National Library of Energy BETA

Sample records for binds dashed line

  1. Dashed lines relationships

    E-Print Network [OSTI]

    2 MAG1 DUN1DIN7 GPG1 RFA2 HOR7 NAB2 TPS1 YOL155C YER079W YHR138C YFR017C YLR297W YBR053C YPR015C YIL

  2. A Dash of Salt 

    E-Print Network [OSTI]

    Supercinski, Danielle

    2006-01-01

    stream_source_info A dash of salt.pdf.txt stream_content_type text/plain stream_size 9159 Content-Encoding ISO-8859-1 stream_name A dash of salt.pdf.txt Content-Type text/plain; charset=ISO-8859-1 tx H2O | pg. 18 A... Texas A&M researcher is assessing the impact of using moderately saline water for irrigating urban landscapes in West Texas and southern New Mexico. A DASH OF SALT Researcher assesses salinity impacts on grasses, trees and shrubs A Dash of Salt...

  3. Dash: Improving Community Repositories for Better Data Sharing

    E-Print Network [OSTI]

    Strasser, Carly; Cruse, Patricia; Abrams, Stephen

    2014-01-01

    Tail of Science.  Library Trends 57(2) Fall 2008.   Dash:Preservation Program." Library Trends, 57:3 (2009): 301-314.

  4. Demonstration of Datacenter Automation Software and Hardware (DASH) at the California Franchise Tax Board

    E-Print Network [OSTI]

    Bell, Geoffrey C.

    2010-01-01

    of Datacenter Automation Software and Hardware (DASH) at theof Datacenter Automation Software and Hardware (DASH) at theprotocol for building automation and control networks. It is

  5. Particle trap to sheath non-binding contact for a gas-insulated transmission line having a corrugated outer conductor

    DOE Patents [OSTI]

    Fischer, William H. (Pittsburgh, PA)

    1984-04-24

    A non-binding particle trap to outer sheath contact for use in gas insulated transmission lines having a corrugated outer conductor. The non-binding feature of the contact according to the teachings of the invention is accomplished by having a lever arm rotatably attached to a particle trap by a pivot support axis disposed parallel to the direction of travel of the inner conductor/insulator/particle trap assembly.

  6. Exciton and biexciton dynamics in single self-assembled InAs/InGaAlAs/InP quantum dash emitting near 1.55??m

    SciTech Connect (OSTI)

    Dusanowski, ?.; Syperek, M. Rudno-Rudzi?ski, W.; Mrowi?ski, P.; Sek, G.; Misiewicz, J.; Somers, A.; Reithmaier, J. P.; Höfling, S.; Forchel, A.

    2013-12-16

    Exciton and biexciton dynamics in a single self-assembled InAs/In{sub 0.53}Ga{sub 0.23}Al{sub 0.24}As/InP(001) quantum dash emitting near 1.55??m has been investigated by micro-photoluminescence and time-resolved micro-photoluminescence at T?=?4.2?K. The exciton and biexciton fine structure splitting of ?60??eV, the biexciton binding energy of ?3.5?meV, and the characteristic exciton and biexciton decay times of 2.0?±?0.1?ns and 1.1?±?0.1?ns, respectively, have been determined. The measurement of the biexciton and exciton cross-correlation statistics of the photon emission confirmed the cascaded relaxation process. The exciton-to-biexciton decay time ratio and a small fine structure splitting suggest carrier localization within the investigated quantum dash.

  7. Tensile GaAs(111) quantum dashes with tunable luminescence below the bulk bandgap

    SciTech Connect (OSTI)

    Yerino, Christopher D., E-mail: christopher.yerino@yale.edu; Jung, Daehwan; Lee, Minjoo Larry [Department of Electrical Engineering, Yale University, New Haven, Connecticut 06520 (United States); Simmonds, Paul J.; Liang, Baolai [California NanoSystems Institute, University of California, Los Angeles, California 90095 (United States); Dorogan, Vitaliy G.; Ware, Morgan E.; Mazur, Yuriy I.; Salamo, Gregory J. [Institute for Nanoscience and Engineering, University of Arkansas, Fayetteville, Arkansas 72701 (United States); Huffaker, Diana L. [California NanoSystems Institute, University of California, Los Angeles, California 90095 (United States); Department of Electrical Engineering, University of California, Los Angeles, California 90095 (United States)

    2014-08-18

    Strain-based band engineering in quantum dots and dashes has been predominantly limited to compressively strained systems. However, tensile strain strongly reduces the bandgaps of nanostructures, enabling nanostructures to emit light at lower energies than they could under compressive strain. We demonstrate the self-assembled growth of dislocation-free GaAs quantum dashes on an InP(111)B substrate, using a 3.8% tensile lattice-mismatch. Due to the high tensile strain, the GaAs quantum dashes luminesce at 110–240?meV below the bandgap of bulk GaAs. The emission energy is readily tuned by adjusting the size of the quantum dashes via deposition thickness. Tensile self-assembly creates new opportunities for engineering the band alignment, band structure, and optical properties of epitaxial nanostructures.

  8. Optical injection modulation of quantum-dash semiconductor lasers by intra-cavity stimulated

    E-Print Network [OSTI]

    .5960) Semiconductor lasers; (190.5650) Raman effect; (250.5590) quantum- well, -wire and -dot devices. References-temperature operation of InAs quantum-dash lasers on InP (001)," IEEE Photon. Technol. Lett. 13(8), 767­769 (2001). 2. D. Loualiche, "Low-threshold current density InAs quantum dash lasers on InP (100) grown by molecular beam

  9. Non-binding conductor load bearing roller for a gas-insulated transmission line having a corrugated outer conductor

    DOE Patents [OSTI]

    Fischer, William H. (Pittsburgh, PA)

    1984-01-01

    A gas-insulated transmission line includes a corrugated outer conductor, an inner conductor disposed within and insulated from the outer conductor by means of support insulators and an insulating gas, and a non-binding transport device for supporting and permitting movement of the inner conductor/insulating support assembly axially along the corrugated outer conductor without radial displacement and for moving without binding along corrugations of any slope less than vertical. The transport device includes two movable contacts, such as skids or rollers, supported on a common pivot lever, the pivot lever being rotatably disposed about a pivot lever axis, which pivot lever axis is in turn disposed on the periphery of a support insulator or particle trap if one is used. The movable contacts are separated axially a distance equal to the axial distance between the peaks and valleys of the corrugations of the outer conductor and separated radially a distance equal to the radial distance between the peaks and valleys of the corrugations of the outer conductor. The transport device has the pivot lever axis disposed parallel to the motion of travel of the inner conductor/insulating support assembly.

  10. Broadband emission in InAs/InGaAlAs quantum-dash-in-well laser Boon S. Ooi1, a

    E-Print Network [OSTI]

    : Quantum dash, Quantum dot, Broadband emission, Semiconductor Laser. Abstract. We report on the developmentBroadband emission in InAs/InGaAlAs quantum-dash-in-well laser Boon S. Ooi1, a , Hery S. Djie1 of wide gain InAs/InGaAlAs/InP quantum-dash structure for broadband diode laser and amplifier

  11. Jenny and Claire dashed across the school perimeter line together. The hated school

    E-Print Network [OSTI]

    Loss, Daniel

    for the real world outside school. But it was three days old. Claire's was four days old. Jenny let her eyes dress every day. Any adult woman with even a hint of style wore at least two different outfits a day. E-fashion changed as fast as the news and old e-fashion was as interesting as old news. "This might as well be five

  12. Single photon emission at 1.55??m from charged and neutral exciton confined in a single quantum dash

    SciTech Connect (OSTI)

    Dusanowski, ?. Syperek, M.; Mrowi?ski, P.; Rudno-Rudzi?ski, W.; Misiewicz, J.; S?k, G.; Somers, A.; Kamp, M.; Höfling, S.; Reithmaier, J. P.

    2014-07-14

    We investigate charged and neutral exciton complexes confined in a single self-assembled InAs/InGaAlAs/InP quantum dash emitting at 1.55??m. The emission characteristics have been probed by measuring high-spatial-resolution polarization-resolved photoluminescence and cross-correlations of photon emission statistics at T?=?5?K. The photon auto-correlation histogram of the emission from both the neutral and charged exciton indicates a clear antibunching dip with as-measured g{sup (2)}(0) values of 0.18 and 0.31, respectively. It proves that these exciton complexes confined in single quantum dashes of InP-based material system can act as true single photon emitters being compatible with standard long-distance fiber communication technology.

  13. DASH | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIX E LISTStar EnergyLawler,CoalConcordiaConsumerLEDSEnergy Information aka

  14. Cellulose binding domain proteins

    DOE Patents [OSTI]

    Shoseyov, Oded (Karmey Yosef, IL); Shpiegl, Itai (Rehovot, IL); Goldstein, Marc (Davis, CA); Doi, Roy (Davis, CA)

    1998-01-01

    A cellulose binding domain (CBD) having a high affinity for crystalline cellulose and chitin is disclosed, along with methods for the molecular cloning and recombinant production thereof. Fusion products comprising the CBD and a second protein are likewise described. A wide range of applications are contemplated for both the CBD and the fusion products, including drug delivery, affinity separations, and diagnostic techniques.

  15. Cellulose binding domain proteins

    DOE Patents [OSTI]

    Shoseyov, O.; Shpiegl, I.; Goldstein, M.; Doi, R.

    1998-11-17

    A cellulose binding domain (CBD) having a high affinity for crystalline cellulose and chitin is disclosed, along with methods for the molecular cloning and recombinant production. Fusion products comprising the CBD and a second protein are likewise described. A wide range of applications are contemplated for both the CBD and the fusion products, including drug delivery, affinity separations, and diagnostic techniques. 16 figs.

  16. Molecular Defects in Variant Forms of Mannose-Binding Protein Associated with Immunodeficiency1

    E-Print Network [OSTI]

    of the first lines of defense against invasion by pathogenic microorganisms (1, 2). MBPs bind to sugar to generate fragments that have C3- convertase activity (3­8). Microorganisms are neutralized either

  17. How Plasmodium falciparum malaria parasites bind to human brain endothelial cells 

    E-Print Network [OSTI]

    Claessens, Antoine

    2011-01-01

    Cerebral malaria is characterised by an accumulation of infected erythrocytes in the microvasculature of the brain. Plasmodium falciparum infected erythrocytes have been shown to bind to a Human Brain Endothelial Cell line (HBEC-5i) in vitro...

  18. Hemodynamic Monitoring TRANSTHORACIC LINES

    E-Print Network [OSTI]

    Kay, Mark A.

    and with position changes. 5. All lines should have either heparinized saline or D5W infusing. 6. Avoid infusing the line. 9. Never use LA for infusions or bolus medications. 10. Vigilant observation for AIR. B. Line

  19. Plains and Eastern Clean Line Transmission Line: Comment from...

    Energy Savers [EERE]

    Plains and Eastern Clean Line Transmission Line: Comment from Marshall Hughes Plains and Eastern Clean Line Transmission Line: Comment from Marshall Hughes Comment submitted on...

  20. Plains and Eastern Clean Line Transmission Line: Comment from...

    Office of Environmental Management (EM)

    from Block Plains and Eastern Clean Line: Arkansas and Oklahoma Plains and Eastern Clean Line Transmission Line: Comment from Block Plains and Eastern Clean Line: Arkansas and...

  1. December 21, 2012 4:58 World Scientific Review Volume -9in x 6in andereggInternalTransport Internal Transport in Non-neutral Plasma

    E-Print Network [OSTI]

    California at San Diego, University of

    - perature T has unit of energy. The case of interest D > rc corresponds to low density plasma in a large collision. The impact parameter green dashed lines. Figure 1 range E × B drift collisions; the impact green dashed lines

  2. Racing line optimization

    E-Print Network [OSTI]

    Xiong, Ying, S.M. Massachusetts Institute of Technology

    2010-01-01

    Although most racers are good at controlling their cars, world champions are always talented at choosing the right racing line while others mostly fail to do that. Optimal racing line selection is a critical problem in car ...

  3. Synthetic heparin-binding growth factor analogs

    DOE Patents [OSTI]

    Pena, Louis A.; Zamora, Paul; Lin, Xinhua; Glass, John D.

    2007-01-23

    The invention provides synthetic heparin-binding growth factor analogs having at least one peptide chain that binds a heparin-binding growth factor receptor, covalently bound to a hydrophobic linker, which is in turn covalently bound to a non-signaling peptide that includes a heparin-binding domain. The synthetic heparin-binding growth factor analogs are useful as soluble biologics or as surface coatings for medical devices.

  4. Finite Field Arithmetic and its Application in Cryptography

    E-Print Network [OSTI]

    Ansari, Bijan

    2012-01-01

    k versus Critical Paths . . . . . . . . . . . . . . . . .The dash-dot line indicates the critical path for the errorwhere critical path is highlighted. . . . . . . . . . . . .

  5. Mad Dash Inc | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIXsource HistoryScenarios Towards 2050EnermarGeneration Jump to: navigation, search

  6. Bounds to binding energies from the concavity of thermodynamical functions

    E-Print Network [OSTI]

    B. K. Jennings; B. R. Barrett; B. G. Giraud

    2007-08-22

    Sequences of experimental ground-state energies are mapped onto concave patterns cured from convexities due to pairing and/or shell effects. The same patterns, completed by a list of excitation energies, can be used to give numerical estimates of the grand potential $\\Omega(\\beta,\\mu)$ for a mixture of nuclei at low or moderate temperatures $T=\\beta^{-1}$ and at many chemical potentials $\\mu.$ The average nucleon number $(\\beta,\\mu)$ then becomes a continuous variable, allowing extrapolations towards nuclear masses closer to drip lines. We study the possible concavity of several thermodynamical functions, such as the free energy and the average energy, as functions of $.$ Concavity, when present in such functions, allows trivial interpolations and extrapolations providing upper and lower bounds, respectively, to binding energies. Such bounds define an error bar for the prediction of binding energies. An extrapolation scheme for such concave functions is tested. We conclude with numerical estimates of the binding energies of a few nuclei closer to drip lines.

  7. Cellulose binding domain fusion proteins

    DOE Patents [OSTI]

    Shoseyov, O.; Yosef, K.; Shpiegl, I.; Goldstein, M.A.; Doi, R.H.

    1998-02-17

    A cellulose binding domain (CBD) having a high affinity for crystalline cellulose and chitin is disclosed, along with methods for the molecular cloning and recombinant production. Fusion products comprising the CBD and a second protein are likewise described. A wide range of applications are contemplated for both the CBD and the fusion products, including drug delivery, affinity separations, and diagnostic techniques. 16 figs.

  8. Cellulose binding domain fusion proteins

    DOE Patents [OSTI]

    Shoseyov, Oded (Karmey Yosef, IL); Shpiegl, Itai (Rehovot, IL); Goldstein, Marc A. (Davis, CA); Doi, Roy H. (Davis, CA)

    1998-01-01

    A cellulose binding domain (CBD) having a high affinity for crystalline cellulose and chitin is disclosed, along with methods for the molecular cloning and recombinant production thereof. Fusion products comprising the CBD and a second protein are likewise described. A wide range of applications are contemplated for both the CBD and the fusion products, including drug delivery, affinity separations, and diagnostic techniques.

  9. Series Transmission Line Transformer

    DOE Patents [OSTI]

    Buckles, Robert A. (Livermore, CA); Booth, Rex (Livermore, CA); Yen, Boris T. (El Cerrito, CA)

    2004-06-29

    A series transmission line transformer is set forth which includes two or more of impedance matched sets of at least two transmissions lines such as shielded cables, connected in parallel at one end ans series at the other in a cascading fashion. The cables are wound about a magnetic core. The series transmission line transformer (STLT) which can provide for higher impedance ratios and bandwidths, which is scalable, and which is of simpler design and construction.

  10. Transmission Line Security Monitor

    ScienceCinema (OSTI)

    None

    2013-05-28

    The Transmission Line Security Monitor is a multi-sensor monitor that mounts directly on high-voltage transmission lines to detect, characterize and communicate terrorist activity, human tampering and threatening conditions around support towers. For more information about INL's critical infrastructure protection research, visit http://www.facebook.com/idahonationallaboratory.

  11. Transmission Line Security Monitor

    SciTech Connect (OSTI)

    2011-01-01

    The Transmission Line Security Monitor is a multi-sensor monitor that mounts directly on high-voltage transmission lines to detect, characterize and communicate terrorist activity, human tampering and threatening conditions around support towers. For more information about INL's critical infrastructure protection research, visit http://www.facebook.com/idahonationallaboratory.

  12. Erythropoietin binding protein from mammalian serum

    DOE Patents [OSTI]

    Clemons, Gisela K. (Berkeley, CA)

    1997-01-01

    Purified mammalian erythropoietin binding-protein is disclosed, and its isolation, identification, characterization, purification, and immunoassay are described. The erythropoietin binding protein can be used for regulation of erythropoiesis by regulating levels and half-life of erythropoietin. A diagnostic kit for determination of level of erythropoietin binding protein is also described.

  13. Erythropoietin binding protein from mammalian serum

    DOE Patents [OSTI]

    Clemons, G.K.

    1997-04-29

    Purified mammalian erythropoietin binding-protein is disclosed, and its isolation, identification, characterization, purification, and immunoassay are described. The erythropoietin binding protein can be used for regulation of erythropoiesis by regulating levels and half-life of erythropoietin. A diagnostic kit for determination of level of erythropoietin binding protein is also described. 11 figs.

  14. Climate change: Clinton affirms binding emissions reduction policy

    SciTech Connect (OSTI)

    Fairley, P.

    1996-12-04

    In Australia last month President Clinton called for an international agreement to negotiate {open_quotes}legally binding commitments to fight climate change.{close_quotes} His comments affirmed the line the Administration adopted in July and lent prominence to the effort to bring about a treaty by December 1997. Environmentalists welcomed Clinton`s comments, but industry response is divided. The Global Climate Coalition (Washington), of which CMA is a member, has tried to slow negotiations by questioning the scientific consensus on climate change and suggesting {open_quotes}serious damage to the American economy{close_quotes} could result from emissions reduction.

  15. A Rare Earth-DOTA-Binding Antibody: Probe Properties and Binding Affinity across the Lanthanide Series

    E-Print Network [OSTI]

    Fisher, Andrew J.

    1) binds transition metals and rare earths with extreme stability under physiological conditionsA Rare Earth-DOTA-Binding Antibody: Probe Properties and Binding Affinity across the Lanthanide affinity and exquisite specificity.1 An antibody that binds rare earth complexes selectively could be used

  16. Synthetic heparin-binding factor analogs

    DOE Patents [OSTI]

    Pena, Louis A. (Poquott, NY); Zamora, Paul O. (Gaithersburg, MD); Lin, Xinhua (Plainview, NY); Glass, John D. (Shoreham, NY)

    2010-04-20

    The invention provides synthetic heparin-binding growth factor analogs having at least one peptide chain, and preferably two peptide chains branched from a dipeptide branch moiety composed of two trifunctional amino acid residues, which peptide chain or chains bind a heparin-binding growth factor receptor and are covalently bound to a non-signaling peptide that includes a heparin-binding domain, preferably by a linker, which may be a hydrophobic linker. The synthetic heparin-binding growth factor analogs are useful as pharmaceutical agents, soluble biologics or as surface coatings for medical devices.

  17. Coherent soliton communication lines

    SciTech Connect (OSTI)

    Yushko, O. V. Redyuk, A. A.; Fedoruk, M. P.; Turitsyn, S. K.

    2014-11-15

    The data transmission in coherent fiber-optical communication lines using solitons with a variable phase is studied. It is shown that nonlinear coherent structures (solitons) can be applied for effective signal transmission over a long distance using amplitude and optical-phase keying of information. The optimum ratio of the pulse width to the bit slot at which the spectral efficiency (transmitted bits per second and hertz) is maximal is determined. It is shown that soliton fiber-optical communication lines can ensure data transmission at a higher spectral efficiency as compared to traditional communication lines and at a high signal-to-noise ratio.

  18. Power line detection system

    DOE Patents [OSTI]

    Latorre, V.R.; Watwood, D.B.

    1994-09-27

    A short-range, radio frequency (RF) transmitting-receiving system that provides both visual and audio warnings to the pilot of a helicopter or light aircraft of an up-coming power transmission line complex. Small, milliwatt-level narrowband transmitters, powered by the transmission line itself, are installed on top of selected transmission line support towers or within existing warning balls, and provide a continuous RF signal to approaching aircraft. The on-board receiver can be either a separate unit or a portion of the existing avionics, and can also share an existing antenna with another airborne system. Upon receipt of a warning signal, the receiver will trigger a visual and an audio alarm to alert the pilot to the potential power line hazard. 4 figs.

  19. Transmission line capital costs

    SciTech Connect (OSTI)

    Hughes, K.R.; Brown, D.R.

    1995-05-01

    The displacement or deferral of conventional AC transmission line installation is a key benefit associated with several technologies being developed with the support of the U.S. Department of Energy`s Office of Energy Management (OEM). Previous benefits assessments conducted within OEM have been based on significantly different assumptions for the average cost per mile of AC transmission line. In response to this uncertainty, an investigation of transmission line capital cost data was initiated. The objective of this study was to develop a database for preparing preliminary estimates of transmission line costs. An extensive search of potential data sources identified databases maintained by the Bonneville Power Administration (BPA) and the Western Area Power Administration (WAPA) as superior sources of transmission line cost data. The BPA and WAPA data were adjusted to a common basis and combined together. The composite database covers voltage levels from 13.8 to 765 W, with cost estimates for a given voltage level varying depending on conductor size, tower material type, tower frame type, and number of circuits. Reported transmission line costs vary significantly, even for a given voltage level. This can usually be explained by variation in the design factors noted above and variation in environmental and land (right-of-way) costs, which are extremely site-specific. Cost estimates prepared from the composite database were compared to cost data collected by the Federal Energy Regulatory Commission (FERC) for investor-owned utilities from across the United States. The comparison was hampered because the only design specifications included with the FERC data were voltage level and line length. Working within this limitation, the FERC data were not found to differ significantly from the composite database. Therefore, the composite database was judged to be a reasonable proxy for estimating national average costs.

  20. Substrate masking: binding of RNA by EGTA-inactivated micrococcal nuclease results in artifactual inhibition of RNA processing reactions

    E-Print Network [OSTI]

    Wang, Ming Jing; Gegenheimer, Peter Albert

    1991-01-01

    -directed mutagenesis ofMN (2) has recently allowed separate identification of residues involved in metal binding, in nucleic acid binding, or in catalysis. These studies together led to a catalytic mechanism which involves nucleophilic attack on phosphorus by a water... of the same data; the solid line is a plot of Eqn. II (Materials and Methods) using the constants derived above. reduction of nitrocellulose filter-bound pre-tRNA. From the IC50 values (concentration giving 50% inhibition of binding) of these compounds, we can...

  1. Expression levels of MHC class I molecules are inversely correlated with promiscuity of peptide binding

    E-Print Network [OSTI]

    Chappell, Paul E.; Meziane, El Kahina; Harrison, Michael; Magiera, ?ukasz; Hermann, Clemens; Mears, Laura; Wrobel, Antoni G.; Durant, Charlotte; Nielsen, Lise Lotte; Buus, Søren; Ternette, Nicola; Mwangi, William; Butter, Colin; Nair, Venugopal; Ahyee, Trudy; Duggleby, Richard; Madrigal, Alejandro; Roversi, Pietro; Lea, Susan M.; Kaufman, Jim

    2015-04-10

    in and around the beginning of the groove and reveals Figure 4. Structures of BF2*2101 with different peptides show several modes of promiscuous binding through remodelling of the binding site. Left panels, top down view with peptide as sticks (N... residues. Upper panels, side view from ?2 domain side with peptide as sticks (N-terminus of peptide to the left; carbon atoms of peptide, yellow; carbon atoms of class I molecule, white; nitrogen atoms, blue; oxygen atoms, red; hydrogen bonds, dotted lines...

  2. Docking Small Ligands in Flexible Binding Sites

    E-Print Network [OSTI]

    Caflisch, Amedeo

    binding site. For all three systems the best minima in terms of free energy have a root mean square 3100-043423.95 Contractrgrant sponsor: Swiss Federal Office of Public Health; contractrgrant number the conformational space that has to be searched. There are known examples where the empty receptor cannot bind

  3. Printed circuit dispersive transmission line

    DOE Patents [OSTI]

    Ikezi, H.; Lin-Liu, Y.R.; DeGrassie, J.S.

    1991-08-27

    A printed circuit dispersive transmission line structure is disclosed comprising an insulator, a ground plane formed on one surface of the insulator, a first transmission line formed on a second surface of the insulator, and a second transmission line also formed on the second surface of the insulator and of longer length than the first transmission line and periodically intersecting the first transmission line. In a preferred embodiment, the transmission line structure exhibits highly dispersive characteristics by designing the length of one of the transmission line between two adjacent periodic intersections to be longer than the other. 5 figures.

  4. Printed circuit dispersive transmission line

    DOE Patents [OSTI]

    Ikezi, Hiroyuki (Rancho Santa Fe, CA); Lin-Liu, Yuh-Ren (San Diego, CA); DeGrassie, John S. (Encinitas, CA)

    1991-01-01

    A printed circuit dispersive transmission line structure is disclosed comprising an insulator, a ground plane formed on one surface of the insulator, a first transmission line formed on a second surface of the insulator, and a second transmission line also formed on the second surface of the insulator and of longer length than the first transmission line and periodically intersecting the first transmission line. In a preferred embodiment, the transmission line structure exhibits highly dispersive characteristics by designing the length of one of the transmission line between two adjacent periodic intersections to be longer than the other.

  5. Spectral characterization of Ekman velocities in the Southern Ocean based on surface drifter trajectories

    E-Print Network [OSTI]

    Elipot, Shane

    2006-01-01

    stress ocean velocity crosswind component downwind componentsolid lines) and the crosswind components (dashed lines).for the imaginary part (crosswind component). z such, they

  6. The Sahelian Climate

    E-Print Network [OSTI]

    Xue, Y; et al.,

    2012-01-01

    run (solid line) and desertification run (dashed line); bmean from 1951–1970; b desertification run and observed meana much more dramatic desertification episode than that which

  7. Drill string transmission line

    DOE Patents [OSTI]

    Hall, David R. (Provo, UT); Hall, Jr., H. Tracy (Provo, UT); Pixton, David S. (Lehi, UT); Bradford, Kline (Orem, UT); Fox, Joe (Spanish Fork, UT)

    2006-03-28

    A transmission line assembly for transmitting information along a downhole tool comprising a pin end, a box end, and a central bore traveling between the pin end and the box end, is disclosed in one embodiment of the invention as including a protective conduit. A transmission line is routed through the protective conduit. The protective conduit is routed through the central bore and the ends of the protective conduit are routed through channels formed in the pin end and box end of the downhole tool. The protective conduit is elastically forced into a spiral or other non-linear path along the interior surface of the central bore by compressing the protective conduit to a length within the downhole tool shorter than the protective conduit.

  8. Improved flow cytometer measurement of binding assays

    DOE Patents [OSTI]

    Saunders, G.C.

    1984-05-30

    The invention relates to a method of measuring binding assays carried out with different size particles wherein the binding assay sample is run through a flow cytometer without separating the sample from the marking agent. The amount of a binding reactant present in a sample is determined by providing particles with a coating of binder and also a known quantity of smaller particles with a coating of binder reactant. The binding reactant is the same as the binding reactant present in the sample. The smaller particles also contain a fluorescent chemical. The particles are combined with the sample and the binding reaction is allowed to occur for a set length of time followed by combining the smaller particles with the mixture of the particles and the sample produced and allowing the binding reactions to proceed to equilibrium. The fluorescence and light scatter of the combined mixture is then measured as the combined mixture passes through a flow cytometer equipped with a laser to bring about fluorescence, and the number and strength of fluorescent events are compared. A similar method is also provided for determining the amount of antigen present in the sample by providing spheres with an antibody coating and some smaller spheres with an antigen coating. (LEW)

  9. Bipolaron and N-Polaron Binding Energies

    SciTech Connect (OSTI)

    Frank, Rupert L. [Department of Mathematics, Princeton University, Washington Road, Princeton, New Jersey 08544 (United States); Lieb, Elliott H. [Department of Mathematics, Princeton University, Washington Road, Princeton, New Jersey 08544 (United States); Department of Physics, Princeton University, P.O. Box 708, Princeton, New Jersey 08542 (United States); Seiringer, Robert [Department of Physics, Princeton University, P.O. Box 708, Princeton, New Jersey 08542 (United States); Thomas, Lawrence E. [Department of Mathematics, University of Virginia, Charlottesville, Virginia 22904 (United States)

    2010-05-28

    The binding of polarons, or its absence, is an old and subtle topic. Here we prove two things rigorously. First, the transition from many-body collapse to the existence of a thermodynamic limit for N polarons occurs precisely at U=2{alpha}, where U is the electronic Coulomb repulsion and {alpha} is the polaron coupling constant. Second, if U is large enough, there is no multipolaron binding of any kind. Considering the known fact that there is binding for some U>2{alpha}, these conclusions are not obvious and their proof has been an open problem for some time.

  10. Biochem. J. (2002) 362, 265271 (Printed in Great Britain) 265 The transfer of transthyretin and receptor-binding properties from

    E-Print Network [OSTI]

    van Aalten, Daan

    2002-01-01

    SIVAPRASADARAO *School of Biochemistry and Molecular Biology, University of Leeds, Leeds LS2 9JT, U.K., and School of Biomedical Sciences, University of Leeds, Leeds LS2 9JT, U.K. Members of the lipocalin (labelled A­H; Figure 1). The interior of the barrel, known as the retinol-binding pocket, is lined

  11. Sequestering Uranium from Seawater: Binding Strength and Modes...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Sequestering Uranium from Seawater: Binding Strength and Modes of Uranyl Complexes with Glutarimidedioxime Sequestering Uranium from Seawater: Binding Strength and Modes of Uranyl...

  12. Holes in Spectral Lines

    E-Print Network [OSTI]

    Fontana, Peter R.; Srivastava, Rajendra P.

    1973-06-01

    at E = 0 is 2le I' Ib/(t)I = @~ R~R~~»nh'(IRlyt)e"'" (13)a ylal 0 5 '7 FIG. 3. Probabilities of photon emission as a function of time. The frequency corresponds to the energy differ- ence between the unperturbed degenerate excited states and the ground... states 6 is 0. 5 ey. For V= 0 the emission line is Lorentzian, but for V0 a "hole" appears at the frequency equal to the frequency difference between the excited nondecay- ing state and the ground state. The position of the "hole" is independent...

  13. The Line Verifier GVERIFY1

    E-Print Network [OSTI]

    Griffith, Arnold K.

    1971-01-01

    A line verifier is presented which, given the co-ordinates of the end points of the hypothesized line, returns a (possibly) more accurate version of the end points, together with an estimate of the probability that there ...

  14. Metal-binding polymesr as chelating agents

    E-Print Network [OSTI]

    Mohammadi, Zahra

    2011-04-11

    , high affinity binding of toxic metals by these functionalized hydrogels offers potential applications in waste water treatment and may enable applications in acute metal poisoning. Finally, a unique synthetic methodology using similar metal chelating...

  15. Hardware device binding and mutual authentication

    DOE Patents [OSTI]

    Hamlet, Jason R; Pierson, Lyndon G

    2014-03-04

    Detection and deterrence of device tampering and subversion by substitution may be achieved by including a cryptographic unit within a computing device for binding multiple hardware devices and mutually authenticating the devices. The cryptographic unit includes a physically unclonable function ("PUF") circuit disposed in or on the hardware device, which generates a binding PUF value. The cryptographic unit uses the binding PUF value during an enrollment phase and subsequent authentication phases. During a subsequent authentication phase, the cryptographic unit uses the binding PUF values of the multiple hardware devices to generate a challenge to send to the other device, and to verify a challenge received from the other device to mutually authenticate the hardware devices.

  16. Transmission Line Circuit Alexander Glasser

    E-Print Network [OSTI]

    Anlage, Steven

    Chaos in a Transmission Line Circuit Alexander Glasser Marshal Miller With... Prof. Edward Ott Prof times become shorter, circuit connections behave more and more like transmission lines. Theoretical(t) - Transmission Line (Zo, T) #12;5 Cf/Cr 1000 Vf Capacitance Voltage Cf Cr Model for Nonlinear Capacitor

  17. RNA binding protein and binding site useful for expression of recombinant molecules

    DOE Patents [OSTI]

    Mayfield, Stephen (Cardiff, CA)

    2000-01-01

    The present invention relates to a gene expression system in eukaryotic and prokaryotic cells, preferably plant cells and intact plants. In particular, the invention relates to an expression system having a RB47 binding site upstream of a translation initiation site for regulation of translation mediated by binding of RB47 protein, a member of the poly(A) binding protein family. Regulation is further effected by RB60, a protein disulfide isomerase. The expression system is capable of functioning in the nuclear/cytoplasm of cells and in the chloroplast of plants. Translation regulation of a desired molecule is enhanced approximately 100 fold over that obtained without RB47 binding site activation.

  18. RNA binding protein and binding site useful for expression of recombinant molecules

    DOE Patents [OSTI]

    Mayfield, Stephen P.

    2006-10-17

    The present invention relates to a gene expression system in eukaryotic and prokaryotic cells, preferably plant cells and intact plants. In particular, the invention relates to an expression system having a RB47 binding site upstream of a translation initiation site for regulation of translation mediated by binding of RB47 protein, a member of the poly(A) binding protein family. Regulation is further effected by RB60, a protein disulfide isomerase. The expression system is capable of functioning in the nuclear/cytoplasm of cells and in the chloroplast of plants. Translation regulation of a desired molecule is enhanced approximately 100 fold over that obtained without RB47 binding site activation.

  19. Estrophilin immunoreactivity versus estrogen receptor binding activity in meningiomas: evidence for multiple estrogen binding sites

    SciTech Connect (OSTI)

    Lesch, K.P.; Schott, W.; Gross, S.

    1987-09-01

    The existence of estrogen receptors in human meningiomas has long been a controversial issue. This may be explained, in part, by apparent heterogeneity of estrogen binding sites in meningioma tissue. In this study, estrogen receptors were determined in 58 meningiomas with an enzyme immunoassay using monoclonal antibodies against human estrogen receptor protein (estrophilin) and with a sensitive radioligand binding assay using /sup 125/I-labeled estradiol (/sup 125/I-estradiol) as radioligand. Low levels of estrophilin immunoreactivity were found in tumors from 62% of patients, whereas radioligand binding activity was demonstrated in about 46% of the meningiomas examined. In eight (14%) tissue samples multiple binding sites for estradiol were observed. The immunoreactive binding sites correspond to the classical, high affinity estrogen receptors: the Kd for /sup 125/I-estradiol binding to the receptor was approximately 0.2 nM and the binding was specific for estrogens. The second, low affinity class of binding sites considerably influenced measurement of the classical receptor even at low ligand concentrations. The epidemiological and clinical data from patients with meningiomas, and the existence of specific estrogen receptors confirmed by immunochemical detection, may be important factors in a theory of oncogenesis.

  20. Plains and Eastern Clean Line Transmission Line: Federal Register...

    Energy Savers [EERE]

    Clean Line project. The project would include an overhead 600-kilovolt (kv) high voltage, direct current electric transmission system and associated facilities with the capacity to...

  1. Proposed Project: Plains & Eastern Clean Line Transmission Line...

    Energy Savers [EERE]

    & Eastern Clean Line project (the proposed project) would include an overhead +- 600 kV direct current electric transmission system and associated facilities with the capacity to...

  2. JAB1 regulates unphosphorylated STAT3 DNA-binding activity through protein–protein interaction in human colon cancer cells

    SciTech Connect (OSTI)

    Nishimoto, Arata, E-mail: anishimo@yamaguchi-u.ac.jp [Department of Surgery and Clinical Science, Yamaguchi University Graduate School of Medicine, 1-1-1 Minami-Kogushi, Ube, Yamaguchi 755-8505 (Japan)] [Department of Surgery and Clinical Science, Yamaguchi University Graduate School of Medicine, 1-1-1 Minami-Kogushi, Ube, Yamaguchi 755-8505 (Japan); Kugimiya, Naruji; Hosoyama, Toru; Enoki, Tadahiko [Department of Surgery and Clinical Science, Yamaguchi University Graduate School of Medicine, 1-1-1 Minami-Kogushi, Ube, Yamaguchi 755-8505 (Japan)] [Department of Surgery and Clinical Science, Yamaguchi University Graduate School of Medicine, 1-1-1 Minami-Kogushi, Ube, Yamaguchi 755-8505 (Japan); Li, Tao-Sheng [Department of Stem Cell Biology, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, Nagasaki 852-8523 (Japan)] [Department of Stem Cell Biology, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, Nagasaki 852-8523 (Japan); Hamano, Kimikazu [Department of Surgery and Clinical Science, Yamaguchi University Graduate School of Medicine, 1-1-1 Minami-Kogushi, Ube, Yamaguchi 755-8505 (Japan)] [Department of Surgery and Clinical Science, Yamaguchi University Graduate School of Medicine, 1-1-1 Minami-Kogushi, Ube, Yamaguchi 755-8505 (Japan)

    2013-08-30

    Highlights: •JAB1 interacted with unphosphorylated STAT3 in the nucleus. •JAB1 knockdown tended to increase nuclear STAT3 expression. •JAB1 knockdown significantly decreased unphosphorylated STAT3 DNA-binding activity. •JAB1 knockdown significantly decreased MDR1, NANOG, and VEGF expressions. •Nuclear JAB1, but not nuclear STAT3, correlated with STAT3 DNA-binding activity. -- Abstract: Recent studies have revealed that unphosphorylated STAT3 forms a dimer, translocates to the nucleus, binds to the STAT3 binding site, and activates the transcription of STAT3 target genes, thereby playing an important role in oncogenesis in addition to phosphorylated STAT3. Among signaling steps of unphosphorylated STAT3, nuclear translocation and target DNA-binding are the critical steps for its activation. Therefore, elucidating the regulatory mechanism of these signaling steps of unphosphorylated STAT3 is a potential step in the discovery of a novel cancer drug. However, the mechanism of unphosphorylated STAT3 binding to the promoter of target genes remains unclear. In this study, we focused on Jun activation domain-binding protein 1 (JAB1) as a candidate protein that regulates unphosphorylated STAT3 DNA-binding activity. Initially, we observed that both unphosphorylated STAT3 and JAB1 existed in the nucleus of human colon cancer cell line COLO205 at the basal state (no cytokine stimulation). On the other hand, phosphorylated STAT3 did not exist in the nucleus of COLO205 cells at the basal state. Immunoprecipitation using nuclear extract of COLO205 cells revealed that JAB1 interacted with unphosphorylated STAT3. To investigate the effect of JAB1 on unphosphorylated STAT3 activity, RNAi studies were performed. Although JAB1 knockdown tended to increase nuclear STAT3 expression, it significantly decreased unphosphorylated STAT3 DNA-binding activity. Subsequently, JAB1 knockdown significantly decreased the expression levels of MDR1, NANOG, and VEGF, which are STAT3 target genes. Furthermore, the expression level of nuclear JAB1, but not nuclear STAT3, correlated with unphosphorylated STAT3 DNA-binding activity between COLO205 and LoVo cells. Taken together, these results suggest that nuclear JAB1 positively regulates unphosphorylated STAT3 DNA-binding activity through protein–protein interaction in human colon cancer cell line COLO205.

  3. Bipolar pulse forming line

    DOE Patents [OSTI]

    Rhodes, Mark A. (Pleasanton, CA)

    2008-10-21

    A bipolar pulse forming transmission line module for linear induction accelerators having first, second, third, fourth, and fifth planar conductors which form an interleaved stack with dielectric layers between the conductors. Each conductor has a first end, and a second end adjacent an acceleration axis. The first and second planar conductors are connected to each other at the second ends, the fourth and fifth planar conductors are connected to each other at the second ends, and the first and fifth planar conductors are connected to each other at the first ends via a shorting plate adjacent the first ends. The third planar conductor is electrically connectable to a high voltage source, and an internal switch functions to short a high voltage from the first end of the third planar conductor to the first end of the fourth planar conductor to produce a bipolar pulse at the acceleration axis with a zero net time integral. Improved access to the switch is enabled by an aperture through the shorting plate and the proximity of the aperture to the switch.

  4. EC Transmission Line Materials

    SciTech Connect (OSTI)

    Bigelow, Tim S

    2012-05-01

    The purpose of this document is to identify materials acceptable for use in the US ITER Project Office (USIPO)-supplied components for the ITER Electron cyclotron Heating and Current Drive (ECH&CD) transmission lines (TL), PBS-52. The source of material property information for design analysis shall be either the applicable structural code or the ITER Material Properties Handbook. In the case of conflict, the ITER Material Properties Handbook shall take precedence. Materials selection, and use, shall follow the guidelines established in the Materials Assessment Report (MAR). Materials exposed to vacuum shall conform to the ITER Vacuum Handbook. [Ref. 2] Commercial materials shall conform to the applicable standard (e.g., ASTM, JIS, DIN) for the definition of their grade, physical, chemical and electrical properties and related testing. All materials for which a suitable certification from the supplier is not available shall be tested to determine the relevant properties, as part of the procurement. A complete traceability of all the materials including welding materials shall be provided. Halogenated materials (example: insulating materials) shall be forbidden in areas served by the detritiation systems. Exceptions must be approved by the Tritium System and Safety Section Responsible Officers.

