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  1. Welcome Rachel Mack!

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    Rachel Mack, the newest member of the directives team came on board May 4, 2015. Rachel, a United States Army veteran, recently graduated from Georgetown University with a Masters Degree in Public Policy. She will be assisting Camille Beben and Christopher Tirado in administering the directives program.

  2. Mack LNG vehicle development

    SciTech Connect (OSTI)

    Southwest Research Institute

    2000-01-05

    The goal of this project was to install a production-ready, state-of-the-art engine control system on the Mack E7G natural gas engine to improve efficiency and lower exhaust emissions. In addition, the power rating was increased from 300 brake horsepower (bhp) to 325 bhp. The emissions targets were oxides of nitrogen plus nonmethane hydrocarbons of less than 2.5 g/bhp-hr and particulate matter of less than 0.05 g/bhp-hr on 99% methane. Vehicle durability and field testing were also conducted. Further development of this engine should include efficiency improvements and oxides of nitrogen reductions.

  3. ORISE: Graduate Student Research Experiences - Yashema Mack

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Yashema Mack analyzed data from the world' fastest supercomputer, putting her computer skills to use for nuclear technology advancement as part of an Oak Ridge National Laboratory...

  4. Solar Engineering Decker Mack GmbH | Open Energy Information

    Open Energy Info (EERE)

    Engineering Decker Mack GmbH Jump to: navigation, search Name: Solar Engineering Decker & Mack GmbH Place: Hannover, Germany Zip: 30165 Sector: Services, Solar Product: Firm...

  5. U.S. Energy Information Administration | Annual Energy Outlook...

    Annual Energy Outlook [U.S. Energy Information Administration (EIA)]

    Centr al (4) W. South Centr al (7) E. South Centr al (6) E. North Centr al (3) S. Atlan tic (5) FL (10) Mid. Atlan tic (2) New Engl. (1) W. Canad a E. Canad a MacK enzie Alask a...

  6. Annual Energy Outlook 2015 - Appendix F

    Gasoline and Diesel Fuel Update (EIA)

    6 Regional maps Figure F5. Natural gas transmission and distribution model regions 218 U.S. Energy Information Administration / Annual Energy Outlook 2010 Figure F5. Natural Gas Transmission and Distribution Model Regions Pacifi c (9) Moun tain (8) CA (12) AZ/N M (11) W. North Centr al (4) W. South Centr al (7) E. South Centr al (6) E. North Centr al (3) S. Atlan tic (5) FL (10) Mid. Atlan tic (2) New Engl. (1) W. Canad a E. Canad a MacK enzie Alask a Canad a Offsh ore and LNG Mexic o Baham as

  7. Energetica Serra da Prata | Open Energy Information

    Open Energy Info (EERE)

    da Prata Jump to: navigation, search Name: Energetica Serra da Prata Place: Bahia, Brazil Product: SHP developer based in the state of Bahia, Brazil. References: Energetica Serra...

  8. Biopalma da Amaz nia | Open Energy Information

    Open Energy Info (EERE)

    da Amaznia Place: Belem, Para, Brazil Product: Brazilian palm oil plantation for food industry developer company. Coordinates: -1.454426, -48.502537 Show Map Loading...

  9. Eolica Cajueiro da Praia Ltda | Open Energy Information

    Open Energy Info (EERE)

    Eolica Cajueiro da Praia Ltda Jump to: navigation, search Name: Eolica Cajueiro da Praia Ltda Place: Fortaleza, Ceara, Brazil Zip: 60170-251 Sector: Renewable Energy, Wind energy...

  10. Mercado Abastecedor da Regiao de Lisboa MARL | Open Energy Information

    Open Energy Info (EERE)

    Mercado Abastecedor da Regiao de Lisboa MARL Jump to: navigation, search Name: Mercado Abastecedor da Regiao de Lisboa (MARL) Place: Lisbon, Portugal Zip: 2660-421 Product: Mercado...

  11. Incubadora de Empresas da Universidade de Aveiro IEUA | Open...

    Open Energy Info (EERE)

    Incubadora de Empresas da Universidade de Aveiro IEUA Jump to: navigation, search Name: Incubadora de Empresas da Universidade de Aveiro (IEUA) Place: Portugal Sector: Services...

  12. DA (Distribution Automation) (Smart Grid Project) | Open Energy...

    Open Energy Info (EERE)

    DA (Distribution Automation) (Smart Grid Project) Jump to: navigation, search Project Name DA (Distribution Automation) Country Netherlands Coordinates 52.132633, 5.291266...

  13. Data Assimilation (UQ/DA) Study

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Uncertainty Quantification and Data Assimilation (UQ/DA) Study on a VERA Core Simulator Component for CRUD Analysis Hany S. Abdel-Khalik North Carolina State University Reviewed by J. Michael Doster (NCSU) Rod Schmidt (SNL) August 30, 2013 CASL-U-2013-0184-000 Milestone Report for L2:VUQ:P7.02 Uncertainty Quantification and Data Assimilation (UQ/DA) Study on a VERA Core Simulator Component for CRUD Analysis CASL-I-2013-0184-000 Jason M. Hite 1 , Hany S. Abdel-Khalik 1 , Ralph C. Smith 2 , Mami

  14. STEAB Teleconference Minutes November 2007

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Vincent, Avista Utilities, Oregon - Alexander Mack, Manager, State Energy Program, ... Agents in the Dissemination of Energy- Education Materials" Alexander Mack introduced Mr. ...

  15. STEAB Teleconference Minutes June 2007

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Vincent, Avista Utilities, Oregon - Alexander Mack, Manager, State Energy Program, ... Development "white paper" based on these changes and Alexander Mack seconded the motion. ...

  16. 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Characterization of Mixed-Phase Clouds During Mixed-Phase Arctic Cloud Experiment from Satellite, Ground-Based, and In-Situ Data D.A. Spangenberg Analytical Services & Materials, Inc. Hampton, Virginia P. Minnis National Aeronautics and Space Agency - Langley Research Center Hampton, Virginia S. Sun-Mack Science Applications International Corporation Hampton, Virginia M.D. Shupe Cooperative Institute for Research in Environmental Science, National Oceanic and Atmospheric Administration -

  17. PLEAEERUSH ANALYTICAL DA-~-A SHEET

    Office of Legacy Management (LM)

    ' PLEAEERUSH ANALYTICAL DA-~-A SHEET ' ANALYTICAL DEPT. - HEALTH AhD SAFETY DlVlSlON 1956 Industrial Hygiene or Medical Dept. 1. H.#~~Sample Nos. 3 --Date Collected~~by-CESS-.Route to CBS LocationTITANIUM Type of Sample airnalyzed for F Alpham Remarks NIAGARA pALI+S* N.Y. U Beta Bldg. 103 - furnace room - -NO, Ra Oil PH Be Th Sample No. Hour Sample Description I I I--- R ) T 1 Q I I I 7392 1100 GA Induction furnace area duri-nn ----l----- mDeriod;.02; 151 .3 while furnace was charged with UOT_-

  18. Eletricidade da Amaz nia S A ELETRAM | Open Energy Information

    Open Energy Info (EERE)

    Amaz nia S A ELETRAM Jump to: navigation, search Name: Eletricidade da Amaznia SA (ELETRAM) Place: Cuiaba, Mato Grosso, Brazil Zip: 78040-570 Sector: Hydro Product: Small...

  19. CoDA 2016, the Conference on Data Analysis

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    March » CoDA 2016, the Conference on Data Analysis CoDA 2016, the Conference on Data Analysis WHEN: Mar 02, 2016 8:00 AM - Mar 04, 2016 5:00 PM WHERE: Eldorado Hotel 309 W. San Francisco St., Santa Fe, NM CONTACT: Kary Myers (505) 606-1455 CATEGORY: Science TYPE: Conference INTERNAL: Calendar Login Event Description Join us for the Conference on Data Analysis, highlighting data-driven problems of interest to the Department of Energy. We invite you to present your data-focused work at the poster

  20. Tennessee: Da Vinci Fuel-in-Oil Reduces Emissions, Wins R&D 100...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Tennessee: Da Vinci Fuel-in-Oil Reduces Emissions, Wins R&D 100 Award Tennessee: Da Vinci Fuel-in-Oil Reduces Emissions, Wins R&D 100 Award August 19, 2013 - 5:07pm Addthis ...

  1. Funda o Parque Tecnol gico da Paraiba PaqTc Incubator | Open...

    Open Energy Info (EERE)

    Funda o Parque Tecnol gico da Paraiba PaqTc Incubator Jump to: navigation, search Name: Fundao Parque Tecnolgico da Paraiba (PaqTc Incubator) Place: Brazil Sector: Services...

  2. Tennessee: Da Vinci Fuel-in-Oil Reduces Emissions, Wins R&D 100...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Tennessee: Da Vinci Fuel-in-Oil Reduces Emissions, Wins R&D 100 Award Tennessee: Da Vinci Fuel-in-Oil Reduces Emissions, Wins R&D 100 Award August 19, 2013 - 5:07pm Addthis...

  3. EERE Success Story-Tennessee: Da Vinci Fuel-in-Oil Reduces Emissions...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Tennessee: Da Vinci Fuel-in-Oil Reduces Emissions, Wins R&D 100 Award EERE Success Story-Tennessee: Da Vinci Fuel-in-Oil Reduces Emissions, Wins R&D 100 Award August 19, 2013 - ...

  4. Roles and Delegation of Authority (R/DA) System

    SciTech Connect (OSTI)

    ABBOTT,JOHN P.; HUTCHINS,JAMES C.; SCHOCH,DAVID G.

    1999-11-01

    The processes of defining managerial roles and providing for delegation of authority are essential to any enterprise. At most large organizations, these processes are defined in policy manuals and through sets of standard operating procedures for many, if not all, business and administrative functions. Many of these staff-initiated, administrative functions require the routing of documents for approval to one or more levels of management. These employee-oriented, back office types of workflows tend to require more flexibility in determining to whom these documents should go to, while, at the same time, providing the responsible parties with the flexibility to delegate their approval authority or allow others to review their work. Although this practice is commonplace in manual, paper-based processes that exist in many organizations, it is difficult to provide the same flexibility in the more structured, electronic-based, workflow systems. The purpose of this report is to present a framework or architecture for creating a R/DA system and provide some insights associated with its design and utilization. To improve understanding and clarify subsequent discussion, the goals and requirements for the major R/DA system components, namely, the database and interface modules, are initially presented along with the identification of important concepts and the definition of critical terms. Next high-level functions relating the types of inputs to the outputs of the R/DA interface module are introduced and discussed. Then the relationships between the major R/DA modules and the primary components associated with its creation and maintenance are presented and analyzed. Finally, some conclusions are drawn relative to the advantages associated with developing a R/DA system for use in implementing an enterprise-wide, work-facilitating information system.

  5. dI UNIVERSITY OF NEV\DA SYSTEM

    Office of Legacy Management (LM)

    g3t4 6 dI UNIVERSITY OF NEV\DA SYSTEM tw ?r@ D O E / D P / O 1 2 6 3 - 2 0 L , n z l t P ' " WATER RESOURCES CENTER itf.l This report was prepared as an aecount of work sponsore$ by the United States Government. Neither the United States nor the United States Department of Energy, nor any of their employees, mal assumes any legal liability or responsib usefulness of any informationr apparatus' I that its use would not infringe privately speeifie eommereial produetr proeesst ufacturen, or

  6. STEAB Teleconference Minutes May 2007

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Institutions (ASERTTI), Virginia - Steve Vincent, Avista Utilities, Oregon - Alexander Mack, Manager, State Energy Program, Florida Energy Office - Peter Johnston, Manager, ...

  7. Tennessee: Da Vinci Fuel-in-Oil Reduces Emissions, Wins R&D 100 Award

    Broader source: Energy.gov [DOE]

    Developed jointly by Da Vinci Emissions Services Ltd., Cummins Inc., and Oak Ridge National Laboratory (ORNL), the Da Vinci Fuel-in-Oil (DAFIO) technology uses a fiber optic probe to obtain real-time measurements of oil in an operating engine to quantify the fuel dissolved in the lubricant oil.

  8. 70 DA WHITE DWARFS IDENTIFIED IN LAMOST PILOT SURVEY

    SciTech Connect (OSTI)

    Zhao, J. K.; Luo, A. L.; Zhao, G. [Key Laboratory of Optical Astronomy, National Astronomical Observatories, Chinese Academy of Sciences, Beijing 100012 (China); Oswalt, T. D., E-mail: zjk@bao.ac.cn, E-mail: gzhao@bao.ac.cn, E-mail: lal@bao.ac.cn, E-mail: toswalt@fit.edu [Physics and Space Science Department, Florida Institute of Technology, Melbourne, FL 32901 (United States)

    2013-06-01

    We present a spectroscopically identified catalog of 70 DA white dwarfs (WDs) from the LAMOST pilot survey. Thirty-five are found to be new identifications after cross-correlation with the Eisenstein et al. and Villanova catalogs. The effective temperature and gravity of these WDs are estimated by Balmer lines fitting. Most of them are hot WDs. The cooling times and masses of these WDs are estimated by interpolation in theoretical evolution tracks. The peak of the mass distribution is found to be {approx}0.6 M {sub Sun }, which is consistent with prior work in the literature. The distances of these WDs are estimated using the method of synthetic spectral distances. All of these WDs are found to be in the Galactic disk from our analysis of space motions. Our sample supports the expectation that WDs with high mass are concentrated near the plane of the Galactic disk.

  9. PanDaTox: a tool for accelerated metabolic engineering (Journal Article) |

    Office of Scientific and Technical Information (OSTI)

    SciTech Connect PanDaTox: a tool for accelerated metabolic engineering Citation Details In-Document Search Title: PanDaTox: a tool for accelerated metabolic engineering Metabolic engineering is often facilitated by cloning of genes encoding enzymes from various heterologous organisms into E. coli. Such engineering efforts are frequently hampered by foreign genes that are toxic to the E. coli host. We have developed PanDaTox (www.weizmann.ac.il/pandatox), a web-based resource that provides

  10. Near-UV absorption in very cool DA white dwarfs

    SciTech Connect (OSTI)

    Saumon, D.; Holberg, J. B.; Kowalski, P. M. E-mail: holberg@argus.lpl.arizona.edu

    2014-07-20

    The atmospheres of very cool, hydrogen-rich white dwarfs (WDs) (T{sub eff} < 6000 K) are challenging to model because of the increased complexity of the equation of state, chemical equilibrium, and opacity sources in a low-temperature, weakly ionized dense gas. In particular, many models that assume relatively simple models for the broadening of atomic levels and mostly ideal gas physics overestimate the flux in the blue part of their spectra. A solution to this problem that has met with some success is that additional opacity at short wavelengths comes for the extreme broadening of the Lyman ? line of atomic H by collisions primarily with H{sub 2}. For the purpose of validating this model more rigorously, we acquired Hubble Space Telescope STIS spectra of eight very cool WDs (five DA and three DC stars). Combined with their known parallaxes, BVRIJHK, and Spitzer IRAC photometry, we analyze their entire spectral energy distribution (from 0.24 to 9.3 ?m) with a large grid of model atmospheres and synthetic spectra. We find that the red wing of the Lyman ? line reproduces the rapidly decreasing near-UV flux of these very cool stars very well. We determine better constrained values of T{sub eff} and gravity as well as upper limits to the helium abundance in their atmospheres.

  11. EERE Success Story—Tennessee: Da Vinci Fuel-in-Oil Reduces Emissions, Wins R&D 100 Award

    Broader source: Energy.gov [DOE]

    Developed jointly by Da Vinci Emissions Services Ltd., Cummins Inc., and Oak Ridge National Laboratory (ORNL), the Da Vinci Fuel-in-Oil (DAFIO™) technology uses a fiber optic probe to obtain real-time measurements of oil in an operating engine to quantify the fuel dissolved in the lubricant oil.

  12. STRESS AND FAILURE ANALYSIS OF RAPIDLY ROTATING ASTEROID (29075) 1950DA

    SciTech Connect (OSTI)

    Hirabayashi, Masatoshi; Scheeres, Daniel J.

    2015-01-01

    Rozitis et al. recently reported that near-Earth asteroid (29075) 1950DA, whose bulk density ranges from 1.0 g cm{sup 3} to 2.4g cm{sup 3}, is a rubble pile and requires a cohesive strength of at least 44-76 Pa to keep from failing due to its fast spin period. Since their technique for giving failure conditions required the averaged stress over the whole volume, it discarded information about the asteroid's failure mode and internal stress condition. This paper develops a finite element model and revisits the stress and failure analysis of 1950DA. For the modeling, we do not consider material hardening and softening. Under the assumption of an associated flow rule and uniform material distribution, we identify the deformation process of 1950DA when its constant cohesion reaches the lowest value that keeps its current shape. The results show that to avoid structural failure the internal core requires a cohesive strength of at least 75-85 Pa. It suggests that for the failure mode of this body, the internal core first fails structurally, followed by the surface region. This implies that if cohesion is constant over the whole volume, the equatorial ridge of 1950DA results from a material flow going outward along the equatorial plane in the internal core, but not from a landslide as has been hypothesized. This has additional implications for the likely density of the interior of the body.

  13. STATES GOVERI TO :H. J. He&man, Chief, Tonaw&da Sub-Of&e DATE...

    Office of Legacy Management (LM)

    STATES GOVERI TO :H. J. He&man, Chief, Tonaw&da Sub-Of&e DATE: ,i; .; .c. sgmbo1: PPS:W:mjf .. ,i. -'. i:.. :: (PPS447-53) I ..-:;..c. ' ..I-,-.. . i .,,. " :, ,, .T. ....

  14. Channeling

    Office of Scientific and Technical Information (OSTI)

    Channeling through Bent Crystals Stephanie Mack Office of Science, Science Undergraduate Laboratory Internship (SULI) University of Ottawa SLAC National Accelerator Laboratory...

  15. STATE OF CALIFORNIA - THE RESOURCES AGENCY

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Grant Mack, Executive Fellow to Chair Robert B. Weisenmiller, and Brian Turner, Deputy ... Attendees Name Affiliation Robert B. Weisenmiller Chair, California Energy Commission ...

  16. Teleseismic-Seismic Monitoring At Dixie Valley Geothermal Area...

    Open Energy Info (EERE)

    H. Ibser, Jennifer Lewicki, B. Mack. Kennedy, Michael Swyer (2013) Egs Exploration Methodology Project Using the Dixie Valley Geothermal System, Nevada, Status Update Ileana M....

  17. Numerical Modeling At Dixie Valley Geothermal Area (Iovenitti...

    Open Energy Info (EERE)

    H. Ibser, Jennifer Lewicki, B. Mack. Kennedy, Michael Swyer (2013) Egs Exploration Methodology Project Using the Dixie Valley Geothermal System, Nevada, Status Update Christoph...

  18. Magnetotellurics At Dixie Valley Geothermal Area (Iovenitti,...

    Open Energy Info (EERE)

    H. Ibser, Jennifer Lewicki, B. Mack. Kennedy, Michael Swyer (2013) Egs Exploration Methodology Project Using the Dixie Valley Geothermal System, Nevada, Status Update Phil...

  19. Gas Flux Sampling At Dixie Valley Geothermal Area (Iovenitti...

    Open Energy Info (EERE)

    H. Ibser, Jennifer Lewicki, B. Mack. Kennedy, Michael Swyer (2013) Egs Exploration Methodology Project Using the Dixie Valley Geothermal System, Nevada, Status Update Additional...

  20. Ground Magnetics At Dixie Valley Geothermal Area (Iovenitti,...

    Open Energy Info (EERE)

    H. Ibser, Jennifer Lewicki, B. Mack. Kennedy, Michael Swyer (2013) Egs Exploration Methodology Project Using the Dixie Valley Geothermal System, Nevada, Status Update Additional...

  1. Impacts of rising air temperatures and emissions mitigation on...

    Office of Scientific and Technical Information (OSTI)

    ; Colley, Michelle ; Patel, Pralit L. ; Eom, Jiyong ; Kim, Son H. ; Kyle, G. Page ; Schultz, Peter ; Venkatesh, Boddu ; Haydel, Juanita ; Mack, Charlotte ; Creason, Jared ...

  2. PARTICLE ACCELERATORS; ACCELERATORS; BEAM DUMPS; BENDING; CHANNELING...

    Office of Scientific and Technical Information (OSTI)

    Channeling through Bent Crystals Mack, Stephanie; Ottawa U. SLAC 43 PARTICLE ACCELERATORS; ACCELERATORS; BEAM DUMPS; BENDING; CHANNELING; CRYSTAL LATTICES; DETECTION; FORTRAN;...

  3. TITLE AUTHORS SUBJECT SUBJECT RELATED DESCRIPTION PUBLISHER AVAILABILI...

    Office of Scientific and Technical Information (OSTI)

    Channeling through Bent Crystals Mack Stephanie Ottawa U SLAC PARTICLE ACCELERATORS ACCELERATORS BEAM DUMPS BENDING CHANNELING CRYSTAL LATTICES DETECTION FORTRAN METERS MULTIPLE...

  4. Microsoft Word - S08266_FER_WetlandMititgationMonitorReport

    Office of Legacy Management (LM)

    Methods Ohio EPA provides detailed guidance with respect to monitoring methods and data analysis for evaluation of performance standards (Mack et al. 2004). As Table 3 shows, the...

  5. STEAB Teleconference Minutes May 2007

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Executive Director, ASERTTI, Virginia - Steve Vincent, Avista Utilities, Oregon - Alexander Mack, Manager, State Energy Program, Florida Energy Office - Peter Johnston, Manager, ...

  6. STEAB Teleconference Minutes January 2007

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Development, Arizona Public Service - Alexander Mack, Manager, State Energy Program, ... Natalie Alexander is your point person in this regard, and will assist you with scheduling ...

  7. STEAB Teleconference Minutes September 2007

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Vincent, Avista Utilities, Oregon - Alexander Mack, Manager, State Energy Program, ... Natalie Alexander (TMS, Inc.), to book your flights to and from the D.C. area. ...

  8. Advance Patent Waiver W(A)2005-042

    Broader source: Energy.gov [DOE]

    This is a request by MACK TRUCK for a DOE waiver of domestic and foreign patent rights under agreement DE-FC26-05NT42417.

  9. Advance Patent Waiver W(A)2005-041

    Broader source: Energy.gov [DOE]

    This is a request by MACK TRUCK for a DOE waiver of domestic and foreign patent rights under agreement DE-FC26-05NT42421.

  10. Search for: All records | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    ... Center, Casper, WY (United States) S. M. Stoller (United States) SLAC National ... Lei, Ming (1) Mack, Andrew (1) Mochrie, Simon (1) Regan, Lynne (1) Sachpatzidis, Aristidis ...

  11. Test of ''Crab-Waist'' Collisions at the DA{Phi}NE {Phi} Factory

    SciTech Connect (OSTI)

    Zobov, M.; Alesini, D.; Biagini, M. E.; Biscari, C.; Bocci, A.; Boni, R.; Boscolo, M.; Bossi, F.; Buonomo, B.; Clozza, A.; Delle Monache, G. O.; Demma, T.; Di Pasquale, E.; Di Pirro, G.; Drago, A.; Gallo, A.; Ghigo, A.; Guiducci, S.; Ligi, C.; Marcellini, F.

    2010-04-30

    The electron-positron collider DA{Phi}NE, the Italian {Phi} factory, has been recently upgraded in order to implement an innovative collision scheme based on large crossing angle, small beam sizes at the crossing point, and compensation of beam-beam interaction by means of sextupole pairs creating a ''crab-waist'' configuration in the interaction region. Experimental tests of the novel scheme exhibited an increase by a factor of 3 in the peak luminosity of the collider with respect to the performances reached before the upgrade. In this Letter we present the new collision scheme, discuss its advantages, describe the hardware modifications realized for the upgrade, and report the results of the experimental tests carried out during commissioning of the machine in the new configuration and standard operation for the users.

  12. NMR Analysis of Methyl Groups at 100-500 kDa: Model Systems and Arp2/3 Complex

    SciTech Connect (OSTI)

    Kreishman-Deitrick, Mara; Egile, Coumaran; Hoyt, David W.; Ford, Joseph J.; Rong, Li; Rosen, Michael K.

    2003-07-01

    Large macromolecular machines are among the most important and challenging targets for structural and mechanistic analyses. Consequently, there is great interest in development of NMR methods for the study of multicomponent systems in the 50-500 kDa range. Biochemical methods also must be developed in concert to produce such systems in selectively labeled form. Here, we present 1H/13C-HSQC spectra of protonated methyl groups in a model system that mimics molecular weights up to ~560 kDa. Signals from side chain methyl groups of Ile, Leu, and Val residues are clearly detectable at correlation times up to ~330 ns. We have also developed a biochemical procedure to produce the 240 kDa, heteroheptameric Arp2/3 actin nucleation complex selectively labeled at one subunit and obtained 1H/13C-HSQC spectra of this assembly. Sensitivity in spectra of both the Arp2/3 complex and the model system indicate that methyl groups will be useful sources of information in nonsymmetric systems with molecular weights greater than 600 kDa at concentrations less than 100 μM. Methyl analyses will complement TROSY and CRINEPT analyses of amides in NMR studies of structure and molecular interactions of extremely large macromolecules and assemblies.

  13. CX-009709: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Wood Pole Replacements Along the Drummond-Macks Inn, Macks Inn-Madison, and Swan Valley-Teton 115-kilovolt Transmission Line Rights-of-Way CX(s) Applied: B1.3 Date: 11/29/2012 Location(s): Idaho, Idaho Offices(s): Bonneville Power Administration

  14. CX-007991: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Wood Pole Replacements Along the Drummond-Macks Inn, Macks Inn-Madison, and Targhee Tap 115-Kilovolt Transmission Line Right-of-ways CX(s) Applied: B1.3 Date: 02/10/2012 Location(s): Idaho Offices(s): Bonneville Power Administration

  15. The 21.5-kDa isoform of myelin basic protein has a non-traditional PY-nuclear-localization signal

    SciTech Connect (OSTI)

    Smith, Graham S.T.; Seymour, Lauren V.; Boggs, Joan M.; Harauz, George

    2012-06-15

    Highlights: Black-Right-Pointing-Pointer Full-length 21.5-kDa MBP isoform is translocated to the nucleus. Black-Right-Pointing-Pointer We hypothesized that the exon-II-encoded sequence contained the NLS. Black-Right-Pointing-Pointer We mutated this sequence in RFP-tagged constructs and transfected N19-cells. Black-Right-Pointing-Pointer Abolition of two key positively-charged residues resulted in loss of nuclear-trafficking. Black-Right-Pointing-Pointer The 21.5-kDa isoform of classic MBP contains a non-traditional PY-NLS. -- Abstract: The predominant 18.5-kDa classic myelin basic protein (MBP) is mainly responsible for compaction of the myelin sheath in the central nervous system, but is multifunctional, having numerous interactions with Ca{sup 2+}-calmodulin, actin, tubulin, and SH3-domains, and can tether these proteins to a lipid membrane in vitro. The full-length 21.5-kDa MBP isoform has an additional 26 residues encoded by exon-II of the classic gene, which causes it to be trafficked to the nucleus of oligodendrocytes (OLGs). We have performed site-directed mutagenesis of selected residues within this segment in red fluorescent protein (RFP)-tagged constructs, which were then transfected into the immortalized N19-OLG cell line to view protein localization using epifluorescence microscopy. We found that 21.5-kDa MBP contains two non-traditional PY-nuclear-localization signals, and that arginine and lysine residues within these motifs were involved in subcellular trafficking of this protein to the nucleus, where it may have functional roles during myelinogenesis.

  16. Increases in 3He/4He in Fumarolic Gas Associated with the 1989...

    Open Energy Info (EERE)

    Beneath Mammoth Mountain, California Citation Michael L. Sorey,B. Mack Kennedy,William C. Evans,Christopher D. Farrar. 1990. Increases in 3He4He in Fumarolic Gas Associated with...

  17. Geology of Southwestern New Mexico | Open Energy Information

    Open Energy Info (EERE)

    to library Conference Paper: Geology of Southwestern New Mexico Authors R.E. Clemons and G.H. Mack Conference 39th Field Conference; ConferencePlace"ConferencePlace"...

  18. Personal Property Management Program Brochure

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ...nnie.Anderson@ch.doe.gov EM-Savannah River-Tim Armstrong Timothy.armstrong@srs.gov ... 202-287-1596 tyrone.ware@hq.doe.gov Mack Burton, Fleet Management Lead ...

  19. Fluid Inclusion Gas Compositions From An Active Magmatic-Hydrothermal...

    Open Energy Info (EERE)

    remained closed to the diffusion of He and H2 since their formation. Authors Joseph N. Moore, David I. Norman and B. Mack Kennedy Published Journal Chemical Geology, 2001 DOI...

  20. "Title","Creator/Author","Publication Date","OSTI Identifier...

    Office of Scientific and Technical Information (OSTI)

    Channeling through Bent Crystals","Mack, Stephanie; Ottawa U. SLAC","2012-09-07T04:00:00Z",1050214,"10.21721050214","SLAC-TN-12-026","AC02-76SF00515","TRN: US1204684","Technical...

  1. SEP News | Department of Energy

    Energy Savers [EERE]

    (EnMS) that meets the requirements of ISO 50001 and ... Engine Plant (RMEP), a natural gas and diesel engine ... General Dynamics, Land O'Lakes, and Mack Trucks ...

  2. Tritium Instrument Demonstration Station (TIDS)

    Office of Environmental Management (EM)

    - 488 nm; 200 mW max power RoMack Inc. 20' fiber optic (6 around 1) on " VCR fitting Kaiser Optical Holospec- f1.8 I VIS w 488 grating Andor DV-420-OE CCD detector Detection...

  3. Utah_cm_smith

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Cindy and Mack Smith Site - Utah Wind Anemometer Loan Program Latitude: N. 37 deg. 44.034' Longitude: W. 109 deg. 17.28' Elevation: 6762' Placed in service: November 21, 2002...

  4. Help - DOE Directives, Delegations, and Requirements

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    (202) 287-5641 Christopher Tirado, (202) 586-4967 Rachel Mack, (202) 287-6804 Delegation Program Management Gail Cephas,(202) 586-1049 Web Site Administration Technical Support...

  5. DOE Directives, Delegations, and Requirements

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    (202) 287-5641 Christopher Tirado, (202) 586-4967 Rachel Mack, (202) 287-6804 Delegation Program Management Gail Cephas,(202) 586-1049 Web Site Administration Technical Support...

  6. Study of the K{sub stop}{sup -}A{yields}{Sigma}{sup {+-}}{pi}{sup {+-}}A' reaction at DA{Phi}NE

    SciTech Connect (OSTI)

    Agnello, M.; Benussi, L.; Bertani, M.; Fabbri, F. L.; Gianotti, P.; Lucherini, V.; Bhang, H. C.; Bonomi, G.; Moia, F.; Zenoni, A.; Botta, E.; Bressani, T.; Bufalino, S.; Busso, L.; Calvo, D.; De Mori, F.; Feliciello, A.; Filippi, A.; Marcello, S.; Wheadon, R.

    2010-12-28

    This work describes an experimental study of the K{sub stop}{sup -}A{yields}{pi}{sup {+-}}{Sigma}{sup {+-}}A' reaction performed with the FINUDA spectrometer at the DA{Phi}NE {phi}-factory. The reaction is studied via the detection of {pi}{sup +}{pi}{sup -}n events on {sup 6,7}Li, {sup 9}Be, {sup 13}C and {sup 16}O.

  7. Data:B485777c-c4fb-42b3-8d2a-3da95f7c7c10 | Open Energy Information

    Open Energy Info (EERE)

    a-3da95f7c7c10 No revision has been approved for this page. It is currently under review by our subject matter experts. Jump to: navigation, search Loading... 1. Basic Information...

  8. DA326576 (2 pages)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

  9. Crystallization and X-ray data analysis of the 10 kDa C-terminal lid subdomain from Caenorhabditis elegans Hsp70

    SciTech Connect (OSTI)

    Worrall, Liam; Walkinshaw, Malcolm D., E-mail: m.walkinshaw@ed.ac.uk [School of Biological Sciences, University of Edinburgh, The Kings Buildings, Mayfield Road, Edinburgh EH9 3JR,Scotland (United Kingdom)

    2006-09-01

    Crystals of the C-terminal 10 kDa lid subdomain from the C. elegans chaperone Hsp70 have been obtained that diffract X-rays to ?3.5 and belong to space group I2{sub 1}2{sub 1}2{sub 1}. Analysis of X-ray data and initial heavy-atom phasing reveals 24 monomers in the asymmetric unit related by 432 non-crystallographic symmetry. Hsp70 is an important molecular chaperone involved in the regulation of protein folding. Crystals of the C-terminal 10 kDa helical lid domain (residues 542640) from a Caenorhabditis elegans Hsp70 homologue have been produced that diffract X-rays to ?3.4 . Crystals belong to space group I2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = b = 197, c = 200 . The Matthews coefficient, self-rotation function and Patterson map indicate 24 monomers in the asymmetric unit, showing non-crystallographic 432 symmetry. Molecular-replacement studies using the corresponding domain from rat, the only eukaryotic homologue with a known structure, failed and a mercury derivative was obtained. Preliminary MAD phasing using SHELXD and SHARP for location and refinement of the heavy-atom substructure and SOLOMON for density modification produced interpretable maps with a clear proteinsolvent boundary. Further density-modification, model-building and refinement are currently under way.

  10. Broken Scale Invariance and Anomalous Dimensions

    DOE R&D Accomplishments [OSTI]

    Wilson, K. G.

    1970-05-01

    Mack and Kastrup have proposed that broken scale invariance is a symmetry of strong interactions. There is evidence from the Thirring model and perturbation theory that the dimensions of fields defined by scale transformations will be changed by the interaction from their canonical values. We review these ideas and their consequences for strong interactions.

  11. Pennsylvania Manufacturing Plants Recognized as Leaders in Energy Management

    Broader source: Energy.gov [DOE]

    On April 7, 2015, the U.S. Department of Energy (DOE) recognized four Pennsylvania manufacturing plants owned by Curtiss-Wright, General Dynamics, Land O’Lakes, and Mack Trucks for their leadership in energy management by earning certification to Superior Energy Performance®.

  12. National Laboratory Contacts | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Laboratory Contacts National Laboratory Contacts The Geothermal Technologies Office works closely with several DOE national laboratories in managing and contributing to research and development projects. Below are the primary contacts at these laboratories. Laboratory Name Idaho National Laboratory Greg Mines, Lead Manager Lawrence Berkeley National Laboratory Mack Kennedy, Lead Scientist Lawrence Livermore National Laboratory Jeff Roberts, Lead Scientist National Renewable Energy Laboratory Tom

  13. National Nuclear Security Administration Los Alamos Site Office

    Office of Environmental Management (EM)

    Laboratory Contacts National Laboratory Contacts The Geothermal Technologies Office works closely with several DOE national laboratories in managing and contributing to research and development projects. Below are the primary contacts at these laboratories. Laboratory Name Idaho National Laboratory Greg Mines, Lead Manager Lawrence Berkeley National Laboratory Mack Kennedy, Lead Scientist Lawrence Livermore National Laboratory Jeff Roberts, Lead Scientist National Renewable Energy Laboratory Tom

  14. Combined Use of Residual Dipolar Couplings and Solution X-ray Scattering To Rapidly Probe Rigid-Body Conformational Transitions in a Non-phosphorylatable Active-Site Mutant of the 128 kDa Enzyme I Dimer

    SciTech Connect (OSTI)

    Takayama, Yuki; Schwieters, Charles D.; Grishaev, Alexander; Ghirlando, Rodolfo; Clore, G. Marius (NIH)

    2012-10-23

    The first component of the bacterial phosphotransferase system, enzyme I (EI), is a multidomain 128 kDa dimer that undergoes large rigid-body conformational transitions during the course of its catalytic cycle. Here we investigate the solution structure of a non-phosphorylatable active-site mutant in which the active-site histidine is substituted by glutamine. We show that perturbations in the relative orientations and positions of the domains and subdomains can be rapidly and reliably determined by conjoined rigid-body/torsion angle/Cartesian simulated annealing calculations driven by orientational restraints from residual dipolar couplings and shape and translation information afforded by small- and wide-angle X-ray scattering. Although histidine and glutamine are isosteric, the conformational space available to a Gln side chain is larger than that for the imidazole ring of His. An additional hydrogen bond between the side chain of Gln189 located on the EIN{sup {alpha}/{beta}} subdomain and an aspartate (Asp129) on the EIN{sup {alpha}} subdomain results in a small ({approx}9{sup o}) reorientation of the EIN{sup {alpha}} and EIN{sup {alpha}/{beta}} subdomains that is in turn propagated to a larger reorientation ({approx}26{sup o}) of the EIN domain relative to the EIC dimerization domain, illustrating the positional sensitivity of the EIN domain and its constituent subdomains to small structural perturbations.

  15. MEMORANDUM TO: FSLE DA-C

    Office of Legacy Management (LM)

    Refined Source Material q Residue ci Natural Radioactive Material from Non-Nuclear AC 0 Man-Made f G atfier-------- I -----....

  16. Jayme da Costa | Open Energy Information

    Open Energy Info (EERE)

    energy Product: Electrical goods manufacturer that is developing and building wind, solar and hydro projects in Portugal and wind projects in Spain. Coordinates: 40.875332,...

  17. PLEAEERUSH ANALYTICAL DA-~-A SHEET

    Office of Legacy Management (LM)

    mDeriod;.02; 151 .3 while furnace was charged with UOT- and carbon, and under heat. 7393 GA Continuation of 7392 I I 7394 GA Continuation of 7393 -I- ----J ---- - ...

  18. 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Flow cytometry aids basic cell biology research and drug discovery April 3, 2012 Examining cells has never been clearer with LANL's use of acoustic waves Flow cytometry dates back to a cell sorter built by Mack Fulwyler at Los Alamos in 1965. Several additional contributions to the technology have been made at the National Flow Cytometry Resource of Los Alamos National Laboratory (LANL). Flow cytometry allows scientists to quantify and examine cells by passing them through a laser-based

  19. Search for: All records | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    Mack, Stephanie" Name Name ORCID Search Authors Type: All Book/Monograph Conference/Event Journal Article Miscellaneous Patent Program Document Software Manual Technical Report Thesis/Dissertation Subject: Identifier Numbers: Site: All Alaska Power Administration, Juneau, Alaska (United States) Albany Research Center (ARC), Albany, OR (United States) Albuquerque Complex - NNSA Albuquerque Operations Office, Albuquerque, NM (United States) Amarillo National Resource Center for Plutonium,

  20. U.S. Expands Energy Cooperation with Sweden | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Expands Energy Cooperation with Sweden U.S. Expands Energy Cooperation with Sweden June 28, 2007 - 2:08pm Addthis DOE to Work with Volvo AB/Mack Trucks on Advanced Vehicle R&D STOCKHOLM, SWEDEN - U.S. Department of Energy's (DOE) Assistant Secretary for Energy Efficiency and Renewable Energy Alexander Karsner and Swedish Deputy Prime Minister and Minister of Enterprise and Energy Maud Olofsson today signed an implementing agreement to further expand cooperation on renewable energy and

  1. Zhang-Y

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Surface Solar Radiation Budget: Corrections for Ice Clouds Y. Zhang and Z. Li EMS, Canada Centre for Remote Sensing Ottawa, K1A 0Y7, Canada A. Macke Institut fuer Meereskunde 24105 Kiel, Germany Introduction Surface solar radiative budget (SSRB) is modulated primarily by clouds whose effects must be taken into account carefully in estimating it from satellite measured upward solar radiative flux at top of the atmosphere (TOA). To avoid time-consuming calculations in operational retrieval of SSRB

  2. NREL Document Profiles Natural Gas Fueling, Fleet Operation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Document Profiles Natural Gas Fueling, Fleet Operation Media may contact: George Douglas, 303-275-4096 email: George Douglas Steve Ginter, Mack, 610-709-3259 Golden, Colo., June 7, 2000 - A unique and successful natural gas fueling and fleet operation involving trash haulers is discussed in a recent document issued by the U.S. Department of Energy's National Renewable Energy Laboratory (NREL). The NREL document, Waste Management's LNG Truck Fleet Start-Up Experience, offers solid evidence that

  3. STEAB Meeting Minutes August 2007

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    State Energy Advisory Board Meeting August 14-16, 2007 Berkeley, CA. A Visit to the Lawrence Berkeley National Laboratory BOARD MEMBERS PRESENT Chris Benson (Chairman) (AR), Patricia Sobrero (Vice-Chair) (VA), Elliot Jacobson (Secretary) (MA), Robert Hoppie (ID), Janet Streff (MN), Duane Hauck (ND), Peter Johnston (AZ), John Davies (KY), William "Dub" Taylor (TX), Alexander Mack (FL), Jim Ploger (KS), Jim Nolan (MT), Susan Brown (WI), Paul Gutierrez (NM), and Steven Vincent (OR).

  4. mitchell(1)-98.pdf

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    9 Testing a Cirrus Radiation Scheme with In Situ Microphysical and Radiometric Measurements from a Tropical Cirrus Anvil D. L. Mitchell and D. Ivanova Atmospheric Sciences Center Desert Research Institute Reno, Nevada G. M. McFarquhar National Center for Atmospheric Research Boulder, Colorado A. Macke Universitaet zu Kiel Kiel, Germany Experimental Design and Objective The only successful microphysics/radiation experiment to date in tropical anvil cirrus was the 4 April 1993 mission during the

  5. Cloud Microphysical and Radiative Properties Derived from MODIS, VIRS, AVHRR, and GMS Data Over the Tropical Western Pacific

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Microphysical and Radiative Properties Derived from MODIS, VIRS, AVHRR, and GMS Data Over the Tropical Western Pacific G. D. Nowicki, M. L. Nordeen, P. W. Heck, D. R. Doelling, and M. M. Khaiyer Analytical Services and Materials, Inc. Hampton, Virginia P. Minnis National Aeronautics and Space Administration Atmospheric Sciences Division Langley Research Center Hampton, Virginia S. Sun-Mack Science Applications International Corporation Hampton, Virginia Introduction Utilization of the

  6. REAC/TS Management of Radiation Accidents 2015-16 Course Brochure

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Adayabalam S. Balajee, PhD Technical Director, REAC/TS Cytogenetic Biodosimetry Laboratory (CBL) Maria Escalona, BS MS Lead Biologist / Cytogenetics-Biodosimetry Cytogenetic Biodosimetry Laboratory (CBL) Becky Murdock, AS REAC/TS Education Coordinator Glenda Gross REAC/TS Administrative Assistant Amanda Hughes REAC/TS Travel / Property Coordinator Gail Mack-Bramlette REAC/TS Course Registrar Name: Last First Middle Initial Degree/Certification Home Address City State Zip Code Country Home Area

  7. ARM07Chang_poster.ppt

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Retrievals and Comparisons of Various MODIS-Spectrum Inferred Water Cloud Droplet Effective Radii Fu-Lung Chang @ , Patrick Minnis # , Bing Lin # , Sunny Sun-Mack & , Mandana Khaiyer & @National Institute of Aerospace #NASA Langley Research Center &Science System Applications Inc The 17th ARM Science Team Meeting, Monterey, California, March 26-30, 2007 Contact: Dr. Fu-Lung Chang, National Institute of Aerospace (NIA) Email: f.chang@larc.nasa.gov  How different are the retrievals

  8. Super-Poissonian Statistics of Photon Emission from Single CdSe-CdS

    Office of Scientific and Technical Information (OSTI)

    Core-Shell Nanocrystals Coupled to Metal Nanostructures (Journal Article) | SciTech Connect Super-Poissonian Statistics of Photon Emission from Single CdSe-CdS Core-Shell Nanocrystals Coupled to Metal Nanostructures Citation Details In-Document Search Title: Super-Poissonian Statistics of Photon Emission from Single CdSe-CdS Core-Shell Nanocrystals Coupled to Metal Nanostructures Authors: Park, Young-Shin ; Ghosh, Yagnaseni ; Chen, Yongfen ; Piryatinski, Andrei ; Xu, Ping ; Mack, Nathan H. ;

  9. X:\ARM_19~1\P193-223.WPD

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    eff µm r eff µm 10 3 ( i ) i w µ 0 µ 180 Session Papers 211 Figure 1. Phase functions of fractal ice particles (solid line) and ISCCP water droplets (dotted line) at 0.63 µm. Effect of Particle Nonsphericity on Bidirectional Reflectance of Cirrus Clouds M. I. Mishchenko, W. B. Rossow, A. Macke, and A. A. Lacis National Aeronautics and Space Administration Goddard Institute for Space Studies New York, New York Previous analyses of the First International Satellite Cloud Climatology Project

  10. Research Highlight

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Validation of CERES-MODIS Cloud Retrievals Using the Azores Data Download a printable PDF Submitter: Dong, X., University of North Dakota Area of Research: Cloud Processes Working Group(s): Cloud Life Cycle Journal Reference: Xi B, P Minnis, and S Sun-Mack. 2014. "Comparison of marine boundary layer cloud properties from CERES-MODIS edition 4 and DOE ARM AMF measurements at the Azores." Journal of Geophysical Research - Atmospheres, 119, doi:10.1002/2014JD021813. Figure 1. The ARM

  11. C. R. Yost

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    R. Yost 1 , P. Minnis 2 , J. K. Ayers 1 , R. Palikonda 1 , D. Spangenberg 1 , S. Sun-Mack 1 , Y. Chen 1 1 Science Systems & Applications, Inc. Hampton, VA 2 NASA Langley Research Center Hampton, VA Introduction Accurate cloud top heights are necessary to compute TOA fluxes in climate models, but passive IR-based satellite retrievals and ground measurements tend to underestimate the top altitude of ice-phase clouds (e.g., anvils). * IR retrievals underestimate cloud top heights by 1-2 km

  12. Research Highlight

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Validation of CERES-MODIS Cloud Properties Using ARM Data Submitter: Dong, X., University of North Dakota Area of Research: Cloud Distributions/Characterizations Working Group(s): Cloud Properties Journal Reference: Dong, X., P. Minnis, B. Xi, S. Sun-Mack, and Y. Chen, 2007: Validation of CERES-MODIS stratus cloud properties using ground-based measurements at the DOE ARM SGP site. Accepted by J. Geophys. Res. Wielicki, B. A. and Co-authors (2000), CERES Validation Plan Overview, Release 4,

  13. Heavy-Duty Waste Hauler with Chemically Correct Natural Gas Engine Diluted with EGR and Using a Three-Way Catalyst: Final Report, 24 February 2004 -- 23 February 2006

    Alternative Fuels and Advanced Vehicles Data Center [Office of Energy Efficiency and Renewable Energy (EERE)]

    Heavy-Duty Waste Hauler with Chemically Correct Natural Gas Engine Diluted with EGR and Using a Three-Way Catalyst Final Report February 24, 2004 - February 23, 2006 T. Reppert Mack Trucks, Inc. Allentown, Pennsylvania J. Chiu Southwest Research Institute San Antonio, Texas Subcontract Report NREL/SR-540-38222 September 2005 Heavy-Duty Waste Hauler with Chemically Correct Natural Gas Engine Diluted with EGR and Using a Three-Way Catalyst Final Report February 24, 2004 - February 23, 2006 T.

  14. Effects of Biomass Fuels on Engine & System Out Emissions for Short Term

    Broader source: Energy.gov (indexed) [DOE]

    Endurance | Department of Energy Results of an investigation into effects of biofuels on engine- and system-out emissions, specifically US 2010 EPA exhaust after-treatment system from Mack Trucks PDF icon deer11_barnum.pdf More Documents & Publications High Fuel Economy Heavy-Duty Truck Engine A European Perspective of EURO 5/U.S. 07 Heavy-Duty Engine Technologies and Their Related Consequences Lowest Engine-Out Emissions as the Key to the Future of the Heavy-Duty Diesel Engine: New

  15. Domingos da Silva Teixeira SA DST | Open Energy Information

    Open Energy Info (EERE)

    Portugal Zip: 4711 911 Sector: Solar, Wind energy Product: A group that is involved in civil construction and public works as well as project development in the wind and solar...

  16. MHK Projects/Figueira da Foz Portugal | Open Energy Information

    Open Energy Info (EERE)

    a commercial power project site in Figueira de Foz, Portugal to build a 100 MW offshore wave energy plant. Initially, a 2 MW demonstration plant is planned followed by the...

  17. RUMINATIONS ON NDA MEASUREMENT UNCERTAINTY COMPARED TO DA UNCERTAINTY

    SciTech Connect (OSTI)

    Salaymeh, S.; Ashley, W.; Jeffcoat, R.

    2010-06-17

    It is difficult to overestimate the importance that physical measurements performed with nondestructive assay instruments play throughout the nuclear fuel cycle. They underpin decision making in many areas and support: criticality safety, radiation protection, process control, safeguards, facility compliance, and waste measurements. No physical measurement is complete or indeed meaningful, without a defensible and appropriate accompanying statement of uncertainties and how they combine to define the confidence in the results. The uncertainty budget should also be broken down in sufficient detail suitable for subsequent uses to which the nondestructive assay (NDA) results will be applied. Creating an uncertainty budget and estimating the total measurement uncertainty can often be an involved process, especially for non routine situations. This is because data interpretation often involves complex algorithms and logic combined in a highly intertwined way. The methods often call on a multitude of input data subject to human oversight. These characteristics can be confusing and pose a barrier to developing and understanding between experts and data consumers. ASTM subcommittee C26-10 recognized this problem in the context of how to summarize and express precision and bias performance across the range of standards and guides it maintains. In order to create a unified approach consistent with modern practice and embracing the continuous improvement philosophy a consensus arose to prepare a procedure covering the estimation and reporting of uncertainties in non destructive assay of nuclear materials. This paper outlines the needs analysis, objectives and on-going development efforts. In addition to emphasizing some of the unique challenges and opportunities facing the NDA community we hope this article will encourage dialog and sharing of best practice and furthermore motivate developers to revisit the treatment of measurement uncertainty.

  18. CoDA 2016, the Conference on Data Analysis

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    program explores six themes: Really Expensive Data Power Grid Data Multisource Data Cybersecurity Subsurface Modeling Data Analysis at Exascale We welcome posters on these and...

  19. fdm3da_allocV1.0

    Energy Science and Technology Software Center (OSTI)

    2002-01-23

    FORTRAN90 software computes synthetic induction log responses in fully 3D anistropic geoelectric media.

  20. fdm3da_parV1.0

    Energy Science and Technology Software Center (OSTI)

    2002-01-23

    FORTRAN90 software computes synthetic induction log responses in fully 3D anistropic geoelectric media.

  1. fdm3daV1.0

    Energy Science and Technology Software Center (OSTI)

    2001-06-06

    FORTRAN90 software computes synthetic induction log responses in fully 3D anistropic geoelectric media.

  2. Data:Dba74d36-87bb-4a6d-a55b-67d341da9431 | Open Energy Information

    Open Energy Info (EERE)

    contentdampacificpowerdocAboutUsRatesRegulationWashingtonApprovedTariffsWAPriceSummary.pdf Source Parent: https:www.pacificpower.netaboutrrwri.html Comments...

  3. TO. TO. , W. B; Harris, Chief, Industrial Hygiene'Branch DA ...

    Office of Legacy Management (LM)

    ... Hartm ann and M ro Nagy t: work will take from .three to sti m onths to com plete, cc: E. J, Kehoe, Chief Fire & Accident Prevention Branch' . I is tb .tk, hai July 5, 1950 crium ...

  4. Numerical Analysis of Parasitic Crossing Compensation with Wires in DA$\\Phi$NE

    SciTech Connect (OSTI)

    Valishev, A.; Shatilov, D.; Milardi, C.; Zobov, M.

    2015-06-24

    Current-bearing wire compensators were successfully used in the 2005-2006 run of the DAΦNE collider to mitigate the detrimental effects of parasitic beam-beam interactions. A marked improvement of the positron beam lifetime was observed in machine operation with the KLOE detector. In view of the possible application of wire beam-beam compensators for the High Luminosity LHC upgrade, we revisit the DAΦNE experiments. We use an improved model of the accelerator with the goal to validate the modern simulation tools and provide valuable input for the LHC upgrade project.

  5. DOE-EIS-0222-SA-01_-_Rev_0_-_[DA07313710].pdf

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

  6. Simulation of Crab Waist Collisions In DA$\\Phi$NE With KLOE-2 Interaction Region

    SciTech Connect (OSTI)

    Zobov, M.; Drago, A.; Gallo, A.; Milardi, C.; Shatilov, D.; Valishev, A.

    2015-06-24

    After the successful completion of the SIDDHARTA experiment run with crab waist collisions, the electron-positron collider DAΦNE has started routine operations for the KLOE-2 detector. The new interaction region also exploits the crab waist collision scheme, but features certain complications including the experimental detector solenoid, compensating anti-solenoids, and tilted quadrupole magnets. We have performed simulations of the beam-beam collisions in the collider taking into account the real DAΦNE nonlinear lattice. In particular, we have evaluated the effect of crab waist sextupoles and beam-beam interactions on the DAΦNE dynamical aperture and energy acceptance, and estimated the luminosity that can be potentially achieved with and without crab waist sextupoles in the present working conditions. A numerical analysis has been performed in order to propose possible steps for further luminosity increase in DAΦNE such as a better working point choice, crab sextupole strength optimization, correction of the phase advance between the sextupoles and the interaction region. The proposed change of the e- ring working point was implemented and resulted in a significant performance increase.

  7. L3:MPO.CRUD.P6.01 D.A. Andersson, C. R. Stanek LANL December...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of nano- and microstructure on the electronic and magnetic properties of NiFe 2 O 4 4-7 . ... due to its neutron absorbing properties, shift the power distribution along the rod axis ...

  8. Truck Thermoacoustic Generator and Chiller

    SciTech Connect (OSTI)

    Robert Keolian

    2011-03-31

    This Final Report describes the accomplishments of the US Department of Energy (DOE) cooperative agreement project DE-FC26-04NT42113 - Truck Thermoacoustic Generator and Chiller - whose goal is to design, fabricate and test a thermoacoustic piezoelectric generator and chiller system for use on over-the-road heavy-duty-diesel trucks, driven alternatively by the waste heat of the main diesel engine exhaust or by a burner integrated into the thermoacoustic system. The thermoacoustic system would utilize engine exhaust waste heat to generate electricity and cab air conditioning, and would also function as an auxiliary power unit (APU) for idle reduction. The unit was to be tested in Volvo engine performance and endurance test cells and then integrated onto a Class 8 over-the-road heavy-duty-diesel truck for further testing on the road. The project has been a collaboration of The Pennsylvania State University Applied Research Laboratory, Los Alamos National Laboratory, Clean Power Resources Inc., and Volvo Powertrain (Mack Trucks Inc.). Cost share funding was provided by Applied Research Laboratory, and by Clean Power Resources Inc via its grant from Innovation Works - funding that was derived from the Commonwealth of Pennsylvania. Los Alamos received its funding separately through DOE Field Work Proposal 04EE09.

  9. Psychology and the prevention of nuclear war

    SciTech Connect (OSTI)

    White, R.K.

    1986-01-01

    This book is about our ways of thinking and about how they need to be and can be changed. It is not about the ''unparalleled catastrophe.'' By now many of us know much about that, and unless we an see clear, acceptable, and practical ways to prevent it, our minds recoil from the whole horrible subject. Therefore, the book is about the prevention of nuclear war and nothing else. At least, that is its purpose. Yet its method is primarily descriptive and analytical rather than action-oriented. It explores from different perspectives the possible causes of a world war that could be at the outset, or become, nuclear, with a special focus on the often-neglected psychological aspects of those causes. It is diagnosis more than prescription. In fact, it might be described as a many-sided effort to understand the nature and roots of the ''madness'' of our present drift toward a great war that each side is urgently-desperately-anxious to avoid. In so doing it draws on some of the insights of psychiatry (from the psychiatrists Robert Jay Lifton, John E. Mack, Jerome D. Frank, and Erich Fromm), as well as on the three disciplines that provide the chief foundation for the book: history, political science, and social psychology.

  10. Sampling Plan: Engineering Sampling Plan to Identify Areas for Remediation in the Southeast Drainage (Vicinity Properties DA-4 and DOC-7) DOE/OR/21548-582

    Office of Legacy Management (LM)

  11. Southeast Drainage Closeout Report Vicinity Properties DA4 and MDC7. Revision 0 is dated September 1999. DOE/OR/21548-772. SE-100-105-1.

    Office of Legacy Management (LM)

  12. Data:C9125b18-da0a-4612-b0c6-3eb6421ac6c2 | Open Energy Information

    Open Energy Info (EERE)

    b0c6-3eb6421ac6c2 No revision has been approved for this page. It is currently under review by our subject matter experts. Jump to: navigation, search Loading... 1. Basic...

  13. Data:Ea824b4e-a3bf-4763-a763-da0599d9d760 | Open Energy Information

    Open Energy Info (EERE)

    under review by our subject matter experts. Jump to: navigation, search Loading... 1. Basic Information 2. Demand 3. Energy << Previous 1 2 3 Next >> Basic Information...

