Sample records for binds dashed line

  1. thors added dashed lines to indicate the subsequent liquid phase composition with reducing relative

    E-Print Network [OSTI]

    disappear entirely through the absorption or release of aqueous phase material with an associated crysthors added dashed lines to indicate the subsequent liquid phase composition with reducing relative to change the aqueous composition. The two cases are respectively distinguished by a local minimum

  2. Dashed lines relationships

    E-Print Network [OSTI]

    2 MAG1 DUN1DIN7 GPG1 RFA2 HOR7 NAB2 TPS1 YOL155C YER079W YHR138C YFR017C YLR297W YBR053C YPR015C YIL

  3. Demonstration of Datacenter Automation Software and Hardware (DASH) at the California Franchise Tax Board

    E-Print Network [OSTI]

    Bell, Geoffrey C.

    2010-01-01T23:59:59.000Z

    of Datacenter Automation Software and Hardware (DASH) at theof Datacenter Automation Software and Hardware (DASH) at theprotocol for building automation and control networks. It is

  4. Particle trap to sheath non-binding contact for a gas-insulated transmission line having a corrugated outer conductor

    DOE Patents [OSTI]

    Fischer, William H. (Pittsburgh, PA)

    1984-04-24T23:59:59.000Z

    A non-binding particle trap to outer sheath contact for use in gas insulated transmission lines having a corrugated outer conductor. The non-binding feature of the contact according to the teachings of the invention is accomplished by having a lever arm rotatably attached to a particle trap by a pivot support axis disposed parallel to the direction of travel of the inner conductor/insulator/particle trap assembly.

  5. Dash for Bikes, Walk for Trikes | National Nuclear Security Administration

    National Nuclear Security Administration (NNSA)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742EnergyOn AprilA groupTubahq.na.gov Office of theNuclearNanotechnologiesSafeguardsQuarterlyDash

  6. The effects of temperature on thyroid hormone binding to serum proteins in sea turtles

    E-Print Network [OSTI]

    Haynes, Shane Patrick

    1990-01-01T23:59:59.000Z

    . S. , Texas AKN University Chair of Advisory Committee: Dr. Duncan S. HacKenzie The high affinity binding of thyroid hormones to plasma proteins is a critical step in their peripheral delivery. This study was undertaken to investigate whether... changes in temperature might alter thyroid hormone binding to plasma binding proteins in poikilotherms. The objectives were to determine if high affinity thyroid hormo plasma hi ding proteins e ist i the green (ghelo ia %dash, ioggerhead tata caretta...

  7. Figure 3. Socioeconomics drive biomass too. Simple regression with untrans-formed variables. Solid line represents the estimated regression line, whereas

    E-Print Network [OSTI]

    Hall, Sharon J.

    Figure 3. Socioeconomics drive biomass too. Simple regression with untrans- formed variables. Solid line represents the estimated regression line, whereas the dashed lines represent the 95% confidence metropolitan area. I hypothesized that income is the driving factor of vegetation coverage, primarily affecting

  8. Flow Test At Fenton Hill HDR Geothermal Area (Dash, Et Al., 1983) | Open

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directedAnnualPropertyd8c-a9ae-f8521cbb8489Information Hydro Inc IosilEnergy Information Area (Dash,

  9. Welcome to our program today--Hypertension: It's Prevention and Control with the DASH Eating Plan. May is National Hypertension month, but this is

    E-Print Network [OSTI]

    Welcome to our program today--Hypertension: It's Prevention and Control with the DASH Eating Plan. May is National Hypertension month, but this is information for your use throughout the year. 1 2011Hypertension: Its Prevention and Control with the DASH Eating Plan #12;Welcome to our program today--Hypertension

  10. Broadband emission in InAs/InGaAlAs quantum-dash-in-well laser Boon S. Ooi1, a

    E-Print Network [OSTI]

    -broadband sources are generated using nonlinear-optical transformations of ultra-short laser pulses and photonicBroadband emission in InAs/InGaAlAs quantum-dash-in-well laser Boon S. Ooi1, a , Hery S. Djie1: Quantum dash, Quantum dot, Broadband emission, Semiconductor Laser. Abstract. We report on the development

  11. Greater accordance with the DASH dietary pattern is associated with lower diet-related greenhouse gas production but higher dietary costs in the United Kingdom

    E-Print Network [OSTI]

    Monsivais, Pablo; Scarborough, Peter; Lloyd, Tina; Mizdrak, Anja; Luben, Robert; Mulligan, Angela A.; Wareham, Nicholas J.; Woodcock, James

    2015-04-30T23:59:59.000Z

    of the DASH score. The FFQ data were processed by using the FFQ EPIC Tool for Analysis (17), software based on the earlier analysis system (16), to estimate average daily nutrient and energy intakes. Dietary data were deemed implausible when energy estimates... : climate, diet quality, food prices, prevention, public health INTRODUCTION Diets that are consistent with the Dietary Approaches to Stop Hypertension (DASH)5 are associated with reduced cardiometabolic risk and better health outcomes. The design...

  12. A Dash of Salt

    E-Print Network [OSTI]

    Supercinski, Danielle

    2006-01-01T23:59:59.000Z

    and Agricultural Engineering; Vanessa Kelly, Jeremy Rice, Nick Russo III and Kati Stoddard, Water Management and Hydrologic Sciences; Trevor Knight, Department of Wildlife and Fisheries Sciences; Meredith Langille, Oke Nwaneshiudu and Sucheta Parkhi...

  13. DASH | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directedAnnual Siteof EnergyInnovationinConcentratingEnergyCoosaPageEnergy Information New Energy

  14. Branching and Bounding Improvements for Global Optimization ...

    E-Print Network [OSTI]

    Coralia Cartis, Jaroslav M. Fowkes, Nicholas I. M. Gould

    2014-04-29T23:59:59.000Z

    to use generalisations of the power method using multiple starting points (Kolda ..... first order (dotted, solid and dash-dotted lines) and second order (dashed ...

  15. Melanin-binding radiopharmaceuticals

    SciTech Connect (OSTI)

    Packer, S; Fairchild, R G; Watts, K P; Greenberg, D; Hannon, S J

    1980-01-01T23:59:59.000Z

    The scope of this paper is limited to an analysis of the factors that are important to the relationship of radiopharmaceuticals to melanin. While the authors do not attempt to deal with differences between melanin-binding vs. melanoma-binding, a notable variance is assumed. (PSB)

  16. Interseismic strain accumulation and the earthquake potential on the southern San Andreas fault system

    E-Print Network [OSTI]

    Fialko, Y

    2006-01-01T23:59:59.000Z

    driving the interseismic deformation at the brittle-ductile transition are denoted by dashed green lines

  17. Cellulose binding domain proteins

    DOE Patents [OSTI]

    Shoseyov, Oded (Karmey Yosef, IL); Shpiegl, Itai (Rehovot, IL); Goldstein, Marc (Davis, CA); Doi, Roy (Davis, CA)

    1998-01-01T23:59:59.000Z

    A cellulose binding domain (CBD) having a high affinity for crystalline cellulose and chitin is disclosed, along with methods for the molecular cloning and recombinant production thereof. Fusion products comprising the CBD and a second protein are likewise described. A wide range of applications are contemplated for both the CBD and the fusion products, including drug delivery, affinity separations, and diagnostic techniques.

  18. Cellulose binding domain proteins

    DOE Patents [OSTI]

    Shoseyov, O.; Shpiegl, I.; Goldstein, M.; Doi, R.

    1998-11-17T23:59:59.000Z

    A cellulose binding domain (CBD) having a high affinity for crystalline cellulose and chitin is disclosed, along with methods for the molecular cloning and recombinant production. Fusion products comprising the CBD and a second protein are likewise described. A wide range of applications are contemplated for both the CBD and the fusion products, including drug delivery, affinity separations, and diagnostic techniques. 16 figs.

  19. Changes in misonidazole binding with hypoxic fraction in mouse tumors

    SciTech Connect (OSTI)

    Hirst, D.G.; Hazlehurst, J.L.; Brown, J.M.

    1985-07-01T23:59:59.000Z

    Binding of misonidazole (MISO) or a derivative to hypoxic cells in tumors has been proposed as a method for identifying tumors, and measuring their level of hypoxia. The author has recently shown that the hypoxic fraction of tumor cells can be altered over a wide range in vivo by acutely changing the hematocrit of the host animal by transfusion. The present study is aimed to investigate the changes in binding by /sup 14/C MISO that accompanied this procedure. Tumor bearing mice were injected with /sup 14/C MISO, irradiated with a single dose of X rays (20 Gy) and their tumor excised and bisected. One half of each tumor was used to determine cell survival in vitro, the other was used for /sup 14/C scintillation counting. As previously described, tumor cell survival was dramatically increased in acutely anemic mice and this was accompanied by an increase in /sup 14/C MISO binding to the tumors. The relationship between clonogenic cell survival and binding was found to be linear on a log-log plot for each of the tumor lines studied, but the slopes of the lines were different in different tumor lines and generally steeper than the value of 1.0 expected for a 1:1 correspondence between cells binding radioactivity and radiobiological resistance.

  20. Plains and Eastern Clean Line Transmission Line: Comment from...

    Office of Environmental Management (EM)

    from Block Plains and Eastern Clean Line: Arkansas and Oklahoma Plains and Eastern Clean Line Transmission Line: Comment from Block Plains and Eastern Clean Line: Arkansas and...

  1. Mad Dash Inc | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to: navigation, searchOfRose Bend < MHKconverter <WAG BuoyYOG

  2. Synthetic heparin-binding growth factor analogs

    DOE Patents [OSTI]

    Pena, Louis A.; Zamora, Paul; Lin, Xinhua; Glass, John D.

    2007-01-23T23:59:59.000Z

    The invention provides synthetic heparin-binding growth factor analogs having at least one peptide chain that binds a heparin-binding growth factor receptor, covalently bound to a hydrophobic linker, which is in turn covalently bound to a non-signaling peptide that includes a heparin-binding domain. The synthetic heparin-binding growth factor analogs are useful as soluble biologics or as surface coatings for medical devices.

  3. Bounds to binding energies from the concavity of thermodynamical functions

    E-Print Network [OSTI]

    B. K. Jennings; B. R. Barrett; B. G. Giraud

    2007-08-22T23:59:59.000Z

    Sequences of experimental ground-state energies are mapped onto concave patterns cured from convexities due to pairing and/or shell effects. The same patterns, completed by a list of excitation energies, can be used to give numerical estimates of the grand potential $\\Omega(\\beta,\\mu)$ for a mixture of nuclei at low or moderate temperatures $T=\\beta^{-1}$ and at many chemical potentials $\\mu.$ The average nucleon number $(\\beta,\\mu)$ then becomes a continuous variable, allowing extrapolations towards nuclear masses closer to drip lines. We study the possible concavity of several thermodynamical functions, such as the free energy and the average energy, as functions of $.$ Concavity, when present in such functions, allows trivial interpolations and extrapolations providing upper and lower bounds, respectively, to binding energies. Such bounds define an error bar for the prediction of binding energies. An extrapolation scheme for such concave functions is tested. We conclude with numerical estimates of the binding energies of a few nuclei closer to drip lines.

  4. Cellulose binding domain fusion proteins

    DOE Patents [OSTI]

    Shoseyov, O.; Yosef, K.; Shpiegl, I.; Goldstein, M.A.; Doi, R.H.

    1998-02-17T23:59:59.000Z

    A cellulose binding domain (CBD) having a high affinity for crystalline cellulose and chitin is disclosed, along with methods for the molecular cloning and recombinant production. Fusion products comprising the CBD and a second protein are likewise described. A wide range of applications are contemplated for both the CBD and the fusion products, including drug delivery, affinity separations, and diagnostic techniques. 16 figs.

  5. Cellulose binding domain fusion proteins

    DOE Patents [OSTI]

    Shoseyov, Oded (Karmey Yosef, IL); Shpiegl, Itai (Rehovot, IL); Goldstein, Marc A. (Davis, CA); Doi, Roy H. (Davis, CA)

    1998-01-01T23:59:59.000Z

    A cellulose binding domain (CBD) having a high affinity for crystalline cellulose and chitin is disclosed, along with methods for the molecular cloning and recombinant production thereof. Fusion products comprising the CBD and a second protein are likewise described. A wide range of applications are contemplated for both the CBD and the fusion products, including drug delivery, affinity separations, and diagnostic techniques.

  6. Erythropoietin binding protein from mammalian serum

    DOE Patents [OSTI]

    Clemons, Gisela K. (Berkeley, CA)

    1997-01-01T23:59:59.000Z

    Purified mammalian erythropoietin binding-protein is disclosed, and its isolation, identification, characterization, purification, and immunoassay are described. The erythropoietin binding protein can be used for regulation of erythropoiesis by regulating levels and half-life of erythropoietin. A diagnostic kit for determination of level of erythropoietin binding protein is also described.

  7. Erythropoietin binding protein from mammalian serum

    DOE Patents [OSTI]

    Clemons, G.K.

    1997-04-29T23:59:59.000Z

    Purified mammalian erythropoietin binding-protein is disclosed, and its isolation, identification, characterization, purification, and immunoassay are described. The erythropoietin binding protein can be used for regulation of erythropoiesis by regulating levels and half-life of erythropoietin. A diagnostic kit for determination of level of erythropoietin binding protein is also described. 11 figs.

  8. Series Transmission Line Transformer

    DOE Patents [OSTI]

    Buckles, Robert A. (Livermore, CA); Booth, Rex (Livermore, CA); Yen, Boris T. (El Cerrito, CA)

    2004-06-29T23:59:59.000Z

    A series transmission line transformer is set forth which includes two or more of impedance matched sets of at least two transmissions lines such as shielded cables, connected in parallel at one end ans series at the other in a cascading fashion. The cables are wound about a magnetic core. The series transmission line transformer (STLT) which can provide for higher impedance ratios and bandwidths, which is scalable, and which is of simpler design and construction.

  9. Electric Transmission Lines (Nebraska)

    Broader source: Energy.gov [DOE]

    The Public Service Commission has jurisdiction over all electricity transmission lines crossing over or under railroad tracks at public highway crossings. This section contains general regulations...

  10. The Insulator Binding Protein CTCF Positions 20 Nucleosomes around Its Binding Sites across the Human

    E-Print Network [OSTI]

    Weng, Zhiping

    The Insulator Binding Protein CTCF Positions 20 Nucleosomes around Its Binding Sites across occupied by the insulator binding protein CTCF across the human genome. These nucleosomes are highly of CTCF function. Citation: Fu Y, Sinha M, Peterson CL, Weng Z (2008) The Insulator Binding Protein CTCF

  11. A Rare Earth-DOTA-Binding Antibody: Probe Properties and Binding Affinity across the Lanthanide Series

    E-Print Network [OSTI]

    Fisher, Andrew J.

    1) binds transition metals and rare earths with extreme stability under physiological conditionsA Rare Earth-DOTA-Binding Antibody: Probe Properties and Binding Affinity across the Lanthanide affinity and exquisite specificity.1 An antibody that binds rare earth complexes selectively could be used

  12. Synthetic heparin-binding factor analogs

    DOE Patents [OSTI]

    Pena, Louis A. (Poquott, NY); Zamora, Paul O. (Gaithersburg, MD); Lin, Xinhua (Plainview, NY); Glass, John D. (Shoreham, NY)

    2010-04-20T23:59:59.000Z

    The invention provides synthetic heparin-binding growth factor analogs having at least one peptide chain, and preferably two peptide chains branched from a dipeptide branch moiety composed of two trifunctional amino acid residues, which peptide chain or chains bind a heparin-binding growth factor receptor and are covalently bound to a non-signaling peptide that includes a heparin-binding domain, preferably by a linker, which may be a hydrophobic linker. The synthetic heparin-binding growth factor analogs are useful as pharmaceutical agents, soluble biologics or as surface coatings for medical devices.

  13. Brief Communications cAMP Response Element-Binding Protein 1 Feedback Loop

    E-Print Network [OSTI]

    Byrne, John H.

    is creb1 (Mohamed et al., 2005), the Aplysia homolog of mammalian creb (Bartsch et al., 1998, 1998). CREB1-mediated gene expression is required for induction of LTF (Dash et al., 1990; Bartsch et et al., 1994; O'Leary et al., 1995). Previous studies (Bartsch et al., 1998) showed that levels

  14. Transmission Line Security Monitor

    ScienceCinema (OSTI)

    None

    2013-05-28T23:59:59.000Z

    The Transmission Line Security Monitor is a multi-sensor monitor that mounts directly on high-voltage transmission lines to detect, characterize and communicate terrorist activity, human tampering and threatening conditions around support towers. For more information about INL's critical infrastructure protection research, visit http://www.facebook.com/idahonationallaboratory.

  15. Transmission Line Security Monitor

    SciTech Connect (OSTI)

    None

    2011-01-01T23:59:59.000Z

    The Transmission Line Security Monitor is a multi-sensor monitor that mounts directly on high-voltage transmission lines to detect, characterize and communicate terrorist activity, human tampering and threatening conditions around support towers. For more information about INL's critical infrastructure protection research, visit http://www.facebook.com/idahonationallaboratory.

  16. Intrinsic AGN Absorption Lines

    E-Print Network [OSTI]

    Fred Hamann

    2000-06-12T23:59:59.000Z

    Strong absorption lines are common in rest-frame UV spectra of AGNs due to a variety of resonant transitions, for example the HI Lyman series lines (most notably Ly-alpha 1216) and high-ionization doublets like CIV 1549,1551. The lines are called ``intrinsic'' if the absorbing gas is physically related to the AGN, e.g. if the absorber resides broadly within the radius of the AGN's surrounding ``host'' galaxy. Intrinsic absorption lines are thus valuable probes of the kinematics, physical conditions and elemental abundances in the gas near AGNs. Studies of intrinsic absorbers have historically emphasized the broad absorption lines (BALs) in quasars. Today we recognize a wider variety of intrinsic lines in a wider range of objects. For example, we now know that Seyfert 1 galaxies (the less luminous cousins of quasars) have intrinsic absorption. We also realize that intrinsic lines can form in a range of AGN environments --- from the dynamic inner regions like the BALs, to the more quiescent outer host galaxies >10 kpc away. This article provides a brief introduction to current observational and theoretical work on intrinsic AGN absorbers.

  17. Coherent soliton communication lines

    SciTech Connect (OSTI)

    Yushko, O. V., E-mail: olesya.yushko@gmail.com; Redyuk, A. A.; Fedoruk, M. P.; Turitsyn, S. K. [Novosibirsk State University (Russian Federation)

    2014-11-15T23:59:59.000Z

    The data transmission in coherent fiber-optical communication lines using solitons with a variable phase is studied. It is shown that nonlinear coherent structures (solitons) can be applied for effective signal transmission over a long distance using amplitude and optical-phase keying of information. The optimum ratio of the pulse width to the bit slot at which the spectral efficiency (transmitted bits per second and hertz) is maximal is determined. It is shown that soliton fiber-optical communication lines can ensure data transmission at a higher spectral efficiency as compared to traditional communication lines and at a high signal-to-noise ratio.

  18. Holes in Spectral Lines

    E-Print Network [OSTI]

    Fontana, Peter R.; Srivastava, Rajendra P.

    1973-06-01T23:59:59.000Z

    The decay of an atom in the presence of a static perturbation is investigated. The perturbation couples a decaying state with a nondecaying state. A "hole" appears in the emission line at a frequency equal to the frequency difference between...

  19. Line Extension Alternatives

    Broader source: Energy.gov [DOE]

    The Public Service Board (PSB) of Vermont developed rules regarding utility line extension requests. While the majority of the rules focus on the procedure followed (and associated fees) for the...

  20. Electric Transmission Lines (Iowa)

    Broader source: Energy.gov [DOE]

    Electric transmission lines capable of operating at 69 kV or greater cannot be constructed along, across, or over any public highways or grounds outside of cities without a franchise from the...

  1. Power line detection system

    DOE Patents [OSTI]

    Latorre, V.R.; Watwood, D.B.

    1994-09-27T23:59:59.000Z

    A short-range, radio frequency (RF) transmitting-receiving system that provides both visual and audio warnings to the pilot of a helicopter or light aircraft of an up-coming power transmission line complex. Small, milliwatt-level narrowband transmitters, powered by the transmission line itself, are installed on top of selected transmission line support towers or within existing warning balls, and provide a continuous RF signal to approaching aircraft. The on-board receiver can be either a separate unit or a portion of the existing avionics, and can also share an existing antenna with another airborne system. Upon receipt of a warning signal, the receiver will trigger a visual and an audio alarm to alert the pilot to the potential power line hazard. 4 figs.

  2. Power line detection system

    DOE Patents [OSTI]

    Latorre, Victor R. (Tracy, CA); Watwood, Donald B. (Tracy, CA)

    1994-01-01T23:59:59.000Z

    A short-range, radio frequency (RF) transmitting-receiving system that provides both visual and audio warnings to the pilot of a helicopter or light aircraft of an up-coming power transmission line complex. Small, milliwatt-level narrowband transmitters, powered by the transmission line itself, are installed on top of selected transmission line support towers or within existing warning balls, and provide a continuous RF signal to approaching aircraft. The on-board receiver can be either a separate unit or a portion of the existing avionics, and can also share an existing antenna with another airborne system. Upon receipt of a warning signal, the receiver will trigger a visual and an audio alarm to alert the pilot to the potential power line hazard.

  3. Omega Transmission Lines

    E-Print Network [OSTI]

    Vehmas, Joni

    2013-01-01T23:59:59.000Z

    In this paper, we show how bi-anisotropic media with omega-type response can be realized using periodically loaded transmission lines. General conditions for the needed unit cell circuit block are derived. Also, an implementation is shown and analyzed.

  4. Command Line Tools Cloud Computing

    E-Print Network [OSTI]

    Ferrara, Katherine W.

    Command Line Tools Cloud Computing #12;Everybody (or nearly everybody) loves GUI. AWS Command Line of advanced features. After surviving the cloud computing class till now, Your are almost a command line guru! You need AWS command line tools, ec2-api-tools, to maximize the power of AWS cloud computing. Plugging

  5. Printed circuit dispersive transmission line

    DOE Patents [OSTI]

    Ikezi, H.; Lin-Liu, Y.R.; DeGrassie, J.S.

    1991-08-27T23:59:59.000Z

    A printed circuit dispersive transmission line structure is disclosed comprising an insulator, a ground plane formed on one surface of the insulator, a first transmission line formed on a second surface of the insulator, and a second transmission line also formed on the second surface of the insulator and of longer length than the first transmission line and periodically intersecting the first transmission line. In a preferred embodiment, the transmission line structure exhibits highly dispersive characteristics by designing the length of one of the transmission line between two adjacent periodic intersections to be longer than the other. 5 figures.

  6. Formation depths of Fraunhofer lines

    E-Print Network [OSTI]

    Gurtovenko, E A

    2015-01-01T23:59:59.000Z

    We have summed up our investigations performed in 1970--1993. The main task of this paper is clearly to show processes of formation of spectral lines as well as their distinction by validity and by location. For 503 photospheric lines of various chemical elements in the wavelength range 300--1000 nm we list in Table the average formation depths of the line depression and the line emission for the line centre and on the half-width of the line, the average formation depths of the continuum emission as well as the effective widths of the layer of the line depression formation. Dependence of average depths of line depression formation on excitation potential, equivalent widths, and central line depth are demonstrated by iron lines.

  7. Crystallographic controls on uranyl binding at the quartz/water...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    controls on uranyl binding at the quartzwater interface. Abstract: Molecular dynamics methods were used to simulate UO2(OH)20 binding to pairs of oxo sites on three...

  8. Single-Molecule Dynamics Reveals Cooperative Binding-Folding...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    biased towards the native binding structure (Go model). With our model, the underlying free energy landscape of the binding can be explored. Two distinct conformational states...

  9. Atomic structure of nitrate-binding protein crucial for photosynthetic...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    structure of nitrate-binding protein crucial for photosynthetic productivity. Atomic structure of nitrate-binding protein crucial for photosynthetic productivity. Abstract:...

  10. affinity binding studies: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    for Ligand Binding Affinity Prediction Chemistry Websites Summary: of the binding free energy between a ligand and a protein is an important component in the virtual...

  11. Sequestering Uranium from Seawater: Binding Strength and Modes...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Sequestering Uranium from Seawater: Binding Strength and Modes of Uranyl Complexes with Glutarimidedioxime Sequestering Uranium from Seawater: Binding Strength and Modes of Uranyl...

  12. Drill string transmission line

    DOE Patents [OSTI]

    Hall, David R. (Provo, UT); Hall, Jr., H. Tracy (Provo, UT); Pixton, David S. (Lehi, UT); Bradford, Kline (Orem, UT); Fox, Joe (Spanish Fork, UT)

    2006-03-28T23:59:59.000Z

    A transmission line assembly for transmitting information along a downhole tool comprising a pin end, a box end, and a central bore traveling between the pin end and the box end, is disclosed in one embodiment of the invention as including a protective conduit. A transmission line is routed through the protective conduit. The protective conduit is routed through the central bore and the ends of the protective conduit are routed through channels formed in the pin end and box end of the downhole tool. The protective conduit is elastically forced into a spiral or other non-linear path along the interior surface of the central bore by compressing the protective conduit to a length within the downhole tool shorter than the protective conduit.

  13. Transmission line: design manual

    SciTech Connect (OSTI)

    Farr, H.H.

    1980-01-01T23:59:59.000Z

    The purpose of this manual is to outline the various requirements for, and the procedures to be followed in the design of power transmission lines by the Bureau of Reclamation, US Department of the Interior. Numerous design studies, which have been made on specific aspects of transmission line design, are included with explanations of their applications. Information is presented concerning such aspects as selection of type of construction, conductor sags and tensions, insulation, lightning protection, clearance patterns, galloping conductors, structure limitation and guying charts, and structure spotting. Structure design examples are limited to wood-pole construction. Interpretations of the National Electrical Safety Code and other codes are made as required. Some of the example problems were developed when the sixth edition of NESC was current, and are so noted; however, most examples use the 1977 edition of NESC.

  14. Hardware device binding and mutual authentication

    DOE Patents [OSTI]

    Hamlet, Jason R; Pierson, Lyndon G

    2014-03-04T23:59:59.000Z

    Detection and deterrence of device tampering and subversion by substitution may be achieved by including a cryptographic unit within a computing device for binding multiple hardware devices and mutually authenticating the devices. The cryptographic unit includes a physically unclonable function ("PUF") circuit disposed in or on the hardware device, which generates a binding PUF value. The cryptographic unit uses the binding PUF value during an enrollment phase and subsequent authentication phases. During a subsequent authentication phase, the cryptographic unit uses the binding PUF values of the multiple hardware devices to generate a challenge to send to the other device, and to verify a challenge received from the other device to mutually authenticate the hardware devices.

  15. RNA binding protein and binding site useful for expression of recombinant molecules

    DOE Patents [OSTI]

    Mayfield, Stephen P.

    2006-10-17T23:59:59.000Z

    The present invention relates to a gene expression system in eukaryotic and prokaryotic cells, preferably plant cells and intact plants. In particular, the invention relates to an expression system having a RB47 binding site upstream of a translation initiation site for regulation of translation mediated by binding of RB47 protein, a member of the poly(A) binding protein family. Regulation is further effected by RB60, a protein disulfide isomerase. The expression system is capable of functioning in the nuclear/cytoplasm of cells and in the chloroplast of plants. Translation regulation of a desired molecule is enhanced approximately 100 fold over that obtained without RB47 binding site activation.

  16. RNA binding protein and binding site useful for expression of recombinant molecules

    DOE Patents [OSTI]

    Mayfield, Stephen (Cardiff, CA)

    2000-01-01T23:59:59.000Z

    The present invention relates to a gene expression system in eukaryotic and prokaryotic cells, preferably plant cells and intact plants. In particular, the invention relates to an expression system having a RB47 binding site upstream of a translation initiation site for regulation of translation mediated by binding of RB47 protein, a member of the poly(A) binding protein family. Regulation is further effected by RB60, a protein disulfide isomerase. The expression system is capable of functioning in the nuclear/cytoplasm of cells and in the chloroplast of plants. Translation regulation of a desired molecule is enhanced approximately 100 fold over that obtained without RB47 binding site activation.

  17. Utility Lines and Facilities (Montana)

    Broader source: Energy.gov [DOE]

    These regulations apply to the construction of utility and power lines and facilities. They address the use of public right-of-ways for such construction, underground power lines, and construction...

  18. Maersk Line Equipment guide

    National Nuclear Security Administration (NNSA)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742EnergyOn AprilA Approved:AdministrationAnalysisDarby/%2AOU1a ComplexMaersk Line Equipment

  19. Polypyrimidine Track-binding Protein Binding Downstream of Caspase-2 Alternative Exon 9 Represses Its Inclusion*

    E-Print Network [OSTI]

    Wu, Jane Y.

    Polypyrimidine Track-binding Protein Binding Downstream of Caspase-2 Alternative Exon 9 Represses (In100) located in the intron downstream of alternative exon 9. The upstream portion of this element downstream from the decoy 3 acceptor site. This downstream domain harbors several polypyrimidine track

  20. Interactions between ultraviolet light and interleukin-1 on MSH binding in both mouse melanoma and human squamous carcinoma cells

    SciTech Connect (OSTI)

    Birchall, N.; Orlow, S.J.; Kupper, T.; Pawelek, J. (Univ. of Auckland, (New Zealand))

    1991-03-29T23:59:59.000Z

    Interactions between beta-melanotropin (MSH), interleukin 1-a (IL-1), and ultraviolet light (UV) were examined in Cloudman S91 mouse melanoma and RHEK human squamous carcinoma cell lines. The following points were established: (1) both cell lines produced IL-1 and their production was stimulated by exposure of the cells to UV; (2) both cell lines possessed high affinity binding sites for MSH, and their ability to bind MSH was modulated by IL-1; (3) IL-1 exhibited both stimulatory and inhibitory effects on MSH binding to Cloudman cells; and (4) the stimulatory effect of IL-1 on MSH binding to melanoma cells was reflected in enhanced cellular responsiveness to MSH regarding tyrosinase activity (E.C. 1.14.18.1) and melanin content. The findings raise the possibility that interactions between keratinocytes and melanocytes may be regulated by IL-1 and MSH, and suggest a possible mechanism for stimulation of cutaneous melanogenesis by solar radiation: enhancement of MSH receptor activity by induction of IL-1.

  1. The Declining Use of Race in College Admissions Decisions

    E-Print Network [OSTI]

    Grodsky, Eric; Kalogrides, Demetra

    2007-01-01T23:59:59.000Z

    in affirmative action than comparable private institutionsaffirmative action is evident among both public and privateaction increases for both public institutions (dashed line) and private

  2. Localized states of a semi-infinite zigzag graphene sheet with impurity lines

    SciTech Connect (OSTI)

    Cunha, A. M. C., E-mail: amagno@fisica.ufc.br [Departamento de Física, Universidade Federal do Ceará, Caixa Postal 6030, Campus do Pici, 60455-760 Fortaleza, Ceara (Brazil); Ahmed, Maher Z. [Department of Physics and Astronomy, University of Western Ontario, London, Ontario N6A 3K7, Canada and Physics Department, Faculty of Science, Ain Shams University, Abbsai, Cairo (Egypt); Cottam, M. G., E-mail: cottam@uwo.ca [Department of Physics and Astronomy, University of Western Ontario, London, Ontario N6A 3K7 (Canada); Filho, R. N. Costa, E-mail: rai@fisica.ufc.br [Department of Physics and Astronomy, University of Western Ontario, London, Ontario N6A 3K7, Canada and Departamento de Física, Universidade Federal do Ceará, Caixa Postal 6030, Campus do Pici, 60455-760 Fortaleza, Ceara (Brazil)

    2014-07-07T23:59:59.000Z

    The localized states of a semi-infinite zigzag graphene sheet are studied using a tight-binding model that allows for the inclusion of either one or two lines of impurities. These impurity lines of atoms are placed in rows labeled as n (n?=?1, 2, 3, …), where n?=?1 is the free edge. The localized defect modes associated with these impurities are studied analytically and numerically within a tridiagonal matrix formalism. For one impurity line, the modes are analyzed according to the position of that line on the sheet, whereas the modes for two impurities are studied also according to their separation and their positions relative to the edge. When an impurity line is located at the edge (n?=?1), it is found that the edge states are modified. When the impurities are positioned away from an edge (n?>?1), additional localized modes are found to occur that may be relatively flat in their dispersion.

  3. Nucleic acids encoding a cellulose binding domain

    DOE Patents [OSTI]

    Shoseyov, Oded (Karmey Yosef, IL); Shpiegl, Itai (Rehovot, IL); Goldstein, Marc A. (Davis, CA); Doi, Roy H. (Davis, CA)

    1996-01-01T23:59:59.000Z

    A cellulose binding domain (CBD) having a high affinity for crystalline cellulose and chitin is disclosed, along with methods for the molecular cloning and recombinant production thereof. Fusion products comprising the CBD and a second protein are likewise described. A wide range of applications are contemplated for both the CBD and the fusion products, including drug delivery, affinity separations, and diagnostic techniques.

  4. Binding Energies in Nonrelativistic Field Theories

    E-Print Network [OSTI]

    Andreas S. Kronfeld

    1996-08-26T23:59:59.000Z

    Relativistic corrections communicate the binding energy of a bound state to its kinetic mass. This mechanism is reviewed and used to explain anomalous results of Collins, Edwards, Heller, and Sloan (hep-lat/9512026), which compared rest and kinetic masses of heavy-light mesons and quarkonia.

  5. Nucleic acids encoding a cellulose binding domain

    DOE Patents [OSTI]

    Shoseyov, O.; Shpiegl, I.; Goldstein, M.A.; Doi, R.H.

    1996-03-05T23:59:59.000Z

    A cellulose binding domain (CBD) having a high affinity for crystalline cellulose and chitin is disclosed, along with methods for the molecular cloning and recombinant production. Fusion products comprising the CBD and a second protein are likewise described. A wide range of applications are contemplated for both the CBD and the fusion products, including drug delivery, affinity separations, and diagnostic techniques. 15 figs.

  6. Names and Binding in Type Theory 

    E-Print Network [OSTI]

    Schöpp, Ulrich

    Names and name-binding are useful concepts in the theory and practice of formal systems. In this thesis we study them in the context of dependent type theory. We propose a novel dependent type theory with primitives for the explicit handling...

  7. Bipolar pulse forming line

    DOE Patents [OSTI]

    Rhodes, Mark A. (Pleasanton, CA)

    2008-10-21T23:59:59.000Z

    A bipolar pulse forming transmission line module for linear induction accelerators having first, second, third, fourth, and fifth planar conductors which form an interleaved stack with dielectric layers between the conductors. Each conductor has a first end, and a second end adjacent an acceleration axis. The first and second planar conductors are connected to each other at the second ends, the fourth and fifth planar conductors are connected to each other at the second ends, and the first and fifth planar conductors are connected to each other at the first ends via a shorting plate adjacent the first ends. The third planar conductor is electrically connectable to a high voltage source, and an internal switch functions to short a high voltage from the first end of the third planar conductor to the first end of the fourth planar conductor to produce a bipolar pulse at the acceleration axis with a zero net time integral. Improved access to the switch is enabled by an aperture through the shorting plate and the proximity of the aperture to the switch.

  8. EC Transmission Line Materials

    SciTech Connect (OSTI)

    Bigelow, Tim S [ORNL

    2012-05-01T23:59:59.000Z

    The purpose of this document is to identify materials acceptable for use in the US ITER Project Office (USIPO)-supplied components for the ITER Electron cyclotron Heating and Current Drive (ECH&CD) transmission lines (TL), PBS-52. The source of material property information for design analysis shall be either the applicable structural code or the ITER Material Properties Handbook. In the case of conflict, the ITER Material Properties Handbook shall take precedence. Materials selection, and use, shall follow the guidelines established in the Materials Assessment Report (MAR). Materials exposed to vacuum shall conform to the ITER Vacuum Handbook. [Ref. 2] Commercial materials shall conform to the applicable standard (e.g., ASTM, JIS, DIN) for the definition of their grade, physical, chemical and electrical properties and related testing. All materials for which a suitable certification from the supplier is not available shall be tested to determine the relevant properties, as part of the procurement. A complete traceability of all the materials including welding materials shall be provided. Halogenated materials (example: insulating materials) shall be forbidden in areas served by the detritiation systems. Exceptions must be approved by the Tritium System and Safety Section Responsible Officers.

