National Library of Energy BETA

Sample records for d-3 ement cd-4

  1. DEPARTlIiIENT OF ENERGY EERE PROJECT MAN AG EMENT CENTER NEPA...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    lAUIl) u.s. DEPARTlIiIENT OF ENERGY EERE PROJECT MAN AG EMENT CENTER NEPA DETERMINATION RECIPIENT:Ocean Engineering and Energy Systems Intemational, Inc. (OCEES) Page lof3 STATE: ...

  2. DOE CD-4 Review of the MINERvA Project

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    DOE CD-4 Review of the MINERvA Project Main INjector ExpeRiment v-A May 12, 2010 | Overview | CD-4 Documentation| Other Documents| Overview of Charge and Experiment MINERvA Overview (2.2 MB pdf) Review Agenda (all times EDT) (pdf) Review Charge (pdf) Review Presentation by MINERvA Project (DRAFT until the week of May 12) (docdb page) Required CD-4 Documentation CD-4 Document Checklist (pdf) Technical Requirements Completion Document (pdf) Quality Assurance Plan (pdf) Safety Documentation Hazard

  3. Preliminary Safety Design RM

    Office of Environmental Management (EM)

    Preliminary Safety Design Review Module March 2010 CD-0 O 0 OFFICE OF Pr C CD-1 F ENVIRO ... (CD) Ap CD March 2010 L MANAGE n (SRP) y Design e pplicability D-3 EMENT CD-4 Post Ope ...

  4. Readiness Review RM

    Office of Environmental Management (EM)

    March 2010 CD-0 O 0 OFFICE OF C CD-1 F ENVIRO Standard R Readin Rev Critical Decis CD-2 M ... D-3 EMENT CD-4 Post Ope eration Standard Review Plan, 2 nd Edition, March 2010 i ...

  5. C-340 Complex D&D Final Lessons Learned (Post CD-4), Environmental...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    C-340 Complex D&D Final Lessons Learned (Post CD-4), Environmental Management Cleanup, Sep 2011 C-340 Complex D&D Final Lessons Learned (Post CD-4), Environmental Management ...

  6. Maturation Pathway from Germline to Broad HIV-1 Neutralizer of a CD4-Mimic Antibody

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Bonsignori, Mattia; Zhou, Tongqing; Sheng, Zizhang; Chen, Lei; Gao, Feng; Joyce, M.  Gordon; Ozorowski, Gabriel; Chuang, Gwo-Yu; Schramm, Chaim A.; Wiehe, Kevin; et al

    2016-04-01

    Here, we report that antibodies with ontogenies from VH1-2 or VH1-46-germline genes dominate the broadly neutralizing response against the CD4-binding site (CD4bs) on HIV-1. We define with longitudinal sampling from time-of-infection the development of a VH1-46-derived antibody lineage that matured to neutralize 90% of HIV-1 isolates. Structures of lineage antibodies CH235 (week 41 from time-of-infection, 18% breadth), CH235.9 (week 152, 77%), and CH235.12 (week 323, 90%) demonstrated the maturing epitope to focus on the conformationally invariant portion of the CD4bs. Similarities between CH235 lineage and five unrelated CD4bs lineages in epitope focusing, length-of-time to develop breadth, and extraordinary level ofmore » somatic hypermutation suggested commonalities in maturation among all CD4bs antibodies. Fortunately, the required CH235-lineage hypermutation appeared substantially guided by the intrinsic mutability of the VH1-46 gene, which closely resembled VH1-2. Lastly, we integrated our CH235-lineage findings with a second broadly neutralizing lineage and HIV-1 co-evolution to suggest a vaccination strategy for inducing both lineages.« less

  7. Strain-Specific V3 and CD4 Binding Site Autologous HIV-1 Neutralizing Antibodies Select Neutralization-Resistant Viruses

    SciTech Connect (OSTI)

    Moody, M.  Anthony; Gao, Feng; Gurley, Thaddeus  C.; Amos, Joshua  D.; Kumar, Amit; Hora, Bhavna; Marshall, Dawn  J.; Whitesides, John  F.; Xia, Shi-Mao; Parks, Robert; Lloyd, Krissey  E.; Hwang, Kwan-Ki; Lu, Xiaozhi; Bonsignori, Mattia; Finzi, Andrés; Vandergrift, Nathan  A.; Alam, S.  Munir; Ferrari, Guido; Shen, Xiaoying; Tomaras, Georgia  D.; Kamanga, Gift; Cohen, Myron  S.; Sam, Noel  E.; Kapiga, Saidi; Gray, Elin S.; Tumba, Nancy  L.; Morris, Lynn; Zolla-Pazner, Susan; Gorny, Miroslaw  K.; Mascola, John  R.; Hahn, Beatrice H.; Shaw, George  M.; Sodroski, Joseph  G.; Liao, Hua-Xin; Montefiori, David C.; Hraber, Peter T.; Korber, Bette T.; Haynes, Barton F.

    2015-09-09

    The third variable (V3) loop and the CD4 binding site (CD4bs) of the viral envelope are frequently targeted by neutralizing antibodies (nAbs) in HIV-1-infected individuals. In chronic infection, virus escape mutants repopulate the plasma and V3 and CD4bs nAbs emerge that can neutralize heterologous tier 1 easy-to-neutralize, but not tier 2 difficult-to-neutralize HIV-1 isolates. However, neutralization sensitivity of autologous plasma viruses to this type of nAb response has not been studied. We describe the development and evolution in vivo of antibodies distinguished by their target specificity for V3and CD4bs epitopes on autologous tier 2 viruses but not on heterologous tier 2 viruses. A surprisingly high fraction of autologous circulating viruses was sensitive to these antibodies. These findings demonstrate a role for V3 and CD4bs antibodies in constraining the native envelope trimer in vivo to a neutralization-resistant phenotype, explaining why HIV-1 transmission generally occurs by tier 2 neutralization-resistant viruses.

  8. Strain-Specific V3 and CD4 Binding Site Autologous HIV-1 Neutralizing Antibodies Select Neutralization-Resistant Viruses

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Moody, M.  Anthony; Gao, Feng; Gurley, Thaddeus  C.; Amos, Joshua  D.; Kumar, Amit; Hora, Bhavna; Marshall, Dawn  J.; Whitesides, John  F.; Xia, Shi-Mao; Parks, Robert; et al

    2015-09-09

    The third variable (V3) loop and the CD4 binding site (CD4bs) of the viral envelope are frequently targeted by neutralizing antibodies (nAbs) in HIV-1-infected individuals. In chronic infection, virus escape mutants repopulate the plasma and V3 and CD4bs nAbs emerge that can neutralize heterologous tier 1 easy-to-neutralize, but not tier 2 difficult-to-neutralize HIV-1 isolates. However, neutralization sensitivity of autologous plasma viruses to this type of nAb response has not been studied. We describe the development and evolution in vivo of antibodies distinguished by their target specificity for V3and CD4bs epitopes on autologous tier 2 viruses but not on heterologous tiermore » 2 viruses. A surprisingly high fraction of autologous circulating viruses was sensitive to these antibodies. These findings demonstrate a role for V3 and CD4bs antibodies in constraining the native envelope trimer in vivo to a neutralization-resistant phenotype, explaining why HIV-1 transmission generally occurs by tier 2 neutralization-resistant viruses.« less

  9. Automated D/3 to Visio Analog Diagrams

    Energy Science and Technology Software Center (OSTI)

    2000-08-10

    ADVAD1 reads an ASCII file containing the D/3 DCS MDL input for analog points for a D/3 continuous database. It uses the information in the files to create a series of Visio files representing the structure of each analog chain, one drawing per Visio file. The actual drawing function is performed by Visio (requires Visio version 4.5+). The user can configure the program to select which fields in the database are shown on the diagrammore » and how the information is to be presented. This gives a visual representation of the structure of the analog chains, showing selected fields in a consistent manner. Updating documentation can be done easily and the automated approach eliminates human error in the cadding process. The program can also create the drawings far faster than a human operator is capable, able to create approximately 270 typical diagrams in about 8 minutes on a Pentium II 400 MHz PC. The program allows for multiple option sets to be saved to provide different settings (i.e., different fields, different field presentations, and /or different diagram layouts) for various scenarios or facilities on one workstation. Option sets may be exported from the Windows registry to allow duplication of settings on another workstation.« less

  10. Id1 expression promotes peripheral CD4{sup +} T cell proliferation and survival upon TCR activation without co-stimulation

    SciTech Connect (OSTI)

    Liu, Chen; Jin, Rong; Wang, Hong-Cheng; Tang, Hui; Liu, Yuan-Feng; Qian, Xiao-Ping; Sun, Xiu-Yuan; Ge, Qing; Sun, Xiao-Hong; Zhang, Yu

    2013-06-21

    Highlights: •Id1 expression enables naïve T cell proliferation without anti-CD28 co-stimulation. •Id1 expression facilitates T cells survival when stimulated with anti-CD3. •Elevation of IL-2 production by Id1 contributes increased proliferation and survival. •Id1 potentiates NF-κB activation by anti-CD3 stimulation. -- Abstract: Although the role of E proteins in the thymocyte development is well documented, much less is known about their function in peripheral T cells. Here we demonstrated that CD4 promoter-driven transgenic expression of Id1, a naturally occurring dominant-negative inhibitor of E proteins, can substitute for the co-stimulatory signal delivered by CD28 to facilitate the proliferation and survival of naïve CD4{sup +} cells upon anti-CD3 stimulation. We next discovered that IL-2 production and NF-κB activity after anti-CD3 stimulation were significantly elevated in Id1-expressing cells, which may be, at least in part, responsible for the augmentation of their proliferation and survival. Taken together, results from this study suggest an important role of E and Id proteins in peripheral T cell activation. The ability of Id proteins to by-pass co-stimulatory signals to enable T cell activation has significant implications in regulating T cell immunity.

  11. Gastrodin stimulates anticancer immune response and represses transplanted H22 hepatic ascitic tumor cell growth: Involvement of NF-?B signaling activation in CD4 + T cells

    SciTech Connect (OSTI)

    Shu, Guangwen; Yang, Tianming; Wang, Chaoyuan; Su, Hanwen; Xiang, Meixian

    2013-06-15

    Gastrodia elata Blume (G. elata) is a famous restorative food in East Asia. It can be used as an auxiliary reagent in hepatocellular carcinoma (HCC) treatment. Previous studies unveiled that G. elata exhibited immunomodulatory activities. To explore the active ingredients contributing to its immunomodulatory activities, gastrodin, vanillin, and parishin B were purified from G. elata and their anti-HCC effects were assessed in vivo. Among these compounds, only gastrodin was capable of repressing transplanted H22 ascitic hepatic tumor cell growth in vivo with low toxicity. Further investigations were designed to explore the effects of gastrodin on the immune system of tumor-bearing mice and potential molecular mechanisms underlying these effects. Our data showed that gastrodin ameliorated tumor cell transplantation-induced activation of endogenous pro-apoptotic pathway in CD4 + T cells and abnormalities in serum cytokine profiles in host animals. These events enhanced cytotoxic activities of natural killer and CD8 + T cells against H22 hepatic cancer cells. Gastrodin administration specifically upregulated mRNA levels of several nuclear factor ?B (NF-?B) responsive genes in CD4 + T cells but not in CD8 + T cells. Chromatin immunoprecipitation assay showed that gastrodin increased the association of NF-?B p65 subunit to the promoter regions of IL-2 and Bcl-2 encoding genes in CD4 + T cells. Our investigations demonstrated that gastrodin is the main active ingredient contributing to the anticancer immunomodulatory properties of G. elata. Promoting NF-?B-mediated gene transcription in CD4 + T cells is implicated in its immunomodulatory activity. - Highlights: Gastrodin stimulates anticancer immune response. Gastrodin represses tumor transplantation-induced CD4 + T cell apoptosis. Gastrodin activates NF-?B activity in CD4 + T cells.

  12. Quality Assurance for Critical Decision Reviews RM

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Quality Assurance for Critical Decision Reviews Module March 2010 CD-0 O 0 OFFICE OF Q C CD-1 F ENVIRO Standard R Quality A Rev Critical Decis CD-2 M ONMENTAL Review Plan Assuranc view Module sion (CD) Ap CD March 2010 L MANAGE n (SRP) e (QA) e pplicability D-3 EMENT CD-4 Post Ope eration Standard Review Plan, 2 nd Edition, March 2010 i FOREWORD The Standard Review Plan (SRP) 1 provides a consistent, predictable corporate review framework to ensure that issues and risks that could challenge the

  13. Interactive initialization of 2D/3D rigid registration

    SciTech Connect (OSTI)

    Gong, Ren Hui; Gler, zgr; Krkloglu, Mustafa; Lovejoy, John; Yaniv, Ziv

    2013-12-15

    Purpose: Registration is one of the key technical components in an image-guided navigation system. A large number of 2D/3D registration algorithms have been previously proposed, but have not been able to transition into clinical practice. The authors identify the primary reason for the lack of adoption with the prerequisite for a sufficiently accurate initial transformation, mean target registration error of about 10 mm or less. In this paper, the authors present two interactive initialization approaches that provide the desired accuracy for x-ray/MR and x-ray/CT registration in the operating room setting. Methods: The authors have developed two interactive registration methods based on visual alignment of a preoperative image, MR, or CT to intraoperative x-rays. In the first approach, the operator uses a gesture based interface to align a volume rendering of the preoperative image to multiple x-rays. The second approach uses a tracked tool available as part of a navigation system. Preoperatively, a virtual replica of the tool is positioned next to the anatomical structures visible in the volumetric data. Intraoperatively, the physical tool is positioned in a similar manner and subsequently used to align a volume rendering to the x-ray images using an augmented reality (AR) approach. Both methods were assessed using three publicly available reference data sets for 2D/3D registration evaluation. Results: In the authors' experiments, the authors show that for x-ray/MR registration, the gesture based method resulted in a mean target registration error (mTRE) of 9.3 5.0 mm with an average interaction time of 146.3 73.0 s, and the AR-based method had mTREs of 7.2 3.2 mm with interaction times of 44 32 s. For x-ray/CT registration, the gesture based method resulted in a mTRE of 7.4 5.0 mm with an average interaction time of 132.1 66.4 s, and the AR-based method had mTREs of 8.3 5.0 mm with interaction times of 58 52 s. Conclusions: Based on the authors

  14. The role of intrahepatic CD3 +/CD4 −/CD8 − double negative T (DN T) cells in enhanced acetaminophen toxicity

    SciTech Connect (OSTI)

    Getachew, Yonas; Cusimano, Frank A.; James, Laura P.; Thiele, Dwain L.

    2014-10-15

    The role of the immune system, specifically NK, NKT and CD3 cells, in acetaminophen (APAP) induced liver injury remains inconsistently defined. In the present study, wild type (C57BL/6J) mice and granzyme B deficient (GrB −/−) mice were treated with acetaminophen to assess the role of the immune system in acute liver injury. Doses of acetaminophen that induced sub lethal liver injury in wild type mice unexpectedly produced fatal hepatotoxicity in granzyme B deficient (GrB −/−) mice. Analysis revealed that GrB −/− mice had an increased population of intrahepatic CD3 (+), CD4 (−), and CD8 (−) lymphocytes expressing the CD69 activation marker and Fas ligand. Depletion of these cells in the GrB −/− and wild type mice made them less susceptible to APAP injury, while depletion of NK1.1 (+) cells or both CD4 (+) and CD8 (+) T cells failed to provide the same hepatoprotection. Transfer of the GrB −/− IHLs further exacerbated liver injury and increased mortality in wild type mice but not in LRP/LPR mice, lacking fas expression. Conclusions: Acetaminophen toxicity is enhanced by the presence of activated, FasL expressing intrahepatic CD3 (+), CD4 (−), CD8 (−), NK1.1 (−) T cells. Depletion of these cells from GrB −/− mice and wild type mice greatly reduces mortality and improves the course of liver injury recovery. - Highlights: • Intrahepatic lymphocytes (IHLs) from GrB −/− mice harbor activated DNT cells. • IHLs from GrB −/− mice exhibit enhanced Fas ligand expression. • Acetaminophen toxicity is enhanced by activated, FasL expressing DNT cells.

  15. 2D/3D registration algorithm for lung brachytherapy

    SciTech Connect (OSTI)

    Zvonarev, P. S.; Farrell, T. J.; Hunter, R.; Wierzbicki, M.; Hayward, J. E.; Sur, R. K.

    2013-02-15

    Purpose: A 2D/3D registration algorithm is proposed for registering orthogonal x-ray images with a diagnostic CT volume for high dose rate (HDR) lung brachytherapy. Methods: The algorithm utilizes a rigid registration model based on a pixel/voxel intensity matching approach. To achieve accurate registration, a robust similarity measure combining normalized mutual information, image gradient, and intensity difference was developed. The algorithm was validated using a simple body and anthropomorphic phantoms. Transfer catheters were placed inside the phantoms to simulate the unique image features observed during treatment. The algorithm sensitivity to various degrees of initial misregistration and to the presence of foreign objects, such as ECG leads, was evaluated. Results: The mean registration error was 2.2 and 1.9 mm for the simple body and anthropomorphic phantoms, respectively. The error was comparable to the interoperator catheter digitization error of 1.6 mm. Preliminary analysis of data acquired from four patients indicated a mean registration error of 4.2 mm. Conclusions: Results obtained using the proposed algorithm are clinically acceptable especially considering the complications normally encountered when imaging during lung HDR brachytherapy.

  16. Visualization of the human CD4{sup +} T-cell response in humanized HLA-DR4-expressing NOD/Shi-scid/γc{sup null} (NOG) mice by retrogenic expression of the human TCR gene

    SciTech Connect (OSTI)

    Takahashi, Takeshi Katano, Ikumi; Ito, Ryoji; Ito, Mamoru

    2015-01-02

    Highlights: • β-Lactoglobulin (BLG) specific TCR genes were introduced to human HSC by retrovirus. • Human HSC with BLG-specific TCR were transplanted into NOG-HLA-DR4 I-A{sup −/−} mice. • BLG-specific TCR induced positive selection of thymocytes. • BLG-specific TCR positive CD4{sup +} T cells mediated immune responses in humanized mice. - Abstract: The development of severe immunodeficient mouse strains containing various human genes, including cytokines or HLA, has enabled the reconstitution of functional human immune systems after transplantation of human hematopoietic stem cells (HSC). Accumulating evidence has suggested that HLA-restricted antigen-specific human T-cell responses can be generated in these humanized mice. To directly monitor immune responses of human CD4{sup +} T cells, we introduced β-lactoglobulin (BLG)-specific T cell receptor (TCR) genes derived from CD4{sup +} T-cell clones of cow-milk allergy patients into HSCs, and subsequently transplanted them into NOG-HLA-DR4 transgenic/I-Aβ deficient mice (NOG-DR4/I-A{sup o}). In the thymus, thymocytes with BLG-specific TCR preferentially differentiated into CD4{sup +}CD8{sup −} single-positive cells. Adoptive transfer of mature CD4{sup +} T cells expressing the TCR into recipient NOG-DR4/I-A{sup o} mice demonstrated that human CD4{sup +} T cells proliferated in response to antigenic stimulation and produced IFN-γ in vivo, suggesting that functional T-cell reactions (especially Th1-skewed responses) were induced in humanized mice.

  17. Caffeine increases striatal dopamine D2/D3 receptor availability in the human brain

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Volkow, N. D.; Wang, G. -J.; Logan, J.; Alexoff, D.; Fowler, J. S.; Thanos, P. K.; Wong, C.; Casado, V.; Ferre, S.; Tomasi, D.

    2015-04-14

    Caffeine, the most widely consumed psychoactive substance in the world, is used to promote wakefulness and enhance alertness. Like other wake-promoting drugs (stimulants and modafinil), caffeine enhances dopamine (DA) signaling in the brain, which it does predominantly by antagonizing adenosine A2A receptors (A2AR). However, it is unclear if caffeine, at the doses consumed by humans, increases DA release or whether it modulates the functions of postsynaptic DA receptors through its interaction with adenosine receptors, which modulate them. We used positron emission tomography and [11C]raclopride (DA D2/D3 receptor radioligand sensitive to endogenous DA) to assess if caffeine increased DA release inmore » striatum in 20 healthy controls. Caffeine (300mg p.o.) significantly increased the availability of D2/D3 receptors in putamen and ventral striatum, but not in caudate, when compared with placebo. In addition, caffeine-induced increases in D2/D3 receptor availability in the ventral striatum were associated with caffeine-induced increases in alertness. Our findings indicate that in the human brain, caffeine, at doses typically consumed, increases the availability of DA D2/D3 receptors, which indicates that caffeine does not increase DA in the striatum for this would have decreased D2/D3 receptor availability. Instead, we interpret our findings to reflect an increase in D2/D3 receptor levels in striatum with caffeine (or changes in affinity). Furthermore, the association between increases in D2/D3 receptor availability in ventral striatum and alertness suggests that caffeine might enhance arousal, in part, by upregulating D2/D3 receptors.« less

  18. supplemental_lists_1d-2d-3c_06-24-2011.xls | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    lists1d-2d-3c06-24-2011.xls supplementallists1d-2d-3c06-24-2011.xls Office spreadsheet icon supplementallists1d-2d-3c06-24-2011.xls More Documents & Publications...

  19. He I D3 OBSERVATIONS OF THE 1984 MAY 22 M6.3 SOLAR FLARE

    SciTech Connect (OSTI)

    Liu Chang; Xu Yan; Deng Na; Lee, Jeongwoo; Zhang Jifeng; Wang Haimin; Prasad Choudhary, Debi

    2013-09-01

    The He I D3 line has a unique response to a flare impact on the low solar atmosphere and can be a powerful diagnostic tool for energy transport processes. Using images obtained from the recently digitized films of the Big Bear Solar Observatory, we report D3 observations of the M6.3 flare on 1984 May 22, which occurred in an active region with a circular magnetic polarity inversion line (PIL). The impulsive phase of the flare starts with a main elongated source that darkens in D3, inside of which bright emission kernels appear at the time of the initial small peak in hard X-rays (HXRs). These flare cores subsequently evolve into a sharp emission strand lying within the dark halo; this evolution occurs at the same time as the main peak in HXRs, reversing the overall source contrast from -5% to 5%. The radiated energy in D3 during the main peak is estimated to be about 10{sup 30} erg, which is comparable to that carried by nonthermal electrons above 20 keV. Afterward, the flare proceeds along the circular PIL in the counterclockwise direction to form a dark circular ribbon in D3, which apparently mirrors the bright ribbons in H{alpha} and He I 10830 A. All of these ribbons last for over one hour in the late gradual phase. We suggest that the present event resembles the so-called black-light flare that was proposed based on continuum images, and that D3 darkening and brightening features herein may be due to thermal conduction heating and the direct precipitation of high-energy electrons, respectively.

  20. 2D?3D polycatenated and 3D?3D interpenetrated metalorganic frameworks constructed from thiophene-2,5-dicarboxylate and rigid bis(imidazole) ligands

    SciTech Connect (OSTI)

    Erer, Hakan; Ye?ilel, Okan Zafer; Ar?c?, Mrsel; Keskin, Seda; Bykgngr, Orhan

    2014-02-15

    Hydrothermal reactions of rigid 1,4-bis(imidazol-1-yl)benzene (dib) and 1,4-bis(imidazol-1-yl)-2,5-dimethylbenzene (dimb) with deprotonated thiophene-2,5-dicarboxylic acid (H{sub 2}tdc) in the presence of Zn(II) and Cd(II) salts in H{sub 2}O produced three new metalorganic frameworks, namely, [Zn(-tdc)(H{sub 2}O)(-dib)]{sub n} (1), [Cd(-tdc)(H{sub 2}O)(-dib)]{sub n} (2), and ([Cd{sub 2}({sub 3}-tdc){sub 2}(-dimb){sub 2}](H{sub 2}O)){sub n}(3). These MOFs were characterized by FT-IR spectroscopy, elemental, thermal (TG, DTA, DTG and DSC), and single-crystal X-ray diffraction analyses. Isomorphous complexes 1 and 2 reveal polycatenated 2D+2D?3D framework based on an undulated (4,4)-sql layer. Complex 3 exhibits a new 4-fold interpenetrating 3D framework with the point symbol of 6{sup 6}. Molecular simulations were used to assess the potentials of the complexes for H{sub 2} storage application. Moreover, these coordination polymers exhibit blue fluorescent emission bands in the solid state at room temperature. - Graphical abstract: In this study, hydrothermal reactions of rigid 1,4-bis(imidazol-1-yl)benzene (dib) and 1,4-bis(imidazol-1-yl)-2,5-dimethylbenzene (dimb) with deprotonated thiophene-2,5-dicarboxylic acid (H{sub 2}tdc) in the presence of Zn(II) and Cd(II) salts in H{sub 2}O produced three new metalorganic frameworks. Isomorphous complexes 1 and 2 reveal polycatenated 2D+2D?3D framework based on an undulated (4,4)-sql layer. Complex 3 exhibits a new 4-fold interpenetrating 3D framework with the point symbol of 6{sup 6}. Molecular simulations were used to assess the potentials of the complexes for H{sub 2} storage application. These coordination polymers exhibit blue fluorescent emission bands in the solid state at room temperature. Display Omitted - Highlights: Complexes 1 and 2 display polycatenated 2D+2D?3D framework. Complex 3 exhibits a new 4-fold interpenetrating 3D framework. Complex 1 adsorbs the highest amount of H{sub 2} at 100 bar and

  1. Evaluation of static pressure drops and PM10 and TSP emissions for modified 1D-3D cyclones

    SciTech Connect (OSTI)

    Holt, G.A.; Baker, R.V.; Hughs, S.E.

    1999-12-01

    Five modifications of a standard 1D3D cyclone were tested and compared against the standard 1D3D design in the areas of particulate emissions and static pressure drop across the cyclone. The modifications to the 1D3D design included a 2D2D inlet, a 2D2D air outlet, a D/3 trash exit, an expansion chamber with a D/3 trash exit, and a tapered air outlet duct. The 1D3D modifications that exhibited a significant improvement in reducing both PM10 and total suspended particulate (TSP) emissions were the designs with the 2D2D inlet and air exhaust combined with either the conical D/3 tail cone or the expansion chamber. In reference to the standard 1D3D cyclone, the average reduction in PM10 emissions was 24 to 29% with a 29 to 35% reduction observed in TSP emissions. The modifications with the tapered air outlets did not show any significant improvements in controlling PM10 emissions. However, the modification with the tapered air outlet/expansion chamber combination exhibited statistical significance in reducing TSP emissions by 18% compared to the 1D3D cyclone. All modifications tested exhibited lower static pressure drops than the standard 1D3D.

