent product.

Miller, E; Mariella Jr., R P; Christian, A T; Gardner, S N; Williams, J M

2003-11-24T23:59:59.000Z

414

When DNA Needs to Stand Up and Be Counted  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

When DNA Needs to Stand Up and Be Counted Print When DNA Needs to Stand Up and Be Counted Print DNA microarrays are small metal, glass, or silicon chips covered with patterns of short single-stranded DNA (ssDNA). These "DNA chips" are revolutionizing biotechnology, allowing scientists to identify and count many DNA sequences simultaneously. They are the enabling technology for genomic-based medicine and are a critical component of advanced diagnostic systems for medical and homeland security applications. Like digital chips, DNA chips are parallel, accurate, fast, and small. These advantages, however, can only be realized if the fragile biomolecules survive the attachment process intact. Furthermore, biomolecules must be properly oriented to perform their biological function. In other words, the DNA literally must stand up to be counted. Understanding both the attachment and orientation of DNA on gold surfaces was the goal of recent experiments performed at ALS Beamline 8.0.1 by an international collaboration of scientists.

415

When DNA Needs to Stand Up and Be Counted  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

When DNA Needs to Stand Up and Be Counted Print When DNA Needs to Stand Up and Be Counted Print DNA microarrays are small metal, glass, or silicon chips covered with patterns of short single-stranded DNA (ssDNA). These "DNA chips" are revolutionizing biotechnology, allowing scientists to identify and count many DNA sequences simultaneously. They are the enabling technology for genomic-based medicine and are a critical component of advanced diagnostic systems for medical and homeland security applications. Like digital chips, DNA chips are parallel, accurate, fast, and small. These advantages, however, can only be realized if the fragile biomolecules survive the attachment process intact. Furthermore, biomolecules must be properly oriented to perform their biological function. In other words, the DNA literally must stand up to be counted. Understanding both the attachment and orientation of DNA on gold surfaces was the goal of recent experiments performed at ALS Beamline 8.0.1 by an international collaboration of scientists.

416

Disorder in DNA-Linked Gold Nanoparticle Assemblies  

Science Journals Connector (OSTI)

We report experimental observations on the effect of disorder on the phase behavior of DNA-linked nanoparticle assemblies. Variation in DNA linker lengths results in different melting temperatures of the DNA-linked nanoparticle assemblies. We observed an unusual trend of a nonmonotonic “zigzag” pattern in the melting temperature as a function of DNA linker length. Linker DNA resulting in unequal DNA duplex lengths introduces disorder and lowers the melting temperature of the nanoparticle system. Comparison with free DNA thermodynamics shows that such an anomalous zigzag pattern does not exist for free DNA duplex melting, which suggests that the disorder introduced by unequal DNA duplex lengths results in this unusual collective behavior of DNA-linked nanoparticle assemblies.

Nolan C. Harris and Ching-Hwa Kiang

2005-07-21T23:59:59.000Z

417

Nucleotide sequence of Bacillus subtilis dnaB: a gene essential for DNA replication initiation and membrane attachment  

SciTech Connect (OSTI)

The complete nucleotide sequence of the Bacillus subtilis dnaB gene and its flanking regions was determined. The dnaB gene is essential for both replication initiation and membrane attachment of the origin region of the chromosome and plasmid pUB110. It has been known that there are two different classes (dnaBI and dnaBII) in the dnaB mutants; dnaBI is essential for both chromosome and pUB110 replication, whereas dnaBII is necessary only for chromosome replication. The nucleotide sequence revealed that dnaBI and dnaBII are two functional domains in the single dnaB gene. The mutation sites of two mutants, belonging to dnaBI and dnaBII, respectively, were also determined as substitutions of amino acids. The putative DnaB protein deduced from nucleotide sequence consists of 472 amino acids (55 kDa) with no cysteine residue. A 55-kDa polypeptide produced in an in vitro transcription-translation system was labeled with (/sup 35/S)methionine but not with (/sup 35/S)cysteine. The DnaB protein has a highly hydrophobic sequence of 20 amino acids in its N-terminal region, a possible DNA binding site, and two possible ATP binding sites. The dnaBI domain is between the DNA binding site and one of the ATP binding sites; the dnaBII domain is close to the other ATP binding site. Comparison of the amino acid sequence between the dnaB protein and those of other dna genes of Escherichia coli showed no homology, suggesting that the dnaB gene of B, subtilis may be analogous to a hitherto undiscovered gene in E. coli.

Hoshino, T.; McKenzie, T.; Schmidt, S.; Tanaka, T.; Sueoka, N.

1987-02-01T23:59:59.000Z

418

NREL: News Feature - Nation Could Double Energy Productivity  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Nation Could Double Energy Productivity Nation Could Double Energy Productivity February 7, 2013 Photo of NREL Director Dan Arvizu speaking at NREL. Enlarge image NREL Director Dan Arvizu and a blue-ribbon panel of 20 energy experts said that the United States can double its energy productivity by 2030 - and do so in ways that bolster the nation's economy. In this photo, Arvizu speaks to commercial building stakeholders at NREL. Credit: Dennis Schroeder Researchers at the U.S. Department of Energy's (DOE) National Renewable Energy Laboratory (NREL) have long understood that using energy more efficiently can be just as beneficial as finding new ways to produce energy more efficiently. On Feb. 7, NREL Director Dan Arvizu and a blue-ribbon panel of 20 energy experts drove that message home, declaring that the United States can

419

CIRCUMSTELLAR ABSORPTION IN DOUBLE DETONATION TYPE Ia SUPERNOVAE  

SciTech Connect (OSTI)

Upon formation, degenerate He core white dwarfs are surrounded by a radiative H-rich layer primarily supported by ideal gas pressure. In this Letter, we examine the effect of this H-rich layer on mass transfer in He+C/O double white dwarf binaries that will eventually merge and possibly yield a Type Ia supernova (SN Ia) in the double detonation scenario. Because its thermal profile and equation of state differ from the underlying He core, the H-rich layer is transferred stably onto the C/O white dwarf prior to the He core's tidal disruption. We find that this material is ejected from the binary system and sweeps up the surrounding interstellar medium hundreds to thousands of years before the SN Ia. The close match between the resulting circumstellar medium profiles and values inferred from recent observations of circumstellar absorption in SNe Ia gives further credence to the resurgent double detonation scenario.

Shen, Ken J. [Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA 94720 (United States); Guillochon, James [Department of Astronomy and Astrophysics, University of California, Santa Cruz, CA 95064 (United States); Foley, Ryan J., E-mail: kenshen@astro.berkeley.edu [Harvard-Smithsonian Center for Astrophysics, 60 Garden Street, Cambridge, MA 02138 (United States)

2013-06-20T23:59:59.000Z

420

Q value of the 100Mo Double-Beta Decay  

E-Print Network [OSTI]

Penning trap measurements using mixed beams of 100Mo - 100Ru and 76Ge - 76Se have been utilized to determine the double-beta decay Q-values of 100Mo and 76Ge with uncertainties less than 200 eV. The value for 76Ge, 2039.04(16) keV is in agreement with the published SMILETRAP value. The new value for 100Mo, 3034.40(17) keV is 30 times more precise than the previous literature value, sufficient for the ongoing neutrinoless double-beta decay searches in 100Mo. Moreover, the precise Q-value is used to calculate the phase-space integrals and the experimental nuclear matrix element of double-beta decay.

