National Library of Energy BETA

Sample records for binds dashed line

  1. DASH | Open Energy Information

    Open Energy Info (EERE)

    Interface: Website, Mobile Device Website: dash.by Web Application Link: dash.by Cost: Free OpenEI Keyword(s): Featured, Challenge Generated DASH Screenshot References: DASH1...

  2. Valve and dash-pot assembly

    DOE Patents [OSTI]

    Chang, Shih-Chih (Richland, WA)

    1986-01-01

    A dash-pot valve comprising a cylinder submerged in the fluid of a housing and having a piston attached to a plunger projecting into the path of closing movement of a pivotal valve member. A vortex chamber in said cylinder is provided with tangentially directed inlets to generate vortex flow upon retraction of said plunger and effect increasing resistance against said piston to progressively retard the closing rate of said valve member toward its seat.

  3. Improved valve and dash-pot assembly

    DOE Patents [OSTI]

    Chang, S.C.

    1985-04-23

    A dash-pot valve comprises a cylinder submerged in the fluid of a housing and have a piston attached to a plunger projecting into the path of closing movement of a pivotal valve member. A vortex chamber in said cylinder is provided with targentially directed inlets to generate vortex flow upon retraction of said plunger and effect increasing resistance against said piston to progressively retard the closing rate of said valve member toward its seat.

  4. Development Wells At Fenton Hill HDR Geothermal Area (Dash, Et...

    Open Energy Info (EERE)

    16, 1979. References Z. V. Dash, H. D. Murphy, R. L. Aamodt, R. G. Aguilar, D. W. Brown, D. A. Counce, H. N. Fisher, C. O. Grigsby, H. Keppler, A. W. Laughlin, R. M. Potter,...

  5. Dash for Bikes, Walk for Trikes | National Nuclear Security Administration

    National Nuclear Security Administration (NNSA)

    Dash for Bikes, Walk for Trikes | National Nuclear Security Administration Facebook Twitter Youtube Flickr RSS People Mission Managing the Stockpile Preventing Proliferation Powering the Nuclear Navy Emergency Response Recapitalizing Our Infrastructure Countering Nuclear Terrorism About Our Programs Our History Who We Are Our Leadership Our Locations Budget Our Operations Library Bios Congressional Testimony Fact Sheets Newsletters Press Releases Photo Gallery Jobs Apply for Our Jobs Our Jobs

  6. Flow Test At Fenton Hill HDR Geothermal Area (Dash, Et Al., 1983...

    Open Energy Info (EERE)

    Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Flow Test At Fenton Hill HDR Geothermal Area (Dash, Et Al., 1983) Exploration Activity Details...

  7. Flow Test At Fenton Hill HDR Geothermal Area (Dash, 1989) | Open...

    Open Energy Info (EERE)

    Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Flow Test At Fenton Hill HDR Geothermal Area (Dash, 1989) Exploration Activity Details Location...

  8. Exciton and biexciton dynamics in single self-assembled InAs/InGaAlAs/InP quantum dash emitting near 1.55??m

    SciTech Connect (OSTI)

    Dusanowski, ?.; Syperek, M. Rudno-Rudzi?ski, W.; Mrowi?ski, P.; Sek, G.; Misiewicz, J.; Somers, A.; Reithmaier, J. P.; Höfling, S.; Forchel, A.

    2013-12-16

    Exciton and biexciton dynamics in a single self-assembled InAs/In{sub 0.53}Ga{sub 0.23}Al{sub 0.24}As/InP(001) quantum dash emitting near 1.55??m has been investigated by micro-photoluminescence and time-resolved micro-photoluminescence at T?=?4.2?K. The exciton and biexciton fine structure splitting of ?60??eV, the biexciton binding energy of ?3.5?meV, and the characteristic exciton and biexciton decay times of 2.0?±?0.1?ns and 1.1?±?0.1?ns, respectively, have been determined. The measurement of the biexciton and exciton cross-correlation statistics of the photon emission confirmed the cascaded relaxation process. The exciton-to-biexciton decay time ratio and a small fine structure splitting suggest carrier localization within the investigated quantum dash.

  9. Particle trap to sheath non-binding contact for a gas-insulated transmission line having a corrugated outer conductor

    DOE Patents [OSTI]

    Fischer, William H. (Pittsburgh, PA)

    1984-04-24

    A non-binding particle trap to outer sheath contact for use in gas insulated transmission lines having a corrugated outer conductor. The non-binding feature of the contact according to the teachings of the invention is accomplished by having a lever arm rotatably attached to a particle trap by a pivot support axis disposed parallel to the direction of travel of the inner conductor/insulator/particle trap assembly.

  10. Non-binding conductor load bearing roller for a gas-insulated transmission line having a corrugated outer conductor

    DOE Patents [OSTI]

    Fischer, William H. (Pittsburgh, PA)

    1984-01-01

    A gas-insulated transmission line includes a corrugated outer conductor, an inner conductor disposed within and insulated from the outer conductor by means of support insulators and an insulating gas, and a non-binding transport device for supporting and permitting movement of the inner conductor/insulating support assembly axially along the corrugated outer conductor without radial displacement and for moving without binding along corrugations of any slope less than vertical. The transport device includes two movable contacts, such as skids or rollers, supported on a common pivot lever, the pivot lever being rotatably disposed about a pivot lever axis, which pivot lever axis is in turn disposed on the periphery of a support insulator or particle trap if one is used. The movable contacts are separated axially a distance equal to the axial distance between the peaks and valleys of the corrugations of the outer conductor and separated radially a distance equal to the radial distance between the peaks and valleys of the corrugations of the outer conductor. The transport device has the pivot lever axis disposed parallel to the motion of travel of the inner conductor/insulating support assembly.

  11. Single photon emission at 1.55??m from charged and neutral exciton confined in a single quantum dash

    SciTech Connect (OSTI)

    Dusanowski, ?. Syperek, M.; Mrowi?ski, P.; Rudno-Rudzi?ski, W.; Misiewicz, J.; S?k, G.; Somers, A.; Kamp, M.; Höfling, S.; Reithmaier, J. P.

    2014-07-14

    We investigate charged and neutral exciton complexes confined in a single self-assembled InAs/InGaAlAs/InP quantum dash emitting at 1.55??m. The emission characteristics have been probed by measuring high-spatial-resolution polarization-resolved photoluminescence and cross-correlations of photon emission statistics at T?=?5?K. The photon auto-correlation histogram of the emission from both the neutral and charged exciton indicates a clear antibunching dip with as-measured g{sup (2)}(0) values of 0.18 and 0.31, respectively. It proves that these exciton complexes confined in single quantum dashes of InP-based material system can act as true single photon emitters being compatible with standard long-distance fiber communication technology.

  12. Line Projects

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Grand Coulee Transmission Line Replacement Project Hooper Springs McNary-John Day Montana-to-Washington Transmission System Upgrade Project - M2W Olympia-Grand Coulee No. 1...

  13. Inhibition of selectin binding

    DOE Patents [OSTI]

    Nagy, Jon O. (Rodeo, CA); Spevak, Wayne R. (Albany, CA); Dasgupta, Falguni (New Delhi, IN); Bertozzi, Caroline (Albany, CA)

    2001-10-09

    This invention provides compositions for inhibiting the binding between two cells, one expressing P- or L-selectin on the surface and the other expressing the corresponding ligand. A covalently crosslinked lipid composition is prepared having saccharides and acidic group on separate lipids. The composition is then interposed between the cells so as to inhibit binding. Inhibition can be achieved at an effective oligosaccharide concentration as low as 10.sup.6 fold below that of the free saccharide. Since selectins are involved in recruiting cells to sites of injury, these composition scan be used to palliate certain inflammatory and immunological conditions.

  14. Inhibition of selectin binding

    DOE Patents [OSTI]

    Nagy, Jon O. (Rodeo, CA); Spevak, Wayne R. (Albany, CA); Dasgupta, Falguni (New Delhi, IN); Bertozzi, Carolyn (Albany, CA)

    1999-10-05

    This invention provides a system for inhibiting the binding between two cells, one expressing P- or L-selectin on the surface and the other expressing the corresponding ligand. A covalently crosslinked lipid composition is prepared having saccharides and acidic group on separate lipids. The composition is then interposed between the cells so as to inhibit binding. Inhibition can be achieved at an effective oligosaccharide concentration as low as 10.sup.6 fold below that of the free saccharide. Since selectins are involved in recruiting cells to sites of injury, this system can be used to palliate certain inflammatory and immunological conditions.

  15. Inhibition of selectin binding

    DOE Patents [OSTI]

    Nagy, Jon O. (Rodeo, CA); Spevak, Wayne R. (Albany, CA); Dasgupta, Falguni (New Delhi, IN); Bertozzi, Caroline (Albany, CA)

    1999-01-01

    This invention provides compositions for inhibiting the binding between two cells, one expressing P- or L-selectin on the surface and the other expressing the corresponding ligand. A covalently crosslinked lipid composition is prepared having saccharides and acidic group on separate lipids. The composition is then interposed between the cells so as to inhibit binding. Inhibition can be achieved at an effective oligosaccharide concentration as low as 10.sup.6 fold below that of the free saccharide. Since selectins are involved in recruiting cells to sites of injury, these composition scan be used to palliate certain inflammatory and immunological conditions.

  16. Cellulose binding domain proteins

    DOE Patents [OSTI]

    Shoseyov, Oded (Karmey Yosef, IL); Shpiegl, Itai (Rehovot, IL); Goldstein, Marc (Davis, CA); Doi, Roy (Davis, CA)

    1998-01-01

    A cellulose binding domain (CBD) having a high affinity for crystalline cellulose and chitin is disclosed, along with methods for the molecular cloning and recombinant production thereof. Fusion products comprising the CBD and a second protein are likewise described. A wide range of applications are contemplated for both the CBD and the fusion products, including drug delivery, affinity separations, and diagnostic techniques.

  17. Cellulose binding domain proteins

    DOE Patents [OSTI]

    Shoseyov, O.; Shpiegl, I.; Goldstein, M.; Doi, R.

    1998-11-17

    A cellulose binding domain (CBD) having a high affinity for crystalline cellulose and chitin is disclosed, along with methods for the molecular cloning and recombinant production. Fusion products comprising the CBD and a second protein are likewise described. A wide range of applications are contemplated for both the CBD and the fusion products, including drug delivery, affinity separations, and diagnostic techniques. 16 figs.

  18. Proposed Project: Plains & Eastern Clean Line Transmission Line...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Section 1222 of the Energy Policy Act 2005 Proposed Project: Plains & Eastern Clean Line Transmission Line Proposed Project: Plains & Eastern Clean Line Transmission Line On ...

  19. Plains and Eastern Clean Line Transmission Line: Federal Register...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Plains and Eastern Clean Line Transmission Line: Federal Register Notice, Volume 80, No. 81 - April 28, 2015 Plains and Eastern Clean Line Transmission Line: Federal Register ...

  20. Anonymous Help Line

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ombuds Program » Anonymous Help Line Ombuds Anonymous Help Line Committed to the fair and equitable treatment of all employees, contractors, and persons doing business with the Laboratory. Contact Ombuds Office (505)665-2837 Email Help Line (505) 667-9370 Fax (505) 667-3119 Map & Hours Anonymous Help Line: (505) 667-9370 As an option to visiting the Ombuds office, we provide service through a telephone Help Line and email. The telephone Help Line is useful for individuals who have concerns

  1. Plains & Eastern Clean Line HVDC Scheme

    Broader source: Energy.gov (indexed) [DOE]

    Line HVDC Scheme

  2. Plains and Eastern Clean Line Transmission Line: Comment from...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Plains and Eastern Clean Line Transmission Line: Comment from Block Plains and Eastern Clean Line: Arkansas and Oklahoma Comment submitted on updated Part 2 application. PDF icon ...

  3. Series Transmission Line Transformer

    DOE Patents [OSTI]

    Buckles, Robert A.; Booth, Rex; Yen, Boris T.

    2004-06-29

    A series transmission line transformer is set forth which includes two or more of impedance matched sets of at least two transmissions lines such as shielded cables, connected in parallel at one end ans series at the other in a cascading fashion. The cables are wound about a magnetic core. The series transmission line transformer (STLT) which can provide for higher impedance ratios and bandwidths, which is scalable, and which is of simpler design and construction.

  4. Maersk Line Equipment guide

    National Nuclear Security Administration (NNSA)

    Maersk Line Equipment guide We create opportunities in global commerce With approximately 2 million container units of every size and type, Maersk Line has a container to suit your needs. Our fleet of modern Maersk Line containers has an average age of less than six years and a stringent maintenance programme ensures they are always ready for service. Each container in our fleet meets the Convention for Safe Containers (CSC) standards. Size feet / inches Door openings* mm feet / inches Internal

  5. Transmission Business Line

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Transmission Business Line Non-Federal Financing of Transmission Projects - March 2004 Critical paths on the Northwest transmission grid are congested and the system is near or at...

  6. Plains and Eastern Clean Line Transmission Line: Comment from Chantel |

    Energy Savers [EERE]

    Department of Energy from Chantel Plains and Eastern Clean Line Transmission Line: Comment from Chantel Comment submitted on updated Part 2 application. PDF icon Comment from Chantel 06-09-15.pdf More Documents & Publications Plains and Eastern Clean Line Transmission Line: Comment from Mr. Garrett Plains and Eastern Clean Line Transmission Line: Comment from Ms. Rutherford Plains and Eastern Clean Line Transmission Line: Comment from Ms. Campbell

  7. Plains and Eastern Clean Line Transmission Line: Comment from Marshall

    Energy Savers [EERE]

    Hughes | Department of Energy from Marshall Hughes Plains and Eastern Clean Line Transmission Line: Comment from Marshall Hughes Comment submitted on updated Part 2 application. PDF icon Comment from Marshall Hughes 07-10-15.pdf More Documents & Publications Plains and Eastern Clean Line Transmission Line: Comment from Mr. Dyer Plains and Eastern Clean Line Transmission Line: Comment from Sheila Beck Plains and Eastern Clean Line Transmission Line: Comment from Patti McCutchen

  8. Transmission Line Security Monitor

    ScienceCinema (OSTI)

    None

    2013-05-28

    The Transmission Line Security Monitor is a multi-sensor monitor that mounts directly on high-voltage transmission lines to detect, characterize and communicate terrorist activity, human tampering and threatening conditions around support towers. For more information about INL's critical infrastructure protection research, visit http://www.facebook.com/idahonationallaboratory.

  9. Transmission Line Security Monitor

    SciTech Connect (OSTI)

    2011-01-01

    The Transmission Line Security Monitor is a multi-sensor monitor that mounts directly on high-voltage transmission lines to detect, characterize and communicate terrorist activity, human tampering and threatening conditions around support towers. For more information about INL's critical infrastructure protection research, visit http://www.facebook.com/idahonationallaboratory.

  10. Summary

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    870 nm AOD of 0.5. The two competitive aerosol models fitting within 0.01 error margin (grey area) are shown by dashed and dash-dotted lines. Right: Same as left but with adding...

  11. Coherent soliton communication lines

    SciTech Connect (OSTI)

    Yushko, O. V. Redyuk, A. A.; Fedoruk, M. P.; Turitsyn, S. K.

    2014-11-15

    The data transmission in coherent fiber-optical communication lines using solitons with a variable phase is studied. It is shown that nonlinear coherent structures (solitons) can be applied for effective signal transmission over a long distance using amplitude and optical-phase keying of information. The optimum ratio of the pulse width to the bit slot at which the spectral efficiency (transmitted bits per second and hertz) is maximal is determined. It is shown that soliton fiber-optical communication lines can ensure data transmission at a higher spectral efficiency as compared to traditional communication lines and at a high signal-to-noise ratio.

  12. Flow line sampler

    DOE Patents [OSTI]

    Nicholls, Colin I. (San Antonio, TX)

    1992-07-14

    An on-line product sampling apparatus and method for measuring product samples from a product stream (12) in a flow line (14) having a sampling aperture (11), includes a sampling tube (18) for containing product samples removed from flow line (14). A piston (22) removes product samples from the product stream (12) through the sampling aperture (11) and returns samples to product stream (12). A sensor (20) communicates with sample tube (18), and senses physical properties of samples while the samples are within sample tube (18). In one embodiment, sensor (20) comprises a hydrogen transient nuclear magnetic resonance sensor for measuring physical properties of hydrogen molecules.

  13. Line Extension Alternatives

    Broader source: Energy.gov [DOE]

    The Public Service Board (PSB) of Vermont developed rules regarding utility line extension requests. While the majority of the rules focus on the procedure followed (and associated fees) for the...

  14. Power line detection system

    DOE Patents [OSTI]

    Latorre, Victor R. (Tracy, CA); Watwood, Donald B. (Tracy, CA)

    1994-01-01

    A short-range, radio frequency (RF) transmitting-receiving system that provides both visual and audio warnings to the pilot of a helicopter or light aircraft of an up-coming power transmission line complex. Small, milliwatt-level narrowband transmitters, powered by the transmission line itself, are installed on top of selected transmission line support towers or within existing warning balls, and provide a continuous RF signal to approaching aircraft. The on-board receiver can be either a separate unit or a portion of the existing avionics, and can also share an existing antenna with another airborne system. Upon receipt of a warning signal, the receiver will trigger a visual and an audio alarm to alert the pilot to the potential power line hazard.

  15. Power line detection system

    DOE Patents [OSTI]

    Latorre, V.R.; Watwood, D.B.

    1994-09-27

    A short-range, radio frequency (RF) transmitting-receiving system that provides both visual and audio warnings to the pilot of a helicopter or light aircraft of an up-coming power transmission line complex. Small, milliwatt-level narrowband transmitters, powered by the transmission line itself, are installed on top of selected transmission line support towers or within existing warning balls, and provide a continuous RF signal to approaching aircraft. The on-board receiver can be either a separate unit or a portion of the existing avionics, and can also share an existing antenna with another airborne system. Upon receipt of a warning signal, the receiver will trigger a visual and an audio alarm to alert the pilot to the potential power line hazard. 4 figs.

  16. How Dynein Binds to Microtubules

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    how it binds to microtubules and gives some hints into the fascinating question of how communication passes along the stalk from the MTBD to the rest of the motor. Lucky Break...

  17. How Dynein Binds to Microtubules

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    How Dynein Binds to Microtubules How Dynein Binds to Microtubules Print Wednesday, 29 April 2009 00:00 Cytoplasmic dynein is a protein complex responsible for the transport of a large variety of cargoes, from specific RNAs and proteins to whole organelles, in a directional fashion along microtubules that serve as cellular conveyor belts. Consistent with this central role, cytoplasmic dynein is associated with a number of disease-related processes, including the transport of viruses,

  18. Transmission line capital costs

    SciTech Connect (OSTI)

    Hughes, K.R.; Brown, D.R.

    1995-05-01

    The displacement or deferral of conventional AC transmission line installation is a key benefit associated with several technologies being developed with the support of the U.S. Department of Energy`s Office of Energy Management (OEM). Previous benefits assessments conducted within OEM have been based on significantly different assumptions for the average cost per mile of AC transmission line. In response to this uncertainty, an investigation of transmission line capital cost data was initiated. The objective of this study was to develop a database for preparing preliminary estimates of transmission line costs. An extensive search of potential data sources identified databases maintained by the Bonneville Power Administration (BPA) and the Western Area Power Administration (WAPA) as superior sources of transmission line cost data. The BPA and WAPA data were adjusted to a common basis and combined together. The composite database covers voltage levels from 13.8 to 765 W, with cost estimates for a given voltage level varying depending on conductor size, tower material type, tower frame type, and number of circuits. Reported transmission line costs vary significantly, even for a given voltage level. This can usually be explained by variation in the design factors noted above and variation in environmental and land (right-of-way) costs, which are extremely site-specific. Cost estimates prepared from the composite database were compared to cost data collected by the Federal Energy Regulatory Commission (FERC) for investor-owned utilities from across the United States. The comparison was hampered because the only design specifications included with the FERC data were voltage level and line length. Working within this limitation, the FERC data were not found to differ significantly from the composite database. Therefore, the composite database was judged to be a reasonable proxy for estimating national average costs.

  19. Proposed Project: Plains & Eastern Clean Line Transmission Line |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Section 1222 of the Energy Policy Act 2005 » Proposed Project: Plains & Eastern Clean Line Transmission Line Proposed Project: Plains & Eastern Clean Line Transmission Line On June 10, 2010, the Department of Energy published in the Federal Register a Request for Proposals (RFP) for New or Upgraded Transmission Line Projects Under Section 1222 of the Energy Policy Act (EPAct) of 2005. In response, Clean Line Energy Partners, LLC submitted an application for its

  20. Plains and Eastern Clean Line Transmission Line: Comment from...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    from Dr. Contreras Plains and Eastern Clean Line Transmission Line: Comment from Dr. Contreras Comment submitted on updated Part 2 application. PDF icon Comment from Dr. Contreras ...

  1. Plains and Eastern Clean Line Transmission Line: Comment from...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    from Marshall Hughes Plains and Eastern Clean Line Transmission Line: Comment from Marshall Hughes Comment submitted on updated Part 2 application. PDF icon Comment from Marshall ...

  2. Plains and Eastern Clean Line Transmission Line: Comment from...

    Broader source: Energy.gov (indexed) [DOE]

    Comment submitted on updated Part 2 application. Comment from Lewis Cain 6-8-15.pdf More Documents & Publications Plains and Eastern Clean Line Transmission Line: Comment from...

  3. Bonneville upgrades lines

    SciTech Connect (OSTI)

    Not Available

    1993-08-30

    A stretch of Bonneville Power Administration transmission line between Spokane and the Grand Coulee Dam is an 82-mile bottleneck as it is the last 115-kv section of [open quotes]road[close quotes] along a 500-kv transmission [open quotes]highway[close quotes]. Soon the administration will change all that. A number of independent and utility powerplants have been proposed in Idaho, Montana and the Spokane, Wash., area during the 1990s. As the operators will need to move their power, Bonneville is planning to build a $144-million, 500-kv line to upgrade the 115-kv section. The agency is planning to use its existing right-of-way.

  4. ONE NEVADA LINE | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ONE NEVADA LINE ONE NEVADA LINE ONE NEVADA LINE ONE NEVADA LINE ONE NEVADA LINE ONE NEVADA LINE ONE NEVADA LINE ONE NEVADA LINE ONE NEVADA LINE ONE NEVADA LINE ONE NEVADA LINE ONE NEVADA LINE ONE NEVADA LINE ONE NEVADA LINE ONE NEVADA LINE PROJECT SUMMARY In February 2011, the Department of Energy issued a $343 million loan guarantee to finance the One Nevada Transmission Line (ON Line) project, a 235-mile, 500 kV AC transmission line capable of carrying 600 MW of power to the grid running

  5. Synthetic heparin-binding growth factor analogs

    DOE Patents [OSTI]

    Pena, Louis A.; Zamora, Paul; Lin, Xinhua; Glass, John D.

    2007-01-23

    The invention provides synthetic heparin-binding growth factor analogs having at least one peptide chain that binds a heparin-binding growth factor receptor, covalently bound to a hydrophobic linker, which is in turn covalently bound to a non-signaling peptide that includes a heparin-binding domain. The synthetic heparin-binding growth factor analogs are useful as soluble biologics or as surface coatings for medical devices.

  6. Plains and Eastern Clean Line Transmission Line: Federal Register Notice,

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Volume 80, No. 81 - April 28, 2015 | Department of Energy Plains and Eastern Clean Line Transmission Line: Federal Register Notice, Volume 80, No. 81 - April 28, 2015 Plains and Eastern Clean Line Transmission Line: Federal Register Notice, Volume 80, No. 81 - April 28, 2015 The Department of Energy (DOE) requests public comment on the first complete application submitted in response to its June 10, 2010 Request for Proposals for New or Upgraded Transmission Line Projects Under Section 1222

  7. Plains & Eastern Clean Line Transmission Line - Part 2 Application |

    Energy Savers [EERE]

    Department of Energy Transmission Line - Part 2 Application Plains & Eastern Clean Line Transmission Line - Part 2 Application In addition to conducting a NEPA review of the proposed Plains & Eastern Clean Line Transmission Line project, DOE will also conduct due diligence on non-NEPA factors such as the project's technical and financial feasibility and whether the project is in the public interest. DOE will conduct a thorough review that includes making all required statutory

  8. Cellulose binding domain fusion proteins

    DOE Patents [OSTI]

    Shoseyov, O.; Yosef, K.; Shpiegl, I.; Goldstein, M.A.; Doi, R.H.

    1998-02-17

    A cellulose binding domain (CBD) having a high affinity for crystalline cellulose and chitin is disclosed, along with methods for the molecular cloning and recombinant production. Fusion products comprising the CBD and a second protein are likewise described. A wide range of applications are contemplated for both the CBD and the fusion products, including drug delivery, affinity separations, and diagnostic techniques. 16 figs.

  9. Cellulose binding domain fusion proteins

    DOE Patents [OSTI]

    Shoseyov, Oded (Karmey Yosef, IL); Shpiegl, Itai (Rehovot, IL); Goldstein, Marc A. (Davis, CA); Doi, Roy H. (Davis, CA)

    1998-01-01

    A cellulose binding domain (CBD) having a high affinity for crystalline cellulose and chitin is disclosed, along with methods for the molecular cloning and recombinant production thereof. Fusion products comprising the CBD and a second protein are likewise described. A wide range of applications are contemplated for both the CBD and the fusion products, including drug delivery, affinity separations, and diagnostic techniques.

  10. Printed circuit dispersive transmission line

    DOE Patents [OSTI]

    Ikezi, H.; Lin-Liu, Y.R.; DeGrassie, J.S.

    1991-08-27

    A printed circuit dispersive transmission line structure is disclosed comprising an insulator, a ground plane formed on one surface of the insulator, a first transmission line formed on a second surface of the insulator, and a second transmission line also formed on the second surface of the insulator and of longer length than the first transmission line and periodically intersecting the first transmission line. In a preferred embodiment, the transmission line structure exhibits highly dispersive characteristics by designing the length of one of the transmission line between two adjacent periodic intersections to be longer than the other. 5 figures.

  11. Printed circuit dispersive transmission line

    DOE Patents [OSTI]

    Ikezi, Hiroyuki (Rancho Santa Fe, CA); Lin-Liu, Yuh-Ren (San Diego, CA); DeGrassie, John S. (Encinitas, CA)

    1991-01-01

    A printed circuit dispersive transmission line structure is disclosed comprising an insulator, a ground plane formed on one surface of the insulator, a first transmission line formed on a second surface of the insulator, and a second transmission line also formed on the second surface of the insulator and of longer length than the first transmission line and periodically intersecting the first transmission line. In a preferred embodiment, the transmission line structure exhibits highly dispersive characteristics by designing the length of one of the transmission line between two adjacent periodic intersections to be longer than the other.

  12. Drill string transmission line

    DOE Patents [OSTI]

    Hall, David R. (Provo, UT); Hall, Jr., H. Tracy (Provo, UT); Pixton, David S. (Lehi, UT); Bradford, Kline (Orem, UT); Fox, Joe (Spanish Fork, UT)

    2006-03-28

    A transmission line assembly for transmitting information along a downhole tool comprising a pin end, a box end, and a central bore traveling between the pin end and the box end, is disclosed in one embodiment of the invention as including a protective conduit. A transmission line is routed through the protective conduit. The protective conduit is routed through the central bore and the ends of the protective conduit are routed through channels formed in the pin end and box end of the downhole tool. The protective conduit is elastically forced into a spiral or other non-linear path along the interior surface of the central bore by compressing the protective conduit to a length within the downhole tool shorter than the protective conduit.

  13. Spectroscopic Evidence for the Phase Competition between the...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    results, the back-bending of the pseudogap line (dashed line) in the superconducting dome is suggested. The researchers further expanded their study into the doping dependence....

  14. How Dynein Binds to Microtubules

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    How Dynein Binds to Microtubules Print Cytoplasmic dynein is a protein complex responsible for the transport of a large variety of cargoes, from specific RNAs and proteins to whole organelles, in a directional fashion along microtubules that serve as cellular conveyor belts. Consistent with this central role, cytoplasmic dynein is associated with a number of disease-related processes, including the transport of viruses, neurodegeneration, and the mitotic checkpoint malfunctions that lead to

  15. How Dynein Binds to Microtubules

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    How Dynein Binds to Microtubules Print Cytoplasmic dynein is a protein complex responsible for the transport of a large variety of cargoes, from specific RNAs and proteins to whole organelles, in a directional fashion along microtubules that serve as cellular conveyor belts. Consistent with this central role, cytoplasmic dynein is associated with a number of disease-related processes, including the transport of viruses, neurodegeneration, and the mitotic checkpoint malfunctions that lead to

  16. How Dynein Binds to Microtubules

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    How Dynein Binds to Microtubules Print Cytoplasmic dynein is a protein complex responsible for the transport of a large variety of cargoes, from specific RNAs and proteins to whole organelles, in a directional fashion along microtubules that serve as cellular conveyor belts. Consistent with this central role, cytoplasmic dynein is associated with a number of disease-related processes, including the transport of viruses, neurodegeneration, and the mitotic checkpoint malfunctions that lead to

  17. 2005 EIA-821 SURVEY: LINE-BY-LINE REFERENCE GUIDE

    Gasoline and Diesel Fuel Update (EIA)

    FORM EIA-821 ANNUAL FUEL OIL AND KEROSENE SALES REPORT LINE-BY-LINE REFERENCE GUIDE Kerosene Line No. Sold directly to consumers for: 1 Residential Use (Non-Farm): * Backup generator * Home heating and cooking * Personal lawn equipment * EXCLUDE: Apartment buildings and Farmhouses 2 Commercial Use: * Apartment building * Bank * Casino * Church * College/School/Institution * Department/Retail store * Environmental clean-up service * Flushing fuel lines * Forestry service * Golf course *

  18. Climate change: Clinton affirms binding emissions reduction policy

    SciTech Connect (OSTI)

    Fairley, P.

    1996-12-04

    In Australia last month President Clinton called for an international agreement to negotiate {open_quotes}legally binding commitments to fight climate change.{close_quotes} His comments affirmed the line the Administration adopted in July and lent prominence to the effort to bring about a treaty by December 1997. Environmentalists welcomed Clinton`s comments, but industry response is divided. The Global Climate Coalition (Washington), of which CMA is a member, has tried to slow negotiations by questioning the scientific consensus on climate change and suggesting {open_quotes}serious damage to the American economy{close_quotes} could result from emissions reduction.

  19. Erythropoietin binding protein from mammalian serum

    DOE Patents [OSTI]

    Clemons, Gisela K.

    1997-01-01

    Purified mammalian erythropoietin binding-protein is disclosed, and its isolation, identification, characterization, purification, and immunoassay are described. The erythropoietin binding protein can be used for regulation of erythropoiesis by regulating levels and half-life of erythropoietin. A diagnostic kit for determination of level of erythropoietin binding protein is also described.

  20. Erythropoietin binding protein from mammalian serum

    DOE Patents [OSTI]

    Clemons, G.K.

    1997-04-29

    Purified mammalian erythropoietin binding-protein is disclosed, and its isolation, identification, characterization, purification, and immunoassay are described. The erythropoietin binding protein can be used for regulation of erythropoiesis by regulating levels and half-life of erythropoietin. A diagnostic kit for determination of level of erythropoietin binding protein is also described. 11 figs.

  1. Synthetic heparin-binding factor analogs

    DOE Patents [OSTI]

    Pena, Louis A.; Zamora, Paul O.; Lin, Xinhua; Glass, John D.

    2010-04-20

    The invention provides synthetic heparin-binding growth factor analogs having at least one peptide chain, and preferably two peptide chains branched from a dipeptide branch moiety composed of two trifunctional amino acid residues, which peptide chain or chains bind a heparin-binding growth factor receptor and are covalently bound to a non-signaling peptide that includes a heparin-binding domain, preferably by a linker, which may be a hydrophobic linker. The synthetic heparin-binding growth factor analogs are useful as pharmaceutical agents, soluble biologics or as surface coatings for medical devices.

  2. Bipolar pulse forming line

    DOE Patents [OSTI]

    Rhodes, Mark A. (Pleasanton, CA)

    2008-10-21

    A bipolar pulse forming transmission line module for linear induction accelerators having first, second, third, fourth, and fifth planar conductors which form an interleaved stack with dielectric layers between the conductors. Each conductor has a first end, and a second end adjacent an acceleration axis. The first and second planar conductors are connected to each other at the second ends, the fourth and fifth planar conductors are connected to each other at the second ends, and the first and fifth planar conductors are connected to each other at the first ends via a shorting plate adjacent the first ends. The third planar conductor is electrically connectable to a high voltage source, and an internal switch functions to short a high voltage from the first end of the third planar conductor to the first end of the fourth planar conductor to produce a bipolar pulse at the acceleration axis with a zero net time integral. Improved access to the switch is enabled by an aperture through the shorting plate and the proximity of the aperture to the switch.

  3. EC Transmission Line Materials

    SciTech Connect (OSTI)

    Bigelow, Tim S

    2012-05-01

    The purpose of this document is to identify materials acceptable for use in the US ITER Project Office (USIPO)-supplied components for the ITER Electron cyclotron Heating and Current Drive (ECH&CD) transmission lines (TL), PBS-52. The source of material property information for design analysis shall be either the applicable structural code or the ITER Material Properties Handbook. In the case of conflict, the ITER Material Properties Handbook shall take precedence. Materials selection, and use, shall follow the guidelines established in the Materials Assessment Report (MAR). Materials exposed to vacuum shall conform to the ITER Vacuum Handbook. [Ref. 2] Commercial materials shall conform to the applicable standard (e.g., ASTM, JIS, DIN) for the definition of their grade, physical, chemical and electrical properties and related testing. All materials for which a suitable certification from the supplier is not available shall be tested to determine the relevant properties, as part of the procurement. A complete traceability of all the materials including welding materials shall be provided. Halogenated materials (example: insulating materials) shall be forbidden in areas served by the detritiation systems. Exceptions must be approved by the Tritium System and Safety Section Responsible Officers.

  4. NETL LINES OF DEMARCATION 09282012

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    LINES OF DEMARCATION September 28, 2012 Contact: Site Operations Division or ESS&H Division with Questions The oversight, upkeep, and segregation of NETL infrastructure components, including lines of demarcation between ORD and OIO, will be as follows: 1. Common utility headers including gas compression systems that enter or exit the site will be maintained by the Office of Institutional Operations (OIO). A line of responsibility demarcation between OIO and ORD will be established at the

  5. Emergency pipe line repair connects subsea pipe lines

    SciTech Connect (OSTI)

    Lerique, M.P.; Thiberge, P. ); Wright, N. )

    1990-11-01

    Emergency repair of any subsea line pipe must form a high-integrity, metal-to-metal seal. This paper presents a remote, diverless repair system that utilizes master flanges, a connector and a spool piece to repair line pipe in deep offshore waters.

  6. Binding Organic Liquids - Energy Innovation Portal

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    More Like This Return to Search Binding Organic Liquids Pacific Northwest National Laboratory Contact PNNL About This Technology Technology Marketing Summary Researchers at...

  7. Electric Transmission Line Siting Compact

    Office of Environmental Management (EM)

    Electric Transmission Line Siting Compact 1 ______________________________________________________________________________ 2 ARTICLE I 3 PURPOSE 4 5 Siting electric transmission lines across state borders and federal lands is an issue for states, the 6 federal government, transmission utilities, consumers, environmentalists, and other stakeholders. 7 The current, multi-year application review process by separate and equal jurisdictions constitutes 8 a sometimes inefficient and redundant process

  8. Nicotinamide mononucleotide adenylyltransferase displays alternate binding

    Office of Scientific and Technical Information (OSTI)

    modes for nicotinamide nucleotides (Journal Article) | SciTech Connect Nicotinamide mononucleotide adenylyltransferase displays alternate binding modes for nicotinamide nucleotides Citation Details In-Document Search Title: Nicotinamide mononucleotide adenylyltransferase displays alternate binding modes for nicotinamide nucleotides Authors: Pfoh, Roland ; Pai, Emil F. ; Saridakis, Vivian [1] ; Toronto) [2] + Show Author Affiliations (York) ( Publication Date: 2015-11-23 OSTI Identifier:

  9. Annual Energy Outlook 2013 Early Release Reference Case

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    price per barrel (real 2010 dollars) Sources: U.S. Energy Information Administration, Thomson Reuters Prices shown are quarterly averages: dashed lines are EIA projections...

  10. X:\\ARM_19~1\\P335-353.WPD

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    forcing as computed with the doubling and adding model (see text). Dashed lines: heuristic "models" explaining the main differences between the direct and indirect forcing...

  11. Side-on Cu-Nitrosyl Coordination by Nitrite Reductase

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    (solid), as well as H bonds and other electrostatic interactions (dashed), are shown as grey lines of the type indicated. Carbons (black), oxygens (red), nitrogens (blue), type 2...

  12. Research Highlight

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    six different absorption (permittivity) models at 0 C (top) and -25 C (bottom). The grey dashed line shows water vapor absorption for comparison (multiplied by a factor of...

  13. Electrical transmission line diametrical retainer

    DOE Patents [OSTI]

    Hall, David R.; Hall, Jr., H. Tracy; Pixton, David; Dahlgren, Scott; Sneddon, Cameron; Briscoe, Michael; Fox, Joe

    2004-12-14

    The invention is a mechanism for retaining an electrical transmission line. In one embodiment of the invention it is a system for retaining an electrical transmission line within down hole components. In accordance with one aspect of the invention, the system includes a plurality of downhole components, such as sections of pipe in a drill string. The system also includes a coaxial cable running between the first and second end of a drill pipe, the coaxial cable having a conductive tube and a conductive core within it. The invention allows the electrical transmission line to with stand the tension and compression of drill pipe during routine drilling cycles.

  14. Pulse shaping with transmission lines

    DOE Patents [OSTI]

    Wilcox, Russell B.

    1987-01-01

    A method and apparatus for forming shaped voltage pulses uses passive reflection from a transmission line with nonuniform impedance. The impedance of the reflecting line varies with length in accordance with the desired pulse shape. A high voltage input pulse is transmitted to the reflecting line. A reflected pulse is produced having the desired shape and is transmitted by pulse removal means to a load. Light activated photoconductive switches made of silicon can be utilized. The pulse shaper can be used to drive a Pockels cell to produce shaped optical pulses.

  15. Pulse shaping with transmission lines

    DOE Patents [OSTI]

    Wilcox, R.B.

    1985-08-15

    A method and apparatus for forming shaped voltage pulses uses passive reflection from a transmission line with nonuniform impedance. The impedance of the reflecting line varies with length in accordance with the desired pulse shape. A high voltage input pulse is transmitted to the reflecting line. A reflected pulse is produced having the desired shape and is transmitted by pulse removal means to a load. Light activated photoconductive switches made of silicon can be utilized. The pulse shaper can be used to drive a Pockels cell to produce shaped optical pulses.

  16. SSRL Beam Lines Map | Stanford Synchrotron Radiation Lightsource

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    SSRL Beam Lines Map Beam Line by Number | Beam Line by Techniques | Photon Source Parameters

  17. Improved flow cytometer measurement of binding assays

    DOE Patents [OSTI]

    Saunders, G.C.

    1984-05-30

    The invention relates to a method of measuring binding assays carried out with different size particles wherein the binding assay sample is run through a flow cytometer without separating the sample from the marking agent. The amount of a binding reactant present in a sample is determined by providing particles with a coating of binder and also a known quantity of smaller particles with a coating of binder reactant. The binding reactant is the same as the binding reactant present in the sample. The smaller particles also contain a fluorescent chemical. The particles are combined with the sample and the binding reaction is allowed to occur for a set length of time followed by combining the smaller particles with the mixture of the particles and the sample produced and allowing the binding reactions to proceed to equilibrium. The fluorescence and light scatter of the combined mixture is then measured as the combined mixture passes through a flow cytometer equipped with a laser to bring about fluorescence, and the number and strength of fluorescent events are compared. A similar method is also provided for determining the amount of antigen present in the sample by providing spheres with an antibody coating and some smaller spheres with an antigen coating. (LEW)

  18. Fin-line horn antenna

    DOE Patents [OSTI]

    Reindel, John (San Diego, CA)

    1990-01-01

    A fin line circuit card containing a fin line slot feeds a dipole antenna ich extends a quarterwave outside the waveguide and provides an energy beam focal point at or near the open end of the waveguide. The dipole antenna thus maintains a wide and nearly constant beamwidth, low VSWR and a circular symmetric radiation pattern for use in electronic warfare direction finding and surveillance applications.

  19. Observation Wells At Fenton Hill HDR Geothermal Area (Dash, Et...

    Open Energy Info (EERE)

    Dennis, Donald S. Dreesen, Leigh S. House, Hugh D. Murphy, Bruce A. Robinson, Morton C. Smith (1987) The US Hot Dry Rock Project Additional References Retrieved from "http:...

  20. Letter from Deputy Secretary Poneman to Clean Line Energy Regarding...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Line: Comment from Ms. Schroeder Plains and Eastern Clean Line Transmission Line: Comment from Mr. Zuniga Plains and Eastern Clean Line Transmission Line: Comment from Mr. Cain...

