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Sample records for discipline structural biology

  1. Structural Molecular Biology, SSRL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Highlights Training Workshops & Summer Schools Summer Students Structural Molecular Biology Illuminating Biological Structures at the Atomic and Molecular Levels Your browser...

  2. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    Biological, and Environmental Engineering, Oregon State University Williams, John M. (John M. Williams) - Petroleum Engineering program, Petroleum Institute (Abu Dhabi) Go ...

  3. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    L M N O P Q R S T U V W X Y Z Keller, Christopher (Christopher Keller) - Department of Biology, Minot State University Go back to Individual Researchers Collections: A B C D E F G ...

  4. Structural Molecular Biology, SSRL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Our Mission Our Mission The SSRL Structural Molecular Biology program operates as a integrated resource and has three primary areas (or cores) of technological research and...

  5. Report of The Structural Biology Subcommittee of The Biological and

    Office of Science (SC) Website

    Environmental Research Advisory Committee | U.S. DOE Office of Science (SC) Report of The Structural Biology Subcommittee of The Biological and Environmental Research Advisory Committee Biological and Environmental Research Advisory Committee (BERAC) BERAC Home Meetings Members Charges/Reports Current BERAC Charges Archive of BERAC Reports Charter .pdf file (135KB) BER Committees of Visitors Federal Advisory Committees BER Home Charges/Reports Report of The Structural Biology Subcommittee of

  6. The Biological Implications of the PP2A Crystal Structure

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The Biological Implications of the PP2A Crystal Structure The Biological Implications of the PP2A Crystal Structure Print Wednesday, 30 January 2008 00:00 Phosphatases, enzymes...

  7. 2010 Diffraction Methods in Structural Biology

    SciTech Connect (OSTI)

    Dr. Ana Gonzalez

    2011-03-10

    Advances in basic methodologies have played a major role in the dramatic progress in macromolecular crystallography over the past decade, both in terms of overall productivity and in the increasing complexity of the systems being successfully tackled. The 2010 Gordon Research Conference on Diffraction Methods in Structural Biology will, as in the past, focus on the most recent developments in methodology, covering all aspects of the process from crystallization to model building and refinement, complemented by examples of structural highlights and complementary methods. Extensive discussion will be encouraged and it is hoped that all attendees will participate by giving oral or poster presentations, the latter using the excellent poster display area available at Bates College. The relatively small size and informal atmosphere of the meeting provides an excellent opportunity for all participants, especially younger scientists, to meet and exchange ideas with leading methods developers.

  8. The Biological Implications of the PP2A Crystal Structure

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The Biological Implications of the PP2A Crystal Structure Print Phosphatases, enzymes that remove a phosphate group from amino-acid substrates, can be subdivided according to their...

  9. The Biological Implications of the PP2A Crystal Structure

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The Biological Implications of the PP2A Crystal Structure The Biological Implications of the PP2A Crystal Structure Print Wednesday, 30 January 2008 00:00 Phosphatases, enzymes that remove a phosphate group from amino-acid substrates, can be subdivided according to their substrate specificity. Myriad evidence has demonstrated that protein phosphatase 2A (PP2A), a family of serine/threonine-specific (Ser/Thr) phosphatases, regulates many, if not most, aspects of cellular activities and is a

  10. Administering Work Force Discipline

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2015-05-14

    The order provides requirements and responsibilities for administering work force discipline and corrective actions. Supersedes DOE O 3750.1.

  11. Administering Workforce Discipline

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2014-10-27

    To provide requirements and responsibilities for administering workforce discipline that includes disciplinary, adverse, and alternative corrective actions in the Department of Energy (DOE).

  12. The Biological Implications of the PP2A Crystal Structure

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The Biological Implications of the PP2A Crystal Structure Print Phosphatases, enzymes that remove a phosphate group from amino-acid substrates, can be subdivided according to their substrate specificity. Myriad evidence has demonstrated that protein phosphatase 2A (PP2A), a family of serine/threonine-specific (Ser/Thr) phosphatases, regulates many, if not most, aspects of cellular activities and is a critical tumor suppressor. A team at the University of Washington recently determined the first

  13. The Biological Implications of the PP2A Crystal Structure

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The Biological Implications of the PP2A Crystal Structure Print Phosphatases, enzymes that remove a phosphate group from amino-acid substrates, can be subdivided according to their substrate specificity. Myriad evidence has demonstrated that protein phosphatase 2A (PP2A), a family of serine/threonine-specific (Ser/Thr) phosphatases, regulates many, if not most, aspects of cellular activities and is a critical tumor suppressor. A team at the University of Washington recently determined the first

  14. The Biological Implications of the PP2A Crystal Structure

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The Biological Implications of the PP2A Crystal Structure Print Phosphatases, enzymes that remove a phosphate group from amino-acid substrates, can be subdivided according to their substrate specificity. Myriad evidence has demonstrated that protein phosphatase 2A (PP2A), a family of serine/threonine-specific (Ser/Thr) phosphatases, regulates many, if not most, aspects of cellular activities and is a critical tumor suppressor. A team at the University of Washington recently determined the first

  15. Electronic Structure and Excited State Dynamics in Biological and Nanoscale

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Systems | MIT-Harvard Center for Excitonics Electronic Structure and Excited State Dynamics in Biological and Nanoscale Systems February 25, 2009 at 3pm/36-428 Gregory D. Scholes Department of Chemistry, University of Toronto scholes2 abstract: After photoexcitation, energy absorbed by a molecule can be transferred efficiently over a distance of up to several tens of Ångstrom to another molecule by the process of resonance energy transfer, RET (also commonly known as electronic energy

  16. The Biological Implications of the PP2A Crystal Structure

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The Biological Implications of the PP2A Crystal Structure Print Phosphatases, enzymes that remove a phosphate group from amino-acid substrates, can be subdivided according to their substrate specificity. Myriad evidence has demonstrated that protein phosphatase 2A (PP2A), a family of serine/threonine-specific (Ser/Thr) phosphatases, regulates many, if not most, aspects of cellular activities and is a critical tumor suppressor. A team at the University of Washington recently determined the first

  17. Work Force Discipline

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    1983-03-23

    The order provides guidance and procedures and states responsibilities for maintaining work force discipline in DOE. Chg 1, dated 3-11-85; Chg 2, dated 1-6-86; Chg 3, dated 3-21-89; Chg 4, dated 8-2-90; Chg 5, dated 3-9-92; Chg 6, dated 8-21-92, cancels Chg 5.

  18. Biology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Biology @WIPP Life Begins at 250,000,000 Years WIPP's underground isn't just suited for physics experiments aiming to unlock the mysteries of the Universe, it is also a perfect...

  19. The Biological Implications of the PP2A Crystal Structure

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    susceptibility to pathogen infections. To shed light on the details of how PP2A works, Cho and Xu have determined the first crystal structure of the enzyme. Their structure...

  20. Procedure for developing biological input for the design, location, or modification of water-intake structures

    SciTech Connect (OSTI)

    Neitzel, D.A.; McKenzie, D.H.

    1981-12-01

    To minimize adverse impact on aquatic ecosystems resulting from the operation of water intake structures, design engineers must have relevant information on the behavior, physiology and ecology of local fish and shellfish. Identification of stimulus/response relationships and the environmental factors that influence them is the first step in incorporating biological information in the design, location or modification of water intake structures. A procedure is presented in this document for providing biological input to engineers who are designing, locating or modifying a water intake structure. The authors discuss sources of stimuli at water intakes, historical approaches in assessing potential/actual impact and review biological information needed for intake design.

  1. Polarized Neutron in Structural Biology Present and Future Outlook

    SciTech Connect (OSTI)

    Zhao, Jinkui [ORNL; Robertson, Lee [ORNL; Herwig, Kenneth W [ORNL; Crabb, Don [University of Virginia

    2013-01-01

    Hydrogen has a strong polarization-dependent neutron scattering cross section. This property has been exploited in the study of soft matters, especially biological macromolecules. When a polarized neutron beam is scattered off a polarized hydrogenous sample, the otherwise large hydrogen incoherent cross section is drastically reduced while the coherent signal is significantly increased. Past experiments have demonstrated the potentials and benefits of polarized neutron scattering from soft materials. The main technical challenge of polarized neutron scattering from biological matters lies at sample polarization. Dynamic nuclear polarization is a proven yet rather sophisticated technique. Its complexity is one of the main reasons for the technique's slow adoption. The future of polarized neutron scattering in biology may rest largely in neutron protein crystallography. Polarization of protein crystals is much easier to accomplish, since protein crystals are typically rather small (<<1 mm) and only require small and easy- to-operate polarization apparatuses. In addition, the high resolution nature of neutron protein crystallography means that we will be able to study individual atoms using the polarized neutron scattering technique.

  2. BioSAXS: Ways and Means to Study Structural Flexibility of Biological...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    BioSAXS: Ways and Means to Study Structural Flexibility of Biological Macromolecules Thursday, November 17, 2011 - 10:30am SSRL Conference Room 137-322 Dr. Alexander V. Shkumatov,...

  3. BioSAXS: Ways and Means to Study Structural Flexibility of Biological

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Macromolecules | Stanford Synchrotron Radiation Lightsource BioSAXS: Ways and Means to Study Structural Flexibility of Biological Macromolecules Thursday, November 17, 2011 - 10:30am SSRL Conference Room 137-322 Dr. Alexander V. Shkumatov, Biological Small Angle Scattering Group, EMBL Hamburg Small-angle X-ray scattering (SAXS) is universal low-resolution method to study native particles in solution and analyze structural changes in response to variations of external conditions. SAXS is

  4. Biological Macromolecular Structures Data from the RCSB Protein Data Bank (RCSB PDB)

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    The Research Collaboratory for Structural Bioinformatics (RCSB) is a non-profit consortium that works to improve understanding of the function of biological systems through the study of the 3-D structure of biological macromolecules. The RCSB PDB is one of three sites serving as deposition, data processing, and distribution sites of the Protein Data Bank Archive. Each site provides its own view of the primary data, thus providing a variety of tools and resources for the global community. RCSB is also the official keeper for the PDB archive, with sole access authority to the PDB archive directory structure and contents. The RCSB PDB Information Portal for Biological Macromolecular Structures offers online tools for search and retrieval, for visualizing structures, for depositing, validating, or downloading data, news and highlights, a discussion forum, and links to other areas of related research. The PDB archive is a repository of atomic coordinates and other information describing proteins and other important biological macromolecules. Structural biologists use methods such as X-ray crystallography, NMR spectroscopy, and cryo-electron microscopy to determine the location of each atom relative to each other in the molecule. They then deposit this information, which is then annotated and publicly released into the archive by the wwPDB. Results can be viewed as 3-D images or models.

  5. DOE EPSCoR Initiative in Structural and computational Biology/Bioinformatics

    SciTech Connect (OSTI)

    Wallace, Susan S.

    2008-02-21

    The overall goal of the DOE EPSCoR Initiative in Structural and Computational Biology was to enhance the competiveness of Vermont research in these scientific areas. To develop self-sustaining infrastructure, we increased the critical mass of faculty, developed shared resources that made junior researchers more competitive for federal research grants, implemented programs to train graduate and undergraduate students who participated in these research areas and provided seed money for research projects. During the time period funded by this DOE initiative: (1) four new faculty were recruited to the University of Vermont using DOE resources, three in Computational Biology and one in Structural Biology; (2) technical support was provided for the Computational and Structural Biology facilities; (3) twenty-two graduate students were directly funded by fellowships; (4) fifteen undergraduate students were supported during the summer; and (5) twenty-eight pilot projects were supported. Taken together these dollars resulted in a plethora of published papers, many in high profile journals in the fields and directly impacted competitive extramural funding based on structural or computational biology resulting in 49 million dollars awarded in grants (Appendix I), a 600% return on investment by DOE, the State and University.

  6. WHOLE CELL TOMOGRAPHY/MOLECULAR BIOLOGY/STRUCTURAL BIOLOGY: Affordable x-ray microscopy with nanoscale resolution

    SciTech Connect (OSTI)

    Evans, James E.; Blackborow, Paul; Horne, Stephen J.; Gelb, Jeff

    2013-03-01

    Biological research spans 10 orders of magnitude from angstroms to meters. While electron microscopy can reveal structural details at most of these spatial length scales, transmission electron tomography only reliably reconstructs three-dimensional (3-D) volumes of cellular material with a spatial resolution between 1-5 nm from samples less than 500 nm thick1. Most biological cells are 2-30 times thicker than this threshold, which means that a cell must be cut into consecutive slices with each slice reconstructed individually in order to approximate the contextual information of the entire cell. Fortunately, due to a larger penetration depth2, X-ray computed tomography bypasses the need to physically section a cell and enables imaging of intact cells and tissues on the micrometer or larger scale with tens to hundreds of nanometer spatial resolution. While the technique of soft x-ray microscopy has been extensively developed in synchrotron facilities, advancements in laboratory x-ray source designs now increase its accessibility by supporting commercial systems suitable for a standard laboratory. In this paper, we highlight a new commercial compact cryogenic soft x-ray microscope designed for a standard laboratory setting and explore its capabilities for mesoscopic investigations of intact prokaryotic and eukaryotic cells.

  7. National Laboratory] Basic Biological Sciences(59) Biological...

    Office of Scientific and Technical Information (OSTI)

    Achievements of structural genomics Terwilliger, Thomas C. Los Alamos National Laboratory Basic Biological Sciences(59) Biological Science Biological Science Abstract Not...

  8. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    ... for Artificial Intelligence Chinese Ceramic Society Chinese Chemical Society (CCS) Chinese Chemical Society (Taiwan) Chinese Environmental Mutagen Society (CEMS) Chinese ...

  9. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    University of Science and Technology Guzina, Bojan (Bojan Guzina) - Department of Civil Engineering, University of Minnesota Go back to Individual Researchers Collections: A B ...

  10. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    J K L M N O P Q R S T U V W X Y Z Joseph, Tim Grain (Tim Grain Joseph) - Department of Civil and Environmental Engineering, University of Alberta Go back to Individual Researchers ...

  11. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    A B C D E F G H I J K L M N O P Q R S T U V W X Y Z Colorado School of Mines, Department of Geophysics, Center for Rock Abuse Colorado School of Mines. Department of Geophysics, ...

  12. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    Sciences, Indiana University Schramm, Laurier L. (Laurier L. Schramm) - Chemistry ... Go back to Individual Researchers Collections: A B C D E F G H I J K L M N O P Q R S T U V ...

  13. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    ... (Arash Afraz) - McGovern Institute for Brain Research, Massachusetts Institute of Technology Afton, Alan D. (Alan D. Afton) - Louisiana Cooperative Fish and Wildlife Research Unit. ...

  14. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    Q R S T U V W X Y Z Pennington, Wayne D.(Wayne D.Pennington).- Department of Geological Engineering and Sciences, Michigan Technological University Perloff, Jeffrey M. (Jeffrey M. ...

  15. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    F G H I J K L M N O P Q R S T U V W X Y Z Ferguson, Robert J. (Robert J. Ferguson) - Department of Geoscience, University of Calgary Firoozabadi, Abbas (Abbas Firoozabadi) - ...

  16. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    X Y Z Zevenhoven, Ron (Ron Zevenhoven) - Heat Engineering Laboratory, bo Akademi University, Go back to Individual Researchers Collections: A B C D E F G H I J K L M N O P Q R S ...

  17. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    I J K L M N O P Q R S T U V W X Y Z Ilic, Marija D. (Marija D. Ilic) - Department of Electrical and Computer Engineering, Carnegie Mellon University Go back to Individual ...

  18. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    M N O P Q R S T U V W X Y Z Li, Kewen (Kewen Li) - Geothermal Program, Department of Petroleum Engineering, Stanford University Lin, C.-Y. Cynthia (C.-Y. Cynthia Lin) - Departments ...

  19. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    N O P Q R S T U V W X Y Z Mallinson, Richard (Richard Mallinson) - School of Chemical Engineering and Materials Science, University of Oklahoma Mohaghegh, Shahab (Shahab Mohaghegh) ...

  20. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    V W X Y Z Ursin, Bjrn (Bjrn Ursin) - Department of Petroleum Engineering and Applied Geophysics, Norwegian University of Science and Technology Go back to Individual ...

  1. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    U V W X Y Z Texas at Austin, University of (University of Texas at Austin) - Bureau of Economic Geology, Exploration Geophysics Laboratory Torres-Verdn, Carlos (Carlos ...

  2. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    W X Y Z Valk, Peter (Peter Valk) - Department of Petroleum Engineering, Texas A&M University Go back to Individual Researchers Collections: A B C D E F G H I J K L M N O P Q R ...

  3. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    E F G H I J K L M N O P Q R S T U V W X Y Z Engelder, Terry (Terry Engelder) - Department of Geosciences, Pennsylvania State University Go back to Individual Researchers ...

  4. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    H I J K L M N O P Q R S T U V W X Y Z Haszeldine, Stuart (Stuart Haszeldine) - School of GeoSciences, University of Edinburgh Hubbard, Stephen Michial (Stephen Michial Hubbard) - ...

  5. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    Fossil Fuels Go to Research Groups Preprints Provided by Individual Scientists: A B C D E F G H I J K L M N O P Q R S T U V W X Y Z Abu-Khamsin, Sidqi (Sidqi Abu-Khamsin) - ...

  6. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    S T U V W X Y Z Reynolds, Albert C. (Albert C. Reynolds) - Department of Petroleum Engineering, University of Tulsa Go back to Individual Researchers Collections: A B C D E F G H I ...

  7. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    E F G H I J K L M N O P Q R S T U V W X Y Z Dashtgard, Shahin (Shahin Dashtgard) - Department of Earth Sciences, Simon Fraser University Datta-Gupta, Akhil (Akhil Datta-Gupta) - ...

  8. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    Buckley, Jill S. (Jill S. Buckley) - Petroleum Recovery Research Center, New Mexico Tech Go back to Individual Researchers Collections: A B C D E F G H I J K L M N O P Q R S T ...

  9. Cross-Linking and Mass Spectrometry Methodologies to Facilitate Structural Biology: Finding a Path through the Maze

    SciTech Connect (OSTI)

    Merkley, Eric D.; Cort, John R.; Adkins, Joshua N.

    2013-09-01

    Multiprotein complexes, rather than individual proteins, make up a large part of the biological macromolecular machinery of a cell. Understanding the structure and organization of these complexes is critical to understanding cellular function. Chemical cross-linking coupled with mass spectrometry is emerging as a complementary technique to traditional structural biology methods and can provide low-resolution structural information for a multitude of purposes, such as distance constraints in computational modeling of protein complexes. In this review, we discuss the experimental considerations for successful application of chemical cross-linking-mass spectrometry in biological studies and highlight three examples of such studies from the recent literature. These examples (as well as many others) illustrate the utility of a chemical cross-linking-mass spectrometry approach in facilitating structural analysis of large and challenging complexes.

  10. Browse by Discipline -- E-print Network Subject Pathways: Geosciences...

    Office of Scientific and Technical Information (OSTI)

    C. Rau) - Laboratory of Physical and Structural Biology, National Institute of Child Health and Human Development Rauchfuss, Thomas B. (Thomas B. Rauchfuss) - Department of ...

  11. Theoretical Biology and Biophysics

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    6 Theoretical Biology and Biophysics Modeling biological systems and analysis and informatics of molecular and cellular biological data Mathematical Biology/Immunology Fundamental science and modeling enabling host-pathogen science Computational Structural Biology Understanding biomolecule function by determining their 3-D macromolecular structure Bioinformatics and Epidemiology Sequence analysis using advanced algorithms, software, computational hardware Contacts Group Leader Nick Hengartner

  12. NEW - DOE O 333.1, Administering Work Force Discipline

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    The order provides requirements and responsibilities for administering work force discipline and corrective actions.

  13. 7th Annual Systems Biology Symposium: Systems Biology and Engineering

    SciTech Connect (OSTI)

    Galitski, Timothy P.

    2008-04-01

    Systems biology recognizes the complex multi-scale organization of biological systems, from molecules to ecosystems. The International Symposium on Systems Biology has been hosted by the Institute for Systems Biology in Seattle, Washington, since 2002. The annual two-day event gathers the most influential researchers transforming biology into an integrative discipline investingating complex systems. Engineering and application of new technology is a central element of systems biology. Genome-scale, or very small-scale, biological questions drive the enigneering of new technologies, which enable new modes of experimentation and computational analysis, leading to new biological insights and questions. Concepts and analytical methods in engineering are now finding direct applications in biology. Therefore, the 2008 Symposium, funded in partnership with the Department of Energy, featured global leaders in "Systems Biology and Engineering."

  14. How Clean is Safe? Improving the Effectiveness of Decontamination of Structures and People Following Chemical and Biological Incidents

    SciTech Connect (OSTI)

    Vogt , B.M.

    2003-04-03

    This report describes a U.S. Department of Energy, (DOE) Chemical and Biological National Security Program project that sought to establish what is known about decontamination of structures, objects, and people following an exposure to chemical or biological materials. Specifically we sought to identify the procedures and protocols used to determine when and how people or buildings are considered ''clean'' following decontamination. To fulfill this objective, the study systematically examined reported decontamination experiences to determine what procedures and protocols are currently employed for decontamination, the timeframe involved to initiate and complete the decontamination process, how the contaminants were identified, the factors determining when people were (or were not) decontaminated, the problems encountered during the decontamination process, how response efforts of agencies were coordinated, and the perceived social psychological effects on people who were decontaminated or who participated in the decontamination process. Findings and recommendations from the study are intended to aid decision-making and to improve the basis for determining appropriate decontamination protocols for recovery planners and policy makers for responding to chemical and biological events.

  15. The Protein Data Bank in Europe (PDBe): bringing structure to biology

    SciTech Connect (OSTI)

    Velankar, Sameer; Kleywegt, Gerard J.

    2011-04-01

    Some future challenges for the PDB and its guardians are discussed and current and future activities in structural bioinformatics at the Protein Data Bank in Europe (PDBe) are described. The Protein Data Bank in Europe (PDBe) is the European partner in the Worldwide PDB and as such handles depositions of X-ray, NMR and EM data and structure models. PDBe also provides advanced bioinformatics services based on data from the PDB and related resources. Some of the challenges facing the PDB and its guardians are discussed, as well as some of the areas on which PDBe activities will focus in the future (advanced services, ligands, integration, validation and experimental data). Finally, some recent developments at PDBe are described.

  16. FINAL REPORT: DOE CONTRACT NUMBER FG0205ER64026 Biological Neutron Scattering: A Collaboration with the Oak Ridge Center for Structural Molecular Biology

    SciTech Connect (OSTI)

    Jill Trewhella

    2011-01-12

    The overarching goal of this project was to promote applications of small-angle scattering in structural molecular biology by providing model examples of cutting edge applications that demonstrate the unique capabilities and potential of the DOE national user facilities at Oak Ridge, especially the newly commissioned BioSANS. The approach taken was three-fold: (1) to engage in high impact collaborative research projects that would benefit from small-angle neutron scattering to both demonstrate the power of the technique while expanding the potential user community; (2) to provide access to scattering facilities established at the University of Utah to as broad a set of researchers as possible to increase the expertise in small-angle scattering generally; and (3) to develop new methods and tools for small-angle scattering. To these ends, three major research collaborations were pursued that resulted in a significant body of published work where neutron scattering and contrast variation played a major role. These major collaborations involved studies of protein complexes involved in (1) bacterial transcription regulation and adaptive response (a DOE/BER priority area); (2) regulation of cardiac muscle; and (3) neuronal disorders. In addition, to broaden the impact of the project, smaller collaborative efforts were supported that used either small-angle X-ray or neutron scattering. Finally, the DOE supported facilities at the University of Utah were made available to researchers on a service basis and a number of independent groups took advantage of this opportunity. In all of this work, there was an emphasis on the training of students and post docs in scattering techniques, and a set of publications (a book chapter, a review, and an encyclopedia article) were produced to guide the non-specialist potential user of scattering techniques in successful applications of the techniques. We also developed a suite of user friendly web-based computational tools currently being accessed world-wide by researchers as an aid in neutron scattering data interpretation. In all, these collaborative projects and resulted in 29 original refereed journal articles published between 2005 and 2010 and engaged groups from at least 14 Universities (10 US, 4 international) and 3 National Laboratories (2 US, 1 international). An important final initiative from this project was to begin a process for international community agreement on a set of standards for the publication of biomolecular small-angle scattering data. This initiative is being championed with the International Union of Crystallography and has engaged a number of Journal Editors and is a very important step in the maturing of this now burgeoning field.

  17. Crystallography Without Crystals: Determining the Structure of Individual Biological Molecules and Nanoparticles

    ScienceCinema (OSTI)

    Ourmazd, Abbas [University of Wisconsin, Milwaukee, Wisconsin, USA

    2010-01-08

    Ever shattered a valuable vase into 10 to the 6th power pieces and tried to reassemble it under a light providing a mean photon count of 10 minus 2 per detector pixel with shot noise? If you can do that, you can do single-molecule crystallography. This talk will outline how this can be done in principle. In more technical terms, the talk will describe how the combination of scattering physics and Bayesian algorithms can be used to reconstruct the 3-D diffracted intensity distribution from a collection of individual 2-D diffiraction patterns down to a mean photon count of 10 minus 2 per pixel, the signal level anticipated from the Linac Coherent Light Source, and hence determine the structure of individual macromolecules and nanoparticles.

  18. Biologically Inspired Synthesis Route to Three-Dimensionally Structured Inorganic Thin Films

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Schwenzer, Birgit; Morse, Daniel E.

    2008-01-01

    Inorganic thin films (hydroxide, oxide, and phosphate materials) that are textured on a submicron scale have been prepared from aqueous metal salt solutions at room temperature using vapor-diffusion catalysis. This generic synthesis approach mimics the essential advantages of the catalytic and structure-directing mechanisms observed for the formation of silica skeletons of marine sponges. Chemical composition, crystallinity, and the three-dimensional morphology of films prepared by this method are extremely sensitive to changes in the synthesis conditions, such as concentrations, reaction times, and the presence and nature of substrate materials. Focusing on different materials systems, the reaction mechanism for the formation ofmore » these thin films and the influence of different reaction parameters on the product are explained.« less

  19. Structural studies of human Naked2: A biologically active intrinsically unstructured protein

    SciTech Connect (OSTI)

    Hu Tianhui; Krezel, Andrzej M.; Li Cunxi; Coffey, Robert J. . E-mail: robert.coffey@vanderbilt.edu

    2006-12-01

    Naked1 and 2 are two mammalian orthologs of Naked Cuticle, a canonical Wnt signaling antagonist in Drosophila. Naked2, but not Naked1, interacts with transforming growth factor-{alpha} (TGF{alpha}) and escorts TGF{alpha}-containing vesicles to the basolateral membrane of polarized epithelial cells. Full-length Naked2 is poorly soluble. Since most functional domains, including the Dishevelled binding region, EF-hand, vesicle recognition, and membrane targeting motifs, reside in the N-terminal half of the protein, we expressed and purified the first 217 residues of human Naked2 and performed a functional analysis of this fragment. Its circular dichroism (CD) and nuclear magnetic resonance (NMR) spectra showed no evidence of secondary and/or tertiary structure. The fragment did not bind calcium or zinc. These results indicate that the N-terminal half of Naked2 behaves as an intrinsically unstructured protein.

  20. A common-view disciplined oscillator

    SciTech Connect (OSTI)

    Lombardi, Michael A.; Dahlen, Aaron P.

    2010-05-15

    This paper describes a common-view disciplined oscillator (CVDO) that locks to a reference time scale through the use of common-view global positioning system (GPS) satellite measurements. The CVDO employs a proportional-integral-derivative controller that obtains near real-time common-view GPS measurements from the internet and provides steering corrections to a local oscillator. A CVDO can be locked to any time scale that makes real-time common-view data available and can serve as a high-accuracy, self-calibrating frequency and time standard. Measurement results are presented where a CVDO is locked to UTC(NIST), the coordinated universal time scale maintained at the National Institute of Standards and Technology in Boulder, Colorado.

  1. DRAFT - DOE O 333.1, Administering Work Force Discipline

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    To provide requirements and responsibilities for administering workforce discipline that includes disciplinary, adverse, and alternative corrective actions in the Department of Energy (DOE).

  2. Structural Molecular Biology, SSRL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of Crystallography 2014 (IYCr2014) commemorates not only the centennial of X-ray diffraction, which allowed the detailed study of crystalline material, but also the 400th...

  3. Structural Molecular Biology, SSRL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    cellular sacs full of digestive enzymes that break down bacteria, viruses and worn-out cell parts for recycling. When this recycling process goes awry, it can cause rare metabolic...

  4. Browse by Discipline -- E-print Network Subject Pathways: Plasma...

    Office of Scientific and Technical Information (OSTI)

    of Biological and Ecological Engineering, Oregon State University Semprini, Lewis (Lewis Semprini) - School of Chemical, Biological, and Environmental Engineering, Oregon ...

  5. Browse by Discipline -- E-print Network Subject Pathways: Power...

    Office of Scientific and Technical Information (OSTI)

    of Organismic and Evolutionary Biology, Harvard University Hjek, Tom (Tom ... of Organismic and Evolutionary Biology, Harvard University Hartley, Stephen (Stephen ...

  6. Browse by Discipline -- E-print Network Subject Pathways: Fossil...

    Office of Scientific and Technical Information (OSTI)

    (Thomas D. Schneider) - Laboratory of Experimental and Computational Biology, National Cancer Institute Scott, Matthew (Matthew Scott) - Departments of Developmental Biology, ...

  7. Division of Biological and Medical Research research summary 1984-1985

    SciTech Connect (OSTI)

    Barr, S.H.

    1985-08-01

    The Division of Biological and Medical Research at Argonne National Laboratory conducts multidisciplinary research aimed at defining the biological and medical hazards to man from energy technologies and new energy options. These technically oriented studies have a strong base in fundamental research in a variety of scientific disciplines, including molecular and cellular biology, biophysics, genetics, radiobiology, pharmacology, biochemistry, chemistry, environmental toxicology, and epidemiology. This research summary is organized into six parts. The first five parts reflect the Divisional structure and contain the scientific program chapters, which summarize the activities of the individual groups during the calendar year 1984 and the first half of 1985. To provide better continuity and perspective, previous work is sometimes briefly described. Although the summaries are short, efforts have been made to indicate the range of research activities for each group.

  8. Physics Meets Biology (LBNL Summer Lecture Series)

    ScienceCinema (OSTI)

    Chu, Steve [Director, LBNL

    2011-04-28

    Summer Lecture Series 2006: If scientists could take advantage of the awesomely complex and beautiful functioning of biologys natural molecular machines, their potential for application in many disciplines would be incalculable. Nobel Laureate and Director of the Lawrence Berkeley National Laboratory Steve Chu explores Possible solutions to global warming and its consequences.

  9. Browse by Discipline -- E-print Network Subject Pathways: Power...

    Office of Scientific and Technical Information (OSTI)

    Department of Biology, University of New Mexico Nelson, Cara (Cara Nelson) - College of ... Nishiguchi) - Department of Biology, New Mexico State University Nishikawa, Kiisa (Kiisa ...

  10. Browse by Discipline -- E-print Network Subject Pathways: --...

    Office of Scientific and Technical Information (OSTI)

    ... Department of Biology, University of New Mexico Newsome, William (William Newsome) - ... Nishiguchi) - Department of Biology, New Mexico State University Nishikawa, Kiisa (Kiisa ...

  11. Browse by Discipline -- E-print Network Subject Pathways: Energy...

    Office of Scientific and Technical Information (OSTI)

    ... Andrea (Andrea Marini) - Dipartimento di Fisica, Universit di Roma Tor Vergata ... Marko) - Departments of Biochemistry, Molecular Biology, and Cell Biology & Physics and ...

  12. Browse by Discipline -- E-print Network Subject Pathways: Geosciences...

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    Xie, Xiaoliang Sunney (Xiaoliang Sunney Xie) - Department of Chemistry and Chemical Biology, Harvard University Xing, Bengang (Bengang Xing) - Division of Chemistry and Biological ...

  13. Browse by Discipline -- E-print Network Subject Pathways: Fossil...

    Office of Scientific and Technical Information (OSTI)

    Xie, Xiaoliang Sunney (Xiaoliang Sunney Xie) - Department of Chemistry and Chemical Biology, Harvard University Xing, Bengang (Bengang Xing) - Division of Chemistry and Biological ...

  14. Browse by Discipline -- E-print Network Subject Pathways: --...

    Office of Scientific and Technical Information (OSTI)

    ... Gaudet, Rachelle (Rachelle Gaudet) - Department of Molecular and Cellular Biology, Harvard ... of Organismic and Evolutionary Biology, Harvard University Giri, Ranjit K. (Ranjit K. ...

  15. Browse by Discipline -- E-print Network Subject Pathways: Power...

    Office of Scientific and Technical Information (OSTI)

    ... Vitek, Christopher (Christopher Vitek) - Department of Biology, University of Texas - Pan America Vitkup, Dennis (Dennis Vitkup) - Center for Computational Biology and ...

  16. Browse by Discipline -- E-print Network Subject Pathways: Power...

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    ... C. Thompson) - Department of Wildlife, Fish, and Conservation Biology, University of ... (Brian Todd) - Department of Wildlife, Fish, and Conservation Biology, University of ...

  17. Browse by Discipline -- E-print Network Subject Pathways: --...

    Office of Scientific and Technical Information (OSTI)

    ... C. Thompson) - Department of Wildlife, Fish, and Conservation Biology, University of ... (Brian Todd) - Department of Wildlife, Fish, and Conservation Biology, University of ...

  18. Browse by Discipline -- E-print Network Subject Pathways: --...

    Office of Scientific and Technical Information (OSTI)

    Wensheng (Wensheng Qin) - Department of Biology, Lakehead University Qiu, Anqi (Anqi Qiu) ... - Department of Ecology and Evolutionary Biology, University of Michigan Qu, Jianan ...

  19. Genomics and Systems Biology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Genomics and Systems Biology Genomics and Systems Biology Los Alamos scientists perform research in functional genomics and structural genomics, and applications for such work cover diverse fields such as energy, agriculture, and environmental cleanup. Contact Us Babetta Marrone Biofuels Program Manager Email Cheryl Kuske DOE BER Biological System Science Division Program Manager Email Kirsten McCabe Emerging Threats Program Manager Email Rebecca McDonald Bioscience Communications Email "We

  20. Penn State Multi-Discipline Tribology Group and Energy Institute Studies.

    SciTech Connect (OSTI)

    Perez, Joseph

    2001-08-05

    This presentation is a summary of the current research activities on fuels and lubricants in the Multi-discipline Tribology group and the engine test group in the Combustion Laboratory of the Pennsylvania State University. The progress areas discussed in this summary include those found in Table 1. Table 1. RESEARCH AREAS: Diesel Engine Emission Reduction; Oxygenated Fuels; Improved Friction Fuels; Vegetable Oil Lubricants; Extended Drain Lubricants; Effect of Chemical Structure on Friction and Wear. The research is of interest either directly or indirectly to the goal of this workshop, diesel engine emissions reduction. The current projects at Penn State in the areas listed above will be discussed.

  1. Browse by Discipline -- E-print Network Subject Pathways: Geosciences...

    Office of Scientific and Technical Information (OSTI)

    ... of Chemistry and Chemical Biology, Harvard University Lim, Sang-Hyun (Sang-Hyun Lim) ... of Chemistry and Chemical Biology, Harvard University Liu, Gang-yu (Gang-yu Liu) - ...

  2. Browse by Discipline -- E-print Network Subject Pathways: Fossil...

    Office of Scientific and Technical Information (OSTI)

    U V W X Y Z Ussery, David W. (David W. Ussery) - Center for Biological Sequence Analysis & Department of Systems Biology,Danmarks Tekniske Universitet Go back to Individual ...

  3. Browse by Discipline -- E-print Network Subject Pathways: --...

    Office of Scientific and Technical Information (OSTI)

    ... Department of Organismic and Evolutionary Biology, Harvard University Omiecinski, Curtis (Curtis Omiecinski) - Department of Veterinary and Biomedical Sciences, Pennsylvania State ...

  4. Browse by Discipline -- E-print Network Subject Pathways: Fossil...

    Office of Scientific and Technical Information (OSTI)

    University of Southern California Elofsson, Arne (Arne Elofsson) - Stockholm Bioinformatics Center, Stockholms Universitet Endy, Drew (Drew Endy) - Division of Biological ...

  5. Browse by Discipline -- E-print Network Subject Pathways: Geosciences...

    Office of Scientific and Technical Information (OSTI)

    C. Harrison) - Department of Biological Chemistry and Molecular Pharmacology, Harvard University Hawthorne, M. Frederick (M. Frederick Hawthorne) - Department of Chemistry ...