  2. Data:12a45141-7972-4ec8-a2dc-37fedc8133d3 | Open Energy Information

    Open Energy Info (EERE)

    ec8-a2dc-37fedc8133d3 No revision has been approved for this page. It is currently under review by our subject matter experts. Jump to: navigation, search Loading... 1. Basic...

  3. Project Transition/Closeout (CD-4)

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2008-09-24

    This Guide is written for the federal project director ensuring that processes progress smoothly and that projects meet asset management goals and financial closure requirements of DOE.

  4. 3d-3p transitions in (. mu. /sup -/He/sup 4/)/sup +/

    SciTech Connect (OSTI)

    May, M.

    1986-01-01

    An experiment to measure the energy of 3d-3p transitions in the (..mu../sup -/He/sup 4/)/sup +/ ion is now in progress. The experiment, which is being performed at the Brookhaven National Laboratory Alternating Gradient Synchrotron, will use an infrared CO/sub 2/ laser to stimulate the transitions. These transitions are of interest because their energy is due almost entirely to the polarization of the vacuum. In a pure Coulomb field, states with the same principal quantum number, n, and total angular momentum, J, are degenerate. Vacuum polarization, because of its nonlinear dependence on electric field strength, results in departure from an inverse square Coulomb field, causing a splitting which depends on the orbital angular momentum, removing the degeneracy. The dominance of vacuum polarization in giving rise to these splittings in the muonic ion is in contrast to the situation in electronic atoms where vacuum polarization makes a very minor contribution to the Lamb shift. 4 refs., 4 figs.

  5. Uptake of donor lymphocytes treated with 8-methoxypsoralen and ultraviolet A light by recipient dendritic cells induces CD4{sup +}CD25{sup +}Foxp3{sup +} regulatory T cells and down-regulates cardiac allograft rejection

    SciTech Connect (OSTI)

    Zheng, De-Hua; Dou, Li-Ping; Wei, Yu-Xiang; Du, Guo-Sheng; Zou, Yi-Ping; Song, Ji-Yong; Zhu, Zhi-Dong; Cai, Ming; Qian, Ye-Yong; Shi, Bing-Yi

    2010-05-14

    Extracorporeal photopheresis (ECP) is an effective immunomodulatory therapy and has been demonstrated to be beneficial for graft-vs-host disease and solid-organ allograft rejection. ECP involves reinfusion of a patient's autologous peripheral blood leukocytes treated ex vivo with 8-methoxypsoralen and UVA light radiation (PUVA). Previous studies focused only on ECP treatment of recipient immune cells. Our study is the first to extend the target of ECP treatment to donor immune cells. The results of in vitro co-culture experiments demonstrate uptake of donor PUVA-treated splenic lymphocytes (PUVA-SPs) by recipient immature dendritic cells (DCs). Phagocytosis of donor PUVA-SPs does not stimulate phenotype maturation of recipient DCs. In the same co-culture system, donor PUVA-SPs enhanced production of interleukin-10 and interferon-{gamma} by recipient DCs and impaired the subsequent capability of recipient DCs to stimulate recipient naive T cells. Phagocytosis of donor PUVA-SP (PUVA-SP DCs) by recipient DCs shifted T-cell responses in favor of T helper 2 cells. Infusion of PUVA-SP DCs inhibited cardiac allograft rejection in an antigen-specific manner and induced CD4{sup +}CD25{sup high}Foxp3{sup +} regulatory T cells. In conclusion, PUVA-SP DCs simultaneously deliver the donor antigen and the regulatory signal to the transplant recipient, and thus can be used to develop a novel DC vaccine for negative immune regulation and immune tolerance induction.

  6. Outcome of the First wwPDB/CCDC/D3R Ligand Validation Workshop

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Adams, Paul  D.; Aertgeerts, Kathleen; Bauer, Cary; Bell, Jeffrey A.; Berman, Helen  M.; Bhat, Talapady  N.; Blaney, Jeff  M.; Bolton, Evan; Bricogne, Gerard; Brown, David; et al

    2016-04-05

    Crystallographic studies of ligands bound to biological macromolecules (proteins and nucleic acids) represent an important source of information concerning drug-target interactions, providing atomic level insights into the physical chemistry of complex formation between macromolecules and ligands. Of the more than 115,000 entries extant in the Protein Data Bank archive, ~75% include at least one non-polymeric ligand. Ligand geometrical and stereochemical quality, the suitability of ligand models for in silico drug discovery/design, and the goodness-of-fit of ligand models to electron density maps vary widely across the archive. We describe the proceedings and conclusions from the first Worldwide Protein Data Bank/Cambridge Crystallographicmore » Data Centre/Drug Design Data Resource (wwPDB/CCDC/D3R) Ligand Validation Workshop held at the Research Collaboratory for Structural Bioinformatics at Rutgers University on July 30-31, 2015. Experts in protein crystallography from academe and industry came together with non-profit and for-profit software providers for crystallography and with experts in computational chemistry and data archiving to discuss and make recommendations on best practices, as framed by a series of questions central to structural studies of macromolecule-ligand complexes. What data concerning bound ligands should be archived in the Protein Data Bank? How should the ligands be best represented? How should structural models of macromolecule-ligand complexes be validated? What supplementary information should accompany publications of structural studies of biological macromolecules? Consensus recommendations on best practices developed in response to each of these questions are provided, together with some details regarding implementation. Important issues addressed but not resolved at the workshop are also enumerated.« less

  7. Study of plasma meniscus formation and beam halo in negative ion source using the 3D3VPIC model

    SciTech Connect (OSTI)

    Nishioka, S.; Goto, I.; Hatayama, A.; Miyamoto, K.; Fukano, A.

    2015-04-08

    In this paper, the effect of the electron confinement time on the plasma meniscus and the fraction of the beam halo is investigated by 3D3V-PIC (three dimension in real space and three dimension in velocity space) (Particle in Cell) simulation in the extraction region of negative ion source. The electron confinement time depends on the characteristic time of electron escape along the magnetic field as well as the characteristic time of diffusion across the magnetic field. Our 3D3V-PIC results support the previous result by 2D3V-PIC results i.e., it is confirmed that the penetration of the plasma meniscus becomes deep into the source plasma region when the effective confinement time is short.

  8. Remaining Sites Verification Package for 132-D-3, 1608-D Effluent Pumping Station, Waste Site Reclassification Form 2005-033

    SciTech Connect (OSTI)

    R. A. Carlson

    2006-05-09

    Decommissioning and demolition of the 132-D-3 site, 1608-D Effluent Pumping Station was performed in 1986. Decommissioning included removal of equipment, water, and sludge for disposal as radioactive waste. The at- and below-grade structure was demolished to at least 1 m below grade and the resulting rubble buried in situ. The area was backfilled to grade with at least 1 m of clean fill and contoured to the surrounding terrain. Residual concentrations support future land uses that can be represented by a rural-residential scenario and pose no threat to groundwater or the Columbia River based on RESRAD modeling.

  9. Synthesis and structural characterization of the new clathrates K8Cd4Ge42, Rb8Cd4Ge42, and Cs8Cd4Ge42

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Schafer, Marion; Bobev, Svilen

    2016-03-25

    This paper presents results from our exploratory work in the systems K-Cd-Ge, Rb-Cd-Ge, and Cs-Cd-Ge, which yielded the novel type-I clathrates with refined compositions K8Cd3.77(7)Ge42.23, Rb8Cd3.65(7)Ge42.35, and Cs7.80(1)Cd3.65(6)Ge42.35. The three compounds represent rare examples of clathrates of germanium with the alkali metals, where a d10 element substitutes a group 14 element. The three structures, established by single-crystal X-ray diffraction, indicate that the framework-building Ge atoms are randomly substituted by Cd atoms on only one of the three possible crystallographic sites. Furthermore, this and several other details of the crystal chemistry are elaborated.

  10. u.s. DEPARIUENI OF ENERGY EERE PROJECT MAN AG EMENT CENTER NEPA...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    available data to predict the most likely locations for high fracture density and hot fluids. The identified locations would be used to site and drill two full-size production...

  11. u.s. DEPARThIENT OF ENERGY EERE PROJECT MANAG EMENT CENTER NFPA...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Inc. (BGI) would utilize DOE and cost share funds to (1) combine airborne full tensor gravity (FTG) and the z-axis tipper electromagnetic (ZTEM) deep penetrating, low frequency,...

  12. Exotic magnetism on the quasi-FCC lattices of the d3 double perovskites La2NaB'O6 (B' = Ru, Os)

    SciTech Connect (OSTI)

    Aczel, Adam A; Baker, Peter J.; Bugaris, Dan; Yeon, Jeongho; Zur Loye, Hans-Conrad; Guidi, T.; Adroja, D. T.

    2014-01-01

    We find evidence for long-range and short-range ($\\zeta$~$=$~70~\\AA~at 4~K) incommensurate magnetic order on the quasi-face-centered-cubic (FCC) lattices of the monoclinic double perovskites La$_2$NaRuO$_6$ and La$_2$NaOsO$_6$ respectively. Incommensurate magnetic order on the FCC lattice has not been predicted by mean field theory, but may arise via a delicate balance of inequivalent nearest neighbour and next nearest neighbour exchange interactions. In the Ru system with long-range order, inelastic neutron scattering also reveals a spin gap $\\Delta$~$\\sim$~2.75~meV. Magnetic anisotropy is generally minimized in the more familiar octahedrally-coordinated $3d^3$ systems, so the large gap observed for La$_2$NaRuO$_6$ may result from the significantly enhanced value of spin-orbit coupling in this $4d^3$ material.

  13. DOE CD-4 Review of the MINERvA Project

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Assessment Document (with signatures) (pdf) Integrated Safety Management Document (pdf) Burn Test Results (pdf) Project Execution Plan (22207 Version with signatures) (pdf) Other...

  14. SU-E-J-13: Six Degree of Freedom Image Fusion Accuracy for Cranial Target Localization On the Varian Edge Stereotactic Radiosurgery System: Comparison Between 2D/3D and KV CBCT Image Registration

    SciTech Connect (OSTI)

    Xu, H; Song, K; Chetty, I; Kim, J; Wen, N

    2015-06-15

    Purpose: To determine the 6 degree of freedom systematic deviations between 2D/3D and CBCT image registration with various imaging setups and fusion algorithms on the Varian Edge Linac. Methods: An anthropomorphic head phantom with radio opaque targets embedded was scanned with CT slice thicknesses of 0.8, 1, 2, and 3mm. The 6 DOF systematic errors were assessed by comparing 2D/3D (kV/MV with CT) with 3D/3D (CBCT with CT) image registrations with different offset positions, similarity measures, image filters, and CBCT slice thicknesses (1 and 2 mm). The 2D/3D registration accuracy of 51 fractions for 26 cranial SRS patients was also evaluated by analyzing 2D/3D pre-treatment verification taken after 3D/3D image registrations. Results: The systematic deviations of 2D/3D image registration using kV- kV, MV-kV and MV-MV image pairs were within ±0.3mm and ±0.3° for translations and rotations with 95% confidence interval (CI) for a reference CT with 0.8 mm slice thickness. No significant difference (P>0.05) on target localization was observed between 0.8mm, 1mm, and 2mm CT slice thicknesses with CBCT slice thicknesses of 1mm and 2mm. With 3mm CT slice thickness, both 2D/3D and 3D/3D registrations performed less accurately in longitudinal direction than thinner CT slice thickness (0.60±0.12mm and 0.63±0.07mm off, respectively). Using content filter and using similarity measure of pattern intensity instead of mutual information, improved the 2D/3D registration accuracy significantly (P=0.02 and P=0.01, respectively). For the patient study, means and standard deviations of residual errors were 0.09±0.32mm, −0.22±0.51mm and −0.07±0.32mm in VRT, LNG and LAT directions, respectively, and 0.12°±0.46°, −0.12°±0.39° and 0.06°±0.28° in RTN, PITCH, and ROLL directions, respectively. 95% CI of translational and rotational deviations were comparable to those in phantom study. Conclusion: 2D/3D image registration provided on the Varian Edge radiosurgery, 6 DOF

  15. Final Hazard Categorization for the Remediation of the 118-D-1, 118-D-2, 118-D-3, 118-H-1, 118-H-2 and 118-H-3 Solid Waste Burial Grounds

    SciTech Connect (OSTI)

    K. L. Vialetti

    2008-05-20

    This report presents the final hazard categorization for the remediation of the 118-D-1, 118-D-2, and 118-D-3 Burial Grounds located within the 100-D/DR Area of the Hanford Site and the 118-H-1, 118-H-2, and 118-H-3 Burial Grounds located within the 100-H Area of the Hanford Site.

  16. Final Hazard Categorization for the Remediation of the 118-D-1, 118-D-2, 118-D-3, 118-H-1, 118-H-2, and 118-H-3 Solid Waste Burial Grounds

    SciTech Connect (OSTI)

    T. J. Rodovsky

    2006-12-06

    This report presents the final hazard categorization (FHC) for the remediation of the 118-D-1, 118-D-2, and 118-D-3 Burial Grounds located within the 100-D/DR Area of the Hanford Site and the 118-H-1, 118-H-2, and 118-H-3 Burial Grounds located within the 100-H Area of the Hanford Site.

  17. Final Hazard Categorization for the Remediation of the 118-D-1, 118-D-2, 118-D-3, 118-H-1, 118-H-2, and 118-H-3 Solid Waste Burial Grounds

    SciTech Connect (OSTI)

    T. J. Rodovsky

    2007-04-12

    This report presents the final hazard categorization (FHC) for the remediation of the 118-D-1, 118-D-2, and 118-D-3 Burial Grounds located within the 100-D/DR Area of the Hanford Site and the 118-H-1, 118-H-2, and 118-H-3 Burial Grounds located within the 100-H Area of the Hanford Site.

  18. Final Hazard Categorization for the Remediation of the 118-D-1, 118-D-2, 118-D-3, 118-H-1, 118-H-2, and 118-H-3 Solid Waste Burial Grounds

    SciTech Connect (OSTI)

    J.D. Ludowise

    2009-06-17

    This report presents the final hazard categorization for the remediation of the 118-D-1, 118-D-2, 118-D-3 Burial Grounds located within the 100-D/DR Area of the Hanford Site and the 118-H-1, 118-H-2, and 118-H-3 Burial Grounds located within the 100-H Area of the Hanford Site. A material at risk calculation was performed that determined the radiological inventory for each burial ground to be Hazard Category 3.

  19. Final Hazard Categorization and Auditable Safety Analysis for the Remediation of the 118-D-1, 118-D-2, 118-D-3, 118-H-1, 118-H-2 and 118-H-3 Solid Waste Burial Grounds

    SciTech Connect (OSTI)

    T. J. Rodovsky

    2006-03-01

    This report presents the initial hazard categorization, final hazard categorization and auditable safety analysis for the remediation of the 118-D-1, 118-D-2, and 118-D-3 Burial Grounds located within the 100-D/DR Area of the Hanford Site and the 118-H-1, 118-H-2, and 118-H-3 Burial Grounds located within the 100-H Area of the Hanford Site.

  20. D3 website database | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    More Documents & Publications Advanced Vehicle Benchmarking of HEVs and PHEVs Argonne Facilitation of PHEV Standard Testing Procedure (SAE J1711) AVTA: 2012 Toyota Prius PHEV ...

  1. Small-Molecule CD4-Mimics: Structure-Based Optimization of HIV...

    Office of Scientific and Technical Information (OSTI)

    S. ; Freire, Ernesto ; Sodroski, Joseph ; Madani, Navid ; Hendrickson, Wayne A. ; Smith, III, Amos B. 1 ; DFCI) 2 ; UPENN) 2 ; JHU) 2 ; Bryn Mawr) 2 ; Harvard-Med) ...

  2. Materials Data on Ca9Cd4Bi9 (SG:55) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2014-11-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  3. Materials Data on Cd4SiSe6 (SG:9) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2014-11-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  4. Materials Data on Cd4SiS6 (SG:9) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2014-11-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  5. Materials Data on Cd4GeSe6 (SG:9) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2014-11-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  6. Materials Data on Sr9Cd4Bi9 (SG:55) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2015-03-09

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  7. Stereochemical determination of carbon partitioning between photosynthesis and photorespiration in C3 plants: use of (3R)-D-(3-3H1, 3-14C)glyceric acid

    SciTech Connect (OSTI)

    Hanson, K.R.

    1984-07-01

    When (3R)-D-(3-3H1,3-14C)glyceric acid is supplied in tracer amounts to illuminated tobacco leaf discs, the acid penetrates to the chloroplasts without loss of 3H, and is phosphorylated there. Subsequent metabolism associated with the reductive photosynthetic cycle fully conserves 3H. Oxidation of ribulose bisphosphate (RuBP) by RuBP carboxylase-oxygenase (EC 4.1.1.39) results in the formation of (2R)-(2-3H1, 14C)glycolic acid which, on oxidation by glycolate oxidase (EC 1.1.3.1), releases 3H to water. Loss of 3H from the combined photosynthetic and photorespiratory systems is, therefore, associated with the oxidative photorespiratory loop. Assuming steady-state conditions and a basic metabolic model, the fraction of RuBP oxidized and the photorespiratory carbon flux relative to gross or net CO2 fixation can be calculated from the fraction of supplied 3H retained in the triose phosphates exported from the chloroplasts. This retention can be determined from the 3H:14C ratio for glucose obtained from isolated sucrose. The dependence of 3H retention upon O2 and CO2 concentrations can be deduced by assuming simple competitive kinetics for RuBP carboxylase-oxygenase. The experimental results confirmed the stereochemical assumptions made. Under conditions of negligible photorespiration 3H retention was essentially complete. The change in 3H retention with O2 and CO2 concentrations were investigated. For leaf discs (upper surface up) in normal air, it was estimated that 39% of the RuBP was oxidized, 32% of the fixed CO2 was photorespired, and the photorespiration rate was 46% of the net photosynthetic CO2 fixation rate. These are minimal estimates, as it is assumed that the only source of photorespired CO2 is glycine decarboxylation.

  8. CEBAFatAGlance_11x17_d3

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

  9. Alarm Management System for the D/3 Distributed Control System

    Energy Science and Technology Software Center (OSTI)

    1997-03-19

    As industrial processes continue to grow in size and complexity, the Distrubuted Control Systems that automate and monitor these processes expand in a like manner. This increase in control system complexity has resulted in ever increasing numbers of alarms presented to the operator. The challenge for today's control system designer is to find innovative ways to present alarm information to the operator such that despite the large number of alarms, the operator is able tomore » quickly assess the status of the plant and immediately respond to the most critical alarms in a timely manner. This software package, designed and developed for the Savannah River Site Replacement High Level Waste Evaporator/Waste Removal Distributed Control System installed on the H-Area Tank Farm, provides an alarm system which utilizes the annunciator (SKID) panel as a means of statusing the plant and providing single keystroke access to the display on which an alarm resides.« less

  10. Microsoft Word - Policy Flash 2010-81 Attachment 2

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ------ ----- agement r 70-31 C, " ----- ------ Require Contractor ------ ---Chapt ements Legal Man ------ ter 31.3 (Sep nagement Re ------ ptember 2010 equirement -- 0) ts."

  11. Study of negative hydrogen ion beam optics using the 3D3V PIC model

    SciTech Connect (OSTI)

    Miyamoto, K.; Nishioka, S.; Goto, I.; Hatayama, A.; Hanada, M.; Kojima, A.

    2015-04-08

    The mechanism of negative ion extraction under real conditions with the complex magnetic field is studied by using the 3D PIC simulation code. The extraction region of the negative ion source for the negative ion based neutral beam injection system in fusion reactors is modelled. It is shown that the E x B drift of electrons is caused by the magnetic filter and the electron suppression magnetic field, and the resultant asymmetry of the plasma meniscus. Furthermore, it is indicated that that the asymmetry of the plasma meniscus results in the asymmetry of negative ion beam profile including the beam halo. It could be demonstrated theoretically that the E x B drift is not significantly weakened by the elastic collisions of the electrons with neutral particles.

  12. A 3D-3C Reflection Seismic Survey and Data Integration to Identify...

    Open Energy Info (EERE)

    geophysical and wellbore data for the area, and these data will be complemented with modern, state-of-the-art reflection seismic data. Three-component geophones will record...

  13. Elliptic and Triangular Flow in Event-by-Event D=3+1 Viscous...

    Office of Scientific and Technical Information (OSTI)

    Department of Physics, McGill University, 3600 University Street, Montreal, Quebec, H3A 2T8 (Canada) Publication Date: 2011-01-28 OSTI Identifier: 21532263 Resource Type: Journal ...

  14. Enhanced pairing of quantum critical metals near d = 3 + 1 (Journal...

    Office of Scientific and Technical Information (OSTI)

    GrantContract Number: AC02-76SF00515 Type: Publisher's Accepted Manuscript Journal Name: Physical Review B Additional Journal Information: Journal Volume: 92; Journal Issue: 4; ...

  15. 3D/3D registration of coronary CTA and biplane XA reconstructions for improved image guidance

    SciTech Connect (OSTI)

    Dibildox, Gerardo Baka, Nora; Walsum, Theo van; Punt, Mark; Aben, Jean-Paul; Schultz, Carl; Niessen, Wiro

    2014-09-15

    Purpose: The authors aim to improve image guidance during percutaneous coronary interventions of chronic total occlusions (CTO) by providing information obtained from computed tomography angiography (CTA) to the cardiac interventionist. To this end, the authors investigate a method to register a 3D CTA model to biplane reconstructions. Methods: The authors developed a method for registering preoperative coronary CTA with intraoperative biplane x-ray angiography (XA) images via 3D models of the coronary arteries. The models are extracted from the CTA and biplane XA images, and are temporally aligned based on CTA reconstruction phase and XA ECG signals. Rigid spatial alignment is achieved with a robust probabilistic point set registration approach using Gaussian mixture models (GMMs). This approach is extended by including orientation in the Gaussian mixtures and by weighting bifurcation points. The method is evaluated on retrospectively acquired coronary CTA datasets of 23 CTO patients for which biplane XA images are available. Results: The Gaussian mixture model approach achieved a median registration accuracy of 1.7 mm. The extended GMM approach including orientation was not significantly different (P > 0.1) but did improve robustness with regards to the initialization of the 3D models. Conclusions: The authors demonstrated that the GMM approach can effectively be applied to register CTA to biplane XA images for the purpose of improving image guidance in percutaneous coronary interventions.

  16. A 3D-3C Reflection Seismic Survey and Data Integration to Identify...

    Broader source: Energy.gov (indexed) [DOE]

    Project Summary: Understanding geothermal reservoirs requires multi-discipline, integrated 3D GIS: Access down hole geophysical logs, surface geophysics, isotherms, isoresistivity ...

  17. E~ S*D3 Weldon Spring Site Environmental Repon lor Calendar...

    Office of Legacy Management (LM)

    ... DATA SOURCES VARY. QUATERNARY UNIT TJCKNESS BASED ON ON-SITE DRILLING AND lR(NCH lNG. BURLINGTON AND KEOKUK THROUGH JOACHIM DOLOMITE BASED ON USGS *ELLS IIW-GS02 AND CSOS. ...

  18. C:\DOCUME~1\d3k776\LOCALS~1\TEM

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Talking About Climate at Schools in the Tropical Western Pacific (continued on page 3) Education Climate Update ARM News from the ARM Program for students and teachers about climate change studies IN THIS ISSUE TWP Classroom Visits Elder Knowledge Student Activity Critical Thinking ? WCS Conservation Teacher's Experience Dr. Bill Porch, ARM Science Programs Specialist W WINTER 2002 hen I visit the ARM sites at Manus Island, Papua New Guinea and the Island Nation of Nauru, I am often asked to

  19. ch_6

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    0 6.0 Sta Sta tutes tutes , , Regula Regula tions tions , , Consulta Consulta tions tions , , and Other and Other Requir Requir ements ements 6-1 DOE/EIS-0287 This chapter discusses the consultations and coordination the U.S. Department of Energy (DOE) has had with various agen- cies during the preparation of this Environmental Impact Statement (EIS). This chapter also analyzes the complex regulatory issues that arise when consider- ing the various alternatives discussed pre- viously. When

  20. Spontaneous breaking of scale invariance in a D = 3 U(N ) model with Chern-Simons gauge fields

    SciTech Connect (OSTI)

    Bardeen, William A.; Moshe, Moshe

    2014-06-18

    We study spontaneous breaking of scale invariance in the large N limit of three dimensional U(N )κ Chern-Simons theories coupled to a scalar field in the fundamental representation. When a λ6 ( Ø· Ø)3 self interaction term is added to the action we find a massive phase at a certain critical value for a combination of the λ(6) and ’t Hooft’s λ = N/κ couplings. This model attracted recent attention since at finite κ it contains a singlet sector which is conjectured to be dual to Vasiliev’s higher spin gravity on AdS4. Our paper concentrates on the massive phase of the 3d boundary theory. We discuss the advantage of introducing masses in the boundary theory through spontaneous breaking of scale invariance.

  1. Negative Ion Photoelectron Spectroscopy Confirms the Prediction that D-3h Carbontrioxide (CO3) Has a Singlet Ground State

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Hrovat, David; Hou, Gao-Lei; Chen, Bo; Wang, Xue B.; Borden, Weston

    2015-11-13

    The CO3 radical anion (CO3•–) has been formed by electrospraying carbonate dianion (CO32–) into the gas phase. The negative ion photoelectron (NIPE) spectrum of CO3•– shows that, unlike trimethylenemethane [C(CH2)3], carbontrioxide (CO3) has a singlet ground state. From the NIPE spectrum, the electron affinity of CO3 was determined to be EA = 4.06 ± 0.03 eV, and the singlet-triplet energy difference was found to be ΔEST = - 17.8 ± 0.9 kcal/mol. B3LYP, CCSD(T), and CASPT2 calculations all find that the two lowest triplet states of CO3 are very close in energy, a prediction that is confirmed by the relativemore » intensities of the bands in the NIPE spectrum of CO3•–. The 560 cm-1 vibrational progression, seen in the low energy region of the triplet band, enables the identification of the lowest, Jahn-Teller-distorted, triplet state as 3A1, in which both unpaired electrons reside in σ MOs, rather than 3A2, in which one unpaired electron occupies the b2 σ MO, and the other occupies the b1 π MO.« less

  2. The massive fermion phase for the U(N) Chern-Simons gauge theory in D=3 at large N

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Bardeen, William A.