  5. Emergency pipe line repair connects subsea pipe lines

    SciTech Connect (OSTI)

    Lerique, M.P.; Thiberge, P. ); Wright, N. )

    1990-11-01

    Emergency repair of any subsea line pipe must form a high-integrity, metal-to-metal seal. This paper presents a remote, diverless repair system that utilizes master flanges, a connector and a spool piece to repair line pipe in deep offshore waters.

  6. Localized states of a semi-infinite zigzag graphene sheet with impurity lines

    SciTech Connect (OSTI)

    Cunha, A. M. C.; Ahmed, Maher Z.; Cottam, M. G.; Filho, R. N. Costa

    2014-07-07

    The localized states of a semi-infinite zigzag graphene sheet are studied using a tight-binding model that allows for the inclusion of either one or two lines of impurities. These impurity lines of atoms are placed in rows labeled as n (n=1, 2, 3, …), where n=1 is the free edge. The localized defect modes associated with these impurities are studied analytically and numerically within a tridiagonal matrix formalism. For one impurity line, the modes are analyzed according to the position of that line on the sheet, whereas the modes for two impurities are studied also according to their separation and their positions relative to the edge. When an impurity line is located at the edge (n=1), it is found that the edge states are modified. When the impurities are positioned away from an edge (n>1), additional localized modes are found to occur that may be relatively flat in their dispersion.

  7. Binding Energies in Nonrelativistic Field Theories

    E-Print Network [OSTI]

    Andreas S. Kronfeld

    1996-08-26

    Relativistic corrections communicate the binding energy of a bound state to its kinetic mass. This mechanism is reviewed and used to explain anomalous results of Collins, Edwards, Heller, and Sloan (hep-lat/9512026), which compared rest and kinetic masses of heavy-light mesons and quarkonia.

  8. Nucleic acids encoding a cellulose binding domain

    DOE Patents [OSTI]

    Shoseyov, O.; Shpiegl, I.; Goldstein, M.A.; Doi, R.H.

    1996-03-05

    A cellulose binding domain (CBD) having a high affinity for crystalline cellulose and chitin is disclosed, along with methods for the molecular cloning and recombinant production. Fusion products comprising the CBD and a second protein are likewise described. A wide range of applications are contemplated for both the CBD and the fusion products, including drug delivery, affinity separations, and diagnostic techniques. 15 figs.

  9. BOUND SYSTEMS 4.A Binding Energy

    E-Print Network [OSTI]

    Boal, David

    CHAPTER 4 BOUND SYSTEMS 4.A Binding Energy The mass-energy equation (3.2) is valid for a single linear momentum and its energy. While we introduced the equation in order to describe the energy is measurable. The implication of (4.1) taken with (3.2) is that the mass energy of a bound state is less than

  10. Nucleic acids encoding a cellulose binding domain

    DOE Patents [OSTI]

    Shoseyov, Oded (Karmey Yosef, IL); Shpiegl, Itai (Rehovot, IL); Goldstein, Marc A. (Davis, CA); Doi, Roy H. (Davis, CA)

    1996-01-01

    A cellulose binding domain (CBD) having a high affinity for crystalline cellulose and chitin is disclosed, along with methods for the molecular cloning and recombinant production thereof. Fusion products comprising the CBD and a second protein are likewise described. A wide range of applications are contemplated for both the CBD and the fusion products, including drug delivery, affinity separations, and diagnostic techniques.

  11. Structural basis of CX-4945 binding to human protein kinase CK2

    SciTech Connect (OSTI)

    Ferguson, Andrew D.; Sheth, Payal R.; Basso, Andrea D.; Paliwal, Sunil; Gray, Kimberly; Fischmann, Thierry O.; Le, Hung V. (Merck)

    2012-02-07

    Protein kinase CK2 (CK2), a constitutively active serine/threonine kinase, is involved in a variety of roles essential to the maintenance of cellular homeostasis. Elevated levels of CK2 expression results in the dysregulation of key signaling pathways that regulate transcription, and has been implicated in cancer. The adenosine-5'-triphosphate-competitive inhibitor CX-4945 has been reported to show broad spectrum anti-proliferative activity in multiple cancer cell lines. Although the enzymatic IC{sub 50} of CX-4945 has been reported, the thermodynamics and structural basis of binding to CK2{alpha} remained elusive. Presented here are the crystal structures of human CK2{alpha} in complex with CX-4945 and adenylyl phosphoramidate at 2.7 and 1.3 {angstrom}, respectively. Biophysical analysis of CX-4945 binding is also described. This data provides the structural rationale for the design of more potent inhibitors against this emerging cancer target.

  12. Cnidocyte discharge is regulated by light and opsin-mediated phototransduction

    E-Print Network [OSTI]

    Plachetzki, David C; Fong, Caitlin R; Oakley, Todd H

    2012-01-01

    were captured using gelatin-coated lengths of monofilamentbetween dashed lines in (A) = gelatin matrix; asterisk =dipped twice in 20% gelatin (Knox) that had been dissolved

  13. Primary and Secondary Three Dimensional Microbatteries

    E-Print Network [OSTI]

    Cirigliano, Nicolas

    2013-01-01

    areal capacity (left) and energy density (right) for variouscapacity (left) and energy density (right). Dashed lines areareal capacity (left) and energy density (right) for various

  14. Pulse shaping with transmission lines

    DOE Patents [OSTI]

    Wilcox, Russell B. (Oakland, CA)

    1987-01-01

    A method and apparatus for forming shaped voltage pulses uses passive reflection from a transmission line with nonuniform impedance. The impedance of the reflecting line varies with length in accordance with the desired pulse shape. A high voltage input pulse is transmitted to the reflecting line. A reflected pulse is produced having the desired shape and is transmitted by pulse removal means to a load. Light activated photoconductive switches made of silicon can be utilized. The pulse shaper can be used to drive a Pockels cell to produce shaped optical pulses.

  15. Electrical transmission line diametrical retainer

    DOE Patents [OSTI]

    Hall, David R. (Provo, UT); Hall, Jr., H. Tracy (Provo, UT); Pixton, David (Lehi, UT); Dahlgren, Scott (Provo, UT); Sneddon, Cameron (Provo, UT); Briscoe, Michael (Lehi, UT); Fox, Joe (Spanish Fork, UT)

    2004-12-14

    The invention is a mechanism for retaining an electrical transmission line. In one embodiment of the invention it is a system for retaining an electrical transmission line within down hole components. In accordance with one aspect of the invention, the system includes a plurality of downhole components, such as sections of pipe in a drill string. The system also includes a coaxial cable running between the first and second end of a drill pipe, the coaxial cable having a conductive tube and a conductive core within it. The invention allows the electrical transmission line to with stand the tension and compression of drill pipe during routine drilling cycles.

  16. Study of fragmentation using clusterization algorithm with realistic binding energies

    E-Print Network [OSTI]

    Yogesh K. Vermani; Jatinder K. Dhawan; Supriya Goyal; Rajeev K. Puri; J. Aichelin

    2009-12-28

    We here study fragmentation using \\emph{simulated annealing clusterization algorithm} (SACA) with binding energy at a microscopic level. In an earlier version, a constant binding energy (4 MeV/nucleon) was used. We improve this binding energy criterion by calculating the binding energy of different clusters using modified Bethe-Weizs\\"{a}cker mass (BWM) formula. We also compare our calculations with experimental data of ALADiN group. Nearly no effect is visible of this modification.

  17. User Experiment Time-Line

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    User Experiment Time-Line Event Target Call for proposal 2 months before proposals are due PAC Proposals Due 7 weeks before PAC meeting TAC Report for PAC Proposals (includes...

  18. The beauty of laser lines

    E-Print Network [OSTI]

    Kim, Sue-Mie

    1993-01-01

    A line, the simplest way to express an artist's feelings or interpretation of an object, has its own emotions that an artist can employ for her purpose. Laser light, the most self-concentrated, self-sustained and directed, ...

  19. Predicting Metal-Binding Sites from Protein Sequence

    E-Print Network [OSTI]

    Passerini, Andrea

    Predicting Metal-Binding Sites from Protein Sequence Andrea Passerini, Marco Lippi, and Paolo algorithmic ideas based on structured- output learning for determining transition-metal-binding sites coordinate more than one metal ion, we prove that metal binding has the algebraic structure of a matroid

  20. Fin-line horn antenna

    DOE Patents [OSTI]

    Reindel, John (San Diego, CA)

    1990-01-01

    A fin line circuit card containing a fin line slot feeds a dipole antenna ich extends a quarterwave outside the waveguide and provides an energy beam focal point at or near the open end of the waveguide. The dipole antenna thus maintains a wide and nearly constant beamwidth, low VSWR and a circular symmetric radiation pattern for use in electronic warfare direction finding and surveillance applications.

  1. ORNL/UTK team maps the nuclear landscape The Chart of Nuclides is bounded by drip lines, where

    E-Print Network [OSTI]

    ORNL/UTK team maps the nuclear landscape The Chart of Nuclides is bounded by drip lines, where nuclear binding ends. The placement of these limits of nuclear existence, usually estimated from? The nuclear density functional theory, coupled with state-of-the-art computational tools, was used to estimate

  2. Dual chain synthetic heparin-binding growth factor analogs

    DOE Patents [OSTI]

    Zamora, Paul O. (Gaithersburg, MD); Pena, Louis A. (Poquott, NY); Lin, Xinhua (Plainview, NY)

    2012-04-24

    The invention provides synthetic heparin-binding growth factor analogs having two peptide chains each branched from a branch moiety, such as trifunctional amino acid residues, the branch moieties separated by a first linker of from 3 to about 20 backbone atoms, which peptide chains bind a heparin-binding growth factor receptor and are covalently bound to a non-signaling peptide that includes a heparin-binding domain, preferably by a second linker, which may be a hydrophobic second linker. The synthetic heparin-binding growth factor analogs are useful as pharmaceutical agents, soluble biologics or as surface coatings for medical devices.

  3. Dual chain synthetic heparin-binding growth factor analogs

    DOE Patents [OSTI]

    Zamora, Paul O. (Gaithersburg, MD); Pena, Louis A. (Poquott, NY); Lin, Xinhua (Plainview, NY)

    2009-10-06

    The invention provides synthetic heparin-binding growth factor analogs having two peptide chains each branched from a branch moiety, such as trifunctional amino acid residues, the branch moieties separated by a first linker of from 3 to about 20 backbone atoms, which peptide chains bind a heparin-binding growth factor receptor and are covalently bound to a non-signaling peptide that includes a heparin-binding domain, preferably by a second linker, which may be a hydrophobic second linker. The synthetic heparin-binding growth factor analogs are useful as pharmaceutical agents, soluble biologics or as surface coatings for medical devices.

  4. Multiple gamma lines from semi-annihilation

    E-Print Network [OSTI]

    D'Eramo, Francesco

    Hints in the Fermi data for a 130 GeV gamma line from the galactic center have ignited interest in potential gamma line signatures of dark matter. Explanations of this line based on dark matter annihilation face a parametric ...

  5. The Pulse Line Ion Accelerator Concept

    E-Print Network [OSTI]

    Briggs, Richard J.

    2006-01-01

    field model of the pulse- line accelerator; relationship to3, 2006 LBNL-59492 The pulse line ion accelerator conceptCalifornia, 94507 The Pulse Line Ion Accelerator concept was

  6. The COMPTEL instrumental line background

    E-Print Network [OSTI]

    G. Weidenspointner; M. Varendorff; U. Oberlack; D. Morris; S. Plueschke; R. Diehl; S. C. Kappadath; M. McConnell; J. Ryan; V. Schoenfelder; H. Steinle

    2000-12-14

    The instrumental line background of the Compton telescope COMPTEL onboard the Compton Gamma-Ray Observatory is due to the activation and/or decay of many isotopes. The major components of this background can be attributed to eight individual isotopes, namely 2D, 22Na, 24Na, 28Al, 40K, 52Mn, 57Ni, and 208Tl. The identification of instrumental lines with specific isotopes is based on the line energies as well as on the variation of the event rate with time, cosmic-ray intensity, and deposited radiation dose during passages through the South-Atlantic Anomaly. The characteristic variation of the event rate due to a specific isotope depends on its life-time, orbital parameters such as the altitude of the satellite above Earth, and the solar cycle. A detailed understanding of the background contributions from instrumental lines is crucial at MeV energies for measuring the cosmic diffuse gamma-ray background and for observing gamma-ray line emission in the interstellar medium or from supernovae and their remnants. Procedures to determine the event rate from each background isotope are described, and their average activity in spacecraft materials over the first seven years of the mission is estimated.

  7. Grand Coulee Transmission Line Replacement Project

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    & Reliability Projects Expand Projects Skip navigation links Line Projects Big Eddy-Knight Central Ferry Lower Monumental Grand Coulee Transmission Line Replacement...

  8. Transmission Line MTF: Magnetized Target Fusion

    E-Print Network [OSTI]

    Transmission Line MTF: Magnetized Target Fusion Initial target: preheated & magnetized Subsequent for the FRC. Abstract Block Diagram theta coil transmission line Bias cap. bank maincapacitor inductor PI cap

  9. What can emission lines tell us?

    E-Print Network [OSTI]

    G. Stasinska

    2007-04-03

    1 Generalities 2 Empirical diagnostics based on emission lines 3 Photoionization modelling 4 Pending questions 5 Appendix: Lists of useful lines and how to deal with them

  10. Polynucleotides encoding TRF1 binding proteins

    DOE Patents [OSTI]

    Campisi, Judith (Berkeley, CA); Kim, Sahn-Ho (Albany, CA)

    2002-01-01

    The present invention provides a novel telomere associated protein (Trf1-interacting nuclear protein 2 "Tin2") that hinders the binding of Trf1 to its specific telomere repeat sequence and mediates the formation of a Tin2-Trf1-telomeric DNA complex that limits telomerase access to the telomere. Also included are the corresponding nucleic acids that encode the Tin2 of the present invention, as well as mutants of Tin2. Methods of making, purifying and using Tin2 of the present invention are described. In addition, drug screening assays to identify drugs that mimic and/or complement the effect of Tin2 are presented.

  11. Conformational Variability of Organophosphorus Hydrolase upon Soman and Paraoxon Binding

    SciTech Connect (OSTI)

    Gomes, Diego Eb; Lins, Roberto D.; Pascutti, Pedro G.; Lei, Chenghong; Soares, Thereza A.

    2011-12-31

    The bacterial enzyme organophosphorus hydrolase (OPH) exhibits both catalytic and substrate promiscuity. It hydrolyzes bonds in a variety of phosphotriester (P-O), phosphonothioate (P-S), phosphofluoridate (P-F) and phosphonocyanate (F-CN) compounds. However, its catalytic efficiency varies markedly for different substrates, limiting the broad-range application of OPH as catalyst in the bioremediation of pesticides and chemical war agents. In the present study, pK{sub a} calculations and multiple explicit-solvent molecular dynamics (MD) simulations were performed to characterize and contrast the structural dynamics of OPH bound to two substrates hydrolyzed with very distinct catalytic efficiencies: the nerve agent soman (O-pinacolyl-methyl-phosphonofluoridate) and the pesticide paraoxon (diethyl p-nitrophenyl phosphate). pK{sub a} calculations for the substrate-bound and unbound enzyme showed a significant pK{sub a} shift from standard values ({Delta}pK{sub a} = {+-} 3 units) for residues 254His and 275Arg. MD simulations of the doubly protonated 254His revealed a dynamic hydrogen bond network connecting the catalytic residue 301Asp via 254His to 232Asp, 233Asp, 275Arg and 235Asp, and is consistent with a previously postulated proton relay mechanism to ferry protons away from the active site with substrates that do not require activation of the leaving group. Hydrogen bonds between 301Asp and 254His were persistent in the OPH-paraoxon complex but not in the OPH-soman one, suggesting a potential role for such interaction in the more efficient hydrolysis of paraoxon over soman by OPH. These results are in line with previous mutational studies of residue 254His, which led to an increase of the catalytic efficiency of OPH over soman yet decreased its efficiency for paraoxon. In addition, comparative analysis of the molecular trajectories for OPH bound to soman and paraoxon suggests that binding of the latter facilitates the conformational transition of OPH from the open to the closed substate promoting a tighter binding of paraoxon.

  12. Connotation Lexicon: A Dash of Sentiment Beneath the Surface Meaning

    E-Print Network [OSTI]

    Anderson, Richard

    of sentiment beyond denotative or surface meaning of text. For instance, consider the following: Geothermal toward "geothermal". In order to sense the subtle overtone of sentiments, one needs to know that the word "emissions" has generally negative connota- tion, which geothermal reduces. In fact, depend- ing

  13. Dash for Bikes, Walk for Trikes | National Nuclear Security Administration

    National Nuclear Security Administration (NNSA)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of NaturalDukeWakefield Municipal Gas &SCE-SessionsSouthReporteeo | National/%2A en| National Nuclear

  14. Coiled transmission line pulse generators

    DOE Patents [OSTI]

    McDonald, Kenneth Fox (Columbia, MO)

    2010-11-09

    Methods and apparatus are provided for fabricating and constructing solid dielectric "Coiled Transmission Line" pulse generators in radial or axial coiled geometries. The pour and cure fabrication process enables a wide variety of geometries and form factors. The volume between the conductors is filled with liquid blends of monomers, polymers, oligomers, and/or cross-linkers and dielectric powders; and then cured to form high field strength and high dielectric constant solid dielectric transmission lines that intrinsically produce ideal rectangular high voltage pulses when charged and switched into matched impedance loads. Voltage levels may be increased by Marx and/or Blumlein principles incorporating spark gap or, preferentially, solid state switches (such as optically triggered thyristors) which produce reliable, high repetition rate operation. Moreover, these Marxed pulse generators can be DC charged and do not require additional pulse forming circuitry, pulse forming lines, transformers, or an a high voltage spark gap output switch. The apparatus accommodates a wide range of voltages, impedances, pulse durations, pulse repetition rates, and duty cycles. The resulting mobile or flight platform friendly cylindrical geometric configuration is much more compact, light-weight, and robust than conventional linear geometries, or pulse generators constructed from conventional components. Installing additional circuitry may accommodate optional pulse shape improvements. The Coiled Transmission Lines can also be connected in parallel to decrease the impedance, or in series to increase the pulse length.

  15. Hardware device to physical structure binding and authentication

    SciTech Connect (OSTI)

    Hamlet, Jason R.; Stein, David J.; Bauer, Todd M.

    2013-08-20

    Detection and deterrence of device tampering and subversion may be achieved by including a cryptographic fingerprint unit within a hardware device for authenticating a binding of the hardware device and a physical structure. The cryptographic fingerprint unit includes an internal physically unclonable function ("PUF") circuit disposed in or on the hardware device, which generate an internal PUF value. Binding logic is coupled to receive the internal PUF value, as well as an external PUF value associated with the physical structure, and generates a binding PUF value, which represents the binding of the hardware device and the physical structure. The cryptographic fingerprint unit also includes a cryptographic unit that uses the binding PUF value to allow a challenger to authenticate the binding.

  16. Crown Ethers Flatten in Graphene for Strong, Specific Binding...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    2011 2010 News Home | ORNL | News | Features | 2014 SHARE Crown Ethers Flatten in Graphene for Strong, Specific Binding ORNL discovery holds potential for separations, sensors,...

  17. Neptunium Binding Kinetics with Arsenazo(III)

    SciTech Connect (OSTI)

    Leigh R. Martin; Aaron T. Johnson; Stephen P. Mezyk

    2014-08-01

    This document has been prepared to meet FCR&D level 2 milestone M2FT-14IN0304021, “Report on the results of actinide binding kinetics with aqueous phase complexants” This work was carried out under the auspices of the Thermodynamics and Kinetics of Advanced Separations Systems FCR&D work package. The report details kinetics experiments that were performed to measure rates of aqueous phase complexation for pentavalent neptunium with the chromotropic dye Arsenazo III (AAIII). The studies performed were designed to determine how pH, ionic strength and AAIII concentration may affect the rate of the reaction. A brief comparison with hexavalent neptunium is also made. It was identified that as pH was increased the rate of reaction also increased, however increasing the ionic strength and concentration of AAIII had the opposite effect. Interestingly, the rate of reaction of Np(VI) with AAIII was found to be slower than that of the Np(V) reaction.

  18. In-line thermoelectric module

    DOE Patents [OSTI]

    Pento, Robert (Algonquin, IL); Marks, James E. (Glenville, NY); Staffanson, Clifford D. (S. Glens Falls, NY)

    2000-01-01

    A thermoelectric module with a plurality of electricity generating units each having a first end and a second end, the units being arranged first end to second end along an in-line axis. Each unit includes first and second elements each made of a thermoelectric material, an electrically conductive hot member arranged to heat one side of the first element, and an electrically conductive cold member arranged to cool another side of the first element and to cool one side of the second element. The hot member, the first element, the cold member and the second element are supported in a fixture, are electrically connected respectively to provide an electricity generating unit, and are arranged respectively in positions along the in-line axis. The individual components of each generating unit and the respective generating units are clamped in their in-line positions by a loading bolt at one end of the fixture and a stop wall at the other end of the fixture. The hot members may have a T-shape and the cold members an hourglass shape to facilitate heat transfer. The direction of heat transfer through the hot members may be perpendicular to the direction of heat transfer through the cold members, and both of these heat transfer directions may be perpendicular to the direction of current flow through the module.

  19. Design of Bcl-2 and Bcl-xL Inhibitors with Subnanomolar Binding Affinities Based upon a New Scaffold

    SciTech Connect (OSTI)

    Zhou, Haibin; Chen, Jianfang; Meagher, Jennifer L.; Yang, Chao-Yie; Aguilar, Angelo; Liu, Liu; Bai, Longchuan; Cong, Xin; Cai, Qian; Fang, Xueliang; Stuckey, Jeanne A.; Wang, Shaomeng

    2014-10-02

    Employing a structure-based strategy, we have designed a new class of potent small-molecule inhibitors of the anti-apoptotic proteins Bcl-2 and Bcl-xL. An initial lead compound with a new scaffold was designed based upon the crystal structure of Bcl-xL and U.S. Food and Drug Administration (FDA) approved drugs and was found to have an affinity of 100 {micro}M for both Bcl-2 and Bcl-xL. Linking this weak lead to another weak-affinity fragment derived from Abbott's ABT-737 led to an improvement of the binding affinity by a factor of >10,000. Further optimization ultimately yielded compounds with subnanomolar binding affinities for both Bcl-2 and Bcl-xL and potent cellular activity. The best compound (21) binds to Bcl-xL and Bcl-2 with K{sub i} < 1 nM, inhibits cell growth in the H146 and H1417 small-cell lung cancer cell lines with IC{sub 50} values of 60-90 nM, and induces robust cell death in the H146 cancer cell line at 30-100 nM.

  20. Borgers et al. Approximate, not perfect synchrony maximizes the

    E-Print Network [OSTI]

    Börgers, Christoph

    @tufts.edu 1 Department of Mathematics, Tufts University, Medford, MA 02155, USA Full list of author and inhibitory neurons (E- and I-cells); see Section 2.6 for the complete details. There are 200 E-cells (above the dashed line in the figure) and 50 I-cells (below the dashed line). The E-cells receive strong external

  1. Development of A Bayesian Geostatistical Data Assimilation Method and Application to the Hanford 300 Area

    E-Print Network [OSTI]

    Murakami, Haruko

    2010-01-01

    the black line is the mean BTC, the dash lines are 95% CIread line is the observed BTC. . . 4.6 95% CI and mean of M0valued metrics from each BTC to evaluate the improvement. We

  2. Microsoft Word - Defense Science Quarterly 05-08.doc

    National Nuclear Security Administration (NNSA)

    flyer plates (blue and green solid lines) as well as predicted shock response (blue and green dashed lines). Note: Red and black lines show a control experiment conducted with a...

  3. Chemomechanical mapping of ligandreceptor binding kinetics on cells

    E-Print Network [OSTI]

    Van Vliet, Krystyn J.

    Chemomechanical mapping of ligand­receptor binding kinetics on cells Sunyoung Lee, Jelena Mandic, Providence, RI, April 23, 2007 (received for review January 2, 2007) The binding kinetics between cell activity. Modeling and prediction of receptor-mediated cell func- tions are facilitated by measurement

  4. Water at biomolecular binding interfaces Zheng Li and Themis Lazaridis*

    E-Print Network [OSTI]

    Lazaridis, Themis

    Water at biomolecular binding interfaces Zheng Li and Themis Lazaridis* Received 29th August 2006.1039/b612449f Water molecules are often found at the binding interface of biomolecular complexes Waals interactions. Recent studies have demonstrated the importance of taking such water molecules

  5. Hydrogen Bonding Penalty upon Ligand Binding Hongtao Zhao, Danzhi Huang*

    E-Print Network [OSTI]

    Caflisch, Amedeo

    Hydrogen Bonding Penalty upon Ligand Binding Hongtao Zhao, Danzhi Huang* Department of Biochemistry, University of Zurich, Zurich, Switzerland Abstract Ligand binding involves breakage of hydrogen bonds with water molecules and formation of new hydrogen bonds between protein and ligand. In this work, the change

  6. ORIGINAL ARTICLE Analysis of RUNX1 binding site and RAPTOR

    E-Print Network [OSTI]

    Abecasis, Goncalo

    ORIGINAL ARTICLE Analysis of RUNX1 binding site and RAPTOR polymorphisms in psoriasis: no evidence psoriasis and single nucleotide polymorphisms (SNPs) mapping to distal chromosome 17q, including a disease for the previously identified RUNX1 binding site or for the RAPTOR locus as genetic determinants of psoriasis

  7. Performance-Guided Character Bind Pose for Deformations 

    E-Print Network [OSTI]

    Pena, Benito

    2011-08-08

    Current production methods for creating a motion system for a deformable digital character model involve providing an underlying joint structure based o of a T-Pose, A-Pose or another arbitrary bind pose of the character. A bind pose is required...

  8. NUCLEAR FISSION AND FUSION 6.A Nuclear Binding Energies

    E-Print Network [OSTI]

    Boal, David

    CHAPTER 6 NUCLEAR FISSION AND FUSION 6.A Nuclear Binding Energies A nucleus is characterized emphasis on the nuclear charge, the mass number of a nucleus plays a large role in its binding energy, and is denoted by 7Li. Some further items from the nuclear lexicon: nuclei with the same Z and differing N

  9. NETL LINES OF DEMARCATION 09282012

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration wouldMass map shines light on771/6/14 Contact: Janet Lambert Reviewed: 3/5/14 Page 1 of 178LINES

  10. Capillary electrophoresis-fluorescence line narrowing system (CE-FLNS) for on-line structural characterization

    DOE Patents [OSTI]

    Jankowiak, Ryszard J. (Ames, IA); Small, Gerald J. (Ames, IA); Shields, Peter A. (Reading, MA)

    1999-04-27

    Capillary electrophoresis (CE) is interfaced with low temperature fluorescence line-narrowing (FLN) spectroscopy for on-line structural characterization of separated molecular analytes.

  11. Capillary electrophoresis-fluorescence line narrowing system (CE-FLNS) for on-line structural characterization

    DOE Patents [OSTI]

    Jankowiak, R.J.; Small, G.J.; Shields, P.A.

    1999-04-27

    Capillary electrophoresis (CE) is interfaced with low temperature fluorescence line-narrowing (FLN) spectroscopy for on-line structural characterization of separated molecular analytes. 21 figs.

  12. Coordination of Transmission Line Transfer Capabilities

    E-Print Network [OSTI]

    Coordination of Transmission Line Transfer Capabilities Final Project Report Power Systems since 1996 PSERC #12;Power Systems Engineering Research Center Coordination of Transmission Line Industry Representative Richard Goddard Portland General Electric Research Team Students Yuan Li Yonghong

  13. Nondestructive Testing of Rail Tunnel Linings 

    E-Print Network [OSTI]

    Williams, Nathan Douglas

    2014-11-14

    potential problems and then test those areas with slow, detailed methods. This would provide a more thorough investigation of the tunnel lining’s health. Infrared thermography (IRT), ground penetrating radar (GPR), and ultrasonic tomography (UST) techniques...

  14. Calcium Binding by the N-Terminal Cellulose-Binding Domain from Cellulomonas fimi -1,4-Glucanase CenC

    E-Print Network [OSTI]

    McIntosh, Lawrence P.

    Calcium Binding by the N-Terminal Cellulose-Binding Domain from Cellulomonas fimi -1,4-Glucanase, 1998 ABSTRACT: The interaction of the N-terminal cellulose-binding domain, CBDN1, from Cellulomonas-state stability. Enzymes that degrade cellulose often contain one or more domains that mediate binding

  15. World-line approach to Sign Problems

    E-Print Network [OSTI]

    Aarts, Gert

    World-line approach to Sign Problems Shailesh Chandrasekharan Duke University #12;Outline XY model + chemical potential world-line approach solving the silver blaze problem! Two component bosons + Abelian world-line approach solving the silver blaze problem! more flavors + solving sign problems

  16. Linear rotary optical delay lines Maksim Skorobogatiy*

    E-Print Network [OSTI]

    Skorobogatiy, Maksim

    Linear rotary optical delay lines Maksim Skorobogatiy* Department of Engineering Physics, École of analytical and semi-analytical solutions for the design of high-speed rotary optical delay lines that use of optical delay. Finally, two prototypes of rotary delay lines were fabricated using CNC machining

  17. Characterization of the Carbohydrate Binding Module 18 gene family in the amphibian pathogen Batrachochytrium dendrobatidis.

    E-Print Network [OSTI]

    Liu, Peng; Stajich, Jason E

    2015-01-01

    a! chitin9binding! module. ! Appl! Microbiol! Biotechnol. !Species9specific! chitin9binding! module! 18! expansion! in!of the Carbohydrate Binding Module 18 gene family in the

  18. Page 1 A dedicated binding mechanism for the visual control of movement

    E-Print Network [OSTI]

    Diedrichsen, Jörn

    Page 1 A dedicated binding mechanism for the visual control of movement results therefore suggest the existence of a dedicated visuo-motor binding mechanism visual target information · A dedicated visuo-motor binding mechanism links visual

  19. Structural and Histone Binding Ability Characterizations of Human PWWP Domains

    SciTech Connect (OSTI)

    Wu, Hong; Zeng, Hong; Lam, Robert; Tempel, Wolfram; Amaya, Maria F.; Xu, Chao; Dombrovski, Ludmila; Qiu, Wei; Wang, Yanming; Min, Jinrong (Toronto); (Penn)

    2013-09-25

    The PWWP domain was first identified as a structural motif of 100-130 amino acids in the WHSC1 protein and predicted to be a protein-protein interaction domain. It belongs to the Tudor domain 'Royal Family', which consists of Tudor, chromodomain, MBT and PWWP domains. While Tudor, chromodomain and MBT domains have long been known to bind methylated histones, PWWP was shown to exhibit histone binding ability only until recently. The PWWP domain has been shown to be a DNA binding domain, but sequence analysis and previous structural studies show that the PWWP domain exhibits significant similarity to other 'Royal Family' members, implying that the PWWP domain has the potential to bind histones. In order to further explore the function of the PWWP domain, we used the protein family approach to determine the crystal structures of the PWWP domains from seven different human proteins. Our fluorescence polarization binding studies show that PWWP domains have weak histone binding ability, which is also confirmed by our NMR titration experiments. Furthermore, we determined the crystal structures of the BRPF1 PWWP domain in complex with H3K36me3, and HDGF2 PWWP domain in complex with H3K79me3 and H4K20me3. PWWP proteins constitute a new family of methyl lysine histone binders. The PWWP domain consists of three motifs: a canonical {beta}-barrel core, an insertion motif between the second and third {beta}-strands and a C-terminal {alpha}-helix bundle. Both the canonical {beta}-barrel core and the insertion motif are directly involved in histone binding. The PWWP domain has been previously shown to be a DNA binding domain. Therefore, the PWWP domain exhibits dual functions: binding both DNA and methyllysine histones.

  20. Mode propagation and attenuation in lined ducts

    E-Print Network [OSTI]

    BI, Wenping

    2014-01-01

    Optimal impedance for each mode is an important concept in an infinitely long duct lined with uniform absorption material. However it is not valid for finite length linings. This is because that the modes in lined ducts are not power-orthogonal; the total sound power is not equal to the sum of the sound power of each mode; cross-power terms may play important roles. In this paper, we study sound propagation and attenuation in an infinite rigid duct lined with a finite length of lining impedance. The lining impedance may be axial segments and circumferentially non-uniform. We propose two new physical quantities Kp and S to describe the self-overlap of the left eigenfunction and right eigenfunction of one mode and the normalized overlap between modes, respectively. The two new physical quantities describe totally the mode behaviors in lined ducts.

  1. Unsplit bipolar pulse forming line

    DOE Patents [OSTI]

    Rhodes, Mark A. (Pleasanton, CA)

    2011-05-24

    A bipolar pulse forming transmission line module and system for linear induction accelerators having first, second, third, and fourth planar conductors which form a sequentially arranged interleaved stack having opposing first and second ends, with dielectric layers between the conductors. The first and second planar conductors are connected to each other at the first end, and the first and fourth planar conductors are connected to each other at the second end via a shorting plate. The third planar conductor is electrically connectable to a high voltage source, and an internal switch functions to short at the first end a high voltage from the third planar conductor to the fourth planar conductor to produce a bipolar pulse at the acceleration axis with a zero net time integral. Improved access to the switch is enabled by an aperture through the shorting plate and the proximity of the aperture to the switch.

  2. Magnetically insulated transmission line oscillator

    DOE Patents [OSTI]

    Bacon, L.D.; Ballard, W.P.; Clark, M.C.; Marder, B.M.

    1987-05-19

    A magnetically insulated transmission line oscillator employs self-generated magnetic fields to generate microwave energy. An anode of the oscillator includes slow-wave structures which are formed of a plurality of thin conductive vanes defining cavities therebetween, and a gap is formed between the anode and a cathode of the oscillator. In response to a pulsed voltage applied to the anode and cathode, self-generated magnetic fields are produced in a cross-field orientation with respect to the orientation of the electric field between the anode and the cathode. The cross-field magnetic fields insulate the flow of electrons in the gap and confine the flow of electrons within the gap. 11 figs.

  3. Superconducting transmission line particle detector

    DOE Patents [OSTI]

    Gray, Kenneth E. (Naperville, IL)

    1989-01-01

    A microvertex particle detector for use in a high energy physic collider including a plurality of parallel superconducting thin film strips separated from a superconducting ground plane by an insulating layer to form a plurality of superconducting waveguides. The microvertex particle detector indicates passage of a charged subatomic particle by measuring a voltage pulse measured across a superconducting waveguide caused by the transition of the superconducting thin film strip from a superconducting to a non-superconducting state in response to the passage of a charged particle. A plurality of superconducting thin film strips in two orthogonal planes plus the slow electromagnetic wave propogating in a superconducting transmission line are used to resolve N.sup.2 ambiguity of charged particle events.

  4. Magnetically insulated transmission line oscillator

    DOE Patents [OSTI]

    Bacon, Larry D. (Albuquerque, NM); Ballard, William P. (Albuquerque, NM); Clark, M. Collins (Albuquerque, NM); Marder, Barry M. (Albuquerque, NM)

    1988-01-01

    A magnetically insulated transmission line oscillator employs self-generated magnetic fields to generate microwave energy. An anode of the oscillator includes slow-wave structures which are formed of a plurality of thin conductive vanes defining cavities therebetween, and a gap is formed between the anode and a cathode of the oscillator. In response to a pulsed voltage applied to the anode and cathode, self-generated magnetic fields arfe produced in a cross-field orientation with respect to the orientation of the electric field between the anode and the cathode. The cross-field magnetic fields insulate the flow of electrons in the gap and confine the flow of electrons within the gap.

  5. Gamma-Ray Burst Lines

    E-Print Network [OSTI]

    Michael S. Briggs

    1999-10-20

    The evidence for spectral features in gamma-ray bursts is summarized. As a guide for evaluating the evidence, the properties of gamma-ray detectors and the methods of analyzing gamma-ray spectra are reviewed. In the 1980's, observations indicated that absorption features below 100 keV were present in a large fraction of bright gamma-ray bursts. There were also reports of emission features around 400 keV. During the 1990's the situation has become much less clear. A small fraction of bursts observed with BATSE have statistically significant low-energy features, but the reality of the features is suspect because in several cases the data of the BATSE detectors appear to be inconsistent. Furthermore, most of the possible features appear in emission rather than the expected absorption. Analysis of data from other instruments has either not been finalized or has not detected lines.

  6. Microlensing induced absorption line variability

    E-Print Network [OSTI]

    Geraint F. Lewis; Rodrigo A. Ibata

    2002-12-02

    Gravitational microlensing has proven to be a powerful probe of both the structure at the heart of quasars and the mass function of compact objects in foreground lenses. This paper examines the potential of gravitational microlensing in probing the scale of structure in absorbing material within the lensing galaxy. We find that, in this high optical depth regime, significant variations in the equivalent width of absorption features can be induced, although the details of these are dependent upon the scale of structure of the absorbing material. The paper concludes with an examination of the absorption line variability observed in the gravitationally lensed quasar PKS1830-211, demonstrating how this may indicate the presence of small scale structure in the cold molecular gas present within the lensing galaxy.

  7. Superconducting transmission line particle detector

    DOE Patents [OSTI]

    Gray, K.E.

    1988-07-28

    A microvertex particle detector for use in a high energy physic collider including a plurality of parallel superconducting thin film strips separated from a superconducting ground plane by an insulating layer to form a plurality of superconducting waveguides. The microvertex particle detector indicates passage of a charged subatomic particle by measuring a voltage pulse measured across a superconducting waveguide caused by the transition of the superconducting thin film strip from a superconducting to a non- superconducting state in response to the passage of a charged particle. A plurality of superconducting thin film strips in two orthogonal planes plus the slow electromagnetic wave propagating in a superconducting transmission line are used to resolve N/sup 2/ ambiguity of charged particle events. 6 figs.

  8. Supplemental On-Line Materials Supplemental on line materials 1 of 12

    E-Print Network [OSTI]

    Bishop, James K.B.

    Supplemental On-Line Materials Supplemental on line materials 1 of 12 Robotic ObservationsX: Enhanced Carbon Biomass and Export at 55S Supplemental on line materials 2 of 12 Carbon Explorer / Patch to NZ B #12;Bishop et al. SOFeX: Enhanced Carbon Biomass and Export at 55S Supplemental on line

  9. X-ray Crystallographic Studies of Substrate Binding to Aristolochene Synthase Suggest a Metal Ion Binding Sequence for Catalysis

    SciTech Connect (OSTI)

    Shishova,E.; Yu, F.; Miller, D.; Faraldos, J.; Zhao, Y.; Coates, R.; Allemann, R.; Cane, D.; Christianson, D.

    2008-01-01

    The universal sesquiterpene precursor, farnesyl diphosphate (FPP), is cyclized in an Mg2+-dependent reaction catalyzed by the tetrameric aristolochene synthase from Aspergillus terreus to form the bicyclic hydrocarbon aristolochene and a pyrophosphate anion (PPi) coproduct. The 2.1- Angstroms resolution crystal structure determined from crystals soaked with FPP reveals the binding of intact FPP to monomers A-C, and the binding of PPi and Mg2+B to monomer D. The 1.89- Angstroms resolution structure of the complex with 2-fluorofarnesyl diphosphate (2F-FPP) reveals 2F-FPP binding to all subunits of the tetramer, with Mg2+Baccompanying the binding of this analogue only in monomer D. All monomers adopt open activesite conformations in these complexes, but slight structural changes in monomers C and D of each complex reflect the very initial stages of a conformational transition to the closed state. Finally, the 2.4- Angstroms resolution structure of the complex with 12,13-difluorofarnesyl diphosphate (DF-FPP) reveals the binding of intact DF-FPP to monomers A-C in the open conformation and the binding of PPi, Mg2+B, and Mg2+C to monomer D in a predominantly closed conformation. Taken together, these structures provide 12 independent 'snapshots' of substrate or product complexes that suggest a possible sequence for metal ion binding and conformational changes required for catalysis.

  10. Cellulose Binding Protein from the Parasitic Nematode Heterodera schachtii Interacts with Arabidopsis Pectin

    E-Print Network [OSTI]

    Hussey, Richard S.

    Cellulose Binding Protein from the Parasitic Nematode Heterodera schachtii Interacts Heterodera glycines also produces a cellulose binding protein (Hg CBP) secretory protein. To determine

  11. Post-transcriptional coordination by an RNA-binding protein

    E-Print Network [OSTI]

    Wolf, Joshua Jaeger

    2010-01-01

    RNA-binding proteins can regulate the stability, localization, and translation of their target mRNAs. Post-transcriptional regulation can orchestrate dynamic changes in gene expression, and can coordinate multiple cellular ...

  12. Original article Distribution of endogenous retinoids, retinoid binding

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    of specific retinoid-binding proteins was investigated; these are involved in vitamin A transport, metabolism is induced by a concentration gradient (high posteri- orly) of all-trans-RA along the anterior-p

  13. Metal binding proteins, recombinant host cells and methods

    DOE Patents [OSTI]

    Summers, Anne O.; Caguiat, Jonathan J.

    2004-06-15

    The present disclosure provides artificial heavy metal binding proteins termed chelons by the inventors. These chelons bind cadmium and/or mercuric ions with relatively high affinity. Also disclosed are coding sequences, recombinant DNA molecules and recombinant host cells comprising those recombinant DNA molecules for expression of the chelon proteins. In the recombinant host cells or transgenic plants, the chelons can be used to bind heavy metals taken up from contaminated soil, groundwater or irrigation water and to concentrate and sequester those ions. Recombinant enteric bacteria can be used within the gastrointestinal tracts of animals or humans exposed to toxic metal ions such as mercury and/or cadmium, where the chelon recombinantly expressed in chosen in accordance with the ion to be rededicated. Alternatively, the chelons can be immobilized to solid supports to bind and concentrate heavy metals from a contaminated aqueous medium including biological fluids.