  6. Browse by Discipline -- E-print Network Subject Pathways: Fossil...

    Office of Scientific and Technical Information (OSTI)

    Goodrich) - Department of Neurobiology, Harvard University Gopalan, Venkat (Venkat ... - Department of Systems Biology, Harvard University Go back to Individual ...

  7. Browse by Discipline -- E-print Network Subject Pathways: Fossil...

    Office of Scientific and Technical Information (OSTI)

    and Computational Biology, National Cancer Institute Levitt, Michael (Michael ... (Xiaole Shirley Liu) - Dana-Farber Cancer Institute & Department of Biostatistics, ...

  8. Structure of trigger factor binding domain in biologically homologous complex with eubacterial ribosome reveals its chaperone action

    SciTech Connect (OSTI)

    Baram, David; Pyetan, Erez; Sittner, Assa; Auerbach-Nevo, Tamar; Bashan, Anat; Yonath, Ada (WIS-I)

    2010-07-13

    Trigger factor (TF), the first chaperone in eubacteria to encounter the emerging nascent chain, binds to the large ribosomal subunit in the vicinity of the protein exit tunnel opening and forms a sheltered folding space. Here, we present the 3.5-{angstrom} crystal structure of the physiological complex of the large ribosomal subunit from the eubacterium Deinococcus radiodurans with the N-terminal domain of TF (TFa) from the same organism. For anchoring, TFa exploits a small ribosomal surface area in the vicinity of proteins L23 and L29, by using its 'signature motif' as well as additional structural elements. The molecular details of TFa interactions reveal that L23 is essential for the association of TF with the ribosome and may serve as a channel of communication with the nascent chain progressing in the tunnel. L29 appears to induce a conformational change in TFa, which results in the exposure of TFa hydrophobic patches to the opening of the ribosomal exit tunnel, thus increasing its affinity for hydrophobic segments of the emerging nascent polypeptide. This observation implies that, in addition to creating a protected folding space for the emerging nascent chain, TF association with the ribosome prevents aggregation by providing a competing hydrophobic environment and may be critical for attaining the functional conformation necessary for chaperone activity.

  9. Biological Safety

    Broader source: Energy.gov [DOE]

    The DOE's Biological Safety Program provides a forum for the exchange of best practices, lessons learned, and guidance in the area of biological safety. This content is supported by the Biosurety Executive Team. The Biosurety Executive Team is a DOE-chartered group. The DOE Office of Worker Safety and Health Policy provides administrative support for this group. The group identifies biological safety-related issues of concern to the DOE and pursues solutions to issues identified.

  10. Browse by Discipline -- E-print Network Subject Pathways: Power...

    Office of Scientific and Technical Information (OSTI)

    ... L. Reed) - Florida Museum of Natural History Reed, Michael (Michael Reed) - Department ... Department of Biology, University of New Mexico Rudolf, Volker H. W. (Volker H. W. ...

  11. Browse by Discipline -- E-print Network Subject Pathways: Geosciences...

    Office of Scientific and Technical Information (OSTI)

    of Chemistry, Ohio State University Dyson, Jane (Jane Dyson) - Department of Molecular Biology, Scripps Research Institute Go back to Individual Researchers Collections: A B C D ...

  12. Browse by Discipline -- E-print Network Subject Pathways: Biotechnolog...

    Office of Scientific and Technical Information (OSTI)

    of South Carolina Alexandraki, Despina (Despina Alexandraki) - Institute of Molecular Biology and Biotechnology, Foundation of Research and Technology, Hellas Altschuler, Steve ...

  13. Browse by Discipline -- E-print Network Subject Pathways: Fossil...

    Office of Scientific and Technical Information (OSTI)

    of Florida Tavernarakis, Nektarios (Nektarios Tavernarakis) - Institute of Molecular Biology and Biotechnology, Foundation of Research and Technology, Hellas Tawfik, Dan S. ...

  14. Browse by Discipline -- E-print Network Subject Pathways: Fossil...

    Office of Scientific and Technical Information (OSTI)

    of South Carolina Alexandraki, Despina (Despina Alexandraki) - Institute of Molecular Biology and Biotechnology, Foundation of Research and Technology, Hellas Altschuler, Steve ...

  15. Browse by Discipline -- E-print Network Subject Pathways: Plasma...

    Office of Scientific and Technical Information (OSTI)

    Z Young, Hillary S. (Hillary S. Young) - Department of Ecology, Evolution and Marine Biology, University of California Santa Barbara Go back to Individual Researchers Collections: ...

  16. Browse by Discipline -- E-print Network Subject Pathways: Plasma...

    Office of Scientific and Technical Information (OSTI)

    W X Y Z Vokal, Barbara (Barbara Vokal) - Department of Environmental Sciences, Jozef Stefan Institute Voordouw, Gerrit (Gerrit Voordouw) - Department of Biological Sciences, ...

  17. Browse by Discipline -- E-print Network Subject Pathways: Plasma...

    Office of Scientific and Technical Information (OSTI)

    Laaksonen, Toni (Toni Laaksonen) - Department of Biology, University of Turku Lastoskie, Christian M. (Christian M. Lastoskie) - Department of Civil and Environmental Engineering, ...

  18. Browse by Discipline -- E-print Network Subject Pathways: Environmenta...

    Office of Scientific and Technical Information (OSTI)

    W X Y Z Vanlerberghe, Greg C. (Greg C. Vanlerberghe) - Department of Biological Sciences, University of Toronto at Scarborough Go back to Individual Researchers Collections: A B C...

  19. Browse by Discipline -- E-print Network Subject Pathways: Environmenta...

    Office of Scientific and Technical Information (OSTI)

    Ewen (Ewen Ritchie) - Department of Energy Technology, Aalborg University Rood, Stewart (Stewart Rood) - Department of Biological Sciences, University of Lethbridge Rosendahl, ...

  20. Browse by Discipline -- E-print Network Subject Pathways: Renewable...

    Office of Scientific and Technical Information (OSTI)

    Sullivan Sealey) - Department of Biology, University of Miami Shultis, J. Kenneth (J. Kenneth Shultis) - Department of Mechanical and Nuclear Engineering, Kansas State ...

  1. Browse by Discipline -- E-print Network Subject Pathways: Geosciences...

    Office of Scientific and Technical Information (OSTI)

    of Engineering and Applied Sciences, Harvard University Martnez, Todd J. (Todd J. ... of Chemistry and Chemical Biology, Harvard University Mller, Peter (Peter Mller) ...

  2. Browse by Discipline -- E-print Network Subject Pathways: Plasma...

    Office of Scientific and Technical Information (OSTI)

    of Engineering and Applied Sciences, Harvard University Matin, A.C. (A.C. Matin) - ... of Organismic and Evolutionary Biology, Harvard University Morel, Franois M. M. ...

  3. Browse by Discipline -- E-print Network Subject Pathways: Physics...

    Office of Scientific and Technical Information (OSTI)

    of Engineering and Applied Sciences, Harvard University Nagurka, Mark L. (Mark L. ... for Biologically Inspired Engineering, Harvard University Narain, Amitabh (Amitabh ...

  4. Browse by Discipline -- E-print Network Subject Pathways: Geosciences...

    Office of Scientific and Technical Information (OSTI)

    Jackson, Sophie (Sophie Jackson) - Department of Chemistry, University of Cambridge Jacobsen, Eric N. (Eric N. Jacobsen) - Department of Chemistry and Chemical Biology, Harvard ...

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    Office of Scientific and Technical Information (OSTI)

    York University Roder, Heinrich (Heinrich Roder) - Basic Science Division, Fox Chase Cancer Center Rosenberg, Noah (Noah Rosenberg) - Department of Biology, Stanford University. ...

  6. Browse by Discipline -- E-print Network Subject Pathways: --...

    Office of Scientific and Technical Information (OSTI)

    Biological Sciences, Virginia Tech Xiong, Yue (Yue Xiong) - Lineberger Comprehensive Cancer Center & Department of Biochemistry and Biophysics, University of North Carolina at ...

  7. Browse by Discipline -- E-print Network Subject Pathways: Power...

    Office of Scientific and Technical Information (OSTI)

    P. (Stephen P. Yanoviak) - Department of Biology, University of Arkansas at Little Rock Yeager, Kevin M. (Kevin M. Yeager) - Department of Marine Science, University of ...

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    Office of Scientific and Technical Information (OSTI)

    ... - School of Biology and Psychology, University of Newcastle upon Tyne Snell-Rood, Emilie C. (Emilie C. Snell-Rood) - Department of Ecology, Evolution, and Behavior, University of ...

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    Office of Scientific and Technical Information (OSTI)

    ... Biology, Albert Einstein College of Medicine, Yeshiva University Snell-Rood, Emilie C. (Emilie C. Snell-Rood) - Department of Ecology, Evolution, and Behavior, University of ...

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    - Department of Environmental Science, Lancaster University Nakamura, Daisuke (Daisuke ... Biology, Harvard University Nielsen, Roger L. (Roger L. Nielsen) - College of Oceanic ...

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    ... Brigham and Women's Hospital, Harvard University de ... - Department of Biology, San Francisco State University ... de Magalhes, Joo Pedro (Joo Pedro de Magalhes) ...

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    Office of Scientific and Technical Information (OSTI)

    ... Vitek, Christopher (Christopher Vitek) - Department of Biology, University of Texas - Pan America Vittoz, Pascal (Pascal Vittoz) - Dpartement d'cologie et volution, ...

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    Biology of the Chloroplast US DOE, Office of Energy Efficiency and Renewable Energy, Green Power Network Utah, University of - Energy and Geoscience Institute, GeoSpatial ...

  14. Browse by Discipline -- E-print Network Subject Pathways: Energy...

    Office of Scientific and Technical Information (OSTI)

    Y Z Xiao, John Q. (John Q. Xiao) - Department of Physics and Astronomy, University of Delaware Xing, Jianhua (Jianhua Xing) - Department of Biological Sciences, Virginia Tech Xu, ...

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    University of California at Berkeley Caro, Tim (Tim Caro) - Department of Wildlife, Fish, and Conservation Biology, University of California, Davis Caron, David (David Caron) - ...

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    Office of Scientific and Technical Information (OSTI)

    Sciences, Rutgers University Fangue, Nann A. (Nann A. Fangue) - Department of Wildlife, Fish, and Conservation Biology, University of California, Davis Farley, Kathleen A. ...

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    Idaho State University, Department of Biological Sciences, Stream Ecology Center Idaho, University of - Department of Fish and Wildlife Resources, Fish Ecology Research Laboratory ...

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    (Nann A. Fangue) - Department of Wildlife, Fish, and Conservation Biology, University of ... of Psychology, Brandeis University Fish, Frank (Frank Fish) - Department of ...

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    of Biology, Missouri State University Waits, Lisette (Lisette Waits) - Department of Fish and Wildlife Resources, University of Idaho Wakano, Joe Yuichiro (Joe Yuichiro Wakano) ...

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    ... Peter Klimley) - Department of Wildlife, Fish, and Conservation Biology, University of ... J. Kwak) - North Carolina Cooperative Fish and Wildlife Research Unit & Department of ...

  1. Browse by Discipline -- E-print Network Subject Pathways: Fossil...

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    Texas A&M University You, Lingchong (Lingchong You) - Department of Biomedical Engineering, Duke University Yu, Haiyuan (Haiyuan Yu) - Department of Biological ...

  2. Beamline 5.0.1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    1 Print Berkeley Center for Structural Biology (BCSB) Monochromatic protein crystallography Scientific discipline: Structural biology GENERAL BEAMLINE INFORMATION Operational Yes...

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    S T U V W X Y Z Sayre, Richard (Richard Sayre) - Department of Plant Cellular and Molecular Biology, Ohio State University Schiff, Eric A. (Eric A. Schiff) - Department of Physics, ...

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    H I J K L M N O P Q R S T U V W X Y Z Garca, Oscar (Oscar Garca) - Forestry Program, University of Northern British Columbia Gieg, Lisa (Lisa Gieg) - Department of Biological...

  5. Browse by Discipline -- E-print Network Subject Pathways: Plasma...

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    Marine Biology, University of Hawai'i at Manoa Horowitz, Leah S. (Leah S. Horowitz) - Social Sciences Department, Hawai'i Pacific University Hung, I-Kuai (I-Kuai Hung) - College ...

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    Y Z Xi, Weimin (Weimin Xi) - Department of Biological and Health Sciences, Texas A&M University at Kingsville Xu, Kehui "Kevin" (Kehui "Kevin" Xu) - Department of Marine Science, ...

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    Office of Scientific and Technical Information (OSTI)

    Zhang, Y.-H. Percival (Y.-H. Percival Zhang) - Department of Biological Systems Engineering, Virginia Tech Go back to Individual Researchers Collections: A B C D E F G H I J K L M ...

  8. Biological preconcentrator

    DOE Patents [OSTI]

    Manginell, Ronald P.; Bunker, Bruce C.; Huber, Dale L.

    2008-09-09

    A biological preconcentrator comprises a stimulus-responsive active film on a stimulus-producing microfabricated platform. The active film can comprise a thermally switchable polymer film that can be used to selectively absorb and desorb proteins from a protein mixture. The biological microfabricated platform can comprise a thin membrane suspended on a substrate with an integral resistive heater and/or thermoelectric cooler for thermal switching of the active polymer film disposed on the membrane. The active polymer film can comprise hydrogel-like polymers, such as poly(ethylene oxide) or poly(n-isopropylacrylamide), that are tethered to the membrane. The biological preconcentrator can be fabricated with semiconductor materials and technologies.

  9. Comparison of 20 nm silver nanoparticles synthesized with and without a gold core. Structure, dissolution in cell culture media, and biological impact on macrophages

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Munusamy, Prabhakaran; Wang, Chongmin; Engelhard, Mark H.; Baer, Donald R.; Smith, Jordan N.; Liu, Chongxuan; Kodali, Vamsi K.; Thrall, Brian D.; Chen, Shu; Porter, Alexandra E.; et al

    2015-07-15

    Widespread use of silver nanoparticles raises questions of environmental impact and toxicity. Both silver particles and silver ions formed by particle dissolution may impact biological systems. Therefore it is important to understand the characteristics of silver nanoparticles and their stability in relevant media. The synthesis route can impact physical and chemical characteristics of the particles and we report the characterization and solution stability of three types of silver nanoparticles (20 nm particles with and without gold cores and 110 nm particles with gold cores) in cell culture media with serum proteins: FBS10%/RPMI. These nanoparticles were synthesized in aqueous solution andmore » characterized using both in situ and ex situ analysis methods. Dissolution studies were carried at particle concentrations from 1 µg/ml to 50 µg/ml. Particles with gold cores had smaller crystallite size and higher apparent solubility than pure silver particles. A dissolution model was found to describe the time variation of particle size and amount of dissolved silver for particle loadings above 9 µg/ml. An effective solubility product obtained from fitting the data was higher for the 20 nm gold core particles in comparison to the pure silver or 110 nm particles. Dissolution of the nanoparticles was enhanced by presence of serum proteins contained in fetal bovine serum. In addition, the protocol of the dispersion in the medium was found to influence particle agglomeration and dissolution. Results show that particle structure can impact the concentration of dissolved silver and the dose to which cells would be exposed during in vitro studies.« less

  10. Comparison of 20 nm silver nanoparticles synthesized with and without a gold core. Structure, dissolution in cell culture media, and biological impact on macrophages

    SciTech Connect (OSTI)

    Munusamy, Prabhakaran; Wang, Chongmin; Engelhard, Mark H.; Baer, Donald R.; Smith, Jordan N.; Liu, Chongxuan; Kodali, Vamsi K.; Thrall, Brian D.; Chen, Shu; Porter, Alexandra E.; Ryan, Mary P.

    2015-07-15

    Widespread use of silver nanoparticles raises questions of environmental impact and toxicity. Both silver particles and silver ions formed by particle dissolution may impact biological systems. Therefore it is important to understand the characteristics of silver nanoparticles and their stability in relevant media. The synthesis route can impact physical and chemical characteristics of the particles and we report the characterization and solution stability of three types of silver nanoparticles (20 nm particles with and without gold cores and 110 nm particles with gold cores) in cell culture media with serum proteins: FBS10%/RPMI. These nanoparticles were synthesized in aqueous solution and characterized using both in situ and ex situ analysis methods. Dissolution studies were carried at particle concentrations from 1 µg/ml to 50 µg/ml. Particles with gold cores had smaller crystallite size and higher apparent solubility than pure silver particles. A dissolution model was found to describe the time variation of particle size and amount of dissolved silver for particle loadings above 9 µg/ml. An effective solubility product obtained from fitting the data was higher for the 20 nm gold core particles in comparison to the pure silver or 110 nm particles. Dissolution of the nanoparticles was enhanced by presence of serum proteins contained in fetal bovine serum. In addition, the protocol of the dispersion in the medium was found to influence particle agglomeration and dissolution. Results show that particle structure can impact the concentration of dissolved silver and the dose to which cells would be exposed during in vitro studies.

  11. Browse by Discipline -- E-print Network Subject Pathways: Fossil...

    Office of Scientific and Technical Information (OSTI)

    B C D E F G H I J K L M N O P Q R S T U V W X Y Z Babu, M. Madan (M. Madan Babu) - Laboratory of Molecular Biology, MRC Badger, Jonathan (Jonathan Badger) - Institute for Genomic ...

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    Office of Scientific and Technical Information (OSTI)

    R S T U V W X Y Z Qin, Wensheng (Wensheng Qin) - Department of Biology, Lakehead University Go back to Individual Researchers Collections: A B C D E F G H I J K L M N O P Q R S T U...

  13. Browse by Discipline -- E-print Network Subject Pathways: Power...

    Office of Scientific and Technical Information (OSTI)

    ... A B C D E F G H I J K L M N O P Q R S T U V W X Y Z Eawag Limnological Research Centre, Department Fish Ecology and Evolution Edinburgh, University of - School of Biological ...

  14. Browse by Discipline -- E-print Network Subject Pathways: --...

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    ... University Pereira, Henrique Miguel (Henrique Miguel Pereira) - Centro de Biologia Ambiental, Universidade de Lisboa Perera, Lalith (Lalith Perera) - Laboratory of Structural ...

  15. Browse by Discipline -- E-print Network Subject Pathways: Chemistry...

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    Johnson) - Department of Physics and Astronomy, University of Oklahoma Jonas, Ulrich (Ulrich Jonas) - Institute of Electronic Structure and Laser, Foundation for Research and ...

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    Office of Scientific and Technical Information (OSTI)

    Kafesaki, Maria (Maria Kafesaki) - Institute of Electronic Structure and Laser, Foundation ... Charles (Charles Kane) - Department of Physics and Astronomy, University of Pennsylvania ...

  17. Multi-discipline Waste Acceptance Process at the Nevada National Security Site - 13573

    SciTech Connect (OSTI)

    Carilli, Jhon T. [US Department Of Energy, Nevada Site Office, P. O. Box 98518, Las Vegas, Nevada 89193-8518 (United States)] [US Department Of Energy, Nevada Site Office, P. O. Box 98518, Las Vegas, Nevada 89193-8518 (United States); Krenzien, Susan K. [Navarro-Intera, LLC, P. O. Box 98952, Las Vegas, Nevada 89193-8952 (United States)] [Navarro-Intera, LLC, P. O. Box 98952, Las Vegas, Nevada 89193-8952 (United States)

    2013-07-01

    The Nevada National Security Site low-level radioactive waste disposal facility acceptance process requires multiple disciplines to ensure the protection of workers, the public, and the environment. These disciplines, which include waste acceptance, nuclear criticality, safety, permitting, operations, and performance assessment, combine into the overall waste acceptance process to assess low-level radioactive waste streams for disposal at the Area 5 Radioactive Waste Management Site. Four waste streams recently highlighted the integration of these disciplines: the Oak Ridge Radioisotope Thermoelectric Generators and Consolidated Edison Uranium Solidification Project material, West Valley Melter, and classified waste. (authors)

  18. Frontiers of NMR in Molecular Biology

    SciTech Connect (OSTI)

    1999-08-25

    NMR spectroscopy is expanding the horizons of structural biology by determining the structures and describing the dynamics of blobular proteins in aqueous solution, as well as other classes of proteins including membrane proteins and the polypeptides that form the aggregates diagnostic of prion and amyloid diseases. Significant results are also emerging on DNA and RNA oligomers and their complexes with proteins. This meeting focused attention on key structural questions emanating from molecular biology and how NMR spectroscopy can be used to answer them.

  19. Work with Biological Materials

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    cells, viruses), plant or soil samples (USDA quarantines), recombinant DNA, or blood-borne pathogen. Biological Use Authorization The great majority of biological work at...

  20. S09 Symposium KK, Structure-Property Relationships in Biomineralized and Biomimetic Composites

    SciTech Connect (OSTI)

    David Kisailus; Lara Estroff; Himadri S. Gupta; William J. Landis; Pablo D. Zavattieri

    2010-06-07

    The technical presentations and discussions at this symposium disseminated and assessed current research and defined future directions in biomaterials research, with a focus on structure-function relationships in biological and biomimetic composites. The invited and contributed talks covered a diverse range of topics from fundamental biology, physics, chemistry, and materials science to potential applications in developing areas such as light-weight composites, multifunctional and smart materials, biomedical engineering, and nanoscaled sensors. The invited speakers were chosen to create a stimulating program with a mixture of established and junior faculty, industrial and academic researchers, and American and international experts in the field. This symposium served as an excellent introduction to the area for younger scientists (graduate students and post-doctoral researchers). Direct interactions between participants also helped to promote potential future collaborations involving multiple disciplines and institutions.

  1. Report of the Structural Biology Subcommittee of the Biological...

    Office of Science (SC) Website

    ... David Kingsbury (absent from meeting) Chiron Corporation Emeryville, California Dr. Anthony Kossiakoff University of Chicago Chicago, Illinois Dr. Janet Smith Purdue University ...

  2. Biological conversion system

    DOE Patents [OSTI]

    Scott, C.D.

    A system for bioconversion of organic material comprises a primary bioreactor column wherein a biological active agent (zymomonas mobilis) converts the organic material (sugar) to a product (alcohol), a rejuvenator column wherein the biological activity of said biological active agent is enhanced, and means for circulating said biological active agent between said primary bioreactor column and said rejuvenator column.

  3. Ninth International Workshop on Plant Membrane Biology

    SciTech Connect (OSTI)

    Not Available

    1993-12-31

    This report is a compilation of abstracts from papers which were discussed at a workshop on plant membrane biology. Topics include: plasma membrane ATP-ases; plant-environment interactions, membrane receptors; signal transduction; ion channel physiology; biophysics and molecular biology; vaculor H+ pumps; sugar carriers; membrane transport; and cellular structure and function.

  4. Systems biology approach to bioremediation

    SciTech Connect (OSTI)

    Chakraborty, Romy; Wu, Cindy H.; Hazen, Terry C.

    2012-06-01

    Bioremediation has historically been approached as a ‘black box’ in terms of our fundamental understanding. Thus it succeeds and fails, seldom without a complete understanding of why. Systems biology is an integrated research approach to study complex biological systems, by investigating interactions and networks at the molecular, cellular, community, and ecosystem level. The knowledge of these interactions within individual components is fundamental to understanding the dynamics of the ecosystem under investigation. Finally, understanding and modeling functional microbial community structure and stress responses in environments at all levels have tremendous implications for our fundamental understanding of hydrobiogeochemical processes and the potential for making bioremediation breakthroughs and illuminating the ‘black box’.

  5. Plant Vascular Biology 2010

    SciTech Connect (OSTI)

    Ding, Biao

    2014-11-17

    This grant supported the Second International Conference on Plant Vascular Biology (PVB 2010) held July 24-28, 2010 on the campus of Ohio State University, Columbus, Ohio. Biao Ding (Ohio State University; OSU) and David Hannapel (Iowa State University; ISU) served as co-chairs of this conference. Biao Ding served as the local organizer. PVB is defined broadly here to include studies on the biogenesis, structure and function of transport systems in plants, under conditions of normal plant growth and development as well as of plant interactions with pathogens. The transport systems cover broadly the xylem, phloem, plasmodesmata and vascular cell membranes. The PVB concept has emerged in recent years to emphasize the integrative nature of the transport systems and approaches to investigate them.

  6. Beamline 5.0.3

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    0.3 Print Berkeley Center for Structural Biology (BCSB) Monochromatic protein crystallography Scientific discipline: Structural biology GENERAL BEAMLINE INFORMATION Operational Yes...

  7. Beamline 5.0.3

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Beamline 5.0.3 Print Berkeley Center for Structural Biology (BCSB) Monochromatic protein crystallography Scientific discipline: Structural biology GENERAL BEAMLINE INFORMATION...

  8. Electronic structure of superconductivity refined

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Electronic structure of superconductivity refined Electronic structure of superconductivity refined A team of physicists propose a new model that expands on a little understood aspect of the electronic structure in high-temperature superconductors. July 10, 2008 Los Alamos National Laboratory sits on top of a once-remote mesa in northern New Mexico with the Jemez mountains as a backdrop to research and innovation covering multi-disciplines from bioscience, sustainable energy sources, to plasma

  9. Biological tracer method

    DOE Patents [OSTI]

    Strong-Gunderson, J.M.; Palumbo, A.V.

    1998-09-15

    The present invention is a biological tracer method for characterizing the movement of a material through a medium, comprising the steps of: introducing a biological tracer comprising a microorganism having ice nucleating activity into a medium; collecting at least one sample of the medium from a point removed from the introduction point; and analyzing the sample for the presence of the biological tracer. The present invention is also a method for using a biological tracer as a label for material identification by introducing a biological tracer having ice nucleating activity into a material, collecting a sample of a portion of the labelled material and analyzing the sample for the presence of the biological tracer. 2 figs.

  10. Biological tracer method

    DOE Patents [OSTI]

    Strong-Gunderson, Janet M. (Ten Mile, TN); Palumbo, Anthony V. (Oak Ridge, TN)

    1998-01-01

    The present invention is a biological tracer method for characterizing the movement of a material through a medium, comprising the steps of: introducing a biological tracer comprising a microorganism having ice nucleating activity into a medium; collecting at least one sample of the medium from a point removed from the introduction point; and analyzing the sample for the presence of the biological tracer. The present invention is also a method for using a biological tracer as a label for material identification by introducing a biological tracer having ice nucleating activity into a material, collecting a sample of a portion of the labelled material and analyzing the sample for the presence of the biological tracer.

  11. Biological detector and method

    DOE Patents [OSTI]

    Sillerud, Laurel; Alam, Todd M; McDowell, Andrew F

    2013-02-26

    A biological detector includes a conduit for receiving a fluid containing one or more magnetic nanoparticle-labeled, biological objects to be detected and one or more permanent magnets or electromagnet for establishing a low magnetic field in which the conduit is disposed. A microcoil is disposed proximate the conduit for energization at a frequency that permits detection by NMR spectroscopy of whether the one or more magnetically-labeled biological objects is/are present in the fluid.

  12. Biological detector and method

    DOE Patents [OSTI]

    Sillerud, Laurel; Alam, Todd M.; McDowell, Andrew F.

    2015-11-24

    A biological detector includes a conduit for receiving a fluid containing one or more magnetic nanoparticle-labeled, biological objects to be detected and one or more permanent magnets or electromagnet for establishing a low magnetic field in which the conduit is disposed. A microcoil is disposed proximate the conduit for energization at a frequency that permits detection by NMR spectroscopy of whether the one or more magnetically-labeled biological objects is/are present in the fluid.

  13. Biological detector and method

    DOE Patents [OSTI]

    Sillerud, Laurel; Alam, Todd M; McDowell, Andrew F

    2014-04-15

    A biological detector includes a conduit for receiving a fluid containing one or more magnetic nanoparticle-labeled, biological objects to be detected and one or more permanent magnets or electromagnet for establishing a low magnetic field in which the conduit is disposed. A microcoil is disposed proximate the conduit for energization at a frequency that permits detection by NMR spectroscopy of whether the one or more magnetically-labeled biological objects is/are present in the fluid.

  14. Genomics and Systems Biology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Genomics and Systems Biology LANL leads the world in computational finishing of microbial genomes Read caption + In 2013, Los Alamos scientist Richard Sayre and his team genetically modified the organisms to harvest light more efficiently for maximum production. Overview of Research and Highlights Researchers at Los Alamos National Laboratory are using their renowned expertise in genomics, computation, and experimental biology as the foundation of a dynamic systems biology capability. Systems

  15. 2010 Plant Molecular Biology Gordon Research Conference

    SciTech Connect (OSTI)

    Michael Sussman

    2010-07-23

    The Plant Molecular Biology Conference has traditionally covered a breadth of exciting topics and the 2010 conference will continue in that tradition. Emerging concerns about food security have inspired a program with three main themes: (1) genomics, natural variation and breeding to understand adaptation and crop improvement, (2) hormonal cross talk, and (3) plant/microbe interactions. There are also sessions on epigenetics and proteomics/metabolomics. Thus this conference will bring together a range of disciplines, will foster the exchange of ideas and enable participants to learn of the latest developments and ideas in diverse areas of plant biology. The conference provides an excellent opportunity for individuals to discuss their research because additional speakers in each session will be selected from submitted abstracts. There will also be a poster session each day for a two-hour period prior to dinner. In particular, this conference plays a key role in enabling students and postdocs (the next generation of research leaders) to mingle with pioneers in multiple areas of plant science.

  16. Molecular Mechanism of Biological Proton Transport

    SciTech Connect (OSTI)

    Pomes, R.

    1998-09-01

    Proton transport across lipid membranes is a fundamental aspect of biological energy transduction (metabolism). This function is mediated by a Grotthuss mechanism involving proton hopping along hydrogen-bonded networks embedded in membrane-spanning proteins. Using molecular simulations, the authors have explored the structural, dynamic, and thermodynamic properties giving rise to long-range proton translocation in hydrogen-bonded networks involving water molecules, or water wires, which are emerging as ubiquitous H{sup +}-transport devices in biological systems.

  17. Micro/nanofabricated environments for synthetic biology

    SciTech Connect (OSTI)

    Collier, Pat [ORNL; Simpson, Michael L [ORNL

    2011-01-01

    A better understanding of how confinement, crowding and reduced dimensionality modulate reactivity and reaction dynamics will aid in the rational and systematic discovery of functionality in complex biological systems. Artificial micro- and nanofabricated structures have helped elucidate the effects of nanoscale spatial confinement and segregation on biological behavior, particularly when integrated with microfluidics, through precise control in both space and time of diffusible signals and binding interactions. Examples of nanostructured interfaces for synthetic biology include the development of cell-like compartments for encapsulating biochemical reactions, nanostructured environments for fundamental studies of diffusion, molecular transport and biochemical reaction kinetics, and regulation of biomolecular interactions as functions of micro- and nanofabricated topological constraints.

  18. Integrated Biological Control

    SciTech Connect (OSTI)

    JOHNSON, A.R.

    2002-09-01

    Biological control is any activity taken to prevent, limit, clean up, or remediate potential environmental, health and safety, or workplace quality impacts from plants, animals, or microorganisms. At Hanford the principal emphasis of biological control is to prevent the transport of radioactive contamination by biological vectors (plants, animals, or microorganisms), and where necessary, control and clean up resulting contamination. Other aspects of biological control at Hanford include industrial weed control (e.g.; tumbleweeds), noxious weed control (invasive, non-native plant species), and pest control (undesirable animals such as rodents and stinging insects; and microorganisms such as molds that adversely affect the quality of the workplace environment). Biological control activities may be either preventive (apriori) or in response to existing contamination spread (aposteriori). Surveillance activities, including ground, vegetation, flying insect, and other surveys, and apriori control actions, such as herbicide spraying and placing biological barriers, are important in preventing radioactive contamination spread. If surveillance discovers that biological vectors have spread radioactive contamination, aposteriori control measures, such as fixing contamination, followed by cleanup and removal of the contamination to an approved disposal location are typical response functions. In some cases remediation following the contamination cleanup and removal is necessary. Biological control activities for industrial weeds, noxious weeds and pests have similar modes of prevention and response.

  19. Integrated Biological Control

    SciTech Connect (OSTI)

    JOHNSON, A.R.

    2003-10-09

    Biological control is any activity taken to prevent, limit, clean up, or remediate potential environmental, health and safety, or workplace quality impacts from plants, animals, or microorganisms. At Hanford the principal emphasis of biological control is to prevent the transport of radioactive contamination by biological vectors (plants, animals, or microorganisms), and where necessary, control and clean up resulting contamination. Other aspects of biological control at Hanford include industrial weed control (e.g.; tumbleweeds), noxious weed control (invasive, non-native plant species), and pest control (undesirable animals such as rodents and stinging insects, and microorganisms such as molds that adversely affect the quality of the workplace environment). Biological control activities may be either preventive (a priori) or in response to existing contamination spread (a posteriori). Surveillance activities, including ground, vegetation, flying insect, and other surveys, and a priori control actions, such as herbicide spraying and placing biological barriers, are important in preventing radioactive contamination spread. If surveillance discovers that biological vectors have spread radioactive contamination, a posteriori control measures, such as fixing contamination, followed by cleanup and removal of the contamination to an approved disposal location are typical response functions. In some cases remediation following the contamination cleanup and removal is necessary. Biological control activities for industrial weeds, noxious weeds and pests have similar modes of prevention and response.

  20. Biological sample collector

    DOE Patents [OSTI]

    Murphy, Gloria A.

    2010-09-07

    A biological sample collector is adapted to a collect several biological samples in a plurality of filter wells. A biological sample collector may comprise a manifold plate for mounting a filter plate thereon, the filter plate having a plurality of filter wells therein; a hollow slider for engaging and positioning a tube that slides therethrough; and a slide case within which the hollow slider travels to allow the tube to be aligned with a selected filter well of the plurality of filter wells, wherein when the tube is aligned with the selected filter well, the tube is pushed through the hollow slider and into the selected filter well to sealingly engage the selected filter well and to allow the tube to deposit a biological sample onto a filter in the bottom of the selected filter well. The biological sample collector may be portable.

  1. Semiconductor Nanocrystals for Biological Imaging

    SciTech Connect (OSTI)

    Fu, Aihua; Gu, Weiwei; Larabell, Carolyn; Alivisatos, A. Paul

    2005-06-28

    Conventional organic fluorophores suffer from poor photo stability, narrow absorption spectra and broad emission feature. Semiconductor nanocrystals, on the other hand, are highly photo-stable with broad absorption spectra and narrow size-tunable emission spectra. Recent advances in the synthesis of these materials have resulted in bright, sensitive, extremely photo-stable and biocompatible semiconductor fluorophores. Commercial availability facilitates their application in a variety of unprecedented biological experiments, including multiplexed cellular imaging, long-term in vitro and in vivo labeling, deep tissue structure mapping and single particle investigation of dynamic cellular processes. Semiconductor nanocrystals are one of the first examples of nanotechnology enabling a new class of biomedical applications.

  2. Work with Biological Materials

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Work with Biological Materials Print Planning A complete Experiment Safety Sheet (ESS) is required before work can be done at the ALS. This ESS is either a part of the proposal process or may be completed as an independent document. In the ESS, identify each material (including all biological materials) with which you will be working. The regulatory oversight for biological work is very complicated and we need to understand the risk levels involved with the material you plan to use at the ALS,

  3. Work with Biological Materials

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Work with Biological Materials Print Planning A complete Experiment Safety Sheet (ESS) is required before work can be done at the ALS. This ESS is either a part of the proposal process or may be completed as an independent document. In the ESS, identify each material (including all biological materials) with which you will be working. The regulatory oversight for biological work is very complicated and we need to understand the risk levels involved with the material you plan to use at the ALS,

  4. Work with Biological Materials

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Work with Biological Materials Print Planning A complete Experiment Safety Sheet (ESS) is required before work can be done at the ALS. This ESS is either a part of the proposal...

  5. Biological Hydrogen Production Workshop

    Broader source: Energy.gov [DOE]

    The U.S. Department of Energy's (DOE's) National Renewable Energy Laboratory (NREL) held a Biological Hydrogen Production Workshop on September 24–25, 2013, in Golden, Colorado. The workshop...

  6. Biological and Environmental Research

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Biological and Environmental Research Biological and Environmental Research Understanding how genomic information is translated to functional capabilities, and the roles of Earth's biogeochemical systems so we can predict climate decades or centuries into the future. Get Expertise Cheryl Kuske (505) 665-4800 Email James Bossert (505) 667-3644 Email Manvendra Dubey (505) 665-3128 Email Kim Nitschke (505) 667-1186 Email Phil Jones (505) 667-6387 Email Cathy Wilson (505) 667-0202 Email Conducting

  7. Beamline 4.2.2

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    4.2.2 Print Molecular Biology Consortium Multiple-Wavelength Anomalous Diffraction (MAD) and Macromolecular Crystallography (MX) Scientific discipline: Structural biology GENERAL...