    2014-10-07

    We explore the phase structure of fermions in the U(N) Chern-Simons Gauge theory in three dimensions using the large N limit where N is the number of colors and the fermions are taken to be in the fundamental representation of the U(N) gauge group. In the large N limit, the theory retains its classical conformal behavior and considerable attention has been paid to possible AdS/CFT dualities of the theory in the conformal phase. In this paper we present a solution for the massive phase of the fermion theory that is exact to the leading order of ‘t Hooft’s large Nmore » expansion. We present evidence for the spontaneous breaking of the exact scale symmetry and analyze the properties of the dilaton that appears as the Goldstone boson of scale symmetry breaking.« less

  3. Instabilities of an anisotropically expanding non-Abelian plasma: 1D+3V discretized hard-loop simulations

    SciTech Connect (OSTI)

    Rebhan, Anton; Attems, Maximilian; Strickland, Michael

    2008-08-15

    Non-Abelian plasma instabilities play a crucial role in the nonequilibrium dynamics of a weakly coupled quark-gluon plasma, and they importantly modify the standard perturbative bottom-up thermalization scenario in heavy-ion collisions. Using the auxiliary-field formulation of the hard-loop effective theory, we study numerically the real-time evolution of instabilities in an anisotropic collisionless Yang-Mills plasma undergoing longitudinal free-streaming expansion. In this first real-time lattice simulation we consider the most unstable modes, long-wavelength coherent color fields that are constant in transverse directions and which therefore are effectively 1+1 dimensional in space-time, except for the auxiliary fields which also depend on discretized momentum rapidity and transverse velocity components. We reproduce the semianalytical results obtained previously for the Abelian regime, and we determine the nonlinear effects which occur when the instabilities have grown such that non-Abelian interactions become important.

  4. Spontaneous breaking of scale invariance in a D = 3 U(N ) model with Chern-Simons gauge fields

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Bardeen, William A.; Moshe, Moshe

    2014-06-18

    We study spontaneous breaking of scale invariance in the large N limit of three dimensional U(N )κ Chern-Simons theories coupled to a scalar field in the fundamental representation. When a λ6 ( ؆ · Ø)3 self interaction term is added to the action we find a massive phase at a certain critical value for a combination of the λ(6) and ’t Hooft’s λ = N/κ couplings. This model attracted recent attention since at finite κ it contains a singlet sector which is conjectured to be dual to Vasiliev’s higher spin gravity on AdS4. Our paper concentrates on the massive phasemore » of the 3d boundary theory. We discuss the advantage of introducing masses in the boundary theory through spontaneous breaking of scale invariance.« less

  5. Magnetic order and electronic structure of 5d3 double perovskite Sr2ScOsO6

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Taylor, A. E.; Morrow, R.; Singh, D. J.; Calder, S.; Lumsden, M. D.; Woodward, P. M.; Christianson, A. D.

    2015-03-01

    The magnetic susceptibility, crystal and magnetic structures, and electronic structure of double perovskite Sr2ScOsO6 are reported. Using both neutron and x-ray powder diffraction we find that the crystal structure is monoclinic P21/n from 3.5 to 300 K. Magnetization measurements indicate an antiferromagnetic transition at TN=92 K, one of the highest transition temperatures of any double perovskite hosting only one magnetic ion. Type I antiferromagnetic order is determined by neutron powder diffraction, with an Os moment of only 1.6(1) muB, close to half the spin-only value for a crystal field split 5d electron state with t2g^3 ground state. Density functional calculationsmore » show that this reduction is largely the result of strong Os-O hybridization, with spin-orbit coupling responsible for only a ~0.1 muB reduction in the moment.« less

  6. The massive fermion phase for the U(N) Chern-Simons gauge theory in D=3 at large N

    SciTech Connect (OSTI)

    Bardeen, William A.

    2014-10-07

    We explore the phase structure of fermions in the U(N) Chern-Simons Gauge theory in three dimensions using the large N limit where N is the number of colors and the fermions are taken to be in the fundamental representation of the U(N) gauge group. In the large N limit, the theory retains its classical conformal behavior and considerable attention has been paid to possible AdS/CFT dualities of the theory in the conformal phase. In this paper we present a solution for the massive phase of the fermion theory that is exact to the leading order of t Hoofts large N expansion. We present evidence for the spontaneous breaking of the exact scale symmetry and analyze the properties of the dilaton that appears as the Goldstone boson of scale symmetry breaking.

  7. Magnetic order and electronic structure of 5d3 double perovskite Sr2ScOsO6

    SciTech Connect (OSTI)

    Calder, Stuart A; Morrow, Ryan; Taylor, Alice E; Lumsden, Mark D; Woodward, Patrick; Christianson, Andrew D; Singh, David J

    2015-01-01

    The magnetic susceptibility, crystal and magnetic structures, and electronic structure of double perovskite Sr2ScOsO6 are reported. Using both neutron and x-ray powder diffraction we find that the crystal structure is monoclinic P21/n from 3.5 to 300 K. Magnetization measurements indicate an antiferromagnetic transition at TN=92 K, one of the highest transition temperatures of any double perovskite hosting only one magnetic ion. Type I antiferromagnetic order is determined by neutron powder diffraction, with an Os moment of only 1.6(1) muB, close to half the spin-only value for a crystal field split 5d electron state with t2g^3 ground state. Density functional calculations show that this reduction is largely the result of strong Os-O hybridization, with spin-orbit coupling responsible for only a ~0.1 muB reduction in the moment.

  8. National Environmental Policy Act RM

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    National Environmental Policy Act Review Module March 2010 CD- N -0 OFFICE O National E C CD-1 OF ENVIRO Standa Environm Rev Critical Deci CD-2 M ONMENTA ard Review mental P view Modul ision (CD) A C March 2010 AL MANAG Plan olicy Act le Applicability D-3 GEMENT t (NEPA) CD-4 ) Post Ope eration Standard Review Plan, 2 nd Edition, March 2010 i FOREWORD The Standard Review Plan (SRP) 1 provides a consistent, predictable corporate review framework to ensure that issues and risks that could

  9. Negative Ion Photoelectron Spectroscopy Confirms the Prediction that D-3h Carbontrioxide (CO3) Has a Singlet Ground State

    SciTech Connect (OSTI)

    Hrovat, David; Hou, Gao-Lei; Chen, Bo; Wang, Xue B.; Borden, Weston

    2015-11-13

    The CO3 radical anion (CO3•–) has been formed by electrospraying carbonate dianion (CO32–) into the gas phase. The negative ion photoelectron (NIPE) spectrum of CO3•– shows that, unlike trimethylenemethane [C(CH2)3], carbontrioxide (CO3) has a singlet ground state. From the NIPE spectrum, the electron affinity of CO3 was determined to be EA = 4.06 ± 0.03 eV, and the singlet-triplet energy difference was found to be ΔEST = - 17.8 ± 0.9 kcal/mol. B3LYP, CCSD(T), and CASPT2 calculations all find that the two lowest triplet states of CO3 are very close in energy, a prediction that is confirmed by the relative intensities of the bands in the NIPE spectrum of CO3•–. The 560 cm-1 vibrational progression, seen in the low energy region of the triplet band, enables the identification of the lowest, Jahn-Teller-distorted, triplet state as 3A1, in which both unpaired electrons reside in σ MOs, rather than 3A2, in which one unpaired electron occupies the b2 σ MO, and the other occupies the b1 π MO.

  10. Data:94d06976-03c6-46f7-a433-abc5f4968d3a | Open Energy Information

    Open Energy Info (EERE)

    under review by our subject matter experts. Jump to: navigation, search Loading... 1. Basic Information 2. Demand 3. Energy << Previous 1 2 3 Next >> Basic Information...

  11. Section IV.D.3 for DOE 2013 Annual Report: Novel Phosphazene-based Compounds to Enhance Safety and Stability of Cell Chemistries for High Voltage Applications (INL)

    SciTech Connect (OSTI)

    Kevin L. Gering; Mason K. Harrup; Eric J. Dufek; Sergiy V. Sazhin; Harry W. Rollins; David K. Jamison; Fred F. Stewart; John Burba

    2013-09-01

    Electrolytes play a central role in performance and aging in most electrochemical systems. As automotive and grid applications place a higher reliance on electrochemical stored energy, it becomes more urgent to have electrolyte components that enable optimal battery performance while promoting battery safety and longevity. Safety remains a foremost concern for widespread utilization of Li-ion technology in electric-drive vehicles, especially as the focus turns to higher voltage systems (5V). This work capitalizes on the long established INL expertise regarding phosphazene chemistry, aimed at battery-viable compounds for electrolytes and electrodes that are highly tolerant to abusive conditions. This report showcases our 2013 work for the DOE applied battery research (ABR) program, wherein testing results are summarized for INL electrolytes and alternative anode materials.

  12. A 3D-3C Reflection Seismic Survey and Data Integration to Identify the Seismic Response of Fractures and Permeable Zones Over a Known Geothermal Resource at Soda Lake, Churchhill Co., NV

    Broader source: Energy.gov [DOE]

    DOE Geothermal Peer Review 2010 - Presentation. Project Summary: Understanding geothermal reservoirs requires multi-discipline, integrated 3D GIS: Access down hole geophysical logs, surface geophysics, isotherms, isoresistivity surfaces, seismic data, cross-sections, etc. instantaneously; Wells tell where youve been; MT shows the direction to go; Seismic provides the map; & Be prepared for discoveries.

  13. Integrated Biorefinery Research Facility (IBRF I-II) (Post CD...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Integrated Biorefinery Research Facility (IBRF I-II) (Post CD-4), EERE, Aug 2011 Integrated Biorefinery Research Facility (IBRF I-II) (Post CD-4), EERE, Aug 2011 PDF icon 000521 & ...

  14. High-Efficiency Parking Lighting in Federal Facilities

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    High-Efficiency Parking Lighting in Federal Facilities FEdEraL EnErgy ManagEMEnt PrograM MC Realty Group Saving Energy and Money with the IRS MC Realty Group, LLC, won a 2014 LEEP Award for cutting energy use by 76% at the Internal Revenue Service (IRS) Facility Parking Garage in Kansas City, Missouri. MC Realty replaced 1,500 metal halide fxtures with an equal number of T8 fuorescent fxtures in the fve-story parking structure to cut energy use by 2 million kilowatt-hours (kWh) annually, which

  15. DATE: TO: FROM:

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    52 May 17,2010 Procurement Directors Office of Procurement and Assistance Policy, MA-61 Office of Procurement and Assistance Management SUBJECT: Acquisition Letters Remaining In Effect SUMMARY: Acquisition Letter (AL) 2010-07 has been issued. It lists ALs currently in effect and the discontinued ALs along with the reason why the AL is no longer in effect. This flash may be viewed at http://mana~ement.energ;y.~ov/policy p;uidance/policy flashes.htm. Questions concerning this policy flash

  16. Charter

    Broader source: Energy.gov (indexed) [DOE]

    Version Sep 2011 DOE EVMS GOLD CARD PERFORMANCE BASELINE COMPONENTS (Performance Baseline must clearly document scopeKPPs, TPC and CD-4 date) AUW Authorized Unpriced Work ...

  17. Example BCP Template | Department of Energy

    Office of Environmental Management (EM)

    Forms and Templates More Documents & Publications Critical Decision 2 (CD-2) Approval Template Critical Decision 4 (CD-4) Approval Template External Independent Review (EIR) Report ...

  18. Search for: All records | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    ... and superfluidity (4) engineering (4) environmental sciences (4) geologic ... Small-Molecule CD4-Mimics: Structure-Based Optimization of HIV-1 Entry Inhibition Melillo, ...

  19. Approval of the Critical Decision 4.

    Office of Legacy Management (LM)

    of the closure. 1. CD-4Briefing to the Energy System Acquisition Advisory Board 2. LM Site ... Idaho National Engineering and Environmental Laboratory (INEEL) for interim storage. ...

  20. Pathways for Implementing REDD+: Experiences from Carbon Markets...

    Open Energy Info (EERE)

    Centre Sector: Land Focus Area: Forestry Topics: Finance, Implementation Resource Type: Lessons learnedbest practices Website: www.acp-cd4cdm.orgmedia237951...

  1. Model-independent determination of the astrophysical S factor in laser-induced fusion plasmas

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Lattuada, D.; Barbarino, M.; Bonasera, A.; Bang, W.; Quevedo, H. J.; Warren, M.; Consoli, F.; De Angelis, R.; Andreoli, P.; Kimura, S.; et al

    2016-04-01

    In this paper, we present a new and general method for measuring the astrophysical S factor of nuclear reactions in laser-induced plasmas and we apply it to 2H(d,n)3He. The experiment was performed with the Texas Petawatt Laser, which delivered 150–270 fs pulses of energy ranging from 90 to 180 J to D2 or CD4 molecular clusters (where D denotes 2H). After removing the background noise, we used the measured time-of-flight data of energetic deuterium ions to obtain their energy distribution. We derive the S factor using the measured energy distribution of the ions, the measured volume of the fusion plasma,more » and the measured fusion yields. This method is model independent in the sense that no assumption on the state of the system is required, but it requires an accurate measurement of the ion energy distribution, especially at high energies, and of the relevant fusion yields. In the 2H(d,n)3He and 3He(d,p)4He cases discussed here, it is very important to apply the background subtraction for the energetic ions and to measure the fusion yields with high precision. While the available data on both ion distribution and fusion yields allow us to determine with good precision the S factor in the d+d case (lower Gamow energies), for the d+3He case the data are not precise enough to obtain the S factor using this method. Our results agree with other experiments within the experimental error, even though smaller values of the S factor were obtained. This might be due to the plasma environment differing from the beam target conditions in a conventional accelerator experiment.« less

  2. Readiness Review RM

    Broader source: Energy.gov [DOE]

    This module assists DOE Federal line management in their efforts to bring a project or facility into a condition where it is sufficiently prepared to start or resume operations prior to CD-4...

  3. Safeguards and Security and Cyber Security RM

    Broader source: Energy.gov [DOE]

    The SSCS RM is a tool that assists the DOE federal project review teams in evaluating the technical sufficiency of the project SSCS activities at CD-0 through CD-4.

  4. Seismic Design Expectations Report

    Office of Environmental Management (EM)

    March 2010 CD- This Rev of th Se -0 view Module w he overall Cons OFFICE O eismic De C ... CD-4 ldg. 3019 60% rned from this r ule. ) Post Ope design review review have be eration w ...

  5. Search for: All records | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    ... Have feedback or suggestions for a way to improve these results? Small-Molecule CD4-Mimics: Structure-Based Optimization of HIV-1 Entry Inhibition Melillo, Bruno ; Liang, Shuaiyi ...

  6. Microsoft PowerPoint - Johnson - How EM Does Business (FINAL...

    Broader source: Energy.gov (indexed) [DOE]

    ... Active Capital Project Portfolio Project Title Site Original CD-2 TPC Current TPC EAC Forecast CD-4 Contract Type LINE ITEM Salt Waste Processing Facility (SWPF) SRS 900M 1.3B ...

  7. Lessons Learned | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Within 90 days of Critical Decision (CD-4) approval-- Lessons learned from project execution and facility start-up All PMLLs submitted to OAPM are filed in PARS II under their ...

  8. Fermilab Map Legend

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Area - C-4 Proton Assembly - C-4 PS2 - D-3 PS3 - C-4 PS4, PE3 - C-4 PS5 - C-4 PSI, PSO - D-3 PW2 - D-3 PW3 - D-3 PW4 - D-3 PW6 - C-4 R Ramsey Auditorium - E-2 Roads & Grounds...

  9. Intrathymic radioresistant stem cells follow an IL-2/IL-2R pathway during thymic regeneration after sublethal irradiation

    SciTech Connect (OSTI)

    Zuniga-Pfluecker, J.C.K.; Kruisbeek, A.M. )

    1990-05-15

    Sublethally irradiated mice undergo thymic regeneration which follows a phenotypic pattern of events similar to that observed during normal fetal development. Thymic regeneration after irradiation is the product of a limited pool of intrathymic radioresistant stem cells undergoing simultaneous differentiation. We show that in this model of T cell development, thymic regeneration follows a pathway in which the IL-2R is transiently expressed on CD4-/CD8- cells. IL-2R expression occurred during the exponential growth period of thymic regeneration, and IL-2R blocking prevented this explosive growth. Flow cytometry analysis revealed that the IL-2R blockade affected primarily the development of the immature CD3-/CD4-/CD8- (triple negative) cells and their ability to generate CD3+/CD4+/CD8+ or CD3+/CD4+/CD8- and CD3+/CD4-/CD8+ thymocytes. Thus, our findings demonstrate that blocking of the IL-2R resulted in an arrest in proliferation and differentiation by intrathymic radioresistant stem cells, indicating that the IL-2/IL-2R pathway is necessary for the expansion of immature triple negative T cells.

  10. Strengthening Line Management Oversight and Federal Monitoring of Nuclear Facilities

    Office of Environmental Management (EM)

    1 -- Nuclear Safety Basis Program Review Overview and Management Oversight February 2015 i Standard Review Plan Volume 1 Nuclear Safety Basis Program Review Overview and Management Oversight Facility Life Cycle Applicability CD-1 CD-2 CD-3 CD-4 Operations and Transitions Decommissioning & Environmental Restoration February 2015 ii Table of Contents Acronyms ................................................................................................................................... iii

  11. Strengthening Line Management Oversight and Federal Monitoring of Nuclear Facilities

    Office of Environmental Management (EM)

    Strengthening Line Management Oversight and Federal Monitoring of Nuclear Facilities Standard Review Plan Volume 2 -- Nuclear Safety Basis Program Review During Design February 2015 i Standard Review Plan Volume 2 Nuclear Safety Basis Program Review during Design Facility Life Cycle Applicability CD-1 CD-2 CD-3 CD-4 Operations and Transitions Decommissioning & Environmental Restoration February 2015 ii Table of Contents Acronyms

  12. Strengthening Line Management Oversight and Federal Monitoring of Nuclear Facilities

    Office of Environmental Management (EM)

    Volume 3 - Nuclear Safety Basis Program Review During Facility Operations and Transitions February 2015 i Standard Review Plan Volume 3 Nuclear Safety Basis Program Review during Facility Operations and Transitions Facility Life Cycle Applicability CD-1 CD-2 CD-3 CD-4 Operations and Transitions Decommissioning & Environmental Restoration February 2015 ii Table of Contents Acronyms

  13. Strengthening Line Management Oversight and Federal Monitoring of Nuclear Facilities

    Office of Environmental Management (EM)

    5 - Nuclear Safety Basis Program Review of TSRs, USQs and SERs February 2015 i Standard Review Plan Volume 5 Nuclear Safety Basis Program Review of TSRs, USQs and SERs Facility Life Cycle Applicability CD-1 CD-2 CD-3 CD-4 Operations and Transitions Decommissioning & Environmental Restoration February 2015 ii Table of Contents Acronyms ................................................................................................................................... iii Introduction

  14. Functional and genomic analyses of FOXP3-transduced Jurkat-T cells as regulatory T (Treg)-like cells

    SciTech Connect (OSTI)

    Kim, Joon-Young Kim, Han-Jong; Hurt, Elaine M.; Chen, Xin; Howard, O.M. Zack; Farrar, William L.

    2007-10-12

    FOXP3, a forkhead transcription factor is essential for the development and function of CD4{sup +}CD25{sup +} regulatory T cells (Tregs). In contrast to conversion of murine naive T cells to Tregs by transduction of Foxp3, it is controversial whether ectopic expression of FOXP3 in human CD4{sup +} T cells is sufficient for acquisition of suppressive activity. Here, we show that retroviral transduction of FOXP3 induces a Treg phenotype in human leukemic CD4{sup +} Jurkat-T cells, evidenced by increased expression of Treg-associated cell surface markers as well as inhibition of cytokine production. Furthermore, FOXP3-transduced Jurkat-T cells suppress the proliferation of human CD4{sup +}CD25{sup -} T cells. Additionally, DNA microarray analysis identifies Treg-related genes regulated by FOXP3. Our study demonstrates that enforced expression of FOXP3 confers Treg-like properties on Jurkat-T cells, which can be a convenient and efficient Treg-like cell model for further study to identify Treg cell surface markers and target genes regulated by FOXP3.

  15. Comparative evaluation of trimeric envelope glycoproteins derived from subtype C and B HIV-1 R5 isolates

    SciTech Connect (OSTI)

    Srivastava, Indresh K. Kan, Elaine; Sun Yide; Sharma, Victoria A.; Cisto, Jimna; Burke, Brian; Lian Ying; Hilt, Susan; Biron, Zohar; Hartog, Karin; Stamatatos, Leonidas; Cheng, R. Holland; Ulmer, Jeffrey B.; Barnett, Susan W.

    2008-03-15

    We previously reported that an envelope (Env) glycoprotein immunogen (o-gp140{delta}V2SF162) containing a partial deletion in the second variable loop (V2) derived from the R5-tropic HIV-1 isolate SF162 partially protected vaccinated rhesus macaques against pathogenic SHIV{sub SF162P4} virus. Extending our studies to subtype C isolate TV1, we have purified o-gp140{delta}V2TV1 (subtype C {delta}V2 trimer) to homogeneity, performed glycosylation analysis, and determined its ability to bind CD4, as well as a panel of well-characterized neutralizing monoclonal antibodies (mAb). In general, critical epitopes are preserved on the subtype C {delta}V2 trimer; however, we did not observe significant binding for the b12 mAb. The molecular mass of subtype C {delta}V2 trimer was found to be 450 kDa, and the hydrodynamic radius was found to be 10.87 nm. Our data suggest that subtype C {delta}V2 trimer binds to CD4 with an affinity comparable to o-gp140{delta}V2SF162 (subtype B {delta}V2 trimer). Using isothermal titration calorimetric (ITC) analysis, we demonstrated that all three CD4 binding sites (CD4-BS) in both subtype C and B trimers are exposed and accessible. However, compared to subtype B trimer, the three CD4-BS in subtype C trimer have different affinities for CD4, suggesting a cooperativity of CD4 binding in subtype C trimer but not in subtype B trimer. Negative staining electron microscopy of the subtype C {delta}V2 trimer has demonstrated that it is in fact a trimer. These results highlight the importance of studying subtype C Env, and also of developing appropriate subtype C-specific reagents that may be used for better immunological characterization of subtype C Env for developing an AIDS vaccine.

  16. Pb exposure attenuates hypersensitivity in vivo by increasing regulatory T cells

    SciTech Connect (OSTI)

    Fang, Liang; Zhao, Fang; Shen, Xuefeng; Ouyang, Weiming; Liu, Xinqin; Xu, Yan; Yu, Tao; Jin, Boquan; Chen, Jingyuan; Luo, Wenjing

    2012-12-01

    Pb is a common environmental pollutant affecting various organs. Exposure of the immune system to Pb leads to immunosuppression or immunodysregulation. Although previous studies showed that Pb exposure can modulate the function of helper T cells, Pb immunotoxicity remains incompletely understood. In this study, we investigated the effect of Pb exposure on T cell development, and the underlying mechanism of Pb-induced suppression of the delayed-type hypersensitivity (DTH) response in vivo. SpragueDawley rats were exposed to 300 ppm Pb-acetate solution via the drinking water for six weeks, and we found that Pb exposure significantly increased Pb concentrations in the blood by 4.2-fold (p < 0.05) as compared to those in the control rats. In Pb-exposed rats, the amount of thymic CD4{sup +}CD8{sup ?} and peripheral CD4{sup +} T cells was significantly reduced, whereas, CD8{sup +} population was not affected. In contrast to conventional CD4{sup +} T cells, Foxp3{sup +} regulatory T cells (Tregs) were increased in both the thymus and peripheral lymphoid organs of Pb-exposed rats. In line with the increase of Tregs, the DTH response of Pb-exposed rats was markedly suppressed. Depletion of Tregs reversed the suppression of DTH response by Pb-exposed CD4{sup +} T cells in an adoptive transfer model, suggesting a critical role of the increased Tregs in suppressing the DTH response. Collectively, this study revealed that Pb-exposure may upregulate Tregs, thereby leading to immunosuppression. -- Highlights: ? Pb exposure impaired CD4{sup +} thymic T cell development. ? Peripheral T lymphocytes were reduced following Pb exposure. ? Pb exposure increases thymic and peripheral Treg cells in rats. ? Tregs played a critical role in Pb-exposure-induced immune suppression.

  17. Search for: All records | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    ... Plummer, D. (4) Bergmann, D (3) Cameron-Smith, P (3) Josse, B (3) Lamarque, J F (3) Plummer, D (3) Zeng, G (3) Bergmann, D. (2) Cameron-Smith, P. (2) Dentener, F (2) Doherty, R (2) ...

  18. Method to Exhaust Fusion-Product Tritons and Alpha Particles Rapidly from

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    D-3He Fueled FRC Fusion Reactors | Princeton Plasma Physics Lab Exhaust Fusion-Product Tritons and Alpha Particles Rapidly from D-3He Fueled FRC Fusion Reactors A method is described how to reduce neutron production from small D-3He burning FRC fusion reactors, thereby reducing the shielding required and allowing safer operation and less maintenance. No.: M-898

  19. Curcumin blocks interleukin (IL)-2 signaling in T-lymphocytes by inhibiting IL-2 synthesis, CD25 expression, and IL-2 receptor signaling

    SciTech Connect (OSTI)

    Forward, Nicholas A.; Conrad, David M.; Power Coombs, Melanie R.; Doucette, Carolyn D.; Furlong, Suzanne J.; Lin, Tong-Jun; Department of Pediatrics, Dalhousie University, Halifax, Nova Scotia ; Hoskin, David W.

    2011-04-22

    Highlights: {yields} Curcumin inhibits CD4{sup +} T-lymphocyte proliferation. {yields} Curcumin inhibits interleukin-2 (IL-2) synthesis and CD25 expression by CD4{sup +} T-lymphocytes. {yields} Curcumin interferes with IL-2 receptor signaling by inhibiting JAK3 and STAT5 phosphorylation. {yields} IL-2-dependent regulatory T-lymphocyte function and Foxp3 expression is downregulated by curcumin. -- Abstract: Curcumin (diferulomethane) is the principal curcuminoid in the spice tumeric and a potent inhibitor of activation-induced T-lymphocyte proliferation; however, the molecular basis of this immunosuppressive effect has not been well studied. Here we show that micromolar concentrations of curcumin inhibited DNA synthesis by mouse CD4{sup +} T-lymphocytes, as well as interleukin-2 (IL-2) and CD25 ({alpha} chain of the high affinity IL-2 receptor) expression in response to antibody-mediated cross-linking of CD3 and CD28. Curcumin acted downstream of protein kinase C activation and intracellular Ca{sup 2+} release to inhibit I{kappa}B phosphorylation, which is required for nuclear translocation of the transcription factor NF{kappa}B. In addition, IL-2-dependent DNA synthesis by mouse CTLL-2 cells, but not constitutive CD25 expression, was impaired in the presence of curcumin, which demonstrated an inhibitory effect on IL-2 receptor (IL-2R) signaling. IL-2-induced phosphorylation of STAT5A and JAK3, but not JAK1, was diminished in the presence of curcumin, indicating inhibition of critical proximal events in IL-2R signaling. In line with the inhibitory action of curcumin on IL-2R signaling, pretreatment of CD4{sup +}CD25{sup +} regulatory T-cells with curcumin downregulated suppressor function, as well as forkhead box p3 (Foxp3) expression. We conclude that curcumin inhibits IL-2 signaling by reducing available IL-2 and high affinity IL-2R, as well as interfering with IL-2R signaling.