  1. Localized states of a semi-infinite zigzag graphene sheet with impurity lines

    SciTech Connect (OSTI)

    Cunha, A. M. C.; Ahmed, Maher Z.; Cottam, M. G.; Filho, R. N. Costa

    2014-07-07

    The localized states of a semi-infinite zigzag graphene sheet are studied using a tight-binding model that allows for the inclusion of either one or two lines of impurities. These impurity lines of atoms are placed in rows labeled as n (n=1, 2, 3, …), where n=1 is the free edge. The localized defect modes associated with these impurities are studied analytically and numerically within a tridiagonal matrix formalism. For one impurity line, the modes are analyzed according to the position of that line on the sheet, whereas the modes for two impurities are studied also according to their separation and their positions relative to the edge. When an impurity line is located at the edge (n=1), it is found that the edge states are modified. When the impurities are positioned away from an edge (n>1), additional localized modes are found to occur that may be relatively flat in their dispersion.

  2. Hardware device binding and mutual authentication

    DOE Patents [OSTI]

    Hamlet, Jason R; Pierson, Lyndon G

    2014-03-04

    Detection and deterrence of device tampering and subversion by substitution may be achieved by including a cryptographic unit within a computing device for binding multiple hardware devices and mutually authenticating the devices. The cryptographic unit includes a physically unclonable function ("PUF") circuit disposed in or on the hardware device, which generates a binding PUF value. The cryptographic unit uses the binding PUF value during an enrollment phase and subsequent authentication phases. During a subsequent authentication phase, the cryptographic unit uses the binding PUF values of the multiple hardware devices to generate a challenge to send to the other device, and to verify a challenge received from the other device to mutually authenticate the hardware devices.

  3. Sequestering Uranium from Seawater: Binding Strength and Modes...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Sequestering Uranium from Seawater: Binding Strength and Modes of Uranyl Complexes with Glutarimidedioxime Sequestering Uranium from Seawater: Binding Strength and Modes of Uranyl...

  4. Fragment-Based Exploration of Binding Site Flexibility in Mycobacteriu...

    Office of Scientific and Technical Information (OSTI)

    Fragment-Based Exploration of Binding Site Flexibility in Mycobacterium tuberculosis BioA Citation Details In-Document Search Title: Fragment-Based Exploration of Binding Site ...

  5. ONE NEVADA LINE | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ONE NEVADA LINE ONE NEVADA LINE PDF icon DOE-LPO_Project-Posters_STOR-TRSM_One-Nevada-Line.pdf More Documents & Publications STEPHENTOWN SPINDLE CX-011989: Categorical Exclusion Determination CRESCENT DUNES

  6. RNA binding protein and binding site useful for expression of recombinant molecules

    DOE Patents [OSTI]

    Mayfield, Stephen

    2000-01-01

    The present invention relates to a gene expression system in eukaryotic and prokaryotic cells, preferably plant cells and intact plants. In particular, the invention relates to an expression system having a RB47 binding site upstream of a translation initiation site for regulation of translation mediated by binding of RB47 protein, a member of the poly(A) binding protein family. Regulation is further effected by RB60, a protein disulfide isomerase. The expression system is capable of functioning in the nuclear/cytoplasm of cells and in the chloroplast of plants. Translation regulation of a desired molecule is enhanced approximately 100 fold over that obtained without RB47 binding site activation.

  7. RNA binding protein and binding site useful for expression of recombinant molecules

    DOE Patents [OSTI]

    Mayfield, Stephen P.

    2006-10-17

    The present invention relates to a gene expression system in eukaryotic and prokaryotic cells, preferably plant cells and intact plants. In particular, the invention relates to an expression system having a RB47 binding site upstream of a translation initiation site for regulation of translation mediated by binding of RB47 protein, a member of the poly(A) binding protein family. Regulation is further effected by RB60, a protein disulfide isomerase. The expression system is capable of functioning in the nuclear/cytoplasm of cells and in the chloroplast of plants. Translation regulation of a desired molecule is enhanced approximately 100 fold over that obtained without RB47 binding site activation.

  8. Plains and Eastern Clean Line Transmission Line: Comment from Block Plains

    Energy Savers [EERE]

    and Eastern Clean Line: Arkansas and Oklahoma | Department of Energy from Block Plains and Eastern Clean Line: Arkansas and Oklahoma Plains and Eastern Clean Line Transmission Line: Comment from Block Plains and Eastern Clean Line: Arkansas and Oklahoma Comment submitted on updated Part 2 application. PDF icon Comment from Block Plains & Eastern Clean Line Arkansas and Oklahoma 06-08-15.pdf More Documents & Publications Plains and Eastern Clean Line Transmission Line: Comment from

  9. Plains and Eastern Clean Line Transmission Line: Comment from Dr. Contreras

    Energy Savers [EERE]

    | Department of Energy from Dr. Contreras Plains and Eastern Clean Line Transmission Line: Comment from Dr. Contreras Comment submitted on updated Part 2 application. PDF icon Comment from Dr. Contreras 6-10-15.pdf More Documents & Publications Plains and Eastern Clean Line Transmission Line: Comment from Dr. Contreras Plains and Eastern Clean Line Transmission Line: Comment from Dr. Contreras Plains and Eastern Clean Line Transmission Line: Comment from Dr. Contreras

  10. Plains and Eastern Clean Line Transmission Line: Comment from Ms. Callahan

    Energy Savers [EERE]

    | Department of Energy Ms. Callahan Plains and Eastern Clean Line Transmission Line: Comment from Ms. Callahan Comment submitted on updated Part 2 application. PDF icon Comment from Ms. Callahan 07-13-15.pdf More Documents & Publications Plains and Eastern Clean Line Transmission Line: Comment from Cynthia Blansett (COE) Plains and Eastern Clean Line Transmission Line: Comment from Dr. Contreras Plains and Eastern Clean Line Transmission Line: Comment from Simon Mahan (SWEA)

  11. Coiled transmission line pulse generators

    DOE Patents [OSTI]

    McDonald, Kenneth Fox (Columbia, MO)

    2010-11-09

    Methods and apparatus are provided for fabricating and constructing solid dielectric "Coiled Transmission Line" pulse generators in radial or axial coiled geometries. The pour and cure fabrication process enables a wide variety of geometries and form factors. The volume between the conductors is filled with liquid blends of monomers, polymers, oligomers, and/or cross-linkers and dielectric powders; and then cured to form high field strength and high dielectric constant solid dielectric transmission lines that intrinsically produce ideal rectangular high voltage pulses when charged and switched into matched impedance loads. Voltage levels may be increased by Marx and/or Blumlein principles incorporating spark gap or, preferentially, solid state switches (such as optically triggered thyristors) which produce reliable, high repetition rate operation. Moreover, these Marxed pulse generators can be DC charged and do not require additional pulse forming circuitry, pulse forming lines, transformers, or an a high voltage spark gap output switch. The apparatus accommodates a wide range of voltages, impedances, pulse durations, pulse repetition rates, and duty cycles. The resulting mobile or flight platform friendly cylindrical geometric configuration is much more compact, light-weight, and robust than conventional linear geometries, or pulse generators constructed from conventional components. Installing additional circuitry may accommodate optional pulse shape improvements. The Coiled Transmission Lines can also be connected in parallel to decrease the impedance, or in series to increase the pulse length.

  12. User Experiment Time-Line

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    User Experiment Time-Line Event Target Call for proposal 2 months before proposals are due PAC Proposals Due 7 weeks before PAC meeting TAC Report for PAC Proposals (includes Independent Technical Review) 4 weeks after proposals are due PAC Report Published 1-2 months after PAC

  13. Plains and Eastern Clean Line Transmission Line: Comment from Downwind, LLC

    Broader source: Energy.gov (indexed) [DOE]

    | Department of Energy Downwind, LLC 6-11-15.pdf More Documents & Publications Plains and Eastern Clean Line Transmission Line: Comment from Downwind, LLC Plains and Eastern Clean Line Transmission Line: Comment from Sheila Beck Plains and Eastern Clean Line Transmission Line: Comment from Crystal Yarbrough

  14. Plains and Eastern Clean Line Transmission Line: Comment from Mr. Eubanks

    Broader source: Energy.gov (indexed) [DOE]

    (OAG) | Department of Energy OAG 07-13-15.pdf More Documents & Publications Plains and Eastern Clean Line Transmission Line: Comment from Mr. Dyer Plains and Eastern Clean Line Transmission Line: Comment from Downwind, LLC Plains and Eastern Clean Line Transmission Line: Comment from Ms. Alinda Baker

  15. Plains and Eastern Clean Line Transmission Line: Comment from Mr. Zuniga |

    Broader source: Energy.gov (indexed) [DOE]

    Department of Energy Juan Zuniga 6-9-15.pdf More Documents & Publications Plains and Eastern Clean Line Transmission Line: Comment from Change.org Plains and Eastern Clean Line Transmission Line: Comment from Mr. Leftwich Plains and Eastern Clean Line Transmission Line: Comment from Leslie Mahoney

  16. Plains and Eastern Clean Line Transmission Line: Comment from Ms. Schroeder

    Broader source: Energy.gov (indexed) [DOE]

    | Department of Energy Schroeder 6-08-15.pdf More Documents & Publications Plains and Eastern Clean Line Transmission Line: Comment from Ms. Shaw Plains and Eastern Clean Line Transmission Line: Comment from Mr. Burningham Plains and Eastern Clean Line Transmission Line: Comment from Fallon Sanford

  17. Exciton binding energy in semiconductor quantum dots

    SciTech Connect (OSTI)

    Pokutnii, S. I.

    2010-04-15

    In the adiabatic approximation in the context of the modified effective mass approach, in which the reduced exciton effective mass {mu} = {mu}(a) is a function of the radius a of the semiconductor quantum dot, an expression for the exciton binding energy E{sub ex}(a) in the quantum dot is derived. It is found that, in the CdSe and CdS quantum dots with the radii a comparable to the Bohr exciton radii a{sub ex}, the exciton binding energy E{sub ex}(a) is substantially (respectively, 7.4 and 4.5 times) higher than the exciton binding energy in the CdSe and CdS single crystals.

  18. In-line thermoelectric module

    DOE Patents [OSTI]

    Pento, Robert (Algonquin, IL); Marks, James E. (Glenville, NY); Staffanson, Clifford D. (S. Glens Falls, NY)

    2000-01-01

    A thermoelectric module with a plurality of electricity generating units each having a first end and a second end, the units being arranged first end to second end along an in-line axis. Each unit includes first and second elements each made of a thermoelectric material, an electrically conductive hot member arranged to heat one side of the first element, and an electrically conductive cold member arranged to cool another side of the first element and to cool one side of the second element. The hot member, the first element, the cold member and the second element are supported in a fixture, are electrically connected respectively to provide an electricity generating unit, and are arranged respectively in positions along the in-line axis. The individual components of each generating unit and the respective generating units are clamped in their in-line positions by a loading bolt at one end of the fixture and a stop wall at the other end of the fixture. The hot members may have a T-shape and the cold members an hourglass shape to facilitate heat transfer. The direction of heat transfer through the hot members may be perpendicular to the direction of heat transfer through the cold members, and both of these heat transfer directions may be perpendicular to the direction of current flow through the module.

  19. Stabilized sulfur binding using activated fillers

    DOE Patents [OSTI]

    Kalb, Paul D.; Vagin, Vyacheslav P.; Vagin, Sergey P.

    2015-07-21

    A method of making a stable, sulfur binding composite comprising impregnating a solid aggregate with an organic modifier comprising unsaturated hydrocarbons with at least one double or triple covalent bond between adjacent carbon atoms to create a modifier-impregnated aggregate; heating and drying the modifier-impregnated aggregate to activate the surface of the modifier-impregnated aggregate for reaction with sulfur.

  20. Nucleic acids encoding a cellulose binding domain

    DOE Patents [OSTI]

    Shoseyov, O.; Shpiegl, I.; Goldstein, M.A.; Doi, R.H.

    1996-03-05

    A cellulose binding domain (CBD) having a high affinity for crystalline cellulose and chitin is disclosed, along with methods for the molecular cloning and recombinant production. Fusion products comprising the CBD and a second protein are likewise described. A wide range of applications are contemplated for both the CBD and the fusion products, including drug delivery, affinity separations, and diagnostic techniques. 15 figs.

  1. Nucleic acids encoding a cellulose binding domain

    DOE Patents [OSTI]

    Shoseyov, Oded (Karmey Yosef, IL); Shpiegl, Itai (Rehovot, IL); Goldstein, Marc A. (Davis, CA); Doi, Roy H. (Davis, CA)

    1996-01-01

    A cellulose binding domain (CBD) having a high affinity for crystalline cellulose and chitin is disclosed, along with methods for the molecular cloning and recombinant production thereof. Fusion products comprising the CBD and a second protein are likewise described. A wide range of applications are contemplated for both the CBD and the fusion products, including drug delivery, affinity separations, and diagnostic techniques.

  2. X-ray line polarization spectroscopy of Li-like satellite line...

    Office of Scientific and Technical Information (OSTI)

    Journal Article: X-ray line polarization spectroscopy of Li-like satellite line spectra Citation Details In-Document Search Title: X-ray line polarization spectroscopy of Li-like...

  3. Plains and Eastern Clean Line Transmission Line: Comment from Save The

    Energy Savers [EERE]

    Ozarks | Department of Energy Save The Ozarks Plains and Eastern Clean Line Transmission Line: Comment from Save The Ozarks Comment submitted on updated Part 2 application. PDF icon Comment by Save the Ozarks 07-13-15.pdf More Documents & Publications Plains & Eastern Clean Line Transmission Line - Part 2 Application Plains & Eastern Clean Line Project Proposal for New or Upgraded Transmission Line Projects Under Section 1222 of the Energy Policy Act of 2005 Plains and Eastern

  4. CXD 4601, Line Yard Fence Project (4601)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Line Yard Fence Project (4601) Y-12 Site Office Oak Ridge, Anderson County, Tennessee The proposed action is to install fencing around the Old Line Yard to provide protected...

  5. Capillary electrophoresis-fluorescence line narrowing system (CE-FLNS) for on-line structural characterization

    DOE Patents [OSTI]

    Jankowiak, R.J.; Small, G.J.; Shields, P.A.

    1999-04-27

    Capillary electrophoresis (CE) is interfaced with low temperature fluorescence line-narrowing (FLN) spectroscopy for on-line structural characterization of separated molecular analytes. 21 figs.

  6. Capillary electrophoresis-fluorescence line narrowing system (CE-FLNS) for on-line structural characterization

    DOE Patents [OSTI]

    Jankowiak, Ryszard J. (Ames, IA); Small, Gerald J. (Ames, IA); Shields, Peter A. (Reading, MA)

    1999-04-27

    Capillary electrophoresis (CE) is interfaced with low temperature fluorescence line-narrowing (FLN) spectroscopy for on-line structural characterization of separated molecular analytes.

  7. O.A.R. 734-055 - Pole Lines, Buried Cables, Pipe lines, Signs...

    Open Energy Info (EERE)

    rules outline the requirements for location, installation, construction, maintenance and use of pole lines, buried cables, pipe lines, signs miscellaneous operations...

  8. Patent: In-line stirling energy system | DOEpatents

    Office of Scientific and Technical Information (OSTI)

    In-line stirling energy system Citation Details Title: In-line stirling energy system

  9. MMW Drilling & Lining | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    MMW Drilling & Lining MMW Drilling & Lining MMW Drilling & Lining presentation at the April 2013 peer review meeting held in Denver, Colorado. PDF icon mmw_drilling_peer2013.pdf More Documents & Publications Auto Indexer for Percussive Hammers CX-007410: Categorical Exclusion Determination Microhole Arrays / FLASH

  10. X-ray crystallographic analysis of adipocyte fatty acid binding...

    Office of Scientific and Technical Information (OSTI)

    binding protein (aP2) modified with 4-hydroxy-2-nonenal Citation Details In-Document Search Title: X-ray crystallographic analysis of adipocyte fatty acid binding protein (aP2) ...

  11. Superconducting transmission line particle detector

    DOE Patents [OSTI]

    Gray, Kenneth E. (Naperville, IL)

    1989-01-01

    A microvertex particle detector for use in a high energy physic collider including a plurality of parallel superconducting thin film strips separated from a superconducting ground plane by an insulating layer to form a plurality of superconducting waveguides. The microvertex particle detector indicates passage of a charged subatomic particle by measuring a voltage pulse measured across a superconducting waveguide caused by the transition of the superconducting thin film strip from a superconducting to a non-superconducting state in response to the passage of a charged particle. A plurality of superconducting thin film strips in two orthogonal planes plus the slow electromagnetic wave propogating in a superconducting transmission line are used to resolve N.sup.2 ambiguity of charged particle events.

  12. Unsplit bipolar pulse forming line

    DOE Patents [OSTI]

    Rhodes, Mark A. (Pleasanton, CA)

    2011-05-24

    A bipolar pulse forming transmission line module and system for linear induction accelerators having first, second, third, and fourth planar conductors which form a sequentially arranged interleaved stack having opposing first and second ends, with dielectric layers between the conductors. The first and second planar conductors are connected to each other at the first end, and the first and fourth planar conductors are connected to each other at the second end via a shorting plate. The third planar conductor is electrically connectable to a high voltage source, and an internal switch functions to short at the first end a high voltage from the third planar conductor to the fourth planar conductor to produce a bipolar pulse at the acceleration axis with a zero net time integral. Improved access to the switch is enabled by an aperture through the shorting plate and the proximity of the aperture to the switch.

  13. Superconducting transmission line particle detector

    DOE Patents [OSTI]

    Gray, K.E.

    1988-07-28

    A microvertex particle detector for use in a high energy physic collider including a plurality of parallel superconducting thin film strips separated from a superconducting ground plane by an insulating layer to form a plurality of superconducting waveguides. The microvertex particle detector indicates passage of a charged subatomic particle by measuring a voltage pulse measured across a superconducting waveguide caused by the transition of the superconducting thin film strip from a superconducting to a non- superconducting state in response to the passage of a charged particle. A plurality of superconducting thin film strips in two orthogonal planes plus the slow electromagnetic wave propagating in a superconducting transmission line are used to resolve N/sup 2/ ambiguity of charged particle events. 6 figs.

  14. Magnetically insulated transmission line oscillator

    DOE Patents [OSTI]

    Bacon, Larry D. (Albuquerque, NM); Ballard, William P. (Albuquerque, NM); Clark, M. Collins (Albuquerque, NM); Marder, Barry M. (Albuquerque, NM)

    1988-01-01

    A magnetically insulated transmission line oscillator employs self-generated magnetic fields to generate microwave energy. An anode of the oscillator includes slow-wave structures which are formed of a plurality of thin conductive vanes defining cavities therebetween, and a gap is formed between the anode and a cathode of the oscillator. In response to a pulsed voltage applied to the anode and cathode, self-generated magnetic fields arfe produced in a cross-field orientation with respect to the orientation of the electric field between the anode and the cathode. The cross-field magnetic fields insulate the flow of electrons in the gap and confine the flow of electrons within the gap.

  15. Magnetically insulated transmission line oscillator

    DOE Patents [OSTI]

    Bacon, L.D.; Ballard, W.P.; Clark, M.C.; Marder, B.M.

    1987-05-19

    A magnetically insulated transmission line oscillator employs self-generated magnetic fields to generate microwave energy. An anode of the oscillator includes slow-wave structures which are formed of a plurality of thin conductive vanes defining cavities therebetween, and a gap is formed between the anode and a cathode of the oscillator. In response to a pulsed voltage applied to the anode and cathode, self-generated magnetic fields are produced in a cross-field orientation with respect to the orientation of the electric field between the anode and the cathode. The cross-field magnetic fields insulate the flow of electrons in the gap and confine the flow of electrons within the gap. 11 figs.

  16. Multidomain Carbohydrate-binding Proteins Involved in Bacteroides...

    Office of Scientific and Technical Information (OSTI)

    Title: Multidomain Carbohydrate-binding Proteins Involved in Bacteroides thetaiotaomicron Starch Metabolism Authors: Cameron, Elizabeth A. ; Maynard, Mallory A. ; Smith, ...

  17. Dual chain synthetic heparin-binding growth factor analogs

    DOE Patents [OSTI]

    Zamora, Paul O.; Pena, Louis A.; Lin, Xinhua

    2009-10-06

    The invention provides synthetic heparin-binding growth factor analogs having two peptide chains each branched from a branch moiety, such as trifunctional amino acid residues, the branch moieties separated by a first linker of from 3 to about 20 backbone atoms, which peptide chains bind a heparin-binding growth factor receptor and are covalently bound to a non-signaling peptide that includes a heparin-binding domain, preferably by a second linker, which may be a hydrophobic second linker. The synthetic heparin-binding growth factor analogs are useful as pharmaceutical agents, soluble biologics or as surface coatings for medical devices.

  18. Dual chain synthetic heparin-binding growth factor analogs

    DOE Patents [OSTI]

    Zamora, Paul O.; Pena, Louis A.; Lin, Xinhua

    2012-04-24

    The invention provides synthetic heparin-binding growth factor analogs having two peptide chains each branched from a branch moiety, such as trifunctional amino acid residues, the branch moieties separated by a first linker of from 3 to about 20 backbone atoms, which peptide chains bind a heparin-binding growth factor receptor and are covalently bound to a non-signaling peptide that includes a heparin-binding domain, preferably by a second linker, which may be a hydrophobic second linker. The synthetic heparin-binding growth factor analogs are useful as pharmaceutical agents, soluble biologics or as surface coatings for medical devices.

  19. Gene encoding herbicide safener binding protein

    DOE Patents [OSTI]

    Walton, Jonathan D.; Scott-Craig, John S.

    1999-01-01

    The cDNA encoding safener binding protein (SafBP), also referred to as SBP1, is set forth in FIG. 5 and SEQ ID No. 1. The deduced amino acid sequence is provided in FIG. 5 and SEQ ID No. 2. Methods of making and using SBP1 and SafBP to alter a plant's sensitivity to certain herbicides or a plant's responsiveness to certain safeners are also provided, as well as expression vectors, transgenic plants or other organisms transfected with said vectors and seeds from said plants.

  20. Polynucleotides encoding TRF1 binding proteins

    DOE Patents [OSTI]

    Campisi, Judith (Berkeley, CA); Kim, Sahn-Ho (Albany, CA)

    2002-01-01

    The present invention provides a novel telomere associated protein (Trf1-interacting nuclear protein 2 "Tin2") that hinders the binding of Trf1 to its specific telomere repeat sequence and mediates the formation of a Tin2-Trf1-telomeric DNA complex that limits telomerase access to the telomere. Also included are the corresponding nucleic acids that encode the Tin2 of the present invention, as well as mutants of Tin2. Methods of making, purifying and using Tin2 of the present invention are described. In addition, drug screening assays to identify drugs that mimic and/or complement the effect of Tin2 are presented.

  1. NSLS-II Transport Line Progress

    SciTech Connect (OSTI)

    Fliller R. P.; Wahl, W.; Anderson, A.; Benish, B.; DeBoer, W.; Ganetis, G.; Heese, R.; Hseuh, H.-C.; Hu, J.-P.; Johanson, M.P.; Kosciuk, B.N.; Padrazo, D.; Roy, K.; Shaftan, T.; Singh, O.; Tuozzolo, J.; Wang, G.

    2012-05-20

    The National Synchrotron Light Source II (NSLS-II) is a state-of-the-art 3-GeV third generation light source currently under construction at Brookhaven National Laboratory. The NSLS-II injection system consists of a 200 MeV linac, a 3-GeV booster synchrotron and associated transfer lines. The first part of the Linac to Booster Transport (LBT) line has been installed for linac commissioning. This part includes all components necessary to commission the NSLS-II linac. The second part of this transport line is undergoing installation. Initial results of hardware commissioning will be discussed. The Booster to Storage Ring (BSR) transport line underwent a design review. The first part of the BSR transport line, consisting of all components necessary to commission the booster will be installed in 2012 for booster commissioning. We report on the final design of the BSR line along with the plan to commission the booster.

  2. Single transmission line data acquisition system

    DOE Patents [OSTI]

    Fasching, George E. (Morgantown, WV)

    1984-01-01

    A single transmission line interrogated multiple channel data acquisition system is provided in which a plurality of remote station/sensors monitor specific process variables and transmit measurement values over the single transmission line to a master station when addressed by the master station. Power for all remote stations (up to 980) is provided by driving the line with constant voltage supplied from the master station and automatically maintained independent of the number of remote stations directly connected to the line. The transmission line can be an RG-62 coaxial cable with lengths up to about 10,000 feet with branches up to 500 feet. The remote stations can be attached randomly along the line. The remote stations can be scanned at rates up to 980 channels/second.

  3. EM Recovery Act Top Line Messages | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Top Line Messages EM Recovery Act Top Line Messages The lastest Recovery Act performance related information and metrics. PDF icon EM Recovery Act Top Line Messages - April, 2013...

  4. Transmission Line Security Monitor: Final Report

    SciTech Connect (OSTI)

    John Svoboda

    2011-04-01

    The Electric Power Transmission Line Security Monitor System Operational Test is a project funded by the Technical Support Working Group (TSWG). TSWG operates under the Combating Terrorism Technical Support Office that functions under the Department of Defense. The Transmission Line Security Monitor System is based on technology developed by Idaho National Laboratory. The technology provides a means for real-time monitoring of physical threats and/or damage to electrical transmission line towers and conductors as well as providing operational parameters to transmission line operators to optimize transmission line operation. The end use is for monitoring long stretches of transmission lines that deliver electrical power from remote generating stations to cities and industry. These transmission lines are generally located in remote transmission line corridors where security infrastructure may not exist. Security and operational sensors in the sensor platform on the conductors take power from the transmission line and relay security and operational information to operations personnel hundreds of miles away without relying on existing infrastructure. Initiated on May 25, 2007, this project resulted in pre-production units tested in realistic operational environments during 2010. A technology licensee, Lindsey Manufacturing of Azusa California, is assisting in design, testing, and ultimately production. The platform was originally designed for a security monitoring mission, but it has been enhanced to include important operational features desired by electrical utilities.

  5. Detonator comprising a nonlinear transmission line

    DOE Patents [OSTI]

    Elizondo-Decanini, Juan M

    2014-12-30

    Detonators are described herein. In a general embodiment, the detonator includes a nonlinear transmission line that has a variable capacitance. Capacitance of the nonlinear transmission line is a function of voltage on the nonlinear transmission line. The nonlinear transmission line receives a voltage pulse from a voltage source and compresses the voltage pulse to generate a trigger signal. Compressing the voltage pulse includes increasing amplitude of the voltage pulse and decreasing length of the voltage pulse in time. An igniter receives the trigger signal and detonates an explosive responsive to receipt of the trigger signal.

  6. Strengthening Line Management Oversight and Federal Monitoring...

    Office of Environmental Management (EM)

    5 iii Acronyms DOE U.S. Department of Energy DSA Documented Safety Analysis HA Hazard Analysis LOI Line of Inquiry SAC Specific Administrative Control SRP Standard...

  7. Strengthening Line Management Oversight and Federal Monitoring...

    Office of Environmental Management (EM)

    for DOE nuclear facilities. This SRP, Safety Basis Program Review, contains five volumes to help strengthen the technical rigor of line management oversight and federal...

  8. Northern Pass Transmission Line Project Environmental Impact...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Northern Pass Transmission Line Project Environmental Impact Statement: Announcement of Change in Public Meeting Location: Federal Register Notice Volume 78, No. 181 - September ...

  9. DOE Sustainability Reporting Open Line Help Call

    Broader source: Energy.gov [DOE]

    The Sustainability Performance Office (SPO) sponsors open line help calls to assist DOE sites and national laboratories with the annual sustainability reporting process. Representatives from the...

  10. Supervisory Transmission Lines and Substation Maintenance Manager

    Broader source: Energy.gov [DOE]

    (See Frequently Asked Questions for more information). Where would I be working? Western Area Power Administration Desert Southwest Region Transmission Lines and Substation Maintenance (G5200) 615...

  11. Secretary Bodman Praises Western States' "Frontier Line"

    Broader source: Energy.gov [DOE]

    WASHINGTON, DC - Secretary of Energy Samuel W. Bodman today released the following statement regarding the “Frontier Line” announced by Governors Arnold Schwarzenegger (CA), Kenny Guinn (NV), Jon...

  12. Strengthening Line Management Oversight and Federal Monitoring...

    Office of Environmental Management (EM)

    Management JCO Justification for Continued Operation LOI Line of Inquiry PAAA Price Anderson Amendments Act PDSA Preliminary Documented Safety Analysis PHA Preliminary Hazards...

  13. X-ray line polarization spectroscopy of Li-like satellite line spectra

    Office of Scientific and Technical Information (OSTI)

    (Journal Article) | SciTech Connect Journal Article: X-ray line polarization spectroscopy of Li-like satellite line spectra Citation Details In-Document Search Title: X-ray line polarization spectroscopy of Li-like satellite line spectra We apply the magnetic-sublevel atomic kinetics model POLAR to the calculation of polarization properties of satellite lines in Li-like Si driven by subpicosecond-duration laser pulses. We identify spectral lines whose polarization can serve as a marker of

  14. Method and apparatus for detecting chemical binding

    DOE Patents [OSTI]

    Warner, Benjamin P.; Havrilla, George J.; Miller, Thomasin C.; Wells, Cyndi A.

    2007-07-10

    The method for screening binding between a target binder and potential pharmaceutical chemicals involves sending a solution (preferably an aqueous solution) of the target binder through a conduit to a size exclusion filter, the target binder being too large to pass through the size exclusion filter, and then sending a solution of one or more potential pharmaceutical chemicals (preferably an aqueous solution) through the same conduit to the size exclusion filter after target binder has collected on the filter. The potential pharmaceutical chemicals are small enough to pass through the filter. Afterwards, x-rays are sent from an x-ray source to the size exclusion filter, and if the potential pharmaceutical chemicals form a complex with the target binder, the complex produces an x-ray fluorescence signal having an intensity that indicates that a complex has formed.

  15. Method And Apparatus For Detecting Chemical Binding

    DOE Patents [OSTI]

    Warner, Benjamin P.; Havrilla, George J.; Miller, Thomasin C.; Wells, Cyndi A.

    2005-02-22

    The method for screening binding between a target binder and potential pharmaceutical chemicals involves sending a solution (preferably an aqueous solution) of the target binder through a conduit to a size exclusion filter, the target binder being too large to pass through the size exclusion filter, and then sending a solution of one or more potential pharmaceutical chemicals (preferably an aqueous solution) through the same conduit to the size exclusion filter after target binder has collected on the filter. The potential pharmaceutical chemicals are small enough to pass through the filter. Afterwards, x-rays are sent from an x-ray source to the size exclusion filter, and if the potential pharmaceutical chemicals form a complex with the target binder, the complex produces an x-ray fluorescence signal having an intensity that indicates that a complex has formed.

  16. Neptunium Binding Kinetics with Arsenazo(III)

    SciTech Connect (OSTI)

    Leigh R. Martin; Aaron T. Johnson; Stephen P. Mezyk

    2014-08-01

    This document has been prepared to meet FCR&D level 2 milestone M2FT-14IN0304021, “Report on the results of actinide binding kinetics with aqueous phase complexants” This work was carried out under the auspices of the Thermodynamics and Kinetics of Advanced Separations Systems FCR&D work package. The report details kinetics experiments that were performed to measure rates of aqueous phase complexation for pentavalent neptunium with the chromotropic dye Arsenazo III (AAIII). The studies performed were designed to determine how pH, ionic strength and AAIII concentration may affect the rate of the reaction. A brief comparison with hexavalent neptunium is also made. It was identified that as pH was increased the rate of reaction also increased, however increasing the ionic strength and concentration of AAIII had the opposite effect. Interestingly, the rate of reaction of Np(VI) with AAIII was found to be slower than that of the Np(V) reaction.

  17. Hardware device to physical structure binding and authentication

    DOE Patents [OSTI]

    Hamlet, Jason R.; Stein, David J.; Bauer, Todd M.

    2013-08-20

    Detection and deterrence of device tampering and subversion may be achieved by including a cryptographic fingerprint unit within a hardware device for authenticating a binding of the hardware device and a physical structure. The cryptographic fingerprint unit includes an internal physically unclonable function ("PUF") circuit disposed in or on the hardware device, which generate an internal PUF value. Binding logic is coupled to receive the internal PUF value, as well as an external PUF value associated with the physical structure, and generates a binding PUF value, which represents the binding of the hardware device and the physical structure. The cryptographic fingerprint unit also includes a cryptographic unit that uses the binding PUF value to allow a challenger to authenticate the binding.

  18. Soliton production with nonlinear homogeneous lines

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Elizondo-Decanini, Juan M.; Coleman, Phillip D.; Moorman, Matthew W.; Petney, Sharon Joy Victor; Dudley, Evan C.; Youngman, Kevin; Penner, Tim Dwight; Fang, Lu; Myers, Katherine M.

    2015-11-24

    Low- and high-voltage Soliton waves were produced and used to demonstrate collision and compression using diode-based nonlinear transmission lines. Experiments demonstrate soliton addition and compression using homogeneous nonlinear lines. We built the nonlinear lines using commercially available diodes. These diodes are chosen after their capacitance versus voltage dependence is used in a model and the line design characteristics are calculated and simulated. Nonlinear ceramic capacitors are then used to demonstrate high-voltage pulse amplification and compression. The line is designed such that a simple capacitor discharge, input signal, develops soliton trains in as few as 12 stages. We also demonstrated outputmore » voltages in excess of 40 kV using Y5V-based commercial capacitors. The results show some key features that determine efficient production of trains of solitons in the kilovolt range.« less

  19. Soliton production with nonlinear homogeneous lines

    SciTech Connect (OSTI)

    Elizondo-Decanini, Juan M.; Coleman, Phillip D.; Moorman, Matthew W.; Petney, Sharon Joy Victor; Dudley, Evan C.; Youngman, Kevin; Penner, Tim Dwight; Fang, Lu; Myers, Katherine M.

    2015-11-24

    Low- and high-voltage Soliton waves were produced and used to demonstrate collision and compression using diode-based nonlinear transmission lines. Experiments demonstrate soliton addition and compression using homogeneous nonlinear lines. We built the nonlinear lines using commercially available diodes. These diodes are chosen after their capacitance versus voltage dependence is used in a model and the line design characteristics are calculated and simulated. Nonlinear ceramic capacitors are then used to demonstrate high-voltage pulse amplification and compression. The line is designed such that a simple capacitor discharge, input signal, develops soliton trains in as few as 12 stages. We also demonstrated output voltages in excess of 40 kV using Y5V-based commercial capacitors. The results show some key features that determine efficient production of trains of solitons in the kilovolt range.

  20. 2014 EIA-821 SURVEY: LINE-BY-LINE REFERENCE GUIDE Kerosene

    Gasoline and Diesel Fuel Update (EIA)

    2014 EIA-821 SURVEY: LINE-BY-LINE REFERENCE GUIDE Kerosene Line No. Sold directly to consumers for: 1 Residential Use (Non-Farm): * Backup generator * Home heating and cooking * Personal lawn equipment * Exclude: Apartment buildings and Farmhouses 2 Commercial Use: * Apartment building * Bank * Casino * Church * College/School/Institution * Department/Retail store * Environmental clean-up service * Flushing fuel lines * Forestry service * Golf course * Government (federal, state, local, and

  1. The Effects of Somatic Hypermutation on Neutralization and Binding...

    Office of Scientific and Technical Information (OSTI)

    Hypermutation on Neutralization and Binding in the PGT121 Family of Broadly Neutralizing HIV Antibodies Citation Details In-Document Search Title: The Effects of Somatic...

  2. U-183: ISC BIND DNS Resource Records Handling Vulnerability

    Broader source: Energy.gov [DOE]

    This problem was uncovered while testing with experimental DNS record types. It is possible to add records to BIND with null (zero length) rdata fields.

  3. Developing Adnectins That Target SRC Co-Activator Binding to...

    Office of Scientific and Technical Information (OSTI)

    Developing Adnectins That Target SRC Co-Activator Binding to PXR: A Structural Approach toward Understanding Promiscuity of PXR Citation Details In-Document Search Title: ...

  4. Structural and functional analysis of FIP2 binding to theendosome...

    Office of Scientific and Technical Information (OSTI)

    Journal Article: Structural and functional analysis of FIP2 binding to the endosome-localised Rab25 GTPase Citation Details In-Document Search Title: Structural and functional ...

  5. Design of Bcl-2 and Bcl-xL Inhibitors with Subnanomolar Binding Affinities Based upon a New Scaffold

    SciTech Connect (OSTI)

    Zhou, Haibin; Chen, Jianfang; Meagher, Jennifer L.; Yang, Chao-Yie; Aguilar, Angelo; Liu, Liu; Bai, Longchuan; Cong, Xin; Cai, Qian; Fang, Xueliang; Stuckey, Jeanne A.; Wang, Shaomeng

    2014-10-02

    Employing a structure-based strategy, we have designed a new class of potent small-molecule inhibitors of the anti-apoptotic proteins Bcl-2 and Bcl-xL. An initial lead compound with a new scaffold was designed based upon the crystal structure of Bcl-xL and U.S. Food and Drug Administration (FDA) approved drugs and was found to have an affinity of 100 {micro}M for both Bcl-2 and Bcl-xL. Linking this weak lead to another weak-affinity fragment derived from Abbott's ABT-737 led to an improvement of the binding affinity by a factor of >10,000. Further optimization ultimately yielded compounds with subnanomolar binding affinities for both Bcl-2 and Bcl-xL and potent cellular activity. The best compound (21) binds to Bcl-xL and Bcl-2 with K{sub i} < 1 nM, inhibits cell growth in the H146 and H1417 small-cell lung cancer cell lines with IC{sub 50} values of 60-90 nM, and induces robust cell death in the H146 cancer cell line at 30-100 nM.

  6. Getting to the starting line | Jefferson Lab

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Getting to the starting line November 12, 2009 There are some analogies that keep on turning up when we describe what is going on either in the world or in our lives. The analogy of a race is one of those. When we achieve a great victory or complete a project, we may speak of crossing the finishing line. However, it is also recognized that it's often an achievement just to get to the starting line. About a year ago, we discussed with the Science Council what was happening with the free- electron

  7. Computing Criticality of Lines in Power Systems

    SciTech Connect (OSTI)

    Pinar, Ali; Reichert, Adam; Lesieutre, Bernard

    2006-10-13

    We propose a computationally efficient method based onnonlinear optimization to identify critical lines, failure of which cancause severe blackouts. Our method computes criticality measure for alllines at a time, as opposed to detecting a single vulnerability,providing a global view of the system. This information on criticality oflines can be used to identify multiple contingencies by selectivelyexploring multiple combinations of broken lines. The effectiveness of ourmethod is demonstrated on the IEEE 30 and 118 bus systems, where we canvery quickly detect the most critical lines in the system and identifysevere multiple contingencies.

  8. Multidomain Carbohydrate-binding Proteins Involved in Bacteroides

    Office of Scientific and Technical Information (OSTI)

    thetaiotaomicron Starch Metabolism (Journal Article) | SciTech Connect Multidomain Carbohydrate-binding Proteins Involved in Bacteroides thetaiotaomicron Starch Metabolism Citation Details In-Document Search Title: Multidomain Carbohydrate-binding Proteins Involved in Bacteroides thetaiotaomicron Starch Metabolism Authors: Cameron, Elizabeth A. ; Maynard, Mallory A. ; Smith, Christopher J. ; Smith, Thomas J. ; Koropatkin, Nicole M. ; Martens, Eric C. [1] ; Danforth) [2] + Show Author

  9. Electrical Transmission Line Diametrical Retention Mechanism

    DOE Patents [OSTI]

    Hall, David R.; Hall, Jr., H. Tracy; Pixton, David; Dahlgren, Scott; Sneddon, Cameron; Briscoe, Michael; Fox, Joe

    2006-01-03

    The invention is a mechanism for retaining an electrical transmission line. In one embodiment of the invention it is a system for retaining an electrical transmission line within downhole components. The invention allows a transmission line to be attached to the internal diameter of drilling components that have a substantially uniform drilling diameter. In accordance with one aspect of the invention, the system includes a plurality of downhole components, such as sections of pipe in a drill string, drill collars, heavy weight drill pipe, and jars. The system also includes a coaxial cable running between the first and second end of a drill pipe, the coaxial cable having a conductive tube and a conductive core within it. The invention allows the electrical transmission line to withstand the tension and compression of drill pipe during routine drilling cycles.

  10. Temperatures in the blast furnace refractory lining

    SciTech Connect (OSTI)

    Hebel, R.; Streuber, C.; Steiger, R.; Jeschar, R.

    1995-12-01

    The campaign life duration of a blast furnace is mainly determined by the condition of the refractory lining in heavy-duty zones such as the hearth, bosh, belly and lower stack. To achieve a desired lifetime, the temperature of the lining in these areas thereby proved to be the decisive controllable parameter. Low operating temperatures result in prolonged service life and are attained through high cooling efficiency. Besides the refractory grade chosen, the wear profile is mainly determined by the type of cooling system applied and the cooling intensity. Therefore, an appropriate compromise between long service life and energy losses has to be found in each case. In order to predict the service life of a lining it is important to know the wear condition at all times during the campaign. The paper describes the approaches the authors have made so far on European blast furnaces, on a theoretical and practical basis, on how to analyze the lining wear.

  11. Line Environment, Safety and Health Oversight

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    1997-06-26

    Sets forth the Department's expectations line management environment, safety and health (ES&H) oversight and for the use of contractor self-assessment programs as the cornerstone for this oversight. Canceled by DOE O 226.1.