  9. Structural basis of CX-4945 binding to human protein kinase CK2

    SciTech Connect (OSTI)

    Ferguson, Andrew D.; Sheth, Payal R.; Basso, Andrea D.; Paliwal, Sunil; Gray, Kimberly; Fischmann, Thierry O.; Le, Hung V. (Merck)

    2012-02-07T23:59:59.000Z

    Protein kinase CK2 (CK2), a constitutively active serine/threonine kinase, is involved in a variety of roles essential to the maintenance of cellular homeostasis. Elevated levels of CK2 expression results in the dysregulation of key signaling pathways that regulate transcription, and has been implicated in cancer. The adenosine-5'-triphosphate-competitive inhibitor CX-4945 has been reported to show broad spectrum anti-proliferative activity in multiple cancer cell lines. Although the enzymatic IC{sub 50} of CX-4945 has been reported, the thermodynamics and structural basis of binding to CK2{alpha} remained elusive. Presented here are the crystal structures of human CK2{alpha} in complex with CX-4945 and adenylyl phosphoramidate at 2.7 and 1.3 {angstrom}, respectively. Biophysical analysis of CX-4945 binding is also described. This data provides the structural rationale for the design of more potent inhibitors against this emerging cancer target.

  10. User Interfaces 1 Command Line Interfaces

    E-Print Network [OSTI]

    Verschelde, Jan

    User Interfaces 1 Command Line Interfaces getting arguments of the command line a command line 2013 1 / 39 #12;User Interfaces 1 Command Line Interfaces getting arguments of the command line a command line interface to store points fitting points with polyfit of numpy 2 Encapsulation by Object

  11. U-101: Mozilla Firefox / Thunderbird / SeaMonkey XBL Binding...

    Broader source: Energy.gov (indexed) [DOE]

    101: Mozilla Firefox Thunderbird SeaMonkey XBL Binding Use-After-Free Vulnerability U-101: Mozilla Firefox Thunderbird SeaMonkey XBL Binding Use-After-Free Vulnerability...

  12. acid binding proteins: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The adipocyte fatty acid-binding protein aP2 regulates systemic glucose (more) Shum, Bennett Oh Vic 2007-01-01 2 Fatty acid-binding protein in bovine skeletal muscle Texas A&M...

  13. acid dopac binds: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The adipocyte fatty acid-binding protein aP2 regulates systemic glucose (more) Shum, Bennett Oh Vic 2007-01-01 2 Fatty acid-binding protein in bovine skeletal muscle Texas A&M...

  14. Investigation of metal binding properties in the hairpin ribozyme

    E-Print Network [OSTI]

    Kirchner, Alyson Jane

    2002-01-01T23:59:59.000Z

    independently folding domains, labeled loop A and loop B. Divalent metal ions are thought to bind in specific sites within the structure and promote ribozyme folding, substrate binding, and cleavage. The goals of the present studies are to further clarify...

  15. Study of fragmentation using clusterization algorithm with realistic binding energies

    E-Print Network [OSTI]

    Yogesh K. Vermani; Jatinder K. Dhawan; Supriya Goyal; Rajeev K. Puri; J. Aichelin

    2009-12-28T23:59:59.000Z

    We here study fragmentation using \\emph{simulated annealing clusterization algorithm} (SACA) with binding energy at a microscopic level. In an earlier version, a constant binding energy (4 MeV/nucleon) was used. We improve this binding energy criterion by calculating the binding energy of different clusters using modified Bethe-Weizs\\"{a}cker mass (BWM) formula. We also compare our calculations with experimental data of ALADiN group. Nearly no effect is visible of this modification.

  16. September 18, 1994 A Binding Architecture for Multimedia Networks

    E-Print Network [OSTI]

    Columbia University

    1 September 18, 1994 A Binding Architecture for Multimedia Networks Aurel A. Lazar , Shailendra K of a set of interfaces, methods and primitives. The former abstract the functionalities of multimedia for implementing binding applications. The binding architecture is embedded into a reference model for multimedia

  17. Tight Binding Hamiltonians and Quantum Turing Machines

    E-Print Network [OSTI]

    Paul Benioff

    1996-10-17T23:59:59.000Z

    This paper extends work done to date on quantum computation by associating potentials with different types of computation steps. Quantum Turing machine Hamiltonians, generalized to include potentials, correspond to sums over tight binding Hamiltonians each with a different potential distribution. Which distribution applies is determined by the initial state. An example, which enumerates the integers in succession as binary strings, is analyzed. It is seen that for some initial states the potential distributions have quasicrystalline properties and are similar to a substitution sequence.

  18. Pulse shaping with transmission lines

    DOE Patents [OSTI]

    Wilcox, Russell B. (Oakland, CA)

    1987-01-01T23:59:59.000Z

    A method and apparatus for forming shaped voltage pulses uses passive reflection from a transmission line with nonuniform impedance. The impedance of the reflecting line varies with length in accordance with the desired pulse shape. A high voltage input pulse is transmitted to the reflecting line. A reflected pulse is produced having the desired shape and is transmitted by pulse removal means to a load. Light activated photoconductive switches made of silicon can be utilized. The pulse shaper can be used to drive a Pockels cell to produce shaped optical pulses.

  19. Pulse shaping with transmission lines

    DOE Patents [OSTI]

    Wilcox, R.B.

    1985-08-15T23:59:59.000Z

    A method and apparatus for forming shaped voltage pulses uses passive reflection from a transmission line with nonuniform impedance. The impedance of the reflecting line varies with length in accordance with the desired pulse shape. A high voltage input pulse is transmitted to the reflecting line. A reflected pulse is produced having the desired shape and is transmitted by pulse removal means to a load. Light activated photoconductive switches made of silicon can be utilized. The pulse shaper can be used to drive a Pockels cell to produce shaped optical pulses.

  20. Electrical transmission line diametrical retainer

    DOE Patents [OSTI]

    Hall, David R. (Provo, UT); Hall, Jr., H. Tracy (Provo, UT); Pixton, David (Lehi, UT); Dahlgren, Scott (Provo, UT); Sneddon, Cameron (Provo, UT); Briscoe, Michael (Lehi, UT); Fox, Joe (Spanish Fork, UT)

    2004-12-14T23:59:59.000Z

    The invention is a mechanism for retaining an electrical transmission line. In one embodiment of the invention it is a system for retaining an electrical transmission line within down hole components. In accordance with one aspect of the invention, the system includes a plurality of downhole components, such as sections of pipe in a drill string. The system also includes a coaxial cable running between the first and second end of a drill pipe, the coaxial cable having a conductive tube and a conductive core within it. The invention allows the electrical transmission line to with stand the tension and compression of drill pipe during routine drilling cycles.

  1. Dual chain synthetic heparin-binding growth factor analogs

    DOE Patents [OSTI]

    Zamora, Paul O. (Gaithersburg, MD); Pena, Louis A. (Poquott, NY); Lin, Xinhua (Plainview, NY)

    2012-04-24T23:59:59.000Z

    The invention provides synthetic heparin-binding growth factor analogs having two peptide chains each branched from a branch moiety, such as trifunctional amino acid residues, the branch moieties separated by a first linker of from 3 to about 20 backbone atoms, which peptide chains bind a heparin-binding growth factor receptor and are covalently bound to a non-signaling peptide that includes a heparin-binding domain, preferably by a second linker, which may be a hydrophobic second linker. The synthetic heparin-binding growth factor analogs are useful as pharmaceutical agents, soluble biologics or as surface coatings for medical devices.

  2. Dual chain synthetic heparin-binding growth factor analogs

    DOE Patents [OSTI]

    Zamora, Paul O. (Gaithersburg, MD); Pena, Louis A. (Poquott, NY); Lin, Xinhua (Plainview, NY)

    2009-10-06T23:59:59.000Z

    The invention provides synthetic heparin-binding growth factor analogs having two peptide chains each branched from a branch moiety, such as trifunctional amino acid residues, the branch moieties separated by a first linker of from 3 to about 20 backbone atoms, which peptide chains bind a heparin-binding growth factor receptor and are covalently bound to a non-signaling peptide that includes a heparin-binding domain, preferably by a second linker, which may be a hydrophobic second linker. The synthetic heparin-binding growth factor analogs are useful as pharmaceutical agents, soluble biologics or as surface coatings for medical devices.

  3. Transmission Lines Emulating Moving Media

    E-Print Network [OSTI]

    Vehmas, Joni; Tretyakov, Sergei

    2014-01-01T23:59:59.000Z

    In this paper, we show how the electromagnetic phenomena in moving magnetodielectric media can be emulated using artificial composite structures at rest. In particular, we introduce nonreciprocal periodically loaded transmission lines which support waves obeying the same rules as plane electromagnetic waves in moving media. Because the actual physical structure is at rest, in these transmission lines there are no fundamental limitations on the velocity values, which may take values larger than the speed of light or even complex values (considering complex amplitudes in the time-harmonic regime). An example circuit of a unit cell of a "moving" transmission line is presented and analyzed both numerically and experimentally. The special case of composite right/left handed host line is also studied numerically. Besides the fundamental interest, the study is relevant for potential applications in realizing engineered materials for various transformations of electromagnetic fields.

  4. The beauty of laser lines

    E-Print Network [OSTI]

    Kim, Sue-Mie

    1993-01-01T23:59:59.000Z

    A line, the simplest way to express an artist's feelings or interpretation of an object, has its own emotions that an artist can employ for her purpose. Laser light, the most self-concentrated, self-sustained and directed, ...

  5. Authorization of Line Extension (Nebraska)

    Broader source: Energy.gov [DOE]

    Any entity permitted to establish an electric light and power plant, and/or transmission or distribution lines within a city, village, or public electric light and power district, may also extend...

  6. VISAR: Line-imaging interferometer

    SciTech Connect (OSTI)

    Hemsing, W.F.; Mathews, A.R.; Warnes, R.H.; George, M.J.; Whittemore, G.R.

    1991-01-01T23:59:59.000Z

    A line-imaging Velocity Interferometer System for Any Reflector (VISAR) was applied to measure velocity across the diameter of a metal plate explosively accelerated to 5.5 km/s. Amplified, single- frequency laser light was focused to illuminate a line on the metal surface. The line's image was focused through the interferometer to a streak camera that swept in time and recorded directly on film. During the experiment, the Doppler-shift caused motion of the interference fringes. Analysis of the digitized film record yielded a continuum of time-resolved velocity histories. Velocity gradients across the plate that first swept radially inward, then reflected outward, were clearly measured. Increased power provided by the laser amplifier greatly improved the signal-to-noise ratio compared to our previous line VISAR experiments. 5 refs., 8 figs.

  7. Analysis of interconnect microstrip lines

    E-Print Network [OSTI]

    Luong, Giam-Minh

    1992-01-01T23:59:59.000Z

    of the return loss of the slot-coupled microstrip dipole. Section C presents two simulations of the return loss on the slot- coupled rectangular patch antenna. A. Interconnect of Microstrip Lines Several circuits with the geometry of Figure 1 were fabricated.... Experimental and Theoretical Results of the Interconnect Two circuits were designed and fabricated. Each circuit consists of two substrates as shown in Figure 9. One substrate has an open microstrip line etched in one side and an aperture etched...

  8. Fin-line horn antenna

    DOE Patents [OSTI]

    Reindel, John (San Diego, CA)

    1990-01-01T23:59:59.000Z

    A fin line circuit card containing a fin line slot feeds a dipole antenna ich extends a quarterwave outside the waveguide and provides an energy beam focal point at or near the open end of the waveguide. The dipole antenna thus maintains a wide and nearly constant beamwidth, low VSWR and a circular symmetric radiation pattern for use in electronic warfare direction finding and surveillance applications.

  9. Autoradiographic localization of endothelin-1 binding sites in porcine skin

    SciTech Connect (OSTI)

    Zhao, Y.D.; Springall, D.R.; Wharton, J.; Polak, J.M. (Royal Postgraduate Medical School, London (England))

    1991-01-01T23:59:59.000Z

    Autoradiographic techniques and {sup 125}I-labeled endothelin-1 were used to study the distribution of endothelin-1 binding sites in porcine skin. Specific endothelin-1 binding sites were localized to blood vessels (capillaries, deep cutaneous vascular plexus, arteries, and arterioles), the deep dermal and connective tissue sheath of hair follicles, sebaceous and sweat glands, and arrector pili muscle. Specific binding was inhibited by endothelin-2 and endothelin-3 as well as endothelin-1. Non-specific binding was found in the epidermis and the medulla of hair follicles. No binding was found in connective tissue or fat. These vascular binding sites may represent endothelin receptors, in keeping with the known cutaneous vasoconstrictor actions of the peptide. If all binding sites are receptors, the results suggest that endothelin could also regulate the function of sweat glands and may have trophic effects in the skin.

  10. Florida Electric Transmission Line Siting Act (Florida)

    Broader source: Energy.gov [DOE]

    The Transmission Line Siting Act (TLSA) is the state’s centralized process for licensing electrical transmission lines which; (a) are 230 kV or larger; (b) cross a county line; and, (c) are 15...

  11. Tight Binding Hamiltonians and Quantum Turing Machines

    SciTech Connect (OSTI)

    Benioff, P. [Physics Division, Argonne National Laboratory, Argonne, Illinois 60439 (United States)] [Physics Division, Argonne National Laboratory, Argonne, Illinois 60439 (United States)

    1997-01-01T23:59:59.000Z

    This paper extends work done to date on quantum computation by association of potentials with different types of steps. Quantum Turing machine Hamiltonians, generalized to include potentials, correspond to sums over tight binding Hamiltonians each with a different potential distribution. Which distribution applies is determined by the initial state. An example, which enumerates the integers in succession as binary strings, is analyzed. It is seen that for some initial states, the potential distributions have quasicrystalline properties and are similar to a substitution sequence. {copyright} {ital 1997} {ital The American Physical Society}

  12. Gene encoding herbicide safener binding protein

    DOE Patents [OSTI]

    Walton, Jonathan D. (East Lansing, MI); Scott-Craig, John S. (East Lansing, MI)

    1999-01-01T23:59:59.000Z

    The cDNA encoding safener binding protein (SafBP), also referred to as SBP1, is set forth in FIG. 5 and SEQ ID No. 1. The deduced amino acid sequence is provided in FIG. 5 and SEQ ID No. 2. Methods of making and using SBP1 and SafBP to alter a plant's sensitivity to certain herbicides or a plant's responsiveness to certain safeners are also provided, as well as expression vectors, transgenic plants or other organisms transfected with said vectors and seeds from said plants.

  13. Polynucleotides encoding TRF1 binding proteins

    DOE Patents [OSTI]

    Campisi, Judith (Berkeley, CA); Kim, Sahn-Ho (Albany, CA)

    2002-01-01T23:59:59.000Z

    The present invention provides a novel telomere associated protein (Trf1-interacting nuclear protein 2 "Tin2") that hinders the binding of Trf1 to its specific telomere repeat sequence and mediates the formation of a Tin2-Trf1-telomeric DNA complex that limits telomerase access to the telomere. Also included are the corresponding nucleic acids that encode the Tin2 of the present invention, as well as mutants of Tin2. Methods of making, purifying and using Tin2 of the present invention are described. In addition, drug screening assays to identify drugs that mimic and/or complement the effect of Tin2 are presented.

  14. Conformational Variability of Organophosphorus Hydrolase upon Soman and Paraoxon Binding

    SciTech Connect (OSTI)

    Gomes, Diego Eb; Lins, Roberto D.; Pascutti, Pedro G.; Lei, Chenghong; Soares, Thereza A.

    2011-12-31T23:59:59.000Z

    The bacterial enzyme organophosphorus hydrolase (OPH) exhibits both catalytic and substrate promiscuity. It hydrolyzes bonds in a variety of phosphotriester (P-O), phosphonothioate (P-S), phosphofluoridate (P-F) and phosphonocyanate (F-CN) compounds. However, its catalytic efficiency varies markedly for different substrates, limiting the broad-range application of OPH as catalyst in the bioremediation of pesticides and chemical war agents. In the present study, pK{sub a} calculations and multiple explicit-solvent molecular dynamics (MD) simulations were performed to characterize and contrast the structural dynamics of OPH bound to two substrates hydrolyzed with very distinct catalytic efficiencies: the nerve agent soman (O-pinacolyl-methyl-phosphonofluoridate) and the pesticide paraoxon (diethyl p-nitrophenyl phosphate). pK{sub a} calculations for the substrate-bound and unbound enzyme showed a significant pK{sub a} shift from standard values ({Delta}pK{sub a} = {+-} 3 units) for residues 254His and 275Arg. MD simulations of the doubly protonated 254His revealed a dynamic hydrogen bond network connecting the catalytic residue 301Asp via 254His to 232Asp, 233Asp, 275Arg and 235Asp, and is consistent with a previously postulated proton relay mechanism to ferry protons away from the active site with substrates that do not require activation of the leaving group. Hydrogen bonds between 301Asp and 254His were persistent in the OPH-paraoxon complex but not in the OPH-soman one, suggesting a potential role for such interaction in the more efficient hydrolysis of paraoxon over soman by OPH. These results are in line with previous mutational studies of residue 254His, which led to an increase of the catalytic efficiency of OPH over soman yet decreased its efficiency for paraoxon. In addition, comparative analysis of the molecular trajectories for OPH bound to soman and paraoxon suggests that binding of the latter facilitates the conformational transition of OPH from the open to the closed substate promoting a tighter binding of paraoxon.

  15. What can emission lines tell us?

    E-Print Network [OSTI]

    G. Stasinska

    2007-04-03T23:59:59.000Z

    1 Generalities 2 Empirical diagnostics based on emission lines 3 Photoionization modelling 4 Pending questions 5 Appendix: Lists of useful lines and how to deal with them

  16. Dumbarton Express Line DB heading to Stanford Oval. AC Transit Line U on Palm Drive.

    E-Print Network [OSTI]

    Dumbarton Express Line DB heading to Stanford Oval. AC Transit Line U on Palm Drive. Photo: Karl Nielsen Photo: Dong Wang Schedules: AC Transit Line U Dumbarton Express Line DB Connections with BART Transit Line U Line U provides express service between Stanford and the Fremont BART station. Other East

  17. The effect of a cold-air outbreak on the continental shelf water of the northwestern Gulf of Mexico

    E-Print Network [OSTI]

    Parker, Charles Allen

    1968-01-01T23:59:59.000Z

    ) as observed on ALAMINOS 66-A-1, Dashed lines indicate insufficient data for objective contouring. (Figure 6A is at left. ) B. Distribution of surface salinity (parts per tnil) as observed on ALAMINGS 66-A-2. Dashed lines indicate insufficient data...

  18. A model for positron binding to polar molecules

    E-Print Network [OSTI]

    Gribakin, G F

    2015-01-01T23:59:59.000Z

    A model for positron binding to polar molecules is considered by combining the dipole potential outside the molecule with a strongly repulsive core of a given radius. Using existing experimental data on binding energies leads to unphysically small core radii for all of the molecules studied. This suggests that electron-positron correlations neglected in the simple model play a large role in determining the binding energy. We account for these by including polarization potential via perturbation theory. The improved model enables reliable predictions of binding energies to be made for a range of polar organic molecules and hydrogen cyanide, whose binding energy is known from accurate quantum chemistry calculations. The model explains the linear dependence of the binding energies on the polarizability inferred from the experimental data [Danielson et al 2009 J. Phys. B: At. Mol. Opt. Phys. 42 235203].

  19. Quasistellar Objects: Intervening Absorption Lines

    E-Print Network [OSTI]

    Jane C. Charlton; Christopher W. Churchill

    2000-05-31T23:59:59.000Z

    We briefly review, at a level appropriate for graduate students and non-specialists, the field of quasar absorption lines (QALs). Emphasis is on the intervening absorbers. We present the anatomy of a quasar spectrum due to various classes of intervening absorption systems, and a brief historical review of each absorber class (Lyman-alpha forest and Lyman limit systems, and metal-line and damped Lyman-alpha absorbers). We also provide several heuristic examples on how the physical properties of both the intergalactic medium and the gaseous environments associated with earlier epoch galaxies can be inferred from QALs. The evolution of these environments from z=4 are discussed.

  20. Microsoft Word - Defense Science Quarterly 05-08.doc

    National Nuclear Security Administration (NNSA)

    flyer plates (blue and green solid lines) as well as predicted shock response (blue and green dashed lines). Note: Red and black lines show a control experiment conducted with a...

  1. DOE/ID-Number

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    data following 4 hour exposure to pH 3.2 H 2 SO 4 . Black line - from Cartledge and Smith (1955 ). Red line - from Cobble et al. (1953). Dashed line marked (a) represents the...

  2. On the depinning of a driven drop on a heterogeneous substrate

    E-Print Network [OSTI]

    Thiele, Uwe; Knobloch, Edgar

    2006-01-01T23:59:59.000Z

    contrasts and driving strengths µ. The thin lines corresponddriving the typical situation on a homogeneous substrate is as shown in ?gure 3 (dashed line).line (red online). heterogeneity. For very small driving it

  3. U-183: ISC BIND DNS Resource Records Handling Vulnerability

    Broader source: Energy.gov [DOE]

    This problem was uncovered while testing with experimental DNS record types. It is possible to add records to BIND with null (zero length) rdata fields.

  4. acid binding protein: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Richardson, Charles C. 17 Acidic pH Changes Receptor Binding Specificity of Helicobacter pylori: a Binary Adhesion Model in which Surface Heat Shock (Stress) Proteins...

  5. Correlation between fundamental binding forces and clinical prognosis...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Atomic force microscopy was used to “fish” for binding reactions between a fibronectin-coated probe (i.e., substrate simulating an implant device) and each of 15...

  6. Crown Ethers in Graphene Bring Strong, Selective Binding | ornl...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Materials Characterization Crown Ethers in Graphene Bring Strong, Selective Binding November 14, 2014 Schematic showing a graphene sheet containing an array of ideal crown ethers....

  7. Crown Ethers Flatten in Graphene for Strong, Specific Binding...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    SHARE Crown Ethers Flatten in Graphene for Strong, Specific Binding ORNL discovery holds potential for separations, sensors, batteries, biotech and more This sheet of graphene...

  8. antigen papillomavirus binding: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    specificity, the present set of studies undertook similar goals by Corradin; Jacques M. Chiller 1979-01-01 124 Human Erythrocytes Selectively Bind and Enrich Infectious HIV-1...

  9. antigen binding repertoire: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    specificity, the present set of studies undertook similar goals by Corradin; Jacques M. Chiller 1979-01-01 127 Human Erythrocytes Selectively Bind and Enrich Infectious HIV-1...

  10. antigen binds phosphatase: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    specificity, the present set of studies undertook similar goals by Corradin; Jacques M. Chiller 1979-01-01 139 Human Erythrocytes Selectively Bind and Enrich Infectious HIV-1...

  11. Binding Energy of dº Transition Metals to Alkenes By Wave...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Energy of dº Transition Metals to Alkenes By Wave Function Theory and Density Functional Theory. Binding Energy of dº Transition Metals to Alkenes By Wave Function Theory...

  12. atomic binding energy: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Calvin W. Johnson; Joshua T. Staker 2012-08-30 2 Estimating ProteinLigand Binding Free Energy: Atomic Solvation Parameters for Partition Coefficient and Biotechnology...

  13. Coiled transmission line pulse generators

    DOE Patents [OSTI]

    McDonald, Kenneth Fox (Columbia, MO)

    2010-11-09T23:59:59.000Z

    Methods and apparatus are provided for fabricating and constructing solid dielectric "Coiled Transmission Line" pulse generators in radial or axial coiled geometries. The pour and cure fabrication process enables a wide variety of geometries and form factors. The volume between the conductors is filled with liquid blends of monomers, polymers, oligomers, and/or cross-linkers and dielectric powders; and then cured to form high field strength and high dielectric constant solid dielectric transmission lines that intrinsically produce ideal rectangular high voltage pulses when charged and switched into matched impedance loads. Voltage levels may be increased by Marx and/or Blumlein principles incorporating spark gap or, preferentially, solid state switches (such as optically triggered thyristors) which produce reliable, high repetition rate operation. Moreover, these Marxed pulse generators can be DC charged and do not require additional pulse forming circuitry, pulse forming lines, transformers, or an a high voltage spark gap output switch. The apparatus accommodates a wide range of voltages, impedances, pulse durations, pulse repetition rates, and duty cycles. The resulting mobile or flight platform friendly cylindrical geometric configuration is much more compact, light-weight, and robust than conventional linear geometries, or pulse generators constructed from conventional components. Installing additional circuitry may accommodate optional pulse shape improvements. The Coiled Transmission Lines can also be connected in parallel to decrease the impedance, or in series to increase the pulse length.

  14. Camera Calibration Using Line Correspondences

    E-Print Network [OSTI]

    Hartley, Richard

    Camera Calibration Using Line Correspondences Richard I. Hartley G.E. CRD, Schenectady, NY, 12301. The three cameras may have different unknown calibrations, and the essential matrices corresponding to each, camera calibration and scene reconstruction. In the case where all the three cameras are assumed to have

  15. Design of Bcl-2 and Bcl-xL Inhibitors with Subnanomolar Binding Affinities Based upon a New Scaffold

    SciTech Connect (OSTI)

    Zhou, Haibin; Chen, Jianfang; Meagher, Jennifer L.; Yang, Chao-Yie; Aguilar, Angelo; Liu, Liu; Bai, Longchuan; Cong, Xin; Cai, Qian; Fang, Xueliang; Stuckey, Jeanne A.; Wang, Shaomeng (Michigan)

    2014-10-02T23:59:59.000Z

    Employing a structure-based strategy, we have designed a new class of potent small-molecule inhibitors of the anti-apoptotic proteins Bcl-2 and Bcl-xL. An initial lead compound with a new scaffold was designed based upon the crystal structure of Bcl-xL and U.S. Food and Drug Administration (FDA) approved drugs and was found to have an affinity of 100 {micro}M for both Bcl-2 and Bcl-xL. Linking this weak lead to another weak-affinity fragment derived from Abbott's ABT-737 led to an improvement of the binding affinity by a factor of >10,000. Further optimization ultimately yielded compounds with subnanomolar binding affinities for both Bcl-2 and Bcl-xL and potent cellular activity. The best compound (21) binds to Bcl-xL and Bcl-2 with K{sub i} < 1 nM, inhibits cell growth in the H146 and H1417 small-cell lung cancer cell lines with IC{sub 50} values of 60-90 nM, and induces robust cell death in the H146 cancer cell line at 30-100 nM.

  16. Neptunium Binding Kinetics with Arsenazo(III)

    SciTech Connect (OSTI)

    Leigh R. Martin; Aaron T. Johnson; Stephen P. Mezyk

    2014-08-01T23:59:59.000Z

    This document has been prepared to meet FCR&D level 2 milestone M2FT-14IN0304021, “Report on the results of actinide binding kinetics with aqueous phase complexants” This work was carried out under the auspices of the Thermodynamics and Kinetics of Advanced Separations Systems FCR&D work package. The report details kinetics experiments that were performed to measure rates of aqueous phase complexation for pentavalent neptunium with the chromotropic dye Arsenazo III (AAIII). The studies performed were designed to determine how pH, ionic strength and AAIII concentration may affect the rate of the reaction. A brief comparison with hexavalent neptunium is also made. It was identified that as pH was increased the rate of reaction also increased, however increasing the ionic strength and concentration of AAIII had the opposite effect. Interestingly, the rate of reaction of Np(VI) with AAIII was found to be slower than that of the Np(V) reaction.

  17. Nonlinear regression analysis of field emission data

    E-Print Network [OSTI]

    Barry, Scott Wilson

    1992-01-01T23:59:59.000Z

    for the zirconium/tungsten cathode data. . Regressed enhancement fa. ctors using the integral model(solid line) and approximate model(dashed line) over a range of fixed work functions for the zirconium/tungsten cathode data. 70 Integral model(solid line...) and linear( dashed line) fitting curves for the zirconium/tungsten cathode data, . 71 33 Integral model(solid line) and linear(dashed line) fitting curves for the zirconium/tungsten cathode data, excluding the last three suspect data points. 72 CHAPTER I...

  18. Hydrogen Bonding Penalty upon Ligand Binding Hongtao Zhao, Danzhi Huang*

    E-Print Network [OSTI]

    Caflisch, Amedeo

    Hydrogen Bonding Penalty upon Ligand Binding Hongtao Zhao, Danzhi Huang* Department of Biochemistry, University of Zurich, Zurich, Switzerland Abstract Ligand binding involves breakage of hydrogen bonds with water molecules and formation of new hydrogen bonds between protein and ligand. In this work, the change

  19. The DNA binding activity of p53 displays reactiondiffusion kinetics

    E-Print Network [OSTI]

    Hinow, Peter

    The DNA binding activity of p53 displays reaction­diffusion kinetics 26th Southeastern 37240 The DNA binding activity of p53 displays reaction­diffusion kinetics ­ p. 1/2 #12;Collaborators, Vanderbilt University · Emmanuele DiBenedetto, PhD, Department of Mathematics, Vanderbilt University The DNA

  20. Unexpected Nondissociative Binding of N2O on Oxygen Vacancies...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Nondissociative Binding of N2O on Oxygen Vacancies on a Rutile TiO2(110)-1×1 . Unexpected Nondissociative Binding of N2O on Oxygen Vacancies on a Rutile TiO2(110)-1×1 ....

  1. news and views nucleotide binds to the polymerase and

    E-Print Network [OSTI]

    Schedl, Paul

    news and views nucleotide binds to the polymerase and before it is incorporated into DNA. Recent structures of several structurally diverse DNA polymerases complexed to DNA and nucleotide substrates have shown that their active sites adopt a closed conforma- tion upon binding to the correct nucleotide12

  2. Efficient generic on-line/off-line (threshold) signatures without key exposure q

    E-Print Network [OSTI]

    Kim, Kwangjo

    security requirements. Ó 2008 Elsevier Inc. All rights reserved. 1. Introduction The notion of on-line/off-line manufacturing process or as a background computation whenever the card is connected to power, and the on-lineEfficient generic on-line/off-line (threshold) signatures without key exposure q Xiaofeng Chen a

  3. The MICE Muon Beam Line

    SciTech Connect (OSTI)

    Apollonio, Marco [High Energy Physics Group, Department of Physics, Imperial College London SW7 2AZ (United Kingdom)

    2011-10-06T23:59:59.000Z

    In the Muon Ionization Cooling Experiment (MICE) at RAL, muons are produced and transported in a dedicated beam line connecting the production point (target) to the cooling channel. We discuss the main features of the beamline, meant to provide muons with momenta between 140 MeV/c and 240 MeV/c and emittances up to 10 mm rad, which is accomplished by means of a diffuser. Matching procedures to the MICE cooling channel are also described. In summer 2010 we performed an intense data taking campaign to finalize the calibration of the MICE Particle Identification (PID) detectors and the understanding of the beam line, which completes the STEPI phase of MICE. We highlight the main results from these data.

  4. In-line thermoelectric module

    DOE Patents [OSTI]

    Pento, Robert (Algonquin, IL); Marks, James E. (Glenville, NY); Staffanson, Clifford D. (S. Glens Falls, NY)

    2000-01-01T23:59:59.000Z

    A thermoelectric module with a plurality of electricity generating units each having a first end and a second end, the units being arranged first end to second end along an in-line axis. Each unit includes first and second elements each made of a thermoelectric material, an electrically conductive hot member arranged to heat one side of the first element, and an electrically conductive cold member arranged to cool another side of the first element and to cool one side of the second element. The hot member, the first element, the cold member and the second element are supported in a fixture, are electrically connected respectively to provide an electricity generating unit, and are arranged respectively in positions along the in-line axis. The individual components of each generating unit and the respective generating units are clamped in their in-line positions by a loading bolt at one end of the fixture and a stop wall at the other end of the fixture. The hot members may have a T-shape and the cold members an hourglass shape to facilitate heat transfer. The direction of heat transfer through the hot members may be perpendicular to the direction of heat transfer through the cold members, and both of these heat transfer directions may be perpendicular to the direction of current flow through the module.

  5. Capillary electrophoresis-fluorescence line narrowing system (CE-FLNS) for on-line structural characterization

    DOE Patents [OSTI]

    Jankowiak, R.J.; Small, G.J.; Shields, P.A.

    1999-04-27T23:59:59.000Z

    Capillary electrophoresis (CE) is interfaced with low temperature fluorescence line-narrowing (FLN) spectroscopy for on-line structural characterization of separated molecular analytes. 21 figs.

  6. Capillary electrophoresis-fluorescence line narrowing system (CE-FLNS) for on-line structural characterization

    DOE Patents [OSTI]

    Jankowiak, Ryszard J. (Ames, IA); Small, Gerald J. (Ames, IA); Shields, Peter A. (Reading, MA)

    1999-04-27T23:59:59.000Z

    Capillary electrophoresis (CE) is interfaced with low temperature fluorescence line-narrowing (FLN) spectroscopy for on-line structural characterization of separated molecular analytes.

  7. FB Line Basis for Interim Operation

    SciTech Connect (OSTI)

    Shedrow, B.

    1998-10-19T23:59:59.000Z

    The safety analysis of the FB-Line Facility indicates that the operation of FB-Line to support the current mission does not present undue risk to the facility and co-located workers, general public, or the environment.

  8. Structural and Histone Binding Ability Characterizations of Human PWWP Domains

    SciTech Connect (OSTI)

    Wu, Hong; Zeng, Hong; Lam, Robert; Tempel, Wolfram; Amaya, Maria F.; Xu, Chao; Dombrovski, Ludmila; Qiu, Wei; Wang, Yanming; Min, Jinrong (Toronto); (Penn)

    2013-09-25T23:59:59.000Z

    The PWWP domain was first identified as a structural motif of 100-130 amino acids in the WHSC1 protein and predicted to be a protein-protein interaction domain. It belongs to the Tudor domain 'Royal Family', which consists of Tudor, chromodomain, MBT and PWWP domains. While Tudor, chromodomain and MBT domains have long been known to bind methylated histones, PWWP was shown to exhibit histone binding ability only until recently. The PWWP domain has been shown to be a DNA binding domain, but sequence analysis and previous structural studies show that the PWWP domain exhibits significant similarity to other 'Royal Family' members, implying that the PWWP domain has the potential to bind histones. In order to further explore the function of the PWWP domain, we used the protein family approach to determine the crystal structures of the PWWP domains from seven different human proteins. Our fluorescence polarization binding studies show that PWWP domains have weak histone binding ability, which is also confirmed by our NMR titration experiments. Furthermore, we determined the crystal structures of the BRPF1 PWWP domain in complex with H3K36me3, and HDGF2 PWWP domain in complex with H3K79me3 and H4K20me3. PWWP proteins constitute a new family of methyl lysine histone binders. The PWWP domain consists of three motifs: a canonical {beta}-barrel core, an insertion motif between the second and third {beta}-strands and a C-terminal {alpha}-helix bundle. Both the canonical {beta}-barrel core and the insertion motif are directly involved in histone binding. The PWWP domain has been previously shown to be a DNA binding domain. Therefore, the PWWP domain exhibits dual functions: binding both DNA and methyllysine histones.

  9. Chromospheric Dynamics and Line Formation

    E-Print Network [OSTI]

    R. Hammer; P. Ulmschneider

    2007-07-14T23:59:59.000Z

    The solar chromosphere is very dynamic, due to the presence of large amplitude hydrodynamic waves. Their propagation is affected by NLTE radiative transport in strong spectral lines, which can in turn be used to diagnose the dynamics of the chromosphere. We give a basic introduction into the equations of NLTE radiation hydrodynamics and describe how they are solved in current numerical simulations. The comparison with observation shows that one-dimensional codes can describe strong brightenings quite well, but the overall chromospheric dynamics appears to be governed by three-dimensional shock propagation.

  10. Line-focus sun trackers

    SciTech Connect (OSTI)

    Gee, R.

    1980-05-01T23:59:59.000Z

    Sun trackers have been a troublesome component for line-focus concentrating collector systems. The problems have included poor accuracy, component failures, false locks on clouds, and restricted tracker operating ranges. In response to these tracking difficulties, a variety of improved sun trackers have been developed. A testing program is underway at SERI to determine the tracking accuracy of this new generation of sun trackers. The three major types of trackers are defined, some recent sun tracker developments are described, and the testing that is underway is outlined.

  11. Radiative transfer in molecular lines

    E-Print Network [OSTI]

    A. Asensio Ramos; J. Trujillo Bueno; J. Cernicharo

    2001-02-15T23:59:59.000Z

    The highly convergent iterative methods developed by Trujillo Bueno and Fabiani Bendicho (1995) for radiative transfer (RT) applications are generalized to spherical symmetry with velocity fields. These RT methods are based on Jacobi, Gauss-Seidel (GS), and SOR iteration and they form the basis of a new NLTE multilevel transfer code for atomic and molecular lines. The benchmark tests carried out so far are presented and discussed. The main aim is to develop a number of powerful RT tools for the theoretical interpretation of molecular spectra.