  20. Construction Readiness RM | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Readiness RM Construction Readiness RM The authorization to proceed with construction of a new facility is given at the CD-3 phase of the project management cycle, after completion of the final design. Between CD-3 and CD-4 stages of the project, procurement and construction and/or assembly of facility structures, systems and equipment is conducted. These activities can present significant hazards to workers and involve a complex set of events that must be carefully planned and sequenced.

  1. Strengthening Line Management Oversight and Federal Monitoring of Nuclear Facilities

    Office of Environmental Management (EM)

    Strengthening Line Management Oversight and Federal Monitoring of Nuclear Facilities Standard Review Plan Volume 4 - Nuclear Safety Basis Program Review During Facility Decommissioning and Environmental Restoration February 2015 i Standard Review Plan Volume 4 Nuclear Safety Basis Program Review during Facility Decommissioning and Environmental Restoration Facility Life Cycle Applicability CD-1 CD-2 CD-3 CD-4 Operations and Transitions Decommissioning & Environmental Restoration February

  2. Preparation for Facility Operations RM | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Preparation for Facility Operations RM Preparation for Facility Operations RM The objective of this Standard Review Plan (SRP) on Preparation for Facility Operations is to provide consistency guidance to evaluate the effectiveness of the final project closure of major construction projects for transition from Critical Decision-4 (CD-4) to facility operations. Preparation for Facility Operations RM (356.48 KB) More Documents & Publications Code of Record Standard Review Plan (SRP) Standard

  3. Structural analysis of a novel rabbit monoclonal antibody R53 targeting an epitope in HIV-1 gp120 C4 region critical for receptor and co-receptor binding

    SciTech Connect (OSTI)

    Pan, Ruimin; Chen, Yuxin; Vaine, Michael; Hu, Guangnan; Wang, Shixia; Lu, Shan; Kong, Xiang -Peng

    2015-07-15

    The fourth conserved region (C4) in the HIV-1 envelope glycoprotein (Env) gp120 is a structural element that is important for its function, as it binds to both the receptor CD4 and the co-receptor CCR5/CXCR4. It has long been known that this region is highly immunogenic and that it harbors B-cell as well as T-cell epitopes. It is the target of a number of antibodies in animal studies, which are called CD4-blockers. However, the mechanism by which the virus shields itself from such antibody responses is not known. Here, we determined the crystal structure of R53 in complex with its epitope peptide using a novel anti-C4 rabbit monoclonal antibody R53. Our data show that although the epitope of R53 covers a highly conserved sequence 433AMYAPPI439, it is not available in the gp120 trimer and in the CD4-bound conformation. Our results suggest a masking mechanism to explain how HIV-1 protects this critical region from the human immune system.

  4. Structural analysis of a novel rabbit monoclonal antibody R53 targeting an epitope in HIV-1 gp120 C4 region critical for receptor and co-receptor binding

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Pan, Ruimin; Chen, Yuxin; Vaine, Michael; Hu, Guangnan; Wang, Shixia; Lu, Shan; Kong, Xiang -Peng

    2015-07-15

    The fourth conserved region (C4) in the HIV-1 envelope glycoprotein (Env) gp120 is a structural element that is important for its function, as it binds to both the receptor CD4 and the co-receptor CCR5/CXCR4. It has long been known that this region is highly immunogenic and that it harbors B-cell as well as T-cell epitopes. It is the target of a number of antibodies in animal studies, which are called CD4-blockers. However, the mechanism by which the virus shields itself from such antibody responses is not known. Here, we determined the crystal structure of R53 in complex with its epitopemore » peptide using a novel anti-C4 rabbit monoclonal antibody R53. Our data show that although the epitope of R53 covers a highly conserved sequence 433AMYAPPI439, it is not available in the gp120 trimer and in the CD4-bound conformation. Our results suggest a masking mechanism to explain how HIV-1 protects this critical region from the human immune system.« less

  5. Search for: All records | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    (1) condensed matter physics, superconductivity and superfluidity (1) copper (1) copper compounds (1) Filter by Author Pallecchi, I. (5) Marre, D. (3) Cantoni, Claudia ...

  6. Detecting regular sound changes in linguistics as events of concerted...

    Office of Scientific and Technical Information (OSTI)

    other evolving group. less Authors: Hruschka, Daniel J. 1 ; Branford, Simon 2 ; Smith, Eric D. 3 ; Wilkins, Jon 4 ; Meade, Andrew 2 ; Pagel, Mark 5 ; Bhattacharya,...

  7. Modcopter: Prompt, Precise Aerial Sample Collection Using Unmanned...

    Office of Scientific and Technical Information (OSTI)

    Title: Modcopter: Prompt, Precise Aerial Sample Collection Using Unmanned Systems Authors: Curtis, Aaron 1 ; Elliott, James 2 ; Ronquest, Michael 3 ; Mascarenas, David D. 3 ...

  8. Search for: All records | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    Martin, Christopher (5) Martin, Christopher D. (5) Seibert, Mark (5) Johnson, Benjamin D. (3) Schiminovich, David (3) Charlot, Stephane (2) Heckman, Timothy M. (2) Martins, ...

  9. Search for: All records | DOE PAGES

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    Saxena, Avadh (15) Khare, Avinash (4) Lin, Shi-Zeng (4) Batista, Cristian D. (3) Cooper, Fred (2) Deffner, Sebastian (2) Kevrekidis, Panayotis G. (2) Reichhardt, Charles (2)...

  10. A Hierarchical Evaluation of Regional Climate Simulations (Journal...

    Office of Scientific and Technical Information (OSTI)

    Authors: Leung, Ruby 1 ; Ringler, Todd 2 ; Collins, William D 3 ; Taylor, Mark 4 ; Ashfaq, Moetasim 5 + Show Author Affiliations Pacific Northwest National Laboratory ...

  11. Search for: All records | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    ... Zaug, J M (8) Bastea, S (5) Browning, N D (3) Grant, C D (3) Reed, B W (3) Armstrong, M. R. (2) Campbell, G H (2) Fried, L E (2) Kim, J S (2) King, W E (2) Torralva, B R (2) ...

  12. Argonne_map

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ROADS & PARKING BUILDINGS 603 1 2 3 4 5 A B C D E F G 360 - E4 361 - E4 362 - E3 363 - E4 366 - E4 369 - E4 370 - E4 371 - E4 376 - E4 399 - E4 600 Area 600 - E3 617 - E3 800 Area 891 - B2 900 Area 950 - G3 951 - G2 952 - G2 953 - G2 East Area 046 - F2 091 - G3 100 Area 108 - E2 145 - F2 146 - F2 200 Area 200 - C2 201 - D2 202 - D2 203 - D2 204 - E2 205 - D2, D3 300 Area 302 - D3 306 - D4 308 - D3 309 - D3 206 - C2 208 - C2 211 - C2 212 - D2 213 - D2 214 - D3 216 - D2 314 - D4 315 - D4 316 -

  13. Bioinformatic analysis of neurotropic HIV envelope sequences identifies polymorphisms in the gp120 bridging sheet that increase macrophage-tropism through enhanced interactions with CCR5

    SciTech Connect (OSTI)

    Mefford, Megan E.; Kunstman, Kevin; Wolinsky, Steven M.; Gabuzda, Dana

    2015-07-15

    Macrophages express low levels of the CD4 receptor compared to T-cells. Macrophage-tropic HIV strains replicating in brain of untreated patients with HIV-associated dementia (HAD) express Envs that are adapted to overcome this restriction through mechanisms that are poorly understood. Here, bioinformatic analysis of env sequence datasets together with functional studies identified polymorphisms in the β3 strand of the HIV gp120 bridging sheet that increase M-tropism. D197, which results in loss of an N-glycan located near the HIV Env trimer apex, was detected in brain in some HAD patients, while position 200 was estimated to be under positive selection. D197 and T/V200 increased fusion and infection of cells expressing low CD4 by enhancing gp120 binding to CCR5. These results identify polymorphisms in the HIV gp120 bridging sheet that overcome the restriction to macrophage infection imposed by low CD4 through enhanced gp120–CCR5 interactions, thereby promoting infection of brain and other macrophage-rich tissues. - Highlights: • We analyze HIV Env sequences and identify amino acids in beta 3 of the gp120 bridging sheet that enhance macrophage tropism. • These amino acids at positions 197 and 200 are present in brain of some patients with HIV-associated dementia. • D197 results in loss of a glycan near the HIV Env trimer apex, which may increase exposure of V3. • These variants may promote infection of macrophages in the brain by enhancing gp120–CCR5 interactions.

  14. Inhibition of HIV-1 entry by the tricyclic coumarin GUT-70 through the modification of membrane fluidity

    SciTech Connect (OSTI)

    Matsuda, Kouki; Hattori, Shinichiro; Kariya, Ryusho; Komizu, Yuji; Kudo, Eriko; Goto, Hiroki; Taura, Manabu; Ueoka, Ryuichi; Kimura, Shinya; Okada, Seiji

    2015-02-13

    Membrane fusion between host cells and HIV-1 is the initial step in HIV-1 infection, and plasma membrane fluidity strongly influences infectivity. In the present study, we demonstrated that GUT-70, a natural product derived from Calophyllum brasiliense, stabilized plasma membrane fluidity, inhibited HIV-1 entry, and down-regulated the expression of CD4, CCR5, and CXCR4. Since GUT-70 also had an inhibitory effect on viral replication through the inhibition of NF-κB, it is expected to be used as a dual functional and viral mutation resistant reagent. Thus, these unique properties of GUT-70 enable the development of novel therapeutic agents against HIV-1 infection.

  15. Proposals and Design Reports

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Proposals and Design Reports Proposals and Design Reports Proposal (1/2/04) Proposal Addendum (29/3/04) Physics Case and Detector Technology Report (12/04) Technical Design Report (7/06) Reviews DOE CD-4 DOE 2008 DOE CD-3b CD-3b Readiness DOE CD-1/2/3a CD-2/3a Readiness CD-1 Readiness PAC Report (4/05) Approvals FNAL PAC Approval: April 15, 2004 DOE Critical Decision 1,2,3a (Performance Baseline, Construction Start) Approval: March 30, 2007 DOE Critical Decision 4 (Project Completion) Approval:

  16. approved_list

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    11OCT99 Exp # Spokesperson Title Days 789-2 Clark Exotic Shapes in 106Cd 4 806-2 Kondev Probing the Interplay Between Intrinsic and Collective Motion at the Extremes of Spin, Spin Projection and Seniority 5 821X Hofman In-Beam Test of LEPPEX Fission Chamber and Detectors 3 822 Kwok Patterning of Columnar Defects: Fabrication of Vortex Ratchet System for Manipulating Trapped Flux in High Temperature Superconductors 2 823 Svensson Lifetimes in the (Super)-Deformed (f7/2)4 Intruder Band in the N =

  17. Prenatal cadmium exposure produces persistent changes to thymus and spleen cell phenotypic repertoire as well as the acquired immune response

    SciTech Connect (OSTI)

    Holskov, Ida; Elliott, Meenal; Hanson, Miranda L.; Schafer, Rosana [Department of Microbiology, Immunology and Cell Biology, West Virginia University School of Medicine, Morgantown, WV 26506 (United States)] [Department of Microbiology, Immunology and Cell Biology, West Virginia University School of Medicine, Morgantown, WV 26506 (United States); Barnett, John B., E-mail: jbarnett@hsc.wvu.edu [Department of Microbiology, Immunology and Cell Biology, West Virginia University School of Medicine, Morgantown, WV 26506 (United States); Mary Babb Randolph Cancer Center, West Virginia University School of Medicine, Morgantown, WV 26506 (United States)

    2012-12-01

    Cadmium (Cd) is a common environmental contaminant. Adult exposure to Cd alters the immune system, however, there are limited studies on the effects of prenatal exposure to Cd. Pregnant C57Bl/6 mice were exposed to an environmentally relevant dose of CdCl{sub 2} (10 ppm) and the effects on the immune system of the offspring were assessed at 20 weeks of age. Prenatal Cd exposure caused an increase in the percent of CD4{sup ?}CD8{sup ?}CD44{sup +}CD25{sup ?} (DN1) thymocytes in both sexes and a decrease in the percent of CD4{sup ?}CD8{sup ?}CD44{sup ?}CD25{sup +} (DN3) thymocytes in females. Females had an increase in the percent of splenic CD4{sup +} T cells, CD8{sup +} T cells, and CD45R/B220{sup +} B cells and a decrease in the percent of NK cells and granulocytes (Gr-1{sup +}). Males had an increase in the percent of splenic CD4{sup +} T cells and CD45R/B220{sup +} B cells and a decrease in the percent of CD8{sup +} T cells, NK cells, and granulocytes. The percentage of neutrophils and myeloid-derived suppressor cells were reduced in both sexes. The percent of splenic nTreg cells was decreased in all Cd-exposed offspring. Cd-exposed offspring were immunized with a streptococcal vaccine and the antibody response was determined. PC-specific serum antibody titers were decreased in Cd exposed female offspring but increased in the males. PspA-specific serum IgG titers were increased in both females and males compared to control animals. Females had a decrease in PspA-specific serum IgM antibody titers. Females and males had a decrease in the number of splenic anti-PspA antibody-secreting cells when standardized to the number of B cells. These findings demonstrate that very low levels of Cd exposure during gestation can result in long term sex-specific alterations on the immune system of the offspring. -- Highlights: ? Prenatal exposure to cadmium alters the immune system of 20 week old offspring. ? The percentage of DN1 and DN3 thymocytes was changed. ? Males and

  18. J-(_5A.-

    Office of Legacy Management (LM)

    014 !-PI-j I ii f , i- .:-I' I :c ( Ii' iUC ./<: ./ /i' J-(_5A.- - n -Il&.L-, ($1 (.' j / +P ' , Cd , - $' -- 4 . NATIONAL LEAD COMPAN\r OF OHIO P. 0. BOX 158 MT. HEALTHY STATION CINCINNATI 31. OHIO Letter Subecmtract # s-19 Dated: October Ql,l952 Under Prime Contract AT(~O-l-)-1.1.56 ~0: Mr. A. M. Kinney, President Processes Research, Inc. 2905 Vernon Place Cincinnati 19, Ohio Gentlemen: 1. This letter, subject to your written acceptance and the approval of the Atmic Energy Comnission

  19. Estimation and modeling of coal pore accessibility using small angle neutron scattering

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Zhang, Rui; Liu, Shimin; Bahadur, Jitendra; Elsworth, Derek; Melnichenko, Yuri; He, Lilin; Wang, Yi

    2015-09-04

    Gas diffusion in coal is controlled by nano-structure of the pores. The interconnectivity of pores not only determines the dynamics of gas transport in the coal matrix but also influences the mechanical strength. In this study, small angle neutron scattering (SANS) was employed to quantify pore accessibility for two coal samples, one of sub-bituminous rank and the other of anthracite rank. Moreover, a theoretical pore accessibility model was proposed based on scattering intensities under both vacuum and zero average contrast (ZAC) conditions. Our results show that scattering intensity decreases with increasing gas pressure using deuterated methane (CD4) at low Qmore » values for both coals. Pores smaller than 40 nm in radius are less accessible for anthracite than sub-bituminous coal. On the contrary, when the pore radius is larger than 40 nm, the pore accessibility of anthracite becomes larger than that of sub-bituminous coal. Only 20% of pores are accessible to CD4 for anthracite and 37% for sub-bituminous coal, where the pore radius is 16 nm. For these two coals, pore accessibility and pore radius follows a power-law relationship.« less

  20. Effects of Compactification in D-brane Inflation (Journal Article...

    Office of Scientific and Technical Information (OSTI)

    Effects of Compactification in D-brane Inflation Citation Details In-Document Search Title: Effects of Compactification in D-brane Inflation In D3-brane inflation, the inflaton ...

  1. Search for: All records | SciTech Connect

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    ... Giroletti, M. INAF Istituto di Radioastronomia, via Gobetti 101, I-40129 Bologna (Italy) (4) Chavushyan, V. (3) Fragos, T. (3) Gasparrini, D. (3) Kim, D.-W. (3) Landoni, M. (3) ...

  2. The microscopic structure of charge density waves in underdoped...

    Office of Scientific and Technical Information (OSTI)

    Authors: E. M. Forgan ; Huecker, M. 1 ; Blackburn, E. 2 ; Holmes, A. T. 2 ; Briffa, A. K. R. 2 ; Chang, J. 2 ; Bouchenoire, L. 3 ; Brown, S. D. 3 ; Liang, Ruixing 4 ...

  3. RAPID/Roadmap/3-OR-d | Open Energy Information

    Open Energy Info (EERE)

    potential, geologic and geophysical test data, and other information of a factual nature. See OAR 141-075-0525. 3-OR-d.3 - Notice of bidding The Oregon Department of State...

  4. Search for: microelectromechanical OR MEMS | DOE PAGES

    Office of Scientific and Technical Information (OSTI)

    Lee, S. W. (3) Magierski, Piotr (3) Monroe, Matthew E. (3) Olsson, Roy H. (3) Smith, Adam W. (3) Smith, Richard D. (3) Wang, H. (3) Wlazowski, Gabriel (3) Aad, G. (2) Adkins, ...

  5. Validating (d,pγ) as a surrogate for neutron capture (Conference...

    Office of Scientific and Technical Information (OSTI)

    ; Lister, C. J. 11 ; Manning, B. 1 ; O'Donnell, John M. 4 ; Peters, W. A. 12 ; Ross, T. J. 8 ; Scielzo, N. D. 3 ; Seweryniak, D. 7 ; Zhu, S. 7 less + Show ...

  6. NOTICE OF PROPOSED RULEMAKING ON THE INTEGRATED INTERAGENCY PRE...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    The workshop will be held via webinar and conference call. Please register at: 1) Webinar link: https:iser.webex.comiseronstageg.php?MTIDedfa1833d3ad5014f2b91124860aeecc1 2) ...

  7. Search for: All records | SciTech Connect

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    ... Filter by Author Raman, R N (17) Demos, S G (12) Negres, R A (9) Matthews, M J (6) Rubenchik, A M (4) Matthews, D L (3) Pivetti, C D (3) Troppmann, C (3) Carr, C W (2) Elhadj, S ...

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    Cao, Huibo (3) Christianson, A. D. (3) Fernandez-Baca, Jaime A. (3) Matsuda, Masaaki (3) ... M. ; Christianson, A. D. ; Fernandez-Baca, J. A. ; Harriger, L. W. ; et al May 2016 ...

  9. Interlanguage Programming

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    &letter1, &numint1, &numint2, &numfloat1, &numdoub1, &numshor1, &myid, 1); printf(" Hello from proc %d of %d; data values: %s %c %d %d %3.1f %.0f %dn", myid, numprocs,...

  10. Seismic Techniques | Open Energy Information

    Open Energy Info (EERE)

    impedance boundary7 References (Majer, n.d.) "3-D Seismic Methods For Geothermal Reservoir Exploration and Assessment- Summary" 2.0 2.1 2.2 (Dobrin and Savit, 1988)...

  11. Ethanol Ventures | Open Energy Information

    Open Energy Info (EERE)

    Ethanol Ventures Place: London, England, United Kingdom Zip: W1D 3SQ Product: Company aims to deliver at least 378 million litres a year of bioethanol from two Facilities in...

  12. Search for: microelectromechanical OR MEMS | DOE PAGES

    Office of Scientific and Technical Information (OSTI)

    Monroe, Matthew E. (3) Mostepanenko, V. M. (3) Nowadnick, E. A. (3) Olsson, Roy H. (3) Smith, Adam W. (3) Smith, Richard D. (3) Save Results Excel (limit 2000) CSV (limit 5000) XML ...

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    ... Lamarque, J F (4) Zeng, G (4) Zeng, G. (4) Cameron-Smith, P (3) Dentener, F (3) Doherty, R (3) Nagashima, T. (3) Plummer, D (3) Sudo, K. (3) Cameron-Smith, P. (2) Cameron-Smith, ...

  14. Microsoft Word - appxd.doc

    Office of Legacy Management (LM)

    RFSOG Appendix D, Page 1 D.1 Points of Compliance Appendix D, Page 2 D.2 Points of Evaluation Appendix D, Page 3 D.3 Area of Concern Wells, Boundary Wells, and SW018 Appendix D,...

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    ... and Development (NA-22) Office of River Protection (United States) Office of the Secretary ... Meister, A. (3) Milek, B. (3) Ratcliff, B. (3) Schultz, D. (3) Save Results Save this ...

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    ... (7) fermions (6) gauge invariance (6) geometry (6) string theory (6) supersymmetry ... Inclusion of a small number of ovr D3 branes in the resulting warped geometry allows one ...

  17. Microsoft Word - appxd

    Office of Legacy Management (LM)

    the RFSOG Appendix D, Page 1 D.1 Points of Compliance Appendix D, Page 2 D.2 Points of Evaluation Appendix D, Page 3 D.3 Area of Concern Wells and SW018 Appendix D, Page 4 D.4...

  18. EERE's Fiscal Year 2005 Budget in Brief

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    FY 2005 Budget-in-Brief U.S. Department of Energy Energy Efficiency and Renewable Energy www.eere.energy.gov TABLE OF CONTENTS Page Introduction .......................................................................................................................1 Biomass & Biorefinery Systems R&D ...............................................................................3 Building Technologies

  19. Search for: All records | SciTech Connect

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    ... Majumder, Abhijit (5) Paquet, Jean-Franois (5) Schenke, Bjoern (5) Turbide, Simon (5) Moore, Guy D. (3) Shen, Chun (3) Young, Clint (3) Bass, Steffen A. (2) Bourque, Alex (2) ...

  20. Search for: All records | DOE PAGES

    Office of Scientific and Technical Information (OSTI)

    Tai, Yuan-Yen (4) Ahmed, Towfiq (3) Bauer, Eric D. (3) Chia, Elbert E. M. (3) Janoschek, ... S. A. ; Wu, T. ; Panagopoulos, C. ; Chia, Elbert E. M. ; Zhu, Jian-Xin February ...

  1. Method to Increase 3He Supply for Use in Small Clean Fusion Reactors |

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Princeton Plasma Physics Lab Increase 3He Supply for Use in Small Clean Fusion Reactors A method is described how to fuel many small D-3He burning FRC fusion reactors by building a remote D-D burning reactor twin (for each D-3He burning reactor) to breed 3He. Both reactors would employ our novel fusion ash exhaust technique. No.: M-897

  2. Sodium meta-arsenite prevents the development of autoimmune diabetes in NOD mice

    SciTech Connect (OSTI)

    Lee, Y.S.; Kim, D.; Lee, E.K.; Kim, S.; Choi, C.S.; Jun, H.S.

    2015-04-15

    Sodium meta-arsenite (SA) is an orally available arsenic compound. We investigated the effects of SA on the development of autoimmune type 1 diabetes. Female non-obese diabetic (NOD) mice were orally intubated with SA (5 mg/kg/day) from 8 weeks of age for 8 weeks. The cumulative incidence of diabetes was monitored until 30 weeks of age, islet histology was examined, and lymphocytes including T cells, B cells, CD4+ IFN-γ+ cells, CD8+ IFN-γ+ cells, CD4+ IL-4+ cells, and regulatory T cells were analyzed. We also investigated the diabetogenic ability of splenocytes using an adoptive transfer model and the effect of SA on the proliferation, activation, and expression of glucose transporter 1 (Glut1) in splenocytes treated with SA in vitro and splenocytes isolated from SA-treated mice. SA treatment decreased the incidence of diabetes and delayed disease onset. SA treatment reduced the infiltration of immunocytes in islets, and splenocytes from SA-treated mice showed a reduced ability to transfer diabetes. The number of total splenocytes and T cells and both the number and the proportion of CD4+ IFN-γ+ and CD8+ IFN-γ+ T cells in the spleen were significantly reduced in SA-treated NOD mice compared with controls. The number, but not the proportion, of regulatory T cells was decreased in SA-treated NOD mice. Treatment with SA either in vitro or in vivo inhibited proliferation of splenocytes. In addition, the expression of Glut1 and phosphorylated ERK1/2 was decreased by SA treatment. These results suggest that SA reduces proliferation and activation of T cells, thus preventing autoimmune diabetes in NOD mice. - Highlights: • SA prevents the development of diabetes and delays the age of onset in NOD mice. • SA decreases the number but not the proportion of T lymphocytes in NOD mice. • SA reduces IFN-γ-producing T lymphocytes in NOD mice. • SA reduces proliferation and activation of T lymphocytes in vitro and in vivo. • SA reduces the expression of glucose

  3. 12 GeV! | Jefferson Lab

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    GeV! 12 GeV! December 21, 2015 Our upgrade project is called the 12 GeV CEBAF Upgrade Project. At the time CD-4A was achieved, we demonstrated 2.2 GeV per pass. This was 12 GeV! Well, not quite. In fact with more than one pass, we limited ourselves to a little more than 6 GeV with three passes, and to 10.5 GeV with 5.5 passes. It was not felt to be prudent to demand 12 GeV out of the machine immediately after turn on. Operations in the spring of 2015 at high energy, ~10.5 GeV, came to a

  4. Optimal combinations of broadly neutralizing antibodies for prevention and treatments of HIV-1 clade C infection

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Wagh, Kshitij; Bhattacharya, Tanmoy; Williamson, Carolyn; Robles, Alex; Bayne, Madeleine; Garrity, Jetta; Rist, Michael; Rademeyer, Cecilia; Yoon, Hyejin; Lapedes, Alan Scott; et al

    2016-03-30

    In this study, the identification of a new generation of potent broadly neutralizing HIV-1 antibodies (bnAbs) has generated substantial interest in their potential use for the prevention and/or treatment of HIV-1 infection. While combinations of bnAbs targeting distinct epitopes on the viral envelope (Env) will likely be required to overcome the extraordinary diversity of HIV-1, a key outstanding question is which bnAbs, and how many, will be needed to achieve optimal clinical benefit. We assessed the neutralizing activity of 15 bnAbs targeting four distinct epitopes of Env, including the CD4-binding site (CD4bs), the V1/V2-glycan region, the V3-glycan region, and themore » gp41 membrane proximal external region (MPER), against a panel of 200 acute/early clade C HIV-1 Env pseudoviruses. A mathematical model was developed that predicted neutralization by a subset of experimentally evaluated bnAb combinations with high accuracy. Using this model, we performed a comprehensive and systematic comparison of the predicted neutralizing activity of over 1,600 possible double, triple, and quadruple bnAb combinations. The most promising bnAb combinations were identified based not only on breadth and potency of neutralization, but also other relevant measures, such as the extent of complete neutralization and instantaneous inhibitory potential (IIP). By this set of criteria, triple and quadruple combinations of bnAbs were identified that were significantly more effective than the best double combinations, and further improved the probability of having multiple bnAbs simultaneously active against a given virus, a requirement that may be critical for countering escape in vivo. These results provide a rationale for advancing bnAb combinations with the best in vitro predictors of success into clinical trials for both the prevention and treatment of HIV-1 infection.« less

  5. Structural Analysis of a Highly Glycosylated and Unliganded gp120-Based Antigen Using Mass Spectrometry

    SciTech Connect (OSTI)

    L Wang; Y Qin; S Ilchenko; J Bohon; W Shi; M Cho; K Takamoto; M Chance

    2011-12-31

    Structural characterization of the HIV-1 envelope protein gp120 is very important for providing an understanding of the protein's immunogenicity and its binding to cell receptors. So far, the crystallographic structure of gp120 with an intact V3 loop (in the absence of a CD4 coreceptor or antibody) has not been determined. The third variable region (V3) of the gp120 is immunodominant and contains glycosylation signatures that are essential for coreceptor binding and entry of the virus into T-cells. In this study, we characterized the structure of the outer domain of gp120 with an intact V3 loop (gp120-OD8) purified from Drosophila S2 cells utilizing mass spectrometry-based approaches. We mapped the glycosylation sites and calculated the glycosylation occupancy of gp120-OD8; 11 sites from 15 glycosylation motifs were determined as having high-mannose or hybrid glycosylation structures. The specific glycan moieties of nine glycosylation sites from eight unique glycopeptides were determined by a combination of ECD and CID MS approaches. Hydroxyl radical-mediated protein footprinting coupled with mass spectrometry analysis was employed to provide detailed information about protein structure of gp120-OD8 by directly identifying accessible and hydroxyl radical-reactive side chain residues. Comparison of gp120-OD8 experimental footprinting data with a homology model derived from the ligated CD4-gp120-OD8 crystal structure revealed a flexible V3 loop structure in which the V3 tip may provide contacts with the rest of the protein while residues in the V3 base remain solvent accessible. In addition, the data illustrate interactions between specific sugar moieties and amino acid side chains potentially important to the gp120-OD8 structure.