S. Rahaman; V. -V. Elomaa; T. Eronen; J. Hakala; A. Jokinen; J. Julin; A. Kankainen; A. Saastamoinen; J. Suhonen; C. Weber; J. Äystö

2007-12-20T23:59:59.000Z

Note: This page contains sample records for the topic "dna double helix" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


421

Self-assembled DNA Nanostructures and DNA Devices John Reif Harish Chandran Nikhil Gopalkrishnan Thomas LaBean  

E-Print Network [OSTI]

and current state of the emerging research area in the field of nanoscience that make use of synthetic DNA. They are constructed primarily of synthetic DNA. 1.2 Use of bottom-up self-assembly Construction of molecularSelf-assembled DNA Nanostructures and DNA Devices John Reif Harish Chandran Nikhil Gopalkrishnan

Reif, John H.

422

Baseline Cellular HIV DNA Load Predicts HIV DNA Decline and Residual HIV Plasma Levels during Effective Antiretroviral Therapy  

Science Journals Connector (OSTI)

...significant predictive power, but published data on its correlation...correlated with HIV DNA load decrease until...cellular HIV DNA load predicts HIV DNA...significant predictive power, but published data on its correlation...correlated with HIV DNA load decrease until...

Saverio Giuseppe Parisi; Samantha Andreis; Carlo Mengoli; Renzo Scaggiante; Roberto Ferretto; Vinicio Manfrin; Mario Cruciani; Mario Giobbia; Caterina Boldrin; Monica Basso; Massimo Andreoni; Giorgio Palł; Loredana Sarmati

2011-11-30T23:59:59.000Z

423

HANFORD DOUBLE SHELL TANK THERMAL AND SEISMIC PROJECT SEISMIC ANALYSIS OF HANFORD DOUBLE SHELL TANKS  

SciTech Connect (OSTI)

M&D Professional Services, Inc. (M&D) is under subcontract to Pacific Northwest National Laboratories (PNNL) to perform seismic analysis of the Hanford Site Double-Shell Tanks (DSTs) in support of a project entitled Double-Shell Tank (DST) Integrity Project - DST Thermal and Seismic Analyses. The original scope of the project was to complete an up-to-date comprehensive analysis of record of the DST System at Hanford in support of Tri-Party Agreement Milestone M-48-14. The work described herein was performed in support of the seismic analysis of the DSTs. The thermal and operating loads analysis of the DSTs is documented in Rinker et al. (2004). Although Milestone M-48-14 has been met, Revision I is being issued to address external review comments with emphasis on changes in the modeling of anchor bolts connecting the concrete dome and the steel primary tank. The work statement provided to M&D (PNNL 2003) required that a nonlinear soil structure interaction (SSI) analysis be performed on the DSTs. The analysis is required to include the effects of sliding interfaces and fluid sloshing (fluid-structure interaction). SSI analysis has traditionally been treated by frequency domain computer codes such as SHAKE (Schnabel, et al. 1972) and SASSI (Lysmer et al. 1999a). Such frequency domain programs are limited to the analysis of linear systems. Because of the contact surfaces, the response of the DSTs to a seismic event is inherently nonlinear and consequently outside the range of applicability of the linear frequency domain programs. That is, the nonlinear response of the DSTs to seismic excitation requires the use of a time domain code. The capabilities and limitations of the commercial time domain codes ANSYS{reg_sign} and MSC Dytran{reg_sign} for performing seismic SSI analysis of the DSTs and the methodology required to perform the detailed seismic analysis of the DSTs has been addressed in Rinker et al (2006a). On the basis of the results reported in Rinker et al. (2006a), it is concluded that time-domain SSI analysis using ANSYS{reg_sign} is justified for predicting the global response of the DSTs. The most significant difference between the current revision (Revision 1) of this report and the original issue (Revision 0) is the treatment of the anchor bolts that tie the steel dome of the primary tank to the concrete tank dome.

MACKEY TC; RINKER MW; CARPENTER BG; HENDRIX C; ABATT FG

2009-01-15T23:59:59.000Z

424

HANFORD DOUBLE SHELL TANK (DST) THERMAL & SEISMIC PROJECT SEISMIC ANALYSIS OF HANFORD DOUBLE SHELL TANKS  

SciTech Connect (OSTI)

M&D Professional Services, Inc. (M&D) is under subcontract to Pacific Northwest National Laboratory (PNNL) to perform seismic analysis of the Hanford Site double-shell tanks (DSTs) in support of a project entitled ''Double-Shell Tank (DSV Integrity Project--DST Thermal and Seismic Analyses)''. The overall scope of the project is to complete an up-to-date comprehensive analysis of record of the DST system at Hanford in support of Tri-Party Agreement Milestone M-48-14, The work described herein was performed in support of the seismic analysis of the DSTs. The thermal and operating loads analysis of the DSTs is documented in Rinker et al. (2004). The work statement provided to M&D (PNNL 2003) required that the seismic analysis of the DSTs assess the impacts of potentially non-conservative assumptions in previous analyses and account for the additional soil mass due to the as-found soil density increase, the effects of material degradation, additional thermal profiles applied to the full structure including the soil-structure response with the footings, the non-rigid (low frequency) response of the tank roof, the asymmetric seismic-induced soil loading, the structural discontinuity between the concrete tank wall and the support footing and the sloshing of the tank waste. The seismic analysis considers the interaction of the tank with the surrounding soil and the effects of the primary tank contents. The DSTs and the surrounding soil are modeled as a system of finite elements. The depth and width of the soil incorporated into the analysis model are sufficient to obtain appropriately accurate analytical results. The analyses required to support the work statement differ from previous analysis of the DSTs in that the soil-structure interaction (SSI) model includes several (nonlinear) contact surfaces in the tank structure, and the contained waste must be modeled explicitly in order to capture the fluid-structure interaction behavior between the primary tank and contained waste.

MACKEY, T.C.

2006-03-17T23:59:59.000Z

425

Implementation of Double-Waist Chicane Optics in SPEAR3  

SciTech Connect (OSTI)

The SPEAR3 accelerator upgrade opened up two 7.6m racetrack straights in the magnet lattice. In one of these straights, we recently added a magnetic chicane to separate two insertion device (ID) beam lines by 10mrad. A quadrupole triplet in the center creates a ''double focus'' optics with {beta}y = 1.6m at the middle of each ID, hence the term ''double-waist chicane''. The new optics also reduced {beta}y in the four matching straights adjacent to the racetrack straights to 2.5m. In this paper, we outline design features of the optics and physical implementation of the lattice.

Corbett, J.; Cornacchia, M.; Dao, T.; Dell'Orco, D.; Rafael, F.; Harrington, D.; Hettel, R.; Huang, X.; Nosochkov, Y.; Rabedeau, T.; Rarback, H.; Ringwall, A.; Safranek,; Scott, B.; Sebek, J.; Tanabe, J.; Terebilo, A.; Wermelskirchen, C.; Widmeyer, M.; /SLAC, SSRL; Yoon, Moohyun; /POSTECH

2006-08-16T23:59:59.000Z

426

The fabrication of double shell targets for NOVA  

SciTech Connect (OSTI)

At scales appropriate to the National Ignition Facility, calculations indicate double shell capsules reach ignition conditions with room temperature fuel. Los Alamos is laying the groundwork for these double shell ignition targets with a preliminary campaign on Nova. The target design features a 260 {micro}m O.D. inner glass capsule with an I.D. of approximately 200 {micro}m, containing deuterium gas, centered inside a machined polymer shell. The central capsule was supported either with a 30 mg/cc foam or with two polymer films, each less than 0.1 {micro}m in thickness. The materials, machining, assembly, and characterization of the targets are discussed.