  12. DNA Assembly Line for Nano-Construction

    ScienceCinema (OSTI)

    Oleg Gang

    2010-01-08

    Building on the idea of using DNA to link up nanoparticles scientists at Brookhaven National Lab have designed a molecular assembly line for high-precision nano-construction. Nanofabrication is essential for exploiting the unique properties of nanoparticl

  13. Ames Lab 101: Next Generation Power Lines

    ScienceCinema (OSTI)

    Russell, Alan

    2012-08-29

    Ames Laboratory scientist Alan Russell discusses the need to develop new power lines that are stronger and more conductive as a way to address the problem of the nation's aging and inadequate power grid.

  14. factsheet - trees and power lines - July 2008

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Trees and power lines July 2008 This summer, BPA maintenance crews are inspecting and clearing high-growing vegetation from all 8,500- miles (15,000 circuit miles) of our...

  15. On-line chemical composition analyzer development

    SciTech Connect (OSTI)

    Garrison, A.A.

    1993-01-01

    This report relates to the development of an on-line Raman analyzer for control of a distillation column. It is divided into: program issues, experimental control system evaluation, energy savings analysis, and reliability analysis. (DLC)

  16. Electrician - Foreman II (Transmission Lines & Substations) ...

    Broader source: Energy.gov (indexed) [DOE]

    Region Transmission Lines and Substation Maintenance (G5200) 615 S. 43rd Avenue Phoenix, AZ 85009 Duty Location is Page, AZ Find out more about living conditions at this...

  17. Design of the ILC RTML Extraction Lines

    SciTech Connect (OSTI)

    Seletskiy, S.; Tenenbaum, P.; Walz, D.; Solyak, N.; /Fermilab

    2011-10-17

    The ILC [1] Damping Ring to the Main Linac beamline (RTML) contains three extraction lines (EL). Each EL can be used both for an emergency abort dumping of the beam and tune-up continual train-by-train extraction. Two of the extraction lines are located downstream of the first and second stages of the RTML bunch compressor, and must accept both compressed and uncompressed beam with energy spreads of 2.5% and 0.15%, respectively. In this paper we report on an optics design that allowed minimizing the length of the extraction lines while offsetting the beam dumps from the main line by the distance required for acceptable radiation levels in the service tunnel. The proposed extraction lines can accommodate beams with different energy spreads while at the same time providing the beam size acceptable for the aluminum dump window. The RTML incorporates three extraction lines, which can be used for either an emergency beam abort or for a train-by-train extraction. The first EL is located downstream of the Damping Ring extraction arc. The other two extraction lines are located downstream of each stage of the two-stage bunch compressor. The first extraction line (EL1) receives 5GeV beam with an 0.15% energy spread. The extraction line located downstream of the first stage of bunch compressor (ELBC1) receives both compressed and uncompressed beam, and therefore must accept beam with both 5 and 4.88GeV energy, and 0.15% and 2.5% energy spread, respectively. The extraction line located after the second stage of the bunch compressor (ELBC2) receives 15GeV beam with either 0.15 or 1.8% energy spread. Each of the three extraction lines is equipped with the 220kW aluminum ball dump, which corresponds to the power of the continuously dumped beam with 5GeV energy, i.e., the beam trains must be delivered to the ELBC2 dump at reduced repetition rate.

  18. Load-resistant coaxial transmission line

    DOE Patents [OSTI]

    Hall, David R.; Fox, Joe

    2006-01-03

    A transmission line for downhole tools that make up all or part of a tool string for drilling and production of oil, gas, and geothermal wells that can withstand the dynamic gravitational forces and other accelerations associated with downhole excavations. The transmission line has a metal tube, or outer conductor, that houses a coaxial wire inner conductor. A non-metallic dielectric material is interposed between the inner and outer conductors. The outer and inner conductors and the dielectric are sufficiently compressed together so that independent motion between them is abated. Compression of the components of the transmission line may be achieved by drawing the transmission through one or more dies in order to draw down the outer conductor onto the dielectric, or by expanding the inner conductor against the dielectric using a mandrel or hydraulic pressure. Non-metallic bead segments may be used in aid of the compression necessary to resist the dynamic forces and accelerations of drilling.

  19. Dynamic Line Rating: Research and Policy Evaluation

    SciTech Connect (OSTI)

    Jake P. Gentle; Kurt S. Myers; Michael R. West

    2014-07-01

    Dynamic Line Rating (DLR) is a smart grid technology that allows the rating of electrical conductors to be increased based on local weather conditions. Overhead lines are conventionally given a conservative rating based on worst case scenarios. We demonstrate that observing the conditions in real time leads to additional capacity and safer operation. This paper provides a report of a pioneering scheme in the United States of America in which DLR has been applied. Thereby, we demonstrate that observing the local weather conditions in real time leads to additional capacity and safer operation. Secondly, we discuss limitations involved. In doing so, we arrive at novel insights which will inform and improve future DLR projects. Third, we provide a policy background and discussion to clarify the technology’s potential and identifies barriers to the imminent adoption of dynamic line rating systems. We provide suggestions for regulatory bodies about possible improvements in policy to encourage adoption of this beneficial technology.

  20. Nuclear binding energy and symmetry energy of nuclear matter with modern nucleon-nucleon potentials

    SciTech Connect (OSTI)

    Hassaneen, Kh.S.A.; Abo-Elsebaa, H.M.; Sultan, E.A.; Mansour, H.M.M.

    2011-03-15

    Research Highlights: > The nuclear matter is studied within the Brueckner-Hartree-Fock (BHF) approach employing the most recent accurate nucleon-nucleon potentials. > The results come out by approximating the single particle self-consistent potential with a parabolic form. > We discuss the current status of the Coester line, i.e., density and energy of the various saturation points being strongly linearly correlated. > The nuclear symmetry energy is calculated as the difference between the binding energy of pure neutron matter and that of symmetric nuclear matter. - Abstract: The binding energy of nuclear matter at zero temperature in the Brueckner-Hartree-Fock approximation with modern nucleon-nucleon potentials is studied. Both the standard and continuous choices of single particle energies are used. These modern nucleon-nucleon potentials fit the deuteron properties and are phase shifts equivalent. Comparison with other calculations is made. In addition we present results for the symmetry energy obtained with different potentials, which is of great importance in astrophysical calculation.

  1. Botas line repaired ahead of schedule

    SciTech Connect (OSTI)

    Not Available

    1991-11-01

    This paper reports on damaged sections of Botas' dual 30-in. gas line carrying USSR gas across the Marmara Sea to Turkey that were replaced 30 days ahead of contract schedule. Tefken Construction and Installation Co., Inc., Istanbul, working under a $4-million contract, replaced two sections on one of the dual lines near Ambarli, Turkey, within a two-month period. The offshore system stretches 33-mi under the Marmara Sea with some pipe laid at 262-ft maximum water depths. The scope of the project was to replace a 426-ft offshore approach to the northern shoreline and a 984-ft onshore section, which were damaged by a submarine landslide.

  2. EC Transmission Line Risk Identification and Analysis

    SciTech Connect (OSTI)

    Bigelow, Tim S

    2012-04-01

    The purpose of this document is to assist in evaluating and planning for the cost, schedule, and technical project risks associated with the delivery and operation of the EC (Electron cyclotron) transmission line system. In general, the major risks that are anticipated to be encountered during the project delivery phase associated with the implementation of the Procurement Arrangement for the EC transmission line system are associated with: (1) Undefined or changing requirements (e.g., functional or regulatory requirements) (2) Underperformance of prototype, first unit, or production components during testing (3) Unavailability of qualified vendors for critical components Technical risks associated with the design and operation of the system are also identified.

  3. Concurrent Wind Cooling in Power Transmission Lines

    SciTech Connect (OSTI)

    Jake P Gentle

    2012-08-01

    Idaho National Laboratory and the Idaho Power Company, with collaboration from Idaho State University, have been working on a project to monitor wind and other environmental data parameters along certain electrical transmission corridors. The combination of both real-time historical weather and environmental data is being used to model, validate, and recommend possibilities for dynamic operations of the transmission lines for power and energy carrying capacity. The planned results can also be used to influence decisions about proposed design criteria for or upgrades to certain sections of the transmission lines.

  4. 1 and 2-Dimensional Line Transfer Package

    Energy Science and Technology Software Center (OSTI)

    1990-07-01

    LXF1D is a one dimensional steady-state line transfer package designed to handle: overlapping and or interacting lines, planar, cylindrical, spherical (and special) geometries, doppler shifts, complete redistribution (CRD), partial redistribution (PRD). PRD requires the use of REDIST or some other package to produce emission profiles. LXF2D is a two dimensional version of LXF1D for xy and rz geometries. Both LXF1D and LXF2D are designed to be added to existing non-local thermodynamic equilibrium (NLTE) codes withmore » a minimum of effort.« less

  5. Increasing the Capacity of Existing Power Lines

    Energy Savers [EERE]

    ENERGY AND ENVIRONMENT Continued next page In the continental United States, some 500 power companies operate a complex network of more than 160,000 miles of high-voltage trans- mission lines known as "the grid." The capacity of the grid has been largely unchanged for decades and needs to expand to accommodate new power plants and renewable energy projects. The difference in time and cost between using existing transmission lines or the construction of new ones can make or break plans

  6. Metal binding proteins, recombinant host cells and methods

    DOE Patents [OSTI]

    Summers, Anne O.; Caguiat, Jonathan J.

    2004-06-15

    The present disclosure provides artificial heavy metal binding proteins termed chelons by the inventors. These chelons bind cadmium and/or mercuric ions with relatively high affinity. Also disclosed are coding sequences, recombinant DNA molecules and recombinant host cells comprising those recombinant DNA molecules for expression of the chelon proteins. In the recombinant host cells or transgenic plants, the chelons can be used to bind heavy metals taken up from contaminated soil, groundwater or irrigation water and to concentrate and sequester those ions. Recombinant enteric bacteria can be used within the gastrointestinal tracts of animals or humans exposed to toxic metal ions such as mercury and/or cadmium, where the chelon recombinantly expressed in chosen in accordance with the ion to be rededicated. Alternatively, the chelons can be immobilized to solid supports to bind and concentrate heavy metals from a contaminated aqueous medium including biological fluids.

  7. Linear Scaling of the Exciton Binding Energy versus the Band...

    Office of Scientific and Technical Information (OSTI)

    Linear Scaling of the Exciton Binding Energy versus the Band Gap of Two-Dimensional Materials This content will become publicly available on August 6, 2016 Prev Next Title:...

  8. Plains & Eastern Clean Line Transmission Line- Part 2 Application: Comments Received on the Updated Application

    Broader source: Energy.gov [DOE]

    In December 2014, DOE requested additional information from Clean Line Energy Partners, LLC to supplement and update its original Plains and Eastern Clean Line application. The updated Part 2 application and other documentation were available for a public comment period that closed on July 13, 2015. Comments were posted as they were received.

  9. Self-Aligning Mirror Mechanism for Transmission Line Offset Correction...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Self-Aligning Mirror Mechanism for Transmission Line Offset Correction The Self-Aligning Mirror Mechanism for Transmission Line Offset Correction is a self-aligning mechanism which...

  10. Independent Oversight Evaluation of Line Self-Assessments of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Evaluation of Line Self-Assessments of Safety Conscious Work Environment - February 2014 Independent Oversight Evaluation of Line Self-Assessments of Safety Conscious Work...

  11. EIS-0514: Colusa-Sutter 500-kilovolt Transmission Line Project...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    514: Colusa-Sutter 500-kilovolt Transmission Line Project; Colusa and Sutter Counties, California EIS-0514: Colusa-Sutter 500-kilovolt Transmission Line Project; Colusa and Sutter ...

  12. Line spectrum and ion temperature measurements from tungsten...

    Office of Scientific and Technical Information (OSTI)

    Line spectrum and ion temperature measurements from tungsten ions at low ionization stages ... Citation Details In-Document Search Title: Line spectrum and ion temperature measurements ...

  13. PVM Lines and Services LLC aka PVML Photovoltaics | Open Energy...

    Open Energy Info (EERE)

    PVM Lines and Services LLC aka PVML Photovoltaics Jump to: navigation, search Name: PVM Lines and Services LLC (aka PVML Photovoltaics) Place: Princeton, New Jersey Zip: 8540...

  14. Arizona Transmission Line Siting Committee | Open Energy Information

    Open Energy Info (EERE)

    Line Siting Committee Jump to: navigation, search Name: Arizona Transmission Line Siting Committee Abbreviation: TLSC Address: 1200 West Washington Street Place: Phoenix, Arizona...

  15. Sandia Energy - Experts Call for Triple Bottom Line Approach...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Experts Call for Triple Bottom Line Approach to National Energy Policy Home Energy News News & Events Mission VisionMission Experts Call for Triple Bottom Line Approach to...

  16. Development of aerogel-lined targets for inertial confinement...

    Office of Scientific and Technical Information (OSTI)

    ThesisDissertation: Development of aerogel-lined targets for inertial confinement fusion experiments Citation Details In-Document Search Title: Development of aerogel-lined ...

  17. Sequestering Uranium from Seawater: Binding Strength and Modes of Uranyl

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Complexes with Glutarimidedioxime Sequestering Uranium from Seawater: Binding Strength and Modes of Uranyl Complexes with Glutarimidedioxime Sequestering Uranium from Seawater: Binding Strength and Modes of Uranyl Complexes with Glutarimidedioxime Print Sunday, 14 October 2012 00:00 The ocean is an important source of uranium if it can be extracted economically. Extraction of uranium from seawater is very challenging, not only because it is in an extremely low concentration, but also because

  18. Briefly Bound to Activate: Transient Binding of a Second Catalytic

    Office of Scientific and Technical Information (OSTI)

    Magnesium Activates the Structure and Dynamics of CDK2 Kinase for Catalysis (Journal Article) | SciTech Connect SciTech Connect Search Results Journal Article: Briefly Bound to Activate: Transient Binding of a Second Catalytic Magnesium Activates the Structure and Dynamics of CDK2 Kinase for Catalysis Citation Details In-Document Search Title: Briefly Bound to Activate: Transient Binding of a Second Catalytic Magnesium Activates the Structure and Dynamics of CDK2 Kinase for Catalysis We have

  19. Fragment-Based Exploration of Binding Site Flexibility in Mycobacterium

    Office of Scientific and Technical Information (OSTI)

    tuberculosis BioA (Journal Article) | SciTech Connect Fragment-Based Exploration of Binding Site Flexibility in Mycobacterium tuberculosis BioA Citation Details In-Document Search Title: Fragment-Based Exploration of Binding Site Flexibility in Mycobacterium tuberculosis BioA Authors: Dai, Ran ; Geders, Todd W. ; Liu, Feng ; Park, Sae Woong ; Schnappinger, Dirk ; Aldrich, Courtney C. ; Finzel, Barry C. [1] ; Weill-Med) [2] + Show Author Affiliations (UMM) ( Publication Date: 2015-09-29 OSTI

  20. DNA-Binding Mechanism in Prokaryotic Partition Complex Formation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    DNA-Binding Mechanism in Prokaryotic Partition Complex Formation DNA-Binding Mechanism in Prokaryotic Partition Complex Formation Print Wednesday, 29 March 2006 00:00 The faithful inheritance of genetic information, essential for all organisms, requires accurate movement and positioning of replicated DNA to daughter cells during cell division. In cells without distinct nuclei (prokaryotes), this process, called partition or segregation, is mediated by par systems. The prototype system of

  1. Characterization of Selective Binding of Alkali Cations with Carboxylate

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Characterization of Selective Binding of Alkali Cations with Carboxylate Characterization of Selective Binding of Alkali Cations with Carboxylate Print Wednesday, 24 September 2008 00:00 During its lifetime, a cell spends a considerable fraction of its energy pumping sodium and calcium out and potassium in. This balancing process is similar to that found in the coils of the DNA double helix, where specific ions nestle and help stabilize this macromolecule. These are only two examples of

  2. Binding Behavior of Dopamine Transporter Key to Understanding Chemical

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Reactions in the Brain Binding Behavior of Dopamine Transporter Key to Understanding Chemical Reactions in the Brain Binding Behavior of Dopamine Transporter Key to Understanding Chemical Reactions in the Brain Print Wednesday, 09 December 2015 00:00 Most people have heard of adrenaline, the chemical that causes the "fight or flight" reaction in humans. Most people have also heard of the chemical substances cocaine and methamphetamine, which also elicit a particular (perhaps

  3. Computational Biology: A Recipe for Ligand-Binding Proteins

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Computational Biology: A Recipe for Ligand-Binding Proteins Authors: Ghirlanda, G. Title: Computational Biology: A Recipe for Ligand-Binding Proteins Source: Nature Year: 2013 Volume: 501 Pages: 177-178 ABSTRACT: Cellular cross-talk, enzymatic catalysis and regulation of gene expression all depend on molecular recognition. A method that allows the design of proteins with desired recognition sites could thus be revolutionary Date of online publication: Thu, 2013-09-12 Link online:

  4. Structural and functional analysis of FIP2 binding to the

    Office of Scientific and Technical Information (OSTI)

    endosome-localised Rab25 GTPase (Journal Article) | SciTech Connect Journal Article: Structural and functional analysis of FIP2 binding to the endosome-localised Rab25 GTPase Citation Details In-Document Search Title: Structural and functional analysis of FIP2 binding to the endosome-localised Rab25 GTPase Authors: Lall, Patrick ; Horgan, Conor P. ; Oda, Shunichiro ; Franklin, Edward ; Sultana, Azmiri ; Hanscom, Sara R. ; McCaffrey, Mary W. ; Khan, Amir R. [1] ; Cork) [2] + Show Author

  5. Structural basis for biomolecular recognition in overlapping binding sites

    Office of Scientific and Technical Information (OSTI)

    in a diiron enzyme system (Journal Article) | SciTech Connect Structural basis for biomolecular recognition in overlapping binding sites in a diiron enzyme system Citation Details In-Document Search Title: Structural basis for biomolecular recognition in overlapping binding sites in a diiron enzyme system Authors: Acheson, Justin F. ; Bailey, Lucas J. ; Elsen, Nathaniel L. ; Fox, Brian G. [1] + Show Author Affiliations UW Publication Date: 2016-01-22 OSTI Identifier: 1229904 Resource Type:

  6. MCM ring hexamerization is a prerequisite for DNA-binding

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Froelich, Clifford A.; Nourse, Amanda; Enemark, Eric J.

    2015-09-13

    The hexameric Minichromosome Maintenance (MCM) protein complex forms a ring that unwinds DNA at the replication fork in eukaryotes and archaea. Our recent crystal structure of an archaeal MCM N-terminal domain bound to single-stranded DNA (ssDNA) revealed ssDNA associating across tight subunit interfaces but not at the loose interfaces, indicating that DNA-binding is governed not only by the DNA-binding residues of the subunits (MCM ssDNA-binding motif, MSSB) but also by the relative orientation of the subunits. We now extend these findings to show that DNA-binding by the MCM N-terminal domain of the archaeal organism Pyrococcus furiosus occurs specifically in themore » hexameric oligomeric form. We show that mutants defective for hexamerization are defective in binding ssDNA despite retaining all the residues observed to interact with ssDNA in the crystal structure. One mutation that exhibits severely defective hexamerization and ssDNA-binding is at a conserved phenylalanine that aligns with the mouse Mcm4(Chaos3) mutation associated with chromosomal instability, cancer, and decreased intersubunit association.« less

  7. DOE Sustainability Reporting Open Line Help Call

    Broader source: Energy.gov [DOE]

    The Sustainability Performance Office (SPO) sponsors open line help calls to assist DOE sites and national laboratories with the annual sustainability reporting process. Representatives from the SPO will be available on the call to answer specific questions with respect to Site Sustainability Plans (SSP) and the Consolidated Energy Data Report (CEDR).

  8. High temperature lined conduits, elbows and tees

    DOE Patents [OSTI]

    De Feo, Angelo (Passaic, NJ); Drewniany, Edward (Bergen, NJ)

    1982-01-01

    A high temperature lined conduit comprising, a liner, a flexible insulating refractory blanket around and in contact with the liner, a pipe member around the blanket and spaced therefrom, and castable rigid refractory material between the pipe member and the blanket. Anchors are connected to the inside diameter of the pipe and extend into the castable material. The liner includes male and female slip joint ends for permitting thermal expansion of the liner with respect to the castable material and the pipe member. Elbows and tees of the lined conduit comprise an elbow liner wrapped with insulating refractory blanket material around which is disposed a spaced elbow pipe member with castable refractory material between the blanket material and the elbow pipe member. A reinforcing band is connected to the elbow liner at an intermediate location thereon from which extend a plurality of hollow tubes or pins which extend into the castable material to anchor the lined elbow and permit thermal expansion. A method of fabricating the high temperature lined conduit, elbows and tees is also disclosed which utilizes a polyethylene layer over the refractory blanket after it has been compressed to maintain the refractory blanket in a compressed condition until the castable material is in place. Hot gases are then directed through the interior of the liner for evaporating the polyethylene and setting the castable material which permits the compressed blanket to come into close contact with the castable material.

  9. V-172: ISC BIND RUNTIME_CHECK Error Lets Remote Users Deny Service...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ISC BIND RUNTIMECHECK Error Lets Remote Users Deny Service Against Recursive Resolvers V-172: ISC BIND RUNTIMECHECK Error Lets Remote Users Deny Service Against Recursive...

  10. Structure and Ca[superscript 2+]-Binding Properties of the Tandem...

    Office of Scientific and Technical Information (OSTI)

    and Casuperscript 2+-Binding Properties of the Tandem Csubscript 2 Domains of E-Syt2 Citation Details In-Document Search Title: Structure and Casuperscript 2+-Binding ...

  11. Research Highlight

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    as indicated, and the Clausius-Clapeyron curves for liquid saturation are shown by the grey dashed lines. Arctic climate is changing at a rate exceeding that of other regions....

  12. Stockpile Stewardship Q

    National Nuclear Security Administration (NNSA)

    ... concepts exist, all must bring the fusion fuel to an extreme high-energy-density state. ... liners imploded on the Z Facility. Green and red dashed lines indicate the liner's ...

  13. Microsoft PowerPoint - one line pdfs for 1222.pptx

    Broader source: Energy.gov (indexed) [DOE]

    Preliminary One-Line Drawings Future Optima Substation Modified Shelby Substation

  14. Rectangular Dielectric-lined Accelerator Structure

    SciTech Connect (OSTI)

    Wang Changbiao; Yakovlev, V.P.; Hirshfield, J.L.

    2004-12-07

    Results are reported from analysis of a rectangular X-band dielectric-lined accelerator structure operating in the symmetric LSM-11 mode that has a number of favorable attributes, by comparison with dielectric-lined cylindrical structures. These attributes include use of two planar one-piece precision-ground TiN coated dielectric slabs free of joints, open slots along two opposing metallic faces to suppress all anti-symmetric higher-order modes and to facilitate high-speed pumping, and significant reduction of wall losses by use of evanescent vacuum gaps beyond the dielectric slabs. It is shown that a structure operating at 11.424 GHz can be built with a shunt impedance > 60 M{omega}/m using low-loss alumina as the dielectric.

  15. In-line real time air monitor

    DOE Patents [OSTI]

    Wise, Marcus B. (Kingston, TN); Thompson, Cyril V. (Knoxville, TN)

    1998-01-01

    An in-line gas monitor capable of accurate gas composition analysis in a continuous real time manner even under strong applied vacuum conditions operates by mixing an air sample with helium forming a sample gas in two complementary sample loops embedded in a manifold which includes two pairs of 3-way solenoid valves. The sample gas is then analyzed in an ion trap mass spectrometer on a continuous basis. Two valve drivers actuate the two pairs of 3-way valves in a reciprocating fashion, so that there is always flow through the in-line gas monitor via one or the other of the sample loops. The duty cycle for the two pairs of 3-way valves is varied by tuning the two valve drivers to a duty cycle typically between 0.2 to 0.7 seconds.

  16. In-line real time air monitor

    DOE Patents [OSTI]

    Wise, M.B.; Thompson, C.V.

    1998-07-14

    An in-line gas monitor capable of accurate gas composition analysis in a continuous real time manner even under strong applied vacuum conditions operates by mixing an air sample with helium forming a sample gas in two complementary sample loops embedded in a manifold which includes two pairs of 3-way solenoid valves. The sample gas is then analyzed in an ion trap mass spectrometer on a continuous basis. Two valve drivers actuate the two pairs of 3-way valves in a reciprocating fashion, so that there is always flow through the in-line gas monitor via one or the other of the sample loops. The duty cycle for the two pairs of 3-way valves is varied by tuning the two valve drivers to a duty cycle typically between 0.2 to 0.7 seconds. 3 figs.

  17. Line-of-sight deposition method

    DOE Patents [OSTI]

    Patten, J.W.; McClanahan, E.D.; Bayne, M.A.

    1980-04-16

    A line-of-sight method of depositing a film having substantially 100% of theoretical density on a substrate. A pressure vessel contains a target source having a surface thereof capable of emitting particles therefrom and a substrate with the source surface and the substrate surface positioned such that the source surface is substantially parallel to the direction of the particles impinging upon the substrate surface, the distance between the most remote portion of the substrate surface receiving the particles and the source surface emitting the particles in a direction parallel to the substrate surface being relatively small. The pressure in the vessel is maintained less than about 5 microns to prevent scattering and permit line-of-sight deposition. By this method the angles of incidence of the particles impinging upon the substrate surface are in the range of from about 45/sup 0/ to 90/sup 0/ even when the target surface area is greatly expanded to increase the deposition rate.

  18. X-ray line polarization spectroscopy of Li-like satellite line...

    Office of Scientific and Technical Information (OSTI)

    properties of satellite lines in Li-like Si driven by subpicosecond-duration laser pulses. ... Resource Relation: Journal Name: Journal of Physics B Research Org: Los Alamos National ...

  19. Strain monitoring averts line failure in Rockies

    SciTech Connect (OSTI)

    Miller, B.; Bukovansky, M.

    1987-08-10

    The case history of a landslide in the U.S. Rocky Mountains shows that the potential for pipeline monitoring in geologically sensitive areas, those subject to landslides and subsidence, for example. A properly installed monitoring system monitored by the pipeline operator, Western Gas Supply Co. (West Gas), Denver, provided an early warning of increasing line strains. The problem was complicated by rugged topography which is described here. Stability analysis was the key technique utilized in the process.

  20. Emergency Management Lines of Inquiry, April 2008

    Broader source: Energy.gov (indexed) [DOE]

    23, 2008 EMERGENCY MANAGEMENT Inspection Criteria, Activities, and Lines of Inquiry Functional Area: Hazards Survey (HS) and Emergency Planning Hazards Assessment (EPHA) Inspection Criteria: Emergency planning includes the identification of hazards and threats. A hazards survey is the qualitative portion of the hazards identification process. It briefly describes the potential impacts of emergency events or conditions and summarizes the planning and preparedness requirements that apply. If

  1. Heavy rains hamper Louisiana gas line

    SciTech Connect (OSTI)

    Horner, C.

    1983-06-01

    Despite heavy rains and flooding a 36-mile gas pipeline loop for Transcontinental Gas Pipe Line Corp. was completed from north of Starks (at the end of Transco's south Louisiana lateral) to the Lake Charles area. Somastic-coated, 42-in. grade X-60 pipe comprises 90% of the route. The contract included multiple 30-42 in. fabrications, installation of six 42-in. gate valves, and expansion of the Gillis compressor station.

  2. Gas lines chasing huge northeastern market

    SciTech Connect (OSTI)

    Watts, J.

    1982-03-01

    Gas for the Northeastern US market is the driving force behind three proposed projects to bring Canadian gas to the New England-New York area: the 360-mile New England States pipeline (Algonquin Gas Transmission Co., Transcontinental Gas Pipe Line Corp., Texas Eastern Transmission Corp., and Nova, an Alberta Corp.); the 261-mile Boundary Gas project (with Boundary Gas Inc., a consortium of 14 gas utilities with Tennessee Gas Pipeline Co. providing transportation); and the 158-mile Niagara pipeline (Transcontinental Gas Pipe Line Corp.). Although none has yet received government (US and Canadian) approval, at least one project - the New England States line - is expected to be operational by 1984, bringing 305 million CF of natural gas daily for US residential and industrial markets. Both countries stand to benefit from the three projects. For Canada, the sale of gas to New England provides a steady market for massive quantities of gas makes building a pipeline from gas-rich Alberta (that will also serve eastern Canada) economically feasible, and ensures the existence of a transportation network in the Maritime provinces for use when production begins off Newfoundland and Nova Scotia. For the US, the gas from Canada will help reduce the nation's dependence on foreign oil and provide additional supplies during the peakload winter season.

  3. Molecular dynamics investigation of the substrate binding mechanism in carboxylesterase

    SciTech Connect (OSTI)

    Chen, Qi; Luan, Zheng-Jiao; Cheng, Xiaolin; Xu, Jian-he

    2015-01-01

    A recombinant carboxylesterase, cloned from Pseudomonas putida and designated as rPPE, is capable of catalyzing the bioresolution of racemic 2-acetoxy-2-(2 -chlorophenyl)acetate (rac-AcO-CPA) with excellent (S)-enantioselectivity. Semi-rational design of the enzyme showed that the W187H variant could increase the activity by ~100-fold compared to the wild type (WT) enzyme. In this study, we performed all-atom molecular dynamics (MD) simulations of both apo-rPPE and rPPE in complex with (S)-AcO-CPA to gain insights into the origin of the increased catalysis in the W187H mutant. Our results show differential binding of (S)-AcO-CPA in the WT and W187H enzymes, especially the interactions of the substrate with the two active site residues Ser159 and His286. The replacement of Trp187 by His leads to considerable structural rearrangement in the active site of W187H. Unlike in the WT rPPE, the cap domain in the W187 mutant shows an open conformation in the simulations of both apo and substrate-bound enzymes. This open conformation exposes the catalytic triad to the solvent through a water accessible channel, which may facilitate the entry of the substrate and/or the exit of the product. Binding free energy calculations confirmed that the substrate binds more strongly in W187H than in WT. Based on these computational results, we further predicted that the mutations W187Y and D287G might also be able to increase the substrate binding, thus improve the enzyme s catalytic efficiency. Experimental binding and kinetic assays on W187Y and D287G show improved catalytic efficiency over WT, but not W187H. Contrary to our prediction, W187Y shows slightly decreased substrate binding coupled with a 100 fold increase in turn-over rate, while in D287G the substrate binding is 8 times stronger but with a slightly reduced turn-over rate. Our work provides important molecular-level insights into the binding of the (S)-AcO-CPA substrate to carboxylesterase rPPEs, which will help guide future development of more efficient rPPE variants.

  4. Orientation-dependent binding energy of graphene on palladium

    SciTech Connect (OSTI)

    Kappes, Branden B.; Ebnonnasir, Abbas; Ciobanu, Cristian V. [Department of Mechanical Engineering and Materials Science Program, Colorado School of Mines, Golden, Colorado 80401 (United States)] [Department of Mechanical Engineering and Materials Science Program, Colorado School of Mines, Golden, Colorado 80401 (United States); Kodambaka, Suneel [Department of Materials Science and Engineering, University of California, Los Angeles, Los Angeles, California 90095 (United States)] [Department of Materials Science and Engineering, University of California, Los Angeles, Los Angeles, California 90095 (United States)

    2013-02-04

    Using density functional theory calculations, we show that the binding strength of a graphene monolayer on Pd(111) can vary between physisorption and chemisorption depending on its orientation. By studying the interfacial charge transfer, we have identified a specific four-atom carbon cluster that is responsible for the local bonding of graphene to Pd(111). The areal density of such clusters varies with the in-plane orientation of graphene, causing the binding energy to change accordingly. Similar investigations can also apply to other metal substrates and suggests that physical, chemical, and mechanical properties of graphene may be controlled by changing its orientation.

  5. Stand-off transmission lines and method for making same

    DOE Patents [OSTI]

    Tuckerman, David B. (Livermore, CA)

    1991-01-01

    Standoff transmission lines in an integrated circuit structure are formed by etching away or removing the portion of the dielectric layer separating the microstrip metal lines and the ground plane from the regions that are not under the lines. The microstrip lines can be fabricated by a subtractive process of etching a metal layer, an additive process of direct laser writing fine lines followed by plating up the lines or a subtractive/additive process in which a trench is etched over a nucleation layer and the wire is electrolytically deposited. Microstrip lines supported on freestanding posts of dielectric material surrounded by air gaps are produced. The average dielectric constant between the lines and ground plane is reduced, resulting in higher characteristic impedance, less crosstalk between lines, increased signal propagation velocities, and reduced wafer stress.

  6. Stand-off transmission lines and method for making same

    DOE Patents [OSTI]

    Tuckerman, D.B.

    1991-05-21

    Standoff transmission lines in an integrated circuit structure are formed by etching away or removing the portion of the dielectric layer separating the microstrip metal lines and the ground plane from the regions that are not under the lines. The microstrip lines can be fabricated by a subtractive process of etching a metal layer, an additive process of direct laser writing fine lines followed by plating up the lines or a subtractive/additive process in which a trench is etched over a nucleation layer and the wire is electrolytically deposited. Microstrip lines supported on freestanding posts of dielectric material surrounded by air gaps are produced. The average dielectric constant between the lines and ground plane is reduced, resulting in higher characteristic impedance, less crosstalk between lines, increased signal propagation velocities, and reduced wafer stress. 16 figures.

  7. Fluid properties determine flow line blockage potential

    SciTech Connect (OSTI)

    Hunt, A.

    1996-07-15

    A thorough understanding of fluid properties helps in determining the potential of hydrates, paraffins, or asphaltenes to block subsea flow lines. Thermal, chemical, and mechanical methods are the main ways for preventing deposition. Already in both the North Sea and the Gulf of Mexico, blockages have led to significant losses in production and reserves recovery. This first article in a two-part series discusses thermal and chemical methods in overcoming fluid behavior problems caused by hydrate and other fluid constituents in subsea multiphase flow. The paper discusses subsea production, possible problems, nucleation, growth, deposition, preventing deposition, hydrate predictions, multiphase flow, and hydrate inhibition.

  8. SunLine Transit Agency | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Technology Validation » SunLine Transit Agency SunLine Transit Agency SunLine Transit Agency logo SunLine Transit Agency provides public transit and community services to California's Coachella Valley. The service area is more than 1,100 square miles and includes nine member cities, as well as Riverside County. Over the years, SunLine has pursued an aggressive strategy for implementing clean technologies into its fleet. SunLine began by switching its fleet to compressed natural gas (CNG), then

  9. Methods of use of cellulose binding domain proteins

    DOE Patents [OSTI]

    Shoseyov, O.; Shpiegl, I.; Goldstein, M.A.; Doi, R.H.

    1997-09-23

    A cellulose binding domain (CBD) having a high affinity for crystalline cellulose and chitin is disclosed, along with methods for the molecular cloning and recombinant production. Fusion products comprising the CBD and a second protein are likewise described. A wide range of applications are contemplated for both the CBD and the fusion products, including drug delivery, affinity separations, and diagnostic techniques. 16 figs.

  10. A probabilistic approach to microRNA-target binding

    SciTech Connect (OSTI)

    Ogul, Hasan; Umu, Sinan U.; Bioinformatics Program, Informatics Institute, Middle East Technical University, Cankaya TR-06800, Ankara ; Tuncel, Y. Yener; Akkaya, Mahinur S.

    2011-09-16

    Highlights: {yields} A new probabilistic model is introduced for microRNA-target binding. {yields} The new model significantly outperforms RNAHybrid and miRTif. {yields} The experiments can unveil the effects of the type and directions of distinct base pairings. -- Abstract: Elucidation of microRNA activity is a crucial step in understanding gene regulation. One key problem in this effort is how to model the pairwise interactions of microRNAs with their targets. As this interaction is strongly mediated by their sequences, it is desired to set-up a probabilistic model to explain the binding preferences between a microRNA sequence and the sequence of a putative target. To this end, we introduce a new model of microRNA-target binding, which transforms an aligned duplex to a new sequence and defines the likelihood of this sequence using a Variable Length Markov Chain. It offers a complementary representation of microRNA-mRNA pairs for microRNA target prediction tools or other probabilistic frameworks of integrative gene regulation analysis. The performance of present model is evaluated by its ability to predict microRNA-target mRNA interaction given a mature microRNA sequence and a putative mRNA binding site. In regard to classification accuracy, it outperforms two recent methods based on thermodynamic stability and sequence complementarity. The experiments can also unveil the effects of base pairing types and non-seed region in duplex formation.

  11. Methods of use of cellulose binding domain proteins

    DOE Patents [OSTI]

    Shoseyov, Oded (Karmey Yosef, IL); Shpiegl, Itai (Rehovot, IL); Goldstein, Marc A. (Davis, CA); Doi, Roy H. (Davis, CA)

    1997-01-01

    A cellulose binding domain (CBD) having a high affinity for crystalline cellulose and chitin is disclosed, along with methods for the molecular cloning and recombinant production thereof. Fusion products comprising the CBD and a second protein are likewise described. A wide range of applications are contemplated for both the CBD and the fusion products, including drug delivery, affinity separations, and diagnostic techniques.

  12. Methods of detection using a cellulose binding domain fusion product

    DOE Patents [OSTI]

    Shoseyov, Oded (Shimshon, IL); Shpiegl, Itai (North Gallilea, IL); Goldstein, Marc A. (Davis, CA); Doi, Roy H. (Davis, CA)

    1999-01-01

    A cellulose binding domain (CBD) having a high affinity for crystalline cellulose and chitin is disclosed, along with methods for the molecular cloning and recombinant production thereof. Fusion products comprising the CBD and a second protein are likewise described. A wide range of applications are contemplated for both the CBD and the fusion products, including drug delivery, affinity separations, and diagnostic techniques.

  13. Inhibition of cell-cell binding by lipid assemblies

    DOE Patents [OSTI]

    Nagy, Jon O. (Rodeo, CA); Bargatze, Robert F. (Bozeman, MT)

    2001-05-22

    This invention relates generally to the field of therapeutic compounds designed to interfere between the binding of ligands and their receptors on cell surface. More specifically, it provides products and methods for inhibiting cell migration and activation using lipid assemblies with surface recognition elements that are specific for the receptors involved in cell migration and activation.

  14. Methods of detection using a cellulose binding domain fusion product

    DOE Patents [OSTI]

    Shoseyov, O.; Shpiegl, I.; Goldstein, M.A.; Doi, R.H.

    1999-01-05

    A cellulose binding domain (CBD) having a high affinity for crystalline cellulose and chitin is disclosed, along with methods for the molecular cloning and recombinant production. Fusion products comprising the CBD and a second protein are likewise described. A wide range of applications are contemplated for both the CBD and the fusion products, including drug delivery, affinity separations, and diagnostic techniques. 34 figs.

  15. Inhibition Of Call-Cell Binding By Kipid Assemblies

    DOE Patents [OSTI]

    Nagy, Jon O. (Rodeo, CA), Bargatze, Robert F. (Bozeman, MT)

    2003-12-16

    This invention relates generally to the field of therapeutic compounds designed to interfere between the binding of ligands and their receptors on cell surface. More specifically, it provides products and methods for inhibiting cell migration and activation using lipid assemblies with surface recognition elements that are specific for the receptors involved in cell migration and activation.

  16. Workshop on gate valve pressure locking and thermal binding

    SciTech Connect (OSTI)

    Brown, E.J.

    1995-07-01

    The purpose of the Workshop on Gate Valve Pressure Locking and Thermal Binding was to discuss pressure locking and thermal binding issues that could lead to inoperable gate valves in both boiling water and pressurized water reactors. The goal was to foster exchange of information to develop the technical bases to understand the phenomena, identify the components that are susceptible, discuss actual events, discuss the safety significance, and illustrate known corrective actions that can prevent or limit the occurrence of pressure locking or thermal binding. The presentations were structured to cover U.S. Nuclear Regulatory Commission staff evaluation of operating experience and planned regulatory activity; industry discussions of specific events, including foreign experience, and efforts to determine causes and alleviate the affects; and valve vendor experience and recommended corrective action. The discussions indicated that identifying valves susceptible to pressure locking and thermal binding was a complex process involving knowledge of components, systems, and plant operations. The corrective action options are varied and straightforward.

  17. On-line subsea multiphase flow measurement

    SciTech Connect (OSTI)

    High, G.; Frantzen, K.H.; Marshall, M.

    1995-12-31

    This paper describes the final detailed design, engineering, and installation phase of a Joint Industry Program to qualify a robust subsea multiphase flowmeter module for long-term installation on a North Sea manifold tie-in. Multiphase subsea production has become a common method of hydrocarbon recovery in all areas of offshore E and P. In the North Sea, many developments are subsea satellites with multiphase well-fluids being comingled prior to processing. The system described meets this challenge by offering a cost effective solution to real-time well monitoring as an alternative to the conventional test separator, removing the need for test lines and shutting in wells for testing. The multiphase instrument allows on-line well fluid analysis, and is also an important tool for reservoir management and field analysis, and provides a means of implementing field allocation metering thereby simplifying small marginal field developments. This project is one of the first subsea multiphase flowmeter installations engineered for long-term subsea service, and designed as an integrated component of the subsea production control system.

  18. EIS-0465: Mid-Atlantic Power Pathway Transmission Line Project...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    5: Mid-Atlantic Power Pathway Transmission Line Project in Maryland and Delaware EIS-0465: Mid-Atlantic Power Pathway Transmission Line Project in Maryland and Delaware March 4, ...

  19. Plains & Eastern Clean Line Project Proposal for New or Upgraded...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Project Proposal for New or Upgraded Transmission Line Projects Under Section 1222 of the Energy Policy Act of 2005 Plains & Eastern Clean Line Project Proposal for New or Upgraded ...