  12. ShaRE Credit Line

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742EnergyOnItemResearch > TheNuclearHomelandMultivariate Metal-Organic FrameworksCredit Line

  13. Line Management Perspective: National Nuclear Security Administration...

    Broader source: Energy.gov (indexed) [DOE]

    National Nuclear Security Administration (NNSA) Line Management Perspective: National Nuclear Security Administration (NNSA) Addthis Description Slide Presentation by Jim...

  14. Methods of Using Existing Wire Lines (power lines, phone lines, internet lines) for Totally Secure Classical Communication Utilizing Kirchoff's Law and Johnson-like Noise

    E-Print Network [OSTI]

    Laszlo B. Kish

    2006-10-02T23:59:59.000Z

    We outline some general solutions to use already existing and currently used wire lines, such as power lines, phone lines, internet lines, etc, for the unconditionally secure communication method based on Kirchoff's Law and Johnson-like Noise (KLJN). Two different methods are shown. One is based on filters used at single wires and the other one utilizes a common mode voltage superimposed on a three-phase powerline.

  15. Methods of Using Existing Wire Lines (power lines, phone lines, internet lines) for Totally Secure Classical Communication Utilizing Kirchoff's Law and Johnson-like Noise

    E-Print Network [OSTI]

    Kish, L B

    2006-01-01T23:59:59.000Z

    We outline some general solutions to use already existing and currently used wire lines, such as power lines, phone lines, internet lines, etc, for the unconditionally secure communication method based on Kirchoff's Law and Johnson-like Noise (KLJN). Two different methods are shown. One is based on filters used at single wires and the other one utilizes a common mode voltage superimposed on a three-phase powerline.

  16. Discontinuities in transmission lines and wave guides

    E-Print Network [OSTI]

    Mathis, Harold Fletcher

    1952-01-01T23:59:59.000Z

    DISCONTINUITIES IN TRANSMISSION LINES AND WAVEGUIDES H. F. MATHIS DISCONTINUITIES IN TRANSMISSION LINES AND liVAVNGUIDBS A Thesis By Harola Fletcher htathis Approved as to style and content by: Chairman of Committee DISCONTINUITIES... Subject. Electrical Engineering COHERENT S PAGE I Introduct ion, II Dl. scontlnuit les in Transmission Lines 2 1. Some Seneral char aeter 1 st 1 ca of the transmission lines. . . , . . . , ~ 5 2 ~ 2. Types of dlscontlnuitles ln transmls- sl...

  17. Neuronal differentiation modulates the dystrophin Dp71d binding to the nuclear matrix

    SciTech Connect (OSTI)

    Rodriguez-Munoz, Rafael; Villarreal-Silva, Marcela; Gonzalez-Ramirez, Ricardo [Departamento de Genetica y Biologia Molecular, Centro de Investigacion y de Estudios Avanzados del IPN, Avenida Instituto Politecnico Nacional 2508, Apartado Postal 14-740, C.P. 07360, Mexico D.F. (Mexico); Garcia-Sierra, Francisco [Departamento de Biologia Celular, Centro de Investigacion y de Estudios Avanzados del IPN, Avenida Instituto Politecnico Nacional 2508, Apartado Postal 14-740, C.P. 07360, Mexico D.F. (Mexico); Mondragon, Monica; Mondragon, Ricardo [Departamento de Bioquimica, Centro de Investigacion y de Estudios Avanzados del IPN, Avenida Instituto Politecnico Nacional 2508, Apartado Postal 14-740, C.P. 07360, Mexico D.F. (Mexico); Cerna, Joel [Facultad de Medicina, Universidad de Colima, Avenida Universidad 333. C.P. 28040 Colima, Col. (Mexico); Cisneros, Bulmaro [Departamento de Genetica y Biologia Molecular, Centro de Investigacion y de Estudios Avanzados del IPN, Avenida Instituto Politecnico Nacional 2508, Apartado Postal 14-740, C.P. 07360, Mexico D.F. (Mexico)], E-mail: bcisnero@cinvestav.mx

    2008-10-24T23:59:59.000Z

    The function of dystrophin Dp71 in neuronal cells remains unknown. To approach this issue, we have selected the PC12 neuronal cell line. These cells express both a Dp71f cytoplasmic variant and a Dp71d nuclear isoform. In this study, we demonstrated by electron and confocal microscopy analyses of in situ nuclear matrices and Western blotting evaluation of cell extracts that Dp71d associates with the nuclear matrix. Interestingly, this binding is modulated during NGF-induced neuronal differentiation of PC12 cells with a twofold increment in the differentiated cells, compared to control cells. Also, distribution of Dp71d along the periphery of the nuclear matrix observed in the undifferentiated cells is replaced by intense fluorescent foci localized in Center of the nucleoskeletal structure. In summary, we revealed that Dp71d is a dynamic component of nuclear matrix that might participate in the nuclear modeling occurring during neuronal differentiation.

  18. A Roundtable Overview Driving the Top Line

    E-Print Network [OSTI]

    A Roundtable Overview Driving the Top Line #12;© 2012 Glassmeyer/McNamee Center for Digital of the Center for Digital Strategies. Driving the Top Line A Thought Leadership Roundtable on Digital, Switzerland for a discussion on how information technology can help companies drive top-line revenue. In many

  19. Nuclear binding energy and symmetry energy of nuclear matter with modern nucleon-nucleon potentials

    SciTech Connect (OSTI)

    Hassaneen, Kh.S.A., E-mail: khs_94@yahoo.com [Physics Department, Faculty of Science, Sohag University, Sohag (Egypt); Abo-Elsebaa, H.M.; Sultan, E.A. [Physics Department, Faculty of Science, Sohag University, Sohag (Egypt); Mansour, H.M.M. [Physics Department, Faculty of Science, Cairo University, Giza (Egypt)

    2011-03-15T23:59:59.000Z

    Research Highlights: > The nuclear matter is studied within the Brueckner-Hartree-Fock (BHF) approach employing the most recent accurate nucleon-nucleon potentials. > The results come out by approximating the single particle self-consistent potential with a parabolic form. > We discuss the current status of the Coester line, i.e., density and energy of the various saturation points being strongly linearly correlated. > The nuclear symmetry energy is calculated as the difference between the binding energy of pure neutron matter and that of symmetric nuclear matter. - Abstract: The binding energy of nuclear matter at zero temperature in the Brueckner-Hartree-Fock approximation with modern nucleon-nucleon potentials is studied. Both the standard and continuous choices of single particle energies are used. These modern nucleon-nucleon potentials fit the deuteron properties and are phase shifts equivalent. Comparison with other calculations is made. In addition we present results for the symmetry energy obtained with different potentials, which is of great importance in astrophysical calculation.

  20. X-ray Crystallographic Studies of Substrate Binding to Aristolochene Synthase Suggest a Metal Ion Binding Sequence for Catalysis

    SciTech Connect (OSTI)

    Shishova,E.; Yu, F.; Miller, D.; Faraldos, J.; Zhao, Y.; Coates, R.; Allemann, R.; Cane, D.; Christianson, D.

    2008-01-01T23:59:59.000Z

    The universal sesquiterpene precursor, farnesyl diphosphate (FPP), is cyclized in an Mg2+-dependent reaction catalyzed by the tetrameric aristolochene synthase from Aspergillus terreus to form the bicyclic hydrocarbon aristolochene and a pyrophosphate anion (PPi) coproduct. The 2.1- Angstroms resolution crystal structure determined from crystals soaked with FPP reveals the binding of intact FPP to monomers A-C, and the binding of PPi and Mg2+B to monomer D. The 1.89- Angstroms resolution structure of the complex with 2-fluorofarnesyl diphosphate (2F-FPP) reveals 2F-FPP binding to all subunits of the tetramer, with Mg2+Baccompanying the binding of this analogue only in monomer D. All monomers adopt open activesite conformations in these complexes, but slight structural changes in monomers C and D of each complex reflect the very initial stages of a conformational transition to the closed state. Finally, the 2.4- Angstroms resolution structure of the complex with 12,13-difluorofarnesyl diphosphate (DF-FPP) reveals the binding of intact DF-FPP to monomers A-C in the open conformation and the binding of PPi, Mg2+B, and Mg2+C to monomer D in a predominantly closed conformation. Taken together, these structures provide 12 independent 'snapshots' of substrate or product complexes that suggest a possible sequence for metal ion binding and conformational changes required for catalysis.

  1. Cross delay line sensor characterization

    SciTech Connect (OSTI)

    Owens, Israel J [Los Alamos National Laboratory; Remelius, Dennis K [Los Alamos National Laboratory; Tiee, Joe J [Los Alamos National Laboratory; Buck, Steven E [Los Alamos National Laboratory; Whittemore, Stephen R [Los Alamos National Laboratory; Thompson, David C [Los Alamos National Laboratory; Shirey, Robert [Los Alamos National Laboratory

    2010-01-01T23:59:59.000Z

    There exists a wealth of information in the scientific literature on the physical properties and device characterization procedures for complementary metal oxide semiconductor (CMOS), charge coupled device (CCD) and avalanche photodiode (APD) format detectors. Numerous papers and books have also treated photocathode operation in the context of photomultiplier tube (PMT) operation for either non imaging applications or limited night vision capability. However, much less information has been reported in the literature about the characterization procedures and properties of photocathode detectors with novel cross delay line (XDL) anode structures. These allow one to detect single photons and create images by recording space and time coordinate (X, Y & T) information. In this paper, we report on the physical characteristics and performance of a cross delay line anode sensor with an enhanced near infrared wavelength response photocathode and high dynamic range micro channel plate (MCP) gain (> 10{sup 6}) multiplier stage. Measurement procedures and results including the device dark event rate (DER), pulse height distribution, quantum and electronic device efficiency (QE & DQE) and spatial resolution per effective pixel region in a 25 mm sensor array are presented. The overall knowledge and information obtained from XDL sensor characterization allow us to optimize device performance and assess capability. These device performance properties and capabilities make XDL detectors ideal for remote sensing field applications that require single photon detection, imaging, sub nano-second timing response, high spatial resolution (10's of microns) and large effective image format.

  2. Phospholamban mutants compete with wild type for SERCA binding in living cells

    SciTech Connect (OSTI)

    Gruber, Simon J.; Haydon, Suzanne [Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota Medical School, Minneapolis, MN 55455 (United States)] [Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota Medical School, Minneapolis, MN 55455 (United States); Thomas, David D., E-mail: ddt@umn.edu [Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota Medical School, Minneapolis, MN 55455 (United States)

    2012-04-06T23:59:59.000Z

    Highlights: Black-Right-Pointing-Pointer PLB phosphorylation in HEK cells increased FRET between YFP-PLB and CFP-SERCA. Black-Right-Pointing-Pointer Competition: Expressing loss-of-function PLB mutants in the system decreased FRET. Black-Right-Pointing-Pointer The FRET assay could screen potential therapeutic PLB mutants to activate SERCA. -- Abstract: We have used fluorescent fusion proteins stably expressed in HEK cells to detect directly the interaction between the sarcoplasmic reticulum Ca-ATPase (SERCA) and phospholamban (PLB) in living cells, in order to design PLB mutants for gene therapy. Ca{sup 2+} cycling in muscle cells depends strongly on SERCA. Heart failure (HF), which contributes to 12% of US deaths, typically exhibits decreased SERCA activity, and several potential therapies for HF aim to increase SERCA activity. We are investigating the use of LOF-PLB mutants (PLB{sub M}) as gene therapy vectors to increase SERCA activity. Active SERCA1a and WT-PLB, tagged at their N termini with fluorescent proteins (CFP and YFP), were coexpressed in stable HEK cell lines, and fluorescence resonance energy transfer (FRET) was used to detect their interaction directly. Phosphorylation of PLB, induced by forskolin, caused an increase in FRET from CFP-SERCA to YFP-PLB, indicating that SERCA inhibition can be relieved without dissociation of the complex. This suggests that a LOF mutant might bind to SERCA with sufficient affinity to complete effectively with WT-PLB, thus relieving SERCA inhibition. Therefore, we transiently expressed a series of PLB{sub M} in the CFP-SERCA/YFP-PLB cell line, and found decreased FRET, implying competition between PLB{sub M} and WT-PLB for binding to SERCA. These results establish this FRET assay as a rapid and quantitative means of screening PLB{sub M} for optimization of gene therapy to activate SERCA, as needed for gene therapy in HF.

  3. DB-PABP: a database of polyanion-binding proteins

    E-Print Network [OSTI]

    Fang, Jianwen; Dong, Yinghua; Slamat-Miller, Nazila; Middaugh, C. Russell

    2007-10-04T23:59:59.000Z

    The interactions between polyanions (PAs) and polyanion-binding proteins (PABPs) have been found to play significant roles in many essential biological processes including intracellular organization, transport and protein folding. Furthermore, many...

  4. Research paper Drug diffusion and binding in ionizable interpenetrating networks

    E-Print Network [OSTI]

    Peppas, Nicholas A.

    Research paper Drug diffusion and binding in ionizable interpenetrating networks from poly) (PVA), poly(acrylic acid) (PAA), and their interpenetrating networks (IPNs) were prepared using by measuring their equilibrium polymer volume fraction, equilibrium swelling ratio, and mesh size. Drug

  5. Original article Distribution of endogenous retinoids, retinoid binding

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    of specific retinoid-binding proteins was investigated; these are involved in vitamin A transport, metabolism is induced by a concentration gradient (high posteri- orly) of all-trans-RA along the anterior-p

  6. Understanding regulation of mRNA by RNA binding proteins

    E-Print Network [OSTI]

    Robertson, Alexander De Jong

    2014-01-01T23:59:59.000Z

    Posttranscriptional regulation of mRNA by RNA-binding proteins plays key roles in regulating the transcriptome over the course of development, between tissues and in disease states. The specific interactions between mRNA ...

  7. acid inhibitor binding: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of correctly predicted inter- face residues in actual interface residues Zhou, Yaoqi 162 Sm-like protein Hfq: Location of the ATP-binding site and the effect of ATP on HfqRNA...

  8. atp binding protein: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    corroborate asymmetry of catalysis in F0F1-ATP synthase. Zarrabi, Nawid; Diez, Manuel; Graeber, Peter; Wrachtrup, Joerg; Boersch, Michael 2007-01-01 408 Interplay of pH and Binding...

  9. Metal binding proteins, recombinant host cells and methods

    DOE Patents [OSTI]

    Summers, Anne O.; Caguiat, Jonathan J.

    2004-06-15T23:59:59.000Z

    The present disclosure provides artificial heavy metal binding proteins termed chelons by the inventors. These chelons bind cadmium and/or mercuric ions with relatively high affinity. Also disclosed are coding sequences, recombinant DNA molecules and recombinant host cells comprising those recombinant DNA molecules for expression of the chelon proteins. In the recombinant host cells or transgenic plants, the chelons can be used to bind heavy metals taken up from contaminated soil, groundwater or irrigation water and to concentrate and sequester those ions. Recombinant enteric bacteria can be used within the gastrointestinal tracts of animals or humans exposed to toxic metal ions such as mercury and/or cadmium, where the chelon recombinantly expressed in chosen in accordance with the ion to be rededicated. Alternatively, the chelons can be immobilized to solid supports to bind and concentrate heavy metals from a contaminated aqueous medium including biological fluids.

  10. angiotensin binding site: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ser-142 (14). Ser-129 is believed to be a substrate for glycogen synthase kinase 3 Brownfield, Mark S. 55 Similarity Analysis of Protein Binding Sites: A Generalization of the...

  11. antigen binding site: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ser-142 (14). Ser-129 is believed to be a substrate for glycogen synthase kinase 3 Brownfield, Mark S. 69 Similarity Analysis of Protein Binding Sites: A Generalization of the...

  12. alcohol binding sites: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ser-142 (14). Ser-129 is believed to be a substrate for glycogen synthase kinase 3 Brownfield, Mark S. 56 Similarity Analysis of Protein Binding Sites: A Generalization of the...

  13. albumin binding sites: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ser-142 (14). Ser-129 is believed to be a substrate for glycogen synthase kinase 3 Brownfield, Mark S. 54 Similarity Analysis of Protein Binding Sites: A Generalization of the...

  14. anesthetic binding site: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ser-142 (14). Ser-129 is believed to be a substrate for glycogen synthase kinase 3 Brownfield, Mark S. 57 Similarity Analysis of Protein Binding Sites: A Generalization of the...

  15. acid binding sites: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ser-142 (14). Ser-129 is believed to be a substrate for glycogen synthase kinase 3 Brownfield, Mark S. 82 Similarity Analysis of Protein Binding Sites: A Generalization of the...

  16. aml-1 binding site: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ser-142 (14). Ser-129 is believed to be a substrate for glycogen synthase kinase 3 Brownfield, Mark S. 55 Similarity Analysis of Protein Binding Sites: A Generalization of the...

  17. affinity binding sites: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ser-142 (14). Ser-129 is believed to be a substrate for glycogen synthase kinase 3 Brownfield, Mark S. 112 Similarity Analysis of Protein Binding Sites: A Generalization of the...

  18. antagonist binding sites: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ser-142 (14). Ser-129 is believed to be a substrate for glycogen synthase kinase 3 Brownfield, Mark S. 58 Similarity Analysis of Protein Binding Sites: A Generalization of the...

  19. alcohol binding site: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ser-142 (14). Ser-129 is believed to be a substrate for glycogen synthase kinase 3 Brownfield, Mark S. 56 Similarity Analysis of Protein Binding Sites: A Generalization of the...

  20. androgen binding protein: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ser-142 (14). Ser-129 is believed to be a substrate for glycogen synthase kinase 3 Brownfield, Mark S. 135 A High-Throughput Solid-Phase Microplate Protein-Binding Assay to...

  1. antidepressant binding site: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ser-142 (14). Ser-129 is believed to be a substrate for glycogen synthase kinase 3 Brownfield, Mark S. 55 Similarity Analysis of Protein Binding Sites: A Generalization of the...

  2. antibody binding site: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ser-142 (14). Ser-129 is believed to be a substrate for glycogen synthase kinase 3 Brownfield, Mark S. 75 Binding of HIV-1 gp41-Directed Neutralizing and Non-Neutralizing...

  3. autoantibody binding site: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ser-142 (14). Ser-129 is believed to be a substrate for glycogen synthase kinase 3 Brownfield, Mark S. 56 Similarity Analysis of Protein Binding Sites: A Generalization of the...

  4. atp binding site: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ser-142 (14). Ser-129 is believed to be a substrate for glycogen synthase kinase 3 Brownfield, Mark S. 90 Similarity Analysis of Protein Binding Sites: A Generalization of the...

  5. auxin binding protein: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ser-142 (14). Ser-129 is believed to be a substrate for glycogen synthase kinase 3 Brownfield, Mark S. 150 A High-Throughput Solid-Phase Microplate Protein-Binding Assay to...

  6. acid binding site: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ser-142 (14). Ser-129 is believed to be a substrate for glycogen synthase kinase 3 Brownfield, Mark S. 82 Similarity Analysis of Protein Binding Sites: A Generalization of the...

  7. androgen binding sites: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ser-142 (14). Ser-129 is believed to be a substrate for glycogen synthase kinase 3 Brownfield, Mark S. 55 Similarity Analysis of Protein Binding Sites: A Generalization of the...

  8. affinity binding site: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ser-142 (14). Ser-129 is believed to be a substrate for glycogen synthase kinase 3 Brownfield, Mark S. 112 Similarity Analysis of Protein Binding Sites: A Generalization of the...

  9. a1 binding site: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ser-142 (14). Ser-129 is believed to be a substrate for glycogen synthase kinase 3 Brownfield, Mark S. 59 Similarity Analysis of Protein Binding Sites: A Generalization of the...

  10. amp binding proteins: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ser-142 (14). Ser-129 is believed to be a substrate for glycogen synthase kinase 3 Brownfield, Mark S. 4 Volume 159, number 1,2 FEBS 0638 August 1983 Specific DNA binding of the...

  11. agonist binding site: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ser-142 (14). Ser-129 is believed to be a substrate for glycogen synthase kinase 3 Brownfield, Mark S. 62 Similarity Analysis of Protein Binding Sites: A Generalization of the...

  12. assisted binding site: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ser-142 (14). Ser-129 is believed to be a substrate for glycogen synthase kinase 3 Brownfield, Mark S. 54 Similarity Analysis of Protein Binding Sites: A Generalization of the...

  13. allosteric binding sites: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ser-142 (14). Ser-129 is believed to be a substrate for glycogen synthase kinase 3 Brownfield, Mark S. 68 Similarity Analysis of Protein Binding Sites: A Generalization of the...

  14. allosteric binding site: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ser-142 (14). Ser-129 is believed to be a substrate for glycogen synthase kinase 3 Brownfield, Mark S. 68 Similarity Analysis of Protein Binding Sites: A Generalization of the...

  15. activator inhibitor-1 binding: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ser-142 (14). Ser-129 is believed to be a substrate for glycogen synthase kinase 3 Brownfield, Mark S. 147 Stress modulation of visuomotor binding CiteSeer Summary: The primate...

  16. The continental margin is a key source of iron to the HNLC North Pacific Ocean

    E-Print Network [OSTI]

    Lam, P.J.

    2008-01-01T23:59:59.000Z

    line), and by synchrotron XRF (total) (dashed lines, greycomparison. Error bars on XRF Fe, where present, indicatesynchrotron x-ray fluorescence (XRF) at beamline 10.3.2 at

  17. Towards engineering hormone-binding globulins as drug delivery agents

    E-Print Network [OSTI]

    Chan, Wee Lee; Zhou, Aiwu; Read, Randy J.

    2014-11-26T23:59:59.000Z

    in optical thickness of the sensor tip. Cortisol was immobilised on the biosensor tip surface by forming an adduct of hydrocortisone 21-hemisuccinate and a pentylamine-biotin linker through an amide bond, then allowing the biotin moiety on this cortisol... adduct to bind to the streptavidin-conjugated biosensor tip. The cortisol- Towards Engineering Hormone-Binding Globulins as Drug Delivery Agents PLOS ONE | DOI:10.1371/journal.pone.0113402 November 26, 2014 4 / 21 conjugated sensors were immersed...

  18. Unsplit bipolar pulse forming line

    DOE Patents [OSTI]

    Rhodes, Mark A. (Pleasanton, CA)

    2011-05-24T23:59:59.000Z

    A bipolar pulse forming transmission line module and system for linear induction accelerators having first, second, third, and fourth planar conductors which form a sequentially arranged interleaved stack having opposing first and second ends, with dielectric layers between the conductors. The first and second planar conductors are connected to each other at the first end, and the first and fourth planar conductors are connected to each other at the second end via a shorting plate. The third planar conductor is electrically connectable to a high voltage source, and an internal switch functions to short at the first end a high voltage from the third planar conductor to the fourth planar conductor to produce a bipolar pulse at the acceleration axis with a zero net time integral. Improved access to the switch is enabled by an aperture through the shorting plate and the proximity of the aperture to the switch.

  19. Magnetically insulated transmission line oscillator

    DOE Patents [OSTI]

    Bacon, Larry D. (Albuquerque, NM); Ballard, William P. (Albuquerque, NM); Clark, M. Collins (Albuquerque, NM); Marder, Barry M. (Albuquerque, NM)

    1988-01-01T23:59:59.000Z

    A magnetically insulated transmission line oscillator employs self-generated magnetic fields to generate microwave energy. An anode of the oscillator includes slow-wave structures which are formed of a plurality of thin conductive vanes defining cavities therebetween, and a gap is formed between the anode and a cathode of the oscillator. In response to a pulsed voltage applied to the anode and cathode, self-generated magnetic fields arfe produced in a cross-field orientation with respect to the orientation of the electric field between the anode and the cathode. The cross-field magnetic fields insulate the flow of electrons in the gap and confine the flow of electrons within the gap.

  20. Gamma-Ray Burst Lines

    E-Print Network [OSTI]

    Michael S. Briggs

    1999-10-20T23:59:59.000Z

    The evidence for spectral features in gamma-ray bursts is summarized. As a guide for evaluating the evidence, the properties of gamma-ray detectors and the methods of analyzing gamma-ray spectra are reviewed. In the 1980's, observations indicated that absorption features below 100 keV were present in a large fraction of bright gamma-ray bursts. There were also reports of emission features around 400 keV. During the 1990's the situation has become much less clear. A small fraction of bursts observed with BATSE have statistically significant low-energy features, but the reality of the features is suspect because in several cases the data of the BATSE detectors appear to be inconsistent. Furthermore, most of the possible features appear in emission rather than the expected absorption. Analysis of data from other instruments has either not been finalized or has not detected lines.

  1. Superconducting transmission line particle detector

    DOE Patents [OSTI]

    Gray, Kenneth E. (Naperville, IL)

    1989-01-01T23:59:59.000Z

    A microvertex particle detector for use in a high energy physic collider including a plurality of parallel superconducting thin film strips separated from a superconducting ground plane by an insulating layer to form a plurality of superconducting waveguides. The microvertex particle detector indicates passage of a charged subatomic particle by measuring a voltage pulse measured across a superconducting waveguide caused by the transition of the superconducting thin film strip from a superconducting to a non-superconducting state in response to the passage of a charged particle. A plurality of superconducting thin film strips in two orthogonal planes plus the slow electromagnetic wave propogating in a superconducting transmission line are used to resolve N.sup.2 ambiguity of charged particle events.

  2. Superconducting transmission line particle detector

    DOE Patents [OSTI]

    Gray, K.E.

    1988-07-28T23:59:59.000Z

    A microvertex particle detector for use in a high energy physic collider including a plurality of parallel superconducting thin film strips separated from a superconducting ground plane by an insulating layer to form a plurality of superconducting waveguides. The microvertex particle detector indicates passage of a charged subatomic particle by measuring a voltage pulse measured across a superconducting waveguide caused by the transition of the superconducting thin film strip from a superconducting to a non- superconducting state in response to the passage of a charged particle. A plurality of superconducting thin film strips in two orthogonal planes plus the slow electromagnetic wave propagating in a superconducting transmission line are used to resolve N/sup 2/ ambiguity of charged particle events. 6 figs.

  3. MANAGING TIGHT BINDING RECEPTORS FOR NEW SPEARATIONS TECHNOLOGIES

    SciTech Connect (OSTI)

    DARYLE H BUSCH RICHARD S GIVENS

    2004-12-10T23:59:59.000Z

    Much of the earth's pollution involves compounds of the metallic elements, including actinides, strontium, cesium, technetium, and RCRA metals. Metal ions bind to molecules called ligands, which are the molecular tools that can manipulate the metal ions under most conditions. This DOE-EMSP sponsored program strives (1) to provide the foundations for using the most powerful ligands in transformational separations technologies and (2) to produce seminal examples of their applications to separations appropriate to the DOE EM mission. These ultra tight-binding ligands can capture metal ions in the most competitive of circumstances (from mineralized sites, lesser ligands, and even extremely dilute solutions), but they react so slowly that they are useless in traditional separations methodologies. Two attacks on this problem are underway. The first accommodates to the challenging molecular lethargy by developing a seminal slow separations methodology termed the soil poultice. The second designs ligands that are only tight-binding while wrapped around the targeted metal ion, but can be put in place by switch-binding and removed by switch-release. We envision a kind of molecular switching process to accelerate the union between metal ion and tight-binding ligand. Molecular switching processes are suggested for overcoming the slow natural equilibration rate with which ultra tight-binding ligands combine with metal ions. Ligands that bind relatively weakly combine with metal ions rapidly, so the trick is to convert a ligand from a weak, rapidly binding species to a powerful, slow releasing ligand--during the binding of the ligand to the metal ion. Such switch-binding ligands must react with themselves, and the reaction must take place under the influence of the metal ion. For example, our generation 1 ligands showed that a well-designed linear ligand with ends that readily combine, forms a cyclic molecule when it wraps around a metal ion. Our generation 2 ligands are even more interesting. They convert from rings to structures that wrap around a metal ion to form a cage. These ligands are called cryptands. Switch release is accomplished by photolytic cleavage of a bond to convert a cyclic ligand into a linear ligand or to break similar bonds in a cryptate. Our studies have demonstrated switch binding and switch release with cryptates of calcium. These remarkable cyclic ligands and cage-like ligands are indeed tight-binding and may, in principle, be incorporated in various separations methodologies, including the soil poultice. The soil poultice mimics the way in which microbes secrete extremely powerful ligands into the soil in order to harvest iron. The cellular membrane of the microbe recognizes the iron/ligand complex and admits it into the cell. The soil poultice uses molecularly imprinted polymers (MIPs) to play the role of the cellular membrane. Imprinting involves creation of the polymer in the presence of the metal/ligand complex. In principle, a well design ligand/MIP combination can be highly selective toward almost any targeted metal ion. The principles for that design are the focus of these investigations. An imprinting molecule can interact with the polymer through any, some, or all of the so-called supramolecular modes; e.g., hydrogen bonding, electrostatic charge, minor ligand bonding, Pi-Pi stacking, and hydrophobic and van der Waals interactions. Historically these modes of binding have given MIPs only small re-binding capacities and very limited selectivities. This program has shown that each mode of interaction can be made more powerful than previously suspected and that combinations of different supramolecular interaction modes can produce remarkable synergisms. The results of this systematic study provide a firm foundation for tailoring molecular imprinted polymers for reclamation of specific metal ion, including those important to the DOE EM mission.

  4. NSLS-II Transport Line Progress

    SciTech Connect (OSTI)

    Fliller R. P.; Wahl, W.; Anderson, A.; Benish, B.; DeBoer, W.; Ganetis, G.; Heese, R.; Hseuh, H.-C.; Hu, J.-P.; Johanson, M.P.; Kosciuk, B.N.; Padrazo, D.; Roy, K.; Shaftan, T.; Singh, O.; Tuozzolo, J.; Wang, G.

    2012-05-20T23:59:59.000Z

    The National Synchrotron Light Source II (NSLS-II) is a state-of-the-art 3-GeV third generation light source currently under construction at Brookhaven National Laboratory. The NSLS-II injection system consists of a 200 MeV linac, a 3-GeV booster synchrotron and associated transfer lines. The first part of the Linac to Booster Transport (LBT) line has been installed for linac commissioning. This part includes all components necessary to commission the NSLS-II linac. The second part of this transport line is undergoing installation. Initial results of hardware commissioning will be discussed. The Booster to Storage Ring (BSR) transport line underwent a design review. The first part of the BSR transport line, consisting of all components necessary to commission the booster will be installed in 2012 for booster commissioning. We report on the final design of the BSR line along with the plan to commission the booster.

  5. Gamma-Ray Line Observations with RHESSI

    E-Print Network [OSTI]

    David M. Smith

    2004-04-30T23:59:59.000Z

    The Reuven Ramaty High Energy Solar Spectroscopic Imager (RHESSI) has been observing gamma-ray lines from the Sun and the Galaxy since its launch in February 2002. Here I summarize the status of RHESSI observations of solar lines (nuclear de-excitation, neutron capture, and positron annihilation), the lines of $^{26}$Al and $^{60}$Fe from the inner Galaxy, and the search for positron annihilation in novae.

  6. DOE Sustainability Reporting Open Line Help Call

    Broader source: Energy.gov [DOE]

    The Sustainability Performance Office (SPO) sponsors open line help calls to assist DOE sites and national laboratories with the annual sustainability reporting process. Representatives from the...

  7. Rubber linings answer to many problems

    SciTech Connect (OSTI)

    Mehra, L. [Quality Linings Co., Aurora, CO (United States)

    1998-12-31T23:59:59.000Z

    The uses of rubber linings in different fields industries are discussed.The physical properties of rubber both natural and synthetic rubber are listed and their importance is evaluated. The aging of rubber is discussed in detail, including effects of temperature on aging of rubber. By virtue of its inherent elasticity and chemical resistance, rubber linings have found many uses in the protection of mining equipment, water treatment tanks and vessels, flue gas desulfurization equipment in power plants and varied process and storage vessels in chemical industries. Rubber has found extensive use in civil engineering field as expansion joints and bladders in dams. Electrical resistance of rubber is useful for its application as an insulating material. Rubber is chemically resistant to acids, alkalies and many salt solutions. Rubber linings are therefore used for protection of steel against these acids, alkalies or salt solutions. The extreme elasticity of rubber has been found useful in its application as a lining material in areas subject to high abrasion. Frequently rubber linings are the linings of choice when a combination of abrasion and chemical attack are to be protected against. Constantly, new formulations of rubber lining compounds are being developed just as new chemical processes are being made. The flexibility of compounding and the relative ease of putting layers of different rubber formulations together in multilayered formulations of rubber lining compounds is leading to new uses of this lining material.

  8. Transmission Line Security Monitor: Final Report

    SciTech Connect (OSTI)

    John Svoboda

    2011-04-01T23:59:59.000Z

    The Electric Power Transmission Line Security Monitor System Operational Test is a project funded by the Technical Support Working Group (TSWG). TSWG operates under the Combating Terrorism Technical Support Office that functions under the Department of Defense. The Transmission Line Security Monitor System is based on technology developed by Idaho National Laboratory. The technology provides a means for real-time monitoring of physical threats and/or damage to electrical transmission line towers and conductors as well as providing operational parameters to transmission line operators to optimize transmission line operation. The end use is for monitoring long stretches of transmission lines that deliver electrical power from remote generating stations to cities and industry. These transmission lines are generally located in remote transmission line corridors where security infrastructure may not exist. Security and operational sensors in the sensor platform on the conductors take power from the transmission line and relay security and operational information to operations personnel hundreds of miles away without relying on existing infrastructure. Initiated on May 25, 2007, this project resulted in pre-production units tested in realistic operational environments during 2010. A technology licensee, Lindsey Manufacturing of Azusa California, is assisting in design, testing, and ultimately production. The platform was originally designed for a security monitoring mission, but it has been enhanced to include important operational features desired by electrical utilities.

  9. Emergency Management Lines of Inquiry, April 2008

    Broader source: Energy.gov (indexed) [DOE]

    Inspection Criteria, Activities, and Lines of Inquiry Functional Area: Hazards Survey (HS) and Emergency Planning Hazards Assessment (EPHA) Inspection Criteria: Emergency...

  10. Detonator comprising a nonlinear transmission line

    DOE Patents [OSTI]

    Elizondo-Decanini, Juan M

    2014-12-30T23:59:59.000Z

    Detonators are described herein. In a general embodiment, the detonator includes a nonlinear transmission line that has a variable capacitance. Capacitance of the nonlinear transmission line is a function of voltage on the nonlinear transmission line. The nonlinear transmission line receives a voltage pulse from a voltage source and compresses the voltage pulse to generate a trigger signal. Compressing the voltage pulse includes increasing amplitude of the voltage pulse and decreasing length of the voltage pulse in time. An igniter receives the trigger signal and detonates an explosive responsive to receipt of the trigger signal.

  11. Acid Gas Capture Using CO2-Binding Organic Liquids

    SciTech Connect (OSTI)

    Heldebrant, David J.; Koech, Phillip K.; Rainbolt, James E.; Zheng, Feng

    2010-11-10T23:59:59.000Z

    Current chemical CO2 scrubbing technology is primarily aqueous alkanolamine based. These systems rapidly bind CO2 (forming water-soluble carbamate and bicarbonate salts) however, the process has serious disadvantages. The concentration of monoethanolamine rarely exceeds 30 wt % due to the corrosive nature of the solution, and this reduces the maximum CO2 volumetric (?108 g/L) and gravimetric capacity (?7 wt%) of the CO2 scrubber. The ?30 wt % loading of ethanolamine also means that a large excess of water must be pumped and heated during CO2 capture and release, and this greatly increases the energy requirements especially considering the high specific heat of water (4 j/g-1K-1). Our approach is to switch to organic systems that chemically bind CO2 as liquid alkylcarbonate salts. Our CO2-binding organic liquids have higher CO2 solubility, lower specific heats, potential for less corrosion and lower binding energies for CO2 than aqueous systems. CO2BOLs also reversibly bind and release mixed sulfur oxides. Furthermore the CO2BOL system can be direct solvent replacements for any solvent based CO2 capture systems because they are commercially available reagents and because they are fluids they would not require extensive process re-engineering.