Duke, J.R.; Elliott, N.E.; Moore, J.E.; Gomez, V.M.; Manzanares, R.; Rivera, G.; Watt, R.; Varnum, W.S.; Gobby, P.L. [Los Alamos National Lab., NM (United States)

1999-03-01T23:59:59.000Z

427

Progress toward Ignition with Noncryogenic Double-Shell Capsules  

SciTech Connect (OSTI)

Inertial confinement fusion implosions using capsules with two concentric shells separated by a low density region (double shells) are reported which closely follow one dimensional (1D) radiatively driven hydrodynamics simulations. Capsule designs which mitigate Au M -band radiation asymmetries appear to correspond more closely to 1D simulations than targets lacking mitigation of hohlraum drive M -band nonuniformities. One capsule design achieves over 50% of the unperturbed 1D calculated yield at a convergence ratio of 25.5, comparable to that of a double-shell design for an ignition capsule at the National Ignition Facility. (c) 2000 The American Physical Society.

Varnum, W. S.; Delamater, N. D.; Evans, S. C.; Gobby, P. L.; Moore, J. E.; Wallace, J. M.; Watt, R. G.; Colvin, J. D.; Turner, R.; Glebov, V. (and others) [and others

2000-05-29T23:59:59.000Z

428

Microconvection effects at double?diffusive gradient zone boundaries  

Science Journals Connector (OSTI)

Microconvection in double?diffusive gradient zones is predicted to occur near the zone boundaries because of effects of boundary undulation and temperature modulation caused by impinging thermals in adjacent convecting zones. The equations that govern convective motion in a double?diffusive horizontal slab are solved for boundary conditions that incorporate these effects. Solution of these equations predicts a weakened salinity gradient near the gradient zone boundary between the rising thermals. When the salinity gradient is too weak instability occurs taking the form of descending plumes which are seen in experiments.

John R. Hull; Yojana Katti

1988-01-01T23:59:59.000Z

429

Empirical Survey of Neutrinoless Double Beta Decay Matrix Elements  

E-Print Network [OSTI]

Neutrinoless double beta decay has been the subject of intensive theoretical work as it represents the only practical approach to discovering whether neutrinos are Majorana particles or not, and whether lepton number is a conserved quantum number. Available calculations of matrix elements and phase-space factors are reviewed from the perspective of a future large-scale experimental search for neutrinoless double beta decay. Somewhat unexpectedly, a uniform inverse correlation between phase space and the square of the nuclear matrix element emerges. As a consequence, no isotope is either favored or disfavored; all have qualitatively the same decay rate per unit mass for any given value of the Majorana mass.

R. G. H. Robertson

2013-01-07T23:59:59.000Z

430

R2oDNA Designer: Computational Design of Biologically Neutral Synthetic DNA Sequences  

Science Journals Connector (OSTI)

R2oDNA Designer: Computational Design of Biologically Neutral Synthetic DNA Sequences ... As the cost of whole gene synthesis is decreasing, whole genome synthesis at the other end of the spectrum has expanded our horizons to the prospect of fully engineered synthetic cells. ...

Arturo Casini; Georgia Christodoulou; Paul S. Freemont; Geoff S. Baldwin; Tom Ellis; James T. MacDonald

2014-01-31T23:59:59.000Z

431

Meta-DNA: Synthetic Biology via DNA Nanostructures and Hybridization Reactions  

E-Print Network [OSTI]

Meta-DNA: Synthetic Biology via DNA Nanostructures and Hybridization Reactions Harish Chandran University, Durham NC email: reif@cs.duke.edu Abstract The goal of synthetic biology is to design and assemble synthetic systems that mimic bio- logical systems. One of the most fundamental challenge

Reif, John H.

432

Two DNA methyltransferases from murine erythroleukemia cells: purification, sequence specificity, and mode of interaction with DNA  

Science Journals Connector (OSTI)

...synthetic polydeoxyribonucleotides Activity DNA DNA Substrate MeTase I MeTase II Poly(dG-dC) 2.1 2.4 Poly(dG-dC,dmSC)* 70.1 105.4 Poly(dC) 0.003 0.001 Poly(dC,dA) 0.003 0.001 A...

T H Bestor; V M Ingram

1983-01-01T23:59:59.000Z

433

Double Shell Tank AY-102 Radioactive Waste Leak Investigation  

SciTech Connect (OSTI)

PowerPoint. The objectives of this presentation are to: Describe Effort to Determine Whether Tank AY-102 Leaked; Review Probable Causes of the Tank AY-102 Leak; and, Discuss Influence of Leak on Hanford’s Double-Shell Tank Integrity Program.

Washenfelder, Dennis J.

2014-04-10T23:59:59.000Z

434

Multimedia classification and event detection using double fusion  

Science Journals Connector (OSTI)

Multimedia Event Detection(MED) is a multimedia retrieval task with the goal of finding videos of a particular event in video archives, given example videos and event descriptions; different from MED, multimedia classification is a task that classifies ... Keywords: Double fusion, Early fusion, Feature combination, Late fusion, Multimedia event detection

Zhen-Zhong Lan, Lei Bao, Shoou-I Yu, Wei Liu, Alexander G. Hauptmann

2014-07-01T23:59:59.000Z

435

Effects of New Gravitational Interactions on Neutrinoless Double Beta Decay  

E-Print Network [OSTI]

It has recently been proposed that violations of Lorentz invariance or violations of the equivalence principle can be constrained from the non-observation of neutrinoless double beta decay. We generalize this analysis to all possible new gravitational interactions and discuss briefly the constraints for different cases.

H. V. Klapdor--Kleingrothaus; H. Päs; U. Sarkar

2000-02-20T23:59:59.000Z

436

Double-Sided Cooling Design for Novel Planar Module  

SciTech Connect (OSTI)

A novel packaging structure for medium power modules featuring power semiconductor switches sandwiched between two symmetric substrates that fulfill electrical conduction and insulation functions is presented. Large bonding areas between dies and substrates allow this packaging technology to offer significant improvements in electrical, thermal performance. Double-sided cooling system was dedicatedly analyzed and designed for different applications.

Ning, Puqi [ORNL; Liang, Zhenxian [ORNL; Marlino, Laura D [ORNL; Wang, Fei [ORNL

2013-01-01T23:59:59.000Z

437

Circuit QED in a double quantum dot system  

SciTech Connect (OSTI)

Strong coupling peculiar feature is demonstrated in a coupled qubit-resonator system consisting of a GaAs double quantum dot and a coplanar waveguide resonator. Qubit-resonator coupling strength (g and the decoherence rate ? are directly derived from the experiment, assuring a strong coupling condition (g/? ? 2)

Toida, Hiraku; Nakajima, Takashi; Komiyama, Susumu [Department of Basic Science, University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902 (Japan)

2013-12-04T23:59:59.000Z

438

Neutrinoless Double Beta Decay with R-parity Violation  

E-Print Network [OSTI]

We consider recently observed neutrinoless double beta decay in the context of the minimal supersymmetric standard model with R-parity violating couplings lambda^{'}. We observe that most of the current experimental bounds on the R-parity violating couplings do not exclude the possibility that the neutrinoless double beta decay is caused by R-parity violation. But if we consider K-bar{K} oscillation, we observe that we have to make the R-parity violating couplings generation-dependent to accomodate with the observed neutrinoless double beta decay. And furthermore, we need some mechanism to cancel the contribution to K-bar{K} mixing from a large R-parity violating coupling. We realized this cancellation by assuming that the first- and the second- generation of quark sector do not couple with the first-generation lepton sector by R-parity violating couplings except the term W=lambda_{111}^{'} L_{1} Q_{1} D_{1}^{c}, which is responsible for the observed neutrinoless double beta decay.