  8. Implications of structural genomics target selection strategies...

    Office of Scientific and Technical Information (OSTI)

    ... Country of Publication: United States Language: English Subject: 59 BASIC BIOLOGICAL SCIENCES; FOCUSING; IMPLEMENTATION; PROTEINS; RESIDUES; SIMULATION; TARGETS PFAM STRUCTURAL ...

  9. Biological Systems Science Division (BSSD) | U.S. DOE Office of Science

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    (SC) Biological Systems Science Division (BSSD) Biological and Environmental Research (BER) BER Home About Research Biological Systems Science Division (BSSD) Genomic Science DOE Bioenergy Research Centers Bioimaging Technology DOE Joint Genome Institute Structural Biology Radiochemistry & Imaging Instrumentation Radiobiology: Low Dose Radiation Research DOE Human Subjects Protection Program Climate and Environmental Sciences Division (CESD) Research Abstracts Searchable Archive of BER

  10. Green Biologics | Open Energy Information

    Open Energy Info (EERE)

    Biologics Jump to: navigation, search Name: Green Biologics Place: Oxfordshire, United Kingdom Sector: Biomass, Renewable Energy Product: Oxfordshire-based industrial biotech...

  11. Continuous mutual improvement of macromolecular structure models...

    Office of Scientific and Technical Information (OSTI)

    Accurate crystal structures of macromolecules are of high importance in the biological and biomedical fields. Models of crystal structures in the Protein Data Bank (PDB) are in ...

  12. Application of Dye-Ligand Affinity Chromatography to Structural...

    Office of Scientific and Technical Information (OSTI)

    Application of Dye-Ligand Affinity Chromatography to Structural Genomics Kim, Chang Y. Los Alamos National Laboratory Applied Life Science(60) Biological Science Biological...

  13. OTEC environmental biological oceanographic program

    SciTech Connect (OSTI)

    Hartwig, E.O.

    1981-07-01

    One of the major goals of the OTEC biological field measurement program is to assess the effect of OTEC operations on the environment. Prior understanding of the natural variability of the tropical oceanic plankton community is the most important method for determining changes due to operation of an OTEC plant. The spatial and temporal patterns of the plankton community in terms of absolute number, biomass and species composition have been investigated at potential OTEC sites. Considerable data exist which document the changes with depth of all three measurements. Diel fluctuations in number and species composition have been studied at one site. While horizontal and seasonal patterns of variability likely exist at all sites, they are subtle and remain somewhat unclear. Attempts are now being made to determine the overall trophic structure of the plankton community at these sites using these data, gut content analysis, and information already in the literature.

  14. Biological particle identification apparatus

    DOE Patents [OSTI]

    Salzman, Gary C.; Gregg, Charles T.; Grace, W. Kevin; Hiebert, Richard D.

    1989-01-01

    An apparatus and method for making multiparameter light scattering measurements from suspensions of biological particles is described. Fourteen of the sixteen Mueller matrix elements describing the particles under investigation can be substantially individually determined as a function of scattering angle and probing radiations wavelength, eight elements simultaneously for each of two apparatus configurations using an apparatus which incluees, in its simplest form, two polarization modulators each operating at a chosen frequency, one polarizer, a source of monochromatic electromagnetic radiation, a detector sensitive to the wavelength of radiation employed, eight phase-sensitive detectors, and appropriate electronics. A database of known biological particle suspensions can be assembled, and unknown samples can be quickly identified once measurements are performed on it according to the teachings of the subject invention, and a comparison is made with the database.

  15. BIOLOGICAL IRRADIATION FACILITY

    DOE Patents [OSTI]

    McCorkle, W.H.; Cern, H.S.

    1962-04-24

    A facility for irradiating biological specimens with neutrons is described. It includes a reactor wherein the core is off center in a reflector. A high-exposure room is located outside the reactor on the side nearest the core while a low-exposure room is located on the opposite side. Means for converting thermal neutrons to fast neutrons are movably disposed between the reactor core and the high and low-exposure rooms. (AEC)

  16. Mathematics and biology: The interface, challenges and opportunities

    SciTech Connect (OSTI)

    Levin, S.A. )

    1992-06-01

    The interface between mathematics and biology has long been a rich area of research, with mutual benefit to each supporting discipline. Traditional areas of investigation, such as population genetics, ecology, neurobiology, and 3-D reconstructions, have flourished, despite a rather meager environment for the funding of such work. In the past twenty years, the kind and scope of such interactions between mathematicians and biologists have changed dramatically, reaching out to encompass areas of both biology and mathematics that previously had not benefited. At the same time, with the closer integration of theory and experiment, and the increased reliance on high-speed computation, the costs of such research grew, though not the opportunities for funding. The perception became reinforced, both within the research community and at funding agencies, that although these interactions were expanding, they were not doing so at the rate necessary to meet the opportunities and needs. A workshop was held in Washington, DC, between April 28 and May 3, 1990 which drew together a broadly based group of researchers to synthesize conclusions from a group of working papers and extended discussions. The result is the report presented here, which we hope will provide a guide and stimulus to research in mathematical and computational biology for at least the next decade. The report identifies a number of grand challenges, representing a broad consensus among the participants.

  17. Beamline 5.0.2

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    2 Print Berkeley Center for Structural Biology (BCSB) Multiple-wavelength anomalous diffraction (MAD) and monochromatic protein crystallography Scientific discipline: Structural...

  18. Beamline 8.2.1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    1 Print Berkeley Center for Structural Biology (BCSB) Multiple-Wavelength Anomalous Diffraction (MAD) and Macromolecular Crystallography (MX) Scientific discipline: Structural...

  19. Abengoa Mojave Final Biological Opinion

    Broader source: Energy.gov [DOE]

    Biological Opinion on Mojave Solar, LLC's Mojave Solar Project, San Bernardino County, California (8-8-11-F-3)

  20. Elements in biological AMS

    SciTech Connect (OSTI)

    Vogel, J.S.; McAninch, J.; Freeman, S.

    1996-08-01

    AMS (Accelerator Mass Spectrometry) provides high detection sensitivity for isotopes whose half-lives are between 10 years and 100 million years. {sup 14}C is the most developed of such isotopes and is used in tracing natural and anthropogenic organic compounds in the Earth`s biosphere. Thirty-three elements in the main periodic table and 17 lanthanides or actinides have long lived isotopes, providing potential tracers for research in elemental biochemistry. Overlap of biologically interesting heavy elements and possible AMS tracers is discussed.

  1. Biological Applications of Synchrotron Radiation:

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Welcome to the Biological and Environmental Research Abstracts Database The U.S. Department of Energy's Office of Biological and Environmental Research (BER) conducts research in the areas of Climate and Environmental Sciences and Biological Systems Science. This database contains abstracts of research projects supported by the program. Work was performed at DOE Laboratories as well as at nearly 300 universities and other research institutions. This is a historical database that includes the

  2. Biological Safety | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Biological Select Agents Inspection Report: IG-0681, Concerns Regarding a Non-Viable (Dead) "Anthrax Sport" Research Project at the Oak Ridge National Laboratory Inspection ...

  3. Electronic Structure of LaOFeP - a Different Type of High Temperature...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    by the Department of Energy, Office of Basic Energy Sciences. The SSRL Structural Molecular Biology Program is supported by the Department of Energy, Office of Biological and...

  4. Biology and Medicine Division annual report, 1987

    SciTech Connect (OSTI)

    Not Available

    1988-04-01

    Modern biology is characterized by rapid change. The development of new tools and the results derived from their application to various biological systems require significant shifts in our concepts and the strategies that are adopted to analyze and elucidate mechanisms. In parallel with exciting new scientific developments our organizational structure and programmatic emphases have altered. These changes and developments have enabled the life sciences at LBL to be better positioned to create and respond to new opportunities. The work summarized in this annual report reflects a vital multifaceted research program that is in the vanguard of the areas represented. We are committed to justifying the confidence expressed by LBL through the new mission statement and reorganizational changes designed to give greater prominence to the life sciences.

  5. Biological warfare in the littorals. Final report

    SciTech Connect (OSTI)

    Larsen, R.W.

    1997-05-01

    Biological warfare (BW) has emerged as a significant threat to military operations and is particularly challenging at the operational level of warfare in a littoral environment. There are compelling reasons why an operational commander should be concerned about BW: global proliferation of biotechnology and biological weapons capabilities; suitability of BW for disrupting force projection across the littorals; and the vulnerability of American, allied and coalition forces to BW. The threat of facing an adversary capable and willing to use biological weapons will influence the commander`s application of the operational art across the six operational functions. Degradation of operational tempo, effects of psychological responses among the force, and stress on the organizational structure may challenge the command and control process. Operational intelligence will demand robust integration of technical analysis, intentions and warnings, meteorological information, and medical intelligence. The maneuver and movement processes will be taxed to function effectively when ports and airfields offer such lucrative BW targets. Biological weapons may dictate the location of operational fires assets as well as the make-up of the target lists. Operational logistics assumes great importance in the medical functions, decontamination processes, and troop replacement and unit reconstitution. Operational protection encompasses nearly every aspect of BW defense and will demand a balance between what is necessary and what is possible to protect. As daunting as the challenges appear, the operational-level commander has at his disposal many tools necessary to prepare for biological warfare in the littorals. Ultimately, the commander must convince his force, his allies, and his enemies that the command can fight effectively in a BW environment, on land and sea.

  6. Structural proteomics of minimal organisms: conservation ofprotein...

    Office of Scientific and Technical Information (OSTI)

    We alsocompare patterns in the conservation off olds among minimal organisms andthose ... Resource Relation: Journal Name: BMC Structural Biology; Journal Volume: 6; Journal Issue: ...

  7. Plant Molecular Biology 2008 Gordon Research Conference - July 13-18, 2008

    SciTech Connect (OSTI)

    Richard M. Amasino

    2009-08-28

    The Plant Molecular Biology Conference has traditionally covered a breadth of exciting topics and the 2008 conference will continue in that tradition. There will be sessions on metabolism; new methods to study genomes, proteomes and metabolomes; plant-microbe interactions; plant hormones; epigenetics. A new topic for the conference this year will be bioenergy. Thus this conference will bring together a range of disciplines to foster the exchange ideas and to permit the participants to learn of the latest developments and ideas in diverse areas of plant biology. The conference provides an excellent opportunity for individuals to discuss their research because additional speakers in each session will be selected from submitted abstracts. There will also be a poster session each day for a two-hour period prior to dinner.

  8. 2010 Tetrapyrroles, Chemistry & Biology of Gordon Research Conference

    SciTech Connect (OSTI)

    Angela Wilks

    2010-07-30

    The objective of the Chemistry & Biology of Tetrapyrroles Gordon Conference is to bring together researchers from diverse disciplines that otherwise would not interact. By bringing biologists, chemists, engineers and clinicians with a common interest in tetrapyrroles the conference provides a forum for cross-disciplinary ideas and collaboration. The perspective provided by biologists, chemists, and clinicians working in fields such as newly discovered defects in human porphyrin metabolism, the myriad of strategies for light harvesting in photosynthetic organisms, novel tetrapyrroles that serve as auxiliary chromophores or enzyme cofactors, synthetic strategies in the design of novel tetrapyrrole scaffolds, and tetrapyrrole based cell signaling and regulatory systems, makes this conference unique in the field. Over the years the growing evidence for the role of tetrapyrroles and their reactive intermediates in cell signaling and regulation has been of increasing importance at this conference. The 2010 conference on Chemistry & Biology of Tetrapyrroles will focus on many of these new frontiers as outlined in the preliminary program listed. Speakers will emphasize unpublished results and new findings in the field. The oral sessions will be followed by the highly interactive afternoon poster sessions. The poster sessions provide all conferees with the opportunity to present their latest research and to exchange ideas in a more informal setting. As in the past, this opportunity will continue during the nightly social gathering that takes place in the poster hall following the evening lectures. All conferees are encouraged to submit and present posters. At the conference the best poster in the areas of biology, chemistry and medicine will be selected by a panel of previous conference chairs.

  9. ionic liquids biological-ly derived from lignin and hemicellulose

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    biological-ly derived from lignin and hemicellulose - Sandia Energy Energy Search Icon Sandia Home Locations Contact Us Employee Locator Energy & Climate Secure & Sustainable Energy Future Stationary Power Energy Conversion Efficiency Solar Energy Wind Energy Water Power Supercritical CO2 Geothermal Natural Gas Safety, Security & Resilience of the Energy Infrastructure Energy Storage Nuclear Power & Engineering Grid Modernization Battery Testing Nuclear Fuel Cycle Defense Waste

  10. Experimental Station 14-2 | Stanford Synchrotron Radiation Lightsource

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    2 NA Status In Design Supported Techniques White Light Station Main Scientific Disciplines Structural Molecular Biology Beam Line Specifications Source Bending Magnet Energy Range ...

  11. Experimental Station 7-3 | Stanford Synchrotron Radiation Lightsource

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Spectroscopy Main Scientific Disciplines Biomedical Sciences Structural Molecular Biology Beam Line Specifications Source 20-pole, 2-Tesla wiggler, 0.8 mrad beam, Side station...

  12. Experimental Station 9-3 | Stanford Synchrotron Radiation Lightsource

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    spectroscopy Main Scientific Disciplines Biomedical Sciences Structural Molecular Biology Beam Line Specifications Source 16-pole, 2-Tesla Wiggler, 2 mrad acceptance, side...

  13. Experimental Station 14-1 | Stanford Synchrotron Radiation Lightsource

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Single wavelength anomalous diffraction (SAD) Main Scientific Disciplines Structural Molecular Biology Beam Line Specifications Source Bending Magnet Source Bending magnet, side...

  14. Biological Science | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... When Wellington Muchero got a part-time job in a plant molecular biology lab as a college student, there was no turning back. Learn how this quantitative geneticist advances ...

  15. Synthetic biology and crop engineering

    Broader source: Energy.gov [DOE]

    Breakout Session 2: Frontiers and Horizons Session 2-A: Synthetic Biology and the Promise of Biofuels Jonathan Burbaum, Program Director, Department of Energy, Office of Science, ARPA–E

  16. SINGLE MOLECULE APPROACHES TO BIOLOGY, 2010 GORDON RESEARCH CONFERENCE, JUNE 27-JULY 2, 2010, ITALY

    SciTech Connect (OSTI)

    Professor William Moerner

    2010-07-09

    The 2010 Gordon Conference on Single-Molecule Approaches to Biology focuses on cutting-edge research in single-molecule science. Tremendous technical developments have made it possible to detect, identify, track, and manipulate single biomolecules in an ambient environment or even in a live cell. Single-molecule approaches have changed the way many biological problems are addressed, and new knowledge derived from these approaches continues to emerge. The ability of single-molecule approaches to avoid ensemble averaging and to capture transient intermediates and heterogeneous behavior renders them particularly powerful in elucidating mechanisms of biomolecular machines: what they do, how they work individually, how they work together, and finally, how they work inside live cells. The burgeoning use of single-molecule methods to elucidate biological problems is a highly multidisciplinary pursuit, involving both force- and fluorescence-based methods, the most up-to-date advances in microscopy, innovative biological and chemical approaches, and nanotechnology tools. This conference seeks to bring together top experts in molecular and cell biology with innovators in the measurement and manipulation of single molecules, and will provide opportunities for junior scientists and graduate students to present their work in poster format and to exchange ideas with leaders in the field. A number of excellent poster presenters will be selected for short oral talks. Topics as diverse as single-molecule sequencing, DNA/RNA/protein interactions, folding machines, cellular biophysics, synthetic biology and bioengineering, force spectroscopy, new method developments, superresolution imaging in cells, and novel probes for single-molecule imaging will be on the program. Additionally, the collegial atmosphere of this Conference, with programmed discussion sessions as well as opportunities for informal gatherings in the afternoons and evenings in the beauty of the Il Ciocco site in Tuscany, provides an avenue for scientists from different disciplines to interact and brainstorm and promotes cross-disciplinary collaborations directed toward compelling biological problems.

  17. Renewable Hydrogen Production from Biological Systems

    Broader source: Energy.gov (indexed) [DOE]

    Hydrogen Production from Biological Systems Matthew Posewitz Colorado School of Mines DOE Biological Hydrogen Production Workshop September 24 th , 2013 H 2 production PSIIPSI...

  18. Autofermentative Biological Hydrogen Production by Cyanobacteria...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    PDF icon bioh2workshopdismukes.pdf More Documents & Publications 2013 Biological Hydrogen Production Workshop Summary Report Renewable Hydrogen Production from Biological ...

  19. Systems Biology in Prokaryote - Eukaryote Symbiosis | Stanford Synchrotron

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Radiation Lightsource Systems Biology in Prokaryote - Eukaryote Symbiosis Monday, June 25, 2012 - 12:00pm SLAC, SSRL Main Conference Room, 137-322 Allen M. Orville, Brookhaven National Laboratory Frontier challenges for macromolecular crystallography (MX) now include determining structures of trapped reactive intermediates, large macromolecules and viruses, membrane proteins, protein-protein complexes, and protein-nucleic acid complexes. Although structure and function are intimately linked,

  20. Thin film composition with biological substance and method of making

    SciTech Connect (OSTI)

    Campbell, A.A.; Song, L.

    1999-09-28

    The invention provides a thin-film composition comprising an underlying substrate of a first material including a plurality of attachment sites; a plurality of functional groups chemically attached to the attachment sites of the underlying substrate; and a thin film of a second material deposited onto the attachment sites of the underlying substrate, and a biologically active substance deposited with the thin-film. Preferably the functional groups are attached to a self assembling monolayer attached to the underlying substrate. Preferred functional groups attached to the underlying substrate are chosen from the group consisting of carboxylates, sulfonates, phosphates, optionally substituted, linear or cyclo, alkyl, alkene, alkyne, aryl, alkylaryl, amine, hydroxyl, thiol, silyl, phosphoryl, cyano, metallocenyl, carbonyl, and polyphosphate. Preferred materials for the underlying substrate are selected from the group consisting of a metal, a metal alloy, a plastic, a polymer, a proteic film, a membrane, a glass or a ceramic. The second material is selected from the group consisting of inorganic crystalline structures, inorganic amorphous structures, organic crystalline structures, and organic amorphous structures. Preferred second materials are phosphates, especially calcium phosphates and most particularly calcium apatite. The biologically active molecule is a protein, peptide, DNA segment, RNA segment, nucleotide, polynucleotide, nucleoside, antibiotic, antimicrobial, radioisotope, chelated radioisotope, chelated metal, metal salt, anti-inflammatory, steroid, nonsteroid anti-inflammatory, analgesic, antihistamine, receptor binding agent, or chemotherapeutic agent, or other biologically active material. Preferably the biologically active molecule is an osteogenic factor consisting of the compositions listed above.

  1. Thin film composition with biological substance and method of making

    DOE Patents [OSTI]

    Campbell, Allison A.; Song, Lin

    1999-01-01

    The invention provides a thin-film composition comprising an underlying substrate of a first material including a plurality of attachment sites; a plurality of functional groups chemically attached to the attachment sites of the underlying substrate; and a thin film of a second material deposited onto the attachment sites of the underlying substrate, and a biologically active substance deposited with the thin-film. Preferably the functional groups are attached to a self assembling monolayer attached to the underlying substrate. Preferred functional groups attached to the underlying substrate are chosen from the group consisting of carboxylates, sulfonates, phosphates, optionally substituted, linear or cyclo, alkyl, alkene, alkyne, aryl, alkylaryl, amine, hydroxyl, thiol, silyl, phosphoryl, cyano, metallocenyl, carbonyl, and polyphosphate. Preferred materials for the underlying substrate are selected from the group consisting of a metal, a metal alloy, a plastic, a polymer, a proteic film, a membrane, a glass or a ceramic. The second material is selected from the group consisting of inorganic crystalline structures, inorganic amorphus structures, organic crystalline structures, and organic amorphus structures. Preferred second materials are phosphates, especially calcium phosphates and most particularly calcium apatite. The biologically active molecule is a protein, peptide, DNA segment, RNA segment, nucleotide, polynucleotide, nucleoside, antibiotic, antimicrobal, radioisotope, chelated radioisotope, chelated metal, metal salt, anti-inflamatory, steriod, nonsteriod anti-inflammatory, analgesic, antihistamine, receptor binding agent, or chemotherapeutic agent, or other biologically active material. Preferably the biologically active molecule is an osteogenic factor the compositions listed above.

  2. Robust, High-Throughput Analysis of Protein Structures

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Robust, High-Throughput Analysis of Protein Structures Print Scientists have developed a fast and efficient way to determine the structure of proteins, shortening a process that often takes years into a matter of days. The Structurally Integrated BiologY for Life Sciences (SIBYLS) beamline at the ALS has implemented the world's highest-throughput biological-solution x-ray scattering beamline enabling genomic-scale protein-structure characterization. Coupling brilliant x rays from one of the

  3. Robust, High-Throughput Analysis of Protein Structures

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Robust, High-Throughput Analysis of Protein Structures Print Scientists have developed a fast and efficient way to determine the structure of proteins, shortening a process that often takes years into a matter of days. The Structurally Integrated BiologY for Life Sciences (SIBYLS) beamline at the ALS has implemented the world's highest-throughput biological-solution x-ray scattering beamline enabling genomic-scale protein-structure characterization. Coupling brilliant x rays from one of the

  4. Robust, High-Throughput Analysis of Protein Structures

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Robust, High-Throughput Analysis of Protein Structures Print Scientists have developed a fast and efficient way to determine the structure of proteins, shortening a process that often takes years into a matter of days. The Structurally Integrated BiologY for Life Sciences (SIBYLS) beamline at the ALS has implemented the world's highest-throughput biological-solution x-ray scattering beamline enabling genomic-scale protein-structure characterization. Coupling brilliant x rays from one of the

  5. Robust, High-Throughput Analysis of Protein Structures

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Robust, High-Throughput Analysis of Protein Structures Robust, High-Throughput Analysis of Protein Structures Print Wednesday, 28 October 2009 00:00 Scientists have developed a fast and efficient way to determine the structure of proteins, shortening a process that often takes years into a matter of days. The Structurally Integrated BiologY for Life Sciences (SIBYLS) beamline at the ALS has implemented the world's highest-throughput biological-solution x-ray scattering beamline enabling

  6. Robust, High-Throughput Analysis of Protein Structures

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Robust, High-Throughput Analysis of Protein Structures Print Scientists have developed a fast and efficient way to determine the structure of proteins, shortening a process that often takes years into a matter of days. The Structurally Integrated BiologY for Life Sciences (SIBYLS) beamline at the ALS has implemented the world's highest-throughput biological-solution x-ray scattering beamline enabling genomic-scale protein-structure characterization. Coupling brilliant x rays from one of the

  7. Robust, High-Throughput Analysis of Protein Structures

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Robust, High-Throughput Analysis of Protein Structures Print Scientists have developed a fast and efficient way to determine the structure of proteins, shortening a process that often takes years into a matter of days. The Structurally Integrated BiologY for Life Sciences (SIBYLS) beamline at the ALS has implemented the world's highest-throughput biological-solution x-ray scattering beamline enabling genomic-scale protein-structure characterization. Coupling brilliant x rays from one of the

  8. Frontiers of Plant Cell Biology: Signals and Pathways, System-Based Approaches 22nd Symposium in Plant Biology (University of California-Riverside)

    SciTech Connect (OSTI)

    Minorsky, Peter V.

    2003-06-01

    The symposium ''Frontiers of Plant Cell Biology: Signals and Pathways, Systems-Based Approaches'' was held January 15-18, 2003 at the Riverside Convention Center in Riverside, California. The host organization for the symposium was the Center for Plant Cell Biology (CEPCEB) at the University of California, Riverside (UCR). The meeting, focusing on systems-based approaches to plant cell biology research, was the first of this kind in the field of plant biology. The speakers and nearly 100 posters placed emphasis on recent developments in plant cellular biology and molecular genetics, particularly those employing emerging genomic tools, thereby sharing the most current knowledge in the field and stimulating future advances. In attendance were many well-established scientists and young investigators who approach plant cell biology from different but complementary conceptual and technical perspectives. Indeed, many disciplines are converging in the field of cell biology, producing synergies that will enable plant scientists to determine the function of gene products in the context of living cells in whole organisms. New, cross-disciplinary collaborations, as well as the involvement of computer scientists and chemists in plant biology research, are likely additional outcomes of the symposium. The program included 39 invited session speakers and workshop/panel speakers. Sessions were convened on the following themes: Cell-Cell Communication; Protein Trafficking; Cell Surface, Extracellular Matrix and Cell Wall; Signal Transduction; Signal Transduction and Proteosome; and Systems-Based Approaches to Plant Cell Biology. Workshops on Chemical Genetics and Visual Microscopy were also presented. Abstracts from each of the speaker presentations, as well as the posters presented at the meeting were published in a program booklet given to the 239 faculty members, researchers, postdoctoral scientists and graduate students in attendance. The booklet thus serves as a reference for symposium attendees to locate additional information about a topic of their particular interest and to contact other investigators. In addition, an article reviewing the symposium by science writer Peter V. Minorsky appeared in the June 2003 issue of Plant Physiology, a special issue devoted to systems-based approaches in the study of the model plant Arabidopsis (article submitted as part of this Final Technical Report).

  9. DOE Chair of Excellence in Environmental Disciplines-Final Technical Report

    SciTech Connect (OSTI)

    Kurunganty, Sastry; Loran, Roberto; Roque-Malherbe, Rolando; Hijazi, Yazan; Nieto, Santander; Gomez, Will A.; Duconge, Jose; Cotto, María del C.; Muniz, Carlos; Diaz, Francisco J.; Neira, Carlos F.; Marquez, Francisco; Del Valle, W.; Thommes, M.

    2014-02-19

    The report Massie Chair of Excellence Program at Universidad del Turabo, contract DE-FG02-95EW12610, during the period of 9/29/1995 to 9/29/2011. The initial program aims included development of academic programs in the Environmental Sciences and Engineering, and Research and Development focused initially on environmentally friendly processes and later revised also include: renewable energy and international cooperation. From 1995 -2005, the Program at UT lead the establishment of the new undergraduate program in electrical engineering at the School of Engineering (SoE), worked on requirements to achieve ABET accreditation of the SoE B.S. Mechanical Engineering and B.S. Electrical Engineering programs, mentored junior faculty, taught undergraduate courses in electrical engineering, and revised the electrical engineering curriculum. Engineering undergraduate laboratories were designed and developed. The following research sub-project was developed: Research and development of new perovskite-alumina hydrogen permeable asymmetrical nanostructured membranes for hydrogen purification, and extremely high specific surface area silica materials for hydrogen storage in the form of ammonia, Dr. Rolando Roque-Malherbe Subproject PI, Dr. Santander Nieto and Mr. Will Gómez Research Assistants. In 2006, the Massie Chair of Excellence Program was transferred to the National Nuclear Security Agency, NNSA and DNN. DoE required a revised proposal aligned with the priorities of the Administration. The revised approved program aims included: (1) Research (2) Student Development: promote the development of minority undergraduate and graduate students through research teams, internships, conferences, new courses; and, (3) Support: (a) Research administration and (b) Dissemination through international conferences, the UT Distinguished Lecturer Series in STEM fields and at the annual Universidad del Turabo (UT) Researchers Conference. Research included: Sub-Project 1: Synthesis and Characterization of low Refractive Index Aerogel Silica for Cherenkov Counters- Dr. Rolando Roque-Malherbe Sub-project PI, Dr. Jose Duconge Sub-project Co-PI, Dr. Santander Nieto Assistant Researcher, Francisco Diaz and Carlos Neira Associate Researchers. The initial aim of this sub-project was changed to the synthesis and characterization of extremely high specific surface area aerogel silica for gas storage. A high specific surface area silica gel that has applications in gas drying, cleaning operation useful in nuclear industry in process was developed. Sub-Project 2: Investigation Study of Magnetic and Electronic Transport Properties at Material Interfaces in Magnetic Multilayer Heterostructure using Gd. – Dr. Yazan Hijazi, Sub-project Co-PI. UT developed the capability and infrastructure to produce high quality thin-film magnetic films and magnetic multilayer structures with fine control over film quality and thickness using sputter deposition capability to perform in-house electric and magnetic characterization of these films. The research experimentally quantified the effect of Gd incorporation within the magnetic multilayer structure and produce magnetic media with exchanged decoupled multilevel magnetic anisotropy. From September 2006 to September 2011 the Massie Chair produced nineteen (19) publications, (including 3 books), five (5) presentations and three (3) international conferences abstracts. A total of fourteen (14) undergraduates and (6) graduate students acquired research experience. Two Ph.D. students presented their dissertations on topics related to nuclear energy and graduated as follows: María Cotto (May 2009) and Eric Calderón (May 2011). Five of the participating undergraduate students graduated: Ramon Polanco (BSME, May 2009), Jason Pérez (BSEE, May 2008), Rafael Colón (BSME, May 2008), Jessenia Marfisi (BS Chemistry, May 2008). Eleven (11) students were sent to National Laboratories (LANL, SNL and LLNL), NNSA and DoE facilities for summer internships. Twenty eight (28) undergraduate students participated in Summer Internships (2010, 2011) at the Puerto Rico Energy Center (PREC). Four international energy symposiums were held aligned with the DoE and the NNSA missions and dissemination of Massie Chair research activities (660 attendees). Academic programs developed or revised under advice of the Massie Chair: Ph.D. in Environmental Sciences (revised); MSc in Environmental Sciences (revised); MSc in Mechanical Engineering with concentration in Alternative Energy (new); BS in Industrial Management & Engineering (revised to fulfill the ABET requirements); BS in Civil Engineering including an environmental option (new); BS in Electrical Engineering (revised); and, Associate in Renewable Energy (new). The Puerto Rico Energy Center (PREC) was designed and developed under the Massie Chair initiative. Thirty-three (33) proposals were developed and submitted during the period of which 12 were approved in the amount of $ $1,931,306.

  10. California Valley Solar Ranch Biological Assessment

    Broader source: Energy.gov [DOE]

    Biological Assessment for the California Valley Solar Ranch Project San Luis Obispo County, California

  11. SC e-journals, Biology/Genetics

    Office of Scientific and Technical Information (OSTI)

    Biology/Genetics ACM Transactions on Applied Perception (TAP) ACS Chemical Biology ACS Synthetic Biology Acta Biotheoretica Acta Neuropathologica Advances in Bioinformatics - OAJ Advances in Health Sciences Education Agriculture and Human Values Agroforestry Systems American Journal of Agricultural and Biological Science - OAJ American Journal of Medical Genetics Amino Acids Analyst Analytical and Bioanalytical Chemistry Analytical Biochemistry Anatomical Record, The Anatomy and Embryology

  12. The Biological Implications of the PP2A Crystal Structure

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    groups are not removed in needed situations) is associated with multiple human cancers, Alzheimer's Disease, and increased susceptibility to pathogen infections. To shed light on...

  13. Data publication with the structural biology data grid supports...

    Office of Scientific and Technical Information (OSTI)

    J. ; Bonvin, Alexandre M.J. J. ; Borek, Dominika ; Brett, Tom J. ; Caflisch, Amedeo ; Chang, Chung-I more ; Chazin, Walter J. ; Corbett, Kevin D. ; Cosgrove, Michael S. ; ...

  14. Structural Biology | U.S. DOE Office of Science (SC)

    Office of Science (SC) Website

    Radiobiology: Low Dose Radiation Research DOE Human Subjects Protection Program Climate and Environmental Sciences Division (CESD) Research Abstracts Searchable Archive of ...

  15. Storage by Scientific Discipline

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Heat & Cool » Water Heating » Storage Water Heaters Storage Water Heaters Consider energy efficiency when selecting a conventional storage water heater to avoid paying more over its lifetime. | Photo courtesy of ©iStockphoto/JulNichols. Consider energy efficiency when selecting a conventional storage water heater to avoid paying more over its lifetime. | Photo courtesy of ©iStockphoto/JulNichols. Conventional storage water heaters remain the most popular type of water heating system

  16. 2013 METALS IN BIOLOGY GORDON RESEARCH CONFERENCE, JANUARY 20-25, 2013

    SciTech Connect (OSTI)

    Rosenzweig, Amy

    2013-01-25

    Typical topics for lectures and posters include: biochemical and biophysical characterization of new metal containing proteins, enzymes, nucleic acids, factors, and chelators from all forms of life; synthesis, detailed characterization, and reaction chemistry of biomimetic compounds; novel crystal and solution structures of biological molecules and synthetic metal-chelates; discussions of the roles that metals play in medicine, maintenance of the environment, and biogeochemical processes; metal homeostasis; application of theory and computations to the structure and mechanism of metal-containing biological systems; and novel applications of spectroscopy to metals in biological systems.

  17. Purification, crystallization and structural elucidation ofD...

    Office of Scientific and Technical Information (OSTI)

    Section F, Structural Biology Communications; Journal Volume: 72; Journal Issue: (1) ; 01, 2016 Publisher: International Union of Crystallography Research Org: Advanced Photon ...

  18. Artificial photosynthesis combines biology with technology for...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Artificial photosynthesis combines biology with technology for sustainable energy ... photosynthetic net primary production (NPP) to support our GDP and nutrition. ...

  19. Autofermentative Biological Hydrogen Production by Cyanobacteria |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Autofermentative Biological Hydrogen Production by Cyanobacteria Autofermentative Biological Hydrogen Production by Cyanobacteria Presentation by Charles Dismukes, Rutgers University, at the Biological Hydrogen Production Workshop held September 24-25, 2013, at the National Renewable Energy Laboratory in Golden, Colorado. PDF icon bio_h2_workshop_dismukes.pdf More Documents & Publications 2013 Biological Hydrogen Production Workshop Summary Report Renewable Hydrogen

  20. Rapid classification of biological components

    DOE Patents [OSTI]

    Thompson, Vicki S.; Barrett, Karen B.; Key, Diane E.

    2006-01-24

    A method is disclosed for analyzing a biological sample by antibody profiling for identifying forensic samples or for detecting the presence of an analyte. In an illustrative embodiment of the invention, the analyte is a drug, such as marijuana, cocaine, methamphetamine, methyltestosterone, or mesterolone. The method involves attaching antigens to the surface of a solid support in a preselected pattern to form an array wherein the locations of the antigens are known; contacting the array with the biological sample such that a portion of antibodies in the sample reacts with and binds to antigens in the array, thereby forming immune complexes; washing away antibodies that do form immune complexes; and detecting the immune complexes, thereby forming an antibody profile. Forensic samples are identified by comparing a sample from an unknown source with a sample from a known source. Further, an assay, such as a test for illegal drug use, can be coupled to a test for identity such that the results of the assay can be positively correlated to the subject's identity.

  1. Rapid classification of biological components

    DOE Patents [OSTI]

    Thompson, Vicki S.; Barrett, Karen B.; Key, Diane E.

    2013-10-15

    A method is disclosed for analyzing a biological sample by antibody profiling for identifying forensic samples or for detecting the presence of an analyte. In an illustrative embodiment of the invention, the analyte is a drug, such as marijuana, cocaine (crystalline tropane alkaloid), methamphetamine, methyltestosterone, or mesterolone. The method involves attaching antigens to a surface of a solid support in a preselected pattern to form an array wherein the locations of the antigens are known; contacting the array with the biological sample such that a portion of antibodies in the sample reacts with and binds to antigens in the array, thereby forming immune complexes; washing away antibodies that do not form immune complexes; and detecting the immune complexes, thereby forming an antibody profile. Forensic samples are identified by comparing a sample from an unknown source with a sample from a known source. Further, an assay, such as a test for illegal drug use, can be coupled to a test for identity such that the results of the assay can be positively correlated to a subject's identity.

  2. Rapid classification of biological components

    DOE Patents [OSTI]

    Thompson, Vicki S.; Barrett, Karen B.; Key, Diane E.

    2010-03-23

    A method is disclosed for analyzing a biological sample by antibody profiling for identifying forensic samples or for detecting the presence of an analyte. In an illustrative embodiment of the invention, the analyte is a drug, such as marijuana, Cocaine (crystalline tropane alkaloid), methamphetamine, methyltestosterone, or mesterolone. The method involves attaching antigens of the surface of a solid support in a preselected pattern to form an array wherein the locations of the antigens are known; contacting the array with the biological sample such that a portion of antibodies in the sample reacts with and binds to antigens in the array, thereby forming immune complexes; washing away antibodies that do not form immune complexes; and detecting the immune complexes, thereby forming an antibody profile. Forensic samples are identified by comparing a sample from an unknown source with a sample from a known source. Further, an assay, such as a test for illegal drug use, can be coupled to a test for identity such that the results of the assay can be positively correlated to a subject's identity.

  3. Rapid classification of biological components

    DOE Patents [OSTI]

    Thompson, Vicki S.; Barrett, Karen B.; Key, Diane E.