  12. Dynamic Structural Rearrangements Between DNA Binding Modes of E. coli SSB Protein

    E-Print Network [OSTI]

    Lohman, Timothy M.

    an oligonucleotide/oligosaccharide bind- ing (OB) fold,5,7­9 hence the tetramer has four po- tential ssDNA binding sites. The SSB tetramer can bind long ssDNA in a variety of binding modes depending on solutionCl) and high protein to DNA ratios, an SSB tetramer binds to ssDNA with high inter-tetramer cooperativity using

  13. Accurate nuclear radii and binding energies from a chiral interaction

    E-Print Network [OSTI]

    Ekstrom, A; Wendt, K A; Hagen, G; Papenbrock, T; Carlsson, B D; Forssen, C; Hjorth-Jensen, M; Navratil, P; Nazarewicz, W

    2015-01-01T23:59:59.000Z

    The accurate reproduction of nuclear radii and binding energies is a long-standing challenge in nuclear theory. To address this problem two-nucleon and three-nucleon forces from chiral effective field theory are optimized simultaneously to low-energy nucleon-nucleon scattering data, as well as binding energies and radii of few-nucleon systems and selected isotopes of carbon and oxygen. Coupled-cluster calculations based on this interaction, named NNLOsat, yield accurate binding energies and radii of nuclei up to 40Ca, and are consistent with the empirical saturation point of symmetric nuclear matter. In addition, the low-lying collective 3- states in 16O and 40Ca are described accurately, while spectra for selected p- and sd-shell nuclei are in reasonable agreement with experiment.

  14. BIODIVERSITY Accounting for tree line shift, glacier

    E-Print Network [OSTI]

    Zimmermann, Niklaus E.

    BIODIVERSITY RESEARCH Accounting for tree line shift, glacier retreat and primary succession land cover (tree line shift, glacier retreat and primary succession) into species distribution model. Methods We fit linear mixed effects (LME) models to historical changes in forest and glacier cover

  15. LA NACION LINE Ciencia/Salud

    E-Print Network [OSTI]

    Wilf, Peter

    LA NACION LINE Ciencia/Salud Viernes 4 de Abril de 2003 LA NACION LINE | Ed. Anteriores | 4 de Abril de 2003 | Ciencia/Salud | Nota Estudio del Museo Egidio Feruglio, de Trelew La diversidad vegetal://www.lanacion.com.ar/03/04/04/sl_486019.asp LA NACION | 04/04/2003 | Página 14 | Ciencia/Salud Volver a ediciones

  16. Speed-line for 3D animation 

    E-Print Network [OSTI]

    Song, Won Chan

    2007-04-25T23:59:59.000Z

    My thesis describes a tool which creates speed-lines automatically in 3D computer animations. Speed-lines are usually used in comic books to express fast motions in a still image. They are also used in 2D animations. ...

  17. results and benefits... The Bittern Line Carbon

    E-Print Network [OSTI]

    Everest, Graham R

    results and benefits... The Bittern Line Carbon Neutral Stations Transport Regeneration Ltd. June 2008 c a s e s t u d yCRed carbon reduction Project Summary Our client, Transport Regeneration Ltd., aims to make nine stations on the Bittern Line between Norwich and Sheringham carbon neutral

  18. The Linux Command Line Second Internet Edition

    E-Print Network [OSTI]

    Dahl, David B.

    The Linux Command Line Second Internet Edition William E. Shotts, Jr. A LinuxCommand.org Book #12....................................................................................................xvi Why Use The Command Line to their respective owners. This book is part of the LinuxCommand.org project, a site for Linux education and advo

  19. On-line secret sharing Laszlo Csirmaz

    E-Print Network [OSTI]

    Tardos, Gábor

    On-line secret sharing L´aszl´o Csirmaz G´abor Tardos Abstract In a perfect secret sharing scheme the dealer distributes shares to participants so that qualified subsets can recover the secret, while unqualified subsets have no information on the secret. In an on-line secret sharing scheme the dealer assigns

  20. Optical on line techniques for nuclear applications

    SciTech Connect (OSTI)

    Doizi, D. [CEA, Nuclear Energy Div. DEN, DANS/DPC/SECR/LSRM (France); Pailloux, A.; Maury, C.; Sirven, J. B.; Dauvois, V.; Roujou, J. L.; Rosset, C. D. [CEA, Nuclear Energy Div. DEN, DANS/DPC (France); Hartmann, J. M. [CNRS., UMR 7583, LISA, Creteil (France)

    2011-07-01T23:59:59.000Z

    Optical on line techniques enable non intrusive physical measurements in harsh environments (high temperature, high pressure, radioactivity, ...). Optical absorption spectrometries such as UV-Visible, FTIR, CRDS have been successfully used to study gas phase speciation in different nuclear applications. LIBS which relies on laser matter interactions is a on line optical technique for solids and liquids elementary analysis. (authors)

  1. Spectroscopy of $^{28}$Na: shell evolution toward the drip line

    E-Print Network [OSTI]

    Lepailleur, A; Mutschler, A; Sorlin, O; Bader, V; Bancroft, C; Barofsky, D; Bastin, B; Baugher, T; Bazin, D; Bildstein, V; Borcea, C; Borcea, R; Brown, B A; Caceres, L; Gade, A; Gaudefroy, L; Grévy, S; Grinyer, G F; Iwasaki, H; Khan, E; Kröll, T; Langer, C; Lemasson, A; Llidoo, O; Lloyd, J; Negoita, F; Santos, F de Oliveira; Perdikakis, G; Recchia, F; Redpath, T; Roger, T; Rotaru, F; Saenz, S; Saint-Laurent, M -G; Smalley, D; Sohler, D; Stanoiu, M; Stroberg, S R; Thomas, J C; Vandebrouck, M; Weisshaar, D; Westerberg, A

    2015-01-01T23:59:59.000Z

    Excited states in $^{28}$Na have been studied using the $\\beta$-decay of implanted $^{28}$Ne ions at GANIL/LISE as well as the in-beam $\\gamma$-ray spectroscopy at the NSCL/S800 facility. New states of positive (J$^{\\pi}$=3,4$^+$) and negative (J$^{\\pi}$=1-5$^-$) parity are proposed. The former arise from the coupling between 0d$\\_{5/2}$ protons and a 0d$\\_{3/2}$ neutron, while the latter are due to couplings with 1p$\\_{3/2}$ or 0f$\\_{7/2}$ neutrons. While the relative energies between the J$^{\\pi}$=1-4$^+$ states are well reproduced with the USDA interaction in the N=17 isotones, a progressive shift in the ground state binding energy (by about 500 keV) is observed between $^{26}$F and $^{30}$Al. This points to a possible change in the proton-neutron 0d$\\_{5/2}$-0d$\\_{3/2}$ effective interaction when moving from stability to the drip line. The presence of J$^{\\pi}$=1-4$^-$ negative parity states around 1.5 MeV as well as of a candidate for a J$^{\\pi}$=5$^-$ state around 2.5 MeV give further support to the col...

  2. Design of the ILC RTML extraction lines

    SciTech Connect (OSTI)

    Seletskiy, S.; Tenenbaum, P.; Walz, D.; /SLAC; Solyak, N.; /Fermilab

    2008-06-01T23:59:59.000Z

    The ILC [1] Damping Ring to the Main Linac beamline (RTML) contains three extraction lines (EL). Each EL can be used both for an emergency abort dumping of the beam and tune-up continual train-by-train extraction. Two of the extraction lines are located downstream of the first and second stages of the RTML bunch compressor, and must accept both compressed and uncompressed beam with energy spreads of 2.5% and 0.15%, respectively. In this paper we report on an optics design that allowed minimizing the length of the extraction lines while offsetting the beam dumps from the main line by the distance required for acceptable radiation levels in the service tunnel. The proposed extraction lines can accommodate beams with different energy spreads while at the same time providing the beam size acceptable for the aluminum dump window.

  3. Product Lines that supply other Product Lines: A Service-Oriented Approach Salvador Trujillo

    E-Print Network [OSTI]

    Apel, Sven

    Product Lines that supply other Product Lines: A Service-Oriented Approach Salvador Trujillo@uni-passau.de Abstract A software product line is a family of software products that share a set of core assets with the goal of reuse. In this paper, we focus on a scenario in which different products from different product

  4. Calculating Routes A Long a Line Assumes that lines exist as a shapefile

    E-Print Network [OSTI]

    Brownstone, Rob

    Calculating Routes A Long a Line Assumes that lines exist as a shapefile Step 1 ­ Create a Route from the Line Shapefile Go to ArcToolBox ­ Linear Referencing Tools ­ Create Route. Fill in Fields;Step 4 ­ Convert to Shapefile Make the event file permanent. Right click on the event table

  5. Molecular Cell High-Affinity Binding of Chp1 Chromodomain

    E-Print Network [OSTI]

    Halazonetis, Thanos

    Molecular Cell Article High-Affinity Binding of Chp1 Chromodomain to K9 Methylated Histone H3, Chp1, and siRNAs derived from centro- meric repeats. Recruitment of RITS to centromeres has been establishment. Our crystal structure of Chp1's chromodomain in complex with a trimethylated lysine 9 H3 peptide

  6. Methods of detection using a cellulose binding domain fusion product

    DOE Patents [OSTI]

    Shoseyov, Oded (Shimshon, IL); Shpiegl, Itai (North Gallilea, IL); Goldstein, Marc A. (Davis, CA); Doi, Roy H. (Davis, CA)

    1999-01-01T23:59:59.000Z

    A cellulose binding domain (CBD) having a high affinity for crystalline cellulose and chitin is disclosed, along with methods for the molecular cloning and recombinant production thereof. Fusion products comprising the CBD and a second protein are likewise described. A wide range of applications are contemplated for both the CBD and the fusion products, including drug delivery, affinity separations, and diagnostic techniques.

  7. Workshop on gate valve pressure locking and thermal binding

    SciTech Connect (OSTI)

    Brown, E.J.

    1995-07-01T23:59:59.000Z

    The purpose of the Workshop on Gate Valve Pressure Locking and Thermal Binding was to discuss pressure locking and thermal binding issues that could lead to inoperable gate valves in both boiling water and pressurized water reactors. The goal was to foster exchange of information to develop the technical bases to understand the phenomena, identify the components that are susceptible, discuss actual events, discuss the safety significance, and illustrate known corrective actions that can prevent or limit the occurrence of pressure locking or thermal binding. The presentations were structured to cover U.S. Nuclear Regulatory Commission staff evaluation of operating experience and planned regulatory activity; industry discussions of specific events, including foreign experience, and efforts to determine causes and alleviate the affects; and valve vendor experience and recommended corrective action. The discussions indicated that identifying valves susceptible to pressure locking and thermal binding was a complex process involving knowledge of components, systems, and plant operations. The corrective action options are varied and straightforward.

  8. Experimental study of exiton binding energy in semiconducting carbon nanotubes

    E-Print Network [OSTI]

    Maruyama, Shigeo

    and Technology (AIST), Tsukuba 305-8565, Japan 3 Global Edge Institute, Tokyo Institute of Technology, Tokyo, Japan 4 Departement of Mechanical Engineering, The University of Tokyo, Tokyo, Japan (Dated: July 21 dimensional nanotube leads to strong electron-hole localiza- tion, with binding energy as high as 0.5 e

  9. Parkin Binds to / Tubulin and Increases their Ubiquitination and Degradation

    E-Print Network [OSTI]

    Feng, Jian

    Parkin Binds to / Tubulin and Increases their Ubiquitination and Degradation Yong Ren, Jinghui Zhao depolymerize microtubules and increase tubulin degradation. Microtu- bules are polymers of tubulin. Misfolded tubulin monomers are highly toxic and quickly degraded through a hitherto unknown mechanism. Here

  10. Methods of detection using a cellulose binding domain fusion product

    DOE Patents [OSTI]

    Shoseyov, O.; Shpiegl, I.; Goldstein, M.A.; Doi, R.H.

    1999-01-05T23:59:59.000Z

    A cellulose binding domain (CBD) having a high affinity for crystalline cellulose and chitin is disclosed, along with methods for the molecular cloning and recombinant production. Fusion products comprising the CBD and a second protein are likewise described. A wide range of applications are contemplated for both the CBD and the fusion products, including drug delivery, affinity separations, and diagnostic techniques. 34 figs.

  11. Methods of use of cellulose binding domain proteins

    DOE Patents [OSTI]

    Shoseyov, Oded (Karmey Yosef, IL); Shpiegl, Itai (Rehovot, IL); Goldstein, Marc A. (Davis, CA); Doi, Roy H. (Davis, CA)

    1997-01-01T23:59:59.000Z

    A cellulose binding domain (CBD) having a high affinity for crystalline cellulose and chitin is disclosed, along with methods for the molecular cloning and recombinant production thereof. Fusion products comprising the CBD and a second protein are likewise described. A wide range of applications are contemplated for both the CBD and the fusion products, including drug delivery, affinity separations, and diagnostic techniques.

  12. Knowledge and Obedience: The Developmental Status of the Binding Theory

    E-Print Network [OSTI]

    Grimshaw, Jane; Rosen, Sara Thomas

    1990-04-05T23:59:59.000Z

    counterpart of sentences like Johnt tM Bill to shave him,. Thi» binding relation is generally judged as ungrammatical, yet speakers preferred this interpretation to one in which the pronoun had no antecedent. K N O W I . h D O Ii A N D O B H D I H N C H 203...

  13. Thesis and Dissertation Archiving and Binding Request Form

    E-Print Network [OSTI]

    Bieber, Michael

    Thesis and Dissertation Archiving and Binding Request Form The Van Houten Library's digital copy is for archival purposes which will be accessible through NJIT's Electronic Theses & Dissertations website approved Master's Thesis or Doctoral Dissertation before it can be forwarded by the Graduate Studies Office

  14. Methods of use of cellulose binding domain proteins

    DOE Patents [OSTI]

    Shoseyov, O.; Shpiegl, I.; Goldstein, M.A.; Doi, R.H.

    1997-09-23T23:59:59.000Z

    A cellulose binding domain (CBD) having a high affinity for crystalline cellulose and chitin is disclosed, along with methods for the molecular cloning and recombinant production. Fusion products comprising the CBD and a second protein are likewise described. A wide range of applications are contemplated for both the CBD and the fusion products, including drug delivery, affinity separations, and diagnostic techniques. 16 figs.

  15. Off-Shell NN Potential and Triton Binding Energy

    E-Print Network [OSTI]

    Y. Song; R. Machleidt

    1994-03-31T23:59:59.000Z

    The NONLOCAL Bonn-B potential predicts 8.0 MeV binding energy for the triton (in a charge-dependent 34-channel Faddeev calculation) which is about 0.4 MeV more than the predictions by LOCAL NN potentials. We pin down origin and size of the nonlocality in the Bonn potential, in analytic and numeric form. The nonlocality is due to the use of the correct off-shell Feynman amplitude of one-boson-exchange avoiding the commonly used on-shell approximations which yield the local potentials. We also illustrate how this off-shell behavior leads to more binding energy. We emphasize that the increased binding energy is not due to on-shell differences (differences in the fit of the NN data or phase shifts). In particular, the Bonn-B potential reproduces accurately the $\\epsilon_1$ mixing parameter up to 350 MeV as determined in the recent Nijmegen multi-energy NN phase-shift analysis. Adding the relativistic effect from the relativistic nucleon propagators in the Faddeev equations, brings the Bonn-B result up to 8.2 MeV triton binding. This leaves a difference of only 0.3 MeV to experiment, which may possibly be explained by refinements in the treatment of relativity and the inclusion of other nonlocalities (e.~g., quark-gluon exchange at short range). Thus, it is conceivable that a realistic NN potential which describes the NN data up to 300 MeV correctly may explain the triton binding energy without recourse to 3-N forces; relativity would play a major role for this result.

  16. Grand Coulee Transmission Line Replacement Project

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    by the Bureau of Reclamation to design and construct six new 500-kV overhead transmission lines at Grand Coulee Dam. BPA will replace the existing underground transmission...

  17. Ames Lab 101: Next Generation Power Lines

    ScienceCinema (OSTI)

    Russell, Alan

    2012-08-29T23:59:59.000Z

    Ames Laboratory scientist Alan Russell discusses the need to develop new power lines that are stronger and more conductive as a way to address the problem of the nation's aging and inadequate power grid.

  18. DNA Assembly Line for Nano-Construction

    ScienceCinema (OSTI)

    Oleg Gang

    2010-01-08T23:59:59.000Z

    Building on the idea of using DNA to link up nanoparticles scientists at Brookhaven National Lab have designed a molecular assembly line for high-precision nano-construction. Nanofabrication is essential for exploiting the unique properties of nanoparticl

  19. Lining Over Refractory - Conserve Energy and Capital

    E-Print Network [OSTI]

    Jost, M. L.; Barrows, G. L.

    1980-01-01T23:59:59.000Z

    .~. LINING OVER REFRACTORY - CONSERVE ENERGY & CAPITAL by Mark L. Jost Gerald L. Barrows The Carborundum Company Niagara Falls, New York INTRODUCTION Companies operating industrial heating equip Advantages ment find themselves coming under...

  20. IIR algorithms for adaptive line enhancement

    SciTech Connect (OSTI)

    David, R.A.; Stearns, S.D.; Elliott, G.R.; Etter, D.M.

    1983-01-01T23:59:59.000Z

    We introduce a simple IIR structure for the adaptive line enhancer. Two algorithms based on gradient-search techniques are presented for adapting the structure. Results from experiments which utilized real data as well as computer simulations are provided.

  1. Line Environment, Safety and Health Oversight

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    1997-06-26T23:59:59.000Z

    Sets forth the Department's expectations line management environment, safety and health (ES&H) oversight and for the use of contractor self-assessment programs as the cornerstone for this oversight. Canceled by DOE O 226.1.

  2. Electrical Transmission Line Diametrical Retention Mechanism

    DOE Patents [OSTI]

    Hall, David R. (Provo, UT); Hall, Jr., H. Tracy (Provo, UT); Pixton, David (Lehi, UT); Dahlgren, Scott (Provo, UT); Sneddon, Cameron (Provo, UT); Briscoe, Michael (Lehi, UT); Fox, Joe (Spanish Fork, UT)

    2006-01-03T23:59:59.000Z

    The invention is a mechanism for retaining an electrical transmission line. In one embodiment of the invention it is a system for retaining an electrical transmission line within downhole components. The invention allows a transmission line to be attached to the internal diameter of drilling components that have a substantially uniform drilling diameter. In accordance with one aspect of the invention, the system includes a plurality of downhole components, such as sections of pipe in a drill string, drill collars, heavy weight drill pipe, and jars. The system also includes a coaxial cable running between the first and second end of a drill pipe, the coaxial cable having a conductive tube and a conductive core within it. The invention allows the electrical transmission line to withstand the tension and compression of drill pipe during routine drilling cycles.

  3. Temperatures in the blast furnace refractory lining

    SciTech Connect (OSTI)

    Hebel, R.; Streuber, C. [Didier-M and P Energietechnik GmbH, Wiesbaden (Germany); Steiger, R. [Didier-M and P Engineering Services, Highland, IN (United States); Jeschar, R. [TU Clausthal (Germany). Inst. fuer Energieverfahrenstechnik und Brennstofftechnik

    1995-12-01T23:59:59.000Z

    The campaign life duration of a blast furnace is mainly determined by the condition of the refractory lining in heavy-duty zones such as the hearth, bosh, belly and lower stack. To achieve a desired lifetime, the temperature of the lining in these areas thereby proved to be the decisive controllable parameter. Low operating temperatures result in prolonged service life and are attained through high cooling efficiency. Besides the refractory grade chosen, the wear profile is mainly determined by the type of cooling system applied and the cooling intensity. Therefore, an appropriate compromise between long service life and energy losses has to be found in each case. In order to predict the service life of a lining it is important to know the wear condition at all times during the campaign. The paper describes the approaches the authors have made so far on European blast furnaces, on a theoretical and practical basis, on how to analyze the lining wear.

  4. Gila to North Gila Transmission Line Rebuild

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    the new APS line. Western's land action entails acquiring up to an additional 50 feet right-of-way adjacent to the existing Gila Knob ROW, transferring a portion of the Gila...

  5. Heat-Traced Fluid Transfer Lines

    E-Print Network [OSTI]

    Schilling, R. E.

    1984-01-01T23:59:59.000Z

    HEAT-TRACED FLUID TRANSFER LINES Robert E. Schilling, P.E. Eaton Corporation Aurora, Ohio This paper discusses basic considerations in designing a heat tracing system using either steam or electrical tracing. Four basic reasons to heat...

  6. Productivity Improvement of a Manual Assembly Line

    E-Print Network [OSTI]

    Yerasi, Pranavi

    2012-10-19T23:59:59.000Z

    The current project addresses the productivity improvement of a manual assembly line by making use of operations analysis in the framework of Lean production. A methodology is proposed that helps to improve the productivity of any production process...

  7. Ames Lab 101: Next Generation Power Lines

    SciTech Connect (OSTI)

    Russell, Alan

    2010-01-01T23:59:59.000Z

    Ames Laboratory scientist Alan Russell discusses the need to develop new power lines that are stronger and more conductive as a way to address the problem of the nation's aging and inadequate power grid.

  8. What is the Broad Line Region?

    E-Print Network [OSTI]

    Ari Laor

    2003-12-16T23:59:59.000Z

    What is the Broad Line Region (BLR) made of? What determines its location? Why is it sometimes missing? What controls its properties? Some recent results and new approaches which may shed light on these issues are briefly described.

  9. Corona losses in HVdc bipolar lines

    SciTech Connect (OSTI)

    Corbellini, U.; Pelacchi, P. [Univ. of Pisa (Italy). Dept. of Electric Systems and Automation] [Univ. of Pisa (Italy). Dept. of Electric Systems and Automation

    1996-07-01T23:59:59.000Z

    The problem related to the prediction of corona losses in HVdc bipolar lines has been solved, in the past, by means of semi-empirical monomial formulae. However, the proposed formulae that are simpler to use do not always give adequate calculation precision, while the formulae that provide the closest results require implicit functions of different complexity, which are difficult to apply; moreover, it is not possible to understand clearly what influence the variations of the different line parameters have on the losses themselves. The new monomial semi-empirical relationship, proposed to predict the corona losses in HVdc bipolar lines, is very simple to use; it highlights the dependence of power losses due to the corona effect by the different line parameters. The formula has been developed by elaborating a considerable amount of available experimental data.

  10. Design of the ILC RTML Extraction Lines

    SciTech Connect (OSTI)

    Seletskiy, S.; Tenenbaum, P.; Walz, D.; /SLAC; Solyak, N.; /Fermilab

    2011-10-17T23:59:59.000Z

    The ILC [1] Damping Ring to the Main Linac beamline (RTML) contains three extraction lines (EL). Each EL can be used both for an emergency abort dumping of the beam and tune-up continual train-by-train extraction. Two of the extraction lines are located downstream of the first and second stages of the RTML bunch compressor, and must accept both compressed and uncompressed beam with energy spreads of 2.5% and 0.15%, respectively. In this paper we report on an optics design that allowed minimizing the length of the extraction lines while offsetting the beam dumps from the main line by the distance required for acceptable radiation levels in the service tunnel. The proposed extraction lines can accommodate beams with different energy spreads while at the same time providing the beam size acceptable for the aluminum dump window. The RTML incorporates three extraction lines, which can be used for either an emergency beam abort or for a train-by-train extraction. The first EL is located downstream of the Damping Ring extraction arc. The other two extraction lines are located downstream of each stage of the two-stage bunch compressor. The first extraction line (EL1) receives 5GeV beam with an 0.15% energy spread. The extraction line located downstream of the first stage of bunch compressor (ELBC1) receives both compressed and uncompressed beam, and therefore must accept beam with both 5 and 4.88GeV energy, and 0.15% and 2.5% energy spread, respectively. The extraction line located after the second stage of the bunch compressor (ELBC2) receives 15GeV beam with either 0.15 or 1.8% energy spread. Each of the three extraction lines is equipped with the 220kW aluminum ball dump, which corresponds to the power of the continuously dumped beam with 5GeV energy, i.e., the beam trains must be delivered to the ELBC2 dump at reduced repetition rate.

  11. DOI: 10.1002/cbic.200500285 Binding of Helix-Threading Peptides to E. coli

    E-Print Network [OSTI]

    Beal, Peter A.

    ] To further explore and develop the capabilities of the HTP design for binding RNA selectively, we identified duplex grooves. To further explore and develop the capabili- ties of the HTP design for binding RNA

  12. Glucose oxidation in heart-type fatty acid binding protein null mice 

    E-Print Network [OSTI]

    Adhikari, Sean

    2006-10-30T23:59:59.000Z

    Heart-type fatty acid binding protein (H-FABP) is a major fatty acid binding factor in skeletal muscles. Genetic lack of H-FABP severely impairs the esterification and oxidation of exogenous fatty acids in soleus muscles ...

  13. V-058: Microsoft Internet Explorer CDwnBindInfo Object Reuse...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    8: Microsoft Internet Explorer CDwnBindInfo Object Reuse Flaw Lets Remote Users Execute Arbitrary Code V-058: Microsoft Internet Explorer CDwnBindInfo Object Reuse Flaw Lets Remote...

  14. Characterization of Mg Binding to the DNA Repair ProteinApurinic...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Mg Binding to the DNA Repair Protein ApurinicApyrimidic Endonuclease 1 via Solid-State Mg NMR Spectroscopy. Characterization of Mg Binding to the DNA Repair Protein Apurinic...

  15. acid-binding protein genes: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The adipocyte fatty acid-binding protein aP2 regulates systemic glucose (more) Shum, Bennett Oh Vic 2007-01-01 2 Fatty acid-binding protein in bovine skeletal muscle Texas A&M...

  16. aberrant fatty acid-binding: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The adipocyte fatty acid-binding protein aP2 regulates systemic glucose (more) Shum, Bennett Oh Vic 2007-01-01 2 Fatty acid-binding protein in bovine skeletal muscle Texas A&M...

  17. acid-binding protein ii: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The adipocyte fatty acid-binding protein aP2 regulates systemic glucose (more) Shum, Bennett Oh Vic 2007-01-01 2 Fatty acid-binding protein in bovine skeletal muscle Texas A&M...

  18. acid-binding immunoglobulin-like lectin-6: Topics by E-print...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The adipocyte fatty acid-binding protein aP2 regulates systemic glucose (more) Shum, Bennett Oh Vic 2007-01-01 2 Fatty acid-binding protein in bovine skeletal muscle Texas A&M...

  19. Nucleon binding corrections to lepton-nucleus deep inelastic scattering: Use of a realistic spectral function

    SciTech Connect (OSTI)

    Dieperink, A.E.L.; Miller, G.A. (Department of Physics, FM-15, University of Washington, Seattle, Washington (USA))

    1991-08-01T23:59:59.000Z

    Nuclear spectral functions computed with realistic nuclear forces are used to compute mean separation energies and to estimate the binding corrections to lepton-nucleus deep inelastic scattering. The separation energies are large and significant binding effects are obtained.

  20. Discovery and Characterization of a Cell-Permeable, Small-Molecule c-Abl Kinase Activator that Binds to the Myristoyl Binding Site

    SciTech Connect (OSTI)

    Yang, Jingsong; Campobasso, Nino; Biju, Mangatt P.; Fisher, Kelly; Pan, Xiao-Qing; Cottom, Josh; Galbraith, Sarah; Ho, Thau; Zhang, Hong; Hong, Xuan; Ward, Paris; Hofmann, Glenn; Siegfried, Brett; Zappacosta, Francesca; Washio, Yoshiaki; Cao, Ping; Qu, Junya; Bertrand, Sophie; Wang, Da-Yuan; Head, Martha S.; Li, Hu; Moores, Sheri; Lai, Zhihong; Johanson, Kyung; Burton, George; Erickson-Miller, Connie; Simpson, Graham; Tummino, Peter; Copeland, Robert A.; Oliff, Allen (GSKPA)

    2014-10-02T23:59:59.000Z

    c-Abl kinase activity is regulated by a unique mechanism involving the formation of an autoinhibited conformation in which the N-terminal myristoyl group binds intramolecularly to the myristoyl binding site on the kinase domain and induces the bending of the {alpha}I helix that creates a docking surface for the SH2 domain. Here, we report a small-molecule c-Abl activator, DPH, that displays potent enzymatic and cellular activity in stimulating c-Abl activation. Structural analyses indicate that DPH binds to the myristoyl binding site and prevents the formation of the bent conformation of the {alpha}I helix through steric hindrance, a mode of action distinct from the previously identified allosteric c-Abl inhibitor, GNF-2, that also binds to the myristoyl binding site. DPH represents the first cell-permeable, small-molecule tool compound for c-Abl activation.

  1. Delivering Heparin-Binding Insulin-Like Growth Factor 1 with Self-Assembling Peptide Hydrogels

    E-Print Network [OSTI]

    Miller, Rachel E.

    Heparin-binding insulin-like growth factor 1 (HB-IGF-1) is a fusion protein of IGF-1 with the HB domain of heparin-binding epidermal growth factor-like growth factor. A single dose of HB-IGF-1 has been shown to bind ...

  2. Ultrastrong Optical Binding of Metallic Nanoparticles Vassili Demergis and Ernst-Ludwig Florin*

    E-Print Network [OSTI]

    Texas at Austin. University of

    Ultrastrong Optical Binding of Metallic Nanoparticles Vassili Demergis and Ernst-Ludwig Florin the optical binding force, which has been assumed to be weak compared to the optical gradient and scattering forces. We show that trapping by the optical binding force can be over 20 times stronger than

  3. DNA binding shifts the redox potential of the transcription factor SoxR

    E-Print Network [OSTI]

    Dietrich, Lars

    DNA binding shifts the redox potential of the transcription factor SoxR Alon A. Gorodetsky , Lars E-modified electrodes are used to probe the effects of binding to DNA on the redox potential of SoxR, a transcription in the absence of DNA. Using Redmond red as a covalently bound redox reporter affixed above the SoxR binding site

  4. Theory of Free Energy and Entropy in Noncovalent Binding Huan-Xiang Zhou*,

    E-Print Network [OSTI]

    Weston, Ken

    Theory of Free Energy and Entropy in Noncovalent Binding Huan-Xiang Zhou*, and Michael K. Gilson, Rockville, Maryland 20850 Received December 23, 2008 Contents 1. Introduction 4092 2. Free Energy, Partition.4. Solvation and a Temperature-Dependent Energy Function 4096 3. Binding Free Energy and Binding Constant 4096

  5. Efficient Evaluation of Binding Free Energy Using Continuum Electrostatics Danzhi Huang and Amedeo Caflisch*

    E-Print Network [OSTI]

    Caflisch, Amedeo

    Efficient Evaluation of Binding Free Energy Using Continuum Electrostatics Solvation Danzhi Huang of the absolute free energy of binding. A predictive accuracy of about 1.0 kcal/mol is obtained for 13 and 29 into proteins of known structure require fast and accurate methods for the evaluation of binding free energies.1

  6. Molecular dissection of the roles of nucleotide binding and hydrolysis in dynein's AAA domains

    E-Print Network [OSTI]

    Vale, Ronald D.

    Molecular dissection of the roles of nucleotide binding and hydrolysis in dynein's AAA domains (ATPase associated with various cellular activities) domains that are thought to bind nucleotide; the role of nucleotide binding and hydrolysis in each of these four AAA domains has constituted an important and unre

  7. Origin of the Variation of Exciton Binding Energy in Semiconductors Marc Dvorak,1

    E-Print Network [OSTI]

    Wu, Zhigang

    Origin of the Variation of Exciton Binding Energy in Semiconductors Marc Dvorak,1 Su-Huai Wei,2, and the exciton binding energy Eb in technologically important semiconductors varies from merely a few me between the electronic band structures and exciton binding energies in semiconductors, employing first

  8. MATHEMATICAL ANALYSIS OF CONDUCTING AND SUPERCONDUCTING TRANSMISSION LINES

    E-Print Network [OSTI]

    Ramdani, Karim - Institut de Mathématiques �lie Cartan, Université Henri Poincaré

    MATHEMATICAL ANALYSIS OF CONDUCTING AND SUPERCONDUCTING TRANSMISSION LINES ANNE-SOPHIE BONNET propagation in the microstrip transmission lines used in microelectronics. In the first part, the case of the perfectly conducting strip. Key words. superconducting transmission lines, waveguides, spectral analysis

  9. Improved value for the silicon free exciton binding energy

    SciTech Connect (OSTI)

    Green, Martin A., E-mail: m.green@unsw.edu.au [Australian Centre for Advanced Photovoltaics, School of Photovoltaic and Renewable Energy Engineering, University of New South Wales, Sydney, Australia 2052 (Australia)

    2013-11-15T23:59:59.000Z

    The free exciton binding energy is a key parameter in silicon material and device physics. In particular, it provides the necessary link between the energy threshold for valence to conduction band optical absorption and the bandgap determining electronic properties. The long accepted low temperature binding energy value of 14.7 ± 0.4 meV is reassessed taking advantage of developments subsequent to its original determination, leading to the conclusion that this value is definitely an underestimate. Using three largely independent experimental data sets, an improved low temperature value of 15.01 ± 0.06 meV is deduced, in good agreement with the most comprehensive theoretical calculations to date.

  10. A new phenomenological formula for ground state binding energies

    E-Print Network [OSTI]

    G. Gangopadhyay

    2010-07-09T23:59:59.000Z

    A phenomenological formula based on liquid drop model has been proposed for ground state binding energies of nuclei. The effect due to bunching of single particle levels has been incorporated through a term resembling the one-body Hamiltonian. The effect of n-p interaction has been included through a function of valence nucleons. A total of 50 parameters has been used in the present calculation. The r.m.s. deviation for the binding energy values for 2140 nuclei comes out to be 0.376 MeV, and that for 1091 alpha decay energies is 0.284 MeV. The correspondence with the conventional liquid drop model is discussed.

  11. Uranium Exerts Acute Toxicity by Binding to Pyrroloquinoline Quinone Cofactor

    SciTech Connect (OSTI)

    Michael R. VanEngelen; Robert I. Szilagyi; Robin Gerlach; Brady E. Lee; William A. Apel; Brent M. Peyton

    2011-02-01T23:59:59.000Z

    Uranium as an environmental contaminant has been shown to be toxic to eukaryotes and prokaryotes; however, no specific mechanisms of uranium toxicity have been proposed so far. Here a combination of in vivo, in vitro, and in silico studies are presented describing direct inhibition of pyrroloquinoline quinone (PQQ)-dependent growth and metabolism by uranyl cations. Electrospray-ionization mass spectroscopy, UV-vis optical spectroscopy, competitive Ca2+/uranyl binding studies, relevant crystal structures, and molecular modeling unequivocally indicate the preferred binding of uranyl simultaneously to the carboxyl oxygen, pyridine nitrogen, and quinone oxygen of the PQQ molecule. The observed toxicity patterns are consistent with the biotic ligand model of acute metal toxicity. In addition to the environmental implications, this work represents the first proposed molecular mechanism of uranium toxicity in bacteria, and has relevance for uranium toxicity in many living systems.

  12. Nuclear binding, correlations and the origin of EMC effect

    E-Print Network [OSTI]

    Omar Benhar; Ingo SIck

    2012-07-19T23:59:59.000Z

    Recent data for the slope of the EMC-ratio in the intermediate $x$-region for {\\em light} nuclei, with $3 \\leq A \\leq 12$, have the potential to shed new light on the origin of the EMC effect. Here we study the role of nuclear binding using the scaling variable ${\\tilde y}$, best suited to take into account this effect, and the understanding of the average nucleon removal energies, $\\bar{E}$, provided by state-of-the-art calculations based on nuclear many body theory. We find an excellent correlation between the new EMC data at $x \\sim 0.5$ and $\\bar{E}$ for nuclei with $A$ from 3 to $\\infty$, indicating that in this $x$ region binding is an important ingredient to explain the EMC effect. The role played by nucleon-nucleon correlations in this context is also discussed.

  13. Nuclear binding, correlations and the origin of EMC effect

    E-Print Network [OSTI]

    Benhar, Omar

    2012-01-01T23:59:59.000Z

    Recent data for the slope of the EMC-ratio in the intermediate $x$-region for {\\em light} nuclei, with $3 \\leq A \\leq 12$, have the potential to shed new light on the origin of the EMC effect. Here we study the role of nuclear binding using the scaling variable ${\\tilde y}$, best suited to take into account this effect, and the understanding of the average nucleon removal energies, $\\bar{E}$, provided by state-of-the-art calculations based on nuclear many body theory. We find an excellent correlation between the new EMC data at $x \\sim 0.5$ and $\\bar{E}$ for nuclei with $A$ from 3 to $\\infty$, indicating that in this $x$ region binding is an important ingredient to explain the EMC effect. The role played by nucleon-nucleon correlations in this context is also discussed.

  14. On-site rubber lining -- Application of precured and self-curing rubber linings

    SciTech Connect (OSTI)

    Fenner, J. [Keramchemie GmbH, Siershahn (Germany)

    1998-12-31T23:59:59.000Z

    Rubber linings are applied as a corrosion protection system in a wide range of plants and installations of the industrial sector. In addition to the execution of lining works in a workshop, the on-site application of this corrosion protection system on the construction site has gained increasing importance. The various procedures utilized to apply the corrosion protection linings will be briefly presented in the following paper. In particular the precured and self curing rubber linings together with their scope of use and application on construction sites will be described in more detail.