  14. Data:Ed681d16-9c5b-4ae3-a097-4da62829c05b | Open Energy Information

    Open Energy Info (EERE)

    under review by our subject matter experts. Jump to: navigation, search Loading... 1. Basic Information 2. Demand 3. Energy << Previous 1 2 3 Next >> Basic Information...

  15. fn{EE49F893-CA64-40D2-9A32-E9DA8936271E}EIMS+Content&dbwisle...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Errors and Omissions Process ID: PCS.062 Revision : 5 Revision Date: 04272012 Page: 1 of 2 Task Owner: * PA Project Associate * DS Design Supplier * SCR Sandia Contracting ...

  16. Very High Fuel Economy, Heavy Duty, Constant Speed, Truck Engine Optimized Via Unique Energy Recovery Turbines and Facilitated High Efficiency Continuously Variable Drivetrain

    SciTech Connect (OSTI)

    Bahman Habibzadeh

    2010-01-31

    The project began under a corporative agreement between Mack Trucks, Inc and the Department of Energy starting from September 1, 2005. The major objective of the four year project is to demonstrate a 10% efficiency gain by operating a Volvo 13 Litre heavy-duty diesel engine at a constant or narrow speed and coupled to a continuously variable transmission. The simulation work on the Constant Speed Engine started on October 1st. The initial simulations are aimed to give a basic engine model for the VTEC vehicle simulations. Compressor and turbine maps are based upon existing maps and/or qualified, realistic estimations. The reference engine is a MD 13 US07 475 Hp. Phase I was completed in May 2006 which determined that an increase in fuel efficiency for the engine of 10.5% over the OICA cycle, and 8.2% over a road cycle was possible. The net increase in fuel efficiency would be 5% when coupled to a CVT and operated over simulated highway conditions. In Phase II an economic analysis was performed on the engine with turbocompound (TC) and a Continuously Variable Transmission (CVT). The system was analyzed to determine the payback time needed for the added cost of the TC and CVT system. The analysis was performed by considering two different production scenarios of 10,000 and 60,000 units annually. The cost estimate includes the turbocharger, the turbocompound unit, the interstage duct diffuser and installation details, the modifications necessary on the engine and the CVT. Even with the cheapest fuel and the lowest improvement, the pay back time is only slightly more than 12 months. A gear train is necessary between the engine crankshaft and turbocompound unit. This is considered to be relatively straight forward with no design problems.

  17. Solco Europe Ltd | Open Energy Information

    Open Energy Info (EERE)

    Solco Europe Ltd Place: Sao Joao da Madeira, Portugal Zip: Sao Joao da Madeira Product: Joint venture to distribute Solco products within Portugal. References: Solco Europe...

  18. Categorical Exclusion 4567, MPLE Test Stand Replacement Project

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and Guidelines DA 14 - Approval of technical exchange arrangements DA 15 - International umbrella agreements for energy R&D Facility Operations DB 1.2 - Training exercises and...

  19. Destilaria PAL | Open Energy Information

    Open Energy Info (EERE)

    PAL Jump to: navigation, search Name: Destilaria PAL Place: Nazare da Mata, Brazil Zip: 55800-00 Product: Brazil based ethanol producer located in Nazare da Mata, Pernambuco....

  20. Ultra-low Sulfur Reduction Emission Control Device/Development of an On-board Fuel Sulfur Trap

    SciTech Connect (OSTI)

    Rohrbach, Ron; Barron, Ann

    2008-07-31

    Honeywell has completed working on a multiyear program to develop and demonstrate proof-of-concept for an 'on-vehicle' desulfurization fuel filter for both light duty and heavy-duty diesel engines. Integration of the filter into the vehicle fuel system will reduce the adverse effects sulfur has on post combustion emission control devices such as NOx adsorbers. The NOx adsorber may be required to meet the proposed new EPA Tier II and '2007-Rule' emission standards. The proposed filter concept is based on Honeywell's reactive filtration technology and experience in liquids handling and conditioning. A regeneration and recycling plan for the spent filters was also examined. We have chosen to develop and demonstrate this technology based on criteria set forth for a heavy duty CIDI engine system because it represents a more challenging set of conditions of service intervals and overall fuel usage over light duty systems. In the second phase of the program a light duty diesel engine test was also demonstrated. Further, technology developed under this proposal would also have application for the use of liquid based fuels for fuel cell power generation. The program consisted of four phases. Phase I focused on developing a concept design and analysis and resolution of technical barriers concerning removal of sulfur-containing species in low sulfur fuels. In Phase II concentrated on prototype filter design and preparation followed by qualification testing of this component in a fuel line application. Phase III studied life cycle and regeneration options for the spent filter. Phase IV focused on efficacy and benefits in the desulfation steps of a NOx adsorber on both a heavy and light duty engine. The project team included a number of partners, with Honeywell International as the prime contractor. The partners include an emission control technology developer (Honeywell International), a fuel technology developer (Marathon Ashland Petroleum), a catalyst technology developer (Johnson Matthey), a CIDI engine manufacturer (Navistar Inc. (formerly International Truck & Engine Corporation) and Mack Trucks Inc.), and filter recycler (American Wastes Industries).

  1. Test, Evaluation, and Demonstration of Practical Devices/Systems to Reduce Aerodynamic Drag of Tractor/Semitrailer Combination Unit Trucks

    SciTech Connect (OSTI)

    Scott Smith; Karla Younessi; Matt Markstaller; Dan Schlesinger; Bhaskar Bhatnagar; Donald Smith; Bruno Banceu; Ron Schoon; V.K. Sharma; Mark Kachmarsky; Srikant Ghantae; Michael Sorrels; Conal Deedy; Justin Clark; Skip Yeakel; Michael D. Laughlin; Charlotte Seigler; Sidney Diamond

    2007-04-30

    Class 8 heavy-duty trucks account for over three-quarters of the total diesel fuel used by commercial trucks (trucks with GVWRs more than 10,000 pounds) in the United States each year. At the highway speeds at which these trucks travel (i.e., 60 mph or greater), aerodynamic drag is a major part of total horsepower needed to move the truck down the highway, Reductions in aerodynamic drag can yield measurable benefits in fuel economy through the use of relatively inexpensive and simple devices. The goal of this project was to examine a number of aerodynamic drag reduction devices and systems and determine their effectiveness in reducing aerodynamic drag of Class 8 tractor/semitrailer combination-units, thus contributing to DOE's goal of reducing transportation petroleum use. The project team included major heavy truck manufacturers in the United States, along with the management and industry expertise of the Truck Manufacturers Association as the lead investigative organization. The Truck Manufacturers Association (TMA) is the national trade association representing the major North American manufacturers of Class 6-8 trucks (GVWRs over 19,500 lbs). Four major truck manufacturers participated in this project with TMA: Freightliner LLC; International Truck and Engine Corporation; Mack Trucks Inc.; and Volvo Trucks North America, Inc. Together, these manufacturers represent over three-quarters of total Class 8 truck sales in the United States. These four manufacturers pursued complementary research efforts as part of this project. The project work was separated into two phases conducted over a two-year period. In Phase I, candidate aerodynamic devices and systems were screened to focus research and development attention on devices that offered the most potential. This was accomplished using full-size vehicle tests, scale model tests, and computational fluid dynamics analyses. In Phase II, the most promising devices were installed on full-size trucks and their effect on fuel economy was determined, either through on-road testing or full-size wind tunnel testing. All of the manufacturers worked with devices and systems that offer practical solutions to reduce aerodynamic drag, accounting for functionality, durability, cost effectiveness, reliability, and maintainability. The project team members and their roles and responsibilities are shown in Figure 2-1. Figure 2-2 shows the Phase I and II project schedules for all four projects and associated management activities.

  2. Sampling Plan: Engineering Sampling Plan to Identify Areas for Remediation in the Southeast Drainage (Vicinity Properties DA-4 and DOC-7) Addendum 1. DOE/OR/21548-582

    Office of Legacy Management (LM)

  3. fn{EE49F893-CA64-40D2-9A32-E9DA8936271E}EIMS+Content&dbwisle@srn.sandia.gov.vsd

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Errors and Omissions Process ID: PCS.062 Revision #: 5 Revision Date: 04/27/2012 Page: 1 of 2 Task Owner: * PA Project Associate * DS Design Supplier * SCR Sandia Contracting Representative Process Owner: Senior Manager Customer Operations and Projects Printed Copies Of This Document are NOT Controlled Follow the Continual Improvement Process to Update this Process Manage Change FMS Design Error or Omission (DEO) PA Determines Associated Cost of NVA Error & Omission Cost Estimating NVA Cost

  4. Alternative Fuels Data Center: Maps and Data

    Alternative Fuels and Advanced Vehicles Data Center [Office of Energy Efficiency and Renewable Energy (EERE)]

    Generatedthumb20130810-31804-53z5da Carbon Intensity of Alternative Fuels in California Heavy-Duty Vehicles Generatedthumb20130810-31804-53z5da California Low Carbon Fuel ...

  5. Slide 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    DaBrisha Smith November 14, 2013 Page 2 ORP Safety Culture Update * How did the leadership transition go? - SC Team Lead turnover from Steve Pfaff to DaBrisha Smith has gone ...

  6. US ITER | Media Corner

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    India DA Project Director Visits US ITER Project Office India DA Project Director Visits US ITER Project Office Published Janaury 5, 2012 India DA Project Director Visits US ITER Project Office US ITER project manager Ned Sauthoff and India DA project director Shishir Deshpande share a laugh while watching a skit during the US ITER staff holiday luncheon. Photo: US ITER/ORNL Shishir P. Deshpande, Project Director for the ITER India Domestic Agency, visited the US ITER Project Office on December

  7. Final report: Efficient and user friendly C++ library for differential algebra

    SciTech Connect (OSTI)

    Svetlana G. Shasharina

    1998-09-29

    In Phase I we proposed the following tasks: Task 1: Identify the units of the Differential Algebra (DA) library, i.e. determine the abstract data types of the DA and the relations between them. Develop the interfaces (.h files) for the DA vectors. Task 2: Implement the DA vector class with garbage collection and expression templates for optimizing all overloaded operators by minimizing creation of temporaries and fusing loops. Task 3: Implement the prototype GUI for instantiating systems from files and invoking the DA methods. Task 4: Develop a suite of tests for the DA vector class and the needed utilities classes. Task 5: Write the final report on this work. This will include documentation on the use of the code. We have completed these tasks. In this section we discuss the results of our work.

  8. Nonplanar dust acoustic solitary waves in a strongly coupled dusty plasma with superthermal ions

    SciTech Connect (OSTI)

    El-Labany, S. K. Zedan, N. A.; El-Taibany, W. F. E-mail: eltaibany@du.edu.eg; El-Shamy, E. F.

    2014-12-15

    The nonplanar amplitude modulation of dust acoustic (DA) envelope solitary waves in a strongly coupled dusty plasma (SCDP) has been investigated. By using a reductive perturbation technique, a modified nonlinear Schrdinger equation (NLSE) including the effects of geometry, polarization, and ion superthermality is derived. The modulational instability (MI) of the nonlinear DA wave envelopes is investigated in both planar and nonplanar geometries. There are two stable regions for the DA wave propagation strongly affected by polarization and ion superthermality. Moreover, it is found that the nonlinear DA waves in spherical geometry are the more structurally stable. The larger growth rate of the nonlinear DA MI is observed in the cylindrical geometry. The salient characteristics of the MI in the nonplanar geometries cannot be found in the planar one. The DA wave propagation and the NLSE solutions are investigated both analytically and numerically.

  9. TECHNICAL/PEER REVIEW RECORD FORM PS-3 Pressure System Number

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    TECHNICAL/PEER REVIEW RECORD FORM PS-3 Pressure System Number Component(s) (if applicable) Design Authority (DA) DA Group/Division Note: Excluded Elements require a Peer Review. Peer Review must be completed by one or more DAs not associated with the project. Technical Review is applicable to code compliant components and can be performed by any DA. Type of Review (check) ____Technical Review ____Peer Review Description: Scope of Review: Applicable Code(s): The undersigned have reviewed the

  10. Building a Smarter Distribution System in Pennsylvania

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    PPL is installing a distribution management system (DMS), distribution automation (DA) ... allows PPL to move forward with future automation projects. "Lack of an advanced DMS was ...

  11. Destilaria Americana SA | Open Energy Information

    Open Energy Info (EERE)

    search Name: Destilaria Americana SA Place: Nova Amrica da Colina, Parana, Brazil Zip: 86230-000 Product: Ethanol producer in Paran, Brazil. References: Destilaria...

  12. Browse by Discipline -- E-print Network Subject Pathways: Renewable...

    Office of Scientific and Technical Information (OSTI)

    Detector Gro)up Buscaglia, Gustavo C. (Gustavo C. Buscaglia) - Departamento de Cincia da Computao, Universidade de So Paulo Go back to Individual Researchers ...

  13. A Measuring

    Office of Scientific and Technical Information (OSTI)

    ... SUMMARY Investigating DA release in response to drug adniinistration in vivo in the human ... process disclosed, or represents that its use would not infringe privately owned rights. ...

  14. ARM - Publications: Science Team Meeting Documents

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    D.A., Analytical Services and Materials, Inc.; Minnis, P., and Young, D.F., National Aeronautics and Space Administration-Langley Research Center Ninth Atmospheric Radiation...

  15. Record-Setting Microscopy Illuminates Energy Storage Materials

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    for Energy Research Applications (CAMERA). Handout Research conducted by: D.A. Shapiro (Advanced Light Source), Y.S. Yu (Lawrence Berkeley National Laboratory, University...

  16. Record-Setting Microscopy Illuminates Energy Storage Materials

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Division and runs optimized reconstruction algorithms developed by the Center for Applied Mathematics for Energy Research Applications (CAMERA). Handout Research conducted by: D.A....

  17. Pulsed Power Technology at Sandia National Laboratories

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Technology Programs and Capabilities Experimental and Theoretical Programs Electromagnetic Technology at Sandia National Laboratories HEDP & ICF Simulation Codes ALEGRA Spect3D--A...

  18. Chemical Physics | The Ames Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Key Scientific Personnel: Da-Jiang Liu, Michael Schmidt. The theoretical Chemical Physics ... of Basic Energy Sciences, Division of Chemical Sciences, Geosciences, and Biosciences ...

  19. Gary Trott

    Energy Savers [EERE]

    ENTROPY Lighting Controls are not Simple "Simplicity is the ultimate sophistication." - Leonardo Da Vinci Lighting Controls - Switched Installation Commissioning fdfgfdg Layout...

  20. Evolved strains of Scheffersomyces stipitis achieving high ethanol...

    Office of Scientific and Technical Information (OSTI)

    Authors: Slininger, Patricia J. 1 ; Shea-Andersh, Maureen A. 1 ; Thompson, Stephanie R. 1 ; Dien, Bruce S. 1 ; Kurtzman, Cletus P. 2 ; Balan, Venkatesh 3 ; da Costa ...

  1. Evolved strains of Scheffersomyces stipitis achieving high ethanol...

    Office of Scientific and Technical Information (OSTI)

    ... Authors: Slininger, Patricia J. 1 ; Shea-Andersh, Maureen A. 1 ; Thompson, Stephanie R. 1 ; Dien, Bruce S. 1 ; Kurtzman, Cletus P. 2 ; Balan, Venkatesh 3 ; da Costa ...

  2. CPL Participacoes Ltda | Open Energy Information

    Open Energy Info (EERE)

    Participacoes Ltda Jump to: navigation, search Name: CPL Participacoes Ltda Place: Salvador, Estado da Bahia, Brazil Sector: Wind energy Product: Wind farm developer and...

  3. Onsite Gaseous Centrifuge Enrichment Plant UF6 Cylinder Destructive Analysis

    SciTech Connect (OSTI)

    Anheier, Norman C.; Cannon, Bret D.; Qiao, Hong; Carter, Jennifer C.; McNamara, Bruce K.; O'Hara, Matthew J.; Phillips, Jon R.; Curtis, Michael M.

    2012-07-17

    The IAEA safeguards approach for gaseous centrifuge enrichment plants (GCEPs) includes measurements of gross, partial, and bias defects in a statistical sampling plan. These safeguard methods consist principally of mass and enrichment nondestructive assay (NDA) verification. Destructive assay (DA) samples are collected from a limited number of cylinders for high precision offsite mass spectrometer analysis. DA is typically used to quantify bias defects in the GCEP material balance. Under current safeguards measures, the operator collects a DA sample from a sample tap following homogenization. The sample is collected in a small UF6 sample bottle, then sealed and shipped under IAEA chain of custody to an offsite analytical laboratory. Current practice is expensive and resource intensive. We propose a new and novel approach for performing onsite gaseous UF6 DA analysis that provides rapid and accurate assessment of enrichment bias defects. DA samples are collected using a custom sampling device attached to a conventional sample tap. A few micrograms of gaseous UF6 is chemically adsorbed onto a sampling coupon in a matter of minutes. The collected DA sample is then analyzed onsite using Laser Ablation Absorption Ratio Spectrometry-Destructive Assay (LAARS-DA). DA results are determined in a matter of minutes at sufficient accuracy to support reliable bias defect conclusions, while greatly reducing DA sample volume, analysis time, and cost.

  4. Brasil Bio Fuels | Open Energy Information

    Open Energy Info (EERE)

    Bio Fuels Jump to: navigation, search Name: Brasil Bio Fuels Place: So Joo da Baliza, Roraima, Brazil Product: Brazil based ethanol producer located in Roraima, Brazil....

  5. Geochemical Behaviour of S, Cl and Fe in Silicate Melts/Glasses...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    E. Paris, P. Glatzel, S. Eeckhout, M. Carroll, School of Science and Technology, Geology Division, University of Camerino, Via G. III da Varano, 62032 Camerino; e-mail:...

  6. Gamesa Services Brasil Ltda | Open Energy Information

    Open Energy Info (EERE)

    Jump to: navigation, search Name: Gamesa Services Brasil Ltda Place: Simes Filho, Estado da Bahia, Brazil Sector: Wind energy Product: Wind farm developer and independent...

  7. Dedini A ucar e lcool | Open Energy Information

    Open Energy Info (EERE)

    to: navigation, search Name: Dedini Aucar e lcool Place: So Ja da Boa Vista, Sao Paulo, Brazil Zip: 13870 Product: Dedini Aucar e lcool is a local...

  8. Categorical ExclusionUetermlnatton Fonn

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and Guidelines DAI4. Approval oftechnieal exchange arrangements DA 15 - International umbrella agreements for energy R&D Facility Operations DB 1.2 - Training exercises and...

  9. Lisbon, Portugal: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    Energy Companies in Lisbon, Portugal EDP Renovaveis Martifer Renewables Formerly Eviva Mercado Abastecedor da Regiao de Lisboa MARL Solar Plus SA References http:...

  10. Augmenting system reliability analyses with observation priors...

    Office of Scientific and Technical Information (OSTI)

    missing data may be imputed using standard data augmentation (DA). This process is already used in the current implementation of the JMP complex-system reliability modeling codes. ...

  11. This form may be submitted to the EIA by mail, fax, e-mail, or...

    U.S. Energy Information Administration (EIA) Indexed Site

    ...www.eia.govsurveyformeia782alist782a.pdf" "Phone No.:",,,..."Ex... you are reporting:" "Type of Report (Check One):" ,,"Original",,,..."Mo",,,"Da...

  12. Search for: All records | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    ... Isolation of a significant fraction of non-phototroph diversity from a desert Biological Soil Crust Nunes da Rocha, Ulisses ; Cadillo-Quiroz, Hinsby ; Karaoz, Ulas ; Rajeev, Lara ; ...

  13. Search for: All records | DOE PAGES

    Office of Scientific and Technical Information (OSTI)

    Brodie, Eoin L. (1) Buenrostro, Mayra (1) Cadillo-Quiroz, Hinsby (1) Chanal, Angelique (1) ... Crust Nunes da Rocha, Ulisses ; Cadillo-Quiroz, Hinsby ; Karaoz, Ulas ; Rajeev, Lara ; ...

  14. A Case Study of Lean Implementation at Sandia National Laboratories...

    Office of Scientific and Technical Information (OSTI)

    Title: A Case Study of Lean Implementation at Sandia National Laboratories. Abstract not provided. Authors: de Luna, Raul ; Lopez, Mike R. ; Wan, Hung-da ; Chen, Frank F. ...

  15. Development of fine-resolution analyses and expanded large-scale...

    Office of Scientific and Technical Information (OSTI)

    The Community Gridpoint Statistical Interpolation system is implemented in a multiscale data assimilation (MS-DA) framework that is used within the Weather Research and Forecasting ...

  16. Parameterizing the Mixing State of Complex Submicron Aerosols...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    DA Knopf, MK Gilles, and RC Moffet. 2015. "Chemical imaging of ambient aerosol particles: Observational constraints on mixing state parameterization." Journal of Geophysical...

  17. Adsorptive Films in Support of In-field UF6 Destructive Assay Sample Collection and Analysis

    SciTech Connect (OSTI)

    Barrett, Christopher A.; Martinez, Alonzo; McNamara, Bruce K.; Cannon, Bret D.; Anheier, Norman C.

    2014-07-20

    International Atom Energy Agency (IAEA) safeguard verification measures in gaseous centrifuge enrichment plants (GCEPs) rely on environmental sampling, non-destructive assay (NDA), and destructive assay (DA) sampling and analysis to determine uranium enrichment. UF6 bias defect measurements are made by DA sampling and analysis to assure that enrichment is consistent with declarations. DA samples are collected from a limited number of cylinders for high precision, offsite mass spectrometer analysis. Samples are typically drawn from a sampling tap into a UF6 sample bottle, then packaged, sealed, and shipped under IAEA chain of custody to an offsite analytical laboratory. Future DA safeguard measures may require improvements in efficiency and effectiveness as GCEP capacities increase and UF6 shipping regulations become increasingly more restrictive. The Pacific Northwest National Laboratory (PNNL) DA sampler concept and Laser Ablation Absorption Ratio Spectrometry (LAARS) assay method are under development to potentially provide DA safeguard tools that increase inspection effectiveness and reduce sample shipping constraints. The PNNL DA sampler concept uses a handheld sampler to collect DA samples for either onsite LAARS assay or offsite laboratory analysis. The DA sampler design will use a small sampling planchet that is coated with an adsorptive film to collect controlled quantities of UF6 gas directly from a cylinder or process sampling tap. Development efforts are currently underway at PNNL to enhance LAARS assay performance to allow high-precision onsite bias defect measurements. In this paper, we report on the experimental investigation to develop adsorptive films for the PNNL DA sampler concept. These films are intended to efficiently capture UF6 and then stabilize the collected DA sample prior to onsite LAARS or offsite laboratory analysis. Several porous material composite films were investigated, including a film designed to maximize the chemical adsorption and binding of gaseous UF6 onto the sampling planchet.

  18. Timeline

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Timeline Timeline Date Event July 1, 2010 Charging starts. June 16, 2010 DaVinci decommisioned. Last DaVinci user logins on June 15. May 12, 2010 All active NERSC user accounts enabled. April 27, 2010 Selected NERSC user accounts enabled. January 26, 2010 System arrived. Last edited: 2016-04-29 11:35:04

  19. Microsoft Word - summer.doc

    U.S. Energy Information Administration (EIA) Indexed Site

    b trac ting fro m th e da ily a ve rag e hig h te m p e ra tu res fo r th e la st 10 y ea rs a n am o un t e qu al to tw ice a n estim ate o f the stan da rd de via tion for h igh...

  20. U.S. Geological Survey Open-File Report 99-310

    National Nuclear Security Administration (NNSA)

    r e d fr om Gr a v i t y D a ta , Ne va da Test Si te , N e va da by G.A. Phelps 1 , V.E. ... Figure 1. Map showing simplified geology of the Nevada Test Site region. White, Cenozoic ...

  1. A=19F (1983AJ01)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    1978DA1N, 1978MA2H, 1979DA15, 1980KU05, 1980MC1L, 1981ER03, 1981GR06, 1982KI02). Cluster, collective and rotational models: (1977BU22, 1977FO1E, 1978BR21, 1978CH26,...

  2. Head-on collision of dust-acoustic shock waves in strongly coupled dusty plasmas

    SciTech Connect (OSTI)

    EL-Shamy, E. F.; Al-Asbali, A. M.

    2014-09-15

    A theoretical investigation is carried out to study the propagation and the head-on collision of dust-acoustic (DA) shock waves in a strongly coupled dusty plasma consisting of negative dust fluid, Maxwellian distributed electrons and ions. Applying the extended PoincarLighthillKuo method, a couple of KortewegdeVriesBurgers equations for describing DA shock waves are derived. This study is a first attempt to deduce the analytical phase shifts of DA shock waves after collision. The impacts of physical parameters such as the kinematic viscosity, the unperturbed electron-to-dust density ratio, parameter determining the effect of polarization force, the ion-to-electron temperature ratio, and the effective dust temperature-to-ion temperature ratio on the structure and the collision of DA shock waves are examined. In addition, the results reveal the increase of the strength and the steepness of DA shock waves as the above mentioned parameters increase, which in turn leads to the increase of the phase shifts of DA shock waves after collision. The present model may be useful to describe the structure and the collision of DA shock waves in space and laboratory dusty plasmas.

  3. A novel PGC-1α isoform in brain localizes to mitochondria and associates with PINK1 and VDAC

    SciTech Connect (OSTI)

    Choi, Joungil; Veterans Affairs Medical Center, Baltimore, MD 21201 ; Batchu, Vera Venkatanaresh Kumar; Veterans Affairs Medical Center, Baltimore, MD 21201 ; Schubert, Manfred; Castellani, Rudolph J.; Russell, James W.; Veterans Affairs Medical Center, Baltimore, MD 21201

    2013-06-14

    Highlights: •Novel 35 kDa PGC-1α localizes to mitochondrial inner membrane and matrix in brain. •Mitochondrial localization of 35 kDa PGC-1α depends on VDAC protein. •Mitochondrial localization of 35 kDa PGC-1α depends on membrane potential. •The 35 kDa PGC-1α associates and colocalizes with PINK in brain mitochondria. -- Abstract: Peroxisome proliferator-activated receptor-gamma co-activator 1α (PGC-1α) and PTEN-induced putative kinase 1 (PINK1) are powerful regulators of mitochondrial function. Here, we report that a previously unrecognized, novel 35 kDa PGC-1α isoform localizes to the mitochondrial inner membrane and matrix in brain as determined by protease protection and carbonate extraction assays, as well as by immunoelectron microscopy. Immunoelectron microscopy and import experiments in vitro revealed that 35 kDa PGC-1α colocalizes and interacts with the voltage-dependent anion channel (VDAC), and that its import depends on VDAC. Valinomycin treatment which depolarizes the membrane potential, abolished mitochondrial localization of the 35 kDa PGC-1α. Using blue native-PAGE, co-immunoprecipitation, and immunoelectron microscopy analyses, we found that the 35 kDa PGC-1α binds and colocalizes with PINK1 in brain mitochondria. This is the first report regarding mitochondrial localization of a novel 35 kDa PGC-1α isoform and its association with PINK1, suggesting possible regulatory roles for mitochondrial function in the brain.

  4. Discriminant forest classification method and system

    DOE Patents [OSTI]

    Chen, Barry Y.; Hanley, William G.; Lemmond, Tracy D.; Hiller, Lawrence J.; Knapp, David A.; Mugge, Marshall J.

    2012-11-06

    A hybrid machine learning methodology and system for classification that combines classical random forest (RF) methodology with discriminant analysis (DA) techniques to provide enhanced classification capability. A DA technique which uses feature measurements of an object to predict its class membership, such as linear discriminant analysis (LDA) or Andersen-Bahadur linear discriminant technique (AB), is used to split the data at each node in each of its classification trees to train and grow the trees and the forest. When training is finished, a set of n DA-based decision trees of a discriminant forest is produced for use in predicting the classification of new samples of unknown class.

  5. Nova Alinca Agricola e Comercial | Open Energy Information

    Open Energy Info (EERE)

    Alinca Agricola e Comercial Jump to: navigation, search Name: Nova Alinca Agricola e Comercial Place: So Joaquim da Barra, Sao Paulo, Brazil Zip: 14.600-000 Product: Sao...

  6. Acoustic subwavelength imaging of subsurface objects with acoustic...

    Office of Scientific and Technical Information (OSTI)

    liuxiaojun@nju.edu.cn 1 ; State Key Laboratory of Acoustics, Institute of Acoustics, Chinese Academy of Sciences, Beijing 100190 2 ; Zhou, Chen ; Wei, Qi ; Wu, DaJian 1 +...

  7. BPA-2011-00504-FOIA Request

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Atterbu ry , aura M - -7 . CEIVED BY BPA Oi: OFFICE THIS From: rchapman , < < is I * 2 Sent: Thursday, Janua ry 20, 2011 4:42 PM ,;t E DA1E: Subject: FOIA Request Z-" I e The...

  8. Quickstart Resume Template

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... Biological Invasions Kierepka, E.M., S.D. Unger, D.A. Keiter, J.C. Beasley, O.E. Rhodes, ... University students at Crane Naval Warfare Center deer check station during deer ...

  9. Universal Charge Order in the High-Tc Superconductors | Stanford...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Eduardo H. da Silva Neto was born in Recife, Brazil. He obtained his B.A. in Physics and Mathematics (2008) from Amherst College, and his Ph. D. (2013) in Physics from Princeton ...

  10. Knoxville Utilities Board Smart Grid Project | Open Energy Information

    Open Energy Info (EERE)

    Thermostats Customer Systems for 4,200 customers Home Area Networks Web Portal Access In-Home DisplaysEnergy Management Systems Distribution Automation (DA) Equipment for 5 out...

  11. Progress Toward Attractive Stellarators

    Office of Scientific and Technical Information (OSTI)

    9 PPPL- 4589 Progress Toward Attractive Stellarators January, 2011 G.H. Neilson, L. Bromberg, T.G. Brown, D.A. Gates, L.P. Ku, M.C. Zarnstorff, A.H. Boozer, J.H. Harris, O. ...

  12. BPA-2015-01323-FOIA Response

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ave., N.W., Suite 720 Washington, DC 20006 Main: 202.787.1900 Shelly DaMore Jordan Ramis PC P.O. Box 230669 Portland, OR 97281 E-mail: shelly.damore@jordanramis.com...

  13. A Key Enzyme to the Potency of an Anticancer Agent

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    that 5'-ClDA is a biosynthesis intermediate. SalL recombinant protein purified from E. coli organizes as a homotrimer (30 kDamonomer). Biochemical in vitro analyses showed it...

  14. X-ray Diffraction from Membrane Protein Nanocrystals

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    X-ray Diffraction from Membrane Protein Nanocrystals Authors: Hunter, M.S., DePonte, D.P., Shapiro, D.A., Kirian, R.A., Wang, X., Starodub, D., Marchesini, S., Weierstall, U.,...

  15. Search for: All records | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    Bearinger, Jane P. (1) Da Silva, Luiz (1) Jensen, Wayne A. (1) Loge, Jeffrey M. (1) ... IV, Ward ; Schumann, Daniel L. ; Jensen, Wayne A. ; Ortega, Jason M. ; Marion, III, John ...

  16. ARM - Selected Science Team Proposals - FY 2000

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Data" NEW- Drs. Edward V. BrowellRichard A. Ferrare, NASA Langley Research ... David L Mitchell W. Patrick ArnottDa Koracin, Desert Research Institute: "A New Treatment of ...

  17. March Events

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    event highlights Mar 2 Wed 8:00 AM CoDA 2016, the Conference on Data Analysis Eldorado Hotel - 309 W. San Francisco St., Santa Fe, NM Join us for the Conference on Data Analysis,...

  18. Title

    National Nuclear Security Administration (NNSA)

    (USGS Open file Report 92-xxxx) Author Laczniak, R. J., Cole, J. C, Sawyer D.A. and Trudeau, D. A. Document Date 5792 Document Type Report Recipients DOENV 100960 ...

  19. 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... March 14-18, 2005 Mlawer, EJ, TR Shippert, CN Long, MA Miller, KL Johnson, DT Troyan, GG Mace, SA Clough, MH Zhang, SC Xie, RT Cederwall, JJ Yio, DR Doelling, DA Rutan, DD ...

  20. A New Light on Disordered Ensembles

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    M.J. Bogan (SLAC National Accelerator Laboratory); S. Marchesini (ALS); D.A. Shapiro (Brookhaven National Laboratory); and H.C. Poon and D.K. Saldin (University of...

  1. Iodine valence and local environments in borosilicate waste glasses...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    319 5226; fax: +1 (202) 319 4469. E-mail address: davidm@vsl.cua.edu (D.A. McKeown). Journal of Nuclear Materials 456 (2015) 182-191 Contents lists available at ScienceDirect...

  2. untitled

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    te nos anos de 2009 e 2010 na ilha Gracio- sa vai voltar, desta feita em definitivo, em ... 2009 e 2010, quais foram os dados mais impor- tantes apurados pelaestao daGracio- sa? ...

  3. Competing charge, spin, and superconducting orders in underdoped...

    Office of Scientific and Technical Information (OSTI)

    Ruixing ; Bonn, D.A. ; Hardy, W.N. ; Gutowski, O. ; Zimmermann, M.v. ; Hayden, S.M. ; Chang, J. 1 ; Denmark) 2 ; UBC) 2 ; DESY) 2 ; Ecole) 2 ; CIFAR) 2 ; Bristol) 2 ...

  4. A Key Enzyme to the Potency of an Anticancer Agent

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    SalL, however, uses chloride to displace L-methionine from S-adenosyl-L-methionine (SAM) and generate 5'-chloro-5'deoxyadenosine (5'-ClDA) in a rarely observed nucleophilic...

  5. Hybrid Rotaxanes: Interlocked Structures for Quantum Computing...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    modifications of the chemistry. Research conducted by C.-F. Lee, D.A. Leigh, and D. Schultz, (University of Edinburgh, UK); R.G. Pritchard, G.A. Timco, and R.E.P. Winpenny...

  6. Browse by Discipline -- E-print Network Subject Pathways: Environmenta...

    Office of Scientific and Technical Information (OSTI)

    S T U V W X Y Z Saha, Tapan Kumar (Tapan Kumar Saha) - School of Information Technology and Electrical Engineering, University of Queensland Silva, Filipe Faria Da (Filipe Faria ...

  7. The Hydrogen Laboratory and The Brazilian Reference Center for...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... impacts, possible hydrogen sources, energy needs and mix Thank you Contacts: Newton Pimenta Neves Jr. nevesjr@ifi.unicamp.br Cristiano da Silva Pinto cspinto@ifi.unicamp.br

  8. Cinco de Mayo

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    May 5. It is celebrated in the United States and in Mexico, primarily in the state of Puebla, where the holiday is called El Da de la Batalla de Puebla (English: The Day of the...

  9. Search for: All records | DOE PAGES

    Office of Scientific and Technical Information (OSTI)

    Dien, Bruce S. (1) Kurtzman, Cletus P. (1) Shea-Andersh, Maureen A. (1) Slininger, Patricia J. (1) Thompson, Stephanie R. (1) Uppugundla, Nirmal (1) da Costa Sousa, Leonardo (1) ...

  10. Search for: All records | DOE PAGES

    Office of Scientific and Technical Information (OSTI)

    Dien, Bruce S. (1) Kurtzman, Cletus P. (1) Shea-Andersh, Maureen A. (1) Slininger, Patricia J. (1) Thompson, Stephanie R. (1) Xue, Saisi (1) da Costa Sousa, Leonardo (1) Save ...

  11. ARM - Publications: Science Team Meeting Documents

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Clear-Sky Model and Measurement Comparisons from the First Diffuse Irradiance IOP - Fall 2001 Powell, D.(a), Kato, S.(b), Haeffelin, M.(c), and Dubovik, O.(d), Pacific Northwest...

  12. Material Protection, Accounting and Control

    Energy Savers [EERE]

    ... accuracy and precision of Pu analysis by gamma spectroscopy n Close gap between NDA and DA methods (<0.1% vs 1%) n Reduce reliance on sampling and mass spectrometry ...

  13. AMENDMENT OF SOLICITATION/MODIFICATION OF CONTRACT 11. CONTRACT...

    National Nuclear Security Administration (NNSA)

    l PAGE OF PAGES 1 I 3 2. AMENDMENTMODIFICATION NO. See Block 16C 4. REQUISITIONPURCHASE REQ. NO. 15. PROJECT NO. (If applicable) 3. EFFECTIVE DA TE 0264 6. ISSUED BY CODE 05003 ...

  14. ARM - Publications: Science Team Meeting Documents

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Impact of Clouds on the Atmospheric Absorption of SW - Comparing Theory and Observation at SGP Rose, F.G. (a), Charlock, T.P. (b), and Rutan, D.A. (a), Analytical Services &...

  15. Wave Energy Centre | Open Energy Information

    Open Energy Info (EERE)

    Centre Jump to: navigation, search Name: Wave Energy Centre Address: Wave Energy Centre Av Manuela da Maia 36 R C Dto Place: Lisboa Zip: 1000-201 Region: Portugal Sector: Marine...

  16. Search for: All records | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    ... ; Febres Cordero, F. ; Forde, D. ; Ita, H. ; Kosower, D.A. ; Maitre, D. ; MIT, LNS Santa Barbara, KITP SLAC UCLA Saclay Full Text Available April 2008 Mesurement of the ...

  17. Ocean Thermal Energy Conversion (OTEC) | Seawater Cooling - Depth...

    Open Energy Info (EERE)

    Author National Renewable Energy Laboratory Maintainer Nicholas Langle bureaucode 019:20 Catalog DOE harvestobjectid 3ba3acfd-d54a-4a3d-a971-1cf4ac97fcb0 harvestsourceid...

  18. BPA-2011-01782-FOIA Request

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    F 1 ET 1S The following is a New FOIA request: DATE: Q DUE DA E: Name: Richard van Dijk 4,ag-ZY .. F Organization: Another Way BPA Address: Phone: L OG J7 d 7 No FAX...

  19. Limite Marinha A Estrutura de Pesquisa Climtica do Programa...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    de cerca de 1 a 2 km. Num ambiente marinho, as nuvens da camada limite filtram o sol passivamente mas tambm funcionam como sistemas inter- activos que influenciam e...

  20. A=18Ne (1983AJ01)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    83AJ01) (See Energy Level Diagrams for 18Ne) GENERAL: See also (1978AJ03) and Table 18.21 Table of Energy Levels (in PDF or PS). Model calculations: (1979DA15, 1979SA31,...

  1. NREL's 91-Year-Old Palmer Carlin-a Wind Energy Pioneer | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    who was convinced he had a major discovery. After discussing the details of the invention with the modern-day Da Vinci, Carlin paused, and then asked the man if the invention...

  2. Smith Electric Vehicles SEV Group Ltd | Open Energy Information

    Open Energy Info (EERE)

    SEV Group Ltd Jump to: navigation, search Name: Smith Electric Vehicles (SEV) Group Ltd Place: Tyne & Wear, United Kingdom Zip: NE38 9DA Sector: Vehicles Product: UK-based...

  3. The European Physical Journal C CrossMark

    Office of Scientific and Technical Information (OSTI)

    de Janeiro, Rio de Janeiro, Brazil W. Carvalho, J. Chinellato6, A. Custodio, E. M. Da Costa, D. De Jesus Damiao, C. De Oliveira Martins, S. Fonseca De Souza, H. Malbouisson, D....

  4. Structural Studies of the Didomain of a 6-Deoxyerythronolide...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    supported by NIH grants CA 66736 and GM 22172 Primary Citation Tang, Y., Kim, C.-Y., Mathews, I. I., Cane, D. E., Khosla, C. (2006) The 2.7 Structure of a 194-kDa Homodimeric...

  5. Blog | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    A Centuries-old Tradition ItWe often associate renewable energy as future, forward-thinking technology. However, just as in the case of da Vinci's curved mirror - many of...

  6. DNI-predictability_paper

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    (e.g. due to wind stow) and periods of snow coverage of the pyrheliometer were removed. Mean DNI values for five minute intervals (for HA evaluation) and hourly intervals (for DA...

  7. BPA-2013-01353-FOIA Request

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    the time the request is submitted. Enter description: Zit I1 LI) K IWA OI '. oETICE TIUS DA DUE DATE: - LOG 7232013 Ex 6 A compete record of all competitive hiring events...

  8. Microsoft Word - Sorek_BioEngBugs_revised.docx

    Office of Scientific and Technical Information (OSTI)

    PanDaTox: a tool for accelerated metabolic engineering Gil Amitai1 and Rotem Sorek1'* 1Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel * Email for correspondence: rotem.sorek@weizmann.ac.il Abstract Metabolic engineering is often facilitated by cloning of genes encoding enzymes from various heterologous organisms into E. coli. Such engineering efforts are frequently hampered by foreign genes that are toxic to the E. coli host. We have developed PanDaTox

  9. Alkaline tolerant dextranase from streptomyces anulatus

    DOE Patents [OSTI]

    Decker, Stephen R. (Berthoud, CO); Adney, William S. (Golden, CO); Vinzant, Todd B. (Golden, CO); Himmel, Michael E. (Littleton, CO)

    2003-01-01

    A process for production of an alkaline tolerant dextranase enzyme comprises culturing a dextran-producing microorganism Streptomyces anulatus having accession no. ATCC PTA-3866 to produce an alkaline tolerant dextranase, Dex 1 wherein the protein in said enzyme is characterized by a MW of 63.3 kDa and Dex 2 wherein its protein is characterized by a MW of 81.8 kDa.

  10. Slide 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    HAB Briefing on ORP Organizational and Safety Culture Improvement Council DaBrisha Smith November 14, 2013 Page 2 ORP Safety Culture Update * How did the leadership transition go? - SC Team Lead turnover from Steve Pfaff to DaBrisha Smith has gone remarkably well. * Has anything changed on the team? - New Members * over half of the original 2012 team members changed out. We have received 8 new members: Nuclear Safety (1), Contracts (1), WTP Start & Commissioning (2), WED (1), TF (1),

  11. Addiction: Beyond dopamine reward circuitry

    SciTech Connect (OSTI)

    Volkow, N.D.; Wang, G.; Volkow, N.D.; Wang, G.-J.; Fowler, J.S.; Tomasi, D.; Telang, F.

    2011-09-13

    Dopamine (DA) is considered crucial for the rewarding effects of drugs of abuse, but its role in addiction is much less clear. This review focuses on studies that used PET to characterize the brain DA system in addicted subjects. These studies have corroborated in humans the relevance of drug-induced fast DA increases in striatum [including nucleus accumbens (NAc)] in their rewarding effects but have unexpectedly shown that in addicted subjects, drug-induced DA increases (as well as their subjective reinforcing effects) are markedly blunted compared with controls. In contrast, addicted subjects show significant DA increases in striatum in response to drug-conditioned cues that are associated with self-reports of drug craving and appear to be of a greater magnitude than the DA responses to the drug. We postulate that the discrepancy between the expectation for the drug effects (conditioned responses) and the blunted pharmacological effects maintains drug taking in an attempt to achieve the expected reward. Also, whether tested during early or protracted withdrawal, addicted subjects show lower levels of D2 receptors in striatum (including NAc), which are associated with decreases in baseline activity in frontal brain regions implicated in salience attribution (orbitofrontal cortex) and inhibitory control (anterior cingulate gyrus), whose disruption results in compulsivity and impulsivity. These results point to an imbalance between dopaminergic circuits that underlie reward and conditioning and those that underlie executive function (emotional control and decision making), which we postulate contributes to the compulsive drug use and loss of control in addiction.

  12. Development of Patients' Decision Aid for Older Women With Stage I Breast Cancer Considering Radiotherapy After Lumpectomy

    SciTech Connect (OSTI)

    Wong, Jennifer; D'Alimonte, Laura; Angus, Jan; Paszat, Larry; Metcalfe, Kelly; Whelan, Tim; Llewellyn-Thomas, Hilary; Warner, Eiran; Franssen, Edmee; Szumacher, Ewa

    2012-09-01

    Purpose: To develop a patient decision aid (PtDA) for older women with Stage I, pathologically node negative, estrogen receptor-positive progesterone receptor-positive breast cancer who are considering adjuvant radiotherapy after lumpectomy and to examine its impact on patients' decision making. Methods and Materials: A PtDA was developed and evaluated in three steps according to the Ottawa Decision Support Framework: (1) needs assessment (n = 16); (2) Pilot I to examine PtDA acceptability (n = 12); and (3) Pilot II, a pretest posttest (n = 38) with older women with estrogen receptor-positive progesterone receptor-positive breast cancer after lumpectomy who were receiving adjuvant radiation therapy. Measures included patients' satisfaction with the PtDA, self-reported decisional conflict, level of distress, treatment-related knowledge, and choice predisposition. Results: The PtDA is a booklet that details each adjuvant treatment option's benefits, risks, and side effects tailored to the patient's clinical profile; includes a values clarification exercise; and includes steps to guide patients towards their decision. On the basis of qualitative comments and satisfaction ratings, all women thought that the PtDA was helpful and informative. In comparison with their baseline scores, patients had a statistically significant (p < 0.05) reduction in decisional conflict (adjusted mean difference [AMD], -7.18; 95% confidence interval [CI], -13.50 to 12.59); increased clarity of the benefits and risks (AMD, -10.86; CI, -20.33 to 21.49); and improved general treatment knowledge (AMD, 8.99; CI, 2.88-10.28) after using the PtDA. General trends were also reported in the patients' choice predisposition scores that suggested potential differences in treatment decision after PtDA use. Conclusions: This study provides evidence that this PtDA may be a helpful educational tool for this group of women. The quality of care for older breast cancer patients may be enhanced by the use of a tailored PtDA to help patients be better informed about their treatment options.

  13. Comparative Study of Corn Stover Pretreated by Dilute Acid and Cellulose Solvent-Based Lignocellulose Fractionation: Enzymatic Hydrolysis, Supramolecular Structure, and Substrate Accessibility

    SciTech Connect (OSTI)

    Zhu, Z.; Sathitsuksanoh, N.; Vinzant, T.; Schell, D. J.; McMillian, J. D.; Zhang, Y. H. P.

    2009-07-01

    Liberation of fermentable sugars from recalcitrant biomass is among the most costly steps for emerging cellulosic ethanol production. Here we compared two pretreatment methods (dilute acid, DA, and cellulose solvent and organic solvent lignocellulose fractionation, COSLIF) for corn stover. At a high cellulase loading [15 filter paper units (FPUs) or 12.3 mg cellulase per gram of glucan], glucan digestibilities of the corn stover pretreated by DA and COSLIF were 84% at hour 72 and 97% at hour 24, respectively. At a low cellulase loading (5 FPUs per gram of glucan), digestibility remained as high as 93% at hour 24 for the COSLIF-pretreated corn stover but reached only {approx}60% for the DA-pretreated biomass. Quantitative determinations of total substrate accessibility to cellulase (TSAC), cellulose accessibility to cellulase (CAC), and non-cellulose accessibility to cellulase (NCAC) based on adsorption of a non-hydrolytic recombinant protein TGC were measured for the first time. The COSLIF-pretreated corn stover had a CAC of 11.57 m{sup 2}/g, nearly twice that of the DA-pretreated biomass (5.89 m{sup 2}/g). These results, along with scanning electron microscopy images showing dramatic structural differences between the DA- and COSLIF-pretreated samples, suggest that COSLIF treatment disrupts microfibrillar structures within biomass while DA treatment mainly removes hemicellulose. Under the tested conditions COSLIF treatment breaks down lignocellulose structure more extensively than DA treatment, producing a more enzymatically reactive material with a higher CAC accompanied by faster hydrolysis rates and higher enzymatic digestibility.

  14. 43-kilodalton protein of Torpedo nicotinic postsynaptic membranes: purification and determination of primary structure

    SciTech Connect (OSTI)

    Carr, C.; McCourt, D.; Cohen, J.B.

    1987-11-03

    The primary structure of the 43-kilodalton peripheral membrane protein (43-kDa protein) of Torpedo nicotinic postsynaptic membrane has been determined. The /sup 14/C-labelled 43-kDa protein, which was isolated by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis, has an amino terminus resistant to Edman degradation, while the sequence at the carboxyl terminus is Tyr-Val. An amino acid sequence of 405 residues was obtained by NH/sub 2/-terminal sequence analysis of complementary peptides generated by digestion with trypsin, chymotrypsin, Staphylococcus aureus V8 protease, and endoproteinase Lys-C, as well as by chemical cleavage at methionine. This sequence of molecular mass 45,618 daltons lacks the amino terminus but extends to the carboxyl terminus of the 43-kDa protein. Unusual structural features of the 43-kDa protein include two regions of approx. 80 residues, each containing 10% cysteine, as well as stretches predicted to exist as amphipathic ..cap alpha..-helices. Other than the group blocking the amino terminus, no evidence was found for posttranslational modification of amino acids. The 43-kDa protein may represent a novel protein family because a computer search of this sequence with the National Biomedical Research Foundation data base (Release 12.0) did not reveal any significant homology to known protein sequences.

  15. Measurement of Fatigue Crack Growth Relationships in Hydrogen Gas for Pressure Swing Adsorber Vessel Steels

    SciTech Connect (OSTI)

    Somerday, Brian P.; Barney, Monica

    2014-12-04

    We measured the hydrogen-assisted fatigue crack growth rates (da/dN) for SA516 Grade 70 steel as a function of stress-intensity factor range (ΔK) and load-cycle frequency to provide life-prediction data relevant to pressure swing adsorber (PSA) vessels. For ΔK values up to 18.5 MPa m1/2, the baseline da/dN versus ΔK relationship measured at 1Hz in 2.8 MPa hydrogen gas represents an upper bound with respect to crack growth rates measured at lower frequency. However, at higher ΔK values, we found that the baseline da/dN data had to be corrected to account for modestly higher crack growth rates at the lower frequencies relevant to PSA vessel operation.

  16. Electrochemically-Modulated Separations for Material Accountability Measurements

    SciTech Connect (OSTI)

    Arrigo, Leah M.; Liezers, Martin; Douglas, Matthew; Green, Michael A.; Farmer, Orville T.; Schwantes, Jon M.; Peper, Shane M.; Duckworth, Douglas C.

    2010-05-07

    The Safeguards community recognizes that an accurate and timely measurement of accountable material mass at the head-end of the facility is critical to a modern materials control and accountability program at fuel reprocessing plants. For material accountancy, it is critical to detect both acute and chronic diversions of nuclear materials. Therefore, both on-line nondestructive (NDA) and destructive analysis (DA) approaches are desirable. Current methods for DA involve grab sampling and laboratory based column extractions that are costly, hazardous, and time consuming. Direct on-line gamma measurements of Pu, while desirable, are not possible due to contributions from other actinide and fission products. A technology for simple, online separation of targeted materials would benefit both DA and NDA measurements.

  17. Analytic Evolution of Singular Distribution Amplitudes in QCD

    SciTech Connect (OSTI)

    Radyushkin, Anatoly V.; Tandogan Kunkel, Asli

    2014-03-01

    We describe a method of analytic evolution of distribution amplitudes (DA) that have singularities, such as non-zero values at the end-points of the support region, jumps at some points inside the support region and cusps. We illustrate the method by applying it to the evolution of a flat (constant) DA, anti-symmetric at DA and then use it for evolution of the two-photon generalized distribution amplitude. Our approach has advantages over the standard method of expansion in Gegenbauer polynomials, which requires infinite number of terms in order to accurately reproduce functions in the vicinity of singular points, and over a straightforward iteration of an initial distribution with evolution kernel. The latter produces logarithmically divergent terms at each iteration, while in our method the logarithmic singularities are summed from the start, which immediately produces a continuous curve, with only one or two iterations needed afterwards in order to get rather precise results.

  18. Measurement of Fatigue Crack Growth Relationships in Hydrogen Gas for Pressure Swing Adsorber Vessel Steels

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Somerday, Brian P.; Barney, Monica

    2014-12-04

    We measured the hydrogen-assisted fatigue crack growth rates (da/dN) for SA516 Grade 70 steel as a function of stress-intensity factor range (ΔK) and load-cycle frequency to provide life-prediction data relevant to pressure swing adsorber (PSA) vessels. For ΔK values up to 18.5 MPa m1/2, the baseline da/dN versus ΔK relationship measured at 1Hz in 2.8 MPa hydrogen gas represents an upper bound with respect to crack growth rates measured at lower frequency. However, at higher ΔK values, we found that the baseline da/dN data had to be corrected to account for modestly higher crack growth rates at the lower frequenciesmore » relevant to PSA vessel operation.« less

  19. Head-on-collision of modulated dust acoustic waves in strongly coupled dusty plasma

    SciTech Connect (OSTI)

    El-Labany, S. K.; El-Depsy, A.; Zedan, N. A.; El-Taibany, W. F.; El-Shamy, E. F.

    2012-10-15

    The derivative expansion perturbation method is applied to a strongly coupled dusty plasma system consisting of negatively charged dust grains, electrons, and ions. The basic equations are reduced to a nonlinear Schroedinger type equation appropriate for describing the modulated dust acoustic (DA) waves. We have examined the modulation (in) stability and the dependence of the system physical parameters (angular frequency and group velocity) on the polarization force variation. Finally, the extended Poincare-Lighthill-Kuo technique is employed to investigate the head-on collision (HoC) between two DA dark solitons. The analytical phase shifts and the trajectories of these dark solitons after the collision are derived. The numerical illustrations show that the polarization effect has strong influence on the nature of the phase shifts and the trajectories of the two DA dark solitons after collision.

  20. Protein kinase C and its substrates in tumor promoter-sensitive and -resistant cells

    SciTech Connect (OSTI)

    Smith, B.M.; Colburn, N.H.

    1988-05-05

    Calcium- and phospholipid-dependent protein kinase C activity and substrates were characterized in cell lysates of preneoplastic JB6 cells, a model system of genetic variants for sensitivity to tumor promoter-induced neoplastic transformation. Protein kinase C activity was similar for sensitive and resistant variants, as measured by calcium- and phospholipid-dependent phosphorylation of an exogenous substrate (histone HIII). Of 13 endogenous protein kinase C substrates, identified by labeling proteins with (..gamma..-/sup 32/P) ATP, at least two (80 and 23 kDa) are potential candidates for mediating events on the pathway for promotion of transformation. /sup 32/P incorporation into the 80-kDa protein kinase C substrate was stimulated by tetradecanoylphorbol acetate and correlated with phenotype: the highest incorporation was found in promotion-insensitive cells, an intermediate level in promotion-sensitive cells and the lowest in the transformed cells. The phosphorylation of an 80-kDa protein, found by labeling intact cells in monolayer growth with (/sup 32/P)orthophosphate, was also stimulated by tetradecanoylphorbol acetate and correlated inversely with phenotype. The 80 kDa protein kinase C substrate from cells lysates and the 80-kDa phosphoprotein from intact cells appear to be identical, as indicated by peptide mapping with protease V8 from Staphylococcus aureus. This finding suggests that the 80-kDa substrate is relevant to promoter-induced signal transduction in the intact cell. In summary, there are no unique substrates that distinguish the variants. Quantitative differences in certain substrates or their phosphorylation may, however, account for the difference in promotion sensitivity among the variants.

  1. A Laser-Based Method for On-Site Analysis of UF6 at Enrichment Plants

    SciTech Connect (OSTI)

    Anheier, Norman C.; Cannon, Bret D.; Martinez, Alonzo; Barrett, Christopher A.; Taubman, Matthew S.; Anderson, Kevin K.; Smith, Leon E.

    2014-11-23

    The International Atomic Energy Agency’s (IAEA’s) long-term research and development plan calls for more cost-effective and efficient safeguard methods to detect and deter misuse of gaseous centrifuge enrichment plants (GCEPs). The IAEA’s current safeguards approaches at GCEPs are based on a combination of routine and random inspections that include environmental sampling and destructive assay (DA) sample collection from UF6 in-process material and selected cylinders. Samples are then shipped offsite for subsequent laboratory analysis. In this paper, a new DA sample collection and onsite analysis approach that could help to meet challenges in transportation and chain of custody for UF6 DA samples is introduced. This approach uses a handheld sampler concept and a Laser Ablation, Laser Absorbance Spectrometry (LAARS) analysis instrument, both currently under development at the Pacific Northwest National Laboratory. A LAARS analysis instrument could be temporarily or permanently deployed in the IAEA control room of the facility, in the IAEA data acquisition cabinet, for example. The handheld PNNL DA sampler design collects and stabilizes a much smaller DA sample mass compared to current sampling methods. The significantly lower uranium mass reduces the sample radioactivity and the stabilization approach diminishes the risk of uranium and hydrogen fluoride release. These attributes enable safe sample handling needed during onsite LAARS assay and may help ease shipping challenges for samples to be processed at the IAEA’s offsite laboratory. The LAARS and DA sampler implementation concepts will be described and preliminary technical viability results presented.