  6. Effects of Respiratory Motion on Passively Scattered Proton Therapy Versus Intensity Modulated Photon Therapy for Stage III Lung Cancer: Are Proton Plans More Sensitive to Breathing Motion?

    SciTech Connect (OSTI)

    Matney, Jason; Park, Peter C. [Department of Radiation Physics, University of Texas MD Anderson Cancer Center, Houston, Texas (United States); The University of Texas Graduate School of Biomedical Sciences, Houston, Texas (United States); Bluett, Jaques [Department of Radiation Oncology, University of Texas MD Anderson Cancer Center, Houston, Texas (United States); Chen, Yi Pei [Department of Radiation Physics, University of Texas MD Anderson Cancer Center, Houston, Texas (United States); The University of Texas Graduate School of Biomedical Sciences, Houston, Texas (United States); Liu, Wei; Court, Laurence E. [Department of Radiation Physics, University of Texas MD Anderson Cancer Center, Houston, Texas (United States); Liao, Zhongxing [Department of Radiation Oncology, University of Texas MD Anderson Cancer Center, Houston, Texas (United States); Li, Heng [Department of Radiation Physics, University of Texas MD Anderson Cancer Center, Houston, Texas (United States); Mohan, Radhe, E-mail: rmohan@mdanderson.org [Department of Radiation Physics, University of Texas MD Anderson Cancer Center, Houston, Texas (United States)

    2013-11-01

    Purpose: To quantify and compare the effects of respiratory motion on paired passively scattered proton therapy (PSPT) and intensity modulated photon therapy (IMRT) plans; and to establish the relationship between the magnitude of tumor motion and the respiratory-induced dose difference for both modalities. Methods and Materials: In a randomized clinical trial comparing PSPT and IMRT, radiation therapy plans have been designed according to common planning protocols. Four-dimensional (4D) dose was computed for PSPT and IMRT plans for a patient cohort with respiratory motion ranging from 3 to 17 mm. Image registration and dose accumulation were performed using grayscale-based deformable image registration algorithms. The dosevolume histogram (DVH) differences (4D-3D [3D = 3-dimensional]) were compared for PSPT and IMRT. Changes in 4D-3D dose were correlated to the magnitude of tumor respiratory motion. Results: The average 4D-3D dose to 95% of the internal target volume was close to zero, with 19 of 20 patients within 1% of prescribed dose for both modalities. The mean 4D-3D between the 2 modalities was not statistically significant (P<.05) for all dosevolume histogram indices (mean SD) except the lung V5 (PSPT: +1.1% 0.9%; IMRT: +0.4% 1.2%) and maximum cord dose (PSPT: +1.5 2.9 Gy; IMRT: 0.0 0.2 Gy). Changes in 4D-3D dose were correlated to tumor motion for only 2 indices: dose to 95% planning target volume, and heterogeneity index. Conclusions: With our current margin formalisms, target coverage was maintained in the presence of respiratory motion up to 17 mm for both PSPT and IMRT. Only 2 of 11 4D-3D indices (lung V5 and spinal cord maximum) were statistically distinguishable between PSPT and IMRT, contrary to the notion that proton therapy will be more susceptible to respiratory motion. Because of the lack of strong correlations with 4D-3D dose differences in PSPT and IMRT, the extent of tumor motion was not an adequate predictor of potential

  7. Effect of long-term culturing on the potential of mouse embryonic stem cells for in vitro and in vivo development

    SciTech Connect (OSTI)

    Mitalipov, Sh.M.; Mitalipova, M.M.; Ivanov, V.I.

    1994-11-01

    We performed comparative analysis of in vitro and in vivo pluripotency for two clones of ES-D3 cells subjected to different number of passages after the beginning of subcloning. Both clones of ES cells produced characteristically shaped colonies and embryoid bodies during culturing in suspension. High activity of alkaline phosphatase was demonstrated in ES cells by cytochemical staining. The proportion of aneuploid ES cells increased with the increase in the number of passages, as shown by karyotyping. Experiments on producing chimeric mice using ES cells have shown that clone D3W (passage 17) exceeds clone D3M (passage 42) both in terms of chimera proportion among the offspring and in terms of the extent of coat chimerism (proportion of agouti coat color (ES component) in the coat of chimeras). 26 refs., 4 figs., 3 tabs.

  8. Spin-orbit coupling controlled ground state in Sr2ScOsO6

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Taylor, A. E.; Morrow, R.; Fishman, R. S.; Calder, S.; Kolesnikov, A. I.; Lumsden, M. D.; Woodward, P. M.; Christianson, A. D.

    2016-06-27

    In this paper, we report neutron scattering experiments which reveal a large spin gap in the magnetic excitation spectrum of weakly-monoclinic double perovskite Sr2ScOsO6. The spin gap is demonstrative of appreciable spin-orbit-induced anisotropy, despite nominally orbitally-quenched 5d3Os5+ ions. The system is successfully modeled including nearest neighbor interactions in a Heisenberg Hamiltonian with exchange anisotropy. We find that the presence of the spin-orbit-induced anisotropy is essential for the realization of the type I antiferromagnetic ground state. Finally, this demonstrates that physics beyond the LS or JJ coupling limits plays an active role in determining the collective properties of 4d3 and 5d3more » systems and that theoretical treatments must include spin-orbit coupling.« less

  9. Gauge theories on hyperbolic spaces and dual wormhole instabilities

    SciTech Connect (OSTI)

    Buchel, Alex

    2004-09-15

    We study supergravity duals of strongly coupled four-dimensional gauge theories formulated on compact quotients of hyperbolic spaces. The resulting background geometries are represented by Euclidean wormholes, which complicate establishing the precise gauge theory/string theory correspondence dictionary. These backgrounds suffer from the nonperturbative instabilities arising from the D3D3-bar pair-production in the background four-form potential. We discuss conditions for suppressing this Schwingerlike instability. We find that Euclidean wormholes arising in this construction develop a naked singularity before they can be stabilized.

  10. Closure certification report for the Bear Creek burial grounds B area and walk-in pits at the Oak Ridge Y-12 Plant, Oak Ridge, Tennessee

    SciTech Connect (OSTI)

    Not Available

    1994-06-01

    On July 5, 1993, the revised RCRA Closure Plan for the Bear Creek Burial Grounds B Area and Walk-In Pits at the Oak Ridge Y-12 Plant, Oak Ridge, Tennessee, DOE/OR/01-1100&D3 and Y/ER-53&D3, was approved by the Tennessee Department of Environment and Conservation (TDEC). The closure activities described in that closure plan have been performed. The purpose of this document is to summarize the closure activities for B Area and Walk-In Pits (WIPs), including placement of the Kerr Hollow Quarry debris at the WIPs.

  11. First Observations of Nonhydrodynamic Mix at the Fuel-Shell Interface in Shock-Driven Inertial Confinement Implosions

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Rinderknecht, H. G.; Sio, H.; Li, C. K.; Zylstra, A. B.; Rosenberg, M. J.; Amendt, P.; Delettrez, J.; Bellei, C.; Frenje, J. A.; Gatu Johnson, M.; et al

    2014-04-01

    A strong nonhydrodynamic mechanism generating atomic fuel-shell mix has been observed in strongly shocked inertial confinement fusion implosions of thin deuterated-plastic shells filled with 3He gas. These implosions were found to produce D3He-proton shock yields comparable to implosions of identical shells filled with a hydroequivalent 50:50 D3He gas mixture. Standard hydrodynamic mixing cannot explain this observation, as hydrodynamic modeling including mix predicts a yield an order of magnitude lower than was observed. Instead, these results can be attributed to ion diffusive mix at the fuel-shell interface.

  12. QACINA

    Energy Science and Technology Software Center (OSTI)

    2003-09-19

    QACINA is an extensible multiphysics code that includes finite-element based solvers for 2D/3D time-dependent heat conduction, 2D time-dependent advection-diffusion, 2D time-dependent incompressible flow, 2D Lagrangian hydrodynamics. In addition, 2D/3D unstructured-grid finite-volume solvers are being developed for solving time-dependent advection-diffusion and the compressible Euler equations. QACINA uses a toolkit model for development and provides lightweight, high-performance and reusable code modules for agile development.

  13. Experimental Design of Hydrokinetic Resource Characterization

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    419 Field Measurements at River and Tidal Current Sites for Hydrokinetic Energy Development: Best Practices Manual September 2011 Prepared by Vincent S. Neary, Ph.D., P.E. 1 Budi Gunawan, Ph.D. 1 Marshall C. Richmond, Ph.D. P.E. 2 Vibhav Durgesh, Ph.D. 2 Brian Polagye, Ph.D. 3 Jim Thomson, Ph.D. 3 Marian Muste, Ph.D. 4 Arnie Fontaine, Ph.D. 5 1 Oak Ridge National Laboratory 2 Pacific Northwest National Laboratory 3 Northwest National Marine Renewable Energy Center, University of Washington 4

  14. Energy Literacy Social Studies Guides

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    much energy do I need? Energy Literacy Essential Principle 6: The amount of energy used by human society depends on many factors. C3 Framework for Social Studies Focus Indicators D1: Identify the disciplinary concepts and ideas associated with a supporting question that are open to interpretation. (D1.3.3-5) D2: Compare the benefits and costs of individual choices. (D2.Eco.1.3-5) D3: Use evidence to develop claims in response to compelling questions. (D3.4.3-5) D4: Present a summary of arguments

  15. Couplings between Chern-Simons gravities and 2p-branes

    SciTech Connect (OSTI)

    Miskovic, Olivera; Zanelli, Jorge

    2009-08-15

    The interaction between Chern-Simons (CS) theories and localized external sources (2p-branes) is analyzed. This interaction generalizes the minimal coupling between a point charge (0-brane) and a gauge connection. The external currents that define the 2p branes are covariantly constant (D-2p-1)-forms coupled to (2p-1) CS forms. The general expression for the sources--charged with respect to the corresponding gauge algebra--is presented, focusing on two special cases: 0-branes and (D-3)-branes. In any dimension, 0-branes are constructed as topological defects produced by a surface deficit of (D-2)-sphere in anti-de Sitter space, and they are not constant curvature spaces for D>3. They correspond to dimensionally continued black holes with negative mass. On the other hand, in the case of CS (super) gravities, the (D-3)-branes are naked conical singularities (topological defects) obtained by identification of points with a Killing vector. In 2+1 dimensions, extremal spinning branes of this type are Bogomol'nyi-Prasad-Sommerfield states. Stable (D-3)-branes are shown to exist also in higher dimensions, as well. Classical field equations are also discussed, and in the presence of sources there is a large number of inequivalent and disconnected sectors in solution space.

  16. ESnet Visualization Widgets

    Energy Science and Technology Software Center (OSTI)

    2012-07-01

    The ESnet Visualization widgets are various data visualization widgets for use in web browsers to aid in the visualization of computer networks. In particular the widgets are targetted at displaying timeseries and topology data. They were developed for use in the MyESnet portal but are general enough to be used other places. The widgets are built using the d3.js library.

  17. Science Headlines

    Office of Science (SC) Website

    -0500 12B69234-E106-461C-B008-1A4F342D3F6Dhttp:1.usa.gov20feQ3S A Respiratory Virus Provides Clues to Possible Treatments Purdue University researchers, working at the...

  18. News

    Office of Science (SC) Website

    glaciers. 12B69234-E106-461C-B008-1A4F342D3F6Dhttp:1.usa.gov20feQ3S A Respiratory Virus Provides Clues to Possible Treatments Purdue University researchers, working at the...

  19. Shell model description of band structure in 48Cr

    SciTech Connect (OSTI)

    Vargas, Carlos E.; Velazquez, Victor M.

    2007-02-12

    The band structure for normal and abnormal parity bands in 48Cr are described using the m-scheme shell model. In addition to full fp-shell, two particles in the 1d3/2 orbital are allowed in order to describe intruder states. The interaction includes fp-, sd- and mixed matrix elements.

  20. MEMORANDUfl J: FILE DATE

    Office of Legacy Management (LM)

    J: FILE DATE r so ---...w------m FROM: 9. 34oyc -w---...v----- SUBJECT: D3 Bo;s CL&;C J mL-;+J; - Rcc cap 049 'A :j: &336;s L-.fh w-f L-1 ALE"nirTE ---...

  1. PART I - THE SCHEDULE

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    D Contract No. DE-AC06-09RL14728 D-i PART I - THE SCHEDULE SECTION D PACKAGING AND MARKING TABLE OF CONTENTS D.1 PACKAGING ................................................................................................................ D-1 D.2 MARKING .................................................................................................................... D-1 D.3 SECURITY REQUIREMENTS ...................................................................................... D-1 Mission

  2. PART I - THE SCHEDULE

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    D Contract No. DE-AC27-08RV14800 D-i PART I - THE SCHEDULE SECTION D PACKAGING AND MARKING TABLE OF CONTENTS D.1 PACKAGING ................................................................................................................ D-1 D.2 MARKING .................................................................................................................... D-1 D.3 SECURITY REQUIREMENTS ...................................................................................... D-1 Tank

  3. 05_03_2002.tex

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    3 from (2002TI10): Energy levels of 5 Li, extended R-matrix prescription a E x J π ; T Γ cm b Γ p Γ d Γ p ∗ c Decay Reactions (MeV) (MeV) (MeV) (MeV) (MeV) (used in analysis) g.s. d 3 2 - ; 1 2 1.23 1.06 43.1 e 0.009 e p, α 3, 6, 9, 13, 18, 20, 23 1.49 1 2 - ; 1 2 6.60 3.78 16.4 e p, α 3, 9, 13, 18, 20 16.87 3 2 + ; 1 2 0.267 0.055 0.134 f γ, p, d, 3 He, α 3, 4, 5, 18, 20 19.28 3 2 - ; 1 2 0.959 0.001 0.040 g 0.741 n, p, d, 3 He, α 4, 5, 9 19.45 7 2 + ; 1 2 3.28 0.040 1.82 h p, d, 3

  4. CX-001422: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    A 3D-3C Reflection Seismic Survey and Data Integration to Identify the Seismic Response of Fractures and Permeable Zones Over a Known Geothermal Resource: Soda lake, Churchill County, NevadaCX(s) Applied: B3.1, A9Date: 03/29/2010Location(s): Soda Lake, NevadaOffice(s): Energy Efficiency and Renewable Energy, Golden Field Office

  5. SU-E-J-137: Image Registration Tool for Patient Setup in Korea Heavy Ion Medical Accelerator Center

    SciTech Connect (OSTI)

    Kim, M; Suh, T; Cho, W; Jung, W

    2015-06-15

    Purpose: A potential validation tool for compensating patient positioning error was developed using 2D/3D and 3D/3D image registration. Methods: For 2D/3D registration, digitally reconstructed radiography (DRR) and three-dimensional computed tomography (3D-CT) images were applied. The ray-casting algorithm is the most straightforward method for generating DRR. We adopted the traditional ray-casting method, which finds the intersections of a ray with all objects, voxels of the 3D-CT volume in the scene. The similarity between the extracted DRR and orthogonal image was measured by using a normalized mutual information method. Two orthogonal images were acquired from a Cyber-Knife system from the anterior-posterior (AP) and right lateral (RL) views. The 3D-CT and two orthogonal images of an anthropomorphic phantom and head and neck cancer patient were used in this study. For 3D/3D registration, planning CT and in-room CT image were applied. After registration, the translation and rotation factors were calculated to position a couch to be movable in six dimensions. Results: Registration accuracies and average errors of 2.12 mm ± 0.50 mm for transformations and 1.23° ± 0.40° for rotations were acquired by 2D/3D registration using an anthropomorphic Alderson-Rando phantom. In addition, registration accuracies and average errors of 0.90 mm ± 0.30 mm for transformations and 1.00° ± 0.2° for rotations were acquired using CT image sets. Conclusion: We demonstrated that this validation tool could compensate for patient positioning error. In addition, this research could be the fundamental step for compensating patient positioning error at the first Korea heavy-ion medical accelerator treatment center.

  6. Modeling the effects of vorinostat in vivo reveals both transient and delayed HIV transcriptional activation and minimal killing of latently infected cells

    SciTech Connect (OSTI)

    Ke, Ruian; Lewin, Sharon R.; Elliott, Julian H.; Perelson, Alan S.; Chakraborty, Arup K.

    2015-10-23

    Recent efforts to cure human immunodeficiency virus type-1 (HIV-1) infection have focused on developing latency reversing agents as a first step to eradicate the latent reservoir. The histone deacetylase inhibitor, vorinostat, has been shown to activate HIV RNA transcription in CD4+ T-cells and alter host cell gene transcription in HIV-infected individuals on antiretroviral therapy. In order to understand how latently infected cells respond dynamically to vorinostat treatment and determine the impact of vorinostat on reservoir size in vivo, we have constructed viral dynamic models of latency that incorporate vorinostat treatment. We fitted these models to data collected from a recent clinical trial in which vorinostat was administered daily for 14 days to HIV-infected individuals on suppressive ART. The results show that HIV transcription is increased transiently during the first few hours or days of treatment and that there is a delay before a sustained increase of HIV transcription, whose duration varies among study participants and may depend on the long term impact of vorinostat on host gene expression. Parameter estimation suggests that in latently infected cells, HIV transcription induced by vorinostat occurs at lower levels than in productively infected cells. Lastly, the estimated loss rate of transcriptionally induced cells remains close to baseline in most study participants, suggesting vorinostat treatment does not induce latently infected cell killing and thus reduce the latent reservoir in vivo.

  7. Primary Radiation Therapy for Head-and-Neck Cancer in the Setting of Human Immunodeficiency Virus

    SciTech Connect (OSTI)

    Klein, Emily A.; Guiou, Michael; Farwell, D. Gregory; Luu, Quang; Lau, Derick H.; Stuart, Kerri; Vaughan, Andrew; Vijayakumar, Srinivasan; Chen, Allen M.

    2011-01-01

    Purpose: To analyze outcomes after radiation therapy for head-and-neck cancer among a cohort of patients with human immunodeficiency virus (HIV). Methods and Materials: The medical records of 12 patients with serologic evidence of HIV who subsequently underwent radiation therapy to a median dose of 68 Gy (range, 64-72 Gy) for newly diagnosed squamous cell carcinoma of the head and neck were reviewed. Six patients (50%) received concurrent chemotherapy. Intensity-modulated radiotherapy was used in 6 cases (50%). All patients had a Karnofsky performance status of 80 or 90. Nine patients (75%) were receiving antiretroviral therapies at the time of treatment, and the median CD4 count was 460 (range, 266-800). Toxicity was graded according to the Radiation Therapy Oncology Group / European Organization for the Treatment of Cancer toxicity criteria. Results: The 3-year estimates of overall survival and local-regional control were 78% and 92%, respectively. Acute Grade 3+ toxicity occurred in 7 patients (58%), the most common being confluent mucositis (5 patients) and moist skin desquamation (4 patients). Two patients experienced greater than 10% weight loss, and none experienced more than 15% weight loss from baseline. Five patients (42%) experienced treatment breaks in excess of 10 cumulative days, although none required hospitalization. There were no treatment-related fatalities. Conclusions: Radiation therapy for head-and-neck cancer seems to be relatively well tolerated among appropriately selected patients with HIV. The observed rates of toxicity were comparable to historical controls without HIV.

  8. Chronic beryllium disease: Diagnosis and management

    SciTech Connect (OSTI)

    Rossman, M.D.

    1996-10-01

    Chronic beryllium disease is predominantly a pulmonary granulomatosis that was originally described in 1946. Symptoms usually include dyspnea and cough. Fever, anorexia, and weight loss are common. Skin lesions are the most common extrathoracic manifestation. Granulomatous hepatitis, hypercalcemia, and kidney stones can also occur. Radiographic and physiologic abnormalities are similar to those in sarcoidosis. While traditionally the pathologic changes included granulomas and cellular interstitial changes, the hallmark of the disease today is the well-formed granuloma. Immunologic studies have demonstrated a cell-mediated response to beryllium that is due to an accumulation of CD4{sup +} T cells at the site of disease activity. Diagnosis depends on the demonstration of pathologic changes (i.e., granuloma) and evidence that the granuloma was caused by a hypersensitivity to beryllium (i.e., positive lung proliferative response to beryllium). Using these criteria, the diagnosis of chronic beryllium disease can now be made before the onset of clinical symptoms. Whether, with early diagnosis, the natural course of this condition will be the same as when it was traditionally diagnosed is not known. Currently, corticosteroids are used to treat patients with significant symptoms or evidence of progressive disease. 21 refs.

  9. Identification of full-length transmitted/founder viruses and their progeny in primary HIV-1 infection

    SciTech Connect (OSTI)

    Korber, Bette; Hraber, Peter; Giorgi, Elena; Bhattacharya, T

    2009-01-01

    Identification of transmitted/founder virus genomes and their progeny by is a novel strategy for probing the molecular basis of HIV-1 transmission and for evaluating the genetic imprint of viral and host factors that act to constrain or facilitate virus replication. Here, we show in a cohort of twelve acutely infected subjects (9 clade B; 3 clade C), that complete genomic sequences of transmitted/founder viruses could be inferred using single genome amplification of plasma viral RNA, direct amplicon sequencing, and a model of random virus evolution. This allowed for the precise identification, chemical synthesis, molecular cloning, and biological analysis of those viruses actually responsible for productive clinical infection and for a comprehensive mapping of sequential viral genomes and proteomes for mutations that are necessary or incidental to the establishment of HIV-1 persistence. Transmitted/founder viruses were CD4 and CCR5 tropic, replicated preferentially in activated primary T-Iymphocytes but not monocyte-derived macrophages, and were effectively shielded from most heterologous or broadly neutralizing antibodies. By 3 months of infection, the evolving viral quasispecies in three subjects showed mutational fixation at only 2-5 discreet genomic loci. By 6-12 months, mutational fixation was evident at 18-27 genomic loci. Some, but not all, of these mutations were attributable to virus escape from cytotoxic Tlymphocytes or neutralizing antibodies, suggesting that other viral or host factors may influence early HIV -1 fitness.

  10. Transgenic Mouse Model of Chronic Beryllium Disease

    SciTech Connect (OSTI)

    Gordon, Terry

    2009-05-26

    Animal models provide powerful tools for dissecting dose-response relationships and pathogenic mechanisms and for testing new treatment paradigms. Mechanistic research on beryllium exposure-disease relationships is severely limited by a general inability to develop a sufficient chronic beryllium disease animal model. Discovery of the Human Leukocyte Antigen (HLA) - DPB1Glu69 genetic susceptibility component of chronic beryllium disease permitted the addition of this human beryllium antigen presentation molecule to an animal genome which may permit development of a better animal model for chronic beryllium disease. Using FVB/N inbred mice, Drs. Rubin and Zhu, successfully produced three strains of HLA-DPB1 Glu 69 transgenic mice. Each mouse strain contains a haplotype of the HLA-DPB1 Glu 69 gene that confers a different magnitude of odds ratio (OR) of risk for chronic beryllium disease: HLA-DPB1*0401 (OR = 0.2), HLA-DPB1*0201 (OR = 15), HLA-DPB1*1701 (OR = 240). In addition, Drs. Rubin and Zhu developed transgenic mice with the human CD4 gene to permit better transmission of signals between T cells and antigen presenting cells. This project has maintained the colonies of these transgenic mice and tested the functionality of the human transgenes.

  11. Modeling the effects of vorinostat in vivo reveals both transient and delayed HIV transcriptional activation and minimal killing of latently infected cells

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Ke, Ruian; Lewin, Sharon R.; Elliott, Julian H.; Perelson, Alan S.; Chakraborty, Arup K.