Yosuke Uehara

2002-06-26T23:59:59.000Z

439

Size-consistent Brueckner theory limited to double substitutions  

Science Journals Connector (OSTI)

A size-consistent set of equations for electron correlation which are limited to double substitutions, based on Brueckner orbitals, is discussed. Called BD theory, it is shown that at fifth order of perturbation theory, BD incorporates more terms than CCSD and QCISD. The simplicity of the equations leads to an elegant gradient theory. Preliminary applications are reported.

Nicholas C. Handy; John A. Pople; Martin Head-Gordon; Krishnan Raghavachari; Gary W. Trucks

1989-01-01T23:59:59.000Z

440

Neutrinoless double beta decay mediated by the neutrino magnetic moment  

E-Print Network [OSTI]

We present a new channel of the neutrinoless double beta decay. In this scenario neutrinos not only oscillate inside the nucleus but also interact with an external non-uniform magnetic field. We assume that the field rotates about the direction of motion of the neutrino and show, that for a certain speed of rotation the half-life of the $0\

Marek Gó?d?; Wies?aw A. Kami?ski

2014-11-07T23:59:59.000Z

Note: This page contains sample records for the topic "dna double helix" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


441

Generator Coordinate Method framework for Double Beta Decay  

E-Print Network [OSTI]

We propose a consistent prescription for the derivation of the particle number and angular momentum projected QRPA (PQRPA) equation in the Generator Coordinate Method (GCM) framework for calculation of NME's of double-beta decay of axially deformed nuclei. We derive closed formulae for the calculation of excitation energies and wave functions of the intermediate nucleus.

Andrzej Bobyk; Wies?aw A. Kami?ski

2014-11-10T23:59:59.000Z

442

Double Gamow-Teller matrix elements in the germanium region  

Science Journals Connector (OSTI)

The matrix elements involved in the double-beta-decay process for the nuclei Ge76 and Se82 are calculated in terms of the variational wave functions resulting from realistic effective interactions operating in the unrestricted (2p1/2, 2p3/2, 1f5/2, 1g9/2)?,? configuration space.

S. K. Sharma, G. Mukherjee, and P. K. Rath

1990-03-01T23:59:59.000Z

443

Neutrinoless double-beta decay and seesaw mechanism  

E-Print Network [OSTI]

From the standard seesaw mechanism of neutrino mass generation, which is based on the assumption that the lepton number is violated at a large (~10exp(+15) GeV) scale, follows that the neutrinoless double-beta decay is ruled by the Majorana neutrino mass mechanism. Within this notion, for the inverted neutrino-mass hierarchy we derive allowed ranges of half-lives of the neutrinoless double-beta decay for nuclei of experimental interest with different sets of nuclear matrix elements. The present-day results of the calculation of the neutrinoless double-beta decay nuclear matrix elements are briefly discussed. We argue that if neutrinoless double-beta decay will be observed in future experiments sensitive to the effective Majorana mass in the inverted mass hierarchy region, a comparison of the derived ranges with measured half-lives will allow us to probe the standard seesaw mechanism assuming that future cosmological data will establish the sum of neutrino masses to be about 0.2 eV.

Samoil M. Bilenky; Amand Faessler; Walter Potzel; Fedor Simkovic

2011-04-11T23:59:59.000Z

444

Congenital Duodenal Obstruction and Double-Bubble Sign  

Science Journals Connector (OSTI)

Figure 1. A 1080-g girl was born at a gestational age of 29 weeks because of premature rupture of membranes. Enteral feeding was not tolerated. The infant's condition deteriorated, with nonbilious vomiting and a lack of stools. Abdominal radiography showed the double-bubble sign.

Correia-Pinto J.; Ribeiro A.

2014-09-11T23:59:59.000Z

445

Double Beta Decay and the Absolute Neutrino Mass Scale  

E-Print Network [OSTI]

After a short review of the current status of three-neutrino mixing, the implications for the values of neutrino masses are discussed. The bounds on the absolute scale of neutrino masses from Tritium beta-decay and cosmological data are reviewed. Finally, we discuss the implications of three-neutrino mixing for neutrinoless double-beta decay.

Carlo Giunti

2003-08-20T23:59:59.000Z

446

Neutrinoless double beta decay in the microscopic interacting boson model  

Science Journals Connector (OSTI)

The results of a calculation of the nuclear matrix elements for neutrinoless double beta decay in the closure approximation in several nuclei within the framework of the microscopic interacting boson model (IBM?2) are presented and compared with those calculated in the shell model (SM) and quasiparticle random phase approximation (QRPA).

F. Iachello

2009-01-01T23:59:59.000Z

447

New Advances in Neutrinoless Double Beta Decay Matrix Elements  

Science Journals Connector (OSTI)

We present the matrix elements necessary to evaluate the half?life of some neutrinoless double beta decay candidates in the framework of the microscopic interacting boson model (IBM). We compare our results with those from other models and extract some simple features of the calculations.

José Barea Muńoz

2010-01-01T23:59:59.000Z

448

Research Note Microsatellite Variation of Double-Crested Cormorant  

E-Print Network [OSTI]

Environmental Impact Statement on double- crested cormorant management allowing more flexibility in control-crested cormorants roosting in the lower Mis- sissippi Valley appear to have minimal negative impact on sport fisheries, they may have a significant impact on commercial aquaculture production in this region (Glahn

Green, Clay - Department of Biology, Texas State University

449

Neutrinoless double beta decay mediated by the neutrino magnetic moment  

E-Print Network [OSTI]

We present a new channel of the neutrinoless double beta decay. In this scenario neutrinos not only oscillate inside the nucleus but also interact with an external non-uniform magnetic field. We assume that the field rotates about the direction of motion of the neutrino and show, that for a certain speed of rotation the half-life of the $0\

Gó?d?, Marek

2014-01-01T23:59:59.000Z

450

Optical selection and collection of DNA fragments  

DOE Patents [OSTI]

Optical selection and collection of DNA fragments. The present invention includes the optical selection and collection of large (>.mu.g) quantities of clonable, chromosome-specific DNA from a sample of chromosomes. Chromosome selection is based on selective, irreversible photoinactivation of unwanted chromosomal DNA. Although more general procedures may be envisioned, the invention is demonstrated by processing chromosomes in a conventional flow cytometry apparatus, but where no droplets are generated. All chromosomes in the sample are first stained with at least one fluorescent analytic dye and bonded to a photochemically active species which can render chromosomal DNA unclonable if activated. After passing through analyzing light beam(s), unwanted chromosomes are irradiated using light which is absorbed by the photochemically active species, thereby causing photoinactivation. As desired chromosomes pass this photoinactivation point, the inactivating light source is deflected by an optical modulator; hence, desired chromosomes are not photoinactivated and remain clonable. The selection and photoinactivation processes take place on a microsecond timescale. By eliminating droplet formation, chromosome selection rates 50 times greater than those possible with conventional chromosome sorters may be obtained. Thus, usable quantities of clonable DNA from any source thereof may be collected.