    2010-03-23

    A method is disclosed for analyzing a biological sample by antibody profiling for identifying forensic samples or for detecting the presence of an analyte. In an illustrative embodiment of the invention, the analyte is a drug, such as marijuana, cocaine (crystalline tropane alkaloid), methamphetamine, methyltestosterone, or mesterolone. The method involves attaching antigens to a surface of a solid support in a preselected pattern to form an array wherein the locations of the antigens are known; contacting the array with the biological sample such that a portion of antibodies in the sample reacts with and binds to antigens in the array, thereby forming immune complexes; washing away antibodies that do not form immune complexes; and detecting the immune complexes, thereby forming an antibody profile. Forensic samples are identified by comparing a sample from an unknown source with a sample from a known source. Further, an assay, such as a test for illegal drug use, can be coupled to a test for identity such that the results of the assay can be positively correlated to a subject's identity.

  4. Method of measurement in biological systems

    DOE Patents [OSTI]

    Turteltaub, K.W.; Vogel, J.S.; Felton, J.S.; Gledhill, B.L.: Davis, J.C.; Stanker, L.H.

    1993-05-11

    A method is disclosed of quantifying molecules in biological substances, comprising: selecting a biological host in which radioisotopes are present in concentrations equal to or less than those in the ambient biosphere; preparing a long-lived radioisotope labeled reactive chemical specie; administering the chemical specie to the biological host in doses sufficiently low to avoid significant overt damage to the biological system; allowing a period of time to elapse sufficient for dissemination and interaction of the chemical specie with the host throughout the biological system of the host; isolating a reacted fraction of the biological substance from the host in a manner sufficient to avoid contamination of the substance from extraneous sources; converting the fraction of biological substance by suitable means to a material which efficiently produces charged ions in at least one of several possible ion sources without introduction of significant isotopic fractionation; and measuring the radioisotope concentration in the material by means of direct isotopic counting.

  5. Invited Review Article: Advanced light microscopy for biological space research

    SciTech Connect (OSTI)

    De Vos, Winnok H.; Beghuin, Didier; Schwarz, Christian J.; Jones, David B.; Loon, Jack J. W. A. van

    2014-10-15

    As commercial space flights have become feasible and long-term extraterrestrial missions are planned, it is imperative that the impact of space travel and the space environment on human physiology be thoroughly characterized. Scrutinizing the effects of potentially detrimental factors such as ionizing radiation and microgravity at the cellular and tissue level demands adequate visualization technology. Advanced light microscopy (ALM) is the leading tool for non-destructive structural and functional investigation of static as well as dynamic biological systems. In recent years, technological developments and advances in photochemistry and genetic engineering have boosted all aspects of resolution, readout and throughput, rendering ALM ideally suited for biological space research. While various microscopy-based studies have addressed cellular response to space-related environmental stressors, biological endpoints have typically been determined only after the mission, leaving an experimental gap that is prone to bias results. An on-board, real-time microscopical monitoring device can bridge this gap. Breadboards and even fully operational microscope setups have been conceived, but they need to be rendered more compact and versatile. Most importantly, they must allow addressing the impact of gravity, or the lack thereof, on physiologically relevant biological systems in space and in ground-based simulations. In order to delineate the essential functionalities for such a system, we have reviewed the pending questions in space science, the relevant biological model systems, and the state-of-the art in ALM. Based on a rigorous trade-off, in which we recognize the relevance of multi-cellular systems and the cellular microenvironment, we propose a compact, but flexible concept for space-related cell biological research that is based on light sheet microscopy.

  6. Breaking the Biological Barriers to Cellulosic Ethanol, June...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Breaking the Biological Barriers to Cellulosic Ethanol, June 2006 Breaking the Biological Barriers to Cellulosic Ethanol, June 2006 Breaking the Biological Barriers to Cellulosic...

  7. Electrostatic thin film chemical and biological sensor

    DOE Patents [OSTI]

    Prelas, Mark A.; Ghosh, Tushar K.; Tompson, Jr., Robert V.; Viswanath, Dabir; Loyalka, Sudarshan K.

    2010-01-19

    A chemical and biological agent sensor includes an electrostatic thin film supported by a substrate. The film includes an electrostatic charged surface to attract predetermined biological and chemical agents of interest. A charge collector associated with said electrostatic thin film collects charge associated with surface defects in the electrostatic film induced by the predetermined biological and chemical agents of interest. A preferred sensing system includes a charge based deep level transient spectroscopy system to read out charges from the film and match responses to data sets regarding the agents of interest. A method for sensing biological and chemical agents includes providing a thin sensing film having a predetermined electrostatic charge. The film is exposed to an environment suspected of containing the biological and chemical agents. Quantum surface effects on the film are measured. Biological and/or chemical agents can be detected, identified and quantified based on the measured quantum surface effects.

  8. Biological & Environmental Research Abstracts Database

    Office of Scientific and Technical Information (OSTI)

    Welcome to the Biological and Environmental Research Abstracts Database The U.S. Department of Energy's Office of Biological and Environmental Research (BER) conducts research in the areas of Climate and Environmental Sciences and Biological Systems Science. This database contains abstracts of research projects supported by the program. Work was performed at DOE Laboratories as well as at nearly 300 universities and other research institutions. This is a historical database that includes the

  9. Method for photo-altering a biological system to improve biological effect

    DOE Patents [OSTI]

    Hill, Richard A. (Irvine, CA); Doiron, Daniel R. (Santa Ynez, CA); Crean, David H. (Santa Barbara, CA)

    2000-08-01

    Photodynamic therapy is a new adjunctive therapy for filtration surgery that does not use chemotherapy agents or radiation, but uses pharmacologically-active sensitizing compounds to produce a titratable, localized, transient, post operative avascular conjunctiva. A photosensitizing agent in a biological system is selectively activated by delivering the photosensitive agent to the biological system and laser activating only a spatially selected portion of the delivered photosensitive agent. The activated portion of the photosensitive agent reacts with the biological system to obtain a predetermined biological effect. As a result, an improved spatial disposition and effectuation of the biological effect by the photosensitive agent in the biological system is achieved.

  10. A brief history of the Biology Complex

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Complex Most of the "Biology" buildings were constructed in early 1945 and were built as expansion capability for the uranium preparation operations being conducted across the...

  11. Molecular biology of signal transduction in plants

    SciTech Connect (OSTI)

    Not Available

    1991-01-01

    This volume contains abstracts of oral presentations and poster sessions of the 1991 Cold Springs Harbor Meeting entitled Molecular Biology of Signal Transduction in Plants.

  12. Biological and Environmental Research Advisory Committee (BERAC...

    Office of Science (SC) Website

    Sharlene Weatherwax .pdf file (768KB) Office of Biological and Environmental Research Update Gary Geernaert .pdf file (5.8MB) Climate and Environmental Sciences Division Update ...

  13. Developing Biological Specifications for Fish Friendly Turbines

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Biological Specifications for Fish Friendly Turbines The U.S. Department of Energy's ... which environmen- tal attributes, such as entrainment survival for fish, are emphasized. ...

  14. Developing Biological Specifications for Fish Friendly Turbines

    Broader source: Energy.gov [DOE]

    This factsheet explains studies conducted in a highly reproducible manner to examine the biological effects to fish exposed to a shear environment in the laboratory.

  15. 2013 Biological Hydrogen Production Workshop Summary Report ...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    November 2013 summary report for the 2013 Biological Hydrogen Production Workshop. PDF icon bioh2workshopfinalreport.pdf More Documents & Publications The Hydrogen Program at ...

  16. Repurposing the translation apparatus for synthetic biology ...

    Office of Scientific and Technical Information (OSTI)

    Journal Name: Current Opinion in Chemical Biology Additional Journal Information: Journal ... Publisher: Elsevier Sponsoring Org: USDOE Advanced Research Projects Agency - Energy ...

  17. Novel nuclear magnetic resonance techniques for studying biological molecules

    SciTech Connect (OSTI)

    Laws, David D.

    2000-06-01

    Over the fifty-five year history of Nuclear Magnetic Resonance (NMR), considerable progress has been made in the development of techniques for studying the structure, function, and dynamics of biological molecules. The majority of this research has involved the development of multi-dimensional NMR experiments for studying molecules in solution, although in recent years a number of groups have begun to explore NMR methods for studying biological systems in the solid-state. Despite this new effort, a need still exists for the development of techniques that improve sensitivity, maximize information, and take advantage of all the NMR interactions available in biological molecules. In this dissertation, a variety of novel NMR techniques for studying biomolecules are discussed. A method for determining backbone ({phi}/{psi}) dihedral angles by comparing experimentally determined {sup 13}C{sub a}, chemical-shift anisotropies with theoretical calculations is presented, along with a brief description of the theory behind chemical-shift computation in proteins and peptides. The utility of the Spin-Polarization Induced Nuclear Overhauser Effect (SPINOE) to selectively enhance NMR signals in solution is examined in a variety of systems, as are methods for extracting structural information from cross-relaxation rates that can be measured in SPINOE experiments. Techniques for the production of supercritical and liquid laser-polarized xenon are discussed, as well as the prospects for using optically pumped xenon as a polarizing solvent. In addition, a detailed study of the structure of PrP 89-143 is presented. PrP 89-143 is a 54 residue fragment of the prion proteins which, upon mutation and aggregation, can induce prion diseases in transgenic mice. Whereas the structure of the wild-type PrP 89-143 is a generally unstructured mixture of {alpha}-helical and {beta}-sheet conformers in the solid state, the aggregates formed from the PrP 89-143 mutants appear to be mostly {beta}-sheet.

  18. Biology Division. Progress report, August 1, 1982-September 30, 1983

    SciTech Connect (OSTI)

    Not Available

    1984-01-01

    The Biology Division is the component of the Oak Ridge National Laboratory that investigates the potential adverse health effects of energy-related substances. The body of this report provides summaries of the aims, scope and progress of the research of groups of investigators in the Division during the period of August 1, 1982, through September 30, 1983. At the end of each summary is a list of publications covering the same period (published or accepted for publication). For convenience, the summaries are assembled under Sections in accordance with the current organizational structure of the Biology Division; each Section begins with an overview. It will be apparent, however, that currents run throughout the Division and that the various programs support and interact with each other.

  19. Structural Genomics: Expectations and Reality

    Office of Scientific and Technical Information (OSTI)

    Impact of Structural Genomics: Expectations and Outcomes Running head: Same Authors: John-Marc Chandonia 1 and Steven E. Brenner 1,2 Address for correspondence: Steven E. Brenner Department of Plant and Microbial Biology 461A Koshland Hall University of California Berkeley, CA 94720-3102 email: brenner@compbio.berkeley.edu fax: (413) 280-7813 Affiliations: 1 - Berkeley Structural Genomics Center, Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA 2 -

  20. Sharpening Our Tools: Algal Biology Toolbox Workshop

    Broader source: Energy.gov [DOE]

    The Bioenergy Technologies Office (BETO) will host the Algal Biology Toolbox Workshop on May 24-25, 2016, in San Diego, California. Because biological productivity is a key driver for the economic viability of algae-based biofuels, improving on the performance of native strains is a critical element of the research efforts funded by the Advanced Algal Systems program at BETO.

  1. A national facility for biological cryo-electron microscopy

    SciTech Connect (OSTI)

    Saibil, Helen R.; Grnewald, Kay; Stuart, David I.

    2015-01-01

    This review provides a brief update on the use of cryo-electron microscopy for integrated structural biology, along with an overview of the plans for the UK national facility for electron microscopy being built at the Diamond synchrotron. Three-dimensional electron microscopy is an enormously powerful tool for structural biologists. It is now able to provide an understanding of the molecular machinery of cells, disease processes and the actions of pathogenic organisms from atomic detail through to the cellular context. However, cutting-edge research in this field requires very substantial resources for equipment, infrastructure and expertise. Here, a brief overview is provided of the plans for a UK national three-dimensional electron-microscopy facility for integrated structural biology to enable internationally leading research on the machinery of life. State-of-the-art equipment operated with expert support will be provided, optimized for both atomic-level single-particle analysis of purified macromolecules and complexes and for tomography of cell sections. The access to and organization of the facility will be modelled on the highly successful macromolecular crystallography (MX) synchrotron beamlines, and will be embedded at the Diamond Light Source, facilitating the development of user-friendly workflows providing near-real-time experimental feedback.

  2. Understanding of solar wind structure might be wrong

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Solar wind structure misunderstood Understanding of solar wind structure might be wrong The plasma particles flowing from the Sun and blasting past the Earth might be configured more as a network of tubes than a river-like stream. September 7, 2010 Los Alamos National Laboratory sits on top of a once-remote mesa in northern New Mexico with the Jemez mountains as a backdrop to research and innovation covering multi-disciplines from bioscience, sustainable energy sources, to plasma physics and new

  3. Biological and chemical technologies research. FY 1995 annual summary report

    SciTech Connect (OSTI)

    1996-03-01

    The annual summary report presents the fiscal year (FY) 1995 research activities and accomplishments for the United States Department of Energy (DOE) Biological and Chemical Technologies Research (BCTR) Program. This BCTR program resides within the Office of Industrial Technologies (OIT) of the Office of Energy Efficiency and Renewable Energy (EE). The annual summary report for 1995 (ASR 95) contains the following: program description (including BCTR program mission statement, historical background, relevance, goals and objectives); program structure and organization, selected technical and programmatic highlights for 1995; detailed descriptions of individual projects; a listing of program output, including a bibliography of published work; patents; and awards arising from work supported by the BCTR.

  4. Method of measurement in biological systems

    DOE Patents [OSTI]

    Turteltaub, K.W.; Vogel, J.S.; Felton, J.S.; Gledhill, B.L.; Davis, J.C.

    1994-12-27

    Disclosed is a method of quantifying molecules in biological substances comprising: a. selecting a biological host in which radioisotopes are present in concentrations equal to or less than those in the ambient biosphere, b. preparing a long-lived radioisotope labeled reactive chemical specie, c. administering the chemical specie to the biological host in doses sufficiently low to avoid significant overt damage to the biological system, d. allowing a period of time to elapse sufficient for dissemination and interaction of the chemical specie with the host throughout the biological system of the host, e. isolating a reacted fraction of the biological substance from the host in a manner sufficient to avoid contamination of the substance from extraneous sources, f. converting the fraction of biological substance by suitable means to a material which efficiently produces charged ions in at least one of several possible ion sources without introduction of significant isotopic fractionation, and, g. measuring the radioisotope concentration in the material by means of direct isotopic counting. 5 figures.

  5. Method of measurement in biological systems

    DOE Patents [OSTI]

    Turteltaub, Kenneth W.; Vogel, John S.; Felton, James S.; Gledhill, Barton L.; Davis, Jay C.; Stanker, Larry H.

    1993-05-11

    Disclosed is a method of quantifying molecules in biological substances, comprising: a. selecting a biological host in which radioisotopes are present in concentrations equal to or less than those in the ambient biosphere, b. preparing a long-lived radioisotope labeled reactive chemical specie, c. administering said chemical specie to said biological host in doses sufficiently low to avoid significant overt damage to the biological system thereof, d. allowing a period of time to elapse sufficient for dissemination and interaction of said chemical specie with said host throughout said biological system of said host, e. isolating a reacted fraction of the biological substance from said host in a manner sufficient to avoid contamination of said substance from extraneous sources, f. converting said fraction of biological substance by suitable means to a material which efficiently produces charged ions in at least one of several possible ion sources without introduction of significant isotopic fractionation, and, g. measuring the radioisotope concentration in said material by means of direct isotopic counting.

  6. Method of measurement in biological systems

    DOE Patents [OSTI]

    Turteltaub, Kenneth W.; Vogel, John S.; Felton, James S.; Gledhill, Barton L.; Davis, Jay C.

    1994-01-01

    Disclosed is a method of quantifying molecules in biological substances comprising: a. selecting a biological host in which radioisotopes are present in concentrations equal to or less than those in the ambient biosphere, b. preparing a long-lived radioisotope labeled reactive chemical specie, c. administering said chemical specie to said biological host in doses sufficiently low to avoid significant overt damage to the biological system thereof, d. allowing a period of time to elapse sufficient for dissemination and interaction of said chemical specie with said host throughout said biological system of said host, e. isolating a reacted fraction of the biological substance from said host in a manner sufficient to avoid contamination of said substance from extraneous sources, f. converting said fraction of biological substance by suitable means to a material which efficiently produces charged ions in at least one of several possible ion sources without introduction of significant isotopic fractionation, and, g. measuring the radioisotope concentration in said material by means of direct isotopic counting.

  7. Grand Challenges for Biological and Environmental Research: A Long-Term Vision

    SciTech Connect (OSTI)

    Arkin, A.; Baliga, N.; Braam, J.; Church, G.; Collins, J; Cottingham, R.; Ecker, J.; Gerstein, M.; Gilna, P.; Greenberg, J.; Handelsman, J.; Hubbard, S.; Joachimiak, A.; Liao, J.; Looger, L.; Meyerowitz, E.; Mjolness, E.; Petsko, G.; Sayler, G.; Simpson, M.; Stacey, G.; Sussman, M.; Tiedje, J.; Bader, D.; Cessi, P.; Collins, W.; Denning, S.; Dickinson, R.; Easterling, D.; Edmonds, J.; Feddema, J.; Field, C.; Fridlind, A.; Fung, I.; Held, I.; Jackson, R.; Janetos, A.; Large, W.; Leinen, M.; Leung, R.; Long, S.; Mace, G.; Masiello, C.; Meehl, G.; Ort, D.; Otto-Bliesner, B.; Penner, J.; Prather, M.; Randall, D.; Rasch, P.; Schneider, E.; Shugart, H.; Thornton, P.; Washington, W.; Wildung, R.; Wiscombe, W.; Zak, D.; Zhang, M.; Bielicki, J.; Buford, M.; Cleland, E.; Dale, V.; Duke, C.; Ehleringer, J.; Hecht, A.; Kammen, D.; Marland, G.; Pataki, D.; Riley, M. Robertson, P.; Hubbard, S.

    2010-12-01

    The interactions and feedbacks among plants, animals, microbes, humans, and the environment ultimately form the world in which we live. This world is now facing challenges from a growing and increasingly affluent human population whose numbers and lifestyles are driving ever greater energy demand and impacting climate. These and other contributing factors will make energy and climate sustainability extremely difficult to achieve over the 20-year time horizon that is the focus of this report. Despite these severe challenges, there is optimism that deeper understanding of our environment will enable us to mitigate detrimental effects, while also harnessing biological and climate systems to ensure a sustainable energy future. This effort is advanced by scientific inquiries in the fields of atmospheric chemistry and physics, biology, ecology, and subsurface science - all made possible by computing. The Office of Biological and Environmental Research (BER) within the Department of Energy's (DOE) Office of Science has a long history of bringing together researchers from different disciplines to address critical national needs in determining the biological and environmental impacts of energy production and use, characterizing the interplay of climate and energy, and collaborating with other agencies and DOE programs to improve the world's most powerful climate models. BER science focuses on three distinct areas: (1) What are the roles of Earth system components (atmosphere, land, oceans, sea ice, and the biosphere) in determining climate? (2) How is the information stored in a genome translated into microbial, plant, and ecosystem processes that influence biofuel production, climate feedbacks, and the natural cycling of carbon? (3) What are the biological, geochemical, and physical forces that govern the behavior of Earth's subsurface environment? Ultimately, the goal of BER science is to support experimentation and modeling that can reliably predict the outcomes and behaviors of complex biological and environmental systems, leading to robust solutions for DOE missions and strategic goals. In March 2010, the Biological and Environmental Research Advisory Committee held the Grand Challenges for Biological and Environmental Research: A Long-Term Vision workshop to identify scientific opportunities and grand challenges for BER science in the coming decades and to develop an overall strategy for drafting a long-term vision for BER. Key workshop goals included: (1) Identifying the greatest scientific challenges in biology, climate, and the environment that DOE will face over a 20-year time horizon. (2) Describing how BER should be positioned to address those challenges. (3) Determining the new and innovative tools needed to advance BER science. (4) Suggesting how the workforce of the future should be trained in integrative system science. This report lays out grand research challenges for BER - in biological systems, climate, energy sustainability, computing, and education and workforce training - that can put society on a path to achieve the scientific evidence and predictive understanding needed to inform decision making and planning to address future energy needs, climate change, water availability, and land use.

  8. STRUCtural Simulator

    Energy Science and Technology Software Center (OSTI)

    2004-07-01

    STRUC-ANL is a derivative of the FLUSTR-ANL finite element code. It contains only the structural capabilities of the original fluid-structural FLUSTR code.

  9. 2013 Biological Hydrogen Production Workshop Summary Report

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    and the implications for both in vivo and in vitro activity were discussed. "Self-repair" is often cited as an advantage of biological systems, and some of the mechanisms...

  10. Biological denitrification of high concentration nitrate waste

    DOE Patents [OSTI]

    Francis, Chester W.; Brinkley, Frank S.

    1977-01-01

    Biological denitrification of nitrate solutions at concentrations of greater than one kilogram nitrate per cubic meter is accomplished anaerobically in an upflow column having as a packing material a support for denitrifying bacteria.

  11. Algal Biology Program at Los Alamos

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Algal Biology Program at Los Alamos gets a star October 11, 2011 LOS ALAMOS, New Mexico, October 11, 2011-Richard Sayre, one of the nation's top specialists in algae and...

  12. Renewable Hydrogen Production from Biological Systems

    Broader source: Energy.gov [DOE]

    Presentation by Matthew Posewitz, Colorado School of Mines, at the Biological Hydrogen Production Workshop held September 24-25, 2013, at the National Renewable Energy Laboratory in Golden, Colorado.

  13. Genetics and molecular biology of breast cancer

    SciTech Connect (OSTI)

    King, M.C.; Lippman, M.

    1992-12-31

    This volume contains the abstracts of oral presentations and poster sessions presented at the Cold Springs Harbor Meeting on Cancer Cells, this meeting entitled Genetics and Molecular Biology of Breast Cancer.

  14. Biological Air Emissions Control | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Biological Air Emissions Control Biological Air Emissions Control Innovative Technology Enables Low-Cost, Energy-Efficient Treatment of Industrial Exhaust Streams Air quality standards are becoming more stringent for the U.S. wood products industry. Emissions of volatile organic compounds (VOCs) and hazardous air pollutants (HAPs) (including methanol, formaldehyde, acetylaldehyde, and acrolein) during production of wood products must be tightly controlled. Conventional VOCs and HAPs emission

  15. BCTR: Biological and Chemical Technologies Research 1994 annual summary report

    SciTech Connect (OSTI)

    Petersen, G.

    1995-02-01

    The annual summary report presents the fiscal year (FY) 1994 research activities and accomplishments for the United States Department of Energy (DOE) Biological and Chemical Technologies Research (BCTR) Program of the Advanced Industrial Concepts Division (AICD). This AICD program resides within the Office of Industrial Technologies (OIT) of the Office of Energy Efficiency and Renewable Energy (EE). Although the OIT was reorganized in 1991 and AICD no longer exists, this document reports on efforts conducted under the former structure. The annual summary report for 1994 (ASR 94) contains the following: program description (including BCTR program mission statement, historical background, relevance, goals and objectives); program structure and organization, selected technical and programmatic highlights for 1994; detailed descriptions of individual projects; a listing of program output, including a bibliography of published work; patents, and awards arising from work supported by BCTR.

  16. Structure of the human MLH1 N-terminus: implications for predispositio...

    Office of Scientific and Technical Information (OSTI)

    Article Resource Relation: Journal Name: Acta crystallographica. Section F, Structural biology communications; Journal Volume: 71; Journal Issue: Pt 8; Other Information: PMCID:...

  17. Full-length G[alpha subscript q]–phospholipase C-[beta]3 structure...

    Office of Scientific and Technical Information (OSTI)

    Resource Type: Journal Article Resource Relation: Journal Name: Nature Structural & Molecular Biology; Journal Volume: 20; Journal Issue: (3) ; 03, 2013 Publisher: Nature ...

  18. Structural analysis of flexible proteins in solution by SmallAngle...

    Office of Scientific and Technical Information (OSTI)

    Resource Relation: Journal Name: Journal of Structural Biology; Journal Volume: 158; Related Information: Journal Publication Date: 2007 Research Org: Ernest Orlando Lawrence ...

  19. Structural analysis of flexible proteins in solution by SmallAngle...

    Office of Scientific and Technical Information (OSTI)

    ... Resource Relation: Journal Name: Journal of Structural Biology; Journal Volume: 158; Related Information: Journal Publication Date: 2007 Research Org: Ernest Orlando Lawrence ...

  20. Full-length Gαq-phospholipase C-β3 structure reveals interfaces...

    Office of Scientific and Technical Information (OSTI)

    Resource Type: Journal Article Resource Relation: Journal Name: Nature Structural & Molecular Biology; Journal Volume: 20; Journal Issue: (3) ; 03, 2013 Publisher: Nature ...

  1. Synthetic Biology for Advanced Fuels (Opening Keynote Address...

    Office of Scientific and Technical Information (OSTI)

    Synthetic Biology for Advanced Fuels (Opening Keynote Address - 2010 JGI User Meeting) Citation Details In-Document Search Title: Synthetic Biology for Advanced Fuels (Opening ...

  2. Flow cytometry aids basic cell biology research and drug discovery

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Flow cytometry aids basic cell biology research and drug discovery Flow cytometry aids basic cell biology research and drug discovery Life Technologies Corporation and LANL have ...

  3. Solar Energy Education. Renewable energy activities for biology...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    biology Citation Details In-Document Search Title: Solar Energy Education. Renewable energy activities for biology You are accessing a document from the Department of Energy's ...

  4. Techno-Economic Boundary Analysis of Biological Pathways to Hydrogen...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Techno-Economic Boundary Analysis of Biological Pathways to Hydrogen Production (2009) Techno-Economic Boundary Analysis of Biological Pathways to Hydrogen Production (2009) ...

  5. Copy of Synthetic Biology of Novel Thermophilic Bacteria for...

    Office of Scientific and Technical Information (OSTI)

    Copy of Synthetic Biology of Novel Thermophilic Bacteria for Enhanced Production of ... Citation Details In-Document Search Title: Copy of Synthetic Biology of Novel Thermophilic ...

  6. Biological and Environmental Research (BER) Homepage | U.S. DOE...

    Office of Science (SC) Website

    DISCOVERING the physical, chemical, and biological drivers of climate change. Understanding Complex Biological and Environmental Systems across many spatial and temporal scales by ...

  7. Nanojunction Sensors for the Detection of chemical and Biological...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Nanojunction Sensors for the Detection of chemical and Biological Species DOE Grant ... gaps for various applications, including chemical and biological sensors, magnetoresistive ...

  8. Technoeconomic Boundary Analysis of Biological Pathways to Hydrogen...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Technoeconomic Boundary Analysis of Biological Pathways to Hydrogen Production Technoeconomic Boundary Analysis of Biological Pathways to Hydrogen Production Report documenting the ...

  9. UK Biotechnology and Biological Sciences Research Council | Open...

    Open Energy Info (EERE)

    Biotechnology and Biological Sciences Research Council Jump to: navigation, search Name: UK Biotechnology and Biological Sciences Research Council Place: London, United Kingdom...

  10. Detter, John C. [Los Alamos National Laboratory] Basic Biological

    Office of Scientific and Technical Information (OSTI)

    State of the Art for Autonomous Detection Systems using Genomic Sequencing Detter, John C. Los Alamos National Laboratory Basic Biological Sciences(59) Biological Science...

  11. Biological Conversion of Sugars to Hydrocarbons Technology Pathway...

    Office of Scientific and Technical Information (OSTI)

    Biological Conversion of Sugars to Hydrocarbons Technology Pathway Citation Details In-Document Search Title: Biological Conversion of Sugars to Hydrocarbons Technology Pathway ...

  12. California: Breakthrough in Algae Biology | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Breakthrough in Algae Biology California: Breakthrough in Algae Biology January 31, 2014 - 12:00am Addthis Researchers at the Scripps Institution of Oceanography at University of ...

  13. Genetics and Molecular Biology of Hydrogen Metabolism in Sulfate...

    Office of Scientific and Technical Information (OSTI)

    Genetics and Molecular Biology of Hydrogen Metabolism in Sulfate-Reducing Bacteria Citation Details In-Document Search Title: Genetics and Molecular Biology of Hydrogen Metabolism ...

  14. Biology and Medicine Division annual report, 1981-1982. [Lead abstract

    SciTech Connect (OSTI)

    Not Available

    1983-04-01

    Separate abstracts were prepared for the 61 research reports in the 1981-1982 annual report for the Biology and Medicine Division of the Lawrence Berkeley Laboratory. Programs reviewed include research medicine, Donner Pavilion, environmental physiology, radiation biophysics and structural biophysics. (KRM)

  15. Department of Energy's Biological and Environmental Research Strategic Data Roadmap for Earth System Science

    SciTech Connect (OSTI)

    Williams, Dean N.; Palanisamy, Giri; Shipman, Galen; Boden, Thomas A.; Voyles, Jimmy W.

    2014-04-25

    Rapid advances in experimental, sensor, and computational technologies and techniques are driving exponential growth in the volume, acquisition rate, variety, and complexity of scientific data. This wealth of scientifically meaningful data has tremendous potential to lead to scientific discovery. However, to achieve scientific breakthroughs, these data must be exploitable—they must be analyzed effectively and efficiently and the results shared and communicated easily within the wider Department of Energy’s (DOE’s) Biological and Environmental Research (BER) Climate and Environmental Sciences Division (CESD) community. The explosion in data complexity and scale makes these tasks exceedingly difficult to achieve, particularly given that an increasing number of disciplines are working across techniques, integrating simulation and experimental or observational results (see Table 5 in Appendix 2). Consequently, we need new approaches to data management, analysis, and visualization that provide research teams with easy-to-use and scalable end-to-end solutions. These solutions must facilitate (and where feasible, automate and capture) every stage in the data lifecycle (shown in Figure 1), from collection to management, annotation, sharing, discovery, analysis, and visualization. In addition, the core functionalities are the same across climate science communities, but they require customization to adapt to specific needs and fit into research and analysis workflows. To this end, the mission of CESD’s Data and Informatics Program is to integrate all existing and future distributed CESD data holdings into a seamless and unified environment for the acceleration of Earth system science.

  16. Financing Structures

    Broader source: Energy.gov [DOE]

    Various structures can be used to finance a clean energy project or develop a clean energy financing program that will support many projects. Find more information on the financing structures...

  17. The Intersection of Physics and Biology

    ScienceCinema (OSTI)

    Liphardt, Jan [University of California, Berkeley, California, United States

    2010-09-01

    In April 1953, Watson and Crick largely defined the program of 20th century biology: obtaining the blueprint of life encoded in the DNA. Fifty years later, in 2003, the sequencing of the human genome was completed. Like any major scientific breakthrough, the sequencing of the human genome raised many more questions than it answered. I'll brief you on some of the big open problems in cell and developmental biology, and I'll explain why approaches, tools, and ideas from the physical sciences are currently reshaping biological research. Super-resolution light microscopies are revealing the intricate spatial organization of cells, single-molecule methods show how molecular machines function, and new probes are clarifying the role of mechanical forces in cell and tissue function. At the same time, Physics stands to gain beautiful new problems in soft condensed matter, quantum mechanics, and non-equilibrium thermodynamics.

  18. The acquisition of dangerous biological materials :

    SciTech Connect (OSTI)

    Aceto, Donato Gonzalo; Astuto-Gribble, Lisa M.; Gaudioso, Jennifer M.

    2007-11-01

    Numerous terrorist organizations have openly expressed interest in producing and deploying biological weapons. However, a limiting factor for many terrorists has been the acquisition of dangerous biological agents, as evidenced by the very few successful instances of biological weapons use compared to the number of documented hoaxes. Biological agents vary greatly in their ability to cause loss of life and economic damage. Some agents, if released properly, can kill many people and cause an extensive number of secondary infections; other agents will sicken only a small number of people for a short period of time. Consequently, several biological agents can potentially be used to perpetrate a bioterrorism attack but few are likely capable of causing a high consequence event. It is crucial, from a US national security perspective, to more deeply understand the likelihood that terrorist organizations can acquire the range of these agents. Few studies have attempted to comprehensively compile the technical information directly relevant to the acquisition of dangerous bacteria, viruses and toxins. In this report, technical fact sheets were assembled for 46 potentially dangerous biological agents. Much of the information was taken from various research sources which could ultimately and significantly expedite and improve bioterrorism threat assessments. By systematically examining a number of specific agent characteristics included in these fact sheets, it may be possible to detect, target, and implement measures to thwart future terrorist acquisition attempts. In addition, the information in these fact sheets may be used as a tool to help laboratories gain a rudimentary understanding of how attractive a method laboratory theft is relative to other potential acquisition modes.

  19. Biological cell classification by multiangle light scattering

    DOE Patents [OSTI]

    Salzman, G.C.; Crowell, J.M.; Mullaney, P.F.

    1975-06-03

    The specification is directed to an apparatus and method for detecting light scattering from a biological cell. Light, preferably from a coherent source of radiation, intercepts an individual biological cell in a stream of cells passing through the beam. Light scattered from the cell is detected at a selected number of angles between 0 and 90/sup 0/ to the longitudinal axis of the beam with a circular array of light responsive elements which produce signals representative of the intensity of light incident thereon. Signals from the elements are processed to determine the light-scattering pattern of the cell and therefrom its identity.

  20. Biology and Medicine Division: Annual report 1986

    SciTech Connect (OSTI)

    Not Available

    1987-04-01

    The Biology and Medicine Division continues to make important contributions in scientific areas in which it has a long-established leadership role. For 50 years the Division has pioneered in the application of radioisotopes and charged particles to biology and medicine. There is a growing emphasis on cellular and molecular applications in the work of all the Division's research groups. The powerful tools of genetic engineering, the use of recombinant products, the analytical application of DNA probes, and the use of restriction fragment length polymorphic DNA are described and proposed for increasing use in the future.

  1. Spectroscopic diagnostics for bacteria in biologic sample

    DOE Patents [OSTI]

    El-Sayed, Mostafa A.; El-Sayed, Ivan H.

    2002-01-01

    A method to analyze and diagnose specific bacteria in a biologic sample using spectroscopy is disclosed. The method includes obtaining the spectra of a biologic sample of a non-infected patient for use as a reference, subtracting the reference from the spectra of an infected sample, and comparing the fingerprint regions of the resulting differential spectrum with reference spectra of bacteria in saline. Using this diagnostic technique, specific bacteria can be identified sooner and without culturing, bacteria-specific antibiotics can be prescribed sooner, resulting in decreased likelihood of antibiotic resistance and an overall reduction of medical costs.

  2. Biological and Environmental Research Advisory Committee

    Office of Science (SC) Website

    March 03-04, 2014 | U.S. DOE Office of Science (SC) March 03-04, 2014 Biological and Environmental Research Advisory Committee (BERAC) BERAC Home Meetings BERAC Minutes BERAC Minutes Archive Members Charges/Reports Charter .pdf file (135KB) BER Committees of Visitors Federal Advisory Committees BER Home Meetings Biological and Environmental Research Advisory Committee March 03-04, 2014 Print Text Size: A A A FeedbackShare Page Agenda .pdf file (21KB) Presentations: Sharlene Weatherwax .pdf

  3. Thermolysis of partial coal structures: bibenzyl and naphthols

    SciTech Connect (OSTI)

    Poutsma, M L

    1980-01-01

    All proposed processes for direct conversion of coal to synthetic liquid fuels require heating to greater than or equal to 400/sup 0/C. Exact process conditions (pyrolysis, hydropyrolysis, catalytic hydrogenation, donor solvent refining) are vital in determining the final product state (1). However, underlying all these processes may well be a set of similar thermal reactions which lead to disruption of the organic macromolecular structure of coal. Because of the structural complexity and irregularity of coal, attempts to elucidate this thermal chemistry of coal and of donor process solvents have relied heavily on thermolysis studies of model organic structures (2) and the judicious use of structure-reactivity relationships which are central to the discipline of physical organic chemistry. In this work the reaction products of the pyrolysis of bibenzyl and naphthals were identified.

  4. Biological Conversion of Sugars To Hydrocarbons | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    To Hydrocarbons Biological Conversion of Sugars To Hydrocarbons PDF explaining the biological process of bioenergy PDF icon Biological Conversion of Sugars To Hydrocarbons More Documents & Publications Catalytic Upgrading Sugars To Hydrocarbons Catalytic Upgrading of Sugars to Hydrocarbons Technology Pathway Biological Conversion of Sugars to Hydrocarbons Technology Pathway

  5. 2013 Biological Hydrogen Production Workshop Summary Report | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Biological Hydrogen Production Workshop Summary Report 2013 Biological Hydrogen Production Workshop Summary Report November 2013 summary report for the 2013 Biological Hydrogen Production Workshop. PDF icon bio_h2_workshop_final_report.pdf More Documents & Publications The Hydrogen Program at NREL: A Brief Overview Hydrogenases and Barriers for Biotechnological Hydrogen Production Technologies Renewable Hydrogen Production from Biological Systems

  6. EERE Success Story—California: Breakthrough in Algae Biology

    Broader source: Energy.gov [DOE]

    Breakthrough in algae biology will have a significant impact in the economics of algal biofuel production.