  2. SU-E-J-70: Feasibility Study of Dynamic Arc and IMRT Treatment Plans Utilizing Vero Treatment Unit and IPlan Planning Computer for SRS/FSRT Brain Cancer Patients

    SciTech Connect (OSTI)

    Huh, S; Lee, S; Dagan, R; Malyapa, R; Mendenhall, N; Mendenhall, W; Ho, M; Hough, D; Yam, M; Li, Z

    2014-06-01

    Purpose: To investigate the feasibility of utilizing Dynamic Arc (DA) and IMRT with 5mm MLC leaf of VERO treatment unit for SRS/FSRT brain cancer patients with non-invasive stereotactic treatments. The DA and IMRT plans using the VERO unit (BrainLab Inc, USA) are compared with cone-based planning and proton plans to evaluate their dosimetric advantages. Methods: The Vero treatment has unique features like no rotational or translational movements of the table during treatments, Dynamic Arc/IMRT, tracking of IR markers, limitation of Ring rotation. Accuracies of the image fusions using CBCT, orthogonal x-rays, and CT are evaluated less than ∼ 0.7mm with a custom-made target phantom with 18 hidden targets. 1mm margin is given to GTV to determine PTV for planning constraints considering all the uncertainties of planning computer and mechanical uncertainties of the treatment unit. Also, double-scattering proton plans with 6F to 9F beams and typical clinical parameters, multiple isocenter plans with 6 to 21 isocenters, and DA/IMRT plans are evaluated to investigate the dosimetric advantages of the DA/IMRT for complex shape of targets. Results: 3 Groups of the patients are divided: (1) Group A (complex target shape), CI's are same for IMRT, and DGI of the proton plan are better by 9.5% than that of the IMRT, (2) Group B, CI of the DA plans (1.91+/−0.4) are better than cone-based plan, while DGI of the DA plan is 4.60+/−1.1 is better than cone-based plan (5.32+/−1.4), (3) Group C (small spherical targets), CI of the DA and cone-based plans are almost the same. Conclusion: For small spherical targets, cone-based plans are superior to other 2 plans: DS proton and DA plans. For complex or irregular plans, dynamic and IMRT plans are comparable to cone-based and proton plans for complex targets.

  3. Defining the needs for non-destructive assay of UF6 feed, product, and tails at gas centrifuge enrichment plants and possible next steps

    SciTech Connect (OSTI)

    Boyer, Brian D; Swinhoe, Martyn T; Moran, Bruce W; Lebrun, Alain

    2009-01-01

    Current safeguards approaches used by the IAEA at gas centrifuge enrichment plants (GCEPs) need enhancement in order to detect undeclared LEU production with adequate detection probability using non destructive assay (NDA) techniques. At present inspectors use attended systems, systems needing the presence of an inspector for operation, during inspections to verify the mass and {sup 235}U enrichment of UF{sub 6} bulk material used in the process of enrichment at GCEPS. The inspectors also take destructive assay (DA) samples for analysis off-site which provide accurate, on the order of 0.1 % to 0.5% uncertainty, data on the enrichment of the UF{sub 6} feed, tails, and product. However, DA sample taking is a much more labor intensive and resource intensive exercise for the operator and inspector. Furthermore, the operator must ship the samples off-site to the IAEA laboratory which delays the timeliness of the results and contains the possibility of the loss of the continuity of knowledge of the samples during the storage and transit of the material. Use of the IAEA's inspection sampling algorithm shows that while total sample size is fixed by the total population of potential samples and its intrinsic qualities, the split of the samples into NDA or DA samples is determined by the uncertainties in the NDA measurements. Therefore, the larger the uncertainties in the NDA methods, more of the sample taken must be DA samples. Since the DA sampling is arduous and costly, improvements in NDA methods would reduce the number of DA samples needed. Furthermore, if methods of on-site analysis of the samples could be developed that have uncertainties in the 1-2% range, a lot of the problems inherent in DA sampling could be removed. The use of an unattended system that could give an overview of the entire process giving complementary data on the enrichment process as well as accurate measures of enrichment and weights of the UF{sub 6} feed, tails, and product would be a major step in enhancing the ability of NDA beyond present attended systems. The possibility of monitoring the feed, tails, and product header pipes in such a way as to gain safeguards relevant flow and enrichment information without compromising the intellectual property of the operator including proprietary equipment and operational parameters would be a huge step forward. This paper contains an analysis of possible improvements in unattended and attended NDA systems including such process monitoring and possible on-site analysis of DA samples that could reduce the uncertainty of the inspector measurements reducing the difference between the operator's and inspector's measurements providing more effective and efficient IAEA GeEPs safeguards.

  4. COLUMBIA RADIATION LABORATORY RESEARCH INVESTIGATION DIRECTED TOWARD EXTENDING

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    iVP-^"^^? COLUMBIA RADIATION LABORATORY RESEARCH INVESTIGATION DIRECTED TOWARD EXTENDING THE USEFUL RANGE OF THE ELECTROMAGNETIC SPECTRUM Special Technical Report Signal Corps Contract DA-36-039 SC-64630 DA Project No. 3-99-10-022 SC Project No. 102B U. S. Army Laboratory Procurement Office Signal Corps Supply Agency Fort Monmouth, New Jersey The Trustees of Columbia University in the City of New York Box 6, Low Memorial Library New York 27, New York March 1, 1956 DISCLAIMER Portions of

  5. TRACKING CODE DEVELOPMENT FOR BEAM DYNAMICS OPTIMIZATION

    SciTech Connect (OSTI)

    Yang, L.

    2011-03-28

    Dynamic aperture (DA) optimization with direct particle tracking is a straight forward approach when the computing power is permitted. It can have various realistic errors included and is more close than theoretical estimations. In this approach, a fast and parallel tracking code could be very helpful. In this presentation, we describe an implementation of storage ring particle tracking code TESLA for beam dynamics optimization. It supports MPI based parallel computing and is robust as DA calculation engine. This code has been used in the NSLS-II dynamics optimizations and obtained promising performance.

  6. The Complexes of Bisphosphonate and Magnetite Nanoparticles to Remove Uranyl Ions from Aqueous Phase

    SciTech Connect (OSTI)

    Wang, L.; Yang, Z.; Gao, J.; Xu, K.; Gu, H.; Xu, B.; Zhang, B.; Zhang, X.

    2007-03-20

    Using tetraethyl-3-amino-propane-1,1-bisphosphonate (BP) as the functional molecule, we functionalized Fe3O4 magnetic nanoparticles via dopamine (DA) linkage to create a system with an Fe3O4-DA-BP nanostructure, which possesses high specificity for removing uranyl ions from water or blood. This work demonstrates that magnetic nanoparticles, combined with specific receptor-ligand interactions, promise a sensitive and rapid platform for the detection, recovery, and decorporation of radioactive metal toxins from biological environment.

  7. Dissociation of the cellulosome of Clostridium thermocellum in the presence of ethylenediaminetetraacetic acid occurs with the formation of truncated polypeptides

    SciTech Connect (OSTI)

    Choi, Sang Ki; Ljungdahl, L.G.

    1996-04-16

    The cellulosome of Clostridium thermocellum JW20 consists of 14-26 different polypeptides as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The intact cellulosome hydrolyzes crystalline cellulose in the presence of Ca{sup 2+} and thiols. This activity is inhibited by ethylenediaminetetraacetic acid (EDTA). Ca is incorporated into the cellulosome and is tightly bound as demonstrated using {sup 45}Ca added to the growth medium. Upon incubation in 50 mM Tris (pH 7.5), 0.1 M NaCl, and 5 mM EDTA at 37{degrees}C, C is released form the cellulosome, which disintegrates into polypeptides. The SDS-PAGE pattern of cellulosomal polypeptides is remarkably different after the EDTA treatment when compared to this pattern of untreated cellulosomes. Polypeptide bands corresponding to molecular masses of 160, 98, 76, and 54 kDa disappear, and new bands of masses 150, 132, 91, 71, 57,and 46 kDa appear. N-terminal analyses of the 98, 76, 91, and 71 kDa polypeptides show that the 91 and 71 kDa polypeptides are truncated products of the 98 and 76 kDa polypeptides, respectively. The 76 and 71 kDa polypeptides correspond to CelS. The 71 kDa polypeptide is formed from the 76 kDa polypeptide during the EDTA treatment, by a cleavage that occurs at asparagine residue 681. It involves the removal of 60 amino acid residues from the C-terminal end. All catalytic subunits so far characterized contain an asparagine residue corresponding to residue 681 of CelS. This residue is part of the conserved duplicated region found in catalytically active subunits, and it is postulated that several of these subunits also are truncated by the EDTA treatment. The polypeptides truncated by the EDTA treatment had reduced Ca binding capacities compared to their native subunits, indicating a Ca-binding site within the conserved duplicated region. 63 refs., 7 figs., 2 tabs.

  8. Effect of dust-charge fluctuations on dust acoustic solitary waves in an inhomogeneous dusty plasma with nonextensive electrons

    SciTech Connect (OSTI)

    El-Labany, S. K.; Selim, M. M. E-mail: mselim2000@yahoo.com; Al-Abbasy, O. M.; El-Bedwehy, N. A.

    2015-02-15

    The effects of adiabatic dust grain charge fluctuation and inhomogeneity on the nonlinear properties of dust acoustic (DA) solitary waves are studied. The plasma under consideration is a hot magnetized dusty plasma consisting of negatively charged dust particles, Boltzmann ions, and nonextensive electrons. A modified Zakharov-Kusnetsov equation, which admits a solitary wave solution, is derived using the reductive perturbation theory. It is found that the charge fluctuation of the dust grain modifies the nature of DA solitary structures. The numerical results may be useful to understand phenomena in laboratory and astrophysical plasmas.

  9. Revised Manuscript

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Rev. 82 (1951) 305, DA12 1951RO16 W.D. Roseborough, J.J.G. McCue, W.M. Preston and C. Goodman, Phys. Rev. 83 (1951) 1133 1951YA1A Yaffe and Stevens, Can. J. Phys. 29 (1951) 186;...

  10. Virginia Senate Approves Budget Deal to Include Money for FEL (Daily Press)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    | Jefferson Lab Virginia Senate Approves Budget Deal to Include Money for FEL (Daily Press) External Link: http://articles.dailypress.com/2012-04-18/news/dp-nws-general-assembly-budget-da... By jlab_admin on Wed, 2012-04-18

  11. A thiocyanate hydrolase of Thiobacillus thioparus. A novel enzyme catalyzing the formation of carbonyl sulfide from thiocyanate

    SciTech Connect (OSTI)

    Katayama, Y.; Narahara, Y.; Inoue, Y.; Amano, F.; Kanagawa, T.; Kuraishi, H. (Faculty of Agriculture, Tokyo University of Agriculture and Technology, (Japan))

    1992-05-05

    A thiocyanate hydrolase that catalyzes the first step in thiocyanate degradation was purified to homogeneity from Thiobacillus thioparus, an obligate chemolithotrophic eubacterium metabolizing thiocyanate to sulfate as an energy source. The thiocyanate hydrolase was purified 52-fold by steps involving ammonium sulfate precipitation, DEAE-Sephacel column chromatography, and hydroxylapatite column chromatography. The enzyme hydrolyzed 1 mol of thiocyanate to form 1 mol of carbonyl sulfide and 1 mol of ammonia as follows: SCN- + 2H2O----COS + NH3 + OH-. This is the first report describing the hydrolysis of thiocyanate to carbonyl sulfide by an enzyme. The enzyme had a molecular mass of 126 kDa and was composed of three different subunits: alpha (19 kDa), beta (23 kDa), and gamma (32 kDa). The enzyme exhibited optimal activities at pH 7.5-8.0 and at temperatures ranging from 30 to 40 degrees C. The Km value for thiocyanate was approximately 11 mM. Immunoblot analysis with polyclonal antibodies against the purified enzyme suggested that it was induced in T. thioparus cells when the cells were grown with thiocyanate.

  12. OneTouch 4.0 Scanned Documents

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ".' .-.' .; . " c . ':-, A Publication of the Savannah River Ecology Laboratory National Environmental Research Park Program United States Department of Energy , ' , ' : '.- -; , .' : ..:,:...' ~, -~ .' '. "-:; 7A ,', , ' '.'; .~. . ",' " '~ ....;" . ',':' ._-~ ,... : . .... .~ <: ;:~,~.:,: :r. o * i * ~' . ,'." ..... , ~' J;i1'i~ '. J~. , " ', ' "" ,;; ;t! :':;J 'I1I$):i'iR tt ,WJ,S( ' (~,,!~d;;a~ an ,' account of.WQrk ; '*

  13. Crabbed Waist Collisions in DAFNE and Super-B Design

    SciTech Connect (OSTI)

    Raimondi, P.; Alesini, D.; Biagini, M.E.; Biscari, C.; Boni, R.; Boscolo, M.; Bossi, F.; Buonomo, B.; Clozza, A.; Delle Monache, G.O.; Demma, T.; Di Pasquale, E.; Di Pirro, G.; Drago, A.; Gallo, A.; Ghigo, A.; Guiducci, S.; Ligi, C.; Marcellini, F.; Mazzitelli, Giovanni; Milardi, C.; /Frascati /Orsay, LAL /CERN /Rome III U. /Rome U. /Novosibirsk, IYF /KEK, Tsukuba /INFN, Pisa /INFN, Cosenza /SLAC /Frascati

    2011-11-02

    The new idea of increasing the luminosity of a collider with crab waist collisions and first experimental results from the DA{Phi}NE {Phi}-Factory at LNF, Frascati, using this concept are presented. Consequences for the design of future factories will be discussed. An outlook to the performance reach with crab waist collisions is given, with emphasis on future B Factories.

  14. QER- Comment of Christopher Fee

    Broader source: Energy.gov [DOE]

    Until you stop subsidizing and worshipping at the altar of Big Energy, all this "We the people" crapola is just that. We no longer have. Democracy, Mr. Reed, because our government is owned by heartless men with big pockets. Sent from my iPhone. La dee freakin' da.

  15. Microsoft PowerPoint - ARM2008_poster_mbc_raf [Compatibility...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    d W t V V i bilit N Cl d M d Aerosol and Water Vapor Variability Near Clouds ... by t e Upg aded SG a a da 1 2 3 4 4 Richard Ferrare 1 David Turner 2 Marian Clayton 3 Rob Newsom 4 ...

  16. Aquantis Ocean Current Turbine Development Project Report

    SciTech Connect (OSTI)

    Fleming, Alex J.

    2014-08-23

    The Aquantis® Current Plane (“C-Plane”) technology developed by Dehlsen Associates, LLC (DA) and Aquantis, Inc. is an ocean current turbine designed to extract kinetic energy from ocean currents. The technology is capable of achieving competitively priced base-load, continuous, and reliable power generation from a source of renewable energy not before possible in this scale or form.

  17. Mapping and Assessment of the United States Ocean Wave Energy...

    Open Energy Info (EERE)

    Download Full Report URL: http:en.openei.orgdatasetsdataset6b40f428-2af0-40b3-8a53-0c32c7e35973resource9bfc4b34-78a1-4da9-8928-48a1f72ee8e8downloadmappingandassessmentofth...

  18. A=12C (1975AJ02)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    1973TR1B, 1973UL1B, 1974AR1G, 1974BE1R, 1974BO2K, 1974DA1N, 1974LA1J, 1974LA1K, 1974PA1E, 1974SC1F, 1974SN1B, 1974TO1C, 1974WI1F, 1975FA1H, 1975TA1E). Muon and neutrino...

  19. "RSE Table N5.2. Relative Standard Errors for Table N5.2;...

    U.S. Energy Information Administration (EIA) Indexed Site

    Standard Errors for Table N5.2;" " Unit: Percents." ,,"S e l e c t e d","W o o d","a n d","W o o d -","R e l a t e d","P r o d u c t s" ,,,,,"B i o m a s s" ,,,,,,"Wood Residues" ...

  20. Table 3.6 Selected Wood and Wood-Related Products in Fuel...

    U.S. Energy Information Administration (EIA) Indexed Site

    Column: Energy Sources;" " Unit: Trillion Btu." ,,"S e l e c t e d","W o o d","a n d","W o o d -","R e l a t e d","P r o d u c t s" ,,,,,"B i o m a s s" ,,,,,,"Wood Residues" ...

  1. Table N5.2. Selected Wood and Wood-Related Products in Fuel...

    U.S. Energy Information Administration (EIA) Indexed Site

    Column: Energy Sources;" " Unit: Trillion Btu." ,,"S e l e c t e d","W o o d","a n d","W o o d -","R e l a t e d","P r o d u c t s" ,,,,,"B i o m a s s" ,,,,,,"Wood Residues" ...

  2. ARM - Publications: Science Team Meeting Documents

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Using ARM Data - Development of a Case Study Data Set Starr, D.(a), Demoz, B.(b), Wang, Y.(c), Lin, R.-F.(d), Lare, A.(e), Mace, G.(f), Poellot, M.(g), Sassen, K.(h), and...

  3. Papers Published - April 1, 1997 - March 31, 1998

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    in 58Ni + Cu collisions at E 1.97A GeV E.L. Hjort, S. Albergo, F. Bieser, F.P. Brady, Z. Caccia, D.A. Cebra, A.D. Chacon, J.L. Chance, Y. Choi, S. Costa, J.B. Elliott,...

  4. A=19O (1983AJ01)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    83AJ01) (See Energy Level Diagrams for 19O) GENERAL: See (1978AJ03) and Table 19.1 Table of Energy Levels (in PDF or PS). Shell model: (1977GR16, 1979DA15, 1980KU05, 1982KI02)....

  5. Three dimensional dust-acoustic solitary waves in an electron depleted dusty plasma with two-superthermal ion-temperature

    SciTech Connect (OSTI)

    Borhanian, J.; Shahmansouri, M.

    2013-01-15

    A theoretical investigation is carried out to study the existence and characteristics of propagation of dust-acoustic (DA) waves in an electron-depleted dusty plasma with two-temperature ions, which are modeled by kappa distribution functions. A three-dimensional cylindrical Kadomtsev-Petviashvili equation governing evolution of small but finite amplitude DA waves is derived by means of a reductive perturbation method. The influence of physical parameters on solitary wave structure is examined. Furthermore, the energy integral equation is used to study the existence domains of the localized structures. It is found that the present model can be employed to describe the existence of positive as well as negative polarity DA solitary waves by selecting special values for parameters of the system, e.g., superthermal index of cold and/or hot ions, cold to hot ion density ratio, and hot to cold ion temperature ratio. This model may be useful to understand the excitation of nonlinear DA waves in astrophysical objects.

  6. Remedial investigation concept plan for the groundwater operable units at the chemical plant area and the ordnance works area, Weldon Spring, Missouri

    SciTech Connect (OSTI)

    1999-07-15

    The U.S. Department of Energy (DOE) and the U.S. Department of the Army (DA) are conducting cleanup activities at two properties--the DOE chemical plant area and the DA ordnance works area (the latter includes the training area)--located in the Weldon Spring area in St. Charles County, Missouri. These areas are on the National Priorities List (NPL), and cleanup activities at both areas are conducted in accordance with the Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA), as amended. DOE and DA are conducting a joint remedial investigation (RI) and baseline risk assessment (BRA) as part of the remedial investigation/feasibility study (RI/FS) for the groundwater operable units for the two areas. This joint effort will optimize further data collection and interpretation efforts and facilitate overall remedial decision making since the aquifer of concern is common to both areas. A Work Plan issued jointly in 1995 by DOE and the DA discusses the results of investigations completed at the time of preparation of the report. The investigations were necessary to provide an understanding of the groundwater system beneath the chemical plant area and the ordnance works area. The Work Plan also identifies additional data requirements for verification of the evaluation presented.

  7. Subunit composition and glycosidic activities of the cellulase complex from Clostridium thermocellum JW20

    SciTech Connect (OSTI)

    Kohring, S.; Mayer, F. ); Wiegel, J. )

    1990-12-01

    The subunit composition of the extracellular complex from Clostridium thermocellum was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Twenty-six bands, representing proteins with apparent molecular sizes ranging from 37,500 to 185,000 Da, could be detected by silver staining. Endoglucanase activity was exhibited in 15 of 26 bands and 13 showed xylanase activity. In 8 of the 26 bands, both activities could be found. As minor activities, {beta}-glucosidase, {beta}-xylosidase, {beta}-galactosidase, and {beta}-mannosidase activities could be demonstrated in the cellulase complex. Upon measuring the release of para-nitrophenol (PNP) from PNP-cellobioside and determining the amount of glucose formed, the presence of exoglucanase activity was indicated. Upon glycoprotein staining of SDS-polyacrylamide gels, 14 of the 26 bands reacted positive, indicating the glycoprotein nature of the respective proteins. Four proteins (apparent molecular sizes, 58,000, 72,500, 94,000, and 110,000 Da) could be enriched from the originally bound cellulase complex by preparative SDS-PAGE. The two smaller proteins exhibited xylanase activity, whereas the 94,000-Da protein had endo- and exoglucanase activity, and the 110,000-Da protein degraded PNP-pyranosides.

  8. A=18F (1983AJ01)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    1979DA15, 1980GO01, 1980KU05, 1980MA18, 1981EL1D, 1981ER03, 1981GR06, 1982KI02). Cluster, collective and deformed models: (1977BU22, 1978BU03, 1978PI1E, 1978SA15,...

  9. A=18O (1983AJ01)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    1979DA15, 1979WU06, 1980GO01, 1980KU05, 1980MA18, 1981EL1D, 1982KI02, 1982OL01). Cluster, collective and deformed models: (1977BU22, 1978BU03, 1978CH26, 1978PI1E,...

  10. 9Be Cross Section

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    S-factor Ecm 0.16 - 1.87 S(E) X4 01242012 2011GI05 9Be(, n): for n1 0.3 - 7.9 linear scale, log scale 06182012 1968DA05 9Be(, n): excitation function at 0...

  11. Recovery of dopamine transporters with methamphetamine detoxification is not linked to changes in dopamine release

    SciTech Connect (OSTI)

    Volkow, Nora D.; Wang, Gene-Jack; Smith, Lisa; Fowler, Joanna S.; Telang, Frank; Logan, Jean; Tomasi, Dardo

    2015-07-21

    Metamphetamine’s widepread abuse and concerns that it may increase Parkinson’s disease led us to assess if the reported loss of dopamine transporters (DAT) in methamphetamine abusers (MA) reflected damage to dopamine neurons. Using PET with [11C]cocaine to measure DAT, and with [11C]raclopride to measure dopamine release (assessed as changes in specific binding of [11C]raclopride between placebo and methylphenidate), which was used as marker of dopamine neuronal function, we show that MA (n=16), tested during early detoxification, had lower DAT (20-30%) but overall normal DA release in striatum (except for a small decrease in left putamen), when compared to controls (n=15). In controls, DAT were positively correlated with DA release (higher DAT associated with larger DA increases), consistent with DAT serving as markers of DA terminals. In contrast, MA showed a trend for a negative correlation (p=0.07) (higher DAT associated with lower DA increases), consistent with reduced DA re-uptake following DAT downregulation. MA who remained abstinent nine-months later (n=9) showed significant increases in DAT (20%) but methylphenidate-induced dopamine increases did not change. In contrast, in controls, DAT did not change when retested 9 months later but methylphenidate-induced dopamine increases in ventral striatum were reduced (p=0.05). Baseline D2/D3 receptors in caudate were lower in MA than in controls and did not change with detoxification, nor did they change in the controls upon retest. The loss of DAT in the MA, which was not associated with a concomitant reduction in dopamine release as would have been expected if DAT loss reflected DA terminal degneration; as well as the recovery of DAT after protracted detoxification, which was not associated with increased dopamine release as would have been expected if DAT increases reflected terminal regeneration, indicate that the loss of DAT in these MA does not reflect degeneration of dopamine terminals.

  12. Recovery of dopamine transporters with methamphetamine detoxification is not linked to changes in dopamine release

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Volkow, Nora D.; Wang, Gene-Jack; Smith, Lisa; Fowler, Joanna S.; Telang, Frank; Logan, Jean; Tomasi, Dardo

    2015-07-21

    Metamphetamine’s widepread abuse and concerns that it may increase Parkinson’s disease led us to assess if the reported loss of dopamine transporters (DAT) in methamphetamine abusers (MA) reflected damage to dopamine neurons. Using PET with [11C]cocaine to measure DAT, and with [11C]raclopride to measure dopamine release (assessed as changes in specific binding of [11C]raclopride between placebo and methylphenidate), which was used as marker of dopamine neuronal function, we show that MA (n=16), tested during early detoxification, had lower DAT (20-30%) but overall normal DA release in striatum (except for a small decrease in left putamen), when compared to controls (n=15).more » In controls, DAT were positively correlated with DA release (higher DAT associated with larger DA increases), consistent with DAT serving as markers of DA terminals. In contrast, MA showed a trend for a negative correlation (p=0.07) (higher DAT associated with lower DA increases), consistent with reduced DA re-uptake following DAT downregulation. MA who remained abstinent nine-months later (n=9) showed significant increases in DAT (20%) but methylphenidate-induced dopamine increases did not change. In contrast, in controls, DAT did not change when retested 9 months later but methylphenidate-induced dopamine increases in ventral striatum were reduced (p=0.05). Baseline D2/D3 receptors in caudate were lower in MA than in controls and did not change with detoxification, nor did they change in the controls upon retest. The loss of DAT in the MA, which was not associated with a concomitant reduction in dopamine release as would have been expected if DAT loss reflected DA terminal degneration; as well as the recovery of DAT after protracted detoxification, which was not associated with increased dopamine release as would have been expected if DAT increases reflected terminal regeneration, indicate that the loss of DAT in these MA does not reflect degeneration of dopamine terminals.« less

  13. Corotating solar wind structures and recurrent trains of enhanced diurnal variation in galactic cosmic rays

    SciTech Connect (OSTI)

    Yeeram, T.; Ruffolo, D.; Siz, A.; Kamyan, N.; Nutaro, T. E-mail: david.ruf@mahidol.ac.th E-mail: p_chang24@hotmail.com

    2014-04-01

    Data from the Princess Sirindhorn Neutron Monitor at Doi Inthanon, Thailand, with a vertical cutoff rigidity of 16.8 GV, were utilized to determine the diurnal anisotropy (DA) of Galactic cosmic rays (GCRs) near Earth during solar minimum conditions between 2007 November and 2010 November. We identified trains of enhanced DA over several days, which often recur after a solar rotation period (?27 days). By investigating solar coronal holes as identified from synoptic maps and solar wind parameters, we found that the intensity and anisotropy of cosmic rays are associated with the high-speed streams (HSSs) in the solar wind, which are in turn related to the structure and evolution of coronal holes. An enhanced DA was observed after the onset of some, but not all, HSSs. During time periods of recurrent trains, the DA was often enhanced or suppressed according to the sign of the interplanetary magnetic field B, which suggests a contribution from a mechanism involving a southward gradient in the GCR density, n, and a gradient anisotropy along B ?n. In one non-recurrent and one recurrent sequence, an HSS from an equatorial coronal hole was merged with that from a trailing mid-latitude extension of a polar coronal hole, and the slanted HSS structure in space with suppressed GCR density can account for the southward GCR gradient. We conclude that the gradient anisotropy is a source of temporary changes in the GCR DA under solar minimum conditions, and that the latitudinal GCR gradient can sometimes be explained by the coronal hole morphology.

  14. Structure of Glycerol Dehydratase Reactivase: A New Type of Molecular Chaperone

    SciTech Connect (OSTI)

    Liao, Der-Ing; Reiss, Lisa; Turner, Jr., Ivan; Dotson, Garry

    2010-03-08

    The function of glycerol dehydratase (GDH) reactivase is to remove damaged coenzyme B{sub 12} from GDH that has suffered mechanism-based inactivation. The structure of GDH reactivase from Klebsiella pneumoniae was determined at 2.4 {angstrom} resolution by the single isomorphous replacement with anomalous signal (SIR/AS) method. Each tetramer contains two elongated 63 kDa {alpha} subunits and two globular 14 kDa {beta} subunits. The {alpha} subunit contains structural features resembling both GroEL and Hsp70 groups of chaperones, and it appears chaperone like in its interactions with ATP. The fold of the {beta} subunit resembles that of the {beta} subunit of glycerol dehydratase, except that it lacks some coenzyme B12 binding elements. A hypothesis for the reactivation mechanism of reactivase is proposed based on these structural features.

  15. 13N

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    N β+-Decay Evaluated Data Measurements 1950HO01: 13N. 1953CH34: 13N. 1954GR66: 13N. 1955WI43: 13N. 1957DA08: 13N. 1957DE22: 13N. 1957NO17: 13N. 1958AR15: 13N. 1958DA09: 13N. 1960JA12: 13N; measured not abstracted; deduced nuclear properties. 1960KI02: 13N; measured not abstracted; deduced nuclear properties. 1965BO42: 13N; measured T1/2. 1965EB01: 13N; measured T1/2. 1968RI15: 13N; measured not abstracted; deduced nuclear properties. 1971GO40: 13N. 1973SIYS: 13N; measured T1/2. 1977AZ01: 13N;

  16. Ultrafast electron transfer at organic semiconductor interfaces: Importance of molecular orientation

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Ayzner, Alexander L.; Nordlund, Dennis; Kim, Do -Hwan; Bao, Zhenan; Toney, Michael F.

    2014-12-04

    Much is known about the rate of photoexcited charge generation in at organic donor/acceptor (D/A) heterojunctions overaged over all relative arrangements. However, there has been very little experimental work investigating how the photoexcited electron transfer (ET) rate depends on the precise relative molecular orientation between D and A in thin solid films. This is the question that we address in this work. We find that the ET rate depends strongly on the relative molecular arrangement: The interface where the model donor compound copper phthalocyanine is oriented face-on with respect to the fullerene C60 acceptor yields a rate that is approximatelymore » 4 times faster than that of the edge-on oriented interface. Our results suggest that the D/A electronic coupling is significantly enhanced in the face-on case, which agrees well with theoretical predictions, underscoring the importance of controlling the relative interfacial molecular orientation.« less

  17. Dust-acoustic solitary structures in a magnetized dusty plasma with two-temperature nonextensive electrons

    SciTech Connect (OSTI)

    Emamuddin, M.; Yasmin, S.; Asaduzzaman, M.; Mamun, A. A.

    2013-08-15

    The nonlinear propagation of dust-acoustic (DA) waves in an obliquely propagating magnetized dusty plasma, containing nonextensively distributed electrons of distinct temperatures (namely lower and higher temperature nonextensive electrons), negatively charged mobile dust grains, and Maxwellian ions, is rigorously studied and analyzed by deriving the Zakharov-Kuznetsov equation. It is found that the characteristics of the DA solitary waves (DASWs) are significantly modified by the external magnetic field, obliqueness of the system, nonextensivity of the electrons, electron temperature-ratios, and the respective number densities of two species of electrons. The results obtained from this analysis can be employed in understanding and treating the structures and the characteristics of DASWs both in laboratory and astrophysical plasma system.

  18. A=16O (1959AJ76)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    1959AJ76) (See the Energy Level Diagram for 16O) GENERAL: See also Table 16.3 [Table of Energy Levels] (in PDF or PS). Theory: See (DE54C, FL54A, HE55F, JA55A, MA55F, MA55O, SC55A, WI55F, EL56, FE56B, JA56C, KA56A, MO56, PE56A, RE56B, WI56C, EL57B, FE57D, GR57C, HE57B, RE57, TA57A, TO57A, CA58C, DA58A, DA58D, FE58A, FE58B, HA58B, MO58, RA58F, UM58, WI58G). 1. 12C(α, γ)16O Qm = 7.148 Resonant capture radiation to 16Og.s. is observed at Eα ~ 3.24 MeV, corresponding to the known J = 1- state at

  19. Optimization of dynamic aperture for hadron lattices in eRHIC

    SciTech Connect (OSTI)

    Jing, Yichao; Litvinenko, Vladimir; Trbojevic, Dejan

    2015-05-03

    The potential upgrade of the Relativistic Heavy Ion Collider (RHIC) to an electron ion collider (eRHIC) involves numerous extensive changes to the existing collider complex. The expected very high luminosity is planned to be achieved at eRHIC with the help of squeezing the beta function of the hadron ring at the IP to a few cm, causing a large rise of the natural chromaticities and thus bringing with it challenges for the beam long term stability (Dynamic aperture). We present our effort to expand the DA by carefully tuning the nonlinear magnets thus controlling the size of the footprints in tune space and all lower order resonance driving terms. We show a reasonably large DA through particle tracking over millions of turns of beam revolution.

  20. Regional

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    3 AÇORIANO ORIENTAL SEGUNDA-FEIRA, 5 DE MARÇO DE 2012 PUB Da Graciosa para a Índia graças à estação atmosférica Carlos está atualmente a trabalhar na estação atmosférica móvel instalada na Índia, a dois mil metros de altitude Estar no lugar certo na hora cer- ta pode mudar radicalmente a vida de uma pessoa. Foi isso que aconteceu ao graciosense Carlos Sousa, de 41 anos, que começou por ser trabalhador daconstrução civil antes de emigrar para os Es- tados Unidos da América. No

  1. The application of a logic framework for fatigue crack growth analyses to microstructural effects

    SciTech Connect (OSTI)

    Xu, J.G.; Liu, H.W.

    1995-12-31

    {Delta}K has been widely used to correlate da/dN data. The relation between da/dN and {Delta}K is usually found empirically. However, fatigue crack growth relations can also be derived theoretically. Three fatigue crack growth theories are derived for the state of small scale yielding and plane strain. These three theories constitute a logic framework useful for fatigue crack growth analyses. The application of the logic framework to the analyses of microstructural effects on fatigue crack growth is illustrated. The fatigue crack growth curve of 7075-T651 aluminum alloy has five distinct regions. A fatigue crack grows by crack-tip shear decohesion forming striations and by brittle fractures of particles followed by localized shear decohesion at these microcracks forming dimples. The logic framework helps to relate the fatigue crack growth behaviors in these five regions to the fractures of inclusions and to the resistance of grain boundaries and dispersoids to shear decohesion.

  2. Ultrafast electron transfer at organic semiconductor interfaces: Importance of molecular orientation

    SciTech Connect (OSTI)

    Ayzner, Alexander L.; Nordlund, Dennis; Kim, Do -Hwan; Bao, Zhenan; Toney, Michael F.

    2014-12-04

    Much is known about the rate of photoexcited charge generation in at organic donor/acceptor (D/A) heterojunctions overaged over all relative arrangements. However, there has been very little experimental work investigating how the photoexcited electron transfer (ET) rate depends on the precise relative molecular orientation between D and A in thin solid films. This is the question that we address in this work. We find that the ET rate depends strongly on the relative molecular arrangement: The interface where the model donor compound copper phthalocyanine is oriented face-on with respect to the fullerene C60 acceptor yields a rate that is approximately 4 times faster than that of the edge-on oriented interface. Our results suggest that the D/A electronic coupling is significantly enhanced in the face-on case, which agrees well with theoretical predictions, underscoring the importance of controlling the relative interfacial molecular orientation.

  3. Three-Stage Production Cost Modeling Approach for Evaluating the Benefits of Intra-Hour Scheduling between Balancing Authorities

    SciTech Connect (OSTI)

    Samaan, Nader A.; Milligan, Michael; Hunsaker, Matthew; Guo, Tao

    2015-07-30

    This paper introduces a Production Cost Modeling (PCM) approach to evaluate the benefits of intra-hour scheduling between Balancing Authorities (BAs). The system operation is modeled in a three-stage sequential manner: day ahead (DA)-hour ahead (HA)-real time (RT). In addition to contingency reserve, each BA will need to carry out “up” and “down” load following and regulation reserve capacity requirements in the DA and HA time frames. In the real-time simulation, only contingency and regulation reserves are carried out as load following is deployed. To model current real-time operation with hourly schedules, a new constraint was introduced to force each BA net exchange schedule deviation from HA schedules to be within NERC ACE limits. Case studies that investigate the benefits of moving from hourly exchange schedules between WECC BAs into 10-min exchange schedules under two different levels of wind and solar penetration (11% and 33%) are presented.

  4. Molecular Foundry

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    See the Foundry's full equipment list Organic and Macromolecular Synthesis Capabilities & Tools Instrument Scheduler Major Instruments and Capabilities AB SCIEX TF4800 MALDI TOF-TOF Mass Spectrometer This instrument is the tandem time-of-flight mass spectrometer systems, providing the excellent level of molecular mass coverage in the range of molecular masses 500 and 150,000 Da, high throughput, and confidence in both qualitative and quantitative analyses. The analyzer combines all of the

  5. 1

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    Global, Multi-Year Analysis of Clouds and Earth's Radiant Energy System Terra Observations and Radiative Transfer Calculations T.P. Charlock National Aeronautics and Space Administration Langley Research Center Hampton, Virginia F.G. Rose and D.A. Rutan Analytical Services and Materials Inc. Hampton, Virginia L.H. Coleman, T. Caldwell, and S. Zentz Systems and Applied Sciences Inc. Hampton, Virginia Introduction An extended record of the Terra Surface and Atmosphere Radiation Budget (SARB)

  6. 1

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    Validation of Satellite Retrieved Cloud Amounts Over the Continental United States with Automatic Sciences Research Center Ceilometer Data D.R. Doelling, D.N. Phan, and D.A. Spangenberg Analytical Services and Materials, Inc. Hampton, Virginia P. Minnis National Aeronautics and Space Administration - Langley Research Center Hampton, Virginia Introduction The National Aeronautics and Space Administration (NASA) Langley cloud and radiation retrieval products are produced near real time over the

  7. Fall

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    15! ! Fall Meeting of the Division of Nuclear Physics of the American Physical Society! Convention Center, Santa Fe, NM ! October 28-31, 2015! http://www.lanl.gov/dnp2015! Conference Coordinator: Tel - 630-416-3030! Complete Conference Coordinators, Inc.! 1260 Iroquois Ave., Suite 202! Naperville, Illinois 60563! Local Organizing Committee :! Joe Carlson! Vincenzo Cirigliano! Steven Clayton! Stefano Gandolfi! Marian Jandel! Christopher Lee! Hye Young Lee! Susan Seestrom! Cesar da Silva! Richard

  8. Enzymatic Digestibility and Pretreatment Degradation Products of

    Office of Scientific and Technical Information (OSTI)

    AFEX-Treated Hardwoods (Populus nigra) (Journal Article) | SciTech Connect Enzymatic Digestibility and Pretreatment Degradation Products of AFEX-Treated Hardwoods (Populus nigra) Citation Details In-Document Search Title: Enzymatic Digestibility and Pretreatment Degradation Products of AFEX-Treated Hardwoods (Populus nigra) Authors: Balan, Venkatesh ; Sousa, Leonardo da Costa ; Chundawat, Shishir P. S. ; Marshall, Derek ; Sharma, Lekh N. ; Chambliss, C. Kevin ; Dale, Bruce E. Publication

  9. Search for: All records | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    da Costa Sousa, Leonardo" Name Name ORCID Search Authors Type: All Book/Monograph Conference/Event Journal Article Miscellaneous Patent Program Document Software Manual Technical Report Thesis/Dissertation Subject: Identifier Numbers: Site: All Alaska Power Administration, Juneau, Alaska (United States) Albany Research Center (ARC), Albany, OR (United States) Albuquerque Complex - NNSA Albuquerque Operations Office, Albuquerque, NM (United States) Amarillo National Resource Center for

  10. Present Status of the DAFNE Upgrade And Perspectives

    SciTech Connect (OSTI)

    Milardi, C.; Alesini, D.; Biagini, M.E.; Biscari, C.; Boni, R.; Boscolo, M.; Bossi, F.; Buonomo, B.; Clozza, A.; Delle Monache, G.; Demma, T.; Di Pasquale, E.; Di Pirro, G.; Drago, A.; Gallo, A.; Ghigo, A.; Guiducci, S.; Ligi, C.; Marcellini, F.; Mazzitelli, G.; Murtas, F.; /Frascati /Novosibirsk, IYF /CERN /INFN, Cosenza /INFN, Rome /KEK, Tsukuba /Orsay, LAL /Rome U. /Pisa U. /INFN, Pisa /SLAC /Rome III U.

    2009-06-05

    The DA{Phi}NE collider has been recently upgraded in order to implement a new collision scheme based on large Piwinski angle and cancellation of the synchro-betatron resonances by means of electromagnetic sextupoles (Crab-Waist compensation). The novel approach has proved to be effective in improving beam-beam interaction and collider luminosity. The results and the measurements taken during commissioning as well as the perspectives for the SIDDHARTA run are presented and discussed.

  11. Dynamics of excitons and charges in organic materials and semiconductor

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    nanocrystals for optoelectronics | MIT-Harvard Center for Excitonics excitons and charges in organic materials and semiconductor nanocrystals for optoelectronics December 5, 2014 at 3pm/RLE Haus 36-428 Laurens D.A. Siebbeles Department of Chemical Engineering, Delft University of Technology Siebbeles01 Abstract: The seminar will report studies on excitons and charge carriers in conjugated polymers, covalent organic frameworks and semiconductor nanocrystals. These materials have fascinating

  12. System Plan Revision 5 + 6

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    Plan Revision 7 General Overview DaBrisha Smith (DOE/ORP) April 10, 2013 System Plan 101 * What is System Planning - A process used by organizations to design, analyze and define future operations possible outcomes. * What is the RRP System Plan - A summary-level document that describes how the technical, cost, and schedule operating scenario (Baseline Case) is integrated to meet the mission demands. - Describes how the RPP mission could be achieved based on a set of assumptions for each

  13. SU-E-T-84: Comparison of Three Different Systems for Patient-Specific Quality Assurance: Cranial Stereotactic Radiosurgery Using VMAT with Multiple Non Coplanar Arcs

    SciTech Connect (OSTI)

    Fusella, M; Fiandra, C; Giglioli, F; Ricardi, U; Ragona, R

    2014-06-01

    Purpose: Patient-specific quality assurance in volumetric modulated arc therapy (VMAT) brain stereotactic radiosurgery raises specific issues on dosimetric procedures, mainly represented by the small radiation fields associated with the lack of lateral electronic equilibrium, the need of small detectors and the high dose delivered. The purpose of the study is to compare three different dosimeters for pre-treatment QA. Methods: Nineteen patients (affected by neurinomas, brain metastases, and by meningiomas) were treated with VMAT plans computed on a Monte Carlo based TPS. Gafchromic films inside a slab phantom (GF), 3-D cylindrical phantom with two orthogonal diodes array (DA), and 3-D cylindrical phantom with a single rotating ionization chambers array (ICA), have been evaluated. The dosimeters are, respectively, characterized by a spatial resolution of: 0.4 (in our method), 5 and 2.5 mm. For GF we used a double channel method for calibration and reading protocol; for DA and ICA we used the 3-D dose distributions reconstructed by the two software sold with the dosimeters. With the need of a common system for analyze different measuring approaches, we used an in-house software that analyze a single coronal plane in the middle of the phantoms and Gamma values (2% / 2 mm and 3% / 3 mm) were computed for all patients and dosimeters. Results: The percentage of points with gamma values less than one was: 95.7% for GF, 96.8% for DA and 95% for ICA, using 3%/3mm criteria, and 90.1% for GF, 92.4% for DA and 92% for ICA, using 2% / 2mm gamma criteria. Tstudent test p-values obtained by comparing the three datasets were not statistically significant for both gamma criteria. Conclusion: Gamma index analysis is not affected by different spatial resolution of the three dosimeters.

  14. Modeling & Simulation publications

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    Modeling & Simulation » Modeling & Simulation Publications Modeling & Simulation publications Research into alternative forms of energy, especially energy security, is one of the major national security imperatives of this century. Get Expertise David Harradine Physical Chemistry and Applied Spectroscopy Email Josh Smith Chemistry Email The inherent knowledge of transformation has beguiled sorcerers and scientists alike. D.A. Horner, F. Lambert, J.D. Kress, and L.A. Collins,

  15. ELECTROCHEMICALLY-MODULATED SEPARATIONS FOR SAFEGUARDS MEASUREMENTS

    SciTech Connect (OSTI)

    Green, Michael A.; Arrigo, Leah M.; Liezers, Martin; Orton, Christopher R.; Douglas, Matthew; Peper, Shane M.; Schwantes, Jon M.; Hazelton, Sandra G.; Duckworth, Douglas C.

    2010-08-11

    A critical objective of materials accountability in safeguards is the accurate and timely analysis of fuel reprocessing streams to detect both abrupt and prolonged diversions of nuclear materials. For this reason both on-line nondestructive (NDA) and destructive analysis (DA) approaches are sought-after. Current methods for DA involve grab sampling and laboratory based column extractions that are costly, hazardous, and time consuming. While direct on-line gamma measurements of Pu are desirable, they are not possible due to contributions from other actinides and fission products. Researchers at Pacific Northwest National Laboratory are currently investigating electrochemically-modulated separation (EMS) as a straightforward, cost-effective technology for selective separation of Pu or U from aqueous reprocessing streams. The EMS selectivity is electrochemically controlled and results from the sorption of Pu4+ and U4+ redox states onto the anodized target electrode, allowing for selective accumulation of U or Pu from nitric acid streams to be turned “on” or “off.” It is envisioned that this technology can be utilized to isolate Pu for both NDA and DA analysis. For the NDA approach, rapid Pu analysis by gamma-ray spectroscopy could be performed after chemical clean-up of activation and fission products by EMS. Likewise, in the DA approach, EMS could be used to retain and concentrate the Pu in nanogram quantities on the electrode surface to be transported to the lab for analysis using high precision mass spectrometry. Due to the challenges associated with complex matrices, a systematic investigation of the redox-dependent accumulation of Pu using EMS was necessary, and results will be presented. Approaches to mitigate interelement effects using large surface area cells will also be discussed. The EMS chemistry and spectroscopy for Pu isolation and measurement will be presented, proof-of-principle measurements will be described, and the application of this approach for materials accountability will be discussed.

  16. Designer synthetic media for studying microbial-catalyzed biofuel production

    Office of Scientific and Technical Information (OSTI)

    Open Access Designer synthetic media for studying microbial- catalyzed biofuel production Xiaoyu Tang 1* , Leonardo da Costa Sousa 2 , Mingjie Jin 2 , Shishir PS Chundawat 2,3 , Charles Kevin Chambliss 4 , Ming W Lau 2 , Zeyi Xiao 5 , Bruce E Dale 2 and Venkatesh Balan 2* Abstract Background: The fermentation inhibition of yeast or bacteria by lignocellulose-derived degradation products, during hexose/pentose co-fermentation, is a major bottleneck for cost-effective lignocellulosic

  17. Research Highlight

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    New Method for Retrieving Cloud Heights from Satellite Data Download a printable PDF Submitter: Chang, F., Science Systems and Applications, Inc. Minnis, P., NASA - Langley Research Center Area of Research: Cloud Distributions/Characterizations Working Group(s): Cloud Properties Journal Reference: Chang F, P Minnis, B Lin, MM Khaiyer, R Palikonda, and DA Spangenberg. 2010. "A modified method for inferring cloud top height using GOES-12 imager 10.7- and 13.3-µm data." Journal of

  18. ARM - Publications: Science Team Meeting Documents

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Convective Triggering/Capping Inversions in the Southern Great Plains Cripe, D.G. (a) and Randall, D.A. (b), Colorado State University Eleventh Atmospheric Radiation Measurement (ARM) Science Team Meeting An algorithm for determining Generalized Convective Available Potential Energy (GCAPE) has been developed at Colorado State University. This particular algorithm differs from other CAPE-determining algorithms in that convective clouds are allowed to originate at multiple levels, and the effects

  19. Search for: All records | DOE PAGES

    Office of Scientific and Technical Information (OSTI)

    da Costa Sousa, Leonardo" Name Name ORCID Search Authors Type: All Accepted Manuscript Published Article Publisher's Accepted Manuscript Journal Name: Subject: Identifier Numbers: Research Org: Sponsoring Org: Publication Date: to Update Date: to Sort: Relevance (highest to lowest) Publication Date (newest first) Publication Date (oldest first) Close Clear All Find Switch to Detail View for this search DOE PAGES Search Results Page 1 of 1 Search for: All records 4 results for: All records

  20. Search for: All records | DOE PAGES

    Office of Scientific and Technical Information (OSTI)

    da Rocha, Ulisses Nunes" Name Name ORCID Search Authors Type: All Accepted Manuscript Published Article Publisher's Accepted Manuscript Journal Name: Subject: Identifier Numbers: Research Org: Sponsoring Org: Publication Date: to Update Date: to Sort: Relevance (highest to lowest) Publication Date (newest first) Publication Date (oldest first) Close Clear All Find Switch to Detail View for this search DOE PAGES Search Results Page 1 of 1 Search for: All records 1 results for: All records

  1. Lidar Comparison for GoAmazon 2014/15 Field Campaign Report

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    0 Lidar Comparison for GoAmazon 2014/15 Field Campaign Report HMJ Barbosa DA Gouveia B Barja E Landulfo April 2016 DISCLAIMER This report was prepared as an account of work sponsored by the U.S. Government. Neither the United States nor any agency thereof, nor any of their employees, makes any warranty, express or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or process disclosed, or represents that

  2. Identifying and Understanding Environment-Induced Crack propagation Behavior in Ni-based Superalloy INCONEL 617

    SciTech Connect (OSTI)

    Ma, Longzhou

    2012-11-30

    The nickel-based superalloy INCONEL 617 is a candidate material for heat exchanger applications in the next-generation nuclear plant (NGNP) system. This project will study the crack propagation process of alloy 617 at temperatures of 650°C-950°C in air under static/cyclic loading conditions. The goal is to identify the environmental and mechanical damage components and to understand in-depth the failure mechanism. Researchers will measure the fatigue crack propagation (FCP) rate (da/dn) under cyclic and hold-time fatigue conditions, and sustained crack growth rates (da/dt) at elevated temperatures. The independent FCP process will be identified and the rate-controlled sustained loading crack process will be correlated with the thermal activation equation to estimate the oxygen thermal activation energy. The FCP-dependent model indicates that if the sustained loading crack growth rate, da/dt, can be correlated with the FCP rate, da/dn, at the full time dependent stage, researchers can confirm stress-accelerated grain-boundary oxygen embrittlement (SAGBOE) as a predominate effect. Following the crack propagation tests, the research team will examine the fracture surface of materials in various cracking stages using a scanning electron microscope (SEM) and an optical microscope. In particular, the microstructure of the crack tip region will be analyzed in depth using high resolution transmission electron microscopy (TEM) and electron energy loss spectrum (EELS) mapping techniques to identify oxygen penetration along the grain boundary and to examine the diffused oxygen distribution profile around the crack tip. The cracked sample will be prepared by focused ion beam nanofabrication technology, allowing researchers to accurately fabricate the TEM samples from the crack tip while minimizing artifacts. Researchers will use these microscopic and spectroscopic results to interpret the crack propagation process, as well as distinguish and understand the environment or SAGBOE damage process under hold-time fatigue and sustained loading conditions

  3. CAPTURE DOCUMENT ORAUTEAM

    Office of Legacy Management (LM)

    DATA CAPTURE DOCUMENT ORAUTEAM ---- Dose Reconstruction ~v~:7 DISCOVERY AND REVIEW dA'~ Project for NIOSH The attached document may contain Privacy Act data. This information is protected by the Privacy Act, 5 U.S.C. §552a; disclosure to any third party without written consent of the individual to whom the information pertains is strictly prohibited. Data Capture Team or Other ORAU Team Member Capturing Data: Complete all information that applies to the data/document being submitted lor

  4. ackd290.tmp

    Office of Scientific and Technical Information (OSTI)

    Cap plasticity ' deformation A. F. Fosstm and ~w')Da430-osz~ models and compactive and dilatant pre-fadure J. T. Fredrich Sandia National Laboratories, Albuquerque, New Mexico 0 ABSTRACT: At low mean stresses, porous geomateriak faiI by shear Focalization, and at higher mean stresses, they undergo strain-hardening behavior. Cap plasticity models attempt to model this behavior using a pressure-dependent shear yield andlor shear limit-state envelope with a hardening or hardeningkoftening el-

  5. doe sc arm 15 070 CampaignT3LidarIPEN_v3_edited

    Office of Scientific and Technical Information (OSTI)

    70 Lidar Comparison for GoAmazon 2014/15 Field Campaign Report HMJ Barbosa DA Gouveia B Barja E Landulfo April 2016 CLIMATE RESEARCH FACILITY DISCLAIMER This report was prepared as an account of work sponsored by the U.S. Government. Neither the United States nor any agency thereof, nor any of their employees, makes any warranty, express or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or process

  6. Higher order nonlinear equations for the dust-acoustic waves in a dusty plasma with two temperature-ions and nonextensive electrons

    SciTech Connect (OSTI)

    Emamuddin, M.; Yasmin, S.; Mamun, A. A.

    2013-04-15

    The nonlinear propagation of dust-acoustic waves in a dusty plasma whose constituents are negatively charged dust, Maxwellian ions with two distinct temperatures, and electrons following q-nonextensive distribution, is investigated by deriving a number of nonlinear equations, namely, the Korteweg-de-Vries (K-dV), the modified Korteweg-de-Vries (mK-dV), and the Gardner equations. The basic characteristics of the hump (positive potential) and dip (negative potential) shaped dust-acoustic (DA) Gardner solitons are found to exist beyond the K-dV limit. The effects of two temperature ions and electron nonextensivity on the basic features of DA K-dV, mK-dV, and Gardner solitons are also examined. It has been observed that the DA Gardner solitons exhibit negative (positive) solitons for qq{sub c}) (where q{sub c} is the critical value of the nonextensive parameter q). The implications of our results in understanding the localized nonlinear electrostatic perturbations existing in stellar polytropes, quark-gluon plasma, protoneutron stars, etc. (where ions with different temperatures and nonextensive electrons exist) are also briefly addressed.

  7. CRYSTAL STRUCTURE OF CLOSTRIDIUM BOTULINUM NEUROTOXIN SEROTYPE B.

    SciTech Connect (OSTI)

    SWAMINATHAN,S.; ESWARAMOORTHY,S.

    2001-11-19

    The toxigenic strains of Clostridium botulinum produce seven serologically distinct types of neurotoxins labeled A - G (EC 3.4.24.69), while Clostridium tetani produces tetanus neurotoxin (EC 3.4.24.68). Botulinum and tetanus neurotoxins (BoNTs and TeNT) are produced as single inactive chains of molecular mass of approximately 150 kDa. Most of these neurotoxins are released after being cleaved into two chains, a heavy chain (HI) of 100 kDa and a light chain (L) of 50 kDa held together by an interchain disulfide bond, by tissue proteinases. BoNT/E is released as a single chain but cleaved by host proteinases [1]. Clostvidium botulinum neurotoxins are extremely poisonous proteins with their LD{sub 50} for humans in the range of 0.1 - 1 ng kg{sup -1} [2]. Botulinum neurotoxins are responsible for neuroparalytic syndromes of botulism characterized by serious neurological disorders and flaccid paralysis. BoNTs block the release of acetylcholine at the neuromuscular junction causing flaccid paralysis while TeNT blocks the release of neurotransmitters like glycine and {gamma}-aminobutyric acid (GABA) in the inhibitory interneurons of the spinal cord resulting in spastic paralysis. In spite of different clinical symptoms, their aetiological agents intoxicate neuronal cells in the same way and these toxins have similar structural organization [3].

  8. The Multi-Isotope Process Monitor: Multivariate Analysis of Gamma Spectra

    SciTech Connect (OSTI)

    Orton, Christopher R.; Rutherford, Crystal E.; Fraga, Carlos G.; Schwantes, Jon M.

    2011-10-30

    The International Atomic Energy Agency (IAEA) has established international safeguards standards for fissionable material at spent fuel reprocessing plants to ensure that significant quantities of nuclear material are not diverted from these facilities. Currently, methods to verify material control and accountancy (MC&A) at these facilities require time-consuming and resource-intensive destructive assay (DA). The time delay between sampling and subsequent DA provides a potential opportunity to divert the material out of the appropriate chemical stream. Leveraging new on-line nondestructive assay (NDA) techniques in conjunction with the traditional and highly precise DA methods may provide a more timely, cost-effective and resource efficient means for MC&A verification at such facilities. Pacific Northwest National Laboratory (PNNL) is developing on-line NDA process monitoring technologies, including the Multi-Isotope Process (MIP) Monitor. The MIP Monitor uses gamma spectroscopy and pattern recognition software to identify off-normal conditions in process streams. Recent efforts have been made to explore the basic limits of using multivariate analysis techniques on gamma-ray spectra. This paper will provide an overview of the methods and report our on-going efforts to develop and demonstrate the technology.

  9. Nonlinear dust acoustic waves in inhomogeneous four-component dusty plasma with opposite charge polarity dust grains

    SciTech Connect (OSTI)

    El-Taibany, W. F.

    2013-09-15

    The reductive perturbation technique is employed to investigate the propagation properties of nonlinear dust acoustic (DA) waves in a four-component inhomogeneous dusty plasma (4CIDP). The 4CIDP consists of both positive- and negative-charge dust grains, characterized by different mass, temperature, and density, in addition to a background of Maxwellian electrons and ions. The inhomogeneity caused by nonuniform equilibrium values of particle densities, fluid velocities, and electrostatic potential leads to a significant modification to the nature of nonlinear DA solitary waves. It is found that this model reveals two DA wave velocities, one slow, ?{sub s}, and the other is fast, ?{sub f}. The nonlinear wave evolution is governed by a modified Kortweg-de Vries equation, whose coefficients are space dependent. Both the two soliton types; compressive and rarefactive are allowed corresponding to ?{sub s}. However, only compressive soliton is created corresponding to ?{sub f}. The numerical investigations illustrate the dependence of the soliton amplitude, width, and velocity on the plasma inhomogeneities in each case. The relevance of these theoretical results with 4CIDPs observed in a multi-component plasma configurations in the polar mesosphere is discussed.