  14. Linear Scaling of the Exciton Binding Energy versus the Band...

    Office of Scientific and Technical Information (OSTI)

    Linear Scaling of the Exciton Binding Energy versus the Band Gap of Two-Dimensional Materials This content will become publicly available on August 6, 2016 Prev Next Title:...

  15. Second-order susceptibility from a tight-binding Hamiltonian 

    E-Print Network [OSTI]

    Dumitrica, T.; Graves, JS; Allen, Roland E.

    1998-01-01

    Using a new formalism that modifies a tight-binding Hamiltonian to include interaction with a time-dependent electromagnetic field, we have obtained an analytical expression for the second-order susceptibility. This expression has been used...

  16. MCM ring hexamerization is a prerequisite for DNA-binding

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Froelich, Clifford A.; Nourse, Amanda; Enemark, Eric J.

    2015-09-13

    The hexameric Minichromosome Maintenance (MCM) protein complex forms a ring that unwinds DNA at the replication fork in eukaryotes and archaea. Our recent crystal structure of an archaeal MCM N-terminal domain bound to single-stranded DNA (ssDNA) revealed ssDNA associating across tight subunit interfaces but not at the loose interfaces, indicating that DNA-binding is governed not only by the DNA-binding residues of the subunits (MCM ssDNA-binding motif, MSSB) but also by the relative orientation of the subunits. We now extend these findings to show that DNA-binding by the MCM N-terminal domain of the archaeal organism Pyrococcus furiosus occurs specifically in themore »hexameric oligomeric form. We show that mutants defective for hexamerization are defective in binding ssDNA despite retaining all the residues observed to interact with ssDNA in the crystal structure. One mutation that exhibits severely defective hexamerization and ssDNA-binding is at a conserved phenylalanine that aligns with the mouse Mcm4(Chaos3) mutation associated with chromosomal instability, cancer, and decreased intersubunit association.« less

  17. NSLS-II Transport Line Progress

    SciTech Connect (OSTI)

    Fliller R. P.; Wahl, W.; Anderson, A.; Benish, B.; DeBoer, W.; Ganetis, G.; Heese, R.; Hseuh, H.-C.; Hu, J.-P.; Johanson, M.P.; Kosciuk, B.N.; Padrazo, D.; Roy, K.; Shaftan, T.; Singh, O.; Tuozzolo, J.; Wang, G.

    2012-05-20

    The National Synchrotron Light Source II (NSLS-II) is a state-of-the-art 3-GeV third generation light source currently under construction at Brookhaven National Laboratory. The NSLS-II injection system consists of a 200 MeV linac, a 3-GeV booster synchrotron and associated transfer lines. The first part of the Linac to Booster Transport (LBT) line has been installed for linac commissioning. This part includes all components necessary to commission the NSLS-II linac. The second part of this transport line is undergoing installation. Initial results of hardware commissioning will be discussed. The Booster to Storage Ring (BSR) transport line underwent a design review. The first part of the BSR transport line, consisting of all components necessary to commission the booster will be installed in 2012 for booster commissioning. We report on the final design of the BSR line along with the plan to commission the booster.

  18. Single transmission line data acquisition system

    DOE Patents [OSTI]

    Fasching, George E. (Morgantown, WV)

    1984-01-01

    A single transmission line interrogated multiple channel data acquisition system is provided in which a plurality of remote station/sensors monitor specific process variables and transmit measurement values over the single transmission line to a master station when addressed by the master station. Power for all remote stations (up to 980) is provided by driving the line with constant voltage supplied from the master station and automatically maintained independent of the number of remote stations directly connected to the line. The transmission line can be an RG-62 coaxial cable with lengths up to about 10,000 feet with branches up to 500 feet. The remote stations can be attached randomly along the line. The remote stations can be scanned at rates up to 980 channels/second.

  19. Wilson Line Integrals in the Unparticle Action

    E-Print Network [OSTI]

    A. Lewis Licht

    2008-05-25

    We consider the unparticle action that is made gauge invariant by inclusion of an open Wilson line factor. In deriving vertexes from such an action it has been customary to use a form of differentiating the Wilson line originally proposed by Mandelstam. Using a simple example, we show that the Mandelstam derivative is mathematically inconsistent. We show that there are two ways to define differentiation of the Wilson line. The mathematically consistent method is to differentiate the explicit dependence of the line on the endpoint. The other method is a functional derivative and corresponds in a limiting case to the Mandelstam derivative. We also show that the only path that can be used in the Wilson line integral that leaves the unparticle action both Poincare and scale invariant is the straight line.

  20. Gamma-Ray Line Observations with RHESSI

    E-Print Network [OSTI]

    David M. Smith

    2004-04-30

    The Reuven Ramaty High Energy Solar Spectroscopic Imager (RHESSI) has been observing gamma-ray lines from the Sun and the Galaxy since its launch in February 2002. Here I summarize the status of RHESSI observations of solar lines (nuclear de-excitation, neutron capture, and positron annihilation), the lines of $^{26}$Al and $^{60}$Fe from the inner Galaxy, and the search for positron annihilation in novae.

  1. Northern Pass Transmission Line Project Environmental Impact...

    Broader source: Energy.gov (indexed) [DOE]

    the location of the September 26 public scoping meeting for the Northern Pass Transmission Line Project to Colebrook Elementary School, 27 Dumont Street, Colebrook, NH. On...

  2. Transmission Line Security Monitor: Final Report

    SciTech Connect (OSTI)

    John Svoboda

    2011-04-01

    The Electric Power Transmission Line Security Monitor System Operational Test is a project funded by the Technical Support Working Group (TSWG). TSWG operates under the Combating Terrorism Technical Support Office that functions under the Department of Defense. The Transmission Line Security Monitor System is based on technology developed by Idaho National Laboratory. The technology provides a means for real-time monitoring of physical threats and/or damage to electrical transmission line towers and conductors as well as providing operational parameters to transmission line operators to optimize transmission line operation. The end use is for monitoring long stretches of transmission lines that deliver electrical power from remote generating stations to cities and industry. These transmission lines are generally located in remote transmission line corridors where security infrastructure may not exist. Security and operational sensors in the sensor platform on the conductors take power from the transmission line and relay security and operational information to operations personnel hundreds of miles away without relying on existing infrastructure. Initiated on May 25, 2007, this project resulted in pre-production units tested in realistic operational environments during 2010. A technology licensee, Lindsey Manufacturing of Azusa California, is assisting in design, testing, and ultimately production. The platform was originally designed for a security monitoring mission, but it has been enhanced to include important operational features desired by electrical utilities.

  3. Detonator comprising a nonlinear transmission line

    DOE Patents [OSTI]

    Elizondo-Decanini, Juan M

    2014-12-30

    Detonators are described herein. In a general embodiment, the detonator includes a nonlinear transmission line that has a variable capacitance. Capacitance of the nonlinear transmission line is a function of voltage on the nonlinear transmission line. The nonlinear transmission line receives a voltage pulse from a voltage source and compresses the voltage pulse to generate a trigger signal. Compressing the voltage pulse includes increasing amplitude of the voltage pulse and decreasing length of the voltage pulse in time. An igniter receives the trigger signal and detonates an explosive responsive to receipt of the trigger signal.

  4. DOE Sustainability Reporting Open Line Help Call

    Broader source: Energy.gov [DOE]

    The Sustainability Performance Office (SPO) sponsors open line help calls to assist DOE sites and national laboratories with the annual sustainability reporting process. Representatives from the...

  5. MANAGING TIGHT BINDING RECEPTORS FOR NEW SPEARATIONS TECHNOLOGIES

    SciTech Connect (OSTI)

    DARYLE H BUSCH RICHARD S GIVENS

    2004-12-10

    Much of the earth's pollution involves compounds of the metallic elements, including actinides, strontium, cesium, technetium, and RCRA metals. Metal ions bind to molecules called ligands, which are the molecular tools that can manipulate the metal ions under most conditions. This DOE-EMSP sponsored program strives (1) to provide the foundations for using the most powerful ligands in transformational separations technologies and (2) to produce seminal examples of their applications to separations appropriate to the DOE EM mission. These ultra tight-binding ligands can capture metal ions in the most competitive of circumstances (from mineralized sites, lesser ligands, and even extremely dilute solutions), but they react so slowly that they are useless in traditional separations methodologies. Two attacks on this problem are underway. The first accommodates to the challenging molecular lethargy by developing a seminal slow separations methodology termed the soil poultice. The second designs ligands that are only tight-binding while wrapped around the targeted metal ion, but can be put in place by switch-binding and removed by switch-release. We envision a kind of molecular switching process to accelerate the union between metal ion and tight-binding ligand. Molecular switching processes are suggested for overcoming the slow natural equilibration rate with which ultra tight-binding ligands combine with metal ions. Ligands that bind relatively weakly combine with metal ions rapidly, so the trick is to convert a ligand from a weak, rapidly binding species to a powerful, slow releasing ligand--during the binding of the ligand to the metal ion. Such switch-binding ligands must react with themselves, and the reaction must take place under the influence of the metal ion. For example, our generation 1 ligands showed that a well-designed linear ligand with ends that readily combine, forms a cyclic molecule when it wraps around a metal ion. Our generation 2 ligands are even more interesting. They convert from rings to structures that wrap around a metal ion to form a cage. These ligands are called cryptands. Switch release is accomplished by photolytic cleavage of a bond to convert a cyclic ligand into a linear ligand or to break similar bonds in a cryptate. Our studies have demonstrated switch binding and switch release with cryptates of calcium. These remarkable cyclic ligands and cage-like ligands are indeed tight-binding and may, in principle, be incorporated in various separations methodologies, including the soil poultice. The soil poultice mimics the way in which microbes secrete extremely powerful ligands into the soil in order to harvest iron. The cellular membrane of the microbe recognizes the iron/ligand complex and admits it into the cell. The soil poultice uses molecularly imprinted polymers (MIPs) to play the role of the cellular membrane. Imprinting involves creation of the polymer in the presence of the metal/ligand complex. In principle, a well design ligand/MIP combination can be highly selective toward almost any targeted metal ion. The principles for that design are the focus of these investigations. An imprinting molecule can interact with the polymer through any, some, or all of the so-called supramolecular modes; e.g., hydrogen bonding, electrostatic charge, minor ligand bonding, Pi-Pi stacking, and hydrophobic and van der Waals interactions. Historically these modes of binding have given MIPs only small re-binding capacities and very limited selectivities. This program has shown that each mode of interaction can be made more powerful than previously suspected and that combinations of different supramolecular interaction modes can produce remarkable synergisms. The results of this systematic study provide a firm foundation for tailoring molecular imprinted polymers for reclamation of specific metal ion, including those important to the DOE EM mission.

  6. Terahertz Quantum-Cascade Transmission-Line Metamaterials

    E-Print Network [OSTI]

    Tavallaee, Amir Ali

    2012-01-01

    Transmission-Line Metamaterials . . . . . . . Terahertz CRLHCRLH Transmission-Line Metamaterials . . . . . . . 112losses in negative-index metamaterials by optical parametric

  7. State preparation and metrology of nitrogen nuclear spin in diamond

    E-Print Network [OSTI]

    Bang, Kilhyun

    2012-01-01

    with the QML limit ? K,QML (solid line) and the SQL limit? K,SQL (dashed line). How to choose the optimum ? k isas standard quantum limit or SQL) ? SQL = O(1/ R). Quantum

  8. Automating Oceanography: A Robotic Surface Sensor Platform Combining Flexibility and Low-cost

    E-Print Network [OSTI]

    Mairs, Bryant

    2015-01-01

    speed Water Speed (m/s) Distance Traveled (km) Energy Use (Energy use per meter (solid blue line) and total mission distance (dashed green line) for different waterdifferent water speeds. This is determined by the energy use

  9. Magnetic Field Line Stickiness in Tokamaks

    E-Print Network [OSTI]

    Martins, Caroline G L; Caldas, I L

    2013-01-01

    We present simulated figures of the diverted magnetic field lines of the tokamak ITER, obtained by numerically integrating a Hamiltonian model with electrical currents in five wire loops and control coils. We show evidences of a sticky island embedded in the chaotic region near the divertor plates, which traps magnetic field lines for many toroidal turns increasing their connection lengths to these plates.

  10. Line geometry and electromagnetism I: basic structures

    E-Print Network [OSTI]

    D. H. Delphenich

    2013-09-11

    Some key notions of line geometry are recalled, along with their application to mechanics. It is then shown that most of the basic structures that one introduces in the pre-metric formulation of electromagnetism can be interpreted directly in terms of corresponding concepts in line geometry. The results are summarized in a table.

  11. Cesium D Line Data Daniel Adam Steck

    E-Print Network [OSTI]

    Steck, Daniel A.

    Cesium D Line Data Daniel Adam Steck Oregon Center for Optics and Department of Physics, University 1998. This is revision 2.1.4, 23 December 2010. Cite this document as: Daniel A. Steck, "Cesium D Line and optical properties of cesium that are relevant to various quantum optics experiments. In particular, we

  12. Migrating Automotive Product Lines: a Case Study

    E-Print Network [OSTI]

    Cordy, James R.

    . Software Product Lines (SPL) are widely used to manage variability in the automotive industry. In a rapidly study from an automotive domain, that it is tractable to lift industrial-grade transforma- tionsMigrating Automotive Product Lines: a Case Study Michalis Famelis1 , Levi L´ucio2 , Gehan Selim3

  13. Doering 6/2004 Freezing Cell Lines

    E-Print Network [OSTI]

    Doering, Tamara

    Doering 6/2004 Freezing Cell Lines Overview: This is crucial for maintaining important cell lines in the lab!!! Please freeze down any strain you or others might use in the future, whether that is a crypto personnel won't spend time reconstructing plasmids or strains that weren't kept. Instructions for freezing

  14. Speed-line for 3D animation 

    E-Print Network [OSTI]

    Song, Won Chan

    2007-04-25

    My thesis describes a tool which creates speed-lines automatically in 3D computer animations. Speed-lines are usually used in comic books to express fast motions in a still image. They are also used in 2D animations. Although animations don't need...

  15. Acid Gas Capture Using CO2-Binding Organic Liquids

    SciTech Connect (OSTI)

    Heldebrant, David J.; Koech, Phillip K.; Rainbolt, James E.; Zheng, Feng

    2010-11-10

    Current chemical CO2 scrubbing technology is primarily aqueous alkanolamine based. These systems rapidly bind CO2 (forming water-soluble carbamate and bicarbonate salts) however, the process has serious disadvantages. The concentration of monoethanolamine rarely exceeds 30 wt % due to the corrosive nature of the solution, and this reduces the maximum CO2 volumetric (?108 g/L) and gravimetric capacity (?7 wt%) of the CO2 scrubber. The ?30 wt % loading of ethanolamine also means that a large excess of water must be pumped and heated during CO2 capture and release, and this greatly increases the energy requirements especially considering the high specific heat of water (4 j/g-1K-1). Our approach is to switch to organic systems that chemically bind CO2 as liquid alkylcarbonate salts. Our CO2-binding organic liquids have higher CO2 solubility, lower specific heats, potential for less corrosion and lower binding energies for CO2 than aqueous systems. CO2BOLs also reversibly bind and release mixed sulfur oxides. Furthermore the CO2BOL system can be direct solvent replacements for any solvent based CO2 capture systems because they are commercially available reagents and because they are fluids they would not require extensive process re-engineering.

  16. Broad iron lines in Active Galactic Nuclei

    E-Print Network [OSTI]

    A. C. Fabian; K. Iwasawa; C. S. Reynolds; A. J. Young

    2000-04-26

    An intrinsically narrow line emitted by an accretion disk around a black hole appears broadened and skewed as a result of the Doppler effect and gravitational redshift. The fluorescent iron line in the X-ray band at 6.4-6.9keV is the strongest such line and is seen in the X-ray spectrum of many active galactic nuclei and, in particular, Seyfert galaxies. It is an important diagnostic with which to study the geometry and other properties of the accretion flow very close to the central black hole. The broad iron line indicates the presence of a standard thin accretion disk in those objects, often seen at low inclination. The broad iron line has opened up strong gravitational effects around black holes to observational study with wide-reaching consequences for both astrophysics and physics.

  17. Dynamic Structural Rearrangements Between DNA Binding Modes of E. coli SSB Protein

    E-Print Network [OSTI]

    Lohman, Timothy M.

    an oligonucleotide/oligosaccharide bind- ing (OB) fold,5,7­9 hence the tetramer has four po- tential ssDNA binding sites. The SSB tetramer can bind long ssDNA in a variety of binding modes depending on solutionCl) and high protein to DNA ratios, an SSB tetramer binds to ssDNA with high inter-tetramer cooperativity using

  18. Through the Looking Glass: Off-Line Versus On-Line Coverage of Planar Environments by

    E-Print Network [OSTI]

    Rimon, Elon

    is demonstrated. Due to its many applications, on-line coverage is a highly researched topic in the mobile robotThrough the Looking Glass: Off-Line Versus On-Line Coverage of Planar Environments by a Battery Powered Autonomous Mobile Robot Iddo Shnaps and Elon Rimon Dept. of ME, Technion, Israel Institute

  19. Ethan Burns (UNH) Anticipatory On-line Planning 1 / 22 Anticipatory On-line Planning

    E-Print Network [OSTI]

    Ruml, Wheeler

    Ethan Burns (UNH) Anticipatory On-line Planning ­ 1 / 22 Anticipatory On-line Planning Ethan Burns1 Formalization Hindsight Opt. Experiments Conclusion Ethan Burns (UNH) Anticipatory On-line Planning ­ 2 / 22'd s Contributions Formalization Hindsight Opt. Experiments Conclusion Ethan Burns (UNH) Anticipatory On

  20. The Cellulose-binding Domains from Cellulomonas fimi bbb-1,4-Glucanase CenC Bind Nitroxide Spin-labeled

    E-Print Network [OSTI]

    McIntosh, Lawrence P.

    The Cellulose-binding Domains from Cellulomonas fimi bbb-1,4-Glucanase CenC Bind Nitroxide Spin6T 1Z3, Canada The N-terminal cellulose-binding domains CBDN1 and CBDN2 from Cellulomonas ®mi cellulase CenC each adopt a jelly-roll b-sandwich struc- ture with a cleft into which amorphous cellulose

  1. Orientation-dependent binding energy of graphene on palladium

    SciTech Connect (OSTI)

    Kappes, Branden B.; Ebnonnasir, Abbas; Ciobanu, Cristian V. [Department of Mechanical Engineering and Materials Science Program, Colorado School of Mines, Golden, Colorado 80401 (United States)] [Department of Mechanical Engineering and Materials Science Program, Colorado School of Mines, Golden, Colorado 80401 (United States); Kodambaka, Suneel [Department of Materials Science and Engineering, University of California, Los Angeles, Los Angeles, California 90095 (United States)] [Department of Materials Science and Engineering, University of California, Los Angeles, Los Angeles, California 90095 (United States)

    2013-02-04

    Using density functional theory calculations, we show that the binding strength of a graphene monolayer on Pd(111) can vary between physisorption and chemisorption depending on its orientation. By studying the interfacial charge transfer, we have identified a specific four-atom carbon cluster that is responsible for the local bonding of graphene to Pd(111). The areal density of such clusters varies with the in-plane orientation of graphene, causing the binding energy to change accordingly. Similar investigations can also apply to other metal substrates and suggests that physical, chemical, and mechanical properties of graphene may be controlled by changing its orientation.

  2. Computing Criticality of Lines in Power Systems

    SciTech Connect (OSTI)

    Pinar, Ali; Reichert, Adam; Lesieutre, Bernard

    2006-10-13

    We propose a computationally efficient method based onnonlinear optimization to identify critical lines, failure of which cancause severe blackouts. Our method computes criticality measure for alllines at a time, as opposed to detecting a single vulnerability,providing a global view of the system. This information on criticality oflines can be used to identify multiple contingencies by selectivelyexploring multiple combinations of broken lines. The effectiveness of ourmethod is demonstrated on the IEEE 30 and 118 bus systems, where we canvery quickly detect the most critical lines in the system and identifysevere multiple contingencies.

  3. Temperatures in the blast furnace refractory lining

    SciTech Connect (OSTI)

    Hebel, R.; Streuber, C. [Didier-M and P Energietechnik GmbH, Wiesbaden (Germany); Steiger, R. [Didier-M and P Engineering Services, Highland, IN (United States); Jeschar, R. [TU Clausthal (Germany). Inst. fuer Energieverfahrenstechnik und Brennstofftechnik

    1995-12-01

    The campaign life duration of a blast furnace is mainly determined by the condition of the refractory lining in heavy-duty zones such as the hearth, bosh, belly and lower stack. To achieve a desired lifetime, the temperature of the lining in these areas thereby proved to be the decisive controllable parameter. Low operating temperatures result in prolonged service life and are attained through high cooling efficiency. Besides the refractory grade chosen, the wear profile is mainly determined by the type of cooling system applied and the cooling intensity. Therefore, an appropriate compromise between long service life and energy losses has to be found in each case. In order to predict the service life of a lining it is important to know the wear condition at all times during the campaign. The paper describes the approaches the authors have made so far on European blast furnaces, on a theoretical and practical basis, on how to analyze the lining wear.

  4. DNA Assembly Line for Nano-Construction

    ScienceCinema (OSTI)

    Oleg Gang

    2010-01-08

    Building on the idea of using DNA to link up nanoparticles scientists at Brookhaven National Lab have designed a molecular assembly line for high-precision nano-construction. Nanofabrication is essential for exploiting the unique properties of nanoparticl

  5. Line Environment, Safety and Health Oversight

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    1997-06-26

    Sets forth the Department's expectations line management environment, safety and health (ES&H) oversight and for the use of contractor self-assessment programs as the cornerstone for this oversight. Canceled by DOE O 226.1.

  6. Productivity Improvement of a Manual Assembly Line 

    E-Print Network [OSTI]

    Yerasi, Pranavi

    2012-10-19

    The current project addresses the productivity improvement of a manual assembly line by making use of operations analysis in the framework of Lean production. A methodology is proposed that helps to improve the productivity of any production process...

  7. Ames Lab 101: Next Generation Power Lines

    ScienceCinema (OSTI)

    Russell, Alan

    2012-08-29

    Ames Laboratory scientist Alan Russell discusses the need to develop new power lines that are stronger and more conductive as a way to address the problem of the nation's aging and inadequate power grid.

  8. Electrical Transmission Line Diametrical Retention Mechanism

    DOE Patents [OSTI]

    Hall, David R. (Provo, UT); Hall, Jr., H. Tracy (Provo, UT); Pixton, David (Lehi, UT); Dahlgren, Scott (Provo, UT); Sneddon, Cameron (Provo, UT); Briscoe, Michael (Lehi, UT); Fox, Joe (Spanish Fork, UT)

    2006-01-03

    The invention is a mechanism for retaining an electrical transmission line. In one embodiment of the invention it is a system for retaining an electrical transmission line within downhole components. The invention allows a transmission line to be attached to the internal diameter of drilling components that have a substantially uniform drilling diameter. In accordance with one aspect of the invention, the system includes a plurality of downhole components, such as sections of pipe in a drill string, drill collars, heavy weight drill pipe, and jars. The system also includes a coaxial cable running between the first and second end of a drill pipe, the coaxial cable having a conductive tube and a conductive core within it. The invention allows the electrical transmission line to withstand the tension and compression of drill pipe during routine drilling cycles.

  9. On-line chemical composition analyzer development

    SciTech Connect (OSTI)

    Garrison, A.A.

    1993-01-01

    This report relates to the development of an on-line Raman analyzer for control of a distillation column. It is divided into: program issues, experimental control system evaluation, energy savings analysis, and reliability analysis. (DLC)

  10. Line-of-Sight Networks Alan Frieze

    E-Print Network [OSTI]

    Kleinberg, Jon

    -of-sight restrictions. Here we propose a random-graph model incorporating both range limitations and line-of-sight con- 0329064, CNS-0403340, and BCS-0537606; work done in part while on sabbatical leave at Carnegie Mellon

  11. Electrician - Foreman II (Transmission Lines & Substations) ...

    Broader source: Energy.gov (indexed) [DOE]

    Region Transmission Lines and Substation Maintenance (G5200) 615 S. 43rd Avenue Phoenix, AZ 85009 Duty Location is Page, AZ Find out more about living conditions at this...

  12. Design of the ILC RTML Extraction Lines

    SciTech Connect (OSTI)

    Seletskiy, S.; Tenenbaum, P.; Walz, D.; Solyak, N.; /Fermilab

    2011-10-17

    The ILC [1] Damping Ring to the Main Linac beamline (RTML) contains three extraction lines (EL). Each EL can be used both for an emergency abort dumping of the beam and tune-up continual train-by-train extraction. Two of the extraction lines are located downstream of the first and second stages of the RTML bunch compressor, and must accept both compressed and uncompressed beam with energy spreads of 2.5% and 0.15%, respectively. In this paper we report on an optics design that allowed minimizing the length of the extraction lines while offsetting the beam dumps from the main line by the distance required for acceptable radiation levels in the service tunnel. The proposed extraction lines can accommodate beams with different energy spreads while at the same time providing the beam size acceptable for the aluminum dump window. The RTML incorporates three extraction lines, which can be used for either an emergency beam abort or for a train-by-train extraction. The first EL is located downstream of the Damping Ring extraction arc. The other two extraction lines are located downstream of each stage of the two-stage bunch compressor. The first extraction line (EL1) receives 5GeV beam with an 0.15% energy spread. The extraction line located downstream of the first stage of bunch compressor (ELBC1) receives both compressed and uncompressed beam, and therefore must accept beam with both 5 and 4.88GeV energy, and 0.15% and 2.5% energy spread, respectively. The extraction line located after the second stage of the bunch compressor (ELBC2) receives 15GeV beam with either 0.15 or 1.8% energy spread. Each of the three extraction lines is equipped with the 220kW aluminum ball dump, which corresponds to the power of the continuously dumped beam with 5GeV energy, i.e., the beam trains must be delivered to the ELBC2 dump at reduced repetition rate.

  13. Molecular Cell High-Affinity Binding of Chp1 Chromodomain

    E-Print Network [OSTI]

    Halazonetis, Thanos

    Molecular Cell Article High-Affinity Binding of Chp1 Chromodomain to K9 Methylated Histone H3, Chp1, and siRNAs derived from centro- meric repeats. Recruitment of RITS to centromeres has been establishment. Our crystal structure of Chp1's chromodomain in complex with a trimethylated lysine 9 H3 peptide

  14. Telomeres and telomere binding proteins in Arabidopsis thaliana 

    E-Print Network [OSTI]

    Shakirov, Yevgeniy Vitalievich

    2004-09-30

    . Additionally, two Arabidopsis genes, AtPot1 and AtPot2, were identified and characterized. The genes encode two single-strand telomeric DNA binding proteins. AtPot1 and AtPot2 proteins can homo- and heterodimerize in vitro. Pot1 protein predominantly localizes...

  15. CAR2 Displays Unique Ligand Binding and RXR Heterodimerization Characteristics

    E-Print Network [OSTI]

    Omiecinski, Curtis

    CAR2 Displays Unique Ligand Binding and RXR Heterodimerization Characteristics Scott S. Auerbach ABSTRACT: The constitutive androstane receptor (CAR; NR1I3) regulates the expression of genes involved in xenobiotic metabolism. Alternative splicing of the human CAR gene yields an array of mRNAs that encode

  16. Binding of Harvested Bacterial Exopolymers to the Surface of

    E-Print Network [OSTI]

    of the EPS showed that binding strength to calcite depended on the chemical nature of the polymer. Introduction Dissolution of calcium carbonate (calcite) affects global carbon cycling (1), the chemistry of calcium carbonate in the form of limestone or marble. Retardation of deterioration is important

  17. A probabilistic approach to microRNA-target binding

    SciTech Connect (OSTI)

    Ogul, Hasan; Umu, Sinan U.; Bioinformatics Program, Informatics Institute, Middle East Technical University, Cankaya TR-06800, Ankara ; Tuncel, Y. Yener; Akkaya, Mahinur S.

    2011-09-16

    Highlights: {yields} A new probabilistic model is introduced for microRNA-target binding. {yields} The new model significantly outperforms RNAHybrid and miRTif. {yields} The experiments can unveil the effects of the type and directions of distinct base pairings. -- Abstract: Elucidation of microRNA activity is a crucial step in understanding gene regulation. One key problem in this effort is how to model the pairwise interactions of microRNAs with their targets. As this interaction is strongly mediated by their sequences, it is desired to set-up a probabilistic model to explain the binding preferences between a microRNA sequence and the sequence of a putative target. To this end, we introduce a new model of microRNA-target binding, which transforms an aligned duplex to a new sequence and defines the likelihood of this sequence using a Variable Length Markov Chain. It offers a complementary representation of microRNA-mRNA pairs for microRNA target prediction tools or other probabilistic frameworks of integrative gene regulation analysis. The performance of present model is evaluated by its ability to predict microRNA-target mRNA interaction given a mature microRNA sequence and a putative mRNA binding site. In regard to classification accuracy, it outperforms two recent methods based on thermodynamic stability and sequence complementarity. The experiments can also unveil the effects of base pairing types and non-seed region in duplex formation.

  18. Expression and subcellular localisation of poly(A)-binding proteins 

    E-Print Network [OSTI]

    Burgess, Hannah

    2010-11-24

    Poly(A)-binding proteins (PABPs) are important regulators of mRNA translation and stability. In mammals four cytoplasmic PABPs with a similar domain structure have been described - PABP1, tPABP, PABP4 and ePABP. The ...

  19. Compilation as Metacomputation: Binding Time Separation in Modular Compilers

    E-Print Network [OSTI]

    Kamin, Sam

    Compilation as Metacomputation: Binding Time Separation in Modular Compilers (Extended Abstract­ ings. Metacomputation­style specification lends itself to semantics­directed compilation, which we demonstrate by creating a modular compiler for a higher­order, imperative, Algol­like language. Keywords

  20. Methods of use of cellulose binding domain proteins

    DOE Patents [OSTI]

    Shoseyov, O.; Shpiegl, I.; Goldstein, M.A.; Doi, R.H.

    1997-09-23

    A cellulose binding domain (CBD) having a high affinity for crystalline cellulose and chitin is disclosed, along with methods for the molecular cloning and recombinant production. Fusion products comprising the CBD and a second protein are likewise described. A wide range of applications are contemplated for both the CBD and the fusion products, including drug delivery, affinity separations, and diagnostic techniques. 16 figs.

  1. Workshop on gate valve pressure locking and thermal binding

    SciTech Connect (OSTI)

    Brown, E.J.

    1995-07-01

    The purpose of the Workshop on Gate Valve Pressure Locking and Thermal Binding was to discuss pressure locking and thermal binding issues that could lead to inoperable gate valves in both boiling water and pressurized water reactors. The goal was to foster exchange of information to develop the technical bases to understand the phenomena, identify the components that are susceptible, discuss actual events, discuss the safety significance, and illustrate known corrective actions that can prevent or limit the occurrence of pressure locking or thermal binding. The presentations were structured to cover U.S. Nuclear Regulatory Commission staff evaluation of operating experience and planned regulatory activity; industry discussions of specific events, including foreign experience, and efforts to determine causes and alleviate the affects; and valve vendor experience and recommended corrective action. The discussions indicated that identifying valves susceptible to pressure locking and thermal binding was a complex process involving knowledge of components, systems, and plant operations. The corrective action options are varied and straightforward.

  2. Host Cell Responses Induced by Hepatitis C Virus Binding

    E-Print Network [OSTI]

    Timmer, Jens

    Host Cell Responses Induced by Hepatitis C Virus Binding Xinhua Fang,1 Mirjam B. Zeisel,1 Jochen. Blum,1 and Thomas F. Baumert1,5 Initiation of hepatitis C virus (HCV) infection is mediated by docking nitiation of hepatitis C virus (HCV) infection is me- diated by docking of the viral envelope to the hepa

  3. Inhibition Of Call-Cell Binding By Kipid Assemblies

    DOE Patents [OSTI]

    Nagy, Jon O. (Rodeo, CA), Bargatze, Robert F. (Bozeman, MT)

    2003-12-16

    This invention relates generally to the field of therapeutic compounds designed to interfere between the binding of ligands and their receptors on cell surface. More specifically, it provides products and methods for inhibiting cell migration and activation using lipid assemblies with surface recognition elements that are specific for the receptors involved in cell migration and activation.

  4. Inhibition of cell-cell binding by lipid assemblies

    DOE Patents [OSTI]

    Nagy, Jon O. (Rodeo, CA); Bargatze, Robert F. (Bozeman, MT)

    2001-05-22

    This invention relates generally to the field of therapeutic compounds designed to interfere between the binding of ligands and their receptors on cell surface. More specifically, it provides products and methods for inhibiting cell migration and activation using lipid assemblies with surface recognition elements that are specific for the receptors involved in cell migration and activation.

  5. Automatic Generation of Tcl Bindings for C and C++ Libraries

    E-Print Network [OSTI]

    Heidrich, Wolfgang

    functions. This facility has been used to create a variety of language bindings for C libraries, ranging++ library functions to Tcl, is that they do not normally receive their arguments using this argc public member function, but since C(++) data can not be addressed directly from Tcl, string handles need

  6. Analysis of the Vertebrate Insulator Protein CTCF-Binding

    E-Print Network [OSTI]

    D. Green,4 Michael Q. Zhang,3 Victor V. Lobanenkov,2 and Bing Ren1, * 1 Ludwig Institute for Cancer by preventing the spread of heterochromatin and restricting transcriptional enhancers from acti- vation ex- pressed nuclear protein with 11-zinc finger (ZF) DNA- binding domain (Filippova et al., 1996

  7. The peanut lectin-binding glycoproteins of human epidermal keratinocytes

    SciTech Connect (OSTI)

    Morrison, A.I. (Max-Planck-Institute fuer Systemphysiologie, Dortmund (West Germany)); Keeble, S.; Watt, F.M. (Imperial Cancer Research Fund, London (England))

    1988-08-01

    The peanut lectin (PNA) is known to bind more strongly to keratinocytes that are undergoing terminal differentiation than to proliferating keratinocytes. In order to investigate the significance of this change in cell-surface carbohydrate authors have identified the PNA-binding glycoproteins of cultured human keratinocytes and antibodies against them. Two heavily glycosylated bands of 110 and 250 kDa were resolved by PAGE of ({sup 14}C)galactose- or ({sup 14}C)mannose- and ({sup 14}C)glucosamine-labeled cell extracts eluted with galactose from PNA affinity columns. The higher molecular weight band was also detected on PNA blots of unlabeled cell extracts transferred to nitrocellulose. Both bands were sensitive to pronase digestion, but only the 250-kDa band was digested with trypsin. A rabbit antiserum that we prepared (anti-PNA-gp) immunoprecipitated both bands from cell extracts. In contrast to PNA, anti-PNA-gp bound equally to proliferating and terminally differentiating cells, indicating that some epitope(s) of the PNA-binding glycoproteins is present on the cell surface prior to terminal differentiation. When keratinocytes grown as a monolayer in low-calcium medium were switched to medium containing 2 mM calcium ions in order to induce desmosome formation and stratification, there was a dramatic redistribution of the PNA-binding glycoproteins, which became concentrated at the boundaries between cells. This may suggest a role for the glycoproteins in cell-cell interactions during stratification.

  8. Methods of detection using a cellulose binding domain fusion product

    DOE Patents [OSTI]

    Shoseyov, Oded (Shimshon, IL); Shpiegl, Itai (North Gallilea, IL); Goldstein, Marc A. (Davis, CA); Doi, Roy H. (Davis, CA)

    1999-01-01

    A cellulose binding domain (CBD) having a high affinity for crystalline cellulose and chitin is disclosed, along with methods for the molecular cloning and recombinant production thereof. Fusion products comprising the CBD and a second protein are likewise described. A wide range of applications are contemplated for both the CBD and the fusion products, including drug delivery, affinity separations, and diagnostic techniques.

  9. Methods of use of cellulose binding domain proteins

    DOE Patents [OSTI]

    Shoseyov, Oded (Karmey Yosef, IL); Shpiegl, Itai (Rehovot, IL); Goldstein, Marc A. (Davis, CA); Doi, Roy H. (Davis, CA)

    1997-01-01

    A cellulose binding domain (CBD) having a high affinity for crystalline cellulose and chitin is disclosed, along with methods for the molecular cloning and recombinant production thereof. Fusion products comprising the CBD and a second protein are likewise described. A wide range of applications are contemplated for both the CBD and the fusion products, including drug delivery, affinity separations, and diagnostic techniques.

  10. Methods of detection using a cellulose binding domain fusion product

    DOE Patents [OSTI]

    Shoseyov, O.; Shpiegl, I.; Goldstein, M.A.; Doi, R.H.

    1999-01-05

    A cellulose binding domain (CBD) having a high affinity for crystalline cellulose and chitin is disclosed, along with methods for the molecular cloning and recombinant production. Fusion products comprising the CBD and a second protein are likewise described. A wide range of applications are contemplated for both the CBD and the fusion products, including drug delivery, affinity separations, and diagnostic techniques. 34 figs.

  11. Experiences with On-Line STEAMPOP 

    E-Print Network [OSTI]

    Nath, R.; Libby, D. J.; Marcinkowska, A. R.; Skarke, S. C.; Thomasson, M. J.; Worsham, B. A.

    1988-01-01

    manual step to complete the loop. The results of optimization have to be conveyed back to the plant computer responsible for assigning the control set points. In order to automate the above mentioned manual steps, the optimization program has...-Line STEAMPOP R. Nath, D. J. Libby, A. R. Marcinkowska, S. C. Skarke, M. J. Thomasson and B. A. Worsham Union Carbide Corporation Houston, Texas ABSTRACT Off-line utility system optimizers for large process plants are now common place [1...

  12. New Construction of Distribution Lines, Service Lines, and Appurtenant Facilities in Residential Subdivisions (New York)

    Broader source: Energy.gov [DOE]

    Any proposed construction of electricity-related facilities in residential subdivisions, including distribution and service lines and appurtenant facilities, is subject to these regulations, which...

  13. O.A.R. 734-055 - Pole Lines, Buried Cables, Pipe lines, Signs...

    Open Energy Info (EERE)

    and Operations (1981). Retrieved from "http:en.openei.orgwindex.php?titleO.A.R.734-055-PoleLines,BuriedCables,Pipelines,Signs,Misc.FacilitiesandOperations...

  14. Glucose oxidation in heart-type fatty acid binding protein null mice 

    E-Print Network [OSTI]

    Adhikari, Sean

    2006-10-30

    Heart-type fatty acid binding protein (H-FABP) is a major fatty acid binding factor in skeletal muscles. Genetic lack of H-FABP severely impairs the esterification and oxidation of exogenous fatty acids in soleus muscles ...

  15. Insight into Ligand Diversity and Novel Biological Roles for Family 32 Carbohydrate-Binding Modules

    E-Print Network [OSTI]

    Eirin Lopez, Jose Maria

    a new platform for the prediction of CBM binding specificity and highlight significant new targets et al. 2007). For example, Yersinia enterocolitica contains a family 32 car- bohydrate-binding module

  16. Expression and characterisation of a novel poly(A)-binding protein, PABP5 

    E-Print Network [OSTI]

    Anderson, Ross Calley

    2010-11-24

    The poly(A)-binding proteins (PABPs) are a family of eukaryotic RNA-binding proteins with key roles in mRNA translation and stability. The molecular function of PABPs have been largely revealed through study of the ...

  17. Common binding by redundant group B Sox proteins is evolutionarily conserved in Drosophila.

    E-Print Network [OSTI]

    Carl, Sarah H.; Russell, Steven

    2015-04-13

    Three-way comparison of Dichaete binding. (A) Pie chart showin present in one species (47%), two species (23%) or all three species (30%). in D. melanogaster, D. simulans and D. yakuba clustered by binding affinity key and histogram shows...

  18. Syntax-driven bindings of Spanish clitic pronoun Ivan V. Meza Ruiz

    E-Print Network [OSTI]

    Pineda, Luis

    ´on con la teor´ia de binding. Los pronombres cl´iticos del espa~nol presentan un comportamiento dual dado control de objeto. Palabras clave: Pronombres cl´iticos, Teor´ia de Binding, Reflexividad, HPSG Abstract

  19. Soil Moisture Impacts on Convective Precipitation in Oklahoma 

    E-Print Network [OSTI]

    Ford, Trenton

    2015-07-24

    of the States” instilled in me a lifelong curiosity in Geography. v NOMENCLATURE CAPE Convective Available Potential Energy CIN Convective Inhibition CTP Convective Triggering Potential DDR Directional Difference Ratio DM Dry Moderate DP Dry Polar.... The black line represents the 1-to-1 fit, while the dashed line represents the least squares fit. .………………………..119 Figure 5.3. Boxplots of DDR from each directional difference over dry soil events. The black, dashed line represents the 0 value, by which...

  20. Glosten Associates Tests Innovative High-Strength Mooring Lines...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Glosten Associates Tests Innovative High-Strength Mooring Lines for Deepwater Offshore Wind Installations Glosten Associates Tests Innovative High-Strength Mooring Lines for...