    2015-10-23

    Recent efforts to cure human immunodeficiency virus type-1 (HIV-1) infection have focused on developing latency reversing agents as a first step to eradicate the latent reservoir. The histone deacetylase inhibitor, vorinostat, has been shown to activate HIV RNA transcription in CD4+ T-cells and alter host cell gene transcription in HIV-infected individuals on antiretroviral therapy. In order to understand how latently infected cells respond dynamically to vorinostat treatment and determine the impact of vorinostat on reservoir size in vivo, we have constructed viral dynamic models of latency that incorporate vorinostat treatment. We fitted these models to data collected from a recentmore » clinical trial in which vorinostat was administered daily for 14 days to HIV-infected individuals on suppressive ART. The results show that HIV transcription is increased transiently during the first few hours or days of treatment and that there is a delay before a sustained increase of HIV transcription, whose duration varies among study participants and may depend on the long term impact of vorinostat on host gene expression. Parameter estimation suggests that in latently infected cells, HIV transcription induced by vorinostat occurs at lower levels than in productively infected cells. Lastly, the estimated loss rate of transcriptionally induced cells remains close to baseline in most study participants, suggesting vorinostat treatment does not induce latently infected cell killing and thus reduce the latent reservoir in vivo.« less

  12. Synergistic activity profile of griffithsin in combination with tenofovir, maraviroc and enfuvirtide against HIV-1 clade C

    SciTech Connect (OSTI)

    Ferir, Geoffrey; Palmer, Kenneth E.; Schols, Dominique

    2011-09-01

    Griffithsin (GRFT) is possibly the most potent anti-HIV peptide found in natural sources. Due to its potent and broad-spectrum antiviral activity and unique safety profile it has great potential as topical microbicide component. Here, we evaluated various combinations of GRFT against HIV-1 clade B and clade C isolates in primary peripheral blood mononuclear cells (PBMCs) and in CD4{sup +} MT-4 cells. In all combinations tested, GRFT showed synergistic activity profile with tenofovir, maraviroc and enfuvirtide based on the median effect principle with combination indices (CI) varying between 0.34 and 0.79 at the calculated EC{sub 95} level. Furthermore, the different glycosylation patterns on the viral envelope of clade B and clade C gp120 had no observable effect on the synergistic interactions. Overall, we can conclude that the evaluated two-drug combination increases their antiviral potency and supports further clinical investigations in pre-exposure prophylaxis for GRFT combinations in the context of HIV-1 clade C infection.

  13. Biomarkers of Radiosensitivity in A-Bomb Survivors Pregnant at the Time of Bombings in Hiroshima and Nagasaki

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Miles, Edward F.; Tatsukawa, Yoshimi; Funamoto, Sachiyo; Kamada, Naoko; Nakashima, Eiji; Kodama, Yoshiaki; Seed, Thomas; Kusonoki, Yoichiro; Nakachi, Kei; Fujiwara, Saeko; et al

    2011-01-01

    Purpose . There is evidence in the literature of increased maternal radiosensitivity during pregnancy. Materials and Methods . We tested this hypothesis using information from the atomic-bomb survivor cohort, that is, the Adult Health Study database at the Radiation Effects Research Foundation, which contains data from a cohort of women who were pregnant at the time of the bombings of Hiroshima and Nagasaki. Previous evaluation has demonstrated long-term radiation dose-response effects. Results/Conclusions . Data on approximately 250 women were available to assess dose-response rates for serum cholesterol, white blood cell count, erythrocyte sedimentation rate, and serum hemoglobin, and onmore » approximately 85 women for stable chromosome aberrations, glycophorin A locus mutations, and naïve CD4 T-cell counts. Although there is no statistically significant evidence of increased radiosensitivity in pregnant women, the increased slope of the linear trend line in the third trimester with respect to stable chromosome aberrations is suggestive of an increased radiosensitivity.« less

  14. Combined Treatment Effects of Radiation and Immunotherapy: Studies in an Autochthonous Prostate Cancer Model

    SciTech Connect (OSTI)

    Wada, Satoshi; Harris, Timothy J.; Tryggestad, Erik; Yoshimura, Kiyoshi; Zeng, Jing; Yen, Hung-Rong; Getnet, Derese; Grosso, Joseph F.; Bruno, Tullia C.; De Marzo, Angelo M.; and others

    2013-11-15

    Purpose: To optimize the combination of ionizing radiation and cellular immunotherapy using a preclinical autochthonous model of prostate cancer. Methods and Materials: Transgenic mice expressing a model antigen under a prostate-specific promoter were treated using a platform that integrates cone-beam CT imaging with 3-dimensional conformal therapy. Using this technology we investigated the immunologic and therapeutic effects of combining ionizing radiation with granulocyte/macrophage colony-stimulating factor-secreting cellular immunotherapy for prostate cancer in mice bearing autochthonous prostate tumors. Results: The combination of ionizing radiation and immunotherapy resulted in a significant decrease in pathologic tumor grade and gross tumor bulk that was not evident with either single-modality therapy. Furthermore, combinatorial therapy resulted in improved overall survival in a preventive metastasis model and in the setting of established micrometastases. Mechanistically, combined therapy resulted in an increase of the ratio of effector-to-regulatory T cells for both CD4 and CD8 tumor-infiltrating lymphocytes. Conclusions: Our preclinical model establishes a potential role for the use of combined radiation-immunotherapy in locally advanced prostate cancer, which warrants further exploration in a clinical setting.

  15. Triviality of the BCS-BEC crossover in extended dimensions: Implications for the ground state energy

    SciTech Connect (OSTI)

    Nussinov, Zohar [Department of Physics, Washington University, St. Louis, Missouri 63160-4899 (United States); Nussinov, Shmuel [School of Physics and Astronomy, Tel Aviv University, Ramat-Aviv, Tel Aviv 69978 (Israel)

    2006-11-15

    Cold atom traps and certain neutron star layers may contain fermions with separation much larger than the range of pairwise potentials yet much shorter than the scattering length. Such systems can display universal characteristics independent of the details of the short range interactions. Our main result is that for space dimensions D smaller than two and larger than four a specific extension of this problem is amenable to exact results. In particular, the energy per particle at the BCS-BEC crossover point is equal to the energy of the free fermion system in all D{<=}2 whereas this energy is rigorously nonpositive (and potentially vanishing) in all D{>=}4. We discuss the D=3 case. A particular unjustified recipe suggests {xi}=1/2 in D=3.

  16. Effects of polychlorinated biphenyls and nutritional restriction on barbituate-induced sleeping times and selected blood characteristics in raccoons (Procyon lotor)

    SciTech Connect (OSTI)

    Montz, W.E.; Card, W.C.; Kirkpatrick, R.L.

    1982-05-01

    Hepatic microsomal enzyme activity was induced in wild-trapped raccoons (Procyon lotor) and selected blood characteristics were measured in an effort to detect responses due to PCB ingestion, nutritional restriction, and their interactions. Barbiturate-induced sleeping times were used as an index of hepatic microsomal activity because they have been used reliably by other workers. Blood characteristics examined in the study were nonesterified fatty acids (NEFA), cholesterol, and three ketone bodies (D-(-)-3-hydroxybutyrate, acetoacetate, and acetone). Results show a reduction in sleeping times, elevated NEFA and D-(-)-3-hydroxybutyrate concentrations, and lower cholesterol concentrations in PCB-treated groups. A highly significant interaction between PCB treatment and nutritional restriction was observed in acetoacetate concentrations. (JMT)

  17. Better Buildings Network View

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ...RP&3;WZR&3;FDOOV&3;HDFK&3;VHFRQG&3;DQG&3;IRXUWK 7KXUVGDV&3;RI&3;WKH&3;PRQWK&3;WR&3;RQH&3;FDOO&3;SHU&3;ZHHN&3;IRXU&3;WLPHV... KDYH&3;EHHQ&3;VHUYHG&3;LQ&3;WKH&3;SDVW&3;ILYH&3;HDUV&17;&3; V&3;D&3;IRUPHU&3;%HWWHU&3;%XLOGLQJV&3;1HLJKERUKRRG&3;3URJU...

  18. The Orosomucoid 1 protein is involved in the vitamin D – mediated macrophage de-activation process

    SciTech Connect (OSTI)

    Gemelli, Claudia; Martello, Andrea; Montanari, Monica; Zanocco Marani, Tommaso; Salsi, Valentina; Zappavigna, Vincenzo; Parenti, Sandra; Vignudelli, Tatiana; Selmi, Tommaso; Ferrari, Sergio; Grande, Alexis

    2013-12-10

    Orosomucoid 1 (ORM1), also named Alpha 1 acid glycoprotein A (AGP-A), is an abundant plasma protein characterized by anti-inflammatory and immune-modulating properties. The present study was designed to identify a possible correlation between ORM1 and Vitamin D3 (1,25(OH)2D3), a hormone exerting a widespread effect on cell proliferation, differentiation and regulation of the immune system. In particular, the data described here indicated that ORM1 is a 1,25(OH)2D3 primary response gene, characterized by the presence of a VDRE element inside the 1 kb sequence of its proximal promoter region. This finding was demonstrated with gene expression studies, Chromatin Immunoprecipitation and luciferase transactivation experiments and confirmed by VDR full length and dominant negative over-expression. In addition, several experiments carried out in human normal monocytes demonstrated that the 1,25(OH)2D3 – VDR – ORM1 pathway plays a functional role inside the macrophage de-activation process and that ORM1 may be considered as a signaling molecule involved in the maintenance of tissue homeostasis and remodeling. - Highlights: • ORM1 is a Vitamin D primary response gene. • VD and its receptor VDR are involved in the de-activation process mediated by human resident macrophages. • The signaling pathway VD-VDR-ORM1 plays an important role in the control of macrophage de-activation process. • ORM1 may be defined as a signaling molecule implicated in the maintenance of tissue homeostasis and remodeling.

  19. DOE Hydropower Program Annual Report for FY 2000

    SciTech Connect (OSTI)

    Sale, M. J.; Cada, G. F.; Dauble, D. D.; Rinehart, B. N.; Sommers, G. L.; Flynn, J. V.; Brookshier, P. A.

    2001-04-17

    This report describes the activities of the U.S. Department of Energy (DOE) Hydropower Program during Fiscal Year 2000 (October 1, 1999, to September 30, 2000). Background, current activities, and future plans are presented in the following sections for all components of the Program. Program focus for FY 2000 was on (1) advanced turbine development, (2) basic and applied R&D, (3) environmental mitigation, (4) low head/low power hydropower technology, and (5) technology transfer.

  20. Higher derivative couplings in theories with sixteen supersymmetries

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Lin, Ying -Hsuan; Shao, Shu -Heng; Yin, Xi; Wang, Yifan

    2015-12-15

    We give simple arguments for new non-renormalization theorems on higher derivative couplings of gauge theories to supergravity, with sixteen supersymmetries, by considerations of brane-bulk superamplitudes. This leads to some exact results on the effective coupling of D3-branes in type IIB string theory. As a result, we also derive exact results on higher dimensional operators in the torus compactification of the six dimensional (0, 2) superconformal theory.

  1. NMMSS News jan 2013.cdr

    National Nuclear Security Administration (NNSA)

    Crosswalk to SAMS D-2/D-3 Updates Training Courses NMMSS Germantown Operations NMMSS Team Members NMMSS Due Dates 2013 NMMSS Calendar NMMSS Mailing Addresses January 2013 NMMSS 2012 Meeting Presentations and Photographs A new link has been added to the NMMSS homepage "NMMSS 2012 Meeting Presentations and Photographs." From here you may open and down load the presentations from the 2012 users training meeting as well as photographs. Homepage link:

  2. NMMSS Reports | National Nuclear Security Administration | (NNSA)

    National Nuclear Security Administration (NNSA)

    NMMSS Reports U.S. Department of Energy / U.S. Nuclear Regulatory Commission Nuclear Materials Management & Safeguards System NMMSS Reports DOE D-23 DOE Contractors Rev August 2016 DOE Manual 474.2 NMMSS User Guide - Coming Soon! NRC D-24 NRC Licensees Rev August 2016 NUREG/BR-0007, Rev. 6 NUREG/BR-0006, Rev. 7 The electronic version of the D-2, D-3, and D-15 RIS directories are available upon request.

  3. Microsoft PowerPoint - 1-Mike Grauwelman's presentation - 5.20.14_comp

    Office of Legacy Management (LM)

    REDEVELOPMENT USDOE MOUND FACILITY MIAMISBURG, OHIO MIKE GRAUWELMAN MOUND DEVELOPMENT CORPORATION (RETIRED) AGENDA * Mound Background * DOE History * Community Organizational Structure * Redevelopment Plan * Economic Development 2 MOUND SITE Site Information: * Founded 1948 * Manhattan Project * 306 Acres * 1.3M Sq. Ft. Buildings * Topo Variance 180' * Buried Valley Aquifer Workforce Information: * Peak Employment: 2,400 * 25% PhD's 3 MOUND MISSIONS Recent Missions * Environmental Remediation

  4. June2010KeyEISChart.pdf | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    June2010KeyEISChart.pdf June2010KeyEISChart.pdf June2010KeyEISChart.pdf (59.25 KB) More Documents & Publications Schedules of Key Environmental Impact Statements Demonstration of a Variable Phase Turbine Power System for Low Temperature Geothermal Resources A 3D-3C Reflection Seismic Survey and Data Integration to Identify the Seismic Response of Fractures and Permeable Zones Over a Known Geothermal Resource at Soda Lake, Churchhill Co., NV

  5. Processes for producing polyhydroxybutyrate and related polyhydroxyalkanoates in the plastids of higher plants

    DOE Patents [OSTI]

    Somerville, C.R.; Nawrath, C.; Poirier, Y.

    1997-03-11

    The present invention relates to a process for producing poly-D-(-)-3-hydroxybutyric acid (PHB) and related polyhydroxyalkanoates (PHA) in the plastids of plants. The production of PHB is accomplished by genetically transforming plants with modified genes from microorganisms. The genes encode the enzymes required to synthesize PHB from acetyl-CoA or related metabolites and are fused with additional plant sequences for targeting the enzymes to the plastid. 37 figs.

  6. Processes for producing polyhydroxybutyrate and related polyhydroxyalkanoates in the plastids of higher plants

    DOE Patents [OSTI]

    Somerville, Christopher R.; Nawrath, Christiane; Poirier, Yves

    1997-03-11

    The present invention relates to a process for producing poly-D-(-)-3-hydroxybutyric acid (PHB) and related polyhydroxyalkanoates (PHA) in the plastids of plants. The production of PHB is accomplished by genetically transforming plants with modified genes from microorganisms. The genes encode the enzymes required to synthesize PHB from acetyl-CoA or related metabolites and are fused with additional plant sequences for targeting the enzymes to the plastid.

  7. Method to Reduce Neutron Production in Small Clean Fusion Reactors Inventor

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    --- Samuel A. Cohen | Princeton Plasma Physics Lab Method to Reduce Neutron Production in Small Clean Fusion Reactors Inventor --- Samuel A. Cohen This invention describes a method to reduce neutron production of D-3He-fueled, steady state, small FRC fusion reactors using periodic, co-streaming, energetic ion beams generated by RF. Use of this method will lessen damage to and activation of reactor components and, in doing so, can advance the development of fusion reactors for electrical

  8. Microsoft PowerPoint - 05_ARM 2007 Meeting.ppt [Compatibility Mode]

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Multi-Scale Aerosol/Cloud Interactions From ARM Measurements to Global Modeling Catherine Chuang, Steve Chin, Shaocheng Xie, James Kelly, and James Boyle Lawrence Livermore National Laboratory, Livermore Objectives Examine the influence of aerosol compositions on droplet formation and review different cloud nucleation parameterizations.- Micro Scale Investigate impacts of aerosols and autoconversion schemes on cloud properties over SGP with 2-D/3-D cloud resolving models. - Cloud Scale Explore

  9. Microsoft Word - NT0005682R21

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    10.8 374 1080 D3 0.1 5.9 0.3667 0.0209 6.3 8440 7.34 -212 11.1 312 900 D4 <0.03 3.9 0.2189 0.0186 6.2 8420 7.18 212 11.3 280 1020 nd- not determined Cd bdl (0.000025 mgL), Se...

  10. Measurement of the 3s1/2-3p3/2 resonance line of sodiumlike Eu52+

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Trbert, E.; Beiersdorfer, P.; Hell, N.; Brown, G. V.

    2015-08-20

    We have measured the 3s1/2-3p3/2 transition in sodiumlike Eu52+ situated at 41.232 with an uncertainty of 73 ppm. Our measurement extends previous high-precision measurements into the 561/2-3p3/2 and 3p1/2-3d3/2 transitions in the neighboring magnesiumlike, aluminumlike, and siliconlike europium ions.

  11. EFG IO?-90)

    Office of Legacy Management (LM)

    .?GE = 1325 d +3-891 EFG IO?-90) ' United States Government *memorandum DATE: FEB 6 1991 . . I ;' 1 , q' *_* Departrnkt of Energy --. -* r- i .,. 1 .' ;I "--' I ,_ % I : [ -- ,: -.. ! j i 1: \ REPLY TO ATTN OF: EM-421 SUBJECT: Authority Determination-- Former Beryllium Production Facility in Luckey, ' Ohio TO: The File The attached review documents the basis for determining whether DOE has authority for taking remedial action at the former beryllium production facility in Luckey, Ohio,

  12. Microsoft PowerPoint - DongHuang_ARMposter2009.ppt [Compatibility Mode]

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    cloud water distribution from an air-borne scanning microwave radiometer: Wakasa Bay field experiment results Dong Huang, Albin Gasiewski, and Warren Wiscombe 1. A mobile cloud tomography setup has some advantages over a fixed one. The tomographic method involves scanning clouds from a multiplicity of directions and locations, and inverting the resulting radiometric measurements to obtain 2D/3D cloud water distributions. A fixed ground-based setup will needed more than four microwave radiometers

  13. CX-004109: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Phase 2 - A 3D-3C Reflection Seismic Survey and Data Integration to Identify the Seismic Response of Fractures and Permeable Zones over a Known Geothermal Resource: Soda Lake, Churchill County, NevadaCX(s) Applied: B3.1, B3.7Date: 09/29/2010Location(s): Soda Lake, NevadaOffice(s): Energy Efficiency and Renewable Energy, Golden Field Office

  14. CX-006567: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Recovery Act: A 3D-3C Reflection Seismic Survey and Data Integration to Identify the Seismic Response of Fractures and Permeable Zones Over a Known Geothermal Resource: Soda Lake, Churchill County, NevadaCX(s) Applied: A9, B3.1, B3.7Date: 08/24/2011Location(s): Churchill County, NevadaOffice(s): Energy Efficiency and Renewable Energy, Golden Field Office

  15. Instrumentation upgrades for the Macromolecular Crystallography beamlines

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of the Swiss Light Source | Stanford Synchrotron Radiation Lightsource Instrumentation upgrades for the Macromolecular Crystallography beamlines of the Swiss Light Source Monday, October 29, 2012 - 2:00am SSRL, Bldg. 137, Rm. 322 Martin Fuchs, MX Group, Swiss Light Source; Paul Scherrer Institute (Villigen, Switzerland) A new unified diffractometer - the D3 - has been developed for the three MX beamlines. The first of the instruments is in general user operation at beamline X10SA since April

  16. Dual Panal Planar Portal

    Energy Science and Technology Software Center (OSTI)

    2000-12-01

    The D3P system is designed to provide a very fast imaging system in order to search for weapons on persons in an airport environment. The complete vision of the D3P system is to have two array systems facing each other. Version 2.3 of the software is designed to control and process data from a single panel. A second panel is expected to be added at a future date and the software will be modified atmore » that time to integrate the images from two panels at one time. The D3P software can be segmented into three specific tasks. The first task is data acquisition and scanner control. At the operator's request, this task commands the scanner to move and the radar transceiver-array to send data to the computer system in a known and well-ordered manner. The array is moved over the complete aperture in 1 to 2 seconds. At the completion of the array movement the second software task reconstructs the high-resolution image from the radar data utilizing the integrated DSP board. The third task displays the result to the computer screen for user review and analysis.« less

  17. Benchmarking density functional theory predictions of framework structures and properties in a chemically diverse test set of metal-organic frameworks

    SciTech Connect (OSTI)

    Nazarian, Dalar; Ganesh, P.; Sholl, David S.

    2015-09-30

    We compiled a test set of chemically and topologically diverse Metal–Organic Frameworks (MOFs) with high accuracy experimentally derived crystallographic structure data. The test set was used to benchmark the performance of Density Functional Theory (DFT) functionals (M06L, PBE, PW91, PBE-D2, PBE-D3, and vdW-DF2) for predicting lattice parameters, unit cell volume, bonded parameters and pore descriptors. On average PBE-D2, PBE-D3, and vdW-DF2 predict more accurate structures, but all functionals predicted pore diameters within 0.5 Å of the experimental diameter for every MOF in the test set. The test set was also used to assess the variance in performance of DFT functionals for elastic properties and atomic partial charges. The DFT predicted elastic properties such as minimum shear modulus and Young's modulus can differ by an average of 3 and 9 GPa for rigid MOFs such as those in the test set. Moreover, we calculated the partial charges by vdW-DF2 deviate the most from other functionals while there is no significant difference between the partial charges calculated by M06L, PBE, PW91, PBE-D2 and PBE-D3 for the MOFs in the test set. We find that while there are differences in the magnitude of the properties predicted by the various functionals, these discrepancies are small compared to the accuracy necessary for most practical applications.

  18. CAHE Technology Help Desk

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Dr. Russell Hardy, Director CEMRC Ambient Air Sampler Locations New Mexico State University New Mexico State University Sampling Site Sample Period Am241 Bq/Sample Pu239+240 Bq/Sample Pu238 Bq/Sample On Site #106 109 Yards NW of WIPP Exh. Shaft 2/11/14 - 2/18/14 1.300 0.115 0.004 2/18/14 - 2/25/14 0.020 0.004 0.004 2/25/14 - 3/4/14 0.007 3/4/14 - 3/11/14 n/d n/d n/d 3/11/14 - 3/21/14 0.013 n/d n/d 3/21/14 - 3/29/14 0.0005 n/d n/d 3/29/14 - 4/4/14 0.0005 n/d n/d Near Field #107 0.6 Miles NW of

  19. Regulatory T Cells Promote β-Catenin–Mediated Epithelium-to-Mesenchyme Transition During Radiation-Induced Pulmonary Fibrosis

    SciTech Connect (OSTI)

    Xiong, Shanshan; Pan, Xiujie; Xu, Long; Yang, Zhihua; Guo, Renfeng; Gu, Yongqing; Li, Ruoxi; Wang, Qianjun; Xiao, Fengjun; Du, Li; Zhou, Pingkun; Zhu, Maoxiang

    2015-10-01

    Purpose: Radiation-induced pulmonary fibrosis results from thoracic radiation therapy and severely limits radiation therapy approaches. CD4{sup +}CD25{sup +}FoxP3{sup +} regulatory T cells (Tregs) as well as epithelium-to-mesenchyme transition (EMT) cells are involved in pulmonary fibrosis induced by multiple factors. However, the mechanisms of Tregs and EMT cells in irradiation-induced pulmonary fibrosis remain unclear. In the present study, we investigated the influence of Tregs on EMT in radiation-induced pulmonary fibrosis. Methods and Materials: Mice thoraxes were irradiated (20 Gy), and Tregs were depleted by intraperitoneal injection of a monoclonal anti-CD25 antibody 2 hours after irradiation and every 7 days thereafter. Mice were treated on days 3, 7, and 14 and 1, 3, and 6 months post irradiation. The effectiveness of Treg depletion was assayed via flow cytometry. EMT and β-catenin in lung tissues were detected by immunohistochemistry. Tregs isolated from murine spleens were cultured with mouse lung epithelial (MLE) 12 cells, and short interfering RNA (siRNA) knockdown of β-catenin in MLE 12 cells was used to explore the effects of Tregs on EMT and β-catenin via flow cytometry and Western blotting. Results: Anti-CD25 antibody treatment depleted Tregs efficiently, attenuated the process of radiation-induced pulmonary fibrosis, hindered EMT, and reduced β-catenin accumulation in lung epithelial cells in vivo. The coculture of Tregs with irradiated MLE 12 cells showed that Tregs could promote EMT in MLE 12 cells and that the effect of Tregs on EMT was partially abrogated by β-catenin knockdown in vitro. Conclusions: Tregs can promote EMT in accelerating radiation-induced pulmonary fibrosis. This process is partially mediated through β-catenin. Our study suggests a new mechanism for EMT, promoted by Tregs, that accelerates radiation-induced pulmonary fibrosis.

  20. ZFAT plays critical roles in peripheral T cell homeostasis and its T cell receptor-mediated response

    SciTech Connect (OSTI)

    Doi, Keiko; Central Research Institute for Advanced Molecular Medicine, Fukuoka University, Fukuoka; Central Research Institute of Life Sciences for the Next Generation of Women Scientists, Fukuoka University, Fukuoka ; Fujimoto, Takahiro; Central Research Institute for Advanced Molecular Medicine, Fukuoka University, Fukuoka ; Okamura, Tadashi; Ogawa, Masahiro; Tanaka, Yoko; Mototani, Yasumasa; Goto, Motohito; Ota, Takeharu; Matsuzaki, Hiroshi; Kuroki, Masahide; Tsunoda, Toshiyuki; Central Research Institute for Advanced Molecular Medicine, Fukuoka University, Fukuoka ; Sasazuki, Takehiko; Shirasawa, Senji; Central Research Institute for Advanced Molecular Medicine, Fukuoka University, Fukuoka

    2012-08-17

    Highlights: Black-Right-Pointing-Pointer We generated Cd4-Cre-mediated T cell-specific Zfat-deficient mice. Black-Right-Pointing-Pointer Zfat-deficiency leads to reduction in the number of the peripheral T cells. Black-Right-Pointing-Pointer Impaired T cell receptor-mediated response in Zfat-deficient peripheral T cells. Black-Right-Pointing-Pointer Decreased expression of IL-7R{alpha}, IL-2R{alpha} and IL-2 in Zfat-deficient peripheral T cells. Black-Right-Pointing-Pointer Zfat plays critical roles in peripheral T cell homeostasis. -- Abstract: ZFAT, originally identified as a candidate susceptibility gene for autoimmune thyroid disease, has been reported to be involved in apoptosis, development and primitive hematopoiesis. Zfat is highly expressed in T- and B-cells in the lymphoid tissues, however, its physiological function in the immune system remains totally unknown. Here, we generated the T cell-specific Zfat-deficient mice and demonstrated that Zfat-deficiency leads to a remarkable reduction in the number of the peripheral T cells. Intriguingly, a reduced expression of IL-7R{alpha} and the impaired responsiveness to IL-7 for the survival were observed in the Zfat-deficient T cells. Furthermore, a severe defect in proliferation and increased apoptosis in the Zfat-deficient T cells following T cell receptor (TCR) stimulation was observed with a reduced IL-2R{alpha} expression as well as a reduced IL-2 production. Thus, our findings reveal that Zfat is a critical regulator in peripheral T cell homeostasis and its TCR-mediated response.

  1. Inhibition of human peripheral blood lymphocyte function by protoporphyrin and longwave ultraviolet light

    SciTech Connect (OSTI)

    Barrett, K.E.; Yen, A.; Montisano, D.; Gigli, I.; Bigby, T.D.