  10. Evaluation of the Dopamine Hypothesis of ADHD with PET Brain Imaging

    ScienceCinema (OSTI)

    Swanson, James [University of California, Irvine, California, United States

    2010-09-01

    The Dopamine (DA) Hypothesis of ADHD (Wender, 1971; Levy, 1990) suggests that abnormalities in the synaptic mechanisms of DA transmission may be disrupted, and specific abnormalities in DA receptors and DA transporters (DAT) have been proposed (see Swanson et al, 1998). Early studies with small samples (e.g., n = 6, Dougherty et al, 1999) used single photon emission tomography (SPECT) and the radioligand (123I Altropane) to test a theory that ADHD may be caused by an over expression of DAT and reported 'a 70% increase in age-corrected dopamine transporter density in patients with attention deficit hyperactivity disorder compared with healthy controls' and suggested that treatment with stimulant medication decreased DAT density in ADHD patients and corrected an underlying abnormality (Krause et al, 2000). The potential importance of these findings was noted by Swanson (1999): 'If true, this is a major finding and points the way for new investigations of the primary pharmacological treatment for ADHD (with the stimulant drugs - e.g., methylphenidate), for which the dopamine transporter is the primary site of action. The potential importance of this finding demands special scrutiny'. This has been provided over the past decade using Positron Emission Tomography (PET). Brain imaging studies were conducted at Brookhaven National Laboratory (BNL) in a relatively large sample of stimulant-naive adults assessed for DAT (11C cocaine) density and DA receptors (11C raclopride) availability. These studies (Volkow et al, 2007; Volkow et al, 2009) do not confirm the hypothesis of increased DAT density and suggest the opposite (i.e., decreased rather than increased DAT density), and follow-up after treatment (Wang et al, 2010) does not confirm the hypothesis that therapeutic doses of methylphenidate decrease DAT density and suggests the opposite (i.e., increased rather than decreased DAT density). The brain regions implicated by these PET imaging studies also suggest that a motivation deficit may contribute as much as an attention deficit to the manifestation of behaviors that underlie the symptoms of ADHD.

  11. General single phase wellbore flow model

    SciTech Connect (OSTI)

    Ouyang, Liang-Biao; Arbabi, S.; Aziz, K.

    1997-02-05

    A general wellbore flow model, which incorporates not only frictional, accelerational and gravitational pressure drops, but also the pressure drop caused by inflow, is presented in this report. The new wellbore model is readily applicable to any wellbore perforation patterns and well completions, and can be easily incorporated in reservoir simulators or analytical reservoir inflow models. Three dimensionless numbers, the accelerational to frictional pressure gradient ratio R{sub af}, the gravitational to frictional pressure gradient ratio R{sub gf}, and the inflow-directional to accelerational pressure gradient ratio R{sub da}, have been introduced to quantitatively describe the relative importance of different pressure gradient components. For fluid flow in a production well, it is expected that there may exist up to three different regions of the wellbore: the laminar flow region, the partially-developed turbulent flow region, and the fully-developed turbulent flow region. The laminar flow region is located near the well toe, the partially-turbulent flow region lies in the middle of the wellbore, while the fully-developed turbulent flow region is at the downstream end or the heel of the wellbore. Length of each region depends on fluid properties, wellbore geometry and flow rate. As the distance from the well toe increases, flow rate in the wellbore increases and the ratios R{sub af} and R{sub da} decrease. Consequently accelerational and inflow-directional pressure drops have the greatest impact in the toe region of the wellbore. Near the well heel the local wellbore flow rate becomes large and close to the total well production rate, here R{sub af} and R{sub da} are small, therefore, both the accelerational and inflow-directional pressure drops can be neglected.

  12. Identification of Intrinsic Order and Disorder in the DNA Repair Protein XPA

    SciTech Connect (OSTI)

    Iakoucheva, Lilia M.; Kimzey, Amy L.; Masselon, Christophe D.; Bruce, James E.; Garner, Ethan C.; Brown, Celeste J.; Dunker, A. K.; Smith, Richard D.; Ackerman, Eric J.

    2001-03-01

    The damage recognition protein XPA is required to recognize a wide variety of bulky lesions during nucleotide excision repair (NER). Independent NMR solution structures of a human XPA protein (hXPA) fragment comprising approximately one-third of the full-length protein, the minimal DNA-binding domain (MBD), revealed that ~30% of the molecule was structurally disordered. To better characterize structural features of XPA, we performed time-resolved trypsin proteolysis on active, full-length recombinant Xenopus XPA protein (xXPA). The resulting proteolytic fragments were analyzed by electrospray ionization interface coupled to a Fourier transform ion cyclotron resonance (ESI-FTICR) mass spectrometry, SDS-polyacrylamide gel electrophoresis (PAGE), and selected N-terminal sequence determinations. The mass spectrum of the full-length xXPA was consistent with the predicted sequence, 30922.02 vs. 30922.45 Da; respectively. Moreover, the mass spectrometric data allowed the assignment of multiple xXPA fragments not resolvable by SDS PAGE. Full-length xXPA exhibited aberrant mobility on SDS-PAGE with an apparent MW of ~40 kDa. To test predictions that a Glu-rich region (E70-E76) or other local regions of high charge were responsible for this ~40% aberrant SDS-PAGE mobility, the MW's of partial proteolytic fragments from ~5 to 25 kDa precisely determined by ESI-FTICR MS were correlated with their gel positions. Surprisingly, all tested partial tryptic fragments within this size-range exhibited 10-42% divergence between calculated MW and that estimated by SDS-PAGE, thus indicating the origin of anomalous migration of XPA is not localized. The computer program Predictor of Natural Disordered Regions (PONDR) correctly identified several regions of xXPA either sensitive or resistant to partial proteolysis, thereby indicating that disorder in XPA shares sequence features with other well-characterized intrinsically unstructured proteins.

  13. Nuclear Forensic Inferences Using Iterative Multidimensional Statistics

    SciTech Connect (OSTI)

    Robel, M; Kristo, M J; Heller, M A

    2009-06-09

    Nuclear forensics involves the analysis of interdicted nuclear material for specific material characteristics (referred to as 'signatures') that imply specific geographical locations, production processes, culprit intentions, etc. Predictive signatures rely on expert knowledge of physics, chemistry, and engineering to develop inferences from these material characteristics. Comparative signatures, on the other hand, rely on comparison of the material characteristics of the interdicted sample (the 'questioned sample' in FBI parlance) with those of a set of known samples. In the ideal case, the set of known samples would be a comprehensive nuclear forensics database, a database which does not currently exist. In fact, our ability to analyze interdicted samples and produce an extensive list of precise materials characteristics far exceeds our ability to interpret the results. Therefore, as we seek to develop the extensive databases necessary for nuclear forensics, we must also develop the methods necessary to produce the necessary inferences from comparison of our analytical results with these large, multidimensional sets of data. In the work reported here, we used a large, multidimensional dataset of results from quality control analyses of uranium ore concentrate (UOC, sometimes called 'yellowcake'). We have found that traditional multidimensional techniques, such as principal components analysis (PCA), are especially useful for understanding such datasets and drawing relevant conclusions. In particular, we have developed an iterative partial least squares-discriminant analysis (PLS-DA) procedure that has proven especially adept at identifying the production location of unknown UOC samples. By removing classes which fell far outside the initial decision boundary, and then rebuilding the PLS-DA model, we have consistently produced better and more definitive attributions than with a single pass classification approach. Performance of the iterative PLS-DA method compared favorably to that of classification and regression tree (CART) and k nearest neighbor (KNN) algorithms, with the best combination of accuracy and robustness, as tested by classifying samples measured independently in our laboratories against the vendor QC based reference set.

  14. The white dwarfs within 25 pc of the Sun: Kinematics and spectroscopic subtypes

    SciTech Connect (OSTI)

    Sion, Edward M.; McCook, George P.; Wasatonic, Richard; Myszka, Janine; Holberg, J. B.; Oswalt, Terry D. E-mail: george.mccook@villanova.edu E-mail: janine.myszka@villanova.edu E-mail: toswalt@fit.edu

    2014-06-01

    We present the fractional distribution of spectroscopic subtypes, range and distribution of surface temperatures, and kinematical properties of the white dwarfs (WDs) within 25 pc of the Sun. There is no convincing evidence of halo WDs in the total 25 pc sample of 224 WDs. There is also little to suggest the presence of genuine thick disk subcomponent members within 25 pc. It appears that the entire 25 pc sample likely belongs to the thin disk. We also find no significant kinematic differences with respect to spectroscopic subtypes. The total DA to non-DA ratio of the 25 pc sample is 1.8, a manifestation of deepening envelope convection, which transforms DA stars with sufficiently thin H surface layers into non-DAs. We compare this ratio with the results of other studies. We find that at least 11% of the WDs within 25 pc of the Sun (the DAZ and DZ stars) have photospheric metals that likely originate from accretion of circumstellar material (debris disks) around them. If this interpretation is correct, then it suggests the possibility that a similar percentage have planets, asteroid-like bodies, or debris disks orbiting them. Our volume-limited sample reveals a pileup of DC WDs at the well-known cutoff in DQ WDs at T {sub eff} ? 6000 K. Mindful of small number statistics, we speculate on its possible evolutionary significance. We find that the incidence of magnetic WDs in the 25 pc sample is at least 8% in our volume-limited sample, dominated by cool WDs. We derive approximate formation rates of DB and DQ degenerates and present a preliminary test of the evolutionary scenario that all cooling DB stars become DQ WDs via helium convective dredge-up with the diffusion tail of carbon extending upward from their cores.

  15. Mira Performance Boot Camp HPC Systems Overview 2015

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ALCF Systems: An Overview of the Blue Gene/Q and DA Systems, Storage, Software, and Other Notes William Scullin ALCF Catalyst Group Mira P erformance B oot C amp - 1 9 M ay 2 015 Building Blocks of the Universe 2 Mira P erformance B oot C amp - 1 9 M ay 2 015 Anatomy of a Blue Gene/Q (Not Ours) 3 cument for Review March 30, 2012 9:45 am 7948ch01.fm Blue Gene/Q hardware overview Figure 1-2 shows the primary hardware components of the Blue Gene/Q system. Figure 1-2 Blue Gene/Q hardware overview

  16. 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Overview of Atmospheric Radiation Measurement Satellite Cloud and Radiation Products from Langley Research Center R. Palikonda, M.M. Khaiyer, D.R. Doelling, J.K. Ayers, D.A. Spangenberg, M.L. Nordeen, and D.N. Phan Analytical Services and Materials, Inc. Hampton, Virginia P. Minnis and L. Nguyen National Aeronautics and Space Administration Langley Research Center Climate Science Branch Hampton, Virginia P.W. Heck CIMSS/University of Wisconsin-Madison Madison, Wisconsin R. Arduini, Q.Z. Trepte,

  17. March 2016 Most Viewed Documents for Chemistry | OSTI, US Dept of Energy,

    Office of Scientific and Technical Information (OSTI)

    Office of Scientific and Technical Information March 2016 Most Viewed Documents for Chemistry LITERATURE REVIEW OF BORIC ACID SOLUBILITY DATA Crapse, K.; Kyser, E. (2011) 342 Electrical conductivity measurements of aqueous electrolyte solutions at high temperatures and high pressures Ho, P.C.; Palmer, D.A. (1995) 331 Decomposition of calcium sulfate: a review of the literature. [62 refs] Swift, W M; Panek, A F; Smith, G W; Vogel, G J; Jonke, A A (1976) 175 Vapor-liquid equilibria for nitric

  18. April 2013 Most Viewed Documents for Chemistry | OSTI, US Dept of Energy,

    Office of Scientific and Technical Information (OSTI)

    Office of Scientific and Technical Information April 2013 Most Viewed Documents for Chemistry Science Subject Feed Lithium literature review: lithium's properties and interactions Jeppson, D.W.; Ballif, J.L.; Yuan, W.W.; Chou, B.E. (1978) 123 /> Electrical conductivity measurements of aqueous electrolyte solutions at high temperatures and high pressures Ho, P.C.; Palmer, D.A. (1995) 102 /> Vapor-liquid equilibria for nitric acid-water and plutonium nitrate-nitric acid-water solutions

  19. FY

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    FY 2008 FOIA - Request Log Closed/ No. Dated Date HQ ~~- l Date Rec'd or Dir. Finalized 001 02/01/08 03/11/08 Transfer ':'11 correspondence: m~mora~da, documents, reports, recor~s, statement~, ~~dits, IV' IA AI Ir"I:9 r._.-._ I." Vvl I 11VU "UII' n\,,( lists of names, applications, diskettes, ietters, calendars or diary logs, facsimile logs, call sheets, tape recordings, video. movie recordings, notes, chars, photographs, electronic mail and other documents sand things that refer

  20. Evolved strains of Scheffersomyces stipitis achieving high ethanol productivity on acid- and base-pretreated biomass hydrolyzate at high solids loading

    Office of Scientific and Technical Information (OSTI)

    Slininger et al. Biotechnology for Biofuels (2015) 8:60 DOI 10.1186/S13068-015-0239-6 Biotechnology for Biofuels RESEARCH ARTICLE Open Access Evolved strains of Scheffersomyces stipitis achieving high ethanol productivity on acid- and base-pretreated biomass hydrolyzate at high solids loading Patricia J Slininger1*, Maureen A Shea-Andersh1, Stephanie R Thompson1, Bruce S Dien1, Cletus P Kurtzman2, Venkatesh Balan3, Leonardo da Costa Sousa3, Nirmal Uppugundla3, Bruce E Dale3 and Michael A Cotta1

  1. GoAmazon 2014/15- Oxidation Flow Reactor Final Campaign Report

    Office of Scientific and Technical Information (OSTI)

    5 GoAmazon2014/15: Oxidation Flow Reactor Final Campaign Report JL Jimenez BB Palm DA Day P Campuzano-Jost W Hu March 2016 CLIMATE RESEARCH FACILITY DISCLAIMER This report was prepared as an account of work sponsored by the U.S. Government. Neither the United States nor any agency thereof, nor any of their employees, makes any warranty, express or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or

  2. Tuning the Formations of Metal-Organic Frameworks by Modification of

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ratio of Reactant, Acidity of Reaction System, and Use of a Secondary Ligand | Center for Gas SeparationsRelevant to Clean Energy Technologies | Blandine Jerome Tuning the Formations of Metal-Organic Frameworks by Modification of Ratio of Reactant, Acidity of Reaction System, and Use of a Secondary Ligand Previous Next List Qian Gao, Ya-Bo Xie, Jian-Rong Li, Da-Qiang Yuan, Audrey A. Yakovenko, Ji-Hong Sun, and Hong-Cai Zhou, Cryst. Growth Des., 2012, 12 (1), pp 281-288 DOI: 10.1021/cg201059d

  3. Technical Agenda

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Day 3 Technical Agenda September 10 - Wednesday 08:30-09:00 Uncertainty analysis D-A. Becker (GRS) Special Topics 09:00-9:30 HM-modeling and two-phase flow R. Wolters (TUCl) J. Rutquist (LBNL) 9:30-10:00 P&T in the context of waste management W. Bollingerfehr (DBE TEC) J. Mönig (GRS) 10:00-10:30 Report on discussion results of the hydrology group (separate micromeeting) J. Wolf (GRS) K. Kuhlman (SNL) 10:30-10:45 Break 10:45-11:15 NEA-Salt Club update J. Mönig, Chairman (GRS) 11:15-12:00

  4. Baseline risk assessment for groundwater operable units at the Chemical Plant Area and the Ordnance Works Area, Weldon Spring, Missouri

    SciTech Connect (OSTI)

    1999-07-14

    The U.S. Department of Energy (DOE) and the U.S. Department of the Army (DA) are evaluating conditions in groundwater and springs at the DOE chemical plant area and the DA ordnance works area near Weldon Spring, Missouri. The two areas are located in St. Charles County, about 48 km (30 mi) west of St. Louis. The 88-ha (217-acre) chemical plant area is chemically and radioactively contaminated as a result of uranium-processing activities conducted by the U.S. Atomic Energy Commission in the 1950s and 1960s and explosives-production activities conducted by the U.S. Army (Army) in the 1940s. The 6,974-ha (17,232-acre) ordnance works area is primarily chemically contaminated as a result of trinitrotoluene (TNT) and dinitrotoluene (DNT) manufacturing activities during World War II. This baseline risk assessment (BRA) is being conducted as part of the remedial investigation/feasibility study (RUFS) required under the Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) of 1980, as amended. The purpose of the BRA is to evaluate potential human health and ecological impacts from contamination associated with the groundwater operable units (GWOUs) of the chemical plant area and ordnance works area. An RI/FS work plan issued jointly in 1995 by the DOE and DA (DOE 1995) analyzed existing conditions at the GWOUs. The work plan included a conceptual hydrogeological model based on data available when the report was prepared; this model indicated that the aquifer of concern is common to both areas. Hence, to optimize further data collection and interpretation efforts, the DOE and DA have decided to conduct a joint RI/BRA. Characterization data obtained from the chemical plant area wells indicate that uranium is present at levels slightly higher than background, with a few concentrations exceeding the proposed U.S. Environmental Protection Agency (EPA) maximum contaminant level (MCL) of 20 {micro}g/L (EPA 1996c). Concentrations of other radionuclides (e.g., radium and thorium) were measured at back-ground levels and were eliminated from further consideration. Chemical contaminants identified in wells at the chemical plant area and ordnance works area include nitroaromatic compounds, metals, and inorganic anions. Trichloroethylene (TCE) and 1,2-dichloroethylene (1,2 -DCE) have been detected recently in a few wells near the raffinate pits at the chemical plant.

  5. PROTON RESOf\j ANCE SPECTRO~3COPY

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ( ( ( ( ( . :( ( ( ( [ assea::::;: $_ r PROTON RESOf\j ANCE SPECTRO~3COPY IN 28Si AND 30p JI" II RONALD OWEN NELSON JI ~jL======.~=~=n=====_= ___====~.======~~ - Triangle Unive\'"$~t!es Nuclear l.oboratGry Deportment of Physics Duke University 1983 PROWN REHNAN <E Sl?EcrR.OS coPY IN 2 8 8i AND 30 p by Ronald Owen Nel son Department of Phy si cs Duke U niv er si ty Da te: Approved: E. G. Bilpuch, Sllperv isor Dissertation sllbmitted in partial fulfillment of the requirements for

  6. X:\ARM_19~1\P139-154.WPD

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    dA / dN C N k ( 1 to 2 W/m 2 ) 40 3 (2 km) 3 Session Papers 147 (1) Evaluating Aerosol Indirect Effect Through Marine Stratocumulus Clouds Z. N. Kogan, Y. L. Kogan, and D. K. Lilly Cooperative Institute for Mesoscale Meteorological Studies University of Oklahoma Norman, Oklahoma Introduction During the last decade much attention has been focused on anthropogenic aerosols and their radiative influence on the global climate. Charlson et al. (1992) and Penner et al. (1994) have demonstrated that

  7. leeann_nersc_workshop.ppt

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Doubling
*me
for
sequence
databases
 is
currently
~18
months
 According
to
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Law,
doubling
 *me
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processor
speed
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 months.
 Time
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 memory
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 During
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 only
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latency
 only
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 * source:
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"Latency
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Bandwidth:


  8. Portable software for distributed readout controllers and event builders in FASTBUS and VME

    SciTech Connect (OSTI)

    Pordes, R.; Berg, D.; Berman, E.; Bernett, M.; Brown, D.; Constanta-Fanourakis, P.; Dorries, T.; Haire, M.; Joshi, U.; Kaczar, K.; Mackinnon, B.; Moore, C.; Nicinski, T.; Oleynik, G.; Petravick, D.; Sergey, G.; Slimmer, D.; Streets, J.; Votava, M.; White, V.

    1989-12-01

    We report on software developed as part of the PAN-DA system to support the functions of front end readout controllers and event builders in multiprocessor, multilevel, distributed data acquisition systems. For the next generation data acquisition system we have undertaken to design and implement software tools that are easily transportable to new modules. The first implementation of this software is for Motorola 68K series processor boards in FASTBUS and VME and will be used in the Fermilab accelerator run at the beginning of 1990. We use a Real Time Kernel Operating System. The software provides general connectivity tools for control, diagnosis and monitoring. 17 refs., 7 figs.

  9. UCRL-53311 Distribution Category UC-41 U C R L

    Office of Scientific and Technical Information (OSTI)

    311 Distribution Category UC-41 U C R L - - 5 3 3 1 1 DE83 0 0 8 4 3 9 Particle Size for Greatest Penetration of HEPA Filters- and Their True Efficiency R. A. da Roza Manuscript date: December 1,1982 DISCLAIMER This report was prepared as aa accouat of work spoasored by aa ageacy of the Uaited S u t e s Government. Neither the Unite? States Government aor aay ageacy thereof, aor aay of their employee*, makes aay warranty, CSI^N^M or implied, or assumes aay legal liability or rcspoaci- bilily for

  10. A Case Study of Lean Implementation at Sandia National Laboratories.

    Office of Scientific and Technical Information (OSTI)

    (Conference) | SciTech Connect A Case Study of Lean Implementation at Sandia National Laboratories. Citation Details In-Document Search Title: A Case Study of Lean Implementation at Sandia National Laboratories. Abstract not provided. Authors: de Luna, Raul ; Lopez, Mike R. ; Wan, Hung-da ; Chen, Frank F. Publication Date: 2013-02-01 OSTI Identifier: 1116099 Report Number(s): SAND2013-0908C 480106 DOE Contract Number: AC04-94AL85000 Resource Type: Conference Resource Relation: Conference:

  11. An Automated Implementation of On-shell Methods for One-Loop Amplitudes

    Office of Scientific and Technical Information (OSTI)

    (Journal Article) | SciTech Connect Journal Article: An Automated Implementation of On-shell Methods for One-Loop Amplitudes Citation Details In-Document Search Title: An Automated Implementation of On-shell Methods for One-Loop Amplitudes Authors: Berger, C.F. ; Bern, Z. ; Dixon, L.J. ; Febres Cordero, F. ; Forde, D. ; Ita, H. ; Kosower, D.A. ; Maitre, D. ; /MIT, LNS /Santa Barbara, KITP /SLAC /UCLA /Saclay Publication Date: 2008-04-11 OSTI Identifier: 927069 Report Number(s):

  12. Guide of Good Practices for Occupational Radiological Protection in Uranium Facilities

    Energy Savers [EERE]

    )*&%&!# (&!(+#+,& ! -"&.(#-.//01.2333 '45467$2333 89'!:&$!"#+8&$!";$/ "<7=>?@$2333 89'!:&$!"#+8&$!";$2 %A@<B$233/ 89'!:&$!"#+8&$!";$0 -?<?C>?@$233/ -"&$(#'!-'*- "#$%&!'(!"''%!)*+,-$,&.!('* ',,#)+-$'/+0!*+%$'0'"$,+0 )*'-&,-$'/!$/!#*+/$#1!(+,$0$-$&. );(;$-?DA@7C?E7$=F$&E?@5G '*&'$H'8I JA6BKE57=EL$-;8;$23MNM

  13. Feasibility study for remedial action for the groundwater operable units at the chemical plant area and the ordnance works area, Weldon Spring, Missouri

    SciTech Connect (OSTI)

    1999-07-15

    The U.S. Department of Energy (DOE) and the U.S. Department of Army (DA) are conducting an evaluation to identify the appropriate response action to address groundwater contamination at the Weldon Spring Chemical Plant (WSCP) and the Weldon Spring Ordnance Works (WSOW), respectively. The two areas are located in St. Charles County, about 48 km (30 rni) west of St. Louis. The groundwater operable unit (GWOU) at the WSCP is one of four operable units being evaluated by DOE as part of the Weldon Spring Site Remedial Action Project (WSSRAP). The groundwater operable unit at the WSOW is being evaluated by the DA as Operable Unit 2 (OU2); soil and pipeline contamination are being managed under Operable Unit 1 (OU1). Remedial activities at the WSCP and the WSOW are being conducted in accordance with the Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA). Consistent with DOE policy, National Environmental Policy Act (NEPA) values have been incorporated into the CERCLA process. A remedial investigation/feasibility study (RI/FS) work plan summarizing initial site conditions and providing site hydrogeological and exposure models was published in August of 1995 (DOE 1995). The remedial investigation (RI) and baseline risk assessment (BRA) have also recently been completed. The RI (DOE and DA 1998b) discusses in detail the nature, extent, fate, and transport of groundwater and spring water contamination. The BRA (DOE and DA 1998a) is a combined baseline assessment of potential human health and ecological impacts and provides the estimated potential health risks and ecological impacts associated with groundwater and springwater contamination if no remedial action were taken. This feasibility study (FS) has been prepared to evaluate potential options for addressing groundwater contamination at the WSCP and the WSOW. A brief description of the history and environmental setting of the sites is presented in Section 1.1, key information relative to the nature and extent of contamination is presented in Section 1.2, and the results of the BRA are summarized in Section 1.3. The objective of this FS is discussed in Section 1.4, and preliminary remediation goals are identified in Section 1.5. The organization of the remaining chapters of this FS is outlined in Section 1.6.

  14. DE-AC27-I1ORVI15051 Modification A009

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    I1ORVI15051 Modification A009 Page 2 of 6 A. The purpose of this modification is to make an equitable adjustment to contract cost relevant to transition cost and the stop work order dated November 27, 2009, and modify the period of performance as detailed below: Reference: 1. ATL Letter dated May 24, 2010, from J.G. Hwang, ATL, to D.A. Gallegos, ORP, "CLIN 1 TRANSITION COST OVERRUN PROPOSAL (Contract Number DE-AC27-1I0RV 1505 1) Background: The contract was awarded on November 20, 2009, and

  15. JF.!+

    Office of Legacy Management (LM)

    1 ' , : ,. Department of-Eik-~ ' Washington. DC 20585, " JF.!+ ; ;' jg' , The Horiorab1.e Paul DeLaney I 200 El University Avenue I Gainesville, Flori' da 32601 ..' " . Dear Mayor DeLaney: ",, 1 / I .I Secretary of Energy Hazel D'Leary has announced's new approach to openness in the Department of Energy-(DDE) and its communications with the public! In .', support,of this initiative, we are pleased to forward the encTosed information related to the formeruniversity of Florida site

  16. NATIONAL KAD CO. OF OHIO - HEALTH & SAFETY DIVISION

    Office of Legacy Management (LM)

    KAD CO. OF OHIO - HEALTH & SAFETY DIVISION NC) 1602 c ! 9 Hygionm or Medical Dapt. h 2 44 - - =.- Hour Sample Description 6 \ : ' _/ *. I I . ..$$$ +ri, I- .' i C "I. I I I I . 1 I * ,' z Analytical Cha4dA -K-F- Counting D&a: 4; 9 7.' __-__--__ ' T ..__ . . -~ -- --- ---_ . NATIONAL MAD CO. OP OHIO - HEALTH & SAFWY DIVISION N ? ,299 Industrial Hygiene or Medical D8pt. 1. H.#581kmph Nos. D8t8 Cobxted 3 +%ay Rtis Route to RHs Location Uaah-Rite CO- Type of Smpl8~nslyz8d for$m

  17. Characterization of supernumerary ring marker chromosomes by fluorescence in situ hybridization (FISH)

    SciTech Connect (OSTI)

    Blennow, E.; Nordenskjoeld, M. ); Asadi, E. ); Anneren, G.; Berggren, E.; Nordenskjoeld, M.

    1993-08-01

    Five cases with small supernumerary ring chromosomes are characterized at the molecular level. Routine chromosome banding analysis was insufficient for identification of the ring chromosomes, and none of them was DA/DAPI positive. Fluorescence in situ hybridization utilizing repetitive centromeric probes for all chromosomes has determined that one of these five ring chromosomes originates in each of chromosomes 4, 7, 8, 9, and 20. Chromosome painting with chromosome-specific libraries has confirmed this and excluded the involvement of additional chromosomes in the rearrangements. 30 refs., 3 figs., 2 tabs.

  18. Architecture and technology of 500 Msample/s feedback systems for control of coupled-bunch instabilities

    SciTech Connect (OSTI)

    Teytelman, Dmitry

    2000-03-30

    Feedback control of coupled-bunch instabilities presents many challenges. Control bandwidths up to 250 MHz are required to damp all of the unstable coupled-bunch modes in recent accelerators. A digital parallel-processing array with 80 DSPs has been developed to control longitudinal instabilities in PEP-II/ALS/DA NE machines. Here the authors present a description of the architecture as well as the technologies used to implement 500 Msample/s real-time control system with 2,000 FIR filtering channels. Algorithms for feedback control, data acquisition, and analysis are described and measurements from ALS are presented.

  19. TTW 11-9-06

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    9, 2006 WIPP Quick Facts (As of 11-8-06) 5,235 Shipments received since opening 43,621 Cubic meters of waste disposed 84,477 Containers disposed in the underground Veterans' Day November 11, 2006 The following comments are from just a sample of veterans that are part of the WIPP family. I'm a Vietnam Veteran...served at DaNang (1971-72) with the U.S. Air Force. Assigned to 366th Tactical Fighter Wing "The Gunfighters" (F- 4s). When I returned to the USA in December of '72, I recall a

  20. AMENDMENT OF SOLICITATION/MODIFICATION OF CONTRACT 11. CONTRACT ID CODE

    National Nuclear Security Administration (NNSA)

    l PAGE OF PAGES 1 I 3 2. AMENDMENT/MODIFICATION NO. See Block 16C 4. REQUISITION/PURCHASE REQ. NO. 15. PROJECT NO. (If applicable) 3. EFFECTIVE DA TE 0264 6. ISSUED BY CODE 05003 NNSA/Los Alamos Site Office U.S. Department of Energy NNSA/Los Alamos Site Office 3747 West Jemez Road Los Alamos NM 87544 8. NAME AND ADDRESS OF CONTRACTOR (No., street, county, State and ZIP Code) LOS ALAMOS NATIONAL SECURITY, LLC Attn: STEVE K. SHOOK P.O. BOX 1663, MS P222 LOS ALAMOS NM 875450001 CODE 175252894

  1. News Item

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    List 3010 INSITU 80+ Reactive Ion Etcher (tool referred to as the RIE by nanofab staff) AAPPTec Apex 396 Peptide Synthesizer AB Sciex TF4800 MALDI-TOF-TOF - Ideal for small molecules and (bio)polymers between 500 and 150,000 Da molecular weights ABM optical contact printer Agilent (Molecular Imaging) PicoPlus Scanning Probe Microscope Agilent 1100 series (ion trap) LC-MS-MS Mass spectrometer Agilent 1100 Series Agilent 1200 nanoHPLC System Agilent 1260 Infinity Agilent analytical HPLC Agilent

  2. Geochemical Behaviour of S, Cl and Fe in Silicate Melts/Glasses Simulating

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Natural Magmas | Stanford Synchrotron Radiation Lightsource Geochemical Behaviour of S, Cl and Fe in Silicate Melts/Glasses Simulating Natural Magmas Monday, March 26, 2012 - 11:00am SSRL Conference Room 137-322 G. Giuli, R. Alonso-Mori, E. Paris, P. Glatzel, S. Eeckhout, M. Carroll, School of Science and Technology, Geology Division, University of Camerino, Via G. III da Varano, 62032 Camerino; e-mail: The solubility behaviour of S and Cl in silicate magmas is a key to understand the

  3. Nitrogen Removal from Natural Gas

    Office of Scientific and Technical Information (OSTI)

    Nitrogen Removal from Natural Gas Phase II Draft Final Report Contract Number DE-AC21-95MC32199--02 Contract Period: July 29, 1996 - December 31, 1999 prepared by Membrane Technology and Research, Inc. 1360 Willow Road Menlo Park, CA 94025 December 22, 1999 for the U.S. Department of Energy Morgantown Energy Technology Center Morgantown, WV Contributors to this Report: K.A. Lokhandwala M.B. Ringer T.T. Su Z. He I. Pinnau J.G. Wijmans A. Morisato K. Amo A. Da Costa R.W. Baker R. Olsen H. Hassani

  4. Competing charge, spin, and superconducting orders in underdoped

    Office of Scientific and Technical Information (OSTI)

    YBa[subscript 2]Cu[subscript 3]O[subscript y] (Journal Article) | SciTech Connect YBa[subscript 2]Cu[subscript 3]O[subscript y] Citation Details In-Document Search Title: Competing charge, spin, and superconducting orders in underdoped YBa[subscript 2]Cu[subscript 3]O[subscript y] Authors: Hucker, M. ; Christensen, N.B. ; Holmes, A.T. ; Blackburn, E. ; Forgan, E.M. ; Liang, Ruixing ; Bonn, D.A. ; Hardy, W.N. ; Gutowski, O. ; Zimmermann, M.v. ; Hayden, S.M. ; Chang, J. [1] ; Denmark) [2] ;

  5. CASL - Mixing and non-stoichiometry in Fe-Ni-Cr-Zn-O spinel compounds:

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Density functional theory calculations Mixing and non-stoichiometry in Fe-Ni-Cr-Zn-O spinel compounds: Density functional theory calculations Mixing and non-stoichiometry in Fe-Ni-Cr-Zn-O spinel compounds: Density functional theory calculations D.A. Andersson and C.R. Stanek Materials Science and Technology Division Los Alamos National Laboratory Density functional theory (DFT) calculations have been employed to better understand the thermodynamic properties of AB2O4 (where A=Fe2+, Ni2+ or

  6. A

    Office of Legacy Management (LM)

    ;~'1 ) A / " & - fOL-J/ ~{Z.-- 3> . \ ABANDONMENT O~ILLED HOLES CENTRAL NEVADA TEST AREA IT LAS lIEGt\S unRARY PROPERTY OF U.S.GOVERNMENT 6NIX & SCISSON, INC. 5105 EAST 15th STRUT TULS .... OKLAHOMA 74112 MARCH 1973 LFENIX & SCISSON. INC. 300 L"'S VEG S BOULEVARD SOUTH L"'S VEG S. NEV"'DA 89101 L U. I. ATOMIC INBROY COlllolllIlON CONTUCT U(26-1I*18 DISCLAIMER Portions of this document may be illegible in electronic image products. Images are produced from the

  7. Publications by First Author | Photosynthetic Antenna Research Center

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Publications by First Author Publications by First Author A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | R | S | T | U | V | W | X | Y | Z A Adams NBP, Vasilev C, Brindley AA, and Hunter CN (2016) Nanomechanical and thermophoretic analyses of the nucleotide-dependent interactions between the AAA subunits of magnesium chelatase. Journal of the American Chemical Society. In press. Adams PG, Cadby AJ, Robinson B, Tsukatani Y, Tank M, Wen J, Blankenship RE, Bryant DA, and Hunter CN

  8. Research Highlight

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    More Like Shades of Gray: the Effects of Black Carbon in Aerosols Submitter: McComiskey, A. C., National Oceanic and Atmospheric Administration Area of Research: Aerosol Properties Working Group(s): Aerosol Life Cycle Journal Reference: Cappa CD, TB Onasch, P Massoli, DR Worsnop, TS Bates, ES Cross, P Davidovits, J Hakala, KL Hayden, BT Jobson, KR Kolesar, DA Lack, BM Lerner, SM Li, D Mellon, I Nuaaman, JS Olfert, T Petaja, PK Quinn, C Song, R Subramanian, EJ Williams, and RA Zaveri. 2012.

  9. March Events

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    March March 2016 Events March 2016 event highlights Mar 2 Wed 8:00 AM CoDA 2016, the Conference on Data Analysis Eldorado Hotel - 309 W. San Francisco St., Santa Fe, NM Join us for the Conference on Data Analysis, highlighting data-driven problems of interest to the Department of Energy. Mar 3 Thu 5:30 PM Women in Los Alamos History Los Alamos Golf Course Clubhouse Tietjen is a well-known speaker on the topic of gender issues in the work place, and a motivational speaker for young women working

  10. Search for: All records | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    Switch to Detail View for this search SciTech Connect Search Results Page 1 of 1 Search for: All records Creators/Authors contains: "Wang, Da-Neng" × Sort by Relevance Sort by Date (newest first) Sort by Date (oldest first) Sort by Relevance « Prev Next » Everything3 Electronic Full Text1 Citations2 Multimedia0 Datasets0 Software0 Filter Results Filter by Subject membranes (2) architecture (1) binding energy (1) biochemistry (1) biology (1) biophysics (1) clostridium (1) crystal

  11. Last Chance to Take JLab SAF130B Design Authority | Jefferson Lab

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Last Chance to Take JLab SAF130B Design Authority March 9 is Last Chance to Take JLab SAF130B Design Authority Training for Some Time Have you ever considered or been interested in becoming a Design Authority at Jefferson Lab? Or do you have an employee who could benefit from this training? If so, or even if you would just like to learn what a DA does: sign up now for the (SAF130B) Pressure Systems Safety Design Authority training session scheduled for Wednesday, March 9. The class, led by Dave

  12. Data mining for ontology development.

    SciTech Connect (OSTI)

    Davidson, George S.; Strasburg, Jana; Stampf, David; Neymotin,Lev; Czajkowski, Carl; Shine, Eugene; Bollinger, James; Ghosh, Vinita; Sorokine, Alexandre; Ferrell, Regina; Ward, Richard; Schoenwald, David Alan

    2010-06-01

    A multi-laboratory ontology construction effort during the summer and fall of 2009 prototyped an ontology for counterfeit semiconductor manufacturing. This effort included an ontology development team and an ontology validation methods team. Here the third team of the Ontology Project, the Data Analysis (DA) team reports on their approaches, the tools they used, and results for mining literature for terminology pertinent to counterfeit semiconductor manufacturing. A discussion of the value of ontology-based analysis is presented, with insights drawn from other ontology-based methods regularly used in the analysis of genomic experiments. Finally, suggestions for future work are offered.

  13. Sensor/source electrometer circuit

    SciTech Connect (OSTI)

    Hughes, W.J.

    1991-12-31

    A multiple decade electrometer circuit is claimed which can measure low input currents or act as a current source and is comprised of a microprocessor controlled digital to analog converters to derive individual decades. A plurality of decades are created by multiple D-A voltage sources which generate electrometer currents through scaled resistors. After a first series of decades of current are successively produced, the converters are 10 cycled to generate current through new resistors scaled to produce another series decades of current. In this manner, the electrometer circuit generates or senses a plurality of decades of current without significant scale change.

  14. Search for: All records | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    Switch to Detail View for this search SciTech Connect Search Results Page 1 of 1 Search for: All records Creators/Authors contains: "da Rocha, Ulisses Nunes" × Sort by Relevance Sort by Date (newest first) Sort by Date (oldest first) Sort by Relevance « Prev Next » Everything1 Electronic Full Text1 Citations0 Multimedia0 Datasets0 Software0 Filter Results Filter by Subject basic biological sciences (1) Filter by Author Brodie, Eoin L. (1) Estera, Katerina (1) Firestone, Mary (1) He,

  15. Searching

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Searching for Dark Matter Searches with MiniBooNE Presented to the FNAL PAC Dec 16, 2013 The MiniBooNE Collaboration R. Dharmapalan, & I. Stancu University of Alabama, Tuscaloosa, AL 35487 R. A. Johnson, & D.A. Wickremasinghe University of Cincinnati, Cincinnati, OH 45221 R. Carr, G. Karagiorgi, & M. H. Shaevitz Columbia University; New York, NY 10027 B.C. Brown, F.G. Garcia , R. Ford, T. Kobilarcik, W. Marsh, C. D. Moore, D. Perevalov, & W. Wester Fermi National Accelerator

  16. Supporting Advanced Scientific Computing Research * Basic Energy Sciences * Biological

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ESCC,
Salt
Lake
City
 Steve
Co6er,
Dept
Head

 steve@es.net

 Lawrence
Berkeley
NaDonal
Lab
 Outline
 * Staff
Updates
 * Network
Update
 * Advanced
Networking
IniDaDve
 * ESnet
Projects
 * Infrastructure
Projects
 * Staff
Projects
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Update
 New
hires:
 * Hing
Chow:

Project
Manager
(ANI)
 * Chris
Tracy:

Network
/
SoVware
Engineer
(ANI)
 * Andy
Lake:

SoVware
Engineer
(ANI)
 *

  17. Sugar loss and enzyme inhibition due to oligosaccharide accumulation during high solids-loading enzymatic hydrolysis

    Office of Scientific and Technical Information (OSTI)

    Xue et al. Biotechnol Biofuels (2015) 8:195 DOI 10.1186/S13068-015-0378-9 Biotechnology for Biofuels RESEARCH Open Access Sugar loss and enzyme inhibition due to oligosaccharide accumulation during high solids-loading enzymatic hydrolysis CrossMark Saisi Xue* 1* , Nirmal Uppugundla1*, Michael J. Bowman2, David Cavalier1,3, Leonardo Da Costa Sousa1, Bruce. E Dale1 and Venkatesh Balan1* Abstract Background: Accumulation of recalcitrant oligosaccharides during high-solids loading enzymatic

  18. Development of an Integrated Distribution Management System

    SciTech Connect (OSTI)

    Schatz, Joe E.

    2010-10-20

    This final report details the components, functionality, costs, schedule and benefits of developing an Integrated Distribution Management System (IDMS) for power distribution system operation. The Distribution Automation (DA) and Supervisory Control and Data Acquisition (SCADA) systems used by electric power companies to manage the distribution of electric power to retail energy consumers are vital components of the Nation’s critical infrastructure. Providing electricity is an essential public service and a disruption in that service, if not quickly restored, could threaten the public safety and the Nation’s economic security. Our Nation’s economic prosperity and quality of life have long depended on the essential services that utilities provide; therefore, it is necessary to ensure that electric utilities are able to conduct their operations safely and efficiently. A fully integrated technology of applications is needed to link various remote sensing, communications and control devices with other information tools that help guide Power Distribution Operations personnel. A fully implemented IDMS will provide this, a seamlessly integrated set of applications to raise electric system operating intelligence. IDMS will enhance DA and SCADA through integration of applications such as Geographic Information Systems, Outage Management Systems, Switching Management and Analysis, Operator Training Simulator, and other Advanced Applications, including unbalanced load flow and fault isolation/service restoration. These apps are capable of utilizing and obtaining information from appropriately installed DER, and by integrating disparate systems, the Distribution Operators will benefit from advanced capabilities when analyzing, controlling and operating the electric system.

  19. Development of fine-resolution analyses and expanded large-scale forcing properties. Part I: Methodology and evaluation

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Li, Zhijin; Vogelmann, Andrew M.; Feng, Sha; Liu, Yangang; Lin, Wuyin; Zhang, Minghua; Toto, Tami; Endo, Satoshi

    2015-01-20

    We produce fine-resolution, three-dimensional fields of meteorological and other variables for the U.S. Department of Energy’s Atmospheric Radiation Measurement (ARM) Southern Great Plains site. The Community Gridpoint Statistical Interpolation system is implemented in a multiscale data assimilation (MS-DA) framework that is used within the Weather Research and Forecasting model at a cloud-resolving resolution of 2 km. The MS-DA algorithm uses existing reanalysis products and constrains fine-scale atmospheric properties by assimilating high-resolution observations. A set of experiments show that the data assimilation analysis realistically reproduces the intensity, structure, and time evolution of clouds and precipitation associated with a mesoscale convective system.more » Evaluations also show that the large-scale forcing derived from the fine-resolution analysis has an overall accuracy comparable to the existing ARM operational product. For enhanced applications, the fine-resolution fields are used to characterize the contribution of subgrid variability to the large-scale forcing and to derive hydrometeor forcing, which are presented in companion papers.« less

  20. DAFNE Setup And Operation With the Crab-Waist Collision Scheme

    SciTech Connect (OSTI)

    Milardi, C.; Alesini, D.; Biagini, M.E.; Biscari, C.; Boni, R.; Boscolo, M.; Bossi, F.; Buonomo, B.; Clozza, A.; Delle Monache, G.; Demma, T.; Di Pasquale, E.; Di Pirro, G.; Drago, A.; Gallo, A.; Ghigo, A.; Guiducci, S.; Ligi, C.; Marcellini, F.; Mazzitelli, G.; Murtas, F.; /Frascati /Orsay, LAL /Novosibirsk, IYF /CERN /Rome III U. /Rome U. /KEK, Tsukuba /INFN, Pisa /Pisa U. /INFN, Cosenza /INFN, Rome /SLAC

    2011-10-21

    In the second half of 2007 a major upgrade has been implemented on the Frascati DA{Phi}NE collider in order to test the novel idea of Crab-Waist collisions. New vacuum chambers and permanent quadrupole magnets have been designed, built and installed to realize the new configuration. At the same time the performances of relevant hardware components, such as fast injection kickers and shielded bellows have been improved relying on new design concepts. The collider has been successfully commissioned in this new configuration. The paper describes several experimental results about linear and non-linear optics setup and optimization, damping of beam-beam instabilities and discusses the obtained luminosity performances. DA{Phi}NE [1] is the Frascati lepton collider working at the c m. energy of the {Phi} meson resonance (1020). It came in operation in 2001 and till summer 2007 provided luminosity, in sequence, to three different experiments which logged a total integrated luminosity of {approx} 4.4 fb{sup -1}. During these years the collider reached its best performances in terms of luminosity and background (L{sub peak} = 1.6 x 10{sup 32} cm{sup -2}s{sup -1} L{sub day} {approx} 10 pb{sup -1}) by means of several successive upgrades, relying on the experience gathered during the collider operations and implemented exploiting the shutdowns required for the experiment change over [2, 3, 4].

  1. Impact of Polymer Electrolyte Membrane Degradation Products on Oxygen Reduction Reaction Activity for Platinum Electrocatalysts

    SciTech Connect (OSTI)

    Christ, J. M.; Neyerlin, K. C.; Wang, H.; Richards, R.; Dinh, H. N.

    2014-10-30

    The impact of model membrane degradation compounds on the relevant electrochemical parameters for the oxygen reduction reaction (i.e. electrochemical surface area and catalytic activity), was studied for both polycrystalline Pt and carbon supported Pt electrocatalysts. Model compounds, representing previously published, experimentally determined polymer electrolyte membrane degradation products, were in the form of perfluorinated organic acids that contained combinations of carboxylic and/or sulfonic acid functionality. Perfluorinated carboxylic acids of carbon chain length C1 – C6 were found to have an impact on electrochemical surface area (ECA). The longest chain length acid also hindered the observed oxygen reduction reaction (ORR) performance, resulting in a 17% loss in kinetic current (determined at 0.9 V). Model compounds containing sulfonic acid functional groups alone did not show an effect on Pt ECA or ORR activity. Lastly, greater than a 44% loss in ORR activity at 0.9V was observed for diacid model compounds DA-Naf (perfluoro(2-methyl-3-oxa-5-sulfonic pentanoic) acid) and DA-3M (perfluoro(4-sulfonic butanoic) acid), which contained both sulfonic and carboxylic acid functionalities.

  2. Antenna unit and radio base station therewith

    DOE Patents [OSTI]

    Kuwahara, Mikio; Doi, Nobukazu; Suzuki, Toshiro; Ishida, Yuji; Inoue, Takashi; Niida, Sumaru

    2007-04-10

    Phase and amplitude deviations, which are generated, for example, by cables connecting an array antenna of a CDMA base station and the base station, are calibrated in the baseband. The base station comprises: an antenna apparatus 1; couplers 2; an RF unit 3 that converts a receive signal to a baseband signal, converts a transmit signal to a radio frequency, and performs power control; an A/D converter 4 for converting a receive signal to a digital signal; a receive beam form unit 6 that multiplies the receive signal by semi-fixed weight; a despreader 7 for this signal input; a time-space demodulator 8 for demodulating user data; a despreader 9 for probe signal; a space modulator 14 for user data; a spreader 13 for user signal; a channel combiner 12; a Tx calibrater 11 for controlling calibration of a signal; a D/A converter 10; a unit 16 for calculation of correlation matrix for generating a probe signal used for controlling an Rx calibration system and a TX calibration system; a spreader 17 for probe signal; a power control unit 18; a D/A converter 19; an RF unit 20 for probe signal; an A/D converter 21 for signal from the couplers 2; and a despreader 22.

  3. Impact of Polymer Electrolyte Membrane Degradation Products on Oxygen Reduction Reaction Activity for Platinum Electrocatalysts

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Christ, J. M.; Neyerlin, K. C.; Wang, H.; Richards, R.; Dinh, H. N.

    2014-10-30

    The impact of model membrane degradation compounds on the relevant electrochemical parameters for the oxygen reduction reaction (i.e. electrochemical surface area and catalytic activity), was studied for both polycrystalline Pt and carbon supported Pt electrocatalysts. Model compounds, representing previously published, experimentally determined polymer electrolyte membrane degradation products, were in the form of perfluorinated organic acids that contained combinations of carboxylic and/or sulfonic acid functionality. Perfluorinated carboxylic acids of carbon chain length C1 – C6 were found to have an impact on electrochemical surface area (ECA). The longest chain length acid also hindered the observed oxygen reduction reaction (ORR) performance, resultingmore » in a 17% loss in kinetic current (determined at 0.9 V). Model compounds containing sulfonic acid functional groups alone did not show an effect on Pt ECA or ORR activity. Lastly, greater than a 44% loss in ORR activity at 0.9V was observed for diacid model compounds DA-Naf (perfluoro(2-methyl-3-oxa-5-sulfonic pentanoic) acid) and DA-3M (perfluoro(4-sulfonic butanoic) acid), which contained both sulfonic and carboxylic acid functionalities.« less

  4. Measured daylighting potential of a static optical louver system under real sun and sky conditions

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Konis, Kyle; Lee, Eleanor S.

    2015-05-04

    Side-by-side comparisons were made over solstice-to-solstice changes in sun and sky conditions between an optical louver system (OLS) and a conventional Venetian blind set at a horizontal slat angle and located inboard of a south-facing, small-area, clerestory window in a full-scale office testbed. Daylight autonomy (DA), window luminance, and ceiling luminance uniformity were used to assess performance. The performance of both systems was found to have significant seasonal variation, where performance under clear sky conditions improved as maximum solar altitude angles transitioned from solstice to equinox. Although the OLS produced fewer hours per day of DA on average than themore » Venetian blind, the OLS never exceeded the designated 2000 cd/m2 threshold for window glare. In contrast, the Venetian blind was found to exceed the visual discomfort threshold over a large fraction of the day during equinox conditions. Notably, these peak periods of visual discomfort occurred during the best periods of daylighting performance. Luminance uniformity was analyzed using calibrated high dynamic range luminance images. Under clear sky conditions, the OLS was found to increase the luminance of the ceiling as well as produce a more uniform distribution. Furthermore, compared to conventional venetian blinds, the static optical sunlight redirecting system studied has the potential to significantly reduce the annual electrical lighting energy demand of a daylit space and improve the quality from the perspective of building occupants by consistently transmitting useful daylight while eliminating window glare.« less

  5. Characterization of the human oncogene SCL/TAL1 interrupting locus (Stil) mediated Sonic hedgehog (Shh) signaling transduction in proliferating mammalian dopaminergic neurons

    SciTech Connect (OSTI)

    Sun, Lei; Carr, Aprell L.; Li, Ping; Lee, Jessica; McGregor, Mary; Li, Lei

    2014-07-11

    Highlights: Stil is a human oncogene that is conserved in vertebrate species. Stil functions in the Shh pathway in mammalian cells. The expression of Stil is required for mammalian dopaminergic cell proliferation. - Abstract: The human oncogene SCL/TAL1 interrupting locus (Stil) is highly conserved in all vertebrate species. In humans, the expression of Stil is involved in cancer cell survival, apoptosis and proliferation. In this research, we investigated the roles of Stil expression in cell proliferation of mammalian dopaminergic (DA) PC12 cells. Stil functions through the Sonic hedgehog (Shh) signal transduction pathway. Co-immunoprecipitation tests revealed that STIL interacts with Shh downstream components, which include SUFU and GLI1. By examining the expression of Stil, Gli1, CyclinD2 (cell-cycle marker) and PCNA (proliferating cell nuclear antigen), we found that up-regulation of Stil expression (transfection with overexpression plasmids) increased Shh signaling transduction and PC12 cell proliferation, whereas down-regulation of Stil expression (by shRNA) inhibited Shh signaling transduction, and thereby decreased PC12 cell proliferation. Transient transfection of PC12 cells with Stil knockdown or overexpression plasmids did not affect PC12 cell neural differentiation, further indicating the specific roles of Stil in cell proliferation. The results from this research suggest that Stil may serve as a bio-marker for neurological diseases involved in DA neurons, such as Parkinsons disease.

  6. Metabolomic changes in follicular fluid induced by soy isoflavones administered to rats from weaning until sexual maturity

    SciTech Connect (OSTI)

    Wang, Wenxiang; Zhang, Wenchang; Liu, Jin; Sun, Yan; Li, Yuchen; Li, Hong; Xiao, Shihua; Shen, Xiaohua

    2013-06-15

    Female Wistar rats at 21 days of age were treated with one of three concentrations of soy isoflavones (SIF) (50, 100 or 200 mg/kg body weight, orally, once per day) from weaning until sexual maturity (3 months) in order to evaluate the influence of SIF on ovarian follicle development. After treatment, the serum sex hormone levels and enumeration of ovarian follicles of the ovary were measured. The metabolic profile of follicular fluid was determined using HPLC-MS. Principal component analysis (PCA) and partial least-squares-discriminant analysis (PLS-DA) was used to identify differences in metabolites and reveal useful toxic biomarkers. The results indicated that modest doses of SIF affect ovarian follicle development, as demonstrated by decreased serum estradiol levels and increases in both ovarian follicle atresia and corpora lutea number in the ovary. SIF treatment-related metabolic alterations in follicular fluid were also found in the PCA and PLS-DA models. The 24 most significantly altered metabolites were identified, including primary sex hormones, amino acids, fatty acids and metabolites involved in energy metabolism. These findings may indicate that soy isoflavones affect ovarian follicle development by inducing metabolomic variations in the follicular fluid. - Highlights: ► Modest doses of soy isoflavones (SIF) do affect ovarian follicle development. ► SIF treatment-related metabolic alterations in follicular fluid were found. ► The 24 most significantly altered metabolites were identified.

  7. An Optimized Autoregressive Forecast Error Generator for Wind and Load Uncertainty Study

    SciTech Connect (OSTI)

    De Mello, Phillip; Lu, Ning; Makarov, Yuri V.

    2011-01-17

    This paper presents a first-order autoregressive algorithm to generate real-time (RT), hour-ahead (HA), and day-ahead (DA) wind and load forecast errors. The methodology aims at producing random wind and load forecast time series reflecting the autocorrelation and cross-correlation of historical forecast data sets. Five statistical characteristics are considered: the means, standard deviations, autocorrelations, and cross-correlations. A stochastic optimization routine is developed to minimize the differences between the statistical characteristics of the generated time series and the targeted ones. An optimal set of parameters are obtained and used to produce the RT, HA, and DA forecasts in due order of succession. This method, although implemented as the first-order regressive random forecast error generator, can be extended to higher-order. Results show that the methodology produces random series with desired statistics derived from real data sets provided by the California Independent System Operator (CAISO). The wind and load forecast error generator is currently used in wind integration studies to generate wind and load inputs for stochastic planning processes. Our future studies will focus on reflecting the diurnal and seasonal differences of the wind and load statistics and implementing them in the random forecast generator.

  8. ADA/SCADA RTU protocol based on the 3-layer UCA model

    SciTech Connect (OSTI)

    Adamo, V.P.

    1995-12-31

    This paper describes an implementation of a DA/SCADA RTU communication protocol based on the 3-layer reference model for wide area networks specified in the Utility Communications Architecture (UCA) VL1.0. This protocol is based on the following international standards: EIA-232-D, High-level Data Link Control (HDLC) [ISO/IEC 3309], and Manufacturing Message Specification (MMS) [ISO/IEC 95061]. A description of the HDLC frame structure used in this implementation is provided. This includes a description of the extended transparency option for Start/Stop transmission, commonly referred to as {open_quotes}asynchronous HDLC{close_quotes}. This option allows for the transmission of HDLC frames using inexpensive asynchronous communication hardware. The data link topology described in this paper is an unbalanced, point-to-multipoint topology consisting of one primary, or, master station, and multiple secondary, or remote, stations. The data link operates in the Normal Response Mode (NRM). In this mode a secondary station may initiate transmissions only as a result of receiving explicit permission to do so from the primary station. The application layer protocol described in this paper is an implementation of the Manufacturing Message Specification (MMS). The MMS device model, or Virtual Manufacturing Device (VMD), for a DA/SCADA Remote Terminal Unit is provided. The current VMD model provides a view of common RTU data types, plus, A/C Input (ACI) data including phasor magnitude and mean readings, harmonics, and overcurrent alarm information.

  9. Measured daylighting potential of a static optical louver system under real sun and sky conditions

    SciTech Connect (OSTI)

    Konis, Kyle; Lee, Eleanor S.