  1. Insulate Steam Distribution and Condensate Return Lines, Energy...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    2 Insulate Steam Distribution and Condensate Return Lines Uninsulated steam distribution and condensate return lines are a constant source of wasted energy. The table shows typical...

  2. Development of aerogel-lined targets for inertial confinement...

    Office of Scientific and Technical Information (OSTI)

    ThesisDissertation: Development of aerogel-lined targets for inertial confinement fusion experiments Citation Details In-Document Search Title: Development of aerogel-lined...

  3. COOH-terminal truncation of flightin decreases myofilament lattice organization, cross-bridge binding, and power output in Drosophila indirect flight muscle

    SciTech Connect (OSTI)

    Tanner, Bertrand C.W.; Miller, Mark S.; Miller, Becky M.; Lekkas, Panagiotis; Irving, Thomas C.; Maughan, David W.; Vigoreaux, Jim O. (IIT); (Vermont)

    2011-08-26

    The indirect flight muscle (IFM) of insects is characterized by a near crystalline myofilament lattice structure that likely evolved to achieve high power output. In Drosophila IFM, the myosin rod binding protein flightin plays a crucial role in thick filament organization and sarcomere integrity. Here we investigate the extent to which the COOH terminus of flightin contributes to IFM structure and mechanical performance using transgenic Drosophila expressing a truncated flightin lacking the 44 COOH-terminal amino acids (fln{sup {Delta}C44}). Electron microscopy and X-ray diffraction measurements show decreased myofilament lattice order in the fln{sup {Delta}C44} line compared with control, a transgenic flightin-null rescued line (fln{sup +}). fln{sup {Delta}C44} fibers produced roughly 1/3 the oscillatory work and power of fln{sup +}, with reduced frequencies of maximum work (123 Hz vs. 154 Hz) and power (139 Hz vs. 187 Hz) output, indicating slower myosin cycling kinetics. These reductions in work and power stem from a slower rate of cross-bridge recruitment and decreased cross-bridge binding in fln{sup {Delta}C44} fibers, although the mean duration of cross-bridge attachment was not different between both lines. The decreases in lattice order and myosin kinetics resulted in fln{sup {Delta}C44} flies being unable to beat their wings. These results indicate that the COOH terminus of flightin is necessary for normal myofilament lattice organization, thereby facilitating the cross-bridge binding required to achieve high power output for flight.

  4. Load-resistant coaxial transmission line

    DOE Patents [OSTI]

    Hall, David R.; Fox, Joe

    2006-01-03

    A transmission line for downhole tools that make up all or part of a tool string for drilling and production of oil, gas, and geothermal wells that can withstand the dynamic gravitational forces and other accelerations associated with downhole excavations. The transmission line has a metal tube, or outer conductor, that houses a coaxial wire inner conductor. A non-metallic dielectric material is interposed between the inner and outer conductors. The outer and inner conductors and the dielectric are sufficiently compressed together so that independent motion between them is abated. Compression of the components of the transmission line may be achieved by drawing the transmission through one or more dies in order to draw down the outer conductor onto the dielectric, or by expanding the inner conductor against the dielectric using a mandrel or hydraulic pressure. Non-metallic bead segments may be used in aid of the compression necessary to resist the dynamic forces and accelerations of drilling.

  5. Dynamic Line Rating: Research and Policy Evaluation

    SciTech Connect (OSTI)

    Jake P. Gentle; Kurt S. Myers; Michael R. West

    2014-07-01

    Dynamic Line Rating (DLR) is a smart grid technology that allows the rating of electrical conductors to be increased based on local weather conditions. Overhead lines are conventionally given a conservative rating based on worst case scenarios. We demonstrate that observing the conditions in real time leads to additional capacity and safer operation. This paper provides a report of a pioneering scheme in the United States of America in which DLR has been applied. Thereby, we demonstrate that observing the local weather conditions in real time leads to additional capacity and safer operation. Secondly, we discuss limitations involved. In doing so, we arrive at novel insights which will inform and improve future DLR projects. Third, we provide a policy background and discussion to clarify the technology’s potential and identifies barriers to the imminent adoption of dynamic line rating systems. We provide suggestions for regulatory bodies about possible improvements in policy to encourage adoption of this beneficial technology.

  6. Discovery and Characterization of a Cell-Permeable, Small-Molecule c-Abl Kinase Activator that Binds to the Myristoyl Binding Site

    SciTech Connect (OSTI)

    Yang, Jingsong; Campobasso, Nino; Biju, Mangatt P.; Fisher, Kelly; Pan, Xiao-Qing; Cottom, Josh; Galbraith, Sarah; Ho, Thau; Zhang, Hong; Hong, Xuan; Ward, Paris; Hofmann, Glenn; Siegfried, Brett; Zappacosta, Francesca; Washio, Yoshiaki; Cao, Ping; Qu, Junya; Bertrand, Sophie; Wang, Da-Yuan; Head, Martha S.; Li, Hu; Moores, Sheri; Lai, Zhihong; Johanson, Kyung; Burton, George; Erickson-Miller, Connie; Simpson, Graham; Tummino, Peter; Copeland, Robert A.; Oliff, Allen

    2014-10-02

    c-Abl kinase activity is regulated by a unique mechanism involving the formation of an autoinhibited conformation in which the N-terminal myristoyl group binds intramolecularly to the myristoyl binding site on the kinase domain and induces the bending of the {alpha}I helix that creates a docking surface for the SH2 domain. Here, we report a small-molecule c-Abl activator, DPH, that displays potent enzymatic and cellular activity in stimulating c-Abl activation. Structural analyses indicate that DPH binds to the myristoyl binding site and prevents the formation of the bent conformation of the {alpha}I helix through steric hindrance, a mode of action distinct from the previously identified allosteric c-Abl inhibitor, GNF-2, that also binds to the myristoyl binding site. DPH represents the first cell-permeable, small-molecule tool compound for c-Abl activation.

  7. The role of cooperative binding on noise expression

    E-Print Network [OSTI]

    P. S. Gutierrez; D. Monteoliva; L. Diambra

    2009-08-02

    The origin of stochastic fluctuations in gene expression has received considerable attention recently. Fluctuations in gene expression are particularly pronounced in cellular systems because of the small copy number of species undergoing transitions between discrete chemical states and the small size of biological compartments. In this paper, we propose a stochastic model for gene expression regulation including several binding sites, considering elementary reactions only. The model is used to investigate the role of cooperativity on the intrinsic fluctuations of gene expression, by means of master equation formalism. We found that the Hill coefficient and the level of noise increases as the interaction energy between activators increases. Additionally, we show that the model allows to distinguish between two cooperative binding mechanisms.

  8. Improved value for the silicon free exciton binding energy

    SciTech Connect (OSTI)

    Green, Martin A., E-mail: m.green@unsw.edu.au [Australian Centre for Advanced Photovoltaics, School of Photovoltaic and Renewable Energy Engineering, University of New South Wales, Sydney, Australia 2052 (Australia)

    2013-11-15

    The free exciton binding energy is a key parameter in silicon material and device physics. In particular, it provides the necessary link between the energy threshold for valence to conduction band optical absorption and the bandgap determining electronic properties. The long accepted low temperature binding energy value of 14.7 ± 0.4 meV is reassessed taking advantage of developments subsequent to its original determination, leading to the conclusion that this value is definitely an underestimate. Using three largely independent experimental data sets, an improved low temperature value of 15.01 ± 0.06 meV is deduced, in good agreement with the most comprehensive theoretical calculations to date.

  9. Syntheses and DNA binding of new cationic porphyrintetrapeptide conjugates Gbor Mez a

    E-Print Network [OSTI]

    Langowski, Jörg

    shifts and hypochromicities. Decomposition of absorption spectra suggested the formation of two of di- and tricationic porphyrins bind to DNA by two distinct binding modes which can be identi ed agents [6,7]. The binding of cationic porphyrin derivatives to nucleic acids has been the subject

  10. Promiscuous binding of extracellular peptides to cell surface class I MHC protein

    E-Print Network [OSTI]

    Eisen, Herman N.

    Algorithms derived from measurements of short-peptide (8–10 mers) binding to class I MHC proteins suggest that the binding groove of a class I MHC protein, such as K[superscript b], can bind well over 1 million different ...

  11. SFmap: a web server for motif analysis and prediction of splicing factor binding sites

    E-Print Network [OSTI]

    Mandel-Gutfreund, Yael

    SFmap: a web server for motif analysis and prediction of splicing factor binding sites Inbal Paz1) that bind to sequence elements on the RNA. SFmap is a web server for predicting putative SF binding sites by the user. SFmap results are provided both as a text file and as a graphical web interface. INTRODUCTION

  12. Ultrastrong Optical Binding of Metallic Nanoparticles Vassili Demergis and Ernst-Ludwig Florin*

    E-Print Network [OSTI]

    Texas at Austin. University of

    Ultrastrong Optical Binding of Metallic Nanoparticles Vassili Demergis and Ernst-Ludwig Florin the optical binding force, which has been assumed to be weak compared to the optical gradient and scattering forces. We show that trapping by the optical binding force can be over 20 times stronger than

  13. Theory of Free Energy and Entropy in Noncovalent Binding Huan-Xiang Zhou*,

    E-Print Network [OSTI]

    Weston, Ken

    Theory of Free Energy and Entropy in Noncovalent Binding Huan-Xiang Zhou*, and Michael K. Gilson, Rockville, Maryland 20850 Received December 23, 2008 Contents 1. Introduction 4092 2. Free Energy, Partition.4. Solvation and a Temperature-Dependent Energy Function 4096 3. Binding Free Energy and Binding Constant 4096

  14. Metal binding in proteins: machine learning complements X-ray absorption spectroscopy

    E-Print Network [OSTI]

    Passerini, Andrea

    Metal binding in proteins: machine learning complements X-ray absorption spectroscopy Marco Lippi1 for the identification of metalloproteins and metal binding sites on a genome scale. An extensive evaluation conducted in combination with X- ray absorption spectroscopy shows the great potentiality of the approach. 1 Metal binding

  15. Efficient Evaluation of Binding Free Energy Using Continuum Electrostatics Danzhi Huang and Amedeo Caflisch*

    E-Print Network [OSTI]

    Caflisch, Amedeo

    Efficient Evaluation of Binding Free Energy Using Continuum Electrostatics Solvation Danzhi Huang of the absolute free energy of binding. A predictive accuracy of about 1.0 kcal/mol is obtained for 13 and 29 into proteins of known structure require fast and accurate methods for the evaluation of binding free energies.1

  16. Non-binding of Flavor-Singlet Hadrons to Nuclei

    E-Print Network [OSTI]

    Glennys R. Farrar; Gabrijela Zaharijas

    2003-02-20

    Strongly attractive color forces in the flavor singlet channel may lead to a stable H dibaryon. Here we show that an H or other compact, flavor singlet hadron is unlikely to bind to nuclei, so that bounds on exotic isotopes do not exclude their stability. Remarkably, a stable H appears to evade other experimental constraints as well, when account is taken of its expected compact spatial wavefunction.

  17. Reversible Acid Gas Capture Using CO2-Binding Organic Liquids

    SciTech Connect (OSTI)

    Heldebrant, David J.; Koech, Phillip K.; Yonker, Clement R.; Rainbolt, James E.; Zheng, Feng

    2010-08-31

    Acid gas scrubbing technology is predominantly aqueous alkanolamine based. Of the acid gases, CO2, H2S and SO2 have been shown to be reversible, however there are serious disadvantages with corrosion and high regeneration costs. The primary scrubbing system composed of monoethanolamine is limited to 30% by weight because of the highly corrosive solution. This gravimetric limitation limits the CO2 volumetric (?108 g/L) and gravimetric capacity (?7 wt%) of the system. Furthermore the scrubbing system has a large energy penalty from pumping and heating the excess water required to dissolve the MEA bicarbonate salt. Considering the high specific heat of water (4 j/g-1K-1), low capacities and the high corrosion we set out to design a fully organic solvent that can chemically bind all acid gases i.e. CO2 as reversible alkylcarbonate ionic liquids or analogues thereof. Having a liquid acid gas carrier improves process economics because there is no need for excess solvent to pump and to heat. We have demonstrated illustrated in Figure 1, that CO2-binding organic liquids (CO2BOLs) have a high CO2 solubility paired with a much lower specific heat (<1.5 J/g-1K-1) than aqueous systems. CO2BOLs are a subsection of a larger class of materials known as Binding Organic Liquids (BOLs). Our BOLs have been shown to reversibly bind and release COS, CS2, and SO2, which we denote COSBOLS, CS2BOLs and SO2BOLs. Our BOLs are highly tunable and can be designed for post or pre-combustion gas capture. The design and testing of the next generation zwitterionic CO2BOLs and SO2BOLs are presented.

  18. Synthesis and Characterization of Polyamine Bicycles for Anion Binding

    E-Print Network [OSTI]

    Morehouse, Paula Kay

    2007-10-09

    ,4,12,15,18,26,31,39-octaazapentacyclo [13.13.13.1.6,101.20,241.33,37] tetratetraconta-6, 7,9,20(43), 21,23,33(42),34,36-nonaene], N(CH2CH2NHCH2-m-xylyl-CH2NHCH2CH2)3N), 12, is readily capable of being protonated at the bridgehead amines. As a result it is also capable of binding anions...

  19. High molecular weight polysaccharide that binds and inhibits virus

    DOE Patents [OSTI]

    Konowalchuk, Thomas W

    2014-01-14

    This invention provides a high molecular weight polysaccharide capable of binding to and inhibiting virus and related pharmaceutical formulations and methods on inhibiting viral infectivity and/or pathogenicity, as well as immunogenic compositions. The invention further methods of inhibiting the growth of cancer cells and of ameliorating a symptom of aging. Additionally, the invention provides methods of detecting and/or quantifying and/or isolating viruses.

  20. Challenges to Overcurrent Protection Devices under Line-line Faults in Solar Photovoltaic Arrays

    E-Print Network [OSTI]

    Lehman, Brad

    Challenges to Overcurrent Protection Devices under Line-line Faults in Solar Photovoltaic Arrays Ye-MA, LLC Newburyport, MA, US Abstract--Solar photovoltaic (PV) arrays behave distinctively from remain undetected, which could lead to reduced system efficiency, reduced system reliability, and even

  1. EC Transmission Line Risk Identification and Analysis

    SciTech Connect (OSTI)

    Bigelow, Tim S

    2012-04-01

    The purpose of this document is to assist in evaluating and planning for the cost, schedule, and technical project risks associated with the delivery and operation of the EC (Electron cyclotron) transmission line system. In general, the major risks that are anticipated to be encountered during the project delivery phase associated with the implementation of the Procurement Arrangement for the EC transmission line system are associated with: (1) Undefined or changing requirements (e.g., functional or regulatory requirements) (2) Underperformance of prototype, first unit, or production components during testing (3) Unavailability of qualified vendors for critical components Technical risks associated with the design and operation of the system are also identified.

  2. Boron-10 Lined Proportional Counter Wall Effects

    SciTech Connect (OSTI)

    Siciliano, Edward R.; Kouzes, Richard T.

    2012-05-01

    The Department of Energy Office of Nuclear Safeguards (NA-241) is supporting the project 'Coincidence Counting With Boron-Based Alternative Neutron Detection Technology' at Pacific Northwest National Laboratory (PNNL) for development of an alternative neutron coincidence counter. The goal of this project is to design, build and demonstrate a boron-lined proportional tube based system in the configuration of a coincidence counter. This report provides information about how variations in proportional counter radius and gas pressure in a typical coincident counter design might affect the observed signal from boron-lined tubes. A discussion comparing tubes to parallel plate counters is also included.

  3. Concurrent Wind Cooling in Power Transmission Lines

    SciTech Connect (OSTI)

    Jake P Gentle

    2012-08-01

    Idaho National Laboratory and the Idaho Power Company, with collaboration from Idaho State University, have been working on a project to monitor wind and other environmental data parameters along certain electrical transmission corridors. The combination of both real-time historical weather and environmental data is being used to model, validate, and recommend possibilities for dynamic operations of the transmission lines for power and energy carrying capacity. The planned results can also be used to influence decisions about proposed design criteria for or upgrades to certain sections of the transmission lines.

  4. Binding energy of the $X(3872)$ at unphysical pion masses

    E-Print Network [OSTI]

    Baru, V; Filin, A A; Gegelia, J; Nefediev, A V

    2015-01-01

    Chiral extrapolation of the $X(3872)$ binding energy is investigated using the modified Weinberg formulation of chiral effective field theory for the $D \\bar{D}^*$ scattering. Given its explicit renormalisability, this approach is particularly useful to explore the interplay of the long- and short-range $D \\bar{D}^*$ forces in the $X(3872)$ from studying the light-quark (pion) mass dependence of its binding energy. In particular, the parameter-free leading-order calculation shows that the $X$-pole disappears for unphysical large pion masses. On the other hand, without contradicting the naive dimensional analysis, the higher-order pion-mass-dependent contact interaction can change the slope of the binding energy at the physical point yielding the opposite scenario of a stronger bound $X$ at pion masses larger than its physical value. An important role of the pion dynamics and of the 3-body $D\\bar{D}\\pi$ effects for chiral extrapolations of the $X$-pole is emphasised. The results of the present study should be ...

  5. Gold Binding by Native and Chemically Modified Hops Biomasses

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    López, M. Laura; Gardea-Torresdey, J. L.; Peralta-Videa, J. R.; de la Rosa, G.; Armendáriz, V.; Herrera, I.; Troiani, H.; Henning, J.

    2005-01-01

    Heavy metals from mining, smelting operations and other industrial processing facilities pollute wastewaters worldwide. Extraction of metals from industrial effluents has been widely studied due to the economic advantages and the relative ease of technical implementation. Consequently, the search for new and improved methodologies for the recovery of gold has increased. In this particular research, the use of cone hops biomass ( Humulus lupulus ) was investigated as a new option for gold recovery. The results showed that the gold binding to native hops biomass was pH dependent from pH 2 to pH 6, with a maximum percentage bindingmore »at pH 3. Time dependency studies demonstrated that Au(III) binding to native and modified cone hops biomasses was found to be time independent at pH 2 while at pH 5, it was time dependent. Capacity experiments demonstrated that at pH 2, esterified hops biomass bound 33.4 mg Au/g of biomass, while native and hydrolyzed hops biomasses bound 28.2 and 12.0 mg Au/g of biomass, respectively. However, at pH 5 the binding capacities were 38.9, 37.8 and 11.4 mg of Au per gram of native, esterified and hydrolyzed hops biomasses, respectively. « less

  6. Three-phase contact line and line tension of electrolyte solutions in contact with charged substrates

    E-Print Network [OSTI]

    Ingrid Ibagon; Markus Bier; S. Dietrich

    2015-11-16

    The three-phase contact line formed by the intersection of a liquid-vapor interface of an electrolyte solution with a charged planar substrate is studied in terms of classical density functional theory applied to a lattice model. The influence of the substrate charge density and of the ionic strength of the solution on the intrinsic structure of the three-phase contact line and on the corresponding line tension is analyzed. We find a negative line tension for all values of the surface charge density and of the ionic strength considered. The strength of the line tension decreases upon decreasing the contact angle via varying either the temperature or the substrate charge density.

  7. Sex Hormones Genotypes and Phenotypes and Determinants of Sex Hormone Binding Globulin in relation to Type 2 Diabetes Risk

    E-Print Network [OSTI]

    GOTO, ATSUSHI

    2012-01-01

    Consumption in Relation to Sex Hormone–Binding Globulin andin Relation to Circulating Sex Hormone-binding GlobulinReferences 4. Genes in Sex Hormone Pathways and Diabetes

  8. ABSTRACT OF THESIS LINE ASSOCIATIVE REGISTERS

    E-Print Network [OSTI]

    Dietz, Henry G. "Hank"

    ABSTRACT OF THESIS LINE ASSOCIATIVE REGISTERS As technological advances have improved processor. This thesis takes the approach of trying to create a new memory access model that is more efficient and simple ASSOCIATIVE REGISTERS By Krishna Melarkode Dr. Henry G. Dietz (Director of Thesis) Dr. Yu Ming Zhang (Director

  9. Appendix A Cesium D Line Data

    E-Print Network [OSTI]

    Steck, Daniel A.

    Appendices 249 #12;#12;Appendix A Cesium D Line Data A.1 Overview In this appendix we review many of the physical and optical properties of cesium that are relevant to the experiments in this dissertation. In particular, we give parameters that are useful in treating the mechanical effects of light on cesium atoms

  10. Cesium D Line Data Daniel A. Steck

    E-Print Network [OSTI]

    Steck, Daniel A.

    Cesium D Line Data Daniel A. Steck Theoretical Division (T-8), MS B285 Los Alamos National In this reference we present many of the physical and optical properties of cesium that are relevant to various effects of light on cesium atoms. The measured numbers are given with their original references

  11. Pipelined Asynchronous Circuits Andrew Matthew Lines

    E-Print Network [OSTI]

    Pipelined Asynchronous Circuits Andrew Matthew Lines June 1995, revised June 1998 This thesis building blocks for highly pipelined designs. The first chapter presents the implementation approach for individual cells. The second chapter in­ vestigates the time behavior of complex pipelined circuits

  12. High temperature lined conduits, elbows and tees

    DOE Patents [OSTI]

    De Feo, Angelo (Passaic, NJ); Drewniany, Edward (Bergen, NJ)

    1982-01-01

    A high temperature lined conduit comprising, a liner, a flexible insulating refractory blanket around and in contact with the liner, a pipe member around the blanket and spaced therefrom, and castable rigid refractory material between the pipe member and the blanket. Anchors are connected to the inside diameter of the pipe and extend into the castable material. The liner includes male and female slip joint ends for permitting thermal expansion of the liner with respect to the castable material and the pipe member. Elbows and tees of the lined conduit comprise an elbow liner wrapped with insulating refractory blanket material around which is disposed a spaced elbow pipe member with castable refractory material between the blanket material and the elbow pipe member. A reinforcing band is connected to the elbow liner at an intermediate location thereon from which extend a plurality of hollow tubes or pins which extend into the castable material to anchor the lined elbow and permit thermal expansion. A method of fabricating the high temperature lined conduit, elbows and tees is also disclosed which utilizes a polyethylene layer over the refractory blanket after it has been compressed to maintain the refractory blanket in a compressed condition until the castable material is in place. Hot gases are then directed through the interior of the liner for evaporating the polyethylene and setting the castable material which permits the compressed blanket to come into close contact with the castable material.

  13. Passive health monitoring of pressurized sensing lines

    SciTech Connect (OSTI)

    Holbert, K. E.; Lin, K. [Dept. of Electrical Engineering, Arizona State Univ., Tempe, AZ 85287-5706 (United States)

    2006-07-01

    The electrical-hydraulic analogy is employed for the modeling of pressurized lines. Anomalies such as leakage and blockage alter the normal system transfer function in such a manner that fault detection and identification can be performed. An impetus for this work is to incorporate simple algorithms within micro-electromechanical systems (MEMS) pressure transducers in order to create an intelligent sensor. (authors)

  14. Where are the Fermi lines coming from?

    SciTech Connect (OSTI)

    Rao, Kanishka; Whiteson, Daniel, E-mail: krao@uci.edu, E-mail: daniel@uci.edu [Dept. of Physics and Astronomy, UC Irvine, Irvine, CA (United States)

    2013-03-01

    We estimate the spatial locations of sources of the the observed features in the Fermi-LAT photon spectrum at E{sub ?} = 110 and E{sub ?} = 130 GeV. We determine whether they are consistent with emission from a single source, as would be expected in their interpretation as ?? and ?Z lines from dark matter annhiliation, as well as whether they are consistent with a dark matter halo positioned at the center of the galaxy. We take advantage of the per-photon measured incident angle in reconstructing the line features. In addition, we use a data-driven background model rather than making the assumption of a feature-less background. We localize the sources of the features at 110 and 130 GeV. Assuming an Einasto (NFW) density model we find the 130 GeV line to be offset from the Galactic center by 285 (280) pc, the 110 GeV line by 60 (30) pc with a large relative separation of 220 (240) pc. However, we find this displacement of each source from the Galactic center, as well as their relative displacement to be statistically consistent with a single Einasto or NFW dark matter halo at the center of the galaxy.

  15. The Linux Command Line Second Internet Edition

    E-Print Network [OSTI]

    Dahl, David B.

    The Linux Command Line Second Internet Edition William E. Shotts, Jr. A LinuxCommand.org Book #12 this book in elec- tronic formats for most popular e-readers: http://nostarch.com/tlcl.htm Release History Version Date Description 13.07 July 6, 2013 Second Internet Edition. 09.12 December 14, 2009 First

  16. Massachusetts Institute of Technology Center for Space Research

    E-Print Network [OSTI]

    denote gate valves. The vertical dashed line marks the interface between MIT and GSFC equipment. Further particles leaving the accelerator pass through an analyzing magnet which selects particles with a charge

  17. Supporting Information "Comparison of photoluminescence properties of semiconductor quantum dots and non-

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    microscope. The Hanburry Brown an Twiss time correlation system is depicted in the black dashed-line box, Andor Technology, Ireland). The Hanbury Brown and Twiss time-intensity correlation setup consist in two

  18. Revealing a Novel Mode of Action for an Osteoporosis Drug

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Bone beams were subjected to 4-point bending, and after each displacement of 20 mm (black arrow), 20 x-ray scattering measurements were taken (red dashed line). Diffraction...

  19. Microsoft Word - NETL-TRS-5-2014_High-Temperature, High-Pressure...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    values at 533 K (gray dashed line). Suggested Citation: Gamwo, I. K.; Tapriyal, D.; Enick, R. M.; McHugh, M. A.; Morreale, B. D. High Temperature, High Pressure Equation of...

  20. Hydrodynamics and Water Quality in Rodeo Lagoon, a Hypereutrophic Coastal Lagoon

    E-Print Network [OSTI]

    Cousins, Mary Alice Melugin

    2010-01-01

    lagoon (Fig. 2-4). Solar radiation data was provided by theand solar August 2007 radiation (dashed line). Wind data aredata for photoinhibition, the process in which bright solar radiation

  1. Line Broadening of EUV lines Across the Solar limb: A Spicule Contribution?

    E-Print Network [OSTI]

    to the dissipation of wave energy. However, O'Shea et al. (2005) has shown that the line widths start to show, Greece 3 School of Earth and Space Sciences, University of Science and Technology of China, Hefei, Anhui

  2. DNA-Binding Mechanism in Prokaryotic Partition Complex Formation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration would like submit theCovalent Bonding Low-Cost Ground8 GasDEVELOPMENTS E P I IT h itDNA-Binding

  3. Vibrational Softening of a Protein on Ligand Binding

    SciTech Connect (OSTI)

    Balog, Erica [Semmelweis University, Budapest, Hungary; Perahia, David [Ecole Normale Superieure de Cachan, Cachan, France; Smith, Jeremy C [ORNL; Merzel, Franci [National Institute of Chemistry, Solvenia

    2011-01-01

    Neutron scattering experiments have demonstrated that binding of the cancer drug methotrexate softens the low-frequency vibrations of its target protein, dihydrofolate reductase (DHFR). Here, this softening is fully reproduced using atomic detail normal-mode analysis. Decomposition of the vibrational density of states demonstrates that the largest contributions arise from structural elements of DHFR critical to stability and function. Mode-projection analysis reveals an increase of the breathing-like character of the affected vibrational modes consistent with the experimentally observed increased adiabatic compressibility of the protein on complexation.

  4. Towards engineering hormone-binding globulins as drug delivery agents

    E-Print Network [OSTI]

    Chan, Wee Lee; Zhou, Aiwu; Read, Randy J.

    2014-11-26

    (2014) Towards Engineering Hormone-Binding Globulins as Drug Delivery Agents. PLoS ONE 9(11): e113402. doi:10.1371/journal.pone. 0113402 Editor: Ashley M. Buckle, Monash University, Australia Received: June 10, 2014 Accepted: October 24, 2014 Published... stock solution was prepared by dissolving lyophilised cortisol (Sigma Aldrich) in 80% ethanol to make a 500 mM solution, which was then diluted with water to make solutions of 20, 40 and 80 mM, with water being used as a negative control. Aliquots...

  5. Hydroxyapatite-binding peptides for bone growth and inhibition

    DOE Patents [OSTI]

    Bertozzi, Carolyn R. (Berkeley, CA); Song, Jie (Shrewsbury, MA); Lee, Seung-Wuk (Walnut Creek, CA)

    2011-09-20

    Hydroxyapatite (HA)-binding peptides are selected using combinatorial phage library display. Pseudo-repetitive consensus amino acid sequences possessing periodic hydroxyl side chains in every two or three amino acid sequences are obtained. These sequences resemble the (Gly-Pro-Hyp).sub.x repeat of human type I collagen, a major component of extracellular matrices of natural bone. A consistent presence of basic amino acid residues is also observed. The peptides are synthesized by the solid-phase synthetic method and then used for template-driven HA-mineralization. Microscopy reveal that the peptides template the growth of polycrystalline HA crystals .about.40 nm in size.

  6. Functionalized polymers for binding to solutes in aqueous solutions

    DOE Patents [OSTI]

    Smith, Barbara F.; Robison, Thomas W.

    2006-11-21

    A functionalized polymer for binding a dissolved molecule in an aqueous solution is presented. The polymer has a backbone polymer to which one or more functional groups are covalently linked. The backbone polymer can be such polymers as polyethylenimine, polyvinylamine, polyallylamine, and polypropylamine. These polymers are generally water-soluble, but can be insoluble when cross-linked. The functional group can be for example diol derivatives, polyol derivatives, thiol and dithiol derivatives, guest-host groups, affinity groups, beta-diphosphonic acids, and beta-diamides

  7. Characterization of Selective Binding of Alkali Cations with Carboxylate

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 OutreachProductswsicloudwsiclouddenDVA N C E D BGene Network ShapingDate:Characterization of Selective Binding of

  8. Line-of-sight deposition method

    DOE Patents [OSTI]

    Patten, J.W.; McClanahan, E.D.; Bayne, M.A.

    1980-04-16

    A line-of-sight method of depositing a film having substantially 100% of theoretical density on a substrate. A pressure vessel contains a target source having a surface thereof capable of emitting particles therefrom and a substrate with the source surface and the substrate surface positioned such that the source surface is substantially parallel to the direction of the particles impinging upon the substrate surface, the distance between the most remote portion of the substrate surface receiving the particles and the source surface emitting the particles in a direction parallel to the substrate surface being relatively small. The pressure in the vessel is maintained less than about 5 microns to prevent scattering and permit line-of-sight deposition. By this method the angles of incidence of the particles impinging upon the substrate surface are in the range of from about 45/sup 0/ to 90/sup 0/ even when the target surface area is greatly expanded to increase the deposition rate.

  9. In-line real time air monitor

    DOE Patents [OSTI]

    Wise, Marcus B. (Kingston, TN); Thompson, Cyril V. (Knoxville, TN)

    1998-01-01

    An in-line gas monitor capable of accurate gas composition analysis in a continuous real time manner even under strong applied vacuum conditions operates by mixing an air sample with helium forming a sample gas in two complementary sample loops embedded in a manifold which includes two pairs of 3-way solenoid valves. The sample gas is then analyzed in an ion trap mass spectrometer on a continuous basis. Two valve drivers actuate the two pairs of 3-way valves in a reciprocating fashion, so that there is always flow through the in-line gas monitor via one or the other of the sample loops. The duty cycle for the two pairs of 3-way valves is varied by tuning the two valve drivers to a duty cycle typically between 0.2 to 0.7 seconds.

  10. In-line real time air monitor

    DOE Patents [OSTI]

    Wise, M.B.; Thompson, C.V.

    1998-07-14

    An in-line gas monitor capable of accurate gas composition analysis in a continuous real time manner even under strong applied vacuum conditions operates by mixing an air sample with helium forming a sample gas in two complementary sample loops embedded in a manifold which includes two pairs of 3-way solenoid valves. The sample gas is then analyzed in an ion trap mass spectrometer on a continuous basis. Two valve drivers actuate the two pairs of 3-way valves in a reciprocating fashion, so that there is always flow through the in-line gas monitor via one or the other of the sample loops. The duty cycle for the two pairs of 3-way valves is varied by tuning the two valve drivers to a duty cycle typically between 0.2 to 0.7 seconds. 3 figs.

  11. Multi-transmission-line-beam interactive system

    E-Print Network [OSTI]

    Alexander Figotin; Guillermo Reyes

    2013-09-04

    We construct here a Lagrangian field formulation for a system consisting of an electron beam interacting with a slow-wave structure modeled by a possibly non-uniform multiple transmission line (MTL). In the case of a single line we recover the linear model of a traveling wave tube (TWT) due to J.R. Pierce. Since a properly chosen MTL can approximate a real waveguide structure with any desired accuracy, the proposed model can be used in particular for design optimization. Furthermore, the Lagrangian formulation provides for: (i) a clear identification of the mathematical source of amplification, (ii) exact expressions for the conserved energy and its flux distributions obtained from the Noether theorem. In the case of uniform MTLs we carry out an exhaustive analysis of eigenmodes and find sharp conditions on the parameters of the system to provide for amplifying regimes.

  12. Boron-10 Lined Proportional Counter Model Validation

    SciTech Connect (OSTI)

    Lintereur, Azaree T.; Siciliano, Edward R.; Kouzes, Richard T.

    2012-06-30

    The Department of Energy Office of Nuclear Safeguards (NA-241) is supporting the project “Coincidence Counting With Boron-Based Alternative Neutron Detection Technology” at Pacific Northwest National Laboratory (PNNL) for the development of an alternative neutron coincidence counter. The goal of this project is to design, build and demonstrate a boron-lined proportional tube-based alternative system in the configuration of a coincidence counter. This report discusses the validation studies performed to establish the degree of accuracy of the computer modeling methods current used to simulate the response of boron-lined tubes. This is the precursor to developing models for the uranium neutron coincidence collar under Task 2 of this project.

  13. Giant vacuum forces via transmission lines

    E-Print Network [OSTI]

    Ephraim Shahmoon; Igor Mazets; Gershon Kurizki

    2014-07-23

    Quantum electromagnetic fluctuations induce forces between neutral particles, known as the van der Waals (vdW) and Casimir interactions. These fundamental forces, mediated by virtual photons from the vacuum, play an important role in basic physics and chemistry, and in emerging technologies involving, e.g. micro-electromechanical systems or quantum information processing. Here we show that these interactions can be enhanced by many orders of magnitude upon changing the character of the mediating vacuum-modes. By considering two polarizable particles in the vicinity of any standard electric transmission line, along which photons can propagate in one dimension (1d), we find a much stronger and longer-range interaction than in free-space. This enhancement may have profound implications on many-particle and bulk systems, and impact the quantum technologies mentioned above. The predicted giant vacuum force is estimated to be measurable in a coplanar waveguide line.

  14. Heavy rains hamper Louisiana gas line

    SciTech Connect (OSTI)

    Horner, C.

    1983-06-01

    Despite heavy rains and flooding a 36-mile gas pipeline loop for Transcontinental Gas Pipe Line Corp. was completed from north of Starks (at the end of Transco's south Louisiana lateral) to the Lake Charles area. Somastic-coated, 42-in. grade X-60 pipe comprises 90% of the route. The contract included multiple 30-42 in. fabrications, installation of six 42-in. gate valves, and expansion of the Gillis compressor station.

  15. Gas lines chasing huge northeastern market

    SciTech Connect (OSTI)

    Watts, J.

    1982-03-01

    Gas for the Northeastern US market is the driving force behind three proposed projects to bring Canadian gas to the New England-New York area: the 360-mile New England States pipeline (Algonquin Gas Transmission Co., Transcontinental Gas Pipe Line Corp., Texas Eastern Transmission Corp., and Nova, an Alberta Corp.); the 261-mile Boundary Gas project (with Boundary Gas Inc., a consortium of 14 gas utilities with Tennessee Gas Pipeline Co. providing transportation); and the 158-mile Niagara pipeline (Transcontinental Gas Pipe Line Corp.). Although none has yet received government (US and Canadian) approval, at least one project - the New England States line - is expected to be operational by 1984, bringing 305 million CF of natural gas daily for US residential and industrial markets. Both countries stand to benefit from the three projects. For Canada, the sale of gas to New England provides a steady market for massive quantities of gas makes building a pipeline from gas-rich Alberta (that will also serve eastern Canada) economically feasible, and ensures the existence of a transportation network in the Maritime provinces for use when production begins off Newfoundland and Nova Scotia. For the US, the gas from Canada will help reduce the nation's dependence on foreign oil and provide additional supplies during the peakload winter season.

  16. Pathogenicity of the BRCA1 Missense Variant M1775K is Determined by the Disruption of the BRCT Phosphopeptide-Binding Pocket: a Multi-Modal Approach

    SciTech Connect (OSTI)

    Tischkowitz,M.; Hamel, N.; Carvalho, M.; Birrane, G.; Soni, A.; van Beers, E.; Joosse, S.; Wong, N.; Novak, D.; et al

    2008-01-01

    A number of germ-line mutations in the BRCA1 gene confer susceptibility to breast and ovarian cancer. However, it remains difficult to determine whether many single amino-acid (missense) changes in the BRCA1 protein that are frequently detected in the clinical setting are pathologic or not. Here, we used a combination of functional, crystallographic, biophysical, molecular and evolutionary techniques, and classical genetic segregation analysis to demonstrate that the BRCA1 missense variant M1775K is pathogenic. Functional assays in yeast and mammalian cells showed that the BRCA1 BRCT domains carrying the amino-acid change M1775K displayed markedly reduced transcriptional activity, indicating that this variant represents a deleterious mutation. Importantly, the M1775K mutation disrupted the phosphopeptide-binding pocket of the BRCA1 BRCT domains, thereby inhibiting the BRCA1 interaction with the proteins BRIP1 and CtIP, which are involved in DNA damage-induced checkpoint control. These results indicate that the integrity of the BRCT phosphopeptide-binding pocket is critical for the tumor suppression function of BRCA1. Moreover, this study demonstrates that multiple lines of evidence obtained from a combination of functional, structural, molecular and evolutionary techniques, and classical genetic segregation analysis are required to confirm the pathogenicity of rare variants of disease-susceptibility genes and obtain important insights into the underlying pathogenetic mechanisms.

  17. Relativistic Nuclear Energy Density Functionals: adjusting parameters to binding energies

    E-Print Network [OSTI]

    T. Niksic; D. Vretenar; P. Ring

    2008-09-08

    We study a particular class of relativistic nuclear energy density functionals in which only nucleon degrees of freedom are explicitly used in the construction of effective interaction terms. Short-distance (high-momentum) correlations, as well as intermediate and long-range dynamics, are encoded in the medium (nucleon density) dependence of the strength functionals of an effective interaction Lagrangian. Guided by the density dependence of microscopic nucleon self-energies in nuclear matter, a phenomenological ansatz for the density-dependent coupling functionals is accurately determined in self-consistent mean-field calculations of binding energies of a large set of axially deformed nuclei. The relationship between the nuclear matter volume, surface and symmetry energies, and the corresponding predictions for nuclear masses is analyzed in detail. The resulting best-fit parametrization of the nuclear energy density functional is further tested in calculations of properties of spherical and deformed medium-heavy and heavy nuclei, including binding energies, charge radii, deformation parameters, neutron skin thickness, and excitation energies of giant multipole resonances.

  18. Binding and structure of tetramers in the scaling limit

    E-Print Network [OSTI]

    Hadizadeh, M R; Tomio, L; Delfino, A; Frederico, T

    2011-01-01

    The momentum-space structure of the Faddeev-Yakubovsky (FY) components of weakly-bound tetramers is investigated at the unitary limit using a renormalized zero-range two-body interaction. The results, obtained by considering a given trimer level with binding energy $B_3$, provide further support to a universal scaling function relating the binding energies of two successive tetramer states. The correlated scaling between the tetramer energies comes from the sensitivity of the four-boson system to a short-range four-body scale. Each excited $N-$th tetramer energy $B_4^{(N)}$ moves as the short-range four-body scale changes, while the trimer properties are kept fixed, with the next excited tetramer $B_4^{(N+1)}$ emerging from the atom-trimer threshold for a universal ratio $B_4^{(N)}/B_3 = B_4^ {(N)}/B_4^{(N+1)} \\simeq 4.6$, which does not depend on $N$. We show that both channels of the FY decomposition [atom-trimer ($K-$type) and dimer-dimer ($H-$type)] present high momentum tails, which reflect the short-ran...

  19. Binding and structure of tetramers in the scaling limit

    E-Print Network [OSTI]

    M. R. Hadizadeh; M. T. Yamashita; L. Tomio; A. Delfino; T. Frederico

    2012-01-17

    The momentum-space structure of the Faddeev-Yakubovsky (FY)components of weakly-bound tetramers is investigated at the unitary limit using a renormalized zero-range two-body interaction. The results, obtained by considering a given trimer level with binding energy $B_3$, provide further support to a universal scaling function relating the binding energies of two successive tetramer states. The correlated scaling between the tetramer energies comes from the sensitivity of the four-boson system to a short-range four-body scale. Each excited $N-$th tetramer energy $B_4^{(N)}$ moves as the short-range four-body scale changes, while the trimer properties are kept fixed, with the next excited tetramer $B_4^{(N+1)}$ emerging from the atom-trimer threshold for a universal ratio $B_4^{(N)}/B_3 = B_4^ {(N)}/B_4^{(N+1)} \\simeq 4.6$, which does not depend on $N$. We show that both channels of the FY decomposition [atom-trimer ($K-$type) and dimer-dimer ($H-$type)] present high momentum tails, which reflect the short-range four-body scale. We also found that the $H-$channel is favored over $K-$channel at low momentum when the four-body momentum scale largely overcomes the three-body one.