  15. New Construction of Distribution Lines, Service Lines, and Appurtenant Facilities in Residential Subdivisions (New York)

    Broader source: Energy.gov [DOE]

    Any proposed construction of electricity-related facilities in residential subdivisions, including distribution and service lines and appurtenant facilities, is subject to these regulations, which...

  16. O.A.R. 734-055 - Pole Lines, Buried Cables, Pipe lines, Signs...

    Open Energy Info (EERE)

    buried cables, pipe lines, signs miscellaneous operations upon state highway right-of-way and properties under the jurisdiction of the department of transportation. Published N...

  17. ATM Networking in Linux Bindings occur at four distinct times

    E-Print Network [OSTI]

    Westall, James M.

    the following call: sock_register(pvc_proto_ops.family, &pvc_proto_ops); Family is PF_ATMPVC (as in PF_INET) pvc_len); : For ATM PVCs the structure is filled in as follows: static struct proto_ops pvc_proto_ops = { PF_ATMPVC, atm_create, pvc_dup, atm_release, pvc_bind, pvc_connect, : · The entry point addresses

  18. ATM Networking in Linux Bindings occur at four distinct times

    E-Print Network [OSTI]

    Westall, James M.

    using the following call: sock_register(pvc_proto_ops.family, &pvc_proto_ops); Family is PF_ATMPVC (as in PF_INET) pvc_proto_ops is a table of entry point addresses: struct proto_ops { int family; int_ops pvc_proto_ops = { PF_ATMPVC, atm_create, pvc_dup, atm_release, pvc_bind, pvc_connect, : . The entry

  19. Novel binding mechanism for ultra-long range molecules

    E-Print Network [OSTI]

    V. Bendkowsky; B. Butscher; J. Nipper; J. P. Shaffer; R. Loew; T. Pfau

    2008-09-17T23:59:59.000Z

    Molecular bonds can be divided into four primary types: ionic, covalent, van der Waals and hydrogen bonds. At ultralow temperatures a novel binding type emerges paving the way for novel molecules and ultracold chemistry [1,2]. The underlying mechanism for this new type of chemical bond is low-energy electron scattering of Rydberg electrons from polarisable ground state atoms [3]. This quantum scattering process can generate an attractive potential that is able to bind the ground state atom to the Rydberg atom at a well localized position within the Rydberg electron wave function. The resulting giant molecules can have an internuclear separation of several thousand Bohr radii, which places them among the largest known molecules to date. Their binding energies are much smaller than the Kepler frequencies of the Rydberg electrons i.e. the atomic Rydberg electron state is essentially unchanged by the bound ground state atom. Ultracold and dense samples of atoms enable the creation of these molecules via Rydberg excitation. In this paper we present spectroscopic evidence for the vibrational ground and first excited state of a Rubidium dimer Rb(5S)-Rb(nS). We apply a Born-Oppenheimer model to explain the measured binding energies for principal quantum numbers n between 34 and 40 and extract the s-wave scattering length for electron-Rb(5S) scattering in the relevant low energy regime Ekin < 100 meV. We also determine the lifetimes and the polarisabilities of these molecules. P-wave bound states [2], Trimer states [4] as well as bound states for large angular momentum of the Rydberg electron - socalled trilobite molecules [1] - are within reach in the near future and will further refine our conceptual understanding of the chemical bond.

  20. High molecular weight polysaccharide that binds and inhibits virus

    DOE Patents [OSTI]

    Konowalchuk, Thomas W

    2014-01-14T23:59:59.000Z

    This invention provides a high molecular weight polysaccharide capable of binding to and inhibiting virus and related pharmaceutical formulations and methods on inhibiting viral infectivity and/or pathogenicity, as well as immunogenic compositions. The invention further methods of inhibiting the growth of cancer cells and of ameliorating a symptom of aging. Additionally, the invention provides methods of detecting and/or quantifying and/or isolating viruses.

  1. PHYSICAL REVIEW B VOLUME 38, NUMBER 2 15 JULY 1988-I Density of states between Landau levels in a two-dimensional electron gas

    E-Print Network [OSTI]

    Glyde, Henry R.

    T (Ref. 11) and the SCBA short-range in- teraction theory (dashed line). At B =5 T, fico, =8.7 me- turbative approach, the self-consistent Born approxima- tion (SCBA} which is siinilar to a coherent

  2. Supporting Information "Comparison of photoluminescence properties of semiconductor quantum dots and non-

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    microscope. The Hanburry Brown an Twiss time correlation system is depicted in the black dashed-line box, Andor Technology, Ireland). The Hanbury Brown and Twiss time-intensity correlation setup consist in two

  3. Carbon isotope variability across the Ediacaran Yangtze platform in South China: Implications for a large surface-to-deep

    E-Print Network [OSTI]

    Jiang, Ganqing

    Carbon isotope variability across the Ediacaran Yangtze platform in South China: Implications Formation; Dengying Formation; Yangtze platform; South China 1. Introduction Recent carbon isotope studies of South China and position of the Ediacaran carbonate platform margin (coarse dashed line). (B

  4. LS-35 6 GeV Light Source Storage Ring Quadrupole and Sextupole...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    1 shows the cross section of one-fourth of a storage ring quadru- pole magnet. The vacuum chamber outline is shown by the dashed line. A tapered pole is necessary to reduce the...

  5. SUPPLEMENTARY MATERIALS Exploring the vertical profile of atmospheric organic aerosol: comparing 17 aircraft field campaigns

    E-Print Network [OSTI]

    Meskhidze, Nicholas

    values. #12;Figure S2: Scatter plot of observed acetonitrile and OA concentrations for seven campaigns in black. 80th percentile acetonitrile concentrations for each campaign are indicated with a dashed line

  6. EIS-0483: Estes to Flatiron Substation Transmission Lines Rebuild...

    Broader source: Energy.gov (indexed) [DOE]

    EIS-0483: Estes to Flatiron Substation Transmission Lines Rebuild Project, Larimer County, Colorado EIS-0483: Estes to Flatiron Substation Transmission Lines Rebuild Project,...

  7. Request for Information on Permitting of Transmission Lines:...

    Energy Savers [EERE]

    Request for Information on Permitting of Transmission Lines: Federal Register Notice Volume 77, No. 38 - Feb. 27, 2012 Request for Information on Permitting of Transmission Lines:...

  8. EIS-0447: Champlain Hudson Power Express Transmission Line Project...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    47: Champlain Hudson Power Express Transmission Line Project, New York EIS-0447: Champlain Hudson Power Express Transmission Line Project, New York Summary This EIS evaluated the...

  9. EA-1880: Big Bend to Witten Transmission Line Project, South...

    Office of Environmental Management (EM)

    880: Big Bend to Witten Transmission Line Project, South Dakota EA-1880: Big Bend to Witten Transmission Line Project, South Dakota Summary The USDA Rural Utilities Service, with...

  10. Transfer Lines to Connect Liquid Waste Facilities and Salt Waste...

    Office of Environmental Management (EM)

    Transfer Lines to Connect Liquid Waste Facilities and Salt Waste Processing Facility Transfer Lines to Connect Liquid Waste Facilities and Salt Waste Processing Facility October...

  11. Glosten Associates Tests Innovative High-Strength Mooring Lines...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Glosten Associates Tests Innovative High-Strength Mooring Lines for Deepwater Offshore Wind Installations Glosten Associates Tests Innovative High-Strength Mooring Lines for...

  12. alpha line profile: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    calculations of IP line profiles based on hot model distributions of interplanetary hydrogen. We also find that the line profiles get narrower during solar maximum. The results...

  13. Spectroscopy of $^{28}$Na: shell evolution toward the drip line

    E-Print Network [OSTI]

    A. Lepailleur; K. Wimmer; A. Mutschler; O. Sorlin; V. Bader; C. Bancroft; D. Barofsky; B. Bastin; T. Baugher; D. Bazin; V. Bildstein; C. Borcea; R. Borcea; B. A. Brown; L. Caceres; A. Gade; L. Gaudefroy; S. Grévy; G. F. Grinyer; H. Iwasaki; E. Khan; T. Kröll; C. Langer; A. Lemasson; O. Llidoo; J. Lloyd; F. Negoita; F. de Oliveira Santos; G. Perdikakis; F. Recchia; T. Redpath; T. Roger; F. Rotaru; S. Saenz; M. -G. Saint-Laurent; D. Smalley; D. Sohler; M. Stanoiu; S. R. Stroberg; J. C. Thomas; M. Vandebrouck; D. Weisshaar; A. Westerberg

    2015-03-30T23:59:59.000Z

    Excited states in $^{28}$Na have been studied using the $\\beta$-decay of implanted $^{28}$Ne ions at GANIL/LISE as well as the in-beam $\\gamma$-ray spectroscopy at the NSCL/S800 facility. New states of positive (J$^{\\pi}$=3,4$^+$) and negative (J$^{\\pi}$=1-5$^-$) parity are proposed. The former arise from the coupling between 0d$\\_{5/2}$ protons and a 0d$\\_{3/2}$ neutron, while the latter are due to couplings with 1p$\\_{3/2}$ or 0f$\\_{7/2}$ neutrons. While the relative energies between the J$^{\\pi}$=1-4$^+$ states are well reproduced with the USDA interaction in the N=17 isotones, a progressive shift in the ground state binding energy (by about 500 keV) is observed between $^{26}$F and $^{30}$Al. This points to a possible change in the proton-neutron 0d$\\_{5/2}$-0d$\\_{3/2}$ effective interaction when moving from stability to the drip line. The presence of J$^{\\pi}$=1-4$^-$ negative parity states around 1.5 MeV as well as of a candidate for a J$^{\\pi}$=5$^-$ state around 2.5 MeV give further support to the collapse of the N=20 gap and to the inversion between the 0f$\\_{7/2}$ and 1p$\\_{3/2}$ levels below Z=12. These features are discussed in the framework of Shell Model and EDF calculations, leading to predicted negative parity states in the low energy spectra of the $^{26}$F and $^{25}$O nuclei.

  14. Deciphering the Effect of Nemaline-Myopathy Nebulin Mutations on Desmin Binding

    E-Print Network [OSTI]

    Jacobs, Krystyna M

    2012-07-11T23:59:59.000Z

    that desmin?s coil 2b binds as well to WT nebulin M160-164 to L5646A nebulin M160-164. ELISA assays were used to determine and compare the binding affinities (Kd) for the each of the nebulin fragments. Through ELISA assays we determined that the binding... nebulin M160-164 protein. The affinity constants (Kds) determined in experiment shown in Figure 6 are 10-fold different between the two samples. In particular, the lower Kd obtained for coil 2B binding to WT nebulin M160-164 suggests a stronger binding...

  15. Dynamic Line Rating: Research and Policy Evaluation

    SciTech Connect (OSTI)

    Jake P. Gentle; Kurt S. Myers; Michael R. West

    2014-07-01T23:59:59.000Z

    Dynamic Line Rating (DLR) is a smart grid technology that allows the rating of electrical conductors to be increased based on local weather conditions. Overhead lines are conventionally given a conservative rating based on worst case scenarios. We demonstrate that observing the conditions in real time leads to additional capacity and safer operation. This paper provides a report of a pioneering scheme in the United States of America in which DLR has been applied. Thereby, we demonstrate that observing the local weather conditions in real time leads to additional capacity and safer operation. Secondly, we discuss limitations involved. In doing so, we arrive at novel insights which will inform and improve future DLR projects. Third, we provide a policy background and discussion to clarify the technology’s potential and identifies barriers to the imminent adoption of dynamic line rating systems. We provide suggestions for regulatory bodies about possible improvements in policy to encourage adoption of this beneficial technology.

  16. Masking line foregrounds in intensity mapping surveys

    E-Print Network [OSTI]

    Breysse, Patrick C; Kamionkowski, Marc

    2015-01-01T23:59:59.000Z

    We address the problem of line confusion in intensity mapping surveys and explore the possibility to mitigate line foreground contamination by progressively masking the brightest pixels in the observed map. We consider experiments targeting CO(1-0) at $z=3$, Ly$\\alpha$ at $z=7$, and CII at $z=7$, and use simulated intensity maps, which include both clustering and shot noise components of the signal and possible foregrounds, in order to test the efficiency of our method. We find that for CO and Ly$\\alpha$ it is quite possible to remove most of the foreground contribution from the maps via only 1%-3% pixel masking. The CII maps will be more difficult to clean, however, due to instrumental constraints and the high-intensity foreground contamination involved. While the masking procedure sacrifices much of the astrophysical information present in our maps, we demonstrate that useful cosmological information in the targeted lines can be successfully retrieved.

  17. Critical line of the deconfinement phase transition

    E-Print Network [OSTI]

    M. I. Gorenstein; M. Gazdzicki; W. Greiner

    2005-05-17T23:59:59.000Z

    Phase diagram of strongly interacting matter is discussed within the exactly solvable statistical model of the quark-gluon bags. The model predicts two phases of matter: the hadron gas at a low temperature T and baryonic chemical potential muB, and the quark-gluon gas at a high T and/or muB. The nature of the phase transition depends on a form of the bag mass-volume spectrum (its pre-exponential factor), which is expected to change with the muB/T ratio. It is therefore likely that the line of the 1st} order transition at a high muB/T ratio is followed by the line of the 2nd order phase transition at an intermediate muB/T, and then by the lines of "higher order transitions" at a low muB/T.

  18. Load-resistant coaxial transmission line

    DOE Patents [OSTI]

    Hall, David R.; Fox, Joe

    2006-01-03T23:59:59.000Z

    A transmission line for downhole tools that make up all or part of a tool string for drilling and production of oil, gas, and geothermal wells that can withstand the dynamic gravitational forces and other accelerations associated with downhole excavations. The transmission line has a metal tube, or outer conductor, that houses a coaxial wire inner conductor. A non-metallic dielectric material is interposed between the inner and outer conductors. The outer and inner conductors and the dielectric are sufficiently compressed together so that independent motion between them is abated. Compression of the components of the transmission line may be achieved by drawing the transmission through one or more dies in order to draw down the outer conductor onto the dielectric, or by expanding the inner conductor against the dielectric using a mandrel or hydraulic pressure. Non-metallic bead segments may be used in aid of the compression necessary to resist the dynamic forces and accelerations of drilling.

  19. NF-{kappa}B suppresses HIF-1{alpha} response by competing for P300 binding

    SciTech Connect (OSTI)

    Mendonca, Daniela B.S., E-mail: daniela_mendonca@dentistry.unc.edu [Universidade Catolica de Brasilia, Pos-Graduacao em Ciencias Genomicas e Biotecnologia, SGAN Quadra 916, Av. W5 Norte, 70790-160 Brasilia, DF (Brazil); Bone Biology and Implant Therapy Laboratory, Department of Prosthodontics, University of North Carolina at Chapel Hill, 330 Brauer Hall, CB 7450, Chapel Hill, NC 27599 (United States); Mendonca, Gustavo [Universidade Catolica de Brasilia, Pos-Graduacao em Ciencias Genomicas e Biotecnologia, SGAN Quadra 916, Av. W5 Norte, 70790-160 Brasilia, DF (Brazil) [Universidade Catolica de Brasilia, Pos-Graduacao em Ciencias Genomicas e Biotecnologia, SGAN Quadra 916, Av. W5 Norte, 70790-160 Brasilia, DF (Brazil); Bone Biology and Implant Therapy Laboratory, Department of Prosthodontics, University of North Carolina at Chapel Hill, 330 Brauer Hall, CB 7450, Chapel Hill, NC 27599 (United States); Aragao, Francisco J.L. [Universidade Catolica de Brasilia, Pos-Graduacao em Ciencias Genomicas e Biotecnologia, SGAN Quadra 916, Av. W5 Norte, 70790-160 Brasilia, DF (Brazil) [Universidade Catolica de Brasilia, Pos-Graduacao em Ciencias Genomicas e Biotecnologia, SGAN Quadra 916, Av. W5 Norte, 70790-160 Brasilia, DF (Brazil); Embrapa Recursos Geneticos e Biotecnologia, Laboratorio de Introducao e Expressao de Genes, PqEB W5 Norte, 70770-900 Brasilia, DF (Brazil); Cooper, Lyndon F., E-mail: lyndon_cooper@dentistry.unc.edu [Bone Biology and Implant Therapy Laboratory, Department of Prosthodontics, University of North Carolina at Chapel Hill, 330 Brauer Hall, CB 7450, Chapel Hill, NC 27599 (United States)

    2011-01-28T23:59:59.000Z

    Research highlights: {yields} p65 completely blocked HIF-1{alpha} activity at the HRE on different cell lines. {yields} p65 caused minor changes in HIF-1{alpha} and HIF-1{alpha} target genes mRNA expression. {yields} p65 reduced transcription of VEGF promoter. {yields} p65 competes with HIF-1{alpha} for p300. -- Abstract: Hypoxia has emerged as a key determinant of osteogenesis. HIF-1{alpha} is the transcription factor mediating hypoxia responses that include induction of VEGF and related bone induction. Inflammatory signals antagonize bone repair via the NF-{kappa}B pathway. The present investigation explored the functional relationship of hypoxia (HIF-1{alpha} function) and inflammatory signaling (NF-{kappa}B) in stem like and osteoprogenitor cell lines. The potential interaction between HIF-1{alpha} and NF-{kappa}B signaling was explored by co-transfection studies in hFOB with p65, HIF-1{alpha} and 9x-HRE-luc or HIF-1{alpha} target genes reporter plasmids. Nuclear cross-talk was directly tested using the mammalian Gal4/VP16 two-hybrid, and confirmed by co-immunoprecipitation/western blotting assays. The results show that inflammatory stimulation (TNF-{alpha} treatment) causes a marked inhibition of HIF-1{alpha} function at the HRE in all cell lines studied. Also, co-transfection with p65 expression vector leads to reduced hVEGFp transcription after DFO-induced hypoxia. However, TNF-{alpha} treatment had little effect on HIF-1{alpha} mRNA levels. The functional interaction of Gal4-HIF-1{alpha} and VP16-p300 fusion proteins is effectively blocked by expression of p65 in a dose dependent manner. It was concluded that NF-{kappa}B-mediated inflammatory signaling is able to block HIF-1{alpha} transactivation at HRE-encoding genes by direct competition for p300 binding at the promoter. Inflammation may influence the stem cell niche and tissue regeneration by influencing cellular responses to hypoxia.

  20. Relativistic emission lines from accreting black holes - The effect of disk truncation on line profiles

    E-Print Network [OSTI]

    Andreas Mueller; Max Camenzind

    2003-09-30T23:59:59.000Z

    Relativistic emission lines generated by thin accretion disks around rotating black holes are an important diagnostic tool for testing gravity near the horizon. The iron K-line is of special importance for the interpretation of the X-ray emission of Seyfert galaxies, quasars and galactic X-ray binary systems. A generalized kinematic model is presented which includes radial drifts and non-Keplerian rotations for the line emitters. The resulting line profiles are obtained with an object-oriented ray tracer operating in the curved Kerr background metric. The general form of the Doppler factor is presented which includes all kinds of poloidal and toroidal motions near the horizon. The parameters of the model include the spin parameter, the inclination, the truncation and outer radius of the disk, velocity profiles for rotation and radial drift, the emissivity profile and a multi-species line-system. The red wing flux is generally reduced when radial drift is included as compared to the pure Keplerian velocity field. All resulting emission line profiles can be classified as triangular, double-horned, double-peaked, bumpy and shoulder-like. Of particular interest are emission line profiles generated by truncated standard accretion disks (TSD). It is also shown that the emissivity law has a great influence on the profiles. The characteristic shoulder-like line profile observed for the Seyfert galaxy MCG-6-30-15 can be reproduced for suitable parameters

  1. Abstract--Power Line Communications (PLC) is used for information exchange over the lines

    E-Print Network [OSTI]

    Boyer, Edmond

    Abstract-- Power Line Communications (PLC) is used for information exchange over the lines installed for delivering the electrical power. Inhome PLC is a technology which delivers telecom services to every corner of a household through already existing electrical wiring. In recent years, PLC has emerged

  2. Pion yield studies for proton drive beams of 2-8 GeV kinetic energy for stopped

    E-Print Network [OSTI]

    McDonald, Kirk

    data. Pion kinetic energy of 40 MeV corresponds to momentum of 113 MeV/c #12;MARS - dash-dotted lines #12;MARS - dash-dotted lines #12;#12;#12;HARP collaboration conclusion #12;#12;HARP vs HARP-CDP #12 angle pion production · Negative pion yield was studied at 10 GeV/c using JINR 2-m propane bubble

  3. New Construction of Distribution Lines, Service Lines, and Appurtenant Facilities in Certain Visually Significant Resources Outside Residential Subdivisions (New York)

    Broader source: Energy.gov [DOE]

    Any proposed construction of distribution lines, service lines, and appurtenant facilities to electric utilities located near scenic areas of statewide significance, including Adirondack park...

  4. An analysis of multiple component mooring lines

    E-Print Network [OSTI]

    Walters, Thomas Robert

    1977-01-01T23:59:59.000Z

    -5 Tension At Excursion Parameter Response--Weight Parameter 0. 2; Pretension Parameter 16 38 6-6 Line Length Parameter Response-- Weight Parameter 0. 2; Pretension Parameter 16 39 6-7 Excursion Parameter Response-- Weight Parameter 0. 2; Pretension... Parameter 2 40 6-8 Anchor Load Parameter Response-- Weight Parameter 0. 2; Pretension Parameter 2 41 6-9 Tension At Excursion Parameter Response--Weight Parameter 0. 2; Pretension Parameter 2 6-'I 0 Line Length Parameter Response-- Weight Parameter 0...

  5. Boron-10 Lined Proportional Counter Wall Effects

    SciTech Connect (OSTI)

    Siciliano, Edward R.; Kouzes, Richard T.

    2012-05-01T23:59:59.000Z

    The Department of Energy Office of Nuclear Safeguards (NA-241) is supporting the project 'Coincidence Counting With Boron-Based Alternative Neutron Detection Technology' at Pacific Northwest National Laboratory (PNNL) for development of an alternative neutron coincidence counter. The goal of this project is to design, build and demonstrate a boron-lined proportional tube based system in the configuration of a coincidence counter. This report provides information about how variations in proportional counter radius and gas pressure in a typical coincident counter design might affect the observed signal from boron-lined tubes. A discussion comparing tubes to parallel plate counters is also included.

  6. Inductive gas line for pulsed lasers

    DOE Patents [OSTI]

    Benett, William J. (Livermore, CA); Alger, Terry W. (Tracy, CA)

    1985-01-01T23:59:59.000Z

    A gas laser having a metal inlet gas feed line assembly shaped as a coil, to function as an electrical inductance and therefore high impedance to pulses of electric current applied to electrodes at opposite ends of a discharge tube of a laser, for example. This eliminates a discharge path for the laser through the inlet gas feed line. A ferrite core extends through the coil to increase the inductance of the coil and provide better electric isolation. By elimination of any discharge breakdown through the gas supply, efficiency is increased and a significantly longer operating lifetime of the laser is provided.

  7. Inductive gas line for pulsed lasers

    DOE Patents [OSTI]

    Benett, W.J.; Alger, T.W.

    1982-09-29T23:59:59.000Z

    A gas laser having a metal inlet gas feed line assembly shaped as a coil, to function as an electrical inductance and therefore high impedance to pulses of electric current applied to electrodes at opposite ends of a discharge tube of a laser, for example. This eliminates a discharge path for the laser through the inlet gas feed line. A ferrite core extends through the coil to increase the inductance of the coil and provide better electric isolation. By elimination of any discharge breakdown through the gas supply, efficiency is increased and a significantly longer operating lifetime of the laser is provided.

  8. Experiences with On-Line STEAMPOP

    E-Print Network [OSTI]

    Nath, R.; Libby, D. J.; Marcinkowska, A. R.; Skarke, S. C.; Thomasson, M. J.; Worsham, B. A.

    SUBSYSTEM Several types of fuel are usually available in the plant (Figure 3). For each gaseous fuel, there may be headers at various pressure levels. The external supplies of fuel feed into some of these headers. There are also PRVs connecting some... optimization, and extended ENERGYPOP for joint optimization of a process unit with the plant utility system [4 J ? All of these optimizers are off-line programs. Our long-term goal is to have the plant wide optimizer running in an on-line mode so...

  9. Concurrent Wind Cooling in Power Transmission Lines

    SciTech Connect (OSTI)

    Jake P Gentle

    2012-08-01T23:59:59.000Z

    Idaho National Laboratory and the Idaho Power Company, with collaboration from Idaho State University, have been working on a project to monitor wind and other environmental data parameters along certain electrical transmission corridors. The combination of both real-time historical weather and environmental data is being used to model, validate, and recommend possibilities for dynamic operations of the transmission lines for power and energy carrying capacity. The planned results can also be used to influence decisions about proposed design criteria for or upgrades to certain sections of the transmission lines.

  10. Ionization Chambers in the FLASH Dump Line

    E-Print Network [OSTI]

    . 7, 2010FLASH Seminar, Dec. 7, 2010 BPM 13DUMP Dump Line Upgrade 2009Dump Line Upgrade 2009 BPM 9DUMP BPM 5DUMP Toroid 9DUMP OTR screen 9DUMP BLM 14DUMP BLM 13.1DUMP 13.2DUMP BLM 9DUMP BLM 6DUMP BLM 1.1DUMP 1.2DUMP BPM 10DUMP BPM 16DUMP 8 x BHM 16DUMP BLM 14R.DUMP 14L.DUMP 14U.DUMP 14D.DUMP Ionization

  11. EC Transmission Line Risk Identification and Analysis

    SciTech Connect (OSTI)

    Bigelow, Tim S [ORNL

    2012-04-01T23:59:59.000Z

    The purpose of this document is to assist in evaluating and planning for the cost, schedule, and technical project risks associated with the delivery and operation of the EC (Electron cyclotron) transmission line system. In general, the major risks that are anticipated to be encountered during the project delivery phase associated with the implementation of the Procurement Arrangement for the EC transmission line system are associated with: (1) Undefined or changing requirements (e.g., functional or regulatory requirements) (2) Underperformance of prototype, first unit, or production components during testing (3) Unavailability of qualified vendors for critical components Technical risks associated with the design and operation of the system are also identified.

  12. Line formation in AGB atmospheres including velocity effects. Molecular line profile variations of long period variables

    E-Print Network [OSTI]

    Nowotny, W; Aringer, B

    2010-01-01T23:59:59.000Z

    The atmospheres of evolved red giants are considerably influenced by pulsations of the stellar interiors and developing stellar winds. The resulting complex velocity fields severely affect molecular line profiles observable in NIR spectra. With the help of model calculations the complex line formation process in AGB atmospheres was explored with the focus on velocity effects. Furthermore, we aimed for atmospheric models which are able to quantitatively reproduce line profile variations found in observed spectra of pulsating late-type giants. Models describing pulsation-enhanced dust-driven winds were used to compute synthetic spectra under the assumptions of chemical equilibrium and LTE and by solving the radiative transfer in spherical geometry including velocity effects. Radial velocities derived from Doppler-shifted synthetic line profiles provide information on the gas velocities in the line-forming region of the spectral features. On the basis of dynamic models we investigated in detail the finding that ...

  13. Comparing the performace of f1 testers versus their inbred line parents in evaluating experimental sorghum b and r lines in testcrosses.

    E-Print Network [OSTI]

    Packer, Daniel Jacob

    2009-05-15T23:59:59.000Z

    An appropriate tester correctly identifies the relative performance of experimental lines while maximizing the differences between lines. Most sorghum breeding programs use elite inbred lines testers. Inbred line testers evaluate experimental lines...

  14. Mechanism of Ubiquinol Oxidation by the bc1 Complex: Different Domains of the Quinol Binding Pocket and Their Role in the Mechanism and Binding of Inhibitors

    E-Print Network [OSTI]

    Crofts, Antony R.

    electron-transfer systems, occurring ubiquitously in respiratory and photosynthetic chains of mitochondriaMechanism of Ubiquinol Oxidation by the bc1 Complex: Different Domains of the Quinol Binding Pocket and Their Role in the Mechanism and Binding of Inhibitors Antony R. Crofts,*, Blanca Barquera,§ Robert B. Gennis

  15. Geomembranes for Canal Lining Geosynthetics 2009

    E-Print Network [OSTI]

    for this application including PVC, HDPE, LDPE, CSPE, and EPDM geomembranes. Frequently these geomembranes require for canal liners based on field test programs. The first such test program was started with a PVC test to evaluate the effectiveness of a range of canal lining alternatives. Traditionally, PVC geomembranes have

  16. Community Trees Power Lines and Trees

    E-Print Network [OSTI]

    Koford, Rolf R.

    aesthetic and psychological benefits. Figure 2. Grown into a power pole. #12;become hazardous to peopleCommunity Trees Power Lines and Trees Introduction Trees serve many purposes in urban and rural, hundreds of people throughout the United States are injured or killed each year when they climb or prune

  17. The Arctic Tree Line and Climate Change

    E-Print Network [OSTI]

    Blouin-Demers, Gabriel

    ­ Compared the spruce treeline between 1947 and 1989 ­ Used remote sensing applications ­ Mean temperature cover - Causes surface heating Forest Tundra Chapin et al., (2005), 10 Nadezhda et al., (2008) Albedo) ­Heating effect exceeds cooling effect ­Advances in the tree line are causing global warming to occur even

  18. Microstrip transmission line tapers on ferrites

    SciTech Connect (OSTI)

    Albuquerque, M.R.; dAssuncao, A.G.; Lima, F. [Departamento de Engenharia Eletrica, Universidade Federal do Rio Grande do Norte, 59072-970 Natal, RN (Brazil)] [Departamento de Engenharia Eletrica, Universidade Federal do Rio Grande do Norte, 59072-970 Natal, RN (Brazil)

    1997-04-01T23:59:59.000Z

    The spectral domain approach is employed to perform a theoretical investigation of tapered microstrip lines on magnetized ferrite substrates. A linear variation of the conducting strip width along the direction of propagation is considered. The analysis takes into account the effects of the applied dc magnetic bias field in the transmission characteristics of these structures. The properties of the taper are determined by a model based on a segmentation of the considered line into uniform microstrip line subsections. Normalized phase constants and characteristic impedances are obtained by using the Hertz vector potentials method and Galerkin numerical technique. Numerical results are presented to show the taper input parameters as a function of the load impedance, geometrical dimensions, operating frequency, and ferrite parameters, considering the orientation and magnitude changes of the biasing magnetic-field {bold H}{sub 0}. The results agree fairly well with those available in the literature for tapered microstrip lines on isotropic dielectric substrates. {copyright} {ital 1997 American Institute of Physics.}

  19. GLOBAL LINE PROFILE ASYMMETRIES IN DISK GALAXIES

    E-Print Network [OSTI]

    Bershady, Matthew A.

    GLOBAL LINE PROFILE ASYMMETRIES IN DISK GALAXIES David R. Andersen1, Matthew A. Bershady2 1NRC profiles of 39 disk galaxies with H i and H ii data. We find good agreement between the first and second mo- ments of the profiles and recession and rotation velocities, respectively. The shapes of H i and H ii

  20. Dielectronic recombination lines of C{sup +}

    SciTech Connect (OSTI)

    Sochi, Taha, E-mail: taha.sochi@kcl.ac.uk; Storey, Peter J.

    2013-11-15T23:59:59.000Z

    The present paper presents atomic data generated to investigate the recombination lines of C II in the spectra of planetary nebulae. These data include energies of bound and autoionizing states, oscillator strengths and radiative transition probabilities, autoionization probabilities, and recombination coefficients. The R-matrix method of electron scattering theory was used to describe the C{sup 2+} plus electron system.

  1. On line chemical composition of analyzer development

    SciTech Connect (OSTI)

    Not Available

    1992-01-01T23:59:59.000Z

    This status report details progress made on the development of an on-line chemical analyzer. Dynamic studies of the distillation column, approval of testing plans, installation of a sample cell and raman analyzer, design of computerized control systems are described. Information on seminars and published papers is also included. (GHH)

  2. Calibration for Frequencies of Stellar Spectral Lines

    E-Print Network [OSTI]

    Gomez, Juana

    2010-07-14T23:59:59.000Z

    to approximately three degrees Celsius. White Light-Emitting Diodes (LED?s) were used as light input. Light traveled through the bromine cell. The blue line corresponds to the initial measurement of the signal traveling through air, which only shows an almost...

  3. TRIBAND BRANCH LINE COUPLER USING DOUBLE-LORENTZ

    E-Print Network [OSTI]

    Nam, Sangwook

    TRIBAND BRANCH LINE COUPLER USING DOUBLE-LORENTZ TRANSMISSION LINES Hanseung Lee and Sangwook Nam) transmission lines (TL) provides two additional degrees of freedom in realizing triband microwave devices-Lorentz; transmission lines; triband; directional couplers 1. INTRODUCTION The concept of artificial TLs having

  4. Command Line Arguments in Ada95 Mark Allen Weiss

    E-Print Network [OSTI]

    Weiss, Mark Allen

    Command Line Arguments in Ada95 Mark Allen Weiss School of Computer Science Florida International (additional) arguments: ''­w'' and ''echo args.adb''. These are known as command line arguments. Command line on a command line can be accessed in an Ada program. In Ada83 there was no standard way of doing this. Ada95

  5. Line profile and continuum variability in the very broad-line Seyfert galaxy Mrk 926

    E-Print Network [OSTI]

    Kollatschny, W

    2010-01-01T23:59:59.000Z

    We present results of an intensive spectroscopic variability campaign of the very broad-line Seyfert 1 galaxy Mrk 926. Our aim is to investigate the broad-line region (BLR) by studying the intensity and line profile variations of this galaxy on short timescales. High signal-to-noise ratio(S/N) spectra were taken with the 9.2m Hobby-Eberly Telescope (HET) in identical conditions during two observing campaigns in 2004 and 2005. After the spectral reduction and internal calibration we achieved a relative flux accuracy of better than 1\\%. The rms profiles of the very broad Balmer lines have shapes that differ from their mean line profiles, consisting of two inner (v $\\lesssim \\pm{}$ 6~000 km s$^{-1}$) and two outer (v $\\gtrsim \\pm{}$ 6~000 km s$^{-1}$) line components in addition to a central component (v $\\lesssim \\pm{}$ 600 km s$^{-1}$). These outer and inner line segments varied with different amplitudes during our campaign. The radius of the BLR is very small with an upper limit of 2~light-days for the H$\\bet...

  6. Second-order susceptibility from a tight-binding Hamiltonian

    E-Print Network [OSTI]

    Dumitrica, T.; Graves, JS; Allen, Roland E.

    1998-01-01T23:59:59.000Z

    measurements. In Sec. PRB 580163-1829/98/58~23!/15340~4!/$15.00 a tight-binding Hamiltonian , and R. E. Allen , College Station, Texas 77843 June 1998! g Hamiltonian to include interaction with a time- l expression for the second-order susceptibility... potential A; however, the term that is neglected ~involving PRB 58 BRIEF REPORT A2) does not give rise to electronic transitions in the long- wavelength approximation, since it can be eliminated through a unitary transformation.15 Only the last term...

  7. Hydroxyapatite-binding peptides for bone growth and inhibition

    DOE Patents [OSTI]

    Bertozzi, Carolyn R. (Berkeley, CA); Song, Jie (Shrewsbury, MA); Lee, Seung-Wuk (Walnut Creek, CA)

    2011-09-20T23:59:59.000Z

    Hydroxyapatite (HA)-binding peptides are selected using combinatorial phage library display. Pseudo-repetitive consensus amino acid sequences possessing periodic hydroxyl side chains in every two or three amino acid sequences are obtained. These sequences resemble the (Gly-Pro-Hyp).sub.x repeat of human type I collagen, a major component of extracellular matrices of natural bone. A consistent presence of basic amino acid residues is also observed. The peptides are synthesized by the solid-phase synthetic method and then used for template-driven HA-mineralization. Microscopy reveal that the peptides template the growth of polycrystalline HA crystals .about.40 nm in size.

  8. Functionalized polymers for binding to solutes in aqueous solutions

    DOE Patents [OSTI]

    Smith, Barbara F.; Robison, Thomas W.