    2015-05-04

    Side-by-side comparisons were made over solstice-to-solstice changes in sun and sky conditions between an optical louver system (OLS) and a conventional Venetian blind set at a horizontal slat angle and located inboard of a south-facing, small-area, clerestory window in a full-scale office testbed. Daylight autonomy (DA), window luminance, and ceiling luminance uniformity were used to assess performance. The performance of both systems was found to have significant seasonal variation, where performance under clear sky conditions improved as maximum solar altitude angles transitioned from solstice to equinox. Although the OLS produced fewer hours per day of DA on average than the Venetian blind, the OLS never exceeded the designated 2000 cd/m2 threshold for window glare. In contrast, the Venetian blind was found to exceed the visual discomfort threshold over a large fraction of the day during equinox conditions. Notably, these peak periods of visual discomfort occurred during the best periods of daylighting performance. Luminance uniformity was analyzed using calibrated high dynamic range luminance images. Under clear sky conditions, the OLS was found to increase the luminance of the ceiling as well as produce a more uniform distribution. Furthermore, compared to conventional venetian blinds, the static optical sunlight redirecting system studied has the potential to significantly reduce the annual electrical lighting energy demand of a daylit space and improve the quality from the perspective of building occupants by consistently transmitting useful daylight while eliminating window glare.

  10. Field Tolerances for the Triplet Quadrupoles of the LHC High Luminosity Lattice

    SciTech Connect (OSTI)

    Nosochkov, Yuri; Cai, Y.; Jiao, Y.; Wang, M-H.; /SLAC; Fartoukh, S.; Giovannozzi, M.; Maria, R.de; McIntosh, E.; ,

    2012-06-25

    It has been proposed to implement the so-called Achromatic Telescopic Squeezing (ATS) scheme in the LHC high luminosity (HL) lattice to reduce beta functions at the Interaction Points (IP) up to a factor of 8. As a result, the nominal 4.5 km peak beta functions reached in the Inner Triplets (IT) at collision will be increased by the same factor. This, therefore, justifies the installation of new, larger aperture, superconducting IT quadrupoles. The higher beta functions will enhance the effects of the triplet quadrupole field errors leading to smaller beam dynamic aperture (DA). To maintain the acceptable DA, the effects of the triplet field errors must be re-evaluated, thus specifying new tolerances. Such a study has been performed for the so-called '4444' collision option of the HL-LHC layout version SLHCV3.01, where the IP beta functions are reduced by a factor of 4 in both planes with respect to a pre-squeezed value of 60 cm at two collision points. The dynamic aperture calculations were performed using SixTrack. The impact on the triplet field quality is presented.

  11. Safeguards Verification Measurements using Laser Ablation, Absorbance Ratio Spectrometry in Gaseous Centrifuge Enrichment Plants

    SciTech Connect (OSTI)

    Anheier, Norman C.; Cannon, Bret D.; Kulkarni, Gourihar R.; Munley, John T.; Nelson, Danny A.; Qiao, Hong; Phillips, Jon R.

    2012-07-17

    Laser Ablation Absorbance Ratio Spectrometry (LAARS) is a new verification measurement technology under development at the US Department of Energy (DOE) Pacific Northwest National Laboratory (PNNL). LAARS uses three lasers to ablate and then measure the relative isotopic abundance of uranium compounds. An ablation laser is tightly focused on uranium-bearing solids, producing a small atomic uranium vapor plume. Two collinear wavelength-tuned spectrometry lasers transit through the plume and the absorbance of U-235 and U-238 isotopes are measured to determine U-235 enrichment. The measurement is independent of chemical form and degree of dilution with nuisance dust and other materials. LAARS has high relative precision and detection limits approaching the femtogram range for U-235. The sample is scanned and assayed point-by-point at rates reaching 1 million measurements/hour, enabling LAARS to detect and analyze uranium in trace samples. The spectrometer is assembled using primarily commercially available components and features a compact design and automated analysis.Two specific gaseous centrifuge enrichment plant (GCEP) applications of the spectrometer are currently under development: 1) LAARS-Environmental Sampling (ES), which collects and analyzes aerosol particles for GCEP misuse detection and 2) LAARS-Destructive Assay (DA), which enables onsite enrichment DA sample collection and analysis for protracted diversion detection. The two applications propose game-changing technological advances in GCEP safeguards verification.

  12. Human interleukin 2 receptor. beta. -chain gene: Chromosomal localization and identification of 5 prime regulatory sequences

    SciTech Connect (OSTI)

    Gnarra, J.R.; Otani, Hiroki; Wang, M.G.; McBride, O.W.; Sharon, M.; Leonard, W.J. )

    1990-05-01

    Interleukin 2 (IL-2) binds to and stimulates activated T cells through high-affinity IL-2 receptors (IL-2Rs). Such receptors represent a complex consisting of at least two proteins, the 55-kDa IL-2R{alpha} chain and the 70-kDa IL-2R{beta} chain. The low-affinity, IL-2R{alpha} chain cannot by itself transduce a mitogenic signal, whereas IL-2 stimulates resting lymphocytes through the intermediate-affinity, IL-2R{beta} receptor. The authors report here identification of the genomic locus for IL-2R{beta}. The exons are contained on four EcoRI fragments of 1.1, 9.2, 7.2, and 13.7 kilobases. The 1.1-kilobase EcoRI fragment lies at the 5{prime}-most end of the genomic locus and contains promoter sequences. The promoter contains no TATA box-like elements but does contain the d(GT){sub n} class of middle repetitive elements, which may play an interesting regulatory role. The IL-2R{beta} gene is localized to chromosome 22q11.2-q12, a region that is the locus for several lymphoid neoplasias.

  13. Amorphous iron–chromium oxide nanoparticles with long-term stability

    SciTech Connect (OSTI)

    Iacob, Mihail; Cazacu, Maria; Turta, Constantin; Doroftei, Florica; Botko, Martin; Čižmár, Erik; Zeleňáková, Adriana; Feher, Alexander

    2015-05-15

    Highlights: • Fe–Cr oxide nanoparticles with pre-established metals ratio were obtained. • The amorphous state and its long-term stability were highlighted by X-ray diffraction. • The average diameter of dried nanoparticles was 3.5 nm, as was estimated by TEM, AFM. • In hexane dispersion, nanoparticles with diameter in the range 2.33–4.85 nm were found. • Superparamagnetic state of NPs co-exists with diamagnetism of the organic layer. - Abstract: Iron–chromium nanoparticles (NPs) were obtained through the thermal decomposition of μ{sub 3}-oxo heterotrinuclear (FeCr{sub 2}O) acetate in the presence of sunflower oil and dodecylamine (DA) as surfactants. The average diameter of the NPs was 3.5 nm, as estimated on the basis of transmission electron microscopy and atomic force microscopy images. Both techniques revealed the formation of roughly approximated spheres with some irregularities and agglomerations in larger spherical assemblies of 50–100 nm. In hexane, NPs with diameters in the 2.33–4.85 nm range are individually dispersed, as emphasized by dynamic light scattering measurements. The amorphous nature of the product was emphasized by X-ray powder diffraction. The study of the magnetic properties shows the presence of superparamagnetic state of iron–chromium oxide NPs and the diamagnetic contribution from DA layer forming a shell of NPs.

  14. Simultaneous cloning and expression of two cellulase genes from Bacillus subtilis newly isolated from Golden Takin (Budorcas taxicolor Bedfordi)

    SciTech Connect (OSTI)

    Li, Wang; College of Animal Science, Henan Science and Technology University, Luoyang 471003 ; Huan, Xiajuan; Zhou, Ying; Ma, Qingyi; Chen, Yulin

    2009-06-12

    A bacterial strain with high cellulase activity was isolated of feces sample of Golden Takin (Budorcas taxicolor Bedfordi). The bacterium was classified and designated Bacillus subtilis LN by morphological and 16SrDNA gene sequence analysis. Two putative cellulase genes, CelL15 and CelL73, were simultaneously cloned from the isolated strain by PCR. The putative gene CelL15 consisted of an open reading frame (ORF) of 1470 nucleotides and encoded a protein of 490 amino acids with a molecular weight of 54 kDa. The CelL73 gene consisted of an open reading frame (ORF) of 741 nucleotides and encoded a protein of 247 amino acids with a molecular weight of 27 kDa. Both genes were purified and cloned into pET-28a for expression in Escherichia coli BL21 (DE3). The ability of E. coli to degrade cellulose was enhanced when the two recombinants were cultured together.

  15. Improving Site-Specific Radiological Performance Assessments - 13431

    SciTech Connect (OSTI)

    Tauxe, John; Black, Paul; Catlett, Kate; Lee, Robert; Perona, Ralph; Stockton, Tom; Sully, Mike

    2013-07-01

    An improved approach is presented for conducting complete and defensible radiological site-specific performance assessments (PAs) to support radioactive waste disposal decisions. The basic tenets of PA were initiated some thirty years ago, focusing on geologic disposals and evaluating compliance with regulations. Some of these regulations were inherently probabilistic (i.e., addressing uncertainty in a quantitative fashion), such as the containment requirements of the U.S. Environmental Protection Agency's (EPA's) 40 CFR 191, Environmental Radiation Protection Standards for Management and Disposal of Spent Nuclear Fuel, High-Level and Transuranic Radioactive Wastes, Chap. 191.13 [1]. Methods of analysis were developed to meet those requirements, but at their core early PAs used 'conservative' parameter values and modeling approaches. This limited the utility of such PAs to compliance evaluation, and did little to inform decisions about optimizing disposal, closure and long-term monitoring and maintenance, or, in general, maintaining doses 'as low as reasonably achievable' (ALARA). This basic approach to PA development in the United States was employed essentially unchanged through the end of the 20. century, principally by the U.S. Department of Energy (DOE). Performance assessments developed in support of private radioactive waste disposal operations, regulated by the U.S. Nuclear Regulatory Commission (NRC) and its agreement states, were typically not as sophisticated. Discussion of new approaches to PA is timely, since at the time of this writing, the DOE is in the midst of revising its Order 435.1, Radioactive Waste Management [2], and the NRC is revising 10 CFR 61, Licensing Requirements for Land Disposal of Radioactive Waste [3]. Over the previous decade, theoretical developments and improved computational technology have provided the foundation for integrating decision analysis (DA) concepts and objective-focused thinking, plus a Bayesian approach to probabilistic modeling and risk analysis, to guide improvements in PA. This decision-making approach, [4, 5, 6] provides a transparent formal framework for using a value- or objective-focused approach to decision-making. DA, as an analytical means to implement structured decision making, provides a context for both understanding how uncertainty affects decisions and for targeting uncertainty reduction. The proposed DA approach improves defensibility and transparency of decision-making. The DA approach is fully consistent with the need to perform realistic modeling (rather than conservative modeling), including evaluation of site-specific factors. Instead of using generic stylized scenarios for radionuclide fate and transport and for human exposures to radionuclides, site-specific scenarios better represent the advantages and disadvantages of alternative disposal sites or engineered designs, thus clarifying their differences as well as providing a sound basis for evaluation of site performance. The full DA approach to PA is described, from explicitly incorporating societal values through stakeholder involvement to model building. Model building involves scoping by considering features, events, processes, and exposure scenarios (FEPSs), development of a conceptual site model (CSM), translation into numerical models and subsequent computation, and model evaluation. These are implemented in a cycle of uncertainty analysis, sensitivity analysis and value of information analysis so that uncertainty can be reduced until sufficient confidence is gained in the decisions to be made. This includes the traditional focus on hydrogeological processes, but also places emphasis on other FEPSs such as biotically-induced transport and human exposure phenomena. The significance of human exposure scenarios is emphasized by modifying the traditional acronym 'FEPs' to include them, hence 'FEPSs'. The radioactive waste community is also recognizing that disposal sites are to be considered a national (or even global) resource. As such, there is a pressing need to optimize their utility withi

  16. Integrating Solar PV in Utility System Operations

    SciTech Connect (OSTI)

    Mills, A.; Botterud, A.; Wu, J.; Zhou, Z.; Hodge, B-M.; Heany, M.

    2013-10-31

    This study develops a systematic framework for estimating the increase in operating costs due to uncertainty and variability in renewable resources, uses the framework to quantify the integration costs associated with sub-hourly solar power variability and uncertainty, and shows how changes in system operations may affect these costs. Toward this end, we present a statistical method for estimating the required balancing reserves to maintain system reliability along with a model for commitment and dispatch of the portfolio of thermal and renewable resources at different stages of system operations. We estimate the costs of sub-hourly solar variability, short-term forecast errors, and day-ahead (DA) forecast errors as the difference in production costs between a case with “realistic” PV (i.e., subhourly solar variability and uncertainty are fully included in the modeling) and a case with “well behaved” PV (i.e., PV is assumed to have no sub-hourly variability and can be perfectly forecasted). In addition, we highlight current practices that allow utilities to compensate for the issues encountered at the sub-hourly time frame with increased levels of PV penetration. In this analysis we use the analytical framework to simulate utility operations with increasing deployment of PV in a case study of Arizona Public Service Company (APS), a utility in the southwestern United States. In our analysis, we focus on three processes that are important in understanding the management of PV variability and uncertainty in power system operations. First, we represent the decisions made the day before the operating day through a DA commitment model that relies on imperfect DA forecasts of load and wind as well as PV generation. Second, we represent the decisions made by schedulers in the operating day through hour-ahead (HA) scheduling. Peaking units can be committed or decommitted in the HA schedules and online units can be redispatched using forecasts that are improved relative to DA forecasts, but still imperfect. Finally, we represent decisions within the operating hour by schedulers and transmission system operators as real-time (RT) balancing. We simulate the DA and HA scheduling processes with a detailed unit-commitment (UC) and economic dispatch (ED) optimization model. This model creates a least-cost dispatch and commitment plan for the conventional generating units using forecasts and reserve requirements as inputs. We consider only the generation units and load of the utility in this analysis; we do not consider opportunities to trade power with neighboring utilities. We also do not consider provision of reserves from renewables or from demand-side options. We estimate dynamic reserve requirements in order to meet reliability requirements in the RT operations, considering the uncertainty and variability in load, solar PV, and wind resources. Balancing reserve requirements are based on the 2.5th and 97.5th percentile of 1-min deviations from the HA schedule in a previous year. We then simulate RT deployment of balancing reserves using a separate minute-by-minute simulation of deviations from the HA schedules in the operating year. In the simulations we assume that balancing reserves can be fully deployed in 10 min. The minute-by-minute deviations account for HA forecasting errors and the actual variability of the load, wind, and solar generation. Using these minute-by-minute deviations and deployment of balancing reserves, we evaluate the impact of PV on system reliability through the calculation of the standard reliability metric called Control Performance Standard 2 (CPS2). Broadly speaking, the CPS2 score measures the percentage of 10-min periods in which a balancing area is able to balance supply and demand within a specific threshold. Compliance with the North American Electric Reliability Corporation (NERC) reliability standards requires that the CPS2 score must exceed 90% (i.e., the balancing area must maintain adequate balance for 90% of the 10-min periods). The combination of representing DA forecast errors in the DA commitments, using 1-min PV data to simulate RT balancing, and estimates of reliability performance through the CPS2 metric, all factors that are important to operating systems with increasing amounts of PV, makes this study unique in its scope.

  17. 1.1-silicon.pptx

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Tutorial 1 : B asic G W c alcula1ons o n s ilicon David A . S trubbe Department o f M aterials S cience a nd E ngineering, Massachuse8s I ns:tute o f T echnology BerkeleyGW t utorial 22 November 2013 Workflow f or t he t utorial SCF WFN WFNq WFN_inner RHO vxc.dat WFN_fi epsilon sigma inteqp kgrid.x wfn_rho_vxc_info.x Epsilon: s creening a s a f unc1on o f | q| 0 2 4 6 8 10 12 14 16 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 |q| ε 0,0 (q) 1/ε -1 0,0 (q) Sigma: Q P c orrec1ons a s a f unc1on o f L DA

  18. NSTX-U Control System Upgrades

    Office of Scientific and Technical Information (OSTI)

    5 PPPL- 5045 NSTX-U Control System Upgrades K.G. Erickson, D.A. Gates, S.P. Gerhardt, J.E. Lawson, R. Mozulay, P. Sichta, G.J. Tchilinguirian May 2013 5 ) PPPL P R IN C E T O N P L A S M A PHYSIC S L A B O R A T O R Y Prepared for the U.S. Department of Energy under Contract DE-AC02-09CH11466. P r i n c e t o n P la s m a P h y s ic s L a b o r a to r y R e p o r t D is c la i m e r s F u ll L egal D iscla im er This report was p repared as an account of work sponsored by an agency of the U

  19. Low Head, Vortex Induced Vibrations River Energy Converter

    SciTech Connect (OSTI)

    Bernitsas, Michael B.; Dritz, Tad

    2006-06-30

    Vortex Induced Vibrations Aquatic Clean Energy (VIVACE) is a novel, demonstrated approach to extracting energy from water currents. This invention is based on a phenomenon called Vortex Induced Vibrations (VIV), which was first observed by Leonardo da Vinci in 1504AD. He called it ‘Aeolian Tones.’ For decades, engineers have attempted to prevent this type of vibration from damaging structures, such as offshore platforms, nuclear fuel rods, cables, buildings, and bridges. The underlying concept of the VIVACE Converter is the following: Strengthen rather than spoil vortex shedding; enhance rather than suppress VIV; harness rather than mitigate VIV energy. By maximizing and utilizing this unique phenomenon, VIVACE takes this “problem” and successfully transforms it into a valuable resource for mankind.

  20. OT SPECIFIED I OTHER AMENDMENT OF SOLICITATI ON/MODIFICATION OF CONTRACT

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    OT SPECIFIED I OTHER AMENDMENT OF SOLICITATI ON/MODIFICATION OF CONTRACT 2 AM EN DMENT/MODIFIC ATION NO 1 B 6 ISSUED BY CODE Oak UrJge u . s . De arcment of Energ y P . O. Box 2001 Oak Ridge TN 37831 3 EFFccnv E DA E Sep Bl c..c.k _6C 00518 8 NAME AND ADDRESS OF CONTRACTOR INo ~/e.' CO Ull/y. Sial. and ZIP Cod.) OAK RIDGE A SOCIATED Ul IVERSITIES , P . O. BOX 117 OAK R-DGE Ttl 37830-6218 N . CODE 0411522 24 FAC ILITY CODE 1 CONTRACT 10 CODE 4 R OUISITIONIPU RCHASr. REO NO IuS lL 7 ADMIN ISTER ED

  1. Annual Site Environmental Report Paducah Site

    Energy Savers [EERE]

    Site Environmental Report Paducah Site 2011 PAD-REG-1012 BACK TABLE OF CONTENTS FORWARD Fractions and Multiples of Units Multiple Decimal Equivalent Prefix Symbol Engineering Format 10 6 1,000,000 mega- M E+06 10 3 1,000 kilo- k E+03 10 2 100 hecto- h E+02 10 10 deka- da E+01 10 -1 0.1 deci- d E-01 10 -2 0.01 centi- c E-02 10 -3 0.001 milli- m E-03 10 -6 0.000001 micro- μ E-06 10 -9 0.000000001 nano- n E-09 10 -12 0.000000000001 pico- P E-12 10 -15 0.000000000000001 femto- F E-15 10 -18

  2. Retinoblastoma protein co-purifies with proteasomal insulin-degrading enzyme: Implications for cell proliferation control

    SciTech Connect (OSTI)

    Radulescu, Razvan T., E-mail: ratura@gmx.net [Molecular Concepts Research (MCR), Muenster (Germany); Duckworth, William C. [Department of Medicine, Phoenix VA Health Care System, Phoenix, AZ (United States)] [Department of Medicine, Phoenix VA Health Care System, Phoenix, AZ (United States); Levy, Jennifer L. [Research Service, Phoenix VA Health Care System, Phoenix, AZ (United States)] [Research Service, Phoenix VA Health Care System, Phoenix, AZ (United States); Fawcett, Janet, E-mail: janet.fawcett@va.gov [Research Service, Phoenix VA Health Care System, Phoenix, AZ (United States)] [Research Service, Phoenix VA Health Care System, Phoenix, AZ (United States)

    2010-04-30

    Previous investigations on proteasomal preparations containing insulin-degrading enzyme (IDE; EC 3.4.24.56) have invariably yielded a co-purifying protein with a molecular weight of about 110 kDa. We have now found both in MCF-7 breast cancer and HepG2 hepatoma cells that this associated molecule is the retinoblastoma tumor suppressor protein (RB). Interestingly, the amount of RB in this protein complex seemed to be lower in HepG2 vs. MCF-7 cells, indicating a higher (cytoplasmic) protein turnover in the former vs. the latter cells. Moreover, immunofluorescence showed increased nuclear localization of RB in HepG2 vs. MCF-7 cells. Beyond these subtle differences between these distinct tumor cell types, our present study more generally suggests an interplay between RB and IDE within the proteasome that may have important growth-regulatory consequences.

  3. Longitudinal Beam Stability in the SUPER B-FACTORY

    SciTech Connect (OSTI)

    Novokhatski, A.; Zobov, M.; /Frascati

    2009-07-06

    We give an overview of wake fields and impedances in a proposed Super B project, which is based on extremely low emittance beams colliding at a large angle with a crab waist transformation. Understanding the effects that wake fields have on the beam is critical for a successful machine operation. We use our combined experience from the operation of the SLAC B-factory and DA{Phi}NE {Phi}-factory to eliminate strong HOM sources and minimize the chamber impedance in the Super B design. Based on a detailed study of the wake fields in this design we have developed a quasi-Green's function for the entire ring that is used to study bunch lengthening and beam stability. In particular, we check the stability threshold using numerical solutions of the Fokker-Plank equation. We also make a comparison of numerical simulations with the bunch lengthening data in the B- factory.

  4. Touschek Background and Lifetime Studies for the SuperB Factory

    SciTech Connect (OSTI)

    Boscolo, M.; Biagini, M.; Raimondi, P.; Sullivan, M.; Paoloni, E.; /INFN, Pisa

    2010-08-26

    The novel crab waist collision scheme under test at the DA{Phi}NE Frascati {Phi}-factory finds its natural application to the SuperB project, the asymmetric e{sup +}e{sup -} flavour factory at very high luminosity with relatively low beam currents and reduced backgrounds. The SuperB accelerator design requires a careful choice of beam parameters to reach a good trade-off between different effects. We present here simulation results for the Touschek backgrounds and lifetime obtained for both the low and high energy rings for different machine designs. A first set of horizontal collimators has been studied to stop Touschek particles. A study of the distributions of the Touschek particle losses at the interaction region into the detectors for further investigations is underway.

  5. Itaipu: never underestimate the Latins. [Paraguay/Brazil binational project

    SciTech Connect (OSTI)

    Not Available

    1983-04-06

    The Itaipu hydroelectric project, a joint effort of Brazil and Paraguay (with a cost of US $16 to 18 billion), will be finished in December 1989. The project is situated on the Parana River, 14 km beyond the Puente de da Amistad (Friendship Bridge), which connects the city Presidente Stroessner, in Paraguay, with Foz do Iguacu, in Brazil. It is considered today not only the biggest hydroelectric plant in the world, but also a great socio-economic boom in the making. Itaipu will add a total of 12.6-million kilowatt-hours (kWh) of hydroelectricity to the region, an equivalent of 600,000 barrels of oil daily (b/d). This issue of Energy Detente reviews the progress of Itaipu. Also appearing in this issue is the fuel price/tax series and the principal industrial fuel prices for April 1983 for countries of the Eastern Hemisphere.

  6. DISCLAIMER

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Lawrence Livermore National Laboratory U.S. Department of Energy Approved for public release; further dissemination unlimited UCRL-ID-151619 Results from the First 249 Cf+ 48 Ca Experiment Y.T. Oganessian, V.K. Utyonkov, Y.V. Lobanov, F.S. Abdullin, A.N. Polyakov, I.V. Shirokovsky, Y.S. Tsyganov, A.N. Mezentsev, S. Iliev, V.G. Subbotin, A.M. Sukhov, O.V. Ivanov, A.A. Voinov, K. Subotic, V.I. Zagrebaev, M.G. Itkis, K.J. Moody, J.F. Wild, M.A. Stoyer, N.J. Stoyer, C.A. Laue, D.A. Shaughnessy, J.B.

  7. DOE/NV--471 UC-700 U.S. Department of Energy Nevada Operations Office

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    471 UC-700 U.S. Department of Energy Nevada Operations Office on on on on : ed : 2 Approved for public release; further distribution is authorized. E nv i r onm ent a l R es t or a t i D i v i s i N ev ada E nv i r onm ent al R es t or at i P r oj ect C or r ect i v e A ct i on D eci s i D ocu m ent , S econd Ga s S t a t i on, Tonopa h Tes t R a nge, N ev a da ( C or r ect i v e A ct i on U ni t N o. 403) C ont r ol l ed C opy N o.U ncont r ol l R ev i s i on N o. N ov em ber 1997 This report

  8. Purification of 1-aminocyclopropane-1-carboxylate synthase from apple fruits using s-adenosyl (3,4 sup 14 C)-methionine (SAM) as a probe

    SciTech Connect (OSTI)

    Yip, Wingkip; Dong, Jianguo,; Yang, Shang Fa )

    1989-04-01

    Tomato ACC synthase is inactivated by its substrate SAM, with the moiety of aminobutyrate being covalently linked to ACC synthase during the catalytic reactions. A partial purified ACC synthase (the catalytic activity 100 {mu}mol/h{center dot}mg protein) from pellets of apple extract was incubated with (3,4{sup 14}C) SAM. Only one radioactive peak was revealed in a C-4 reverse phase HPLC and one radioactive band on SDS-PAGE with an M.W. of 48 kDa. Apple ACC synthase in native form is resistant to V8, {alpha}-chromtrypsin and carboxylpeptidase A digestion, but effectively inactivated by trypsin and ficin, as demonstrated by both the activity assay and SAM labeling. The radioactive protein cut from the SDS-PAGE was injected to three mice, two of the mice showed responses to the protein in western blot analysis. The antibodies from mice is currently under characterization.

  9. IL

    Office of Legacy Management (LM)

    :; / 2. ' b-:-"y .",...4 * .-.a 2 IL !< :. 34 --' -, ' ' < I ,-. g Tvo"l r . . .-i- :- " .1-. . . . . NC0 /L ' J,, ' ;.' , -_I( + ? CENTRAL FILES c -&' { ' c;$y ;;j*' E ,J): ' i' Z, 1; p -^ r-raL-r.nuzT".Fn., , ,..-y - -' -ie .". iJ.&:~e!ct.;;' sf ' ;;i_is ,trip ' JG,' go f-Jj;~ey~ 2123 -:s<j .-&;.z ;y1y rrc&ed recr!p!~-,da.a .b ,&.j.,& .:*3;.. F-Y ' __ ,,,.x+; PC l;ealti: :.2,.i CCI ;et y. tirlr.&g t;1c oy-er at ion c.f sei;a~xl

  10. Method for screening inhibitors of the toxicity of Bacillus anthracis

    DOE Patents [OSTI]

    Cirino, Nick M.; Jackson, Paul J.; Lehnert, Bruce E.

    2001-01-01

    The protective antigen (PA) of Bacillus anthracis is integral to the mechanism of anthrax poisoning. The cloning, expression and purification of a 32 kDa B. anthracis PA fragment (PA32) is described. This fragment has also been expressed as a fusion construct to stabilized green fluorescent protein (EGFP-PA32). Both proteins were capable of binding to specific cell surface receptors as determined by fluorescent microscopy and a flow cytometric assay. To confirm binding specificity in the flow cytometric assay, non-fluorescent PA83 or PA32 was used to competitively inhibit fluorescent EGFP-PA32 binding to cell receptors. This assay can be employed as a rapid screen for compounds which disrupts binding of PA to cells. Additionally, the high intracellular expression levels and ease of purification make this recombinant protein an attractive vaccine candidate or therapeutic treatment for anthrax poisoning.

  11. Kalman filter data assimilation: Targeting observations and parameter estimation

    SciTech Connect (OSTI)

    Bellsky, Thomas Kostelich, Eric J.; Mahalov, Alex

    2014-06-15

    This paper studies the effect of targeted observations on state and parameter estimates determined with Kalman filter data assimilation (DA) techniques. We first provide an analytical result demonstrating that targeting observations within the Kalman filter for a linear model can significantly reduce state estimation error as opposed to fixed or randomly located observations. We next conduct observing system simulation experiments for a chaotic model of meteorological interest, where we demonstrate that the local ensemble transform Kalman filter (LETKF) with targeted observations based on largest ensemble variance is skillful in providing more accurate state estimates than the LETKF with randomly located observations. Additionally, we find that a hybrid ensemble Kalman filter parameter estimation method accurately updates model parameters within the targeted observation context to further improve state estimation.

  12. Expression, purification, crystallization, data collection and preliminary biochemical characterization of methicillin-resistant Staphylococcus aureus Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5′-phosphate-dependent aminotransferase

    SciTech Connect (OSTI)

    Seetharamappa, Jaldappagari; Oke, Muse; Liu, Huanting; McMahon, Stephen A.; Johnson, Kenneth A.; Carter, Lester; Dorward, Mark; Zawadzki, Michal; Overton, Ian M.; Niekirk, C. A. Johannes van; Graham, Shirley; Botting, Catherine H.; Taylor, Garry L.; White, Malcolm F.; Barton, Geoffrey J.; Coote, Peter J.; Naismith, James H.

    2007-05-01

    As part of work on S. aureus, the crystallization of Sar2028, a protein that is upregulated in MRSA, is reported. Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5′-phosphate-dependent aminotransferase with a molecular weight of 48 168 Da, was overexpressed in methicillin-resistant Staphylococcus aureus compared with a methicillin-sensitive strain. The protein was expressed in Escherichia coli, purified and crystallized. The protein crystallized in a primitive orthorhombic Laue group with unit-cell parameters a = 83.6, b = 91.3, c = 106.0 Å, α = β = γ = 90°. Analysis of the systematic absences along the three principal axes indicated the space group to be P2{sub 1}2{sub 1}2{sub 1}. A complete data set was collected to 2.5 Å resolution.

  13. The Sao Paulo Microtron: Equipment and Planned Experiments

    SciTech Connect (OSTI)

    Martins, M. N.; Maidana, N. L.; Vanin, V. R.

    2007-10-26

    The Linear Accelerator Laboratory (LAL) of the Instituto de Fisica da Universidade de Sao Paulo (IFUSP) is building a two-stage racetrack microtron, which will generate continuous wave electron beams with energies up to 38 MeV. This paper describes the characteristics of the accelerator, and reports on the experimental equipment that will be available in order to pursue the photonuclear physics research program. Operation will begin with the first stage (5 MeV), and concentrate on NRF (Nuclear Resonance Fluorescence) measurements and radiation physics studies. Planned experiments for the second stage explore the cw character of the beam on coincidence experiments. A photon tagger has been already tested with radioactive sources and is ready to be installed. Gamma and neutron detector arrays are being developed for the detailed study of photoneutron reactions. Plans include the study of NRF and pygmy resonances, near the neutron binding energy.

  14. 4He Cross Section

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    α, X) (Current as of 05/14/2012) NSR Reaction Eα (MeV) Cross Section File X4 Dataset Date Added 2013DA10 4He(α, γ): deduced σ 19 - 29 X4 01/27/2016 1978HI04 4He(α, α): elastic scattering excitation function 32.6 - 35.4 θ = 30.5°, θ = 53.7°, θ = 54.7°, θ = 71.5°, θ = 90.0° 04/24/2012 1995DE18 4He(α, γ): excitation function for the decay to the 3 MeV level 33 - 34.7 θlab = 90.0° 04/24/2012 1975NA12 4He(α, γ): excitation function 33 - 36 1 07/19/2011 1977PA26 4He(α, γ): γ

  15. A=12N (1990AJ01)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    90AJ01) (See Energy Level Diagrams for 12N) GENERAL: See also (1985AJ01) and Table Prev. Table 12.22 preview 12.22 [Table of Energy Levels] (in PDF or PS) here. Model calculations:(1984KA1H, 1984SA19). Astrophysical questions:(1985CA41, 1987RA1D, 1988CA26, 1988LE08, 1989KR1C). Applied work:(1987KU17, 1987MI24). Complex reactions involving 12N:(1985NO1E, 1986GA1P, 1987BA1T, 1987RI03, 1988BE02, 1988LE08). Muon and neutrino capture and reactions:(1986DA1J, 1987KR1L, 1988AL1O, 1988BO1X, 1988FU08,

  16. A=13C (59AJ76)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    59AJ76) (See the Energy Level Diagram for 13C) GENERAL: See also Table 13.2 [Table of Energy Levels] (in PDF or PS). Theory: See (AU55, LA55B, DA56G, DE56, KU56, BA57, FR58B, SK58). 1. (a) 6Li(7Li, p)12B Qm = 8.338 Eb = 25.876 (b) 6Li(7Li, n)12C Qm = 20.931 (c) 6Li(7Li, 2n)11C Qm = 2.209 See (NO57A). 2. 7Li(7Li, n)13C Qm = 18.624 See (NO57A). 3. 9Be(α, γ)13C Qm = 10.654 At Eα = 1.60 MeV, the capture cross section is less than 30 μb (AL55C). 4. 9Be(α, n)12C Qm = 5.709 Eb = 10.654 Resonances

  17. A=14O (1976AJ04)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    76AJ04) (See Energy Level Diagrams for 14O) GENERAL: See also (1970AJ04) and Table 14.29 [Table of Energy Levels] (in PDF or PS). Nuclear models: (1973SA30, 1974KU1F). Special reactions involving 14O: (1971AR02, 1973BA81, 1975BA1Q, 1975HU14). Reactions involving pions: (1973CH20, 1973DA37, 1975HU1D, 1975RE01). Other topics: (1970FO1B, 1972AN05, 1972CA37, 1972KU1C, 1973GO1H, 1973KA1H, 1973PA1F, 1973RO1R, 1973SP1A, 1974BO22, 1974KU1F, 1974SE1B, 1974VA24, 1975BU1M). Ground state: (1975BE31). 1.

  18. UNITED STATE% ENGINEER OFFICE I" RaCLI MANHATTAN D' ISTRICT

    Office of Legacy Management (LM)

    =A-; _.__ - ._-.. w-1 -~ ,.. P..*e ,e.arzUE.m ~-~JuTm-&a- . . .~ _ UNITED STATE% ENGINEER OFFICE I" RaCLI MANHATTAN D' ISTRICT RLFSR TO ; I=. 0. eox a ,. STATJON J= N E W YORK, N. Y. ~~~tr.~et h % -j&2 Smg-J E" I, du da r"r~~~~e 4% bP&wQ I tlnatodt 3tut8a ~o~tra~t~~ Q.tYiQlaT,* Erar%by r&08, acthg tdwo*. ttra tm!wi~Qmd in tholr dartost ptmx&b~as t&q, #' pm OLIN tit-h you that yell shtikn, the l_aboq at&M.&~, tada, aatbioerg, ae+nt, JkdUtiar,

  19. Aquantis C-Plane Ocean Current Turbine Project

    SciTech Connect (OSTI)

    Fleming, Alex

    2015-09-16

    The Aquantis 2.5 MW Ocean Current Generation Device technology developed by Dehlsen Associates, LLC (DA) is a derivation of wind power generating technology (a means of harnessing a slow moving fluid) adapted to the ocean environment. The Aquantis Project provides an opportunity for accelerated technological development and early commercialization, since it involves the joining of two mature disciplines: ocean engineering and wind turbine design. The Aquantis Current Plane (C-Plane) technology is an ocean current turbine designed to extract kinetic energy from a current flow. The technology is capable of achieving competitively priced, continuous, base-load, and reliable power generation from a source of renewable energy not before possible in this scale or form.

  20. Effect of temperature on carrier formation efficiency in organic photovoltaic cells

    SciTech Connect (OSTI)

    Moritomo, Yutaka Yonezawa, Kouhei; Yasuda, Takeshi

    2014-08-18

    The internal quantum efficiency (?{sub IQ}) of an organic photovoltaic cell is governed by plural processes. Here, we propose that ?{sub IQ} can be experimentally decomposed into carrier formation (?{sub CF}) and carrier transfer (?{sub CT}) efficiencies. By combining femtosecond time-resolved and electrochemical spectroscopy, we clarified the effect of temperature on ?{sub CF} in a regioregular poly(3-hexylthiophene) (rr-P3HT)/[6,6]-phenyl C{sub 61}-butyric acid methyl ester blend film. We found that ?{sub CF}?(=0.55) at 80?K is the same as that (=0.55) at 300?K. The temperature insensitivity of ?{sub CF} indicates that the electron-hole pairs at the D/A interface are seldom subjected to coulombic binding energy.

  1. A=7B (1979AJ01)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    9AJ01) (See the Isobar Diagram for 7B) GENERAL: See also (1974DA1B, 1974IR04, 1975BE31, 1975BE56, 1976IR1B, 1977SP1B). 1. 10B(3He, 6He)7B Qm = -18.55 A 6He group corresponding to the unbound ground state of 7B has been identified at E(3He) = 50 MeV: M - A (7B) = 27.94 ± 0.10, Γ = 1.4 ± 0.2 MeV. The isobaric quartet mass law would predict M - A = 27.76 ± 0.17 MeV. 7B is unbound with respect to 6Be + p (Q = 2.27), 5Li + 2p (Q = 1.68), 4He + 3p (Q = 3.65). The expected single-particle width is

  2. A=7He (1979AJ01)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    9AJ01) (See the Isobar Diagram for 7He) GENERAL: See also (1974AJ01) and Table 7.1 [Table of Energy Levels] (in PDF or PS). See (1974IR04, 1974TH01, 1975PN1A, 1976TR1A, 1977DO06, 1977SH1C, 1978DA06). 1. 7Li(π-, γ)7He Qm = 128.37 The radiative capture has been observed to the ground state of 7He. The (M1) transition is seen Eγ = 126.6 MeV (1976AL1F). See also (1976TR1A). 2. 7Li(n, p)7He Qm = -10.42 At En = 14.8 MeV a proton group is reported corresponding to 7Heg.s.: Γ < 0.2 MeV

  3. A=7He (1984AJ01)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    4AJ01) (See the Isobar Diagram for 7He) GENERAL: See also (1979AJ01) and Table 7.1 [Table of Energy Levels] (in PDF or PS). Reactions involving pions: (1978FU09, 1979BA1M, 1979PE1C). Hypernuclei: (1978DA1A, 1978SO1A, 1979BU1C, 1981WA1J, 1982KO11). Other topics: (1979BE1H, 1981AV02, 1982AW02, 1982NG01). 1. 7Li(π-, γ)7He Qm = 128.36 See (1979AJ01). 2. 7Li(n, p)7He Qm = -10.42 At En = 14.8 MeV a proton group is reported corresponding to 7Heg.s.: Γ < 0.2 MeV: see (1979AJ01). See also

  4. A=7He (1988AJ01)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    8AJ01) (See the Isobar Diagram for 7He) GENERAL: See also (1984AJ01) and Table 7.1 [Table of Energy Levels] (in PDF or PS). Hypernuclei: (1982KA1D, 1983FE07, 1984AS1D, 1985KO1G, 1986DA1B, 1986DO01, 1986ME1F). Other topics: (1983ANZQ, 1984FR13, 1984VA06, 1986GI10, 1986SH1L, 1987BO40, 1987GOZN, 1987PE1C). Mass of 7He: The atomic mass excess of 7He is 26.11 ± 0.03 MeV: 7He is then unbound with respect to decay into 6He + n by 0.44 MeV: see (1984AJ01). The ground state is calculated to have Jπ =

  5. A=8He (1988AJ01)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    8AJ01) (See the Isobar Diagram for 8He) GENERAL: See also (1984AJ01) and Table 8.1 [Table of Energy Levels] (in PDF or PS). Model calculations: (1984VA06, 1985PO10, 1987BL18). Complex reactions involving 8He: (1982AL33, 1983AN13, 1985MA13, 1985TA1D, 1986SA30, 1987AR1G, 1987BO40, 1987KO1Z, 1987PE1C, 1987TAZU, 1988GA10, 1988ST06, 1988TA1A). Hypernuclei: (1982KA1D, 1983DO1B, 1984BO1H, 1985AH1A, 1985IK1A, 1986BA1W, 1986DA1B, 1987MI38, 1987PO1H). Other topics: (1983GL1B, 1985AN28, 1987AJ1A,

  6. Localization and physical mapping of the prostate-specific membrane antigen (PSM) gene to human chromosome 11

    SciTech Connect (OSTI)

    Rinker-Schaeffer, C.W.; Hawkins, A.L.; Griffin, C.A.; Isaacs, J.T. [Johns Hopkins Medical School, Baltimore, MD (United States)] [and others

    1995-11-01

    The prostate-specific membrane antigen (PSM) was identified by the monoclonal antibody 7E11-C5.3, which was raised against the human prostatic carcinoma cell line LNCaP. The PSM antigen is expressed by normal, neoplastic, and metastatic prostatic tissues. The 2.65-kb cDNA encoding the 100-kDa PSM glycoprotein was cloned from LNCaP cells. Studies have shown that the expression of PSM is tissue-specific. In the present study monochromosomal somatic cell hybrids were used to localize the PSM gene to human chromosome 11. Using this information, initial mapping studies identified two potential PSM gene loci at 11p11.1-p13 and 11q14. Further high-stringency analysis using cosmid probes identified the 11q14 region as the location of the PSM gene. 10 refs., 2 figs.

  7. Ultra High Mass Range Mass Spectrometer System

    DOE Patents [OSTI]

    Reilly, Peter T. A. [Knoxville, TN

    2005-12-06

    Applicant's present invention comprises mass spectrometer systems that operate in a mass range from 1 to 10.sup.16 DA. The mass spectrometer system comprising an inlet system comprising an aerodynamic lens system, a reverse jet being a gas flux generated in an annulus moving in a reverse direction and a multipole ion guide; a digital ion trap; and a thermal vaporization/ionization detector system. Applicant's present invention further comprises a quadrupole mass spectrometer system comprising an inlet system having a quadrupole mass filter and a thermal vaporization/ionization detector system. Applicant's present invention further comprises an inlet system for use with a mass spectrometer system, a method for slowing energetic particles using an inlet system. Applicant's present invention also comprises a detector device and a method for detecting high mass charged particles.

  8. 18O Cross Section

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    α, X) (Current as of 05/14/2012) NSR Reaction Eα (MeV) Cross Section File X4 Dataset Date Added 2003DA19 18O(α, γ): deduced resonance strengths ~ 470 - 770 keV X4 02/13/2012 1978TR05 18O(α, γ): excitation function for the 1.27 MeV secondary γ-ray transition 0.6 - 2.3 θγ = 0° 02/29/2012 1990VO06 18O(α, γ): resonance γ yields < 0.78 X4 02/13/2012 1973BA10 18O(α, n): σ with target thickness 1 - 5 6 keV, 13 keV 06/06/2011 1956BO61 18O(α, n): neutron yields 1.8 - 5.3 0° - 30° X4

  9. Closing the Carbon Balance for Fermentation by Clostridium thermocellum (ATCC 27405)

    SciTech Connect (OSTI)

    Ellis, Lucas D; Holwerda, Evert K; Hogsett, David; Rogers, Steve; Shao, Xiongjun; Tschaplinski, Timothy J; Thorne, Phil; Lynd, L.

    2012-01-01

    Our lab and most others have not been able to close a carbon balance for fermentation by the thermophilic, cellulolytic anaerobe, Clostridium thermocellum. We undertook a detailed accounting of product formation in C. thermocellum ATCC 27405. Elemental analysis revealed that for both cellulose (Avicel) and cellobiose, {>=}92% of the substrate carbon utilized could be accounted for in the pellet, supernatant and off-gas when including sampling. However, 11.1% of the original substrate carbon was found in the liquid phase and not in the form of commonly-measured fermentation products - ethanol, acetate, lactate, and formate. Further detailed analysis revealed all the products to be <720 da and have not usually been associated with C. thermocellum fermentation, including malate, pyruvate, uracil, soluble glucans, and extracellular free amino acids. By accounting for these products, 92.9% and 93.2% of the final product carbon was identified during growth on cellobiose and Avicel, respectively.

  10. An ion mobility mass spectrometer for investigating photoisomerization and photodissociation of molecular ions

    SciTech Connect (OSTI)

    Adamson, B. D.; Coughlan, N. J. A.; Markworth, P. B.; Bieske, E. J.; Continetti, R. E.

    2014-12-15

    An ion mobility mass spectrometry apparatus for investigating the photoisomerization and photodissociation of electrosprayed molecular ions in the gas phase is described. The device consists of a drift tube mobility spectrometer, with access for a laser beam that intercepts the drifting ion packet either coaxially or transversely, followed by a quadrupole mass filter. An ion gate halfway along the drift region allows the instrument to be used as a tandem ion mobility spectrometer, enabling mobility selection of ions prior to irradiation, with the photoisomer ions being separated over the second half of the drift tube. The utility of the device is illustrated with photoisomerization and photodissociation action spectra of carbocyanine molecular cations. The mobility resolution of the device for singly charged ions is typically 80 and it has a mass range of 100-440 Da, with the lower limit determined by the drive frequency for the ion funnels, and the upper limit by the quadrupole mass filter.

  11. Alterações Climáticas

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Manter as Alterações Climáticas Debaixo de Olho Sabes a diferença entre o "clima" e o "estado do tempo"? O "estado do tempo" são as condições que vês e sentes fora da tua janela neste momento; talvez frio, nublado e com chuva, ou então quente e com sol, com céu descoberto e azul. O clima é como o "estado do tempo", excepto o facto de abranger um longo período de tempo ... meses, anos ou até mesmo décadas. O clima é a combinação de

  12. HEAD INJURY ASSESSMENT IN JUVENILE CHINOOK USING THE ALPHA II-SPECTRIN BIOMARKER: EFFECTS OF PRESSURE CHANGES AND PASSAGE THROUGH A REMOVABLE SPILLWAY WEIR

    SciTech Connect (OSTI)

    Jonason, C.; Miracle, A.

    2009-01-01

    The cytoskeletal protein alpha II-spectrin has specifi c neurodegenerative mechanisms that allow the necrotic (injury-induced) and apoptotic (non-injury-induced) pathways of proteolysis to be differentiated in an immunoblot. Consequently, ?II-spectrin breakdown products (SBDPs) are potential biomarkers for diagnosing traumatic brain injury (TBI). The purpose of the following investigation, consisting of two studies, was to evaluate the utility of the spectrin biomarker in diagnosing TBI in fi sh that travel through hydroelectric dams in the Columbia and Snake Rivers. The fi rst study used hyperbaric pressure chambers to simulate the pressure changes that affect fi sh during passage through a Federal Columbia River Power System (FCRPS) Kaplan turbine. The second study tested the effect of a removable spillway weir (RSW) on the passage of juvenile chinook (Oncorhynchus tshawytscha). This study was conducted in tandem with a balloon-tag study by the U.S. Army Corps of Engineers. Brain samples from fi sh were collected and analyzed using an immunoblot for SBDPs, and imaging software was used to quantify the protein band density and determine the ratio of cleaved protein to total protein. The biomarker analyses found higher SBDP expression levels in fi sh that were exposed to lower pressure nadirs and fi sh that passed through the RSW at a deep orientation. In general, the incidence of injuries observed after treatment positively correlated with expression levels, suggesting that the biomarker method of analysis is comparable to traditional methods of injury assessment. It was also found that, for some treatments, the 110 kDa spectrin fragment (SBDP 110) correlated more strongly with necrotic head injury incidence and mortality rates than did the total cleaved protein or the 120 kDa fragment. These studies will be informative in future decisions regarding the design of turbines and fi sh passage structures in hydroelectric dams and will hopefully contribute to the development of faster and more accurate techniques for diagnosing TBI in fi sh.

  13. Multipinhole collimator with 20 apertures for a brain SPECT application

    SciTech Connect (OSTI)

    Lee, Tzu-Cheng; Ellin, Justin R.; Shrestha, Uttam; Seo, Youngho; Huang, Qiu; Gullberg, Grant T.

    2014-11-01

    Purpose: Several new technologies for single photon emission computed tomography (SPECT) instrumentation with parallel-hole collimation have been proposed to improve detector sensitivity and signal collection efficiency. Benefits from improved signal efficiency include shorter acquisition times and lower dose requirements. In this paper, the authors show a possibility of over an order of magnitude enhancement in photon detection efficiency (from 7.6 × 10{sup −5} to 1.6 × 10{sup −3}) for dopamine transporter (DaT) imaging of the striatum over the conventional SPECT parallel-hole collimators by use of custom-designed 20 multipinhole (20-MPH) collimators with apertures of 0.75 cm diameter. Methods: Quantifying specific binding ratio (SBR) of {sup 123}I-ioflupane or {sup 123}I-iometopane’s signal at the striatal region is a common brain imaging method to confirm the diagnosis of the Parkinson’s disease. The authors performed imaging of a striatal phantom filled with aqueous solution of I-123 and compared camera recovery ratios of SBR acquired between low-energy high-resolution (LEHR) parallel-hole collimators and 20-MPH collimators. Results: With only two-thirds of total acquisition time (20 min against 30 min), a comparable camera recovery ratio of SBR was achieved using 20-MPH collimators in comparison to that from the LEHR collimator study. Conclusions: Their systematic analyses showed that the 20-MPH collimator could be a promising alternative for the DaT SPECT imaging for brain over the traditional LEHR collimator, which could give both shorter scan time and improved diagnostic accuracy.

  14. Monitoring, Controlling and Safeguarding Radiochemical Streams at Spent Fuel Reprocessing Facilities, Part 2: Gamma-Ray Spectroscopic Methods

    SciTech Connect (OSTI)

    Schwantes, Jon M.; Bryan, Samuel A.; Orton, Christopher R.; Levitskaia, Tatiana G.; Fraga, Carlos G.

    2012-02-10

    The International Atomic Energy Agency (IAEA) has established international safeguards standards for fissionable material at spent fuel reprocessing plants to ensure that significant quantities of weapons-useable nuclear material are not diverted from these facilities. For large throughput nuclear facilities, it is difficult to satisfy the IAEA safeguards accountancy goal for detection of abrupt diversion. Currently, methods to verify material control and accountancy (MC&A) at these facilities require time-consuming and resource-intensive destructive assay (DA). Leveraging new on-line non-destructive assay (NDA) process monitoring techniques in conjunction with the traditional and highly precise DA methods may provide an additional measure to nuclear material accountancy which would potentially result in a more timely, cost-effective and resource efficient means for safeguards verification at such facilities. By monitoring process control measurements (e.g. flowrates, temperatures, or concentrations of reagents, products or wastes), abnormal plant operations can be detected. Pacific Northwest National Laboratory (PNNL) is developing on-line NDA process monitoring technologies based upon gamma-ray and optical spectroscopic measurements to potentially reduce the time and resource burden associated with current techniques. The Multi-Isotope Process (MIP) Monitor uses gamma spectroscopy and multivariate analysis to identify off-normal conditions in process streams. The spectroscopic monitor continuously measures chemical compositions of the process streams including actinide metal ions (U, Pu, Np), selected fission products, and major stable flowsheet reagents using UV-Vis, Near IR and Raman spectroscopy. Multi-variate analysis is also applied to the optical measurements in order to quantify concentrations of analytes of interest within a complex array of radiochemical streams. This paper will provide an overview of these methods and reports on-going efforts to develop and demonstrate the technologies. This paper is Part 2 of a two part series, and focuses on the gamma spectroscopy based, Multi-Isotope Process (MIP) Monitor method.

  15. Defining the needs for gas centrifuge enrichment plants advanced safeguards

    SciTech Connect (OSTI)

    Boyer, Brian David; Erpenbeck, Heather H; Miller, Karen A; Swinhoe, Martyn T; Ianakiev, Kiril; Marlow, Johnna B

    2010-04-05

    Current safeguards approaches used by the International Atomic Energy Agency (IAEA) at gas centrifuge enrichment plants (GCEPs) need enhancement in order to verify declared low-enriched (LEU) production, detect undeclared LEU production and detect highly enriched uranium (HEU) production with adequate detection probability using nondestructive assay (NDA) techniques. At present inspectors use attended systems, systems needing the presence of an inspector for operation, during inspections to verify the mass and {sup 235}U enrichment of declared UF{sub 6} containers used in the process of enrichment at GCEPs. In verifying declared LEU production, the inspectors also take samples for off-site destructive assay (DA) which provide accurate data, with 0.1% to 0.5% measurement uncertainty, on the enrichment of the UF{sub 6} feed, tails, and product. However, taking samples of UF{sub 6} for off-site analysis is a much more labor and resource intensive exercise for the operator and inspector. Furthermore, the operator must ship the samples off-site to the IAEA laboratory which delays the timeliness of results and interruptions to the continuity of knowledge (CofK) of the samples during their storage and transit. This paper contains an analysis of possible improvements in unattended and attended NDA systems such as process monitoring and possible on-site analysis of DA samples that could reduce the uncertainty of the inspector's measurements and provide more effective and efficient IAEA GCEPs safeguards. We also introduce examples advanced safeguards systems that could be assembled for unattended operation.

  16. Monitoring, Controlling and Safeguarding Radiochemical Streams at Spent Fuel Reprocessing Facilities, Part 1: Optical Spectroscopic Methods

    SciTech Connect (OSTI)

    Bryan, Samuel A.; Levitskaia, Tatiana G.; Schwantes, Jon M.; Orton, Christopher R.; Peterson, James M.; Casella, Amanda J.

    2012-02-07

    Abstract: The International Atomic Energy Agency (IAEA) has established international safeguards standards for fissionable material at spent fuel reprocessing plants to ensure that significant quantities of weapons-useable nuclear material are not diverted from these facilities. For large throughput nuclear facilities, it is difficult to satisfy the IAEA safeguards accountancy goal for detection of abrupt diversion. Currently, methods to verify material control and accountancy (MC&A) at these facilities require time-consuming and resource-intensive destructive assay (DA). Leveraging new on-line non-destructive assay (NDA) process monitoring techniques in conjunction with the traditional and highly precise DA methods may provide an additional measure to nuclear material accountancy which would potentially result in a more timely, cost-effective and resource efficient means for safeguards verification at such facilities. By monitoring process control measurements (e.g. flowrates, temperatures, or concentrations of reagents, products or wastes), abnormal plant operations can be detected. Pacific Northwest National Laboratory (PNNL) is developing on-line NDA process monitoring technologies based upon gamma-ray and optical spectroscopic measurements to potentially reduce the time and resource burden associated with current techniques. The Multi-Isotope Process (MIP) Monitor uses gamma spectroscopy and multivariate analysis to identify off-normal conditions in process streams. The spectroscopic monitor continuously measures chemical compositions of the process streams including actinide metal ions (U, Pu, Np), selected fission products, and major stable flowsheet reagents using UV-Vis, Near IR and Raman spectroscopy. Multi-variate analysis is also applied to the optical measurements in order to quantify concentrations of analytes of interest within a complex array of radiochemical streams. This paper will provide an overview of these methods and reports on-going efforts to develop and demonstrate the technologies. This paper is Part 1 of a two part series, and focuses on the optical spectroscopy based process monitoring methods.

  17. Monitoring, Controlling and Safeguarding Radiochemical Streams at Spent Fuel Reprocessing Facilities with Optical and Gamma-Ray Spectroscopic Methods

    SciTech Connect (OSTI)

    Schwantes, Jon M.; Bryan, Samuel A.; Orton, Christopher R.; Levitskaia, Tatiana G.; Fraga, Carlos G.

    2012-11-06

    The International Atomic Energy Agency (IAEA) has established international safeguards standards for fissionable material at spent fuel reprocessing plants to ensure that significant quantities of weapons-useable nuclear material are not diverted from these facilities. For large throughput nuclear facilities, it is difficult to satisfy the IAEA safeguards accountancy goal for detection of abrupt diversion. Currently, methods to verify material control and accountancy (MC&A) at these facilities require time-consuming and resourceintensive destructive assay (DA). Leveraging new on-line non-destructive assay (NDA) process monitoring techniques in conjunction with the traditional and highly precise DA methods may provide an additional measure to nuclear material accountancy which would potentially result in a more timely, cost-effective and resource efficient means for safeguards verification at such facilities. By monitoring process control measurements (e.g. flowrates, temperatures, or concentrations of reagents, products or wastes), abnormal plant operations can be detected. Pacific Northwest National Laboratory (PNNL) is developing on-line NDA process monitoring technologies based upon gamma-ray and optical spectroscopic measurements to potentially reduce the time and resource burden associated with current techniques. The Multi-Isotope Process (MIP) Monitor uses gamma spectroscopy and multivariate analysis to identify offnormal conditions in process streams. The spectroscopic monitor continuously measures chemical compositions of the process streams including actinide metal ions (U, Pu, Np), selected fission products, and major stable flowsheet reagents using UV-Vis, Near IR and Raman spectroscopy. Multi-variate analysis is also applied to the optical measurements in order to quantify concentrations of analytes of interest within a complex array of radiochemical streams. This paper will provide an overview of these methods and reports on-going efforts to develop and demonstrate the technologies.

  18. Advanced Process Monitoring Techniques for Safeguarding Reprocessing Facilities

    SciTech Connect (OSTI)

    Orton, Christopher R.; Bryan, Samuel A.; Schwantes, Jon M.; Levitskaia, Tatiana G.; Fraga, Carlos G.; Peper, Shane M.