  20. Power Line Plan of Development Outline | Open Energy Information

    Open Energy Info (EERE)

    Line Plan of Development Outline Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- OtherOther: Power Line Plan of Development OutlineLegal...

  1. EIS-0421: Big Eddy-Knight Transmission Line

    Broader source: Energy.gov [DOE]

    BPA is proposing to build a new 500 kilovolt (kV) transmission line in Wasco County, Oregon and Klickitat County, Washington and a new substation in Klickitat County. The new BPA transmission line...

  2. Updated Web Tool Focuses on Bottom Line for Distributed Wind...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Updated Web Tool Focuses on Bottom Line for Distributed Wind Turbines Updated Web Tool Focuses on Bottom Line for Distributed Wind Turbines January 10, 2013 - 2:43pm Addthis This...

  3. SSRL Beam Lines by Technique | Stanford Synchrotron Radiation...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Beam Lines by Number | SPEAR3 Parameters Supported Technique(s) Beam Line X-ray Absorption Spectroscopy Biological x-ray absorption spectroscopy 2-2, 4-3, 7-3, 9-3, 14-3...

  4. File:08HIATransmissionLineApproval.pdf | Open Energy Information

    Open Energy Info (EERE)

    8HIATransmissionLineApproval.pdf Jump to: navigation, search File File history File usage Metadata File:08HIATransmissionLineApproval.pdf Size of this preview: 463 599 pixels....

  5. Titanium and germanium lined hohlraums and halfraums as multi...

    Office of Scientific and Technical Information (OSTI)

    Titanium and germanium lined hohlraums and halfraums as multi-keV X-ray radiators Citation Details In-Document Search Title: Titanium and germanium lined hohlraums and halfraums as...

  6. Mitsui O S K Lines Ltd | Open Energy Information

    Open Energy Info (EERE)

    O S K Lines Ltd Jump to: navigation, search Name: Mitsui O.S.K. Lines Ltd Place: Tokyo, Japan Zip: 1058688 Sector: Services Product: A shipping company, with services including...

  7. Insulate Steam Distribution and Condensate Return Lines | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Insulate Steam Distribution and Condensate Return Lines Insulate Steam Distribution and Condensate Return Lines This tip sheet on insulating steam distribution and condensate return lines provides how-to advice for improving industrial steam systems using low-cost, proven practices and technologies. STEAM TIP SHEET #2 PDF icon Insulate Steam Distribution and Condensate Return Lines (January 2012) More Documents & Publications Use a Vent Condenser to Recover Flash Steam Energy

  8. Advance Funding and Development Agreement: Plains & Eastern Clean Line

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Transmission Project (September 20, 2012) | Department of Energy Advance Funding and Development Agreement: Plains & Eastern Clean Line Transmission Project (September 20, 2012) Advance Funding and Development Agreement: Plains & Eastern Clean Line Transmission Project (September 20, 2012) PDF icon Advance Funding and Development Agreement: Plains & Eastern Clean Line Transmission Project (September 20, 2012) More Documents & Publications Plains and Eastern Clean Line

  9. Appendix B: Rules and Directives Applicable to Nuclear Facilities Line

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Management Oversight | Department of Energy Appendix B: Rules and Directives Applicable to Nuclear Facilities Line Management Oversight Appendix B: Rules and Directives Applicable to Nuclear Facilities Line Management Oversight Appendix B to DOE G 226.1-2A "Federal Line Management Oversight of Department of Energy Nuclear Facilities". Consists of a list of rules and directives that are applicable to nuclear facilities line management oversight. PDF icon Appendix B: Rules and

  10. Energy Jobs: Utility Line Worker | Department of Energy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Utility Line Worker Energy Jobs: Utility Line Worker November 19, 2014 - 10:07am Addthis Line workers get hands-on experience with an electrical pole as part of their training. | Photo courtesy of David Weaver. Line workers get hands-on experience with an electrical pole as part of their training. | Photo courtesy of David Weaver. Allison Lantero Allison Lantero Digital Content Specialist, Office of Public Affairs How can I participate? Check out our Infographic on Understanding the Grid. Send

  11. Energy Jobs: Utility Line Worker | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Jobs: Utility Line Worker Energy Jobs: Utility Line Worker November 19, 2014 - 10:07am Addthis Line workers get hands-on experience with an electrical pole as part of their training. | Photo courtesy of David Weaver. Line workers get hands-on experience with an electrical pole as part of their training. | Photo courtesy of David Weaver. Allison Lantero Allison Lantero Digital Content Specialist, Office of Public Affairs How can I participate? Check out our Infographic on Understanding the

  12. Green Power Transmission Line Given New Life | Department of Energy

    Office of Environmental Management (EM)

    Power Transmission Line Given New Life Green Power Transmission Line Given New Life March 11, 2010 - 4:34pm Addthis Poles for the Montana-Alberta Tie Line are unloaded outside Shelby, Mont. | Photo courtesy of Tonbridge Power Poles for the Montana-Alberta Tie Line are unloaded outside Shelby, Mont. | Photo courtesy of Tonbridge Power Stephen Graff Former Writer & editor for Energy Empowers, EERE Thanks to funds from the American Recovery and Reinvestment Act, construction of a green power

  13. On-line process control monitoring system

    DOE Patents [OSTI]

    O'Rourke, Patrick E. (Martinez, GA); Van Hare, David R. (Aiken, SC); Prather, William S. (Augusta, GA)

    1992-01-01

    An on-line, fiber-optic based apparatus for monitoring the concentration of a chemical substance at a plurality of locations in a chemical processing system comprises a plurality of probes, each of which is at a different location in the system, a light source, optic fibers for carrying light to and from the probes, a multiplexer for switching light from the source from one probe to the next in series, a diode array spectrophotometer for producing a spectrum from the light received from the probes, and a computer programmed to analyze the spectra so produced. The probes allow the light to pass through the chemical substance so that a portion of the light is absorbed before being returned to the multiplexer. A standard and a reference cell are included for data validation and error checking.

  14. On-line tritium production monitor

    DOE Patents [OSTI]

    Mihalczo, John T. (Oak Ridge, TN)

    1993-01-01

    A scintillation optical fiber system for the on-line monitoring of nuclear reactions in an event-by-event manner is described. In the measurement of tritium production one or more optical fibers are coated with enriched .sup.6 Li and connected to standard scintillation counter circuitry. A neutron generated .sup.6 Li(n )T reaction occurs in the coated surface of .sup.6 Li-coated fiber to produce energetic alpha and triton particles one of which enters the optical fiber and scintillates light through the fiber to the counting circuit. The coated optical fibers can be provided with position sensitivity by placing a mirror at the free end of the fibers or by using pulse counting circuits at both ends of the fibers.

  15. Spectral line broadening in magnetized black holes

    SciTech Connect (OSTI)

    Frolov, Valeri P.; Shoom, Andrey A.; Tzounis, Christos E-mail: ashoom@ualberta.ca

    2014-07-01

    We consider weakly magnetized non-rotating black holes. In the presence of a regular magnetic field the motion of charged particles in the vicinity of a black hole is modified. As a result, the position of the innermost stable circular orbit (ISCO) becomes closer to the horizon. When the Lorentz force is repulsive (directed from the black hole) the ISCO radius can reach the gravitational radius. In the process of accretion charged particles (ions) of the accreting matter can be accumulated near their ISCO, while neutral particles fall down to the black hole after they reach 6M radius. The sharp spectral line Fe α, emitted by iron ions at such orbits, is broadened when the emission is registered by a distant observer. In this paper we study this broadening effect and discuss how one can extract information concerning the strength of the magnetic field from the observed spectrum.

  16. Atomic line emission analyzer for hydrogen isotopes

    DOE Patents [OSTI]

    Kronberg, James W. (108 Independent Blvd., Aiken, SC 29801)

    1993-01-01

    Apparatus for isotopic analysis of hydrogen comprises a low pressure chamber into which a sample of hydrogen is introduced and then exposed to an electrical discharge to excite the electrons of the hydrogen atoms to higher energy states and thereby cause the emission of light on the return to lower energy states, a Fresnel prism made at least in part of a material anomalously dispersive to the wavelengths of interest for dispersing the emitted light, and a photodiode array for receiving the dispersed light. The light emitted by the sample is filtered to pass only the desired wavelengths, such as one of the lines of the Balmer series for hydrogen, the wavelengths of which differ slightly from one isotope to another. The output of the photodiode array is processed to determine the relative amounts of each isotope present in the sample. Additionally, the sample itself may be recovered using a metal hydride.

  17. Dispersion-free radial transmission lines

    DOE Patents [OSTI]

    Caporaso, George J. (Livermore, CA); Nelson, Scott D. (Patterson, CA)

    2011-04-12

    A dispersion-free radial transmission line ("DFRTL") preferably for linear accelerators, having two plane conductors each with a central hole, and an electromagnetically permeable material ("EPM") between the two conductors and surrounding a channel connecting the two holes. At least one of the material parameters of relative magnetic permeability, relative dielectric permittivity, and axial width of the EPM is varied as a function of radius, so that the characteristic impedance of the DFRTL is held substantially constant, and pulse transmission therethrough is substantially dispersion-free. Preferably, the EPM is divided into concentric radial sections, with the varied material parameters held constant in each respective section but stepwise varied between sections as a step function of the radius. The radial widths of the concentric sections are selected so that pulse traversal time across each section is the same, and the varied material parameters of the concentric sections are selected to minimize traversal error.

  18. On-line tritium production monitor

    DOE Patents [OSTI]

    Mihalczo, J.T.

    1993-11-23

    A scintillation optical fiber system for the on-line monitoring of nuclear reactions in an event-by-event manner is described. In the measurement of tritium production one or more optical fibers are coated with enriched {sup 6}Li and connected to standard scintillation counter circuitry. A neutron generated {sup 6}Li(n)T reaction occurs in the coated surface of {sup 6}Li-coated fiber to produce energetic alpha and triton particles one of which enters the optical fiber and scintillates light through the fiber to the counting circuit. The coated optical fibers can be provided with position sensitivity by placing a mirror at the free end of the fibers or by using pulse counting circuits at both ends of the fibers. 5 figures.

  19. Second Line of Defense Spares Program Assessment

    SciTech Connect (OSTI)

    Henderson, Dale L.; Muller, George; Mercier, Theresa M.; Brigantic, Robert T.; Perkins, Casey J.; Cooley, Scott K.

    2012-11-20

    The Office of the Second Line of Defense (SLD) is part of the Department of Energy‘s (DOE) National Nuclear Security Administration (NNSA). The SLD Program accomplishes its critical global security mission by forming cooperative relationships with partner countries to install passive radiation detection systems that augment traditional inspection and law enforcement measures by alerting border officials to the presence of special nuclear or other radiological materials in cross-border traffic. An important tenet of the program is to work collaboratively with these countries to establish the necessary processes, procedures, infrastructure and conditions that will enable them to fully assume the financial and technical responsibilities for operating the equipment. As the number of operational deployments grows, the SLD Program faces an increasingly complex logistics process to promote the timely and efficient supply of spare parts.

  20. Atomic line emission analyzer for hydrogen isotopes

    DOE Patents [OSTI]

    Kronberg, J.W.

    1993-03-30

    Apparatus for isotopic analysis of hydrogen comprises a low pressure chamber into which a sample of hydrogen is introduced and then exposed to an electrical discharge to excite the electrons of the hydrogen atoms to higher energy states and thereby cause the emission of light on the return to lower energy states, a Fresnel prism made at least in part of a material anomalously dispersive to the wavelengths of interest for dispersing the emitted light, and a photodiode array for receiving the dispersed light. The light emitted by the sample is filtered to pass only the desired wavelengths, such as one of the lines of the Balmer series for hydrogen, the wavelengths of which differ slightly from one isotope to another. The output of the photodiode array is processed to determine the relative amounts of each isotope present in the sample. Additionally, the sample itself may be recovered using a metal hydride.

  1. Atomic line emission analyzer for hydrogen isotopes

    DOE Patents [OSTI]

    Kronberg, J.W.

    1991-05-08

    Apparatus for isotopic analysis of hydrogen comprises a low pressure chamber into which a sample of hydrogen is introduced and then exposed to an electrical discharge to excite the electrons of the hydrogen atoms to higher energy states and thereby cause the emission of light on the return to lower energy states, a Fresnel prism made at least in part of a material anomalously dispersive to the wavelengths of interest for dispersing the emitted light, and a photodiode array for receiving the dispersed light. The light emitted by the sample is filtered to pass only the desired wavelengths, such as one of the lines of the Balmer series for hydrogen, the wavelengths of which differ slightly from one isotope to another. The output of the photodiode array is processed to determine the relative amounts of each isotope present in the sample. Additionally, the sample itself may be recovered using, a metal hydride.

  2. Method for bonding a transmission line to a downhole tool

    DOE Patents [OSTI]

    Hall, David R. (Provo, UT); Fox, Joe (Spanish Fork, UT)

    2007-11-06

    An apparatus for bonding a transmission line to the central bore of a downhole tool includes a pre-formed interface for bonding a transmission line to the inside diameter of a downhole tool. The pre-formed interface includes a first surface that substantially conforms to the outside contour of a transmission line and a second surface that substantially conforms to the inside diameter of a downhole tool. In another aspect of the invention, a method for bonding a transmission line to the inside diameter of a downhole tool includes positioning a transmission line near the inside wall of a downhole tool and placing a mold near the transmission line and the inside wall. The method further includes injecting a bonding material into the mold and curing the bonding material such that the bonding material bonds the transmission line to the inside wall.

  3. Binding Behavior of Dopamine Transporter Key to Understanding Chemical

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Reactions in the Brain Binding Behavior of Dopamine Transporter Key to Understanding Chemical Reactions in the Brain Print Most people have heard of adrenaline, the chemical that causes the "fight or flight" reaction in humans. Most people have also heard of the chemical substances cocaine and methamphetamine, which also elicit a particular (perhaps desired) human response. What most people do not know is that the same receptors in the human brain recognize the natural, or

  4. Binding Behavior of Dopamine Transporter Key to Understanding Chemical

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Reactions in the Brain Binding Behavior of Dopamine Transporter Key to Understanding Chemical Reactions in the Brain Print Most people have heard of adrenaline, the chemical that causes the "fight or flight" reaction in humans. Most people have also heard of the chemical substances cocaine and methamphetamine, which also elicit a particular (perhaps desired) human response. What most people do not know is that the same receptors in the human brain recognize the natural, or

  5. Binding Behavior of Dopamine Transporter Key to Understanding Chemical

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Reactions in the Brain Binding Behavior of Dopamine Transporter Key to Understanding Chemical Reactions in the Brain Print Most people have heard of adrenaline, the chemical that causes the "fight or flight" reaction in humans. Most people have also heard of the chemical substances cocaine and methamphetamine, which also elicit a particular (perhaps desired) human response. What most people do not know is that the same receptors in the human brain recognize the natural, or

  6. Reversible Acid Gas Capture Using CO2-Binding Organic Liquids

    SciTech Connect (OSTI)

    Heldebrant, David J.; Koech, Phillip K.; Yonker, Clement R.; Rainbolt, James E.; Zheng, Feng

    2010-08-31

    Acid gas scrubbing technology is predominantly aqueous alkanolamine based. Of the acid gases, CO2, H2S and SO2 have been shown to be reversible, however there are serious disadvantages with corrosion and high regeneration costs. The primary scrubbing system composed of monoethanolamine is limited to 30% by weight because of the highly corrosive solution. This gravimetric limitation limits the CO2 volumetric (?108 g/L) and gravimetric capacity (?7 wt%) of the system. Furthermore the scrubbing system has a large energy penalty from pumping and heating the excess water required to dissolve the MEA bicarbonate salt. Considering the high specific heat of water (4 j/g-1K-1), low capacities and the high corrosion we set out to design a fully organic solvent that can chemically bind all acid gases i.e. CO2 as reversible alkylcarbonate ionic liquids or analogues thereof. Having a liquid acid gas carrier improves process economics because there is no need for excess solvent to pump and to heat. We have demonstrated illustrated in Figure 1, that CO2-binding organic liquids (CO2BOLs) have a high CO2 solubility paired with a much lower specific heat (<1.5 J/g-1K-1) than aqueous systems. CO2BOLs are a subsection of a larger class of materials known as Binding Organic Liquids (BOLs). Our BOLs have been shown to reversibly bind and release COS, CS2, and SO2, which we denote COSBOLS, CS2BOLs and SO2BOLs. Our BOLs are highly tunable and can be designed for post or pre-combustion gas capture. The design and testing of the next generation zwitterionic CO2BOLs and SO2BOLs are presented.

  7. DNA-Binding Mechanism in Prokaryotic Partition Complex Formation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    DNA-Binding Mechanism in Prokaryotic Partition Complex Formation Print The faithful inheritance of genetic information, essential for all organisms, requires accurate movement and positioning of replicated DNA to daughter cells during cell division. In cells without distinct nuclei (prokaryotes), this process, called partition or segregation, is mediated by par systems. The prototype system of prokaryotic partition is the Escherichia coli P1 plasmid par system, which consists of a centromere

  8. Characterization of Selective Binding of Alkali Cations with Carboxylate

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Characterization of Selective Binding of Alkali Cations with Carboxylate Print During its lifetime, a cell spends a considerable fraction of its energy pumping sodium and calcium out and potassium in. This balancing process is similar to that found in the coils of the DNA double helix, where specific ions nestle and help stabilize this macromolecule. These are only two examples of selective ion interactions in biology; there are many others also vital to life. The existence of these interactions

  9. Characterization of Selective Binding of Alkali Cations with Carboxylate

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Characterization of Selective Binding of Alkali Cations with Carboxylate Print During its lifetime, a cell spends a considerable fraction of its energy pumping sodium and calcium out and potassium in. This balancing process is similar to that found in the coils of the DNA double helix, where specific ions nestle and help stabilize this macromolecule. These are only two examples of selective ion interactions in biology; there are many others also vital to life. The existence of these interactions

  10. Characterization of Selective Binding of Alkali Cations with Carboxylate

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Characterization of Selective Binding of Alkali Cations with Carboxylate Print During its lifetime, a cell spends a considerable fraction of its energy pumping sodium and calcium out and potassium in. This balancing process is similar to that found in the coils of the DNA double helix, where specific ions nestle and help stabilize this macromolecule. These are only two examples of selective ion interactions in biology; there are many others also vital to life. The existence of these interactions

  11. Binding Behavior of Dopamine Transporter Key to Understanding Chemical

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Reactions in the Brain Binding Behavior of Dopamine Transporter Key to Understanding Chemical Reactions in the Brain Print Most people have heard of adrenaline, the chemical that causes the "fight or flight" reaction in humans. Most people have also heard of the chemical substances cocaine and methamphetamine, which also elicit a particular (perhaps desired) human response. What most people do not know is that the same receptors in the human brain recognize the natural, or

  12. DNA-Binding Mechanism in Prokaryotic Partition Complex Formation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    DNA-Binding Mechanism in Prokaryotic Partition Complex Formation Print The faithful inheritance of genetic information, essential for all organisms, requires accurate movement and positioning of replicated DNA to daughter cells during cell division. In cells without distinct nuclei (prokaryotes), this process, called partition or segregation, is mediated by par systems. The prototype system of prokaryotic partition is the Escherichia coli P1 plasmid par system, which consists of a centromere

  13. Characterization of Selective Binding of Alkali Cations with Carboxylate

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Characterization of Selective Binding of Alkali Cations with Carboxylate Print During its lifetime, a cell spends a considerable fraction of its energy pumping sodium and calcium out and potassium in. This balancing process is similar to that found in the coils of the DNA double helix, where specific ions nestle and help stabilize this macromolecule. These are only two examples of selective ion interactions in biology; there are many others also vital to life. The existence of these interactions

  14. Characterization of Selective Binding of Alkali Cations with Carboxylate

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Characterization of Selective Binding of Alkali Cations with Carboxylate Print During its lifetime, a cell spends a considerable fraction of its energy pumping sodium and calcium out and potassium in. This balancing process is similar to that found in the coils of the DNA double helix, where specific ions nestle and help stabilize this macromolecule. These are only two examples of selective ion interactions in biology; there are many others also vital to life. The existence of these interactions

  15. Protein Immobilization in Metal-Organic Frameworks by Covalent Binding |

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Center for Gas SeparationsRelevant to Clean Energy Technologies | Blandine Jerome Protein Immobilization in Metal-Organic Frameworks by Covalent Binding Previous Next List Xuan Wang, Trevor A. Makal and Hong-Cai Zhou, Aust. J. Chem. 67, 1629-1631 (2014) DOI: 10.1071/CH14104 CH14104_TOC Abstract: Metal-organic frameworks (MOFs), possessing a well defined system of pores, demonstrate extensive potential serving as a platform in biological catalysis. Successful immobilization of enzymes in a

  16. High molecular weight polysaccharide that binds and inhibits virus

    DOE Patents [OSTI]

    Konowalchuk, Thomas W

    2014-01-14

    This invention provides a high molecular weight polysaccharide capable of binding to and inhibiting virus and related pharmaceutical formulations and methods on inhibiting viral infectivity and/or pathogenicity, as well as immunogenic compositions. The invention further methods of inhibiting the growth of cancer cells and of ameliorating a symptom of aging. Additionally, the invention provides methods of detecting and/or quantifying and/or isolating viruses.

  17. Gold Binding by Native and Chemically Modified Hops Biomasses

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    López, M. Laura; Gardea-Torresdey, J. L.; Peralta-Videa, J. R.; de la Rosa, G.; Armendáriz, V.; Herrera, I.; Troiani, H.; Henning, J.

    2005-01-01

    Heavy metals from mining, smelting operations and other industrial processing facilities pollute wastewaters worldwide. Extraction of metals from industrial effluents has been widely studied due to the economic advantages and the relative ease of technical implementation. Consequently, the search for new and improved methodologies for the recovery of gold has increased. In this particular research, the use of cone hops biomass ( Humulus lupulus ) was investigated as a new option for gold recovery. The results showed that the gold binding to native hops biomass was pH dependent from pH 2 to pH 6, with a maximum percentage bindingmore » at pH 3. Time dependency studies demonstrated that Au(III) binding to native and modified cone hops biomasses was found to be time independent at pH 2 while at pH 5, it was time dependent. Capacity experiments demonstrated that at pH 2, esterified hops biomass bound 33.4 mg Au/g of biomass, while native and hydrolyzed hops biomasses bound 28.2 and 12.0 mg Au/g of biomass, respectively. However, at pH 5 the binding capacities were 38.9, 37.8 and 11.4 mg of Au per gram of native, esterified and hydrolyzed hops biomasses, respectively.« less

  18. Two-photon Absorption In Quantum Dots,quantum Dashes And Related Materials

    SciTech Connect (OSTI)

    Jain, Ravinder

    2009-08-31

    We have proposed the use of USQDs for various deep-tissue biological imaging applications, notably wavelength-multiplexed multicolor imaging and intra-nuclear studies such as those involving cell apoptosis, and have studied the issue of maximizing two-photon absorption-induced fluorescence (TPAF) signals from CdSe/ZnS USQDs to be used for this application. In particular, using 2 nm USQDs, we have shown that the TPAF signal at 780 nm is ~ 8 times that at 850 nm and 68 times that at 900 nm, two wavelengths that have been used in previous studies using CdSe/ZnS SQDs for deep-tissue imaging of biological studies via TPAF .

  19. Demonstration of Datacenter Automation Software and Hardware (DASH) at the California Franchise Tax Board

    SciTech Connect (OSTI)

    Bell, Geoffrey C.; Federspiel, Clifford

    2009-12-18

    Control software and wireless sensors designed for closed-loop, monitoring and control of IT equipment's inlet air temperatures in datacenters were evaluated and tested while other datacenter cooling best practices were implemented. The controls software and hardware along with each best practice were installed sequentially and evaluated using a measurement and verification procedure between each measure. The results show that the overall project eliminates 475,239 kWh per year, which is 21.3percent of the baseline energy consumption of the data center. The total project, including the best practices will save $42,772 per year and cost $134,057 yielding a simple payback of 3.1 years. However, the control system alone eliminates 59.6percent of the baseline energy used to move air in the datacenter and 13.6percent of the baseline cooling energy, which is 15.2percent of the baseline energy consumption (see Project Approach, Task 1, below, for additional information) while keeping temperatures substantially within the limits recommended by ASHRAE. Savings attributed to the control system are $30,564 per year with a cost $56,824 for a simple payback of 1.9 years.

  20. V-058: Microsoft Internet Explorer CDwnBindInfo Object Reuse...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    8: Microsoft Internet Explorer CDwnBindInfo Object Reuse Flaw Lets Remote Users Execute Arbitrary Code V-058: Microsoft Internet Explorer CDwnBindInfo Object Reuse Flaw Lets Remote...

  1. New Insights into the RNA-Binding and E3 Ubiquitin Ligase Activities...

    Office of Scientific and Technical Information (OSTI)

    New Insights into the RNA-Binding and E3 Ubiquitin Ligase Activities of Roquins Citation Details In-Document Search Title: New Insights into the RNA-Binding and E3 Ubiquitin Ligase...

  2. Plasticity of the Quinone-binding Site of the Complex II Homolog...

    Office of Scientific and Technical Information (OSTI)

    Plasticity of the Quinone-binding Site of the Complex II Homolog Quinol:Fumarate Reductase Citation Details In-Document Search Title: Plasticity of the Quinone-binding Site of the...

  3. Discovery of a new ATP-binding motif involved in peptidic azoline...

    Office of Scientific and Technical Information (OSTI)

    Discovery of a new ATP-binding motif involved in peptidic azoline biosynthesis Citation Details In-Document Search Title: Discovery of a new ATP-binding motif involved in peptidic ...

  4. Discovery and Characterization of a Cell-Permeable, Small-Molecule c-Abl Kinase Activator that Binds to the Myristoyl Binding Site

    SciTech Connect (OSTI)

    Yang, Jingsong; Campobasso, Nino; Biju, Mangatt P.; Fisher, Kelly; Pan, Xiao-Qing; Cottom, Josh; Galbraith, Sarah; Ho, Thau; Zhang, Hong; Hong, Xuan; Ward, Paris; Hofmann, Glenn; Siegfried, Brett; Zappacosta, Francesca; Washio, Yoshiaki; Cao, Ping; Qu, Junya; Bertrand, Sophie; Wang, Da-Yuan; Head, Martha S.; Li, Hu; Moores, Sheri; Lai, Zhihong; Johanson, Kyung; Burton, George; Erickson-Miller, Connie; Simpson, Graham; Tummino, Peter; Copeland, Robert A.; Oliff, Allen

    2014-10-02

    c-Abl kinase activity is regulated by a unique mechanism involving the formation of an autoinhibited conformation in which the N-terminal myristoyl group binds intramolecularly to the myristoyl binding site on the kinase domain and induces the bending of the {alpha}I helix that creates a docking surface for the SH2 domain. Here, we report a small-molecule c-Abl activator, DPH, that displays potent enzymatic and cellular activity in stimulating c-Abl activation. Structural analyses indicate that DPH binds to the myristoyl binding site and prevents the formation of the bent conformation of the {alpha}I helix through steric hindrance, a mode of action distinct from the previously identified allosteric c-Abl inhibitor, GNF-2, that also binds to the myristoyl binding site. DPH represents the first cell-permeable, small-molecule tool compound for c-Abl activation.

  5. Reversible CO Binding in Metal-Organic Frameworks | Center for Gas

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    SeparationsRelevant to Clean Energy Technologies | Blandine Jerome Reversible CO Binding in Metal-Organic Frameworks

  6. Pipe line companies to install 14,766 miles of lines in 1994

    SciTech Connect (OSTI)

    Ives, G. Jr.

    1994-01-01

    This paper reviews the historical and projected pipeline construction activities in the US and around the world. It gives mileage values for all types of oil and gas transmission lines, both on and offshore. Tables provides information on the diameters of these pipelines and information on compressor stations planned and constructed. Known major construction projects are listed by company name with a brief description of the proposed project.

  7. Second Line of Defense Spares Program

    SciTech Connect (OSTI)

    Henderson, Dale L.; Holmes, Aimee E.; Muller, George; Mercier, Theresa M.; Brigantic, Robert T.; Perkins, Casey J.; Cooley, Scott K.; Thorsen, Darlene E.

    2012-11-20

    During Fiscal Year 2012, a team from the Pacific Northwest National Laboratory (PNNL) conducted an assessment and analysis of the Second Line of Defense (SLD) Sustainability spare parts program. Spare parts management touches many aspects of the SLD Sustainability Program including contracting and integration of Local Maintenance Providers (LMP), equipment vendors, analyses and metrics on program performance, system state of health, and maintenance practices. Standardized spares management will provide better data for decisions during site transition phase and will facilitate transition to host country sustainability ownership. The effort was coordinated with related SLD Sustainability Program initiatives, including a configuration items baselining initiative, a metrics initiative, and a maintenance initiative. The spares study has also led to pilot programs for sourcing alternatives that include regional intermediate inventories and partnering agreements that leverage existing supply chains. Many partners from the SLD Sustainability program contributed to and were consulted in the course of the study. This document provides a description of the findings, recommendations, and implemented solutions that have resulted from the study.

  8. Boron-Lined Neutron Detector Measurements

    SciTech Connect (OSTI)

    Lintereur, Azaree T.; Kouzes, Richard T.; Ely, James H.; Erikson, Luke E.; Siciliano, Edward R.; Woodring, Mitchell L.

    2010-03-07

    PNNL-18938 Revision Radiation portal monitors used for interdiction of illicit materials at borders include highly sensitive neutron detection systems. The main reason for having neutron detection capability is to detect fission neutrons from plutonium. The currently deployed radiation portal monitors (RPMs) from Ludlum and Science Applications International Corporation (SAIC) use neutron detectors based upon 3He-filled gas proportional counters, which are the most common large neutron detector. There is a declining supply of 3He in the world, and thus, methods to reduce the use of this gas in RPMs with minimal changes to the current system designs and sensitivity to cargo-borne neutrons are being investigated. Four technologies have been identified as being currently commercially available, potential alternative neutron detectors to replace the use of 3He in RPMs. Reported here are the results of tests of a newly designed boron-lined proportional counter option. This testing measured the neutron detection efficiency and gamma ray rejection capabilities of two successive prototypes of a system manufactured by GE Reuter Stokes.

  9. Boron-Lined Neutron Detector Measurements

    SciTech Connect (OSTI)

    Lintereur, Azaree T.; Kouzes, Richard T.; Ely, James H.; Erikson, Luke E.; Siciliano, Edward R.

    2009-11-02

    Radiation portal monitors used for interdiction of illicit materials at borders include highly sensitive neutron detection systems. The main reason for having neutron detection capability is to detect fission neutrons from plutonium. The currently deployed radiation portal monitors (RPMs) from Ludlum and Science Applications International Corporation (SAIC) use neutron detectors based upon 3He-filled gas proportional counters, which are the most common large neutron detector. There is a declining supply of 3He in the world, and thus, methods to reduce the use of this gas in RPMs with minimal changes to the current system designs and sensitivity to cargo-borne neutrons are being investigated. Four technologies have been identified as being currently commercially available, potential alternative neutron detectors to replace the use of 3He in RPMs. Reported here are the results of tests of a newly designed boron-lined proportional counter option. This testing measured the neutron detection efficiency and gamma ray rejection capabilities of a system manufactured by Reuter Stokes.

  10. Contingency Analysis of Cascading Line Outage Events

    SciTech Connect (OSTI)

    Thomas L Baldwin; Magdy S Tawfik; Miles McQueen

    2011-03-01

    As the US power systems continue to increase in size and complexity, including the growth of smart grids, larger blackouts due to cascading outages become more likely. Grid congestion is often associated with a cascading collapse leading to a major blackout. Such a collapse is characterized by a self-sustaining sequence of line outages followed by a topology breakup of the network. This paper addresses the implementation and testing of a process for N-k contingency analysis and sequential cascading outage simulation in order to identify potential cascading modes. A modeling approach described in this paper offers a unique capability to identify initiating events that may lead to cascading outages. It predicts the development of cascading events by identifying and visualizing potential cascading tiers. The proposed approach was implemented using a 328-bus simplified SERC power system network. The results of the study indicate that initiating events and possible cascading chains may be identified, ranked and visualized. This approach may be used to improve the reliability of a transmission grid and reduce its vulnerability to cascading outages.

  11. The transcriptional diversity of 25 Drosophila cell lines

    SciTech Connect (OSTI)

    Cherbas, Lucy; Willingham, Aarron; Zhang, Dayu; Yang, Li; Zou, Yi; Eads, Brian D.; Carlson, Joseph W.; Landolin, Jane M.; Kapranov, Philipp; Dumais, Jacqueline; Samsonova, Anastasia; Choi, Jeong-Hyeon; Roberts, Johnny; Davis, Carrie A.; Tang, Haixu; van Baren, Marijke J.; Ghosh, Srinka; Dobin, Alexander; Bell, Kim; Lin, Wei; Langton, Laura; Duff, Michael O.; Tenney, Aaron E.; Zaleski, Chris; Brent, Michael R.; Hoskins, Roger A.; Kaufman, Thomas C.; Andrews, Justen; Graveley, Brenton R.; Perrimon, Norbert; Celniker, Susan E.; Gingeras, Thomas R.; Cherbas, Peter

    2010-11-15

    Drosophila melanogaster cell lines are important resources for cell biologists. Here, we catalog the expression of exons, genes, and unannotated transcriptional signals for 25 lines. Unannotated transcription is substantial (typically 19% of euchromatic signal). Conservatively, we identify 1405 novel transcribed regions; 684 of these appear to be new exons of neighboring, often distant, genes. Sixty-four percent of genes are expressed detectably in at least one line, but only 21% are detected in all lines. Each cell line expresses, on average, 5885 genes, including a common set of 3109. Expression levels vary over several orders of magnitude. Major signaling pathways are well represented: most differentiation pathways are ‘‘off’’ and survival/growth pathways ‘‘on.’’ Roughly 50% of the genes expressed by each line are not part of the common set, and these show considerable individuality. Thirty-one percent are expressed at a higher level in at least one cell line than in any single developmental stage, suggesting that each line is enriched for genes characteristic of small sets of cells. Most remarkable is that imaginal discderived lines can generally be assigned, on the basis of expression, to small territories within developing discs. These mappings reveal unexpected stability of even fine-grained spatial determination. No two cell lines show identical transcription factor expression. We conclude that each line has retained features of an individual founder cell superimposed on a common ‘‘cell line‘‘ gene expression pattern. Wereport the transcriptional profiles of 25 Drosophila melanogaster cell lines, principally by whole-genome tiling microarray analysis of total RNA, carried out as part of the modENCODE project. The data produced in this study add to our knowledge of the cell lines and of the Drosophila transcriptome in several ways. We summarize the expression of previously annotated genes in each of the 25 lines with emphasis on what those patterns reveal about the origins of the lines and the stability of spatial expression patterns. We also offer an initial analysis of previously unannotated transcripts in the cell lines.

  12. Radio frequency communication system utilizing radiating transmission lines

    DOE Patents [OSTI]

    Struven, Warren C. (San Carlos, CA)

    1984-01-01

    A radio communication system for use in tunnels, mines, buildings or other shielded locations in which a pair of radiating transmission lines (30), (31) extend through such location in spaced coextensive relation to each other. Each transmission line (30), (31) has at least one unidirectional amplifier (32), (33) interposed therein with the sense of the unidirectional amplifier (32) of one transmission line (30) being opposite to the sense of the unidirectional amplifier (33) of the other transmission line (31). Each of the amplifiers (32), (33) has a gain which is less than the coupling loss between the transmission lines (30), (31). Two or more mobile transceivers (35) in the location served by the system are coupled to the transmission lines (30), (31) by electromagnetic wave propagation in space in order to communicate directly with each other at a given radio frequency within the frequency range of the system.

  13. Sensor, method and system of monitoring transmission lines

    DOE Patents [OSTI]

    Syracuse, Steven J.; Clark, Roy; Halverson, Peter G.; Tesche, Frederick M.; Barlow, Charles V.

    2012-10-02

    An apparatus, method, and system for measuring the magnetic field produced by phase conductors in multi-phase power lines. The magnetic field measurements are used to determine the current load on the conductors. The magnetic fields are sensed by coils placed sufficiently proximate the lines to measure the voltage induced in the coils by the field without touching the lines. The x and y components of the magnetic fields are used to calculate the conductor sag, and then the sag data, along with the field strength data, can be used to calculate the current load on the line and the phase of the current. The sag calculations of this invention are independent of line voltage and line current measurements. The system applies a computerized fitter routine to measured and sampled voltages on the coils to accurately determine the values of parameters associated with the overhead phase conductors.

  14. In-line phase shift tomosynthesis

    SciTech Connect (OSTI)

    Hammonds, Jeffrey C.; Price, Ronald R.; Pickens, David R.; Donnelly, Edwin F.

    2013-08-15

    Purpose: The purpose of this work is to (1) demonstrate laboratory measurements of phase shift images derived from in-line phase-contrast radiographs using the attenuation-partition based algorithm (APBA) of Yan et al.[Opt. Express 18(15), 16074–16089 (2010)], (2) verify that the APBA reconstructed images obey the linearity principle, and (3) reconstruct tomosynthesis phase shift images from a collection of angularly sampled planar phase shift images.Methods: An unmodified, commercially available cabinet x-ray system (Faxitron LX-60) was used in this experiment. This system contains a tungsten anode x-ray tube with a nominal focal spot size of 10 ?m. The digital detector uses CsI/CMOS with a pixel size of 50 × 50 ?m. The phantoms used consisted of one acrylic plate, two polystyrene plates, and a habanero pepper. Tomosynthesis images were reconstructed from 51 images acquired over a ±25° arc. All phase shift images were reconstructed using the APBA.Results: Image contrast derived from the planar phase shift image of an acrylic plate of uniform thickness exceeded the contrast of the traditional attenuation image by an approximate factor of two. Comparison of the planar phase shift images from a single, uniform thickness polystyrene plate with two polystyrene plates demonstrated an approximate linearity of the estimated phase shift with plate thickness (?1600 rad vs ?2970 rad). Tomographic phase shift images of the habanero pepper exhibited acceptable spatial resolution and contrast comparable to the corresponding attenuation image.Conclusions: This work demonstrated the feasibility of laboratory-based phase shift tomosynthesis and suggests that phase shift imaging could potentially provide a new imaging biomarker. Further investigation will be needed to determine if phase shift contrast will be able to provide new tissue contrast information or improved clinical performance.

  15. TRANSPORT OF WASTE SIMULANTS IN PJM VENT LINES

    SciTech Connect (OSTI)

    Qureshi, Z

    2007-02-21

    The experimental work was conducted to determine whether there is a potential for waste simulant to transport or 'creep' up the air link line and contaminate the pulse jet vent system, and possibly cause long term restriction of the air link line. Additionally, if simulant creep occurred, establish operating parameters for washing down the line. The amount of the addition of flush fluids and mixer downtime must be quantified.

  16. Monolithic torpedo bottle lining at Weirton Steel Corporation

    SciTech Connect (OSTI)

    Baker, R.; Griffith, E.

    1996-12-31

    In late 1992 and early 1993 Weirton Steel burned through three torpedo bottles in a three-month period. To determine the cause of the burn throughs, a thorough review of bottle maintenance practices was initiated. Upon identification of contributing factors, changes in operating practices were made. In an effort to increase bottle reliability, lining trials were initiated. Among the trials, a monolithic lining was installed and this paper will discuss results of the lining to date.

  17. Hydrogen transport diagnostics by atomic and molecular emission line

    Office of Scientific and Technical Information (OSTI)

    profiles simultaneously measured for large helical device (Journal Article) | SciTech Connect Hydrogen transport diagnostics by atomic and molecular emission line profiles simultaneously measured for large helical device Citation Details In-Document Search Title: Hydrogen transport diagnostics by atomic and molecular emission line profiles simultaneously measured for large helical device We observe the Balmer-{alpha}, -{beta}, and -{gamma} lines of hydrogen atoms and Q branches of the

  18. EIS-0463: Northern Pass Transmission Line Project, New Hampshire |

    Energy Savers [EERE]

    Department of Energy 463: Northern Pass Transmission Line Project, New Hampshire EIS-0463: Northern Pass Transmission Line Project, New Hampshire Summary This EIS will evaluate the potential environmental impacts from DOE's proposed Federal action of granting a Presidential permit to Northern Pass Transmission, LLC, to construct, operate, maintain, and connect a new electric transmission line across the U.S.-Canada border in northern New Hampshire. The U.S. Environmental Protection Agency

  19. Strengthening the Front Lines: Sales Training and Continuing Education for

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Contractors | Department of Energy the Front Lines: Sales Training and Continuing Education for Contractors Strengthening the Front Lines: Sales Training and Continuing Education for Contractors Better Buildings Residential Network Peer Exchange Call Series: Strengthening the Front Lines: Sales Training and Continuing Education for Contractors, call slides and discussion summary. PDF icon Call Slides and Discussion Summary More Documents & Publications Staged Upgrades - Homeowner-focused

  20. DOE Seeking Information on Transmission Line Permitting | Department of

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Energy Seeking Information on Transmission Line Permitting DOE Seeking Information on Transmission Line Permitting February 27, 2012 - 3:25pm Addthis The Department of Energy's Office of Electricity Delivery and Energy Reliability is seeking information on the questions related to permitting of transmission lines. Infrastructure projects - such as high voltage, long distance, electric transmission facilities - often involve multiple Federal, State, local, and Tribal authorizations and are

  1. Comments on RFI on Permitting of Transmission Lines Available | Department

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of Energy Comments on RFI on Permitting of Transmission Lines Available Comments on RFI on Permitting of Transmission Lines Available April 3, 2012 - 2:12pm Addthis On October 5, 2011, the White House announced the creation of a Rapid Response Team for Transmission (RRTT). In support of the RRTT the Department of Energy's Office of Electricity Delivery and Energy Reliability released a Federal Register Notice seeking information on questions related to permitting of transmission lines. The

  2. Inertial confinement fusion method producing line source radiation fluence

    DOE Patents [OSTI]

    Rose, Ronald P. (Peters Township, Washington County, PA)

    1984-01-01

    An inertial confinement fusion method in which target pellets are imploded in sequence by laser light beams or other energy beams at an implosion site which is variable between pellet implosions along a line. The effect of the variability in position of the implosion site along a line is to distribute the radiation fluence in surrounding reactor components as a line source of radiation would do, thereby permitting the utilization of cylindrical geometry in the design of the reactor and internal components.