    2006-11-21T23:59:59.000Z

    A functionalized polymer for binding a dissolved molecule in an aqueous solution is presented. The polymer has a backbone polymer to which one or more functional groups are covalently linked. The backbone polymer can be such polymers as polyethylenimine, polyvinylamine, polyallylamine, and polypropylamine. These polymers are generally water-soluble, but can be insoluble when cross-linked. The functional group can be for example diol derivatives, polyol derivatives, thiol and dithiol derivatives, guest-host groups, affinity groups, beta-diphosphonic acids, and beta-diamides

  9. Characterization of Selective Binding of Alkali Cations with Carboxylate

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625govInstrumentstdmadapInactiveVisiting the TWPSuccessAlamosCharacterization of Selective Binding of Alkali Cations

  10. DNA-Binding Mechanism in Prokaryotic Partition Complex Formation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOEThe Bonneville Power Administration wouldDECOMPOSITION OF CALCIUM SULFATE: A REVIEWThis rcportJ itDNA-Binding

  11. Recombinant fusion protein of albumin-retinol binding protein inactivates stellate cells

    SciTech Connect (OSTI)

    Choi, Soyoung; Park, Sangeun; Kim, Suhyun [Laboratory of Cellular Oncology, Korea University Graduate School of Medicine, Ansan, Gyeonggi do 425-707 (Korea, Republic of)] [Laboratory of Cellular Oncology, Korea University Graduate School of Medicine, Ansan, Gyeonggi do 425-707 (Korea, Republic of); Lim, Chaeseung [Department of Laboratory Medicine, Korea University Guro Hospital, Seoul 152-703 (Korea, Republic of)] [Department of Laboratory Medicine, Korea University Guro Hospital, Seoul 152-703 (Korea, Republic of); Kim, Jungho [Department of Life Science, Sogang University, Seoul 121-742 (Korea, Republic of)] [Department of Life Science, Sogang University, Seoul 121-742 (Korea, Republic of); Cha, Dae Ryong [Department of Internal Medicine, Korea University Ansan Hospital, Ansan, Gyeonggi do 425-020 (Korea, Republic of)] [Department of Internal Medicine, Korea University Ansan Hospital, Ansan, Gyeonggi do 425-020 (Korea, Republic of); Oh, Junseo, E-mail: ohjs@korea.ac.kr [Laboratory of Cellular Oncology, Korea University Graduate School of Medicine, Ansan, Gyeonggi do 425-707 (Korea, Republic of)] [Laboratory of Cellular Oncology, Korea University Graduate School of Medicine, Ansan, Gyeonggi do 425-707 (Korea, Republic of)

    2012-02-03T23:59:59.000Z

    Highlights: Black-Right-Pointing-Pointer We designed novel recombinant albumin-RBP fusion proteins. Black-Right-Pointing-Pointer Expression of fusion proteins inactivates pancreatic stellate cells (PSCs). Black-Right-Pointing-Pointer Fusion proteins are successfully internalized into and inactivate PSCs. Black-Right-Pointing-Pointer RBP moiety mediates cell specific uptake of fusion protein. -- Abstract: Quiescent pancreatic- (PSCs) and hepatic- (HSCs) stellate cells store vitamin A (retinol) in lipid droplets via retinol binding protein (RBP) receptor and, when activated by profibrogenic stimuli, they transform into myofibroblast-like cells which play a key role in the fibrogenesis. Despite extensive investigations, there is, however, currently no appropriate therapy available for tissue fibrosis. We previously showed that the expression of albumin, composed of three homologous domains (I-III), inhibits stellate cell activation, which requires its high-affinity fatty acid-binding sites asymmetrically distributed in domain I and III. To attain stellate cell-specific uptake, albumin (domain I/III) was coupled to RBP; RBP-albumin{sup domain} {sup III} (R-III) and albumin{sup domain} {sup I}-RBP-albumin{sup III} (I-R-III). To assess the biological activity of fusion proteins, cultured PSCs were used. Like wild type albumin, expression of R-III or I-R-III in PSCs after passage 2 (activated PSCs) induced phenotypic reversal from activated to fat-storing cells. On the other hand, R-III and I-R-III, but not albumin, secreted from transfected 293 cells were successfully internalized into and inactivated PSCs. FPLC-purified R-III was found to be internalized into PSCs via caveolae-mediated endocytosis, and its efficient cellular uptake was also observed in HSCs and podocytes among several cell lines tested. Moreover, tissue distribution of intravenously injected R-III was closely similar to that of RBP. Therefore, our data suggest that albumin-RBP fusion protein comprises of stellate cell inactivation-inducing moiety and targeting moiety, which may lead to the development of effective anti-fibrotic drug.

  12. Pharmacological specificity of some psychotomimetic and antipsychotic agents for the sigma and PCP binding sites

    SciTech Connect (OSTI)

    Itzhak, Y.

    1988-01-01T23:59:59.000Z

    The pharmacological specificity of representative psychotomimetic agents such a phencyclidine (PCP) analogs, opiate benzomorphans and several antipsychotic agents was assessed for the sigma and PCP binding sites. In a series of binding experiments, in rat brain membranes, sigma and PCP binding sites were labeled with (/sup 3/H)-1-(1-(3-hydroxyphenyl) cyclohexyl) piperidine ((/sup 3/H)PCP-3-OH), (+)(/sup 3/H)-N-allylnormetazocine ((+)(/sup 3/H)SKF 10047) and (+) (/sup 3/H)-3-(3-hydroxy-phenyl)-N-(1-propyl) piperidine and ((+)(/sup 3/H)-3-PPP). PCP analogs inhibit potently high affinity (/sup 3/H)PCP-3-OH binding and (+)(/sup 3/H)SKF 10047 binding, moderately the low affinity binding component of (/sup 3/H)PCP-3-OH and very weakly (+) (/sup 3/H)-3-PPP binding. (+)SKF 10047 and cyclazocine are potent to moderate inhibitors of (+)(/sup 3/H)SKF 10047, high affinity (/sup 3/H)PCP-3-OH and (+)(/sup 3/H)-3-PCP-3-OH binding. The antipsychotic agents display high affinity for (+)(/sup 3/H)-3-PPP binding sites, moderate affinity for (+)(/sup 3/H)SKF 10047 sites and have no effect on either the high or low affinity (/sup 3/H)PCP-3-OH binding. 20 references, 3 figures, 2 tables.

  13. A tree macro Hideki ISOZAKI

    E-Print Network [OSTI]

    Mintmire, John W.

    ) to draw lines, use \\drawwith command before (or in) the bundle environment. \\drawwith{\\dashline[65{epic,ecltree} If your printer driver accepts tpic commands, you should specify eepic.sty after epic.sty. \\usepackage 3 Line attribute You can draw not only solid lines but also dotted lines and dash lines. epic

  14. Relativistic Nuclear Energy Density Functionals: adjusting parameters to binding energies

    E-Print Network [OSTI]

    T. Niksic; D. Vretenar; P. Ring

    2008-09-08T23:59:59.000Z

    We study a particular class of relativistic nuclear energy density functionals in which only nucleon degrees of freedom are explicitly used in the construction of effective interaction terms. Short-distance (high-momentum) correlations, as well as intermediate and long-range dynamics, are encoded in the medium (nucleon density) dependence of the strength functionals of an effective interaction Lagrangian. Guided by the density dependence of microscopic nucleon self-energies in nuclear matter, a phenomenological ansatz for the density-dependent coupling functionals is accurately determined in self-consistent mean-field calculations of binding energies of a large set of axially deformed nuclei. The relationship between the nuclear matter volume, surface and symmetry energies, and the corresponding predictions for nuclear masses is analyzed in detail. The resulting best-fit parametrization of the nuclear energy density functional is further tested in calculations of properties of spherical and deformed medium-heavy and heavy nuclei, including binding energies, charge radii, deformation parameters, neutron skin thickness, and excitation energies of giant multipole resonances.

  15. Binding and structure of tetramers in the scaling limit

    E-Print Network [OSTI]

    Hadizadeh, M R; Tomio, L; Delfino, A; Frederico, T

    2011-01-01T23:59:59.000Z

    The momentum-space structure of the Faddeev-Yakubovsky (FY) components of weakly-bound tetramers is investigated at the unitary limit using a renormalized zero-range two-body interaction. The results, obtained by considering a given trimer level with binding energy $B_3$, provide further support to a universal scaling function relating the binding energies of two successive tetramer states. The correlated scaling between the tetramer energies comes from the sensitivity of the four-boson system to a short-range four-body scale. Each excited $N-$th tetramer energy $B_4^{(N)}$ moves as the short-range four-body scale changes, while the trimer properties are kept fixed, with the next excited tetramer $B_4^{(N+1)}$ emerging from the atom-trimer threshold for a universal ratio $B_4^{(N)}/B_3 = B_4^ {(N)}/B_4^{(N+1)} \\simeq 4.6$, which does not depend on $N$. We show that both channels of the FY decomposition [atom-trimer ($K-$type) and dimer-dimer ($H-$type)] present high momentum tails, which reflect the short-ran...

  16. Binding and structure of tetramers in the scaling limit

    E-Print Network [OSTI]

    M. R. Hadizadeh; M. T. Yamashita; L. Tomio; A. Delfino; T. Frederico

    2012-01-17T23:59:59.000Z

    The momentum-space structure of the Faddeev-Yakubovsky (FY)components of weakly-bound tetramers is investigated at the unitary limit using a renormalized zero-range two-body interaction. The results, obtained by considering a given trimer level with binding energy $B_3$, provide further support to a universal scaling function relating the binding energies of two successive tetramer states. The correlated scaling between the tetramer energies comes from the sensitivity of the four-boson system to a short-range four-body scale. Each excited $N-$th tetramer energy $B_4^{(N)}$ moves as the short-range four-body scale changes, while the trimer properties are kept fixed, with the next excited tetramer $B_4^{(N+1)}$ emerging from the atom-trimer threshold for a universal ratio $B_4^{(N)}/B_3 = B_4^ {(N)}/B_4^{(N+1)} \\simeq 4.6$, which does not depend on $N$. We show that both channels of the FY decomposition [atom-trimer ($K-$type) and dimer-dimer ($H-$type)] present high momentum tails, which reflect the short-range four-body scale. We also found that the $H-$channel is favored over $K-$channel at low momentum when the four-body momentum scale largely overcomes the three-body one.

  17. Identification of protein binding sites in the promoter regions of a light-responsive gene family in a cyanobacterium

    E-Print Network [OSTI]

    Mueller, Ulrich Wolfgang

    1991-01-01T23:59:59.000Z

    binding sites were characterized that display some interdependence for protein binding ability. One binding site was identified as the primary sequence required for protein binding to the second site. Mutations were introduced into the first binding...lment of the requirements for the degree of MASTER OF SCIENCE December 1991 Major Subject: Biology ZDIBlTZPZCATION OP PROTEIN BINDINQ SITES IN TEB PROMOTER REGIONS OP A LIGHT RESPONSIVE SBBB FAMILY IN A CYANOBACTBRZUM A Thesis by ULRICH WOLFGANG...

  18. Multi-transmission-line-beam interactive system

    SciTech Connect (OSTI)

    Figotin, Alexander; Reyes, Guillermo [Department of Mathematics, University of California at Irvine, Irvine, California 92697-3875 (United States)] [Department of Mathematics, University of California at Irvine, Irvine, California 92697-3875 (United States)

    2013-11-15T23:59:59.000Z

    We construct here a Lagrangian field formulation for a system consisting of an electron beam interacting with a slow-wave structure modeled by a possibly non-uniform multiple transmission line (MTL). In the case of a single line we recover the linear model of a traveling wave tube due to J. R. Pierce. Since a properly chosen MTL can approximate a real waveguide structure with any desired accuracy, the proposed model can be used in particular for design optimization. Furthermore, the Lagrangian formulation provides: (i) a clear identification of the mathematical source of amplification, (ii) exact expressions for the conserved energy and its flux distributions obtained from the Noether theorem. In the case of uniform MTLs we carry out an exhaustive analysis of eigenmodes and find sharp conditions on the parameters of the system to provide for amplifying regimes.

  19. Giant vacuum forces via transmission lines

    E-Print Network [OSTI]

    Ephraim Shahmoon; Igor Mazets; Gershon Kurizki

    2014-07-23T23:59:59.000Z

    Quantum electromagnetic fluctuations induce forces between neutral particles, known as the van der Waals (vdW) and Casimir interactions. These fundamental forces, mediated by virtual photons from the vacuum, play an important role in basic physics and chemistry, and in emerging technologies involving, e.g. micro-electromechanical systems or quantum information processing. Here we show that these interactions can be enhanced by many orders of magnitude upon changing the character of the mediating vacuum-modes. By considering two polarizable particles in the vicinity of any standard electric transmission line, along which photons can propagate in one dimension (1d), we find a much stronger and longer-range interaction than in free-space. This enhancement may have profound implications on many-particle and bulk systems, and impact the quantum technologies mentioned above. The predicted giant vacuum force is estimated to be measurable in a coplanar waveguide line.

  20. Line-imaging Fabry-Perot interferometer

    SciTech Connect (OSTI)

    Mathews, A.R.; Warnes, R.H.; Hemsing, W.F.; Whittemore, G.R.

    1990-01-01T23:59:59.000Z

    A method for measuring the velocity history of a line element on a shock-loaded solid has been demonstrated. Light from single-frequency laser is focused through a cylindrical lens to a line on a moving target. The return Doppler-shifted image is passed through a Fabry-Perot interferometer. Because only specific combinations of incident light angle and frequency can pass through the interferometer the output is an incomplete image of the moving target appearing as a set of fringes. This image is focused onto an electronic streak camera and swept in time. The fringe pattern changes with time as the target surface moves, allowing determination of velocity for each point on the target that forms a fringe. Because the velocity can only be measured at the fringe positions, it is necessary to use an interpolating polynomial to obtain a continuous function of time and velocity along the sampled lien. 9 refs., 7 figs.

  1. In-line real time air monitor

    DOE Patents [OSTI]

    Wise, Marcus B. (Kingston, TN); Thompson, Cyril V. (Knoxville, TN)

    1998-01-01T23:59:59.000Z

    An in-line gas monitor capable of accurate gas composition analysis in a continuous real time manner even under strong applied vacuum conditions operates by mixing an air sample with helium forming a sample gas in two complementary sample loops embedded in a manifold which includes two pairs of 3-way solenoid valves. The sample gas is then analyzed in an ion trap mass spectrometer on a continuous basis. Two valve drivers actuate the two pairs of 3-way valves in a reciprocating fashion, so that there is always flow through the in-line gas monitor via one or the other of the sample loops. The duty cycle for the two pairs of 3-way valves is varied by tuning the two valve drivers to a duty cycle typically between 0.2 to 0.7 seconds.

  2. In-line real time air monitor

    DOE Patents [OSTI]

    Wise, M.B.; Thompson, C.V.

    1998-07-14T23:59:59.000Z

    An in-line gas monitor capable of accurate gas composition analysis in a continuous real time manner even under strong applied vacuum conditions operates by mixing an air sample with helium forming a sample gas in two complementary sample loops embedded in a manifold which includes two pairs of 3-way solenoid valves. The sample gas is then analyzed in an ion trap mass spectrometer on a continuous basis. Two valve drivers actuate the two pairs of 3-way valves in a reciprocating fashion, so that there is always flow through the in-line gas monitor via one or the other of the sample loops. The duty cycle for the two pairs of 3-way valves is varied by tuning the two valve drivers to a duty cycle typically between 0.2 to 0.7 seconds. 3 figs.

  3. Binding of transcription factors adapts to resolve information-energy trade-off

    E-Print Network [OSTI]

    Kagan, Yonatan Savir Jacob

    2015-01-01T23:59:59.000Z

    We examine the binding of transcription factors to DNA in terms of an information transfer problem. The input of the noisy channel is the biophysical signal of a factor bound to a DNA site, and the output is a distribution of probable DNA sequences at this site. This task involves an inherent tradeoff between the information gain and the energetics of the binding interaction - high binding energies provide higher information gain but hinder the dynamics of the system as factors are bound too tightly. We show that adaptation of the binding interaction towards increasing information transfer under energy constraints implies that the information gain per specific binding energy at each base-pair is maximized. We analyze hundreds of prokaryote and eukaryote transcription factors from various organisms to evaluate the discrimination energies. We find that, in accordance with our theoretical argument, binding energies nearly maximize the information gain per energy. This work suggests the adaptation of information ...

  4. Autoradiographic localization of endothelin-1 binding sites in the cardiovascular and respiratory systems

    SciTech Connect (OSTI)

    Power, R.F.; Wharton, J.; Zhao, Y.; Bloom, S.R.; Polak, J.M.

    1989-01-01T23:59:59.000Z

    Specific high-affinity binding sites for endothelin-1 (ET-1) have been demonstrated in peripheral tissues using the technique of in vitro receptor autoradiography. Binding was time dependent and saturable and inhibited by coincubation with an excess of unlabeled ET-1 but resistant to dissociation. Binding sites were localized to blood vessels of all sizes including coronary arteries, intrapulmonary vessels, and intrarenal and intrasplenic arteries. In addition, high-affinity binding sites were identified on airway smooth muscle, over alveolar septa, and on nerve trunks. Scatchard analysis of the data revealed a Bmax of 250 amol/mm2 and a Kd of 0.1 nM for the binding of rat tracheal smooth muscle, with similar values for porcine coronary artery. The localization of binding sites is consistent with the known effects of ET-1 and suggests a direct action on specific receptors.

  5. New Diagnostics in the FLASH Dump Line

    E-Print Network [OSTI]

    Line Status Aug. 2009 BPM 9DUMP BPM 15DUMP BPM 5DUMP Toroid 9DUMP OTR screen 9DUMP BLM 14DUMP BLM 13 at the dump #12;N. Baboi, MDIN. Baboi, MDI FLASH Seminar, Dec. 1, 2009FLASH Seminar, Dec. 1, 2009 Old BPM 15DUMPOld BPM 15DUMP ·Strange behavior of BPM signals measured impedance from end of cable (in bld. 49): L

  6. Straight-line separation of two polyhedra

    E-Print Network [OSTI]

    Wang, Su-Hua

    1992-01-01T23:59:59.000Z

    1992 ABSTRACT Straight-line Separation of Two Polyhedra. (December 1992) Su-Hua Wang, B. S. , National Chiao Tung University, Taiwan, R. O. C Chair of Advisory Committee: Dr. Jan Wolter The development of automated assembly planning systems has... INTRODUCTION I. A Context Assembly planning is the plan generation problem for assembly tasks and plays an important role in manufacturing systems. The goal of the assembly planning process is to find a plan for moving a set of parts which are initially...

  7. Speed-line for 3D animation

    E-Print Network [OSTI]

    Song, Won Chan

    2007-04-25T23:59:59.000Z

    point moved to a CV on the circle . . . . . . . . . . . . . . 38 39 Ending point placed between the starting point and the goal object . 38 40 Speed-lines with another cartoon effect . . . . . . . . . . . . . . . . . 41 ix FIGURE Page 41 Image sequences... from the result animation . . . . . . . . . . . . . . . 42 1 CHAPTER I INTRODUCTION One of the earliest artists who tried to convey dynamic movement in a static image is Hokusai Katsushika, who is one of the best known Ukiyo-e1 artists. He is called...

  8. Stand-off transmission lines and method for making same

    DOE Patents [OSTI]

    Tuckerman, D.B.

    1991-05-21T23:59:59.000Z

    Standoff transmission lines in an integrated circuit structure are formed by etching away or removing the portion of the dielectric layer separating the microstrip metal lines and the ground plane from the regions that are not under the lines. The microstrip lines can be fabricated by a subtractive process of etching a metal layer, an additive process of direct laser writing fine lines followed by plating up the lines or a subtractive/additive process in which a trench is etched over a nucleation layer and the wire is electrolytically deposited. Microstrip lines supported on freestanding posts of dielectric material surrounded by air gaps are produced. The average dielectric constant between the lines and ground plane is reduced, resulting in higher characteristic impedance, less crosstalk between lines, increased signal propagation velocities, and reduced wafer stress. 16 figures.

  9. Stand-off transmission lines and method for making same

    DOE Patents [OSTI]

    Tuckerman, David B. (Livermore, CA)

    1991-01-01T23:59:59.000Z

    Standoff transmission lines in an integrated circuit structure are formed by etching away or removing the portion of the dielectric layer separating the microstrip metal lines and the ground plane from the regions that are not under the lines. The microstrip lines can be fabricated by a subtractive process of etching a metal layer, an additive process of direct laser writing fine lines followed by plating up the lines or a subtractive/additive process in which a trench is etched over a nucleation layer and the wire is electrolytically deposited. Microstrip lines supported on freestanding posts of dielectric material surrounded by air gaps are produced. The average dielectric constant between the lines and ground plane is reduced, resulting in higher characteristic impedance, less crosstalk between lines, increased signal propagation velocities, and reduced wafer stress.

  10. Rubber linings -- Overview and new technology

    SciTech Connect (OSTI)

    Mehra, L. [ARDCO Corp., Denver, CO (United States); Polaski, E.L. [Lord Corp., Erie, PA (United States); Lewis, R.K. [Blair Rubber Co., Akron, OH (United States); Mauri, A. [A. Tamburini and Co., Via Tazzoli (Italy)

    1995-12-01T23:59:59.000Z

    The authors have covered at some length the basic steps involved in rubber lining. They have talked about the progress made in adhesives for lining. The new system in use now is far superior to previous systems. The new systems and the developments going on towards water-based adhesives are discussed. The authors briefly brought up the various types of rubber materials and new developments in terms of chlorobutyl-faced three-ply rubbers as well as development of EPDM-based compounds in Europe. The methods of vulcanization used have been discussed, including hot air vulcanizing which is prevalent in Europe. The development of self-vulcanizing rubber and the advantages in use of pre-cured rubber have been described. The development of new methods, techniques and products for rubber lining has been slow but sure. As can be expected, new product development costs are huge, requiring expert attention and participation. The possibilities are limitless and effort is forthcoming from various sources. There is a need for an interchange of ideas, and the involvement of NACE International and other professional bodies is acknowledged and appreciated.

  11. Vibration damping required for overhead lines

    SciTech Connect (OSTI)

    Richardson, A.S. Jr. [Research Consulting Associates, Lexington, MA (United States)] [Research Consulting Associates, Lexington, MA (United States)

    1995-04-01T23:59:59.000Z

    Transmission line engineers have sought an easy method for evaluating if external dampers are required on any new transmission line. In the past this question has been given to the damper manufacturer who has a vested interest in a affirmative answer. For single conductors (and for bundled conductors) the vibration level of a power line conductor is the result of a complicated aerodynamic process in which energy is taken into the motion from vortex action on the lee side of the conductor. The amount of energy taken in is a non-linear function of vibration amplitude, frequency, conductor diameter, and wind speed. The amount of energy taken out is a non-linear function of vibration amplitude, frequency, conductor mass, conductor tension, and a certain friction constant that varies from conductor to conductor. It is known that a small amount of vibration is allowed without endangering the conductor. This has been called the IEEE limit loop velocity, and is numerically equal to 200 mm/second. This paper explains a simple procedure, applicable to single conductors, which calculates a comparison of energy in vs. energy out. From this process it is shown how to select the initial tension in the conductor so that no external dampers are required.

  12. Transmission line environmental assessment guidance document

    SciTech Connect (OSTI)

    Jackson, J.; Pentecost, E.; Muzzarelli, J.

    1994-01-01T23:59:59.000Z

    Since 1939, U.S. utility companies have been required to obtain a Presidential Permit to construct electric transmission lines that cross a U.S. border and connect with a foreign utility. The purpose of this document is to provide Presidential Permit applicants with two types of guidance: (1) on the type of environmental and project descriptive information needed to assess the potential impacts of the proposed and alternative actions and (2) on compliance with applicable federal and state regulations. The main three chapters present information on the purpose and content of this document (Chapter 1); legislative, regulatory, and consultation requirements for transmission line interconnect projects (Chapter 2); and identification of basic transmission system design parameters and environmental data requirements for analysis of potential impacts of the proposed action (Chapter 3). Chapter 3 also includes information on possible techniques or measures to mitigate impacts. Appendix A presents an overview of NEPA requirements and DOE`s implementing procedures. Appendix B summarizes information on legislation that may be applicable to transmission line projects proposed in Presidential Permit applications.

  13. E-Print Network 3.0 - a-binding protein acbp6 Sample Search Results

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Biology and Medicine 3 Femtomole Mixer for Microsecond Kinetic Studies of Protein Folding Summary: kinetics using FRET with acyl-CoA binding protein. In protein folding,...

  14. Anthraquinone Photonuclease Structure Determines Its Mode of Binding to DNA and the Cleavage

    E-Print Network [OSTI]

    Williams, Loren

    Anthraquinone Photonuclease Structure Determines Its Mode of Binding to DNA and the Cleavage recently described a set of anthraquinone derivatives that act as photonucleases.6 Three classes

  15. U-227: bind-dyndb-ldap DN Escaping Flaw Lets Remote Users Deny Service

    Broader source: Energy.gov [DOE]

    A vulnerability has been reported in bind-dyndb-ldap, which can be exploited by malicious people to cause a DoS (Denial of Service).

  16. Prediction of the binding affinities of peptides to class II MHC using a ...

    E-Print Network [OSTI]

    andrew

    2010-01-18T23:59:59.000Z

    Characteristic properties of peptide binding to class II MHC molecules can be inferred from ... conserved hydrogen bonds with the MHC [2]. Peptide side chains

  17. active-site structure binding: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Threshold Occupancy and Specific Cation Binding Modes in the Hammerhead Ribozyme Active Site components to facilitate catalysis through electrostatic engineering. In the case of...

  18. Sequestering Uranium from Seawater: Binding Strength and Modes of Uranyl Complexes with Glutarimidedioxime

    E-Print Network [OSTI]

    Tian, Guoxin

    2013-01-01T23:59:59.000Z

    Sequestering uranium from seawater: binding strength andin sequestering uranium from seawater, forms strongExtraction of uranium from seawater is very challenging, not

  19. Reversible CO-binding to the Active Site of Nitrogenase | Stanford...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Reversible CO-binding to the Active Site of Nitrogenase Tuesday, March 31, 2015 All living organisms depend on the availability of nitrogen for incorporation into the basic...

  20. Comparison of crystal and solution hemoglobin binding of selected antigelling agents and allosteric modifiers

    SciTech Connect (OSTI)

    Mehanna, A.S.; Abraham, D.J. (Virginia Commonwealth Univ., Richmond (USA))

    1990-04-24T23:59:59.000Z

    This paper details comprehensive binding studies (solution and X-ray) of human hemoglobin A with a group of halogenated carboxylic acids that were investigated as potential antisickling agents. It is, to our knowledge, the first study to compare solution and crystal binding for a series of compounds under similar high-salt conditions used for cocrystallization. The compounds include ((3,4-dichlorobenzyl)oxy)acetic acid, ((p-bromobenzyl)oxy)acetic acid, clofibric acid, and bezafibrate. The location and stereochemistry of binding sites have been established by X-ray crystallography, while the number of binding sites and affinity constants were measured by using equilibrium dialysis. The observed crystal structures are consistent with the binding observed in solution and that the number of binding sites is independent of salt concentration, while the binding constant increases with increasing salt concentration. The studies also reveal that relatively small changes in the chemical structure of a drug molecule can result in entirely different binding sites on the protein. Moreover, the X-ray studies provide a possible explanation for the multiplicity in function exhibited by these compounds as allosteric modulators and/or antisickling agents. Finally, the studies indicate that these compounds bind differently to the R and T states of hemoglobin, and observation of special significance to the original design of these agents.

  1. Binding Energies of the Alpha Particle and the A=3 Isobars from a Theoretical Geometric Model

    E-Print Network [OSTI]

    Gustavo R. Gonzalez-Martin

    2008-05-03T23:59:59.000Z

    We assume a triple geometric structure for the electromagnetic nuclear interaction. This nuclear electromagnetism is used to calculate the binding energies of the alpha particle and the A=3 isobar nuclides. The approximation for the resultant wave equation which lead to the deuteron binding energy from the modified Mathieu equation for the radial eigenvalue equation also establishes proton-electron-proton magnetic bonds in these nuclides and determines their binding energies. Completely theoretical calculations give 28.5 Mev., 7.64 Mev. and 8.42 Mev. for the binding energies of the alpha particle, the helium 3 isotope and tritium respectively. These values admit correction factors due to the approximations made.

  2. atp-binding rna aptamer: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    the b-phosphate. Induced-fit therefore strengthens active; ATP-binding site; induced fit; free energy transduction; ground-state destabilization Yin, Y. Whitney 3 Mutant...

  3. Energy landscapes for protein folding, binding, and aggregation : simple funnels and beyond

    E-Print Network [OSTI]

    Cho, Samuel Sung-Il

    2007-01-01T23:59:59.000Z

    coordinates capture protein folding on smooth landscapes.in the Prediction of Protein Folding Kinetics. Proc. Natl.Landscapes for Protein Folding, Binding, and Aggregation:

  4. Radio-Frequency Interference (RFI) From Extra-High-Voltage (EHV) Transmission Lines

    E-Print Network [OSTI]

    Ellingson, Steven W.

    -current (HVAC) transmission lines; high-voltage direct-current (HVDC) transmission lines will be addressed

  5. On the nuclear interaction. Potential, binding energy and fusion reaction

    E-Print Network [OSTI]

    I. Casinos

    2008-05-22T23:59:59.000Z

    The nuclear interaction is responsible for keeping neutrons and protons joined in an atomic nucleus. Phenomenological nuclear potentials, fitted to experimental data, allow one to know about the nuclear behaviour with more or less success where quantum mechanics is hard to be used. A nuclear potential is suggested and an expression for the potential energy of two nuclear entities, either nuclei or nucleons, is developed. In order to estimate parameters in this expression, some nucleon additions to nuclei are considered and a model is suggested as a guide of the addition process. Coulomb barrier and energy for the addition of a proton to each one of several nuclei are estimated by taking into account both the nuclear and electrostatic components of energy. Studies on the binding energies of several nuclei and on the fusion reaction of two nuclei are carried out.

  6. Quasielastic electron-deuteron scattering in the weak binding approximation

    SciTech Connect (OSTI)

    Ethier, Jacob J. [William and Mary College, JLAB; Doshi, Nidhi P. [Carnegie Mellon University; Malace, Simona P. [JLAB; Melnitchouk, Wally [JLAB

    2014-06-01T23:59:59.000Z

    We perform a global analysis of all available electron-deuteron quasielastic scattering data using Q^2-dependent smearing functions that describe inclusive inelastic e-d scattering within the weak binding approximation. We study the dependence of the cross sections on the deuteron wave function and the off-shell extrapolation of the elastic electron-nucleon cross section, which show particular sensitivity at x >> 1. The excellent overall agreement with data over a large range of Q^2 and x suggest a limited need for effects beyond the impulse approximation, with the exception of the very high-x or very low-Q^2 regions, where short-distance effects in the deuteron become more relevant.

  7. Relativistic emission lines from accreting black holes - The effect of disk truncation on line profiles

    E-Print Network [OSTI]

    Müller, A; Mueller, Andreas; Camenzind, Max

    2004-01-01T23:59:59.000Z

    Relativistic emission lines generated by thin accretion disks around rotating black holes are an important diagnostic tool for testing gravity near the horizon. The iron K-line is of special importance for the interpretation of the X-ray emission of Seyfert galaxies, quasars and galactic X-ray binary systems. A generalized kinematic model is presented which includes radial drifts and non-Keplerian rotations for the line emitters. The resulting line profiles are obtained with an object-oriented ray tracer operating in the curved Kerr background metric. The general form of the Doppler factor is presented which includes all kinds of poloidal and toroidal motions near the horizon. The parameters of the model include the spin parameter, the inclination, the truncation and outer radius of the disk, velocity profiles for rotation and radial drift, the emissivity profile and a multi-species line-system. The red wing flux is generally reduced when radial drift is included as compared to the pure Keplerian velocity fie...

  8. Molecular modeling study on the effect of residues distant from the nucleotide-binding portion on RNA binding in Staphylococcus aureus Hfq

    E-Print Network [OSTI]

    Lee, Keun Woo

    of Quality of Life, Korea Research Institute of Standards and Science, Daejeon 305-340, Republic of Korea c region of the hexamer, and binds in a circular manner except for Journal of Molecular Graphics

  9. Radio-Frequency Inverters With Transmission-Line Input Networks

    E-Print Network [OSTI]

    Phinney, Joshua W.

    A soft-switching inverter topology (the Class Phi ) is presented which draws dc source current through a transmission line or a lumped-network approximation of a distributed line. By aligning the inverter switching frequency ...

  10. CHARACTERIZING LOSSES IN MICROSTRIP TRANSMISSION LINES Rashmi Pathak

    E-Print Network [OSTI]

    Timbie, Peter

    CHARACTERIZING LOSSES IN MICROSTRIP TRANSMISSION LINES by Rashmi Pathak A dissertation submitted . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 2.1 Transmission Lines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 2 Engineering) at the UNIVERSITY OF WISCONSIN­MADISON Summer 2005 #12;i Characterizing Losses in Transmission

  11. Mode conversation losses in overmolded millimeter wave transmission lines

    E-Print Network [OSTI]

    Tax, David S. (David Samuel)

    2008-01-01T23:59:59.000Z

    Millimeter wave transmission lines are integral components for many important applications like nuclear fusion and NMR spectroscopy. In low loss corrugated transmission lines propagating the HE,1 mode with a high waveguide ...

  12. EIS-0421: Big Eddy-Knight Transmission Line

    Broader source: Energy.gov [DOE]

    BPA is proposing to build a new 500 kilovolt (kV) transmission line in Wasco County, Oregon and Klickitat County, Washington and a new substation in Klickitat County. The new BPA transmission line...

  13. Lovell-Yellowtail transmission line rebuild project, Western...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    on Flickr. Western is rebuilding the Lovell-to-Yellowtail No. 1 and No. 2 115-kV transmission lines. The 94-circuit-miles of lines are located between Lovell, Wyo., and Yellowtail...

  14. PI Control of Gene Expression in Tumorous Cell Lines

    E-Print Network [OSTI]

    Mendonca, Rouella J.

    2010-01-16T23:59:59.000Z

    cancer cell line genes behave more like their Human Embryonic Kidney cell line counterparts. Two methods of intervention were introduced. The first method was the simpler on-off control intervention while the second method used a more advanced...

  15. Efficient buffer design algorithms for production line profit maximization

    E-Print Network [OSTI]

    Shi, Chuan, Ph. D. Massachusetts Institute of Technology

    2012-01-01T23:59:59.000Z

    A production line is a manufacturing system where machines are connected in series and separated by buffers. The inclusion of buffers increases the average production rate of the line by limiting the propagation of ...

  16. Secretary Chu Thanks Utility Staff and Line Workers For Their...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Thanks Utility Staff and Line Workers For Their Efforts to Restore Power in the Wake of Hurricane Sandy Secretary Chu Thanks Utility Staff and Line Workers For Their Efforts to...

  17. To: Mansueti, Lawrence Subject: RE: Pepco Scheduled Line Repair...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Sent: Monday, January 29, 2007 6:28 PM To: Mansueti, Lawrence Subject: RE: Pepco Scheduled Line Repair Dec. 1-20, 2006 Larry, Regarding the planned line outage,...

  18. Emailed Comments of Elizabeth Chimento RE: Pepco Scheduled Line...

    Office of Environmental Management (EM)

    Emailed Comments of Elizabeth Chimento RE: Pepco Scheduled Line Repair Dec. 1-20, 2006 Emailed Comments of Elizabeth Chimento RE: Pepco Scheduled Line Repair Dec. 1-20, 2006 Docket...

  19. Discriminating binding mechanisms of an intrinsically disordered protein via a multi-state coarse-grained model

    SciTech Connect (OSTI)

    Knott, Michael [Department of Chemistry, Cambridge University, Lensfield Road, Cambridge CB2 1EW (United Kingdom)] [Department of Chemistry, Cambridge University, Lensfield Road, Cambridge CB2 1EW (United Kingdom); Best, Robert B., E-mail: robertbe@helix.nih.gov [Department of Chemistry, Cambridge University, Lensfield Road, Cambridge CB2 1EW (United Kingdom); Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892-0520 (United States)

    2014-05-07T23:59:59.000Z

    Many proteins undergo a conformational transition upon binding to their cognate binding partner, with intrinsically disordered proteins (IDPs) providing an extreme example in which a folding transition occurs. However, it is often not clear whether this occurs via an “induced fit” or “conformational selection” mechanism, or via some intermediate scenario. In the first case, transient encounters with the binding partner favour transitions to the bound structure before the two proteins dissociate, while in the second the bound structure must be selected from a subset of unbound structures which are in the correct state for binding, because transient encounters of the incorrect conformation with the binding partner are most likely to result in dissociation. A particularly interesting situation involves those intrinsically disordered proteins which can bind to different binding partners in different conformations. We have devised a multi-state coarse-grained simulation model which is able to capture the binding of IDPs in alternate conformations, and by applying it to the binding of nuclear coactivator binding domain (NCBD) to either ACTR or IRF-3 we are able to determine the binding mechanism. By all measures, the binding of NCBD to either binding partner appears to occur via an induced fit mechanism. Nonetheless, we also show how a scenario closer to conformational selection could arise by choosing an alternative non-binding structure for NCBD.