Roslaniec, Mary C. (Los Alamos, NM); Martin, John C. (Los Alamos, NM); Jett, James H. (Los Alamos, NM); Cram, L. Scott (Los Alamos, NM)

1998-01-01T23:59:59.000Z

451

DNA nanotechnology: understanding and optimisation through simulation  

E-Print Network [OSTI]

DNA nanotechnology promises to provide controllable self-assembly on the nanoscale, allowing for the design of static structures, dynamic machines and computational architectures. In this article I review the state-of-the art of DNA nanotechnology, highlighting the need for a more detailed understanding of the key processes, both in terms of theoretical modelling and experimental characterisation. I then consider coarse-grained models of DNA, mesoscale descriptions that have the potential to provide great insight into the operation of DNA nanotechnology if they are well designed. In particular, I discuss a number of nanotechnological systems that have been studied with oxDNA, a recently developed coarse-grained model, highlighting the subtle interplay of kinetic, thermodynamic and mechanical factors that can determine behaviour. Finally, new results highlighting the importance of mechanical tension in the operation of a two-footed walker are presented, demonstrating that recovery from an unintended `overstepped' configuration can be accelerated by three to four orders of magnitude by application of a moderate tension to the walker's track. More generally, the walker illustrates the possibility of biasing strand-displacement processes to affect the overall rate.

Thomas E. Ouldridge

2014-11-07T23:59:59.000Z

452

Boron Neutron Capture Therapty (BNCT) in an Oral Precancer Model: Therapeutic Benefits and Potential Toxicity of a Double Application of BNCT with a Six-Week Interval  

SciTech Connect (OSTI)

Given the clinical relevance of locoregional recurrences in head and neck cancer, we developed a novel experimental model of premalignant tissue in the hamster cheek pouch for long-term studies and demonstrated the partial inhibitory effect of a single application of Boron Neutron Capture Therapy (BNCT) on tumor development from premalignant tissue. The aim of the present study was to evaluate the effect of a double application of BNCT with a 6 week interval in terms of inhibitory effect on tumor development, toxicity and DNA synthesis. We performed a double application, 6 weeks apart, of (1) BNCT mediated by boronophenylalanine (BPA-BNCT); (2) BNCT mediated by the combined application of decahydrodecaborate (GB-10) and BPA [(GB-10 + BPA)-BNCT] or (3) beam-only, at RA-3 nuclear reactor and followed the animals for 8 months. The control group was cancerized and sham-irradiated. BPA-BNCT, (GB- 10 + BPA)-BNCT and beam-only induced a reduction in tumor development from premalignant tissue that persisted until 8, 3, and 2 months respectively. An early maximum inhibition of 100% was observed for all 3 protocols. No normal tissue radiotoxicity was detected. Reversible mucositis was observed in premalignant tissue, peaking at 1 week and resolving by the third week after each irradiation. Mucositis after the second application was not exacerbated by the first application. DNA synthesis was significantly reduced in premalignant tissue 8 months post-BNCT. A double application of BPA-BNCT and (GB-10 + BPA)-BNCT, 6 weeks apart, could be used therapeutically at no additional cost in terms of radiotoxicity in normal and dose-limiting tissues.

Andrea Monti Hughes; Emiliano C.C. Pozzi; Elisa M. Heber; Silvia Thorp; Marcelo Miller; Maria E. Itoiz; Romina F. Aromando; Ana J. Molinari; Marcela A. Garabalino; David W. Nigg; Veronica A. Trivillin; Amanda E. Schwint

2011-11-01T23:59:59.000Z

453

Double Macdonald polynomials as the stable limit of Macdonald superpolynomials  

E-Print Network [OSTI]

Macdonald superpolynomials provide a remarkably rich generalization of the usual Macdonald polynomials. The starting point of this work is the observation of a previously unnoticed stability property of the Macdonald superpolynomials when the fermionic sector m is sufficiently large: their decomposition in the monomial basis is then independent of m. These stable superpolynomials are readily mapped into bisymmetric polynomials, an operation that spoils the ring structure but drastically simplifies the associated vector space. Our main result is a factorization of the (stable) bisymmetric Macdonald polynomials, called double Macdonald polynomials and indexed by pairs of partitions, into a product of Macdonald polynomials (albeit subject to non-trivial plethystic transformations). As an off-shoot, we note that, after multiplication by a t-Vandermonde determinant, this provides explicit formulas for a large class of Macdonald polynomials with prescribed symmetry. The factorization of the double Macdonald polynomials leads immediately to the generalization of basically every elementary properties of the Macdonald polynomials to the double case (norm, kernel, duality, positivity, etc). When lifted back to superspace, this validates various previously formulated conjectures in the stable regime. The q,t-Kostka coefficients associated to the double Macdonald polynomials are shown to be q,t-analogs of the dimensions of the irreducible representations of the hyperoctahedral group B_n. Moreover, a Nabla operator on the double Macdonald polynomials is defined and its action on a certain bisymmetric Schur function can be interpreted as the Frobenius series of a bigraded module of dimension (2n+1)^n, a formula again characteristic of the Coxeter group of type B_n. Finally, as a side result, we obtain a simple identity involving products of four Littlewood-Richardson coefficients.

O. Blondeau-Fournier; L. Lapointe; P. Mathieu

2013-04-08T23:59:59.000Z

454

Industrial mixing techniques for Hanford double-shell tanks  

SciTech Connect (OSTI)

Jet mixer pumps are currently the baseline technology for sludge mobilization and mixing in one-million gallon double-shell tanks at the Hanford and Savannah River Sites. Improvements to the baseline jet mixer pump technology are sought because jet mixer pumps have moving parts that may fail or require maintenance. Moreover, jet mixers are relatively expensive, they heat the waste, and, in some cases, may not mobilize enough of the sludge. This report documents a thorough literature search for commercially available applicable mixing technologies that could be used for double-shell tank sludge mobilization and mixing. Textbooks, research articles, conference proceedings, mixing experts, and the Thomas Register were consulted to identify applicable technologies. While there are many commercial methods that could be used to mobilize sludge or mix the contents of a one-million gallon tank, few will work given the geometrical constraints (e.g., the mixer must fit through a 1.07-m-diameter riser) or the tank waste properties (e.g., the sludge has such a high yield stress that it generally does not flow under its own weight). Pulsed fluid jets and submersible Flygt mixers have already been identified at Hanford and Savannah River Sites for double-shell tank mixing applications. While these mixing technologies may not be applicable for double-shell tanks that have a thick sludge layer at the bottom (since too many of these mixers would need to be installed to mobilize most of the sludge), they may have applications in tanks that do not have a settled solids layer. Retrieval projects at Hanford and other U.S. Department of Energy sites are currently evaluating the effectiveness of these mixing techniques for tank waste applications. The literature search did not reveal any previously unknown technologies that should be considered for sludge mobilization and mixing in one-million gallon double-shell tanks.

Daymo, E.A.