  3. EIS-0507: Boardman-Hemingway Transmission Line, Oregon and Idaho |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy 7: Boardman-Hemingway Transmission Line, Oregon and Idaho EIS-0507: Boardman-Hemingway Transmission Line, Oregon and Idaho Summary The Bureau of Land Management and the U.S. Forest Service are preparing, with DOE's Bonneville Power Administration (BPA) as a cooperating agency, an EIS that evaluates the potential environmental impacts of a proposal to construct about 305 miles of 500-kV transmission line from northeast Oregon to southwest Idaho. BPA's proposed action is

  4. Section 1222 Program - Proposed Plains & Eastern Clean Line Project

    Energy Savers [EERE]

    Frequently Asked Questions | Department of Energy Section 1222 of the Energy Policy Act 2005 » Proposed Project: Plains & Eastern Clean Line » Section 1222 Program - Proposed Plains & Eastern Clean Line Project Frequently Asked Questions Section 1222 Program - Proposed Plains & Eastern Clean Line Project Frequently Asked Questions Note: DOE intends to update these FAQs as more information about the proposed project and the review process becomes available. FAQs 1. What is the

  5. Safety and Health Regulatory and Policy Response Line | Department of

    Energy Savers [EERE]

    Energy Safety and Health Regulatory and Policy Response Line Safety and Health Regulatory and Policy Response Line The Response Line is a service maintained by the Office of Worker Safety and Health Policy, AU-11, within the Office of Health and Safety, AU-10. It provides responses to questions from DOE and DOE contractor personnel regarding worker safety and health requirements and guidance. The information contained in responses are technical clarifications to a DOE Rule or Directive and

  6. Line Management Understanding of QA and Oversight | Department of Energy

    Energy Savers [EERE]

    Line Management Understanding of QA and Oversight Line Management Understanding of QA and Oversight Users will provide current or on-going QA issues of concern that impact work being done correctly, timely, and safely. Input could be from recent assessments, trends, Performance Metrics, number of open action items, recurring issues, etc. PDF icon Line Management Understanding of QA and Oversight More Documents & Publications SOPP-43, EM-23 Quality Assurance Oversight Protocol for EM

  7. Increasing the Capacity of Existing Power Lines | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Increasing the Capacity of Existing Power Lines Increasing the Capacity of Existing Power Lines The capacity of the grid has been largely unchanged for decades and needs to expand to accommodate new power plants and renewable energy projects. The difference in time and cost between using existing transmission lines or the construction of new ones can make or break plans for new wind or solar farms. PDF icon inl_powerline_cooling_factsheet.pdf More Documents & Publications EIS-0183: Record of

  8. EIS-0474: Southline Transmission Line Project; Arizona and New Mexico |

    Office of Environmental Management (EM)

    Department of Energy 74: Southline Transmission Line Project; Arizona and New Mexico EIS-0474: Southline Transmission Line Project; Arizona and New Mexico Summary The Bureau of Land Management and Western Area Power Administration are preparing an EIS as joint lead agencies to evaluate the potential environmental impacts of the proposed Southline Transmission Project and address associated potential land use plan amendments. The project would consist of a new 225-mile transmission line

  9. EIS-0499: Great Northern Transmission Line Project, Minnesota | Department

    Office of Environmental Management (EM)

    of Energy 9: Great Northern Transmission Line Project, Minnesota EIS-0499: Great Northern Transmission Line Project, Minnesota Summary This EIS will evaluate the potential environmental impacts of a DOE proposal to grant a Presidential permit to Minnesota Power to construct, operate, maintain, and connect a new 883-megawatt electric transmission system across the U.S.-Canada border. The proposed 220 mile transmission line would cross the border near Roseau, Minnesota, and continue to Grand

  10. EIS-0502: Hot Springs to Anaconda Transmission Line Rebuild Project,

    Office of Environmental Management (EM)

    Montana | Department of Energy 2: Hot Springs to Anaconda Transmission Line Rebuild Project, Montana EIS-0502: Hot Springs to Anaconda Transmission Line Rebuild Project, Montana Summary DOE's Bonneville Power Administration (BPA) is preparing an EIS that will analyze the potential environmental impacts of a proposal to rebuild approximately 120 miles of existing transmission line in Sanders, Lake, Missoula, Granite, Powell, and Deer Lodge Counties in Montana. EIS-0502: Hot Springs to

  11. Appendix S-51 - Transmission Line Approval - Public Utilities...

    Open Energy Info (EERE)

    from "http:en.openei.orgwindex.php?titleAppendixS-51-TransmissionLineApproval-PublicUtilitiesCommission&oldid800735" Feedback Contact needs updating Image needs...

  12. Office of Enterprise Assessments Review of Field Element Line...

    Office of Environmental Management (EM)

    SNL Sandia National Laboratories ii Office of Enterprise Assessments Review of Field Element Line Management Oversight at Sandia National Laboratories EXECUTIVE SUMMARY The...

  13. EIS-0499: Great Northern Transmission Line Project, Minnesota...

    Broader source: Energy.gov (indexed) [DOE]

    and connect a new 883-megawatt electric transmission system across the U.S.-Canada border. The proposed 220 mile transmission line would cross the border near Roseau,...

  14. Magnetic Field Line Tracing Calculations for Conceptual PFC Design...

    Office of Scientific and Technical Information (OSTI)

    Conference: Magnetic Field Line Tracing Calculations for Conceptual PFC Design in the National Compact Stellarator Experiment Citation Details In-Document Search Title: Magnetic...

  15. Section 1222 Program - Proposed Plains & Eastern Clean Line Project...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Section 1222 Program - Proposed Plains & Eastern Clean Line ... through the Southwestern Power Administration ... NEPA Website, and through other channels of communication. ...

  16. A simple line wave generator using commercial explosives

    SciTech Connect (OSTI)

    Morris, John S; Jackson, Scott I; Hill, Larry G

    2009-01-01

    We present a simple and inexpensive explosive line wave generator has been designed using commercial sheet explosive and plane wave lens concepts. The line wave generator is constructed using PETN and RDX based sheet explosive for the slow and fast components respectively. The design permits the creation of any desired line width. A series of experiments were performed on a 100 mm design, measuring the detonation arrival time at the output of the generator using a streak camera. An iterative technique was used to adjust the line wave generator's slow and fast components, so as to minimize the arrival time deviation. Designs, test results, and concepts for improvements will be discussed.

  17. Development of aerogel-lined targets for inertial confinement...

    Office of Scientific and Technical Information (OSTI)

    for inertial confinement fusion experiments Citation Details In-Document Search Title: Development of aerogel-lined targets for inertial confinement fusion experiments Authors: ...

  18. Magnetic Field Line Tracing Calculations for Conceptual PFC Design...

    Office of Scientific and Technical Information (OSTI)

    PFC Design in the National Compact Stellarator Experiment Citation Details In-Document Search Title: Magnetic Field Line Tracing Calculations for Conceptual PFC Design in the ...

  19. Electrical and Biological Effects of Transmission Lines: A Review.

    SciTech Connect (OSTI)

    Lee, Jack M.

    1989-06-01

    This review describes the electrical properties of a-c and d-c transmission lines and the resulting effects on plants, animals, and people. Methods used by BPA to mitigate undesirable effects are also discussed. Although much of the information in this review pertains to high-voltage transmission lines, information on distribution lines and electrical appliances is included. The electrical properties discussed are electric and magnetic fields and corona: first for alternating-current (a-c) lines, then for direct current (d-c).

  20. Energy Department Announces Participation in Clean Line's Large...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... improvements; In response to public input, the Clean Line project will include a 500 megawatt converter station in Arkansas to ensure that consumers in the state can benefit ...

  1. Notice of Availability of Plains & Eastern Clean Line Transmission...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Availability of Plains & Eastern Clean Line Transmission Project Draft Environmental Impact Statement and Public Hearings: Federal Register Notice, Volume 79, No. 242 - Dec. 17, ...

  2. EIS-0474: Southline Transmission Line Project; Arizona and New...

    Broader source: Energy.gov (indexed) [DOE]

    consist of a new 225-mile transmission line between existing substations at Afton, New Mexico, and Apache, Arizona, and improvements to approximately 130 miles of existing...

  3. Proposed Southline Transmission Line Project - Volume 4 of 4...

    Office of Environmental Management (EM)

    2 Appendix J J-1 Southline Transmission Line Project Draft Environmental Impact Statement and Draft Resource Management Plan Amendment BLM LAND USE AUTHORIZATIONS 1...

  4. EIS-0317: Kangley-Echo Lake Transmission Line Project

    Broader source: Energy.gov [DOE]

    This EIS analyzes BPA's proposal to build a new transmission line to accommodate increasing demand for electricity and ensure reliability in the Puget Sound area.

  5. Strengthening the Front Lines: Sales Training and Continuing...

    Broader source: Energy.gov (indexed) [DOE]

    Strengthening the Front Lines: Sales Training and Continuing Education for Contractors, call slides and discussion summary. Call Slides and Discussion Summary More Documents &...

  6. EIS-0325: Schultz-Hanford Area Transmission Line Project, WA

    Broader source: Energy.gov [DOE]

    BPA proposes to construct a new 500-kilovolt (kV) transmission line in central Washington. This project would increase transmission system capacity north of Hanford.

  7. Historical Time Line and Information About the Hanford Site

    SciTech Connect (OSTI)

    Briggs, James D.

    2001-03-07

    Historical time line of the Hanford Site spanning from 1940 through 1997, including photographs and other information regarding the town sites and living conditions.

  8. Hawaii Clean Energy Initiative - Transmission Line Approval Permit...

    Open Energy Info (EERE)

    Reference LibraryAdd to library PermittingRegulatory Guidance - Instructions: Hawaii Clean Energy Initiative - Transmission Line Approval Permit PacketPermittingRegulatory...

  9. EIS-0502: Hot Springs to Anaconda Transmission Line Rebuild Project...

    Energy Savers [EERE]

    Springs to Anaconda Transmission Line Rebuild Project, Montana SUMMARY DOE's Bonneville Power Administration (BPA) is preparing an EIS that will analyze the potential environmental...

  10. TechLine: Newly Released Study Highlights Significant Utica Shale...

    Office of Environmental Management (EM)

    TechLine: Newly Released Study Highlights Significant Utica Shale Potential Results from NETL-Sponsored Study Now Publically Available A pioneering study led by West Virginia ...

  11. Suggested Practices for Avian Protection on Power Lines | Open...

    Open Energy Info (EERE)

    with federal laws protecting and enhancing avian populations, and maintaining the reliability of electric power networds. Author Avian Power Line Interaction Committee...

  12. Development of aerogel-lined targets for inertial confinement...

    Office of Scientific and Technical Information (OSTI)

    aerogel-lined targets for inertial confinement fusion experiments Braun, T 36 MATERIALS SCIENCE Abstract not provided Lawrence Livermore National Laboratory (LLNL), Livermore, CA...

  13. Guide to Permitting Electric Transmission Lines in Wyoming |...

    Open Energy Info (EERE)

    search OpenEI Reference LibraryAdd to library PermittingRegulatory Guidance - GuideHandbook: Guide to Permitting Electric Transmission Lines in WyomingPermitting...

  14. Sandia Energy - Digital In-Line Holography Helps Researchers...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    droplets of fuel are generated and behave in such cases. Sandia researchers developed 3-D measurement techniques based on digital in-line holography (DIH). Sandia advanced DIH...

  15. Analytical models of calcium binding in a calcium channel

    SciTech Connect (OSTI)

    Liu, Jinn-Liang; Eisenberg, Bob

    2014-08-21

    The anomalous mole fraction effect of L-type calcium channels is analyzed using a Fermi like distribution with the experimental data of Almers and McCleskey [J. Physiol. 353, 585 (1984)] and the atomic resolution model of Lipkind and Fozzard [Biochemistry 40, 6786 (2001)] of the selectivity filter of the channel. Much of the analysis is algebraic, independent of differential equations. The Fermi distribution is derived from the configuration entropy of ions and water molecules with different sizes, different valences, and interstitial voids between particles. It allows us to calculate potentials and distances (between the binding ion and the oxygen ions of the glutamate side chains) directly from the experimental data using algebraic formulas. The spatial resolution of these results is comparable with those of molecular models, but of course the accuracy is no better than that implied by the experimental data. The glutamate side chains in our model are flexible enough to accommodate different types of binding ions in different bath conditions. The binding curves of Na{sup +} and Ca{sup 2+} for [CaCl{sub 2}] ranging from 10{sup −8} to 10{sup −2} M with a fixed 32 mM background [NaCl] are shown to agree with published Monte Carlo simulations. The Poisson-Fermi differential equation—that includes both steric and correlation effects—is then used to obtain the spatial profiles of energy, concentration, and dielectric coefficient from the solvent region to the filter. The energy profiles of ions are shown to depend sensitively on the steric energy that is not taken into account in the classical rate theory. We improve the rate theory by introducing a steric energy that lumps the effects of excluded volumes of all ions and water molecules and empty spaces between particles created by Lennard-Jones type and electrostatic forces. We show that the energy landscape varies significantly with bath concentrations. The energy landscape is not constant.

  16. Hydroxyapatite-binding peptides for bone growth and inhibition

    DOE Patents [OSTI]

    Bertozzi, Carolyn R. (Berkeley, CA); Song, Jie (Shrewsbury, MA); Lee, Seung-Wuk (Walnut Creek, CA)

    2011-09-20

    Hydroxyapatite (HA)-binding peptides are selected using combinatorial phage library display. Pseudo-repetitive consensus amino acid sequences possessing periodic hydroxyl side chains in every two or three amino acid sequences are obtained. These sequences resemble the (Gly-Pro-Hyp).sub.x repeat of human type I collagen, a major component of extracellular matrices of natural bone. A consistent presence of basic amino acid residues is also observed. The peptides are synthesized by the solid-phase synthetic method and then used for template-driven HA-mineralization. Microscopy reveal that the peptides template the growth of polycrystalline HA crystals .about.40 nm in size.

  17. Functionalized polymers for binding to solutes in aqueous solutions

    DOE Patents [OSTI]

    Smith, Barbara F.; Robison, Thomas W.

    2006-11-21

    A functionalized polymer for binding a dissolved molecule in an aqueous solution is presented. The polymer has a backbone polymer to which one or more functional groups are covalently linked. The backbone polymer can be such polymers as polyethylenimine, polyvinylamine, polyallylamine, and polypropylamine. These polymers are generally water-soluble, but can be insoluble when cross-linked. The functional group can be for example diol derivatives, polyol derivatives, thiol and dithiol derivatives, guest-host groups, affinity groups, beta-diphosphonic acids, and beta-diamides

  18. Breaking the ties that bind: New hope for biomass fuels

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    New hope for biomass fuels Breaking the ties that bind: New hope for biomass fuels Researchers have discovered a potential chink in the armor of fibers that make the cell walls of certain inedible plant materials so tough. April 22, 2009 Los Alamos National Laboratory sits on top of a once-remote mesa in northern New Mexico with the Jemez mountains as a backdrop to research and innovation covering multi-disciplines from bioscience, sustainable energy sources, to plasma physics and new materials.

  19. Highly-Selective and Reversible O2 Binding in

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Cr3(1,3,5-benzenetricarboxylate)2 | Center for Gas SeparationsRelevant to Clean Energy Technologies | Blandine Jerome Highly-Selective and Reversible O2 Binding in Cr3(1,3,5-benzenetricarboxylate)2 Previous Next List Leslie J. Murray, Mircea Dinca, Junko Yano, Sachin Chavan, Silvia Bordiga, Craig M. Brown and Jeffrey R. Long, J. Am. Chem. Soc., 2010, 132 (23), pp 7856-7857 DOI: 10.1021/ja1027925 Abstract Image Abstract: Reaction of Cr(CO)6 with trimesic acid in DMF affords the metal-organic

  20. The impact of gas bulk rotation on the Ly? line

    SciTech Connect (OSTI)

    Garavito-Camargo, Juan N.; Forero-Romero, Jaime E.; Dijkstra, Mark E-mail: je.forero@uniandes.edu.co

    2014-11-10

    We present results of radiative transfer calculations to measure the impact of gas bulk rotation on the morphology of the Ly? emission line in distant galaxies. We model a galaxy as a sphere with an homogeneous mixture of dust and hydrogen at a constant temperature. These spheres undergo solid-body rotation with maximum velocities in the range 0-300 km s{sup –1} and neutral hydrogen optical depths in the range ?{sub H} = 10{sup 5}-10{sup 7}. We consider two types of source distributions in the sphere: central and homogeneous. Our main result is that rotation introduces a dependence of the line morphology with viewing angle and rotational velocity. Observations with a line of sight parallel to the rotation axis yield line morphologies similar to the static case. For lines of sight perpendicular to the rotation axis, both the intensity at the line center and the line width increase with rotational velocity. Along the same line of sight, the line becomes single peaked at rotational velocities close to half the line width in the static case. Notably, we find that rotation does not induce any spatial anisotropy in the integrated line flux, the escape fraction or the average number of scatterings. This is because Lyman scattering through a rotating solid-body proceeds identically to the static case. The only difference is the Doppler shift from the different regions in the sphere that move with respect to the observer. This allows us to derive an analytic approximation for the viewing-angle dependence of the emerging spectrum, as a function of rotational velocity.

  1. Situ soil sampling probe system with heated transfer line

    DOE Patents [OSTI]

    Robbat, Jr., Albert (Andover, MA)

    2002-01-01

    The present invention is directed both to an improved in situ penetrometer probe and to a heated, flexible transfer line. The line and probe may be implemented together in a penetrometer system in which the transfer line is used to connect the probe to a collector/analyzer at the surface. The probe comprises a heater that controls a temperature of a geologic medium surrounding the probe. At least one carrier gas port and vapor collection port are located on an external side wall of the probe. The carrier gas port provides a carrier gas into the geologic medium, and the collection port captures vapors from the geologic medium for analysis. In the transfer line, a flexible collection line that conveys a collected fluid, i.e., vapor, sample to a collector/analyzer. A flexible carrier gas line conveys a carrier gas to facilitate the collection of the sample. A system heating the collection line is also provided. Preferably the collection line is electrically conductive so that an electrical power source can generate a current through it so that the internal resistance generates heat.

  2. Line Management Perspective: Office of Environmental Management (EM) |

    Energy Savers [EERE]

    Department of Energy Line Management Perspective: Office of Environmental Management (EM) Line Management Perspective: Office of Environmental Management (EM) Addthis Description Slide Presentation by Matthew Moury, Deputy Assistant Secretary for Safety, Security and Quality Programs, Office of Environmental Management. EFCOG Integrated Safety Management Work Planning and Control. Biography: Matthew Moury View All Videos

  3. Continuous human cell lines and method of making same

    DOE Patents [OSTI]

    Stampfer, Martha R. (Oakland, CA)

    1989-01-01

    Substantially genetically stable continuous human cell lines derived from normal human mammary epithelial cells (HMEC) and processes for making and using the same. In a preferred embodiment, the cell lines are derived by treating normal human mammary epithelial tissue with a chemical carcinogen such as benzo[a]pyrene. The novel cell lines serve as useful substrates for elucidating the potential effects of a number of toxins, carcinogens and mutagens as well as of the addition of exogenous genetic material. The autogenic parent cells from which the cell lines are derived serve as convenient control samples for testing. The cell lines are not neoplastically transformed, although they have acquired several properties which distinguish them from their normal progenitors.

  4. Continuous human cell lines and method of making same

    DOE Patents [OSTI]

    Stampfer, M.R.

    1985-07-01

    Substantially genetically stable continuous human cell lines derived from normal human mammary epithelial cells (HMEC) and processes for making and using the same. In a preferred embodiment, the cell lines are derived by treating normal human mammary epithelial tissue with a chemical carcinogen such as benzo(a)pyrene. The novel cell lines serve as useful substrates for elucidating the potential effects of a number of toxins, carcinogens and mutagens as well as of the addition of exogenous genetic material. The autogenic parent cells from which the cell lines are derived serve as convenient control samples for testing. The cell lines are not neoplastically transformed, although they have acquired several properties which distinguish them from their normal progenitors. 2 tabs.

  5. Yield Line Evaluation Methodology for Reinforced Concrete Structures

    Energy Science and Technology Software Center (OSTI)

    1998-12-30

    Yield line theory is an analytical technique that can be used to determine the ultimate bending capacity of flat reinforced concrete plates subject to distributed and concentrated loadings. Alternately, yield line theory, combined with rotation limits can be used to determine the energy absorption capacity of plates subject to impulsive and impact loadings. Typical components analyzed by yield line theory are basemats, floor and roof slabs subject to vertical loads along with walls subject tomore » out of plane loadings. One limitation of yield line theory is that it is computationally difficult to evaluate some mechanisms. This problem is aggravated by the complex geometry and reinforcing layouts commonly found in practice. The program has the capability to either evaluate a single user defined mechanism or to iterate over a range of mechanisms to determine the minimum ultimate capacity. The program is verified by comparison to a series of yield line mechanisms with known solutions.« less

  6. Pathogenicity of the BRCA1 Missense Variant M1775K is Determined by the Disruption of the BRCT Phosphopeptide-Binding Pocket: a Multi-Modal Approach

    SciTech Connect (OSTI)

    Tischkowitz,M.; Hamel, N.; Carvalho, M.; Birrane, G.; Soni, A.; van Beers, E.; Joosse, S.; Wong, N.; Novak, D.; et al

    2008-01-01

    A number of germ-line mutations in the BRCA1 gene confer susceptibility to breast and ovarian cancer. However, it remains difficult to determine whether many single amino-acid (missense) changes in the BRCA1 protein that are frequently detected in the clinical setting are pathologic or not. Here, we used a combination of functional, crystallographic, biophysical, molecular and evolutionary techniques, and classical genetic segregation analysis to demonstrate that the BRCA1 missense variant M1775K is pathogenic. Functional assays in yeast and mammalian cells showed that the BRCA1 BRCT domains carrying the amino-acid change M1775K displayed markedly reduced transcriptional activity, indicating that this variant represents a deleterious mutation. Importantly, the M1775K mutation disrupted the phosphopeptide-binding pocket of the BRCA1 BRCT domains, thereby inhibiting the BRCA1 interaction with the proteins BRIP1 and CtIP, which are involved in DNA damage-induced checkpoint control. These results indicate that the integrity of the BRCT phosphopeptide-binding pocket is critical for the tumor suppression function of BRCA1. Moreover, this study demonstrates that multiple lines of evidence obtained from a combination of functional, structural, molecular and evolutionary techniques, and classical genetic segregation analysis are required to confirm the pathogenicity of rare variants of disease-susceptibility genes and obtain important insights into the underlying pathogenetic mechanisms.

  7. Profiled spectral lines generated in the field of Kerr superspinars

    SciTech Connect (OSTI)

    Schee, Jan; Stuchlík, Zdenek E-mail: zdenek.stuchlik@fpf.slu.cz

    2013-04-01

    String Theory suggests existence of primordial Kerr superspinars, extremely compact objects with external spacetime described by the Kerr naked singularity geometry. The primordial Kerr superspinars have to be converted to a near-extreme black hole due to accretion, but they could survive to the era of highly redshifted quasars. We study the shape of the profiled spectral lines generated by radiating rings or the innermost parts of Keplerian discs orbiting the Kerr superspinars. Influence of the superspinar surface location on the profiled lines is also considered. We demonstrate strong difference of the character of the profiled lines generated by radiating rings for all values of the superspinar spin and all values of the inclination angles of the observer when compared to those generated in the field of Kerr black holes. For small and mediate inclination angles there are large quantitative differences in the extension and position of the lines. For large inclination angles even strong qualitative difference appears as the profiled lines have a clear doubled character. The smaller, redshifted region of the profiled line is related to the photons reaching the regions near the superspinar surface. Strong differences are obtained also for profiled lines generated by the innermost parts of Keplerian discs especially in the shape of the line. The influence of the superspinar surface location is reflected in the intermediate parts of the the profiled lines. The line profiles can give a clear signature of the presence of a Kerr superspinar and in principle enable estimates of its surface location since the signatures of the superspinar surface location are of different character as those corresponding to the presence of the black hole horizon.

  8. Methods and apparatus for reducing corrosion in refractory linings

    DOE Patents [OSTI]

    Poeppel, Roger B. (Glen Ellyn, IL); Greenberg, Sherman (Oak Lawn, IL); Diercks, Dwight R. (Plainfield, IL)

    1987-01-01

    Methods and apparatus are provided for reducing corrosion in a refractory lining of a liquid-containing vessel used in direct steelmaking processes. The vessel operates at between about 1600.degree. C. and about 1800.degree. C. and an oxygen partial pressure of about 10.sup.-12 atmospheres, creating slag which is rich in FeO. The refractory lining includes a significant level of chromium oxide (Cr.sub.2 O.sub.3), and has small interconnected pores which may be filled with a gas mixture having a higher total pressure and oxygen partial pressure than the total pressure and oxygen partial pressure associted with the liquid against the lining of the vessel. The gas mixture is forced through the pores of the lining so that the pores are continuously filled with the mixture. In this manner, the gas mixture creates a blanket which increases the oxygen partial pressure at the lining enough to maintain the chromium in the lining in a selected valence state in which the chromium has decreased solubility in the FeO slag, thereby reducing corrosion by the FeO and increasing the useful life of the refractory lining.

  9. Laboratory Measurements of White Dwarf Photospheric Spectral Lines. Hβ

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Falcon, Ross Edward; Sandia National Lab.; Rochau, Gregory A.; Bailey, James E.; Gomez, Thomas; Sandia National Lab.; Montgomery, Michael Houston; Winget, Donald E; Nagayama, Taisuke

    2015-06-18

    We spectroscopically measure multiple hydrogen Balmer line profiles from laboratory plasmas to investigate the theoretical line profiles used in white dwarf (WD) atmosphere models. X-ray radiation produced at the Z Pulsed Power Facility at Sandia National Laboratories initiates plasma formation in a hydrogen-filled gas cell, replicating WD photospheric conditions. We also present time-resolved measurements of Hβ and fit this line using different theoretical line profiles to diagnose electron density, ne, and n = 2 level population, n2. Aided by synthetic tests, we characterize the validity of our diagnostic method for this experimental platform. During a single experiment, we infer amore » continuous range of electron densities increasing from ne ~ 4 to ~30 × 1016 cm-3 throughout a 120-ns evolution of our plasma. Also, we observe n2 to be initially elevated with respect to local thermodynamic equilibrium (LTE); it then equilibrates within ~55 ns to become consistent with LTE. This also supports our electron-temperature determination of Te ~ 1.3 eV (~15,000 K) after this time. At ne≲ 1017 cm-3, we find that computer-simulation-based line-profile calculations provide better fits (lower reduced χ2) than the line profiles currently used in the WD astronomy community. The inferred conditions, however, are in good quantitative agreement. This work establishes an experimental foundation for the future investigation of relative shapes and strengths between different hydrogen Balmer lines.« less

  10. Applying a Modified Triad Approach to Investigate Wastewater lines

    SciTech Connect (OSTI)

    Pawlowicz, R.; Urizar, L.; Blanchard, S.; Jacobsen, K.; Scholfield, J.

    2006-07-01

    Approximately 20 miles of wastewater lines are below grade at an active military Base. This piping network feeds or fed domestic or industrial wastewater treatment plants on the Base. Past wastewater line investigations indicated potential contaminant releases to soil and groundwater. Further environmental assessment was recommended to characterize the lines because of possible releases. A Remedial Investigation (RI) using random sampling or use of sampling points spaced at predetermined distances along the entire length of the wastewater lines, however, would be inefficient and cost prohibitive. To accomplish RI goals efficiently and within budget, a modified Triad approach was used to design a defensible sampling and analysis plan and perform the investigation. The RI task was successfully executed and resulted in a reduced fieldwork schedule, and sampling and analytical costs. Results indicated that no major releases occurred at the biased sampling points. It was reasonably extrapolated that since releases did not occur at the most likely locations, then the entire length of a particular wastewater line segment was unlikely to have contaminated soil or groundwater and was recommended for no further action. A determination of no further action was recommended for the majority of the waste lines after completing the investigation. The modified Triad approach was successful and a similar approach could be applied to investigate wastewater lines on other United States Department of Defense or Department of Energy facilities. (authors)

  11. Development of Gamma-Emitting Receptor Binding Radiopharmace

    SciTech Connect (OSTI)

    Reba, Richard

    2003-02-20

    The long-term objective is to develop blood-brain barrier (BBB) permeable m2-selective (relative to m1, m3, and m4) receptor-binding radiotracers and utilize these radiotracers for quantifying receptor concentrations obtained from PET or SPECT images of human brain. In initial studies, we concluded that the lipophilicity and high affinity prevented (R,S)-I-QNB from reaching a flow-independent and receptor-dependent state in a reasonable time. Thus, it was clear that (R,S)-I-QNB should be modified. Therefore, during the last portion of this funded research, we proposed that more polar heterocycles should help accomplish that. Since reports of others concluded that radiobromination and radiofluorination of the unactivated phenyl ring is not feasible (Newkome et al,,1982), we, therefore, explored during this grant period a series of analogues of (R)-QNB in which one or both of the six-membered phenyl rings is replaced by a five-membered thienyl (Boulay et al., 1995), or furyl ring. The chemistry specific aims were to synthesize novel compounds designed to be m2-selective mAChR ligands capable of penetrating into the CNS, and develop methods for efficient radiolabeling of promising m2-selective muscarinic ligands. The pharmacology specific aims were to determine the affinity and subtype-selectivity of the novel compounds using competition binding studies with membranes from cells that express each of the five muscarinic receptor subtypes, to determine the ability of the promising non-radioactive compounds and radiolabeled novel compounds to cross the BBB, to determine the biodistribution, in-vivo pharmacokinetics, and in-vitm kinetics of promising m2-selective radioligands and to determine the distribution of receptors for the novel m2-selective radioligands using quantitative autoradiography of rat brain, and compare this distribution to the distribution of known m2-selective compounds.

  12. Phase II beam lines at the National Synchrotron Light Source

    SciTech Connect (OSTI)

    Thomlinson, W.

    1984-06-01

    The expansion of the National Synchrotron Light Source has been funded by the US Department of Energy. The Phase II program consists of both increased conventional facilities and six new beam lines. In this paper, an overview of the six beam lines which will be constructed during Phase II is presented. For five of the lines special radiation sources are necessary and the designs of four of the devices are complete. The relevant parameters of the insertion devices under construction and development are presented.

  13. Microsoft Word - WIPP Water Line _2_ _2_ _2_

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Announces the Transfer of the WIPP Water Line to the City of Carlsbad CARLSBAD, N.M., June 18, 2009-The U.S. Department of Energy announced today that the Carlsbad Field Office will transfer the DOE-owned Waste Isolation Pilot Plant (WIPP) water line to the city of Carlsbad. The move will provide the city with an additional water supply while saving the Department of Energy thousands of dollars. DOE first installed the water line in 1984 to support the ongoing operations of the Department's

  14. EM, County Install Sewer Line for Development | Department of Energy

    Office of Environmental Management (EM)

    EM, County Install Sewer Line for Development EM, County Install Sewer Line for Development April 29, 2014 - 3:53pm Addthis The Pike County Commissioners recently toured the construction site of the county’s new sewer line extension being installed. Shown with team members from contractors FOILL Construction, Stantec, and Fluor-B&W Portsmouth in the background are, front row, left to right, Dennis Carr of Fluor-B&W Portsmouth; Commissioners Teddy West, Blaine Beekman, and Harry

  15. New perspective on glycoside hydrolase binding to lignin from pretreated corn stover

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Yarbrough, John M.; Mittal, Ashutosh; Mansfield, Elisabeth; Taylor, II, Larry E.; Hobdey, Sarah E.; Sammond, Deanne W.; Bomble, Yannick J.; Crowley, Michael F.; Decker, Stephen R.; Himmel, Michael E.; et al

    2015-12-18

    In this study, non-specific binding of cellulases to lignin has been implicated as a major factor in the loss of cellulase activity during biomass conversion to sugars. It is believed that this binding may strongly impact process economics through loss of enzyme activities during hydrolysis and enzyme recycling scenarios. The current model suggests glycoside hydrolase activities are lost though non-specific/non-productive binding of carbohydrate-binding domains to lignin, limiting catalytic site access to the carbohydrate components of the cell wall.

  16. U-227: bind-dyndb-ldap DN Escaping Flaw Lets Remote Users Deny Service

    Broader source: Energy.gov [DOE]

    A vulnerability has been reported in bind-dyndb-ldap, which can be exploited by malicious people to cause a DoS (Denial of Service).

  17. V-172: ISC BIND RUNTIME_CHECK Error Lets Remote Users Deny Service Against Recursive Resolvers

    Broader source: Energy.gov [DOE]

    A defect exists which allows an attacker to crash a BIND 9 recursive resolver with a RUNTIME_CHECK error in resolver.c

  18. CRAD, NNSA- Evaluation Site Office Line Oversight Programs

    Broader source: Energy.gov [DOE]

    CRAD for Evaluation Site Office Line Oversight Programs. Criteria Review and Approach Documents (CRADs) that can be used to conduct a well-organized and thorough assessment of elements of safety and health programs.

  19. Federal Line Management Oversight of Department of Energy Nuclear Facilities

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2013-04-04

    The Guide was developed in support of DOE O 226.1B to provide guidance that may be useful to DOE line management organizations in meeting the provisions of that order when applied to nuclear facilities.

  20. Single transmission line interrogated multiple channel data acquisition system

    DOE Patents [OSTI]

    Fasching, George E. (Morgantown, WV); Keech, Jr., Thomas W. (Morgantown, WV)

    1980-01-01

    A single transmission line interrogated multiple channel data acquisition system is provided in which a plurality of remote station/sensor circuits each monitors a specific process variable and each transmits measurement values over a single transmission line to a master interrogating station when addressed by said master interrogating station. Typically, as many as 330 remote stations may be parallel connected to the transmission line which may exceed 7,000 feet. The interrogation rate is typically 330 stations/second. The master interrogating station samples each station according to a shared, charging transmit-receive cycle. All remote station address signals, all data signals from the remote stations/sensors and all power for all of the remote station/sensors are transmitted via a single continuous terminated coaxial cable. A means is provided for periodically and remotely calibrating all remote sensors for zero and span. A provision is available to remotely disconnect any selected sensor station from the main transmission line.

  1. Federal Line Management Oversight of Department of Energy Nuclear...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    CURRENT DOE G 226.1-2A, Federal Line Management Oversight of Department of Energy Nuclear Facilities by David Weitzman Functional areas: Safety, Oversight The Guide was developed...

  2. Request for Proposals for New or Upgraded Transmission Line Projects...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Proposals for New or Upgraded Transmission Line Projects Under Section 1222 of the Energy Policy Act of 2005: Federal Register Notice Volume 75, No. 111 - Jun 10, 2012 Request for ...

  3. Grand Valley Rrl Pwr Line, Inc | Open Energy Information

    Open Energy Info (EERE)

    Valley Rrl Pwr Line, Inc Place: Colorado Website: www.gvp.org Twitter: @GVRuralPower Outage Hotline: 970-242-0040 Outage Map: www.gvp.orgcontentoutage-map References: EIA Form...

  4. EA-1891: Alvey-Fairview Transmission Line Rebuild Project, Oregon

    Broader source: Energy.gov [DOE]

    This EA evaluates the environmental impacts of a proposal to rebuild DOE’s Bonneville Power Administration’s Alvey-Fairview No. 1 230-kV transmission line located between Eugene, Oregon, and Coquille, Oregon.

  5. Internal-integral sodium return line for sodium heat engine

    DOE Patents [OSTI]

    Hunt, Thomas K. (Ann Arbor, MI)

    1985-01-01

    A thermoelectric generator device which converts heat energy to electrical energy. An alkali metal is used with a solid electrolyte and a portion of the return line for the alkali metal is located within the generator vacuum space.

  6. DOE Sustainability Reporting Open Line Help Call | Department...

    Broader source: Energy.gov (indexed) [DOE]

    November 18, 2015 2:00PM to 3:00PM EST The Sustainability Performance Office (SPO) sponsors open line help calls to assist DOE sites and national laboratories with the annual...

  7. DOE Sustainability Reporting Open Line Help Call | Department...

    Broader source: Energy.gov (indexed) [DOE]

    November 4, 2015 2:00PM to 3:00PM EST The Sustainability Performance Office (SPO) sponsors open line help calls to assist DOE sites and national laboratories with the annual...

  8. DOE Sustainability Reporting Open Line Help Call | Department...

    Broader source: Energy.gov (indexed) [DOE]

    October 28, 2015 2:00PM to 3:00PM EDT The Sustainability Performance Office (SPO) sponsors open line help calls to assist DOE sites and national laboratories with the annual...

  9. Guide to good practices for line and training manager activities

    SciTech Connect (OSTI)

    1998-06-01

    The purpose of this guide is to provide direction for line and training managers in carrying out their responsibilities for training and qualifying personnel and to verify that existing training activities are effective.

  10. DOE Sustainability Reporting Open Line Help Call | Department...

    Broader source: Energy.gov (indexed) [DOE]

    the SPO will be available on the call to answer specific questions with respect to Site Sustainability Plans (SSP) and the Consolidated Energy Data Report (CEDR). The Open Line...

  11. Soliton communication lines based on spectrally efficient modulation formats

    SciTech Connect (OSTI)

    Yushko, O V; Redyuk, A A

    2014-06-30

    We report the results of mathematical modelling of optical-signal propagation in soliton fibre-optic communication lines (FOCLs) based on spectrally efficient signal modulation formats. We have studied the influence of spontaneous emission noise, nonlinear distortions and FOCL length on the data transmission quality. We have compared the characteristics of a received optical signal for soliton and conventional dispersion compensating FOCLs. It is shown that in the presence of strong nonlinearity long-haul soliton FOCLs provide a higher data transmission performance, as well as allow higher order modulation formats to be used as compared to conventional communication lines. In the context of a coherent data transmission, soliton FOCLs allow the use of phase modulation with many levels, thereby increasing the spectral efficiency of the communication line. (optical communication lines)

  12. Libby (FEC)-Troy 115kV Line Rebuild

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of McNary Group 2 - Big Eddy to Knight The Big Eddy - Knight 500 kV line and new substation (WOMR Group 2) project is one of five projects recommended out of the 2008 Cluster...

  13. EIS-0106: Great Falls-Conrad Transmission Line Project, Montana

    Broader source: Energy.gov [DOE]

    The Western Area Power Administration prepared this EIS to evaluate the environmental impacts of the construction and operation of a 230-kilovolt transmission line from Great Falls, Montana, to Conrad, Montana.

  14. Wireless Sensor Network for Electric Transmission Line Monitoring

    SciTech Connect (OSTI)

    Alphenaar, Bruce

    2009-06-30

    Generally, federal agencies tasked to oversee power grid reliability are dependent on data from grid infrastructure owners and operators in order to obtain a basic level of situational awareness. Since there are many owners and operators involved in the day-to-day functioning of the power grid, the task of accessing, aggregating and analyzing grid information from these sources is not a trivial one. Seemingly basic tasks such as synchronizing data timestamps between many different data providers and sources can be difficult as evidenced during the post-event analysis of the August 2003 blackout. In this project we investigate the efficacy and cost effectiveness of deploying a network of wireless power line monitoring devices as a method of independently monitoring key parts of the power grid as a complement to the data which is currently available to federal agencies from grid system operators. Such a network is modeled on proprietary power line monitoring technologies and networks invented, developed and deployed by Genscape, a Louisville, Kentucky based real-time energy information provider. Genscape measures transmission line power flow using measurements of electromagnetic fields under overhead high voltage transmission power lines in the United States and Europe. Opportunities for optimization of the commercial power line monitoring technology were investigated in this project to enable lower power consumption, lower cost and improvements to measurement methodologies. These optimizations were performed in order to better enable the use of wireless transmission line monitors in large network deployments (perhaps covering several thousand power lines) for federal situational awareness needs. Power consumption and cost reduction were addressed by developing a power line monitor using a low power, low cost wireless telemetry platform known as the ''Mote''. Motes were first developed as smart sensor nodes in wireless mesh networking applications. On such a platform, it has been demonstrated in this project that wireless monitoring units can effectively deliver real-time transmission line power flow information for less than $500 per monitor. The data delivered by such a monitor has during the course of the project been integrated with a national grid situational awareness visualization platform developed by Oak Ridge National Laboratory. Novel vibration energy scavenging methods based on piezoelectric cantilevers were also developed as a proposed method to power such monitors, with a goal of further cost reduction and large-scale deployment. Scavenging methods developed during the project resulted in 50% greater power output than conventional cantilever-based vibrational energy scavenging devices typically used to power smart sensor nodes. Lastly, enhanced and new methods for electromagnetic field sensing using multi-axis magnetometers and infrared reflectometry were investigated for potential monitoring applications in situations with a high density of power lines or high levels of background 60 Hz noise in order to isolate power lines of interest from other power lines in close proximity. The goal of this project was to investigate and demonstrate the feasibility of using small form factor, highly optimized, low cost, low power, non-contact, wireless electric transmission line monitors for delivery of real-time, independent power line monitoring for the US power grid. The project was divided into three main types of activity as follows; (1) Research into expanding the range of applications for non-contact power line monitoring to enable large scale low cost sensor network deployments (Tasks 1, 2); (2) Optimization of individual sensor hardware components to reduce size, cost and power consumption and testing in a pilot field study (Tasks 3,5); and (3) Demonstration of the feasibility of using the data from the network of power line monitors via a range of custom developed alerting and data visualization applications to deliver real-time information to federal agencies and others tasked with grid reliability (Tasks 6,8).