    2010-11-30

    The International Atomic Energy Agency (IAEA) has established international safeguards standards for fissionable material at spent fuel reprocessing plants to ensure that significant quantities of weapons-grade nuclear material are not diverted from these facilities. For large throughput nuclear facilities, it is difficult to satisfy the IAEA safeguards accountancy goal for detection of abrupt diversion. Currently, methods to verify material control and accountancy (MC&A) at these facilities require time-consuming and resource-intensive destructive assay (DA). Leveraging new on-line non destructive assay (NDA) process monitoring techniques in conjunction with the traditional and highly precise DA methods may provide an additional measure to nuclear material accountancy which would potentially result in a more timely, cost-effective and resource efficient means for safeguards verification at such facilities. By monitoring process control measurements (e.g. flowrates, temperatures, or concentrations of reagents, products or wastes), abnormal plant operations can be detected. Pacific Northwest National Laboratory (PNNL) is developing on-line NDA process monitoring technologies, including both the Multi-Isotope Process (MIP) Monitor and a spectroscopy-based monitoring system, to potentially reduce the time and resource burden associated with current techniques. The MIP Monitor uses gamma spectroscopy and multivariate analysis to identify off-normal conditions in process streams. The spectroscopic monitor continuously measures chemical compositions of the process streams including actinide metal ions (U, Pu, Np), selected fission products, and major cold flowsheet chemicals using UV-Vis, Near IR and Raman spectroscopy. This paper will provide an overview of our methods and report our on-going efforts to develop and demonstrate the technologies.

  19. TOWARD A SPECTROSCOPIC CENSUS OF WHITE DWARFS WITHIN 40 pc OF THE SUN

    SciTech Connect (OSTI)

    Limoges, M.-M.; Bergeron, P.; Lepine, S. E-mail: bergeron@astro.umontreal.ca

    2013-05-15

    We present the preliminary results of a survey aimed at significantly increasing the range and completeness of the local census of spectroscopically confirmed white dwarfs. The current census of nearby white dwarfs is reasonably complete only to about 20 pc of the Sun, a volume that includes around 130 white dwarfs, a sample too small for detailed statistical analyses. This census is largely based on follow-up investigations of stars with very large proper motions. We describe here the basis of a method that will lead to a catalog of white dwarfs within 40 pc of the Sun and north of the celestial equator, thus increasing by a factor of eight the extent of the northern sky census. White dwarf candidates are identified from the SUPERBLINK proper motion database, allowing us to investigate stars down to a proper motion limit {mu} > 40 mas yr{sup -1}, while minimizing the kinematic bias for nearby objects. The selection criteria and distance estimates are based on a combination of color-magnitude and reduced proper motion diagrams. Our follow-up spectroscopic observation campaign has so far uncovered 193 new white dwarfs, among which we identify 127 DA (including 9 DA+dM and 4 magnetic), 1 DB, 56 DC, 3 DQ, and 6 DZ stars. We perform a spectroscopic analysis on a subsample of 84 DAs, and provide their atmospheric parameters. In particular, we identify 11 new white dwarfs with spectroscopic distances within 25 pc of the Sun, including five candidates to the D < 20 pc subset.

  20. Fatigue and Creep Crack Propagation behaviour of Alloy 617 in the Annealed and Aged Conditions

    SciTech Connect (OSTI)

    Julian K. Benz; Richard N. Wright

    2013-10-01

    The crack propagation behaviour of Alloy 617 was studied under various conditions. Elevated temperature fatigue and creep-fatigue crack growth experiments were conducted at 650 and 800 degrees C under constant stress intensity (triangle K) conditions and triangular or trapezoidal waveforms at various frequencies on as-received, aged, and carburized material. Environmental conditions included both laboratory air and characteristic VHTR impure helium. As-received Alloy 617 displayed an increase in the crack growth rate (da/dN) as the frequency was decreased in air which indicated a time-dependent contribution component in fatigue crack propagation. Material aged at 650°C did not display any influence on the fatigue crack growth rates nor the increasing trend of crack growth rate with decreasing frequency even though significant microstructural evolution, including y’ (Ni3Al) after short times, occurred during aging. In contrast, carburized Alloy 617 showed an increase in crack growth rates at all frequencies tested compared to the material in the standard annealed condition. Crack growth studies under quasi-constant K (i.e. creep) conditions were also completed at 650 degrees C and a stress intensity of K = 40 MPa9 (square root)m. The results indicate that crack growth is primarily intergranular and increased creep crack growth rates exist in the impure helium environment when compared to the results in laboratory air. Furthermore, the propagation rates (da/dt) continually increased for the duration of the creep crack growth either due to material aging or evolution of a crack tip creep zone. Finally, fatigue crack propagation tests at 800 degrees C on annealed Alloy 617 indicated that crack propagation rates were higher in air than impure helium at the largest frequencies and lowest stress intensities. The rates in helium, however, eventually surpass the rates in air as the frequency is reduced and the stress intensity is decreased which was not observed at 650 degrees C.

  1. Final Progress Report: Coupled Biogeochemical Process Evaluation for Conceptualizing Trichloroethylene Cometabolism

    SciTech Connect (OSTI)

    Crawford, Ronald L; Paszczynski, Andrzej J

    2010-02-19

    Our goal within the overall project is to demonstrate the presence and abundance of methane monooxygenases (MMOs) enzymes and their genes within the microbial community of the Idaho National Laboratory (INL) Test Area North (TAN) site. MMOs are thought to be the primary catalysts of natural attenuation of trichloroethylene (TCE) in contaminated groundwater at this location. The actual presence of the proteins making up MMO complexes would provide direct evidence for its participation in TCE degradation. The quantitative estimation of MMO genes and their translation products (sMMO and pMMO proteins) and the knowledge about kinetics and substrate specificity of MMOs will be used to develop mathematical models of the natural attenuation process in the TAN aquifer. The model will be particularly useful in prediction of TCE degradation rate in TAN and possibly in the other DOE sites. Bacteria known as methanotrophs produce a set of proteins that assemble to form methane monooxygenase complexes (MMOs), enzymes that oxidize methane as their natural substrate, thereby providing a carbon and energy source for the organisms. MMOs are also capable of co-metabolically transforming chlorinated solvents like TCE into nontoxic end products such as carbon dioxide and chloride. There are two known forms of methane monooxygenase, a membrane-bound particulate form (pMMO) and a cytoplasmic soluble form (sMMO). pMMO consists of two components, pMMOH (a hydroxylase comprised of 47-, 27-, and 24-kDa subunits) and pMMOR (a reductase comprised of 63 and 8-kDa subunits). sMMO consists of three components: a hydroxylase (protein A-250 kDa), a dimer of three subunits (α2β2γ2), a regulatory protein (protein B-15.8 kDa), and a reductase (protein C-38.6 kDa). All methanotrophs will produce a methanol dehydrogenase to channel the product of methane oxidation (methanol) into the central metabolite formaldehyde. University of Idaho (UI) efforts focused on proteomic analyses using mass spectrometry and genomic analyses using RT-PCR to characterize these enzyme systems. UI’s specific objectives were to develop the proteomics and genomic tools to assess the presence of the methane monooxygenase (MMO) proteins in the aquifers under study and relate this to the enumeration of methanotrophic microorganisms. We targeted the identification of both sMMO and pMMO. We believe that the copper level in the TAN aquifer is most likely suppressing the expression of sMMO and mediates the higher levels of pMMO expression. Hence our investigations included the identification of both forms of MMOs, and we expected a higher concentration of pMMO proteins in TAN samples. The amounts of these proteins present were correlated with numbers of methanotrophs determined by us and other members of the research team using PCR-based methods. In summary, to accomplish our objectives we applied environmental proteomics techniques to monitor proteins that are involved in the co-metabolic degradation of trichloroethylene (TCE) in groundwater of the INL TAN site on Department of Energy ands of near Idaho Falls, ID USA. To acquire peptides sequences information we used an ultra performance chromatography (UPLC) system coupled with QToF Premiere nano-electrospray tandem quadropole-time of flight mass spectrometer. Our goal was to identify signature peptides of methane monooxygenases (MMOs) within methanotrophic bacteria that are active in cometabolic degradation of TCE. We developed a new method for extracting total proteins from environmental planktonic and/or biofilm samples that involve a new time course cell lysis and protein extraction method in combination with chromatographic separation of peptide and tandem mass spectrometry sequencing. The techniques resulted in successful extraction and identification of MMO-based peptides from both pure cultures and TAN site samples. The work confirmed the importance of mathonotrophs in the co-metabolic removal of TCE from the TAN site aquifer.

  2. Structure of the Archaeoglobus fulgidus orphan ORF AF1382 determined by sulfur SAD from a moderately diffracting crystal

    SciTech Connect (OSTI)

    Zhu, Jin-Yi; Fu, Zheng-Qing; Chen, Lirong; Xu, Hao; Chrzas, John; Rose, John Wang, Bi-Cheng

    2012-09-01

    The crystal structure of the 11.14 kDa orphan ORF 1382 from Archaeoglobus fulgidus (AF1382) has been determined by sulfur SAD phasing using data collected from a moderately diffracting crystal and 1.9 synchrotron X-rays. The crystal structure of the 11.14 kDa orphan ORF 1382 from Archaeoglobus fulgidus (AF1382) has been determined by sulfur SAD phasing using a moderately diffracting crystal and 1.9 wavelength synchrotron X-rays. AF1382 was selected as a structural genomics target by the Southeast Collaboratory for Structural Genomics (SECSG) since sequence analyses showed that it did not belong to the Pfam-A database and thus could represent a novel fold. The structure was determined by exploiting longer wavelength X-rays and data redundancy to increase the anomalous signal in the data. AF1382 is a 95-residue protein containing five S atoms associated with four methionine residues and a single cysteine residue that yields a calculated Bijvoet ratio (?F{sub anom}/F) of 1.39% for 1.9 wavelength X-rays. Coupled with an average Bijvoet redundancy of 25 (two 360 data sets), this produced an excellent electron-density map that allowed 69 of the 95 residues to be automatically fitted. The S-SAD model was then manually completed and refined (R = 23.2%, R{sub free} = 26.8%) to 2.3 resolution. High-resolution data were subsequently collected from a better diffracting crystal using 0.97 wavelength synchrotron X-rays and the S-SAD model was refined (R = 17.9%, R{sub free} = 21.4%) to 1.85 resolution. AF1382 has a winged-helixturnhelix structure common to many DNA-binding proteins and most closely resembles the N-terminal domain (residues 182) of the Rio2 kinase from A. fulgidus, which has been shown to bind DNA, and a number of MarR-family transcriptional regulators, suggesting a similar DNA-binding function for AF1382. The analysis also points out the advantage gained from carrying out data reduction and structure determination on-site while the crystal is still available for further data collection.

  3. Intergranular stress corrosion cracking initiation and growth in mill-annealed Alloy 600 tubing in high-temperature caustic

    SciTech Connect (OSTI)

    Brisson, B.W.; Ballinger, R.G.; McIlree, A.R.

    1998-07-01

    Historically, pressurized water reactor (PWR) steam generator (SG) reliability has been dominated by degradation of alloy 600 (UNS N06600) tubing material. Stress corrosion cracking (SCC) crack initiation and crack growth rates (CGR) were measured in mill-annealed alloy 600 (UNS N06600) tubing as a function of the stress intensity factor (K) in 10% caustic at 315 C. Tests were conducted using internally pressurized smooth and precracked tubing. Samples were polarized to 150 mV (precracked tube test) or 225 mV (initiation test) with respect to a nickel electrode. Crack initiation and growth from the external tube surface were monitored using a multifrequency alternating current (AC) potential drop system. The AC potential drop system allowed detection of initiation from a smooth surface as well as the monitoring of crack extension in real time. In the case of precracked sample tests, the sample was precracked in fatigue from a sharp v-notch. CGR were obtained over the K range between 4 MPa{radical}m and 18 MPa{radical}m. Values for K were estimated based upon fractographic analysis of samples after testing and an estimate of the K-solution for a thin-walled tube. Average CGR ranged from 2 mm/y to 14 mm/y. CGR determined in this investigation represent the first SCC CGR data obtained in high-temperature caustic using actual steam generator tubing. Growth rates obtained fell within the overall range of the existing database for CGR (da/dt) in alloy 600. The data and analysis suggested a threshold value of K for K-driven crack growth of {approx} 4 MPa{radical}m. However, since the scatter in existing data is very large and the conditions for most of the data are poorly known or not known at all, this was surprising. More importantly, from the standpoint of life prediction, it was observed that da/dt responded to and was a function of K for cracks as small as 0.2 mm in depth, and probably smaller.

  4. Toward a Fieldable Atomic Mass Spectrometer for Safeguards Applications: Sample Preparation and Ionization

    SciTech Connect (OSTI)

    Barinaga, Charles J.; Hager, George J.; Hart, Garret L.; Koppenaal, David W.; Marcus, R. Kenneth; Jones, Sarah MH; Manard, Benjamin T.

    2014-10-31

    The International Atomic Energy Agency’s (IAEA’s) long-term research and development plan calls for the development of new methods to detect misuse at nuclear fuel cycle facilities such as reprocessing and enrichment plants. At enrichment plants, for example, the IAEA’s contemporary safeguards approaches are based on a combination of routine and random inspections that include collection of UF6 samples from in-process material and selected cylinders for subsequent analyses. These analyses include destructive analysis (DA) in a laboratory (typically by mass spectrometry [MS]) for isotopic characterization, and environmental sampling (ES) for subsequent laboratory elemental and isotopic analysis (also both typically by MS). One area of new method development includes moving this kind of isotope ratio analytical capability for DA and ES activities into the field. Some of the reasons for these developments include timeliness of results, avoidance of hazardous material shipments, and guidance for additional sample collecting. However, this capability does not already exist for several reasons, such as that most lab-based chemical and instrumental methods rely on laboratory infrastructure (highly trained staff, power, space, hazardous material handling, etc.) and require significant amounts of consumables (power, compressed gases, etc.). In addition, there are no currently available, fieldable instruments for atomic or isotope ratio analysis. To address these issues, Pacific Northwest National Laboratory (PNNL) and collaborator, Clemson University, are studying key areas that limit the fieldability of isotope ratio mass spectrometry for atomic ions: sample preparation and ionization, and reducing the physical size of a fieldable mass spectrometer. PNNL is seeking simple and robust techniques that could be effectively used by inspectors who may have no expertise in analytical MS. In this report, we present and describe the preliminary findings for three candidate techniques: matrix-assisted laser desorption/ionization (MALDI) MS, liquid sampling-atmospheric pressure glow discharge (LS-APGD), and laser ablation/ionization (LAI) MS at atmospheric pressure. Potential performance metrics for these techniques will be presented, including detectability, response, isotope ratio accuracy and precision, and ease of use.

  5. Safeguards Verification Measurements using Laser Ablation, Absorbance Ratio Spectrometry in Gaseous Centrifuge Enrichment Plants

    SciTech Connect (OSTI)

    Anheier, Norman C.; Cannon, Bret D.; Qiao, Hong; Phillips, Jon R.

    2012-07-01

    Laser Ablation Absorbance Ratio Spectrometry (LAARS) is a new verification measurement technology under development at the US Department of Energy’s (DOE) Pacific Northwest National Laboratory (PNNL). LAARS uses three lasers to ablate and then measure the relative isotopic abundance of uranium compounds. An ablation laser is tightly focused on uranium-bearing solids producing a small plume containing uranium atoms. Two collinear wavelength-tuned spectrometry lasers transit through the plume and the absorbance of U-235 and U-238 isotopes are measured to determine U-235 enrichment. The measurement has high relative precision and detection limits approaching the femtogram range for uranium. It is independent of chemical form and degree of dilution with nuisance dust and other materials. High speed sample scanning and pinpoint characterization allow measurements on millions of particles/hour to detect and analyze the enrichment of trace uranium in samples. The spectrometer is assembled using commercially available components at comparatively low cost, and features a compact and low power design. Future designs can be engineered for reliable, autonomous deployment within an industrial plant environment. Two specific applications of the spectrometer are under development: 1) automated unattended aerosol sampling and analysis and 2) on-site small sample destructive assay measurement. The two applications propose game-changing technological advances in gaseous centrifuge enrichment plant (GCEP) safeguards verification. The aerosol measurement instrument, LAARS-environmental sampling (ES), collects aerosol particles from the plant environment in a purpose-built rotating drum impactor and then uses LAARS-ES to quickly scan the surface of the impactor to measure the enrichments of the captured particles. The current approach to plant misuse detection involves swipe sampling and offsite analysis. Though this approach is very robust it generally requires several months to obtain results from a given sample collection. The destructive assay instrument, LAARS-destructive assay (DA), uses a simple purpose-built fixture with a sampling planchet to collect adsorbed UF6 gas from a cylinder valve or from a process line tap or pigtail. A portable LAARS-DA instrument scans the microgram quantity of uranium collected on the planchet and the assay of the uranium is measured to ~0.15% relative precision. Currently, destructive assay samples for bias defect measurements are collected in small sample cylinders for offsite mass spectrometry measurement.

  6. Expression of low-, intermediate-, and high-affinity IL-2 receptors on B cell lines derived from patients with undifferentiated lymphoma of Burkitt's and non-Burkitt's types

    SciTech Connect (OSTI)

    Benjamin, D.; Rosolen, A.; Wormsley, S.B.; DeBault, L.E.; Colamonici, O.R. )

    1990-08-01

    IL-2 receptors on T cells exist in at least three forms which differ in their ligand-binding affinity. The low-affinity IL-2 receptor (IL-2R) consists of the 55-kDa Tac protein (p55 alpha), the intermediate-affinity site corresponds to the 70-kDa molecule (p70 beta), and the high-affinity IL-2R consists of a noncovalent heterodimeric structure involving both p55 alpha and p70 beta. We studied 24 B cell lines (8 EBV-negative and 16 EBV-positive) for IL-2R expression in the presence or absence of the tumor promoter, teleocidin. 125I-IL-2 radioreceptor binding assays and crosslinking studies demonstrated the sole expression of p55 alpha in EBV-negative cell lines only, whereas p55 alpha present in EBV-positive cell lines was always associated with p70 beta to construct high-affinity IL-2R. p70 beta was not detected in any of the EBV-negative cell lines, but was expressed on most of the EBV-positive cell lines (13 of 16). Our data also indicate that the expression of p55 alpha and p70 beta by radiolabeling correlates with their expression in flow cytometry, and that a large excess of p55 alpha is required to construct high-affinity IL-2R. Coexpression of p55 alpha and p70 beta on human B cells contributed to constructing high-affinity IL-2R hybrid complex as shown by rapid association rate contributed by p55 alpha and slow dissociation rate by p70 beta; teleocidin's ability to induce p55 alpha on cell lines which express p70 beta only, resulting in appearance of high-affinity IL-2R; and blocking p55 alpha by anti-Tac mAb in cell lines which constitutively express high-affinity IL-2R eliminated both high- and low-affinity components. The existence of low, intermediate, and high IL-2R on human B cells bears important future implications for understanding the mechanism of IL-2 signaling and the role of IL-2 in B cell activation, proliferation, and differentiation.

  7. Crystal structure of 3,4-dihydroxy-2-butanone 4-phosphate synthase of riboflavin biosynthesis

    SciTech Connect (OSTI)

    Liao, D.-I.; Calabrese, J.C.; Wawrzak, Z.; Viitanen, P.V.; Jordan, D.B.

    2010-03-05

    3,4-Dihydroxy-2-butanone-4-phosphate synthase catalyzes a commitment step in the biosynthesis of riboflavin. On the enzyme, ribulose 5-phosphate is converted to 3,4-dihydroxy-2-butanone 4-phosphate and formate in steps involving enolization, ketonization, dehydration, skeleton rearrangement, and formate elimination. The enzyme is absent in humans and an attractive target for the discovery of antimicrobials for pathogens incapable of acquiring sufficient riboflavin from their hosts. The homodimer of 23 kDa subunits requires Mg{sup 2+} for activity. The first three-dimensional structure of the enzyme was determined at 1.4 {angstrom} resolution using the multiwavelength anomalous diffraction (MAD) method on Escherichia coli protein crystals containing gold. The protein consists of an {alpha} + {beta} fold having a complex linkage of {beta} strands. Intersubunit contacts are mediated by numerous hydrophobic interactions and three hydrogen bond networks. A proposed active site was identified on the basis of amino acid residues that are conserved among the enzyme from 19 species. There are two well-separated active sites per dimer, each of which comprise residues from both subunits. In addition to three arginines and two threonines, which may be used for recognizing the phosphate group of the substrate, the active site consists of three glutamates, two aspartates, two histidines, and a cysteine which may provide the means for general acid and base catalysis and for coordinating the Mg{sup 2+} cofactor within the active site.

  8. 11B Cross Section

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    p, X) (Current as of 12/17/2015) NSR Reaction Ep (MeV) Cross Section File X4 Dataset Date Added 2004RO27, 2004SP03 11B(p, α): deduced σ, S-factor Ecm ~ 0 - 1 X4 11/07/2012 2010LA11 11B(p, α): deduced S-factor E(cm) = 0 - 0.6 1 11/30/2011 2000KE10 11B(pol. p, γ): σ, deduced S-factor < 100 keV X4 11/07/2012 1993AN06 11B(p, α): α yield E(cm) = 17 - 134 keV X4 11/29/2012 1979DA03 11B(p, 3α): σ 35.4 - 1500 keV X4 07/30/2014 1992CE02 11B(p, γ): deduced S-factor 40 - 180 keV X4 03/07/2012

  9. Ca(OH)[sub 2]/fly ash sorbents for SO[sub 2] removal

    SciTech Connect (OSTI)

    Ho, C.S.; Shih, S.M. )

    1992-04-01

    In this paper, the reactivity of Ca(OH)[sub 2]/fly ash sorbent with SO[sub 2] is studied by using a fixed-bed differential reactor under the conditions simulating the bag filters of the spray-drying flue gas desulfurization. The source of fly ash and the sorbent preparation conditions affect the reactivity of the sorbent. The reactivity of the sorbent was found to be closely related to the content of the calcium silicate hydrate formed in the sorbent preparation. The sorbent has a much higher utilization of Ca(OH)[sub 2] than that of pure Ca(OH)[sub 2] sorbent, and in some range of Ca(OH)[sub 2] content the sorbent also has a higher SO[sub 2] capture capacity per unit weight of sorbent than that of pure lime. The fly ash from the Shin-Da plant of the Taiwan Power Company produced the best sorbent of all fly ashes in this study. The higher ratio of fly ash/Ca(OH)[sub 2], the higher slurrying temperature, the longer slurrying time, and the smaller particles of fly ash enhance the utilization of Ca(OH)[sub 2], but the water/solid ratio has an optimal value. The relative humidity in the reactor has a significant effect on the reactivity of Ca(PH)[sub 2]/fly ash sorbents, but the effect of the sulfation temperature is subtle.

  10. Response of a water-filled spherical vessel to an internal explosion

    SciTech Connect (OSTI)

    Lewis, M.W.; Wilson, T.L.

    1997-06-01

    Many problems of interest to the defense community involve fluid-structure interaction (FSI). Such problems include underwater blast loading of structures, bubble dynamics and jetting around structures, and hydrodynamic ram events. These problems may involve gas, fluid, and solid dynamics, nonlinear material behavior, cavitation, reaction kinetics, material failure, and nonlinearity that is due to varying geometry and contact conditions within a structure or between structures. Here, the authors model the response of a water-filled, thick-walled, spherical steel vessel to an internal explosion of 30 grams of C-4 with FSI2D--a two-dimensional coupled finite element and finite volume hydrodynamics code. The gas phase detonation products were modeled with a Becker-Kistiakowsky-Wilson high-explosive equation of state. Predictions from a fully coupled model were compared to experimental results in the form of strain gauge traces. Agreement was reasonably good. Additionally, the calculation was run in an uncoupled mode to understand the importance of fluid-structure interaction in this problem. The uncoupled model results in an accumulation of nonphysical energy in the vessel.

  11. Crystallization and preliminary X-ray analysis of a class II release factor RF3 from a sulfate-reducing bacterium

    SciTech Connect (OSTI)

    Kihira, Kiyohito; Numata, Shuko; Kitamura, Masaya; Kondo, Jun; Terawaki, Shinichi; Shomura, Yasuhito; Komori, Hirofumi; Shibata, Naoki; Higuchi, Yoshiki

    2008-07-01

    Class II release factor 3 (RF3) from the sulfate-reducing bacterium D. vulgaris Miyazaki F has been overexpressed, purified and crystallized in complex with GDP. Class II release factor 3 (RF3) from the sulfate-reducing bacterium Desulfovibrio vulgaris Miyazaki F, which promotes rapid dissociation of a class I release factor, has been overexpressed, purified and crystallized in complex with GDP at 293 K using the sitting-drop vapour-diffusion method. A data set was collected to 1.8 resolution from a single crystal at 100 K using synchrotron radiation. The crystal belongs to space group P1, with unit-cell parameters a = 47.39, b = 82.80, c = 148.29 , ? = 104.21, ? = 89.78, ? = 89.63. The asymmetric unit contains four molecules of the RF3GDP complex. The Matthews coefficient was calculated to be 2.3 {sup 3} Da{sup ?1} and the solvent content was estimated to be 46.6%.

  12. Crystallization and preliminary X-ray analysis of AbsC, a novel regulator of antibiotic production in Streptomyces coelicolor

    SciTech Connect (OSTI)

    Stevenson, Clare E. M.; Kock, Holger; Mootien, Saraspadee; Davies, San C.; Bibb, Mervyn J.; Lawson, David M.

    2007-03-01

    A novel regulator of antibiotic production in S. coelicolor, AbsC, has been crystallized in space group P2{sub 1}2{sub 1}2{sub 1}. X-ray data to 2.25 resolution were collected on station PX 14.1 at Daresbury. Crystals of recombinant AbsC (subunit MW = 18 313 Da; 158 amino acids), a novel regulator of antibiotic production from Streptomyces coelicolor, were grown by vapour diffusion. The protein crystallizes in space group P2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 43.53, b = 121.30, c = 143.75 . Native data to a resolution of 2.25 were recorded at station PX 14.1 (Daresbury) from a single crystal. Preliminary analysis of these data suggests that the asymmetric unit contains four copies of the AbsC monomer, giving an estimated solvent content of 47.0%. AbsC belongs to the MarR family of proteins that mediate ligand-responsive transcriptional control.

  13. Structural and functional characterization of the enantiomers of the antischistosomal drug oxamniquine

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Taylor, Alexander B.; Pica-Mattoccia, Livia; Polcaro, Chiara M.; Donati, Enrica; Cao, Xiaohang; Basso, Annalisa; Guidi, Alessandra; Rugel, Anastasia R.; Holloway, Stephen P.; Anderson, Timothy J.C.; et al

    2015-10-20

    For over two decades, a racemic mixture of oxamniquine (OXA) was administered to patients infected by Schistosoma mansoni, but whether one or both enantiomers exert antischistosomal activity was unknown. Recently, a ~30 kDa S. mansoni sulfotransferase (SmSULT) was identified as the target of OXA action. Here, we separate the OXA enantiomers using chromatographic methods and assign their optical activities as dextrorotary [(+)-OXA] or levorotary [(-)-OXA]. Crystal structures of the parasite enzyme in complex with optically pure (+)-OXA and (-)-OXA) reveal their absolute configurations as S- and R-, respectively. When tested in vitro, S-OXA demonstrated the bulk of schistosomicidal activity, whilemore » R-OXA had antischistosomal effects when present at relatively high concentrations. Crystal structures R-OXA•SmSULT and S-OXA•SmSULT complexes reveal similarities in the modes of OXA binding, but only the S-OXA enantiomer is observed in the structure of the enzyme exposed to racemic OXA. Together the data suggest the higher schistosomicidal activity of S-OXA is correlated with its ability to outcompete R-OXA binding the sulfotransferase active site. In conclusion, these findings have important implications for the design, syntheses, and dosing of new OXA-based antischistosomal compounds.« less

  14. The interaction of high-speed turbulence with flames: Global properties and internal flame structure

    SciTech Connect (OSTI)

    Poludnenko, A.Y.; Oran, E.S. [Laboratory for Computational Physics and Fluid Dynamics, Naval Research Laboratory, Washington, DC 20375 (United States)

    2010-05-15

    We study the dynamics and properties of a turbulent flame, formed in the presence of subsonic, high-speed, homogeneous, isotropic Kolmogorov-type turbulence in an unconfined system. Direct numerical simulations are performed with Athena-RFX, a massively parallel, fully compressible, high-order, dimensionally unsplit, reactive flow code. A simplified reaction-diffusion model represents a stoichiometric H{sub 2}-air mixture. The system being modeled represents turbulent combustion with the Damkoehler number Da=0.05 and with the turbulent velocity at the energy injection scale 30 times larger than the laminar flame speed. The simulations show that flame interaction with high-speed turbulence forms a steadily propagating turbulent flame with a flame brush width approximately twice the energy injection scale and a speed four times the laminar flame speed. A method for reconstructing the internal flame structure is described and used to show that the turbulent flame consists of tightly folded flamelets. The reaction zone structure of these is virtually identical to that of the planar laminar flame, while the preheat zone is broadened by approximately a factor of two. Consequently, the system evolution represents turbulent combustion in the thin reaction zone regime. The turbulent cascade fails to penetrate the internal flame structure, and thus the action of small-scale turbulence is suppressed throughout most of the flame. Finally, our results suggest that for stoichiometric H{sub 2}-air mixtures, any substantial flame broadening by the action of turbulence cannot be expected in all subsonic regimes. (author)

  15. Methylocystis strain SB2 materials and methods

    DOE Patents [OSTI]

    Semrau, Jeremy D; Gallagher, Warren; Yoon, Sukhwan; Im, Jeongdae; DiSpririto, Alan A; Lee, Sung-Woo; Hartsel, Scott; McEllistrem, Marcus T

    2014-01-14

    The present disclosures provides isolated or purified compounds, each of which bind to a metal atom. Generally, the compounds are small in size (e.g., molecular weight of less than about 1 kDa) and peptidic in nature, inasmuch as the compounds comprise amino acids. In some embodiments, the compound comprises a structure of Formula I; M.sub.1-P.sub.1-M.sub.2-P.sub.2 wherein each of P.sub.1 and P.sub.2 is a peptide comprising at least two amino acids, M.sub.1 is a first metal binding moiety comprising a substituted imidazolone ring, M.sub.2 is a second metal binding moiety comprising a substituted oxazolone ring, and wherein M.sub.1 and M.sub.2 bind to a single metal atom. Also provided are related complexes, conjugates, cells which synthesize the compounds of the present disclosures, substantially homogenous cultures thereof, kits and compositions, and methods of making or using the materials of the present disclosures.

  16. Super-B Project Overview

    SciTech Connect (OSTI)

    Biagini, M.E.; Boni, R.; Boscolo, M.; Demma, T.; Drago, A.; Guiducci, S.; Raimondi, P.; Tomassini, S.; Zobov, M.; Bertsche, K.; Donald, M.; Nosochkov, Y.; Novokhatski, A.; Seeman, J.; Sullivan, M.; Yocky, G.; Wienands, U.; Wittmer, W.; Koop, I.; Levichev, E.; Nikitin, S.; /Novosibirsk, IYF /KEK, Tsukuba /Pisa U. /CERN

    2010-08-26

    The SuperB project aims at the construction of an asymmetric very high luminosity B-Factory on the Frascati/Tor Vergata (Italy) area, providing a uniquely sensitive probe of New Physics in the flavour sector of the Standard Model. The luminosity goal of 10{sup 36} cm{sup -2} s{sup -1} can be reached with a new collision scheme with 'large Piwinski angle' (LPA) and the use of 'crab waist sextupoles' (CW). A LPA&CW Interaction Region (IR) has been successfully tested at the DA{Phi}NE {Phi}-Factory at LNF-Frascati in 2008. The LPA&CW scheme, together with very low {beta}*, will allow for operation with relatively low beam currents and reasonable bunch length, comparable to those of PEP-II and KEKB. In the High Energy Ring (HER), two spin rotators will bring longitudinally polarized beams into collision at the IP. The lattice has been designed with a very low intrinsic emittance and is quite compact, less than 2 km long. The tight focusing requires the final doublet quadrupoles to be very close to the IP and very compact. A Conceptual Design Report was published in March 2007, and beam dynamics and collective effects R&D studies are in progress in order to publish a Technical Design Report by the end of 2010.

  17. Spent sealed radium sources conditioning in Latin America

    SciTech Connect (OSTI)

    Mourao, R.P.

    1999-06-01

    The management of spent sealed sources is considered by the International Atomic Energy Agency (IAEA) one of the greatest challenges faced by nuclear authorities today, especially in developing countries. One of the Agency`s initiatives to tackle this problem is the Spent Radium Sources Conditioning Project, a worldwide project relying on the regional co-operation between countries. A team from the Brazilian nuclear research institute Centro de Desenvolvimento da Tecnologia Nuclear (CDTN) was chosen as the expert team to carry out the operations in Latin America; since December 1996 radium sources have been safely conditioned in Uruguay, Nicaragua, Guatemala, Ecuador and Paraguay. A Quality Assurance Program was established, encompassing the qualification of the capsule welding process, written operational procedures referring to all major steps of the operation, calibration of monitors and information retrievability. A 200L carbon steel drum-based packaging concept was used to condition the sources, its cavity being designed to receive the lead shield device containing stainless steel capsules with the radium sources. As a result of these operations, a total amount of 2,897 mg of needles, tubes, medical applicators, standard sources for calibration, lightning rods, secondary wastes and contaminated objects were stored in proper conditions and are now under control of the nuclear authorities of the visited countries.

  18. Inhibition of Alkaline Flocculation by Algal Organic Matter for Chlorella vulgaris

    SciTech Connect (OSTI)

    Vandamme, Dries; Beuckels, Annelies; Vadelius, Eric; Depraetere, Orily; Noppe, Wim; Dutta, Abhishek; Foubert, Imogen; Laurens, Lieve; Muylaert, Koenraad

    2016-01-01

    Alkaline flocculation is a promising strategy for the concentration of microalgae for bulk biomass production. However, previous studies have shown that biological changes during the cultivation negatively affect flocculation efficiency. The influence of changes in cell properties and in the quality and composition of algal organic matter (AOM) were studied using Chlorella vulgaris as a model species. In batch cultivation, flocculation was increasingly inhibited over time and mainly influenced by changes in medium composition, rather than biological changes at the cell surface. Total carbohydrate content of the organic matter fraction sized bigger than 3 kDa increased over time and this fraction was shown to be mainly responsible for the inhibition of alkaline flocculation. The monosaccharide identification of this fraction mainly showed the presence of neutral and anionic monosaccharides. An addition of 30–50 mg L-1 alginic acid, as a model for anionic carbohydrate polymers containing uronic acids, resulted in a complete inhibition of flocculation. Furthermore, these results suggest that inhibition of alkaline flocculation was caused by interaction of anionic polysaccharides leading to an increased flocculant demand over time.

  19. Distributed computing strategies for processing of FT-ICR MS imaging datasets for continuous mode data visualization

    SciTech Connect (OSTI)

    Smith, Donald F.; Schulz, Carl; Konijnenburg, Marco; Kilic, Mehmet; Heeren, Ronald M.

    2015-03-01

    High-resolution Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry imaging enables the spatial mapping and identification of biomolecules from complex surfaces. The need for long time-domain transients, and thus large raw file sizes, results in a large amount of raw data (“big data”) that must be processed efficiently and rapidly. This can be compounded by largearea imaging and/or high spatial resolution imaging. For FT-ICR, data processing and data reduction must not compromise the high mass resolution afforded by the mass spectrometer. The continuous mode “Mosaic Datacube” approach allows high mass resolution visualization (0.001 Da) of mass spectrometry imaging data, but requires additional processing as compared to featurebased processing. We describe the use of distributed computing for processing of FT-ICR MS imaging datasets with generation of continuous mode Mosaic Datacubes for high mass resolution visualization. An eight-fold improvement in processing time is demonstrated using a Dutch nationally available cloud service.

  20. The Status of USITER Diagnostic Port Plug Neutronics Analysis Using Attila

    SciTech Connect (OSTI)

    Feder, Russell; Youssef, Mahamoud; Klabacha, Jonathan

    2013-11-01

    USITER is one of seven partner domestic agencies (DA) contributing components to the ITER project. Four diagnostic port plug packages (two equatorial ports and two upper ports) will be engineered and fabricated by Princeton Plasma Physics Lab (PPPL). Diagnostic port plugs as illustrated in Fig. 1 are large primarily stainless steel structures that serve several roles on ITER. The port plugs are the primary vacuum seal and tritium confinement barriers for the vessel. The port plugs also house several plasma diagnostic systems and other machine service equipment. Finally, each port plug must shield high energy neutrons and gamma photons from escaping and creating radiological problems in maintenance areas behind the port plugs. The optimization of the balance between adequate shielding and the need for high performance, high throughput diagnostics systems is the focus of this paper. Neutronics calculations are also needed for assessing nuclear heating and nuclear damage in the port plug and diagnostic components. Attila, the commercially available discrete-ordinates software package, is used for all diagnostic port plug neutronics analysis studies at PPPL.

  1. Structure of a bacterial virus DNA-injection protein complex reveals a decameric assembly with a constricted molecular channel

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Zhao, Haiyan; Speir, Jeffrey A.; Matsui, Tsutomu; Lin, Zihan; Liang, Lingfei; Lynn, Anna Y.; Varnado, Brittany; Weiss, Thomas M.; Tang, Liang; Schuch, Raymond

    2016-02-16

    The multi-layered cell envelope structure of Gram-negative bacteria represents significant physical and chemical barriers for short-tailed phages to inject phage DNA into the host cytoplasm. Here we show that a DNA-injection protein of bacteriophage Sf6, gp12, forms a 465-kDa, decameric assembly in vitro. The electron microscopic structure of the gp12 assembly shows a ~150-Å, mushroom-like architecture consisting of a crown domain and a tube-like domain, which embraces a 25-Å-wide channel that could precisely accommodate dsDNA. The constricted channel suggests that gp12 mediates rapid, uni-directional injection of phage DNA into host cells by providing a molecular conduit for DNA translocation. Themore » assembly exhibits a 10-fold symmetry, which may be a common feature among DNA-injection proteins of P22-like phages and may suggest a symmetry mismatch with respect to the 6-fold symmetric phage tail. As a result, the gp12 monomer is highly flexible in solution, supporting a mechanism for translocation of the protein through the conduit of the phage tail toward the host cell envelope, where it assembles into a DNA-injection device.« less

  2. Integrated Approach to Reconstruction of Microbial Regulatory Networks

    SciTech Connect (OSTI)

    Rodionov, Dmitry A; Novichkov, Pavel S

    2013-11-04

    This project had the goal(s) of development of integrated bioinformatics platform for genome-scale inference and visualization of transcriptional regulatory networks (TRNs) in bacterial genomes. The work was done in Sanford-Burnham Medical Research Institute (SBMRI, P.I. D.A. Rodionov) and Lawrence Berkeley National Laboratory (LBNL, co-P.I. P.S. Novichkov). The developed computational resources include: (1) RegPredict web-platform for TRN inference and regulon reconstruction in microbial genomes, and (2) RegPrecise database for collection, visualization and comparative analysis of transcriptional regulons reconstructed by comparative genomics. These analytical resources were selected as key components in the DOE Systems Biology KnowledgeBase (SBKB). The high-quality data accumulated in RegPrecise will provide essential datasets of reference regulons in diverse microbes to enable automatic reconstruction of draft TRNs in newly sequenced genomes. We outline our progress toward the three aims of this grant proposal, which were: Develop integrated platform for genome-scale regulon reconstruction; Infer regulatory annotations in several groups of bacteria and building of reference collections of microbial regulons; and Develop KnowledgeBase on microbial transcriptional regulation.

  3. Crystallization and Preliminary Diffraction Analysis of the CAL PDZ Domain in Complex with a Selective Peptide Inhibitor

    SciTech Connect (OSTI)

    J Amacher; P Cushing; J Weiner; D Madden

    2011-12-31

    Cystic fibrosis (CF) is associated with loss-of-function mutations in the CF transmembrane conductance regulator (CFTR), which regulates epithelial fluid and ion homeostasis. The CFTR cytoplasmic C-terminus interacts with a number of PDZ (PSD-95/Dlg/ZO-1) proteins that modulate its intracellular trafficking and chloride-channel activity. Among these, the CFTR-associated ligand (CAL) has a negative effect on apical-membrane expression levels of the most common disease-associated mutant {Delta}F508-CFTR, making CAL a candidate target for the treatment of CF. A selective peptide inhibitor of the CAL PDZ domain (iCAL36) has recently been developed and shown to stabilize apical expression of {Delta}F508-CFTR, enhancing net chloride-channel activity, both alone and in combination with the folding corrector corr-4a. As a basis for structural studies of the CAL-iCAL36 interaction, a purification protocol has been developed that increases the oligomeric homogeneity of the protein. Here, the cocrystallization of the complex in space group P2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 35.9, b = 47.7, c = 97.3 {angstrom}, is reported. The crystals diffracted to 1.4 {angstrom} resolution. Based on the calculated Matthews coefficient (1.96 {angstrom}{sup 3} Da{sup -1}), it appears that the asymmetric unit contains two complexes.

  4. Purification, crystallization and preliminary X-ray diffraction analysis of the kinase domain of human tousled-like kinase 2

    SciTech Connect (OSTI)

    Garrote, Ana M.; Redondo, Pilar; Montoya, Guillermo; Muoz, Ins G.

    2014-02-19

    The C-terminal kinase domain of TLK2 (a human tousled-like kinase) has been cloned and overexpressed in Escherichia coli followed by purification to homogeneity. Crystallization experiments in the presence of ATP-?-S yielded crystals suitable for X-ray diffraction analysis belonging to two different space groups: tetragonal I4{sub 1}22 and cubic P2{sub 1}3. Tousled-like kinases (TLKs) are an evolutionarily conserved family of serine/threonine protein kinases involved in chromatin dynamics, including DNA replication and repair, transcription and chromosome segregation. The two members of the family reported in humans, namely TLK1 and TLK2, localize to the cell nucleus and are capable of forming homo- or hetero-oligomers by themselves. To characterize the role of TLK2, its C-terminal kinase domain was cloned and overexpressed in Escherichia coli followed by purification to homogeneity. Crystallization experiments in the presence of ATP-?-S yielded crystals suitable for X-ray diffraction analysis belonging to two different space groups: tetragonal I4{sub 1}22 and cubic P2{sub 1}3. The latter produced the best diffracting crystal (3.4 resolution using synchrotron radiation), with unit-cell parameters a = b = c = 126.05 , ? = ? = ? = 90. The asymmetric unit contained one protein molecule, with a Matthews coefficient of 4.59 {sup 3} Da{sup ?1} and a solvent content of 73.23%.

  5. The RACER (risk analysis, communication, evaluation, and reduction) stakeholder environmental data transparency project for Los Alamos National Laboratory

    SciTech Connect (OSTI)

    Echohawk, John Chris; Dorries, Alison M; Eberhart, Craig F; Werdel, Nancy

    2008-01-01

    The RACER (Risk Analysis, Communication, Evaluation, and Reduction) project was created in 2003, as an effort to enhance the Los Alamos National Laboratory's ability to effectively communicate the data and processes used to evaluate environmental risks to the public and the environment. The RACER project staff consists of members of Risk Assessment Corporation, Los Alamos National Laboratory (LANL), and the New Mexico Environment Department (NMED). RACER staff worked closely with members of the community, tribal governments, and others within NMED and LANL to create innovative tools and a process that could provide information to regulators, LANL and the community about the sources of public health risk and ecological impact from LAN L operations. The RACER Data Analysis Tool (DA T) provides the public with webbased access to environmental measurement data collected in and around the LANL site. Its purpose is to provide a 'transparent' view to the public of all data collected by LANL and NMED regarding the LANL site. The DAT is available to the public at 'www.racernm.com'.

  6. Super-Stable, Highly Monodisperse Plasmonic Nanocrystals with 500 Gold Atoms: Au~500(SR)~120

    SciTech Connect (OSTI)

    Kumara, Chanaka; Zuo, Xiaobing; Ilavsky, Dr. Jan; Chapman, Karena; Cullen, David A; Dass, Amala

    2014-01-01

    Determining the composition of plasmonic nanoparticles is challenging due to a deficiency in tools capable of accurately evaluating the number of atoms. Mass spectrometry plays a significant role in determining nanoparticle composition at the atomic level. Significant progress has been made in understanding ultra-small gold nanoparticles, like Au25(SR)18 and Au38(SR)24, with a Au core diameter of 0.97 and 1.3 nm, respectively. However, progress in small plasmonic nanoparticles (2 - 5 nm) is currently challenging, due in part to limitations in synthesizing monodisperse nanoparticles. Here, we report a plasmonic nanocrystal that is highly monodisperse, with an unprecedented variation of less than 20 gold atoms. The composition of the super-stable plasmonic nanocrystals at 115 kDa was determined to contain Au500 10SR120 3. The Au~500 system, named Faraduarate-500, is the largest size to be characterized using high resolution ESI mass spectrometry. Atomic pair distribution function (PDF) data shows that the local atomic structure is consistent with a face-centered cubic (fcc) or Marks decahedral arrangement. High resolution scanning transmission electron microscopy images show that the diameter is 2.4 0.1 nm. The radius of gyration measured by small angle X-ray scattering (SAXS), is 1.05 0.05 nm, and the size and the shape of SAXS molecular envelope are in agreement with TEM and PDF measurements.

  7. GASEOUS HYDROGEN EFFECTS ON THE MECHANICAL PROPERTIES OF CARBON AND LOW ALLOY STEELS (U)

    SciTech Connect (OSTI)

    Lam, P

    2006-06-08

    This report is a compendium of sets of mechanical properties of carbon and low alloy steels following the short-term effects of hydrogen exposure. The property sets include the following: Yield Strength; Ultimate Tensile Strength; Uniform Elongation; Reduction of Area; Threshold Cracking, K{sub H} or K{sub th}; Fracture Toughness (K{sub IC}, J{sub IC}, and/or J-R Curve); and Fatigue Crack Growth (da/dN). These properties are drawn from literature sources under a variety of test methods and conditions. However, the collection of literature data is by no means complete, but the diversity of data and dependency of results in test method is sufficient to warrant a design and implementation of a thorough test program. The program would be needed to enable a defensible demonstration of structural integrity of a pressurized hydrogen system. It is essential that the environmental variables be well-defined (e.g., the applicable hydrogen gas pressure range and the test strain rate) and the specimen preparation be realistically consistent (such as the techniques to charge hydrogen and to maintain the hydrogen concentration in the specimens).

  8. Metabonomic Profiling of TASTPM Transgenic Alzheimer's Disease Mouse Model

    SciTech Connect (OSTI)

    Hu, Zeping; Browne, Edward R.; Liu, Tao; Angel, Thomas E.; Ho, Paul C.; Chun Yong Chan, Eric

    2012-12-07

    Identification of molecular mechanisms underlying early stage Alzheimer’s disease (AD) is important for the development of new therapies against and diagnosis of AD. In this study, non-targeted metabotyping of TASTPM transgenic AD mice was performed. The metabolic profiles of both brain and plasma of TASTPM mice were characterized using gas chromatography-mass spectrometry and compared to those of wild type C57BL/6J mice. TASTPM mice were metabolically distinct compared to wild type mice (Q28 Y = 0.587 and 0.766 for PLS-DA models derived from brain and plasma, respectively). A number of metabolites were found to be perturbed in TASTPM mice in both brain (D11 fructose, L-valine, L-serine, L-threonine, zymosterol) and plasma (D-glucose, D12 galactose, linoleic acid, arachidonic acid, palmitic acid and D-gluconic acid). In addition, enzyme immunoassay confirmed that selected endogenous steroids were significantly perturbed in brain (androstenedione and 17-OH-progesterone) and plasma (cortisol and testosterone) of TASTPM mice. Ingenuity pathway analysis revealed that perturbations related to amino acid metabolism (brain), steroid biosynthesis (brain), linoleic acid metabolism (plasma) and energy metabolism (plasma) accounted for the differentiation of TASTPM and wild-type

  9. Pyrolysis of tire rubber: Porosity and adsorption characteristics of the pyrolytic chars

    SciTech Connect (OSTI)

    Miguel, G.S.; Fowler, G.D.; Sollars, C.J.

    1998-06-01

    Tire rubber has been pyrolyzed at various temperatures under a nitrogen atmosphere. The resulting chars have been analyzed for their porosity using nitrogen gas adsorption and for their aqueous adsorption characteristics using phenol, methylene blue, and the reactive dyes Procion Turquoise H-A and Procion Red H-E3B. Nitrogen adsorption isotherms were modeled to the BET and Dubinin-Astakhov (DA) equations to determine effective surface areas, mesopore volumes, and micropore volumes. Results showed that pyrolysis of tire rubber was essentially complete at 500 C and resulted in a char yield of approximately 42 wt%. Pyrolytic chars exhibited BET surface areas up to 85 m{sup 2}/g and micropore volumes up to 0.04 mL/g. Owing to their poorly developed micropore structure, the pyrolytic chars exhibited limited aqueous adsorption capacity for compounds of small molecular weight, such as phenol. However, the chars possessed significantly greater adsorption capacity for species of large molecular weight which was attributed to the presence of large mesopore volumes (up to 0.19 mL/g).

  10. The production, purification and crystallization of a soluble form of the nonclassical MHC HLA-G: the essential role of cobalt

    SciTech Connect (OSTI)

    Clements, Craig S.; Kjer-Nielsen, Lars; Kostenko, Lyudmila; McCluskey, James; Rossjohn, Jamie

    2006-01-01

    X-ray diffraction data were collected to 1.9 from crystals of HLA-G. Cobalt ions were found to be essential for the production of diffracting crystals. HLA-G is a nonclassical class I major histocompatibility complex (MHC) molecule that is primarily expressed at the foetalmaternal interface. Although the role of HLA-G has not been fully elucidated, current evidence suggests it protects the foetus from the maternal immune response. In this report, HLA-G (44 kDa) is characterized by expression in Escherichia coli. The inclusion bodies were refolded in complex with a peptide derived from histone H2A (RIIPRHLQL), purified and subsequently crystallized. Correct refolding was determined using two conformation-dependent antibodies. Cobalt ions were shown to be an essential ingredient for obtaining diffraction-quality crystals. The crystals, which diffracted to 1.9 resolution, belonged to space group P3{sub 2}2{sub 1}, with unit-cell parameters a = b = 77.15, c = 151.72 .

  11. Summary Report of Cable Aging and Performance Data for Fiscal Year 2014.

    SciTech Connect (OSTI)

    Celina, Mathias C.; Celina, Mathias C.; Redline, Erica Marie; Redline, Erica Marie; Bernstein, Robert; Bernstein, Robert; Quintana, Adam; Quintana, Adam; Giron, Nicholas Henry; Giron, Nicholas Henry; White II, Gregory Von; White II, Gregory Von

    2014-09-01

    As part of the Light Water Reactor Sustainability Program, science - based engineering approaches were employed to address cable degradation behavior under a range of exposure environments. Experiments were conducted with the goal to provide best guidance for aged material states, remaining life and expected performance under specific conditions for a range of cable materials. Generic engineering tests , which focus on rapid accelerated aging and tensile elongation , were combined with complementar y methods from polymer degradation science. Sandia's approach, building on previous years' efforts, enabled the generation of some of the necessary data supporting the development of improved lifetime predictions models, which incorporate known material b ehaviors and feedback from field - returned 'aged' cable materials. Oxidation rate measurements have provided access to material behavior under low dose rate thermal conditions, where slow degradation is not apparent in mechanical property changes. Such da ta have shown aging kinetics consistent with established radiati on - thermal degradation models. ACKNOWLEDGEMENTS We gratefully acknowledge ongoing technical support at the LICA facility and extensive sample handling provided by Maryla Wasiolek and Don Hans on. Sam Durbin and Patrick Mattie are recognized for valuable guidance throughout the year and assistance in the preparation of the final report. Doug Brunson is appreciated for sample analysis, compilation and plotting of experimental data.

  12. Purification, crystallization and preliminary X-ray diffraction studies to near-atomic resolution of dihydrodipicolinate synthase from methicillin-resistant Staphylococcus aureus

    SciTech Connect (OSTI)

    Burgess, Benjamin R.; Dobson, Renwick C. J. Dogovski, Con; Jameson, Geoffrey B.; Parker, Michael W.; Perugini, Matthew A.

    2008-07-01

    Dihydrodipicolinate synthase (DHDPS), an enzyme of the lysine-biosynthetic pathway, is a promising target for antibiotic development against pathogenic bacteria. Here, the expression, purification, crystallization and preliminary diffraction analysis to 1.45 Å resolution of DHDPS from methicillin-resistant S. aureus is reported. In recent years, dihydrodipicolinate synthase (DHDPS; EC 4.2.1.52) has received considerable attention from both mechanistic and structural viewpoints. DHDPS is part of the diaminopimelate pathway leading to lysine, coupling (S)-aspartate-β-semialdehyde with pyruvate via a Schiff base to a conserved active-site lysine. In this paper, the cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of DHDPS from methicillin-resistant Staphylococcus aureus, an important bacterial pathogen, are reported. The enzyme was crystallized in a number of forms, predominantly from PEG precipitants, with the best crystal diffracting to beyond 1.45 Å resolution. The space group was P1 and the unit-cell parameters were a = 65.4, b = 67.6, c = 78.0 Å, α = 90.1, β = 68.9, γ = 72.3°. The crystal volume per protein weight (V{sub M}) was 2.34 Å{sup 3} Da{sup −1}, with an estimated solvent content of 47% for four monomers per asymmetric unit. The structure of the enzyme will help to guide the design of novel therapeutics against the methicillin-resistant S. aureus pathogen.

  13. Gas centrifuge enrichment plants inspection frequency and remote monitoring issues for advanced safeguards implementation

    SciTech Connect (OSTI)

    Boyer, Brian David; Erpenbeck, Heather H; Miller, Karen A; Ianakiev, Kiril D; Reimold, Benjamin A; Ward, Steven L; Howell, John

    2010-09-13

    Current safeguards approaches used by the IAEA at gas centrifuge enrichment plants (GCEPs) need enhancement in order to verify declared low enriched uranium (LEU) production, detect undeclared LEU production and detect high enriched uranium (BEU) production with adequate probability using non destructive assay (NDA) techniques. At present inspectors use attended systems, systems needing the presence of an inspector for operation, during inspections to verify the mass and {sup 235}U enrichment of declared cylinders of uranium hexafluoride that are used in the process of enrichment at GCEPs. This paper contains an analysis of how possible improvements in unattended and attended NDA systems including process monitoring and possible on-site destructive analysis (DA) of samples could reduce the uncertainty of the inspector's measurements providing more effective and efficient IAEA GCEPs safeguards. We have also studied a few advanced safeguards systems that could be assembled for unattended operation and the level of performance needed from these systems to provide more effective safeguards. The analysis also considers how short notice random inspections, unannounced inspections (UIs), and the concept of information-driven inspections can affect probability of detection of the diversion of nuclear material when coupled to new GCEPs safeguards regimes augmented with unattended systems. We also explore the effects of system failures and operator tampering on meeting safeguards goals for quantity and timeliness and the measures needed to recover from such failures and anomalies.

  14. Feasibility studies to establish at the Kazakhstan Ulba metallurgical plant the manufacturing capability to produce low-enriched uranium certified reference materials

    SciTech Connect (OSTI)

    Kuzminski, Jozef; Nesuhoff, J; Cratto, P; Pfennigwerth, G; Mikhailenko, A; Maliutina, I; Nations, J

    2009-01-01

    One of the salient features of the transition plan that the United States Department of Energy/National Nuclear Security Administration (DOE/NNSA) is presently implementing in the Former Soviet Union countries is the availability of uranium certified reference materials for calibration of nondestructive assay (NDA) measurement equipment. To address this challenge, DOE/NNSA and U.S. national laboratories have focused their cooperative efforts on establishing a reliable source for manufacturing, certifying, and supplying of such standards. The Ulba Metallurgical Plant (UMP), Kazakhstan, which processes large quantities of low-enriched uranium to produce ceramic fuel pellets for nuclear-powered reactors, is well situated to become a key supplier of low-enriched uranium certified reference materials for the country and Central Asia region. We have recently completed Phase I of a feasibility study to establish at UMP capabilities of manufacturing these standards. In this paper we will discuss details of a proposed methodology for uranium down-blending, material selection and characterization, and a proposed methodology of measurement by destructive (DA) and non-destructive (NDA) analysis to form a database for material certification by the competent State authorities in the Republic of Kazakhstan. In addition, we will discuss the prospect for manufacturing of such standards at UMP.