1997-09-01T23:59:59.000Z

455

Computationally Optimised DNA Assembly of synthetic genes  

Science Journals Connector (OSTI)

Gene synthesis is hampered by two obstacles: improper assembly of oligonucleotides; oligonucleotide defects incurred during chemical synthesis. To overcome the first problem, we describe the employment of a Computationally Optimised DNA Assembly (CODA) algorithm that uses the degeneracy of the genetic code to design overlapping oligonucleotides with thermodynamic properties for self-assembly into a single, linear, DNA product. To address the second problem, we describe a hierarchical assembly strategy that reduces the incorporation of defective oligonucleotides into full-length gene constructs. The CODA algorithm and these biological methods enable fast, simple and reliable assemblies of sequence-correct full-length genes.

Liza S.Z. Larsen; Christopher D. Wassman; G. Wesley Hatfield; Richard H. Lathrop

2008-01-01T23:59:59.000Z

456

Proofreading RNA: Structure of RNA Polymerase II's Backtracked State  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Proofreading RNA: Structure of RNA Polymerase II's Backtracked State Print Proofreading RNA: Structure of RNA Polymerase II's Backtracked State Print Proofreading Ensures Functional Proteins Genes encoded in DNA are made up of nucleotides wound into a double helix of complementary nucleotide, or base, pairs. Converting these "instructions" into complementary strands of RNA is termed transcription, and is the first of two steps in expressing genes, or turning them into proteins. Accurate transcription is crucial, because if even 1 in 100,000 bases is transcribed incorrectly it can lead to a mutated, nonfunctional protein, and possibly cell death. RNA is a template for translating nucleotides into amino acids, which are combined and folded in a prescribed manner to form proteins, enzymes, etc. To transcribe DNA to RNA, an enzyme moves along one half of an unwound DNA helix, adding nucleotides to a nascent RNA strand. One such enzyme, pol II, transcribes portions of DNA that specifically encode proteins into messenger RNA. When an incorrect base is attached to a growing RNA chain, the RNA-DNA helix distorts. Pol II shifts into a "backtracked" state and stalls, signalling the need for the mismatched nucleotide to be removed from the strand. This proofreading function plays an important role in minimizing transcription errors, speeding up protein production, and ensuring accuracy in the transition of the genetic code to the proteins that allow our bodies to function. By understanding the structure of pol II in the backtracked state, researchers can better understand this corrective function of the enzyme.

457

Proofreading RNA: Structure of RNA Polymerase II's Backtracked State  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Proofreading RNA: Structure of RNA Polymerase II's Backtracked State Print Proofreading RNA: Structure of RNA Polymerase II's Backtracked State Print Proofreading Ensures Functional Proteins Genes encoded in DNA are made up of nucleotides wound into a double helix of complementary nucleotide, or base, pairs. Converting these "instructions" into complementary strands of RNA is termed transcription, and is the first of two steps in expressing genes, or turning them into proteins. Accurate transcription is crucial, because if even 1 in 100,000 bases is transcribed incorrectly it can lead to a mutated, nonfunctional protein, and possibly cell death. RNA is a template for translating nucleotides into amino acids, which are combined and folded in a prescribed manner to form proteins, enzymes, etc. To transcribe DNA to RNA, an enzyme moves along one half of an unwound DNA helix, adding nucleotides to a nascent RNA strand. One such enzyme, pol II, transcribes portions of DNA that specifically encode proteins into messenger RNA. When an incorrect base is attached to a growing RNA chain, the RNA-DNA helix distorts. Pol II shifts into a "backtracked" state and stalls, signalling the need for the mismatched nucleotide to be removed from the strand. This proofreading function plays an important role in minimizing transcription errors, speeding up protein production, and ensuring accuracy in the transition of the genetic code to the proteins that allow our bodies to function. By understanding the structure of pol II in the backtracked state, researchers can better understand this corrective function of the enzyme.

458

Altering the Electrostatic Potential in the Major Groove: Thermodynamic and Structural Characterization of 7-Deaza-2;#8242;-deoxyadenosine:dT Base Pairing in DNA  

SciTech Connect (OSTI)

As part of an ongoing effort to explore the effect of major groove electrostatics on the thermodynamic stability and structure of DNA, a 7-deaza-2'-deoxyadenosine:dT (7-deaza-dA:dT) base pair in the Dickerson-Drew dodecamer (DDD) was studied. The removal of the electronegative N7 atom on dA and the replacement with an electropositive C-H in the major groove was expected to have a significant effect on major groove electrostatics. The structure of the 7-deaza-dA:dT base pair was determined at 1.1 {angstrom} resolution in the presence of Mg{sup 2+}. The 7-deaza-dA, which is isosteric for dA, had minimal effect on the base pairing geometry and the conformation of the DDD in the crystalline state. There was no major groove cation association with the 7-deaza-dA heterocycle. In solution, circular dichroism showed a positive Cotton effect centered at 280 nm and a negative Cotton effect centered at 250 nm that were characteristic of a right-handed helix in the B-conformation. However, temperature-dependent NMR studies showed increased exchange between the thymine N3 imino proton of the 7-deaza-dA:dT base pair and water, suggesting reduced stacking interactions and an increased rate of base pair opening. This correlated with the observed thermodynamic destabilization of the 7-deaza-dA modified duplex relative to the DDD. A combination of UV melting and differential scanning calorimetry experiments were conducted to evaluate the relative contributions of enthalpy and entropy in the thermodynamic destabilization of the DDD. The most significant contribution arose from an unfavorable enthalpy term, which probably results from less favorable stacking interactions in the modified duplex, which was accompanied by a significant reduction in the release of water and cations from the 7-deaza-dA modified DNA.

Kowal, Ewa A.; Ganguly, Manjori; Pallan, Pradeep S.; Marky, Luis A.; Gold, Barry; Egli, Martin; Stone, Michael P. (Pitt); (Vanderbilt); (Nebraska-Med)

2012-02-15T23:59:59.000Z

459

E-Print Network 3.0 - amplitude double layers Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Abstract --This paper deals a double layer and a single layer Flux-Switching Permanent Magnet (FSPM) motors for a fault Summary: Abstract --This paper deals a double layer and a...

460

The effect of weak magnetism and induced pseudoscalar coupling in neutrinoless double-beta decay  

Science Journals Connector (OSTI)

In calculating the amplitude of the majorana neutrino-mass mechanism of neutrinoless double-beta decay (0???-decay), several approximations of the...pn-rqrpa) for all nuclei undergoing double-beta decay in the re...

G. Pantis; F. Šimkovic

2000-07-01T23:59:59.000Z

Note: This page contains sample records for the topic "dna double helix" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


461

Growth Conditions of Double-Walled Carbon Nanotubes in Arc Discharge  

Science Journals Connector (OSTI)

Growth Conditions of Double-Walled Carbon Nanotubes in Arc Discharge ... Preparation conditions for large-scale synthesis of double-walled carbon nanotubes (DWCNTs) by using electric arc discharge were examined. ...

Yahachi Saito; Takanori Nakahira; Sashiro Uemura

2003-01-04T23:59:59.000Z

462

Market Power in Emissions Trading Markets Ruled by a Multiple Unit Double Auction: Further Experimental Evidence  

Science Journals Connector (OSTI)

We discuss selected methodological problems of previous Double Auction (DA) experiments and test the hypothesis that a Multiple Unit Double Auction (MUDA) is able to prevent market power in an emissions trading m...