  20. Binding of transcription factors adapts to resolve information-energy trade-off

    E-Print Network [OSTI]

    Kagan, Yonatan Savir Jacob

    2015-01-01

    We examine the binding of transcription factors to DNA in terms of an information transfer problem. The input of the noisy channel is the biophysical signal of a factor bound to a DNA site, and the output is a distribution of probable DNA sequences at this site. This task involves an inherent tradeoff between the information gain and the energetics of the binding interaction - high binding energies provide higher information gain but hinder the dynamics of the system as factors are bound too tightly. We show that adaptation of the binding interaction towards increasing information transfer under energy constraints implies that the information gain per specific binding energy at each base-pair is maximized. We analyze hundreds of prokaryote and eukaryote transcription factors from various organisms to evaluate the discrimination energies. We find that, in accordance with our theoretical argument, binding energies nearly maximize the information gain per energy. This work suggests the adaptation of information ...

  1. Evolutionary patterns of group B Sox binding and function in Drosophila

    E-Print Network [OSTI]

    Carl, Sarah Hamilton

    2015-04-07

    ChIP-seq reads and input reads from three biological replicates in D. pseudoobscura . . . . . . . . . . . . . . . . . . . . 89 4.1 Reproducibility of biological replicate DamID samples . . . . . . . 97 4.2 Translated reads for Sox fusion proteins... ) is to bind DNA and regulate the expression of target genes; however, the complexity of combinatorial binding patterns and the sheer quantity of binding events, even in the model organism Drosophila, which has a smaller and more compact genome than hu- mans...

  2. PUMPING UP THE [N I] NEBULAR LINES

    SciTech Connect (OSTI)

    Ferland, G. J.; Porter, R. L. [Department of Physics, University of Kentucky, Lexington, KY 40506 (United States); Henney, W. J. [Centro de Radioastronomia y Astrofisica, UNAM Campus Morelia, Apartado Postal 3-72, 58090 Morelia, Michoacan (Mexico); O'Dell, C. R. [Department of Physics and Astronomy, Vanderbilt University, Box 1807-B, Nashville, TN 37235 (United States); Van Hoof, P. A. M. [Royal Observatory of Belgium, Ringlaan 3, 1180 Brussels (Belgium); Williams, R. J. R. [AWE plc, Aldermaston, Reading RG7 4PR (United Kingdom)

    2012-09-20

    The optical [N I] doublet near 5200 A is anomalously strong in a variety of emission-line objects. We compute a detailed photoionization model and use it to show that pumping by far-ultraviolet (FUV) stellar radiation previously posited as a general explanation applies to the Orion Nebula (M42) and its companion M43; but, it is unlikely to explain planetary nebulae and supernova remnants. Our models establish that the observed nearly constant equivalent width of [N I] with respect to the dust-scattered stellar continuum depends primarily on three factors: the FUV to visual-band flux ratio of the stellar population, the optical properties of the dust, and the line broadening where the pumping occurs. In contrast, the intensity ratio [N I]/H{beta} depends primarily on the FUV to extreme-ultraviolet ratio, which varies strongly with the spectral type of the exciting star. This is consistent with the observed difference of a factor of five between M42 and M43, which are excited by an O7 and B0.5 star, respectively. We derive a non-thermal broadening of order 5 km s{sup -1} for the [N I] pumping zone and show that the broadening mechanism must be different from the large-scale turbulent motions that have been suggested to explain the line widths in this H II region. A mechanism is required that operates at scales of a few astronomical units, which may be driven by thermal instabilities of neutral gas in the range 1000-3000 K. In an Appendix A, we describe how collisional and radiative processes are treated in the detailed model N I atom now included in the CLOUDY plasma code.

  3. On Line Power Plant Performance Monitoring 

    E-Print Network [OSTI]

    Ahner, D. J.; Priestley, R. R.

    1990-01-01

    PERFORMANCE MONITORING DAVID J. AHNER Manager, Power PrOduction Engineering Power Technologies, Inc. Schenectady, NY ABSTRACT Maintaining efficient and reliable plant operation is a prime objective in the generation of power. These are important... stream_source_info ESL-IE-90-06-24.pdf.txt stream_content_type text/plain stream_size 30080 Content-Encoding ISO-8859-1 stream_name ESL-IE-90-06-24.pdf.txt Content-Type text/plain; charset=ISO-8859-1 ON LINE POWER PLANT...

  4. Fluid properties determine flow line blockage potential

    SciTech Connect (OSTI)

    Hunt, A.

    1996-07-15

    A thorough understanding of fluid properties helps in determining the potential of hydrates, paraffins, or asphaltenes to block subsea flow lines. Thermal, chemical, and mechanical methods are the main ways for preventing deposition. Already in both the North Sea and the Gulf of Mexico, blockages have led to significant losses in production and reserves recovery. This first article in a two-part series discusses thermal and chemical methods in overcoming fluid behavior problems caused by hydrate and other fluid constituents in subsea multiphase flow. The paper discusses subsea production, possible problems, nucleation, growth, deposition, preventing deposition, hydrate predictions, multiphase flow, and hydrate inhibition.

  5. CXD 4601, Line Yard Fence Project (4601)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 OutreachProductswsicloudwsiclouddenDVA N C E D B LReports from the CloudGEGR-N Goods POCTBT |CUD-55 /AllenLine

  6. Plains & Eastern Clean Line HVDC Scheme

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of Natural GasAdjustmentsShirleyEnergyTher i nAandSummary Areas ofEnergy OnPeter B.EnergyInPilgrim6, 2015Line HVDC

  7. Gateway West Transmission Line | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QAsource History View New PagesSustainable UrbanKentucky: Energy ResourcesMaui Area (DOEMauiWest Transmission Line

  8. Line Extension Analysis | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIX E LISTStar2-0057-EAInvervarLeeds, UnitedLiberty PowerLine Extension Analysis Jump to:

  9. Grand Coulee Transmission Line | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIXsource History View New Pages RecentPlantMagma EnergyGoogle lendsCoulee Transmission Line

  10. LINE Commission Visits Idaho National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration would likeUniverseIMPACTThousand CubicResource and JobLCLS Operating3ledp/ The4, LINE Commission

  11. On Line Journals - Links - Cyclotron Institute

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home Room NewsInformationJesseworkSURVEY UNIVERSEHowScientificOmbuds Office LocationOn-Line Journals Acta

  12. Sequestering Uranium from Seawater: Binding Strength and Modes of Uranyl Complexes with Glutarimidedioxime

    E-Print Network [OSTI]

    Tian, Guoxin

    2013-01-01

    binding strength and modes of uranyl complexes withand the coordination modes in the uranyl glutarimidedioximeof the bonding orbitals of uranyl (? u , ? g , ? u and ? g )

  13. Binding kinetics of membrane-anchored receptors and ligands: molecular dynamics simulations and theory

    E-Print Network [OSTI]

    Jinglei Hu; Guang-Kui Xu; Reinhard Lipowsky; Thomas R. Weikl

    2015-11-24

    The adhesion of biological membranes is mediated by the binding of membrane-anchored receptor and ligand proteins. Central questions are how the binding kinetics of these proteins is affected by the membranes and by the membrane anchoring of the proteins. In this article, we (i) present detailed data for the binding of membrane-anchored proteins from coarse-grained molecular dynamics simulations, and (ii) provide a theory that describes how the binding kinetics depends on the average separation and thermal roughness of the adhering membranes, and on the anchoring, lengths, and length variations of the proteins. An important element of our theory is the tilt of bound receptor-ligand complexes and transition-state complexes relative to the membrane normals. This tilt results from an interplay of the anchoring energy and rotational entropy of the complexes and facilitates the formation of receptor-ligand bonds at membrane separations smaller than the preferred separation for binding. In our simulations, we have considered both lipid-anchored and transmembrane receptor and ligand proteins. We find that the binding equilibrium constant and binding on-rate constant of lipid-anchored proteins are considerably smaller than the binding constant and on-rate constant of rigid transmembrane proteins with identical binding domains.

  14. Energy landscapes for protein folding, binding, and aggregation : simple funnels and beyond

    E-Print Network [OSTI]

    Cho, Samuel Sung-Il

    2007-01-01

    coordinates capture protein folding on smooth landscapes.in the Prediction of Protein Folding Kinetics. Proc. Natl.Landscapes for Protein Folding, Binding, and Aggregation:

  15. Binding Energies of the Alpha Particle and the A=3 Isobars from a Theoretical Geometric Model

    E-Print Network [OSTI]

    Gustavo R. Gonzalez-Martin

    2008-05-03

    We assume a triple geometric structure for the electromagnetic nuclear interaction. This nuclear electromagnetism is used to calculate the binding energies of the alpha particle and the A=3 isobar nuclides. The approximation for the resultant wave equation which lead to the deuteron binding energy from the modified Mathieu equation for the radial eigenvalue equation also establishes proton-electron-proton magnetic bonds in these nuclides and determines their binding energies. Completely theoretical calculations give 28.5 Mev., 7.64 Mev. and 8.42 Mev. for the binding energies of the alpha particle, the helium 3 isotope and tritium respectively. These values admit correction factors due to the approximations made.

  16. Sequestering Uranium from Seawater: Binding Strength and Modes of Uranyl Complexes with Glutarimidedioxime

    E-Print Network [OSTI]

    Tian, Guoxin

    2013-01-01

    data_request/cif. OECD, Uranium 2009: Resources, Productionthermodynamics of uranium”, (H. Wanner and I. Forest,of California. Sequestering uranium from seawater: binding

  17. EIS-0421: Big Eddy-Knight Transmission Line

    Broader source: Energy.gov [DOE]

    BPA is proposing to build a new 500 kilovolt (kV) transmission line in Wasco County, Oregon and Klickitat County, Washington and a new substation in Klickitat County. The new BPA transmission line...

  18. Emailed Comments of Elizabeth Chimento RE: Pepco Scheduled Line...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Scheduled Line Repair Dec. 1-20, 2006 Docket No. EO-05-01. Regarding the planned line outage, scheduled for Feb. 20 through Mar. 6, I am requesting that the residents near the...

  19. Radio-Frequency Inverters With Transmission-Line Input Networks

    E-Print Network [OSTI]

    Phinney, Joshua W.

    A soft-switching inverter topology (the Class Phi ) is presented which draws dc source current through a transmission line or a lumped-network approximation of a distributed line. By aligning the inverter switching frequency ...

  20. Real Time Pricing Tie Line Control: A Case Study 

    E-Print Network [OSTI]

    Childress, R.

    1999-01-01

    A Tie Line Control System maintains the purchase quantity of electrical energy an industrial facility buys from a utility company. Implementing an advanced Tie Line Control system automates the buy versus make decision process for an industrial...

  1. Frenkel Line and Solubility Maximum in Supercritical Fluids

    E-Print Network [OSTI]

    C. Yang; V. V. Brazhkin; M. T. Dove; K. Trachenko

    2015-02-27

    A new dynamic line, the Frenkel line, has recently been proposed to separate the supercritical state into rigid-liquid and non-rigid gas-like uid. The location of Frenkel line on the phase diagram is unknown for real uids. Here, we map the Frenkel line for three important systems: CO2, H2O and CH4. This provides an important demarcation on the phase diagram of these systems, the demarcation that separates two distinct physical states with liquid-like and gas-like properties. We ?nd that the Frenkel line can have similar trend as the melting line above the critical pressure. Moreover, we discuss the relationship between unexplained solubility maxima and Frenkel line, and propose that the Frenkel line corresponds to the optimal conditions for solubility.

  2. Mode conversation losses in overmolded millimeter wave transmission lines

    E-Print Network [OSTI]

    Tax, David S. (David Samuel)

    2008-01-01

    Millimeter wave transmission lines are integral components for many important applications like nuclear fusion and NMR spectroscopy. In low loss corrugated transmission lines propagating the HE,1 mode with a high waveguide ...

  3. An on-line human signature verification system 

    E-Print Network [OSTI]

    Simsek, Burc A

    2000-01-01

    A study on human signature verification is presented. A literature survey of current and past methodologies for on-line signature verification is followed by a new approach to the problem of on-line signature verification. ...

  4. Secretary Chu Thanks Utility Staff and Line Workers For Their...

    Energy Savers [EERE]

    Utility Staff and Line Workers For Their Efforts to Restore Power in the Wake of Hurricane Sandy Secretary Chu Thanks Utility Staff and Line Workers For Their Efforts to...

  5. ExoMol line lists VIII: A variationally computed line list for hot formaldehyde

    E-Print Network [OSTI]

    Al-Refaie, Ahmed F; Tennyson, Jonathan; Yurchenko, Sergei N

    2015-01-01

    A computed line list for formaldehyde, H$_2{}^{12}$C$^{16}$O, applicable to temperatures up to $T=1500$~K is presented. An empirical potential energy and {\\it ab initio} dipole moment surfaces are used as the input to nuclear motion program TROVE. The resulting line list, referred to as \\textit{AYTY}, contains 10.3 million rotational-vibrational states and around 10 billion transition frequencies. Each transition includes associated Einstein-$A$ coefficients and absolute transition intensities, for wavenumbers below 10~000 cm\\(^{-1}\\) and rotational excitations up to \\(J=70\\). Room-temperature spectra are compared with laboratory measurements and data currently available in the HITRAN database. These spectra show excellent agreement with experimental spectra and highlight the gaps and limitations of the HITRAN data. The full line list is available from the CDS database as well as at \\url{www.exomol.com}.

  6. SunLine Tests HHICE Bus in Desert Climate

    SciTech Connect (OSTI)

    Not Available

    2006-10-01

    Fact sheet describes the demonstration of a hybrid hydrogen internal combustion engine (HHICE) bus at SunLine Transit Agency.

  7. Mapping small molecule binding data to structural domains

    E-Print Network [OSTI]

    Kruger, Felix A.; Rostom, Raghd; Overington, John P.

    2012-12-07

    R_lig-bind 25 128 P40238 fn3 391 475 P48443 Nuc_recep-AF1 24 134 P48443 Hormone_recep 248 443 P48443 zf-C4 137 206 P54219 MFS_1 77 433 P54219 MFS_1 422 493 Q92813 T4_deiodinase 4 262 P08506 PBP5_C 285 376 P08506 Peptidase_S11 28 266 P10826 Hormone_recep 222 406... P10826 zf-C4 86 155 P10828 Hormone_recep 257 445 P10828 zf-C4 105 176 P11926 Orn_Arg_deC_N 44 282 P11926 Orn_DAP_Arg_deC 285 408 P19793 Nuc_recep-AF1 16 127 P19793 Hormone_recep 251 442 P19793 zf-C4 133 202 P27815 PDEase_I 432 676 P28702 Hormone...

  8. On the nuclear interaction. Potential, binding energy and fusion reaction

    E-Print Network [OSTI]

    I. Casinos

    2008-05-22

    The nuclear interaction is responsible for keeping neutrons and protons joined in an atomic nucleus. Phenomenological nuclear potentials, fitted to experimental data, allow one to know about the nuclear behaviour with more or less success where quantum mechanics is hard to be used. A nuclear potential is suggested and an expression for the potential energy of two nuclear entities, either nuclei or nucleons, is developed. In order to estimate parameters in this expression, some nucleon additions to nuclei are considered and a model is suggested as a guide of the addition process. Coulomb barrier and energy for the addition of a proton to each one of several nuclei are estimated by taking into account both the nuclear and electrostatic components of energy. Studies on the binding energies of several nuclei and on the fusion reaction of two nuclei are carried out.

  9. Quasielastic electron-deuteron scattering in the weak binding approximation

    SciTech Connect (OSTI)

    Ethier, Jacob J. [William and Mary College, JLAB; Doshi, Nidhi P. [Carnegie Mellon University; Malace, Simona P. [JLAB; Melnitchouk, Wally [JLAB

    2014-06-01

    We perform a global analysis of all available electron-deuteron quasielastic scattering data using Q^2-dependent smearing functions that describe inclusive inelastic e-d scattering within the weak binding approximation. We study the dependence of the cross sections on the deuteron wave function and the off-shell extrapolation of the elastic electron-nucleon cross section, which show particular sensitivity at x >> 1. The excellent overall agreement with data over a large range of Q^2 and x suggest a limited need for effects beyond the impulse approximation, with the exception of the very high-x or very low-Q^2 regions, where short-distance effects in the deuteron become more relevant.

  10. Control of stochasticity in magnetic field lines

    E-Print Network [OSTI]

    Cristel Chandre; Michel Vittot; Guido Ciraolo; Philippe Ghendrih; Ricardo Lima

    2005-11-03

    We present a method of control which is able to create barriers to magnetic field line diffusion by a small modification of the magnetic perturbation. This method of control is based on a localized control of chaos in Hamiltonian systems. The aim is to modify the perturbation locally by a small control term which creates invariant tori acting as barriers to diffusion for Hamiltonian systems with two degrees of freedom. The location of the invariant torus is enforced in the vicinity of the chosen target. Given the importance of confinement in magnetic fusion devices, the method is applied to two examples with a loss of magnetic confinement. In the case of locked tearing modes, an invariant torus can be restored that aims at showing the current quench and therefore the generation of runaway electrons. In the second case, the method is applied to the control of stochastic boundaries allowing one to define a transport barrier within the stochastic boundary and therefore to monitor the volume of closed field lines.

  11. On-line subsea multiphase flow measurement

    SciTech Connect (OSTI)

    High, G.; Frantzen, K.H.; Marshall, M.

    1995-12-31

    This paper describes the final detailed design, engineering, and installation phase of a Joint Industry Program to qualify a robust subsea multiphase flowmeter module for long-term installation on a North Sea manifold tie-in. Multiphase subsea production has become a common method of hydrocarbon recovery in all areas of offshore E and P. In the North Sea, many developments are subsea satellites with multiphase well-fluids being comingled prior to processing. The system described meets this challenge by offering a cost effective solution to real-time well monitoring as an alternative to the conventional test separator, removing the need for test lines and shutting in wells for testing. The multiphase instrument allows on-line well fluid analysis, and is also an important tool for reservoir management and field analysis, and provides a means of implementing field allocation metering thereby simplifying small marginal field developments. This project is one of the first subsea multiphase flowmeter installations engineered for long-term subsea service, and designed as an integrated component of the subsea production control system.

  12. Supplemental Material Generalized Streak Lines Advected Tangent Curves

    E-Print Network [OSTI]

    of advected tangent curves. It turns out that generalized streak lines [WTS+ 07] can be described using. Generalized Streak Lines w = v, ¯f = FFF Wiebel et al. [WTS+ 07] introduced a generalization of streak lines. In Data Visualization 2003. Proc. VisSym 03, pages 141­148, 2003. [WTS+ 07] Alexander Wiebel, Xavier

  13. Synthesis of lumped transmission-line analogs Joshua W. Phinney

    E-Print Network [OSTI]

    Perreault, Dave

    of transmission lines to power electronics is a promising technique for miniaturizing dc-dc and dc- ac convertersSynthesis of lumped transmission-line analogs Joshua W. Phinney Exponent, Inc. 420 Lexington Ave-- Transmission lines and their lumped approximating networks have long been incorporated into radio

  14. INNOVATIVE CONCEPTS FOR ON-LINE SYNCHRONOUS GENERATOR PARAMETER ESTIMATION

    E-Print Network [OSTI]

    INNOVATIVE CONCEPTS FOR ON-LINE SYNCHRONOUS GENERATOR PARAMETER ESTIMATION by Elias Kyriakides FOR ON-LINE SYNCHRONOUS GENERATOR PARAMETER ESTIMATION by Elias Kyriakides has been approved December ABSTRACT A method to identify synchronous generator parameters from on-line measurements is presented

  15. The spectra of super line multigraphs Department of Computer Science

    E-Print Network [OSTI]

    Ellis, Robert B.

    The spectra of super line multigraphs Jay Bagga Department of Computer Science Ball State, the super line multigraph of index r of G, denoted Mr(G), has for vertices all the r-subsets of edges. Two all the eigenvalues of Mr(G) and their multiplicities in terms of those of G. Key words. super line

  16. Software Product Lines: a Case Study Mark Ardis Nigel Daleyy

    E-Print Network [OSTI]

    Ardis, Mark

    , design patterns, and automated testing. The case study is based on Header Table, an example product lineSoftware Product Lines: a Case Study Mark Ardis Nigel Daleyy Daniel Ho manz Harvey Siyx David Weiss{ January 28, 2000 Abstract A software product line is a family of products that share common features

  17. Development of Gamma-Emitting Receptor Binding Radiopharmace

    SciTech Connect (OSTI)

    Reba, Richard

    2003-02-20

    The long-term objective is to develop blood-brain barrier (BBB) permeable m2-selective (relative to m1, m3, and m4) receptor-binding radiotracers and utilize these radiotracers for quantifying receptor concentrations obtained from PET or SPECT images of human brain. In initial studies, we concluded that the lipophilicity and high affinity prevented (R,S)-I-QNB from reaching a flow-independent and receptor-dependent state in a reasonable time. Thus, it was clear that (R,S)-I-QNB should be modified. Therefore, during the last portion of this funded research, we proposed that more polar heterocycles should help accomplish that. Since reports of others concluded that radiobromination and radiofluorination of the unactivated phenyl ring is not feasible (Newkome et al,,1982), we, therefore, explored during this grant period a series of analogues of (R)-QNB in which one or both of the six-membered phenyl rings is replaced by a five-membered thienyl (Boulay et al., 1995), or furyl ring. The chemistry specific aims were to synthesize novel compounds designed to be m2-selective mAChR ligands capable of penetrating into the CNS, and develop methods for efficient radiolabeling of promising m2-selective muscarinic ligands. The pharmacology specific aims were to determine the affinity and subtype-selectivity of the novel compounds using competition binding studies with membranes from cells that express each of the five muscarinic receptor subtypes, to determine the ability of the promising non-radioactive compounds and radiolabeled novel compounds to cross the BBB, to determine the biodistribution, in-vivo pharmacokinetics, and in-vitm kinetics of promising m2-selective radioligands and to determine the distribution of receptors for the novel m2-selective radioligands using quantitative autoradiography of rat brain, and compare this distribution to the distribution of known m2-selective compounds.

  18. Comparing the performace of f1 testers versus their inbred line parents in evaluating experimental sorghum b and r lines in testcrosses. 

    E-Print Network [OSTI]

    Packer, Daniel Jacob

    2009-05-15

    versus inbred line testers for evaluating experimental sorghum lines in testcrosses Line x tester analyses were performed to assess tester consistency in assigning ranks. With one exception, all of the line x tester analyses were non...

  19. Single-Molecule Dynamics Reveals Cooperative Binding-Folding in Protein Recognition

    SciTech Connect (OSTI)

    Wang, Jin; Lu, Qiang N.; Lu, H PETER.

    2006-07-01

    The study of associations between two biomolecules is the key to understand molecular recognition and function. Molecular function is often thought to be determined by the underlying structures. Here, combining single molecule study of protein binding with an energy landscape inspired microscopic model, we found strong evidences that bio-molecular recognition is determined by flexibilities in addition to structures. Our model is based on coarse grained molecular dynamics performed on the residue level with the energy function biased towards the native binding structure (Go model). With our model, the underlying free energy landscape of the binding can be explored. Two distinct conformational states as free energy minimum, one with partially folding of CBD and significant binding of CBD to CDC42, and another with native folding of CBD and native binding of CBD to CDC42, are clearly seen. This shows the binding process proceeds with significant interface binding of CBD with CDC42 first without complete folding of CBD. Finally binding and folding are coupled with each other cooperatively to reach the native binding state. The single molecule experimental finding of the dynamic fluctuations between the loosely bound and closely bound conformational states can be identified with theoretically calculated free energy minimum and quantitatively explained in our model as a result of binding associated with large conformational changes. Theoretical predictions have identified certain key residues for binding which are consistent with mutational experiments. The combined study provides a test ground for fundamental mechanisms as well as insights into design and further explorations on biomolecular recognition with large conformational changes.

  20. Discriminating binding mechanisms of an intrinsically disordered protein via a multi-state coarse-grained model

    SciTech Connect (OSTI)

    Knott, Michael [Department of Chemistry, Cambridge University, Lensfield Road, Cambridge CB2 1EW (United Kingdom)] [Department of Chemistry, Cambridge University, Lensfield Road, Cambridge CB2 1EW (United Kingdom); Best, Robert B., E-mail: robertbe@helix.nih.gov [Department of Chemistry, Cambridge University, Lensfield Road, Cambridge CB2 1EW (United Kingdom); Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892-0520 (United States)

    2014-05-07

    Many proteins undergo a conformational transition upon binding to their cognate binding partner, with intrinsically disordered proteins (IDPs) providing an extreme example in which a folding transition occurs. However, it is often not clear whether this occurs via an “induced fit” or “conformational selection” mechanism, or via some intermediate scenario. In the first case, transient encounters with the binding partner favour transitions to the bound structure before the two proteins dissociate, while in the second the bound structure must be selected from a subset of unbound structures which are in the correct state for binding, because transient encounters of the incorrect conformation with the binding partner are most likely to result in dissociation. A particularly interesting situation involves those intrinsically disordered proteins which can bind to different binding partners in different conformations. We have devised a multi-state coarse-grained simulation model which is able to capture the binding of IDPs in alternate conformations, and by applying it to the binding of nuclear coactivator binding domain (NCBD) to either ACTR or IRF-3 we are able to determine the binding mechanism. By all measures, the binding of NCBD to either binding partner appears to occur via an induced fit mechanism. Nonetheless, we also show how a scenario closer to conformational selection could arise by choosing an alternative non-binding structure for NCBD.

  1. Variety of lines Main theorem Kummer coverings Precise statement Proof Singularities Lines in Fermat hypersurface and M0,n

    E-Print Network [OSTI]

    Terasoma, Tomohide

    . . . . . . Variety of lines Main theorem Kummer coverings Precise statement Proof Singularities://gauss.ms.u-tokyo.ac.jp Arrangements of Hyperplanes, in Hokkaido University, 13th Aug 2009 #12;. . . . . . Variety of lines Main theorem Kummer coverings Precise statement Proof Singularities Contents . ..1 Variety of lines . ..2 Main

  2. Comparison of on-line and off-line methods to quantify reactive oxygen species (ROS) in atmospheric aerosols

    E-Print Network [OSTI]

    Fuller, S. J.; Wragg, F. P. H.; Nutter, J.; Kalberer, M.

    2014-04-08

      a   wide   range   of   inorganic   and   organic  72   compounds   such   as   transition  metals,   hydrogen  peroxide   (H2O2),   radicals   (e.g.,  73   OH??,  O2???),  and  organic  (hydro...   H2O2  were  used  to  optimize  operation  conditions  of  the  on-­?line  instrument  and  276   to   compare   the   performance   of   on-­?line   and   off-­?line   quantification   methods.  277...

  3. On-Line Digit Recognition using Off-Line Features A.Teredesai

    E-Print Network [OSTI]

    Fernandez, Thomas

    @cedar.buffalo.edu Abstract This paper describes a classification method for on-line handwritten digits based on off. This framework for handwritten digit classification is based on genetic programming (GP). Several issues in pre effective for digit classification and pairwise discrimination of confusing allographs [9] and b) Genetic

  4. Method for bonding a transmission line to a downhole tool

    DOE Patents [OSTI]

    Hall, David R. (Provo, UT); Fox, Joe (Spanish Fork, UT)

    2007-11-06

    An apparatus for bonding a transmission line to the central bore of a downhole tool includes a pre-formed interface for bonding a transmission line to the inside diameter of a downhole tool. The pre-formed interface includes a first surface that substantially conforms to the outside contour of a transmission line and a second surface that substantially conforms to the inside diameter of a downhole tool. In another aspect of the invention, a method for bonding a transmission line to the inside diameter of a downhole tool includes positioning a transmission line near the inside wall of a downhole tool and placing a mold near the transmission line and the inside wall. The method further includes injecting a bonding material into the mold and curing the bonding material such that the bonding material bonds the transmission line to the inside wall.

  5. Calibrating transport lines using LOCO techniques

    SciTech Connect (OSTI)

    Yves Roblin

    2011-09-01

    With the 12GeV upgrade underway at CEBAF, there is a need to re-characterize the beamlines after the modifications made to it to accommodate running at higher energies. We present a linear perturbation approach to calibrating the optics model of transport lines. This method is adapted from the LOCO method in use for storage rings. We consider the effect of quadrupole errors, dipole construction errors as well as beam position monitors and correctors calibrations. The ideal model is expanded to first order in Taylor series of the quadrupole errors. A set of difference orbits obtained by exciting the correctors along the beamline is taken, yielding the measured response matrix. An iterative procedure is invoked and the quadrupole errors as well as beam position monitors and corrector calibration factors are obtained. Here we present details of the method and results of first measurements at CEBAF in early 2011.

  6. Nonlinear transmission line based electron beam driver

    SciTech Connect (OSTI)

    French, David M.; Hoff, Brad W.; Tang Wilkin; Heidger, Susan; Shiffler, Don [Directed Energy Directorate, Air Force Research Laboratory, Kirtland AFB, New Mexico 87117 (United States); Allen-Flowers, Jordan [Program in Applied Mathematics, University of Arizona, Tucson, Arizona 85721 (United States)

    2012-12-15

    Gated field emission cathodes can provide short electron pulses without the requirement of laser systems or cathode heating required by photoemission or thermionic cathodes. The large electric field requirement for field emission to take place can be achieved by using a high aspect ratio cathode with a large field enhancement factor which reduces the voltage requirement for emission. In this paper, a cathode gate driver based on the output pulse train from a nonlinear transmission line is experimentally demonstrated. The application of the pulse train to a tufted carbon fiber field emission cathode generates short electron pulses. The pulses are approximately 2 ns in duration with emission currents of several mA, and the train contains up to 6 pulses at a frequency of 100 MHz. Particle-in-cell simulation is used to predict the characteristic of the current pulse train generated from a single carbon fiber field emission cathode using the same technique.

  7. Dispersion-free radial transmission lines

    DOE Patents [OSTI]

    Caporaso, George J. (Livermore, CA); Nelson, Scott D. (Patterson, CA)

    2011-04-12

    A dispersion-free radial transmission line ("DFRTL") preferably for linear accelerators, having two plane conductors each with a central hole, and an electromagnetically permeable material ("EPM") between the two conductors and surrounding a channel connecting the two holes. At least one of the material parameters of relative magnetic permeability, relative dielectric permittivity, and axial width of the EPM is varied as a function of radius, so that the characteristic impedance of the DFRTL is held substantially constant, and pulse transmission therethrough is substantially dispersion-free. Preferably, the EPM is divided into concentric radial sections, with the varied material parameters held constant in each respective section but stepwise varied between sections as a step function of the radius. The radial widths of the concentric sections are selected so that pulse traversal time across each section is the same, and the varied material parameters of the concentric sections are selected to minimize traversal error.

  8. On-line tritium production monitor

    DOE Patents [OSTI]

    Mihalczo, John T. (Oak Ridge, TN)

    1993-01-01

    A scintillation optical fiber system for the on-line monitoring of nuclear reactions in an event-by-event manner is described. In the measurement of tritium production one or more optical fibers are coated with enriched .sup.6 Li and connected to standard scintillation counter circuitry. A neutron generated .sup.6 Li(n )T reaction occurs in the coated surface of .sup.6 Li-coated fiber to produce energetic alpha and triton particles one of which enters the optical fiber and scintillates light through the fiber to the counting circuit. The coated optical fibers can be provided with position sensitivity by placing a mirror at the free end of the fibers or by using pulse counting circuits at both ends of the fibers.

  9. On-line tritium production monitor

    DOE Patents [OSTI]

    Mihalczo, J.T.

    1993-11-23

    A scintillation optical fiber system for the on-line monitoring of nuclear reactions in an event-by-event manner is described. In the measurement of tritium production one or more optical fibers are coated with enriched {sup 6}Li and connected to standard scintillation counter circuitry. A neutron generated {sup 6}Li(n)T reaction occurs in the coated surface of {sup 6}Li-coated fiber to produce energetic alpha and triton particles one of which enters the optical fiber and scintillates light through the fiber to the counting circuit. The coated optical fibers can be provided with position sensitivity by placing a mirror at the free end of the fibers or by using pulse counting circuits at both ends of the fibers. 5 figures.

  10. Atomic line emission analyzer for hydrogen isotopes

    DOE Patents [OSTI]

    Kronberg, James W. (108 Independent Blvd., Aiken, SC 29801)

    1993-01-01

    Apparatus for isotopic analysis of hydrogen comprises a low pressure chamber into which a sample of hydrogen is introduced and then exposed to an electrical discharge to excite the electrons of the hydrogen atoms to higher energy states and thereby cause the emission of light on the return to lower energy states, a Fresnel prism made at least in part of a material anomalously dispersive to the wavelengths of interest for dispersing the emitted light, and a photodiode array for receiving the dispersed light. The light emitted by the sample is filtered to pass only the desired wavelengths, such as one of the lines of the Balmer series for hydrogen, the wavelengths of which differ slightly from one isotope to another. The output of the photodiode array is processed to determine the relative amounts of each isotope present in the sample. Additionally, the sample itself may be recovered using a metal hydride.

  11. Atomic line emission analyzer for hydrogen isotopes

    DOE Patents [OSTI]

    Kronberg, J.W.

    1993-03-30

    Apparatus for isotopic analysis of hydrogen comprises a low pressure chamber into which a sample of hydrogen is introduced and then exposed to an electrical discharge to excite the electrons of the hydrogen atoms to higher energy states and thereby cause the emission of light on the return to lower energy states, a Fresnel prism made at least in part of a material anomalously dispersive to the wavelengths of interest for dispersing the emitted light, and a photodiode array for receiving the dispersed light. The light emitted by the sample is filtered to pass only the desired wavelengths, such as one of the lines of the Balmer series for hydrogen, the wavelengths of which differ slightly from one isotope to another. The output of the photodiode array is processed to determine the relative amounts of each isotope present in the sample. Additionally, the sample itself may be recovered using a metal hydride.

  12. Atomic line emission analyzer for hydrogen isotopes

    DOE Patents [OSTI]

    Kronberg, J.W.

    1991-05-08

    Apparatus for isotopic analysis of hydrogen comprises a low pressure chamber into which a sample of hydrogen is introduced and then exposed to an electrical discharge to excite the electrons of the hydrogen atoms to higher energy states and thereby cause the emission of light on the return to lower energy states, a Fresnel prism made at least in part of a material anomalously dispersive to the wavelengths of interest for dispersing the emitted light, and a photodiode array for receiving the dispersed light. The light emitted by the sample is filtered to pass only the desired wavelengths, such as one of the lines of the Balmer series for hydrogen, the wavelengths of which differ slightly from one isotope to another. The output of the photodiode array is processed to determine the relative amounts of each isotope present in the sample. Additionally, the sample itself may be recovered using, a metal hydride.

  13. Second Line of Defense Spares Program Assessment

    SciTech Connect (OSTI)

    Henderson, Dale L.; Muller, George; Mercier, Theresa M.; Brigantic, Robert T.; Perkins, Casey J.; Cooley, Scott K.

    2012-11-20

    The Office of the Second Line of Defense (SLD) is part of the Department of Energy‘s (DOE) National Nuclear Security Administration (NNSA). The SLD Program accomplishes its critical global security mission by forming cooperative relationships with partner countries to install passive radiation detection systems that augment traditional inspection and law enforcement measures by alerting border officials to the presence of special nuclear or other radiological materials in cross-border traffic. An important tenet of the program is to work collaboratively with these countries to establish the necessary processes, procedures, infrastructure and conditions that will enable them to fully assume the financial and technical responsibilities for operating the equipment. As the number of operational deployments grows, the SLD Program faces an increasingly complex logistics process to promote the timely and efficient supply of spare parts.

  14. Moving contact line of a volatile fluid

    E-Print Network [OSTI]

    V. Janecek; B. Andreotti; D. Prazak; T. Barta; V. S. Nikolayev

    2012-12-15

    Interfacial flows close to a moving contact line are inherently multi-scale. The shape of the interface and the flow at meso- and macroscopic scales inherit an apparent interface slope and a regularization length, both called after Voinov, from the dynamical processes at work at the microscopic level. Here, we solve this inner problem in the case of a volatile fluid at equilibrium with its vapor. The evaporative/condensation flux is then controlled by the dependence of the saturation temperature on interface curvature -- the so-called Kelvin effect. We derive the dependencies of the Voinov angle and of the Voinov length as functions of the substrate temperature. The relevance of the predictions for experimental problems is finally discussed.

  15. Moving contact line of a volatile fluid

    E-Print Network [OSTI]

    Janecek, V; Prazak, D; Barta, T; Nikolayev, V S

    2012-01-01

    Interfacial flows close to a moving contact line are inherently multi-scale. The shape of the interface and the flow at meso- and macroscopic scales inherit an apparent interface slope and a regularization length, both called after Voinov, from the dynamical processes at work at the microscopic level. Here, we solve this inner problem in the case of a volatile fluid at equilibrium with its vapor. The evaporative/condensation flux is then controlled by the dependence of the saturation temperature on interface curvature -- the so-called Kelvin effect. We derive the dependencies of the Voinov angle and of the Voinov length as functions of the substrate temperature. The relevance of the predictions for experimental problems is finally discussed.

  16. USING DNASE DIGESTION DATA TO ACCURATELY IDENTIFY TRANSCRIPTION FACTOR BINDING SITES

    E-Print Network [OSTI]

    Hartemink, Alexander

    USING DNASE DIGESTION DATA TO ACCURATELY IDENTIFY TRANSCRIPTION FACTOR BINDING SITES KAIXUAN LUO1. But methods combining DNase digestion data with TF binding specificity information could potentially be used on the genomic digestion prod- ucts of deoxyribonuclease I (DNase I, which we will simply call DNase) might

  17. ContextSensitive Binding by the Laminar Circuits of V1 and V2

    E-Print Network [OSTI]

    Grossberg, Stephen

    Context­Sensitive Binding by the Laminar Circuits of V1 and V2: Unified Model Perceptual Grouping is presented how laminar circuits visual cortical areas implement context­sensitive binding processes such perceptual grouping and attention. model proposes how specific laminar circuits allow responses visual

  18. Kinetics of Bile Salt Binding to Liposomes Revealed by Carboxyfluorescein Release and

    E-Print Network [OSTI]

    Hinow, Peter

    Kinetics of Bile Salt Binding to Liposomes Revealed by Carboxyfluorescein Release and Mathematical by the binding of different bile salts to the leaflets of the lipid bilayer. We find that the permeability of the liposomal bilayer depends on the difference in the concentrations of bile salt in the inner and outer

  19. Contributions to the Binding Free Energy of Ligands to Avidin and Streptavidin

    E-Print Network [OSTI]

    Lazaridis, Themis

    of New York, New York, New York ABSTRACT The free energy of binding of a ligand to a macromoleculeContributions to the Binding Free Energy of Ligands to Avidin and Streptavidin Themis Lazaridis is here formally decom- posed into the (effective) energy of interaction, reorganization energy

  20. Using Multiconformation Continuum Electrostatics to Compare Chloride Binding Motifs in -Amylase,

    E-Print Network [OSTI]

    Gunner, Marilyn

    Using Multiconformation Continuum Electrostatics to Compare Chloride Binding Motifs in -Amylase electrostatics (MCCE), we show that the changes of chloride binding to -amylase, human serum albumin (HSA) and Omp32 with pH, and of -amylase with mutation agree well with experimental data. The three proteins

  1. Retention of Conformational Entropy upon Calmodulin Binding to Target Peptides is Driven by Transient Salt Bridges

    SciTech Connect (OSTI)

    Smith, Dayle MA; Straatsma, TP; Squier, Thomas C.

    2012-10-03

    Calmodulin (CaM) is a highly flexible calcium-binding protein that mediates signal transduction through an ability to differentially bind to highly variable binding sequences in target proteins. To identify how binding affects CaM motions, and its relationship to conformational entropy and target peptide sequence, we have employed fully atomistic, explicit solvent molecular dynamics simulations of unbound CaM and CaM bound to five different target peptides. The calculated CaM conformational binding entropies correlate with experimentally derived conformational entropies with a correlation coefficient R2 of 0.95. Selected side-chain interactions with target peptides restrain interhelical loop motions, acting to tune the conformational entropy of the bound complex via widely distributed CaM motions. In the complex with the most conformational entropy retention (CaM in complex with the neuronal nitric oxide synthase binding sequence), Lys-148 at the C-terminus of CaM forms transient salt bridges alternating between Glu side chains in the N-domain, the central linker, and the binding target. Additional analyses of CaM structures, fluctuations, and CaM-target interactions illuminate the interplay between electrostatic, side chain, and backbone properties in the ability of CaM to recognize and discriminate against targets by tuning its conformational entropy, and suggest a need to consider conformational dynamics in optimizing binding affinities.

  2. Distinct p53 genomic binding patterns in normal and cancer-derived human cells

    SciTech Connect (OSTI)

    Botcheva K.; McCorkle S. R.; McCombie W. R.; Dunn J. J.; Anderson C. W.