  20. LINE Commission Visits Idaho National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOEThe Bonneville PowerCherries 82981-1cnHigh SchoolIn12electron 9 5 - -/e),,s - 1 2 3 4 5 6 7 84, LINE Commission

  1. On Line Journals - Links - Cyclotron Institute

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of Science (SC)IntegratedSpeeding access toTest and Evaluation | NationalNovemberOn-Line Journals

  2. CXD 4601, Line Yard Fence Project (4601)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office511041clothAdvanced Materials Advanced. C o w l i t z C o . C l a r8.0 -CURRICULUM VITAEAllen RadioLine

  3. SunLine Tests HHICE Bus in Desert Climate

    SciTech Connect (OSTI)

    Not Available

    2006-10-01T23:59:59.000Z

    Fact sheet describes the demonstration of a hybrid hydrogen internal combustion engine (HHICE) bus at SunLine Transit Agency.

  4. Defining the sequence specificity of Ikaros DNA binding zinc fingers and their role in T cell activation and tumor suppression

    E-Print Network [OSTI]

    Wadsworth, Sarah Elizabeth

    2013-01-01T23:59:59.000Z

    of  UNIX  platform  command-­?line   functions  that  lengths  (using  UNIX   command  line).    The  resulting  R/Bioconductor  command  lines  used  for   experimental  

  5. Reference: RGL 83-09 Subject: POWER TRANSMISSION LINES

    E-Print Network [OSTI]

    US Army Corps of Engineers

    Reference: RGL 83-09 Subject: POWER TRANSMISSION LINES Title: MARKING OF AERIAL POWER TRANSMISSION. 1. The marking of aerial power transmission lines, cables, and structures over the navigable waters LINES, CABLES, & STRUCTURES Issued: 07/13/83 Expires: 12/31/85 Originator: DAEN-CWO-N Description: ARMY

  6. Using Transmission Lines for Global On-Chip Communication

    E-Print Network [OSTI]

    Huang, Michael C.

    1 Using Transmission Lines for Global On-Chip Communication Aaron Carpenter, Jianyun Hu, Jie Xu-switched network-on-chip a less attractive option. An alternative solution uses well-engineered transmission lines as communication links. These transmission lines, along with simple, practical circuits using modern CMOS technol

  7. Asymmetric parametric amplification in nonlinear left-handed transmission lines

    E-Print Network [OSTI]

    Asymmetric parametric amplification in nonlinear left-handed transmission lines David A. Powell amplification in nonlinear left-handed transmission lines, which serve as model systems for nonlinear negative-handed regime. © 2009 American Institute of Physics. DOI: 10.1063/1.3089842 Left-handed transmission lines

  8. Multifrequency Wilkinson Power Divider Using Microstrip Nonuniform Transmission Lines

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    Multifrequency Wilkinson Power Divider Using Microstrip Nonuniform Transmission Lines Mohammad desired frequencies. The proposed structure contains two Microstrip Nonuniform Transmission Lines (MNTLs­6] and using composite right/left-handed transmission line (CRLH) [7]. The main disadvantage of multistage

  9. New approach for modelling distributed MEMS transmission lines

    E-Print Network [OSTI]

    Akin, Tayfun

    New approach for modelling distributed MEMS transmission lines K. Topalli, M. Unlu, S. Demir, O for the distributed MEMS transmission line (DMTL) structures. In this new model, the MEMS bridges that are used as the loading elements of the DMTL structures are represented as low-impedance transmission lines, rather than

  10. Evaluation of short-day onion doubled haploid lines

    E-Print Network [OSTI]

    Walker, Ryan Lee

    2009-05-15T23:59:59.000Z

    for pyruvic acid content ................................ 41 20 List of crosses made between two doubled haploid lines (H), two conventional lines (I), and one male sterile line (I11A)................................ 53 21 Means and LSD 0...), specific combining ability (SCA), reciprocal (REC), and nonmaternal (NMAT) effects for pyruvic acid content in onion .................. 71 37 Heterosis estimates of crosses grown at Uvalde, TX .................................... 72...

  11. Final Report 527 Economic Impact Analysis of Short Line Railroads

    E-Print Network [OSTI]

    Stephens, Jacqueline

    railroads. First described is the scope and presence of the eleven short line railroads currently operatingFinal Report 527 Economic Impact Analysis of Short Line Railroads by Jared J. Llorens, Ph.D. James's Catalog No. 4. Title and Subtitle Economic Impact Analysis of Short Line Railroads 5. Report Date October

  12. TOPCAT Plotting from STILTS API and Command Line

    E-Print Network [OSTI]

    Taylor, Mark

    TOPCAT Plotting from STILTS API and Command Line Mark Taylor, University of Bristol, UK your own Java application code, or from the STILTS command-line interface. Plot Model Each plot The STILTS package provides access to TOPCAT functions from the command line or a Jython front end. New

  13. Next Generation On-Line Dynamic Security Assessment

    E-Print Network [OSTI]

    Next Generation On-Line Dynamic Security Assessment Parts III and IV Final Project Report Power;Next Generation On-Line Dynamic Security Assessment Parts III and IV Final Project Report Parts III Research Center (PSERC) research project titled "Next Generation On-Line Dynamic Security Assessment

  14. Evidence for Line Broadening by Electron Scattering in the Broad Line Region of NGC 4395

    E-Print Network [OSTI]

    Ari Laor

    2006-01-30T23:59:59.000Z

    A high quality Keck spectrum of the Halpha line in NGC 4395 reveals symmetric exponential wings, fv\\propto e^{-v/sigma}, with sigma~500km/s. The wings extend out to >2500km/s from the line core, and down to a flux density of <10^-3 of the peak flux density. Numerical and analytic calculations indicate that exponential wings are expected for optically thin, isotropic, thermal electron scattering. Such scattering produces exponential wings with sigma=1.1sigma_e(ln tau_e^-1)^0.45, where sigma_e is the electron velocity dispersion, and tau_e is the electron scattering optical depth. The Halpha wings in NGC 4395 are well fit by an electron scattering model with tau_e=0.34, and an electron temperature T_e=1.1x10^4K. Such conditions are produced in photoionized gas with an ionization parameter U=0.3, as expected in the broad line region (BLR). Similar analysis of the [O III] 5007 line yields tau_e<0.01, consistent with the lower ionization in the narrow line region. If the electron scattering interpretation is correct, there should be a tight correlation between tau_e and the ionizing flux on time scales shorter than the BLR dynamical time, or ~1 week for NGC 4395. In contrast, the value of sigma should remain nearly constant on these time scales. Such wings may be discernible in other objects with unusually narrow Balmer lines, and they can provide a useful direct probe of T_e and tau_e in the BLR.

  15. USING DNASE DIGESTION DATA TO ACCURATELY IDENTIFY TRANSCRIPTION FACTOR BINDING SITES

    E-Print Network [OSTI]

    Hartemink, Alexander

    USING DNASE DIGESTION DATA TO ACCURATELY IDENTIFY TRANSCRIPTION FACTOR BINDING SITES KAIXUAN LUO1. But methods combining DNase digestion data with TF binding specificity information could potentially be used on the genomic digestion prod- ucts of deoxyribonuclease I (DNase I, which we will simply call DNase) might

  16. Chemical binding energies of point defects in palladium doped with hydrogen and d impurities

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    1001 Chemical binding energies of point defects in palladium doped with hydrogen and d impurities C calculate the chemical binding energy of a pair of hydrogen atoms in palladium within the infinite dilution] it is often assumed that the dominant contribution to the interaction energy between hydrogen atoms

  17. Measuring molecular rupture forces between single actin filaments and actin-binding proteins

    E-Print Network [OSTI]

    Kamm, Roger D.

    Measuring molecular rupture forces between single actin filaments and actin-binding proteins Jorge, and accepted by the Editorial Board April 24, 2008 (received for review June 29, 2007) Actin-binding proteins to model the mechanical properties of actin networks grown in vitro; however, there is a lack

  18. Direct calculation of the binding free energies of FKBP ligands Hideaki Fujitani,a

    E-Print Network [OSTI]

    Snow, Christopher

    Direct calculation of the binding free energies of FKBP ligands Hideaki Fujitani,a Yoshiaki Tanida energies of binding for eight ligands to FKBP protein were performed using the Fujitsu BioServer massively parallel computer. Using the latest version of the general assisted model building with energy refinement

  19. Kinetics of Bile Salt Binding to Liposomes Revealed by Carboxyfluorescein Release and

    E-Print Network [OSTI]

    Hinow, Peter

    Kinetics of Bile Salt Binding to Liposomes Revealed by Carboxyfluorescein Release and Mathematical by the binding of different bile salts to the leaflets of the lipid bilayer. We find that the permeability of the liposomal bilayer depends on the difference in the concentrations of bile salt in the inner and outer

  20. Binding and Translocation of Termination Factor Rho Studied at the Single-Molecule Level

    E-Print Network [OSTI]

    Straight, Aaron

    Binding and Translocation of Termination Factor Rho Studied at the Single-Molecule Level Daniel J; force spectroscopy Rho termination factor is an essential hexameric helicase responsible for terminating to investigate Rho­RNA binding in- teractions at the Rho utilization site of the tR1 terminator. Our results

  1. Gonadotropin binding sites in eel ovary : Autoradiographic visualization and new data on specificity

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    chorionic gonadotropin (hCG) binding was also observed consis- tently. Earlier data on the stimulation of in vitro cyclic AMP (cAMP) production by eel ovary led us to hypothesize that several types of GTH this hypothesis. The effects of an in vivo carp pituitary treatment on in vitro binding were also considered

  2. ON THE THERMODYNAMICS AND KINETICS OF THE COOPERATIVE BINDING OF BACTERIOPHAGE T4-

    E-Print Network [OSTI]

    Kowalczykowski, Stephen C.

    ON THE THERMODYNAMICS AND KINETICS OF THE COOPERATIVE BINDING OF BACTERIOPHAGE T4- CODED GENE 32 of thermodynamic, and preliminary kinetic, studies on the molecular details and specificity of interaction of phage into the molecular origins of binding cooperativity is obtained by determining these thermodynamic parameters also

  3. affinity brain membrane-binding: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    affinity brain membrane-binding First Page Previous Page 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 Next Page Last Page Topic Index 1 Membrane Binding of...

  4. A Novel Dimerization Interface of Cyclic Nucleotide Binding Domain, which is

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    1 A Novel Dimerization Interface of Cyclic Nucleotide Binding Domain, which is Disrupted Modeling 18, 9 (2012) 4053-4060" DOI : 10.1007/s00894-012-1404-5 #12;2 ABSTRACT Cyclic nucleotide binding and eukaryota. CNBD activation by cyclic nucleotide monophosphate (cNMP) is studied well in case of several

  5. Geometric Binding Site Design for Surface-Tension Driven Self-Assembly

    E-Print Network [OSTI]

    Geometric Binding Site Design for Surface-Tension Driven Self-Assembly Xiaorong Xiong, Sheng 98195-2500 Email: xrxiong@u.washington.edu Abstract-- Surface-tension driven self-assembly techniques-assembly, micro assembly, MEMS, hy- drophobic, hydrophilic, surface energy, surface tension force, binding site

  6. Control of stochasticity in magnetic field lines

    E-Print Network [OSTI]

    Cristel Chandre; Michel Vittot; Guido Ciraolo; Philippe Ghendrih; Ricardo Lima

    2005-11-03T23:59:59.000Z

    We present a method of control which is able to create barriers to magnetic field line diffusion by a small modification of the magnetic perturbation. This method of control is based on a localized control of chaos in Hamiltonian systems. The aim is to modify the perturbation locally by a small control term which creates invariant tori acting as barriers to diffusion for Hamiltonian systems with two degrees of freedom. The location of the invariant torus is enforced in the vicinity of the chosen target. Given the importance of confinement in magnetic fusion devices, the method is applied to two examples with a loss of magnetic confinement. In the case of locked tearing modes, an invariant torus can be restored that aims at showing the current quench and therefore the generation of runaway electrons. In the second case, the method is applied to the control of stochastic boundaries allowing one to define a transport barrier within the stochastic boundary and therefore to monitor the volume of closed field lines.

  7. Lorentz transformations with arbitrary line of motion

    E-Print Network [OSTI]

    Chandru Iyer; G. M. Prabhu

    2008-09-18T23:59:59.000Z

    Sometimes it becomes a matter of natural choice for an observer (A) that he prefers a coordinate system of two-dimensional spatial x-y coordinates from which he observes another observer (B) who is moving at a uniform speed along a line of motion, which is not collinear with As chosen x or y axis. It becomes necessary in such cases to develop Lorentz transformations where the line of motion is not aligned with either the x or the y-axis. In this paper we develop these transformations and show that under such transformations, two orthogonal systems (in their respective frames) appear non-orthogonal to each other. We also illustrate the usefulness of the transformation by applying it to three problems including the rod-slot problem. The derivation has been done before using vector algebra. Such derivations assume that the axes of K and K-prime are parallel. Our method uses matrix algebra and shows that the axes of K and K-prime do not remain parallel, and in fact K and K-prime which are properly orthogonal are observed to be non-orthogonal by K-prime and K respectively. http://www.iop.org/EJ/abstract/0143-0807/28/2/004

  8. Linee guida per l'utilizzo del logoLinee guida per l'utilizzo del logoLinee guida per l'utilizzo del logoLinee guida per l'utilizzo del logo d'Ateneod'Ateneod'Ateneod'Ateneo Universit degli Studi di Catania Linee guida per l'utilizzo del logo d'Ateneo

    E-Print Network [OSTI]

    Bella, Giampaolo

    Linee guida per l'utilizzo del logoLinee guida per l'utilizzo del logoLinee guida per l'utilizzo del logoLinee guida per l'utilizzo del logo d'Ateneod'Ateneod'Ateneod'Ateneo #12;Università degli Studi di Catania Linee guida per l'utilizzo del logo d'Ateneo Pag. 1 Premessa Negli ultimi anni

  9. Dynamics of intracellular Ca$^{2+}$ oscillations in the presence of multisite Ca$^{2+}$-binding proteins

    E-Print Network [OSTI]

    Roberto Chignola; Alessio Del Fabbro; Edoardo Milotti

    2009-09-10T23:59:59.000Z

    We study the dynamics of intracellular calcium oscillations in the presence of proteins that bind calcium on multiple sites and that are generally believed to act as passive calcium buffers in cells. We find that multisite calcium-binding proteins set a sharp threshold for calcium oscillations. Even with high concentrations of calcium-binding proteins, internal noise, which shows up spontaneously in cells in the process of calcium wave formation, can lead to self-oscillations. This produces oscillatory behaviors strikingly similar to those observed in real cells. In addition, for given intracellular concentrations of both calcium and calcium-binding proteins the regularity of these oscillations changes and reaches a maximum as a function noise variance, and the overall system dynamics displays stochastic coherence. We conclude that calcium-binding proteins may have an important and active role in cellular communication.

  10. A model study of cooperative binding of ionic surfactants to oppositely charged flexible polyions

    E-Print Network [OSTI]

    T. Nishio; T. Shimizu; Sh. Yoshida; A. Minakata

    2015-01-10T23:59:59.000Z

    A novel statistical model for the cooperative binding of monomeric ligands to a linear lattice is developed to study the interaction of ionic surfactant molecules with flexible polyion chain in dilute solution. Electrostatic binding of a ligand to a site on the polyion and hydrophobic associations between the neighboring bound ligands are assumed to be stochastic processes. Ligand association separated by several lattice points within defined width is introduced for the flexible polyion. Model calculations by the Monte Carlo method are carried out to investigate the binding behavior. The hypothesis on the ligand association and its width on the chain are of importance in determining critical aggregation concentration and binding isotherm. The results are reasonable for the interpretations of several surfactant-flexible polyion binding experiments. The implications of the approach are presented and discussed.

  11. The broadening of Lyman-alpha forest absorption lines

    E-Print Network [OSTI]

    Garzilli, Antonella; Schaye, Joop

    2015-01-01T23:59:59.000Z

    We provide an analytical description of the line broadening of HI absorbers in the Lyman-alpha forest resulting from Doppler broadening and Jeans smoothing. We demonstrate that our relation captures the dependence of the line-width on column density for narrow lines in z~3 mock spectra remarkably well. Broad lines at a given column density arise when the underlying density structure is more complex, and such clustering is not captured by our model. Our understanding of the line broadening opens the way to a new method to characterise the thermal state of the intergalactic medium and to determine the sizes of the absorbing structures.

  12. Recombination rates, Resonance Strengths and Line Profiles of Dielectronic Satellite Lines

    E-Print Network [OSTI]

    Nahar, Sultana Nurun

    , The Ohio State University, Columbus, OH 43210, USA nahar@astronomy.ohio-state.edu, pradhan@astronomy.ohio, labeled `a' through `v', via transitions of the type 1s2l2l 1s2 2l. These lines are seen at energies of the highly charged ions Ca XVIII, Fe XXIV, and Ni XXVI. In contrast to obtaining a single energy point

  13. Method for bonding a transmission line to a downhole tool

    DOE Patents [OSTI]

    Hall, David R. (Provo, UT); Fox, Joe (Spanish Fork, UT)

    2007-11-06T23:59:59.000Z

    An apparatus for bonding a transmission line to the central bore of a downhole tool includes a pre-formed interface for bonding a transmission line to the inside diameter of a downhole tool. The pre-formed interface includes a first surface that substantially conforms to the outside contour of a transmission line and a second surface that substantially conforms to the inside diameter of a downhole tool. In another aspect of the invention, a method for bonding a transmission line to the inside diameter of a downhole tool includes positioning a transmission line near the inside wall of a downhole tool and placing a mold near the transmission line and the inside wall. The method further includes injecting a bonding material into the mold and curing the bonding material such that the bonding material bonds the transmission line to the inside wall.

  14. The Infrared Ca II lines in Sunspot Umbrae

    E-Print Network [OSTI]

    Kollatschny, W; Wiehr, E; Fallipou, M A

    2012-01-01T23:59:59.000Z

    We present an empirical working model for sunspot umbrae which equally describes observed continuum intensities and line profiles. The wings of the infrared Ca II lines depend sensitively on the temperature gradient at -0.6 umbra and are thus insensitive to parasitic light. It is also shown that the infrared K I 7699 line is suitable for umbral spectroscopy since it is not seriously blended, its continuum is well defined and it is less influenced by parasitic light as compared to lines in the visible spectrum, due to the smaller umbal contrast. Calculations show that the umbral gradient dT/d(tau), required to fit the Ca II triplet lines, strongly conflicts with the observed profiles of K I 7699, NaD2 and Fe I 5434 (g=0), even when assuming vanishing Fe II lines for a maximum correction of parasitic light. It is shown that the discrepancy from the different line pr...

  15. Ground-based transmission line conductor motion sensor

    SciTech Connect (OSTI)

    Jacobs, M.L.; Milano, U.

    1988-08-22T23:59:59.000Z

    A ground-based-conductor motion-sensing apparatus is provided for remotely sensing movement of electric-power transmission lines, particularly as would occur during the wind-induced condition known as galloping. The apparatus is comprised of a motion sensor and signal-generating means which are placed underneath a transmission line and will sense changes in the electric field around the line due to excessive line motion. The detector then signals a remote station when a conditioning of galloping is sensed. The apparatus of the present invention is advantageous over the line-mounted sensors of the prior art in that it is easier and less hazardous to install. The system can also be modified so that a signal will only be given when particular conditions, such as specific temperature range, large-amplitude line motion, or excessive duration of the line motion, are occurring.

  16. XMM-Newton Observations of AGN Iron Line Profiles

    E-Print Network [OSTI]

    James Reeves

    2002-11-18T23:59:59.000Z

    XMM-Newton observations of type I AGN are presented. The properties of the iron K emission line are reviewed, the majority of AGN observed by XMM-Newton show narrow, unresolved (by XMM) iron lines at 6.4 keV from cold matter that must originate far from the inner accretion disc, perhaps in the putative torus or outer broad line region. The strength of this narrow line appears to decrease with luminosity, implying a reduction in the covering fraction of this material in the more luminous quasars. Few examples of the broad, relativistic iron line profile have been found by XMM-Newton, although in MCG -6-30-15, the extreme breadth of the broad line component may imply a Kerr metric for the central black hole. Generally, relativistic Fe K line profiles are not required in a number of other Seyfert 1 X-ray spectra.

  17. Nicotine induced CpG methylation of Pax6 binding motif in StAR promoter reduces the gene expression and cortisol production

    SciTech Connect (OSTI)

    Wang, Tingting [Department of Pharmacology, Basic Medical School of Wuhan University, Wuhan 430071 (China) [Department of Pharmacology, Basic Medical School of Wuhan University, Wuhan 430071 (China); Department of Pharmacology, Uniformed Services University of the Health Sciences, Bethesda, Maryland (United States); Chen, Man; Liu, Lian [Department of Pharmacology, Basic Medical School of Wuhan University, Wuhan 430071 (China)] [Department of Pharmacology, Basic Medical School of Wuhan University, Wuhan 430071 (China); Cheng, Huaiyan [Department of Pharmacology, Uniformed Services University of the Health Sciences, Bethesda, Maryland (United States)] [Department of Pharmacology, Uniformed Services University of the Health Sciences, Bethesda, Maryland (United States); Yan, You-E [Department of Pharmacology, Basic Medical School of Wuhan University, Wuhan 430071 (China)] [Department of Pharmacology, Basic Medical School of Wuhan University, Wuhan 430071 (China); Feng, Ying-Hong, E-mail: yhfeng@usuhs.edu [Department of Pharmacology, Uniformed Services University of the Health Sciences, Bethesda, Maryland (United States)] [Department of Pharmacology, Uniformed Services University of the Health Sciences, Bethesda, Maryland (United States); Wang, Hui, E-mail: wanghui19@whu.edu.cn [Department of Pharmacology, Basic Medical School of Wuhan University, Wuhan 430071 (China) [Department of Pharmacology, Basic Medical School of Wuhan University, Wuhan 430071 (China); Research Center of Food and Drug Evaluation, Wuhan University, Wuhan 430071 (China)

    2011-12-15T23:59:59.000Z

    Steroidogenic acute regulatory protein (StAR) mediates the rate-limiting step in the synthesis of steroid hormones, essential to fetal development. We have reported that the StAR expression in fetal adrenal is inhibited in a rat model of nicotine-induced intrauterine growth retardation (IUGR). Here using primary human fetal adrenal cortex (pHFAC) cells and a human fetal adrenal cell line NCI-H295A, we show that nicotine inhibits StAR expression and cortisol production in a dose- and time-dependent manner, and prolongs the inhibitory effect on cells proliferating over 5 passages after termination of nicotine treatment. Methylation detection within the StAR promoter region uncovers a single site CpG methylation at nt -377 that is sensitive to nicotine treatment. Nicotine-induced alterations in frequency of this point methylation correlates well with the levels of StAR expression, suggesting an important role of the single site in regulating StAR expression. Further studies using bioinformatics analysis and siRNA approach reveal that the single CpG site is part of the Pax6 binding motif (CGCCTGA) in the StAR promoter. The luciferase activity assays validate that Pax6 increases StAR gene expression by binding to the glucagon G3-like motif (CGCCTGA) and methylation of this site blocks Pax6 binding and thus suppresses StAR expression. These data identify a nicotine-sensitive CpG site at the Pax6 binding motif in the StAR promoter that may play a central role in regulating StAR expression. The results suggest an epigenetic mechanism that may explain how nicotine contributes to onset of adult diseases or disorders such as metabolic syndrome via fetal programming. -- Highlights: Black-Right-Pointing-Pointer Nicotine-induced StAR inhibition in two human adrenal cell models. Black-Right-Pointing-Pointer Nicotine-induced single CpG site methylation in StAR promoter. Black-Right-Pointing-Pointer Persistent StAR inhibition and single CpG methylation after nicotine termination. Black-Right-Pointing-Pointer Single CpG methylation located at Pax6 binding motif regulates StAR expression.

  18. A heuristic to generate rank-1 GMI cuts Sanjeeb Dash Mathematical ...

    E-Print Network [OSTI]

    2009-10-14T23:59:59.000Z

    Oct 14, 2009 ... branc h -and-bound to solv e an MI P arising in a practical ... f actors in h ibiting extensiv e generation o f GMI cuts in solv ing MI P s are t h at a f ...

  19. Cavity design and characteristics of monolithic long-wavelength InAs/InP quantum dash

    E-Print Network [OSTI]

    New Mexico, University of

    @unm.edu Abstract: By extending the net-gain modulation phasor approach to account for the discrete distribution. J. Malloy, and L. F. Lester, "Extremely Low Room-Temperature Threshold Current Density Diode Lasers­9 (1991). 13. Y.-C. Xin, Y. Li, A. Martinez, T. J. Rotter, H. Su, L. Zhang, A. L. Gray, S. Luong, K. Sun

  20. Performance of DASH and WebRTC Video Services for Mobile Users

    E-Print Network [OSTI]

    Panwar, Shivendra S.

    , and Opera. It specifies a standard Javascript API for enabling native voice and video communication standards that have been developed specifically to meet this demand and enable a high-quality experience industry bodies have been working to standardize the delivery of mobile video. In par- ticular, Dynamic

  1. Dash for Gas: The Sequel Christopher R. Knittel Konstantinos Metaxoglou Andre Trindade

    E-Print Network [OSTI]

    Rothman, Daniel

    in relative prices of the two fuels. Recent advances in the ability to use hydraulic fracturing methods to hydraulic fracturing for extracting shale gas. We focus on fuel switching decisions by electric power plants

  2. Demonstration of Datacenter Automation Software and Hardware (DASH) at the California Franchise Tax Board

    E-Print Network [OSTI]

    Bell, Geoffrey C.

    2010-01-01T23:59:59.000Z

    cooling units in a datacenter may be networked for remotecooling units in a datacenter may be networked for remote

  3. Demonstration of Datacenter Automation Software and Hardware (DASH) at the California Franchise Tax Board

    E-Print Network [OSTI]

    Bell, Geoffrey C.

    2010-01-01T23:59:59.000Z

    sensor. Figure 4: Open floor tiles. Figure 5: VFD installed.2. Repositioning floor tiles to improve air distribution. 3.are: re-arrangement of floor tiles; installation of variable

  4. Flow Test At Fenton Hill HDR Geothermal Area (Dash, 1989) | Open Energy

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directedAnnualPropertyd8c-a9ae-f8521cbb8489Information Hydro Inc Iosil

  5. Observation Wells At Fenton Hill HDR Geothermal Area (Dash, Et Al., 1983) |

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to: navigation, searchOfRoseConcerns Jumpsource HistoryFractures belowOasis PowerEnergyEnergyOpen

  6. Development Wells At Fenton Hill HDR Geothermal Area (Dash, Et Al., 1983) |

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directedAnnualPropertyd8c-a9ae-f8521cbb8489 No revision hasda62829c05bGabbs Type TermcitingOpen Energy

  7. Dispersion-free radial transmission lines

    DOE Patents [OSTI]

    Caporaso, George J. (Livermore, CA); Nelson, Scott D. (Patterson, CA)

    2011-04-12T23:59:59.000Z

    A dispersion-free radial transmission line ("DFRTL") preferably for linear accelerators, having two plane conductors each with a central hole, and an electromagnetically permeable material ("EPM") between the two conductors and surrounding a channel connecting the two holes. At least one of the material parameters of relative magnetic permeability, relative dielectric permittivity, and axial width of the EPM is varied as a function of radius, so that the characteristic impedance of the DFRTL is held substantially constant, and pulse transmission therethrough is substantially dispersion-free. Preferably, the EPM is divided into concentric radial sections, with the varied material parameters held constant in each respective section but stepwise varied between sections as a step function of the radius. The radial widths of the concentric sections are selected so that pulse traversal time across each section is the same, and the varied material parameters of the concentric sections are selected to minimize traversal error.

  8. Atomic line emission analyzer for hydrogen isotopes

    DOE Patents [OSTI]

    Kronberg, J.W.

    1991-05-08T23:59:59.000Z

    Apparatus for isotopic analysis of hydrogen comprises a low pressure chamber into which a sample of hydrogen is introduced and then exposed to an electrical discharge to excite the electrons of the hydrogen atoms to higher energy states and thereby cause the emission of light on the return to lower energy states, a Fresnel prism made at least in part of a material anomalously dispersive to the wavelengths of interest for dispersing the emitted light, and a photodiode array for receiving the dispersed light. The light emitted by the sample is filtered to pass only the desired wavelengths, such as one of the lines of the Balmer series for hydrogen, the wavelengths of which differ slightly from one isotope to another. The output of the photodiode array is processed to determine the relative amounts of each isotope present in the sample. Additionally, the sample itself may be recovered using, a metal hydride.

  9. Nonlinear transmission line based electron beam driver

    SciTech Connect (OSTI)

    French, David M.; Hoff, Brad W.; Tang Wilkin; Heidger, Susan; Shiffler, Don [Directed Energy Directorate, Air Force Research Laboratory, Kirtland AFB, New Mexico 87117 (United States); Allen-Flowers, Jordan [Program in Applied Mathematics, University of Arizona, Tucson, Arizona 85721 (United States)

    2012-12-15T23:59:59.000Z

    Gated field emission cathodes can provide short electron pulses without the requirement of laser systems or cathode heating required by photoemission or thermionic cathodes. The large electric field requirement for field emission to take place can be achieved by using a high aspect ratio cathode with a large field enhancement factor which reduces the voltage requirement for emission. In this paper, a cathode gate driver based on the output pulse train from a nonlinear transmission line is experimentally demonstrated. The application of the pulse train to a tufted carbon fiber field emission cathode generates short electron pulses. The pulses are approximately 2 ns in duration with emission currents of several mA, and the train contains up to 6 pulses at a frequency of 100 MHz. Particle-in-cell simulation is used to predict the characteristic of the current pulse train generated from a single carbon fiber field emission cathode using the same technique.

  10. Second Line of Defense Spares Program Assessment

    SciTech Connect (OSTI)

    Henderson, Dale L.; Muller, George; Mercier, Theresa M.; Brigantic, Robert T.; Perkins, Casey J.; Cooley, Scott K.

    2012-11-20T23:59:59.000Z

    The Office of the Second Line of Defense (SLD) is part of the Department of Energy‘s (DOE) National Nuclear Security Administration (NNSA). The SLD Program accomplishes its critical global security mission by forming cooperative relationships with partner countries to install passive radiation detection systems that augment traditional inspection and law enforcement measures by alerting border officials to the presence of special nuclear or other radiological materials in cross-border traffic. An important tenet of the program is to work collaboratively with these countries to establish the necessary processes, procedures, infrastructure and conditions that will enable them to fully assume the financial and technical responsibilities for operating the equipment. As the number of operational deployments grows, the SLD Program faces an increasingly complex logistics process to promote the timely and efficient supply of spare parts.

  11. Flavor instabilities in the neutrino line model

    E-Print Network [OSTI]

    Huaiyu Duan; Shashank Shalgar

    2015-05-28T23:59:59.000Z

    A dense neutrino medium can experience collective flavor oscillations through nonlinear neutrino-neutrino refraction. To make this multi-dimensional flavor transport problem more tractable, all existing studies have assumed certain symmetries (e.g., the spatial homogeneity and directional isotropy in the early universe) to reduce the dimensionality of the problem. In this work we show that, if both the directional and spatial symmetries are not enforced in the neutrino line model, collective oscillations can develop in the physical regimes where the symmetry-preserving oscillation modes are stable. Our results suggest that collective neutrino oscillations in real astrophysical environments (such as core-collapse supernovae and black-hole accretion discs) can be qualitatively different from the predictions based on existing models in which spatial and directional symmetries are artificially imposed.

  12. Calibrating transport lines using LOCO techniques

    SciTech Connect (OSTI)

    Yves Roblin

    2011-09-01T23:59:59.000Z

    With the 12GeV upgrade underway at CEBAF, there is a need to re-characterize the beamlines after the modifications made to it to accommodate running at higher energies. We present a linear perturbation approach to calibrating the optics model of transport lines. This method is adapted from the LOCO method in use for storage rings. We consider the effect of quadrupole errors, dipole construction errors as well as beam position monitors and correctors calibrations. The ideal model is expanded to first order in Taylor series of the quadrupole errors. A set of difference orbits obtained by exciting the correctors along the beamline is taken, yielding the measured response matrix. An iterative procedure is invoked and the quadrupole errors as well as beam position monitors and corrector calibration factors are obtained. Here we present details of the method and results of first measurements at CEBAF in early 2011.

  13. Atomic line emission analyzer for hydrogen isotopes

    DOE Patents [OSTI]

    Kronberg, James W. (108 Independent Blvd., Aiken, SC 29801)

    1993-01-01T23:59:59.000Z

    Apparatus for isotopic analysis of hydrogen comprises a low pressure chamber into which a sample of hydrogen is introduced and then exposed to an electrical discharge to excite the electrons of the hydrogen atoms to higher energy states and thereby cause the emission of light on the return to lower energy states, a Fresnel prism made at least in part of a material anomalously dispersive to the wavelengths of interest for dispersing the emitted light, and a photodiode array for receiving the dispersed light. The light emitted by the sample is filtered to pass only the desired wavelengths, such as one of the lines of the Balmer series for hydrogen, the wavelengths of which differ slightly from one isotope to another. The output of the photodiode array is processed to determine the relative amounts of each isotope present in the sample. Additionally, the sample itself may be recovered using a metal hydride.

  14. On-line tritium production monitor

    DOE Patents [OSTI]

    Mihalczo, J.T.

    1993-11-23T23:59:59.000Z

    A scintillation optical fiber system for the on-line monitoring of nuclear reactions in an event-by-event manner is described. In the measurement of tritium production one or more optical fibers are coated with enriched {sup 6}Li and connected to standard scintillation counter circuitry. A neutron generated {sup 6}Li(n)T reaction occurs in the coated surface of {sup 6}Li-coated fiber to produce energetic alpha and triton particles one of which enters the optical fiber and scintillates light through the fiber to the counting circuit. The coated optical fibers can be provided with position sensitivity by placing a mirror at the free end of the fibers or by using pulse counting circuits at both ends of the fibers. 5 figures.

  15. On-line tritium production monitor

    DOE Patents [OSTI]

    Mihalczo, John T. (Oak Ridge, TN)

    1993-01-01T23:59:59.000Z

    A scintillation optical fiber system for the on-line monitoring of nuclear reactions in an event-by-event manner is described. In the measurement of tritium production one or more optical fibers are coated with enriched .sup.6 Li and connected to standard scintillation counter circuitry. A neutron generated .sup.6 Li(n )T reaction occurs in the coated surface of .sup.6 Li-coated fiber to produce energetic alpha and triton particles one of which enters the optical fiber and scintillates light through the fiber to the counting circuit. The coated optical fibers can be provided with position sensitivity by placing a mirror at the free end of the fibers or by using pulse counting circuits at both ends of the fibers.

  16. Insulation spacer eliminates electric shorts between lines

    SciTech Connect (OSTI)

    Colaizzi, J.F.; Rockafellow, G.B.

    1983-03-01T23:59:59.000Z

    The design criteria incorporated into the pipeline insulating spacer were: spacer material selected must have a very large compressive and tensile strength in order to withstand the weight and stress resulting on the pipelines; provide the necessary abrasive resistance, dielectric strength, and will not decay underground; must not soften with heat when used around or near stream lines or will not cold flow under pressure; minimum length and circumference to reduce ''Shielding Effects'' from any cathodic protection system; and provide a material that incorporates a maximum strength at a minimum thickness. Explains that electric shorts are caused by 2 or more metallic structures in contact with each other. Notes that the insulating spacer's use has been expanded to provide electrical and physical insulation between carrier pipe and casing, supports for piping in compressing stations, and for pipelines that are suspended on bridges.

  17. Modelling receding contact lines on superhydrophobic surfaces

    E-Print Network [OSTI]

    B. M. Mognetti; J. M. Yeomans

    2010-10-23T23:59:59.000Z

    We use mesoscale simulations to study the depinning of a receding contact line on a superhydrophobic surface patterned by a regular array of posts. In order that the simulations are feasible, we introduce a novel geometry where a column of liquid dewets a capillary bounded by a superhydrophobic plane which faces a smooth hydrophilic wall of variable contact angle. We present results for the dependence of the depinning angle on the shape and spacing of the posts, and discuss the form of the meniscus at depinning. We find, in agreement with [17], that the local post concentration is a primary factor in controlling the depinning angle, and show that the numerical results agree well with recent experiments. We also present two examples of metastable pinned configurations where the posts are partially wet.