  7. Composites comprising biologically-synthesized nanomaterials

    DOE Patents [OSTI]

    Curran, Seamus; Dias, Sampath; Blau, Werner; Wang, Jun; Oremland, Ronald S; Baesman, Shaun

    2013-04-30

    The present disclosure describes composite materials containing a polymer material and a nanoscale material dispersed in the polymer material. The nanoscale materials may be biologically synthesized, such as tellurium nanorods synthesized by Bacillus selenitireducens. Composite materials of the present disclosure may have optical limiting properties and find use in optical limiting devices.

  8. Radiological/biological/aerosol removal system

    DOE Patents [OSTI]

    Haslam, Jeffery J

    2015-03-17

    An air filter replacement system for existing buildings, vehicles, arenas, and other enclosed airspaces includes a replacement air filter for replacing a standard air filter. The replacement air filter has dimensions and air flow specifications that allow it to replace the standard air filter. The replacement air filter includes a filter material that removes radiological or biological or aerosol particles.

  9. Apparatus for automated testing of biological specimens

    DOE Patents [OSTI]

    Layne, Scott P.; Beugelsdijk, Tony J.

    1999-01-01

    An apparatus for performing automated testing of infections biological specimens is disclosed. The apparatus comprise a process controller for translating user commands into test instrument suite commands, and a test instrument suite comprising a means to treat the specimen to manifest an observable result, and a detector for measuring the observable result to generate specimen test results.

  10. Modular microfluidic system for biological sample preparation

    DOE Patents [OSTI]

    Rose, Klint A.; Mariella, Jr., Raymond P.; Bailey, Christopher G.; Ness, Kevin Dean

    2015-09-29

    A reconfigurable modular microfluidic system for preparation of a biological sample including a series of reconfigurable modules for automated sample preparation adapted to selectively include a) a microfluidic acoustic focusing filter module, b) a dielectrophoresis bacteria filter module, c) a dielectrophoresis virus filter module, d) an isotachophoresis nucleic acid filter module, e) a lyses module, and f) an isotachophoresis-based nucleic acid filter.

  11. Method and apparatus for sustaining viability of biological cells on a substrate

    DOE Patents [OSTI]

    McKnight, Timothy E.; Melechko, Anatoli V.; Simpson, Michael L.

    2013-01-01

    A method for the transient transformation of a living biological cell having an intact cell membrane defining an intracellular domain, and an apparatus for the transient transformation of biological cells. The method and apparatus include introducing a compartmentalized extracellular component fixedly attached to a cellular penetrant structure to the intracellular domain of the cell, wherein the cell is fixed in a predetermined location and wherein the component is expressed within in the cell while being retained within the compartment and wherein the compartment restricts the mobility and interactions of the component within the cell and prevents transference of the component to the cell.

  12. Method and apparatus for sustaining viability of biological cells on a substrate

    DOE Patents [OSTI]

    McKnight, Timothy E. (Greenback, TN); Melechko, Anatoli V. (Oak Ridge, TN); Simpson, Michael L. (Knoxville, TN)

    2011-12-13

    A method for the transient transformation of a living biological cell having an intact cell membrane defining an intracellular domain, and an apparatus for the transient transformation of biological cells. The method and apparatus include introducing a compartmentalized extracellular component fixedly attached to a cellular penetrant structure to the intracellular domain of the cell, wherein the cell is fixed in a predetermined location and wherein the component is expressed within in the cell while being retained within the compartment and wherein the compartment restricts the mobility and interactions of the component within the cell and prevents transference of the component to the cell.

  13. Structural Materials

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Structural Materials Structural Materials Development enables advanced technologies through the discovery, development, and demonstration of cost-effective advanced structural materials for use in extreme environments (high-temperature, high-stress, erosive, and corrosive environments, including the performance of materials in contact with molten slags and salts). Research includes materials design and discovery, materials processing and manufacturing, and service-life prediction of materials

  14. Biological imaging by soft x-ray diffraction microscopy

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Shapiro, D.; Thibault, P.; Beetz, T.; Elser, V.; Howells, M.; Jacobsen, C.; Kirz, J.; Lima, E.; Miao, H.; Neiman, A. M.; et al

    2005-10-25

    We have used the method of x-ray diffraction microscopy to image the complex-valued exit wave of an intact and unstained yeast cell. The images of the freeze-dried cell, obtained by using 750-eV x-rays from different angular orientations, portray several of the cell's major internal components to 30-nm resolution. The good agreement among the independently recovered structures demonstrates the accuracy of the imaging technique. To obtain the best possible reconstructions, we have implemented procedures for handling noisy and incomplete diffraction data, and we propose a method for determining the reconstructed resolution. This work represents a previously uncharacterized application of x-ray diffractionmore » microscopy to a specimen of this complexity and provides confidence in the feasibility of the ultimate goal of imaging biological specimens at 10-nm resolution in three dimensions.« less

  15. Biology Division progress report, October 1, 1984-September 30, 1985

    SciTech Connect (OSTI)

    Not Available

    1986-01-01

    The body of this report provides summaries of the aims, scope and progress of the research by groups of investigators in the Division during the period of October 1, 1984, through September 30, 1985. At the end of each summary is a list of publications covering the same period. For convenience, the summaries are assembled under Sections in accordance with the current organizational structure of the Biology Division; each Section begins with an overview. It will be apparent, however, tha crosscurrents run throughout the Division and that the various programs support and interact with each other. In addition, this report includes information on the Division's educational activities, Advisory Committee, seminar program, and international interactions, as well as extramural activities of staff members, abstracts for technical meetings, and funding and personnel levels.

  16. Work Force Discipline--Withdrawn

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2012-04-05

    Withdrawn 4-26-14. Significant changes from the Order include the deletion of material included in other documents such as statutes and Federal regulations. In addition, an updated Table of Penalties now includes Smoking, Cyber Security, Standards of Conduct or other Ethical Violations, and the incorporation of the Travel and Purchase Card Guidance dated January 2003.

  17. 2011 Archaea: Ecology, Metabolism, & Molecular Biology

    SciTech Connect (OSTI)

    Keneth Stedman

    2011-08-05

    Archaea, one of three major evolutionary lineages of life, are a fascinating and diverse group of microbes with deep roots overlapping those of eukaryotes. The focus of the 'Archaea: Ecology Metabolism & Molecular Biology' GRC conference expands on a number of emerging topics highlighting new paradigms in archaeal metabolism, genome function and systems biology; information processing; evolution and the tree of life; the ecology and diversity of archaea and their viruses. The strength of this conference lies in its ability to couple a field with a rich history in high quality research with new scientific findings in an atmosphere of stimulating exchange. This conference remains an excellent opportunity for younger scientists to interact with world experts in this field.

  18. Monitoring Biological Activity at Geothermal Power Plants

    SciTech Connect (OSTI)

    Peter Pryfogle

    2005-09-01

    The economic impact of microbial growth in geothermal power plants has been estimated to be as high as $500,000 annually for a 100 MWe plant. Many methods are available to monitor biological activity at these facilities; however, very few plants have any on-line monitoring program in place. Metal coupon, selective culturing (MPN), total organic carbon (TOC), adenosine triphosphate (ATP), respirometry, phospholipid fatty acid (PLFA), and denaturing gradient gel electrophoresis (DGGE) characterizations have been conducted using water samples collected from geothermal plants located in California and Utah. In addition, the on-line performance of a commercial electrochemical monitor, the BIoGEORGE?, has been evaluated during extended deployments at geothermal facilities. This report provides a review of these techniques, presents data on their application from laboratory and field studies, and discusses their value in characterizing and monitoring biological activities at geothermal power plants.

  19. 2009 Archaea: Ecology, Metabolism & Molecular Biology GRC

    SciTech Connect (OSTI)

    Dr. Julie Maupin- Furlow

    2009-07-26

    Archaea, one of three major evolutionary lineages of life, are a fascinating and diverse group of microbes with deep roots overlapping those of eukaryotes. The focus of the 'Archaea: Ecology Metabolism & Molecular Biology' GRC conference expands on a number of emerging topics highlighting new paradigms in archaeal metabolism, genome function and systems biology; information processing; evolution and the tree of life; the ecology and diversity of archaea and their viruses; and industrial applications. The strength of this conference lies in its ability to couple a field with a rich history in high quality research with new scientific findings in an atmosphere of stimulating exchange. This conference remains an excellent opportunity for younger scientists to interact with world experts in this field.

  20. Developing Biological Specifications for Fish Friendly Turbines

    SciTech Connect (OSTI)

    Neitzel, Duane A.

    2009-09-14

    This factsheet explains studies conducted in a highly reproducible manner to examine the biological effects to fish exposed to a shear environment in the laboratory. Strain rate was used as the index of intensity to describe the hydraulic force experienced by a fish in a shear environment. It was determined that no significant injuries occurred to any fish subjected to strain rates equal to or less than 500 cm/s/cm.

  1. KPFM and PFM of Biological Systems

    SciTech Connect (OSTI)

    Rodriguez, Brian [University College, Dublin; Kalinin, Sergei V [ORNL

    2011-01-01

    Surface potentials and electrostatic interactions in biological systems are a key element of cellular regulation and interaction. Examples include cardiac and muscular activity, voltage-gated ion channels, protein folding and assembly, and electroactive cells and electrotransduction. The coupling between electrical, mechanical, and chemical signals and responses in cellular systems necessitates the development of tools capable of measuring the distribution of charged species, surface potentials, and mechanical responses to applied electrical stimuli and vice versa, ultimately under physiological conditions. In this chapter, applications of voltage-modulated atomic force microscopy (AFM) methods including Kelvin probe force microscopy (KPFM) and piezoresponse force microscopy (PFM) to biological systems are discussed. KPFM is a force-sensitive non-contact or intermittent-contact mode AFM technique that allows electrostatic interactions and surface potentials to be addressed. Beyond long-range electrostatic interactions, the application of bias can lead to a mechanical response, e.g., due to linear piezoelectric coupling in polar biopolymers or via more complex electrotransduction and redox pathways in other biosystems. The use and development of PFM, based on direct electromechanical detection, to biological systems will also be addressed. The similarities and limitations of measuring surface potentials and electromechanical coupling in solution will be outlined.

  2. Computational Tools to Assess Turbine Biological Performance

    SciTech Connect (OSTI)

    Richmond, Marshall C.; Serkowski, John A.; Rakowski, Cynthia L.; Strickler, Brad; Weisbeck, Molly; Dotson, Curtis L.

    2014-07-24

    Public Utility District No. 2 of Grant County (GCPUD) operates the Priest Rapids Dam (PRD), a hydroelectric facility on the Columbia River in Washington State. The dam contains 10 Kaplan-type turbine units that are now more than 50 years old. Plans are underway to refit these aging turbines with new runners. The Columbia River at PRD is a migratory pathway for several species of juvenile and adult salmonids, so passage of fish through the dam is a major consideration when upgrading the turbines. In this paper, a method for turbine biological performance assessment (BioPA) is demonstrated. Using this method, a suite of biological performance indicators is computed based on simulated data from a CFD model of a proposed turbine design. Each performance indicator is a measure of the probability of exposure to a certain dose of an injury mechanism. Using known relationships between the dose of an injury mechanism and frequency of injury (dose–response) from laboratory or field studies, the likelihood of fish injury for a turbine design can be computed from the performance indicator. By comparing the values of the indicators from proposed designs, the engineer can identify the more-promising alternatives. We present an application of the BioPA method for baseline risk assessment calculations for the existing Kaplan turbines at PRD that will be used as the minimum biological performance that a proposed new design must achieve.

  3. Beamline 12.3.1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    1 Print Structurally Integrated Biology for Life Sciences (SIBYLS) Scientific discipline: Structural biology GENERAL BEAMLINE INFORMATION Operational Yes Source characteristics Superbend Energy range 5-17 keV (1% max flux) Frequency range 2.5-0.73 Angstrom wavelength Beam size 100 µm round beam default 10 µm and 30 µm collimators available for small samples with flux reduced to 1% and 12%, respectively. 120 µm x 120 µm Gaussian uncollimated beam shape at sample. Scientific discipline

  4. Beamline 12.3.1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    1 Print Structurally Integrated Biology for Life Sciences (SIBYLS) Scientific discipline: Structural biology GENERAL BEAMLINE INFORMATION Operational Yes Source characteristics Superbend Energy range 5-17 keV (1% max flux) Frequency range 2.5-0.73 Angstrom wavelength Beam size 100 µm round beam default 10 µm and 30 µm collimators available for small samples with flux reduced to 1% and 12%, respectively. 120 µm x 120 µm Gaussian uncollimated beam shape at sample. Scientific discipline

  5. Beamline 12.3.1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    3.1 Print Structurally Integrated Biology for Life Sciences (SIBYLS) Scientific discipline: Structural biology GENERAL BEAMLINE INFORMATION Operational Yes Source characteristics Superbend Energy range 5-17 keV (1% max flux) Frequency range 2.5-0.73 Angstrom wavelength Beam size 100 µm round beam default 10 µm and 30 µm collimators available for small samples with flux reduced to 1% and 12%, respectively. 120 µm x 120 µm Gaussian uncollimated beam shape at sample. Scientific discipline

  6. Beamline 12.3.1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    12.3.1 Print Structurally Integrated Biology for Life Sciences (SIBYLS) Scientific discipline: Structural biology GENERAL BEAMLINE INFORMATION Operational Yes Source characteristics Superbend Energy range 5-17 keV (1% max flux) Frequency range 2.5-0.73 Angstrom wavelength Beam size 100 µm round beam default 10 µm and 30 µm collimators available for small samples with flux reduced to 1% and 12%, respectively. 120 µm x 120 µm Gaussian uncollimated beam shape at sample. Scientific discipline

  7. Beamline 12.3.1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    1 Print Structurally Integrated Biology for Life Sciences (SIBYLS) Scientific discipline: Structural biology GENERAL BEAMLINE INFORMATION Operational Yes Source characteristics Superbend Energy range 5-17 keV (1% max flux) Frequency range 2.5-0.73 Angstrom wavelength Beam size 100 µm round beam default 10 µm and 30 µm collimators available for small samples with flux reduced to 1% and 12%, respectively. 120 µm x 120 µm Gaussian uncollimated beam shape at sample. Scientific discipline

  8. Beamline 12.3.1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    1 Print Structurally Integrated Biology for Life Sciences (SIBYLS) Scientific discipline: Structural biology GENERAL BEAMLINE INFORMATION Operational Yes Source characteristics Superbend Energy range 5-17 keV (1% max flux) Frequency range 2.5-0.73 Angstrom wavelength Beam size 100 µm round beam default 10 µm and 30 µm collimators available for small samples with flux reduced to 1% and 12%, respectively. 120 µm x 120 µm Gaussian uncollimated beam shape at sample. Scientific discipline

  9. Beamline 12.3.1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    1 Print Structurally Integrated Biology for Life Sciences (SIBYLS) Scientific discipline: Structural biology GENERAL BEAMLINE INFORMATION Operational Yes Source characteristics Superbend Energy range 5-17 keV (1% max flux) Frequency range 2.5-0.73 Angstrom wavelength Beam size 100 µm round beam default 10 µm and 30 µm collimators available for small samples with flux reduced to 1% and 12%, respectively. 120 µm x 120 µm Gaussian uncollimated beam shape at sample. Scientific discipline

  10. Beamline 12.3.1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    1 Print Structurally Integrated Biology for Life Sciences (SIBYLS) Scientific discipline: Structural biology GENERAL BEAMLINE INFORMATION Operational Yes Source characteristics Superbend Energy range 5-17 keV (1% max flux) Frequency range 2.5-0.73 Angstrom wavelength Beam size 100 µm round beam default 10 µm and 30 µm collimators available for small samples with flux reduced to 1% and 12%, respectively. 120 µm x 120 µm Gaussian uncollimated beam shape at sample. Scientific discipline

  11. Beamline 12.3.1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    1 Print Structurally Integrated Biology for Life Sciences (SIBYLS) Scientific discipline: Structural biology GENERAL BEAMLINE INFORMATION Operational Yes Source characteristics Superbend Energy range 5-17 keV (1% max flux) Frequency range 2.5-0.73 Angstrom wavelength Beam size 100 µm round beam default 10 µm and 30 µm collimators available for small samples with flux reduced to 1% and 12%, respectively. 120 µm x 120 µm Gaussian uncollimated beam shape at sample. Scientific discipline

  12. Beamline 12.3.1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    1 Print Structurally Integrated Biology for Life Sciences (SIBYLS) Scientific discipline: Structural biology GENERAL BEAMLINE INFORMATION Operational Yes Source characteristics Superbend Energy range 5-17 keV (1% max flux) Frequency range 2.5-0.73 Angstrom wavelength Beam size 100 µm round beam default 10 µm and 30 µm collimators available for small samples with flux reduced to 1% and 12%, respectively. 120 µm x 120 µm Gaussian uncollimated beam shape at sample. Scientific discipline

  13. Chemical and biological sensing using tuning forks

    DOE Patents [OSTI]

    Tao, Nongjian; Boussaad, Salah

    2012-07-10

    A device for sensing a chemical analyte is disclosed. The device is comprised of a vibrating structure having first and second surfaces and having an associated resonant frequency and a wire coupled between the first and second surfaces of the vibrating structure, wherein the analyte interacts with the wire and causes a change in the resonant frequency of the vibrating structure. The vibrating structure can include a tuning fork. The vibrating structure can be comprised of quartz. The wire can be comprised of polymer. A plurality of vibrating structures are arranged in an array to increase confidence by promoting a redundancy of measurement or to detect a plurality of chemical analytes. A method of making a device for sensing a chemical analyte is also disclosed.

  14. Structural Evolution

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    (Journal Article) | SciTech Connect Structural Basis of UV DNA-Damage Recognition by the DDB1-DDB2 Complex Citation Details In-Document Search Title: Structural Basis of UV DNA-Damage Recognition by the DDB1-DDB2 Complex Ultraviolet (UV) light-induced pyrimidine photodimers are repaired by the nucleotide excision repair pathway. Photolesions have biophysical parameters closely resembling undamaged DNA, impeding discovery through damage surveillance proteins. The DDB1DDB2 complex serves in

  15. Biological Imaging by Soft X-Ray Diffraction Microscopy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Biological Imaging by Soft X-Ray Diffraction Microscopy Biological Imaging by Soft X-Ray Diffraction Microscopy Print Wednesday, 30 November 2005 00:00 Electron and x-ray...

  16. BioLogical Capital BLC | Open Energy Information

    Open Energy Info (EERE)

    BioLogical Capital BLC Jump to: navigation, search Name: BioLogical Capital (BLC) Place: Denver, Colorado Zip: 80202 Product: Denver Colorado-based group focusing on generating...

  17. Systems Biology in Prokaryote - Eukaryote Symbiosis | Stanford...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    vibrational structures; but, information about the latter two are not accessible by diffraction methods. Fortunately, spectroscopic methods provide data to help resolve...

  18. Organizational Structure | Photosynthetic Antenna Research Center

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Organizational Structure Organizational Structure

  19. Breaking the Biological Barriers to Cellulosic Ethanol, June 2006 |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Breaking the Biological Barriers to Cellulosic Ethanol, June 2006 Breaking the Biological Barriers to Cellulosic Ethanol, June 2006 Breaking the Biological Barriers to Cellulosic Ethanol, June 2006 PDF icon b2blowres63006.pdf More Documents & Publications Breaking the Biological Barriers to Cellulosic Ethanol, June 2006 Review of Recent Pilot Scale Cellulosic Ethanol Demonstration Biochemical Conversion: Using Hydrolysis, Fermentation, and Catalysis to Make Fuels and

  20. Biological Conversion of Sugars to Hydrocarbons Technology Pathway

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... to leverage experience in biochemical processing, specifically cellulose and ... in downstream biological conversion and improving overall process integration. ...

  1. AN INTEGRATED BIOLOGICAL CONTROL SYSTEM AT HANFORD

    SciTech Connect (OSTI)

    JOHNSON AR; CAUDILL JG; GIDDINGS RF; RODRIGUEZ JM; ROOS RC; WILDE JW

    2010-02-11

    In 1999 an integrated biological control system was instituted at the U.S. Department of Energy's Hanford Site. Successes and changes to the program needed to be communicated to a large and diverse mix of organizations and individuals. Efforts at communication are directed toward the following: Hanford Contractors (Liquid or Tank Waste, Solid Waste, Environmental Restoration, Science and Technology, Site Infrastructure), General Hanford Employees, and Hanford Advisory Board (Native American Tribes, Environmental Groups, Local Citizens, Washington State and Oregon State regulatory agencies). Communication was done through direct interface meetings, individual communication, where appropriate, and broadly sharing program reports. The objectives of the communication efforts was to have the program well coordinated with Hanford contractors, and to have the program understood well enough that all stakeholders would have confidence in the work performed by the program to reduce or elimated spread of radioactive contamination by biotic vectors. Communication of successes and changes to an integrated biological control system instituted in 1999 at the Department of Energy's Hanford Site have required regular interfaces with not only a diverse group of Hanford contractors (i.e., those responsible for liquid or tank waste, solid wastes, environmental restoration, science and technology, and site infrastructure), and general Hanford employees, but also with a consortium of designated stake holders organized as the Hanford Advisory Board (i.e., Native American tribes, various environmental groups, local citizens, Washington state and Oregon regulatory agencies, etc.). Direct interface meetings, individual communication where appropriate, and transparency of the biological control program were the methods and outcome of this effort.

  2. LABORATORY OF NUCLEAR MEDICINE AND RADIATION BIOLOGY

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    MEDICINE AND RADIATION BIOLOGY 900 VETERAN AVENUE UNIVERSITY OF CALIFORNIA, LOS ANGELES, CALIFORNIA 90024 AND DEPARTMENT OF RADIOLOGICAL SCIENCES UCLA SCHOOL OF MEDICINE, LOS ANGELES, CALIFORNIA 90024 This manuscript is a contribution to the monograph edited by Daniel S. Berman and Dean Mason, entitled "Clinical Nuclear Cardiology". These studies were supported by Contract #DE-AM03-76-SF00012 between the U.S. Department of Energy and the University of California Prepared for the U.S.

  3. LANSCE | Lujan Center | Biology Preparation Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Biology Preparation Laboratory The Lujan Center Biolab offers a variety of capabilities. 1) Biodeuteration Lab (BDL) We run a protein expression lab for perdeuteration of user proteins. We offer full perdeuteration (~99%) using our algal-based media for bacterial growth. We also have M9 minimal media made in D2O for expression of up to ~85% perdeuteration. Users can use our lab in person or mail-in a plasmid for us to express for them. We also have standard protein expression equipment:

  4. Historical Information H.2 Biological Studies

    Office of Legacy Management (LM)

    _-_ . - H.2 Biological Studies (0 \ j ; : : j Book . l Ad Hoc Rulison Review Panel Comments Regarding Re-Entry , and Testing Operations, December 22, 1969 This page intentionally left blank December 22, 1969 M r . Robert E. Miller, Manager Nevada Operations Office U. S. Atomic Energy Commission Post Office Box 14100 Las Vegas, Nevada 89114 Dear M r . Miller: F4cmbers of t h e A d Hoc Rulison Review Panel reconvened on December 22, 1969, t o hear and discuss comments r e s u l t i n g from our

  5. Synchronous Behavior of Two Coupled Biological Neurons

    SciTech Connect (OSTI)

    Elson, R.C.; Selverston, A.I.; Elson, R.C.; Selverston, A.I.; Huerta, R.; Rulkov, N.F.; Rabinovich, M.I.; Abarbanel, H.D.; Selverston, A.I.; Huerta, R.; Abarbanel, H.D.

    1998-12-01

    We report experimental studies of synchronization phenomena in a pair of biological neurons that interact through naturally occurring, electrical coupling. When these neurons generate irregular bursts of spikes, the natural coupling synchronizes slow oscillations of membrane potential, but not the fast spikes. By adding artificial electrical coupling we studied transitions between synchrony and asynchrony in both slow oscillations and fast spikes. We discuss the dynamics of bursting and synchronization in living neurons with distributed functional morphology. {copyright} {ital 1998} {ital The American Physical Society}

  6. Third international congress of plant molecular biology: Molecular biology of plant growth and development

    SciTech Connect (OSTI)

    Hallick, R.B.

    1995-02-01

    The Congress was held October 6-11, 1991 in Tucson with approximately 3000 scientists attending and over 300 oral presentations and 1800 posters. Plant molecular biology is one of the most rapidly developing areas of the biological sciences. Recent advances in the ability to isolate genes, to study their expression, and to create transgenic plants have had a major impact on our understanding of the many fundamental plant processes. In addition, new approaches have been created to improve plants for agricultural purposes. This is a book of presentation and posters from the conference.

  7. Biological and Environmental Research Network Requirements

    SciTech Connect (OSTI)

    Balaji, V.; Boden, Tom; Cowley, Dave; Dart, Eli; Dattoria, Vince; Desai, Narayan; Egan, Rob; Foster, Ian; Goldstone, Robin; Gregurick, Susan; Houghton, John; Izaurralde, Cesar; Johnston, Bill; Joseph, Renu; Kleese-van Dam, Kerstin; Lipton, Mary; Monga, Inder; Pritchard, Matt; Rotman, Lauren; Strand, Gary; Stuart, Cory; Tatusova, Tatiana; Tierney, Brian; Thomas, Brian; Williams, Dean N.; Zurawski, Jason

    2013-09-01

    The Energy Sciences Network (ESnet) is the primary provider of network connectivity for the U.S. Department of Energy (DOE) Office of Science (SC), the single largest supporter of basic research in the physical sciences in the United States. In support of SC programs, ESnet regularly updates and refreshes its understanding of the networking requirements of the instruments, facilities, scientists, and science programs that it serves. This focus has helped ESnet be a highly successful enabler of scientific discovery for over 25 years. In November 2012, ESnet and the Office of Biological and Environmental Research (BER) of the DOE SC organized a review to characterize the networking requirements of the programs funded by the BER program office. Several key findings resulted from the review. Among them: 1) The scale of data sets available to science collaborations continues to increase exponentially. This has broad impact, both on the network and on the computational and storage systems connected to the network. 2) Many science collaborations require assistance to cope with the systems and network engineering challenges inherent in managing the rapid growth in data scale. 3) Several science domains operate distributed facilities that rely on high-performance networking for success. Key examples illustrated in this report include the Earth System Grid Federation (ESGF) and the Systems Biology Knowledgebase (KBase). This report expands on these points, and addresses others as well. The report contains a findings section as well as the text of the case studies discussed at the review.

  8. Acceptance Criteria Framework for Autonomous Biological Detectors

    SciTech Connect (OSTI)

    Dzenitis, J M

    2006-12-12

    The purpose of this study was to examine a set of user acceptance criteria for autonomous biological detection systems for application in high-traffic, public facilities. The test case for the acceptance criteria was the Autonomous Pathogen Detection System (APDS) operating in high-traffic facilities in New York City (NYC). However, the acceptance criteria were designed to be generally applicable to other biological detection systems in other locations. For such detection systems, ''users'' will include local authorities (e.g., facility operators, public health officials, and law enforcement personnel) and national authorities [including personnel from the Department of Homeland Security (DHS), the BioWatch Program, the Centers for Disease Control and Prevention (CDC), and the Federal Bureau of Investigation (FBI)]. The panel members brought expertise from a broad range of backgrounds to complete this picture. The goals of this document are: (1) To serve as informal guidance for users in considering the benefits and costs of these systems. (2) To serve as informal guidance for developers in understanding the needs of users. In follow-up work, this framework will be used to systematically document the APDS for appropriateness and readiness for use in NYC.

  9. Nanocrystal structures

    DOE Patents [OSTI]

    Eisler, Hans J. (Stoneham, MA); Sundar, Vikram C. (Stoneham, MA); Walsh, Michael E. (Everett, MA); Klimov, Victor I. (Los Alamos, NM); Bawendi, Moungi G. (Cambridge, MA); Smith, Henry I. (Sudbury, MA)

    2008-12-30

    A structure including a grating and a semiconductor nanocrystal layer on the grating, can be a laser. The semiconductor nanocrystal layer can include a plurality of semiconductor nanocrystals including a Group II-VI compound, the nanocrystals being distributed in a metal oxide matrix. The grating can have a periodicity from 200 nm to 500 nm.

  10. Nanocrystal structures

    DOE Patents [OSTI]

    Eisler, Hans J.; Sundar, Vikram C.; Walsh, Michael E.; Klimov, Victor I.; Bawendi, Moungi G.; Smith, Henry I.

    2006-12-19

    A structure including a grating and a semiconductor nanocrystal layer on the grating, can be a laser. The semiconductor nanocrystal layer can include a plurality of semiconductor nanocrystals including a Group II–VI compound, the nanocrystals being distributed in a metal oxide matrix. The grating can have a periodicity from 200 nm to 500 nm.

  11. Nanocrystal structures

    DOE Patents [OSTI]

    Eisler, Hans J.; Sundar, Vikram C.; Walsh, Michael E.; Klimov, Victor I.; Bawendi, Moungi G.; Smith, Henry I.

    2006-12-19

    A structure including a grating and a semiconductor nanocrystal layer on the grating, can be a laser. The semiconductor nanocrystal layer can include a plurality of semiconductor nanocrystals including a Group IIVI compound, the nanocrystals being distributed in a metal oxide matrix. The grating can have a periodicity from 200 nm to 500 nm.

  12. ANAEROBIC BIOLOGICAL TREATMENT OF PRODUCED WATER

    SciTech Connect (OSTI)

    John R. Gallagher

    2001-07-31

    During the production of oil and gas, large amounts of water are brought to the surface and must be disposed of in an environmentally sensitive manner. This is an especially difficult problem in offshore production facilities where space is a major constraint. The chief regulatory criterion for produced water is oil and grease. Most facilities have little trouble meeting this criterion using conventional oil-water separation technologies. However, some operations have significant amounts of naphthenic acids in the water that behave as oil and grease but are not well removed by conventional technologies. Aerobic biological treatment of naphthenic acids in simulated-produced water has been demonstrated by others; however, the system was easily overloaded by the large amounts of low-molecular-weight organic acids often found in produced waters. The objective of this research was to determine the ability of an anaerobic biological system to treat these organic acids in a simulated produced water and to examine the potential for biodegradation of the naphthenic acids in the anaerobic environment. A small fixed-film anaerobic biological reactor was constructed and adapted to treat a simulated produced water. The bioreactor was tubular, with a low-density porous glass packing material. The inocula to the reactor was sediment from a produced-water holding pond from a municipal anaerobic digester and two salt-loving methanogenic bacteria. During start-up, the feed to the reactor contained glucose as well as typical produced-water components. When glucose was used, rapid gas production was observed. However, when glucose was eliminated and the major organic component was acetate, little gas was generated. Methane production from acetate may have been inhibited by the high salt concentrations, by sulfide, or because of the lack, despite seeding, of microbes capable of converting acetate to methane. Toluene, a minor component of the produced water (0.1 g/L) was removed in the reactor. Batch tests were conducted to examine naphthenic acid biodegradability under several conditions. The conditions used were seed from the anaerobic reactor, wetland sediments under aerobic and anaerobic conditions, and a sterile control. The naphthenic acid was from a commercial source isolated from Gulf Coast petroleum as was dosed at 2 mg/mL. The incubations were for 30 days at 30 C. The results showed that the naphthenic acids were not biodegraded under anaerobic conditions, but were degraded under aerobic conditions. Despite poor performance of the anaerobic reactor, it remains likely that anaerobic treatment of acetate, toluene, and, potentially, other produced-water components is feasible.

  13. Biology and Medicine Division annual report, 1985

    SciTech Connect (OSTI)

    Not Available

    1986-04-01

    This book briefly describes the activities of the Biology and Medicine Division of the Lawrence Berkeley Laboratory. During the past year the Donner Pavilion program on the treatment of arteriovenous malformations in the brain has chalked up very significant successes. The disease control rate has been high and objective measures of success using cerebral angiography have been established. The new high resolution positron emitting tomographic imager has been demonstrated to operate successfully. In the Radiation Biophysics program, the availability of higher mass ions up to uranium has allowed us cell and tissue studies in a radiation domain that is entirely new. Using uranium beams, investigators have already made new and exciting findings that are described in the body of the report.

  14. Overview of selected molecular biological databases

    SciTech Connect (OSTI)

    Rayl, K.D.; Gaasterland, T.

    1994-11-01

    This paper presents an overview of the purpose, content, and design of a subset of the currently available biological databases, with an emphasis on protein databases. Databases included in this summary are 3D-ALI, Berlin RNA databank, Blocks, DSSP, EMBL Nucleotide Database, EMP, ENZYME, FSSP, GDB, GenBank, HSSP, LiMB, PDB, PIR, PKCDD, ProSite, and SWISS-PROT. The goal is to provide a starting point for researchers who wish to take advantage of the myriad available databases. Rather than providing a complete explanation of each database, we present its content and form by explaining the details of typical entries. Pointers to more complete ``user guides`` are included, along with general information on where to search for a new database.

  15. Quantifying evolvability in small biological networks

    SciTech Connect (OSTI)

    Nemenman, Ilya; Mugler, Andrew; Ziv, Etay; Wiggins, Chris H

    2008-01-01

    The authors introduce a quantitative measure of the capacity of a small biological network to evolve. The measure is applied to a stochastic description of the experimental setup of Guet et al. (Science 2002, 296, pp. 1466), treating chemical inducers as functional inputs to biochemical networks and the expression of a reporter gene as the functional output. The authors take an information-theoretic approach, allowing the system to set parameters that optimise signal processing ability, thus enumerating each network's highest-fidelity functions. All networks studied are highly evolvable by the measure, meaning that change in function has little dependence on change in parameters. Moreover, each network's functions are connected by paths in the parameter space along which information is not significantly lowered, meaning a network may continuously change its functionality without completely losing it along the way. This property further underscores the evolvability of the networks.

  16. Recovery from chemical, biological, and radiological incidents :

    SciTech Connect (OSTI)

    Franco, David Oliver; Yang, Lynn I.; Hammer, Ann E.

    2012-06-01

    To restore regional lifeline services and economic activity as quickly as possible after a chemical, biological or radiological incident, emergency planners and managers will need to prioritize critical infrastructure across many sectors for restoration. In parallel, state and local governments will need to identify and implement measures to promote reoccupation and economy recovery in the region. This document provides guidance on predisaster planning for two of the National Disaster Recovery Framework Recovery Support Functions: Infrastructure Systems and Economic Recovery. It identifies key considerations for infrastructure restoration, outlines a process for prioritizing critical infrastructure for restoration, and identifies critical considerations for promoting regional economic recovery following a widearea disaster. Its goal is to equip members of the emergency preparedness community to systematically prioritize critical infrastructure for restoration, and to develop effective economic recovery plans in preparation for a widearea CBR disaster.

  17. Terminal structure

    DOE Patents [OSTI]

    Schmidt, Frank; Allais, Arnaud; Mirebeau, Pierre; Ganhungu, Francois; Lallouet, Nicolas

    2009-10-20

    A terminal structure (2) for a superconducting cable (1) is described. It consists of a conductor (2a) and an insulator (2b) that surrounds the conductor (2a), wherein the superconducting cable (1) has a core with a superconducting conductor (5) and a layer of insulation that surrounds the conductor (5), and wherein the core is arranged in such a way that it can move longitudinally in a cryostat. The conductor (2a) of the terminal structure (2) is electrically connected with the superconducting conductor (5) or with a normal conductor (6) that is connected with the superconducting conductor (5) by means of a tubular part (7) made of an electrically conductive material, wherein the superconducting conductor (5) or the normal conductor (6) can slide in the part (7) in the direction of the superconductor.

  18. Superconducting structure

    DOE Patents [OSTI]

    Kwon, Chuhee; Jia, Quanxi; Foltyn, Stephen R.

    2003-04-01

    A superconductive structure including a dielectric oxide substrate, a thin buffer layer of a superconducting material thereon; and, a layer of a rare earth-barium-copper oxide superconducting film thereon the thin layer of yttrium-barium-copper oxide, the rare earth selected from the group consisting of samarium, gadolinium, ytterbium, erbium, neodymium, dysprosium, holmium, lutetium, a combination of more than one element from the rare earth group and a combination of one or more elements from the rare earth group with yttrium, the buffer layer of superconducting material characterized as having chemical and structural compatibility with the dielectric oxide substrate and the rare earth-barium-copper oxide superconducting film is provided.

  19. Superconducting Structure

    DOE Patents [OSTI]

    Kwon, Chuhee; Jia, Quanxi; Foltyn, Stephen R.