    2011-12-15

    We report here genome-wide analysis of the tumor suppressor p53 binding sites in normal human cells. 743 high-confidence ChIP-seq peaks representing putative genomic binding sites were identified in normal IMR90 fibroblasts using a reference chromatin sample. More than 40% were located within 2 kb of a transcription start site (TSS), a distribution similar to that documented for individually studied, functional p53 binding sites and, to date, not observed by previous p53 genome-wide studies. Nearly half of the high-confidence binding sites in the IMR90 cells reside in CpG islands, in marked contrast to sites reported in cancer-derived cells. The distinct genomic features of the IMR90 binding sites do not reflect a distinct preference for specific sequences, since the de novo developed p53 motif based on our study is similar to those reported by genome-wide studies of cancer cells. More likely, the different chromatin landscape in normal, compared with cancer-derived cells, influences p53 binding via modulating availability of the sites. We compared the IMR90 ChIPseq peaks to the recently published IMR90 methylome1 and demonstrated that they are enriched at hypomethylated DNA. Our study represents the first genome-wide, de novo mapping of p53 binding sites in normal human cells and reveals that p53 binding sites reside in distinct genomic landscapes in normal and cancer-derived human cells.

  3. Identification of Substrate Binding Sites in Enzymes by Computational Solvent Mapping

    E-Print Network [OSTI]

    Vajda, Sandor

    . Dennis, Biotechnology Research Institute, National Research Council of Canada, Montreal, Canada; L. Brown * 1 Program in Bioinformatics Boston University, Boston, MA 02215, USA 2 Department of Biomedical with the most favorable binding free energy. The method then finds the con- sensus site that binds the highest

  4. A PARLOG Implementation of Government-Binding Theory Robert J. Kuhns

    E-Print Network [OSTI]

    on the principles and constraints of Government- Binding Theory. The parser outputs a set of licensing relations~odules of tile theory. BACKGROUND Parse,'s based oil Government-Binding (GB) Theory have been tile subject\\er, they all approach parsing as a sequential process, and allhough there have been a number o[' efforts oil

  5. STAR/MPI: Binding a Parallel Library to Interactive Symbolic Algebra Systems

    E-Print Network [OSTI]

    Cooperman, Gene

    STAR/MPI: Binding a Parallel Library to Interactive Symbolic Algebra Systems Gene Cooperman 1 gene of symbolic algebra systems have felt the need for greater CPU power. Yet few of them have ventured/MPI includes a simple SPMD architecture on top of this MPI binding. An important class of sequential algo

  6. Dynamics of intracellular Ca$^{2+}$ oscillations in the presence of multisite Ca$^{2+}$-binding proteins

    E-Print Network [OSTI]

    Roberto Chignola; Alessio Del Fabbro; Edoardo Milotti

    2009-09-10

    We study the dynamics of intracellular calcium oscillations in the presence of proteins that bind calcium on multiple sites and that are generally believed to act as passive calcium buffers in cells. We find that multisite calcium-binding proteins set a sharp threshold for calcium oscillations. Even with high concentrations of calcium-binding proteins, internal noise, which shows up spontaneously in cells in the process of calcium wave formation, can lead to self-oscillations. This produces oscillatory behaviors strikingly similar to those observed in real cells. In addition, for given intracellular concentrations of both calcium and calcium-binding proteins the regularity of these oscillations changes and reaches a maximum as a function noise variance, and the overall system dynamics displays stochastic coherence. We conclude that calcium-binding proteins may have an important and active role in cellular communication.

  7. Expression, purification, crystallization and structure of human adipocyte lipid-binding protein (aP2)

    SciTech Connect (OSTI)

    Marr, Eric; Tardie, Mark; Carty, Maynard; Brown Phillips, Tracy; Wang, Ing-Kae; Soeller, Walt; Qiu, Xiayang Karam, George

    2006-11-01

    The crystal structure of human adipocyte lipid-binding protein (aP2) with a bound palmitate is reported at 1.5 Å resolution. Human adipocyte lipid-binding protein (aP2) belongs to a family of intracellular lipid-binding proteins involved in the transport and storage of lipids. Here, the crystal structure of human aP2 with a bound palmitate is described at 1.5 Å resolution. Unlike the known crystal structure of murine aP2 in complex with palmitate, this structure shows that the fatty acid is in a folded conformation and that the loop containing Phe57 acts as a lid to regulate ligand binding by excluding solvent exposure to the central binding cavity.

  8. Structural basis of substrate discrimination and integrin binding by autotaxin

    SciTech Connect (OSTI)

    Hausmann, Jens; Kamtekar, Satwik; Christodoulou, Evangelos; Day, Jacqueline E.; Wu, Tao; Fulkerson, Zachary; Albers, Harald M.H.G.; van Meeteren, Laurens A.; Houben, Anna J.S.; van Zeijl, Leonie; Jansen, Silvia; Andries, Maria; Hall, Troii; Pegg, Lyle E.; Benson, Timothy E.; Kasiem, Mobien; Harlos, Karl; Vander Kooi, Craig W.; Smyth, Susan S.; Ovaa, Huib; Bollen, Mathieu; Morris, Andrew J.; Moolenaar, Wouter H.; Perrakis, Anastassis (Pfizer); (Leuven); (Oxford); (NCI-Netherlands); (Kentucky)

    2013-09-25

    Autotaxin (ATX, also known as ectonucleotide pyrophosphatase/phosphodiesterase-2, ENPP2) is a secreted lysophospholipase D that generates the lipid mediator lysophosphatidic acid (LPA), a mitogen and chemoattractant for many cell types. ATX-LPA signaling is involved in various pathologies including tumor progression and inflammation. However, the molecular basis of substrate recognition and catalysis by ATX and the mechanism by which it interacts with target cells are unclear. Here, we present the crystal structure of ATX, alone and in complex with a small-molecule inhibitor. We have identified a hydrophobic lipid-binding pocket and mapped key residues for catalysis and selection between nucleotide and phospholipid substrates. We have shown that ATX interacts with cell-surface integrins through its N-terminal somatomedin B-like domains, using an atypical mechanism. Our results define determinants of substrate discrimination by the ENPP family, suggest how ATX promotes localized LPA signaling and suggest new approaches for targeting ATX with small-molecule therapeutic agents.

  9. Donor binding energy and thermally activated persistent photoconductivity in high mobility ,,001... AlAs quantum wells

    E-Print Network [OSTI]

    Grayson, Matthew

    material parameters such as the donor binding energy and doping efficiency have been obscured by substrateDonor binding energy and thermally activated persistent photoconductivity in high mobility ,,001. Graysonb Department of Electrical Engineering and Computer Science, Northwestern University, Evanston

  10. Role of RNA Binding Proteins and Post-Transcriptional Regulation in Response to Environmental Changes in Arabidopsis thaliana

    E-Print Network [OSTI]

    Juntawong, Piyada

    2010-01-01

    Burd, C. G. 1993. hnRNP proteins and the biogenesis of mRNA.2002. Messenger-RNA-binding proteins and the messages theycruzi poly-zinc finger protein: a novel DNA/RNA-binding

  11. Characterization of anti-HIV-1 neutralizing and binding antibodies in chronic HIV-1 subtype C infection

    E-Print Network [OSTI]

    Archary, Derseree

    Neutralizing (nAbs) and high affinity binding antibodies may be critical for an efficacious HIV-1 vaccine. We characterized virus-specific nAbs and binding antibody responses over 21 months in eight HIV-1 subtype C chronically ...

  12. Sulfolobus shibatae CCA-adding Enzyme Forms a Tetramer upon Binding Two tRNA Molecules: A

    E-Print Network [OSTI]

    Li, Fang

    Sulfolobus shibatae CCA-adding Enzyme Forms a Tetramer upon Binding Two tRNA Molecules to form a tetramer is induced by the binding of two tRNA molecules. The formation of a tetramer with only

  13. Binding information in short-term memory: evidence from healthy individuals, Alzheimer's Disease and other clinical populations 

    E-Print Network [OSTI]

    Rodríguez, Mario Alfredo Parra

    Memory binding is a cognitive process that enables complex objects to be stored or retrieved coherently during perception, learning, or action. Binding functions are aimed at reducing the misattribution of the features ...

  14. Pipe line companies to install 14,766 miles of lines in 1994

    SciTech Connect (OSTI)

    Ives, G. Jr.

    1994-01-01

    This paper reviews the historical and projected pipeline construction activities in the US and around the world. It gives mileage values for all types of oil and gas transmission lines, both on and offshore. Tables provides information on the diameters of these pipelines and information on compressor stations planned and constructed. Known major construction projects are listed by company name with a brief description of the proposed project.

  15. The transcriptional diversity of 25 Drosophila cell lines

    SciTech Connect (OSTI)

    Cherbas, Lucy; Willingham, Aarron; Zhang, Dayu; Yang, Li; Zou, Yi; Eads, Brian D.; Carlson, Joseph W.; Landolin, Jane M.; Kapranov, Philipp; Dumais, Jacqueline; Samsonova, Anastasia; Choi, Jeong-Hyeon; Roberts, Johnny; Davis, Carrie A.; Tang, Haixu; van Baren, Marijke J.; Ghosh, Srinka; Dobin, Alexander; Bell, Kim; Lin, Wei; Langton, Laura; Duff, Michael O.; Tenney, Aaron E.; Zaleski, Chris; Brent, Michael R.; Hoskins, Roger A.; Kaufman, Thomas C.; Andrews, Justen; Graveley, Brenton R.; Perrimon, Norbert; Celniker, Susan E.; Gingeras, Thomas R.; Cherbas, Peter

    2010-11-15

    Drosophila melanogaster cell lines are important resources for cell biologists. Here, we catalog the expression of exons, genes, and unannotated transcriptional signals for 25 lines. Unannotated transcription is substantial (typically 19% of euchromatic signal). Conservatively, we identify 1405 novel transcribed regions; 684 of these appear to be new exons of neighboring, often distant, genes. Sixty-four percent of genes are expressed detectably in at least one line, but only 21% are detected in all lines. Each cell line expresses, on average, 5885 genes, including a common set of 3109. Expression levels vary over several orders of magnitude. Major signaling pathways are well represented: most differentiation pathways are ‘‘off’’ and survival/growth pathways ‘‘on.’’ Roughly 50% of the genes expressed by each line are not part of the common set, and these show considerable individuality. Thirty-one percent are expressed at a higher level in at least one cell line than in any single developmental stage, suggesting that each line is enriched for genes characteristic of small sets of cells. Most remarkable is that imaginal discderived lines can generally be assigned, on the basis of expression, to small territories within developing discs. These mappings reveal unexpected stability of even fine-grained spatial determination. No two cell lines show identical transcription factor expression. We conclude that each line has retained features of an individual founder cell superimposed on a common ‘‘cell line‘‘ gene expression pattern. Wereport the transcriptional profiles of 25 Drosophila melanogaster cell lines, principally by whole-genome tiling microarray analysis of total RNA, carried out as part of the modENCODE project. The data produced in this study add to our knowledge of the cell lines and of the Drosophila transcriptome in several ways. We summarize the expression of previously annotated genes in each of the 25 lines with emphasis on what those patterns reveal about the origins of the lines and the stability of spatial expression patterns. We also offer an initial analysis of previously unannotated transcripts in the cell lines.

  16. Sensor, method and system of monitoring transmission lines

    DOE Patents [OSTI]

    Syracuse, Steven J.; Clark, Roy; Halverson, Peter G.; Tesche, Frederick M.; Barlow, Charles V.

    2012-10-02

    An apparatus, method, and system for measuring the magnetic field produced by phase conductors in multi-phase power lines. The magnetic field measurements are used to determine the current load on the conductors. The magnetic fields are sensed by coils placed sufficiently proximate the lines to measure the voltage induced in the coils by the field without touching the lines. The x and y components of the magnetic fields are used to calculate the conductor sag, and then the sag data, along with the field strength data, can be used to calculate the current load on the line and the phase of the current. The sag calculations of this invention are independent of line voltage and line current measurements. The system applies a computerized fitter routine to measured and sampled voltages on the coils to accurately determine the values of parameters associated with the overhead phase conductors.

  17. Rotational Line Emission from Water in Protoplanetary Disks

    E-Print Network [OSTI]

    R. Meijerink; D. R. Poelman; M. Spaans; A. G. G. M. Tielens; A. E. Glassgold

    2008-10-09

    Circumstellar disks provide the material reservoir for the growth of young stars and for planet formation. We combine a high-level radiative transfer program with a thermal-chemical model of a typical T Tauri star disk to investigate the diagnostic potential of the far-infrared lines of water for probing disk structure. We discuss the observability of pure rotational H2O lines with the Herschel Space Observatory, specifically the residual gas where water is mainly frozen out. We find that measuring both the line profile of the ground 110-101 ortho-H2O transition and the ratio of this line to the 312-303 and 221-212 line can provide information on the gas phase water between 5-100 AU, but not on the snow line which is expected to occur at smaller radii.

  18. Radio frequency communication system utilizing radiating transmission lines

    DOE Patents [OSTI]

    Struven, Warren C. (San Carlos, CA)

    1984-01-01

    A radio communication system for use in tunnels, mines, buildings or other shielded locations in which a pair of radiating transmission lines (30), (31) extend through such location in spaced coextensive relation to each other. Each transmission line (30), (31) has at least one unidirectional amplifier (32), (33) interposed therein with the sense of the unidirectional amplifier (32) of one transmission line (30) being opposite to the sense of the unidirectional amplifier (33) of the other transmission line (31). Each of the amplifiers (32), (33) has a gain which is less than the coupling loss between the transmission lines (30), (31). Two or more mobile transceivers (35) in the location served by the system are coupled to the transmission lines (30), (31) by electromagnetic wave propagation in space in order to communicate directly with each other at a given radio frequency within the frequency range of the system.

  19. Slow sound in lined flow ducts

    E-Print Network [OSTI]

    Auregan, Yves

    2015-01-01

    We consider the acoustic propagation in lined flow duct with a purely reactive impedance at the wall. This reacting liner has the capability to reduce the speed of sound, and thus to enhance the interaction between the acoustic propagation and the low Mach number flow ($M\\simeq0.3$). At the lower frequencies, there are typically 4 acoustic or hydrodynamic propagating modes, with 3 of them propagating in the direction of the flow. Above a critical frequency, there are only 2 propagating modes that all propagate in the direction of the flow. From the exact 2D formulation an approximate 1D model is developed to study the scattering of acoustic waves in a straight duct with varying wall impedance. This simple system, with a uniform flow and with a non-uniform liner impedance at the wall, permits to study the scattering between regions with different waves characteristics. Several situations are characterized to show the importance of negative energy waves, strong interactions between acoustic and hydrodynamic mod...

  20. Second Line of Defense Spares Program

    SciTech Connect (OSTI)

    Henderson, Dale L.; Holmes, Aimee E.; Muller, George; Mercier, Theresa M.; Brigantic, Robert T.; Perkins, Casey J.; Cooley, Scott K.; Thorsen, Darlene E.

    2012-11-20

    During Fiscal Year 2012, a team from the Pacific Northwest National Laboratory (PNNL) conducted an assessment and analysis of the Second Line of Defense (SLD) Sustainability spare parts program. Spare parts management touches many aspects of the SLD Sustainability Program including contracting and integration of Local Maintenance Providers (LMP), equipment vendors, analyses and metrics on program performance, system state of health, and maintenance practices. Standardized spares management will provide better data for decisions during site transition phase and will facilitate transition to host country sustainability ownership. The effort was coordinated with related SLD Sustainability Program initiatives, including a configuration items baselining initiative, a metrics initiative, and a maintenance initiative. The spares study has also led to pilot programs for sourcing alternatives that include regional intermediate inventories and partnering agreements that leverage existing supply chains. Many partners from the SLD Sustainability program contributed to and were consulted in the course of the study. This document provides a description of the findings, recommendations, and implemented solutions that have resulted from the study.

  1. Boron-Lined Neutron Detector Measurements

    SciTech Connect (OSTI)

    Lintereur, Azaree T.; Kouzes, Richard T.; Ely, James H.; Erikson, Luke E.; Siciliano, Edward R.; Woodring, Mitchell L.

    2010-03-07

    PNNL-18938 Revision Radiation portal monitors used for interdiction of illicit materials at borders include highly sensitive neutron detection systems. The main reason for having neutron detection capability is to detect fission neutrons from plutonium. The currently deployed radiation portal monitors (RPMs) from Ludlum and Science Applications International Corporation (SAIC) use neutron detectors based upon 3He-filled gas proportional counters, which are the most common large neutron detector. There is a declining supply of 3He in the world, and thus, methods to reduce the use of this gas in RPMs with minimal changes to the current system designs and sensitivity to cargo-borne neutrons are being investigated. Four technologies have been identified as being currently commercially available, potential alternative neutron detectors to replace the use of 3He in RPMs. Reported here are the results of tests of a newly designed boron-lined proportional counter option. This testing measured the neutron detection efficiency and gamma ray rejection capabilities of two successive prototypes of a system manufactured by GE Reuter Stokes.

  2. Boron-Lined Neutron Detector Measurements

    SciTech Connect (OSTI)

    Lintereur, Azaree T.; Kouzes, Richard T.; Ely, James H.; Erikson, Luke E.; Siciliano, Edward R.

    2009-11-02

    Radiation portal monitors used for interdiction of illicit materials at borders include highly sensitive neutron detection systems. The main reason for having neutron detection capability is to detect fission neutrons from plutonium. The currently deployed radiation portal monitors (RPMs) from Ludlum and Science Applications International Corporation (SAIC) use neutron detectors based upon 3He-filled gas proportional counters, which are the most common large neutron detector. There is a declining supply of 3He in the world, and thus, methods to reduce the use of this gas in RPMs with minimal changes to the current system designs and sensitivity to cargo-borne neutrons are being investigated. Four technologies have been identified as being currently commercially available, potential alternative neutron detectors to replace the use of 3He in RPMs. Reported here are the results of tests of a newly designed boron-lined proportional counter option. This testing measured the neutron detection efficiency and gamma ray rejection capabilities of a system manufactured by Reuter Stokes.

  3. Contingency Analysis of Cascading Line Outage Events

    SciTech Connect (OSTI)

    Thomas L Baldwin; Magdy S Tawfik; Miles McQueen

    2011-03-01

    As the US power systems continue to increase in size and complexity, including the growth of smart grids, larger blackouts due to cascading outages become more likely. Grid congestion is often associated with a cascading collapse leading to a major blackout. Such a collapse is characterized by a self-sustaining sequence of line outages followed by a topology breakup of the network. This paper addresses the implementation and testing of a process for N-k contingency analysis and sequential cascading outage simulation in order to identify potential cascading modes. A modeling approach described in this paper offers a unique capability to identify initiating events that may lead to cascading outages. It predicts the development of cascading events by identifying and visualizing potential cascading tiers. The proposed approach was implemented using a 328-bus simplified SERC power system network. The results of the study indicate that initiating events and possible cascading chains may be identified, ranked and visualized. This approach may be used to improve the reliability of a transmission grid and reduce its vulnerability to cascading outages.

  4. Contact lines for fluid surface adhesion

    E-Print Network [OSTI]

    Markus Deserno; Martin M. Mueller; Jemal Guven

    2007-03-01

    When a fluid surface adheres to a substrate, the location of the contact line adjusts in order to minimize the overall energy. This adhesion balance implies boundary conditions which depend on the characteristic surface deformation energies. We develop a general geometrical framework within which these conditions can be systematically derived. We treat both adhesion to a rigid substrate as well as adhesion between two fluid surfaces, and illustrate our general results for several important Hamiltonians involving both curvature and curvature gradients. Some of these have previously been studied using very different techniques, others are to our knowledge new. What becomes clear in our approach is that, except for capillary phenomena, these boundary conditions are not the manifestation of a local force balance, even if the concept of surface stress is properly generalized. Hamiltonians containing higher order surface derivatives are not just sensitive to boundary translations but also notice changes in slope or even curvature. Both the necessity and the functional form of the corresponding additional contributions follow readily from our treatment.

  5. NMR studies of DNA oligomers and their interactions with minor groove binding ligands

    SciTech Connect (OSTI)

    Fagan, P A [Univ. of California, Berkeley, CA (United States). Dept. of Chemistry

    1996-05-01

    The cationic peptide ligands distamycin and netropsin bind noncovalently to the minor groove of DNA. The binding site, orientation, stoichiometry, and qualitative affinity of distamycin binding to several short DNA oligomers were investigated by NMR spectroscopy. The oligomers studied contain A,T-rich or I,C-rich binding sites, where I = 2-desaminodeoxyguanosine. I{center_dot}C base pairs are functional analogs of A{center_dot}T base pairs in the minor groove. The different behaviors exhibited by distamycin and netropsin binding to various DNA sequences suggested that these ligands are sensitive probes of DNA structure. For sites of five or more base pairs, distamycin can form 1:1 or 2:1 ligand:DNA complexes. Cooperativity in distamycin binding is low in sites such as AAAAA which has narrow minor grooves, and is higher in sites with wider minor grooves such as ATATAT. The distamycin binding and base pair opening lifetimes of I,C-containing DNA oligomers suggest that the I,C minor groove is structurally different from the A,T minor groove. Molecules which direct chemistry to a specific DNA sequence could be used as antiviral compounds, diagnostic probes, or molecular biology tools. The author studied two ligands in which reactive groups were tethered to a distamycin to increase the sequence specificity of the reactive agent.

  6. Assessing Energetic Contributions to Binding from a Disordered Region in a Protein-Protein Interaction

    SciTech Connect (OSTI)

    S Cho; C Swaminathan; D Bonsor; M Kerzic; R Guan; J Yang; C Kieke; P Anderson; D Kranz; et al.

    2011-12-31

    Many functional proteins are at least partially disordered prior to binding. Although the structural transitions upon binding of disordered protein regions can influence the affinity and specificity of protein complexes, their precise energetic contributions to binding are unknown. Here, we use a model protein-protein interaction system in which a locally disordered region has been modified by directed evolution to quantitatively assess the thermodynamic and structural contributions to binding of disorder-to-order transitions. Through X-ray structure determination of the protein binding partners before and after complex formation and isothermal titration calorimetry of the interactions, we observe a correlation between protein ordering and binding affinity for complexes along this affinity maturation pathway. Additionally, we show that discrepancies between observed and calculated heat capacities based on buried surface area changes in the protein complexes can be explained largely by heat capacity changes that would result solely from folding the locally disordered region. Previously developed algorithms for predicting binding energies of protein-protein interactions, however, are unable to correctly model the energetic contributions of the structural transitions in our model system. While this highlights the shortcomings of current computational methods in modeling conformational flexibility, it suggests that the experimental methods used here could provide training sets of molecular interactions for improving these algorithms and further rationalizing molecular recognition in protein-protein interactions.

  7. Monolithic torpedo bottle lining at Weirton Steel Corporation

    SciTech Connect (OSTI)

    Baker, R.; Griffith, E. [Weirton Steel Corp., WV (United States)

    1996-12-31

    In late 1992 and early 1993 Weirton Steel burned through three torpedo bottles in a three-month period. To determine the cause of the burn throughs, a thorough review of bottle maintenance practices was initiated. Upon identification of contributing factors, changes in operating practices were made. In an effort to increase bottle reliability, lining trials were initiated. Among the trials, a monolithic lining was installed and this paper will discuss results of the lining to date.

  8. TRANSPORT OF WASTE SIMULANTS IN PJM VENT LINES

    SciTech Connect (OSTI)

    Qureshi, Z

    2007-02-21

    The experimental work was conducted to determine whether there is a potential for waste simulant to transport or 'creep' up the air link line and contaminate the pulse jet vent system, and possibly cause long term restriction of the air link line. Additionally, if simulant creep occurred, establish operating parameters for washing down the line. The amount of the addition of flush fluids and mixer downtime must be quantified.

  9. Inertial confinement fusion method producing line source radiation fluence

    DOE Patents [OSTI]

    Rose, Ronald P. (Peters Township, Washington County, PA)

    1984-01-01

    An inertial confinement fusion method in which target pellets are imploded in sequence by laser light beams or other energy beams at an implosion site which is variable between pellet implosions along a line. The effect of the variability in position of the implosion site along a line is to distribute the radiation fluence in surrounding reactor components as a line source of radiation would do, thereby permitting the utilization of cylindrical geometry in the design of the reactor and internal components.

  10. A simple line wave generator using commercial explosives

    SciTech Connect (OSTI)

    Morris, John S; Jackson, Scott I; Hill, Larry G

    2009-01-01

    We present a simple and inexpensive explosive line wave generator has been designed using commercial sheet explosive and plane wave lens concepts. The line wave generator is constructed using PETN and RDX based sheet explosive for the slow and fast components respectively. The design permits the creation of any desired line width. A series of experiments were performed on a 100 mm design, measuring the detonation arrival time at the output of the generator using a streak camera. An iterative technique was used to adjust the line wave generator's slow and fast components, so as to minimize the arrival time deviation. Designs, test results, and concepts for improvements will be discussed.

  11. Electrical and Biological Effects of Transmission Lines: A Review.

    SciTech Connect (OSTI)

    Lee, Jack M.

    1989-06-01

    This review describes the electrical properties of a-c and d-c transmission lines and the resulting effects on plants, animals, and people. Methods used by BPA to mitigate undesirable effects are also discussed. Although much of the information in this review pertains to high-voltage transmission lines, information on distribution lines and electrical appliances is included. The electrical properties discussed are electric and magnetic fields and corona: first for alternating-current (a-c) lines, then for direct current (d-c).

  12. Emission Line Properties of the Large Bright Quasar Survey

    E-Print Network [OSTI]

    Karl Forster; Paul J. Green; Thomas L. Aldcroft; Marianne Vestergaard; Craig B. Foltz; Paul C. Hewett

    2000-11-20

    We present measurements of the optical/UV emission lines for a large homogeneous sample of 993 quasars from the Large Bright Quasar Survey. Our largely automated technique accounts for continuum breaks and galactic reddening, and we perform multicomponent fits to emission line profiles, including the effects of blended iron emission, and of absorption lines both galactic and intrinsic. Here we describe the fitting algorithm and present the results of line fits to the LBQS sample, including upper limits to line equivalent widths when warranted. The distribution of measured line parameters, principally equivalent width and FWHM, are detailed for a variety of lines, including upper limits. We thus initiate a large-scale investigation of correlations between the high energy continuum and emission lines in quasars, to be extended to complementary samples using similar techniques. High quality, reproducible measurements of emission lines for uniformly selected samples will advance our understanding of active galaxies, especially in a new era of large surveys selected by a variety of complementary methods.

  13. EIS-0474: Southline Transmission Line Project; Arizona and New...

    Broader source: Energy.gov (indexed) [DOE]

    consist of a new 225-mile transmission line between existing substations at Afton, New Mexico, and Apache, Arizona, and improvements to approximately 130 miles of existing...

  14. Proposed Southline Transmission Line Project - Volume 4 of 4...

    Office of Environmental Management (EM)

    2 Appendix J J-1 Southline Transmission Line Project Draft Environmental Impact Statement and Draft Resource Management Plan Amendment BLM LAND USE AUTHORIZATIONS 1...

  15. Secretary Chu Visits Russian Seaport, Checks Out Second Line...

    Energy Savers [EERE]

    for me? The Second Line of Defense program cracks down on nuclear smuggling by installing radiation detection equipment at ports, border crossings and airports around the world....

  16. EIS-0502: Hot Springs to Anaconda Transmission Line Rebuild Project...

    Energy Savers [EERE]

    Springs to Anaconda Transmission Line Rebuild Project, Montana SUMMARY DOE's Bonneville Power Administration (BPA) is preparing an EIS that will analyze the potential environmental...

  17. To: Mansueti, Lawrence Subject: RE: Pepco Scheduled Line Repair...

    Office of Environmental Management (EM)

    my concern that the above scheduled line outage was likely to cause Mirant plant emission exceedances, putting at risk the health of over 4000 Alexandrians residing near the...

  18. Historical Time Line and Information About the Hanford Site

    SciTech Connect (OSTI)

    Briggs Jr, David

    2001-03-07

    Historical time line of the Hanford Site spanning from 1940 through 1997, including photographs and other information regarding the town sites and living conditions.

  19. A 1.3 cm Line Survey toward Orion KL

    E-Print Network [OSTI]

    Gong, Y; Thorwirth, S; Spezzano, S; Menten, K M; Walmsley, C M; Wyrowski, F; Mao, R Q; Klein, B

    2015-01-01

    Orion KL has served as a benchmark for spectral line searches throughout the (sub)millimeter regime. The main goal is to systematically study spectral characteristics of Orion KL in the 1.3 cm band. We carried out a spectral line survey (17.9 GHz to 26.2 GHz) with the Effelsberg-100 m telescope towards Orion KL. We find 261 spectral lines, yielding an average line density of about 32 spectral features per GHz above 3$\\sigma$. The identified lines include 164 radio recombination lines (RRLs) and 97 molecular lines. A total of 23 molecular transitions from species known to exist in Orion KL are detected for the first time in the interstellar medium. Non-metastable 15NH3 transitions are detected in Orion KL for the first time. Based on the velocity information of detected lines and the ALMA images, the spatial origins of molecular emission are constrained and discussed. A narrow feature is found in SO2 ($8_{1,7}-7_{2,6}$), possibly suggesting the presence of a maser line. Column densities and fractional abundanc...

  20. Development of aerogel-lined targets for inertial confinement...

    Office of Scientific and Technical Information (OSTI)

    aerogel-lined targets for inertial confinement fusion experiments Braun, T 36 MATERIALS SCIENCE Abstract not provided Lawrence Livermore National Laboratory (LLNL), Livermore, CA...

  1. Notice of Availability of Plains & Eastern Clean Line Transmission...

    Broader source: Energy.gov (indexed) [DOE]

    converter stations in Oklahoma and Tennessee; an approximately 720-mile high voltage direct current (HVDC) transmission line; an alternating current (AC) collection system;...

  2. Magnetic Field Line Tracing Calculations for Conceptual PFC Design...

    Office of Scientific and Technical Information (OSTI)

    Conference: Magnetic Field Line Tracing Calculations for Conceptual PFC Design in the National Compact Stellarator Experiment Citation Details In-Document Search Title: Magnetic...

  3. ,"Energy","Water","Renewable","Petroleum","Alt. Fuel",,"On-Line...

    Broader source: Energy.gov (indexed) [DOE]

    Energy","Water","Renewable","Petroleum","Alt. Fuel",,"On-Line Data Collection System",,"Report Period","Due In",,,"Primary","Secondary","Secondary" 2003,,,"EMS4","Environmental...

  4. Section 1222 Program - Proposed Plains & Eastern Clean Line Project...

    Energy Savers [EERE]

    Electricity Policy Coordination and Implementation Transmission Planning Section 1222 of the Energy Policy Act 2005 Proposed Project: Plains & Eastern Clean Line ...

  5. EA-1880: Big Bend to Witten Transmission Line Project, South...

    Office of Environmental Management (EM)

    transmission line, all in South Dakota. Proposed action is related to the Keystone XL project (see DOEEIS-0433-S1). Public Comment Opportunities None available at this...

  6. Cell, Vol. 94, 181191, July 24, 1998, Copyright 1998 by Cell Press Crystal Structure of the Signal Sequence Binding

    E-Print Network [OSTI]

    Walter, Peter

    Cell, Vol. 94, 181­191, July 24, 1998, Copyright ©1998 by Cell Press Crystal Structure). During targeting, the Ffh- The crystal structure of the signal sequence binding 4.5S RNA complex binds domain is a four-helix to the structural plasticity necessary for SRP to bind bundle that is closely

  7. Free Energy Component Analysis for Drug Design: A Case Study of HIV-1 Protease-Inhibitor Binding

    E-Print Network [OSTI]

    Jayaram, Bhyravabotla

    Free Energy Component Analysis for Drug Design: A Case Study of HIV-1 Protease-Inhibitor Binding of the free energies of binding of protein-ligand complexes is presented. The method formulated involves, and the trajectories were analyzed via a thermodynamic cycle to calculate the binding free energies. The computed free

  8. 1 Plasmodium falciparum SSB Tetramer Binds 2 Single-Stranded DNA Only in a Fully Wrapped Mode

    E-Print Network [OSTI]

    Lohman, Timothy M.

    1 Plasmodium falciparum SSB Tetramer Binds 2 Single-Stranded DNA Only in a Fully Wrapped Mode 3 with numerous DNA repair and replication proteins. Ec- 24 SSB tetramers can bind ssDNA in multiple DNA binding in fully wrapped complexes with site sizes of 30 52­65 nt/tetramer. Pf-SSB does not transition to the more

  9. A Ternary Metal Binding Site in the C2 Domain of Phosphoinositide-Specific Phospholipase C-1,

    E-Print Network [OSTI]

    Williams, Roger L.

    A Ternary Metal Binding Site in the C2 Domain of Phosphoinositide-Specific Phospholipase C-1, Lars. Binding of these metal ions is observed in the active site of the catalytic TIM barrel and in the calcium adjacent binding sites in the C2 domain were observed for calcium and the other metal/enzyme complexes

  10. Method for detecting binding events using micro-X-ray fluorescence spectrometry

    DOE Patents [OSTI]

    Warner, Benjamin P. (Los Alamos, NM); Havrilla, George J. (Los Alamos, NM); Mann, Grace (Hong Kong, HK)

    2010-12-28

    Method for detecting binding events using micro-X-ray fluorescence spectrometry. Receptors are exposed to at least one potential binder and arrayed on a substrate support. Each member of the array is exposed to X-ray radiation. The magnitude of a detectable X-ray fluorescence signal for at least one element can be used to determine whether a binding event between a binder and a receptor has occurred, and can provide information related to the extent of binding between the binder and receptor.

  11. Studies on immunoglobulins and complement binding to the surface of Schistosoma mansoni 

    E-Print Network [OSTI]

    Rasmussen, Kathleen Ruth

    1983-01-01

    of these adsorbed immunoglobulins is presumed to be by the Fc portion of the immunoglobul1n to Fc receptors on the worm because such adsorbed immunoglobulins are able to bind the antigens to which they were elicited. Torpier et al. (26) demonstrated Fc receptors.... , Texas A&M University Chairman of Advisory Committee: Walter Michael Kemp (Ph. D. ) An indirect assay was used to detect binding of Fc associated specific anti-schistosome antibody (homospec1fic antibody) to adult male schistosomes. Binding...

  12. The impact of gas bulk rotation on the Ly? line

    SciTech Connect (OSTI)

    Garavito-Camargo, Juan N.; Forero-Romero, Jaime E.; Dijkstra, Mark E-mail: je.forero@uniandes.edu.co

    2014-11-10

    We present results of radiative transfer calculations to measure the impact of gas bulk rotation on the morphology of the Ly? emission line in distant galaxies. We model a galaxy as a sphere with an homogeneous mixture of dust and hydrogen at a constant temperature. These spheres undergo solid-body rotation with maximum velocities in the range 0-300 km s{sup –1} and neutral hydrogen optical depths in the range ?{sub H} = 10{sup 5}-10{sup 7}. We consider two types of source distributions in the sphere: central and homogeneous. Our main result is that rotation introduces a dependence of the line morphology with viewing angle and rotational velocity. Observations with a line of sight parallel to the rotation axis yield line morphologies similar to the static case. For lines of sight perpendicular to the rotation axis, both the intensity at the line center and the line width increase with rotational velocity. Along the same line of sight, the line becomes single peaked at rotational velocities close to half the line width in the static case. Notably, we find that rotation does not induce any spatial anisotropy in the integrated line flux, the escape fraction or the average number of scatterings. This is because Lyman scattering through a rotating solid-body proceeds identically to the static case. The only difference is the Doppler shift from the different regions in the sphere that move with respect to the observer. This allows us to derive an analytic approximation for the viewing-angle dependence of the emerging spectrum, as a function of rotational velocity.

  13. Demonstration of Datacenter Automation Software and Hardware (DASH) at the California Franchise Tax Board

    E-Print Network [OSTI]

    Bell, Geoffrey C.

    2010-01-01

    2009. Demonstration of Datacenter Automation Software andDemonstration of Datacenter Automation Software andand tested while other datacenter cooling best practices

  14. Cavity design and characteristics of monolithic long-wavelength InAs/InP quantum dash

    E-Print Network [OSTI]

    New Mexico, University of

    @unm.edu Abstract: By extending the net-gain modulation phasor approach to account for the discrete distribution. J. Malloy, and L. F. Lester, "Extremely Low Room-Temperature Threshold Current Density Diode Lasers­9 (1991). 13. Y.-C. Xin, Y. Li, A. Martinez, T. J. Rotter, H. Su, L. Zhang, A. L. Gray, S. Luong, K. Sun

  15. Opening night of L.A. Rebellion: Feature films by Julie Dash

    E-Print Network [OSTI]

    M, Witte

    2012-01-01

    Archive to collect, Black Cinema" featured the screening ofRebellion initiative Black cinema movement i n Los Angeles.an alternative Black cinema practice in ject to irreparable

  16. Two-photon Absorption In Quantum Dots,quantum Dashes And Related Materials

    SciTech Connect (OSTI)

    Jain, Ravinder

    2009-08-31

    We have proposed the use of USQDs for various deep-tissue biological imaging applications, notably wavelength-multiplexed multicolor imaging and intra-nuclear studies such as those involving cell apoptosis, and have studied the issue of maximizing two-photon absorption-induced fluorescence (TPAF) signals from CdSe/ZnS USQDs to be used for this application. In particular, using 2 nm USQDs, we have shown that the TPAF signal at 780 nm is ~ 8 times that at 850 nm and 68 times that at 900 nm, two wavelengths that have been used in previous studies using CdSe/ZnS SQDs for deep-tissue imaging of biological studies via TPAF .

  17. Demonstration of Datacenter Automation Software and Hardware (DASH) at the California Franchise Tax Board

    SciTech Connect (OSTI)

    Bell, Geoffrey C.; Federspiel, Clifford

    2009-12-18

    Control software and wireless sensors designed for closed-loop, monitoring and control of IT equipment's inlet air temperatures in datacenters were evaluated and tested while other datacenter cooling best practices were implemented. The controls software and hardware along with each best practice were installed sequentially and evaluated using a measurement and verification procedure between each measure. The results show that the overall project eliminates 475,239 kWh per year, which is 21.3percent of the baseline energy consumption of the data center. The total project, including the best practices will save $42,772 per year and cost $134,057 yielding a simple payback of 3.1 years. However, the control system alone eliminates 59.6percent of the baseline energy used to move air in the datacenter and 13.6percent of the baseline cooling energy, which is 15.2percent of the baseline energy consumption (see Project Approach, Task 1, below, for additional information) while keeping temperatures substantially within the limits recommended by ASHRAE. Savings attributed to the control system are $30,564 per year with a cost $56,824 for a simple payback of 1.9 years.

  18. Dash for Gas: The Sequel Christopher R. Knittel Konstantinos Metaxoglou Andre Trindade

    E-Print Network [OSTI]

    Rothman, Daniel

    costs by utilities that operate within wholesale power markets are more quickly absorbed into retail markets; utilities that operate in traditional vertically- integrated regions; independent power producers that do not operate in wholesale markets are more sensitive to changes in input prices than utilities

  19. Demonstration of Datacenter Automation Software and Hardware (DASH) at the California Franchise Tax Board

    E-Print Network [OSTI]

    Bell, Geoffrey C.

    2010-01-01

    distribution unit UPS: uninterruptible power supply VFD:at the center’s uninterruptible power supply (UPS). The

  20. Demonstration of Datacenter Automation Software and Hardware (DASH) at the California Franchise Tax Board

    E-Print Network [OSTI]

    Bell, Geoffrey C.

    2010-01-01

    air handlers with air-side economizers. References 1.handler, which has an air-side economizer. A study of how toair handlers with air-side economizers. Introduction Project

  1. Demonstration of Datacenter Automation Software and Hardware (DASH) at the California Franchise Tax Board

    E-Print Network [OSTI]

    Bell, Geoffrey C.

    2010-01-01

    unit UPS: uninterruptible power supply VFD: variableat the center’s uninterruptible power supply (UPS). The

  2. Flow Test At Fenton Hill HDR Geothermal Area (Dash, 1989) | Open Energy

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIX ECoopButtePowerEdistoWhiskey flatsInformation 7thFlorin,Information

  3. Flow Test At Fenton Hill HDR Geothermal Area (Dash, Et Al., 1983) | Open

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIX ECoopButtePowerEdistoWhiskey flatsInformation 7thFlorin,InformationEnergy

  4. Development Wells At Fenton Hill HDR Geothermal Area (Dash, Et Al., 1983) |

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIX ECoopButtePower VenturesInformation9)ask queries TypeDeveloper

  5. Observation Wells At Fenton Hill HDR Geothermal Area (Dash, Et Al., 1983) |

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIXsourceII Jump to:Information 3rd|Northfork ElectricName0

  6. Expression of low-, intermediate-, and high-affinity IL-2 receptors on B cell lines derived from patients with undifferentiated lymphoma of Burkitt's and non-Burkitt's types

    SciTech Connect (OSTI)

    Benjamin, D.; Rosolen, A.; Wormsley, S.B.; DeBault, L.E.; Colamonici, O.R. )

    1990-08-01

    IL-2 receptors on T cells exist in at least three forms which differ in their ligand-binding affinity. The low-affinity IL-2 receptor (IL-2R) consists of the 55-kDa Tac protein (p55 alpha), the intermediate-affinity site corresponds to the 70-kDa molecule (p70 beta), and the high-affinity IL-2R consists of a noncovalent heterodimeric structure involving both p55 alpha and p70 beta. We studied 24 B cell lines (8 EBV-negative and 16 EBV-positive) for IL-2R expression in the presence or absence of the tumor promoter, teleocidin. 125I-IL-2 radioreceptor binding assays and crosslinking studies demonstrated the sole expression of p55 alpha in EBV-negative cell lines only, whereas p55 alpha present in EBV-positive cell lines was always associated with p70 beta to construct high-affinity IL-2R. p70 beta was not detected in any of the EBV-negative cell lines, but was expressed on most of the EBV-positive cell lines (13 of 16). Our data also indicate that the expression of p55 alpha and p70 beta by radiolabeling correlates with their expression in flow cytometry, and that a large excess of p55 alpha is required to construct high-affinity IL-2R. Coexpression of p55 alpha and p70 beta on human B cells contributed to constructing high-affinity IL-2R hybrid complex as shown by rapid association rate contributed by p55 alpha and slow dissociation rate by p70 beta; teleocidin's ability to induce p55 alpha on cell lines which express p70 beta only, resulting in appearance of high-affinity IL-2R; and blocking p55 alpha by anti-Tac mAb in cell lines which constitutively express high-affinity IL-2R eliminated both high- and low-affinity components. The existence of low, intermediate, and high IL-2R on human B cells bears important future implications for understanding the mechanism of IL-2 signaling and the role of IL-2 in B cell activation, proliferation, and differentiation.