  15. Federal Line Management Oversight of Department of Energy Nuclear...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    efficiently implementing the requirements of DOE O 226.1B, Implementation of Department of Energy Oversight Policy, dated April 25, 2011, as applied to Federal line management of ...

  16. Microsoft Word - News Release -- Annual Reports now on line 2...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    5 15 BONNEVILLE POWER ADMINISTRATION Friday, Feb. 20, 2015 CONTACT: Mike Hansen, BPA 503-230-4328 or 503-230-5131 All 77 of BPA's annual reports now on line Portland, Ore. - The...

  17. To: Mansueti, Lawrence Subject: RE: Pepco Scheduled Line Repair...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Dec. 1-20, 2006 Larry, Regarding the planned line outage, scheduled for Feb. 20 through Mar. 6, I am requesting that the residents near the plant be moved for the duration of the...

  18. Glosten Associates Tests Innovative High-Strength Mooring Lines for

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Deepwater Offshore Wind Installations | Department of Energy Glosten Associates Tests Innovative High-Strength Mooring Lines for Deepwater Offshore Wind Installations Glosten Associates Tests Innovative High-Strength Mooring Lines for Deepwater Offshore Wind Installations October 1, 2012 - 12:52pm Addthis This is an excerpt from the Third Quarter 2012 edition of the Wind Program R&D Newsletter. Funded by the U.S. Department of Energy's Wind Program, Glosten Associates recently completed

  19. The 2mrad Crossing Angle Interaction Region and Extraction Line

    SciTech Connect (OSTI)

    Appleby, R.; U., Manchester; Angal-Kalinin, D.; Dadoun, O.; Bambade, P.; Parker, B.; Keller, L.; Moffeit, K.; Nosochkov, Y.; Seryi, A.; Spencer, C.; Carter, J.; Royal Holloway, U.of London; Napoly, O.; /DAPNIA, Saclay

    2006-07-12

    A complete optics design for the 2mrad crossing angle interaction region and extraction line was presented at Snowmass 2005. Since this time, the design task force has been working on developing and improving the performance of the extraction line. The work has focused on optimizing the final doublet parameters and on reducing the power losses resulting from the disrupted beam transport. In this paper, the most recent status of the 2mrad layout and the corresponding performance are presented.

  20. Gas insulated transmission line having tapered particle trapping ring

    DOE Patents [OSTI]

    Cookson, Alan H. (Pittsburgh, PA)

    1982-01-01

    A gas-insulated transmission line includes an outer sheath, an inner conductor, insulating supports and an insulating gas. A particle-trapping ring is secured to each insulating support, and it is comprised of a central portion and two tapered end portions. The ends of the particle trapping ring have a smaller diameter than the central portion of the ring, so as to enable the use of the particle trapping ring in a curved transmission line.

  1. Part 1: The Bottom Line | Department of Energy

    Office of Environmental Management (EM)

    1: The Bottom Line Part 1: The Bottom Line Question: What is different about decommissioning under CERCLA from the way DOE has decommissioned facilities in the past? Answer: DOE will need to document and communicate decisions in a manner more in tune with regulator and stakeholder needs. Public participation requirements will be new and documents will have new formats and names. National Environmental Policy Act (NEPA) values will be incorporated in CERCLA documents. For CERCLA actions, if an

  2. Device For Trapping Laser Pulses In An Optical Delay Line

    DOE Patents [OSTI]

    Yu, David U. L. (Rancho Palos Verdes, CA); Bullock, Donald L. (Los Angeles, CA)

    1997-12-23

    A device for maintaining a high-energy laser pulse within a recirculating optical delay line for a period time to optimize the interaction of the pulse with an electron beam pulse train comprising closely spaced electron micropulses. The delay line allows a single optical pulse to interact with many of the electron micropulses in a single electron beam macropulse in sequence and for the introduction of additional optical pulses to interact with the micropulses of additional electron beam macropulses. The device comprises a polarization-sensitive beam splitter for admitting an optical pulse to and ejecting it from the delay line according to its polarization state, a Pockels cell to control the polarization of the pulse within the delay line for the purpose of maintaining it within the delay line or ejecting it from the delay line, a pair of focusing mirrors positioned so that a collimated incoming optical pulse is focused by one of them to a focal point where the pulse interacts with the electron beam and then afterwards the pulse is recollimated by the second focusing mirror, and a timing device which synchronizes the introduction of the laser pulse into the optical delay line with the arrival of the electron macropulse at the delay line to ensure the interaction of the laser pulse with a prescribed number of electron micropulses in sequence. In a first embodiment of the invention, the principal optical elements are mounted with their axes collinear. In a second embodiment, all principal optical elements are mounted in the configuration of a ring.

  3. Development of aerogel-lined targets for inertial confinement fusion

    Office of Scientific and Technical Information (OSTI)

    experiments (Thesis/Dissertation) | SciTech Connect Thesis/Dissertation: Development of aerogel-lined targets for inertial confinement fusion experiments Citation Details In-Document Search Title: Development of aerogel-lined targets for inertial confinement fusion experiments Authors: Braun, T Publication Date: 2013-03-05 OSTI Identifier: 1077169 Report Number(s): LLNL-TH-631353 DOE Contract Number: W-7405-ENG-48 Resource Type: Thesis/Dissertation Research Org: Lawrence Livermore National

  4. LLNL line-item construction projects Master Site Plan

    SciTech Connect (OSTI)

    1996-04-15

    This interim submittal is an updated 1996 overview of the Master Plan based on the 1995 LLNL Site Development Plan, illustrating the future land use considerations, and the locations of proposed facilities as documented through the line item development process and keyed to the summary table. The following components in addition to the line-item proposals remain key elements in the implementation strategy of the Master Plan: personnel migration, revitalization, space reduction, classified core contraction, utility systems, and environmental restoration.

  5. Federal Line Management Oversight of Department of Energy Nuclear Facilities

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2011-04-25

    The purpose of this Guide is to provide U.S. Department of Energy (DOE) line management with guidance that may be useful to them in effectively and efficiently implementing the requirements of DOE O 226.1B, Implementation of Department of Energy Oversight Policy, date April 25, 2011, as applied to Federal line management of hazard category 1, 2, and 3 nuclear facilities.

  6. Corrugated outer sheath gas-insulated transmission line

    DOE Patents [OSTI]

    Kemeny, George A. (Pittsburgh, PA); Cookson, Alan H. (Churchill Boro, PA)

    1981-01-01

    A gas-insulated transmission line includes two transmission line sections each of which are formed of a corrugated outer housing enclosing an inner high-voltage conductor disposed therein, with insulating support means supporting the inner conductor within the outer housing and an insulating gas providing electrical insulation therebetween. The outer housings in each section have smooth end sections at the longitudinal ends thereof which are joined together by joining means which provide for a sealing fixed joint.

  7. EIS-0483: Estes to Flatiron Substation Transmission Lines Rebuild Project,

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Larimer County, Colorado | Department of Energy 83: Estes to Flatiron Substation Transmission Lines Rebuild Project, Larimer County, Colorado EIS-0483: Estes to Flatiron Substation Transmission Lines Rebuild Project, Larimer County, Colorado SUMMARY Western Area Power Administration (Western) - with USDA Forest Service, Arapaho and Roosevelt National Forest, as a cooperating agency - is preparing an EIS that analyzes the potential environmental impacts of a proposal to rebuild and upgrade

  8. EIS-0507: Boardman-Hemingway Transmission Line, Oregon and Idaho

    Broader source: Energy.gov [DOE]

    The Bureau of Land Management and the U.S. Forest Service are preparing, with DOE’s Bonneville Power Administration (BPA) as a cooperating agency, an EIS that evaluates the potential environmental impacts of a proposal to construct about 305 miles of 500-kV transmission line from northeast Oregon to southwest Idaho. BPA’s proposed action is to partially fund part the transmission line.

  9. Northern Pass Transmission Line Project Environmental Impact Statement:

    Energy Savers [EERE]

    Announcement of Change in Public Meeting Location: Federal Register Notice Volume 78, No. 181 - September 18, 2013 | Department of Energy Northern Pass Transmission Line Project Environmental Impact Statement: Announcement of Change in Public Meeting Location: Federal Register Notice Volume 78, No. 181 - September 18, 2013 Northern Pass Transmission Line Project Environmental Impact Statement: Announcement of Change in Public Meeting Location: Federal Register Notice Volume 78, No. 181 -

  10. EcoCAR Reaches the Finish Line | Department of Energy

    Office of Environmental Management (EM)

    Reaches the Finish Line EcoCAR Reaches the Finish Line June 21, 2011 - 2:09pm Addthis The EcoCAR Challenege is a competition that challenges participating students from across North America to re-engineer a vehicle donated by General Motors. With the goal of minimizing the vehicle's fuel consumption and emissions, while maintaining its utility, safety and performance, teams had to find the best combination of cutting-edge technologies to meet these objectives. Shannon Brescher Shea

  11. Optical emission line monitor with background observation and cancellation

    DOE Patents [OSTI]

    Goff, D.R.; Notestein, J.E.

    1985-01-04

    A fiber optics based optical emission line monitoring system is provided in which selected spectral emission lines, such as the sodium D-line emission in coal combustion, may be detected in the presence of interferring background or blackbody radiation with emissions much greater in intensity than that of the emission line being detected. A bifurcated fiber optic light guide is adapted at the end of one branch to view the combustion light which is guided to a first bandpass filter, adapted to the common trunk end of the fiber. A portion of the light is reflected back through the common trunk portion of the fiber to a second bandpass filter adapted to the end of the other branch of the fiber. The first filter bandpass is centered at a wavelength corresponding to the emission line to be detected with a bandwidth of about three nanometers (nm). The second filter is centered at the same wavelength but having a width of about 10 nm. First and second light detectors are located to view the light passing through the first and second filters respectively. Thus, the second detector is blind to the light corresponding to the emission line of interest detected by the first detector and the difference between the two detector outputs is uniquely indicative of the intensity of only the combustion flame emission of interest. This instrument can reduce the effects of interfering blackbody radiation by greater than 20 dB.

  12. Optical emission line monitor with background observation and cancellation

    DOE Patents [OSTI]

    Goff, David R. (Star City, WV); Notestein, John E. (Morgantown, WV)

    1986-01-01

    A fiber optics based optical emission line monitoring system is provided in which selected spectral emission lines, such as the sodium D-line emission in coal combustion, may be detected in the presence of interferring background or blackbody radiation with emissions much greater in intensity than that of the emission line being detected. A bifurcated fiber optic light guide is adapted at the end of one branch to view the combustion light which is guided to a first bandpass filter, adapted to the common trunk end of the fiber. A portion of the light is reflected back through the common trunk portion of the fiber to a second bandpass filter adapted to the end of the other branch of the fiber. The first filter bandpass is centered at a wavelength corresponding to the emission line to be detected with a bandwidth of about three nanometers (nm). The second filter is centered at the same wavelength but having a width of about 10 nm. First and second light detectors are located to view the light passing through the first and second filters respectively. Thus, the second detector is blind to the light corresponding to the emission line of interest detected by the first detector and the difference between the two detector outputs is uniquely indicative of the intensity of only the combustion flame emission of interest. This instrument can reduce the effects of interferring blackbody radiation by greater than 20 dB.

  13. The effect of bright lines in environmental risk communication

    SciTech Connect (OSTI)

    Wilson, K.N.; Desvousges, W.H.; Smith, K.V.; Payne, J. )

    1993-01-01

    Bright lines in environmental risk communication refer to the specific levels at which an environmental risk becomes a serious health threat and action should be taken to mitigate its effects. This study examined the effect of bright lines'' in risk communication by emphasizing the radon exposure threshold level of 4 picocuries per liter. Specifically, the authors developed a computer-assisted interview containing bright-line versions of risk information. The bright-line version contained a range of possible radon levels, the corresponding number of estimated lung cancer cases, the relative health risk from radon compared to other health risks, and the EPA guidelines for mitigating levels above 4 picocuries in the home. The non-bright line version was identical to the bright-line version, except it did not include the EPA's mitigation recommendations. Effect measures included respondents' change in perceived risk after reading their materials, intended testing behavior, and advice to their neighbor for a specified radon level either above or below the 4 picocury threshold level. This paper discusses broader policy implications for designing effective risk communication programs.

  14. Single-Molecule Dynamics Reveals Cooperative Binding-Folding in Protein Recognition

    SciTech Connect (OSTI)

    Wang, Jin; Lu, Qiang N.; Lu, H PETER.

    2006-07-01

    The study of associations between two biomolecules is the key to understand molecular recognition and function. Molecular function is often thought to be determined by the underlying structures. Here, combining single molecule study of protein binding with an energy landscape inspired microscopic model, we found strong evidences that bio-molecular recognition is determined by flexibilities in addition to structures. Our model is based on coarse grained molecular dynamics performed on the residue level with the energy function biased towards the native binding structure (Go model). With our model, the underlying free energy landscape of the binding can be explored. Two distinct conformational states as free energy minimum, one with partially folding of CBD and significant binding of CBD to CDC42, and another with native folding of CBD and native binding of CBD to CDC42, are clearly seen. This shows the binding process proceeds with significant interface binding of CBD with CDC42 first without complete folding of CBD. Finally binding and folding are coupled with each other cooperatively to reach the native binding state. The single molecule experimental finding of the dynamic fluctuations between the loosely bound and closely bound conformational states can be identified with theoretically calculated free energy minimum and quantitatively explained in our model as a result of binding associated with large conformational changes. Theoretical predictions have identified certain key residues for binding which are consistent with mutational experiments. The combined study provides a test ground for fundamental mechanisms as well as insights into design and further explorations on biomolecular recognition with large conformational changes.

  15. Investigation Of In-Line Monitoring Options At H Canyon/HB Line For Plutonium Oxide Production

    SciTech Connect (OSTI)

    Sexton, L.

    2015-10-14

    H Canyon and HB Line have a production goal of 1 MT per year of plutonium oxide feedstock for the MOX facility by FY17 (AFS-2 mission). In order to meet this goal, steps will need to be taken to improve processing efficiency. One concept for achieving this goal is to implement in-line process monitoring at key measurement points within the facilities. In-line monitoring during operations has the potential to increase throughput and efficiency while reducing costs associated with laboratory sample analysis. In the work reported here, we mapped the plutonium oxide process, identified key measurement points, investigated alternate technologies that could be used for in-line analysis, and initiated a throughput benefit analysis.

  16. Expression of low-, intermediate-, and high-affinity IL-2 receptors on B cell lines derived from patients with undifferentiated lymphoma of Burkitt's and non-Burkitt's types

    SciTech Connect (OSTI)

    Benjamin, D.; Rosolen, A.; Wormsley, S.B.; DeBault, L.E.; Colamonici, O.R. )

    1990-08-01

    IL-2 receptors on T cells exist in at least three forms which differ in their ligand-binding affinity. The low-affinity IL-2 receptor (IL-2R) consists of the 55-kDa Tac protein (p55 alpha), the intermediate-affinity site corresponds to the 70-kDa molecule (p70 beta), and the high-affinity IL-2R consists of a noncovalent heterodimeric structure involving both p55 alpha and p70 beta. We studied 24 B cell lines (8 EBV-negative and 16 EBV-positive) for IL-2R expression in the presence or absence of the tumor promoter, teleocidin. 125I-IL-2 radioreceptor binding assays and crosslinking studies demonstrated the sole expression of p55 alpha in EBV-negative cell lines only, whereas p55 alpha present in EBV-positive cell lines was always associated with p70 beta to construct high-affinity IL-2R. p70 beta was not detected in any of the EBV-negative cell lines, but was expressed on most of the EBV-positive cell lines (13 of 16). Our data also indicate that the expression of p55 alpha and p70 beta by radiolabeling correlates with their expression in flow cytometry, and that a large excess of p55 alpha is required to construct high-affinity IL-2R. Coexpression of p55 alpha and p70 beta on human B cells contributed to constructing high-affinity IL-2R hybrid complex as shown by rapid association rate contributed by p55 alpha and slow dissociation rate by p70 beta; teleocidin's ability to induce p55 alpha on cell lines which express p70 beta only, resulting in appearance of high-affinity IL-2R; and blocking p55 alpha by anti-Tac mAb in cell lines which constitutively express high-affinity IL-2R eliminated both high- and low-affinity components. The existence of low, intermediate, and high IL-2R on human B cells bears important future implications for understanding the mechanism of IL-2 signaling and the role of IL-2 in B cell activation, proliferation, and differentiation.

  17. HB-Line Plutonium Oxide Data Collection Strategy

    SciTech Connect (OSTI)

    Watkins, R.; Varble, J.; Jordan, J.

    2015-05-26

    HB-Line and H-Canyon will handle and process plutonium material to produce plutonium oxide for feed to the Mixed Oxide Fuel Fabrication Facility (MFFF). However, the plutonium oxide product will not be transferred to the MFFF directly from HB-Line until it is packaged into a qualified DOE-STD-3013-2012 container. In the interim, HB-Line will load plutonium oxide into an inner, filtered can. The inner can will be placed in a filtered bag, which will be loaded into a filtered outer can. The outer can will be loaded into a certified 9975 with getter assembly in compliance with onsite transportation requirement, for subsequent storage and transfer to the K-Area Complex (KAC). After DOE-STD-3013-2012 container packaging capabilities are established, the product will be returned to HB-Line to be packaged into a qualified DOE-STD-3013-2012 container. To support the transfer of plutonium oxide to KAC and then eventually to MFFF, various material and packaging data will have to be collected and retained. In addition, data from initial HB-Line processing operations will be needed to support future DOE-STD-3013-2012 qualification as amended by the HB-Line DOE Standard equivalency. As production increases, the volume of data to collect will increase. The HB-Line data collected will be in the form of paper copies and electronic media. Paper copy data will, at a minimum, consist of facility procedures, nonconformance reports (NCRs), and DCS print outs. Electronic data will be in the form of Adobe portable document formats (PDFs). Collecting all the required data for each plutonium oxide can will be no small effort for HB-Line, and will become more challenging once the maximum annual oxide production throughput is achieved due to the sheer volume of data to be collected. The majority of the data collected will be in the form of facility procedures, DCS print outs, and laboratory results. To facilitate complete collection of this data, a traveler form will be developed which identifies the required facility procedures, DCS print outs, and laboratory results needed to assemble a final data package for each HB-Line plutonium oxide interim oxide can. The data traveler may identify the specific values (data) required to be extracted from the collected facility procedures and DCS print outs. The data traveler may also identify associated criteria to be checked. Inevitably there will be procedure anomalies during the course of the HB-Line plutonium oxide campaign that will have to be addressed in a timely manner.

  18. Discriminating binding mechanisms of an intrinsically disordered protein via a multi-state coarse-grained model

    SciTech Connect (OSTI)

    Knott, Michael [Department of Chemistry, Cambridge University, Lensfield Road, Cambridge CB2 1EW (United Kingdom)] [Department of Chemistry, Cambridge University, Lensfield Road, Cambridge CB2 1EW (United Kingdom); Best, Robert B., E-mail: robertbe@helix.nih.gov [Department of Chemistry, Cambridge University, Lensfield Road, Cambridge CB2 1EW (United Kingdom); Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892-0520 (United States)

    2014-05-07

    Many proteins undergo a conformational transition upon binding to their cognate binding partner, with intrinsically disordered proteins (IDPs) providing an extreme example in which a folding transition occurs. However, it is often not clear whether this occurs via an “induced fit” or “conformational selection” mechanism, or via some intermediate scenario. In the first case, transient encounters with the binding partner favour transitions to the bound structure before the two proteins dissociate, while in the second the bound structure must be selected from a subset of unbound structures which are in the correct state for binding, because transient encounters of the incorrect conformation with the binding partner are most likely to result in dissociation. A particularly interesting situation involves those intrinsically disordered proteins which can bind to different binding partners in different conformations. We have devised a multi-state coarse-grained simulation model which is able to capture the binding of IDPs in alternate conformations, and by applying it to the binding of nuclear coactivator binding domain (NCBD) to either ACTR or IRF-3 we are able to determine the binding mechanism. By all measures, the binding of NCBD to either binding partner appears to occur via an induced fit mechanism. Nonetheless, we also show how a scenario closer to conformational selection could arise by choosing an alternative non-binding structure for NCBD.

  19. Distinct p53 genomic binding patterns in normal and cancer-derived human cells

    SciTech Connect (OSTI)

    Botcheva K.; McCorkle S. R.; McCombie W. R.; Dunn J. J.; Anderson C. W.

    2011-12-15

    We report here genome-wide analysis of the tumor suppressor p53 binding sites in normal human cells. 743 high-confidence ChIP-seq peaks representing putative genomic binding sites were identified in normal IMR90 fibroblasts using a reference chromatin sample. More than 40% were located within 2 kb of a transcription start site (TSS), a distribution similar to that documented for individually studied, functional p53 binding sites and, to date, not observed by previous p53 genome-wide studies. Nearly half of the high-confidence binding sites in the IMR90 cells reside in CpG islands, in marked contrast to sites reported in cancer-derived cells. The distinct genomic features of the IMR90 binding sites do not reflect a distinct preference for specific sequences, since the de novo developed p53 motif based on our study is similar to those reported by genome-wide studies of cancer cells. More likely, the different chromatin landscape in normal, compared with cancer-derived cells, influences p53 binding via modulating availability of the sites. We compared the IMR90 ChIPseq peaks to the recently published IMR90 methylome1 and demonstrated that they are enriched at hypomethylated DNA. Our study represents the first genome-wide, de novo mapping of p53 binding sites in normal human cells and reveals that p53 binding sites reside in distinct genomic landscapes in normal and cancer-derived human cells.

  20. Laboratory Measurements of White Dwarf Photospheric Spectral Lines. H?

    SciTech Connect (OSTI)

    Falcon, Ross Edward; Rochau, Gregory A.; Bailey, James E.; Gomez, Thomas; Montgomery, Michael Houston; Winget, Donald E; Nagayama, Taisuke

    2015-06-18

    We spectroscopically measure multiple hydrogen Balmer line profiles from laboratory plasmas to investigate the theoretical line profiles used in white dwarf (WD) atmosphere models. X-ray radiation produced at the Z Pulsed Power Facility at Sandia National Laboratories initiates plasma formation in a hydrogen-filled gas cell, replicating WD photospheric conditions. We also present time-resolved measurements of H? and fit this line using different theoretical line profiles to diagnose electron density, ne, and n = 2 level population, n2. Aided by synthetic tests, we characterize the validity of our diagnostic method for this experimental platform. During a single experiment, we infer a continuous range of electron densities increasing from ne ~ 4 to ~30 × 1016 cm-3 throughout a 120-ns evolution of our plasma. Also, we observe n2 to be initially elevated with respect to local thermodynamic equilibrium (LTE); it then equilibrates within ~55 ns to become consistent with LTE. This also supports our electron-temperature determination of Te ~ 1.3 eV (~15,000 K) after this time. At ne? 1017 cm-3, we find that computer-simulation-based line-profile calculations provide better fits (lower reduced ?2) than the line profiles currently used in the WD astronomy community. The inferred conditions, however, are in good quantitative agreement. This work establishes an experimental foundation for the future investigation of relative shapes and strengths between different hydrogen Balmer lines.

  1. Expression, purification, crystallization and structure of human adipocyte lipid-binding protein (aP2)

    SciTech Connect (OSTI)

    Marr, Eric; Tardie, Mark; Carty, Maynard; Brown Phillips, Tracy; Wang, Ing-Kae; Soeller, Walt; Qiu, Xiayang Karam, George

    2006-11-01

    The crystal structure of human adipocyte lipid-binding protein (aP2) with a bound palmitate is reported at 1.5 Å resolution. Human adipocyte lipid-binding protein (aP2) belongs to a family of intracellular lipid-binding proteins involved in the transport and storage of lipids. Here, the crystal structure of human aP2 with a bound palmitate is described at 1.5 Å resolution. Unlike the known crystal structure of murine aP2 in complex with palmitate, this structure shows that the fatty acid is in a folded conformation and that the loop containing Phe57 acts as a lid to regulate ligand binding by excluding solvent exposure to the central binding cavity.

  2. HIGH-n HYDROGEN RECOMBINATION LINES FROM THE FIRST GALAXIES

    SciTech Connect (OSTI)

    Rule, E.; Loeb, A.; Strelnitski, V. S.

    2013-09-20

    We investigate the prospects of blind and targeted searches in the radio domain (10 MHz to 1 THz) for high-n hydrogen recombination lines from the first generation of galaxies, at z ?< 10. The expected optically thin spontaneous ?-line luminosities are calculated as a function of the absolute AB magnitude of a galaxy at 1500 Å. For a blind search, semi-empirical luminosity functions are used to calculate the number of galaxies whose expected flux densities exceed an assumed detectability threshold. Plots of the minimum sky area, within which at least one detectable galaxy is expected at a given observing frequency, in the fiducial instantaneous passband of 10{sup 4} km s{sup –1}, allow us to assess the blind search time necessary for detection by a given facility. We show that the chances for detection are the highest in the millimeter and submillimeter domains, but finding spontaneous emission in a blind search, especially from redshifts z >> 1, is a challenge even with powerful facilities, such as the Actama Large Millimeter/Submillimeter Array and Square Kilometre Array. The probability of success is higher for a targeted search of lines with principal quantum number n ? 10 in Lyman-break galaxies amplified by gravitational lensing. Detection of more than one hydrogen line in such a galaxy will allow for line identification and a precise determination of the galaxy's redshift.

  3. LineVISAR. A fringe-trace data analysis program

    SciTech Connect (OSTI)

    Furnish, Michael D.

    2014-02-01

    The line-imaging ORVIS or VISAR provides velocity as a function of position and time for a line on an experimental setup via a streak camera record of interference fringes. This document describes a Matlab-based program which guides the user through the process of converting these fringe data to a velocity surface. The data reduction is of the "fringe trace" type, wherein the changes in velocity at a given position on the line are calculated based on fringe motion past that point. The analyst must establish the fringe behavior up front, aided by peak-finding routines in the program. However, the later work of using fringe jumps to compensate for phase problems in other analysis techniques is greatly reduced. This program is not a standard GUI construction, and is prescriptive. At various points it saves the progress, allowing later restarts from those points.

  4. CONTINUOUS EXTRACTED BEAM IN THE AGS FAST EXTERNAL BEAM LINE.

    SciTech Connect (OSTI)

    GLENN,J.W.; TSOUPAS,N.; BROWN,K.A.; BIRYUKOV,V.M.

    2001-06-18

    A method to split off a few percent of the 6 x 10{sup 13} AGS beam delivered to the Slow External Beam (SEB) lines and send it down the Fast External Beam line (FEB) has been developed. The mission is to feed a counter experiment off the FEB that directly measures the neutrino mass using the muon storage ring. The use of normal thin septum splitters would have an excessive loss overhead and been optically difficult. The AGS Slow Extraction uses a third integer resonance with sextuple strength so the resonance width is a few percent of the beam width. This results in a low density tail which will be clipped by a bent crystal and deflected into the FEB channel. This clipping off of the tail should reduce losses in the SEB transport line. Details of modeled orbits, particle distribution and extraction trajectories into and out off the crystal will be given.

  5. Electric and magnetic field reduction by alternative transmission line options

    SciTech Connect (OSTI)

    Stewart, J.R. (Power Technologies, Inc., Schenectady, NY (United States)); Dale, S.J. (Oak Ridge National Lab., TN (United States)); Klein, K.W. (Energetics, Inc., Columbia, MD (United States))

    1991-01-01

    Ground level electric, and more recently magnetic, fields from overhead power transmission lines are increasingly important considerations in right of way specification, with states setting or planning to set edge of right of way limits. Research has been conducted in high phase order power transmission wherein six of twelve phases are used to transmit power in less physical space and with reduced electrical environmental effects than conventional designs. The first magnetic field testing, as reported in this paper, has verified predictive methods for determination of magnetic fields from high phase order lines. Based on these analytical methods, field profiles have been determined for lines of different phase order of comparable power capacity. Potential advantages of high phase order as a means of field mitigation are discussed. 10 refs., 12 figs., 3 tabs.

  6. Phase Transitions above the Yrast Line in {sup 154}Dy

    SciTech Connect (OSTI)

    Ma, W. C.; Martin, V.; Khoo, T. L.; Lauritsen, T.; Egido, J. L.; Ahmad, I.; Bhattacharyya, P.; Carpenter, M. P.; Daly, P. J.; Grabowski, Z. W.

    2000-06-26

    Spectra of the E2 quasicontinuum {gamma} rays feeding different spin regions of the {sup 154}Dy yrast line have been extracted. These are compared with corresponding theoretical spectra obtained by numerical simulations based on temperature-dependent Hartree-Fock theory, with thermal shape fluctuations. In this manner, different regions of the spin-energy plane can be examined. The results support the predictions of a smeared-out phase transition at high spin above the yrast line. (c) 2000 The American Physical Society.

  7. Evaluation of Instrumentation and Dynamic Thermal Ratings for Overhead Lines

    SciTech Connect (OSTI)

    Phillips, A.

    2013-01-31

    In 2010, a project was initiated through a partnership between the Department of Energy (DOE) and the New York Power Authority (NYPA) to evaluate EPRI?s rating technology and instrumentation that can be used to monitor the thermal states of transmission lines and provide the required real-time data for real-time rating calculations. The project included the installation and maintenance of various instruments at three 230 kV line sites in northern New York. The instruments were monitored, and data collection and rating calculations were performed for about a three year period.

  8. Self-monitoring high voltage transmission line suspension insulator

    DOE Patents [OSTI]

    Stemler, Gary E. (Vancouver, WA); Scott, Donald N. (Vancouver, WA)

    1981-01-01

    A high voltage transmission line suspension insulator (18 or 22) which monitors its own dielectric integrity. A dielectric rod (10) has one larger diameter end fitting attachable to a transmission line and another larger diameter end fitting attachable to a support tower. The rod is enclosed in a dielectric tube (14) which is hermetically sealed to the rod's end fittings such that a liquidtight space (20) is formed between the rod and the tube. A pressurized dielectric liquid is placed within that space. A discoloring dye placed within this space is used to detect the loss of the pressurized liquid.

  9. Cast-stone sectors for lining bends in pipework

    SciTech Connect (OSTI)

    Chechulin, V.A.; Novikov, A.I.; Karpov, V.M.; Sotnik, A.A.; Sedyshev, B.L.

    1987-03-01

    The authors disclose an efficient method for lining the bends of pipelines used to deliver coal dust to the burners of coal-fired power plants or to transport coal slurries in mining and preparation enterprises. The method consists of melting a wear-resistant silicate compound and casting it in the form of rings whose increased width on the outboard side accounts for the angle of the bend when the rings are installed consecutively inside the pipe. Enhanced service life estimations and cost benefit analyses are given for pipe bends thus lined in both of the above applications.

  10. Dynamic Line Rating Oncor Electric Delivery Smart Grid Program

    SciTech Connect (OSTI)

    Johnson, Justin; Smith, Cale; Young, Mike; Donohoo, Ken; Owen, Ross; Clark, Eddit; Espejo, Raul; Aivaliotis, Sandy; Stelmak, Ron; Mohr, Ron; Barba, Cristian; Gonzalez, Guillermo; Malkin, Stuart; Dimitrova, Vessela; Ragsdale, Gary; Mitchem, Sean; Jeirath, Nakul; Loomis, Joe; Trevino, Gerardo; Syracuse, Steve; Hurst, Neil; Mereness, Matt; Johnson, Chad; Bivens, Carrie

    2013-05-04

    Electric transmission lines are the lifeline of the electric utility industry, delivering its product from source to consumer. This critical infrastructure is often constrained such that there is inadequate capacity on existing transmission lines to efficiently deliver the power to meet demand in certain areas or to transport energy from high-generation areas to high-consumption regions. When this happens, the cost of the energy rises; more costly sources of power are used to meet the demand or the system operates less reliably. These economic impacts are known as congestion, and they can amount to substantial dollars for any time frame of reference: hour, day or year. There are several solutions to the transmission constraint problem, including: construction of new generation, construction of new transmission facilities, rebuilding and reconductoring of existing transmission assets, and Dynamic Line Rating (DLR). All of these options except DLR are capital intensive, have long lead times and often experience strong public and regulatory opposition. The Smart Grid Demonstration Program (SGDP) project co-funded by the Department of Energy (DOE) and Oncor Electric Delivery Company developed and deployed the most extensive and advanced DLR installation to demonstrate that DLR technology is capable of resolving many transmission capacity constraint problems with a system that is reliable, safe and very cost competitive. The SGDP DLR deployment is the first application of DLR technology to feed transmission line real-time dynamic ratings directly into the system operation’s State Estimator and load dispatch program, which optimizes the matching of generation with load demand on a security, reliability and economic basis. The integrated Dynamic Line Rating (iDLR)1 collects transmission line parameters at remote locations on the lines, calculates the real-time line rating based on the equivalent conductor temperature, ambient temperature and influence of wind and solar radiation on the stringing section, transmits the data to the Transmission Energy Management System, validates its integrity and passes it on to Oncor and ERCOT (Electric Reliability Council of Texas) respective system operations. The iDLR system is automatic and transparent to ERCOT System Operations, i.e., it operates in parallel with all other system status telemetry collected through Supervisory Control and Data Acquisition (SCADA) employed across the company.

  11. SiteLines Looks at NNSS Successes in 2011

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    SiteLines Looks at NNSS Successes in 2011 It was a big year at the Nevada National Security Site (NNSS) for the Nevada Site Office and its contractors. From returning to nuclear operations at the Joint Actinide Shock Physics Experimental Research (JASPER) facility to the formation of the National Center for Nuclear Security to the implementation of Governance Reform, the NNSS had one of its most successful years in FY2011. Now, with its sights set on 2012, SiteLines takes a closer look at the

  12. Characterization Plan for Soils Around Drain Line PLA-100115

    SciTech Connect (OSTI)

    D. Shanklin

    2006-05-24

    This Characterization Plan supports the Hazardous Waste Management Act/Resource Conservation and Recovery Act (HWMA/RCRA) closure of soils that may have been contaminated by releases from drain line PLA-100115, located within the Idaho Nuclear Technology and Engineering Center at the Idaho National Laboratory Site. The requirements to address the closure of soils contaminated by a potential release from this line in a characterization plan was identified in the "HWMA/RCRA Less Than 90-day Generator Closure Report for the VES-SFE-126."

  13. Erratum: ''Titanium and germanium lined hohlraums and halfraums as

    Office of Scientific and Technical Information (OSTI)

    multi-keV x-ray radiators'' [Phys. Plasmas 16, 052704 (2009)] (Journal Article) | SciTech Connect Erratum: ''Titanium and germanium lined hohlraums and halfraums as multi-keV x-ray radiators'' [Phys. Plasmas 16, 052704 (2009)] Citation Details In-Document Search Title: Erratum: ''Titanium and germanium lined hohlraums and halfraums as multi-keV x-ray radiators'' [Phys. Plasmas 16, 052704 (2009)] No abstract prepared. Authors: Girard, F. ; Primout, M. ; Villette, B. ; Stemmler, Ph. ; Jacquet,

  14. DOE - NNSA/NSO -- SiteLines Web Edition

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Calendar NTS Public Tours Factsheets Speakers Bureau Acronyms Mailing List Information Request Publishing Information Questions/Contact Masthead Archives FAQs/QUESTIONS SUBJECT INDEX SITEMAP SEARCH CONTACT US ABOUT US HELP ACRONYMS FOIA PRIVACY ACT WEBSITE POLICIES HOMEPAGE Home > Library > SiteLines U.S. DOE/NNSA - Nevada Site Office December 2007/January 2008 - Issue 129 A publication for all members of the NNSA/NSO family SiteLines looks back at 2007 2007 was a busy and productive year

  15. Rapidity evolution of Wilson lines at the next-to-leading order

    SciTech Connect (OSTI)

    Balitsky, Ian; Chirilli, Giovanni

    2013-12-01

    At high energies particles move very fast so the proper degrees of freedom for the fast gluons moving along the straight lines are Wilson-line operators - infinite gauge factors ordered along the line. In the framework of operator expansion in Wilson lines the energy dependence of the amplitudes is determined by the rapidity evolution of Wilson lines. We present the next-to-leading order hierarchy of the evolution equations for Wilson-line operators.

  16. Plains & Eastern Clean Line Project Proposal for New or Upgraded

    Energy Savers [EERE]

    Transmission Line Projects Under Section 1222 of the Energy Policy Act of 2005 | Department of Energy Project Proposal for New or Upgraded Transmission Line Projects Under Section 1222 of the Energy Policy Act of 2005 Plains & Eastern Clean Line Project Proposal for New or Upgraded Transmission Line Projects Under Section 1222 of the Energy Policy Act of 2005 PDF icon Plains & Eastern Clean Line Project Proposal for New or Upgraded Transmission Line Projects Under Section 1222 of the

  17. Letter from Deputy Secretary Poneman to Clean Line Energy Regarding the

    Office of Environmental Management (EM)

    Plains & Eastern Clean Line Project Under Section 1222 of EPAct 2005 (April 5, 2012) | Department of Energy Letter from Deputy Secretary Poneman to Clean Line Energy Regarding the Plains & Eastern Clean Line Project Under Section 1222 of EPAct 2005 (April 5, 2012) Letter from Deputy Secretary Poneman to Clean Line Energy Regarding the Plains & Eastern Clean Line Project Under Section 1222 of EPAct 2005 (April 5, 2012) PDF icon Letter from Deputy Secretary Poneman to Clean Line

  18. NMR studies of DNA oligomers and their interactions with minor groove binding ligands

    SciTech Connect (OSTI)

    Fagan, P A

    1996-05-01

    The cationic peptide ligands distamycin and netropsin bind noncovalently to the minor groove of DNA. The binding site, orientation, stoichiometry, and qualitative affinity of distamycin binding to several short DNA oligomers were investigated by NMR spectroscopy. The oligomers studied contain A,T-rich or I,C-rich binding sites, where I = 2-desaminodeoxyguanosine. I{center_dot}C base pairs are functional analogs of A{center_dot}T base pairs in the minor groove. The different behaviors exhibited by distamycin and netropsin binding to various DNA sequences suggested that these ligands are sensitive probes of DNA structure. For sites of five or more base pairs, distamycin can form 1:1 or 2:1 ligand:DNA complexes. Cooperativity in distamycin binding is low in sites such as AAAAA which has narrow minor grooves, and is higher in sites with wider minor grooves such as ATATAT. The distamycin binding and base pair opening lifetimes of I,C-containing DNA oligomers suggest that the I,C minor groove is structurally different from the A,T minor groove. Molecules which direct chemistry to a specific DNA sequence could be used as antiviral compounds, diagnostic probes, or molecular biology tools. The author studied two ligands in which reactive groups were tethered to a distamycin to increase the sequence specificity of the reactive agent.

  19. Assessing Energetic Contributions to Binding from a Disordered Region in a Protein-Protein Interaction

    SciTech Connect (OSTI)

    S Cho; C Swaminathan; D Bonsor; M Kerzic; R Guan; J Yang; C Kieke; P Anderson; D Kranz; et al.

    2011-12-31

    Many functional proteins are at least partially disordered prior to binding. Although the structural transitions upon binding of disordered protein regions can influence the affinity and specificity of protein complexes, their precise energetic contributions to binding are unknown. Here, we use a model protein-protein interaction system in which a locally disordered region has been modified by directed evolution to quantitatively assess the thermodynamic and structural contributions to binding of disorder-to-order transitions. Through X-ray structure determination of the protein binding partners before and after complex formation and isothermal titration calorimetry of the interactions, we observe a correlation between protein ordering and binding affinity for complexes along this affinity maturation pathway. Additionally, we show that discrepancies between observed and calculated heat capacities based on buried surface area changes in the protein complexes can be explained largely by heat capacity changes that would result solely from folding the locally disordered region. Previously developed algorithms for predicting binding energies of protein-protein interactions, however, are unable to correctly model the energetic contributions of the structural transitions in our model system. While this highlights the shortcomings of current computational methods in modeling conformational flexibility, it suggests that the experimental methods used here could provide training sets of molecular interactions for improving these algorithms and further rationalizing molecular recognition in protein-protein interactions.

  20. WAC - 468-34 - Utility Lines-Franchises and Permits | Open Energy...

    Open Energy Info (EERE)

    Lines-Franchises and PermitsLegal Abstract This section outlines the rules and procedures for granting franchises and permits for utility lines in the state. Published NA...