    2005-09-13

    A superconductive structure including a dielectric oxide substrate, a thin buffer layer of a superconducting material thereon; and, a layer of a rare earth-barium-copper oxide superconducting film thereon the thin layer of yttrium-barium-copper oxide, the rare earth selected from the group consisting of samarium, gadolinium, ytterbium, erbium, neodymium, dysprosium, holmium, lutetium, a combination of more than one element from the rare earth group and a combination of one or more elements from the rare earth group with yttrium, the buffer layer of superconducting material characterized as having chemical and structural compatibility with the dielectric oxide substrate and the rare earth-barium-copper oxide superconducting film is provided.

  20. Plant biology research and training for the 21st century

    SciTech Connect (OSTI)

    Kelly, K.

    1992-01-01

    The committee was assembled in response to a request from the National Science Foundation (NSF), the US Department of Agriculture (USDA), and the US Department of Energy (DoE). The leadership of these agencies asked the National Academy of Sciences through the National Research Council (NRC) to assess the status of plant-science research in the United States in light of the opportunities arising from advances inother areas of biology. NRC was asked to suggest ways of accelerating the application of these new biologic concepts and tools to research in plant science with the aim of enhancing the acquisition of new knowledge about plants. The charge to the committee was to examine the following: Organizations, departments, and institutions conducting plant biology research; human resources involved in plant biology research; graduate training programs in plant biology; federal, state, and private sources of support for plant-biology research; the role of industry in conducting and supporting plant-biology research; the international status of US plant-biology research; and the relationship of plant biology to leading-edge research in biology.

  1. Plant biology research and training for the 21st century

    SciTech Connect (OSTI)

    Kelly, K.

    1992-12-31

    The committee was assembled in response to a request from the National Science Foundation (NSF), the US Department of Agriculture (USDA), and the US Department of Energy (DoE). The leadership of these agencies asked the National Academy of Sciences through the National Research Council (NRC) to assess the status of plant-science research in the United States in light of the opportunities arising from advances inother areas of biology. NRC was asked to suggest ways of accelerating the application of these new biologic concepts and tools to research in plant science with the aim of enhancing the acquisition of new knowledge about plants. The charge to the committee was to examine the following: Organizations, departments, and institutions conducting plant biology research; human resources involved in plant biology research; graduate training programs in plant biology; federal, state, and private sources of support for plant-biology research; the role of industry in conducting and supporting plant-biology research; the international status of US plant-biology research; and the relationship of plant biology to leading-edge research in biology.

  2. Bioforensics: Characterization of biological weapons agents by NanoSIMS

    SciTech Connect (OSTI)

    Weber, P K; Ghosal, S; Leighton, T J; Wheeler, K E; Hutcheon, I D

    2007-02-26

    The anthrax attacks of Fall 2001 highlight the need to develop forensic methods based on multiple identifiers to determine the origin of biological weapons agents. Genetic typing methods (i.e., DNA and RNA-based) provide one attribution technology, but genetic information alone is not usually sufficient to determine the provenance of the material. Non-genetic identifiers, including elemental and isotopic signatures, provide complementary information that can be used to identify the means, geographic location and date of production. Under LDRD funding, we have successfully developed the techniques necessary to perform bioforensic characterization with the NanoSIMS at the individual spore level. We have developed methods for elemental and isotopic characterization at the single spore scale. We have developed methods for analyzing spore sections to map elemental abundance within spores. We have developed rapid focused ion beam (FIB) sectioning techniques for spores to preserve elemental and structural integrity. And we have developed a high-resolution depth profiling method to characterize the elemental distribution in individual spores without sectioning. We used these newly developed methods to study the controls on elemental abundances in spores, characterize the elemental distribution of in spores, and to study elemental uptake by spores. Our work under this LDRD project attracted FBI and DHS funding for applied purposes.

  3. Nonlinear, noniterative, single-distance phase retrieval and developmental biology

    SciTech Connect (OSTI)

    Moosmann, Julian; Altapova, Venera; Haenschke, Daniel; Hofmann, Ralf; Baumbach, Tilo

    2012-05-17

    For coherent X-ray imaging, based on phase contrast through free-space Fresnel propagation, we discuss two noniterative, nonlinear approaches to the phase-retrieval problem from a single-distance intensity map of a pure-phase object. On one hand, a perturbative set-up is proposed where nonlinear corrections to the linearized transport-of-intensity situation are expanded in powers of the object-detector distance z and are evaluated in terms of the linear estimate. On the other hand, a nonperturbative projection algorithm, which is based on the (linear and local) contrast-transfer function (CTF), works with an effective phase in Fourier space. This effective phase obeys a modified CTF relation between intensity contrast at z > 0 and phase contrast at z= 0: Unphysical singularities of the local CTF model are cut off to yield 'quasiparticles' in analogy to the theory of the Fermi liquid. By identifying the positions of the zeros of the Fourier transformed intensity contrast as order parameters for the dynamical breaking of scaling symmetry we investigate the phase structure of the forward-propagation problem when interpreted as a statistical system. Results justify the quasiparticle approach for a wide range of intermediary phase variations. The latter algorithm is applied to data from biological samples recorded at the beamlines TopoTomo and ID19 at ANKA and ESRF, respectively.

  4. Methods of biological dosimetry employing chromosome-specific staining

    DOE Patents [OSTI]

    Gray, Joe W.; Pinkel, Daniel

    2000-01-01

    Methods and compositions for staining based upon nucleic acid sequence that employ nucleic acid probes are provided. Said methods produce staining patterns that can be tailored for specific cytogenetic analyses. Said probes are appropriate for in situ hybridization and stain both interphase and metaphase chromosomal material with reliable signals. The nucleic acid probes are typically of a complexity greater than 50 kb, the complexity depending upon the cytogenetic application. Methods are provided to disable the hybridization capacity of shared, high copy repetitive sequences and/or remove such sequences to provide for useful contrast. Still further methods are provided to produce chromosome-specific staining reagents which are made specific to the targeted chromosomal material, which can be one or more whole chromosomes, one or more regions on one or more chromosomes, subsets of chromosomes and/or the entire genome. Probes and test kits are provided for use in tumor cytogenetics, in the detection of disease related loci, in analysis of structural abnormalities, such as translocations, and for biological dosimetry. Further, methods and prenatal test kits are provided to stain targeted chromosomal material of fetal cells, including fetal cells obtained from maternal blood. Still further, the invention provides for automated means to detect and analyse chromosomal abnormalities.

  5. Modular microfluidic system for biological sample preparation (Patent) |

    Office of Scientific and Technical Information (OSTI)

    SciTech Connect Modular microfluidic system for biological sample preparation Citation Details In-Document Search Title: Modular microfluidic system for biological sample preparation A reconfigurable modular microfluidic system for preparation of a biological sample including a series of reconfigurable modules for automated sample preparation adapted to selectively include a) a microfluidic acoustic focusing filter module, b) a dielectrophoresis bacteria filter module, c) a dielectrophoresis

  6. Biological Conversion of Sugars to Hydrocarbons Technology Pathway

    Office of Scientific and Technical Information (OSTI)

    (Technical Report) | SciTech Connect Biological Conversion of Sugars to Hydrocarbons Technology Pathway Citation Details In-Document Search Title: Biological Conversion of Sugars to Hydrocarbons Technology Pathway This technology pathway case investigates the biological conversion of biomass-derived sugars to hydrocarbon biofuels, utilizing data from recent literature references and information consistent with recent pilot-scale demonstrations at NREL. Technical barriers and key research

  7. Deadline Extended for RFI on Biological Hydrogen Production | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Deadline Extended for RFI on Biological Hydrogen Production Deadline Extended for RFI on Biological Hydrogen Production February 26, 2014 - 12:00am Addthis DOE has extended the submission deadline for this Request for Information. Responses must be submitted by 5:00 p.m. Eastern Time on March 14, 2014. The U.S. Department of Energy's (DOE's) Fuel Cell Technologies Office has issued a request for information (RFI) seeking feedback from interested stakeholders regarding biological

  8. Implications of structural genomics target selection strategies: Pfam5000,

    Office of Scientific and Technical Information (OSTI)

    whole genome, and random approaches (Journal Article) | SciTech Connect Implications of structural genomics target selection strategies: Pfam5000, whole genome, and random approaches Citation Details In-Document Search Title: Implications of structural genomics target selection strategies: Pfam5000, whole genome, and random approaches The structural genomics project is an international effort to determine the three-dimensional shapes of all important biological macromolecules, with a primary

  9. Borrowing from Nature to Produce Highly Structured Biomimetic Materials

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Borrowing from Nature to Produce Highly Structured Biomimetic Materials Borrowing from Nature to Produce Highly Structured Biomimetic Materials Print Wednesday, 27 June 2012 00:00 Doing Nature One Better Biological tissues such as those that make up our organs are amazing feats of structural engineering. Even more fantastic, the tissues assemble themselves from building blocks based on the proteins that cells synthesize without any outside direction. And, if this isn't mind-boggling enough,

  10. WILD PIGS: BIOLOGY, DAMAGE, CONTROL TECHINQUES AND MANAGEMENT

    SciTech Connect (OSTI)

    Mayer, John; Brisbin, I. Lehr

    2009-12-31

    The existence of problems with wild pigs (Sus scrofa) is nothing new to the Western Hemisphere. Damage by these introduced animals was reported as far back as 1505 by the early Spanish colonies in the Caribbean, where wild pigs were killing the colonists cattle. Droves of these animals also ravaged cultivated crops of maize and sugarcane on islands in the West Indies during this same time period. These wild pigs reportedly were very aggressive and often attacked Spanish soldiers hunting rebellious Indians or escaped slaves on these islands, especially when these animals were cornered. The documentation of such impacts by introduced populations of this species in the United States has subsequently increased in recent years, and continued up through the present (Towne and Wentworth. 1950, Wood and Barrett 1979, Mayer and Brisbin 1991, Dickson et al. 2001). In spite of a fairly constant history in this country since the early 1900s, wild pigs have had a dramatic recent increase in both distribution and numbers in the United States. Between 1989 and 2009, the number of states reporting the presence of introduced wild pigs went from 19 up to as many as 44. This increase, in part natural, but largely manmade, has caused an increased workload and cost for land and resource managers in areas where these new populations are found. This is the direct result of the damage that these introduced animals do. The cost of both these impacts and control efforts has been estimated to exceed a billion dollars annually (Pimentel 2007). The complexity of this problem has been further complicated by the widespread appeal and economic potential of these animals as a big game species (Tisdell 1982, Degner 1989). Wild pigs are a controversial problem that is not going away and will likely only get worse with time. Not only do they cause damage, but wild pigs are also survivors. They reproduce at a rate faster than any other mammal of comparable size, native or introduced; they can eat just about anything; and, they can live just about anywhere. On top of that, wild pigs are both very difficult to control and, with the possible exception of island ecosystems, almost impossible to eradicate (Dickson et al. 2001, Sweeney et al. 2003). The solution to the wild pig problem has not been readily apparent. The ultimate answer as to how to control these animals has not been found to date. In many ways, wild pigs are America's most successful large invasive species. All of which means that wild pigs are a veritable nightmare for land and resource managers trying to keep the numbers of these animals and the damage that they do under control. Since the more that one knows about an invasive species, the easier it is to deal with and hopefully control. For wild pigs then, it is better to 'know thy enemy' than to not, especially if one expects to be able to successfully control them. In an effort to better 'know thy enemy,' a two-day symposium was held in Augusta, Georgia, on April 21-22, 2004. This symposium was organized and sponsored by U.S.D.A. Forest Service-Savannah River (USFS-SR), U. S. Department of Energy-Savannah River Operations Office (DOE-SR), the Westinghouse Savannah River Company (WSRC), the South Carolina Chapter of the Soil & Water Conservation Society, and the Savannah River Ecology Laboratory (SREL). The goal of this symposium was to assemble researchers and land managers to first address various aspects of the biology and damage of wild pigs, and then review the control techniques and management of this invasive species. The result would then be a collected synopsis of what is known about wild pigs in the United States. Although the focus of the symposium was primarily directed toward federal agencies, presenters also included professionals from academic institutions, and private-sector control contractors and land managers. Most of the organizations associated with implementing this symposium were affiliated with the Savannah River Site (SRS), a 803 km{sup 2} federal nuclear facility, located in western South Carolina along the Savannah River. The SRS was a very appropriate facility to host this symposium. The SRS has been dealing with its wild pig problem since the early 1950s. A lot has been learned about these animals at the site over the ensuing decades. Between the USFS-SR, DOE-SR, SREL, and the site's management and operations contractor, which is currently Savannah River Nuclear Solutions, SRS organizations have conducted a wealth of research on this wild pig population, spanning a broad spectrum of topics and disciplines. In fact, the SRS wild pig population is among the most studied, and possibly is the best studied population of this invasive species found in the United States today. Unfortunately, with all of that work, the ultimate answer to controlling wild pigs and their impacts still has not been found. This volume represents the collected synopsis that was the goal of the aforementioned symposium.

  11. Airfoil structure

    DOE Patents [OSTI]

    Frey, G.A.; Twardochleb, C.Z.

    1998-01-13

    Past airfoil configurations have been used to improve aerodynamic performance and engine efficiencies. The present airfoil configuration further increases component life and reduces maintenance by reducing internal stress within the airfoil itself. The airfoil includes a chord and a span. Each of the chord and the span has a bow being summed to form a generally ``C`` configuration of the airfoil. The generally ``C`` configuration includes a compound bow in which internal stresses resulting from a thermal temperature gradient are reduced. The structural configuration reduces internal stresses resulting from thermal expansion. 6 figs.

  12. Airfoil structure

    DOE Patents [OSTI]

    Frey, Gary A.; Twardochleb, Christopher Z.

    1998-01-01

    Past airfoil configurations have been used to improve aerodynamic performance and engine efficiencies. The present airfoil configuration further increases component life and reduces maintenance by reducing internal stress within the airfoil itself. The airfoil includes a chord and a span. Each of the chord and the span has a bow being summed to form a generally "C" configuration of the airfoil. The generally "C" configuration includes a compound bow in which internal stresses resulting from a thermal temperature gradient are reduced. The structural configuration reduces internal stresses resulting from thermal expansion.

  13. Technoeconomic Boundary Analysis of Biological Pathways to Hydrogen Production

    Broader source: Energy.gov [DOE]

    Report documenting the biological and engineering characteristics of five algal and bacterial hydrogen production systems selected by DOE and NREL for evaluation.

  14. Methods for isolation and viability assessment of biological organisms

    DOE Patents [OSTI]

    Letant, Sonia Edith; Baker, Sarah Elyse; Bond, Tiziana; Chang, Allan Shih-Ping

    2015-02-03

    Isolation of biological or chemical organisms can be accomplished using a surface enhanced Raman scattering (SERS) system. The SERS system can be a single or a stacked plurality of photonic crystal membranes with noble-metal lined through pores for flowing analyte potentially containing the biological or chemical organisms. The through pores can be adapted to trap individual biological or chemical organisms and emit SERS spectra, which can then be detected by a detector and further analyzed for viability of the biological or chemical organism.

  15. Division of Chemical & Biological Sciences | The Ames Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Chemical & Biological Sciences Previous Pause Next Welcome Research teams in this Division ... We work to develop new catalysts that enable more efficient chemical reactions, discover ...

  16. Functionalized apertures for the detection of chemical and biological materials

    DOE Patents [OSTI]

    Letant, Sonia E.; van Buuren, Anthony W.; Terminello, Louis J.; Thelen, Michael P.; Hope-Weeks, Louisa J.; Hart, Bradley R.

    2010-12-14

    Disclosed are nanometer to micron scale functionalized apertures constructed on a substrate made of glass, carbon, semiconductors or polymeric materials that allow for the real time detection of biological materials or chemical moieties. Many apertures can exist on one substrate allowing for the simultaneous detection of numerous chemical and biological molecules. One embodiment features a macrocyclic ring attached to cross-linkers, wherein the macrocyclic ring has a biological or chemical probe extending through the aperture. Another embodiment achieves functionalization by attaching chemical or biological anchors directly to the walls of the apertures via cross-linkers.

  17. Molecular biology of signal transduction in plants. Abstracts

    SciTech Connect (OSTI)

    Not Available

    1991-12-31

    This volume contains abstracts of oral presentations and poster sessions of the 1991 Cold Springs Harbor Meeting entitled Molecular Biology of Signal Transduction in Plants.

  18. Biological and Environmental Research Advisory CommitteeOctober...

    Office of Science (SC) Website

    Sharlene Weatherwax .pdf file (440KB) - Office of Biological and Environmental Research Update Gary Geernaert .pdf file (2.1MB) - Climate and Environmental Sciences Division Update ...

  19. DOE Issues Request for Information on Biological Hydrogen Production...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    a request for information (RFI) seeking feedback from interested stakeholders regarding ... Feedback is specifically requested on the workshop report for the Biological Hydrogen ...

  20. Linqu Qinchi Biological Co Ltd | Open Energy Information

    Open Energy Info (EERE)

    City, Shandong Province, China Zip: 262600 Product: Shandong-based developer of a biogas plant that registered as a CDM project. References: Linqu Qinchi Biological Co.,...

  1. Synthetic biology for microbial production of lipid-based biofuels...

    Office of Scientific and Technical Information (OSTI)

    Synthetic biology for microbial production of lipid-based biofuels Citation Details In-Document Search This content will become publicly available on October 22, 2017 Title: ...

  2. Biological Conversion of Sugars to Hydrocarbons Technology Pathway...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    This technology pathway case investigates the biological conversion of biomass-derived sugars to hydrocarbon biofuels, utilizing data from recent literature references and ...

  3. Synthesis, crystal, and biological activity of a novel carbene...

    Office of Scientific and Technical Information (OSTI)

    carbene silver(I) complex with imidazole derivative Citation Details In-Document Search Title: Synthesis, crystal, and biological activity of a novel carbene silver(I) complex ...

  4. Importance of systems biology in engineering microbes for biofuel...

    Office of Scientific and Technical Information (OSTI)

    Importance of systems biology in engineering microbes for biofuel production Citation ... resources has catalyzed numerous research endeavors that focus on developing ...

  5. Systems biology analysis of Zymomonas mobilis ZM4 ethanol stress...

    Office of Scientific and Technical Information (OSTI)

    However, the molecular mechanisms of ethanol stress response have not been elucidated fully. In this study, ethanol stress responses were investigated using systems biology tools. ...

  6. DOE Issues Request for Information on Biological Hydrogen Production

    Broader source: Energy.gov [DOE]

    The Fuel Cell Technologies Office has issued a request for information seeking feedback from interested stakeholders regarding biological hydrogen production research and development.

  7. Technoeconomic Boundary Analysis of Biological Pathways to Hydrogen Production

    SciTech Connect (OSTI)

    James, B. D.; Baum, G. N.; Perez, J.; Baum, K. N.

    2009-09-01

    Report documenting the biological and engineering characteristics of five algal and bacterial hydrogen production systems selected by DOE and NREL for evaluation.

  8. Genomics and Systems Biology of Tuberculosis (2009 JGI User Meeting)

    ScienceCinema (OSTI)

    Galagan, James

    2011-04-25

    James Galagan from the Broad Institute spoke about the "Genomics and Systems Biology of TB" on March 26, 2009 during the 4th Annual User Meeting

  9. STRUCTURE-SOIL-STRUCTURE INTERACTION AT SRS | Department of Energy

    Office of Environmental Management (EM)

    STRUCTURE-SOIL-STRUCTURE INTERACTION AT SRS STRUCTURE-SOIL-STRUCTURE INTERACTION AT SRS Structure-Soil-Structure Interaction at SRS Structural Mechanics - SRS October 25, 2011 PDF...

  10. Enhancing Automatic Biological Pathway Generation with GO-based Gene Similarity

    SciTech Connect (OSTI)

    Sanfilippo, Antonio P.; Baddeley, Robert L.; Beagley, Nathaniel; Riensche, Roderick M.; Gopalan, Banu

    2009-08-03

    One of the greatest challenges in today’s analysis of microarray gene expression data is to identify pathways across regulated genes that underlie structural and functional changes of living cells in specific pathologies. Most current approaches to pathway generation are based on a reverse engineering approach in which pathway plausibility is solely induced from observed pathway data. These approaches tend to lack in generality as they are too dependent on the pathway observables from which they are induced. By contrast, alternative approaches that rely on prior biological knowledge may err in the opposite direction as the prior knowledge is usually not sufficiently tuned to the pathology of focus. In this paper, we present a novel pathway generation approach which combines insights from the reverse engineering and knowledge-based approaches to increase the biological plausibility and specificity of induced regulatory networks.

  11. Method and apparatus for biological sequence comparison

    DOE Patents [OSTI]

    Marr, T.G.; Chang, W.I.

    1997-12-23

    A method and apparatus are disclosed for comparing biological sequences from a known source of sequences, with a subject (query) sequence. The apparatus takes as input a set of target similarity levels (such as evolutionary distances in units of PAM), and finds all fragments of known sequences that are similar to the subject sequence at each target similarity level, and are long enough to be statistically significant. The invention device filters out fragments from the known sequences that are too short, or have a lower average similarity to the subject sequence than is required by each target similarity level. The subject sequence is then compared only to the remaining known sequences to find the best matches. The filtering member divides the subject sequence into overlapping blocks, each block being sufficiently large to contain a minimum-length alignment from a known sequence. For each block, the filter member compares the block with every possible short fragment in the known sequences and determines a best match for each comparison. The determined set of short fragment best matches for the block provide an upper threshold on alignment values. Regions of a certain length from the known sequences that have a mean alignment value upper threshold greater than a target unit score are concatenated to form a union. The current block is compared to the union and provides an indication of best local alignment with the subject sequence. 5 figs.

  12. Method and apparatus for biological sequence comparison

    DOE Patents [OSTI]

    Marr, Thomas G.; Chang, William I-Wei

    1997-01-01

    A method and apparatus for comparing biological sequences from a known source of sequences, with a subject (query) sequence. The apparatus takes as input a set of target similarity levels (such as evolutionary distances in units of PAM), and finds all fragments of known sequences that are similar to the subject sequence at each target similarity level, and are long enough to be statistically significant. The invention device filters out fragments from the known sequences that are too short, or have a lower average similarity to the subject sequence than is required by each target similarity level. The subject sequence is then compared only to the remaining known sequences to find the best matches. The filtering member divides the subject sequence into overlapping blocks, each block being sufficiently large to contain a minimum-length alignment from a known sequence. For each block, the filter member compares the block with every possible short fragment in the known sequences and determines a best match for each comparison. The determined set of short fragment best matches for the block provide an upper threshold on alignment values. Regions of a certain length from the known sequences that have a mean alignment value upper threshold greater than a target unit score are concatenated to form a union. The current block is compared to the union and provides an indication of best local alignment with the subject sequence.

  13. Solving the RNA polymerase I structural puzzle

    SciTech Connect (OSTI)

    Moreno-Morcillo, Mara; Taylor, Nicholas M. I.; Gruene, Tim; Legrand, Pierre; Rashid, Umar J.; Ruiz, Federico M.; Steuerwald, Ulrich; Mller, Christoph W.; Fernndez-Tornero, Carlos

    2014-10-01

    Details of the RNA polymerase I crystal structure determination provide a framework for solution of the structures of other multi-subunit complexes. Simple crystallographic experiments are described to extract relevant biological information such as the location of the enzyme active site. Knowing the structure of multi-subunit complexes is critical to understand basic cellular functions. However, when crystals of these complexes can be obtained they rarely diffract beyond 3 resolution, which complicates X-ray structure determination and refinement. The crystal structure of RNA polymerase I, an essential cellular machine that synthesizes the precursor of ribosomal RNA in the nucleolus of eukaryotic cells, has recently been solved. Here, the crucial steps that were undertaken to build the atomic model of this multi-subunit enzyme are reported, emphasizing how simple crystallographic experiments can be used to extract relevant biological information. In particular, this report discusses the combination of poor molecular replacement and experimental phases, the application of multi-crystal averaging and the use of anomalous scatterers as sequence markers to guide tracing and to locate the active site. The methods outlined here will likely serve as a reference for future structural determination of large complexes at low resolution.

  14. The OME Framework for genome-scale systems biology

    SciTech Connect (OSTI)

    Palsson, Bernhard O.; Ebrahim, Ali; Federowicz, Steve

    2014-12-19

    The life sciences are undergoing continuous and accelerating integration with computational and engineering sciences. The biology that many in the field have been trained on may be hardly recognizable in ten to twenty years. One of the major drivers for this transformation is the blistering pace of advancements in DNA sequencing and synthesis. These advances have resulted in unprecedented amounts of new data, information, and knowledge. Many software tools have been developed to deal with aspects of this transformation and each is sorely needed [1-3]. However, few of these tools have been forced to deal with the full complexity of genome-scale models along with high throughput genome- scale data. This particular situation represents a unique challenge, as it is simultaneously necessary to deal with the vast breadth of genome-scale models and the dizzying depth of high-throughput datasets. It has been observed time and again that as the pace of data generation continues to accelerate, the pace of analysis significantly lags behind [4]. It is also evident that, given the plethora of databases and software efforts [5-12], it is still a significant challenge to work with genome-scale metabolic models, let alone next-generation whole cell models [13-15]. We work at the forefront of model creation and systems scale data generation [16-18]. The OME Framework was borne out of a practical need to enable genome-scale modeling and data analysis under a unified framework to drive the next generation of genome-scale biological models. Here we present the OME Framework. It exists as a set of Python classes. However, we want to emphasize the importance of the underlying design as an addition to the discussions on specifications of a digital cell. A great deal of work and valuable progress has been made by a number of communities [13, 19-24] towards interchange formats and implementations designed to achieve similar goals. While many software tools exist for handling genome-scale metabolic models or for genome-scale data analysis, no implementations exist that explicitly handle data and models concurrently. The OME Framework structures data in a connected loop with models and the components those models are composed of. This results in the first full, practical implementation of a framework that can enable genome-scale design-build-test. Over the coming years many more software packages will be developed and tools will necessarily change. However, we hope that the underlying designs shared here can help to inform the design of future software.

  15. Armor structures

    DOE Patents [OSTI]

    Chu, Henry Shiu-Hung [Idaho Falls, ID; Lacy, Jeffrey M [Idaho Falls, ID

    2008-04-01

    An armor structure includes first and second layers individually containing a plurality of i-beams. Individual i-beams have a pair of longitudinal flanges interconnected by a longitudinal crosspiece and defining opposing longitudinal channels between the pair of flanges. The i-beams within individual of the first and second layers run parallel. The laterally outermost faces of the flanges of adjacent i-beams face one another. One of the longitudinal channels in each of the first and second layers faces one of the longitudinal channels in the other of the first and second layers. The channels of the first layer run parallel with the channels of the second layer. The flanges of the first and second layers overlap with the crosspieces of the other of the first and second layers, and portions of said flanges are received within the facing channels of the i-beams of the other of the first and second layers.

  16. Reversibly immobilized biological materials in monolayer films on electrodes

    DOE Patents [OSTI]

    Weaver, P.F.; Frank, A.J.

    1993-05-04

    Methods and techniques are described for reversibly binding charged biological particles in a fluid medium to an electrode surface. The methods are useful in a variety of applications. The biological materials may include microbes, proteins, and viruses. The electrode surface may consist of reversibly electroactive materials such as polyvinylferrocene, silicon-linked ferrocene or quinone.

  17. Reversibly immobilized biological materials in monolayer films on electrodes

    DOE Patents [OSTI]

    Weaver, Paul F. (Golden, CO); Frank, Arthur J. (Lakewood, CO)

    1993-01-01

    Methods and techniques are described for reversibly binding charged biological particles in a fluid medium to an electrode surface. The methods are useful in a variety of applications. The biological materials may include microbes, proteins, and viruses. The electrode surface may consist of reversibly electroactive materials such as polyvinylferrocene, silicon-linked ferrocene or quinone.

  18. Concentration and separation of biological organisms by ultrafiltration and

    Office of Scientific and Technical Information (OSTI)

    dielectrophoresis (Patent) | DOEPatents Concentration and separation of biological organisms by ultrafiltration and dielectrophoresis Title: Concentration and separation of biological organisms by ultrafiltration and dielectrophoresis Disclosed is a method for monitoring sources of public water supply for a variety of pathogens by using a combination of ultrafiltration techniques together dielectrophoretic separation techniques. Because water-borne pathogens, whether present due to

  19. Management of Biological Materials in Wastewater from Research & Development Facilities

    SciTech Connect (OSTI)

    Raney, Elizabeth A.; Moon, Thomas W.; Ballinger, Marcel Y.

    2011-04-01

    PNNL has developed and instituted a systematic approach to managing work with biological material that begins in the project planning phase and carries through implementation to waste disposal. This paper describes two major processes used at PNNL to analyze and mitigate the hazards associated with working with biological materials and evaluate them for disposal to the sewer, ground, or surface water in a manner that protects human health and the environment. The first of these processes is the Biological Work Permit which is used to identify requirements for handling, storing, and working with biological materials and the second is the Sewer Approval process which is used to evaluate discharges of wastewaters containing biological materials to assure they meet industrial wastewater permits and other environmental regulations and requirements.

  20. Synthesis, crystal structure, resistivity, and electronic structure...

    Office of Scientific and Technical Information (OSTI)

    Synthesis, crystal structure, resistivity, and electronic structure of the U(V) quaternary ... and electronic structure of the U(V) quaternary polyselenide Ba8PdU2Se12(Se2)2 ...

  1. Molecular biology of Homo sapiens: Abstracts of papers presented at the 51st Cold Spring Harbor symposium on quantitative biology

    SciTech Connect (OSTI)

    Watson, J.D.; Siniscalco, M.

    1986-01-01

    This volume contains abstracts of papers presented at the 51st Cold Springs Harbor Symposium on Quantitative Biology. The topic for this meeting was the ''Molecular Biology of Homo sapiens.'' Sessions were entitled Human Gene Map, Human Cancer Genes, Genetic Diagnosis, Human Evolution, Drugs Made Off Human Genes, Receptors, and Gene Therapy. (DT)

  2. Update on the Pfam5000 Strategy for Selection of Structural Genomics Targets

    Office of Scientific and Technical Information (OSTI)

    on the Pfam5000 Strategy for Selection of Structural Genomics Targets J.-M. Chandonia and S.E. Brenner Abstract- Structural Genomics is an international effort to determine the three-dimensional shapes of all important biological macromolecules, with a primary focus on proteins. Target proteins should be selected according to a strategy that is medically and biologically relevant, of good financial value, and tractable. In 2003, we presented the "Pfam5000" strategy, which involves

  3. Systems Biology Knowledgebase for a New Era in Biology A Genomics:GTL Report from the May 2008 Workshop

    SciTech Connect (OSTI)

    Gregurick, S.; Fredrickson, J. K.; Stevens, R.

    2009-03-01

    Biology has entered a systems-science era with the goal to establish a predictive understanding of the mechanisms of cellular function and the interactions of biological systems with their environment and with each other. Vast amounts of data on the composition, physiology, and function of complex biological systems and their natural environments are emerging from new analytical technologies. Effectively exploiting these data requires developing a new generation of capabilities for analyzing and managing the information. By revealing the core principles and processes conserved in collective genomes across all biology and by enabling insights into the interplay between an organism's genotype and its environment, systems biology will allow scientific breakthroughs in our ability to project behaviors of natural systems and to manipulate and engineer managed systems. These breakthroughs will benefit Department of Energy (DOE) missions in energy security, climate protection, and environmental remediation.

  4. Biologically based multistage modeling of radiation effects

    SciTech Connect (OSTI)

    William Hazelton; Suresh Moolgavkar; E. Georg Luebeck

    2005-08-30

    This past year we have made substantial progress in modeling the contribution of homeostatic regulation to low-dose radiation effects and carcinogenesis. We have worked to refine and apply our multistage carcinogenesis models to explicitly incorporate cell cycle states, simple and complex damage, checkpoint delay, slow and fast repair, differentiation, and apoptosis to study the effects of low-dose ionizing radiation in mouse intestinal crypts, as well as in other tissues. We have one paper accepted for publication in ''Advances in Space Research'', and another manuscript in preparation describing this work. I also wrote a chapter describing our combined cell-cycle and multistage carcinogenesis model that will be published in a book on stochastic carcinogenesis models edited by Wei-Yuan Tan. In addition, we organized and held a workshop on ''Biologically Based Modeling of Human Health Effects of Low dose Ionizing Radiation'', July 28-29, 2005 at Fred Hutchinson Cancer Research Center in Seattle, Washington. We had over 20 participants, including Mary Helen Barcellos-Hoff as keynote speaker, talks by most of the low-dose modelers in the DOE low-dose program, experimentalists including Les Redpath (and Mary Helen), Noelle Metting from DOE, and Tony Brooks. It appears that homeostatic regulation may be central to understanding low-dose radiation phenomena. The primary effects of ionizing radiation (IR) are cell killing, delayed cell cycling, and induction of mutations. However, homeostatic regulation causes cells that are killed or damaged by IR to eventually be replaced. Cells with an initiating mutation may have a replacement advantage, leading to clonal expansion of these initiated cells. Thus we have focused particularly on modeling effects that disturb homeostatic regulation as early steps in the carcinogenic process. There are two primary considerations that support our focus on homeostatic regulation. First, a number of epidemiologic studies using multistage carcinogenesis models that incorporate the ''initiation, promotion, and malignant conversion'' paradigm of carcinogenesis are indicating that promotion of initiated cells is the most important cellular mechanism driving the shape of the age specific hazard for many types of cancer. Second, we have realized that many of the genes that are modified in early stages of the carcinogenic process contribute to one or more of four general cellular pathways that confer a promotional advantage to cells when these pathways are disrupted.

  5. Structural Health Monitoring

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Structural Health Monitoring Engineering Institute Structural Health Monitoring Structural Health Monitoring is the process of implementing a damage detection strategy for...

  6. Stormwater Control Structures

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Stormwater Control Structures Stormwater Control Structures Stormwater control structures are engineered to control run-on and runoff water from suspected contaminated sites. ...

  7. Kinetic model for anaerobic digestion of biogas biological sludge

    SciTech Connect (OSTI)

    Pavlostathis, S.G.; Gossett, J.M.

    1986-10-01

    The principal objective of this study was the development and evaluation of a comprehensive kinetic model capable of predicting digester performance when fed biological sludge. Preliminary conversion mechanisms such as cell deaths, lysis, and hydrolysis responsible for rendering viable biological sludge organisms to available substrate were studied in depth. The results of this study indicate that hydrolysis of the dead, particulate biomass - primarily consisting of protein - is the slowest step, and therefore kinetically controls the overall process of an anaerobic digestion of biological sludge. A kinetic model was developed which could accurately describe digester performance and predict effluent quality.

  8. Arms Control: US and International efforts to ban biological weapons

    SciTech Connect (OSTI)

    Not Available

    1992-12-01

    The Bacteriological (Biological) and Toxin Weapons Convention, the treaty that bans the development, production, and stockpiling and acquisition of biological weapons was opened for signature in 1972 and came into force in 1975 after being ratified by 22 governments, including the depository nations of the USA, the United Kingdom, and the former Soviet Union. In support of the Convention, the USA later established export controls on items used to make biological weapons. Further, in accordance with the 1990 President`s Enhanced Proliferation Control Initiative, actions were taken to redefine and expand US export controls, as well as to encourage multilateral controls through the Australia Group. Thus far, the Convention has not been effective in stopping the development of biological weapons. The principal findings as to the reasons of the failures of the Convention are found to be: the Convention lacks universality, compliance measures are effective, advantage of verification may outweigh disadvantages. Recommendations for mitigating these failures are outlined in this report.

  9. Hydro Review: Computational Tools to Assess Turbine Biological Performance

    Broader source: Energy.gov [DOE]

    This review covers the BioPA method used to analyze the biological performance of proposed designs to help ensure the safety of fish passing through the turbines at the Priest Rapids Dam in Grant County, Washington.

  10. EA-2011: Proposed Release of Three Parasitoids for the Biological...

    Broader source: Energy.gov (indexed) [DOE]

    the continental U.S. for the biological control of the emerald ash borer, a nonnative invasive beetle. The DOE Oak Ridge Office reviewed the EA, adopted it, and issued a FONSI for...

  11. Methods of increasing secretion of polypeptides having biological activity

    DOE Patents [OSTI]

    Merino, Sandra

    2014-10-28

    The present invention relates to methods for producing a secreted polypeptide having biological activity, comprising: (a) transforming a fungal host cell with a fusion protein construct encoding a fusion protein, which comprises: (i) a first polynucleotide encoding a signal peptide; (ii) a second polynucleotide encoding at least a catalytic domain of an endoglucanase or a portion thereof; and (iii) a third polynucleotide encoding at least a catalytic domain of a polypeptide having biological activity; wherein the signal peptide and at least the catalytic domain of the endoglucanase increases secretion of the polypeptide having biological activity compared to the absence of at least the catalytic domain of the endoglucanase; (b) cultivating the transformed fungal host cell under conditions suitable for production of the fusion protein; and (c) recovering the fusion protein, a component thereof, or a combination thereof, having biological activity, from the cultivation medium.