  15. Analysis of the effectiveness of gas centrifuge enrichment plants advanced safeguards

    SciTech Connect (OSTI)

    Boyer, Brian David; Erpenbeck, Heather H; Miller, Karen A; Swinjoe, Martyn T; Ianakiev, Kiril D; Marlow, Johnna B

    2010-01-01

    Current safeguards approaches used by the International Atomic Energy Agency (IAEA) at gas centrifuge enrichment plants (GCEPs) need enhancement in order to verify declared low-enriched uranium (LEU) production, detect undeclared LEU production and detect highly enriched uranium (HEU) production with adequate detection probability using non destructive assay (NDA) techniques. At present inspectors use attended systems, systems needing the presence of an inspector for operation, during inspections to verify the mass and 235U enrichment of declared UF6 containers used in the process of enrichment at GCEPs. This paper contains an analysis of possible improvements in unattended and attended NDA systems including process monitoring and possible on-site destructive assay (DA) of samples that could reduce the uncertainty of the inspector's measurements. These improvements could reduce the difference between the operator's and inspector's measurements providing more effective and efficient IAEA GCEPs safeguards. We also explore how a few advanced safeguards systems could be assembled for unattended operation. The analysis will focus on how unannounced inspections (UIs), and the concept of information-driven inspections (IDS) can affect probability of detection of the diversion of nuclear materials when coupled to new GCEPs safeguards regimes augmented with unattended systems.

  16. Atomic force microscopy investigation of the giant mimivirus

    SciTech Connect (OSTI)

    Kuznetsov, Yuri G.; Xiao Chuan; Sun Siyang; Raoult, Didier; Rossmann, Michael; McPherson, Alexander

    2010-08-15

    Mimivirus was investigated by atomic force microscopy in its native state following serial degradation by lysozyme and bromelain. The 750-nm diameter virus is coated with a forest of glycosylated protein fibers of lengths about 140 nm with diameters 1.4 nm. Fibers are capped with distinctive ellipsoidal protein heads of estimated Mr = 25 kDa. The surface fibers are attached to the particle through a layer of protein covering the capsid, which is in turn composed of the major capsid protein (MCP). The latter is organized as an open network of hexagonal rings with central depressions separated by 14 nm. The virion exhibits an elaborate apparatus at a unique vertex, visible as a star shaped depression on native particles, but on defibered virions as five arms of 50 nm width and 250 nm length rising above the capsid by 20 nm. The apparatus is integrated into the capsid and not applied atop the icosahedral lattice. Prior to DNA release, the arms of the star disengage from the virion and it opens by folding back five adjacent triangular faces. A membrane sac containing the DNA emerges from the capsid in preparation for fusion with a membrane of the host cell. Also observed from disrupted virions were masses of distinctive fibers of diameter about 1 nm, and having a 7-nm periodicity. These are probably contained within the capsid along with the DNA bearing sac. The fibers were occasionally observed associated with toroidal protein clusters interpreted as processive enzymes modifying the fibers.

  17. HPV-18 E2circumflexE4 chimera: 2 new spliced transcripts and proteins induced by keratinocyte differentiation

    SciTech Connect (OSTI)

    Tan, Chye Ling; Gunaratne, Jayantha; Lai, Deborah; Carthagena, Laetitia; Wang, Qian; Xue, Yue Zhen; Quek, Ling Shih; Doorbar, John; Bachelerie, Francoise; Thierry, Francoise; Bellanger, Sophie

    2012-07-20

    The Human Papillomavirus (HPV) E4 is known to be synthesized as an E1circumflexE4 fusion resulting from splice donor and acceptor sites conserved across HPV types. Here we demonstrate the existence of 2 HPV-18 E2circumflexE4 transcripts resulting from 2 splice donor sites in the 5 Prime part of E2, while the splice acceptor site is the one used for E1circumflexE4. Both E2circumflexE4 transcripts are up-regulated by keratinocyte differentiation in vitro and can be detected in clinical samples containing low-grade HPV-18-positive cells from Pap smears. They give rise to two fusion proteins in vitro, E2circumflexE4-S and E2circumflexE4-L. Whereas we could not differentiate E2circumflexE4-S from E1circumflexE4 in vivo, E2circumflexE4-L could be formally identified as a 23 kDa protein in raft cultures in which the corresponding transcript was also found, and in a biopsy from a patient with cervical intraepithelial neoplasia stage I-II (CINI-II) associated with HPV-18, demonstrating the physiological relevance of E2circumflexE4 products.

  18. CENSUS OF BLUE STARS IN SDSS DR8

    SciTech Connect (OSTI)

    Scibelli, Samantha; Newberg, Heidi Jo; Carlin, Jeffrey L.; Yanny, Brian

    2015-01-01

    We present a census of the 12,060 spectra of blue objects ((g r){sub 0} < 0.25) in the Sloan Digital Sky Survey (SDSS) Data Release 8 (DR8). As part of the data release, all of the spectra were cross-correlated with 48 template spectra of stars, galaxies, and QSOs to determine the best match. We compared the blue spectra by eye to the templates assigned in SDSS DR8. 10,856 of the objects matched their assigned template, 170 could not be classified due to low signal-to-noise ratio, and 1034 were given new classifications. We identify 7458 DA white dwarfs, 1145 DB white dwarfs, 273 rarer white dwarfs (including carbon, DZ, DQ, and magnetic), 294 subdwarf O stars, 648 subdwarf B stars, 679 blue horizontal branch stars, 1026 blue stragglers, 13 cataclysmic variables, 129 white dwarf-M dwarf binaries, 36 objects with spectra similar to DO white dwarfs, 179, quasi-stellar objects (QSOs), and 10 galaxies. We provide two tables of these objects, sample spectra that match the templates, figures showing all of the spectra that were grouped by eye, and diagnostic plots that show the positions, colors, apparent magnitudes, proper motions, etc., for each classification. Future surveys will be able to use templates similar to stars in each of the classes we identify to automatically classify blue stars, including rare types.

  19. Sonochemical and hydrothermal synthesis of PbTe nanostructures with the aid of a novel capping agent

    SciTech Connect (OSTI)

    Fard-Fini, Shahla Ahmadian; Salavati-Niasari, Masoud; Mohandes, Fatemeh

    2013-10-15

    Graphical abstract: - Highlights: • PbTe nanostructures were prepared with the aid of Schiff-base compound. • Sonochemical and hydrothermal methods were employed to fabricate PbTe nanostrucrues. • The effect of preparation parameters on the morphology of PbTe was investigated. - Abstract: In this work, a new Schiff-base compound derived from 1,8-diamino-3,6-dioxaoctane and 2-hydroxy-1-naphthaldehyde marked as (2-HyNa)-(DaDo) was synthesized, characterized, and then used as capping agent for the preparation of PbTe nanostructures. To fabricate PbTe nanostructures, two different synthesis methods; hydrothermal and sonochemical routes, were applied. To further investigate, the effect of preparation parameters like reaction time and temperature in hydrothermal synthesis and sonication time in the presence of ultrasound irradiation on the morphology and purity of the final products was tested. The products were analyzed with the aid of SEM, TEM, XRD, FT-IR, and EDS. Based on the obtained results, it was found that pure cubic phased PbTe nanostructures have been obtained by hydrothermal and sonochemical approaches. Besides, SEM images showed that cubic-like and rod-like PbTe nanostructures have been formed by hydrothermal and sonochemical methods, respectively. Sonochemical synthesis of PbTe nanostructures was favorable, because the synthesis time of sonochemical method was shorter than that of hydrothermal method.

  20. Polyacrylamide medium for the electrophoretic separation of biomolecules

    DOE Patents [OSTI]

    Madabhushi, Ramakrishna S.; Gammon, Stuart A.

    2003-11-11

    A polyacryalmide medium for the electrophoretic separation of biomolecules. The polyacryalmide medium comprises high molecular weight polyacrylamides (PAAm) having a viscosity average molecular weight (M.sub.v) of about 675-725 kDa were synthesized by conventional red-ox polymerization technique. Using this separation medium, capillary electrophoresis of BigDye DNA sequencing standard was performed. A single base resolution of .about.725 bases was achieved in .about.60 minute in a non-covalently coated capillary of 50 .mu.m i.d., 40 cm effective length, and a filed of 160 V/cm at 40.degree. C. The resolution achieved with this formulation to separate DNA under identical conditions is much superior (725 bases vs. 625 bases) and faster (60 min. vs. 75 min.) to the commercially available PAAm, such as supplied by Amersham. The formulation method employed here to synthesize PAAm is straight-forward, simple and does not require cumbersome methods such as emulsion polymerizaiton in order to achieve very high molecular weights. Also, the formulation here does not require separation of PAAm from the reaction mixture prior to reconstituting the polymer to a final concentration. Furthermore, the formulation here is prepared from a single average mol. wt. PAAm as opposed to the mixture of two different average mo. wt. PAAm previously required to achieve high resolution.

  1. Characterization of Traveling Wave Ion Mobility Separations in Structures for Lossless Ion Manipulations

    SciTech Connect (OSTI)

    Hamid, Ahmed M.; Ibrahim, Yehia M.; Garimella, Venkata BS; Webb, Ian K.; Deng, Liulin; Chen, Tsung-Chi; Anderson, Gordon A.; Prost, Spencer A.; Norheim, Randolph V.; Tolmachev, Aleksey V.; Smith, Richard D.

    2015-10-28

    We report on the development and characterization of a new traveling wave-based Structure for Lossless Ion Manipulations (TW-SLIM) for ion mobility separations (IMS). The TW-SLIM module uses a parallel array of RF electrodes for ion confinement, spaced by an array of short DC electrodes to which a TW can be applied to drive ion motion. The ability of the TW-SLIM for efficient ion confinement, lossless ion transport, and ion mobility separations at different RF and TW parameters is reported. The TW-SLIM module is shown to allow transfers a wide mass range of ions (200-2500 Da) utilizing a confining RF waveform (1 MHz and 300 Vp-p), and low TW amplitudes (<20 V). Also, the short module achieved an ion mobility peak capacity of 24 and a peak generation rate of 1014 s-1 for TW speeds of <210 m/s. TW-SLIM performance was characterized over a wide range of RF and DC voltage parameters, and demonstrated robust performance. The combined attributes of flexible design and low voltage requirements for traveling wave IMS, provide a basis for SLIM devices incorporating extended series of ion manipulations.

  2. A3-4 Table A3-1. Classification

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    A3-4 Table A3-1. Classification of soils in Set-Aside Areas. SOIL SOIL TOTAL TOTAL SYMBOL ACRES HECTARES 0/0 AeB 13.21 5.35 0.09% AnB 244.91 99.11 1.75% BaB 952.91 385.64 6.80% BaC 405.76 164.21 2.90% Ch 73.95 29.93 0.53% Da 410.76 166.24 2.93% DoA 73.95 29.93 0.53% DoB 177.04 71.65 1.26% EnA 146.32 59.22 1.04% Fa 1.634.61 661.53 11.67% FuA 44.33 17.94 0.32% FuB 378.95 153.36 2.71% FuC 30.86 12.49 0.22% HoA 78.37 31.71 0.56% LaB 236.53 95.73 1.69% LaC 37.28 15.09 0.27% LuA 37.28 15.09 0.27% LuB

  3. A=12C (68AJ02)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    68AJ02) (See Energy Level Diagrams for 12C) GENERAL: See also Table 12.7 [Table of Energy Levels] (in PDF or PS). Shell model: (KU56, PE56A, KU57A, ME60D, TA60L, WE60B, BA61N, TR61, NA63A, VI63A, AM64, CL64, GI64C, GI64D, NE64C, BA65E, CO65I, FA65C, NE65, GI66A, HA66F, VA66A, YO66A, CO67M, EV67A, KU67B, HI68A). Collective model: (BA59F, BR59M, CL61D, CL62G, GO62I, WA62, GO63G, BR64Z, VO64C, ST65C, UB65, UB65A, VO65A, BO66H, DA66G, DR66B, KR66A, BA67D, BO67D, BO67J, BR67, KR67, LA67F, LA67M,

  4. Determining NO{sub x} emissions from fossil fuel-fired sources

    SciTech Connect (OSTI)

    McNeel, A.

    1996-11-01

    To determine nitrogen oxides (NO{sub x}) emissions, the concentration of NO{sub x} within the stack gas must be determined. USEPA Reference Methods 7, 7A, 7C, 7D and/or 7E are the procedures to be used for NO{sub x} measurement as referenced in 40 CFR 60 subparts D, Da, Db and Dc - {open_quotes}Standards of performance for fossil fuel-fired steam generators...{open_quotes}. Depending upon the reason for determining NO{sub x} emissions, information in addition to NO{sub x} concentrations may be needed. Generally, USEPA Reference Methods 1 - 4 will be used to gather the additional data needed to satisfy the specific need for determining NO{sub x} emissions. The following text outlines the individual NO{sub x} sampling methodology, the relative costs of the reference method (RM) sampling, and the use of the resulting reference method data to calculate emissions in units of applicable standards.

  5. Crystallization and preliminary crystallographic analysis of orotidine 5?-monophosphate decarboxylase from the human malaria parasite Plasmodium falciparum

    SciTech Connect (OSTI)

    Krungkrai, Sudaratana R.; Tokuoka, Keiji; Kusakari, Yukiko; Inoue, Tsuyoshi; Adachi, Hiroaki; Matsumura, Hiroyoshi; Takano, Kazufumi; Murakami, Satoshi; Mori, Yusuke; Kai, Yasushi; Krungkrai, Jerapan; Horii, Toshihiro

    2006-06-01

    Orotidine 5?-monophosphate decarboxylase of human malaria parasite P. falciparum was crystallized by the seeding method in a hanging drop using PEG 3000 as a precipitant. A complete set of diffraction data from a native crystal was collected to 2.7 resolution at 100 K using synchrotron radiation. Orotidine 5?-monophosphate (OMP) decarboxylase (OMPDC; EC 4.1.1.23) catalyzes the final step in the de novo synthesis of uridine 5?-monophosphate (UMP) and defects in the enzyme are lethal in the malaria parasite Plasmodium falciparum. Active recombinant P. falciparum OMPDC (PfOMPDC) was crystallized by the seeding method in a hanging drop using PEG 3000 as a precipitant. A complete set of diffraction data from a native crystal was collected to 2.7 resolution at 100 K using synchrotron radiation at the Swiss Light Source. The crystal exhibits trigonal symmetry (space group R3), with hexagonal unit-cell parameters a = b = 201.81, c = 44.03 . With a dimer in the asymmetric unit, the solvent content is 46% (V{sub M} = 2.3 {sup 3} Da{sup ?1})

  6. Cytometric Catheter for Neurosurgical Applications

    SciTech Connect (OSTI)

    Evans III, Boyd Mccutchen [ORNL; Allison, Stephen W [ORNL; Fillmore, Helen [ORNL; Broaddus, William C [ORNL; Dyer, Rachel L [ORNL; Gillies, George [ORNL

    2010-01-01

    Implantation of neural progenitor cells into the central nervous system has attracted strong interest for treatment of a variety of pathologies. For example, the replacement of dopamine-producing (DA) neural cells in the brain appears promising for the treatment of patients affected by Parkinson's disease. Previous studies of cell-replacement strategies have shown that less than 90% of implanted cells survive longer than 24 - 48 hours following the implantation procedure. However, it is unknown if these cells were viable upon delivery, or if they were affected by other factors such as brain pathology or an immune response. An instrumented cell-delivery catheter has been developed to assist in answering these questions by facilitating quantification and monitoring of the viability of the cells delivered. The catheter uses a fiber optic probe to perform flourescence-based cytometric measurments on cells exiting the port at the catheter tip. The current implementation of this design is on a 3.2 mm diameter catheter with 245 micrometer diameter optical fibers. Results of fluorescence testing data are presented and show that the device can characterize the quantity of cell densities ranging from 60,000 cells/ml to 600,000 cells/ml with a coefficient of determination of 0.93.

  7. Genetic maps of polymorphic DNA loci on rat chromosome 1

    SciTech Connect (OSTI)

    Ding, Yan-Ping; Remmers, E.F.; Longman, R.E.

    1996-09-01

    Genetic linkage maps of loci defined by polymorphic DNA markers on rat chromosome 1 were constructed by genotyping F2 progeny of F344/N x LEW/N, BN/SsN x LEW/N, and DA/Bkl x F344/Hsd inbred rat strains. In total, 43 markers were mapped, of which 3 were restriction fragment length polymorphisms and the others were simple sequence length polymorphisms. Nineteen of these markers were associated with genes. Six markers for five genes, {gamma}-aminobutyric acid receptor {beta}3 (Gabrb3), syntaxin 2 (Stx2), adrenergic receptor {beta}3 (Gabrb3), syntaxin 2 (Stx2), adrenergic receptor {beta}1 (Adrb1), carcinoembryonic antigen gene family member 1 (Cgm1), and lipogenic protein S14 (Lpgp), and 20 anonymous loci were not previously reported. Thirteen gene loci (Myl2, Aldoa, Tnt, Igf2, Prkcg, Cgm4, Calm3, Cgm3, Psbp1, Sa, Hbb, Ins1, and Tcp1) were previously mapped. Comparative mapping analysis indicated that the large portion of rat chromosome 1 is homologous to mouse chromosome 7, although the homologous to mouse chromosome 7, although the homologs of two rat genes are located on mouse chromosomes 17 and 19. Homologs of the rat chromosome 1 genes that we mapped are located on human chromosomes 6, 10, 11, 12, 15, 16, and 19. 38 refs., 1 fig., 3 tabs.

  8. EsxB, a secreted protein from Bacillus anthracis forms two distinct helical bundles

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Fan, Yao; Tan, Kemin; Chhor, Gekleng; Butler, Emily K.; Jedrzejczak, Robert P.; Missiakas, Dominique; Joachimiak, Andrzej

    2015-07-03

    The EsxB protein from Bacillus anthracis belongs to the WXG100 family, a group of proteins secreted by a specialized secretion system. We have determined the crystal structures of recombinant EsxB and discovered that the small protein (~10 kDa), comprised of a helix-loop-helix (HLH) hairpin, is capable of associating into two different helical bundles. The two basic quaternary assemblies of EsxB are an antiparallel (AP) dimer and a rarely observed bisecting U (BU) dimer. This structural duality of EsxB is believed to originate from the heptad repeat sequence diversity of the first helix of its HLH hairpin, which allows for twomore » alternative helix packing. The flexibility of EsxB and the ability to form alternative helical bundles underscore the possibility that this protein can serve as an adaptor in secretion and can form hetero-oligomeric helix bundle(s) with other secreted members of the WXG100 family, such as EsxW. The highly conserved WXG motif is located within the loop of the HLH hairpin and is mostly buried within the helix bundle suggesting that its role is mainly structural. The exact functions of the motif, including a proposed role as a secretion signal, remain unknown.« less

  9. A multi-channel gel electrophoresis and continuous fraction collection apparatus for high throughput protein separation and characterization

    SciTech Connect (OSTI)

    Choi, Megan; Nordmeyer, Robert A.; Cornell, Earl; Dong, Ming; Biggin, Mark D.; Jin, Jian

    2009-10-02

    To facilitate a direct interface between protein separation by PAGE and protein identification by mass spectrometry, we developed a multichannel system that continuously collects fractions as protein bands migrate off the bottom of gel electrophoresis columns. The device was constructed using several short linear gel columns, each of a different percent acrylamide, to achieve a separation power similar to that of a long gradient gel. A Counter Free-Flow elution technique then allows continuous and simultaneous fraction collection from multiple channels at low cost. We demonstrate that rapid, high-resolution separation of a complex protein mixture can be achieved on this system using SDS-PAGE. In a 2.5 h electrophoresis run, for example, each sample was separated and eluted into 48-96 fractions over a mass range of 10-150 kDa; sample recovery rates were 50percent or higher; each channel was loaded with up to 0.3 mg of protein in 0.4 mL; and a purified band was eluted in two to three fractions (200 L/fraction). Similar results were obtained when running native gel electrophoresis, but protein aggregation limited the loading capacity to about 50 g per channel and reduced resolution.

  10. Detection of three nonsense mutations and one missense mutation in the interleukin-2 receptor [gamma] chain gene in SCIDX1 that differently affect the mRNA processing

    SciTech Connect (OSTI)

    Markiewicz, S.; Fischer, A.; Saint Basile, G. de ); Subtil, A.; Dautry-Varsat, A. )

    1994-05-01

    The interleukin-2 receptor [gamma] (IL-2R[gamma]) chain gene encodes a 64-kDa protein that not only composes the high-affinity form of the IL-2 binding receptor in association with the 2R [alpha] and [beta] chains, but also participates in at least the IL-4 and IL-7 receptor complexes. Mutations in this gene have recently been shown to cause X-linked severe combined immunodeficiency (SCIDX1). This disease of the immune system results from an early block of T lymphocyte and natural killer (NK) cell differentiation, which leads to a severe cellular and humoral immune defect that is lethal unless treated by bone marrow transplantation. Analysis of the IL-2R[gamma] gene in SCIDX1 patients has revealed the presence of heterogeneous mutations principally located in the extracellular domain of the molecule. We report here three intraexonic mutations and one deletion in the IL-2R[gamma] gene in four SCIDX1 patients. These mutations appear to differentially affect RNA processing, either by decreasing IL-2R[gamma] mRNA level or by the skipping of a constitutive exon. 16 refs., 1 fig.

  11. Crystallization and preliminary X-ray crystallographic studies of fatty acid-CoA racemase from Mycobacterium tuberculosis H37Rv

    SciTech Connect (OSTI)

    Rhee, Kyung-Hee; Lee, Ki Seog; Priyadarshi, Amit; Kim, Eunice Eunkyung; Hwang, Kwang Yeon

    2005-11-01

    Fatty acid-CoA racemase from M. tuberculosis H37Rv has been overexpressed, purified and crystallized. Diffraction data have been collected to beyond 2.7 resolution using a synchrotron-radiation source. Fatty acid-CoA racemase plays an important role in the ?-oxidation of branched-chain fatty acids and fatty-acid derivatives as it catalyzes the conversion of several (2R)-branched-chain fatty acid-CoAs to their (2S)-stereoisomers. Fatty acid-CoA racemase from Mycobacterium tuberculosis H37Rv has been purified to homogeneity and crystallized by the hanging-drop vapour-diffusion method with polyethylene glycol 4000 as precipitant. The crystals belong to the trigonal space group P3{sub 1} or P3{sub 2}, with unit-cell parameters a = b = 109.56, c = 147.97 . The asymmetric unit contains six monomers, corresponding to a V{sub M} value of 2.15 {sup 3} Da{sup ?1}. A complete native data set has been collected at 2.7 resolution using a synchrotron-radiation source.

  12. Spherically symmetric solutions, Newton's Law, and the infrared limit {lambda}{yields}1 in covariant Horava-Lifshitz gravity

    SciTech Connect (OSTI)

    Alexandre, Jean; Pasipoularides, Pavlos

    2011-10-15

    In this note we examine whether spherically symmetric solutions in covariant Horava-Lifshitz gravity can reproduce Newton's Law in the IR limit {lambda}{yields}1. We adopt the position that the auxiliary field A is independent of the space-time metric [J. Alexandre and P. Pasipoularides, Phys. Rev. D 83, 084030 (2011).][J. Greenwald, V. H. Satheeshkumar, and A. Wang, J. Cosmol. Astropart. Phys. 12 (2010) 007.], and we assume, as in [A. M. da Silva, Classical Quantum Gravity 28, 055011 (2011).], that {lambda} is a running coupling constant. We show that under these assumptions, spherically symmetric solutions fail to restore the standard Newtonian physics in the IR limit {lambda}{yields}1, unless {lambda} does not run, and has the fixed value {lambda}=1. Finally, we comment on the Horava and Melby-Thompson approach [P. Horava and C. M. Melby-Thompson, Phys. Rev. D 82, 064027 (2010).] in which A is assumed as a part of the space-time metric in the IR.

  13. Crystal Structure of the Monomeric Porin OmpG

    SciTech Connect (OSTI)

    Subbarao,G.; van den Berg, B.

    2006-01-01

    The outer membrane (OM) of Gram-negative bacteria contains a large number of channel proteins that mediate the uptake of ions and nutrients necessary for growth and functioning of the cell. An important group of OM channel proteins are the porins, which mediate the non-specific, diffusion-based passage of small (<600 Da) polar molecules. All porins of Gram-negative bacteria that have been crystallized to date form stable trimers, with each monomer composed of a 16-stranded {beta}-barrel with a relatively narrow central pore. In contrast, the OmpG porin is unique, as it appears to function as a monomer. We have determined the X-ray crystal structure of OmpG from Escherichia coli to a resolution of 2.3 Angstroms. The structure shows a 14-stranded {beta}{beta}-barrel with a relatively simple architecture. Due to the absence of loops that fold back into the channel, OmpG has a large ({approx}13 Angstroms) central pore that is considerably wider than those of other E. coli porins, and very similar in size to that of the toxin a-hemolysin. The architecture of the channel, together with previous biochemical and other data, suggests that OmpG may form a non-specific channel for the transport of larger oligosaccharides. The structure of OmpG provides the starting point for engineering studies aiming to generate selective channels and for the development of biosensors.

  14. Unified Einstein-Virasoro Master Equation in the General Non-Linear Sigma Model

    SciTech Connect (OSTI)

    Boer, J. de; Halpern, M.B.

    1996-06-05

    The Virasoro master equation (VME) describes the general affine-Virasoro construction $T=L^abJ_aJ_b+iD^a \\dif J_a$ in the operator algebra of the WZW model, where $L^ab$ is the inverse inertia tensor and $D^a $ is the improvement vector. In this paper, we generalize this construction to find the general (one-loop) Virasoro construction in the operator algebra of the general non-linear sigma model. The result is a unified Einstein-Virasoro master equation which couples the spacetime spin-two field $L^ab$ to the background fields of the sigma model. For a particular solution $L_G^ab$, the unified system reduces to the canonical stress tensors and conventional Einstein equations of the sigma model, and the system reduces to the general affine-Virasoro construction and the VME when the sigma model is taken to be the WZW action. More generally, the unified system describes a space of conformal field theories which is presumably much larger than the sum of the general affine-Virasoro construction and the sigma model with its canonical stress tensors. We also discuss a number of algebraic and geometrical properties of the system, including its relation to an unsolved problem in the theory of $G$-structures on manifolds with torsion.

  15. Army energy plan

    SciTech Connect (OSTI)

    Not Available

    1980-08-08

    The Army Energy Plan describes the current and projected (to the year 2000) energy situation in which the Department of the Army (DA) must operate and summarizes those actions and programs which have been developed and/or are needed to cope with those conditions. It addresses the Army goals, objectives, policies, and programs for all Army activities. World industrial growth during the past century has been characterized and hastened by the widespread availability of inexpensive energy, primarily petroleum. The Arab oil embargo of 1973 served to drive home a number of points, key among them being that the world's principal oil consumers are not the major oil producers. The Middle East and Africa have an estimated 67 percent of the petroleum reserves while Western Europe and the Western Hemisphere have only 16 percent. By most estimates, these reserves are expected to be exhausted within the next 70 years. In the year following the 1973 oil embargo, prices for petroleum rose threefold, signaling the end of the cheap oil. In the decade of the seventies, the price of imported crude oil has risen from $1.80 per barrel to an exorbitant $30.00 per barrel. The combined threats of exhaustion and high cost mandate the use of alternate sources of energy. The distribution of alternate sources of recoverable oil, such as tar sands and oil shale, favor the Western Hemisphere, but economical recovery techniques have not been developed.

  16. Structural and functional characterization of the enantiomers of the antischistosomal drug oxamniquine

    SciTech Connect (OSTI)

    Taylor, Alexander B.; Pica-Mattoccia, Livia; Polcaro, Chiara M.; Donati, Enrica; Cao, Xiaohang; Basso, Annalisa; Guidi, Alessandra; Rugel, Anastasia R.; Holloway, Stephen P.; Anderson, Timothy J.C.; Hart, P. John; Cioli, Donato; LoVerde, Philip T.

    2015-10-20

    For over two decades, a racemic mixture of oxamniquine (OXA) was administered to patients infected by Schistosoma mansoni, but whether one or both enantiomers exert antischistosomal activity was unknown. Recently, a ~30 kDa S. mansoni sulfotransferase (SmSULT) was identified as the target of OXA action. Here, we separate the OXA enantiomers using chromatographic methods and assign their optical activities as dextrorotary [(+)-OXA] or levorotary [(-)-OXA]. Crystal structures of the parasite enzyme in complex with optically pure (+)-OXA and (-)-OXA) reveal their absolute configurations as S- and R-, respectively. When tested in vitro, S-OXA demonstrated the bulk of schistosomicidal activity, while R-OXA had antischistosomal effects when present at relatively high concentrations. Crystal structures R-OXA•SmSULT and S-OXA•SmSULT complexes reveal similarities in the modes of OXA binding, but only the S-OXA enantiomer is observed in the structure of the enzyme exposed to racemic OXA. Together the data suggest the higher schistosomicidal activity of S-OXA is correlated with its ability to outcompete R-OXA binding the sulfotransferase active site. In conclusion, these findings have important implications for the design, syntheses, and dosing of new OXA-based antischistosomal compounds.

  17. Purification and crystallization of a trimodular complex comprising the type II cohesin–dockerin interaction from the cellulosome of Clostridium thermocellum

    SciTech Connect (OSTI)

    Adams, Jarrett J.; Pal, Gour; Yam, Katherine; Spencer, Holly L.; Jia, Zongchao; Smith, Steven P.

    2005-01-01

    A trimodular complex comprising the type II cohesin–dockerin interaction from the cellulosome of C. thermocellum has been purified and crystallized by the hanging-drop vapour-diffusion method. A native crystal and a selenomethionine derivative have been analyzed using X-ray diffraction. The high-affinity calcium-mediated type II cohesin–dockerin interaction is responsible for the attachment of the multi-enzyme cellulose-degrading complex, termed the cellulosome, to the cell surface of the thermophilic anaerobe Clostridium thermocellum. A trimodular 40 kDa complex comprising the SdbA type II cohesin and the the CipA type II dockerin–X module modular pair from the cellulosome of C. thermocellum has been crystallized. The crystals belong to space group P2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 45.21, b = 52.34, c = 154.69 Å. The asymmetric unit contains one molecule of the protein complex and native and selenomethionine-derivative crystals diffracted to 2.1 and 2.0 Å, respectively.

  18. Advanced Recombinant Manganese Peroxidase for Biosynthesis of Lignin Bioproducts, Phase I Final Report, STTR Grant #: DE-SC0007503.

    SciTech Connect (OSTI)

    Beatty, Christopher; Kitner, Joshua; Lajoie, Curtis; McClain, Sean; Potochnik, Steve

    2012-12-13

    The core purpose of this Phase I STTR was to evaluate the feasibility of a new method of producing a recombinant version of manganese peroxidase (MnP) enzyme. MnP is a potentially valuable enzyme for producing high value lignin products and also for industrial de-coloring operations such as biobleaching of pulp and color removal from textile dye effluents. This lignin-modifying enzyme is produced in small amounts by the native host, a white rot fungus. Previous work by Oregon State University developed a secreted recombinant version of the enzyme in the yeast Pichia pastoris. Unfortunately, the expression is barely moderate and the enzyme is heavily glycosylated, which inhibits purification. In this work, the gene for the enzyme is given a tag which targets production of the enzyme to the peroxisome. This is a promising approach since this location is also where heme and hydrogen peroxide are sequestered, which are both necessary cofactors for MnP. More than ten recombinant strains were constructed, verified, and expressed in the Pichia system. Constitutive (GAP) and methanol-induced promoters (AOX) were tried for peroxisomal targeted, cytosolic, and secreted versions of MnP. Only the secreted strains showed activity. The amount of expression was not significantly changed. The degree of glycosylation was lessened using the AOX (methanol) promotoer, but the resulting enzyme was still not able to be purified using immobilized metal affinity chromatography. Additional work beyond the scope of the defined Phase I project was undertaken to construct, verify, and express Pichia strains that mutated the MnP glycosylation sites to inhibit this process. These strains did not show significant activity. The cause is not known, but it is possible that these sites are important to the structure of the enzyme. Also beyond the scope proposed for our Phase I STTR, the team collaborated with AbSci, a startup with a new E. coli based expression system focused on the production of antibodies and enzymes containing disulfide bonds and requiring folding/post-translational modification. With only limited time remaining in the Phase I schedule, a single construct was made to produce MnP with this system. The enzyme was produced in the soluble fraction of the cell lysate, but no activity was measured. MnP from the existing recombinant source was used to act on lignin. The lignin was from a Kraft process and had a molecular weight of about 10,000 Da. Using 1000 Da dialysis membranes and UV-visible spectroscopy, no modification of either lignin was evident in the dialysate or the retentate. Assays using 2,6 dimethoxy phenol (DMP) as a substrate showed consistent activity throughout the project. In summary, these results fell far short of our expectations. A Phase II proposal was not submitted. Possible reasons for the failure of peroxisomal targeting include destruction by native hydrogen peroxide, native proteases, or unforeseen causes. The AbSci system was only lighted tested and further work may yield a strain with active enzyme. The lack of evidence for lignin modification may be due to the techniques employed. NMR or GC-MS studies may reveal evidence of modification.

  19. Crystal structure of riboflavin synthase

    SciTech Connect (OSTI)

    Liao, D.-I.; Wawrzak, Z.; Calabrese, J.C.; Viitanen, P.V.; Jordan, D.B.

    2010-03-05

    Riboflavin synthase catalyzes the dismutation of two molecules of 6,7-dimethyl-8-(1'-D-ribityl)-lumazine to yield riboflavin and 4-ribitylamino-5-amino-2,6-dihydroxypyrimidine. The homotrimer of 23 kDa subunits has no cofactor requirements for catalysis. The enzyme is nonexistent in humans and is an attractive target for antimicrobial agents of organisms whose pathogenicity depends on their ability to biosynthesize riboflavin. The first three-dimensional structure of the enzyme was determined at 2.0 {angstrom} resolution using the multiwavelength anomalous diffraction (MAD) method on the Escherichia coli protein containing selenomethionine residues. The homotrimer consists of an asymmetric assembly of monomers, each of which comprises two similar {beta} barrels and a C-terminal {alpha} helix. The similar {beta} barrels within the monomer confirm a prediction of pseudo two-fold symmetry that is inferred from the sequence similarity between the two halves of the protein. The {beta} barrels closely resemble folds found in phthalate dioxygenase reductase and other flavoproteins. The three active sites of the trimer are proposed to lie between pairs of monomers in which residues conserved among species reside, including two Asp-His-Ser triads and dyads of Cys-Ser and His-Thr. The proposed active sites are located where FMN (an analog of riboflavin) is modeled from an overlay of the {beta} barrels of phthalate dioxygenase reductase and riboflavin synthase. In the trimer, one active site is formed, and the other two active sites are wide open and exposed to solvent. The nature of the trimer configuration suggests that only one active site can be formed and be catalytically competent at a time.

  20. Nucleon-gold collisions at 200A GeV using tagged d + Au interactions in the PHOBOS detector

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Back, B. B.; Nouicer, R.; Baker, M. D.; Ballintijn, M.; Barton, D. S.; Becker, B.; Betts, R. R.; Bickley, A. A; Stienberg, P.; Ioradnova, A.; et al

    2015-09-23

    Forward calorimetry in the PHOBOS detector has been used to study charged hadron production in d+Au, p+Au, and n+Au collisions at √sNN =200GeV. The forward proton calorimeter detectors are described and a procedure for determining collision centrality with these detectors is detailed. The deposition of energy by deuteron spectator nucleons in the forward calorimeters is used to identify p+Au and n+Au collisions in the data. A weighted combination of the yield of p+Au and n+Au is constructed to build a reference for Au+Au collisions that better matches the isospin composition of the gold nucleus. The pT and centrality dependence ofmore » the yield of this improved reference system is found to match that of d+Au. The shape of the charged-particle transverse momentum distribution is observed to extrapolate smoothly from p+p¯ to central d+Au as a function of the charged-particle pseudorapidity density. The asymmetry of positively and negatively charged hadron production in p+Au is compared to that of n+Au. No significant asymmetry is observed at midrapidity. In conclusion, these studies augment recent results from experiments at the CERN Large Hadron Collider and BNL Relativistic Heavy Ion Collider facilities to give a more complete description of particle production in p+A and d+A collisions, essential for the understanding the medium produced in high-energy nucleus-nucleus collisions.« less

  1. Cloning and characterization of GDP-perosamine synthetase (Per) from Escherichia coli O157:H7 and synthesis of GDP-perosamine in vitro

    SciTech Connect (OSTI)

    Zhao Guohui; Liu Jun; Liu Xiang; Chen Min; Zhang Houcheng Wang, Peng George

    2007-11-23

    GDP-perosamine synthetase (Per, E.C. not yet classified) is important to the synthesis of Escherichia coli O157:H7 O-antigen. The mutant in per gene can disrupt the synthesis of O157 O-antigen. In this study, GDP-perosamine synthetase was cloned from E. coli O157:H7 and over-expressed in E. coli BL21 (DE3). The recombinant His-tagged Per fusion protein was a decamer with molecular weight of 431 kDa. The optimal pH value of this recombinant protein was 7.5. The divalent ions had no significant effect on Per-catalyzed reaction. The K{sub m} and K{sub cat}/K{sub m} for GDP-4-keto-6-deoxy-D-mannose were 0.09 mM and 2.1 x 10{sup 5} M{sup -1} S{sup -1}, and those for L-glutamate were 2 mM and 0.52 x 10{sup 5} M{sup -1}S{sup -1}, respectively. Per was used to synthesize GDP-perosamine from GDP-mannose together with recombinant GDP-mannose dehydratase (GMD, E.C. 4.2.1.47). The purified GDP-perosamine was identified by MS and NMR. In summary, this work provided a feasible approach for the synthesis of GDP-perosamine which can lead to the study of LPS biosynthesis of pathogenic E. coli O157:H7.

  2. Impaired methylation as a novel mechanism for proteasome suppression in liver cells

    SciTech Connect (OSTI)

    Osna, Natalia A.; White, Ronda L.; Donohue, Terrence M.; Department of Internal Medicine, University of Nebraska Medical Center, Omaha, NE 68105 ; Beard, Michael R.; Tuma, Dean J.; Kharbanda, Kusum K.; Department of Internal Medicine, University of Nebraska Medical Center, Omaha, NE 68105

    2010-01-08

    The proteasome is a multi-catalytic protein degradation enzyme that is regulated by ethanol-induced oxidative stress; such suppression is attributed to CYP2E1-generated metabolites. However, under certain conditions, it appears that in addition to oxidative stress, other mechanisms are also involved in proteasome regulation. This study investigated whether impaired protein methylation that occurs during exposure of liver cells to ethanol, may contribute to suppression of proteasome activity. We measured the chymotrypsin-like proteasome activity in Huh7CYP cells, hepatocytes, liver cytosols and nuclear extracts or purified 20S proteasome under conditions that maintain or prevent protein methylation. Reduction of proteasome activity of hepatoma cell and hepatocytes by ethanol or tubercidin was prevented by simultaneous treatment with S-adenosylmethionine (SAM). Moreover, the tubercidin-induced decline in proteasome activity occurred in both nuclear and cytosolic fractions. In vitro exposure of cell cytosolic fractions or highly purified 20S proteasome to low SAM:S-adenosylhomocysteine (SAH) ratios in the buffer also suppressed proteasome function, indicating that one or more methyltransferase(s) may be associated with proteasomal subunits. Immunoblotting a purified 20S rabbit red cell proteasome preparation using methyl lysine-specific antibodies revealed a 25 kDa proteasome subunit that showed positive reactivity with anti-methyl lysine. This reactivity was modified when 20S proteasome was exposed to differential SAM:SAH ratios. We conclude that impaired methylation of proteasome subunits suppressed proteasome activity in liver cells indicating an additional, yet novel mechanism of proteasome activity regulation by ethanol.

  3. Nuclear localization of DMP1 proteins suggests a role in intracellular signaling

    SciTech Connect (OSTI)

    Siyam, Arwa; Department of Endodontology, Kornberg School of Dentistry, Temple University, 3223 North Broad Street, Philadelphia, PA 19140-5007 ; Wang, Suzhen; Qin, Chunlin; Mues, Gabriele; Stevens, Roy; D'Souza, Rena N.; Lu, Yongbo

    2012-08-03

    Highlights: Black-Right-Pointing-Pointer Nuclear localization of DMP1 in various cell lines. Black-Right-Pointing-Pointer Non-synchronized cells show either nuclear or cytoplasmic localization of DMP1. Black-Right-Pointing-Pointer Nuclear DMP1 is restricted to the nucleoplasm but absent in the nucleolus. -- Abstract: Dentin matrix protein 1 (DMP1) is highly expressed in odontoblasts and osteoblasts/osteocytes and plays an essential role in tooth and bone mineralization and phosphate homeostasis. It is debatable whether DMP1, in addition to its function in the extracellular matrix, can enter the nucleus and function as a transcription factor. To better understand its function, we examined the nuclear localization of endogenous and exogenous DMP1 in C3H10T1/2 mesenchymal cells, MC3T3-E1 preosteoblast cells and 17IIA11 odontoblast-like cells. RT-PCR analyses showed the expression of endogenous Dmp1 in all three cell lines, while Western-blot analysis detected a major DMP1 protein band corresponding to the 57 kDa C-terminal fragment generated by proteolytic processing of the secreted full-length DMP1. Immunofluorescent staining demonstrated that non-synchronized cells presented two subpopulations with either nuclear or cytoplasmic localization of endogenous DMP1. In addition, cells transfected with a construct expressing HA-tagged full-length DMP1 also showed either nuclear or cytoplasmic localization of the exogenous DMP1 when examined with an antibody against the HA tag. Furthermore, nuclear DMP1 was restricted to the nucleoplasm but was absent in the nucleolus. In conclusion, these findings suggest that, apart from its role as a constituent of dentin and bone matrix, DMP1 might play a regulatory role in the nucleus.

  4. Characterization of MtoD from Sideroxydans lithotrophicus: a cytochrome c electron shuttle used in lithoautotrophic growth

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Beckwith, Christopher R.; Edwards, Marcus J.; Lawes, Matthew; Shi, Liang; Butt, Julea N.; Richardson, David J.; Clarke, Thomas A.

    2015-04-28

    The autotrophic Sideroxydans lithotrophicus ES-1 can grow by coupling the oxidation of ferrous iron to the reduction of oxygen. Soluble ferrous iron is oxidized at the surface of the cell by an MtoAB porin-cytochrome complex that functions as an electron conduit through the outer membrane. Electrons are then transported to the cytoplasmic membrane where they are used to generate proton motive force (PMF) (for ATP synthesis) and NADH for autotrophic processes such as carbon fixation. As part of the mtoAB gene cluster, S. lithotrophicus also contains the gene mtoD that is proposed to encode a cytochrome c protein. We isolatedmore » mtoD from a Shewanella oneidensis expression system where the mtoD gene was expressed on a pBAD plasmid vector. Biochemical, biophysical, and crystallographic characterization of the purified MtoD revealed it as an 11 kDa monomeric protein containing a single heme. Sequence and structural alignment indicated that MtoD belonged to the class-1 cytochrome c family and had a similar fold to ferricytochrome c552 family, however the MtoD heme is bis-histidine coordinated and is substantially more exposed than the hemes of other family members. The reduction potential of the MtoD heme at pH 7 was +155 mV vs. Standard Hydrogen Electrode, which is approximately 100 mV lower than that of mitochondrial cytochrome c. Consideration of the properties of MtoD in the context of the potential respiratory partners identified from the genome suggests that MtoD could associate to multiple electron transfer partners as the primary periplasmic electron shuttle.« less

  5. Wide cool and ultracool companions to nearby stars from Pan-STARRS 1

    SciTech Connect (OSTI)

    Deacon, Niall R.; Liu, Michael C.; Magnier, Eugene A.; Aller, Kimberly M.; Best, William M. J.; Bowler, Brendan P.; Burgett, William S.; Chambers, Kenneth C.; Flewelling, H.; Kaiser, Nick; Kudritzki, Rolf-Peter; Morgan, Jeff S.; Tonry, John L.; Dupuy, Trent; Mann, Andrew W.; Redstone, Joshua A.; Draper, Peter W.; Metcalfe, Nigel; Hodapp, Klaus W.; Price, Paul A.; and others

    2014-09-10

    We present the discovery of 57 wide (>5'') separation, low-mass (stellar and substellar) companions to stars in the solar neighborhood identified from Pan-STARRS 1 (PS1) data and the spectral classification of 31 previously known companions. Our companions represent a selective subsample of promising candidates and span a range in spectral type of K7-L9 with the addition of one DA white dwarf. These were identified primarily from a dedicated common proper motion search around nearby stars, along with a few as serendipitous discoveries from our Pan-STARRS 1 brown dwarf search. Our discoveries include 23 new L dwarf companions and one known L dwarf not previously identified as a companion. The primary stars around which we searched for companions come from a list of bright stars with well-measured parallaxes and large proper motions from the Hipparcos catalog (8583 stars, mostly A-K dwarfs) and fainter stars from other proper motion catalogs (79170 stars, mostly M dwarfs). We examine the likelihood that our companions are chance alignments between unrelated stars and conclude that this is unlikely for the majority of the objects that we have followed-up spectroscopically. We also examine the entire population of ultracool (>M7) dwarf companions and conclude that while some are loosely bound, most are unlikely to be disrupted over the course of ?10 Gyr. Our search increases the number of ultracool M dwarf companions wider than 300 AU by 88% and increases the number of L dwarf companions in the same separation range by 82%. Finally, we resolve our new L dwarf companion to HIP 6407 into a tight (0.''13, 7.4 AU) L1+T3 binary, making the system a hierarchical triple. Our search for these key benchmarks against which brown dwarf and exoplanet atmosphere models are tested has yielded the largest number of discoveries to date.

  6. CALIBRATION OF THE MIXING-LENGTH THEORY FOR CONVECTIVE WHITE DWARF ENVELOPES

    SciTech Connect (OSTI)

    Tremblay, P.-E.; Ludwig, H.-G.; Freytag, B.; Fontaine, G.; Brassard, P.; Steffen, M.

    2015-02-01

    A calibration of the mixing-length parameter in the local mixing-length theory (MLT) is presented for the lower part of the convection zone in pure-hydrogen-atmosphere white dwarfs. The parameterization is performed from a comparison of three-dimensional (3D) CO5BOLD simulations with a grid of one-dimensional (1D) envelopes with a varying mixing-length parameter. In many instances, the 3D simulations are restricted to the upper part of the convection zone. The hydrodynamical calculations suggest, in those cases, that the entropy of the upflows does not change significantly from the bottom of the convection zone to regions immediately below the photosphere. We rely on this asymptotic entropy value, characteristic of the deep and adiabatically stratified layers, to calibrate 1D envelopes. The calibration encompasses the convective hydrogen-line (DA) white dwarfs in the effective temperature range 6000 ≤ T {sub eff} (K) ≤15, 000 and the surface gravity range 7.0 ≤ log g ≤ 9.0. It is established that the local MLT is unable to reproduce simultaneously the thermodynamical, flux, and dynamical properties of the 3D simulations. We therefore propose three different parameterizations for these quantities. The resulting calibration can be applied to structure and envelope calculations, in particular for pulsation, chemical diffusion, and convective mixing studies. On the other hand, convection has no effect on the white dwarf cooling rates until there is a convective coupling with the degenerate core below T {sub eff} ∼ 5000 K. In this regime, the 1D structures are insensitive to the MLT parameterization and converge to the mean 3D results, hence they remain fully appropriate for age determinations.

  7. The TRIPOD e-learning Platform for the Training of Earthquake Safety Assessment

    SciTech Connect (OSTI)

    Coppari, S.; Di Pasquale, G.; Goretti, A.; Papa, F.; Papa, S.; Paoli, G.; Pizza, A. G.; Severino, M.

    2008-07-08

    The paper summarizes the results of the in progress EU Project titled TRIPOD (Training Civil Engineers on Post-Earthquake Safety Assessment of Damaged Buildings), funded under the Leonardo Da Vinci program. The main theme of the project is the development of a methodology and a learning platform for the training of technicians involved in post-earthquake building safety inspections. In the event of a catastrophic earthquake, emergency building inspections constitute a major undertaking with severe social impact. Given the inevitable chaotic conditions and the urgent need of a great number of specialized individuals to carry out inspections, past experience indicates that inspection teams are often formed in an adhoc manner, under stressful conditions, at a varying levels of technical expertise and experience, sometime impairing the reliability and consistency of the inspection results. Furthermore each Country has its own building damage and safety assessment methodology, developed according to its experience, laws, building technology and seismicity. This holds also for the partners participating to the project (Greece, Italy, Turkey, Cyprus), that all come from seismically sensitive Mediterranean countries. The project aims at alleviating the above shortcomings by designing and developing a training methodology and e-platform, forming a complete training program targeted at inspection engineers, specialized personnel and civil protection agencies. The e-learning platform will provide flexible and friendly authoring mechanisms, self-teaching and assessment capabilities, course and trainee management, etc. Courses will be also made available as stand-alone multimedia applications on CD and in the form of a complete pocket handbook. Moreover the project will offer the possibility of upgrading different experiences and practices: a first step towards the harmonization of methodologies and tools of different Countries sharing similar problems. Finally, through wide dissemination activities, the final aim of the project is to ensure the deployment and the integration into existing earthquake mitigation policies and vocational training schemes.

  8. Nucleon-gold collisions at 200A GeV using tagged d + Au interactions in the PHOBOS detector

    SciTech Connect (OSTI)

    Back, B. B.; Nouicer, R.; Baker, M. D.; Ballintijn, M.; Barton, D. S.; Becker, B.; Betts, R. R.; Bickley, A. A; Stienberg, P.; Ioradnova, A.; Pak, R.; Sukhanov, A.

    2015-09-23

    Forward calorimetry in the PHOBOS detector has been used to study charged hadron production in d+Au, p+Au, and n+Au collisions at √sNN =200GeV. The forward proton calorimeter detectors are described and a procedure for determining collision centrality with these detectors is detailed. The deposition of energy by deuteron spectator nucleons in the forward calorimeters is used to identify p+Au and n+Au collisions in the data. A weighted combination of the yield of p+Au and n+Au is constructed to build a reference for Au+Au collisions that better matches the isospin composition of the gold nucleus. The pT and centrality dependence of the yield of this improved reference system is found to match that of d+Au. The shape of the charged-particle transverse momentum distribution is observed to extrapolate smoothly from p+p¯ to central d+Au as a function of the charged-particle pseudorapidity density. The asymmetry of positively and negatively charged hadron production in p+Au is compared to that of n+Au. No significant asymmetry is observed at midrapidity. In conclusion, these studies augment recent results from experiments at the CERN Large Hadron Collider and BNL Relativistic Heavy Ion Collider facilities to give a more complete description of particle production in p+A and d+A collisions, essential for the understanding the medium produced in high-energy nucleus-nucleus collisions.

  9. Characterization of MtoD from Sideroxydans lithotrophicus: a cytochrome c electron shuttle used in lithoautotrophic growth

    SciTech Connect (OSTI)

    Beckwith, Christopher R.; Edwards, Marcus J.; Lawes, Matthew; Shi, Liang; Butt, Julea N.; Richardson, David J.; Clarke, Thomas A.

    2015-04-28

    The autotrophic Sideroxydans lithotrophicus ES-1 can grow by coupling the oxidation of ferrous iron to the reduction of oxygen. Soluble ferrous iron is oxidized at the surface of the cell by an MtoAB porin-cytochrome complex that functions as an electron conduit through the outer membrane. Electrons are then transported to the cytoplasmic membrane where they are used to generate proton motive force (PMF) (for ATP synthesis) and NADH for autotrophic processes such as carbon fixation. As part of the mtoAB gene cluster, S. lithotrophicus also contains the gene mtoD that is proposed to encode a cytochrome c protein. We isolated mtoD from a Shewanella oneidensis expression system where the mtoD gene was expressed on a pBAD plasmid vector. Biochemical, biophysical, and crystallographic characterization of the purified MtoD revealed it as an 11 kDa monomeric protein containing a single heme. Sequence and structural alignment indicated that MtoD belonged to the class-1 cytochrome c family and had a similar fold to ferricytochrome c552 family, however the MtoD heme is bis-histidine coordinated and is substantially more exposed than the hemes of other family members. The reduction potential of the MtoD heme at pH 7 was +155 mV vs. Standard Hydrogen Electrode, which is approximately 100 mV lower than that of mitochondrial cytochrome c. Consideration of the properties of MtoD in the context of the potential respiratory partners identified from the genome suggests that MtoD could associate to multiple electron transfer partners as the primary periplasmic electron shuttle.

  10. Effect of intranasal manganese administration on neurotransmission and spatial learning in rats

    SciTech Connect (OSTI)

    Blecharz-Klin, Kamilla; Piechal, Agnieszka; Joniec-Maciejak, Ilona; Pyrzanowska, Justyna; Widy-Tyszkiewicz, Ewa

    2012-11-15

    The effect of intranasal manganese chloride (MnCl{sub 2}4H{sub 2}O) exposure on spatial learning, memory and motor activity was estimated in Morris water maze task in adult rats. Three-month-old male Wistar rats received for 2 weeks MnCl{sub 2}4H{sub 2}O at two doses the following: 0.2 mg/kg b.w. (Mn0.2) or 0.8 mg/kg b.w. (Mn0.8) per day. Control (Con) and manganese-exposed groups were observed for behavioral performance and learning in water maze. ANOVA for repeated measurements did not show any significant differences in acquisition in the water maze between the groups. However, the results of the probe trial on day 5, exhibited spatial memory deficits following manganese treatment. After completion of the behavioral experiment, the regional brain concentrations of neurotransmitters and their metabolites were determined via HPLC in selected brain regions, i.e. prefrontal cortex, hippocampus and striatum. ANOVA demonstrated significant differences in the content of monoamines and metabolites between the treatment groups compared to the controls. Negative correlations between platform crossings on the previous platform position in Southeast (SE) quadrant during the probe trial and neurotransmitter turnover suggest that impairment of spatial memory and cognitive performance after manganese (Mn) treatment is associated with modulation of the serotonergic, noradrenergic and dopaminergic neurotransmission in the brain. These findings show that intranasally applied Mn can impair spatial memory with significant changes in the tissue level and metabolism of monoamines in several brain regions. -- Highlights: ? Intranasal exposure to manganese in rats impairs spatial memory in the water maze. ? Regional changes in levels of neurotransmitters in the brain have been identified. ? Cognitive disorder correlates with modulation of 5-HT, NA and DA neurotransmission.

  11. Xyce Parallel Electronic Simulator Reference Guide Version 6.4

    SciTech Connect (OSTI)

    Keiter, Eric R.; Mei, Ting; Russo, Thomas V.; Schiek, Richard; Sholander, Peter E.; Thornquist, Heidi K.; Verley, Jason; Baur, David Gregory

    2015-12-01

    This document is a reference guide to the Xyce Parallel Electronic Simulator, and is a companion document to the Xyce Users' Guide [1] . The focus of this document is (to the extent possible) exhaustively list device parameters, solver options, parser options, and other usage details of Xyce . This document is not intended to be a tutorial. Users who are new to circuit simulation are better served by the Xyce Users' Guide [1] . Trademarks The information herein is subject to change without notice. Copyright c 2002-2015 Sandia Corporation. All rights reserved. Xyce TM Electronic Simulator and Xyce TM are trademarks of Sandia Corporation. Portions of the Xyce TM code are: Copyright c 2002, The Regents of the University of California. Produced at the Lawrence Livermore National Laboratory. Written by Alan Hindmarsh, Allan Taylor, Radu Serban. UCRL-CODE-2002-59 All rights reserved. Orcad, Orcad Capture, PSpice and Probe are registered trademarks of Cadence Design Systems, Inc. Microsoft, Windows and Windows 7 are registered trademarks of Microsoft Corporation. Medici, DaVinci and Taurus are registered trademarks of Synopsys Corporation. Amtec and TecPlot are trademarks of Amtec Engineering, Inc. Xyce 's expression library is based on that inside Spice 3F5 developed by the EECS Department at the University of California. The EKV3 MOSFET model was developed by the EKV Team of the Electronics Laboratory-TUC of the Technical University of Crete. All other trademarks are property of their respective owners. Contacts Bug Reports (Sandia only) http://joseki.sandia.gov/bugzilla http://charleston.sandia.gov/bugzilla World Wide Web http://xyce.sandia.gov http://charleston.sandia.gov/xyce (Sandia only) Email xyce@sandia.gov (outside Sandia) xyce-sandia@sandia.gov (Sandia only)

  12. Bromocriptine increased operant responding for high fat food but decreased chow intake in both obesity-prone and resistant rats

    SciTech Connect (OSTI)

    Thanos, P.K.; Wang, G.; Thanos, P.K.; Cho, J. Kim, R.; Michaelides, M.; Primeaux, S.; Bray, G.; Wang, G.-J.; Volkow, N.D.