  1. EA-1972: Electric District 2 to Saguaro No. 2 Transmission Line...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    2: Electric District 2 to Saguaro No. 2 Transmission Line Rebuild; Pinal County, Arizona EA-1972: Electric District 2 to Saguaro No. 2 Transmission Line Rebuild; Pinal County, ...

  2. Method for detecting binding events using micro-X-ray fluorescence spectrometry

    DOE Patents [OSTI]

    Warner, Benjamin P. (Los Alamos, NM); Havrilla, George J. (Los Alamos, NM); Mann, Grace (Hong Kong, HK)

    2010-12-28

    Method for detecting binding events using micro-X-ray fluorescence spectrometry. Receptors are exposed to at least one potential binder and arrayed on a substrate support. Each member of the array is exposed to X-ray radiation. The magnitude of a detectable X-ray fluorescence signal for at least one element can be used to determine whether a binding event between a binder and a receptor has occurred, and can provide information related to the extent of binding between the binder and receptor.

  3. X-ray crystallographic analysis of adipocyte fatty acid binding protein

    Office of Scientific and Technical Information (OSTI)

    (aP2) modified with 4-hydroxy-2-nonenal (Journal Article) | SciTech Connect X-ray crystallographic analysis of adipocyte fatty acid binding protein (aP2) modified with 4-hydroxy-2-nonenal Citation Details In-Document Search Title: X-ray crystallographic analysis of adipocyte fatty acid binding protein (aP2) modified with 4-hydroxy-2-nonenal Fatty acid binding proteins (FABP) have been characterized as facilitating the intracellular solubilization and transport of long-chain fatty acyl

  4. Safety and Health Regulatory and Policy Response Line- General Information

    Broader source: Energy.gov [DOE]

    The DOE Worker Safety and Health Standards Response Line, established in 1992, is a service that responds to questions DOE, DOE contractor, and DOE subcontractor personnel regarding DOE-adopted and -prescribed standards and directives. These responses may not represent official OSHA policies.

  5. Strengthening Line Management Oversight and Federal Monitoring of Nuclear Facilities

    Office of Environmental Management (EM)

    Strengthening Line Management Oversight and Federal Monitoring of Nuclear Facilities Standard Review Plan Volume 2 -- Nuclear Safety Basis Program Review During Design February 2015 i Standard Review Plan Volume 2 Nuclear Safety Basis Program Review during Design Facility Life Cycle Applicability CD-1 CD-2 CD-3 CD-4 Operations and Transitions Decommissioning & Environmental Restoration February 2015 ii Table of Contents Acronyms

  6. SNOW LINES AS PROBES OF TURBULENT DIFFUSION IN PROTOPLANETARY DISKS

    SciTech Connect (OSTI)

    Owen, James E. [Canadian Institute for Theoretical Astrophysics, 60 St George Street, Toronto, M5S 3H8, ON (Canada)

    2014-07-20

    Sharp chemical discontinuities can occur in protoplanetary disks, particularly at ''snow lines'' where a gas-phase species freezes out to form ice grains. Such sharp discontinuities will diffuse out due to the turbulence suspected to drive angular momentum transport in accretion disks. We demonstrate that the concentration gradient—in the vicinity of the snow line—of a species present outside a snow line but destroyed inside is strongly sensitive to the level of turbulent diffusion (provided the chemical and transport timescales are decoupled) and provides a direct measurement of the radial ''Schmidt number'' (the ratio of the angular momentum transport to radial turbulent diffusion). Taking as an example the tracer species N{sub 2}H{sup +}, which is expected to be destroyed inside the CO snow line (as recently observed in TW Hya) we show that ALMA observations possess significant angular resolution to constrain the Schmidt number. Since different turbulent driving mechanisms predict different Schmidt numbers, a direct measurement of the Schmidt number in accretion disks would allow inferences to be made about the nature of the turbulence.

  7. EA-1960: Townsite Solar Project Transmission Line, Clark County, Nevada

    Broader source: Energy.gov [DOE]

    The Bureau of Land Management, with Western Area Power Administration as a cooperating agency, prepared an EA to evaluate potential impacts of a proposal to build and operate a 180-MW photovoltaic facility; a 220, 230, or 500 kV transmission line; and associated facilities in Clark County, Nevada.

  8. QER- Comment of Clean Line Energy Partners LLC

    Broader source: Energy.gov [DOE]

    Hello, Clean Line Energy Partners would like to submit the attached written comments to the QER task force, in conjunction with the verbal comments Jimmy Glotfelty provided today in Newark. Thank you for your consideration, and please let us know if you would like any additional information. Sincerely, Diana Rivera

  9. Rotating superconductor magnet for producing rotating lobed magnetic field lines

    DOE Patents [OSTI]

    Hilal, Sadek K. (Englewood Cliffs, NJ); Sampson, William B. (Bellport, NY); Leonard, Edward F. (Leonia, NJ)

    1978-01-01

    This invention provides a rotating superconductor magnet for producing a rotating lobed magnetic field, comprising a cryostat; a superconducting magnet in the cryostat having a collar for producing a lobed magnetic field having oppositely directed adjacent field lines; rotatable support means for selectively rotating the superconductor magnet; and means for energizing the superconductor magnet.

  10. A Laser-Wire System at the ATF Extraction Line

    SciTech Connect (OSTI)

    Boogert, S.T.; Blair, G.; Boorman, G.; Bosco, A.; Deacon, L.; Driouichi, C.; Karataev, P.; /Royal Holloway, U. of London; Kamps, T.; /BESSY, Berlin; Delerue, N.; Dixit, S.; Foster, B.; Gannaway, F.; Howell, D.F.; Qureshi, M.; Reichold, A.; Senanayake, R.; /Oxford U.; Aryshev, A.; Hayano, H.; Kubo, K.; Terunuma, N.; Urakawa, J.; /KEK, Tsukuba /Liverpool

    2007-02-12

    A new laser-wire (LW) system has been installed at the ATF extraction line at KEK, Tsukuba. The system aims at a micron-scale laser spot size and employs a mode-locked laser system. The purpose-built interaction chamber, light delivery optics, and lens systems are described, and the first results are presented.

  11. High voltage gas insulated transmission line with continuous particle trapping

    DOE Patents [OSTI]

    Cookson, Alan H. (Pittsburgh, PA); Dale, Steinar J. (Monroeville, PA)

    1983-01-01

    This invention provides a novel high voltage gas insulated transmission line utilizing insulating supports spaced at intervals with snap-in means for supporting a continuous trapping apparatus and said trapping apparatus having perforations and cutouts to facilitate trapping of contaminating particles and system flexibility.

  12. EIS-0124: Conrad-Shelby Transmission Line Project, Montana

    Broader source: Energy.gov [DOE]

    The U.S. Department of Energy's Western Area Power Administration developed this statement to assess the environmental impact of adding a 230 kV transmission line between Conrad and Shelby, Montana and a new substation near Shelby to update the stressed electrical transmission system.

  13. EIS-0090: Fort Peck-Havre Transmission Line Project, Montana

    Broader source: Energy.gov [DOE]

    The U.S. Department of Energy’s Western Area Power Administration prepared this statement to assess the potential environmental and socioeconomic implications of its proposed action to construct and operate a 230kV transmission line from Fort Peck to Havre, Montana, with three intermediate interconnecting substations.

  14. Gamma ray lines from a universal extra dimension

    SciTech Connect (OSTI)

    Bertone, G.; Jackson, C. B.; Shaughnessy, G.; Tait, T. M.P.; Vallinotto, A.

    2012-03-01

    Indirect Dark Matter searches are based on the observation of secondary particles produced by the annihilation or decay of Dark Matter. Among them, gamma-rays are perhaps the most promising messengers, as they do not suffer deflection or absorption on Galactic scales, so their observation would directly reveal the position and the energy spectrum of the emitting source. Here, we study the detailed gamma-ray energy spectrum of Kaluza--Klein Dark Matter in a theory with 5 Universal Extra Dimensions. We focus in particular on the two body annihilation of Dark Matter particles into a photon and another particle, which produces monochromatic photons, resulting in a line in the energy spectrum of gamma rays. Previous calculations in the context of the five dimensional UED model have computed the line signal from annihilations into \\gamma \\gamma, but we extend these results to include \\gamma Z and \\gamma H final states. We find that these spectral lines are subdominant compared to the predicted \\gamma \\gamma signal, but they would be important as follow-up signals in the event of the observation of the \\gamma \\gamma line, in order to distinguish the 5d UED model from other theoretical scenarios.

  15. Southern Asia future plans feature long-distance lines

    SciTech Connect (OSTI)

    Not Available

    1991-11-01

    This paper reports that although pipe line mileage working, planned and under study has dipped slightly from 47,346 km (29,420 mi) to 44,853 km (27,871 mi), Southern Asia continues to hold a strong position for future projects with some of the most interesting programs in the international market. Two dramatic, long-distance natural gas transmission, gathering and lateral networks continue to hold the future pipe line construction spotlight in Southern Asia. The Association of South East Asian Nations (ASEAN) continues to study a 7,830 km (4,865 mi) gas transmission system. With an estimated cost of $10 billion, the system includes some 6,276 km (3,900 mi) of transmission lines, with 1,094 km (680 mi) offshore. Group members include Brunei, Indonesia, Malaysia, the Philippines, Singapore and Thailand. The second project, the Trans-Asian Pipeline System, involves 3,380 km (2,100 mi) of transmission lines from the Iran's Bandar Abbas gas field across Pakistan to a terminal at Calcutta, India.

  16. 241-AY-102 Leak Detection Pit Drain Line Inspection Report

    SciTech Connect (OSTI)

    Boomer, Kayle D.; Engeman, Jason K.; Gunter, Jason R.; Joslyn, Cameron C.; Vazquez, Brandon J.; Venetz, Theodore J.; Garfield, John S.

    2014-01-20

    This document provides a description of the design components, operational approach, and results from the Tank AY-102 leak detection pit drain piping visual inspection. To perform this inspection a custom robotic crawler with a deployment device was designed, built, and operated by IHI Southwest Technologies, Inc. for WRPS to inspect the 6-inch leak detection pit drain line.

  17. Operating Experience Level 3, Danger! Equipment Hits Overhead Lines

    Broader source: Energy.gov [DOE]

    OE-3 2016-01: This Operating Experience Level 3 (OE-3) document provides information about a safety concern related to workers and operations at Department of Energy (DOE) facilities where equipment has struck overhead communication cables and energized power lines.

  18. Replacement of alloy 800H superheated steam line

    SciTech Connect (OSTI)

    Barbier, R.A.; Bullock, J.W. [Sterling Chemicals, Texas City, TX (United States)

    1996-07-01

    Sterling Chemicals utilizes alloy 800HT (UNS N08811) piping for superheated steam service in its styrene dehydrogenation unit. An engineering project to replace these lines was recently completed. Material acquisition, shop fabrication, inspection requirements, and field erection will be highlighted in this paper.

  19. NNSA's Second Line of Defense Program Receives Capability Award |

    National Nuclear Security Administration (NNSA)

    National Nuclear Security Administration Second Line of Defense Program Receives Capability Award | National Nuclear Security Administration Facebook Twitter Youtube Flickr RSS People Mission Managing the Stockpile Preventing Proliferation Powering the Nuclear Navy Emergency Response Recapitalizing Our Infrastructure Countering Nuclear Terrorism About Our Programs Our History Who We Are Our Leadership Our Locations Budget Our Operations Library Bios Congressional Testimony Fact Sheets

  20. Federal Line Management Oversight of Department of Energy Nuclear Facilities

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2014-04-14

    The Guide was developed in support of DOE O 226.1B to provide guidance that may be useful to DOE line management organizations in meeting the provisions of that order when applied to nuclear facilities. Supersedes DOE G 226.1-2

  1. Investigation of the mode of binding of a novel series ofN-benzyl...

    Office of Scientific and Technical Information (OSTI)

    of the hepatitis C viral polymerase are described herein. These compounds bind to the hepatitis C virus non-structural protein 5B (NS5B), and co-crystal structures of select...

  2. Capture and release of acid-gasses with acid-gas binding organic compounds

    DOE Patents [OSTI]

    Heldebrant, David J; Yonker, Clement R; Koech, Phillip K

    2015-03-17

    A system and method for acid-gas capture wherein organic acid-gas capture materials form hetero-atom analogs of alkyl-carbonate when contacted with an acid gas. These organic-acid gas capture materials include combinations of a weak acid and a base, or zwitterionic liquids. This invention allows for reversible acid-gas binding to these organic binding materials thus allowing for the capture and release of one or more acid gases. These acid-gas binding organic compounds can be regenerated to release the captured acid gasses and enable these organic acid-gas binding materials to be reused. This enables transport of the liquid capture compounds and the release of the acid gases from the organic liquid with significant energy savings compared to current aqueous systems.

  3. Structure of Human Toll-like Receptor 3 (TLR3) Ligand-binding...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Human Toll-like Receptor 3 (TLR3) Ligand-binding Domain Jungwoo Choe1, Matthew S. Kelker1, and Ian A. Wilson1 1Department of Molecular Biology and The Skaggs Institute for Chemical...

  4. Novel CO2 Binding Mechanism Determined via in-situ Absorption Spectroscopy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    & Theory | Center for Gas SeparationsRelevant to Clean Energy Technologies | Blandine Jerome Novel CO2 Binding Mechanism Determined via in-situ Absorption Spectroscopy & Theory

  5. Metal binding in an aluminum based metal-organic framework for carbon

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    dioxide capture | Center for Gas SeparationsRelevant to Clean Energy Technologies | Blandine Jerome Metal binding in an aluminum based metal-organic framework for carbon dioxide capture

  6. Developing Adnectins That Target SRC Co-Activator Binding to PXR: A

    Office of Scientific and Technical Information (OSTI)

    Structural Approach toward Understanding Promiscuity of PXR (Journal Article) | SciTech Connect Developing Adnectins That Target SRC Co-Activator Binding to PXR: A Structural Approach toward Understanding Promiscuity of PXR Citation Details In-Document Search Title: Developing Adnectins That Target SRC Co-Activator Binding to PXR: A Structural Approach toward Understanding Promiscuity of PXR Authors: Khan, Javed A. ; Camac, Daniel M. ; Low, Simon ; Tebben, Andrew J. ; Wensel, David L. ;

  7. Discovery of a new ATP-binding motif involved in peptidic azoline

    Office of Scientific and Technical Information (OSTI)

    biosynthesis (Journal Article) | SciTech Connect Discovery of a new ATP-binding motif involved in peptidic azoline biosynthesis Citation Details In-Document Search Title: Discovery of a new ATP-binding motif involved in peptidic azoline biosynthesis Authors: Dunbar, Kyle L. ; Chekan, Jonathan R. ; Cox, Courtney L. ; Burkhart, Brandon J. ; Nair, Satish K. ; Mitchell , Douglas A. [1] + Show Author Affiliations (UIUC) Publication Date: 2014-11-20 OSTI Identifier: 1163365 Resource Type: Journal

  8. Memo Is Homologous to Nonheme Iron Dioxygenases and Binds an ErbB2-derived

    Office of Scientific and Technical Information (OSTI)

    Phosphopeptide in Its Vestigial Active Site (Journal Article) | SciTech Connect SciTech Connect Search Results Journal Article: Memo Is Homologous to Nonheme Iron Dioxygenases and Binds an ErbB2-derived Phosphopeptide in Its Vestigial Active Site Citation Details In-Document Search Title: Memo Is Homologous to Nonheme Iron Dioxygenases and Binds an ErbB2-derived Phosphopeptide in Its Vestigial Active Site Authors: Qiu, Chen ; Lienhard, Susanne ; Hynes, Nancy E. ; Badache, Ali ; Leahy, Daniel

  9. The same pocket in menin binds both MLL and JUND but has opposite effects

    Office of Scientific and Technical Information (OSTI)

    on transcription (Journal Article) | SciTech Connect SciTech Connect Search Results Journal Article: The same pocket in menin binds both MLL and JUND but has opposite effects on transcription Citation Details In-Document Search Title: The same pocket in menin binds both MLL and JUND but has opposite effects on transcription Menin is a tumour suppressor protein whose loss or inactivation causes multiple endocrine neoplasia 1 (MEN1), a hereditary autosomal dominant tumour syndrome that is

  10. A Novel Mechanism for Binding of Galactose-terminated Glycans by the C-type

    Office of Scientific and Technical Information (OSTI)

    Carbohydrate Recognition Domain in Blood Dendritic Cell Antigen 2 (Journal Article) | SciTech Connect A Novel Mechanism for Binding of Galactose-terminated Glycans by the C-type Carbohydrate Recognition Domain in Blood Dendritic Cell Antigen 2 Citation Details In-Document Search Title: A Novel Mechanism for Binding of Galactose-terminated Glycans by the C-type Carbohydrate Recognition Domain in Blood Dendritic Cell Antigen 2 Authors: Jégouzo, Sabine A.F. ; Feinberg, Hadar ; Dungarwalla,

  11. Designing artificial metal binding peptides | Center for Bio-Inspired Solar

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Fuel Production artificial metal binding peptides 24 Oct 2012 Dong Wang is a graduate student in the Department of Chemistry and Biochemistry at Arizona State University. He is working in the lab of Professor James Allen, who is leading the Subtask 2 of the Bisfuel Center (Water oxidation catalysts). Dong's research project is focused on design and characterization of artificial peptides capable of binding divalent metals with the aim to construct an efficient water oxidation catalyst that

  12. U-039: ISC Update: BIND 9 Resolver crashes after logging an error in query.c

    Broader source: Energy.gov [DOE]

    A remote server can cause the target connected client to crash. Organizations across the Internet are reporting crashes interrupting service on BIND 9 nameservers performing recursive queries. Affected servers crash after logging an error in query.c with the following message: "INSIST(! dns_rdataset_isassociated(sigrdataset))" Multiple versions are reported as being affected, including all currently supported release versions of ISC BIND 9. ISC is actively investigating the root cause and working to produce patches which avoid the crash.

  13. U-038: BIND 9 Resolver crashes after logging an error in query.c

    Broader source: Energy.gov [DOE]

    A remote server can cause the target connected client to crash. Organizations across the Internet are reporting crashes interrupting service on BIND 9 nameservers performing recursive queries. Affected servers crash after logging an error in query.c with the following message: "INSIST(! dns_rdataset_isassociated(sigrdataset))" Multiple versions are reported as being affected, including all currently supported release versions of ISC BIND 9. ISC is actively investigating the root cause and working to produce patches which avoid the crash.

  14. Oligomycin frames a common drug-binding site in the ATP synthase

    SciTech Connect (OSTI)

    Symersky, Jindrich; Osowski, Daniel; Walters, D. Eric; Mueller, David M.

    2015-12-01

    We report the high-resolution (1.9 {angstrom}) crystal structure of oligomycin bound to the subunit c10 ring of the yeast mitochondrial ATP synthase. Oligomycin binds to the surface of the c10 ring making contact with two neighboring molecules at a position that explains the inhibitory effect on ATP synthesis. The carboxyl side chain of Glu59, which is essential for proton translocation, forms an H-bond with oligomycin via a bridging water molecule but is otherwise shielded from the aqueous environment. The remaining contacts between oligomycin and subunit c are primarily hydrophobic. The amino acid residues that form the oligomycin-binding site are 100% conserved between human and yeast but are widely different from those in bacterial homologs, thus explaining the differential sensitivity to oligomycin. Prior genetics studies suggest that the oligomycin-binding site overlaps with the binding site of other antibiotics, including those effective against Mycobacterium tuberculosis, and thereby frames a common 'drug-binding site.' We anticipate that this drug-binding site will serve as an effective target for new antibiotics developed by rational design.

  15. Elevated epidermal growth factor receptor binding in plutonium-induced lung tumors from dogs

    SciTech Connect (OSTI)

    Leung, F.C.; Bohn, L.R.; Dagle, G.E. )

    1991-04-01

    The objective of this study is to examine and characterize epidermal growth factor receptor (EGF-R) binding in inhaled plutonium-induced canine lung-tumor tissue and to compare it with that in normal canine lung tissue. Crude membrane preparations from normal and lung-tumor tissue from beagle dogs were examined in a radioreceptor assay, using {sup 125}I-labeled epidermal growth factor (EGF) as a ligand. Specific EGF receptor binding was determined in the presence of excess unlabeled EGF. We have examined EGF receptor binding in eight lung-tumor samples obtained from six dogs. Epidermal growth factor receptor binding was significantly greater in lung-tumor samples (31.38%) compared with that in normal lung tissue (3.76%). Scatchard plot analysis from the displacement assay revealed that there was no statistical difference in the binding affinity but significantly higher concentration of EGF-R sites in the lung-tumor tissue (619 fmol/mg) than in normal lung tissue (53 fmol/mg). The increase in EGF-R number in plutonium-induced dog lung tumors does not seem to correlate with increase in the initial lung burden exposure to plutonium. Our results demonstrate that there is a significant increase in EGF-R binding in inhaled plutonium-induced dog lung tumors.

  16. In silico identification of anthropogenic chemicals as ligands of zebrafish sex hormone binding globulin

    SciTech Connect (OSTI)

    Thorsteinson, Nels; Ban, Fuqiang; Santos-Filho, Osvaldo; Tabaei, Seyed M.H. [Prostate Centre at the Vancouver General Hospital, University of British Columbia, 2660 Oak Street, Vancouver, BC, V6H 3Z6 (Canada); Miguel-Queralt, Solange; Underhill, Caroline [Department of Obstetrics and Gynecology, University of British Columbia, Child and Family Research Institute, 950 West 28th Avenue, Vancouver, BC, V5Z 4H4 (Canada); Cherkasov, Artem [Prostate Centre at the Vancouver General Hospital, University of British Columbia, 2660 Oak Street, Vancouver, BC, V6H 3Z6 (Canada)], E-mail: artc@interchange.ubc.ca; Hammond, Geoffrey L. [Department of Obstetrics and Gynecology, University of British Columbia, Child and Family Research Institute, 950 West 28th Avenue, Vancouver, BC, V5Z 4H4 (Canada)

    2009-01-01

    Anthropogenic compounds with the capacity to interact with the steroid-binding site of sex hormone binding globulin (SHBG) pose health risks to humans and other vertebrates including fish. Building on studies of human SHBG, we have applied in silico drug discovery methods to identify potential binders for SHBG in zebrafish (Danio rerio) as a model aquatic organism. Computational methods, including; homology modeling, molecular dynamics simulations, virtual screening, and 3D QSAR analysis, successfully identified 6 non-steroidal substances from the ZINC chemical database that bind to zebrafish SHBG (zfSHBG) with low-micromolar to nanomolar affinities, as determined by a competitive ligand-binding assay. We also screened 80,000 commercial substances listed by the European Chemicals Bureau and Environment Canada, and 6 non-steroidal hits from this in silico screen were tested experimentally for zfSHBG binding. All 6 of these compounds displaced the [{sup 3}H]5{alpha}-dihydrotestosterone used as labeled ligand in the zfSHBG screening assay when tested at a 33 {mu}M concentration, and 3 of them (hexestrol, 4-tert-octylcatechol, and dihydrobenzo(a)pyren-7(8H)-one) bind to zfSHBG in the micromolar range. The study demonstrates the feasibility of large-scale in silico screening of anthropogenic compounds that may disrupt or highjack functionally important protein:ligand interactions. Such studies could increase the awareness of hazards posed by existing commercial chemicals at relatively low cost.

  17. Principles of ground relaying for high voltage and extra high voltage transmission lines

    SciTech Connect (OSTI)

    Griffin, C.H.

    1983-02-01

    This paper is a tutorial discussion of the basic principles of ground relaying for high voltage and extra high voltage transmission lines. Three different HV configurations are considered: Long lines, lines with a weak mid-point station, and mutually-coupled lines. Application criteria for EHV circuits are also discussed, and specific setting calculations are included where appropriate.

  18. Angular output of hollow, metal-lined, waveguide Raman sensors

    SciTech Connect (OSTI)

    Biedrzycki, Stephen; Buric, Michael P.; Falk, Joel; Woodruff, Steven D.

    2012-04-20

    Hollow, metal-lined waveguides used as gas sensors based on spontaneous Raman scattering are capable of large angular collection. The collection of light from a large solid angle implies the collection of a large number of waveguide modes. An accurate estimation of the propagation losses for these modes is required to predict the total collected Raman power. We report a theory/experimental comparison of the Raman power collected as a function of the solid angle and waveguide length. New theoretical observations are compared with previous theory appropriate only for low-order modes. A cutback experiment is demonstrated to verify the validity of either theory. The angular distribution of Raman light is measured using aluminum and silver-lined waveguides of varying lengths.

  19. Plasmids and packaging cell lines for use in phage display

    DOE Patents [OSTI]

    Bradbury, Andrew M.

    2012-07-24

    The invention relates to a novel phagemid display system for packaging phagemid DNA into phagemid particles which completely avoids the use of helper phage. The system of the invention incorporates the use of bacterial packaging cell lines which have been transformed with helper plasmids containing all required phage proteins but not the packaging signals. The absence of packaging signals in these helper plasmids prevents their DNA from being packaged in the bacterial cell, which provides a number of significant advantages over the use of both standard and modified helper phage. Packaged phagemids expressing a protein or peptide of interest, in fusion with a phage coat protein such as g3p, are generated simply by transfecting phagemid into the packaging cell line.

  20. Undersea line planned to transmit to an island

    SciTech Connect (OSTI)

    Not Available

    1993-08-09

    The electric utility serving Nantucket Island in Massachusetts, which until now has generated its own power, plans to lay 25 miles of transmission cable to connect with New England's mainland grid. The line will allow the utility to purchase less costly power and retire several old generators, improving both reliability and air quality on the island. Nantucket Electric Co. says the 33-Mw submarine link, costing at least $23 million, probably will connect with a line near the elbow on Cape Cod. The undersea cable will be as deep as 60 ft. Nantucket Electric plans to form a partnership within a few months with a mainland utility or private producer that would help finance the project and sell the power. The island utility has preliminary approval by the state Industrial Finance Agency for a tax-exempt bond issue to finance the cable, contingent on its finding a partner.

  1. Strengthening Line Management Oversight and Federal Monitoring of Nuclear Facilities

    Office of Environmental Management (EM)

    Strengthening Line Management Oversight and Federal Monitoring of Nuclear Facilities Standard Review Plan Volume 4 - Nuclear Safety Basis Program Review During Facility Decommissioning and Environmental Restoration February 2015 i Standard Review Plan Volume 4 Nuclear Safety Basis Program Review during Facility Decommissioning and Environmental Restoration Facility Life Cycle Applicability CD-1 CD-2 CD-3 CD-4 Operations and Transitions Decommissioning & Environmental Restoration February

  2. Clean Line Plains and Eastern Section 1222 Decision Documents

    Broader source: Energy.gov [DOE]

    On March 25, 2016, U.S. Secretary of Energy Dr. Ernest Moniz announced that the Department of Energy (DOE) will participate in the development of the Plains & Eastern Clean Line project (Project), a major clean energy infrastructure project. Through section 1222 of the Energy Policy Act of 2005, Congress authorized DOE to promote electric transmission development. DOE is using this authority to participate in the development of the Project.

  3. Gas insulated transmission line with insulators having field controlling recesses

    DOE Patents [OSTI]

    Cookson, Alan H. (Pittsburgh, PA); Pederson, Bjorn O. (Chelmsford, MA)

    1984-01-01

    A gas insulated transmission line having a novel insulator for supporting an inner conductor concentrically within an outer sheath. The insulator has a recess contiguous with the periphery of one of the outer and inner conductors. The recess is disposed to a depth equal to an optimum gap for the dielectric insulating fluid used for the high voltage insulation or alternately disposed to a large depth so as to reduce the field at the critical conductor/insulator interface.

  4. A reactor core on-line monitoring program - COMP

    SciTech Connect (OSTI)

    Wang, C.; Wu, H.; Cao, L.

    2012-07-01

    A program named COMP is developed for on-line monitoring PWRs' in-core power distribution in this paper. Harmonics expansion method is used in COMP. The Unit 1 reactor of Daya Bay Nuclear Power Plant (Daya Bay NPP) in China is considered for verification. The numerical results show that the maximum relative error between measurement and reconstruction results from COMP is less than 5%, and the computing time is short, indicating that COMP is capable for online monitoring PWRs. (authors)

  5. Retractable pin dual in-line package test clip

    DOE Patents [OSTI]

    Bandzuch, Gregory S.; Kosslow, William J.

    1996-01-01

    This invention is a Dual In-Line Package (DIP) test clip for use when troubleshooting circuits containing DIP integrated circuits. This test clip is a significant improvement over existing DIP test clips in that it has retractable pins which will permit troubleshooting without risk of accidentally shorting adjacent pins together when moving probes to different pins on energized circuits or when the probe is accidentally bumped while taking measurements.

  6. Calcium Hexa Aluminate Linings for Alkaline Environments - Energy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Innovation Portal Biomass and Biofuels Biomass and Biofuels Find More Like This Return to Search Calcium Hexa Aluminate Linings for Alkaline Environments Oak Ridge National Laboratory Contact ORNL About This Technology Technology Marketing SummaryA new composition of calcium hexa aluminate developed at ORNL is a highly effective chemical barrier against corrosive materials inside storage, process, and transport vessel containers. The CA6 composition protects containers from the critical

  7. Wireless Sensor Network for Electric Transmission Line Monitoring

    Office of Scientific and Technical Information (OSTI)

    Wireless Sensor Network for Electric Transmission Line Monitoring Final Technical Report DOE Award: DE-FC26-06NT42795 January 2010 Revised March 2010 University of Louisville, Genscape Inc. Principle Investigator: Bruce Alphenaar PhD George Lin PhD, Bill Brown BE, Deirdre Alphenaar PhD, Walter Jones PhD, Chris Pettus ME, Yang Xu PhD, Devin Phinney BS 2 Contents Executive Summary

  8. Improved Lining Material for Gasifiers Could Lead to Wider

    Broader source: Energy.gov (indexed) [DOE]

    Commercialization of Clean and Efficient Energy Technology | Department of Energy Refractories removed from adjacent positions in a slagging gasifier. The NETL refractory (right) has approximately 50 percent more material remaining after the test. Refractories removed from adjacent positions in a slagging gasifier. The NETL refractory (right) has approximately 50 percent more material remaining after the test. Washington, DC - A new improved-performance refractory lining material developed

  9. DOE - NNSA/NSO -- SiteLines Web Edition

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Briefs NTS Public Tours Factsheets Speakers Bureau To Your Health Acronyms Mailing List Information Request Publishing Information Questions/Contact Masthead Archives FAQs/QUESTIONS SUBJECT INDEX SITEMAP SEARCH CONTACT US ABOUT US HELP ACRONYMS FOIA PRIVACY ACT WEBSITE POLICIES HOMEPAGE Home > Library> SiteLines U.S. DOE/NNSA - Nevada Site Office May/June 2008 - Issue 131 A publication for all members of the NNSA/NSO family Criticality Experiments Facility Construction Passes Halfway

  10. Jefferson Lab announces Fall Science Series line up | Jefferson Lab

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    announces Fall Science Series line up September 5, 2003 Jefferson Lab hosts Fall and Spring Science Series to engage the public in a range of current scientific topics. Guest speakers from across the nation are invited to the Lab to present their sometimes entertaining or thought-provoking, but always informative lectures or demonstrations. The Jefferson Lab Fall Science Series kicks off on Tuesday, October 7, with Michael Henchman, a chemistry professor from Brandeis University, Massachusetts.

  11. second line of defense | National Nuclear Security Administration

    National Nuclear Security Administration (NNSA)

    second line of defense | National Nuclear Security Administration Facebook Twitter Youtube Flickr RSS People Mission Managing the Stockpile Preventing Proliferation Powering the Nuclear Navy Emergency Response Recapitalizing Our Infrastructure Countering Nuclear Terrorism About Our Programs Our History Who We Are Our Leadership Our Locations Budget Our Operations Library Bios Congressional Testimony Fact Sheets Newsletters Press Releases Photo Gallery Jobs Apply for Our Jobs Our Jobs Working at

  12. AMS Ground Truth Measurements: Calibration and Test Lines

    SciTech Connect (OSTI)

    Wasiolek, P.

    2013-11-01

    Airborne gamma spectrometry is one of the primary techniques used to define the extent of ground contamination after a radiological incident. Its usefulness was demonstrated extensively during the response to the Fukushima nuclear power plant (NPP) accident in March-May 2011. To map ground contamination a set of scintillation detectors is mounted on an airborne platform (airplane or helicopter) and flown over contaminated areas. The acquisition system collects spectral information together with the aircraft position and altitude every second. To provide useful information to decision makers, the count rate data expressed in counts per second (cps) needs to be converted to the terrestrial component of the exposure rate 1 m above ground, or surface activity of isotopes of concern. This is done using conversion coefficients derived from calibration flights. During a large scale radiological event, multiple flights may be necessary and may require use of assets from different agencies. However, as the production of a single, consistent map product depicting the ground contamination is the primary goal, it is critical to establish very early into the event a common calibration line. Such a line should be flown periodically in order to normalize data collected from different aerial acquisition systems and potentially flown at different flight altitudes and speeds. In order to verify and validate individual aerial systems, the calibration line needs to be characterized in terms of ground truth measurements. This is especially important if the contamination is due to short-lived radionuclides. The process of establishing such a line, as well as necessary ground truth measurements, is described in this document.

  13. Monitoring pipe line stress due to ground displacement

    SciTech Connect (OSTI)

    Greenwood, J.H. Jr.

    1986-04-01

    Northwest Pipeline Corp. has a large-diameter natural gas pipe line system from Ignacio, Colo., to Sumas, Wash. At Douglas Pass in Colorado, large landslides required several sections of the line to be relocated outside the slide areas: 4,400 ft of new line in April 1962 and 3,200 ft in March 1963. No serious disruptions occurred for the next 16 years. Then in July 1979, some 1,200 ft had to be relocated. From 1980 to date, many landslides in the Douglas Pass area have caused new deformations, with the springs of 1983 and 1984 being the worst years. In 1980, Northwest Pipeline began engineering and geotechnical studies of the landslide problems. These led to instrumentation and pipe monitoring which indicated that pipe failure can be predicted and prevented if important slope deformations or increases in pipe stresses are detected early enough to implement some mitigating measures. Excavation of the pipe to relieve the stresses was used in most cases. The method was so successful that no pipe failure occurred in 1984 within instrumented sections, in spite of the exceptionally bad climatic conditions experienced.

  14. Boron-Lined Multichamber and Conventional Neutron Proportional Counter Tests

    SciTech Connect (OSTI)

    Woodring, Mitchell L.; Ely, James H.; Kouzes, Richard T.; Stromswold, David C.

    2010-09-07

    Radiation portal monitors used for interdiction of illicit materials at borders include highly sensitive neutron detection systems. The main reason for having neutron detection capability is to detect fission neutrons from plutonium. The currently deployed radiation portal monitors (RPMs) from Ludlum and Science Applications International Corporation (SAIC) use neutron detectors based upon 3He-filled gas proportional counters, which are the most common large neutron detector. There is a declining supply of 3He in the world, and thus, methods to reduce the use of this gas in RPMs with minimal changes to the current system designs and sensitivity to cargo-borne neutrons are being investigated. Four technologies have been identified as being currently commercially available, potential alternative neutron detectors to replace the use of 3He in RPMs. These technologies are: 1) Boron trifluoride (BF3)-filled proportional counters, 2) Boron-lined proportional counters, 3) Lithium-loaded glass fibers, and 4) Coated non-scintillating plastic fibers. In addition, a few other companies have detector technologies that might be competitive in the near term as an alternative technology. Reported here are the results of tests of a boron-lined, multichamber proportional counter manufactured by LND, Inc. Also reported are results obtained with an earlier design of conventional, boron-lined, proportional counters from LND. This testing measured the required performance for neutron detection efficiency and gamma-ray rejection capabilities of the detectors.

  15. Discovery of a transiting planet near the snow-line

    SciTech Connect (OSTI)

    Kipping, D. M.; Torres, G.; Buchhave, L. A.; Kenyon, S. J. [Harvard-Smithsonian Center for Astrophysics, Cambridge, MA 02138 (United States); Henze, C.; Bryson, S. T. [NASA Ames Research Center, Moffett Field, CA 94035 (United States); Isaacson, H.; Kolbl, R.; Marcy, G. W. [University of California, Berkeley, CA 94720 (United States); Stassun, K. [Department of Physics and Astronomy, Vanderbilt University, 1807 Station B, Nashville, TN 37235 (United States); Bastien, F., E-mail: dkipping@cfa.harvard.edu [Physics Department, Fisk University, 1000 17th Ave. N, Nashville, TN 37208 (United States)

    2014-11-01

    In most theories of planet formation, the snow-line represents a boundary between the emergence of the interior rocky planets and the exterior ice giants. The wide separation of the snow-line makes the discovery of transiting worlds challenging, yet transits would allow for detailed subsequent characterization. We present the discovery of Kepler-421b, a Uranus-sized exoplanet transiting a G9/K0 dwarf once every 704.2 days in a near-circular orbit. Using public Kepler photometry, we demonstrate that the two observed transits can be uniquely attributed to the 704.2 day period. Detailed light curve analysis with BLENDER validates the planetary nature of Kepler-421b to >4? confidence. Kepler-421b receives the same insolation as a body at ?2 AU in the solar system, as well as a Uranian albedo, which would have an effective temperature of ?180 K. Using a time-dependent model for the protoplanetary disk, we estimate that Kepler-421b's present semi-major axis was beyond the snow-line after ?3 Myr, indicating that Kepler-421b may have formed at its observed location.

  16. Review of trigger and on-line processors at SLAC

    SciTech Connect (OSTI)

    Lankford, A.J.

    1984-07-01

    The role of trigger and on-line processors in reducing data rates to manageable proportions in e/sup +/e/sup -/ physics experiments is defined not by high physics or background rates, but by the large event sizes of the general-purpose detectors employed. The rate of e/sup +/e/sup -/ annihilation is low, and backgrounds are not high; yet the number of physics processes which can be studied is vast and varied. This paper begins by briefly describing the role of trigger processors in the e/sup +/e/sup -/ context. The usual flow of the trigger decision process is illustrated with selected examples of SLAC trigger processing. The features are mentioned of triggering at the SLC and the trigger processing plans of the two SLC detectors: The Mark II and the SLD. The most common on-line processors at SLAC, the BADC, the SLAC Scanner Processor, the SLAC FASTBUS Controller, and the VAX CAMAC Channel, are discussed. Uses of the 168/E, 3081/E, and FASTBUS VAX processors are mentioned. The manner in which these processors are interfaced and the function they serve on line is described. Finally, the accelerator control system for the SLC is outlined. This paper is a survey in nature, and hence, relies heavily upon references to previous publications for detailed description of work mentioned here. 27 references, 9 figures, 1 table.

  17. Unraveling a Hotspot for TCR Recognition on HLA-A2: Evidence Against the Existence of Peptide-independent TCR Binding Determinants

    SciTech Connect (OSTI)

    Gagnon, Susan J.; Borbulevych, Oleg Y.; Davis-Harrison, Rebecca L.; Baxter, Tiffany K.; Clemens, John R.; Armstrong, Kathryn M.; Turner, Richard V.; Damirjian, Marale; Biddison, William E.; Baker, Brian M.

    2010-07-19

    T cell receptor (TCR) recognition of peptide takes place in the context of the major histocompatibility complex (MHC) molecule, which accounts for approximately two-thirds of the peptide/MHC buried surface. Using the class I MHC HLA-A2 and a large panel of mutants, we have previously shown that surface mutations that disrupt TCR recognition vary with the identity of the peptide. The single exception is Lys66 on the HLA-A2 {alpha}1 helix, which when mutated to alanine disrupts recognition for 93% of over 250 different T cell clones or lines, independent of which peptide is bound. Thus, Lys66 could serve as a peptide-independent TCR binding determinant. Here, we have examined the role of Lys66 in TCR recognition of HLA-A2 in detail. The structure of a peptide/HLA-A2 molecule with the K66A mutation indicates that although the mutation induces no major structural changes, it results in the exposure of a negatively charged glutamate (Glu63) underneath Lys66. Concurrent replacement of Glu63 with glutamine restores TCR binding and function for T cells specific for five different peptides presented by HLA-A2. Thus, the positive charge on Lys66 does not serve to guide all TCRs onto the HLA-A2 molecule in a manner required for productive signaling. Furthermore, electrostatic calculations indicate that Lys66 does not contribute to the stability of two TCR-peptide/HLA-A2 complexes. Our findings are consistent with the notion that each TCR arrives at a unique solution of how to bind a peptide/MHC, most strongly influenced by the chemical and structural features of the bound peptide. This would not rule out an intrinsic affinity of TCRs for MHC molecules achieved through multiple weak interactions, but for HLA-A2 the collective mutational data place limits on the role of any single MHC amino acid side-chain in driving TCR binding in a peptide-independent fashion.