Bodo Sturm

2008-08-01T23:59:59.000Z

463

Resistance effect of electric double layer on liquid flow in microchannel  

Science Journals Connector (OSTI)

Poisson-Boltzmann equation for EDL (electric double layer) and Navier-Stokes equation for liquid flows were numerically solved to investigate resistance effect of electric double layer on liquid flow in microchan...

Lei Gong ??; Jian-kang Wu Doctor ???

2006-10-01T23:59:59.000Z

464

SciTech Connect: Search for Neutrinoless Double-Beta Decay in...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Search for Neutrinoless Double-Beta Decay in 136Xe with EXO-200 Citation Details In-Document Search Title: Search for Neutrinoless Double-Beta Decay in 136Xe with EXO-200...

465

Neutrinoless double-$?$ decay of ${}^{82}$Se in the shell model: beyond closure approximation  

E-Print Network [OSTI]

We recently proposed a new method to calculate the standard nuclear matrix elements for neutrinoless double-$\\beta$ decay ($0\

R. A. Sen'kov; M. Horoi; B. A. Brown

2014-02-21T23:59:59.000Z

466

A measurement of the 2 neutrino double beta decay rate of 130Te in the CUORICINO experiment  

E-Print Network [OSTI]

1.2.2 Neutrinoless double beta decay . . . . . 1.2.3 NuclearEvidence for Neutrinoless Double Beta Decay, Mod. Phys.on ’Evidence for Neutrinoless Double Beta Decay’, Mod. Phys.

Kogler, Laura Katherine

2011-01-01T23:59:59.000Z

467

A measurement of the 2 neutrino double beta decay rate of Te-130 in the CUORICINO experiment  

E-Print Network [OSTI]

1.2.2 Neutrinoless double beta decay . . . . . 1.2.3 NuclearEvidence for Neutrinoless Double Beta Decay, Mod. Phys.on ’Evidence for Neutrinoless Double Beta Decay’, Mod. Phys.

Kogler, Laura

2012-01-01T23:59:59.000Z

468

Facile Dimer Synthesis for DNA-Binding Polyamide Ligands  

Science Journals Connector (OSTI)

Pyrrole-imidazole polyamide ligands are highly sequence specific synthetic DNA-binding ligands that bind with high affinity. ... Regulation of gene expression by synthetic DNA-binding ligands ...

Modi Wetzler; David E. Wemmer

2010-07-13T23:59:59.000Z

469

International congress on DNA damage and repair: Book of abstracts  

SciTech Connect (OSTI)

This document contains the abstracts of 105 papers presented at the Congress. Topics covered include the Escherichia coli nucleotide excision repair system, DNA repair in malignant transformations, defective DNA repair, and gene regulation. (TEM)

Not Available

1987-01-01T23:59:59.000Z

470

Scaling down DNA circuits with competitive neural networks  

Science Journals Connector (OSTI)

...only. It is straightforward to enforce competition in synthetic DNA circuits, because strands with common domains can easily...have presented two implementations of the WTA effect in synthetic DNA systems. While they both use autocatalysis and competition...

2013-01-01T23:59:59.000Z

471

De Novo DNA Synthesis Using Single-Molecule PCR  

Science Journals Connector (OSTI)

The broad availability of synthetic DNA oligonucleotides enabled the development of many powerful applications in biotechnology. Longer synthetic DNA molecules and libraries (generated from assembly of...1–7). Th...

Tuval Ben Yehezkel; Gregory Linshiz; Ehud Shapiro

2012-01-01T23:59:59.000Z

472

Nuclear DNA content in the genus Helianthus L. (sunflower)  

E-Print Network [OSTI]

. 1974. Genome size and the proportion of repeated nucleotide sequence DNA in plants. Biochem. Genet. 12: 257-269. Geber, G. and G. Hasibeder. 1980. Cytophotometrische Bestimmung von DNA-Nengen: Vergleich einer neuen DAPI-Fluoreszenz Method mit...

Sims, Lynne Eileen

2012-06-07T23:59:59.000Z

473

Studies of Enzymes in Mitochondrial DNA Precursor Synthesis  

E-Print Network [OSTI]

Studies of Enzymes in Mitochondrial DNA Precursor Synthesis Regulatory Mechanisms for Human;Studies of Enzymes in Mitochondrial DNA Precursor Synthesis - Regulatory Mechanisms for Human Thymidine Kinase 2 and Deoxyguanosine Kinase Abstract As important enzymes in mitochondrial nucleotide salvage

474

DNA Origami with Complex Curvatures in Three-Dimensional Space  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

DNA Origami with Complex Curvatures in Three-Dimensional Space Authors: Han, D., Pal, S., Nangreave, J., Deng, Z., Liu, Y., and Yan, H. Title: DNA Origami with Complex Curvatures...

475

A DNA damage checkpoint response in telomere-initiated senescence  

Science Journals Connector (OSTI)

... array) or triplicate (tiling path) onto 3D-link activated slides (Motorola) using a MicroGrid II arrayer (BioRobotics). DNA labelling, hybridization and analysisDNA labelling and hybridization were essentially ...

Fabrizio d'Adda di Fagagna; Philip M. Reaper; Lorena Clay-Farrace; Heike Fiegler; Philippa Carr; Thomas von Zglinicki; Gabriele Saretzki; Nigel P. Carter; Stephen P. Jackson

2003-11-05T23:59:59.000Z

476

DNA-Directed Artificial Light-Harvesting Antenna  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

DNA-Directed Artificial Light-Harvesting Antenna Authors: Dutta, P. K., Varghese, R., Nangreave, J., Lin, S., Yan, H., and Liu, Y. Title: DNA-Directed Artificial Light-Harvesting...

477

DNA Damage and Repair in Translational Oncology: An Overview  

Science Journals Connector (OSTI)

...is a molecular sensor of DNA breaks that facilitates DNA repair and controls genomic stability and apoptosis. PARP inhibitors...PARP-1 inhibitor NSC 737664 entered the NCI clinical development pipeline, we initiated a program to model pharmacodynamic responses...

Eddie Reed

2010-09-15T23:59:59.000Z

478

Single cell trapping and DNA damage analysis using microwell arrays  

E-Print Network [OSTI]

With a direct link to cancer, aging, and heritable diseases as well as a critical role in cancer treatment, the importance of DNA damage is well-established. The intense interest in DNA damage in applications ranging from ...

Wood, David

479

Improved Stool DNA Integrity Method for Early Colorectal Cancer Diagnosis  

Science Journals Connector (OSTI)

...semiautomatic extraction systems and analyzed using FL-DNA tests by CE and RT assays. The RT FL-DNA approach showed...be classified into three broad categories: stool tests [fecal occult blood test (FOBT)], endoscopic examinations (flexible sigmoidoscopy...

Claudia Rengucci; Giulia De Maio; Maura Menghi; Emanuela Scarpi; Simona Guglielmo; Pietro Fusaroli; Giancarlo Caletti; Luca Saragoni; Andrea Casadei Gardini; Wainer Zoli; Fabio Falcini; Dino Amadori; Daniele Calistri

2014-11-01T23:59:59.000Z

480

Responses of E. coli to DNA Damage and Stress  

Science Journals Connector (OSTI)

Exposure of Escherichia coli to agents that damage DNA or interfere with DNA replication results in the induction of the SOS response. A number of chromosomal genes that are repressed by the LexA protein are tran...