  7. Stoichiometry of Complexes between Mannose-binding Protein and Its Associated Serine Proteases

    E-Print Network [OSTI]

    of the innate immune response. It binds to sugars on the surfaces of pathogenic microorganisms and activates on the surfaces of microorganisms and activating two MBP-associated serine proteases (MASP-1 and MASP-2). Rat

  8. Engineering Encodable Lanthanide-Binding Tags (LBTs) into Loop Regions of Proteins

    E-Print Network [OSTI]

    Barthelmes, Katja

    Lanthanide-binding tags (LBTs) are valuable tools for investigation of protein structure, function, and dynamics by NMR spectroscopy, X-ray crystallography, and luminescence studies. We have inserted LBTs into three different ...

  9. Rational Design of New Binding Specificity by Simultaneous Mutagenesis of Calmodulin and a Target Peptide

    E-Print Network [OSTI]

    Green, David F.

    Calcium-saturated calmodulin (CaM) binds and influences the activity of a varied collection of target proteins in most cells. This promiscuity underlies the role of CaM as a shared participant in calcium-dependent signal ...

  10. Structural and functional consequences of platinum anticancer drug binding to free and nucleosomal DNA

    E-Print Network [OSTI]

    Todd, Ryan Christopher, 1981-

    2010-01-01

    Cisplatin, carboplatin, and oxaliplatin are three FDA-approved members of the platinum anticancer drug family. These compounds induce apoptosis in tumor cells by binding to nuclear DNA, forming a variety of adducts, and ...

  11. Control of HslUV protease function by nucleotide binding and hydrolysis

    E-Print Network [OSTI]

    Yakamavich, Joseph Andrew

    2008-01-01

    Many proteins act as molecular machines, using the power of nucleotide binding and hydrolysis to drive conformational changes in themselves and their target substrates. Like other AAA+ proteases, HslUV recognizes, unfolds, ...

  12. Analysis of variation at transcription factor binding sites in Drosophila and humans

    E-Print Network [OSTI]

    Spivakov, Mikhail

    Background: Advances in sequencing technology have boosted population genomics and made it possible to map the positions of transcription factor binding sites (TFBSs) with high precision. Here we investigate TFBS variability ...

  13. 2D IR spectroscopy and computational modeling : application to protein folding and binding

    E-Print Network [OSTI]

    Ganim, Ziad

    2010-01-01

    In this thesis, dynamics experiments are developed that can be used to study protein conformational changes such as folding and binding. Every functional motion of a protein is inextricably linked to conformational dynamics. ...

  14. Negatively Cooperative Binding of High-Density Lipoprotein to the HDL Receptor SR-BI

    E-Print Network [OSTI]

    Xu, Shangzhe

    Scavenger receptor class B, type I (SR-BI), is a high-density lipoprotein (HDL) receptor, which also binds low-density lipoprotein (LDL), and mediates the cellular selective uptake of cholesteryl esters from lipoproteins. ...

  15. Identification and analysis of hepatitis C virus NS3 helicase inhibitors using nucleic acid binding assays

    E-Print Network [OSTI]

    Mukherjee, Sourav; Hanson, Alica M.; Shadrick, William R.; Ndjomou, Jean; Sweeney, Noreena L.; Hernadez, John J.; Bartczak, Diana; Li, Kelin; Frankowski, Kevin J.; Heck, Julie A.; Arnold, Leggy A.; Schoenen, Frank; Frick, David N,

    2012-06-27

    Typical assays used to discover and analyze small molecules that inhibit the hepatitis C virus (HCV) NS3 helicase yield few hits and are often confounded by compound interference. Oligonucleotide binding assays are examined here as an alternative...

  16. V-058: Microsoft Internet Explorer CDwnBindInfo Object Reuse...

    Broader source: Energy.gov (indexed) [DOE]

    Microsoft Internet Explorer CDwnBindInfo Object Reuse Flaw Lets Remote Users Execute Arbitrary Code PLATFORM: Version(s): 6, 7, 8 ABSTRACT: A vulnerability was reported in...

  17. LETTER TO THE EDITOR Binding of manganese(II) to a tertiary stabilized

    E-Print Network [OSTI]

    Westhof, Eric

    as studied by electron paramagnetic resonance spectroscopy NATALIA KISSELEVA,1 ANASTASIA KHVOROVA,2 ERICII ions to a tertiary stabilized hammer- head ribozyme (tsHHRz) and to compare it with the binding

  18. Context influences on TALE–DNA binding revealed by quantitative profiling

    E-Print Network [OSTI]

    Rogers, Julia M.

    Transcription activator-like effector (TALE) proteins recognize DNA using a seemingly simple DNA-binding code, which makes them attractive for use in genome engineering technologies that require precise targeting. Although ...

  19. Water growth on metals and oxides: binding, dissociation and role of hydroxyl groups

    E-Print Network [OSTI]

    Salmeron, M.

    2008-01-01

    Note: In this paper the water structures formed are due toand correspond to a second water layer, not the first (seeWater growth on metals and oxides: binding, dissociation and

  20. How the Nature of Information Affects Binding in Visual Working Memory 

    E-Print Network [OSTI]

    Walt, Nicola

    2007-01-01

    The question of whether binding information affects the capacity of visual working memory has not been established to date. Different trends in thought have hypothesized different effects for the way information is stored ...

  1. Engineering and targeting glycan receptor binding of influenza A virus hemagglutinin

    E-Print Network [OSTI]

    Jayaraman, Akila

    2011-01-01

    The critical first step in the host infection by influenza A virus is the binding of the viral surface glycoprotein hemagglutinin (HA) to the sialylated glycan receptors terminated by N-acetyineuraminic acid (Neu5Ac) ...

  2. The Moving Contact Line in a Shallow Water Model

    E-Print Network [OSTI]

    Bridson, Robert

    The Moving Contact Line in a Shallow Water Model by Albert C. Wong B.Sc., The University of British with a shallow water model allowing us to track the contact line of the fluid in the shallow water model equation approach. ii #12;Table of Contents Abstract

  3. Synchronization phenomena for coupled delay-line oscillators

    E-Print Network [OSTI]

    Chicone, Carmen

    F@missouri.edu. 1 #12;1 Introduction Delay equation models for surface acoustic wave (SAW) delay-line devices-built surface acoustic wave (SAW) delay-line oscillators is examined. A bifurcation analysis reveals, averaging, Hopf bifurca- tion, Surface acoustic waves. AMS Classification: 34K60 Corresponding author

  4. Specification-based Testing for Software Product Lines Temesghen Kahsai

    E-Print Network [OSTI]

    Schlingloff, Holger

    Specification-based Testing for Software Product Lines Temesghen Kahsai Markus Roggenbach Bernd-Holger Schlingloff Abstract In this paper, we develop a testing theory for specification-based software product line development. Starting with a framework for the evaluation of test cases with respect to formal specifications

  5. Product-Line-Based Requirements Customization for Web Service Compositions

    E-Print Network [OSTI]

    Lutz, Robyn R.

    Product-Line-Based Requirements Customization for Web Service Compositions Hongyu Sun1 , Robyn Lutz composite web services by incorporating a software product-line engineering approach into web-service the resulting web- service compositions. By first creating a web-service composition search space that satisfies

  6. Transmission Line Boundary Protection Using Wavelet Transform and Neural Network

    E-Print Network [OSTI]

    is verified using frequency-dependent transmission line model and the test results prove its enhanced use data from two ends. The non-unit protection such as distance relay, can not protect the entire end only. In this case, the relay at one end can protect the entire line length with no intentional

  7. Transmission Line Resistance Compression Networks for Microwave Rectifiers

    E-Print Network [OSTI]

    Perreault, Dave

    Transmission Line Resistance Compression Networks for Microwave Rectifiers Taylor W. Barton, Joshua--This work presents a development of multi-way transmission-line resistance compression networks (TLRCNs) and their application to rf-to-dc conversion. We derive analytical expressions for the behavior of TLRCNs, and describe

  8. Pipelineable On-Line Encryption Farzaneh Abed1

    E-Print Network [OSTI]

    International Association for Cryptologic Research (IACR)

    Pipelineable On-Line Encryption Farzaneh Abed1 , Scott Fluhrer2 , Christian Forler1, Eik List1 chosen-ciphertext attacks with pipelineability to support efficient implementations. POE combines a block POET, a provably secure on-line AE scheme, which inherits pipelineability and chosen

  9. Situ soil sampling probe system with heated transfer line

    DOE Patents [OSTI]

    Robbat, Jr., Albert (Andover, MA)

    2002-01-01

    The present invention is directed both to an improved in situ penetrometer probe and to a heated, flexible transfer line. The line and probe may be implemented together in a penetrometer system in which the transfer line is used to connect the probe to a collector/analyzer at the surface. The probe comprises a heater that controls a temperature of a geologic medium surrounding the probe. At least one carrier gas port and vapor collection port are located on an external side wall of the probe. The carrier gas port provides a carrier gas into the geologic medium, and the collection port captures vapors from the geologic medium for analysis. In the transfer line, a flexible collection line that conveys a collected fluid, i.e., vapor, sample to a collector/analyzer. A flexible carrier gas line conveys a carrier gas to facilitate the collection of the sample. A system heating the collection line is also provided. Preferably the collection line is electrically conductive so that an electrical power source can generate a current through it so that the internal resistance generates heat.

  10. Transient Behavior of Two-Machine Geometric Production Lines

    E-Print Network [OSTI]

    Shimkin, Nahum

    Transient Behavior of Two-Machine Geometric Production Lines Semyon M. Meerkov Nahum Shimkin. Keywords: Production lines; Geometric reliability model; Production rate; Transient behavior; Effects of up to technological constraints; this leads to production losses in the subsequent shift (until the buffer occupancy

  11. Walleye fingerling culture in lined and earthen substrate ponds

    E-Print Network [OSTI]

    Walleye fingerling culture in lined and earthen substrate ponds Matthew J. Ward #12;Objectives 1://www.lakevermilionrealestate #12;Study Site, Design, and Statistics · Experimental unit was a 0.8-ha pond ­ Two treatments: Lined-test ­ Significance set at 0.05 * Blue Dog Lake State Fish Hatchery #12;Liner Material · Firestone Product ­ Pond

  12. On the observability of optically thin coronal hyperfine structure lines

    SciTech Connect (OSTI)

    Chatzikos, M.; Ferland, G. J. [University of Kentucky, Lexington, KY 40506 (United States); Williams, R. J. R. [AWE plc, Aldermaston, Reading RG7 4PR (United Kingdom); Fabian, A. C., E-mail: mchatzikos@gmail.com [Institute of Astronomy, Madingley Road, Cambridge CB3 0HA (United Kingdom)

    2014-06-01

    We present CLOUDY calculations for the intensity of coronal hyperfine lines in various environments. We model indirect collisional and radiative transitions, and quantify the collisionally excited line emissivity in the density-temperature phase space. As an observational aid, we also express the emissivity in units of that in the 0.4-0.7 keV band. For most hyperfine lines, knowledge of the X-ray surface brightness and the plasma temperature is sufficient for rough estimates. We find that the radiation fields of both Perseus A and Virgo A can enhance the populations of highly ionized species within 1 kpc. They can also enhance line emissivity within the cluster core. This could have implications for the interpretation of spectra around bright active galactic nuclei. We find the intensity of the {sup 57}Fe XXIV ?3.068 mm line to be about two orders of magnitude fainter than previously thought, at ?20 ?K. Comparably bright lines may be found in the infrared. Finally, we find the intensity of hyperfine lines in the Extended Orion Nebula to be low, due to the shallow sightline. Observations of coronal hyperfine lines will likely be feasible with the next generation of radio and submillimeter telescopes.

  13. Distributed MEMS Transmission Lines for Tunable Filter Applications

    E-Print Network [OSTI]

    York, Robert A.

    Distributed MEMS Transmission Lines for Tunable Filter Applications Yu Liu, Andrea Borgioli, Amit S: This paper describes the design and fabrication of a distributed MEMS ( )transmission line DMTL , used is a coplanar waveguide periodically loaded with continuously-variable MEMS capacitors. A tunable bandpass

  14. Transthoracic Lines Infants and children undergoing open heart

    E-Print Network [OSTI]

    Kay, Mark A.

    physician. All lines should have either heparinized saline or D5W infusing. Change the dressing with sternal by side. Normal RA values are 1-7mmHg. Transduce one port; vasoactive infusions typically are in the other port. Avoid infusing other solutions with vasoactive infusions. The RA line may be used for continuous

  15. Optimization of Automated Float Glass Lines Byungsoo Na, Shabbir Ahmed

    E-Print Network [OSTI]

    Ahmed, Shabbir

    Optimization of Automated Float Glass Lines Byungsoo Na, Shabbir Ahmed , George Nemhauser and Joel flat glass products being manufactured on float glass lines. New technologies are allowing float glass manufacturers to increase the level of automation in their plants, but the question of how to effectively use

  16. Development of nuclear receptor transfected Caco-2 cell lines

    E-Print Network [OSTI]

    Korjamo, Timo

    2006-10-27

    DEVELOPMENT OF NUCLEAR RECEPTOR TRANSFECTED CACO-2 CELL LINES Timo Korjamo University of Kuopio Finland 27th October 2006 ? Background ? Cell lines ? Gene expression ? Functional experiments ? Conclusions Intestinal absorption ? Small intestine... Reinisalo ? Technical assistance: Paula Nyyss?nen, Markku Taskinen ? Collaboration: Professor Pelkonen's group (Univ. Oulu, Finland) ? Funding: Finnish Funding Agency for Technology and Innovation (TEKES) ...

  17. Effects of dietary fiber and carcinogen on fatty acid binding protein expression in exfoliated colonocytes 

    E-Print Network [OSTI]

    Clark, Amy Eunice

    1997-01-01

    EFFECTS OF DIETARY FIBER AND CARCINOGEN ON FATTY ACID BINDING PROTEIN EXPRESSION IN EXFOLIATED COLONOCYTES A Thesis by AMY EUNICE CLARK Submitted to the Office of Gmduate Studies of Texas A&M University in partial fulfillment... of the requireinents for the degree of MASTER OF SCIENCE August 1997 Major Subject: Nutriuon EFFECTS OF DIETARY FIBER AND CARCINOGEN ON FATTY ACID BINDING PROTEIN EXPRESSION IN EXFOLlATED COLONOCYTES by AMY EUNICE CLARK Submitted to Texas ARM University...

  18. Statistical fluctuations of ground-state energies and binding energies in nuclei

    E-Print Network [OSTI]

    A. Molinari; H. A. Weidenmueller

    2004-03-11

    The statistical fluctuations of the ground-state energy and of the binding energy of nuclei are investigated using both perturbation theory and supersymmetry. The fluctuations are induced by the experimentally observed stochastic behavior of levels in the vicinity of neutron threshold. The results are compared with a recent analysis of binding-energy fluctuations by Bohigas and Leboeuf, and with theoretical work by Feshbach et al.

  19. Fe xxv line profiles in colliding wind binaries

    E-Print Network [OSTI]

    Rauw, Gregor; Naze, Yael

    2015-01-01

    Strong wind-wind collisions in massive binaries generate a very hot plasma that frequently produces a moderately strong iron line. The morphology of this line depends upon the properties of the wind interaction zone and its orientation with respect to the line of sight. As the binary components revolve around their common centre of mass, the line profiles are thus expected to vary. With the advent of the next generation of X-ray observatories (Astro-H, Athena) that will offer high-resolution spectroscopy above 6 keV, it will become possible to exploit these changes as the most sensitive probe of the inner parts of the colliding wind interaction. Using a simple prescription of the wind-wind interaction in an early-type binary, we have generated synthetic line profiles for a number of configurations and orbital phases. These profiles can help constrain the properties of the stellar winds in such binary systems.

  20. Continuous human cell lines and method of making same

    DOE Patents [OSTI]

    Stampfer, M.R.

    1985-07-01

    Substantially genetically stable continuous human cell lines derived from normal human mammary epithelial cells (HMEC) and processes for making and using the same. In a preferred embodiment, the cell lines are derived by treating normal human mammary epithelial tissue with a chemical carcinogen such as benzo(a)pyrene. The novel cell lines serve as useful substrates for elucidating the potential effects of a number of toxins, carcinogens and mutagens as well as of the addition of exogenous genetic material. The autogenic parent cells from which the cell lines are derived serve as convenient control samples for testing. The cell lines are not neoplastically transformed, although they have acquired several properties which distinguish them from their normal progenitors. 2 tabs.

  1. Theoretical X-ray Line Profiles from Colliding Wind Binaries

    E-Print Network [OSTI]

    Henley, D B; Pittard, J M

    2003-01-01

    We present theoretical X-ray line profiles from a range of model colliding wind systems. In particular, we investigate the effects of varying the stellar mass-loss rates, the wind speeds, and the viewing orientation. We find that a wide range of theoretical line profile shapes is possible, varying with orbital inclination and phase. At or near conjunction, the lines have approximately Gaussian profiles, with small widths (HWHM ~ 0.1 v_infty) and definite blue- or redshifts (depending on whether the star with the weaker wind is in front or behind). When the system is viewed at quadrature, the lines are generally much broader (HWHM ~ v_infty), flat-topped and unshifted. Local absorption can have a major effect on the observed profiles - in systems with mass-loss rates of a few times 10^{-6} Msol/yr the lower energy lines (E wind of the primary. The orbital variation ...

  2. Line graphs e contrazioni: un approccio rigoroso alla space syntax

    E-Print Network [OSTI]

    D'Autilia, Roberto

    2015-01-01

    The methods of the space syntax have been the subject of extensive discussion, and several techniques to identify the axis lines have been proposed. The space syntax can be represented in terms of line graph, a graphs defined on the edge of a given primary graph. By means of the line graph algorithms, a system of labels defined on the edges of the primary graph is transformed into a system of labels on the vertices of the line graph. The contraction of adjacent edges with the same label allows to build a more general graph than those generated with the methods of the space syntax. By means of the functions implemented in Mathematica is therefore possible to redefine the space syntax on any system of urban quantities (labels) and reproduce the results of the axial lines as a special case. As an example is discussed a possible application of the method to the urban noise analysis.

  3. Analysis of three and two coupled lines and design of a planar coupled-line bandpass filter and of baluns suitable for MIC and MMIC 

    E-Print Network [OSTI]

    Schwindt, Randall Scott

    1993-01-01

    Coupled line structures in inhomogeneous media are analyzed and applied in three circuits, a novel three-coupled line bandpass filter having sharp cutoff and two planar baluns composed of two sections of broadside-coupled lines. These are very...

  4. Profiled spectral lines generated in the field of Kerr superspinars

    SciTech Connect (OSTI)

    Schee, Jan; Stuchlík, Zdenek E-mail: zdenek.stuchlik@fpf.slu.cz

    2013-04-01

    String Theory suggests existence of primordial Kerr superspinars, extremely compact objects with external spacetime described by the Kerr naked singularity geometry. The primordial Kerr superspinars have to be converted to a near-extreme black hole due to accretion, but they could survive to the era of highly redshifted quasars. We study the shape of the profiled spectral lines generated by radiating rings or the innermost parts of Keplerian discs orbiting the Kerr superspinars. Influence of the superspinar surface location on the profiled lines is also considered. We demonstrate strong difference of the character of the profiled lines generated by radiating rings for all values of the superspinar spin and all values of the inclination angles of the observer when compared to those generated in the field of Kerr black holes. For small and mediate inclination angles there are large quantitative differences in the extension and position of the lines. For large inclination angles even strong qualitative difference appears as the profiled lines have a clear doubled character. The smaller, redshifted region of the profiled line is related to the photons reaching the regions near the superspinar surface. Strong differences are obtained also for profiled lines generated by the innermost parts of Keplerian discs especially in the shape of the line. The influence of the superspinar surface location is reflected in the intermediate parts of the the profiled lines. The line profiles can give a clear signature of the presence of a Kerr superspinar and in principle enable estimates of its surface location since the signatures of the superspinar surface location are of different character as those corresponding to the presence of the black hole horizon.

  5. Elevated epidermal growth factor receptor binding in plutonium-induced lung tumors from dogs

    SciTech Connect (OSTI)

    Leung, F.C.; Bohn, L.R.; Dagle, G.E. )

    1991-04-01

    The objective of this study is to examine and characterize epidermal growth factor receptor (EGF-R) binding in inhaled plutonium-induced canine lung-tumor tissue and to compare it with that in normal canine lung tissue. Crude membrane preparations from normal and lung-tumor tissue from beagle dogs were examined in a radioreceptor assay, using {sup 125}I-labeled epidermal growth factor (EGF) as a ligand. Specific EGF receptor binding was determined in the presence of excess unlabeled EGF. We have examined EGF receptor binding in eight lung-tumor samples obtained from six dogs. Epidermal growth factor receptor binding was significantly greater in lung-tumor samples (31.38%) compared with that in normal lung tissue (3.76%). Scatchard plot analysis from the displacement assay revealed that there was no statistical difference in the binding affinity but significantly higher concentration of EGF-R sites in the lung-tumor tissue (619 fmol/mg) than in normal lung tissue (53 fmol/mg). The increase in EGF-R number in plutonium-induced dog lung tumors does not seem to correlate with increase in the initial lung burden exposure to plutonium. Our results demonstrate that there is a significant increase in EGF-R binding in inhaled plutonium-induced dog lung tumors.

  6. In silico identification of anthropogenic chemicals as ligands of zebrafish sex hormone binding globulin

    SciTech Connect (OSTI)

    Thorsteinson, Nels; Ban, Fuqiang; Santos-Filho, Osvaldo; Tabaei, Seyed M.H. [Prostate Centre at the Vancouver General Hospital, University of British Columbia, 2660 Oak Street, Vancouver, BC, V6H 3Z6 (Canada); Miguel-Queralt, Solange; Underhill, Caroline [Department of Obstetrics and Gynecology, University of British Columbia, Child and Family Research Institute, 950 West 28th Avenue, Vancouver, BC, V5Z 4H4 (Canada); Cherkasov, Artem [Prostate Centre at the Vancouver General Hospital, University of British Columbia, 2660 Oak Street, Vancouver, BC, V6H 3Z6 (Canada)], E-mail: artc@interchange.ubc.ca; Hammond, Geoffrey L. [Department of Obstetrics and Gynecology, University of British Columbia, Child and Family Research Institute, 950 West 28th Avenue, Vancouver, BC, V5Z 4H4 (Canada)

    2009-01-01

    Anthropogenic compounds with the capacity to interact with the steroid-binding site of sex hormone binding globulin (SHBG) pose health risks to humans and other vertebrates including fish. Building on studies of human SHBG, we have applied in silico drug discovery methods to identify potential binders for SHBG in zebrafish (Danio rerio) as a model aquatic organism. Computational methods, including; homology modeling, molecular dynamics simulations, virtual screening, and 3D QSAR analysis, successfully identified 6 non-steroidal substances from the ZINC chemical database that bind to zebrafish SHBG (zfSHBG) with low-micromolar to nanomolar affinities, as determined by a competitive ligand-binding assay. We also screened 80,000 commercial substances listed by the European Chemicals Bureau and Environment Canada, and 6 non-steroidal hits from this in silico screen were tested experimentally for zfSHBG binding. All 6 of these compounds displaced the [{sup 3}H]5{alpha}-dihydrotestosterone used as labeled ligand in the zfSHBG screening assay when tested at a 33 {mu}M concentration, and 3 of them (hexestrol, 4-tert-octylcatechol, and dihydrobenzo(a)pyren-7(8H)-one) bind to zfSHBG in the micromolar range. The study demonstrates the feasibility of large-scale in silico screening of anthropogenic compounds that may disrupt or highjack functionally important protein:ligand interactions. Such studies could increase the awareness of hazards posed by existing commercial chemicals at relatively low cost.

  7. Alpha-2 Heremans Schmid Glycoprotein (AHSG) Modulates Signaling Pathways in Head and Neck Squamous Cell Carcinoma Cell Line SQ20B

    SciTech Connect (OSTI)

    Thompson, Pamela D.; Sakwe, Amos [Department of Biochemistry and Cancer Biology, Meharry Medical College, Nashville, TN 37208 (United States); Koumangoye, Rainelli [Division of Surgical Oncology and Endocrine Surgery, Vanderbilt University Medical Center, Nashville, TN 37232 (United States); Yarbrough, Wendell G. [Division of Otolaryngology, Departments of Surgery and Pathology and Yale Cancer Center, Yale University, New Haven, CT 06520 (United States); Ochieng, Josiah [Department of Biochemistry and Cancer Biology, Meharry Medical College, Nashville, TN 37208 (United States); Marshall, Dana R., E-mail: dmarshall@mmc.edu [Department of Pathology, Anatomy and Cell Biology, Meharry Medical College, Nashville, TN 37208 (United States)

    2014-02-15

    This study was performed to identify the potential role of Alpha-2 Heremans Schmid Glycoprotein (AHSG) in Head and Neck Squamous Cell Carcinoma (HNSCC) tumorigenesis using an HNSCC cell line model. HNSCC cell lines are unique among cancer cell lines, in that they produce endogenous AHSG and do not rely, solely, on AHSG derived from serum. To produce our model, we performed a stable transfection to down-regulate AHSG in the HNSCC cell line SQ20B, resulting in three SQ20B sublines, AH50 with 50% AHSG production, AH20 with 20% AHSG production and EV which is the empty vector control expressing wild-type levels of AHSG. Utilizing these sublines, we examined the effect of AHSG depletion on cellular adhesion, proliferation, migration and invasion in a serum-free environment. We demonstrated that sublines EV and AH50 adhered to plastic and laminin significantly faster than the AH20 cell line, supporting the previously reported role of exogenous AHSG in cell adhesion. As for proliferative potential, EV had the greatest amount of proliferation with AH50 proliferation significantly diminished. AH20 cells did not proliferate at all. Depletion of AHSG also diminished cellular migration and invasion. TGF-? was examined to determine whether levels of the TGF-? binding AHSG influenced the effect of TGF-? on cell signaling and proliferation. Whereas higher levels of AHSG blunted TGF-? influenced SMAD and ERK signaling, it did not clearly affect proliferation, suggesting that AHSG influences on adhesion, proliferation, invasion and migration are primarily due to its role in adhesion and cell spreading. The previously reported role of AHSG in potentiating metastasis via protecting MMP-9 from autolysis was also supported in this cell line based model system of endogenous AHSG production in HNSCC. Together, these data show that endogenously produced AHSG in an HNSCC cell line, promotes in vitro cellular properties identified as having a role in tumorigenesis. Highlights: • Head and neck squamous cell carcinoma cell lines synthesize and secret AHSG. • AHSG depleted cell lines are significantly inhibited in their ability to proliferate, adhere, migrate, invade and protect MMP-9. • Human AHSG and bovine fetuin-A are functionally equivalent in regards to growth promotion of cancer cell lines.

  8. On-line Module Selection Student Guidelines On-line module selection opens on Wed 18th

    E-Print Network [OSTI]

    Harman, Neal.A.

    On-line Module Selection Student Guidelines Step 1 On-line module selection opens on Wed 18th March' followed by the tab `Next Year's Module Pre-selections'. Please note that any modules that are compulsory programme, you will need to submit the paper-work for this in-advance of selecting modules as your online

  9. On-line versus o-line vestibular-evoked control of goal-directed arm movements

    E-Print Network [OSTI]

    On-line versus o¡-line vestibular-evoked control of goal-directed arm movements Jean-directed arm movements towards memorized visual targets when the whole body is passively ro- tated during movement execution. Subjects succeededin compen- sating for current body rotation by regulating ongoing arm

  10. Functional characterization of acyl-CoA binding protein (ACBP) and oxysterol binding protein-related proteins (ORPS) from Cryptosporidium parvum 

    E-Print Network [OSTI]

    Zeng, Bin

    2009-05-15

    From opportunistic protist Cryptosporidium parvum we identified and functionally assayed a fatty acyl-CoA-binding protein (ACBP) gene. The CpACBP1 gene encodes a protein of 268 aa that is three times larger than typical ~10 KD ACBPs of humans...

  11. On the Steady Nature of Line-Driven Disk Winds

    E-Print Network [OSTI]

    Nicolas A. Pereyra; Stanley P. Owocki; D. John Hillier; David A. Turnshek

    2003-11-11

    We perform an analytic investigation of the stability of line-driven disk winds, independent of hydrodynamic simulations. Our motive is to determine whether or not line-driven disk winds can account for the wide/broad UV resonance absorption lines seen in cataclysmic variables (CVs) and quasi-stellar objects (QSOs). In both CVs and QSOs observations generally indicate that the absorption arising in the outflowing winds has a steady velocity structure on time scales exceeding years (for CVs) and decades (for QSOs). However, published results from hydrodynamic simulations of line-driven disk winds are mixed, with some researchers claiming that the models are inherently unsteady, while other models produce steady winds. The analytic investigation presented here shows that if the accretion disk is steady, then the line-driven disk wind emanating from it can also be steady. In particular, we show that a gravitational force initially increasing along the wind streamline, which is characteristic of disk winds, does not imply an unsteady wind. The steady nature of line-driven disk winds is consistent with the 1D streamline disk-wind models of Murray and collaborators and the 2.5D time-dependent models of Pereyra and collaborators. This paper emphasizes the underlying physics behind the steady nature of line-driven disk winds using mathematically simple models that mimic the disk environment

  12. Theoretical X-ray Line Profiles from Colliding Wind Binaries

    E-Print Network [OSTI]

    D. B. Henley; I. R. Stevens; J. M. Pittard

    2003-06-23

    We present theoretical X-ray line profiles from a range of model colliding wind systems. In particular, we investigate the effects of varying the stellar mass-loss rates, the wind speeds, and the viewing orientation. We find that a wide range of theoretical line profile shapes is possible, varying with orbital inclination and phase. At or near conjunction, the lines have approximately Gaussian profiles, with small widths (HWHM ~ 0.1 v_\\infty) and definite blue- or redshifts (depending on whether the star with the weaker wind is in front or behind). When the system is viewed at quadrature, the lines are generally much broader (HWHM ~ v_\\infty), flat-topped and unshifted. Local absorption can have a major effect on the observed profiles - in systems with mass-loss rates of a few times 10^{-6} Msol/yr the lower energy lines (E wind of the primary. The orbital variation of the line widths and shifts is reduced in a low inclination binary. The extreme case is a binary with i = 0 degrees, for which we would expect no line profile variation.

  13. Stainless steel submerged arc weld fusion line toughness

    SciTech Connect (OSTI)

    Rosenfield, A.R.; Held, P.R.; Wilkowski, G.M. [Battelle, Columbus, OH (United States)

    1995-04-01

    This effort evaluated the fracture toughness of austenitic steel submerged-arc weld (SAW) fusion lines. The incentive was to explain why cracks grow into the fusion line in many pipe tests conducted with cracks initially centered in SAWS. The concern was that the fusion line may have a lower toughness than the SAW. It was found that the fusion line, Ji. was greater than the SAW toughness but much less than the base metal. Of greater importance may be that the crack growth resistance (JD-R) of the fusion line appeared to reach a steady-state value, while the SAW had a continually increasing JD-R curve. This explains why the cracks eventually turn to the fusion line in the pipe experiments. A method of incorporating these results would be to use the weld metal J-R curve up to the fusion-line steady-state J value. These results may be more important to LBB analyses than the ASME flaw evaluation procedures, since there is more crack growth with through-wall cracks in LBB analyses than for surface cracks in pipe flaw evaluations.

  14. Quantum metamaterials: Electromagnetic waves in a Josephson qubit line

    E-Print Network [OSTI]

    A. L. Rakhmanov; A. M. Zagoskin; Sergey Savel'ev; Franco Nori

    2007-12-19

    We consider the propagation of a classical electromagnetic wave through a transmission line, formed by identical superconducting charge qubits inside a superconducting resonator. Since the qubits can be in a coherent superposition of quantum states, we show that such a system demonstrates interesting new effects, such as a ``breathing'' photonic crystal with an oscillating bandgap, and a ``quantum Archimedean screw'' that transports, at an arbitrary controlled velocity, Josephson plasma waves through the transmission line. The key ingredient of these effects is that the optical properties of the Josephson transmission line are controlled by the quantum coherent state of the qubits.

  15. Deficiency of Broad Line AGNs in Compact Groups of Galaxies

    E-Print Network [OSTI]

    M. A. Martinez; A. del Olmo; R. Coziol; P. Focardi

    2008-03-25

    Based on a new survey of AGN activity in Compact Groups of Galaxies, we report a remarkable deficiency of Broad Line AGNs as compared to Narrow Line AGNs. The cause of such deficiency could be related to the average low luminosity of AGNs in CGs: $10^{39}$ erg s$^{-1}$. This result may imply lower accretion rates in CG AGNs, making Broad Line Regions (BLR) undetectable, or may indicate a genuine absence of BLRs. Both phenomena are consistent with gas stripping through tidal interaction and dry mergers.

  16. Image-line voltage controlled oscillators and grating antennas 

    E-Print Network [OSTI]

    Kirk, Alexander MacDonald

    1991-01-01

    Chair of Advisory Committee: Dr. Kai Chang A novel active antenna is proposed with an image-line construction. An image-line Gunn diode oscillator utilizing a cap resonator, for frequency stability, is the source module for the antenna. A varactor... is added to the source module for frequency tuning. Combining Gunn diode bias and varactor tuning, 2 percent electronic tuning bandwidth in the X-band has been achieved. The source module is an integral part of an image-line periodic grating antenna...

  17. Substrate binding by a bacterial ABC transporter involved in polysaccharide export

    SciTech Connect (OSTI)

    Cuthbertson, Leslie; Kimber, Matthew S.; Whitfield, Chris (Guelph)

    2008-04-02

    ATP-binding-cassette (ABC) transporters are responsible for the export of a wide variety of cell-surface glycoconjugates in both Gram-positive and Gram-negative bacteria. These include the O-antigenic polysaccharide (O-PS) portion of lipopolysaccharide, a crucial virulence determinant in Gram-negative pathogens. O-PSs are synthesized by one of two fundamentally different pathways. Escherichia coli O serotypes O8 and O9a provide the prototype systems for studying O-PS export via ABC transporters. The transporter is composed of the transmembrane component Wzm and the nucleotide-binding component Wzt. Although the N-terminal domain of Wzt is a conventional ABC protein, the C-terminal domain of Wzt (C-Wzt) is a unique structural element that determines the specificity of the transporter for either the O8 or O9a O-PS. We show here that the two domains of Wzt can function when expressed as separate polypeptides; both are essential for export. In vitro, C-Wzt binds its cognate O-PS by recognizing a residue located at the nonreducing end of the polymer. The crystal structure of C-WztO9a is reported here and reveals a {beta} sandwich with an immunoglobulin-like topology that contains the O-PS-binding pocket. Substrate interactions with nucleotide-binding domains have been demonstrated in an ABC exporter previously. However, to our knowledge substrate binding by a discrete, cytoplasmic accessory domain in an extended nucleotide-binding domain polypeptide has not previously been demonstrated. Elucidation of the substrate-recognition system involved in O-PS export provides insight into the mechanism that coordinates polymer biosynthesis, termination, and export.

  18. Binding of undamaged double stranded DNA to vaccinia virus uracil-DNA glycosylase

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Schormann, Norbert; Banerjee, Surajit; Ricciardi, Robert; Chattopadhyay, Debasish

    2015-06-02

    Background: Uracil-DNA glycosylases are evolutionarily conserved DNA repair enzymes. However, vaccinia virus uracil-DNA glycosylase (known as D4), also serves as an intrinsic and essential component of the processive DNA polymerase complex during DNA replication. In this complex D4 binds to a unique poxvirus specific protein A20 which tethers it to the DNA polymerase. At the replication fork the DNA scanning and repair function of D4 is coupled with DNA replication. So far, DNA-binding to D4 has not been structurally characterized. Results: This manuscript describes the first structure of a DNA-complex of a uracil-DNA glycosylase from the poxvirus family. This alsomore »represents the first structure of a uracil DNA glycosylase in complex with an undamaged DNA. In the asymmetric unit two D4 subunits bind simultaneously to complementary strands of the DNA double helix. Each D4 subunit interacts mainly with the central region of one strand. DNA binds to the opposite side of the A20-binding surface on D4. In comparison of the present structure with the structure of uracil-containing DNA-bound human uracil-DNA glycosylase suggests that for DNA binding and uracil removal D4 employs a unique set of residues and motifs that are highly conserved within the poxvirus family but different in other organisms. Conclusion: The first structure of D4 bound to a truly non-specific undamaged double-stranded DNA suggests that initial binding of DNA may involve multiple non-specific interactions between the protein and the phosphate backbone.« less

  19. E. coli SSB tetramer binds the rst and second molecules of (dT)35 with heat capacities of opposite sign

    E-Print Network [OSTI]

    Lohman, Timothy M.

    E. coli SSB tetramer binds the rst and second molecules of (dT)35 with heat capacities of opposite of the binding heat capacity ( CP). Here we examine SSB binding to shorter oligodeoxynucleotides ((dX)35) to probe whether this effect requires binding of one or two (dX)35 molecules per SSB tetramer. We nd

  20. Origami DNA model Mountain fold

    E-Print Network [OSTI]

    Csürös, Miklós

    Origami DNA model Mountain fold Solid lines are "mountains" and are to be folded away from you with the peak pointing towards you. 1. Fold all solid lines going lengthwise down the page into "mountain folds fold 2. Fold all dashed lines going lengthwise down the page into "valley folds". Mountain folds along

  1. DomainSpecific Runtime Variability In Product Line Architectures

    E-Print Network [OSTI]

    Zdun, Uwe

    of a set of products within a specific organization. Commonalities can be implemented in prefabricated from a Multimedia Home Platform product line: end­user personalization across different new media

  2. Domain-Specific Runtime Variability In Product Line Architectures

    E-Print Network [OSTI]

    Zdun, Uwe

    of a set of products within a specific organization. Commonalities can be implemented in prefabricated from a Multimedia Home Platform product line: end-user personalization across different new media

  3. Guide to good practices for line and training manager activities

    SciTech Connect (OSTI)

    1998-06-01

    The purpose of this guide is to provide direction for line and training managers in carrying out their responsibilities for training and qualifying personnel and to verify that existing training activities are effective.

  4. A mycological assessment of highly digestible protein sorghum lines

    E-Print Network [OSTI]

    Portillo, Ostilio Rolando

    2009-05-15

    The improved protein digestibility of the highly digestible protein (HD) sorghum lines is attributed to the invaginated shape of the endosperm protein bodies that provides better proteolytic access to the kafirins containing protein bodies. Recent...

  5. Multilevel Analysis in R with a Broken-line Example

    E-Print Network [OSTI]

    Leeuw, Jan de

    2008-01-01

    conduction velocity",col="RED ",lwd=3,main="FIML") for (i ini]+2*vml[i]),col="GREEN" ,lwd=3) ymllwd=3) for (i in 1:7) lines(c(

  6. Multilevel Analysis in R with a Broken-line Example

    E-Print Network [OSTI]

    Jan de Leeuw

    2011-01-01

    conduction velocity",col="RED ",lwd=3,main="FIML") for (i ini]+2*vml[i]),col="GREEN" ,lwd=3) ymllwd=3) for (i in 1:7) lines(c(

  7. Emission Lines and the Spectral Energy Distributions of Quasars

    E-Print Network [OSTI]

    B. J. Wilkes; P. J. Green; S. Mathur; J. C. McDowell

    1996-08-05

    Many years of study have failed to conclusively establish relations between a quasar's spectral energy distribution (SED) and the emission lines it is thought to produce. This is at least partially due to the lack of well-observed SEDs. We present initial results from a line--SED study for a sample of 43 quasars and active galaxies for which we have optical and ultra-violet spectra and far-infrared--X-ray SEDs. We present the results of tests for correlations between line equivalent widths and SED luminosity and slope parameters and compare these results to those from earlier studies. We find that the Baldwin effect is weaker when the luminosity is defined close to the ionising continuum of that line and conclude that the detailed SED is likely to be important in making further progress.

  8. EIS-0106: Great Falls-Conrad Transmission Line Project, Montana

    Broader source: Energy.gov [DOE]

    The Western Area Power Administration prepared this EIS to evaluate the environmental impacts of the construction and operation of a 230-kilovolt transmission line from Great Falls, Montana, to Conrad, Montana.

  9. Request for Proposals for New or Upgraded Transmission Line Projects...

    Office of Environmental Management (EM)

    Proposals for New or Upgraded Transmission Line Projects Under Section 1222 of the Energy Policy Act of 2005: Federal Register Notice Volume 75, No. 111 - Jun 10, 2012 Request for...