  18. Binding of undamaged double stranded DNA to vaccinia virus uracil-DNA glycosylase

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Schormann, Norbert; Banerjee, Surajit; Ricciardi, Robert; Chattopadhyay, Debasish

    2015-06-02

    Background: Uracil-DNA glycosylases are evolutionarily conserved DNA repair enzymes. However, vaccinia virus uracil-DNA glycosylase (known as D4), also serves as an intrinsic and essential component of the processive DNA polymerase complex during DNA replication. In this complex D4 binds to a unique poxvirus specific protein A20 which tethers it to the DNA polymerase. At the replication fork the DNA scanning and repair function of D4 is coupled with DNA replication. So far, DNA-binding to D4 has not been structurally characterized. Results: This manuscript describes the first structure of a DNA-complex of a uracil-DNA glycosylase from the poxvirus family. This alsomore » represents the first structure of a uracil DNA glycosylase in complex with an undamaged DNA. In the asymmetric unit two D4 subunits bind simultaneously to complementary strands of the DNA double helix. Each D4 subunit interacts mainly with the central region of one strand. DNA binds to the opposite side of the A20-binding surface on D4. In comparison of the present structure with the structure of uracil-containing DNA-bound human uracil-DNA glycosylase suggests that for DNA binding and uracil removal D4 employs a unique set of residues and motifs that are highly conserved within the poxvirus family but different in other organisms. Conclusion: The first structure of D4 bound to a truly non-specific undamaged double-stranded DNA suggests that initial binding of DNA may involve multiple non-specific interactions between the protein and the phosphate backbone.« less

  19. Improvement of the mechanical reliability of monolithic refractory linings for coal gasification process vessels. Final report

    SciTech Connect (OSTI)

    Potter, R.A.

    1981-09-01

    Eighteen heat-up tests were run on nine standard and experimental dual component monolithic refractory concrete linings. These tests were run with a five foot diameter by 14-ft high Pressure Vessel/Test Furnace designed to accommodate a 12-inch thick by 5-ft high refractory lining, heat the hot face to 2000/sup 0/F and expose the lining to air or steam pressures up to 150 psig. Results obtained from standard type linings in the test facility indicated that lining degradation duplicated that observed in field installations. The lining performance was significantly improved due to information gained from a systematic study of the cracking that occurred in the linings; the analysis of the lining strains, shell stresses and acoustic emission results; and the stress analyses performed on the standard and experimental lining designs with the finite element analysis computer programs, REFSAM and RESGAP.

  20. Modulation of FadR Binding Capacity for Acyl-CoA Fatty Acids Through Structure-Guided Mutagenesis

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Bacik, John-Paul; Yeager, Chris M.; Twary, Scott N.; Martí-Arbona, Ricardo

    2015-09-18

    FadR is a versatile global regulator in Escherichia coli that controls fatty acid metabolism and thereby modulates the ability of this bacterium to grow using fatty acids or acetate as the sole carbon source. FadR regulates fatty acid metabolism in response to intra-cellular concentrations of acyl-CoA lipids. The ability of FadR to bind acyl-CoA fatty acids is hence of significant interest for the engineering of biosynthetic pathways for the production of lipid-based biofuels and commodity chemicals. Based on the available crystal structure of E. coli bound to myristoyl- CoA, we predicted amino acid positions within the effector binding pocket thatmore » would alter the ability of FadR to bind acyl-CoA fatty acids without affecting DNA binding. We utilized fluorescence polarization to characterize the in-vitro binding properties of wild type and mutant FadR. We found that a Leu102Ala mutant enhanced binding of the effector, likely by increasing the size of the binding pocket for the acyl moiety of the molecule. Conversely, the elimination of the guanidine side chain (Arg213Ala and Arg213Met mutants) of the CoA moiety binding site severely diminished the ability of FadR to bind the acyl-CoA effector. These results demonstrate the ability to fine tune FadR binding capacity. The validation of an efficient method to fully characterize all the binding events involved in the specific activity (effector and DNA operator binding) of FadR has allowed us to increase our understanding of the role of specific amino acids in the binding and recognition of acyl-CoA fatty acids and will greatly facilitate efforts aimed at engineering tunable FadR regulators for synthetic biology.« less

  1. Beyond the detergent effect: a binding site for sodium dodecyl sulfate (SDS) in mammalian apoferritin

    SciTech Connect (OSTI)

    Liu, Renyu Bu, Weiming; Xi, Jin; Mortazavi, Shirin R.; Cheung-Lau, Jasmina C.; Dmochowski, Ivan J.; Loll, Patrick J.

    2012-05-01

    Using X-ray crystallography and isothermal titration calorimetry, we show that sodium dodecyl sulfate (SDS) binds specifically to a pre-formed internal cavity in horse-spleen apoferritin. Although sodium dodecyl sulfate (SDS) is widely used as an anionic detergent, it can also exert specific pharmacological effects that are independent of the surfactant properties of the molecule. However, structural details of how proteins recognize SDS are scarce. Here, it is demonstrated that SDS binds specifically to a naturally occurring four-helix bundle protein: horse apoferritin. The X-ray crystal structure of the apoferritin–SDS complex was determined at a resolution of 1.9 Å and revealed that the SDS binds in an internal cavity that has previously been shown to recognize various general anesthetics. A dissociation constant of 24 ± 9 µM at 293 K was determined by isothermal titration calorimetry. SDS binds in this cavity by bending its alkyl tail into a horseshoe shape; the charged SDS head group lies in the opening of the cavity at the protein surface. This crystal structure provides insights into the protein–SDS interactions that give rise to binding and may prove useful in the design of novel SDS-like ligands for some proteins.

  2. Nucleotide-binding flexibility in ultrahigh-resolution structures of the SRP GTPase Ffh

    SciTech Connect (OSTI)

    Ramirez, Ursula D.; Focia, Pamela J.; Freymann, Douglas M.

    2008-10-01

    Crystal structures of the Ffh NG GTPase domain at < 1.24 Å resolution reveal multiple overlapping nucleotide binding modes. Two structures of the nucleotide-bound NG domain of Ffh, the GTPase subunit of the bacterial signal recognition particle (SRP), have been determined at ultrahigh resolution in similar crystal forms. One is GDP-bound and one is GMPPCP-bound. The asymmetric unit of each structure contains two protein monomers, each of which exhibits differences in nucleotide-binding conformation and occupancy. The GDP-bound Ffh NG exhibits two binding conformations in one monomer but not the other and the GMPPCP-bound protein exhibits full occupancy of the nucleotide in one monomer but only partial occupancy in the other. Thus, under the same solution conditions, each crystal reveals multiple binding states that suggest that even when nucleotide is bound its position in the Ffh NG active site is dynamic. Some differences in the positioning of the bound nucleotide may arise from differences in the crystal-packing environment and specific factors that have been identified include the relative positions of the N and G domains, small conformational changes in the P-loop, the positions of waters buried within the active site and shifts in the closing loop that packs against the guanine base. However, ‘loose’ binding may have biological significance in promoting facile nucleotide exchange and providing a mechanism for priming the SRP GTPase prior to its activation in its complex with the SRP receptor.

  3. Protein binding studies of technetium-99m-labeled phosphine and isocyanide cationic complexes

    SciTech Connect (OSTI)

    Zanelli, G.D.; Cook, N.; Lahiri, A.; Ellison, D.; Webbon, P.; Woolley, G.

    1988-01-01

    Most /sup 99m/Tc/phosphine/isocyanide complexes synthesized to date show preferential uptake by the myocardium of many animal species but not in man. A new complex has been synthesized, (/sup 99m/Tc(DEPE)2(CNR)2), +(DEPIC), where R = t - butyl isocyanide, which in three animal species images the myocardium very well, but in humans it remains primarily in the blood pool. One reason for the difference in the behavior of these complexes in different species could be the characteristics of their binding to plasma proteins. The protein binding characteristics of DEPIC and two other well-known complexes have therefore been studied. Whereas the other complexes bind nonspecifically to many proteins both in animal and human plasma, DEPIC binds almost exclusively to prealbumin in humans but nonspecifically to other proteins in the rabbit. The binding sites in human plasma appear to be those normally occupied by thyroxine on the prealbumin tetramer and these can be blocked by sodium salicylate.

  4. On-line gas chromatographic analysis of airborne particles

    DOE Patents [OSTI]

    Hering, Susanne V. (Berkeley, CA); Goldstein, Allen H. (Orinda, CA)

    2012-01-03

    A method and apparatus for the in-situ, chemical analysis of an aerosol. The method may include the steps of: collecting an aerosol; thermally desorbing the aerosol into a carrier gas to provide desorbed aerosol material; transporting the desorbed aerosol material onto the head of a gas chromatography column; analyzing the aerosol material using a gas chromatograph, and quantizing the aerosol material as it evolves from the gas chromatography column. The apparatus includes a collection and thermal desorption cell, a gas chromatograph including a gas chromatography column, heated transport lines coupling the cell and the column; and a quantization detector for aerosol material evolving from the gas chromatography column.

  5. Field quality measurements of a 2-Tesla transmission line magnet

    SciTech Connect (OSTI)

    Velev, G.V.; Foster, W.; Kashikhin, V.; Mazur, P.; Oleck, A.; Piekarz, H.; Schlabach, P.; Sylvester, C.; Wake, M.; /KEK, Tsukuba

    2005-09-01

    A prototype 2-Tesla superconducting transmission line magnet for future hadron colliders was designed, built and tested at Fermilab. The 1.5 m long, combined-function gradient-dipole magnet has a vertical pole aperture of 20 mm. To measure the magnetic field quality in such a small magnet aperture, a specialized rotating coil of 15.2 mm diameter, 0.69 m long was fabricated. Using this probe, a program of magnetic field quality measurements was successfully performed. Results of the measurements are presented and discussed.

  6. Note: Characteristic beam parameter for the line electron gun

    SciTech Connect (OSTI)

    Iqbal, M.; Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049 ; Islam, G. U.; Zhou, Z.; Chi, Y.

    2013-11-15

    We have optimized the beam parameters of line source electron gun using Stanford Linear Accelerator Centre electron beam trajectory program (EGUN), utilizing electrostatic focusing only. We measured minimum beam diameter as 0.5 mm that corresponds to power density of 68.9 kW/cm{sup 2} at 13.5 mm in the post-anode region which is more than two-fold (33 kW/cm{sup 2}), of the previously reported results. The gun was operated for the validation of the theoretical results and found in good agreement. The gun is now without any magnetic and electrostatic focusing thus much simpler and more powerful.

  7. The performance of the MICE muon beam line

    SciTech Connect (OSTI)

    Rayner, Mark Alastair

    2011-10-06

    The Muon Ionization Cooling Experiment is one lattice cell of a cooling channel suitable for conditioning the muon beam at the front end of a Neutrino Factory or Muon Collider. The beam line designed to transport muons into MICE has been installed, and data was collected in 2010. In this paper the method of reconstructing longitudinal momentum and transverse trace space using two timing detectors is discussed, and a preliminary simulation of the performance of a measured beam in the cooling channel is presented.

  8. DOE - NNSA/NSO -- SiteLines - Issue 144

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    SITEMAP SEARCH CONTACT US ABOUT US HELP ACRONYMS FOIA PRIVACY ACT WEBSITE POLICIES HOMEPAGE Home > Library > SiteLines U.S. DOE/NNSA - Nevada Site Office July/August 2010 - Issue 144 A publication for all members of the NNSA/NSO family RNCTEC One Year Later: Mission Growth and Expansion It was one year ago that the National Nuclear Security Administration/ Nevada Site Office (NNSA/NSO) completed the start-up of the Radiological/Nuclear Countermeasures Test and Evaluation Complex (RNCTEC)

  9. DOE - NNSA/NSO -- SiteLines Web Edition

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Face to Face Calendar NTS Public Tours Factsheets Speakers Bureau Acronyms Mailing List Information Request Publishing Information Questions/Contact Credits Archives FAQs/QUESTIONS SUBJECT INDEX SITEMAP SEARCH CONTACT US ABOUT US HELP ACRONYMS FOIA PRIVACY ACT WEBSITE POLICIES HOMEPAGE Home > Library > SiteLines U.S. DOE/NNSA - Nevada Site Office April/May 2007 - Issue 125 A publication for all members of the NNSA/NSO family Small Thermos experiments yield great data on aging stockpile The

  10. DOE - NNSA/NSO -- SiteLines Web Edition

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Environmental Management Face-to-Face Calendar NTS Public Tours Factsheets Speakers Bureau To Your Health Acronyms Mailing List Information Request Publishing Information Questions/Contact Masthead Archives FAQs/QUESTIONS SUBJECT INDEX SITEMAP SEARCH CONTACT US ABOUT US HELP ACRONYMS FOIA PRIVACY ACT WEBSITE POLICIES HOMEPAGE Home > Library > SiteLines U.S. DOE/NNSA - Nevada Site Office August/September 2007 - Issue 127 A publication for all members of the NNSA/NSO family New Test Site is

  11. DOE - NNSA/NSO -- SiteLines Web Edition

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Beyond the Call Face-to-Face Calendar NTS Public Tours Factsheets Speakers Bureau To Your Health Acronyms Mailing List Information Request Publishing Information Questions/Contact Masthead Archives FAQs/QUESTIONS SUBJECT INDEX SITEMAP SEARCH CONTACT US ABOUT US HELP ACRONYMS FOIA PRIVACY ACT WEBSITE POLICIES HOMEPAGE Home > Library > SiteLines U.S. DOE/NNSA - Nevada Site Office October/November 2007 - Issue 128 A publication for all members of the NNSA/NSO family CEF conducts three primary

  12. DOE - NNSA/NSO -- SiteLines Web Edition

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Face-to-Face NTS Public Tours Factsheets Speakers Bureau Acronyms Mailing List Information Request Publishing Information Questions/Contact Masthead Archives FAQs/QUESTIONS SUBJECT INDEX SITEMAP SEARCH CONTACT US ABOUT US HELP ACRONYMS FOIA PRIVACY ACT WEBSITE POLICIES HOMEPAGE Home > Library > SiteLines U.S. DOE/NNSA - Nevada Site Office March/April 2008 - Issue 130 A publication for all members of the NNSA/NSO family New addition to NSO Teri Holland has joined NSO as the new Assistant

  13. Microsoft Word - notification of sampling line loss.doc

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    68:UFC5486 Department of Energy Carlsbad Field Office P. O. Box 3090 Carlsbad, New Mexico 88221 July 17, 2008 Mr. James Bearzi, Chief Hazardous Waste Bureau New Mexico Environment Department 2905 Rodeo Park Drive East, Building 1 Santa Fe, NM 87505-6303 Subject: Notification of Sampling Line Loss, Waste Isolation Pilot Plant EPA I.D. Number NM4890139088 Dear Mr. Bearzi: As required under Permit Condition IV.F.5.e, the Permittees are hereby notifying the New Mexico Environment Department (NMED)

  14. Electro Acceleration in a Geomagnetic Field Line Resonance

    SciTech Connect (OSTI)

    Peter Damiano and J.R. Johnson

    2012-08-17

    A hybrid MHD kinetic electron model in dipolar coordinates is used to sim- ulate the upward current region of a geomagnetic Field Line Resonance (FLR) system for a realistic ambient electron temperatures of a keV. It is found that mirror force e ects result in potential drops su#14;cient to accelerate electrons to energies in excess of a keV in support of eld aligned currents on the or- der of 0.5 #22;µA/m2. The wave energy dissipated in this acceleration would com- pletely damp an undriven FLR with an equatorial width of 0.5 RE within two resonance cycles.

  15. Southline Transmission Line Project - Volume 1 Front Matter

    Energy Savers [EERE]

    WESTERN BLM/NM/PL-14-01-1610 * DOE/EIS-0474 Southline Transmission Line Project Final Environmental Impact Statement Volume 1 of 4 October 2015 MISSION STATEMENT The Bureau of Land Management is responsible for stewardship of our public lands. The BLM is committed to manage, protect, and improve these lands in a manner to serve the needs of the American people. Management is based upon the principles of multiple use and sustained yield of our Nation's resources within the framework of

  16. Barriers in the transition to global chaos in collisionless magnetic reconnection. II. Field line spectroscopy

    SciTech Connect (OSTI)

    Borgogno, D.; Grasso, D.; Pegoraro, F.; Schep, T. J.

    2011-10-15

    The transitional phase from local to global chaos in the magnetic field of a reconnecting current layer is investigated. The identification of the ridges in the field of the finite time Lyapunov exponent as barriers to the field line motion is carried out adopting the technique of field line spectroscopy to analyze the radial position of a field line while it winds its way through partial stochastic layers and to compare the frequencies of the field line motion with the corresponding frequencies of the distinguished hyperbolic field lines that are the nonlinear generalizations of linear X-lines.

  17. EA-352 NaturEner Tie Line, LLC | Department of Energy

    Energy Savers [EERE]

    2 NaturEner Tie Line, LLC EA-352 NaturEner Tie Line, LLC Order authorizing NaturEner Tie Line, LLC to export electric energy to Canada PDF icon EA-352 NaturEner Tie Line, LLC More Documents & Publications Application to Export Electric Energy OE Docket No. EA-352 NaturEner Tie Line, LLC Application to Export Electric Energy OE Docket No. EA-352 NaturEner Tie Line, LLC: Federal Register Notice Volume 74, No. 98 - May 22, 2009 EA-344 Twin Cities Power-Canada, LLC

  18. Structures of Adnectin/Protein Complexes Reveal an Expanded Binding Footprint

    SciTech Connect (OSTI)

    Ramamurthy, Vidhyashankar; Krystek, Jr., Stanley R.; Bush, Alexander; Wei, Anzhi; Emanuel, Stuart L.; Gupta, Ruchira Das; Janjua, Ahsen; Cheng, Lin; Murdock, Melissa; Abramczyk, Bozena; Cohen, Daniel; Lin, Zheng; Morin, Paul; Davis, Jonathan H.; Dabritz, Michael; McLaughlin, Douglas C.; Russo, Katie A.; Chao, Ginger; Wright, Martin C.; Jenny, Victoria A.; Engle, Linda J.; Furfine, Eric; Sheriff, Steven

    2014-10-02

    Adnectins are targeted biologics derived from the tenth type III domain of human fibronectin ({sup 10}Fn3), a member of the immunoglobulin superfamily. Target-specific binders are selected from libraries generated by diversifying the three {sup 10}Fn3 loops that are analogous to the complementarity determining regions of antibodies. The crystal structures of two Adnectins were determined, each in complex with its therapeutic target, EGFR or IL-23. Both Adnectins bind different epitopes than those bound by known monoclonal antibodies. Molecular modeling suggests that some of these epitopes might not be accessible to antibodies because of the size and concave shape of the antibody combining site. In addition to interactions from the Adnectin diversified loops, residues from the N terminus and/or the {beta} strands interact with the target proteins in both complexes. Alanine-scanning mutagenesis confirmed the calculated binding energies of these {beta} strand interactions, indicating that these nonloop residues can expand the available binding footprint.

  19. Beta-endorphin and alpha-n-acetyl beta-endorphin; synthesis, conformation and binding parameter

    SciTech Connect (OSTI)

    Lovegren, E.S.

    1986-01-01

    Beta-endorphin (EP) is a 31-residue opioid peptide found in many tissues, including the pituitary, brain and reproductive tract. Alpha-amino-acetyl beta-endorphin (AcEP) was characterized spectroscopically by proton nuclear magnetic resonance (NMR) and circular dichroism in deuterated water and trifluoroethanol (TFE). Both EP and AcEP bind to neuroblastoma N2a cells. This binding was not mediated through opiate receptors, and both peptides seemed to bind at common sites. Ovarian immunoreactive-EP levels were determined for immature and mature rates. These levels were found to be responsive to exogenous gonadotropin treatment in immature animals. A large percentage of the immunoreactive-EP is present in follicular fluid, and most of the endorphin-like peptides were acetylated, as measured by radioimmunoassay. Chromatogaphic analysis suggested at least three EP-like species: EP, a carboxy-terminally cleaved and an amino-terminally acetylated EP.

  20. LINC Complexes Form by Binding of Three KASH Peptides to Domain Interfaces of Trimeric SUN Proteins

    SciTech Connect (OSTI)

    Sosa, Brian A.; Rothballer, Andrea; Kutay, Ulrike; Schwartz, Thomas U.

    2012-08-31

    Linker of nucleoskeleton and cytoskeleton (LINC) complexes span the nuclear envelope and are composed of KASH and SUN proteins residing in the outer and inner nuclear membrane, respectively. LINC formation relies on direct binding of KASH and SUN in the perinuclear space. Thereby, molecular tethers are formed that can transmit forces for chromosome movements, nuclear migration, and anchorage. We present crystal structures of the human SUN2-KASH1/2 complex, the core of the LINC complex. The SUN2 domain is rigidly attached to a trimeric coiled coil that prepositions it to bind three KASH peptides. The peptides bind in three deep and expansive grooves formed between adjacent SUN domains, effectively acting as molecular glue. In addition, a disulfide between conserved cysteines on SUN and KASH covalently links both proteins. The structure provides the basis of LINC complex formation and suggests a model for how LINC complexes might arrange into higher-order clusters to enhance force-coupling.

  1. Crystal Structure of the HP1-EMSY Complex Reveals an Unusual Mode of HP1 Binding

    SciTech Connect (OSTI)

    Huang,Y.; Myers, M.; Xu, R.

    2006-01-01

    Heterochromatin protein-1 (HP1) plays an essential role in both the assembly of higher-order chromatin structure and epigenetic inheritance. The C-terminal chromo shadow domain (CSD) of HP1 is responsible for homodimerization and interaction with a number of chromatin-associated nonhistone proteins, including EMSY, which is a BRCA2-interacting protein that has been implicated in the development of breast and ovarian cancer. We have determined the crystal structure of the HP1{beta} CSD in complex with the N-terminal domain of EMSY at 1.8 Angstroms resolution. Surprisingly, the structure reveals that EMSY is bound by two HP1 CSD homodimers, and the binding sequences differ from the consensus HP1 binding motif PXVXL. This structural information expands our understanding of HP1 binding specificity and provides insights into interactions between HP1 homodimers that are likely to be important for heterochromatin formation.

  2. T-662: ISC BIND Packet Processing Flaw Lets Remote Users Deny Service

    Broader source: Energy.gov [DOE]

    A defect in the affected BIND 9 versions allows an attacker to remotely cause the "named" process to exit using a specially crafted packet. This defect affects both recursive and authoritative servers. The code location of the defect makes it impossible to protect BIND using ACLs configured within named.conf or by disabling any features at compile-time or run-time. A remote attacker would need to be able to send a specially crafted packet directly to a server running a vulnerable version of BIND. There is also the potential for an indirect attack via malware that is inadvertently installed and run, where infected machines have direct access to an organization's nameservers.

  3. Ligand-induced conformational changes in a thermophilic ribose-binding protein

    SciTech Connect (OSTI)

    Cuneo, Matthew J.; Beese, Lorena S.; Hellinga, Homme W.

    2009-05-21

    Members of the periplasmic binding protein (PBP) superfamily are involved in transport and signaling processes in both prokaryotes and eukaryotes. Biological responses are typically mediated by ligand-induced conformational changes in which the binding event is coupled to a hinge-bending motion that brings together two domains in a closed form. In all PBP-mediated biological processes, downstream partners recognize the closed form of the protein. This motion has also been exploited in protein engineering experiments to construct biosensors that transduce ligand binding to a variety of physical signals. Understanding the mechanistic details of PBP conformational changes, both global (hinge bending, twisting, shear movements) and local (rotamer changes, backbone motion), therefore is not only important for understanding their biological function but also for protein engineering experiments. Here we present biochemical characterization and crystal structure determination of the periplasmic ribose-binding protein (RBP) from the hyperthermophile Thermotoga maritima in its ribose-bound and unliganded state. The T. maritima RBP (tmRBP) has 39% sequence identity and is considerably more resistant to thermal denaturation (appTm value is 108 C) than the mesophilic Escherichia coli homolog (ecRBP) (appTm value is 56 C). Polar ligand interactions and ligand-induced global conformational changes are conserved among ecRBP and tmRBP; however local structural rearrangements involving side-chain motions in the ligand-binding site are not conserved. Although the large-scale ligand-induced changes are mediated through similar regions, and are produced by similar backbone movements in tmRBP and ecRBP, the small-scale ligand-induced structural rearrangements differentiate the mesophile and thermophile. This suggests there are mechanistic differences in the manner by which these two proteins bind their ligands and are an example of how two structurally similar proteins utilize different mechanisms to form a ligand-bound state.

  4. Binding of formyl peptides to Walker 256 carcinosarcoma cells and the chemotactic response of these cells

    SciTech Connect (OSTI)

    Rayner, D.C.; Orr, F.W.; Shiu, R.P.

    1985-05-01

    N-Formylmethionylleucylphenylalanine (fMLP) induces chemotaxis in leukocytes, the response being mediated by peptide binding to a receptor on the plasma membrane. In tumor cells, this peptide has been reported to induce cellular swelling and chemotaxis in vitro and to enhance the localization of circulating tumor cells in vivo. In the Boyden chamber, the authors evaluated the migratory responses of Walker carcinosarcoma 256 cells to varying concentrations of fMLP. Sigmoidal dose-response curves were obtained with the dose of chemotactic factor that elicits a half-maximal chemotactic response of 5.0 +/- 2.5 X 10(-8) M. Checkerboard analysis indicated that these responses were dependent upon a concentration gradient of fMLP with increases in migration of circa 2 to 2.5 times that of random movement. To examine the binding of fMLP, the tumor cells were incubated with 5 X 10(-9) M fML-(/sup 3/H)P in Hanks balanced salt solution. Specific binding (0.5 to 1% of total radioligand, to whole cells inhibited by 5 X 10(-6) M fMLP) approached equilibrium after 4 to 6 h at 4 degrees C and after 6 to 10 h at 22 degrees C. Autoradiographic studies demonstrated heterogeneous binding of the peptide by tumor cells and also showed its intracellular localization. In homogenates of Walker cells prepared in 0.1 M Tris HCl, pH 7.4, with 10 mM MgCl2 and bovine serum albumin (1 mg/ml), specific binding of approximately 0.5% of total fML-(/sup 3/H)P reached equilibrium after 60 min at 4 degrees C. In whole cells and homogenates, binding was reversible by addition of unlabeled fMLP.

  5. Boron-Lined Multitube Neutron Proportional Counter Test

    SciTech Connect (OSTI)

    Woodring, Mitchell L.; Ely, James H.; Kouzes, Richard T.; Stromswold, David C.

    2010-09-07

    Radiation portal monitors used for interdiction of illicit materials at borders include highly sensitive neutron detection systems. The main reason for having neutron detection capability is to detect fission neutrons from plutonium. The currently deployed radiation portal monitors (RPMs) from Ludlum and Science Applications International Corporation (SAIC) use neutron detectors based upon 3He-filled gas proportional counters, which are the most common large neutron detector. There is a declining supply of 3He in the world, and thus, methods to reduce the use of this gas in RPMs with minimal changes to the current system designs and sensitivity to cargo-borne neutrons are being investigated. Four technologies have been identified as being currently commercially available, potential alternative neutron detectors to replace the use of 3He in RPMs. These technologies are: 1) Boron trifluoride (BF3)-filled proportional counters, 2) Boron-lined proportional counters, 3) Lithium-loaded glass fibers, and 4) Coated non-scintillating plastic fibers. In addition, a few other companies have detector technologies that might be competitive in the near term as an alternative technology. Reported here are the results of tests of a boron-lined, “multitube” proportional counter manufactured by Centronic Ltd. (Surry, U.K. and Houston, TX). This testing measured the required performance for neutron detection efficiency and gamma-ray rejection capabilities of the detector.

  6. Polar On-Line Acquisition Relay and Transmission System (POLARATS)

    SciTech Connect (OSTI)

    Yuracko, K.

    2004-07-15

    POLARATS (Polar On-Line Acquisition Relay And Transmission System) is being developed by YAHSGS LLC (YAHSGS) and Oak Ridge National Laboratory (ORNL) to provide remote, unattended monitoring of environmental parameters under harsh environmental conditions. In particular, instrumental design and engineering is oriented towards protection of human health in the Arctic, and with the additional goal of advancing Arctic education and research. POLARATS will obtain and transmit environmental data from hardened monitoring devices deployed in locations important to understanding atmospheric and aquatic pollutant migration as it is biomagnified in Arctic food chains. An Internet- and personal computer (PC)-based educational module will provide real time sensor data, on-line educational content, and will be integrated with workbooks and textbooks for use in middle and high school science programs. The educational elements of POLARATS include an Internet-based educational module that will instruct students in the use of the data and how those data fit into changing Arctic environments and food chains. POLARATS will: (1) Enable students, members of the community, and scientific researchers to monitor local environmental conditions in real time over the Internet; and (2) Provide additional educational benefits through integration with middle- and high-school science curricula. Information will be relayed from POLARATS devices to classrooms and libraries along with custom-designed POLARATS teaching materials that will be integrated into existing curricula to enhance the educational benefits realized from the information obtained.

  7. Pulsed Neurton Elemental On-Line Material Analyzer

    DOE Patents [OSTI]

    Vourvopoulos, George (Bowling Green, KY)

    2002-08-20

    An on-line material analyzer which utilizes pulsed neutron generation in order to determine the composition of material flowing through the apparatus. The on-line elemental material analyzer is based on a pulsed neutron generator. The elements in the material interact with the fast and thermal neutrons produced from the pulsed generator. Spectra of gamma-rays produced from fast neutrons interacting with elements of the material are analyzed and stored separately from spectra produced from thermal neutron reactions. Measurements of neutron activation takes place separately from the above reactions and at a distance from the neutron generator. A primary passageway allows the material to flow through at a constant rate of speed and operators to provide data corresponding to fast and thermal neutron reactions. A secondary passageway meters the material to allow for neutron activation analysis. The apparatus also has the capability to determine the density of the flowed material. Finally, the apparatus continually utilizes a neutron detector in order to normalize the yield of the gamma ray detectors and thereby automatically calibrates and adjusts the spectra data for fluctuations in neutron generation.

  8. Polar Gas to pick route for Arctic Y Line

    SciTech Connect (OSTI)

    Not Available

    1980-05-26

    Polar Gas Project is considering four possible Y line routes to move gas reserves from the Arctic Islands and the MacKenzie Delta/Beaufort Sea areas to southern Canada. All four routes are west of the single line route proposed by Polar Gas Ltd. in 1977 to run from the Arctic Islands to Longlac, Ontario, and would connect with existing pipelines at either Longlac, Winnipeg, Calgary, or Edmonton. Marketable reserves in the High Arctic Islands are estimated at 12.7 trillion cubic feet, not counting 3-6 trillion cubic feet probably contained in recent discoveries; the MacKenzie Delta reserves are estimated at 5.8 trillion cubic feet. The gas will be chilled to 0C for passage through permafrost regions, to prevent thawing of the soil, but the gas will be at higher temperatures in other areas, with various construction techniques used to protect the area of discontinuous permafrost from thawing. More than $70 million has been spent on project studies. An application will be filed in 1981, and the pipeline could be completed in 7-10 years.

  9. Molecular cytogenetic characterization of a human thyroid cancercell line

    SciTech Connect (OSTI)

    Weier, Heinz-Ulrich G.; Tuton, Tiffany B.; Ito, Yuko; Chu, LisaW.; Lu, Chung-Mei; Baumgartner, Adolf; Zitzelsberger, Horst F.; Weier,Jingly F.

    2006-01-04

    The incidence of papillary thyroid carcinoma (PTC) increases significantly after exposure of the head and neck region to ionizing radiation, yet we know neither the steps involved in malignant transformation of thyroid epithelium nor the specific carcinogenic mode of action of radiation. Such increased tumor frequency became most evident in children after the 1986 nuclear accident in Chernobyl, Ukraine. In the twelve years following the accident, the average incidence of childhood PTCs (chPTC) increased over one hundred-fold compared to the rate of about 1 tumor incidence per 10{sup 6} children per year prior to 1986. To study the etiology of radiation-induced thyroid cancer, we formed an international consortium to investigate chromosomal changes and altered gene expression in cases of post-Chernobyl chPTC. Our approach is based on karyotyping of primary cultures established from chPTC specimens, establishment of cell lines and studies of genotype-phenotype relationships through high resolution chromosome analysis, DNA/cDNA micro-array studies, and mouse xenografts that test for tumorigenicity. Here, we report the application of fluorescence in situ hybridization (FISH)-based techniques for the molecular cytogenetic characterization of a highly tumorigenic chPTC cell line, S48TK, and its subclones. Using chromosome 9 rearrangements as an example, we describe a new approach termed ''BAC-FISH'' to rapidly delineate chromosomal breakpoints, an important step towards a better understanding of the formation of translocations and their functional consequences.

  10. The Imaging and Medical Beam Line at the Australian Synchrotron

    SciTech Connect (OSTI)

    Hausermann, Daniel; Hall, Chris; Maksimenko, Anton; Campbell, Colin [Australian Synchrotron Company, 800 Blackburn Road, Clayton, Victoria 3168 (Australia)

    2010-07-23

    As a result of the enthusiastic support from the Australian biomedical, medical and clinical communities, the Australian Synchrotron is constructing a world-class facility for medical research, the 'Imaging and Medical Beamline'. The IMBL began phased commissioning in late 2008 and is scheduled to commence the first clinical research programs with patients in 2011. It will provide unrivalled x-ray facilities for imaging and radiotherapy for a wide range of research applications in diseases, treatments and understanding of physiological processes. The main clinical research drivers are currently high resolution and sensitivity cardiac and breast imaging, cell tracking applied to regenerative and stem cell medicine and cancer therapies. The beam line has a maximum source to sample distance of 136 m and will deliver a 60 cm by 4 cm x-ray beam1 - monochromatic and white - to a three storey satellite building fully equipped for pre-clinical and clinical research. Currently operating with a 1.4 Tesla multi-pole wiggler, it will upgrade to a 4.2 Tesla device which requires the ability to handle up to 21 kW of x-ray power at any point along the beam line. The applications envisaged for this facility include imaging thick objects encompassing materials, humans and animals. Imaging can be performed in the range 15-150 keV. Radiotherapy research typically requires energies between 30 and 120 keV, for both monochromatic and broad beam.

  11. Early environmental planning: A process for power line corridor selection

    SciTech Connect (OSTI)

    Haagenstad, T.; Bare, C.M.

    1998-12-01

    Los Alamos National Laboratory (LANL) conducted an environmental planning study in the fall of 1997 to help determine the best alternative for upgrading the Laboratory`s electrical power system. Alternatives considered included an on-site power generation facility and two corridors for a 10-mile-long 115-kV power line. This planning process was conducted prior to the formal National Environmental Policy Act (NEPA) review. The goals were to help select the best proposed action, to recommend modifications and mitigation measures for each alternative for a more environmentally sound project, and to avoid potential delays once the formal Department of Energy review process began. Significant constraints existed from a planning perspective, including operational issues such as existing outdoor high explosives testing areas, as well as environmental issues including threatened and endangered species habitats, multiple archeological sites, contaminated areas, and aesthetics. The study had to be completed within 45 days to meet project schedule needs. The process resulted in a number of important recommendations. While the construction and operation of the on-site power generation facility could have minimal environmental impacts, the need for a new air quality permit would create severe cost and schedule constraints for the project. From an environmental perspective, construction and operation of a power line within either corridor was concluded to be a viable alternative. However, impacts with either corridor would have to be reduced through specific recommended alignment modifications and mitigation measures.

  12. Photoelectron Spectroscopy and Theoretical Studies of Anion-pi Interactions: Binding Strength and Anion Specificity

    SciTech Connect (OSTI)

    Zhang, Jian; Zhou, Bin; Sun, Zhenrong; Wang, Xue B.

    2015-01-01

    Proposed in theory and confirmed to exist, anion–? interactions have been recognized as new and important non-covalent binding forces. Despite extensive theoretical studies, numerous crystal structural identifications, and a plethora of solution phase investigations, intrinsic anion–? interaction strengths that are free from complications of condensed phases’ environments, have not been directly measured in the gas phase. Herein we present a joint photoelectron spectroscopic and theoretical study on this subject, in which tetraoxacalix[2]arene[2]triazine 1, an electron-deficient and cavity self-tunable macrocyclic was used as a charge-neutral molecular host to probe its interactions with a series of anions with distinctly different shapes and charge states (spherical halides Cl?, Br?, I?, linear thiocyanate SCN?, trigonal planar nitrate NO??, pyramidic iodate IO??, and tetrahedral sulfate SO?²?). The binding energies of the resultant gaseous 1:1 complexes (1•Cl?,1•Br?, 1•I?, 1•SCN?, 1•NO??, 1•IO?? and 1•SO?²?) were directly measured experimentally, exhibiting substantial non-covalent interactions with pronounced anion specific effects. The binding strengths of Cl?, NO??, IO?? with 1 are found to be strongest among all singly charged anions, amounting to ca. 30 kcal/mol, but only about 40% of that between 1 and SO?²?. Quantum chemical calculations reveal that all anions reside in the center of the cavity of 1 with anion–? binding motif in the complexes’ optimized structures, where 1 is seen to be able to self-regulate its cavity structure to accommodate anions of different geometries and three-dimensional shapes. Electron density surface and natural bond orbital charge distribution analysis further support anion–? binding formation. The calculated binding energies of the anions and 1 nicely reproduce the experimentally estimated electron binding energy increase. This work illustrates that size-selective photoelectron spectroscopy combined with theoretical calculations represent a powerful technique to probe intrinsic anion–? interactions and has potential to provide quantitative guest-host molecular binding strengths and unravel fundamental insights in specific anion recognitions.

  13. De novo Design of an Artificial bis-[4Fe4S] Binding Protein

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    De novo Design of an Artificial bis-[4Fe4S] Binding Protein Authors: Roy, A,, Sarrou, I., Vaughn, M.D., Astashkin, A.V., and Ghirlanda, G. Title: De novo Design of an Artificial bis-[4Fe4S] Binding Protein Source: Biochemistry Year: 2013 Volume: 52 Pages: 7586-7594 ABSTRACT: In nature, protein subunits containing multiple iron-sulfur clusters often mediate the delivery of reducing equivalents from metabolic pathways to the active site of redox proteins. The de novo design of redox active

  14. Structural Basis of Wnt Signaling Inhibition by Dickkopf Binding to LRP5/6

    Office of Scientific and Technical Information (OSTI)

    (Journal Article) | SciTech Connect Basis of Wnt Signaling Inhibition by Dickkopf Binding to LRP5/6 Citation Details In-Document Search Title: Structural Basis of Wnt Signaling Inhibition by Dickkopf Binding to LRP5/6 Authors: Ahn, Victoria E. ; Chu, Matthew Ling-Hon ; Choi, Hee-Jung ; Tran, Denise ; Abo, Arie ; Weis, William I. Publication Date: 2011-11-01 OSTI Identifier: 1198118 Type: Published Article Journal Name: Developmental Cell Additional Journal Information: Journal Volume: 21;

  15. Structural Studies of Wnts and Identification of an LRP6 Binding Site

    Office of Scientific and Technical Information (OSTI)

    (Journal Article) | SciTech Connect Structural Studies of Wnts and Identification of an LRP6 Binding Site Citation Details In-Document Search Title: Structural Studies of Wnts and Identification of an LRP6 Binding Site Authors: Chu, Matthew Ling-Hon ; Ahn, Victoria E. ; Choi, Hee-Jung ; Daniels, Danette L. ; Nusse, Roel ; Weis, William I. ; , Publication Date: 2014-05-13 OSTI Identifier: 1131442 Report Number(s): SLAC-REPRINT-2014-111 DOE Contract Number: AC02-76SF00515 Resource Type:

  16. Structure and Ca[superscript 2+]-Binding Properties of the Tandem

    Office of Scientific and Technical Information (OSTI)

    C[subscript 2] Domains of E-Syt2 (Journal Article) | SciTech Connect and Ca[superscript 2+]-Binding Properties of the Tandem C[subscript 2] Domains of E-Syt2 Citation Details In-Document Search Title: Structure and Ca[superscript 2+]-Binding Properties of the Tandem C[subscript 2] Domains of E-Syt2 Authors: Xu, Junjie ; Bacaj, Taulant ; Zhou, Amy ; Tomchick, Diana R. ; Südhof, Thomas C. ; Rizo, Josep [1] ; UTSMC) [2] + Show Author Affiliations (Stanford-MED) ( Publication Date: 2014-12-23

  17. Syntaxin 1a Variants Lacking an N-peptide or Bearing the LE Mutation Bind

    Office of Scientific and Technical Information (OSTI)

    to Munc18a in a Closed Conformation (Journal Article) | SciTech Connect Syntaxin 1a Variants Lacking an N-peptide or Bearing the LE Mutation Bind to Munc18a in a Closed Conformation Citation Details In-Document Search Title: Syntaxin 1a Variants Lacking an N-peptide or Bearing the LE Mutation Bind to Munc18a in a Closed Conformation Authors: Colbert, Karen N. ; Hattendorf, Douglas A. ; Weiss, Thomas M. ; Burkhardt, Pawel ; Fasshauer, Dirk ; Weis, William I. Publication Date: 2014-05-13 OSTI

  18. Fuel Cell Transit Buses: ThunderPower Bus Evaluation at SunLine...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Buses: ThunderPower Bus Evaluation at SunLine Transit Agency Fuel Cell Transit Buses: ThunderPower Bus Evaluation at SunLine Transit Agency Report details the six-month evaluation...

  19. Hard x-ray delay line for x-ray photon correlation spectroscopy...

    Office of Scientific and Technical Information (OSTI)

    Hard x-ray delay line for x-ray photon correlation spectroscopy and jitter-free pump-probe experiments at LCLS Citation Details In-Document Search Title: Hard x-ray delay line for...

  20. Unusual emission lines of carbon in the 170-190 A region on NSTX...

    Office of Scientific and Technical Information (OSTI)

    Unusual emission lines of carbon in the 170-190 A region on NSTX Citation Details In-Document Search Title: Unusual emission lines of carbon in the 170-190 A region on NSTX You...