  12. Methods of increasing secretion of polypeptides having biological activity

    DOE Patents [OSTI]

    Merino, Sandra

    2014-05-27

    The present invention relates to methods for producing a secreted polypeptide having biological activity, comprising: (a) transforming a fungal host cell with a fusion protein construct encoding a fusion protein, which comprises: (i) a first polynucleotide encoding a signal peptide; (ii) a second polynucleotide encoding at least a catalytic domain of an endoglucanase or a portion thereof; and (iii) a third polynucleotide encoding at least a catalytic domain of a polypeptide having biological activity; wherein the signal peptide and at least the catalytic domain of the endoglucanase increases secretion of the polypeptide having biological activity compared to the absence of at least the catalytic domain of the endoglucanase; (b) cultivating the transformed fungal host cell under conditions suitable for production of the fusion protein; and (c) recovering the fusion protein, a component thereof, or a combination thereof, having biological activity, from the cultivation medium.

  13. Methods of increasing secretion of polypeptides having biological activity

    DOE Patents [OSTI]

    Merino, Sandra

    2015-04-14

    The present invention relates to methods for producing a secreted polypeptide having biological activity, comprising: (a) transforming a fungal host cell with a fusion protein construct encoding a fusion protein, which comprises: (i) a first polynucleotide encoding a signal peptide; (ii) a second polynucleotide encoding at least a catalytic domain of an endoglucanase or a portion thereof; and (iii) a third polynucleotide encoding at least a catalytic domain of a polypeptide having biological activity; wherein the signal peptide and at least the catalytic domain of the endoglucanase increases secretion of the polypeptide having biological activity compared to the absence of at least the catalytic domain of the endoglucanase; (b) cultivating the transformed fungal host cell under conditions suitable for production of the fusion protein; and (c) recovering the fusion protein, a component thereof, or a combination thereof, having biological activity, from the cultivation medium.

  14. Methods of increasing secretion of polypeptides having biological activity

    DOE Patents [OSTI]

    Merino, Sandra

    2013-10-01

    The present invention relates to methods for producing a secreted polypeptide having biological activity, comprising: (a) transforming a fungal host cell with a fusion protein construct encoding a fusion protein, which comprises: (i) a first polynucleotide encoding a signal peptide; (ii) a second polynucleotide encoding at least a catalytic domain of an endoglucanase or a portion thereof; and (iii) a third polynucleotide encoding at least a catalytic domain of a polypeptide having biological activity; wherein the signal peptide and at least the catalytic domain of the endoglucanase increases secretion of the polypeptide having biological activity compared to the absence of at least the catalytic domain of the endoglucanase; (b) cultivating the transformed fungal host cell under conditions suitable for production of the fusion protein; and (c) recovering the fusion protein, a component thereof, or a combination thereof, having biological activity, from the cultivation medium.

  15. Biological Imaging by Soft X-Ray Diffraction Microscopy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Biological Imaging by Soft X-Ray Diffraction Microscopy Print Electron and x-ray microscopes are a valuable tool for both the life and materials sciences, but they are limited in...

  16. John Marohn > ProfessorChemistry and Chemical Biology > Faculty...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Marohn Professor Chemistry and Chemical Biology Research Group Webpage jam99@cornell.edu John Marohn earned a B.S. in Chemistry and a B.A. in Physics from the University of...

  17. Biology Chemistry & Material Science Laboratory 2 | Sample Preparation...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    2 Cynthia Patty | (650) 926-3925 Biology Chemistry & Material Science Laboratory 2 Inventory The BioChemMat Lab 2 (BCM 2) at SSRL is dedicated to researcher experiments, including...

  18. Biology Chemistry & Material Science Laboratory 1 | Sample Preparation...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    1 Cynthia Patty | (650) 926-3925 Biology Chemistry & Material Science Laboratory 1 Inventory The BioChemMat Lab 1 at SSRL is dedicated to researcher experiments, including x-ray...

  19. Investigations of biological processes in Austrian MBT plants

    SciTech Connect (OSTI)

    Tintner, J.; Smidt, E.; Boehm, K.; Binner, E.

    2010-10-15

    Mechanical biological treatment (MBT) of municipal solid waste (MSW) has become an important technology in waste management during the last decade. The paper compiles investigations of mechanical biological processes in Austrian MBT plants. Samples from all plants representing different stages of degradation were included in this study. The range of the relevant parameters characterizing the materials and their behavior, e.g. total organic carbon, total nitrogen, respiration activity and gas generation sum, was determined. The evolution of total carbon and nitrogen containing compounds was compared and related to process operation. The respiration activity decreases in most of the plants by about 90% of the initial values whereas the ammonium release is still ongoing at the end of the biological treatment. If the biogenic waste fraction is not separated, it favors humification in MBT materials that is not observed to such extent in MSW. The amount of organic carbon is about 15% dry matter at the end of the biological treatment.

  20. Biological assessments for the low energy demonstration accelerator, 1996

    SciTech Connect (OSTI)

    Cross, S.

    1997-03-01

    This report discusses the biological impact to the area around the Los Alamos National Laboratory of the Low Energy Demonstration Accelerator. In particular the impact to the soils, water quality, vegetation, and wildlife are discussed.

  1. September 2013 Most Viewed Documents for Biology And Medicine...

    Office of Scientific and Technical Information (OSTI)

    September 2013 Most Viewed Documents for Biology And Medicine Science Subject Feed Drug ... KRISHNA,C.R. (1999) 27 > Site-Directed Research and Development FY 2012 Annual Report , ...

  2. A comprehensive approach to decipher biological computation to achieve next

    Office of Scientific and Technical Information (OSTI)

    generation high-performance exascale computing. (Technical Report) | SciTech Connect A comprehensive approach to decipher biological computation to achieve next generation high-performance exascale computing. Citation Details In-Document Search Title: A comprehensive approach to decipher biological computation to achieve next generation high-performance exascale computing. The human brain (volume=1200cm3) consumes 20W and is capable of performing>10%5E16 operations/s. Current

  3. Importance of systems biology in engineering microbes for biofuel

    Office of Scientific and Technical Information (OSTI)

    production (Journal Article) | SciTech Connect Importance of systems biology in engineering microbes for biofuel production Citation Details In-Document Search Title: Importance of systems biology in engineering microbes for biofuel production Microorganisms have been rich sources for natural products, some of which have found use as fuels, commodity chemicals, specialty chemicals, polymers, and drugs, to name a few. The recent interest in production of transportation fuels from renewable

  4. Integration of Hyperspectral Imagery and Biosensors for Biological and

    Office of Scientific and Technical Information (OSTI)

    Chemical Facility Classification (Technical Report) | SciTech Connect Hyperspectral Imagery and Biosensors for Biological and Chemical Facility Classification Citation Details In-Document Search Title: Integration of Hyperspectral Imagery and Biosensors for Biological and Chemical Facility Classification Authors: Munsky, Brian [1] ; Yeager, John D. [1] ; Sellars, Scott [2] ; Mukundan, Harshini [1] ; Nadler, Brett Ross [1] + Show Author Affiliations Los Alamos National Laboratory UC Irvine

  5. Integration of Hyperspectral Imagery and Biosensors for Biological and

    Office of Scientific and Technical Information (OSTI)

    Chemical Facility Classification (Technical Report) | SciTech Connect Hyperspectral Imagery and Biosensors for Biological and Chemical Facility Classification Citation Details In-Document Search Title: Integration of Hyperspectral Imagery and Biosensors for Biological and Chemical Facility Classification × You are accessing a document from the Department of Energy's (DOE) SciTech Connect. This site is a product of DOE's Office of Scientific and Technical Information (OSTI) and is provided

  6. Integration of hyperspectral imagery and biosensors for biological and

    Office of Scientific and Technical Information (OSTI)

    chemical facility classification (Technical Report) | SciTech Connect hyperspectral imagery and biosensors for biological and chemical facility classification Citation Details In-Document Search Title: Integration of hyperspectral imagery and biosensors for biological and chemical facility classification Authors: Munsky, Brian [1] ; Sellars, Scott [2] ; Yeager, John D. [1] ; Mukundan, Harshini [1] ; Nadler, Brett Ross [1] + Show Author Affiliations Los Alamos National Laboratory UC Irvine

  7. Integration of hyperspectral imagery and biosensors for biological and

    Office of Scientific and Technical Information (OSTI)

    chemical facility classification (Technical Report) | SciTech Connect hyperspectral imagery and biosensors for biological and chemical facility classification Citation Details In-Document Search Title: Integration of hyperspectral imagery and biosensors for biological and chemical facility classification × You are accessing a document from the Department of Energy's (DOE) SciTech Connect. This site is a product of DOE's Office of Scientific and Technical Information (OSTI) and is provided

  8. Biological and Catalytic Conversion of Sugars and Lignin | Bioenergy | NREL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Biological and Catalytic Conversion of Sugars and Lignin Our research group, comprised of staff scientists, postdoctoral associates, students, and technicians, develops biological and chemical catalysts for cost-effective production of fuels and chemicals from terrestrial biomass. Two side-by-side images. The left figure illustrates the domains of the synthase enzyme, represented as "surfaces" or "blobs," embedded in a lipid bilayer, represented as yellow multi-jointed

  9. A Systems Biology Platform for Characterizing Regulatory and Metabolic

    Office of Scientific and Technical Information (OSTI)

    Pathways that Influence and Control Microbial Hydrogen Production (Technical Report) | SciTech Connect Technical Report: A Systems Biology Platform for Characterizing Regulatory and Metabolic Pathways that Influence and Control Microbial Hydrogen Production Citation Details In-Document Search Title: A Systems Biology Platform for Characterizing Regulatory and Metabolic Pathways that Influence and Control Microbial Hydrogen Production Authors: Collins, James J [1] + Show Author Affiliations

  10. A comprehensive approach to decipher biological computation to achieve next

    Office of Scientific and Technical Information (OSTI)

    generation high-performance exascale computing. (Technical Report) | SciTech Connect Technical Report: A comprehensive approach to decipher biological computation to achieve next generation high-performance exascale computing. Citation Details In-Document Search Title: A comprehensive approach to decipher biological computation to achieve next generation high-performance exascale computing. The human brain (volume=1200cm3) consumes 20W and is capable of performing>10%5E16 operations/s.

  11. Environmental Impacts of Wind Power Development on the Population Biology

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of Greater Prairie-Chickens | Department of Energy Impacts of Wind Power Development on the Population Biology of Greater Prairie-Chickens Environmental Impacts of Wind Power Development on the Population Biology of Greater Prairie-Chickens This report summarizes the results of a seven-year, DOE-funded research project, conducted by researchers from Kansas State University and the National Wind Coordinating Collaborative, to assess the effects of wind energy development in Kansas on the

  12. Ringleader: Jay Nix, Beamline Director for the Molecular Biology Consortium

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ringleader: Jay Nix, Beamline Director for the Molecular Biology Consortium Print Jay Nix started started the user program at Beamline 4.2.2 back in 2004, shortly after the Molecular Biology Consortium built the beamline. The macromolecular crystallography beamline is a little different than most at the ALS because it's privately managed by a consortium of 10 Midwest universities that pooled their money together to build the beamline, and now continue to do so to maintain it. Nix serves about 50

  13. Ringleader: Jay Nix, Beamline Director for the Molecular Biology Consortium

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Jay Nix, Beamline Director for the Molecular Biology Consortium Print Jay Nix started started the user program at Beamline 4.2.2 back in 2004, shortly after the Molecular Biology Consortium built the beamline. The macromolecular crystallography beamline is a little different than most at the ALS because it's privately managed by a consortium of 10 Midwest universities that pooled their money together to build the beamline, and now continue to do so to maintain it. Nix serves about 50 labs,

  14. Excitonic Quantum Random Walk in Biological Phycocyanin Nanowires |

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    MIT-Harvard Center for Excitonics Quantum Random Walk in Biological Phycocyanin Nanowires October 6, 2015 at 4:30pm/ 36-428 Yossi Paltiel Department of Applied Physics, The Hebrew University of Jerusalem yossi-3a The importance of quantum processes in biology is starting to be recognized. Quantum processes are being discussed in the context of enzyme function, olfaction, magnetic sensing and most prominently in photosynthetic light-harvesting complexes. These findings suggest that a key to

  15. Flow cytometry aids basic cell biology research and drug discovery

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Flow cytometry aids basic cell biology research and drug discovery Flow cytometry aids basic cell biology research and drug discovery Life Technologies Corporation and LANL have released the Attune® Acoustic Focusing Cytometer, featuring a reduced footprint, reduced consumables, and an affordable price. April 3, 2012 Attune® Acoustic Focusing Cytometer The Attune® Acoustic Focusing Cytometer achieves sample throughput at rates over 10 times faster than other cytometers-up to 1,000 μL per

  16. Comparative systems biology across an evolutionary gradient within the

    Office of Scientific and Technical Information (OSTI)

    Shewanella genus (Journal Article) | SciTech Connect Comparative systems biology across an evolutionary gradient within the Shewanella genus Citation Details In-Document Search Title: Comparative systems biology across an evolutionary gradient within the Shewanella genus To what extent genotypic differences translate to phenotypic variation remains a poorly understood issue of paramount importance for several cornerstone concepts of microbiology such as the species definition. Here, we take

  17. Copy of Synthetic Biology of Novel Thermophilic Bacteria for Enhanced

    Office of Scientific and Technical Information (OSTI)

    Production of Ethanol from 5-Carbon Sugars (LDRD %23 105944). (Conference) | SciTech Connect Copy of Synthetic Biology of Novel Thermophilic Bacteria for Enhanced Production of Ethanol from 5-Carbon Sugars (LDRD %23 105944). Citation Details In-Document Search Title: Copy of Synthetic Biology of Novel Thermophilic Bacteria for Enhanced Production of Ethanol from 5-Carbon Sugars (LDRD %23 105944). Abstract not provided. Authors: Reichmuth, David ; Kozina, Carol L. ; Sale, Kenneth L. ;

  18. New Microbiome Center to combine UChicago, Marine Biological Laboratory and

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Argonne expertise | Argonne National Laboratory Filamentous cyanobacteria from a tidal pond at Little Sippewissett salt marsh, Falmouth, Mass. Microbial communities - bacteria, viruses and fungi - affect every ecosystem on earth, including human bodies, oceans, our homes and the land around us, and are the focus of a new Microbiome Center jointly headed by Argonne, the Marine Biological Laboratory and the University of Chicago. (Image: S. Emil Ruff, Marine Biological Laboratory) Filamentous

  19. Large Scale Production Computing and Storage Requirements for Biological

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and Environmental Research: Target 2017 Large Scale Production Computing and Storage Requirements for Biological and Environmental Research: Target 2017 BERmontage.gif September 11-12, 2012 Hilton Rockville Hotel and Executive Meeting Center 1750 Rockville Pike Rockville, MD, 20852-1699 TEL: 1-301-468-1100 Sponsored by: U.S. Department of Energy Office of Science Office of Advanced Scientific Computing Research (ASCR) Office of Biological and Environmental Research (BER) National Energy

  20. Guidelines to improve airport preparedness against chemical and biological terrorism.

    SciTech Connect (OSTI)

    Edwards, Donna M.; Price, Phillip N.; Gordon, Susanna P.; Gadgil, Ashok

    2005-05-01

    Guidelines to Improve Airport Preparedness Against Chemical and Biological Terrorism is a 100-page document that makes concrete recommendations on improving security and assessing vulnerable areas and helps its readers understand the nature of chemical and biological attacks. The report has been turned over to Airports Council International (ACI) and the American Association of Airport Executives (AAAE), two organizations that together represent the interests of thousands of airport personnel and facilities in the U.S. and around the world.

  1. Computational Biology: A Recipe for Ligand-Binding Proteins

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Computational Biology: A Recipe for Ligand-Binding Proteins Authors: Ghirlanda, G. Title: Computational Biology: A Recipe for Ligand-Binding Proteins Source: Nature Year: 2013 Volume: 501 Pages: 177-178 ABSTRACT: Cellular cross-talk, enzymatic catalysis and regulation of gene expression all depend on molecular recognition. A method that allows the design of proteins with desired recognition sites could thus be revolutionary Date of online publication: Thu, 2013-09-12 Link online:

  2. Synthetic Biology of Novel Thermophilic Bacteria for Enhanced Production of

    Office of Scientific and Technical Information (OSTI)

    Ethanol from 5-Carbon Sugars (LDRD %23 105944). (Conference) | SciTech Connect Synthetic Biology of Novel Thermophilic Bacteria for Enhanced Production of Ethanol from 5-Carbon Sugars (LDRD %23 105944). Citation Details In-Document Search Title: Synthetic Biology of Novel Thermophilic Bacteria for Enhanced Production of Ethanol from 5-Carbon Sugars (LDRD %23 105944). Abstract not provided. Authors: Sapra, Rajat ; Reichmuth, David ; Kozina, Carol L. ; Sale, Kenneth L. ; Keasling, Jay ; Tang,

  3. Beamline 1.4.4

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Scientific disciplines: Biology, correlated electron systems, environmental science, geology, chemistry, polymers, soft materials GENERAL BEAMLINE INFORMATION...

  4. Beamline 1.4.3

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Scientific disciplines: Biology, correlated electron systems, environmental science, geology, chemistry, polymers, soft materials GENERAL BEAMLINE INFORMATION...

  5. Beamline 9.0.1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    disciplines: Applied science, biology, polymers, soft materials. Endstations: Serial crystallography Diffractive imaging Nanosurveyor GENERAL BEAMLINE INFORMATION...

  6. BIOISIS: Biological Macromolecules by Small Angle X-ray Scattering (SAXS)

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Tainer, John [Scripps Research Institute; Hura, Greg [LBNL; Rambo, Robert P. [LBNL

    BIOISIS is an open access database dedicated to the study of biological macromolecules by small angle X-ray scattering (SAXS). BIOISIS aims to become the complete source for the deposition, distribution and maintenance of small angle X-ray scattering data and technologies. The database is designed around the concept of an experiment and relates a specific experiment to a set of genes, organisms, computational models and experimental data. As of May 2012, BIOSIS contains 7,118 genes covering four different organisms. Forty-two modeled structures are available. Clicking on a structures reveals scattering curves, experimental conditions, and experimental values. The data are collected at Beamline 12.3.1 of the Advanced Light Source (ALS).[Copied with editing from http://www.bioisis.net/about

  7. Behavior of nanoceria in biologically-relevant environments

    SciTech Connect (OSTI)

    Kumar, Amit; Das, Soumen; Munusamy, Prabhakaran; Self, William; Baer, Donald R.; Sayle, Dean C.; Seal, Sudipta

    2014-09-08

    Cerium oxide nanoparticles (CNPs) have gained a considerable attention in biological research due to their anti-oxidant like behaviour and regenerative nature. The current literature on CNPs reports many successful attempts on harnessing the beneficial therapeutic properties in biology. However studies have also shown toxicity effect with some types of CNPs. This review discusses issues associated with the behaviours of CNPs in biological systems and identifies key knowledge gaps. We explore how salient physicochemical properties (size, surface chemistry, surface stabilizers) contribute to the potential positive and negative aspects of nanoceria in biological systems. Based on variations of results reported in the literature, important issues need to be addressed. Are we really studying the same particles with slight variations in size and physicochemical properties or do the particles being examined have fundamentally different behaviours? Are the variations observed in the result of differences in the initial properties of the particles or the results of downstream effects that emerge as the particles are prepared for specific studies and they interact with biological or other environmental moieties? How should particles be appropriately prepared for relevant environmental/toxicology/safety studies? It is useful to recognize that nanoparticles encompass some of the same complexities and variability associated with biological components

  8. Borrowing from Nature to Produce Highly Structured Biomimetic Materials

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Borrowing from Nature to Produce Highly Structured Biomimetic Materials Print Doing Nature One Better Biological tissues such as those that make up our organs are amazing feats of structural engineering. Even more fantastic, the tissues assemble themselves from building blocks based on the proteins that cells synthesize without any outside direction. And, if this isn't mind-boggling enough, consider how all this takes place over large distances with both precision and accuracy. Chemists and

  9. Borrowing from Nature to Produce Highly Structured Biomimetic Materials

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Borrowing from Nature to Produce Highly Structured Biomimetic Materials Print Doing Nature One Better Biological tissues such as those that make up our organs are amazing feats of structural engineering. Even more fantastic, the tissues assemble themselves from building blocks based on the proteins that cells synthesize without any outside direction. And, if this isn't mind-boggling enough, consider how all this takes place over large distances with both precision and accuracy. Chemists and

  10. Borrowing from Nature to Produce Highly Structured Biomimetic Materials

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Borrowing from Nature to Produce Highly Structured Biomimetic Materials Print Doing Nature One Better Biological tissues such as those that make up our organs are amazing feats of structural engineering. Even more fantastic, the tissues assemble themselves from building blocks based on the proteins that cells synthesize without any outside direction. And, if this isn't mind-boggling enough, consider how all this takes place over large distances with both precision and accuracy. Chemists and

  11. Borrowing from Nature to Produce Highly Structured Biomimetic Materials

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Borrowing from Nature to Produce Highly Structured Biomimetic Materials Print Doing Nature One Better Biological tissues such as those that make up our organs are amazing feats of structural engineering. Even more fantastic, the tissues assemble themselves from building blocks based on the proteins that cells synthesize without any outside direction. And, if this isn't mind-boggling enough, consider how all this takes place over large distances with both precision and accuracy. Chemists and

  12. Borrowing from Nature to Produce Highly Structured Biomimetic Materials

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Borrowing from Nature to Produce Highly Structured Biomimetic Materials Print Doing Nature One Better Biological tissues such as those that make up our organs are amazing feats of structural engineering. Even more fantastic, the tissues assemble themselves from building blocks based on the proteins that cells synthesize without any outside direction. And, if this isn't mind-boggling enough, consider how all this takes place over large distances with both precision and accuracy. Chemists and

  13. Achievements of structural genomics (Conference) | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    Conference: Achievements of structural genomics Citation Details In-Document Search Title: Achievements of structural genomics Authors: Terwilliger, Thomas C [1] + Show Author Affiliations Los Alamos National Laboratory Publication Date: 2012-03-08 OSTI Identifier: 1170663 Report Number(s): LA-UR-12-01239; LA-UR-12-1239 DOE Contract Number: AC52-06NA25396 Resource Type: Conference Resource Relation: Conference: Institute of biological chemistry, academia sinica Taiwan ; March 21, 2012 ; Taipai,

  14. Borrowing from Nature to Produce Highly Structured Biomimetic Materials

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Borrowing from Nature to Produce Highly Structured Biomimetic Materials Print Doing Nature One Better Biological tissues such as those that make up our organs are amazing feats of structural engineering. Even more fantastic, the tissues assemble themselves from building blocks based on the proteins that cells synthesize without any outside direction. And, if this isn't mind-boggling enough, consider how all this takes place over large distances with both precision and accuracy. Chemists and

  15. Target Selection and Deselection at the Berkeley Structural Genomics Center

    Office of Scientific and Technical Information (OSTI)

    Target Selection and Deselection at the Berkeley Structural Genomics Center 45-letter short title: Target Selection and Deselection at the BSGC Authors: John-Marc Chandonia 1 , Sung-Hou Kim 1,2 , and Steven E. Brenner 1,3 Address for correspondence: Steven E. Brenner Department of Plant and Microbial Biology 461A Koshland Hall University of California Berkeley, CA 94720-3102 email: brenner@compbio.berkeley.edu fax: (413) 280-7813 Affiliations: 1 - Berkeley Structural Genomics Center, Physical

  16. Developing Enzyme and Biomimetic Catalysts for Upgrading Heavy Crudes via Biological Hydrogenation and Hydrodesulfurization

    SciTech Connect (OSTI)

    Borole, A P

    2006-08-22

    The recovery and conversion of heavy oils is limited due to the high viscosity of these crudes and their high heteroatom content. Conventional technology relies on thermochemical hydrogenation and hydrodesulfurization to address these problems and is energy intensive due to the high operating temperature and pressure. This project was initiated to explore biological catalysts for adding hydrogen to the heavy oil molecules. Biological enzymes are efficient at hydrogen splitting at very mild conditions such as room temperature and pressure, however, they are very specific in terms of the substrates they hydrogenate. The goal of the project was to investigate how the specificity of these enzymes can be altered to develop catalysts for oil upgrading. Three approaches were used. First was to perform chemical modification of the enzyme surface to improve binding of other non-natural substrates. Second approach was to expose the deeply buried catalytic active site of the enzyme by removal of protein scaffolding to enable better interaction with other substrates. The third approach was based on molecular biology to develop genetically engineered systems for enabling targeted structural changes in the enzyme. The first approach was found to be limited in success due to the non-specificity of the chemical modification and inability to target the region near the active site or the site of substrate binding. The second approach produced a smaller catalyst capable of catalyzing hydrogen splitting, however, further experimentation is needed to address reproducibility and stability issues. The third approach which targeted cloning of hydrogenase in alternate hosts demonstrated progress, although further work is necessary to complete the cloning process. The complex nature of the hydrogenase enzyme structure-function relationship and role of various ligands in the protein require significant more research to better understand the enzyme and to enable success in strategies in developing catalysts with broader specificity as that required for crude upgrading.

  17. Programmed assembly of nanoscale structures using peptoids.

    SciTech Connect (OSTI)

    Ren, Jianhua; Russell, Scott; Morishetti, Kiran; Robinson, David B.; Zuckermann, Ronald N.; Buffleben, George M.; Hjelm, Rex P.; Kent, Michael Stuart

    2011-02-01

    Sequence-specific polymers are the basis of the most promising approaches to bottom-up programmed assembly of nanoscale materials. Examples include artificial peptides and nucleic acids. Another class is oligo(N-functional glycine)s, also known as peptoids, which permit greater sidegroup diversity and conformational control, and can be easier to synthesize and purify. We have developed a set of peptoids that can be used to make inorganic nanoparticles more compatible with biological sequence-specific polymers so that they can be incorporated into nucleic acid or other biologically based nanostructures. Peptoids offer degrees of modularity, versatility, and predictability that equal or exceed other sequence-specific polymers, allowing for rational design of oligomers for a specific purpose. This degree of control will be essential to the development of arbitrarily designed nanoscale structures.

  18. Tobacco mosaic virus: A biological building block for micro/nano...

    Office of Scientific and Technical Information (OSTI)

    Tobacco mosaic virus: A biological building block for micronanobio systems Citation Details In-Document Search Title: Tobacco mosaic virus: A biological building block for micro...

  19. Sage-Grouse and Wind Energy: Biology, Habits, and Potential Effects...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Sage-Grouse and Wind Energy: Biology, Habits, and Potential Effects from Development Sage-Grouse and Wind Energy: Biology, Habits, and Potential Effects from Development ...

  20. Culture, and a Metrics Methodology for Biological Countermeasure Scenarios

    SciTech Connect (OSTI)

    Simpson, Mary J.

    2007-03-15

    Outcome Metrics Methodology defines a way to evaluate outcome metrics associated with scenario analyses related to biological countermeasures. Previous work developed a schema to allow evaluation of common elements of impacts across a wide range of potential threats and scenarios. Classes of metrics were identified that could be used by decision makers to differentiate the common bases among disparate scenarios. Typical impact metrics used in risk calculations include the anticipated number of deaths, casualties, and the direct economic costs should a given event occur. There are less obvious metrics that are often as important and require more intensive initial work to be incorporated. This study defines a methodology for quantifying, evaluating, and ranking metrics other than direct health and economic impacts. As has been observed with the consequences of Hurricane Katrina, impacts to the culture of specific sectors of society are less obvious on an immediate basis but equally important over the ensuing and long term. Culture is used as the example class of metrics within which • requirements for a methodology are explored • likely methodologies are examined • underlying assumptions for the respective methodologies are discussed • the basis for recommending a specific methodology is demonstrated. Culture, as a class of metrics, is shown to consist of political, sociological, and psychological elements that are highly valued by decision makers. In addition, cultural practices, dimensions, and kinds of knowledge offer complementary sets of information that contribute to the context within which experts can provide input. The quantification and evaluation of sociopolitical, socio-economic, and sociotechnical impacts depend predominantly on subjective, expert judgment. Epidemiological data is limited, resulting in samples with statistical limits. Dose response assessments and curves depend on the quality of data and its relevance to human modes of exposure. With uncertain data and limited common units, the aggregation of results is not inherently obvious. Candidate methodologies discussed include statistical, analytical, and expert-based numerical approaches. Most statistical methods require large amounts of data with a random distribution of values for validity. Analytical methods predominate wherein structured data or patterns are evident and randomness is low. The analytical hierarchy process is shown to satisfy all requirements and provide a detailed method for measurement that depends on expert judgment by decision makers.

  1. Cell-based composite materials with programmed structures and functions

    DOE Patents [OSTI]

    None

    2016-03-01

    The present invention is directed to the use of silicic acid to transform biological materials, including cellular architecture into inorganic materials to provide biocomposites (nanomaterials) with stabilized structure and function. In the present invention, there has been discovered a means to stabilize the structure and function of biological materials, including cells, biomolecules, peptides, proteins (especially including enzymes), lipids, lipid vesicles, polysaccharides, cytoskeletal filaments, tissue and organs with silicic acid such that these materials may be used as biocomposites. In many instances, these materials retain their original biological activity and may be used in harsh conditions which would otherwise destroy the integrity of the biological material. In certain instances, these biomaterials may be storage stable for long periods of time and reconstituted after storage to return the biological material back to its original form. In addition, by exposing an entire cell to form CSCs, the CSCs may function to provide a unique system to study enzymes or a cascade of enzymes which are otherwise unavailable.

  2. Solid-water detoxifying reagents for chemical and biological agents

    DOE Patents [OSTI]

    Hoffman, Dennis M.; Chiu, Ing Lap

    2006-04-18

    Formation of solid-water detoxifying reagents for chemical and biological agents. Solutions of detoxifying reagent for chemical and biological agents are coated using small quantities of hydrophobic nanoparticles by vigorous agitation or by aerosolization of the solution in the presence of the hydrophobic nanoparticles to form a solid powder. For example, when hydrophobic fumed silica particles are shaken in the presence of IN oxone solution in approximately a 95:5-weight ratio, a dry powder results. The hydrophobic silica forms a porous coating of insoluble fine particles around the solution. Since the chemical or biological agent tends to be hydrophobic on contact with the weakly encapsulated detoxifying solution, the porous coating breaks down and the detoxifying reagent is delivered directly to the chemical or biological agent for maximum concentration at the point of need. The solid-water (coated) detoxifying solutions can be blown into contaminated ventilation ducting or other difficult to reach sites for detoxification of pools of chemical or biological agent. Once the agent has been detoxified, it can be removed by flushing the area with air or other techniques.

  3. Request for Travel Funds for Systems Radiation Biology Workshop

    SciTech Connect (OSTI)

    Barcellos-Hoff, Mary Helen

    2014-03-22

    The 3rd International Systems Radiation Biology Workshop brought together the major European, US and Japanese research programs on radiation risk as well as selected experts representing systems biological approaches to discuss how the new methodologies could be best exploited for low dose research. A significant part of the workshop was devoted to discussions organised as breakout group sessions. To facilitate discussions number of participants was limited to 60 persons. To achieve the goals of this symposium in this international conference, support from DOE is vital. Hence, this proposal requested support in the amount of $15,000 to cover the travel expenses of international experts and radiation biology scientists from the United States. This supporting mechanism was clearly identified to the selected US participants as a conference support award from the DOE (See attached PDF). The workshop was an outstanding opportunity to strengthen interactions between leading experts in the emerging areas of radiation sciences, and will also provide opportunities for younger scientists to meet with experts and discuss their results. This workshop was designed to endorse active engagement in international collaboration. A major objective of this conference was to effectively communicate research results, in order to ensure that current thinking reflects sound science of radiation biology. Further, this international event addressed the use and success of scientific initiatives in radiation biology for policymakers, standard-setters, and the general public.

  4. Decontamination of biological warfare agents by a microwave plasma torch

    SciTech Connect (OSTI)

    Lai, Wilson; Lai, Henry; Kuo, Spencer P.; Tarasenko, Olga; Levon, Kalle

    2005-02-01

    A portable arc-seeded microwave plasma torch running stably with airflow is described and applied for the decontamination of biological warfare agents. Emission spectroscopy of the plasma torch indicated that this torch produced an abundance of reactive atomic oxygen that could effectively oxidize biological agents. Bacillus cereus was chosen as a simulant of Bacillus anthracis spores for biological agent in the decontamination experiments. Decontamination was performed with the airflow rate of 0.393 l/s, corresponding to a maximum concentration of atomic oxygen produced by the torch. The experimental results showed that all spores were killed in less than 8 s at 3 cm distance, 12 s at 4 cm distance, and 16 s at 5 cm distance away from the nozzle of the torch.

  5. Formulations for neutralization of chemical and biological toxants

    DOE Patents [OSTI]

    Tadros, Maher E.; Tucker, Mark D.

    2003-05-20

    A formulation and method of making that neutralizes the adverse health effects of both chemical and biological compounds, especially chemical warfare (CW) and biological warfare (BW) agents. The formulation of the present invention non-toxic and non-corrosive and can be delivered by a variety of means and in different phases. The formulation provides solubilizing compounds that serve to effectively render the chemical and biological compounds, particularly CW and BW compounds, susceptible to attack and at least one reactive compound that serves to attack (and detoxify or kill) the compound. The at least one reactive compound can be an oxidizing compound, a nucleophilic compound or a mixture of both. The formulation can kill up to 99.99999% of bacterial spores within one hour of exposure.

  6. Enhanced formulations for neutralization of chemical, biological and industrial toxants

    DOE Patents [OSTI]

    Tucker, Mark D. [Albuqueque, NM

    2008-06-24

    An enhanced formulation and method of making that neutralizes the adverse health effects of both chemical and biological compounds, especially chemical warfare (CW) and biological warfare (BW) agents, and toxic industrial chemicals. The enhanced formulation according to the present invention is non-toxic and non-corrosive and can be delivered by a variety of means and in different phases. The formulation provides solubilizing compounds that serve to effectively render the chemical and biological compounds, particularly CW and BW compounds, susceptible to attack, and at least one reactive compound that serves to attack (and detoxify or kill) the compound. The formulation includes at least one solubilizing agent, a reactive compound, a bleaching activator and water.

  7. Method and apparatus for determining nutrient stimulation of biological processes

    DOE Patents [OSTI]

    Colwell, Frederick S.; Geesey, Gill G.; Gillis, Richard J.; Lehman, R. Michael

    1999-01-01

    A method and apparatus for determining the nutrients to stimulate microorganisms in a particular environment. A representative sample of microorganisms from a particular environment are contacted with multiple support means wherein each support means has intimately associated with the surface of the support means a different nutrient composition for said microorganisms in said sample. The multiple support means is allowed to remain in contact with the microorganisms in the sample for a time period sufficient to measure difference in microorganism effects for the multiple support means. Microorganism effects for the multiple support means are then measured and compared. The invention is particularly adaptable to being conducted in situ. The additional steps of regulating nutrients added to the particular environment of microorganisms can enhance the desired results. Biological systems particularly suitable for this invention are bioremediation, biologically enhanced oil recovery, biological leaching of metals, and agricultural bioprocesses.

  8. Method and apparatus for determining nutrient stimulation of biological processes

    DOE Patents [OSTI]

    Colwell, Frederick S.; Geesey, Gill G.; Gillis, Richard J.; Lehman, R. Michael

    1997-01-01

    A method and apparatus for determining the nutrients to stimulate microorganisms in a particular environment. A representative sample of microorganisms from a particular environment are contacted with multiple support means wherein each support means has intimately associated with the surface of the support means a different nutrient composition for said microorganisms in said sample. The multiple support means is allowed to remain in contact with the microorganisms in the sample for a time period sufficient to measure differences in microorganism effects for the multiple support means. Microorganism effects for the multiple support means are then measured and compared. The invention is particularly adaptable to being conducted in situ. The additional steps of regulating nutrients added to the particular environment of microorganisms can enhance the desired results. Biological systems particularly suitable for this invention are bioremediation, biologically enhanced oil recovery, biological leaching of metals, and agricultural bioprocesses.

  9. Method and apparatus for determining nutrient stimulation of biological processes

    DOE Patents [OSTI]

    Colwell, F.S.; Geesey, G.G.; Gillis, R.J.; Lehman, R.M.