  10. Observation of interference fringes in Autler-Townes line shapes 

    E-Print Network [OSTI]

    Wilkinson, S. R.; Smith, A. V.; Scully, Marlan O.; Fry, Edward

    1996-01-01

    We observe Ramsey fringes in the absorption line shape for a weak optical field probing the transition between an unperturbed quantum state and one that is dynamically Stark shifted by a strong pump field....

  11. Single transmission line interrogated multiple channel data acquisition system

    DOE Patents [OSTI]

    Fasching, George E. (Morgantown, WV); Keech, Jr., Thomas W. (Morgantown, WV)

    1980-01-01

    A single transmission line interrogated multiple channel data acquisition system is provided in which a plurality of remote station/sensor circuits each monitors a specific process variable and each transmits measurement values over a single transmission line to a master interrogating station when addressed by said master interrogating station. Typically, as many as 330 remote stations may be parallel connected to the transmission line which may exceed 7,000 feet. The interrogation rate is typically 330 stations/second. The master interrogating station samples each station according to a shared, charging transmit-receive cycle. All remote station address signals, all data signals from the remote stations/sensors and all power for all of the remote station/sensors are transmitted via a single continuous terminated coaxial cable. A means is provided for periodically and remotely calibrating all remote sensors for zero and span. A provision is available to remotely disconnect any selected sensor station from the main transmission line.

  12. Evaluation of short-day onion doubled haploid lines 

    E-Print Network [OSTI]

    Walker, Ryan Lee

    2009-05-15

    Molecular marker analysis of seven putative onion (Allium cepa) doubled haploid (DH) lines developed at Texas A&M University was conducted to verify genetic homozygosity. Analysis was also conducted on five equivalent ...

  13. Federal Line Management Oversight of Department of Energy Nuclear Facilities

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2013-04-04

    The Guide was developed in support of DOE O 226.1B to provide guidance that may be useful to DOE line management organizations in meeting the provisions of that order when applied to nuclear facilities.

  14. Wireless Sensor Network for Electric Transmission Line Monitoring

    SciTech Connect (OSTI)

    Alphenaar, Bruce

    2009-06-30

    Generally, federal agencies tasked to oversee power grid reliability are dependent on data from grid infrastructure owners and operators in order to obtain a basic level of situational awareness. Since there are many owners and operators involved in the day-to-day functioning of the power grid, the task of accessing, aggregating and analyzing grid information from these sources is not a trivial one. Seemingly basic tasks such as synchronizing data timestamps between many different data providers and sources can be difficult as evidenced during the post-event analysis of the August 2003 blackout. In this project we investigate the efficacy and cost effectiveness of deploying a network of wireless power line monitoring devices as a method of independently monitoring key parts of the power grid as a complement to the data which is currently available to federal agencies from grid system operators. Such a network is modeled on proprietary power line monitoring technologies and networks invented, developed and deployed by Genscape, a Louisville, Kentucky based real-time energy information provider. Genscape measures transmission line power flow using measurements of electromagnetic fields under overhead high voltage transmission power lines in the United States and Europe. Opportunities for optimization of the commercial power line monitoring technology were investigated in this project to enable lower power consumption, lower cost and improvements to measurement methodologies. These optimizations were performed in order to better enable the use of wireless transmission line monitors in large network deployments (perhaps covering several thousand power lines) for federal situational awareness needs. Power consumption and cost reduction were addressed by developing a power line monitor using a low power, low cost wireless telemetry platform known as the ''Mote''. Motes were first developed as smart sensor nodes in wireless mesh networking applications. On such a platform, it has been demonstrated in this project that wireless monitoring units can effectively deliver real-time transmission line power flow information for less than $500 per monitor. The data delivered by such a monitor has during the course of the project been integrated with a national grid situational awareness visualization platform developed by Oak Ridge National Laboratory. Novel vibration energy scavenging methods based on piezoelectric cantilevers were also developed as a proposed method to power such monitors, with a goal of further cost reduction and large-scale deployment. Scavenging methods developed during the project resulted in 50% greater power output than conventional cantilever-based vibrational energy scavenging devices typically used to power smart sensor nodes. Lastly, enhanced and new methods for electromagnetic field sensing using multi-axis magnetometers and infrared reflectometry were investigated for potential monitoring applications in situations with a high density of power lines or high levels of background 60 Hz noise in order to isolate power lines of interest from other power lines in close proximity. The goal of this project was to investigate and demonstrate the feasibility of using small form factor, highly optimized, low cost, low power, non-contact, wireless electric transmission line monitors for delivery of real-time, independent power line monitoring for the US power grid. The project was divided into three main types of activity as follows; (1) Research into expanding the range of applications for non-contact power line monitoring to enable large scale low cost sensor network deployments (Tasks 1, 2); (2) Optimization of individual sensor hardware components to reduce size, cost and power consumption and testing in a pilot field study (Tasks 3,5); and (3) Demonstration of the feasibility of using the data from the network of power line monitors via a range of custom developed alerting and data visualization applications to deliver real-time information to federal agencies and others tasked with grid reliability (Tasks 6,8)

  15. Microsoft Word - News Release -- Annual Reports now on line 2...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    20, 2015 CONTACT: Mike Hansen, BPA 503-230-4328 or 503-230-5131 All 77 of BPA's annual reports now on line Portland, Ore. - The Bonneville Power Administration now has all 77 of...

  16. Oxford Colleges On-line Reports for Tutorials

    E-Print Network [OSTI]

    Wallace, Mark

    OxCORT Oxford Colleges On-line Reports for Tutorials Tutorial Office Role Version 4.4 BSP Training Overview of Tutorial reports.................................................................11 Overview of Revision Class reports.....................................................12 Overview of payments

  17. Oxford Colleges On-line Reports for Tutorials

    E-Print Network [OSTI]

    Wallace, Mark

    OxCORT Oxford Colleges On-line Reports for Tutorials Organising or Personal Tutor Role Version 4 of Tutorial reports...................................................................8 Overview of Revision Class reports.......................................................8 Overview of payments

  18. Oxford Colleges On-line Reports for Tutorials

    E-Print Network [OSTI]

    Wallace, Mark

    OxCORT Oxford Colleges On-line Reports for Tutorials Senior Tutor Role Version 4.4 BSP Training of Tutorial reports...................................................................8 Overview of Revision Class reports.......................................................8 Overview of payments

  19. Oxford Colleges On-line Reports for Tutorials

    E-Print Network [OSTI]

    Wallace, Mark

    OxCORT Oxford Colleges On-line Reports for Tutorials Tutor Role Version 4.5 BSP Training Team Overview of Tutorial reports...................................................................8 Overview of Revision Class reports.......................................................8 Report states

  20. On-line hydraulic state prediction for water distribution systems

    E-Print Network [OSTI]

    Whittle, Andrew

    This paper describes and demonstrates a method for on?line hydraulic state prediction in urban water networks. The proposed method uses a Predictor?Corrector (PC) approach in which a statistical data?driven algorithm is ...

  1. Soliton communication lines based on spectrally efficient modulation formats

    SciTech Connect (OSTI)

    Yushko, O V; Redyuk, A A

    2014-06-30

    We report the results of mathematical modelling of optical-signal propagation in soliton fibre-optic communication lines (FOCLs) based on spectrally efficient signal modulation formats. We have studied the influence of spontaneous emission noise, nonlinear distortions and FOCL length on the data transmission quality. We have compared the characteristics of a received optical signal for soliton and conventional dispersion compensating FOCLs. It is shown that in the presence of strong nonlinearity long-haul soliton FOCLs provide a higher data transmission performance, as well as allow higher order modulation formats to be used as compared to conventional communication lines. In the context of a coherent data transmission, soliton FOCLs allow the use of phase modulation with many levels, thereby increasing the spectral efficiency of the communication line. (optical communication lines)

  2. Principal stress line computation for discrete topology design

    E-Print Network [OSTI]

    Tam, Kam-Ming Mark

    2015-01-01

    Principal stress-lines are pairs of orthogonal curves that indicate trajectories of internal forces. Subsequently, these curves idealize paths of material continuity, and naturally encode the optimal topology for any ...

  3. Comparison of Tamspan 90 peanut component lines for aflatoxin production 

    E-Print Network [OSTI]

    Lopez, Yolanda

    1994-01-01

    The 38 lines that comprise 'Tamspan 90' peanut were studied for differential Aspet*llu,s flavus Links and A. parasiticus Spear growth and aflatoxin production under field and laboratory conditions. Required environmental conditions of temperature...

  4. A framework for on-line simulation systems 

    E-Print Network [OSTI]

    Drake, Glenn Richardson

    1996-01-01

    Simulation has traditionally been used for off-line planning and design. The advent of computer integrated manufacturing and flexible manufacturing systems has increased interest in shop floor control systems that use ...

  5. Projective lines over one-dimensional semilocal domains

    E-Print Network [OSTI]

    2013-01-21

    contracts to a maximal ideal in R[y] or in R[y?1] implies that R is Henselian. Moti- vated by this question, we consider the structure of the projective line Proj(R[s, ...

  6. CRAD, NNSA- Evaluation Site Office Line Oversight Programs

    Broader source: Energy.gov [DOE]

    CRAD for Evaluation Site Office Line Oversight Programs. Criteria Review and Approach Documents (CRADs) that can be used to conduct a well-organized and thorough assessment of elements of safety and health programs.

  7. A STUDY OF ION LINE BROADENING IN THE TORMAC DISCHARGE.

    E-Print Network [OSTI]

    Shaw, Robert Stephen

    2010-01-01

    of the main confinement magnetic field is not maintained fordrag. Magnetic probes indicate that the confinement fieldmagnetic field line configurations, such as the magnetic mirror, do not have this B limitation. However, particle confinement

  8. COLLECTED TRANSMISSION-LINE IDENTITIES Matthew A. Morgan

    E-Print Network [OSTI]

    Groppi, Christopher

    -line identities are a useful tool in the early stages of passive network design, and many such identities are well, and Coupling Structures, Artech House, Norwood MA. 1980. [4] R. Wenzel, "Exact design of TEM microwave networks

  9. Line and Net Pattern Segmentation Using Shape Modeling Adam Huanga

    E-Print Network [OSTI]

    : Image processing, image enhancement, image segmentation, feature extraction, matched filter, crest lines of blood vessels in retinal images: 1) Blood vessels usually have small curvatures and can be approximated

  10. Internal-integral sodium return line for sodium heat engine

    DOE Patents [OSTI]

    Hunt, Thomas K. (Ann Arbor, MI)

    1985-01-01

    A thermoelectric generator device which converts heat energy to electrical energy. An alkali metal is used with a solid electrolyte and a portion of the return line for the alkali metal is located within the generator vacuum space.

  11. Federal Line Management Oversight of Department of Energy Nuclear Facilities

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2011-04-25

    The purpose of this Guide is to provide U.S. Department of Energy (DOE) line management with guidance that may be useful to them in effectively and efficiently implementing the requirements of DOE O 226.1B, Implementation of Department of Energy Oversight Policy, date April 25, 2011, as applied to Federal line management of hazard category 1, 2, and 3 nuclear facilities.

  12. LLNL line-item construction projects Master Site Plan

    SciTech Connect (OSTI)

    1996-04-15

    This interim submittal is an updated 1996 overview of the Master Plan based on the 1995 LLNL Site Development Plan, illustrating the future land use considerations, and the locations of proposed facilities as documented through the line item development process and keyed to the summary table. The following components in addition to the line-item proposals remain key elements in the implementation strategy of the Master Plan: personnel migration, revitalization, space reduction, classified core contraction, utility systems, and environmental restoration.

  13. Volume painting: incorporating volumetric rendering with line integral convolution (LIC) 

    E-Print Network [OSTI]

    Lee, Jaewook

    2005-11-01

    -1 VOLUME PAINTING: INCORPORATING VOLUMETRIC RENDERING WITH LINE INTEGRAL CONVOLUTION (LIC) A Thesis by JAEWOOK LEE Submitted to the Office of Graduate Studies of Texas A&M University in partial fulfillment of the requirements for the degree of MASTER... Painting: Incorporating Volumetric Rendering with Line Integral Convolution (LIC). (August 2005) Jaewook Lee, B.F.A., Pusan National University Chair of Advisory Committee: Dr. Ergun Akleman This thesis presents an expressive (non-photorealistic) rendering...

  14. The 2mrad Crossing Angle Interaction Region and Extraction Line

    SciTech Connect (OSTI)

    Appleby, R.; U., Manchester; Angal-Kalinin, D.; Dadoun, O.; Bambade, P.; Parker, B.; Keller, L.; Moffeit, K.; Nosochkov, Y.; Seryi, A.; Spencer, C.; Carter, J.; Royal Holloway, U.of London; Napoly, O.; /DAPNIA, Saclay

    2006-07-12

    A complete optics design for the 2mrad crossing angle interaction region and extraction line was presented at Snowmass 2005. Since this time, the design task force has been working on developing and improving the performance of the extraction line. The work has focused on optimizing the final doublet parameters and on reducing the power losses resulting from the disrupted beam transport. In this paper, the most recent status of the 2mrad layout and the corresponding performance are presented.

  15. Corrugated outer sheath gas-insulated transmission line

    DOE Patents [OSTI]

    Kemeny, George A. (Pittsburgh, PA); Cookson, Alan H. (Churchill Boro, PA)

    1981-01-01

    A gas-insulated transmission line includes two transmission line sections each of which are formed of a corrugated outer housing enclosing an inner high-voltage conductor disposed therein, with insulating support means supporting the inner conductor within the outer housing and an insulating gas providing electrical insulation therebetween. The outer housings in each section have smooth end sections at the longitudinal ends thereof which are joined together by joining means which provide for a sealing fixed joint.

  16. Gas insulated transmission line having tapered particle trapping ring

    DOE Patents [OSTI]

    Cookson, Alan H. (Pittsburgh, PA)

    1982-01-01

    A gas-insulated transmission line includes an outer sheath, an inner conductor, insulating supports and an insulating gas. A particle-trapping ring is secured to each insulating support, and it is comprised of a central portion and two tapered end portions. The ends of the particle trapping ring have a smaller diameter than the central portion of the ring, so as to enable the use of the particle trapping ring in a curved transmission line.

  17. Determination of age in forensic dentistry from cemental incremental lines 

    E-Print Network [OSTI]

    Sousa, Eliane Marques Duarte de

    1987-01-01

    ) John E. Martin (Member) H. Del Var Petersen (Member) Gerald R. Bratton (Head of Department) December lg87 ABSTNACT Determination of Age in Forensic Dentistry from Cemental Incremental Lines. (December 1987) Eliane Marques Duarte de Sousa, D. D...DETERMINATION OF AGE IN FORENSIC DENTISTRY FROM CEMENTAL INCREMENTAL LINES Thes' s by ELIANE MARJUES DLARTE DF SOUSA Submitted to the Graduate College of Texas A?M Univer ity ' n r. srt' al f"lf' llment of the requirements for the degree...

  18. The Broad Line Region of AGN: Kinematics and Physics

    E-Print Network [OSTI]

    L. C. Popovic

    2006-12-23

    In this paper a discussion of kinematics and physics of the Broad Line Region (BLR) is given. The possible physical conditions in the BLR and problems in determination of the physical parameters (electron temperature and density) are considered. Moreover, one analyses the geometry of the BLR and the probability that (at least) a fraction of the radiation in the Broad Emission Lines (BELs) originates from a relativistic accretion disk.

  19. Beyond the detergent effect: a binding site for sodium dodecyl sulfate (SDS) in mammalian apoferritin

    SciTech Connect (OSTI)

    Liu, Renyu Bu, Weiming; Xi, Jin; Mortazavi, Shirin R.; Cheung-Lau, Jasmina C.; Dmochowski, Ivan J.; Loll, Patrick J.

    2012-05-01

    Using X-ray crystallography and isothermal titration calorimetry, we show that sodium dodecyl sulfate (SDS) binds specifically to a pre-formed internal cavity in horse-spleen apoferritin. Although sodium dodecyl sulfate (SDS) is widely used as an anionic detergent, it can also exert specific pharmacological effects that are independent of the surfactant properties of the molecule. However, structural details of how proteins recognize SDS are scarce. Here, it is demonstrated that SDS binds specifically to a naturally occurring four-helix bundle protein: horse apoferritin. The X-ray crystal structure of the apoferritin–SDS complex was determined at a resolution of 1.9 Å and revealed that the SDS binds in an internal cavity that has previously been shown to recognize various general anesthetics. A dissociation constant of 24 ± 9 µM at 293 K was determined by isothermal titration calorimetry. SDS binds in this cavity by bending its alkyl tail into a horseshoe shape; the charged SDS head group lies in the opening of the cavity at the protein surface. This crystal structure provides insights into the protein–SDS interactions that give rise to binding and may prove useful in the design of novel SDS-like ligands for some proteins.

  20. Protein binding studies of technetium-99m-labeled phosphine and isocyanide cationic complexes

    SciTech Connect (OSTI)

    Zanelli, G.D.; Cook, N.; Lahiri, A.; Ellison, D.; Webbon, P.; Woolley, G.

    1988-01-01

    Most /sup 99m/Tc/phosphine/isocyanide complexes synthesized to date show preferential uptake by the myocardium of many animal species but not in man. A new complex has been synthesized, (/sup 99m/Tc(DEPE)2(CNR)2), +(DEPIC), where R = t - butyl isocyanide, which in three animal species images the myocardium very well, but in humans it remains primarily in the blood pool. One reason for the difference in the behavior of these complexes in different species could be the characteristics of their binding to plasma proteins. The protein binding characteristics of DEPIC and two other well-known complexes have therefore been studied. Whereas the other complexes bind nonspecifically to many proteins both in animal and human plasma, DEPIC binds almost exclusively to prealbumin in humans but nonspecifically to other proteins in the rabbit. The binding sites in human plasma appear to be those normally occupied by thyroxine on the prealbumin tetramer and these can be blocked by sodium salicylate.

  1. Advancing along the N = Z line : a 30 year odyssey.

    SciTech Connect (OSTI)

    Lister, C. J.; Physics

    2008-01-01

    N = Z nuclei have always had a special role in nuclear physics due to the symmetry of their wavefunctions, and their anomalously high binding energy. Beyond {sup 56}Ni they gain new significance, as they lie close to the proton dripline, and approaching {sup 100}Sn, they define the dripline. They are interesting for their structure, for rp-process nucleosynthesis, and as laboratories for weak interaction physics. In this paper I review some currently interesting topics and open questions.

  2. Ultraviolet Diagnostics for the Emission Line Gas in Active Galaxies

    E-Print Network [OSTI]

    M. G. Allen; M. A. Dopita; Z. I. Tsvetanov

    1998-01-06

    Optical diagnostic diagrams are frequently ambiguous as a test of the photoionization or fast shock models of the narrow line regions of active galaxies. Here, we present a set of UV line ratio diagrams which can discriminate between pure shock and photoionization modes of excitation, and to some extent, also discriminate shocks with ionized precursors from photoionization. These diagrams use relatively bright emission lines and reddening insensitive ratios and provide a practical observational test for separating the excitation mechanisms of the narrow line regions of active galaxies. The most useful diagrams are those involving the various ionization stages of Carbon, [OIII]5007/H-beta vs. CIV 1550/ HeII 1640 and the purely UV ratio pair CII] 2326 / CIII] 1909 vs. CIV 1550 / CIII]909. Temperature sensitive FUV lines CIII 977 and NIII 991 also provide good discriminants. The models are compared to observations of nearby AGN, and also to high redshift objects where the UV lines are shifted into the optical.

  3. Solving radiative transfer with line overlaps using Gauss Seidel algorithms

    E-Print Network [OSTI]

    F. Daniel; J. Cernicharo

    2008-07-11

    The improvement in observational facilities requires refining the modelling of the geometrical structures of astrophysical objects. Nevertheless, for complex problems such as line overlap in molecules showing hyperfine structure, a detailed analysis still requires a large amount of computing time and thus, misinterpretation cannot be dismissed due to an undersampling of the whole space of parameters. We extend the discussion of the implementation of the Gauss--Seidel algorithm in spherical geometry and include the case of hyperfine line overlap. We first review the basics of the short characteristics method that is used to solve the radiative transfer equations. Details are given on the determination of the Lambda operator in spherical geometry. The Gauss--Seidel algorithm is then described and, by analogy to the plan--parallel case, we see how to introduce it in spherical geometry. Doing so requires some approximations in order to keep the algorithm competitive. Finally, line overlap effects are included. The convergence speed of the algorithm is compared to the usual Jacobi iterative schemes. The gain in the number of iterations is typically factors of 2 and 4 for the two implementations made of the Gauss--Seidel algorithm. This is obtained despite the introduction of approximations in the algorithm. A comparison of results obtained with and without line overlaps for N2H+, HCN, and HNC shows that the J=3-2 line intensities are significantly underestimated in models where line overlap is neglected.

  4. Optical emission line monitor with background observation and cancellation

    DOE Patents [OSTI]

    Goff, David R. (Star City, WV); Notestein, John E. (Morgantown, WV)

    1986-01-01

    A fiber optics based optical emission line monitoring system is provided in which selected spectral emission lines, such as the sodium D-line emission in coal combustion, may be detected in the presence of interferring background or blackbody radiation with emissions much greater in intensity than that of the emission line being detected. A bifurcated fiber optic light guide is adapted at the end of one branch to view the combustion light which is guided to a first bandpass filter, adapted to the common trunk end of the fiber. A portion of the light is reflected back through the common trunk portion of the fiber to a second bandpass filter adapted to the end of the other branch of the fiber. The first filter bandpass is centered at a wavelength corresponding to the emission line to be detected with a bandwidth of about three nanometers (nm). The second filter is centered at the same wavelength but having a width of about 10 nm. First and second light detectors are located to view the light passing through the first and second filters respectively. Thus, the second detector is blind to the light corresponding to the emission line of interest detected by the first detector and the difference between the two detector outputs is uniquely indicative of the intensity of only the combustion flame emission of interest. This instrument can reduce the effects of interferring blackbody radiation by greater than 20 dB.

  5. Optical emission line monitor with background observation and cancellation

    DOE Patents [OSTI]

    Goff, D.R.; Notestein, J.E.

    1985-01-04

    A fiber optics based optical emission line monitoring system is provided in which selected spectral emission lines, such as the sodium D-line emission in coal combustion, may be detected in the presence of interferring background or blackbody radiation with emissions much greater in intensity than that of the emission line being detected. A bifurcated fiber optic light guide is adapted at the end of one branch to view the combustion light which is guided to a first bandpass filter, adapted to the common trunk end of the fiber. A portion of the light is reflected back through the common trunk portion of the fiber to a second bandpass filter adapted to the end of the other branch of the fiber. The first filter bandpass is centered at a wavelength corresponding to the emission line to be detected with a bandwidth of about three nanometers (nm). The second filter is centered at the same wavelength but having a width of about 10 nm. First and second light detectors are located to view the light passing through the first and second filters respectively. Thus, the second detector is blind to the light corresponding to the emission line of interest detected by the first detector and the difference between the two detector outputs is uniquely indicative of the intensity of only the combustion flame emission of interest. This instrument can reduce the effects of interfering blackbody radiation by greater than 20 dB.

  6. The fine structure line deficit in S 140

    E-Print Network [OSTI]

    Ossenkopf, Volker; Okada, Yoko; Mookerjea, Bhaswati; van der Tak, Floris F S; Simon, Robert; Pütz, Patrick; Güsten, Rolf

    2015-01-01

    We try to understand the gas heating and cooling in the S 140 star forming region by spatially and spectrally resolving the distribution of the main cooling lines with GREAT/SOFIA. We mapped the fine structure lines of [OI] (63 {\\mu}m) and [CII] (158 {\\mu}m) and the rotational transitions of CO 13-12 and 16-15 with GREAT/SOFIA and analyzed the spatial and velocity structure to assign the emission to individual heating sources. We measure the optical depth of the [CII] line and perform radiative transfer computations for all observed transitions. By comparing the line intensities with the far-infrared continuum we can assess the total cooling budget and measure the gas heating efficiency. The main emission of fine structure lines in S 140 stems from a 8.3'' region close to the infrared source IRS 2 that is not prominent at any other wavelength. It can be explained by a photon-dominated region (PDR) structure around the embedded cluster if we assume that the [OI] line intensity is reduced by a factor seven due ...

  7. Structures of Adnectin/Protein Complexes Reveal an Expanded Binding Footprint

    SciTech Connect (OSTI)

    Ramamurthy, Vidhyashankar; Krystek, Jr., Stanley R.; Bush, Alexander; Wei, Anzhi; Emanuel, Stuart L.; Gupta, Ruchira Das; Janjua, Ahsen; Cheng, Lin; Murdock, Melissa; Abramczyk, Bozena; Cohen, Daniel; Lin, Zheng; Morin, Paul; Davis, Jonathan H.; Dabritz, Michael; McLaughlin, Douglas C.; Russo, Katie A.; Chao, Ginger; Wright, Martin C.; Jenny, Victoria A.; Engle, Linda J.; Furfine, Eric; Sheriff, Steven

    2014-10-02

    Adnectins are targeted biologics derived from the tenth type III domain of human fibronectin ({sup 10}Fn3), a member of the immunoglobulin superfamily. Target-specific binders are selected from libraries generated by diversifying the three {sup 10}Fn3 loops that are analogous to the complementarity determining regions of antibodies. The crystal structures of two Adnectins were determined, each in complex with its therapeutic target, EGFR or IL-23. Both Adnectins bind different epitopes than those bound by known monoclonal antibodies. Molecular modeling suggests that some of these epitopes might not be accessible to antibodies because of the size and concave shape of the antibody combining site. In addition to interactions from the Adnectin diversified loops, residues from the N terminus and/or the {beta} strands interact with the target proteins in both complexes. Alanine-scanning mutagenesis confirmed the calculated binding energies of these {beta} strand interactions, indicating that these nonloop residues can expand the available binding footprint.

  8. Beta-endorphin and alpha-n-acetyl beta-endorphin; synthesis, conformation and binding parameter

    SciTech Connect (OSTI)

    Lovegren, E.S.

    1986-01-01

    Beta-endorphin (EP) is a 31-residue opioid peptide found in many tissues, including the pituitary, brain and reproductive tract. Alpha-amino-acetyl beta-endorphin (AcEP) was characterized spectroscopically by proton nuclear magnetic resonance (NMR) and circular dichroism in deuterated water and trifluoroethanol (TFE). Both EP and AcEP bind to neuroblastoma N2a cells. This binding was not mediated through opiate receptors, and both peptides seemed to bind at common sites. Ovarian immunoreactive-EP levels were determined for immature and mature rates. These levels were found to be responsive to exogenous gonadotropin treatment in immature animals. A large percentage of the immunoreactive-EP is present in follicular fluid, and most of the endorphin-like peptides were acetylated, as measured by radioimmunoassay. Chromatogaphic analysis suggested at least three EP-like species: EP, a carboxy-terminally cleaved and an amino-terminally acetylated EP.

  9. Theory of the colossal Van-der-Waals binding in soft and hard condensed matter

    E-Print Network [OSTI]

    Mladen Georgiev; Alexander Gochev; Jai Singh

    2005-10-12

    A simple theory is proposed for the dispersive molecular binding of unusually high magnitude due to an enhanced polarizability. Two alternative ways have so far been considered in the literature leading to the polarizability enhancement: (i) a vibronic energy level gap narrowing, as proposed by us with regard to a hypothetical exciton matter, and (ii) a giant electric dipole in a Rydberg state of constituent atoms, as proposed by Gilman with regard to an enigmatic substance building the ball lightning. We now combine the two mechanisms to obtain concrete expressions for the colossal binding energy. The problem is exemplified for a three-level system coupled to the umbrella mode of an ammonia molecule. Other possibilities for the design of enhanced-polarizability molecules are also discussed. The colossal Van-der-Waals binding is most likely to materialize in hard condensed matter and perhaps less so in soft condensed matter.

  10. LINC Complexes Form by Binding of Three KASH Peptides to Domain Interfaces of Trimeric SUN Proteins

    SciTech Connect (OSTI)

    Sosa, Brian A.; Rothballer, Andrea; Kutay, Ulrike; Schwartz, Thomas U.

    2012-08-31

    Linker of nucleoskeleton and cytoskeleton (LINC) complexes span the nuclear envelope and are composed of KASH and SUN proteins residing in the outer and inner nuclear membrane, respectively. LINC formation relies on direct binding of KASH and SUN in the perinuclear space. Thereby, molecular tethers are formed that can transmit forces for chromosome movements, nuclear migration, and anchorage. We present crystal structures of the human SUN2-KASH1/2 complex, the core of the LINC complex. The SUN2 domain is rigidly attached to a trimeric coiled coil that prepositions it to bind three KASH peptides. The peptides bind in three deep and expansive grooves formed between adjacent SUN domains, effectively acting as molecular glue. In addition, a disulfide between conserved cysteines on SUN and KASH covalently links both proteins. The structure provides the basis of LINC complex formation and suggests a model for how LINC complexes might arrange into higher-order clusters to enhance force-coupling.

  11. Crystal Structure of the HP1-EMSY Complex Reveals an Unusual Mode of HP1 Binding

    SciTech Connect (OSTI)

    Huang,Y.; Myers, M.; Xu, R.

    2006-01-01

    Heterochromatin protein-1 (HP1) plays an essential role in both the assembly of higher-order chromatin structure and epigenetic inheritance. The C-terminal chromo shadow domain (CSD) of HP1 is responsible for homodimerization and interaction with a number of chromatin-associated nonhistone proteins, including EMSY, which is a BRCA2-interacting protein that has been implicated in the development of breast and ovarian cancer. We have determined the crystal structure of the HP1{beta} CSD in complex with the N-terminal domain of EMSY at 1.8 Angstroms resolution. Surprisingly, the structure reveals that EMSY is bound by two HP1 CSD homodimers, and the binding sequences differ from the consensus HP1 binding motif PXVXL. This structural information expands our understanding of HP1 binding specificity and provides insights into interactions between HP1 homodimers that are likely to be important for heterochromatin formation.

  12. Evaluation of Two Fluorescent Dyes used in Immunofluorescent Microscopy for the Detection of Proteinaceous Binding Media in Wall Paintings

    E-Print Network [OSTI]

    de Alarcon, Tessa

    2012-01-01

    identify organic binding media within the individual paintorganic components, pigment binder interactions, but also possible variations in paintpaint layers. Spatially resolved analysis of organic

  13. Human Biliverdin Reductase Is a Leucine Zipper-like DNA-binding Protein and Functions in Transcriptional Activation of Heme

    E-Print Network [OSTI]

    Zulfiqar Ahmad

    BVR DNA complex formation; and (e) purified preparations of hBVR or hHO-1 do not bind to DNA with two AP-1

  14. Binding of formyl peptides to Walker 256 carcinosarcoma cells and the chemotactic response of these cells

    SciTech Connect (OSTI)

    Rayner, D.C.; Orr, F.W.; Shiu, R.P.

    1985-05-01

    N-Formylmethionylleucylphenylalanine (fMLP) induces chemotaxis in leukocytes, the response being mediated by peptide binding to a receptor on the plasma membrane. In tumor cells, this peptide has been reported to induce cellular swelling and chemotaxis in vitro and to enhance the localization of circulating tumor cells in vivo. In the Boyden chamber, the authors evaluated the migratory responses of Walker carcinosarcoma 256 cells to varying concentrations of fMLP. Sigmoidal dose-response curves were obtained with the dose of chemotactic factor that elicits a half-maximal chemotactic response of 5.0 +/- 2.5 X 10(-8) M. Checkerboard analysis indicated that these responses were dependent upon a concentration gradient of fMLP with increases in migration of circa 2 to 2.5 times that of random movement. To examine the binding of fMLP, the tumor cells were incubated with 5 X 10(-9) M fML-(/sup 3/H)P in Hanks balanced salt solution. Specific binding (0.5 to 1% of total radioligand, to whole cells inhibited by 5 X 10(-6) M fMLP) approached equilibrium after 4 to 6 h at 4 degrees C and after 6 to 10 h at 22 degrees C. Autoradiographic studies demonstrated heterogeneous binding of the peptide by tumor cells and also showed its intracellular localization. In homogenates of Walker cells prepared in 0.1 M Tris HCl, pH 7.4, with 10 mM MgCl2 and bovine serum albumin (1 mg/ml), specific binding of approximately 0.5% of total fML-(/sup 3/H)P reached equilibrium after 60 min at 4 degrees C. In whole cells and homogenates, binding was reversible by addition of unlabeled fMLP.

  15. Ligand-induced conformational changes in a thermophilic ribose-binding protein

    SciTech Connect (OSTI)

    Cuneo, Matthew J.; Beese, Lorena S.; Hellinga, Homme W.

    2009-05-21

    Members of the periplasmic binding protein (PBP) superfamily are involved in transport and signaling processes in both prokaryotes and eukaryotes. Biological responses are typically mediated by ligand-induced conformational changes in which the binding event is coupled to a hinge-bending motion that brings together two domains in a closed form. In all PBP-mediated biological processes, downstream partners recognize the closed form of the protein. This motion has also been exploited in protein engineering experiments to construct biosensors that transduce ligand binding to a variety of physical signals. Understanding the mechanistic details of PBP conformational changes, both global (hinge bending, twisting, shear movements) and local (rotamer changes, backbone motion), therefore is not only important for understanding their biological function but also for protein engineering experiments. Here we present biochemical characterization and crystal structure determination of the periplasmic ribose-binding protein (RBP) from the hyperthermophile Thermotoga maritima in its ribose-bound and unliganded state. The T. maritima RBP (tmRBP) has 39% sequence identity and is considerably more resistant to thermal denaturation (appTm value is 108 C) than the mesophilic Escherichia coli homolog (ecRBP) (appTm value is 56 C). Polar ligand interactions and ligand-induced global conformational changes are conserved among ecRBP and tmRBP; however local structural rearrangements involving side-chain motions in the ligand-binding site are not conserved. Although the large-scale ligand-induced changes are mediated through similar regions, and are produced by similar backbone movements in tmRBP and ecRBP, the small-scale ligand-induced structural rearrangements differentiate the mesophile and thermophile. This suggests there are mechanistic differences in the manner by which these two proteins bind their ligands and are an example of how two structurally similar proteins utilize different mechanisms to form a ligand-bound state.

  16. DETERMINING INTERSTELLAR CARBON ABUNDANCES FROM STRONG-LINE TRANSITIONS

    SciTech Connect (OSTI)

    Sofia, U. J. [Physics Department, American University, Washington, DC 20016 (United States); Parvathi, V. S.; Babu, B. R. S. [Physics Department, University of Calicut, Kerala (India); Murthy, J. [Indian Institute of Astrophysics, Kormangala, Bengaluru (India)

    2011-01-15

    Carbon is arguably the most important element in the interstellar medium, yet its abundance in gas and dust is poorly understood due to a paucity of data. We explore the possibility of substantially increasing our knowledge of interstellar carbon by applying and assessing a new method for determining the column density of the dominant ion of interstellar carbon in diffuse neutral lines of sight. The method relies on profile fitting of the strong transition of C II at 1334 A in spectra continuum normalized with stellar models. We apply our method to six sight lines for which the carbon abundance has previously been determined with a weak intersystem absorption transition. Our strong-line method consistently shows a significantly lower gas-phase C abundance than the measurements from the weak lines. This result implies that more carbon could reside in dust than was previously thought. This has implications for dust models, which often suffer from a lack of sufficient carbon to plausibly explain extinction. There is no immediately clear explanation for the difference found between the strong- and weak-line C II determinations, but there are indications that the results from the method presented here have advantages over the weak-line column densities. If this is the case, then the reported oscillator strength for the C II transition at 2325 A may be too small. Our findings further suggest that damping wings modeled with a single absorption component may not produce accurate abundances. This problem could affect a large number of H I abundances determined through absorption line analysis that are reported in the literature.

  17. Constraining fundamental constant evolution with HI and OH lines

    E-Print Network [OSTI]

    N. Kanekar; G. I. Langston; J. T. Stocke; C. L. Carilli; K. L. Menten

    2012-01-16

    We report deep Green Bank Telescope spectroscopy in the redshifted HI 21cm and OH 18cm lines from the $z = 0.765$ absorption system towards PMN J0134-0931. A comparison between the "satellite" OH 18cm line redshifts, or between the redshifts of the HI 21cm and "main" OH 18cm lines, is sensitive to changes in different combinations of three fundamental constants, the fine structure constant $\\alpha$, the proton-electron mass ratio $\\mu \\equiv m_p/m_e$ and the proton g-factor $g_p$. We find that the satellite OH 18cm lines are not perfectly conjugate, with both different line shapes and stronger 1612 MHz absorption than 1720 MHz emission. This implies that the satellite lines of this absorber are not suitable to probe fundamental constant evolution. A comparison between the redshifts of the HI 21cm and OH 18cm lines, via a multi-Gaussian fit, yields the strong constraint $[\\Delta F/F] = [-5.2 \\pm 4.3] \\times 10^{-6}$, where $F \\equiv g_p [\\mu \\alpha^2]^{1.57}$ and the error budget includes contributions from both statistical and systematic errors. We thus find no evidence for a change in the constants between $z = 0.765$ and the present epoch. Incorporating the constraint $[\\Delta \\mu/\\mu ] < 3.6 \\times 10^{-7}$ from another absorber at a similar redshift and assuming that fractional changes in $g_p$ are much smaller than those in $\\alpha$, we obtain $[\\Delta \\alpha/\\alpha ] = (-1.7 \\pm 1.4) \\times 10^{-6}$ over a lookback time of 6.7 Gyrs.

  18. Experimental Determination of In-Medium Cluster Binding Energies and Mott Points in Nuclear Matter

    E-Print Network [OSTI]

    K. Hagel; R. Wada; L. Qin; J. B. Natowitz; S. Shlomo; A. Bonasera; G. Röpke; S. Typel; Z. Chen; M. Huang; J. Wang; H. Zheng; S. Kowalski; C. Bottosso; M. Barbui; M. R. D. Rodrigues; K. Schmidt; D. Fabris; M. Lunardon; S. Moretto; G. Nebbia; S. Pesente; V. Rizzi; G. Viesti; M. Cinausero; G. Prete; T. Keutgen; Y. El Masri; Z. Majka

    2012-03-20

    In medium binding energies and Mott points for $d$, $t$, $^3$He and $\\alpha$ clusters in low density nuclear matter have been determined at specific combinations of temperature and density in low density nuclear matter produced in collisions of 47$A$ MeV $^{40}$Ar and $^{64}$Zn projectiles with $^{112}$Sn and $^{124}$Sn target nuclei. The experimentally derived values of the in medium modified binding energies are in good agreement with recent theoretical predictions based upon the implementation of Pauli blocking effects in a quantum statistical approach.

  19. Photoelectron Spectroscopy and Theoretical Studies of Anion-pi Interactions: Binding Strength and Anion Specificity

    SciTech Connect (OSTI)

    Zhang, Jian; Zhou, Bin; Sun, Zhenrong; Wang, Xue B.

    2015-01-01

    Proposed in theory and confirmed to exist, anion–? interactions have been recognized as new and important non-covalent binding forces. Despite extensive theoretical studies, numerous crystal structural identifications, and a plethora of solution phase investigations, intrinsic anion–? interaction strengths that are free from complications of condensed phases’ environments, have not been directly measured in the gas phase. Herein we present a joint photoelectron spectroscopic and theoretical study on this subject, in which tetraoxacalix[2]arene[2]triazine 1, an electron-deficient and cavity self-tunable macrocyclic was used as a charge-neutral molecular host to probe its interactions with a series of anions with distinctly different shapes and charge states (spherical halides Cl?, Br?, I?, linear thiocyanate SCN?, trigonal planar nitrate NO??, pyramidic iodate IO??, and tetrahedral sulfate SO?²?). The binding energies of the resultant gaseous 1:1 complexes (1•Cl?,1•Br?, 1•I?, 1•SCN?, 1•NO??, 1•IO?? and 1•SO?²?) were directly measured experimentally, exhibiting substantial non-covalent interactions with pronounced anion specific effects. The binding strengths of Cl?, NO??, IO?? with 1 are found to be strongest among all singly charged anions, amounting to ca. 30 kcal/mol, but only about 40% of that between 1 and SO?²?. Quantum chemical calculations reveal that all anions reside in the center of the cavity of 1 with anion–? binding motif in the complexes’ optimized structures, where 1 is seen to be able to self-regulate its cavity structure to accommodate anions of different geometries and three-dimensional shapes. Electron density surface and natural bond orbital charge distribution analysis further support anion–? binding formation. The calculated binding energies of the anions and 1 nicely reproduce the experimentally estimated electron binding energy increase. This work illustrates that size-selective photoelectron spectroscopy combined with theoretical calculations represent a powerful technique to probe intrinsic anion–? interactions and has potential to provide quantitative guest-host molecular binding strengths and unravel fundamental insights in specific anion recognitions.

  20. ENHANCING MARKETING WITH ENGINEERING: OPTIMAL PRODUCT LINE DESIGN FOR HETEROGENEOUS MARKETS

    E-Print Network [OSTI]

    Papalambros, Panos

    ENHANCING MARKETING WITH ENGINEERING: OPTIMAL PRODUCT LINE DESIGN FOR HETEROGENEOUS MARKETS Jeremy of Business. #12;- 1 - ENHANCING MARKETING WITH ENGINEERING: OPTIMAL PRODUCT LINE DESIGN FOR HETEROGENEOUS MARKETS ABSTRACT Successful product line design and development often requires balancing technical