    1994-10-01

    Modulation of immunologic effector cells by exogenous photoactive substances has been advanced as an underlying mechanism for the efficacy of various photochemotherapeutic regimens. It is also possible that endogenous photosensitizers, such as protoporphyrin, could similarly modify the function of immune cell types. The authors examined the effects of protoporphyrin plus longwave UV light on the ability of human PBL to proliferate in response to mitogens. Noncytotoxic dosages of protoporphyrin plus UV light suppressed PHA-stimulated proliferation of both PBMC and enriched T cells. CD8{sup +} cells were more sensitive to this inhibitory effect than CD4{sup +} cells. The inhibitory effect was also observed when proliferation was induced by the combination of a phorbol ester and ionomycin. Inhibition of PBMC proliferation was associated with inhibition of IL-2 secretion but proliferation was not restored with exogenous IL-2. Instead, the effect of protoporphyrin plus UV light may be on IL-2R. Cells treated with protoporphyrin and UV light did not display the increase in CD25 and {beta}-chain of the IL-2R induced by PHA in control cells. In contrast to the effects of protoporphyrin and UV light on IL-2 and IL-2R {alpha}-chain protein expression, the accumulation of mRNA for these proteins induced by PHA was unaffected. None of the effects of protoporphyrin plus UV light on lymphocytes were observed in control experiments where cells were treated with either protoporphyrin or UV light alone. They conclude that biologically relevant dosages of protoporphyrin and UV light modify the function of circulating lymphocytes. 26 refs., 8 figs., 1 tab.

  2. Generation of knock-in primary human T cells using Cas9 ribonucleoproteins

    SciTech Connect (OSTI)

    Schumann, Kathrin; Lin, Steven; Boyer, Eric; Simeonov, Dimitre R.; Subramaniam, Meena; Gate, Rachel E.; Haliburton, Genevieve E.; Ye, Chun J.; Bluestone, Jeffrey A.; Doudna, Jennifer A.; Marson, Alexander

    2015-07-27

    T-cell genome engineering holds great promise for cell-based therapies for cancer, HIV, primary immune deficiencies, and autoimmune diseases, but genetic manipulation of human T cells has been challenging. Improved tools are needed to efficiently “knock out” genes and “knock in” targeted genome modifications to modulate T-cell function and correct disease-associated mutations. CRISPR/Cas9 technology is facilitating genome engineering in many cell types, but in human T cells its efficiency has been limited and it has not yet proven useful for targeted nucleotide replacements. Here we report efficient genome engineering in human CD4+ T cells using Cas9:single-guide RNA ribonucleoproteins (Cas9 RNPs). Cas9 RNPs allowed ablation of CXCR4, a coreceptor for HIV entry. Cas9 RNP electroporation caused up to ~40% of cells to lose high-level cell-surface expression of CXCR4, and edited cells could be enriched by sorting based on low CXCR4 expression. Importantly, Cas9 RNPs paired with homology-directed repair template oligonucleotides generated a high frequency of targeted genome modifications in primary T cells. Targeted nucleotide replacement was achieved in CXCR4 and PD-1 (PDCD1), a regulator of T-cell exhaustion that is a validated target for tumor immunotherapy. Deep sequencing of a target site confirmed that Cas9 RNPs generated knock-in genome modifications with up to ~20% efficiency, which accounted for up to approximately one-third of total editing events. These results establish Cas9 RNP technology for diverse experimental and therapeutic genome engineering applications in primary human T cells.

  3. Molecular basis of the structural stability of a Top7-based scaffold at extreme pH and temperature conditions

    SciTech Connect (OSTI)

    Soares, Thereza A.; Boschek, Curt B.; Apiyo, David O.; Baird, Cheryl L.; Straatsma, TP

    2010-07-01

    The development of stable scaffolds that can tolerate environmental extremes has an immense potential for applications in industry and defense. Recently, we have engineered an eight-residue loop into the de novo designed Top7 protein, which specifically binds the glycoprotein CD4. The robust properties of the Top7, coupled with the ease in production, make it a robust scaffold to design novel functionalities for use under extreme environmental conditions. In the present work, a series of explicit-solvent molecular dynamics simulations are reported which investigates the effect of mutations and extreme conditions of temperature and pH on the structure, stability, and dynamics of the native and engineered Top7. These simulations indicate that i. The structural dynamics of the engineered and native Top7 in solution are equivalent under corresponding conditions of pH and temperature. Ensemble-averaged structures of the native and engineered Top7 maintain the overall tertiary structure pattern, albeit with loss of helical content when at low pH and high-temperature conditions. Mutations of residues E43A, D46A, E67A, E69A, EA81A along the ?-helices of the engineered Top7 did not lead to significant changes in the native fold under pH 2 and 400 K, suggesting that the helices can accommodate varying sequences. iii. The anti-parallel ?-sheet is the structural core responsible for the stability of the native and engineered Top7 and is well maintained under extreme pH and temperature conditions. These findings indicate that the insertion of an eight-residue loop into the structure of Top7 does not adversely affect the global fold or the structural stability of the Top7 scaffold.

  4. The effects of deoxynivalenol on gene expression in the murine thymus

    SciTech Connect (OSTI)

    Kol, Sandra W.M. van; Hendriksen, Peter J.M.; Loveren, Henk van; Peijnenburg, Ad

    2011-02-01

    Deoxynivalenol (DON) is a mycotoxin produced by several Fusarium species and is often detected in grains. Because of its high abundance, there has been a large interest in the effects of DON in animals and humans. DON is known to be immunosuppressive at high concentrations and immunostimulatory at low concentrations. The present study aimed to acquire insight into the modes of action of DON. For this, C57Bl6 mice were orally exposed to 5, 10, or 25 mg/kg bw DON for 3, 6, or 24 h and thymuses were subjected to genome-wide expression microarray analysis. Gene set enrichment analysis (GSEA) demonstrated that DON downregulated genes involved in proliferation, mitochondria, protein synthesis, and ribosomal proteins. Furthermore, GSEA showed a selective downregulation of genes highly expressed at the early precursor thymocytes stage. This indicates that early precursor thymocytes, particularly at the double-positive CD4+CD8+ stage, are more vulnerable to DON than very early or late precursor thymocytes. There was a large overlap of genes upregulated by DON with genes previously reported to be either upregulated during T cell activation or upregulated during negative selection of thymocytes that recognize 'self-antigens'. This indicates that DON induces cellular events that also occur after activation of the T cell receptor, for example, release of calcium from the endoplasmatic reticulum. This T cell activation in the thymus then evokes negative selection and depletion of thymocytes, which provides a plausible explanation for the high sensitivity of the thymus for DON exposure. The expression patterns of four genes indicative for some of the processes that were affected after DON treatment were confirmed using real-time PCR. Immunocytological experiments with primary mouse thymocytes demonstrated the translocation of NFAT from the cytoplasm into the nucleus upon exposure top DON, thus providing further evidence for the involvement of T cell activation.

  5. Generation of knock-in primary human T cells using Cas9 ribonucleoproteins

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Schumann, Kathrin; Lin, Steven; Boyer, Eric; Simeonov, Dimitre R.; Subramaniam, Meena; Gate, Rachel E.; Haliburton, Genevieve E.; Ye, Chun J.; Bluestone, Jeffrey A.; Doudna, Jennifer A.; et al

    2015-07-27

    T-cell genome engineering holds great promise for cell-based therapies for cancer, HIV, primary immune deficiencies, and autoimmune diseases, but genetic manipulation of human T cells has been challenging. Improved tools are needed to efficiently “knock out” genes and “knock in” targeted genome modifications to modulate T-cell function and correct disease-associated mutations. CRISPR/Cas9 technology is facilitating genome engineering in many cell types, but in human T cells its efficiency has been limited and it has not yet proven useful for targeted nucleotide replacements. Here we report efficient genome engineering in human CD4+ T cells using Cas9:single-guide RNA ribonucleoproteins (Cas9 RNPs). Cas9more » RNPs allowed ablation of CXCR4, a coreceptor for HIV entry. Cas9 RNP electroporation caused up to ~40% of cells to lose high-level cell-surface expression of CXCR4, and edited cells could be enriched by sorting based on low CXCR4 expression. Importantly, Cas9 RNPs paired with homology-directed repair template oligonucleotides generated a high frequency of targeted genome modifications in primary T cells. Targeted nucleotide replacement was achieved in CXCR4 and PD-1 (PDCD1), a regulator of T-cell exhaustion that is a validated target for tumor immunotherapy. Deep sequencing of a target site confirmed that Cas9 RNPs generated knock-in genome modifications with up to ~20% efficiency, which accounted for up to approximately one-third of total editing events. These results establish Cas9 RNP technology for diverse experimental and therapeutic genome engineering applications in primary human T cells.« less

  6. The effect of shock dynamics on compressibility of ignition-scale National Ignition Facility implosions

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Zylstra, A. B.; Frenje, J. A.; Séguin, F. H.; Hicks, D. G.; Dewald, E. L.; Robey, H. F.; Rygg, J. R.; Meezan, N. B.; Rosenberg, M. J.; Rinderknecht, H. G.; et al

    2014-11-03

    The effects of shock dynamics on compressibility of indirect-drive ignition-scale surrogate implosions, CH shells filled with D3He gas, have been studied using charged-particle spectroscopy. Spectral measurements of D3He protons produced at the shock-bang time probe the shock dynamics and in-flight characteristics of an implosion. The proton shock yield is found to vary by over an order of magnitude. A simple model relates the observed yield to incipient hot-spot adiabat, suggesting that implosions with rapid radiation-power increase during the main drive pulse may have a 2x higher hot-spot adiabat, potentially reducing compressibility. A self-consistent 1-D implosion model was used to infermore » the areal density (pR) and the shell center-of-mass radius (Rcm) from the downshift of the shock-produced D3He protons. The observed pR at shock-bang time is substantially higher for implosions, where the laser drive is on until near the compression bang time ('short-coast'), while longer-coasting implosions have lower pR. This corresponds to a much larger temporal difference between the shock- and compression-bang time in the long-coast implosions (~800 ps) than in the short-coast (~400 ps); this will be verified with a future direct bang-time diagnostic. This model-inferred differential bang time contradicts radiation-hydrodynamic simulations, which predict constant 700–800 ps differential independent of coasting time. This result is potentially explained by uncertainties in modeling late-time ablation drive on the capsule. In an ignition experiment, an earlier shock-bang time resulting in an earlier onset of shell deceleration, potentially reducing compression and, thus, fuel pR.« less

  7. Topological evolution and photoluminescent properties of a series of divalent zinc-based metalorganic frameworks tuned via ancillary ligating spacers

    SciTech Connect (OSTI)

    Lian, Xiao-Min; Zhao, Wen; Zhao, Xiao-Li

    2013-04-15

    The combination of divalent zinc ions, 4-(4-carboxybenzamido)benzoic acid and exo-bidendate bipyridine ligands gave rise to a series of new MOFs: [ZnL(bipy)]DMFH{sub 2}O (1), [ZnL(bpe)]1.5H{sub 2}O (2), [ZnL(bpa)]4H{sub 2}O (3) and [ZnL(bpp)]1.75H{sub 2}O (4) (MOF=metal-organic framework, bipy=4,4?-bipyridine, bpe=trans-1,2-bis(4-pyridyl)ethylene, bpa=1,2-bis(4-pyridinyl)ethane, bpp=1,3-bis(4-pyridinyl)propane, H{sub 2}L=4,4?-(carbonylimino)dibenzoic acid). Fine tune over the topology of the MOFs was achieved via systematically varying the geometric length of the second ligating bipyridine ligands. Single-crystal X-ray analysis reveals that complex 1 has a triply interpenetrated three-dimensional (3D) framework with elongated primitive cubic topology, whereas isostructural complexes 2 and 3 each possesses a 6-fold interpenetrated diamondiod 3D framework. Further expansion of the length of the bipyridine ligand to bpp leads to the formation of 4, which features an interesting entangled architecture of 2D?3D parallel polycatenation. In addition, the thermogravimetric analyses and solid-state photoluminescent properties of the selected complexes are investigated. - Graphical abstract: The incorporation of exo-bidendate bipyridine spacers into the ZnH{sub 2}L system has yielded a series of new MOFs exhibiting topological evolution from 3-fold interpenetration to 6-fold interpenetration and 2D?3D parallel polycatenation. Highlights: ? The effect of the pyridyl-based spacers on the formation of MOFs was explored. ? Fine tune over the topology of the MOFs was achieved. ? An interesting structure of 2D?3D parallel polycatenation is reported.

  8. Benchmarking density functional theory predictions of framework structures and properties in a chemically diverse test set of metal-organic frameworks

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Nazarian, Dalar; Ganesh, P.; Sholl, David S.

    2015-09-30

    We compiled a test set of chemically and topologically diverse Metal–Organic Frameworks (MOFs) with high accuracy experimentally derived crystallographic structure data. The test set was used to benchmark the performance of Density Functional Theory (DFT) functionals (M06L, PBE, PW91, PBE-D2, PBE-D3, and vdW-DF2) for predicting lattice parameters, unit cell volume, bonded parameters and pore descriptors. On average PBE-D2, PBE-D3, and vdW-DF2 predict more accurate structures, but all functionals predicted pore diameters within 0.5 Å of the experimental diameter for every MOF in the test set. The test set was also used to assess the variance in performance of DFT functionalsmore » for elastic properties and atomic partial charges. The DFT predicted elastic properties such as minimum shear modulus and Young's modulus can differ by an average of 3 and 9 GPa for rigid MOFs such as those in the test set. Moreover, we calculated the partial charges by vdW-DF2 deviate the most from other functionals while there is no significant difference between the partial charges calculated by M06L, PBE, PW91, PBE-D2 and PBE-D3 for the MOFs in the test set. We find that while there are differences in the magnitude of the properties predicted by the various functionals, these discrepancies are small compared to the accuracy necessary for most practical applications.« less

  9. A geometric level set model for ultrasounds analysis

    SciTech Connect (OSTI)

    Sarti, A.; Malladi, R.

    1999-10-01

    We propose a partial differential equation (PDE) for filtering and segmentation of echocardiographic images based on a geometric-driven scheme. The method allows edge-preserving image smoothing and a semi-automatic segmentation of the heart chambers, that regularizes the shapes and improves edge fidelity especially in presence of distinct gaps in the edge map as is common in ultrasound imagery. A numerical scheme for solving the proposed PDE is borrowed from level set methods. Results on human in vivo acquired 2D, 2D+time,3D, 3D+time echocardiographic images are shown.

  10. Control room habitability system review models

    SciTech Connect (OSTI)

    Gilpin, H. )

    1990-12-01

    This report provides a method of calculating control room operator doses from postulated reactor accidents and chemical spills as part of the resolution of TMI Action Plan III.D.3.4. The computer codes contained in this report use source concentrations calculated by either TACT5, FPFP, or EXTRAN, and transport them via user-defined flow rates to the control room envelope. The codes compute doses to six organs from up to 150 radionuclides (or 1 toxic chemical) for time steps as short as one second. Supporting codes written in Clipper assist in data entry and manipulation, and graphically display the results of the FORTRAN calculations. 7 refs., 22 figs.

  11. Microsoft Word - S06246_VP_Report

    Office of Legacy Management (LM)

    NFSS Vicinity Property T This page intentionally left blank Assessment Data Excerpt from Comprehensive Radiological Survey Off-Site Property T Niagara Falls Storage Site Appendix D NFSS Vicinity Property T Page D-1 This page intentionally left blank Appendix D NFSS Vicinity Property T Page D-2 Appendix D NFSS Vicinity Property T Page D-3 Appendix D NFSS Vicinity Property T Page D-4 Appendix D NFSS Vicinity Property T Page D-5 Appendix D NFSS Vicinity Property T Page D-6 Appendix D NFSS Vicinity

  12. Spontaneous Breaking of Scale Invariance in U(N) Chern-Simons Gauge Theories in Three Dimensions

    SciTech Connect (OSTI)

    Bardeen, William

    2014-10-24

    I explore the existence of a massive phase in a conformally invariant U(N) Chern-Simons gauge theories in D = 3 with matter fields in the fundamental representation. These models have attracted recent attention as being dual, in the conformal phase, to theories of higher spin gravity on AdS 4. Using the 1t Hooft large N expansion, exact solutions are obtained for scalar current correlators in the massive phase where the conformal symmetry is spontaneously broken. A massless dilaton appears as a composite state, and its properties are discussed. Solutions exist for matters field that are either bosons or fermions.

  13. Federal Acquisition Circular (FAC) 2005-72

    Office of Energy Efficiency and Renewable Energy (EERE)

    Attached for your information is a list of Federal Acquisition Regulation (FAR) amendments, published in the December 31, 2014 Federal Register at 78 FR 80368. This FAC issued 4 final rules. Please note that the final rule on Service Contracts Reporting Requirements is applicable to solicitations issued and contracts awarded on or after January 30, 2014. Contracting officers will modify existing indefinite-delivery contracts, on a bilateral basis in accordance with FAR 1.108(d)(3), within six months of the effective date of the final rule, if the remaining period of performance extends beyond October 1, 2013, and $2.5 million or more remains to be obligated.

  14. Spontaneous Breaking of Scale Invariance in U(N) Chern-Simons Gauge Theories in Three Dimensions

    SciTech Connect (OSTI)

    Bardeen, William A.

    2015-09-24

    I explore the existence of a massive phase in a conformally invariant U(N) Chern-Simons gauge theories in D = 3 with matter fields in the fundamental representation. These models have attracted recent attention as being dual, in the conformal phase, to theories of higher spin gravity on AdS 4. Using the 0t Hooft large N expansion, exact solutions are obtained for scalar current correlators in the massive phase where the conformal symmetry is spontaneously broken. A massless dilaton appears as a composite state, and its properties are discussed. Solutions exist for matters field that are either bosons or fermions.

  15. NMMSS News nov 2012.cdr

    National Nuclear Security Administration (NNSA)

    SAMS 8 Crosswalk to SAMS D-2/D-3 Updates Training Courses NMMSS Germantown Operations NMMSS Team Members NMMSS Due Dates 2012 NMMSS Calendar NMMSS Mailing Addresses SAMS 8, the most current version is available upon request. If you would like a copy of this software please request by E-Mail (NMMSS@nnsa.doe.gov), FAX (301-903-1998) or phone Greg Perry at (301-903-3656). Please provide the name and address of the person it is to be sent to as well as the RIS ID. November 2012 NMMSS 2012 Meeting

  16. Surface characterizatin of palladium-alumina sorbents for high-temperature capture of mercury and arsenic from fuel gas

    SciTech Connect (OSTI)

    Baltrus, J.P.; Granite, E.J.; Pennline, H.W.; Stanko, D.; Hamilton, H.; Rowsell, L.; Poulston, S.; Smith, A.; Chu, W.

    2010-01-01

    Coal gasification with subsequent cleanup of the resulting fuel gas is a way to reduce the impact of mercury and arsenic in the environment during power generation and on downstream catalytic processes in chemical production, The interactions of mercury and arsenic with PdlAl2D3 model thin film sorbents and PdlAh03 powders have been studied to determine the relative affinities of palladium for mercury and arsenic, and how they are affected by temperature and the presence of hydrogen sulfide in the fuel gas. The implications of the results on strategies for capturing the toxic metals using a sorbent bed are discussed.

  17. Papers and Presentations - 2014

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    4 / december Papers and Presentations - 2014 December Studying Ion Kinetic Effects in NIF Implosions The results of an investigation of the role of kinetic and multi-ion effects on NIF implosions were reported in a Physics of Plasmas paper published online on Dec. 29. The research focused on shock-driven deuterium (D2) and deuterium-helium-three (D3He) gas-filled "exploding-pusher" inertial confinement fusion (ICF) implosions at NIF to assess the impact of ion kinetic effects.

  18. app_d

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    DOE/EIS-0287 Idaho HLW & FD EIS Document 1, Darryl D. Siemer, Idaho Falls, ID Page 1 of 18 Document 1, Darryl D. Siemer, Idaho Falls, ID Page 2 of 18 - New Information - DOE/EIS-0287 D-2 Appendix D Document 1, Darryl D. Siemer, Idaho Falls, ID Page 3 of 18 Document 1, Darryl D. Siemer, Idaho Falls, ID Page 4 of 18 - New Information - D-3 DOE/EIS-0287 Idaho HLW & FD EIS Document 1, Darryl D. Siemer, Idaho Falls, ID Page 5 of 18 Document 1, Darryl D. Siemer, Idaho Falls, ID Page 6 of 18 -

  19. ORNL Evaluation of Electrabel Safety Cases for Doel 3 / Tihange 2: Final Report

    SciTech Connect (OSTI)

    Bass, Bennett Richard; Dickson, Terry L.; Gorti, Sarma B.; Klasky, Hilda B.; Nanstad, Randy K.; Sokolov, Mikhail A.; Williams, Paul T.; Server, W. L.

    2015-11-01

    Oak Ridge National Laboratory (ORNL) performed a detailed technical review of the 2015 Electrabel (EBL) Safety Cases prepared for the Belgium reactor pressure vessels (RPVs) at Doel 3 and Tihange 2 (D3/T2). The Federal Agency for Nuclear Control (FANC) in Belgium commissioned ORNL to provide a thorough assessment of the existing safety margins against cracking of the RPVs due to the presence of almost laminar flaws found in each RPV. Initial efforts focused on surveying relevant literature that provided necessary background knowledge on the issues related to the quasilaminar flaws observed in D3/T2 reactors. Next, ORNL proceeded to develop an independent quantitative assessment of the entire flaw population in the two Belgian reactors according to the American Society of Mechanical Engineers (ASME) Boiler and Pressure Vessel Code, Section XI, Appendix G, Fracture Toughness Criteria for Protection Against Failure, New York (1992 and 2004). That screening assessment of all EBL-characterized flaws in D3/T2 used ORNL tools, methodologies, and the ASME Code Case N-848, Alternative Characterization Rules for QuasiLaminar Flaws . Results and conclusions from the ORNL flaw acceptance assessments of D3/T2 were compared with those from the 2015 EBL Safety Cases. Specific findings of the ORNL evaluation of that part of the EBL structural integrity assessment focusing on stability of the flaw population subjected to primary design transients include the following: ORNL s analysis results were similar to those of EBL in that very few characterized flaws were found not compliant with the ASME (1992) acceptance criterion. ORNL s application of the more recent ASME Section XI (2004) produced only four noncompliant flaws, all due to LOCAs. The finding of a greater number of non-compliant flaws in the EBL screening assessment is due principally to a significantly more restrictive (conservative) criterion for flaw size acceptance used by EBL. ORNL s screening assessment results

  20. Argonne In-Flight Radioactive Ion Separator

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In-Flight Radioactive Ion Separator www.phy.anl.gov/airis B. B. Back, C. Dickerson, C. R. Hoffman, B. P. Kay, B. Mustapha, J. A. Nolen, P. Ostroumov, R. C. Pardo, K. E. Rehm, G. Savard, J. P. Schiffer, D. Seweryniak - Argonne National Laboratory S. Manikonda, M. Alcorta - Former Collaborators 2 ATLAS In-Flight Radioactive Beam Program ● Nuclear astrophysics ● (,p) & (p,) ● Fusion cross sections ● Single-particle structure ● (d,p) & (d, 3 He), etc. w/ HELIOS ● Impact of

  1. Office of the Chief Information Officer DOERM@hq.doe.gov

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    a Program Records Official (PRO) 1 to support their respective RM processes. PROs are senior-level officials with HQ and Field oversight, budgetary, and signature authority to approve records issues. PROs are appointed using DOE F 243.3, Records Contact Appointment, and are tracked and reported by the DOE RM Program. AUTHORITIES  Title 36, Chapter XII, Subchapter B, Part 1220, Subpart B, Section 1220.34(d)  DOE O 243.1B, Records Management Program, Paragraph 5d(3)(a) DUTIES &

  2. Microsoft Word - Agenda for IIP NOPR Public Workshop_3-22-2016

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Agenda For Integrated Interagency Pre-Application (IIP) Process Notice of Proposed Rulemaking Public Workshop March 22, 2016 1:00 PM Eastern Time Workshop Facilitator: Josh Smith Workshop Presenters: Meghan Conklin, Julie Smith, and Lynn Alexander Webinar Address: https://iser.webex.com/iser/onstage/g.php?MTID=edfa1833d3ad5014f2b91124860aeecc1 Phone Number (if not joining webinar): (650) 479-3208, access code 660 032 881 I. Introduction II. Background of Integrated Interagency Pre-Application

  3. Appendix D: Chapter 4 - Establish a Process

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    D April 2016 APPENDIX D: Chapter 4 - Establish a Process Appendix D-1: At-A-Glance - Owner Memorandum of Understanding (MOU) Appendix D-2: At-A-Glance - Request for Proposals (RFP) to Pre-Qualify ESCOs Appendix D-3: At-A-Glance - Final ESCO Selection Appendix D-4: At-A-Glance - ESCO Base Contract (Contract for Pre-Qualified ESCOs) Appendix D-5: At-A-Glance - Investment Grade Audit (IGA) and Project Proposal Contract Appendix D-6: At-A-Glance - Energy Savings Performance Contract Appendix D-7:

  4. Special Report: IG-0777

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Special Report Loan Guarantees for Innovative Energy Technologies DOE/IG-0777 September 2007 Department of Energy vvast~lngton, U L L U 3 d 3 S e p t e m b e r 1 9 , 2007 MEMORANDUM FOR THE SECRETARY FROM: Inspector General SUBSEC'T: INFORMATION: Special Repol-t on "Loan Guarantees for Iilnovative Energy 'Technologies" BACKGROUND 'Title XVlI of the Energy Policy Act of 2005 authorized the Departinent of Energy to provide loan guarantees for projects that "avoid, reduce, or

  5. U

    Office of Environmental Management (EM)

    DOE O 484.1 6-30-2014 Page Paragraph Changed To 7 5.i(3) to 5.i(2) ... human and/or animal subjects, the responsible program Secretarial Officer must ensure that DOE ... ... human and/or animal subjects, ensure that DOE ... 8 5.j(4)b from DHS consistent with DOE policy [see paragraph 5j(1)]. from DHS consistent with DOE policy [see paragraph 5k(3)]. 9 5.k(10) ... is required by this Order (paragraphs 4g and 4i). ... is required by this Order (paragraphs 4e and 4g). 11 6.d(3) ... but performed in

  6. L)HM+H)/&D)$&'()$#&*+,%-./%&

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    "$%&'()$#&*+,%-./%&!&0(1,.2,&+#&!%$3.-+4& 5676&8)".,9&8:;89&:"<",#&=9&>?@@& * &A+/#+-.B./<&C%,"-#,&D$)3&'()$#&*+,%-./%&!&EFG%$.3%/#,& * &A(%)$%H2+-&I/#%$G$%#+H)/,&)D&EF.,H/<&J+#+& * &!"#"$%&0$)G),%K&EFG%$.3%/#,&+#&!%$3.-+4& !"#$%&'()*+,-*)$ ..$/-)0*102-)$ 3,45)$,+&$2-$6&7$ 8,'&9$$

  7. Measurement of the 3s1/2-3p3/2 resonance line of sodiumlike Eu52+

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Träbert, E.; Beiersdorfer, P.; Hell, N.; Brown, G. V.