Toshihiro Ohta; John R. Battista…

1992-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "dna double helix" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
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481

Contributing Data to the Fleet DNA Project (Brochure), NREL ...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Photo from iStock9420677 Contributing Data to the Fleet DNA Project Sponsored by the U.S. Department of Energy, the Fleet DNA project aims to accelerate the evolution of advanced...

482

Defining functional DNA elements in the human genome  

E-Print Network [OSTI]

With the completion of the human genome sequence, attention turned to identifying and annotating its functional DNA elements. As a complement to genetic and comparative genomics approaches, the Encyclopedia of DNA Elements ...

Kellis, Manolis

483

Design of Double Salient Interior Permanent Magnet Machine Based on Mutually Coupled  

E-Print Network [OSTI]

Design of Double Salient Interior Permanent Magnet Machine Based on Mutually Coupled Reluctance--This paper presents a novel structure of double salient interior permanent magnet machine (DSIPM machine-- double salient, d-axis and q-axis inductances, cogging torque, permanent magnets, flux

Paris-Sud XI, UniversitƩ de

484

The neutrinoless double-beta decay: A test for new physics  

Science Journals Connector (OSTI)

The neutrinoless double-beta decay is not allowed in the Standard Model ... and must have a mass to allow the neutrinoless double-beta decay. Apart of one claim that the neutrinoless double-beta decay in 76Ge is ...

A. Faessler

2007-03-01T23:59:59.000Z

485

The neutrinoless double-beta decay: A test for new physics  

Science Journals Connector (OSTI)

The neutrinoless double-beta decay is not allowed in the Standard Model ... and must have a mass to allow the neutrinoless double-beta decay. Apart of one claim that the neutrinoless double-beta decay in 76Ge is ...

A. Faessler

2007-01-01T23:59:59.000Z

486

Electron spectroscopy study of single and double multiphoton ionization of strontium by visible picosecond laser light  

E-Print Network [OSTI]

795 Electron spectroscopy study of single and double multiphoton ionization of strontium by visible'ionisation multiphotonique simple et double du strontium par des impulsions picosecondes de 1011 Ơ quelque 1012 W cm-2 initial un Ʃtat excitƩ de l'ion. Abstract. 2014 Multiphoton single and double ionization of strontium

Paris-Sud XI, UniversitƩ de

487

Uncertainties in Nuclear Matrix Elements for Neutrinoless Double-Beta Decay  

E-Print Network [OSTI]

Uncertainties in Nuclear Matrix Elements for Neutrinoless Double-Beta Decay Jonathan Engel Abstract. I briefly review calculations of the matrix elements governing neutrinoless double-beta decay, in broad terms, strategies for reducing systematic error. 1. Introduction Neutrinoless double-beta (0

Engel, Jonathan

488

Alpha Backgrounds and Their Implications for Neutrinoless Double-Beta Decay Experiments Using HPGe Detectors  

E-Print Network [OSTI]

Alpha Backgrounds and Their Implications for Neutrinoless Double-Beta Decay Experiments Using HPGe and Their Implications for Neutrinoless Double-Beta Decay Experiments Using HPGe Detectors Robert A. Johnson Chair of the Supervisory Committee: Professor John F. Wilkerson Physics The observation of neutrinoless double-beta decay

Washington at Seattle, University of - Department of Physics, Electroweak Interaction Research Group

489

Double beta decay, Majorana neutrinos, and neutrino mass Frank T. Avignone III*  

E-Print Network [OSTI]

The theoretical and experimental issues relevant to neutrinoless double beta decay are reviewed. The impact more sensitive, even the nonobservation of neutrinoless double beta decay will be useful Acknowledgments 513 References 514 I. INTRODUCTION Neutrinoless double beta decay 0 is a very slow lepton

Engel, Jonathan

490

DNA-Based Optomechanical Molecular Motor  

Science Journals Connector (OSTI)

A particularly rich and promising use of this force spectroscopy setup is for constructing molecular motors. ... The resulting structure?function insights are important for future DNA motor design. ... The quantum yield of the trans to cis isomerization using 365 nm (5.5 × 10?19 J) photons is ?0.1, and hence, 10 of these photons are required per cycle. ...

Martin McCullagh; Ignacio Franco; Mark A. Ratner; George C. Schatz

2011-02-22T23:59:59.000Z

491

Machine Methods for Identifying DNA Binding Sites  

E-Print Network [OSTI]

of biological and DNA sequence information. Large numbers of genomic and proteomic projects are ongoing transcription of genetic information)? Act as a break point during genetic recombination? These are very High transformation oftranslation #12;Identification of transcription promoter motifs mRNA tRNA protein.Ć? Ć? Human: Āµ

Kon, Mark

492

PML nuclear bodies: dynamic sensors of DNA  

E-Print Network [OSTI]

-a (RAR-a).(8Ā­10) Nuclear domains containing PML are disrupted or dispersed in the lymphocytes of APLPML nuclear bodies: dynamic sensors of DNA damage and cellular stress Graham Dellaire and David P. Bazett-Jones* Summary Promyelocytic leukaemia nuclear bodies (PML NBs) are generally present in all

Dellaire, Graham

493

A DNA Nanostructure Platform for Directed Assembly of Synthetic Vaccines  

Science Journals Connector (OSTI)

A DNA Nanostructure Platform for Directed Assembly of Synthetic Vaccines ... Here we report the design and synthesis of a 1,669-nucleotide, single-stranded DNA mol. ... These DL-DNAs induced greater amts. of tumor necrosis factor-? and interleukin-6 from RAW264.7 macrophage-like cells than did a mixt. of Y-DNA with the same sequences as the corresponding DL-DNA. ...

Xiaowei Liu; Yang Xu; Tao Yu; Craig Clifford; Yan Liu; Hao Yan; Yung Chang

2012-07-03T23:59:59.000Z

494

Regulation of Gene Expression by Synthetic DNA-Binding Ligands  

E-Print Network [OSTI]

Regulation of Gene Expression by Synthetic DNA-Binding Ligands Peter B. Dervan ( ) Ā· Adam T. Poulin

Dervan, Peter B.

495

Control of DNA Strand Displacement Kinetics Using Toehold Exchange  

E-Print Network [OSTI]

static structures that self-assemble from synthetic DNA oligonucleotides of defined sequence.1-4 Fur

Zhang, David Yu

496

DNA Nanorobotics Harish Chandran, Nikhil Gopalkrishnan, and John Reif  

E-Print Network [OSTI]

of synthetic DNA to self-assemble operational molecular-scale devices. Recently there have been a series

Reif, John H.

497

String Tile Models for DNA Computing by SelfAssembly  

E-Print Network [OSTI]

Ā­based computation. These suggestions were predicated on the #12; 2 complex synthetic DNA structures invented

Winfree, Erik

498

Conformational flexibility facilitates self-assembly of complex DNA nanostructures  

E-Print Network [OSTI]

to be a superb molecular system. Synthetic DNA molecules have been programmed to assemble into a wide range

Jiang, Wen

499

A User's Guide to the Encyclopedia of DNA Elements (ENCODE)  

E-Print Network [OSTI]

The mission of the Encyclopedia of DNA Elements (ENCODE) Project is to enable the scientific and medical communities to

Bernstein, Bradley E.

500

Evolutionary theory, web-search technology combine for DNA analysis  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Sequedex: bioinformatics breakthrough Evolutionary theory, web-search technology combine for DNA analysis Sequedex: bioinformatics breakthrough with clinical & environmental...