    2010-10-27

    Dopamine (DA) and DAD{sub 2} receptors (D2R) have been implicated in obesity and are thought to be involved in the rewarding properties of food. Osborne-Mendel (OM) rats are susceptible to diet induced obesity (DIO) while S5B/P (S5B) rats are resistant when given a high-fat diet. Here we hypothesized that the two strains would differ in high-fat food self-administration (FSA) and that the D2R agonist bromocriptine (BC) would differently affect their behavior. Ad-libitum fed OM and S5B/P rats were tested in a FSA operant chamber and were trained to lever press for high-fat food pellets under a fixed-ratio (FR1) and a progressive ratio (PR) schedule. After sixteen days of PR sessions, rats were treated with three different doses of BC (1, 10 and 20 mg/kg). No significant differences were found between the two strains in the number of active lever presses. BC treatment (10 mg/kg and 20 mg/kg) increased the number of active lever presses (10 mg/kg having the strongest effect) whereas it decreased rat chow intake in the home cage with equivalent effects in both strains. These effects were not observed on the day of BC administration but on the day following its administration. Our results suggest that these two strains have similar motivation for procuring high fat food using this paradigm. BC increased operant responding for high-fat pellets but decreased chow intake in both strains, suggesting that D2R stimulation may have enhanced the motivational drive to procure the fatty food while correspondingly decreasing the intake of regular food. These findings suggest that susceptibility to dietary obesity (prior to the onset of obesity) may not affect operant motivation for a palatable high fat food and that differential susceptibility to obesity may be related to differential sensitivity to D2R stimulation.

  13. Good Practice Guide Waste Minimization/Pollution Prevention

    SciTech Connect (OSTI)

    J Dorsey

    1999-10-14

    This Good Practice Guide provides tools, information, and examples for promoting the implementation of pollution prevention during the design phases of U.S. Department of Energy (DOE) projects. It is one of several Guides for implementing DOE Order 430.1, Life-cycle Asset Management. DOE Order 430.1 provides requirements for DOE, in partnership with its contractors, to plan, acquire, operate, maintain, and dispose of physical assets. The goals of designing for pollution prevention are to minimize raw material consumption, energy consumption, waste generation, health and safety impacts, and ecological degradation over the entire life of the facility (EPA 1993a). Users of this Guide will learn to translate national policy and regulatory requirements for pollution prevention into action at the project level. The Guide was written to be applicable to all DOE projects, regardless of project size or design phase. Users are expected to interpret the Guide for their individual project's circumstances, applying a graded approach so that the effort is consistent with the anticipated waste generation and resource consumption of the physical asset. This Guide employs a combination of pollution prevention opportunity assessment (PPOA) methods and design for environment (DfE) philosophies. The PPOA process was primarily developed for existing products, processes, and facilities. The PPOA process has been modified in this Guide to address the circumstances of the DOE design process as delineated in DOE Order 430.1 and its associated Good Practice Guides. This modified form of the PPOA is termed the Pollution Prevention Design Assessment (P2DA). Information on current nationwide methods and successes in designing for the environment also have been reviewed and are integrated into this guidance.

  14. Structural basis of HIV-1 capsid recognition by PF74 and CPSF6

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Bhattacharya, Akash; Alam, Steven L.; Fricke, Thomas; Zadrozny, Kaneil; Sedzicki, Jaroslaw; Taylor, Alexander B.; Demeler, Borries; Pornillos, Owen; Ganser-Pornillos, Barbie K.; Diaz-Griffero, Felipe; et al

    2014-12-17

    Upon infection of susceptible cells by HIV-1, the conical capsid formed by ~250 hexamers and 12 pentamers of the CA protein is delivered to the cytoplasm. In this study, the capsid shields the RNA genome and proteins required for reverse transcription. In addition, the surface of the capsid mediates numerous host–virus interactions, which either promote infection or enable viral restriction by innate immune responses. In the intact capsid, there is an intermolecular interface between the N-terminal domain (NTD) of one subunit and the C-terminal domain (CTD) of the adjacent subunit within the same hexameric ring. The NTD–CTD interface is criticalmore » for capsid assembly, both as an architectural element of the CA hexamer and pentamer and as a mechanistic element for generating lattice curvature. Here we report biochemical experiments showing that PF-3450074 (PF74), a drug that inhibits HIV-1 infection, as well as host proteins cleavage and polyadenylation specific factor 6 (CPSF6) and nucleoporin 153 kDa (NUP153), bind to the CA hexamer with at least 10-fold higher affinities compared with nonassembled CA or isolated CA domains. The crystal structure of PF74 in complex with the CA hexamer reveals that PF74 binds in a preformed pocket encompassing the NTD–CTD interface, suggesting that the principal inhibitory target of PF74 is the assembled capsid. Likewise, CPSF6 binds in the same pocket. Given that the NTD–CTD interface is a specific molecular signature of assembled hexamers in the capsid, binding of NUP153 at this site suggests that key features of capsid architecture remain intact upon delivery of the preintegration complex to the nucleus.« less

  15. Structure and proteinprotein interactions of methanol dehydrogenase from Methylococcus capsulatus (Bath)

    SciTech Connect (OSTI)

    Culpepper, Megen A.; Rosenzweig, Amy C.

    2014-10-07

    In the initial steps of their metabolic pathway, methanotrophic bacteria oxidize methane to methanol with methane monooxygenases (MMOs) and methanol to formaldehyde with methanol dehydrogenases (MDHs). Several lines of evidence suggest that the membrane-bound or particulate MMO (pMMO) and MDH interact to form a metabolic supercomplex. To further investigate the possible existence of such a supercomplex, native MDH from Methylococcus capsulatus (Bath) has been purified and characterized by size exclusion chromatography with multi-angle light scattering and X-ray crystallography. M. capsulatus (Bath) MDH is primarily a dimer in solution, although an oligomeric species with a molecular mass of ~450560 kDa forms at higher protein concentrations. The 2.57 resolution crystal structure reveals an overall fold and ???? dimeric architecture similar to those of other MDH structures. In addition, biolayer interferometry studies demonstrate specific proteinprotein interactions between MDH and M. capsulatus (Bath) pMMO as well as between MDH and the truncated recombinant periplasmic domains of M. capsulatus (Bath) pMMO (spmoB). These interactions exhibit KD values of 833 409 nM and 9.0 7.7 ?M, respectively. The biochemical data combined with analysis of the crystal lattice interactions observed in the MDH structure suggest a model in which MDH and pMMO associate not as a discrete, stoichiometric complex but as a larger assembly scaffolded by the intracytoplasmic membranes.

  16. Development of taste sensor system for differentiation of Indonesian herbal medicines

    SciTech Connect (OSTI)

    Kaltsum, U.; Triyana, K. Siswanta, D.

    2014-09-25

    In Indonesia, herbal medicines are usually produced by small and medium enterprises which are relatively low in quality control. The purpose of this paper is to report that we have developed a taste sensor system with global selectivity, i.e., electronic tongue (e-tongue) for differentiation of Indonesian herbal medicines. The e-tongue was composed of five kinds of ion selective electrodes as working electrodes, data acquisition system, and pattern recognition system. Each ion selective electrode (ISE) was built by attaching lipid/polymer membrane. For this purpose, the five kinds of membranes were built by mixing lipid, plasticizer (nitrophenyl octyl ether/NPOE), polyvinyl chloride (PVC), and tetrahydrofuran (THF). In this study, we employed five kinds of lipid, namely oleic acid (OA), dioctyl phosphate (DOP), decyl alcohol (DA), dodecylamine (DDC), and trioctyl methyl ammonium chloride (TOMA). In this case, the membranes transform information of taste substances into electric signal. The five kinds of Indonesian herbal medicine were purchased from local supermarket in Yogyakarta, i.e., kunyit asam (made from turmeric and tamarind), beras kencur (made from rice and kencur), jahe wangi (made from ginger and fragrance), sirih wangi (made from betel leaf), and temulawak (made from Javanese ginger). Prior to detecting the taste from the Indonesian herbal medicine samples, each ion selective electrode was tested with five basic taste samples, i.e., for saltiness, sweetness, umami, bitterness, and sourness. All ISEs showed global selectivity to all samples. Furthermore, the array of ISEs showed specific response pattern to each Indonesian herbal medicine. For pattern recognition system, we employed principle component analysis (PCA). As a result, the e-tongue was able to differentiate five kinds of Indonesian herbal medicines, proven by the total variance of first and second principle components is about 93%. For the future, the e-tongue may be developed for quality control application in herbal medicine industries.

  17. Parametric Grid Information in the DOE Knowledge Base: Data Preparation, Storage, and Access

    SciTech Connect (OSTI)

    HIPP,JAMES R.; MOORE,SUSAN G.; MYERS,STEPHEN C.; SCHULTZ,CRAIG A.; SHEPHERD,ELLEN; YOUNG,CHRISTOPHER J.

    1999-10-01

    The parametric grid capability of the Knowledge Base provides an efficient, robust way to store and access interpolatable information which is needed to monitor the Comprehensive Nuclear Test Ban Treaty. To meet both the accuracy and performance requirements of operational monitoring systems, we use a new approach which combines the error estimation of kriging with the speed and robustness of Natural Neighbor Interpolation (NNI). The method involves three basic steps: data preparation (DP), data storage (DS), and data access (DA). The goal of data preparation is to process a set of raw data points to produce a sufficient basis for accurate NNI of value and error estimates in the Data Access step. This basis includes a set of nodes and their connectedness, collectively known as a tessellation, and the corresponding values and errors that map to each node, which we call surfaces. In many cases, the raw data point distribution is not sufficiently dense to guarantee accurate error estimates from the NNI, so the original data set must be densified using a newly developed interpolation technique known as Modified Bayesian Kriging. Once appropriate kriging parameters have been determined by variogram analysis, the optimum basis for NNI is determined in a process they call mesh refinement, which involves iterative kriging, new node insertion, and Delauny triangle smoothing. The process terminates when an NNI basis has been calculated which will fir the kriged values within a specified tolerance. In the data storage step, the tessellations and surfaces are stored in the Knowledge Base, currently in a binary flatfile format but perhaps in the future in a spatially-indexed database. Finally, in the data access step, a client application makes a request for an interpolated value, which triggers a data fetch from the Knowledge Base through the libKBI interface, a walking triangle search for the containing triangle, and finally the NNI interpolation.

  18. Structural basis of HIV-1 capsid recognition by PF74 and CPSF6

    SciTech Connect (OSTI)

    Bhattacharya, Akash; Alam, Steven L.; Fricke, Thomas; Zadrozny, Kaneil; Sedzicki, Jaroslaw; Taylor, Alexander B.; Demeler, Borries; Pornillos, Owen; Ganser-Pornillos, Barbie K.; Diaz-Griffero, Felipe; Ivanov, Dmitri N.; Yeager, Mark

    2014-12-17

    Upon infection of susceptible cells by HIV-1, the conical capsid formed by ~250 hexamers and 12 pentamers of the CA protein is delivered to the cytoplasm. In this study, the capsid shields the RNA genome and proteins required for reverse transcription. In addition, the surface of the capsid mediates numerous host–virus interactions, which either promote infection or enable viral restriction by innate immune responses. In the intact capsid, there is an intermolecular interface between the N-terminal domain (NTD) of one subunit and the C-terminal domain (CTD) of the adjacent subunit within the same hexameric ring. The NTD–CTD interface is critical for capsid assembly, both as an architectural element of the CA hexamer and pentamer and as a mechanistic element for generating lattice curvature. Here we report biochemical experiments showing that PF-3450074 (PF74), a drug that inhibits HIV-1 infection, as well as host proteins cleavage and polyadenylation specific factor 6 (CPSF6) and nucleoporin 153 kDa (NUP153), bind to the CA hexamer with at least 10-fold higher affinities compared with nonassembled CA or isolated CA domains. The crystal structure of PF74 in complex with the CA hexamer reveals that PF74 binds in a preformed pocket encompassing the NTD–CTD interface, suggesting that the principal inhibitory target of PF74 is the assembled capsid. Likewise, CPSF6 binds in the same pocket. Given that the NTD–CTD interface is a specific molecular signature of assembled hexamers in the capsid, binding of NUP153 at this site suggests that key features of capsid architecture remain intact upon delivery of the preintegration complex to the nucleus.

  19. Peptidome analysis of human skim milk in term and preterm milk

    SciTech Connect (OSTI)

    Wan, Jun; Cui, Xian-wei; Zhang, Jun; Fu, Zi-yi; Guo, Xi-rong; Institute of Pediatrics, Nanjing Medical University ; Sun, Li-Zhou; Ji, Chen-bo

    2013-08-16

    Highlights: A method was developed for preparation of peptide extracts from human milk. Analysis of the extracts by LCMS/MS resulted in the detection of 10003000 peptide-like features. 419 Peptides were identified by LCMS/MS from 34 proteins. Isotope dimethyl labeling analysis revealed 41 peptides differentially expressed. -- Abstract: The abundant proteins in human milk have been well characterized and are known to provide nutritional, protective, and developmental advantages to both term and preterm infants. Due to the difficulties associated with detection technology of the peptides, the expression of the peptides present in human milk is not known widely. In recent years, peptidome analysis has received increasing attention. In this report, the analysis of endogenous peptides in human milk was done by mass spectrometry. A method was also developed by our researchers, which can be used in the extraction of peptide from human milk. Analysis of the extracts by LCMS/MS resulted in the detection of 10003000 Da peptide-like features. Out of these, 419 peptides were identified by MS/MS. The identified peptides were found to originate from 34 proteins, of which several have been reported. Analysis of the peptides cleavage sites showed that the peptides are cleaved with regulations. This may reflect the protease activity and distribution in human body, and also represent the biological state of the tissue and provide a fresh source for biomarker discovery. Isotope dimethyl labeling analysis was also used to test the effects of premature delivery on milk protein composition in this study. Differences in peptides expression between breast milk in term milk (3841 weeks gestation) and preterm milk (2832 weeks gestation) were investigated in this study. 41 Peptides in these two groups were found expressed differently. 23 Peptides were present at higher levels in preterm milk, and 18 were present at higher levels in term milk.

  20. Fibroblast growth factor-2 up-regulates the expression of nestin through the RasRafERKSp1 signaling axis in C6 glioma cells

    SciTech Connect (OSTI)

    Chang, Kai-Wei; Huang, Yuan-Li; Wong, Zong-Ruei; Su, Peng-Han; Huang, Bu-Miin; Ju, Tsai-Kai; Technology Commons, College of Life Science, National Taiwan University, Taipei 106, Taiwan ; Yang, Hsi-Yuan

    2013-05-17

    Highlights: Nestin expression in C6 glioma cells is induced by FGF-2. Nestin expression is induced by FGF-2 via de novo RNA and protein synthesis. The FGFR inhibitor SU5402 blocks the FGF-2-induced nestin expression. The mRNA of FGFR1 and 3 are detected in C6 glioma cells. RasRafERKSp1 signaling pathway is responsibe for FGF-2-induced nestin expression. -- Abstract: Nestin is a 240-kDa intermediate filament protein expressed mainly in neural and myogenic stem cells. Although a substantial number of studies have focused on the expression of nestin during development of the central nervous system, little is known about the factors that induce and regulate its expression. Fibroblast growth factor-2 (FGF-2) is an effective mitogen and stimulates the proliferation and differentiation of a subset of nestin-expressing cells, including neural progenitor cells, glial precursor cells, and smooth muscle cells. To assess whether FGF-2 is a potent factor that induces the expression of nestin, C6 glioma cells were used. The results showed that nestin expression was up-regulated by FGF-2 via de novo RNA and protein synthesis. Our RT-PCR results showed that C6 glioma cells express FGFR1/3, and FGFRs is required for FGF-2-induced nestin expression. Further signaling analysis also revealed that FGF-2-induced nestin expression is mediated through FGFRMAPKERK signaling axis and the transcriptional factor Sp1. These findings provide new insight into the regulation of nestin in glial system and enable the further studies on the function of nestin in glial cells.

  1. Kinetics of ion-ion mutual neutralization: Halide anions with polyatomic cations

    SciTech Connect (OSTI)

    Shuman, Nicholas S.; Wiens, Justin P.; Miller, Thomas M.; Viggiano, Albert A.

    2014-06-14

    The binary mutual neutralization (MN) of a series of 17 cations (O{sub 2}{sup +}, NO{sup +}, NO{sub 2}{sup +}, CO{sup +}, CO{sub 2}{sup +}, Cl{sup +}, Cl{sub 2}{sup +}, SO{sub 2}{sup +}, CF{sub 3}{sup +}, C{sub 2}F{sub 5}{sup +}, NH{sub 3}{sup +}, H{sub 3}{sup +}, D{sub 3}{sup +}, H{sub 2}O{sup +}, H{sub 3}O{sup +}, ArH{sup +}, ArD{sup +}) with 3 halide anions (Cl{sup −}, Br{sup −}, I{sup −}) has been investigated in a flowing afterglow-Langmuir probe apparatus using the variable electron and neutral density attachment mass spectrometry technique. The MN rate constants of atom-atom reactions are dominated by the chemical nature of the system (i.e., the specific locations of curve crossings). As the number of atoms in the system increases, the MN rate constants become dominated instead by the physical nature of the system (e.g., the relative velocity of the reactants). For systems involving 4 or more atoms, the 300 K MN rate constants are well described by 2.7 × 10{sup −7} μ{sup −0.5}, where the reduced mass is in Da and the resulting rate constants in cm{sup 3} s{sup −1}. An upper limit to the MN rate constants appears well described by the complex potential model described by Hickman assuming a cross-section to neutralization of 11 000 Å{sup 2} at 300 K, equivalent to 3.5 × 10{sup −7} μ{sup −0.5}.

  2. Binding and Direct Electrochemistry of OmcA, an Outer-Membrane Cytochrome from an Iron Reducing Bacterium, with Oxide Electrodes: A Candidate Biofuel Cell System

    SciTech Connect (OSTI)

    Eggleston, Carrick M.; Voros, Janos; Shi, Liang; Lower, Brian H.; Droubay, Timothy C.; Colberg, Patricia J.

    2008-02-15

    Dissimilatory iron-reducing bacteria transfer electrons to solid ferric respiratory electron acceptors. Outer-membrane cytochromes expressed by these organisms are of interest in both microbial fuel cells and biofuel cells. We use optical waveguide lightmode spectroscopy (OWLS) to show that OmcA, an 85 kDa decaheme outer-membrane c-type cytochrome from Shewanella oneidensis MR-1, adsorbs to isostructural Al2O3 and Fe2O3 in similar amounts. Adsorption is ionic-strength and pH dependent (peak adsorption at pH 6.57.0). The thickness of the OmcA layer on Al2O3 at pH 7.0 [5.8 1.1 (2r) nm] from OWLS is similar, within error, to that observed using atomic force microscopy (4.8 2 nm). The highest adsorption density observed was 334 ng cm 2 (2.4 1012 molecules cm 2), corresponding to a monolayer or 9.9 nm diameter spheres or submonolayer coverage by smaller molecules. Direct electrochemistry of OmcA on Fe2O3 electrodes was observed using cyclic voltammetry, with cathodic peak potentials of 380 to 320 mV versus Ag/AgCl. Variations in the cathodic peak positions are speculatively attributed to redox-linked conformation change or changes in molecular orientation. OmcA can exchange electrons with ITO electrodes at higher current densities than with Fe2O3. Overall, OmcA can bind to and exchange electrons with several oxides, and thus its utility in fuel cells is not restricted to Fe2O3.

  3. A quantitative acoustic emission study on fracture processes in ceramics based on wavelet packet decomposition

    SciTech Connect (OSTI)

    Ning, J. G.; Chu, L.; Ren, H. L.

    2014-08-28

    We base a quantitative acoustic emission (AE) study on fracture processes in alumina ceramics on wavelet packet decomposition and AE source location. According to the frequency characteristics, as well as energy and ringdown counts of AE, the fracture process is divided into four stages: crack closure, nucleation, development, and critical failure. Each of the AE signals is decomposed by a 2-level wavelet package decomposition into four different (from-low-to-high) frequency bands (AA{sub 2}, AD{sub 2}, DA{sub 2}, and DD{sub 2}). The energy eigenvalues P{sub 0}, P{sub 1}, P{sub 2}, and P{sub 3} corresponding to these four frequency bands are calculated. By analyzing changes in P{sub 0} and P{sub 3} in the four stages, we determine the inverse relationship between AE frequency and the crack source size during ceramic fracture. AE signals with regard to crack nucleation can be expressed when P{sub 0} is less than 5 and P{sub 3} more than 60; whereas AE signals with regard to dangerous crack propagation can be expressed when more than 92% of P{sub 0} is greater than 4, and more than 95% of P{sub 3} is less than 45. Geiger location algorithm is used to locate AE sources and cracks in the sample. The results of this location algorithm are consistent with the positions of fractures in the sample when observed under a scanning electronic microscope; thus the locations of fractures located with Geiger's method can reflect the fracture process. The stage division by location results is in a good agreement with the division based on AE frequency characteristics. We find that both wavelet package decomposition and Geiger's AE source locations are suitable for the identification of the evolutionary process of cracks in alumina ceramics.

  4. P70S6K 1 regulation of angiogenesis through VEGF and HIF-1{alpha} expression

    SciTech Connect (OSTI)

    Bian, Chuan-Xiu; Shi, Zhumei; Meng, Qiao; Jiang, Yue; Liu, Ling-Zhi; Jiang, Bing-Hua; Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, Philadelphia, PA 19107

    2010-07-30

    Research highlights: {yields} P70S6K1 regulates VEGF expression; {yields} P70S6K1 induces transcriptional activation through HIF-1{alpha} binding site; {yields} P70S6K1 regulates HIF-1{alpha}, but not HIF-1{beta} protein expression; {yields} P70S6K1 mediates tumor growth and angiogenesis through HIF-1{alpha} and VEGF expression. -- Abstract: The 70 kDa ribosomal S6 kinase 1 (p70S6K1), a downstream target of phosphoinositide 3-kinase (PI3K) and ERK mitogen-activated protein kinase (MAPK), is an important regulator of cell cycle progression, and cell proliferation. Recent studies indicated an important role of p70S6K1 in PTEN-negative and AKT-overexpressing tumors. However, the mechanism of p70S6K1 in tumor angiogenesis remains to be elucidated. In this study, we specifically inhibited p70S6K1 activity in ovarian cancer cells using vector-based small interfering RNA (siRNA) against p70S6K1. We found that knockdown of p70S6K1 significantly decreased VEGF protein expression and VEGF transcriptional activation through the HIF-1{alpha} binding site at its enhancer region. The expression of p70S6K1 siRNA specifically inhibited HIF-1{alpha}, but not HIF-1{beta} protein expression. We also found that p70S6K1 down-regulation inhibited ovarian tumor growth and angiogenesis, and decreased cell proliferation and levels of VEGF and HIF-1{alpha} expression in tumor tissues. Our results suggest that p70S6K1 is required for tumor growth and angiogenesis through HIF-1{alpha} and VEGF expression, providing a molecular mechanism of human ovarian cancer mediated by p70S6K1 signaling.

  5. A COMPREHENSIVE SPECTROSCOPIC ANALYSIS OF DB WHITE DWARFS

    SciTech Connect (OSTI)

    Bergeron, P.; Wesemael, F.; Dufour, Pierre; Beauchamp, A.; Hunter, C.; Gianninas, A.; Limoges, M.-M.; Dufour, Patrick; Fontaine, G. [Departement de Physique, Universite de Montreal, C.P. 6128, Succ. Centre-Ville, Montreal, QC H3C 3J7 (Canada); Saffer, Rex A. [Strayer University, 234 Mall Boulevard, Suite G-50, King of Prussia, PA 19406 (United States); Ruiz, M. T. [Departamento de Astronomia, Universidad de Chile, Casilla 36-D, Santiago (Chile); Liebert, James, E-mail: bergeron@astro.umontreal.ca, E-mail: wesemael@astro.umontreal.ca, E-mail: gianninas@astro.umontreal.ca, E-mail: limoges@astro.umontreal.ca, E-mail: dufourpa@astro.umontreal.ca, E-mail: fontaine@astro.umontreal.ca, E-mail: alain.beauchamp@fti-ibis.com, E-mail: chris.hunter@yale.edu, E-mail: rex.saffer@strayer.edu, E-mail: mtruiz@das.uchile.cl [Steward Observatory, University of Arizona, Tucson, AZ 85721 (United States)

    2011-08-10

    We present a detailed analysis of 108 helium-line (DB) white dwarfs based on model atmosphere fits to high signal-to-noise optical spectroscopy. We derive a mean mass of 0.67 M{sub sun} for our sample, with a dispersion of only 0.09 M{sub sun}. White dwarfs also showing hydrogen lines, the DBA stars, comprise 44% of our sample, and their mass distribution appears similar to that of DB stars. As in our previous investigation, we find no evidence for the existence of low-mass (M < 0.5 M{sub sun}) DB white dwarfs. We derive a luminosity function based on a subset of DB white dwarfs identified in the Palomar-Green Survey. We show that 20% of all white dwarfs in the temperature range of interest are DB stars, although the fraction drops to half this value above T{sub eff} {approx} 20,000 K. We also show that the persistence of DB stars with no hydrogen features at low temperatures is difficult to reconcile with a scenario involving accretion from the interstellar medium, often invoked to account for the observed hydrogen abundances in DBA stars. We present evidence for the existence of two different evolutionary channels that produce DB white dwarfs: the standard model where DA stars are transformed into DB stars through the convective dilution of a thin hydrogen layer and a second channel where DB stars retain a helium atmosphere throughout their evolution. We finally demonstrate that the instability strip of pulsating V777 Her white dwarfs contains no non-variables, if the hydrogen content of these stars is properly accounted for.

  6. Observation of SOL Current Correlated with MHD Activity in NBI-heated DIII-D Tokamak Discharges

    SciTech Connect (OSTI)

    H. Takahashi; E.D. Fredrickson; M.J. Schaffer; M.E. Austin; T.E. Evans; L.L. Lao; J.G. Watkins

    2004-03-26

    This work investigates the potential roles played by the scrape-off-layer current (SOLC) in MHD activity of tokamak plasmas, including effects on stability. SOLCs are found during MHD activity that are: (1) slowly growing after a mode-locking-like event, (2) oscillating in the several kHz range and phase-locked with magnetic and electron temperature oscillations, (3) rapidly growing with a sub-ms time scale during a thermal collapse and a current quench, and (4) spiky in temporal behavior and correlated with spiky features in Da signals commonly identified with the edge localized mode (ELM). These SOLCs are found to be an integral part of the MHD activity, with a propensity to flow in a toroidally non-axisymmetric pattern and with magnitude potentially large enough to play a role in the MHD stability. Candidate mechanisms that can drive these SOLCs are identified: (a) toroidally non-axisymmetric thermoelectric potential, (b) electromotive force (EMF) from MHD activity, and (c) flux swing, both toroidal and poloidal, of the plasma column. An effect is found, stemming from the shear in the field line pitch angle, that mitigates the efficacy of a toroidally non-axisymmetric SOLC to generate a toroidally non-axisymmetric error field. Other potential magnetic consequences of the SOLC are identified: (i) its error field can introduce complications in feedback control schemes for stabilizing MHD activity and (ii) its toroidally non-axisymmetric field can be falsely identified as an axisymmetric field by the tokamak control logic and in equilibrium reconstruction. The radial profile of a SOLC observed during a quiescent discharge period is determined, and found to possess polarity reversals as a function of radial distance.

  7. Characterization of an acetyl xylan esterase from the anaerobic fungus Orpinomyces sp. strain PC-2

    SciTech Connect (OSTI)

    Blum, D.L.; Li, X.L.; Chen, H.; Ljungdahl, L.G.

    1999-09-01

    A 1,067-bp cDNA, designated axeA, coding for an acetyl xylan esterase (AxeA) was cloned from the anaerobic rumen fungus Orpinomyces sp. strain PC-2. The gene had an open reading frame of 939 bp encoding a polypeptide of 313 amino acid residues with a calculated mass of 34,845 Da. An active esterase using the original start codon of the cDNA was synthesized in Escherichia coli. Two active forms of the esterase were purified from recombinant E. coli cultures. The size difference of 8 amino acids was a result of cleavages at two different sites within the signal peptide. The enzyme released acetate from several acetylated substrates, including acetylated xylan. The activity toward acetylated xylan was tripled in the presence of recombinant xylanase A from the same fungus. Using p-nitrophenyl acetate as a substrate, the enzyme had a K{sub m} of 0.9 mM and a V{sub max} of 785 {micro}mol min{sup {minus}} mg{sup {minus}1}. It had temperature and pH optima of 30 C and 9.0, respectively. AxeA had 56% amino acid identity with BnaA, an acetyl xylan esterase of Neocallimastix patriciarum, but the Orpinomyces AxeA was devoid of a noncatalytic repeated peptide domain (NCRPD) found at the carboxy terminus of the Neocallimastix BnaA. The NCRPD found in many glycosyl hydrolases and esterases of anaerobic fungi has been postulated to function as a docking domain for cellulase-hemicellulase complexes, similar to the dockerin of the cellulosome of Clostridium thermocellum.

  8. Carbon monoxide inhalation increases microparticles causing vascular and CNS dysfunction

    SciTech Connect (OSTI)

    Xu, Jiajun; Yang, Ming; Kosterin, Paul; Salzberg, Brian M.; Milovanova, Tatyana N.; Bhopale, Veena M.; Thom, Stephen R.

    2013-12-01

    We hypothesized that circulating microparticles (MPs) play a role in pro-inflammatory effects associated with carbon monoxide (CO) inhalation. Mice exposed for 1 h to 100 ppm CO or more exhibit increases in circulating MPs derived from a variety of vascular cells as well as neutrophil activation. Tissue injury was quantified as 2000 kDa dextran leakage from vessels and as neutrophil sequestration in the brain and skeletal muscle; and central nervous system nerve dysfunction was documented as broadening of the neurohypophysial action potential (AP). Indices of injury occurred following exposures to 1000 ppm for 1 h or to 1000 ppm for 40 min followed by 3000 ppm for 20 min. MPs were implicated in causing injuries because infusing the surfactant MP lytic agent, polyethylene glycol telomere B (PEGtB) abrogated elevations in MPs, vascular leak, neutrophil sequestration and AP prolongation. These manifestations of tissue injury also did not occur in mice lacking myeloperoxidase. Vascular leakage and AP prolongation were produced in nave mice infused with MPs that had been obtained from CO poisoned mice, but this did not occur with MPs obtained from control mice. We conclude that CO poisoning triggers elevations of MPs that activate neutrophils which subsequently cause tissue injuries. - Highlights: Circulating microparticles (MPs) increase in mice exposed to 100 ppm CO or more. MPs are lysed by infusing the surfactant polyethylene glycol telomere B. CO-induced MPs cause neutrophil activation, vascular leak and CNS dysfunction. Similar tissue injuries do not arise with MPs obtained from air-exposed, control mice.

  9. Research in high energy physics. Progress report, 1 July 1993--30 June 1994

    SciTech Connect (OSTI)

    Rosen, J.; Block, M.; Buchholz, D.

    1994-07-01

    Progress on Task A centered around data analysis. E835 is now approved. It will extend E760 studies, exploring new charmonium states and featuring an upgraded detector system plus operation at 4--6 times higher luminosity. Results are given on E760 analysis. Task B has 10 papers that have either appeared in print, or have been prepared for publication. They break down into four categories; experimental physics, theoretical physics, and computer computational techniques. They are described here along with an exciting new experimental proposal to use Da{Phi}ne, the {Phi} factory that is being constructed at Frascati National Laboratory. Progress for Task C which includes participating in the D0 project at TeV I, and the photoproduction experiment, E687, at TeV II is given. While Northwestern is not participating in the top quark physics group at D0, they have been involved in the data analysis and the discussions that led to the limits on the top quark mass. Task D comprises the shared services for the Northwestern DOE contract. This includes the maintenance and operation of all computers within the HEP group. The projects supported by Task D during the past year are given. Task E progress was to resolve the apparent conflict between EMC, SMC, and SLAC results on nucleon structure functions and Bjorken sum rules. Task F covered research in hadronic decay of the tau, thermal field theory, plasma effects in astrophysics, and heavy quarkonium. Task G covers E665, a general purpose muon scattering experiment which can detect both the scattered muon and most charged and neutral hadrons produced in the forward region. The Northwest group has collaborated very closely in the past year with the Harvard group on analyses of structure functions and vector meson production in the 1991 data sample.

  10. Quantum Mechanical Calculations of Charge Effects on gating the KcsA channel

    SciTech Connect (OSTI)

    Kariev, Alisher M.; Znamenskiy, Vasiliy S.; Green, Michael E.

    2007-02-06

    The research described in this product was performed in part in the Environmental Molecular Sciences Laboratory, a national scientific user facility sponsored by the Department of Energy's Office of Biological and Environmental Research and located at Pacific Northwest National Laboratory. A series of ab initio (density functional) calculations were carried out on side chains of a set of amino acids, plus water, from the (intracellular) gating region of the KcsA K+ channel. Their atomic coordinates, except hydrogen, are known from X-ray structures [D.A. Doyle, J.M. Cabral, R.A. Pfuetzner, A. Kuo, J.M. Gulbis, S.L. Cohen, B.T. Chait, R. MacKinnon, The structure of the potassium channel: molecular basis of K+ conduction and selectivity, Science 280 (1998) 6977; R. MacKinnon, S.L. Cohen, A. Kuo, A. Lee, B.T. Chait, Structural conservation in prokaryotic and eukaryotic potassium channels, Science 280 (1998) 106109; Y. Jiang, A. Lee, J. Chen, M. Cadene, B.T. Chait, R. MacKinnon, The open pore conformation of potassium channels. Nature 417 (2001) 523526], as are the coordinates of some water oxygen atoms. The 1k4c structure is used for the starting coordinates. Quantum mechanical optimization, in spite of the starting configuration, places the atoms in positions much closer to the 1j95, more tightly closed, configuration. This state shows four water molecules forming a basket under the Q119 side chains, blocking the channel. When a hydrated K+ approaches this basket, the optimized system shows a strong set of hydrogen bonds with the K+ at defined positions, preventing further approach of the K+ to the basket. This optimized structure with hydrated K+ added shows an ice-like 12 molecule nanocrystal of water. If the water molecules exchange, unless they do it as a group, the channel will remain blocked. The basket itself appears to be very stable, although it is possible that the K+ with its hydrating water molecules may be more mobile, capable of withdrawing from the gate. It is also not surprising that water essentially freezes, or forms a kind of glue, in a nanometer space; this agrees with experimental results on a rather different, but similarly sized (nm dimensions) system [K.B. Jinesh, J.W.M. Frenken, Capillary condensation in atomic scale friction: how water acts like a glue, Phys. Rev. Lett. 96 (2006) 166103/14].

  11. Mammographic compression after breast conserving therapy: Controlling pressure instead of force

    SciTech Connect (OSTI)

    Groot, J. E. de Branderhorst, W.; Grimbergen, C. A.; Broeders, M. J. M.; Heeten, G. J. den; Department of Radiology, Academic Medical Center, P.O. Box 22660, 1100 DD Amsterdam

    2014-02-15

    Purpose: X-ray mammography is the primary tool for early detection of breast cancer and for follow-up after breast conserving therapy (BCT). BCT-treated breasts are smaller, less elastic, and more sensitive to pain. Instead of the current force-controlled approach of applying the same force to each breast, pressure-controlled protocols aim to improve standardization in terms of physiology by taking breast contact area and inelasticity into account. The purpose of this study is to estimate the potential for pressure protocols to reduce discomfort and pain, particularly the number of severe pain complaints for BCT-treated breasts. Methods: A prospective observational study including 58 women having one BCT-treated breast and one untreated nonsymptomatic breast, following our hospital's 18 decanewton (daN) compression protocol was performed. Breast thickness, applied force, contact area, mean pressure, breast volume, and inelasticity (mean E-modulus) were statistically compared between the within-women breast pairs, and data were used as predictors for severe pain, i.e., scores 7 and higher on an 11-point Numerical Rating Scale. Curve-fitting models were used to estimate how pressure-controlled protocols affect breast thickness, compression force, and pain experience. Results: BCT-treated breasts had on average 27% smaller contact areas, 30% lower elasticity, and 30% higher pain scores than untreated breasts (allp < 0.001). Contact area was the strongest predictor for severe pain (p < 0.01). Since BCT-treatment is associated with an average 0.36 dm{sup 2} decrease in contact area, as well as increased pain sensitivity, BCT-breasts had on average 5.3 times higher odds for severe pain than untreated breasts. Model estimations for a pressure-controlled protocol with a 10 kPa target pressure, which is below normal arterial pressure, suggest an average 26% (range 10%36%) reduction in pain score, and an average 77% (range 46%95%) reduction of the odds for severe pain. The estimated increase in thickness is +6.4% for BCT breasts. Conclusions: After BCT, women have hardly any choice in avoiding an annual follow-up mammogram. Model estimations show that a 10 kPa pressure-controlled protocol has the potential to reduce pain and severe pain particularly for these women. The results highly motivate conducting further research in larger subject groups.

  12. The interaction of high-speed turbulence with flames: Turbulent flame speed

    SciTech Connect (OSTI)

    Poludnenko, A.Y.; Oran, E.S. [Laboratory for Computational Physics and Fluid Dynamics, Naval Research Laboratory, Washington, DC 20375 (United States)

    2011-02-15

    Direct numerical simulations of the interaction of a premixed flame with driven, subsonic, homogeneous, isotropic, Kolmogorov-type turbulence in an unconfined system are used to study the mechanisms determining the turbulent flame speed, S{sub T}, in the thin reaction zone regime. High intensity turbulence is considered with the r.m.s. velocity 35 times the laminar flame speed, S{sub L}, resulting in the Damkoehler number Da=0.05. The simulations were performed with Athena-RFX, a massively parallel, fully compressible, high-order, dimensionally unsplit, reactive-flow code. A simplified reaction-diffusion model, based on the one-step Arrhenius kinetics, represents a stoichiometric H{sub 2}-air mixture under the assumption of the Lewis number Le=1. Global properties and the internal structure of the flame were analyzed in an earlier paper, which showed that this system represents turbulent combustion in the thin reaction zone regime. This paper demonstrates that: (1) The flame brush has a complex internal structure, in which the isosurfaces of higher fuel mass fractions are folded on progressively smaller scales. (2) Global properties of the turbulent flame are best represented by the structure of the region of peak reaction rate, which defines the flame surface. (3) In the thin reaction zone regime, S{sub T} is predominantly determined by the increase of the flame surface area, A{sub T}, caused by turbulence. (4) The observed increase of S{sub T} relative to S{sub L} exceeds the corresponding increase of A{sub T} relative to the surface area of the planar laminar flame, on average, by {approx}14%, varying from only a few percent to as high as {approx}30%. (5) This exaggerated response is the result of tight flame packing by turbulence, which causes frequent flame collisions and formation of regions of high flame curvature >or similar 1/{delta}{sub L}, or ''cusps,'' where {delta}{sub L} is the thermal width of the laminar flame. (6) The local flame speed in the cusps substantially exceeds its laminar value, which results in a disproportionately large contribution of cusps to S{sub T} compared with the flame surface area in them. (7) A criterion is established for transition to the regime significantly influenced by cusp formation. In particular, at Karlovitz numbers Ka >or similar 20, flame collisions provide an important mechanism controlling S{sub T}, in addition to the increase of A{sub T} by large-scale motions and the potential enhancement of diffusive transport by small-scale turbulence. (author)

  13. The Super-B Project Accelerator Status

    SciTech Connect (OSTI)

    Biagini, M.E.; Alesini, D.; Boni, R.; Boscolo, M.; Demma, T.; Drago, A.; Esposito, M.; Guiducci, S.; Marcellini, F.; Mazzitelli, G.; Preger, M.; Raimondi, P.; Sanelli, C.; Serio, M.; Stecchi, A.; Stella, A.; Tomassini, S.; Zobov, M.; Bertsche, K.; Brachmann, A.; Cai, Y.; /SLAC /Novosibirsk, IYF /Annecy, LAPP /LPSC, Grenoble /Orsay, LAL /Saclay /Pisa U. /CERN

    2011-08-17

    The SuperB project is an international effort aiming at building in Italy a very high luminosity e{sup +}e{sup -} (10{sup 36} cm{sup -2} sec{sup -1}) asymmetric collider at the Y(4S) energy in the CM. The accelerator design has been extensively studied and changed during the past year. The present design, based on the new collision scheme, with large Piwinski angle and the use of 'crab waist' sextupoles already successfully tested at the DA{Phi}NE {Phi}-Factory at LNF Frascati, provides larger flexibility, better dynamic aperture and spin manipulation sections in the Low Energy Ring (LER) for longitudinal polarization of the electron beam at the Interaction Point (IP). The Interaction Region (IR) has been further optimized in terms of apertures and reduced backgrounds in the detector. The injector complex design has been also updated. A summary of the project status will be presented in this paper. The SuperB collider can reach a peak luminosity of 10{sup 36} cm{sup -2} sec{sup -1} with beam currents and bunch lengths similar to those of the past and present e{sup +}e{sup -} Factories, through the use of smaller emittances and new scheme of crossing angle collision. The beams are stored in two rings at 6.7 GeV (HER) and 4.2 GeV (LER). Unique features of the project are the polarization of the electron beam in the LER and the possibility to decrease the energies for running at the {tau}/charm threshold. The option to reuse the PEP-II B-Factory (SLAC) hardware will allow reducing costs. The SuperB facility will require a big complex of civil infrastructure. The main construction, which will house the final part of the LINAC, the injection lines, the damping rings, and the storage rings, will be mainly underground. Two sites have been considered: the campus of Tor Vergata University near Frascati, and the INFN Frascati Laboratory. No decision has been made yet. A footprint of the possible SuperB layout on the LNF area is shown in Fig. 1.

  14. Coexistence of alternating ferromagnetic and antiferromagnetic intermolecular interactions in organic compounds. Synthesis, structure, thermal stability, and magnetic properties of 2,4-hexadiynylenedioxybis[2-(p,phenylene)-4,4,5,5-tetramethyl-4,5-dihydro-1H-imidazol-1-oxyl] diradical

    SciTech Connect (OSTI)

    Hernandez-Gasio, E.; Mas, M.; Molins, E.; Rovira, C.; Veciana, J.; Borras-Almenar, J.J.; Coronada, E.

    1994-12-01

    A crystalline phase of the title diradical has been prepared and characterized by X-ray diffraction, IR, UV-vis, and EPR spectroscopies, and magnetic susceptibility measurements. This phase belongs to the C2/c space group [a = 16.57(2) {angstrom}, b = 16.116(2) {angstrom}, c = 13.10(1) {angstrom}, {beta} = 123.05(4){degrees}, V = 2931(4) {angstrom}{sup 3}, Z = 4, d{sub calc} = 1.30 g cm{sup {minus}3}, T = 21{degrees}C, R{sub u} = 0.092, R{sub w} = 0.116]. The molecular structure of the diradical is characterized by an asymmetrical Z-shaped conformation. The most relevant features observed in the molecular packing are the large interdiacetylene separations - the shortest one is 8.285 {angstrom}-and the alternation in the characteristics of the intermolecular contacts between the radical side groups of the DA; which are jointed by hydrogen bonds between the oxygen atoms of NO groups and aromatic hydrogen atoms. On the basis of accepted structural criteria, this solid-state structure should not support a single-crystal topochemical polymerization and, accordingly, the UV-induced polymerization is not achieved. Thermal treatment, however, turns the crystals from blue to dark brown. Thermal analyses under nitrogen, performed with DSC and TGA techniques, reveal an explosive and complex decomposition, at temperatures higher than 90{degrees}C, with an evolution of gaseous NO (GC-MS) and a destruction of most of the radical centers of diradical molecules, as demonstrated by EPR and magnetic measurements. The study of the temperature dependence of the EPR signals of very diluted solutions of diradical 1 shows that it has a thermally modulated intramolecular exchange interaction due to the flexibility of the spacers joining the two radical centers and, furthermore, that when this diradical adopts a rigid conformation the two radical moieties are magnetically isolated (J{sup intra}/k {approximately} 0 K). 44 refs., 12 figs., 3 tabs.

  15. Exposure to tri-o-cresyl phosphate detected in jet airplane passengers

    SciTech Connect (OSTI)

    Liyasova, Mariya; Department of Environmental, Agricultural, and Occupational Health, University of Nebraska Medical Center, Omaha, NE ; Li, Bin; Schopfer, Lawrence M.; Nachon, Florian; Masson, Patrick; Eppley Institute, University of Nebraska Medical Center, Omaha, NE 68198-5950 ; Furlong, Clement E.; Lockridge, Oksana; Department of Environmental, Agricultural, and Occupational Health, University of Nebraska Medical Center, Omaha, NE

    2011-11-15

    The aircraft cabin and flight deck ventilation are supplied from partially compressed unfiltered bleed air directly from the engine. Worn or defective engine seals can result in the release of engine oil into the cabin air supply. Aircrew and passengers have complained of illness following such 'fume events'. Adverse health effects are hypothesized to result from exposure to tricresyl phosphate mixed esters, a chemical added to jet engine oil and hydraulic fluid for its anti-wear properties. Our goal was to develop a laboratory test for exposure to tricresyl phosphate. The assay was based on the fact that the active-site serine of butyrylcholinesterase reacts with the active metabolite of tri-o-cresyl phosphate, cresyl saligenin phosphate, to make a stable phosphorylated adduct with an added mass of 80 Da. No other organophosphorus agent makes this adduct in vivo on butyrylcholinesterase. Blood samples from jet airplane passengers were obtained 24-48 h after completing a flight. Butyrylcholinesterase was partially purified from 25 ml serum or plasma, digested with pepsin, enriched for phosphorylated peptides by binding to titanium oxide, and analyzed by mass spectrometry. Of 12 jet airplane passengers tested, 6 were positive for exposure to tri-o-cresyl phosphate that is, they had detectable amounts of the phosphorylated peptide FGEpSAGAAS. The level of exposure was very low. No more than 0.05 to 3% of plasma butyrylcholinesterase was modified. None of the subjects had toxic symptoms. Four of the positive subjects were retested 3 to 7 months following their last airplane trip and were found to be negative for phosphorylated butyrylcholinesterase. In conclusion, this is the first report of an assay that detects exposure to tri-o-cresyl phosphate in jet airplane travelers. -- Highlights: Black-Right-Pointing-Pointer Travel on jet airplanes is associated with an illness, aerotoxic syndrome. Black-Right-Pointing-Pointer A possible cause is exposure to tricresyl phosphate in engine lubricating oil. Black-Right-Pointing-Pointer A blood test for exposure to tri-o-cresyl phosphate is reported.

  16. Interesting features in the combined Galex and Sloan color diagrams of solar-like galactic populations

    SciTech Connect (OSTI)

    Smith, Myron A.; Shiao, Bernard; Bianchi, Luciana E-mail: shiao@stsci.edu

    2014-06-01

    We report on intriguing photometric properties of Galactic stars observed in the Galaxy Evolution Explorer (GALEX) satellite's far-UV (FUV) and near-UV (NUV) bandpasses, as well as from the ground-based Sloan Digital Sky Survey (SDSS) and the Kepler Input Catalog. The first property is that the (FUV NUV) color distribution of stars in the Kepler field consists of two well-separated peaks. A second and more perplexing property is that for stars with spectral types G or later the mean (FUV NUV) color becomes much bluer, contrary to expectation. Investigating this tendency further, we found in two samples of mid-F through K type stars that 17%-22% of them exhibit FUV excesses relative to their NUV fluxes and spectral types. A correction for FUV incompleteness of the FUV magnitude-limited star sample brings this ratio to 14%-18%. Nearly the same fractions are also discovered among members of the Kepler Eclipsing Binary Catalog and in the published list of Kepler Objects of Interest. These UV-excess ('UVe') colors are confirmed by the negative UV continuum slopes in GALEX spectra of members of the population. The SDSS spectra of some UVe stars exhibit metallic line weakening, especially in the blue. This suggests an enhanced contribution of UV flux relative to photospheric flux of a solar-type single star. We consider the possibility that the UV excesses originate from various types of hot stars, including white dwarf DA and sdB stars, binaries, and strong chromosphere stars that are young or in active binaries. The space density of compact stars is too low to explain the observed frequency of the UVe stars. Our model atmosphere-derived simulations of colors for binaries with main-sequence pairs with a hot secondary demonstrate that the color loci conflict with the observed sequence. As a preferred alternative we are left with the active chromospheres explanation, whether in active close binaries or young single stars, despite the expected paucity of young, chromospherically active stars in the field. We also address a third perplexing color property, namely, the presence of a prominent island of 'UV red' stars surrounded by 'UV blue' stars in the diagnostic (NUVg), (g i) color diagram. We find that the subpopulation composing this island is mainly horizontal branch stars. These objects do not exhibit UV excesses and therefore have UV colors typical for their spectral types. This subpopulation appears 'red' in the UV only because the stars' colors are not pulled to the blue by the inclusion of UVe stars.

  17. Xyce Parallel Electronic Simulator Users Guide Version 6.2.

    SciTech Connect (OSTI)

    Keiter, Eric R.; Mei, Ting; Russo, Thomas V.; Schiek, Richard; Sholander, Peter E.; Thornquist, Heidi K.; Verley, Jason; Baur, David Gregory

    2014-09-01

    This manual describes the use of the Xyce Parallel Electronic Simulator. Xyce has been de- signed as a SPICE-compatible, high-performance analog circuit simulator, and has been written to support the simulation needs of the Sandia National Laboratories electrical designers. This development has focused on improving capability over the current state-of-the-art in the following areas: Capability to solve extremely large circuit problems by supporting large-scale parallel com- puting platforms (up to thousands of processors). This includes support for most popular parallel and serial computers. A differential-algebraic-equation (DAE) formulation, which better isolates the device model package from solver algorithms. This allows one to develop new types of analysis without requiring the implementation of analysis-specific device models. Device models that are specifically tailored to meet Sandia's needs, including some radiation- aware devices (for Sandia users only). Object-oriented code design and implementation using modern coding practices. Xyce is a parallel code in the most general sense of the phrase -- a message passing parallel implementation -- which allows it to run efficiently a wide range of computing platforms. These include serial, shared-memory and distributed-memory parallel platforms. Attention has been paid to the specific nature of circuit-simulation problems to ensure that optimal parallel efficiency is achieved as the number of processors grows. Trademarks The information herein is subject to change without notice. Copyright c 2002-2014 Sandia Corporation. All rights reserved. Xyce TM Electronic Simulator and Xyce TM are trademarks of Sandia Corporation. Portions of the Xyce TM code are: Copyright c 2002, The Regents of the University of California. Produced at the Lawrence Livermore National Laboratory. Written by Alan Hindmarsh, Allan Taylor, Radu Serban. UCRL-CODE-2002-59 All rights reserved. Orcad, Orcad Capture, PSpice and Probe are registered trademarks of Cadence Design Systems, Inc. Microsoft, Windows and Windows 7 are registered trademarks of Microsoft Corporation. Medici, DaVinci and Taurus are registered trademarks of Synopsys Corporation. Amtec and TecPlot are trademarks of Amtec Engineering, Inc. Xyce 's expression library is based on that inside Spice 3F5 developed by the EECS Department at the University of California. The EKV3 MOSFET model was developed by the EKV Team of the Electronics Laboratory-TUC of the Technical University of Crete. All other trademarks are property of their respective owners. Contacts Bug Reports (Sandia only) http://joseki.sandia.gov/bugzilla http://charleston.sandia.gov/bugzilla World Wide Web http://xyce.sandia.gov http://charleston.sandia.gov/xyce (Sandia only) Email xyce%40sandia.gov (outside Sandia) xyce-sandia%40sandia.gov (Sandia only)

  18. The International Safeguards Technology Base: How is the Patient Doing? An Exploration of Effective Metrics

    SciTech Connect (OSTI)

    Schanfein, Mark; Gouveia, Fernando; Crawford, Cary E.; Pickett, Chris J.; Jay, Jeffrey

    2010-07-15

    The term “Technology Base” is commonly used but what does it mean? Is there a common understanding of the components that comprise a technology base? Does a formal process exist to assess the health of a given technology base? These are important questions the relevance of which is even more pressing given the USDOE/NNSA initiatives to strengthen the safeguards technology base through investments in research & development and human capital development. Accordingly, the authors will establish a high-level framework to define and understand what comprises a technology base. Potential goal-driven metrics to assess the health of a technology base will also be explored, such as linear demographics and resource availability, in the hope that they can be used to better understand and improve the health of the U.S. safeguards technology base. Finally, through the identification of such metrics, the authors will offer suggestions and highlight choices for addressing potential shortfalls. Introduction The U.S. safeguards technology base got its start almost half a century ago in the nuclear weapons program of the U.S. Department of Energy/National Nuclear Security Administration (DOE/NNSA) and their predecessors: AEC & ERDA. Due to nuclear materials’ strategic importance and value, and the risk associated with the public’s and worker’s health and the potential for theft, significant investments were made to develop techniques to measure nuclear materials using both destructive assay (DA) and non-destructive assay (NDA). Major investment within the U.S. DOE Domestic Safeguards Program continued over the next three decades, resulting in continuous improvements in the state-of-the-art of these techniques. This was particularly true in the area of NDA with its ability to use gamma rays, neutrons, and heat to identify and quantify nuclear materials without the need to take direct samples of the material. Most of these techniques were commercialized and transferred to industry, opening their applications to the nuclear industry worldwide and to the International Atomic Energy Agency (IAEA).

  19. The ionic charge of Copper-64 complexes conjugated to an engineered antibody effects biodistribution

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Dearling, Jason L. J.; Smith, Suzanne V.; Paterson, Brett M.; Akurathi, Vamisidhar; Betanzos-Lara, Soledad; Treves, S. Ted; Voss, Stephan D.; White, Jonathan M.; Huston, James S.; Donnelly, Paul S.; et al

    2015-04-15

    The development of biomolecules as imaging probes requires radiolabeling methods that do not significantly influence their biodistribution. Sarcophagine (Sar) chelators form extremely stable complexes with copper, and are therefore a promising option for labeling proteins with ⁶⁴Cu. However, initial studies using the first-generation sarcophagine bifunctional chelator SarAr to label the engineered antibody fragment ch14.18-ΔCH2 (MW 120 kDa) with ⁶⁴Cu showed high tracer retention in the kidneys,(>38% injected dose per gram (ID/g) 48 h post-injection), presumably because the high local positive charge on the CuII-SarAr moiety resulted in increased binding of the labeled protein to the negatively charged basal cells ofmore » the glomerulus. To test this hypothesis, ch14.18-ΔCH2 was conjugated with a series of Sar derivatives of decreasing positive charge and three commonly used macrocyclic polyaza polycarboxylate (PAC) BFCs. The immunoconjugates were labeled with ⁶⁴Cu and injected into mice, and PET/CT images were obtained at 24 and 48 h post injection (p.i.). At 48 h p.i., ex vivo biodistribution was carried out. In addition, to demonstrate the potential of metastasis detection using ⁶⁴Cu-labeled ch14.18-ΔCH2, a preclinical imaging study of intrahepatic neuroblastoma tumors was performed carried out. Reducing the positive charge on the Sar chelators decreased kidney uptake of Cu-labeled ch14.18-ΔCH2 by more than 6-fold, from >45 ID/g to <6% ID/g, while the uptake in most other tissues, including liver, was relatively unchanged. However, despite this dramatic decrease, the renal uptake of the PAC BFCs was generally lower than that of the Sar derivatives, as was the liver uptake. Uptake of ⁶⁴Cu-labeled ch14.18-ΔCH2 in neuroblastoma hepatic metastases was detected using PET.« less

  20. Molecular cloning and functional characterization of a rainbow trout liver Oatp

    SciTech Connect (OSTI)

    Steiner, Konstanze; Hagenbuch, Bruno; Dietrich, Daniel R.

    2014-11-01

    Cyanobacterial blooms have an impact on the aquatic ecosystem due to the production of toxins (e.g. microcystins, MCs), which constrain fish health or even cause fish death. However the toxicokinetics of the most abundant toxin, microcystin-LR (MC-LR), are not yet fully understood. To investigate the uptake mechanism, the novel Oatp1d1 in rainbow trout (rtOatp1d1) was cloned, identified and characterized. The cDNA isolated from a clone library consisted of 2772 bp containing a 2115 bp open reading frame coding for a 705 aa protein with an approximate molecular mass of 80 kDa. This fish specific transporter belongs to the OATP1 family and has most likely evolved from a common ancestor of OATP1C1. Real time PCR analysis showed that rtOatp1d1 is predominantly expressed in the liver, followed by the brain while expression in other organs was not detectable. Transient transfection in HEK293 cells was used for further characterization. Like its human homologues OATP1A1, OATP1B1 and OATP1B3, rtOatp1d1 displayed multi-specific transport including endogenous and xenobiotic substrates. Kinetic analyses revealed a K{sub m} value of 13.9 μM and 13.4 μM for estrone-3-sulfate and methotrexate, respectively and a rather low affinity for taurocholate with a K{sub m} value of 103 μM. Furthermore, it was confirmed that rtOatp1d1 is a MC-LR transporter and therefore most likely plays a key role in the susceptibility of rainbow trout to MC intoxications. - Highlights: • A new Oatp1d1 in rainbow trout (rtOatp1d1) was cloned, identified and characterized. • rtOatp1d1 is predominantly expressed in the liver. • rtOatp1d1 displays multi-specific transport of endogenous and xenobiotic substrates. • rtOatp1d1 is a homologue of the OATP1A1, OATP1B1 and OATP1B3. • rtOatp1d1 is a microcystin (MC) transporter.