    1997-11-11

    A method and apparatus is described for determining the nutrients to stimulate microorganisms in a particular environment. A representative sample of microorganisms from a particular environment are contacted with multiple support means wherein each support means has intimately associated with the surface of the support means a different nutrient composition for said microorganisms in said sample. The multiple support means is allowed to remain in contact with the microorganisms in the sample for a time period sufficient to measure differences in microorganism effects for the multiple support means. Microorganism effects for the multiple support means are then measured and compared. The invention is particularly adaptable to being conducted in situ. The additional steps of regulating nutrients added to the particular environment of microorganisms can enhance the desired results. Biological systems particularly suitable for this invention are bioremediation, biologically enhanced oil recovery, biological leaching of metals, and agricultural bioprocesses. 5 figs.

  10. Method and apparatus for determining nutrient stimulation of biological processes

    DOE Patents [OSTI]

    Colwell, F.S.; Geesey, G.G.; Gillis, R.J.; Lehman, R.M.

    1999-07-13

    A method and apparatus are disclosed for determining the nutrients to stimulate microorganisms in a particular environment. A representative sample of microorganisms from a particular environment are contacted with multiple support means wherein each support means has intimately associated with the surface of the support means a different nutrient composition for microorganisms in the sample. The multiple support means is allowed to remain in contact with the microorganisms in the sample for a time period sufficient to measure difference in microorganism effects for the multiple support means. Microorganism effects for the multiple support means are then measured and compared. The invention is particularly adaptable to being conducted in situ. The additional steps of regulating nutrients added to the particular environment of microorganisms can enhance the desired results. Biological systems particularly suitable for this invention are bioremediation, biologically enhanced oil recovery, biological leaching of metals, and agricultural bioprocesses. 5 figs.

  11. Renewable Energy from Synthetic Biology (LBNL Science at the Theater)

    ScienceCinema (OSTI)

    Keasling, Jay

    2011-04-28

    Jay Keasling, co-leader of Berkeley Lab's Helios Project, is a groundbreaking researcher in the new scientific field of synthetic biology. In Helios, he directs the biology program, incorporating a range of approaches to increasing the efficacy and economy of plants and cellulose-degrading microbes to make solar-based fuels. He is a UC Berkeley professor of Chemical and Bioengineering, and founder of Amyris Biotechnologies, a company that was honored as a Technology Pioneer for 2006 by the World Economic Forum. Keasling has succeeded in using synthetic biology to develop a yeast-based production scheme for precursors of the antimalarial drug artemisinin in work funded by the Bill & Melinda Gates Foundation.

  12. Chemical and biological nonproliferation program. FY99 annual report

    SciTech Connect (OSTI)

    2000-03-01

    This document is the first of what will become an annual report documenting the progress made by the Chemical and Biological Nonproliferation Program (CBNP). It is intended to be a summary of the program's activities that will be of interest to both policy and technical audiences. This report and the annual CBNP Summer Review Meeting are important vehicles for communication with the broader chemical and biological defense and nonproliferation communities. The Chemical and Biological Nonproliferation Program Strategic Plan is also available and provides additional detail on the program's context and goals. The body of the report consists of an overview of the program's philosophy, goals and recent progress in the major program areas. In addition, an appendix is provided with more detailed project summaries that will be of interest to the technical community.

  13. Semiconductor Quantum Rods as Single Molecule FluorescentBiological Labels

    SciTech Connect (OSTI)

    Fu, Aihua; Gu, Weiwei; Boussert, Benjamine; Koski, Kristie; Gerion, Daniele; Manna, Liberato; Le Gros, Mark; Larabell, Carolyn; Alivisatos, A. Paul

    2006-05-29

    In recent years, semiconductor quantum dots have beenapplied with great advantage in a wide range of biological imagingapplications. The continuing developments in the synthesis of nanoscalematerials and specifically in the area of colloidal semiconductornanocrystals have created an opportunity to generate a next generation ofbiological labels with complementary or in some cases enhanced propertiescompared to colloidal quantum dots. In this paper, we report thedevelopment of rod shaped semiconductor nanocrystals (quantum rods) asnew fluorescent biological labels. We have engineered biocompatiblequantum rods by surface silanization and have applied them fornon-specific cell tracking as well as specific cellular targeting. Theproperties of quantum rods as demonstrated here are enhanced sensitivityand greater resistance for degradation as compared to quantum dots.Quantum rods have many potential applications as biological labels insituations where their properties offer advantages over quantumdots.

  14. Variably porous structures

    DOE Patents [OSTI]

    Braun, Paul V.; Yu, Xindi

    2011-01-18

    A method of making a monolithic porous structure, comprises electrodepositing a material on a template; removing the template from the material to form a monolithic porous structure comprising the material; and electropolishing the monolithic porous structure.

  15. Synchrotron IR microspectroscopy for protein structure analysis: Potential and questions

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Yu, Peiqiang

    2006-01-01

    Synchrotron radiation-based Fourier transform infrared microspectroscopy (S-FTIR) has been developed as a rapid, direct, non-destructive, bioanalytical technique. This technique takes advantage of synchrotron light brightness and small effective source size and is capable of exploring the molecular chemical make-up within microstructures of a biological tissue without destruction of inherent structures at ultra-spatial resolutions within cellular dimension. To date there has been very little application of this advanced technique to the study of pure protein inherent structure at a cellular level in biological tissues. In this review, a novel approach was introduced to show the potential of the newly developed, advancedmore » synchrotron-based analytical technology, which can be used to localize relatively “pure“ protein in the plant tissues and relatively reveal protein inherent structure and protein molecular chemical make-up within intact tissue at cellular and subcellular levels. Several complex protein IR spectra data analytical techniques (Gaussian and Lorentzian multi-component peak modeling, univariate and multivariate analysis, principal component analysis (PCA), and hierarchical cluster analysis (CLA) are employed to relatively reveal features of protein inherent structure and distinguish protein inherent structure differences between varieties/species and treatments in plant tissues. By using a multi-peak modeling procedure, RELATIVE estimates (but not EXACT determinations) for protein secondary structure analysis can be made for comparison purpose. The issues of pro- and anti-multi-peaking modeling/fitting procedure for relative estimation of protein structure were discussed. By using the PCA and CLA analyses, the plant molecular structure can be qualitatively separate one group from another, statistically, even though the spectral assignments are not known. The synchrotron-based technology provides a new approach for protein structure research in biological tissues at ultraspatial resolutions.« less

  16. Air monitoring and detection of chemical and biological agents

    SciTech Connect (OSTI)

    Leonelli, J.; Althouse, M.L.

    1999-06-01

    This volume contains the proceedings of SPIE`s remote sensing symposium which was held November 2--3, 1998 in Boston, Massachusetts. Topics of discussion include the following: system simulations, atmospheric modeling, and performance prediction studies of chemical warfare remote sensing technologies; ultraviolet laser-induced fluorescence and aerosol detection methods for remote sensing of biological warfare agents; passive detection methods for remote detection of chemical warfare agents; and lidar-based system performance assessments, demonstrations, and new concepts for chemical warfare/biological warfare detection.

  17. Concentrated formulations and methods for neutralizing chemical and biological toxants

    SciTech Connect (OSTI)

    Tucker, Mark D.; Betty, Rita G.; Tadros, Maher E.

    2004-04-20

    A formulation and method of making and using that neutralizes the adverse health effects of both chemical and biological toxants, especially chemical warfare (CW) and biological warfare (BW) agents. The aqueous formulation is non-toxic and non-corrosive and can be delivered as a long-lasting foam, spray, or fog. The formulation includes solubilizing compounds that serve to effectively render the CW or BW toxant susceptible to attack, so that a nucleophillic agent can attack the compound via a hydrolysis or oxidation reaction. The formulation can kill up to 99.99999% of bacterial spores within one hour of exposure.

  18. Biological Imaging by Soft X-Ray Diffraction Microscopy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Biological Imaging by Soft X-Ray Diffraction Microscopy Biological Imaging by Soft X-Ray Diffraction Microscopy Print Wednesday, 30 November 2005 00:00 Electron and x-ray microscopes are a valuable tool for both the life and materials sciences, but they are limited in their ability to image with nanometer-scale resolution in three dimensions nonperiodic objects that are several microns in size. To fill this gap, the technique of coherent x-ray diffraction imaging now under development takes

  19. Computational Proteomics: High-throughput Analysis for Systems Biology

    SciTech Connect (OSTI)

    Cannon, William R.; Webb-Robertson, Bobbie-Jo M.

    2007-01-03

    High-throughput (HTP) proteomics is a rapidly developing field that offers the global profiling of proteins from a biological system. The HTP technological advances are fueling a revolution in biology, enabling analyses at the scales of entire systems (e.g., whole cells, tumors, or environmental communities). However, simply identifying the proteins in a cell is insufficient for understanding the underlying complexity and operating mechanisms of the overall system. Systems level investigations are relying more and more on computational analyses, especially in the field of proteomics generating large-scale global data.

  20. COLLOQUIUM: Plasma Mediated Effects on Biological Cells | Princeton Plasma

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Physics Lab October 8, 2014, 4:00pm to 5:30pm Colloquia MBG Auditorium COLLOQUIUM: Plasma Mediated Effects on Biological Cells Professor Mounir Laroussi Old Dominion University Low temperature plasma (LTP) in air-containing gas mixtures produce reactive oxygen species (ROS) such as O, O2-, and OH and reactive nitrogen species (RNS) such as NO and NO2 which exhibit strong oxidative properties and/or trigger signaling pathways in biological cells. For example oxidation of the lipids and

  1. Detection and differentiation of coxiella burnetii in biological fluids

    DOE Patents [OSTI]

    Frazier, Marvin E.; Mallavia, Louis P.; Samuel, James E.; Baca, Oswald G.

    1990-01-01

    Methods for detecting the presence of Coxiella burenetii in biological samples, as well as a method for differentiating strains of C. burnetii that are capable of causing acute disease from those strains capable of causing chronic disease are disclosed. The methods generally comprise treating cells contained within the biological sample to expose cellular DNA, and hybridizing the cellular DNA (specifically rickettsial DNA) with a C. burnetii-specific labeled DNA probe. Radioisotope and biotin labels are preferred, allowing detection through autoradiography and colorimetric assays, respectively.

  2. Detection and differentiation of coxiella burnetii in biological fluids

    DOE Patents [OSTI]

    Frazier, Marvin E.; Mallavia, Louis P.; Samuel, James E.; Baca, Oswald G.

    1993-01-01

    Methods for detecting the presence of Coxiella burnetii in biological samples, as well as a method for differentiating strains of C. burnetii that are capable of causing acute disease from those strains capable of causing chronic disease are disclosed. The methods generally comprise treating cells contained within the biological sample to expose cellular DNA, and hybridizing the cellular DNA with a DNA probe containing DNA sequences that specifically hybridize with C. burnetii DNA of strains associated with the capacity to cause acute or chronic disease.

  3. Detection and differentiation of coxiella burnetii in biological fluids

    DOE Patents [OSTI]

    Frazier, Marvin E.; Mallavia, Louis P.; Baca, Oswald G.; Samuel, James E.

    1989-01-01

    Methods for detecting the presence of Coxiella burnetii in biological samples, as well as a method for differentiating strains of C. burnetii that are capable of causing acute disease from those strains capable of causing chronic disease are disclosed. The methods generally comprise treating cells contained within the biological sample to expose cellular DNA, and hybridizing the cellular DNA (specifically rickettsial DNA) with a C. burnetii-specific labeled DNA probe. Radioisotope and biotin labels are preferred, allowing detection through autoradiography and colorimetric assays, respectively.

  4. Biological optimization systems for enhancing photosynthetic efficiency and methods of use

    DOE Patents [OSTI]

    Hunt, Ryan W; Chinnasamy, Senthil; Das, Keshav C; Rolim de Mattos, Erico

    2014-02-25

    The present disclosure relates to biological optimization systems for enhancing photosynthetic efficiency and methods of use.

  5. Behavior of REE in geological and biological systems

    SciTech Connect (OSTI)

    Laul, J.C.; Weimer, W.C.

    1981-05-01

    The REE abundances when normalized to primordial (chondritic) abundances behave as a smooth function of the REE ionic radii in both the geological and biological systems. The REE are hardly fractionated chemically through various stages of their transformation from soil-soil extract-plant-geological systems.

  6. Computational approaches to discovering semantics in molecular biology

    SciTech Connect (OSTI)

    Lipton, R.J. ); Marr, T.G. . Theoretical Div.); Welsh, J.D. . Dept. of Biology)

    1989-07-01

    One of the central questions of molecular biology is the discovery of the semantics of DNA. This discovery relies in a critical way on a variety of expensive computations. In order to solve these computations, both parallel computers and special-purpose hardware play a major role.

  7. PG&E Reconductoring Project Biological Assessment (Revised)

    Broader source: Energy.gov [DOE]

    This biological assessment (BA) is to provide the information necessary for formal consultation between the Department of Energy (DOE) and U.S. Fish and Wildlife Service (USFWS) regarding the effects of the Proposed Action as required pursuant to Section 7(a)(2) of the Endangered Species Act (ESA).

  8. Biological Conversion of Sugars to Hydrocarbons Technology Pathway

    Broader source: Energy.gov [DOE]

    This technology pathway case investigates the biological conversion of biomass-derived sugars to hydrocarbon biofuels, utilizing data from recent literature references and information consistent with recent pilot-scale demonstrations at NREL. Technical barriers and key research needs have been identified that should be pursued for the pathway to become competitive with petroleum-derived gasoline-, diesel-, and jet-range hydrocarbon blendstocks.

  9. Biologically Enhanced Carbon Sequestration: Research Needs and Opportunities

    SciTech Connect (OSTI)

    Oldenburg, Curtis; Oldenburg, Curtis M.; Torn, Margaret S.

    2008-03-21

    Fossil fuel combustion, deforestation, and biomass burning are the dominant contributors to increasing atmospheric carbon dioxide (CO{sub 2}) concentrations and global warming. Many approaches to mitigating CO{sub 2} emissions are being pursued, and among the most promising are terrestrial and geologic carbon sequestration. Recent advances in ecology and microbial biology offer promising new possibilities for enhancing terrestrial and geologic carbon sequestration. A workshop was held October 29, 2007, at Lawrence Berkeley National Laboratory (LBNL) on Biologically Enhanced Carbon Sequestration (BECS). The workshop participants (approximately 30 scientists from California, Illinois, Oregon, Montana, and New Mexico) developed a prioritized list of research needed to make progress in the development of biological enhancements to improve terrestrial and geologic carbon sequestration. The workshop participants also identified a number of areas of supporting science that are critical to making progress in the fundamental research areas. The purpose of this position paper is to summarize and elaborate upon the findings of the workshop. The paper considers terrestrial and geologic carbon sequestration separately. First, we present a summary in outline form of the research roadmaps for terrestrial and geologic BECS. This outline is elaborated upon in the narrative sections that follow. The narrative sections start with the focused research priorities in each area followed by critical supporting science for biological enhancements as prioritized during the workshop. Finally, Table 1 summarizes the potential significance or 'materiality' of advances in these areas for reducing net greenhouse gas emissions.

  10. Physics in medicine and biology encyclopedia 2-volume set

    SciTech Connect (OSTI)

    McAinsh, T.F.

    1986-01-01

    The Physics in Medicine and Biology Encyclopedia provides in two volumes a review of a wide range of topics of current interest in medical physics, bioengineering and biophysics. The Encyclopedia is extensively cross-referenced, indexed and illustrated, and all articles are complete with fully verified bibliographies. A glossary gives full details of specialized terms appearing in the Encyclopedia.

  11. Department of Defense Nuclear/Biological/Chemical (NBC) warfare defense. Annual report to Congress, June 1994. Final report, 1 October 1992-30 September 1993

    SciTech Connect (OSTI)

    Not Available

    1994-06-01

    The National Defense Authorization Act for Fiscal Year 1994, Public Law 103-160, Title XVII, Chemical and Biological Weapons Defense, section 1703, directed the Secretary of Defense to submit an assessment and a description of plans to improve readiness. The DoD objective is to enable our forces to survive, fight and win in NBC contaminated environments. Discussed are new management objectives impacted by declining resources and force structure versus an ever changing threat environment. Nuclear biological, Chemical, NBC, Defense, Logistics, Readiness, Training, Contamination avoidance, Protection, Decontamination.

  12. coherent-structures-html

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Role of Coherent Structures in Scour Process Around Bridge Piers and Abutments

  13. Biological Redox Cycling Of Iron In Nontronite And Its Potential Application In Nitrate Removal

    SciTech Connect (OSTI)

    Zhao, Linduo; Dong, Hailiang; Kukkadapu, Ravi K.; Zeng, Qiang; Edelmann, Richard E.; Pentrak, Martin; Agrawal, Abinash

    2015-05-05

    Redox cycling of structural Fe in phyllosilicates provides a potential method to remediate nitrate contamination in natural environment. Past research has only studied chemical redox cycles or a single biologically mediated redox cycle of Fe in phyllosilicates. The objective of this research was to study three microbially driven redox cycles of Fe in one phyllosilicate, nontronite (NAu-2). During the reduction phase structural Fe(III) in NAu-2 served as electron acceptor, lactate as electron donor, AQDS as electron shuttle, and dissimilatory Fe(III)-reducing bacteria Shewanella putrefaciens CN32 as mediator in bicarbonate-buffered and PIPES-buffered media. During the oxidation phase, biogenic Fe(II) served an electron donor, nitrate as electron acceptor, and nitrate-dependent Fe(II)-oxidizing bacteria Pseudogulbenkiania sp. strain 2002 as mediator in the same media. For all three cycles, structural Fe in NAu-2 was able to reversibly undergo 3 redox cycles without significant reductive or oxidative dissolution. X-ray diffraction and scanning and transmission electron microscopy revealed that NAu-2 was the dominant residual mineral throughout the 3 redox cycles with some dissolution textures but no significant secondary mineralization. Mssbauer spectroscopy revealed that Fe(II) in bio-reduced samples likely occurred in two distinct environments, at edges and the interior of the NAu-2 structure. Nitrate was completely reduced to nitrogen gas under both buffer conditions and this extent and rate did not change with Fe redox cycles. Mssbauer spectroscopy further revealed that nitrate reduction was coupled to predominant/preferred oxidation of edge Fe(II). These results suggest that structural Fe in phyllosilicates may represent a renewable source to continuously remove nitrate in natural environments.

  14. Development and Applications Of Photosensitive Device Systems To Studies Of Biological And Organic Materials

    SciTech Connect (OSTI)

    Gruner, Sol

    2012-01-20

    The primary focus of the grant is the development of new x-ray detectors for biological and materials work at synchrotron sources, especially Pixel Array Detectors (PADs), and the training of students via research applications to problems in biophysics and materials science using novel x-ray methods. This Final Progress Report provides a high-level overview of the most important accomplishments. These major areas of accomplishment include: (1) Development and application of x-ray Pixel Array Detectors; (2) Development and application of methods of high pressure x-ray crystallography as applied to proteins; (3) Studies on the synthesis and structure of novel mesophase materials derived from block co-polymers.

  15. Final Report - Phylogenomic tools and web resources for the Systems Biology Knowledgebase

    SciTech Connect (OSTI)

    Sjolander, Kimmen

    2014-11-07

    The major advance during this last reporting period (8/15/12 to present) is our release of data on the PhyloFacts website: phylogenetic trees, multiple sequence alignments and other data for protein families are now available for download from http://phylogenomics.berkeley.edu/data/. This project as a whole aimed to develop high-throughput functional annotation systems that exploit information from protein 3D structure and evolution to provide highly precise inferences of various aspects of gene function, including molecular function, biological process, pathway association, Pfam domains, cellular localization and so on. We accomplished these aims by developing and testing different systems on a database of protein family trees: the PhyloFacts Phylogenomic Encyclopedia (at http://phylogenomics.berkeley.edu/phylofacts/ ).

  16. Mueller-matrix mapping of optically anisotropic fluorophores of biological tissues in the diagnosis of cancer

    SciTech Connect (OSTI)

    Ushenko, Yu A; Sidor, M I; Bodnar, G B; Koval', G D

    2014-08-31

    We report the results of studying the polarisation manifestations of laser autofluorescence of optically anisotropic structures in biological tissues. A Mueller-matrix model is proposed to describe their complex anisotropy (linear and circular birefringence, linear and circular dichroism). The relationship is established between the mechanisms of optical anisotropy and polarisation manifestations of laser autofluorescence of histological sections of rectal tissue biopsy in different spectral regions. The ranges of changes in the statistical moments of the 1st-to-4th orders, which describe the distribution of the azimuth-invariant elements of Mueller matrices of rectal tissue autofluorescence, are found. Effectiveness of laser autofluorescence polarimetry is determined and the histological sections of biopsy of benign (polyp) and malignant (adenocarcinoma) tumours of the rectal wall are differentiated for the first time. (laser biophotonics)

  17. Chemical and biological warfare: Biology, chemistry, and toxicology. (Latest citations from the NTIS bibliographic database). Published Search

    SciTech Connect (OSTI)

    1996-10-01

    The bibliography contains citations concerning the physiological effects, physicochemical effects, and toxicology of chemical and biological warfare agents. Citations discuss toxic chemicals, chemical agent simulants, detoxification and decontamination, environmental toxicity, and land pollution. Detection techniques and warning systems are examined in a separate bibliography. (Contains 50-250 citations and includes a subject term index and title list.) (Copyright NERAC, Inc. 1995)

  18. Chemical and biological warfare: Biology, chemistry, and toxicology. (Latest citations from the NTIS bibliographic database). Published Search

    SciTech Connect (OSTI)

    1995-10-01

    The bibliography contains citations concerning the physiological effects, physicochemical effects, and toxicology of chemical and biological warfare agents. Citations discuss toxic chemicals, chemical agent simulants, detoxification and decontamination, environmental toxicity, and land pollution. Detection techniques and warning systems are examined in a separate bibliography. (Contains 50-250 citations and includes a subject term index and title list.) (Copyright NERAC, Inc. 1995)

  19. Chemical and biological warfare: Biology, chemistry, and toxicology. (Latest citations from the NTIS bibliographic database). Published Search

    SciTech Connect (OSTI)

    1995-03-01

    The bibliography contains citations concerning the physiological effects, physicochemical effects, and toxicology of chemical and biological warfare agents. Citations discuss toxic chemicals, chemical agent simulants, detoxification and decontamination, environmental toxicity, and land pollution. Detection techniques and warning systems are examined in a separate bibliography. (Contains a minimum of 229 citations and includes a subject term index and title list.)

  20. Chemical and biological warfare: Biology, chemistry, and toxicology. (Latest citations from the NTIS bibliographic database). Published Search

    SciTech Connect (OSTI)

    1997-11-01

    The bibliography contains citations concerning the physiological effects, physicochemical effects, and toxicology of chemical and biological warfare agents. Citations discuss toxic chemicals, chemical agent simulants, detoxification and decontamination, environmental toxicity, and land pollution. Detection techniques and warning systems are examined in a separate bibliography. (Contains 50-250 citations and includes a subject term index and title list.) (Copyright NERAC, Inc. 1995)

  1. Biological and Biomimetic Low-Temperature Routes to Materials...

    Broader source: Energy.gov (indexed) [DOE]

    Nano-scale Composite Hetero-structures: Novel High Capacity Reversible Anodes for Lithium-ion Batteries Novel Lithium Ion Anode Structures: Overview of New DOE BATT Anode Projects ...

  2. Quality engineering as a discipline of study.

    SciTech Connect (OSTI)

    Kolb, Rachel R.; Hoover, Marcey L.

    2012-12-01

    The current framework for quality scholarship in the United States ranges from the training and education of future quality engineers, managers, and professionals to focused and sustained research initiatives that, through academic institutions and other organizations, aim to improve the knowledge and application of quality across a variety of sectors. Numerous quality journals also provide a forum for professional dissemination of information.

  3. Administering Workforce Discipline (Informational Purposes Only)

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2015-01-26

    This draft has been scheduled for final review before the Directives Review Board on 02/5/2015. All major comments and concerns should be provided to your DRB representative by 02/3/2015, following your organization process. If you do not know who your representative is, please see the list of DRB members. If your office is represented by Ingrid Kolb, Director, Office of Management, please submit your major concerns and comments to the DRB Liaison, Camille Beben (Camille.Beben@hq.doe.gov; 202-586-1014).

  4. DOE Chair Excellence Professorship Environmental Disciplines

    SciTech Connect (OSTI)

    Riley, Reginald

    2014-10-08

    The DECM Team worked closely with other academic institutions, industrial companies and government laboratories to do research and educate engineers in “cutting edge” environmentally conscious manufacturing practices and instrumentation. The participating universities also worked individually with local companies on research projects in their specialty areas. Together, they were charged with research application, integration and education in environmentally conscious manufacturing.

  5. Differential Function of Lip Residues in the Mechanism and Biology of an Anthrax Hemophore

    SciTech Connect (OSTI)

    Ekworomadu, MarCia T.; Poor, Catherine B.; Owens, Cedric P.; Balderas, Miriam A.; Fabian, Marian; Olson, John S.; Murphy, Frank; Balkabasi, Erol; Honsa, Erin S.; He, Chuan; Goulding, Celia W.; Maresso, Anthony W.

    2014-10-02

    To replicate in mammalian hosts, bacterial pathogens must acquire iron. The majority of iron is coordinated to the protoporphyrin ring of heme, which is further bound to hemoglobin. Pathogenic bacteria utilize secreted hemophores to acquire heme from heme sources such as hemoglobin. Bacillus anthracis, the causative agent of anthrax disease, secretes two hemophores, IsdX1 and IsdX2, to acquire heme from host hemoglobin and enhance bacterial replication in iron-starved environments. Both proteins contain NEAr-iron Transporter (NEAT) domains, a conserved protein module that functions in heme acquisition in Gram-positive pathogens. Here, we report the structure of IsdX1, the first of a Gram-positive hemophore, with and without bound heme. Overall, IsdX1 forms an immunoglobin-like fold that contains, similar to other NEAT proteins, a 3{sub 10}-helix near the heme-binding site. Because the mechanistic function of this helix in NEAT proteins is not yet defined, we focused on the contribution of this region to hemophore and NEAT protein activity, both biochemically and biologically in cultured cells. Site-directed mutagenesis of amino acids in and adjacent to the helix identified residues important for heme and hemoglobin association, with some mutations affecting both properties and other mutations affecting only heme stabilization. IsdX1 with mutations that reduced the ability to associate with hemoglobin and bind heme failed to restore the growth of a hemophore-deficient strain of B. anthracis on hemoglobin as the sole iron source. These data indicate that not only is the 3{sub 10}-helix important for NEAT protein biology, but also that the processes of hemoglobin and heme binding can be both separate as well as coupled, the latter function being necessary for maximal heme-scavenging activity. These studies enhance our understanding of NEAT domain and hemophore function and set the stage for structure-based inhibitor design to block NEAT domain interaction with upstream ligands.

  6. Update on the Pfam5000 Strategy for Selection of StructuralGenomics Targets

    Office of Scientific and Technical Information (OSTI)

    (Conference) | SciTech Connect Conference: Update on the Pfam5000 Strategy for Selection of StructuralGenomics Targets Citation Details In-Document Search Title: Update on the Pfam5000 Strategy for Selection of StructuralGenomics Targets Structural Genomics is an international effort to determine the three-dimensional shapes of all important biological macromolecules, with a primary focus on proteins. Target proteins should be selected according to a strategy that is medically and

  7. Automating the determination of 3D protein structure

    SciTech Connect (OSTI)

    Rayl, K.D.

    1993-12-31

    The creation of an automated method for determining 3D protein structure would be invaluable to the field of biology and presents an interesting challenge to computer science. Unfortunately, given the current level of protein knowledge, a completely automated solution method is not yet feasible, therefore, our group has decided to integrate existing databases and theories to create a software system that assists X-ray crystallographers in specifying a particular protein structure. By breaking the problem of determining overall protein structure into small subproblems, we hope to come closer to solving a novel structure by solving each component. By generating necessary information for structure determination, this method provides the first step toward designing a program to determine protein conformation automatically.

  8. Biological responses to engineered nanomaterials: Needs for the next decade

    SciTech Connect (OSTI)

    Murphy, Catherine J.; Vartanian, Ariane M.; Geiger, Franz M.; Hamers, Robert J.; Pedersen, Joel A.; Cui, Qiang; Haynes, Christy L.; Carlson, Erin E.; Hernandez, Rigoberto; Klaper, Rebecca D.; Orr, Galya; Rosenzweig, Ze'ev

    2015-06-09

    In this study, the interaction of nanomaterials with biomolecules, cells, and organisms is an enormously vital area of current research, with applications in nanoenabled diagnostics, imaging agents, therapeutics, and contaminant removal technologies. Yet the potential for adverse biological and environmental impacts of nanomaterial exposure is considerable and needs to be addressed to ensure sustainable development of nanomaterials. In this Outlook four research needs for the next decade are outlined: (i) measurement of the chemical nature of nanomaterials in dynamic, complex aqueous environments; (ii) real-time measurements of nanomaterial-biological interactions with chemical specificity; (iii) delineation of molecular modes of action for nanomaterial effects on living systems as functions of nanomaterial properties; and (iv) an integrated systems approach that includes computation and simulation across orders of magnitude in time and space.

  9. Biological responses to engineered nanomaterials: Needs for the next decade

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Murphy, Catherine J.; Vartanian, Ariane M.; Geiger, Franz M.; Hamers, Robert J.; Pedersen, Joel A.; Cui, Qiang; Haynes, Christy L.; Carlson, Erin E.; Hernandez, Rigoberto; Klaper, Rebecca D.; et al

    2015-06-09

    In this study, the interaction of nanomaterials with biomolecules, cells, and organisms is an enormously vital area of current research, with applications in nanoenabled diagnostics, imaging agents, therapeutics, and contaminant removal technologies. Yet the potential for adverse biological and environmental impacts of nanomaterial exposure is considerable and needs to be addressed to ensure sustainable development of nanomaterials. In this Outlook four research needs for the next decade are outlined: (i) measurement of the chemical nature of nanomaterials in dynamic, complex aqueous environments; (ii) real-time measurements of nanomaterial-biological interactions with chemical specificity; (iii) delineation of molecular modes of action for nanomaterialmore » effects on living systems as functions of nanomaterial properties; and (iv) an integrated systems approach that includes computation and simulation across orders of magnitude in time and space.« less

  10. Flow-through biological conversion of lignocellulosic biomass

    DOE Patents [OSTI]

    Herring, Christopher D.; Liu, Chaogang; Bardsley, John

    2014-07-01

    The present invention is directed to a process for biologically converting carbohydrates from lignocellulosic biomass comprising the steps of: suspending lignocellulosic biomass in a flow-through reactor, passing a reaction solution into the reactor, wherein the solution is absorbed into the biomass substrate and at least a portion of the solution migrates through said biomass substrate to a liquid reservoir, recirculating the reaction solution in the liquid reservoir at least once to be absorbed into and migrate through the biomass substrate again. The biological converting of the may involve hydrolyzing cellulose, hemicellulose, or a combination thereof to form oligosaccharides, monomelic sugars, or a combination thereof; fermenting oligosaccharides, monomelic sugars, or a combination thereof to produce ethanol, or a combination thereof. The process can further comprise removing the reaction solution and processing the solution to separate the ethanol produced from non-fermented solids.

  11. Using the Gene Ontology to Enrich Biological Pathways

    SciTech Connect (OSTI)

    Sanfilippo, Antonio P.; Baddeley, Robert L.; Beagley, Nathaniel; McDermott, Jason E.; Riensche, Roderick M.; Taylor, Ronald C.; Gopalan, Banu

    2009-12-10

    Most current approaches to automatic pathway generation are based on a reverse engineering approach in which pathway plausibility is solely derived from microarray gene expression data. These approaches tend to lack in generality and offer no independent validation as they are too reliant on the pathway observables that guide pathway generation. By contrast, alternative approaches that use prior biological knowledge to validate pathways inferred from gene expression data may err in the opposite direction as the prior knowledge is usually not sufficiently tuned to the pathology of focus. In this paper, we present a novel pathway generation approach that combines insights from the reverse engineering and knowledge-based approaches to increase the biological plausibility of automatically generated regulatory networks and describe an application of this approach to transcriptional data from a mouse model of neuroprotection during stroke.

  12. Towards a Semantic Lexicon for Biological Language Processing

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Verspoor, Karin

    2005-01-01

    This paper explores the use of the resources in the National Library of Medicine's Unified Medical Language System (UMLS) for the construction of a lexicon useful for processing texts in the field of molecular biology. A lexicon is constructed from overlapping terms in the UMLS SPECIALIST lexicon and the UMLS Metathesaurus to obtain both morphosyntactic and semantic information for terms, and the coverage of a domain corpus is assessed. Over 77% of tokens in the domain corpus are found in the constructed lexicon, validating the lexicon's coverage of the most frequent terms in the domain and indicating that the constructedmore » lexicon is potentially an important resource for biological text processing.« less

  13. Synthetic Biology: Engineering, Evolution and Design (SEED) Conference 2014

    SciTech Connect (OSTI)

    Voigt, Christopher

    2014-07-01

    SEED2014 focused on advances in the science and technology emerging from the field of synthetic biology. We broadly define this as technologies that accelerate the process of genetic engineering. It highlighted new tool development, as well as the application of these tools to diverse problems in biotechnology, including therapeutics, industrial chemicals and fuels, natural products, and agriculture. Systems spanned from in vitro experiments and viruses, through diverse bacteria, to eukaryotes (yeast, mammalian cells, plants).

  14. In Situ Biological Uranium Remediation within a Highly Contaminated Aquifer

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In Situ Biological Uranium Remediation within a Highly Contaminated Aquifer Matthew Ginder-Vogel1, Wei-Min Wu1, Jack Carley2, Phillip Jardine2, Scott Fendorf1 and Craig Criddle1 1Stanford University, Stanford, CA 2Oak Ridge National Laboratory, Oak Ridge, TN Microbial Respiration Figure 1. Uranium(VI) reduction is driven by microbial respiration resulting in the precipitation of uraninite. Uranium contamination of ground and surface waters has been detected at numerous sites throughout the

  15. American Society of Agricultural and Biological Engineers Annual International Meeting

    Broader source: Energy.gov [DOE]

    The 2015 American Society of Agricultural and Biological Engineers Annual International Meeting will be held in New Orleans, Louisiana on July 26–29, 2015, and will examine industry trends and innovations, with a focus on the focus on the economic, political and social factors influencing the industry. Bioenergy Technologies Office Director Jonathan Male, Program Manager Alison Goss Eng, and Technology Managers Sam Tagore, Mark Elless, and Steve Thomas will be in attendance.

  16. Biological Imaging by Soft X-Ray Diffraction Microscopy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Biological Imaging by Soft X-Ray Diffraction Microscopy Print Electron and x-ray microscopes are a valuable tool for both the life and materials sciences, but they are limited in their ability to image with nanometer-scale resolution in three dimensions nonperiodic objects that are several microns in size. To fill this gap, the technique of coherent x-ray diffraction imaging now under development takes advantage of the penetrating power of x rays while simultaneously removing the limitations

  17. Biological Imaging by Soft X-Ray Diffraction Microscopy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Biological Imaging by Soft X-Ray Diffraction Microscopy Print Electron and x-ray microscopes are a valuable tool for both the life and materials sciences, but they are limited in their ability to image with nanometer-scale resolution in three dimensions nonperiodic objects that are several microns in size. To fill this gap, the technique of coherent x-ray diffraction imaging now under development takes advantage of the penetrating power of x rays while simultaneously removing the limitations

  18. Biological Imaging by Soft X-Ray Diffraction Microscopy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Biological Imaging by Soft X-Ray Diffraction Microscopy Print Electron and x-ray microscopes are a valuable tool for both the life and materials sciences, but they are limited in their ability to image with nanometer-scale resolution in three dimensions nonperiodic objects that are several microns in size. To fill this gap, the technique of coherent x-ray diffraction imaging now under development takes advantage of the penetrating power of x rays while simultaneously removing the limitations

  19. Biological Imaging by Soft X-Ray Diffraction Microscopy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Biological Imaging by Soft X-Ray Diffraction Microscopy Print Electron and x-ray microscopes are a valuable tool for both the life and materials sciences, but they are limited in their ability to image with nanometer-scale resolution in three dimensions nonperiodic objects that are several microns in size. To fill this gap, the technique of coherent x-ray diffraction imaging now under development takes advantage of the penetrating power of x rays while simultaneously removing the limitations

  20. Biological Imaging by Soft X-Ray Diffraction Microscopy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Biological Imaging by Soft X-Ray Diffraction Microscopy Print Electron and x-ray microscopes are a valuable tool for both the life and materials sciences, but they are limited in their ability to image with nanometer-scale resolution in three dimensions nonperiodic objects that are several microns in size. To fill this gap, the technique of coherent x-ray diffraction imaging now under development takes advantage of the penetrating power of x rays while simultaneously removing the limitations