    2015-08-20

    We have measured the 3s1/2-3p3/2 transition in sodiumlike Eu52+ situated at 41.232 Å with an uncertainty of 73 ppm. Our measurement extends previous high-precision measurements into the 56< Z< 78 range of atomic numbers. We also present measurements of 3s1/2-3p3/2 and 3p1/2-3d3/2 transitions in the neighboring magnesiumlike, aluminumlike, and siliconlike europium ions.

  8. 2011-2012 SECTION II: HEAVY ION REACTIONS

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    A search for super heavy elements using a catcher foil M. Barbui, K. Schmidt, J.B. Natowitz, H. Zheng, K. Hagel, A. Bonasera and M. Barbino Probing clusterization in 40Ca + 40Ca reactions K. Schmidt, M. Barbui, J. B. Natowitz, K. Hagel, A. Bonasera, G. Giuliani, M. Rodrigues, R. Wada, M. Huang, C. Botosso, G. Liu, G. Viesti, S. Moretto, G. Prete, S. Pesente, D. Fabris, Y. El Masri, T. Keutgen, S. Kowalski, and A. Kumar Yield of D-D and D-3He fusion reactions produced by the interaction of

  9. I'

    Office of Legacy Management (LM)

    N.y.%2 ,.; 1 - VF :' ,. .., . ., .' !..' I' t ,f. : ._ : II . .."W ,.I , : 3~4k-~%d . !3cx #TO ._ I' _' >. ._ .:f$ -7 : _ ..:.>, .a ._ 5 &[&,v# 24+4&&3 7-&i 4 ?. 6. Bpp, chaof, Tonrrrrprda 8ub-offioo .-. bgwt16, 1980 L P. Y. Bolwro, D@oetos, Prodwtiam Dlriaicai, Bow York c MX-808 RM B&B BTLIBXB , 8YMmr mo8Dom8rr - 1' _, ~q,< Roforonao ir wdo' to ymr amno- 'oononminc; (L qrorrrttty af hpw-'b'lub arida Vndor It8 domestio wanltr pmgrk, OIo Carirrloa bir

  10. IVASI I-

    Office of Legacy Management (LM)

    IVASI I- /& 1 -i /' / . /' A 1:' *' , tui POR-I RAI)IOI,OGICAI, sLlRvt:Y 01: 1' IlE SIblO?!DS s,w c STEEL Co!4'~Is'l R. W . Leggctt , J . Burden, bl. T. Ryan, D. L. Anderson, B. S. Ellis, and D. 3. Christian W o rk performed Health Physics Divisidn OAK RIDGE NATIOI\TAL LABOR4TORY Oak Ridge, Tennessee FEBRUARY 1977 for U. S. ENERGY RESEARCH AND DEVELOPblENT ADFIINISTRATION as part of The Radiological Surveys of Former Manhattan Engineering District/ Atomic Energy Commission Sites By .cc.ptmc.

  11. Energy Literacy Social Studies Guides

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Where does our food come from? Energy Literacy Essential Principle 3: Biological processes depend on energy flow through the Earth system. C3 Framework for Social Studies Focus Indicators D1: Identify facts and concepts associated with a supporting question. (D1.3.K-2) D2: Use maps, graphs, photographs, and other representations to describe places and the relationships and interactions that shape them. (D2.Geo.2.K-2) D3: Gather relevant information from one or two sources while using the origin

  12. Energy Literacy Social Studies Guides

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    does transportation impact the environment? Energy Literacy Essential Principle 7: The quality of life of individuals and societies is affected by energy choices. C3 Framework for Social Studies Focus Indicators D1: Identify disciplinary ideas associated with a compelling question. (D1.2.K-2) D2: Identify the benefits and costs of making various personal decisions. (D2.Eco.2.K-2) D3: Gather relevant information from one or two sources while using the origin and structure to guide the selection.

  13. K9/9J)$&95LL"4-5&&

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Studies of exotic proton-rich nuclei !"#"$%&'%()*+&,-./$$"&0%1/$%#&2%3/-%4/-5& ,62,7&89"-&:""1$.&;< 4= >;? 4= &:%5&@A;B& !"#$%#$&'()%#*%#$+%,-./0.--.123454%6*77&8*9&:*';% ,-./$$"&0%1/$%#&2%3/-%4/-5& 8$)("-9)45&/C&D*)$3E-.=& 8$)("-9)45&/C&:%-5#%$*&&

  14. Differentiation of C2C12 myoblasts expressing lamin A mutated at a site responsible for Emery-Dreifuss muscular dystrophy is improved by inhibition of the MEK-ERK pathway and stimulation of the PI3-kinase pathway

    SciTech Connect (OSTI)

    Favreau, Catherine; Delbarre, Erwan; Courvalin, Jean-Claude; Buendia, Brigitte

    2008-04-01

    Mutation R453W in A-type lamins, that are major nuclear envelope proteins, generates Emery-Dreifuss muscular dystrophy. We previously showed that mouse myoblasts expressing R453W-lamin A incompletely exit the cell cycle and differentiate into myocytes with a low level of multinucleation. Here we attempted to improve differentiation by treating these cells with a mixture of PD98059, an extracellular-regulated kinase (ERK) kinase (also known as mitogen-activated kinase, MEK) inhibitor, and insulin-like growth factor-II, an activator of phosphoinositide 3-kinase. We show that mouse myoblasts expressing R453W-lamin A were sensitive to the drug treatment as shown by (i) an increase in multinucleation, (ii) downregulation of proliferation markers (cyclin D1, hyperphosphorylated Rb), (iii) upregulation of myogenin, and (iv) sustained activation of p21 and cyclin D3. However, nuclear matrix anchorage of p21 and cyclin D3 in a complex with hypophosphorylated Rb that is critical to trigger cell cycle arrest and myogenin induction was deficient and incompletely restored by drug treatment. As the turn-over of R453W-lamin A at the nuclear envelope was greatly enhanced, we propose that R453W-lamin A impairs the capacity of the nuclear lamina to serve as scaffold for substrates of the MEK-ERK pathway and for MyoD-induced proteins that play a role in the differentiation process.

  15. Three-dimensional lattice Boltzmann model for magnetic reconnection

    SciTech Connect (OSTI)

    Mendoza, M.; Munoz, J. D.

    2008-02-15

    We develop a three-dimensional (3D) lattice Boltzmann model that recovers in the continuous limit the two-fluids theory for plasmas, and consequently includes the generalized Ohm's law. The model reproduces the magnetic reconnection process just by giving the right initial equilibrium conditions in the magnetotail, without any assumption on the resistivity in the diffusive region. In this model, the plasma is handled similar to two fluids with an interaction term, each one with distribution functions associated to a cubic lattice with 19 velocities (D3Q19). The electromagnetic fields are considered as a third fluid with an external force on a cubic lattice with 13 velocities (D3Q13). The model can simulate either viscous fluids in the incompressible limit or nonviscous compressible fluids, and successfully reproduces both the Hartmann flow and the magnetic reconnection in the magnetotail. The reconnection rate in the magnetotail obtained with this model lies between R=0.062 and R=0.073, in good agreement with the observations.

  16. A curvilinear, fully implicit, conservative electromagnetic PIC algorithm in multiple dimensions

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Chacon, L.; Chen, G.

    2016-04-19

    Here, we extend a recently proposed fully implicit PIC algorithm for the Vlasov–Darwin model in multiple dimensions (Chen and Chacón (2015) [1]) to curvilinear geometry. As in the Cartesian case, the approach is based on a potential formulation (Φ, A), and overcomes many difficulties of traditional semi-implicit Darwin PIC algorithms. Conservation theorems for local charge and global energy are derived in curvilinear representation, and then enforced discretely by a careful choice of the discretization of field and particle equations. Additionally, the algorithm conserves canonical-momentum in any ignorable direction, and preserves the Coulomb gauge ∇ • A = 0 exactly. Anmore » asymptotically well-posed fluid preconditioner allows efficient use of large cell sizes, which are determined by accuracy considerations, not stability, and can be orders of magnitude larger than required in a standard explicit electromagnetic PIC simulation. We demonstrate the accuracy and efficiency properties of the algorithm with numerical experiments in mapped meshes in 1D-3V and 2D-3V.« less

  17. Study of the negative ion extraction mechanism from a double-ion plasma in negative ion sources

    SciTech Connect (OSTI)

    Goto, I.; Nishioka, S.; Hatayama, A.; Miyamoto, K.

    2015-04-08

    We have developed a 2D3V-PIC model of the extraction region, aiming to clarify the basic extraction mechanism of H{sup −} ions from the double-ion plasma in H{sup −} negative ion sources. The result shows the same tendency of the H{sup −} ion density n{sub H{sup −}} as that observed in the experiments, i.e.,n{sub H{sup −}} in the upstream region away from the plasma meniscus (H{sup −} emitting surface) has been reduced by applying the extraction voltage. At the same time, relatively slow temporal oscillation of the electric potential compared with the electron plasma frequency has been observed in the extraction region. Results of the systematic study using a 1D3V-PIC model with the uniform magnetic field confirm the result that the electrostatic oscillation is identified to be lower hybrid wave. The effect of this oscillation on the H{sup −} transport will be studied in the future.

  18. Using multiple secondary fusion products to evaluate fuel ρR, electron temperature, and mix in deuterium-filled implosions at the NIF

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Rinderknecht, H. G.; Rosenberg, M. J.; Zylstra, A. B.; Lahmann, B.; Séguin, F. H.; Frenje, J. A.; Li, C. K.; Gatu Johnson, M.; Petrasso, R. D.; Berzak Hopkins, L. F.; et al

    2015-08-25

    In deuterium-filled inertial confinement fusion implosions, the secondary fusion processes D(3He,p)4He and D(T,n)4He occur, as the primary fusion products 3He and T react in flight with thermal deuterons. In implosions with moderate fuel areal density (~ 5–100 mg/cm2), the secondary D-3He reaction saturates, while the D-T reaction does not, and the combined information from these secondary products is used to constrain both the areal density and either the plasma electron temperature or changes in the composition due to mix of shell material into the fuel. The underlying theory of this technique is developed and applied to three classes of implosionsmore » on the National Ignition Facility: direct-drive exploding pushers, indirect-drive 1-shock and 2-shock implosions,and polar direct-drive implosions. In the 1- and 2-shock implosions, the electron temperature is inferred to be 0.65 x and 0.33 x the burn-averaged ion temperature, respectively. The inferred mixed mass in the polar direct-drive implosions is in agreement with measurements using alternative techniques.« less

  19. In-flight observations of low-mode ρR asymmetries in NIF implosions

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Zylstra, A. B.; Frenje, J. A.; Seguin, F. H.; Rygg, J. R.; Kritcher, A.; Rosenberg, M. J.; Rinderknecht, H. G.; Hicks, D. G.; Friedrich, S.; Bionta, R.; et al

    2015-05-01

    Charged-particle spectroscopy is used to assess implosion symmetry in ignition-scale indirect-drive implosions for the first time. Surrogate D3He gas-filled implosions at the National Ignition Facility produce energetic protons via D+3He fusion that are used to measure the implosion areal density (ρR) at the shock-bang time. By using protons produced several hundred ps before the main compression bang, the implosion is diagnosed in-flight at a convergence ratio of 3-5 just prior to peak velocity. This isolates acceleration-phase asymmetry growth. For many surrogate implosions, proton spectrometers placed at the north pole and equator reveal significant asymmetries with amplitudes routinely ≳10%, which aremore » interpreted as l=2 Legendre modes. With significant expected growth by stagnation, it is likely that these asymmetries would degrade the final implosion performance. X-ray self-emission images at stagnation show asymmetries that are positively correlated with the observed in-flight asymmetries and comparable in magnitude, contradicting growth models; this suggests that the hot-spot shape does not reflect the stagnated shell shape or that significant residual kinetic energy exists at stagnation. More prolate implosions are observed when the laser drive is sustained (“no-coast”), implying a significant time-dependent asymmetry in peak drive.« less

  20. A direct-drive exploding-pusher implosion as the first step in development of a monoenergetic charged-particle backlighting platforn at the National Ignition Facility

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Rosenberg, M. J.; Zylstra, A. B.; Seguin, F. H.; Rinderknecht, H. G.; Frenje, J. A.; Johnson, M. Gatu; Sio, H.; Waugh, C. J.; Li, C. K.; Petrasso, R. D.; et al

    2016-01-18

    A thin-glass-shell, D3He-filled exploding-pusher inertial confinement fusion implosion at the National Ignition Facility (NIF) has been demonstrated as a proton source that serves as a promising first step toward development of a monoenergetic proton, alpha, and triton backlighting platform at the NIF. Among the key measurements, the D3He-proton emission on this experiment (shot N121128) has been well-characterized spectrally, temporally, and in terms of emission isotropy, revealing a highly monoenergetic (ΔE/E~4%) and isotropic source (~3% proton fluence variation and ~0.5% proton energy variation). On a similar shot (N130129, with D2 fill), the DD-proton spectrum has been obtained as well, illustrating thatmore » monoenergetic protons of multiple energies may be utilized in a single experiment. In conclusion, these results, and experiments on OMEGA, point toward future steps in the development of a precision, monoenergetic proton, alpha, and triton source that can readily be implemented at the NIF for backlighting a broad range of high energy density physics (HEDP) experiments in which fields and flows are manifest, and also utilized for studies of stopping power in warm dense matter and in classical plasmas.« less

  1. Energy levels, oscillator strengths and transition probabilities for Si-like P II, S III, Cl IV, Ar V and K VI

    SciTech Connect (OSTI)

    Abou El-Maaref, A.; Uosif, M.A.M.; Allam, S.H.; El-Sherbini, Th.M.

    2012-07-15

    Fine-structure calculations of energy levels, oscillator strengths, and transition probabilities for transitions among the terms belonging to 3s{sup 2}3p{sup 2}, 3s3p{sup 3}, 3s{sup 2}3p3d, 3s{sup 2}3p4s, 3s{sup 2}3p4p, 3s{sup 2}3p4d, 3s{sup 2}3p5s and 3s{sup 2}3p5p configurations of silicon-like ions P II, S III, Cl IV, Ar V and K VI have been calculated using configuration-interaction version 3 (CIV3). We compared our data with the available experimental data and other theoretical calculations. Most of our calculations of energy levels and oscillator strengths (in length form) show good agreement with both experimental and theoretical data. Lifetimes of the excited levels are also given.

  2. Human non-neutralizing HIV-1 envelope monoclonal antibodies limit the number of founder viruses during SHIV mucosal infection in rhesus macaques

    SciTech Connect (OSTI)

    Santra, Sampa; Tomaras, Georgia D.; Warrier, Ranjit; Nicely, Nathan I.; Liao, Hua -Xin; Pollara, Justin; Liu, Pinghuang; Alam, S. Munir; Zhang, Ruijun; Cocklin, Sarah L.; Shen, Xiaoying; Duffy, Ryan; Xia, Shi -Mao; Schutte, Robert J.; Pemble IV, Charles W.; Dennison, S. Moses; Li, Hui; Chao, Andrew; Vidnovic, Kora; Evans, Abbey; Klein, Katja; Kumar, Amit; Robinson, James; Landucci, Gary; Forthal, Donald N.; Montefiori, David C.; Kaewkungwal, Jaranit; Nitayaphan, Sorachai; Pitisuttithum, Punnee; Rerks-Ngarm, Supachai; Robb, Merlin L.; Michael, Nelson L.; Kim, Jerome H.; Soderberg, Kelly A.; Giorgi, Elena E.; Blair, Lily; Korber, Bette T.; Moog, Christiane; Shattock, Robin J.; Letvin, Norman L.; Schmitz, Joern E.; Moody, M. A.; Gao, Feng; Ferrari, Guido; Shaw, George M.; Haynes, Barton F.; Douek, Daniel C.

    2015-08-03

    HIV-1 mucosal transmission begins with virus or virus-infected cells moving through mucus across mucosal epithelium to infect CD4⁺ T cells. Although broadly neutralizing antibodies (bnAbs) are the type of HIV-1 antibodies that are most likely protective, they are not induced with current vaccine candidates. In contrast, antibodies that do not neutralize primary HIV-1 strains in the TZM-bl infection assay are readily induced by current vaccine candidates and have also been implicated as secondary correlates of decreased HIV-1 risk in the RV144 vaccine efficacy trial. Here, we have studied the capacity of anti-Env monoclonal antibodies (mAbs) against either the immunodominant region of gp41 (7B2 IgG1), the first constant region of gp120 (A32 IgG1), or the third variable loop (V3) of gp120 (CH22 IgG1) to modulate in vivo rectal mucosal transmission of a high-dose simian-human immunodeficiency virus (SHIV-BaL) in rhesus macaques. 7B2 IgG1 or A32 IgG1, each containing mutations to enhance Fc function, was administered passively to rhesus macaques but afforded no protection against productive clinical infection while the positive control antibody CH22 IgG1 prevented infection in 4 of 6 animals. Enumeration of transmitted/founder (T/F) viruses revealed that passive infusion of each of the three antibodies significantly reduced the number of T/F genomes. Some antibodies that bind HIV-1 Env but fail to neutralize virus in traditional neutralization assays may limit the number of T/F viruses involved in transmission without leading to enhancement of viral infection. For one of these mAbs, gp41 mAb 7B2, we provide the first co-crystal structure in complex with a common cyclical loop motif demonstrated to be critical for infection by other retroviruses.

  3. Development of Biomarkers for Chronic Beryllium Disease in Mice

    SciTech Connect (OSTI)

    Gordon, Terry

    2013-01-25

    changes in potential protein biomarkers in beryllium-treated mice. We will correlate these findings with the ability of the transgenic mice to develop a beryllium-specific adaptive immune response in blood and bronchoalveolar lavage (BAL) fluid. We will also determine whether beryllium-responsive CD4+ T cells in blood and BAL correlate with the onset of granuloma formation. Thus, we will provide the scientific community with biomarkers of sensitization and disease progression for CBD. These biomarkers will serve as critical tools for development of improved industrial hygiene and therapeutic interventions.

  4. Oral administration of drugs with hypersensitivity potential induces germinal center hyperplasia in secondary lymphoid organ/tissue in Brown Norway rats, and this histological lesion is a promising candidate as a predictive biomarker for drug hypersensitivity occurrence in humans

    SciTech Connect (OSTI)

    Tamura, Akitoshi Miyawaki, Izuru; Yamada, Toru; Kimura, Juki; Funabashi, Hitoshi

    2013-08-15

    cells and IgE, but the others did not. The allergic drugs commonly induced germinal center hyperplasia in lymphoid tissues. Some of these allergic drugs transiently increased CD4{sup +}CD25{sup +} T cells in the spleen.

  5. CYP2E1-dependent and leptin-mediated hepatic CD57 expression on CD8 + T cells aid progression of environment-linked nonalcoholic steatohepatitis

    SciTech Connect (OSTI)

    Seth, Ratanesh Kumar; Das, Suvarthi; Kumar, Ashutosh; Chanda, Anindya; Kadiiska, Maria B.; Michelotti, Gregory; Manautou, Jose; Diehl, Anna Mae; Chatterjee, Saurabh

    2014-01-01

    Environmental toxins induce a novel CYP2E1/leptin signaling axis in liver. This in turn activates a poorly characterized innate immune response that contributes to nonalcoholic steatohepatitis (NASH) progression. To identify the relevant subsets of T-lymphocytes in CYP2E1-dependent, environment-linked NASH, we utilized a model of diet induced obese (DIO) mice that are chronically exposed to bromodichloromethane. Mice deficient in CYP2E1, leptin (ob/ob mice), or both T and B cells (Pfp/Rag2 double knockout (KO) mice) were used to delineate the role of each of these factors in metabolic oxidative stress-induced T cell activation. Results revealed that elevated levels of lipid peroxidation, tyrosyl radical formation, mitochondrial tyrosine nitration and hepatic leptin as a consequence of metabolic oxidative stress caused increased levels of hepatic CD57, a marker of peripheral blood lymphocytes including NKT cells. CD8 + CD57 + cytotoxic T cells but not CD4 + CD57 + cells were significantly decreased in mice lacking CYP2E1 and leptin. There was a significant increase in the levels of T cell cytokines IL-2, IL-1?, and IFN-? in bromodichloromethane exposed DIO mice but not in mice that lacked CYP2E1, leptin or T and B cells. Apoptosis as evidenced by TUNEL assay and levels of cleaved caspase-3 was significantly lower in leptin and Pfp/Rag2 KO mice and highly correlated with protection from NASH. The results described above suggest that higher levels of oxidative stress-induced leptin mediated CD8 + CD57 + T cells play an important role in the development of NASH. It also provides a novel insight of immune dysregulation and may be a key biomarker in NASH. - Highlights: Metabolic oxidative stress caused increased levels of hepatic CD57 expression. CD8+ CD57+ cytotoxic T cells were decreased in mice lacking CYP2E1 and leptin. There was a significant increase in T cell cytokines in toxin-treated mice. Apoptosis was significantly lower in leptin and Pfp/Rag2 KO mice

  6. Human non-neutralizing HIV-1 envelope monoclonal antibodies limit the number of founder viruses during SHIV mucosal infection in rhesus macaques

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Santra, Sampa; Tomaras, Georgia D.; Warrier, Ranjit; Nicely, Nathan I.; Liao, Hua -Xin; Pollara, Justin; Liu, Pinghuang; Alam, S. Munir; Zhang, Ruijun; Cocklin, Sarah L.; et al

    2015-08-03

    HIV-1 mucosal transmission begins with virus or virus-infected cells moving through mucus across mucosal epithelium to infect CD4⁺ T cells. Although broadly neutralizing antibodies (bnAbs) are the type of HIV-1 antibodies that are most likely protective, they are not induced with current vaccine candidates. In contrast, antibodies that do not neutralize primary HIV-1 strains in the TZM-bl infection assay are readily induced by current vaccine candidates and have also been implicated as secondary correlates of decreased HIV-1 risk in the RV144 vaccine efficacy trial. Here, we have studied the capacity of anti-Env monoclonal antibodies (mAbs) against either the immunodominant regionmore » of gp41 (7B2 IgG1), the first constant region of gp120 (A32 IgG1), or the third variable loop (V3) of gp120 (CH22 IgG1) to modulate in vivo rectal mucosal transmission of a high-dose simian-human immunodeficiency virus (SHIV-BaL) in rhesus macaques. 7B2 IgG1 or A32 IgG1, each containing mutations to enhance Fc function, was administered passively to rhesus macaques but afforded no protection against productive clinical infection while the positive control antibody CH22 IgG1 prevented infection in 4 of 6 animals. Enumeration of transmitted/founder (T/F) viruses revealed that passive infusion of each of the three antibodies significantly reduced the number of T/F genomes. Some antibodies that bind HIV-1 Env but fail to neutralize virus in traditional neutralization assays may limit the number of T/F viruses involved in transmission without leading to enhancement of viral infection. For one of these mAbs, gp41 mAb 7B2, we provide the first co-crystal structure in complex with a common cyclical loop motif demonstrated to be critical for infection by other retroviruses.« less

  7. Real time in situ detection of organic nitrates in atmospheric aerosols

    SciTech Connect (OSTI)

    Rollins, Andrew W.; Smith, Jared D.; Wilson, Kevin R.; Cohen, Ronald C.

    2010-06-11

    A new field instrument is described that quantifies total particle phase organic nitrates. The instrument is based on the thermal dissociation laser induced fluorescence (TD-LIF) method that thermally converts nitrates to NO2 which is then detected by LIF. This instrument is unique in its ability to provide fast sensitive measurements of particle phase organic nitrates, without interference from inorganic nitrate. Here we use it to quantify organic nitrates in SOA generated from high-NOx photooxidation of limonene, a-pinene, D-3-carene, and tridecane. In these experiments the organic nitrate moiety is observed to be 6-15percent of the total SOA mass, depending on the organic precursor.

  8. Fragility of ferromagnetic double exchange interactions and pressure tuning of magnetism in 3d–5d double perovskite Sr₂FeOsO₆

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Veiga, L. S. I.; Fabbris, G.; van Veenendaal, M.; Souza-Neto, N. M.; Feng, H. L.; Yamaura, K.; Haskel, D.

    2015-06-19

    The ability to tune exchange (magnetic) interactions between 3d transition metals in perovskite structures has proven to be a powerful route to discovery of novel properties. Here we demonstrate that the introduction of 3d-5d exchange pathways in double perovskites enables additional tunability, a result of the large spatial extent of 5d wave functions. Using x-ray probes of magnetism and structure at high pressure, we show that compression of Sr₂FeOsO₆ drives an unexpected continuous change in the sign of Fe-Os exchange interactions and a transition from antiferromagnetic to ferrimagnetic order. We analyze the relevant electron-electron interactions, shedding light into fundamental differencesmore » with the more thoroughly studied 3d-3d systems.« less

  9. Workshop Goals, Objectives, and Desired Outcomes

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    D presentation slides: Workshop Goals, objectives, and Desired outcomes Steve Chalk, DOE 1 OctOber 2011 | ArgOnne nAtiOnAl lAbOrAtOry NG Workshop summary report - appeNDIX D 2 OctOber 2011 | ArgOnne nAtiOnAl lAbOrAtOry NG Workshop summary report - appeNDIX D 3 OctOber 2011 | ArgOnne nAtiOnAl lAbOrAtOry NG Workshop summary report - appeNDIX D 4 OctOber 2011 | ArgOnne nAtiOnAl lAbOrAtOry NG Workshop summary report - appeNDIX D 5 OctOber 2011 | ArgOnne nAtiOnAl lAbOrAtOry NG Workshop summary report

  10. Atomic Physics Effects on Convergent, Child-Langmuir Ion Flow between Nearly Transparent Electrodes

    SciTech Connect (OSTI)

    Santarius, John F.; Emmert, Gilbert A.

    2013-11-07

    Research during this project at the University of Wisconsin Fusion Technology Institute (UW FTI) on ion and neutral flow through an arbitrary, monotonic potential difference created by nearly transparent electrodes accomplished the following: (1) developed and implemented an integral equation approach for atomic physics effects in helium plasmas; (2) extended the analysis to coupled integral equations that treat atomic and molecular deuterium ions and neutrals; (3) implemented the key deuterium and helium atomic and molecular cross sections; (4) added negative ion production and related cross sections; and (5) benchmarked the code against experimental results. The analysis and codes treat the species D0, D20, D+, D2+, D3+, D and, separately at present, He0 and He+. Extensions enhanced the analysis and related computer codes to include He++ ions plus planar and cylindrical geometries.