  18. Atomic line emission analyzer for hydrogen isotopes

    DOE Patents [OSTI]

    Kronberg, J.W.

    1993-03-30T23:59:59.000Z

    Apparatus for isotopic analysis of hydrogen comprises a low pressure chamber into which a sample of hydrogen is introduced and then exposed to an electrical discharge to excite the electrons of the hydrogen atoms to higher energy states and thereby cause the emission of light on the return to lower energy states, a Fresnel prism made at least in part of a material anomalously dispersive to the wavelengths of interest for dispersing the emitted light, and a photodiode array for receiving the dispersed light. The light emitted by the sample is filtered to pass only the desired wavelengths, such as one of the lines of the Balmer series for hydrogen, the wavelengths of which differ slightly from one isotope to another. The output of the photodiode array is processed to determine the relative amounts of each isotope present in the sample. Additionally, the sample itself may be recovered using a metal hydride.

  19. Binding Energy and the Fundamental Plane of Globular Clusters

    E-Print Network [OSTI]

    Dean E. McLaughlin

    2000-02-03T23:59:59.000Z

    A physical description of the fundamental plane of Galactic globular clusters is developed which explains all empirical trends and correlations in a large number of cluster observables and provides a small but complete set of truly independent constraints on theories of cluster formation and evolution in the Milky Way. Within the theoretical framework of single-mass, isotropic King models, it is shown that (1) 39 regular (non--core-collapsed) globulars with measured core velocity dispersions share a common V-band mass-to-light ratio of 1.45 +/- 0.10, and (2) a complete sample of 109 regular globulars reveals a very strong correlation between cluster binding energy and total luminosity, regulated by Galactocentric position: E_b \\propto (L^{2.05} r_{\\rm gc}^{-0.4}). The observational scatter about either of these two constraints can be attributed fully to random measurement errors, making them the defining equations of a fundamental plane for globular clusters. A third, weaker correlation, between total luminosity and the King-model concentration parameter, c, is then related to the (non-random) distribution of globulars on the plane. The equations of the FP are used to derive expressions for any cluster observable in terms of only L, r_{\\rm gc}, and c. Results are obtained for generic King models and applied specifically to the globular cluster system of the Milky Way.

  20. Structural basis of substrate discrimination and integrin binding by autotaxin

    SciTech Connect (OSTI)

    Hausmann, Jens; Kamtekar, Satwik; Christodoulou, Evangelos; Day, Jacqueline E.; Wu, Tao; Fulkerson, Zachary; Albers, Harald M.H.G.; van Meeteren, Laurens A.; Houben, Anna J.S.; van Zeijl, Leonie; Jansen, Silvia; Andries, Maria; Hall, Troii; Pegg, Lyle E.; Benson, Timothy E.; Kasiem, Mobien; Harlos, Karl; Vander Kooi, Craig W.; Smyth, Susan S.; Ovaa, Huib; Bollen, Mathieu; Morris, Andrew J.; Moolenaar, Wouter H.; Perrakis, Anastassis (Pfizer); (Leuven); (Oxford); (NCI-Netherlands); (Kentucky)

    2013-09-25T23:59:59.000Z

    Autotaxin (ATX, also known as ectonucleotide pyrophosphatase/phosphodiesterase-2, ENPP2) is a secreted lysophospholipase D that generates the lipid mediator lysophosphatidic acid (LPA), a mitogen and chemoattractant for many cell types. ATX-LPA signaling is involved in various pathologies including tumor progression and inflammation. However, the molecular basis of substrate recognition and catalysis by ATX and the mechanism by which it interacts with target cells are unclear. Here, we present the crystal structure of ATX, alone and in complex with a small-molecule inhibitor. We have identified a hydrophobic lipid-binding pocket and mapped key residues for catalysis and selection between nucleotide and phospholipid substrates. We have shown that ATX interacts with cell-surface integrins through its N-terminal somatomedin B-like domains, using an atypical mechanism. Our results define determinants of substrate discrimination by the ENPP family, suggest how ATX promotes localized LPA signaling and suggest new approaches for targeting ATX with small-molecule therapeutic agents.

  1. End-to-End Support for QoS-Aware Service Selection, Binding and Mediation in VRESCo

    E-Print Network [OSTI]

    Dustdar, Schahram

    to them (see Figure 1b). Service Contract Service Registry Service Provider Service Consumer Bind/Execute PublishFind (a) SOA Model Service Contract Service Provider Service Consumer Bind/Execute (b) SOA Practice

  2. Dynamic Nuclear Polarization Study of Inhibitor Binding to the M2[subscript 18–60] Proton Transporter from Influenza A

    E-Print Network [OSTI]

    Andreas, Loren B.

    We demonstrate the use of dynamic nuclear polarization (DNP) to elucidate ligand binding to a membrane protein using dipolar recoupling magic angle spinning (MAS) NMR. In particular, we detect drug binding in the proton ...

  3. THE JOURNAL OF CHEMICAL PHYSICS 134, 134701 (2011) Binding of hydrogen on benzene, coronene, and graphene from quantum

    E-Print Network [OSTI]

    Alfè, Dario

    2011-01-01T23:59:59.000Z

    THE JOURNAL OF CHEMICAL PHYSICS 134, 134701 (2011) Binding of hydrogen on benzene, coronene the binding energy curves of hydrogen on benzene, coronene, and graphene. The DMC results on benzene agree

  4. Sulfolobus shibatae CCA-adding Enzyme Forms a Tetramer upon Binding Two tRNA Molecules: A

    E-Print Network [OSTI]

    Li, Fang

    Sulfolobus shibatae CCA-adding Enzyme Forms a Tetramer upon Binding Two tRNA Molecules to form a tetramer is induced by the binding of two tRNA molecules. The formation of a tetramer with only

  5. On-line chemical composition analyzer development

    SciTech Connect (OSTI)

    Roberts, M.J.; Garrison, A.A.; Muly, E.C.; Moore, C.F.

    1992-02-01T23:59:59.000Z

    The energy consumed in distillation processes in the United States represents nearly three percent of the total national energy consumption. If effective control of distillation columns can be accomplished, it has been estimated that it would result in a reduction in the national energy consumption of 0.3%. Real-time control based on mixture composition could achieve these savings. However, the major distillation processes represent diverse applications and at present there does not exist a proven on-line chemical composition sensor technology which can be used to control these diverse processes in real-time. This report presents a summary of the findings of the second phase of a three phase effort undertaken to develop an on-line real-time measurement and control system utilizing Raman spectroscopy. A prototype instrument system has been constructed utilizing a Perkin Elmer 1700 Spectrometer, a diode pumped YAG laser, two three axis positioning systems, a process sample cell land a personal computer. This system has been successfully tested using industrially supplied process samples to establish its performance. Also, continued application development was undertaken during this Phase of the program using both the spontaneous Raman and Surface-enhanced Raman modes of operation. The study was performed for the US Department of Energy, Office of Industrial Technologies, whose mission is to conduct cost-shared R D for new high-risk, high-payoff industrial energy conservation technologies. Although this document contains references to individual manufacturers and their products, the opinions expressed on the products reported do not necessarily reflect the position of the Department of Energy.

  6. Acidic C-terminal tail of the ssDNA-binding protein of bacteriophage T7 and ssDNA

    E-Print Network [OSTI]

    Richardson, Charles C.

    Acidic C-terminal tail of the ssDNA-binding protein of bacteriophage T7 and ssDNA compete. Prokaryotic ssDNA-binding proteins share a conserved DNA-binding fold and an acidic C-terminal tail. It has been proposed that in the absence of ssDNA, the C-terminal tail contacts the ssDNA-binding cleft

  7. Homotypic clusters of transcription factor binding sites: a model system for understanding the physical mechanics of gene expression

    E-Print Network [OSTI]

    Ezer, Daphne; Zabet, Nicolae Radu; Adryan, Boris

    2014-08-01T23:59:59.000Z

    for studying complex CREs Recently, it has become possible to synthesize thousands of pro- moters or enhancers, and tomeasure the resulting level of gene expres- sion in parallel, an experimental design known as a massively parallel gene expression assay [9... heterotypic clusters influence gene expression. As the number of adjacent TF binding sites increases, the number of possible permutations of binding sites expands at a factorial scale. The distance between the binding sites and the order of the binding sites...

  8. Ropizine concurrently enhances and inhibits ( sup 3 H) dextromethorpan binding to different structures of the guinea pig brain: Autoradiographic evidence for multiple binding sites

    SciTech Connect (OSTI)

    Canoll, P.D.; Smith, P.R.; and Musacchio, J.M. (N.Y.U. Medical Center, New York (USA))

    1990-01-01T23:59:59.000Z

    Ropizine produces a simultaneous enhancement and inhibition of ({sup 3}H) dextromethorphan (DM) high-affinity binding to different areas of the guinea pig brain. These results imply that there are two distinct types of high-affinity ({sup 3}H)DM binding sites, which are present in variable proportions in different brain structures. The ropizine-enhances ({sup 3}H)DM binding type was preferentially inhibited by (+)-pentazocine. This is consistent with the presumption that the (+)-pentazocine-sensitive site is identical with the common site for DM and 3-(-3-Hydroxphenyl)-N-(1-propyl)piperidine ((+)-3-PPP). The second binding type, which is inhibited by ropizine and is not so sensitive to (+){minus} pentazocine, has not been fully characterized. This study demonstrates that the biphasic effects to ropizine are due, at least in part, to the effects of ropizine on two different types of ({sup 3}H)DM binding sites. However, this study does not rule out that the common DM/(+)-3-PPP site also might be inhibited by higher concentrations of ropizine.

  9. Receptor binding characteristics of tritiated misoprostol free acid in enriched canine parietal cells

    SciTech Connect (OSTI)

    Tsai, B.S.; Kessler, L.K.; Conway, R.G.; Schoenhard, G.; Stolzenbach, J.; Collins, P.; Kramer, S.; Butchko, G.M.; Bauer, R.F.

    1986-03-01T23:59:59.000Z

    Misoprostol (MISO) is a synthetic prostaglandin (PG) E/sub 1/ methyl ester with gastric antisecretory and mucosal protective properties. MISO is rapidly de-esterified to misoprostol free acid (MISO-FA) in enriched (65-80%) canine parietal cell preparations. Both forms appear to possess equivalent antisecretory potency and (/sup 3/H) MISO-FA is stable in these preparations. (/sup 3/H) MISO-FA binding was reversible and saturable with a maximal number of binding sites estimated at 8138 +/- 1893 per cell. The scatchard plot was linear, indicating a single, high affinity receptor population with a dissociation constant of 11 +/- 2.6 x 10/sup -9/ M. Unlabeled MISO-FA and MISO were equally potent inhibitors (IC/sub 50/, approx. 10/sup -8/M) of (/sup 3/H) MISO-FA binding. At 10/sup -5/ M, the dinor and tetranor ..beta..-oxidation metabolites of MISO were weak binding inhibitors. Strict stereospecific binding was shown by MISO stereoisomers, and the 11R, 16S isomer was most active. Both PGE/sub 1/ and 16,16 dimethyl PGE/sub 2/ were potent binding inhibitors, but PGF/sub 1/..cap alpha.. (10/sup -6/ M) and Hoe 892 (10/sup -5/ M), a stable PGI/sub 2/ analog, were weak inhibitors. Neither histamine or cimetidine competed for binding sites. These data indicate the presence of stereospecific E-type prostaglandin receptors in enriched canine parietal cell preparations.

  10. NMR studies of DNA oligomers and their interactions with minor groove binding ligands

    SciTech Connect (OSTI)

    Fagan, P A [Univ. of California, Berkeley, CA (United States). Dept. of Chemistry

    1996-05-01T23:59:59.000Z

    The cationic peptide ligands distamycin and netropsin bind noncovalently to the minor groove of DNA. The binding site, orientation, stoichiometry, and qualitative affinity of distamycin binding to several short DNA oligomers were investigated by NMR spectroscopy. The oligomers studied contain A,T-rich or I,C-rich binding sites, where I = 2-desaminodeoxyguanosine. I{center_dot}C base pairs are functional analogs of A{center_dot}T base pairs in the minor groove. The different behaviors exhibited by distamycin and netropsin binding to various DNA sequences suggested that these ligands are sensitive probes of DNA structure. For sites of five or more base pairs, distamycin can form 1:1 or 2:1 ligand:DNA complexes. Cooperativity in distamycin binding is low in sites such as AAAAA which has narrow minor grooves, and is higher in sites with wider minor grooves such as ATATAT. The distamycin binding and base pair opening lifetimes of I,C-containing DNA oligomers suggest that the I,C minor groove is structurally different from the A,T minor groove. Molecules which direct chemistry to a specific DNA sequence could be used as antiviral compounds, diagnostic probes, or molecular biology tools. The author studied two ligands in which reactive groups were tethered to a distamycin to increase the sequence specificity of the reactive agent.

  11. Putative Zinc Finger Protein Binding Sites Are Over-Represented in the Boundaries of Methylation-Resistant

    E-Print Network [OSTI]

    Putative Zinc Finger Protein Binding Sites Are Over- Represented in the Boundaries of Methylation that there are several over-represented putative Transcription Factor Binding Sites (TFBSs) in methylation-resistant CpG islands, and a specific group of zinc finger protein binding sites are over-represented in boundary

  12. Biochemistry 1994, 33, 13977-13988 13977 Effect of Conformational Flexibility and Solvation on Receptor-Ligand Binding

    E-Print Network [OSTI]

    Vajda, Sandor

    on Receptor-Ligand Binding Free Energies+ Sandor Vajda, Zhiping Weng, Rakefet Rosenfeld, and Charles De is presented for determing the free energy change accompanying ligand binding to protein receptors. The most conformational change on binding, to the free energy. Flexibility introduces two additional terms in the free

  13. Biochemistry 1994,33, 14565-14578 14565 Kinetic Mechanism of Adenine Nucleotide Binding to and Hydrolysis by the

    E-Print Network [OSTI]

    Lohman, Timothy M.

    Biochemistry 1994,33, 14565-14578 14565 Kinetic Mechanism of Adenine Nucleotide Binding nucleotides, we have investigated the kinetic mechanism of adenine nucleotide binding to the Rep monomer not significantly change the intrinsic tryptophan fluorescence, the binding of the fluorescent nucleotide analogue

  14. 1 Plasmodium falciparum SSB Tetramer Binds 2 Single-Stranded DNA Only in a Fully Wrapped Mode

    E-Print Network [OSTI]

    Lohman, Timothy M.

    1 Plasmodium falciparum SSB Tetramer Binds 2 Single-Stranded DNA Only in a Fully Wrapped Mode 3 with numerous DNA repair and replication proteins. Ec- 24 SSB tetramers can bind ssDNA in multiple DNA binding in fully wrapped complexes with site sizes of 30 52­65 nt/tetramer. Pf-SSB does not transition to the more

  15. GABA{sub A} receptor open-state conformation determines non-competitive antagonist binding

    SciTech Connect (OSTI)

    Chen Ligong [Environmental Chemistry and Toxicology Laboratory, Department of Environmental Science, Policy and Management, University of California, Berkeley, CA 94720 (United States); Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, CA 94158 (United States); Xue Ling [Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720 (United States); Giacomini, Kathleen M. [Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, CA 94158 (United States); Casida, John E., E-mail: ectl@berkeley.edu [Environmental Chemistry and Toxicology Laboratory, Department of Environmental Science, Policy and Management, University of California, Berkeley, CA 94720 (United States)

    2011-02-01T23:59:59.000Z

    The {gamma}-aminobutyric acid (GABA) type A receptor (GABA{sub A}R) is one of the most important targets for insecticide action. The human recombinant {beta}3 homomer is the best available model for this binding site and 4-n-[{sup 3}H]propyl-4'-ethynylbicycloorthobenzoate ([{sup 3}H]EBOB) is the preferred non-competitive antagonist (NCA) radioligand. The uniquely high sensitivity of the {beta}3 homomer relative to the much-less-active but structurally very-similar {beta}1 homomer provides an ideal comparison to elucidate structural and functional features important for NCA binding. The {beta}1 and {beta}3 subunits were compared using chimeragenesis and mutagenesis and various combinations with the {alpha}1 subunit and modulators. Chimera {beta}3/{beta}1 with the {beta}3 subunit extracellular domain and the {beta}1 subunit transmembrane helices retained the high [{sup 3}H]EBOB binding level of the {beta}3 homomer while chimera {beta}1/{beta}3 with the {beta}1 subunit extracellular domain and the {beta}3 subunit transmembrane helices had low binding activity similar to the {beta}1 homomer. GABA at 3 {mu}M stimulated heteromers {alpha}1{beta}1 and {alpha}1{beta}3 binding levels more than 2-fold by increasing the open probability of the channel. Addition of the {alpha}1 subunit rescued the inactive {beta}1/{beta}3 chimera close to wildtype {alpha}1{beta}1 activity. EBOB binding was significantly altered by mutations {beta}1S15'N and {beta}3N15'S compared with wildtype {beta}1 and {beta}3, respectively. However, the binding activity of {alpha}1{beta}1S15'N was insensitive to GABA and {alpha}1{beta}3N15'S was stimulated much less than wildtype {alpha}1{beta}3 by GABA. The inhibitory effect of etomidate on NCA binding was reduced more than 5-fold by the mutation {beta}3N15'S. Therefore, the NCA binding site is tightly regulated by the open-state conformation that largely determines GABA{sub A} receptor sensitivity. - Graphical Abstract: Display Omitted Research Highlights: > The {beta}1 and {beta}3 subunits were compared by chimeragenesis, mutagenesis and modulators. > Low {beta}1 NCA binding was rescued by replacing its transmembrane helices with those of {beta}3. > GABA at 3 {mu}M stimulated heteromers {alpha}1{beta}1 and {alpha}1{beta}3 binding levels more than 2-fold. > Mutation at 15' position in TM2 reduced GABA stimulation of NCA binding. > The open-state conformation largely determines GABAA receptor sensitivity to NCAs.

  16. Method for detecting binding events using micro-X-ray fluorescence spectrometry

    DOE Patents [OSTI]

    Warner, Benjamin P. (Los Alamos, NM); Havrilla, George J. (Los Alamos, NM); Mann, Grace (Hong Kong, HK)

    2010-12-28T23:59:59.000Z

    Method for detecting binding events using micro-X-ray fluorescence spectrometry. Receptors are exposed to at least one potential binder and arrayed on a substrate support. Each member of the array is exposed to X-ray radiation. The magnitude of a detectable X-ray fluorescence signal for at least one element can be used to determine whether a binding event between a binder and a receptor has occurred, and can provide information related to the extent of binding between the binder and receptor.

  17. New binding materials for metal hydride electrodes which permit good recyclability

    SciTech Connect (OSTI)

    Hara, T.; Yasuda, N. (Japan Synthetic Rubber Co., Ltd., Yokkaichi (Japan). Development Center); Takeuchi, Y. (Japan Synthetic Rubber Co., Ltd., Tokyo (Japan). Electronics Project Dept.); Sakai, T.; Uchiyama, A.; Miyamura, H.; Kuriyama, N.; Ishikawa, H. (Government Industrial Research Inst., Osaka (Japan))

    1993-09-01T23:59:59.000Z

    Thermoplastic elastomers such as styrene-butadiene-styrene block copolymer (SBS) and styrene-ethylene/butylene-styrene block copolymer (SEBS) were used successfully as binding materials for metal hydride (MH) electrodes of a nickel-metal hydride battery. These binding materials have a rubber-like nature and are soluble in organic solvents. It was easy to remove the alloy powder from a used electrode for recycling. The battery performance depended on both the kind and amount of binding materials. The best discharge capacity and rate capability were obtained for MH electrodes containing 2--5 weight percent (w/o) SEBS. The particle size distributions for the alloy were examined successfully.

  18. FOXM1 binds directly to non-consensus sequences in the human genome

    E-Print Network [OSTI]

    Sanders, Deborah A.; Gormally, Michael V.; Marsico, Giovanni; Beraldi, Dario; Tannahill, David; Balasubramanian, Shankar

    2015-06-23T23:59:59.000Z

    , Addenbrooke’s Hospital, Hills Road, Cambridge CB2 0SP, UK 4 Present address: Domainex, 162 Cambridge Science Park, Milton Road, Cambridge CB4 0GH, UK Abstract Background The Forkhead (FKH) transcription factor FOXM1 is a key regulator of the cell cycle... . Additionally, some transcription factors show different modes of recruitment to chromatin at specific sub-sets of genomic binding sites. For example, the ETS family member ELK1 [23] has two distinct types of binding modes, either binding redundantly...

  19. Partial proteolytic digestion of the mammary prolactin receptor: Identification of smaller prolactin binding fragments

    SciTech Connect (OSTI)

    Dusanter-Fourt, I.; Kelly, P.A.; Djiane, J. (Institut National de la Recherche Agronomique, Jouy-en-Josas (France))

    1990-01-01T23:59:59.000Z

    Partial proteolytic digestion of the mammary prolactin (PRL) receptor was used to generate receptor fragments and analyze their immunoreactivity and PRL binding properties. Tryptic digestion of the PRL receptor produced two immunoreactive fragments (Mr approximately 30,000 and approximately 15,000) that reacted with a monoclonal anti-PRL receptor antibody and still specifically bound PRL, while the complete immunoreactive PRL binding unit (Mr approximately 42,000) disappeared. Neither chymotrypsin nor V8 protease were able to generate any immunoreactive receptor fragments. These receptor fragments may represent smaller PRL binding receptor form(s) of biological significance.

  20. Energy measurement utilizing on-line chromatograph

    SciTech Connect (OSTI)

    Kizer, P.E. [Applied Automation, Inc./Hartmann and Braun, Houston, TX (United States)

    1995-12-01T23:59:59.000Z

    Most gas contracts today have at least a BTU specification and many use MMBTU (million BTU) rather than gas volume for custody transfer measurement. Gas chromatography is today being chosen more and more because the calculations of the gas volumes in modem electronic flow meters requires not only BTU{sub 5} information, but specific gravity, Mol % CO{sub 2} and Mol % N{sub 2}. The new AGA-8 supercompressibility equations also require a complete hydrocarbon analysis. What then, is a BTU? BTU is the acronym for British Thermal Unit. One BTU is the quantity of heat required to raise the temperature of one pound of water from 58.5{degrees}F to 59.5{degrees}F (about 1055.056 joules (SI))3. The higher the BTU content, the more energy can be obtained from burning the gas. It just doesn`t take as many cubic feet of gas to heat the home hot water tank if the gas is 1090 BTU instead of 940 BTU per SCF. The BTU, then, is a prime indicator of natural gas quality. An MMBTU{sup 2} is calculated by: BTU/CF * MMCF = MMBTU What is it worth to keep track of the natural gas BTU? If we postulate 1000 BTU/CF as fairly average for natural gas, and {+-} 5% error between doing a lab determination of the heating value on a spot sample of the gas and an on- line (nearly continuous) monitor of the heating value, this results in a {+-} 50 BTU difference. On a station that has 50 MMCF per day at $2.50 per MCF or MMBTU, this is $125,000.00 worth of gas per day. Five percent of this is $6,250.00 per day. If a process chromatograph, $50,000 installed cost, is used to determine the energy content a pay out of less than 10 days is obtained on a 50 MMCF/day station. Most major interconnects have on- line BTU measurement of some sort today.

  1. Sensor, method and system of monitoring transmission lines

    DOE Patents [OSTI]

    Syracuse, Steven J.; Clark, Roy; Halverson, Peter G.; Tesche, Frederick M.; Barlow, Charles V.

    2012-10-02T23:59:59.000Z

    An apparatus, method, and system for measuring the magnetic field produced by phase conductors in multi-phase power lines. The magnetic field measurements are used to determine the current load on the conductors. The magnetic fields are sensed by coils placed sufficiently proximate the lines to measure the voltage induced in the coils by the field without touching the lines. The x and y components of the magnetic fields are used to calculate the conductor sag, and then the sag data, along with the field strength data, can be used to calculate the current load on the line and the phase of the current. The sag calculations of this invention are independent of line voltage and line current measurements. The system applies a computerized fitter routine to measured and sampled voltages on the coils to accurately determine the values of parameters associated with the overhead phase conductors.

  2. Hyperreal Waves on Transfinte, Terminated, Distortionless and Lossless, Transmission Lines

    E-Print Network [OSTI]

    A. H. Zemanian

    2004-06-10T23:59:59.000Z

    A prior work (see Chapter 8 of the book, ``Graphs and Networks: Transfinite and Nonstandard,'' Birkhauser-Boston, Cambridge, Mass., USA, 2004) examined the propagation of an electromagnetic wave on a transfinite transmission line, transfinite in the sense that infinitely many one-way infinite transmission lines are connected in cascade. That there are infinitely many such one-way infinite lines results in the wave propagating without ever reflecting at some discontinuity. The present work examines the cascade where the cascade terminates after only finitely many one-way infinite transmission lines, with the result that reflected waves are now produced at both the far end as well as at the initial end of the transfinite transmission line. The question of whether the reflected waves are infini tesimal or appreciable and whether they sum to an infinitesimal or appreciable amount are resolved for both distortionless and lossless lines. Finally, the generalizations to higher ranks of transfiniteness is briefly summarized.

  3. Radio frequency communication system utilizing radiating transmission lines

    DOE Patents [OSTI]

    Struven, Warren C. (San Carlos, CA)

    1984-01-01T23:59:59.000Z

    A radio communication system for use in tunnels, mines, buildings or other shielded locations in which a pair of radiating transmission lines (30), (31) extend through such location in spaced coextensive relation to each other. Each transmission line (30), (31) has at least one unidirectional amplifier (32), (33) interposed therein with the sense of the unidirectional amplifier (32) of one transmission line (30) being opposite to the sense of the unidirectional amplifier (33) of the other transmission line (31). Each of the amplifiers (32), (33) has a gain which is less than the coupling loss between the transmission lines (30), (31). Two or more mobile transceivers (35) in the location served by the system are coupled to the transmission lines (30), (31) by electromagnetic wave propagation in space in order to communicate directly with each other at a given radio frequency within the frequency range of the system.

  4. Introduction to LINE: a Feynman diagram graphic generator

    SciTech Connect (OSTI)

    LeClaire, B.

    1985-03-01T23:59:59.000Z

    This is an exec for making Feynman graphs of amazing complexity with amazing simplicity - once you know how. And knowing how is hopefully quite easy to learn. The LINE exec works iteratively, repeatedly prompting you for the elements of the graph. You tell the exec what kinds of lines (or loops) to draw, and where to put them. Each line is plotted in a 10 x 10 Cartesian coordinate system using two ordered pairs of coordinates, (x/sub 1/,y/sub 1/) and (x/sub 2/,y/sub 2/), as endpoints. The types of lines that can be drawn include fermions, scalars, photons, gluons, W or Z bosons. As each line is added the exec alows you to display the updated picture, and to erase the last line added (if necessary). In this way you can keep going, drawing and erasing, until you have exactly what you want.

  5. DOE-EPRI On-Line Monitoring Implementation Guidelines

    SciTech Connect (OSTI)

    E. Davis; R. Bickford

    2003-01-02T23:59:59.000Z

    OAK B139 Industry and EPRI experience at several plants has shown on-line monitoring to be very effective in identifying out-of-calibration instrument channels or indications of equipment-degradation problems. The EPRI implementation project for on-line monitoring has demonstrated the feasibility of on-line monitoring at several participating nuclear plants. The results have been very encouraging, and substantial progress is anticipated in the coming years.

  6. DOE-EPRI On-Line Monitoring Implementation Guidelines

    SciTech Connect (OSTI)

    E. Davis, R. Bickford

    2003-01-02T23:59:59.000Z

    Industry and EPRI experience at several plants has shown on-line monitoring to be very effective in identifying out-of-calibration instrument channels or indications of equipment-degradation problems. The EPRI implementation project for on-line monitoring has demonstrated the feasability of on-line monitoring at several participating nuclear plants. The results have been very enouraging, and substantial progress is anticipated in the coming years.

  7. Inertial confinement fusion method producing line source radiation fluence

    DOE Patents [OSTI]

    Rose, Ronald P. (Peters Township, Washington County, PA)

    1984-01-01T23:59:59.000Z

    An inertial confinement fusion method in which target pellets are imploded in sequence by laser light beams or other energy beams at an implosion site which is variable between pellet implosions along a line. The effect of the variability in position of the implosion site along a line is to distribute the radiation fluence in surrounding reactor components as a line source of radiation would do, thereby permitting the utilization of cylindrical geometry in the design of the reactor and internal components.

  8. Monolithic torpedo bottle lining at Weirton Steel Corporation

    SciTech Connect (OSTI)

    Baker, R.; Griffith, E. [Weirton Steel Corp., WV (United States)

    1996-12-31T23:59:59.000Z

    In late 1992 and early 1993 Weirton Steel burned through three torpedo bottles in a three-month period. To determine the cause of the burn throughs, a thorough review of bottle maintenance practices was initiated. Upon identification of contributing factors, changes in operating practices were made. In an effort to increase bottle reliability, lining trials were initiated. Among the trials, a monolithic lining was installed and this paper will discuss results of the lining to date.

  9. TRANSPORT OF WASTE SIMULANTS IN PJM VENT LINES

    SciTech Connect (OSTI)

    Qureshi, Z

    2007-02-21T23:59:59.000Z

    The experimental work was conducted to determine whether there is a potential for waste simulant to transport or 'creep' up the air link line and contaminate the pulse jet vent system, and possibly cause long term restriction of the air link line. Additionally, if simulant creep occurred, establish operating parameters for washing down the line. The amount of the addition of flush fluids and mixer downtime must be quantified.

  10. Slow sound in lined flow ducts

    E-Print Network [OSTI]

    Auregan, Yves

    2015-01-01T23:59:59.000Z

    We consider the acoustic propagation in lined flow duct with a purely reactive impedance at the wall. This reacting liner has the capability to reduce the speed of sound, and thus to enhance the interaction between the acoustic propagation and the low Mach number flow ($M\\simeq0.3$). At the lower frequencies, there are typically 4 acoustic or hydrodynamic propagating modes, with 3 of them propagating in the direction of the flow. Above a critical frequency, there are only 2 propagating modes that all propagate in the direction of the flow. From the exact 2D formulation an approximate 1D model is developed to study the scattering of acoustic waves in a straight duct with varying wall impedance. This simple system, with a uniform flow and with a non-uniform liner impedance at the wall, permits to study the scattering between regions with different waves characteristics. Several situations are characterized to show the importance of negative energy waves, strong interactions between acoustic and hydrodynamic mod...

  11. Contingency Analysis of Cascading Line Outage Events

    SciTech Connect (OSTI)

    Thomas L Baldwin; Magdy S Tawfik; Miles McQueen

    2011-03-01T23:59:59.000Z

    As the US power systems continue to increase in size and complexity, including the growth of smart grids, larger blackouts due to cascading outages become more likely. Grid congestion is often associated with a cascading collapse leading to a major blackout. Such a collapse is characterized by a self-sustaining sequence of line outages followed by a topology breakup of the network. This paper addresses the implementation and testing of a process for N-k contingency analysis and sequential cascading outage simulation in order to identify potential cascading modes. A modeling approach described in this paper offers a unique capability to identify initiating events that may lead to cascading outages. It predicts the development of cascading events by identifying and visualizing potential cascading tiers. The proposed approach was implemented using a 328-bus simplified SERC power system network. The results of the study indicate that initiating events and possible cascading chains may be identified, ranked and visualized. This approach may be used to improve the reliability of a transmission grid and reduce its vulnerability to cascading outages.

  12. Boron-Lined Neutron Detector Measurements

    SciTech Connect (OSTI)

    Lintereur, Azaree T.; Kouzes, Richard T.; Ely, James H.; Erikson, Luke E.; Siciliano, Edward R.; Woodring, Mitchell L.

    2010-03-07T23:59:59.000Z

    PNNL-18938 Revision Radiation portal monitors used for interdiction of illicit materials at borders include highly sensitive neutron detection systems. The main reason for having neutron detection capability is to detect fission neutrons from plutonium. The currently deployed radiation portal monitors (RPMs) from Ludlum and Science Applications International Corporation (SAIC) use neutron detectors based upon 3He-filled gas proportional counters, which are the most common large neutron detector. There is a declining supply of 3He in the world, and thus, methods to reduce the use of this gas in RPMs with minimal changes to the current system designs and sensitivity to cargo-borne neutrons are being investigated. Four technologies have been identified as being currently commercially available, potential alternative neutron detectors to replace the use of 3He in RPMs. Reported here are the results of tests of a newly designed boron-lined proportional counter option. This testing measured the neutron detection efficiency and gamma ray rejection capabilities of two successive prototypes of a system manufactured by GE Reuter Stokes.

  13. Boron-Lined Neutron Detector Measurements

    SciTech Connect (OSTI)

    Lintereur, Azaree T.; Kouzes, Richard T.; Ely, James H.; Erikson, Luke E.; Siciliano, Edward R.

    2009-11-02T23:59:59.000Z

    Radiation portal monitors used for interdiction of illicit materials at borders include highly sensitive neutron detection systems. The main reason for having neutron detection capability is to detect fission neutrons from plutonium. The currently deployed radiation portal monitors (RPMs) from Ludlum and Science Applications International Corporation (SAIC) use neutron detectors based upon 3He-filled gas proportional counters, which are the most common large neutron detector. There is a declining supply of 3He in the world, and thus, methods to reduce the use of this gas in RPMs with minimal changes to the current system designs and sensitivity to cargo-borne neutrons are being investigated. Four technologies have been identified as being currently commercially available, potential alternative neutron detectors to replace the use of 3He in RPMs. Reported here are the results of tests of a newly designed boron-lined proportional counter option. This testing measured the neutron detection efficiency and gamma ray rejection capabilities of a system manufactured by Reuter Stokes.

  14. Second Line of Defense Spares Program

    SciTech Connect (OSTI)

    Henderson, Dale L.; Holmes, Aimee E.; Muller, George; Mercier, Theresa M.; Brigantic, Robert T.; Perkins, Casey J.; Cooley, Scott K.; Thorsen, Darlene E.

    2012-11-20T23:59:59.000Z

    During Fiscal Year 2012, a team from the Pacific Northwest National Laboratory (PNNL) conducted an assessment and analysis of the Second Line of Defense (SLD) Sustainability spare parts program. Spare parts management touches many aspects of the SLD Sustainability Program including contracting and integration of Local Maintenance Providers (LMP), equipment vendors, analyses and metrics on program performance, system state of health, and maintenance practices. Standardized spares management will provide better data for decisions during site transition phase and will facilitate transition to host country sustainability ownership. The effort was coordinated with related SLD Sustainability Program initiatives, including a configuration items baselining initiative, a metrics initiative, and a maintenance initiative. The spares study has also led to pilot programs for sourcing alternatives that include regional intermediate inventories and partnering agreements that leverage existing supply chains. Many partners from the SLD Sustainability program contributed to and were consulted in the course of the study. This document provides a description of the findings, recommendations, and implemented solutions that have resulted from the study.

  15. active power line: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Modeling, System Brest, Universit de 8 Reference: RGL 83-09 Subject: POWER TRANSMISSION LINES Engineering Websites Summary: Reference: RGL 83-09 Subject: POWER TRANSMISSION...

  16. EIS-0325: Schultz-Hanford Area Transmission Line Project, WA

    Broader source: Energy.gov [DOE]

    BPA proposes to construct a new 500-kilovolt (kV) transmission line in central Washington. This project would increase transmission system capacity north of Hanford.

  17. ac power lines: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    A FUNDAMENTAL characteristic construction of both generation facilities and new transmission lines, so better utilization of existing power Lehn, Peter W. 5 A new high performance...

  18. 20120131 DOE Congestion Study Clean Line Comments 2012

    Broader source: Energy.gov (indexed) [DOE]

    should encourage transmission developers to propose corridors to be 1 www.nrel.govwindresourceassessment.html 2 www.maps.platts.com Existing High Voltage Transmission Lines...

  19. A simple line wave generator using commercial explosives

    SciTech Connect (OSTI)

    Morris, John S [Los Alamos National Laboratory; Jackson, Scott I [Los Alamos National Laboratory; Hill, Larry G [Los Alamos National Laboratory

    2009-01-01T23:59:59.000Z

    We present a simple and inexpensive explosive line wave generator has been designed using commercial sheet explosive and plane wave lens concepts. The line wave generator is constructed using PETN and RDX based sheet explosive for the slow and fast components respectively. The design permits the creation of any desired line width. A series of experiments were performed on a 100 mm design, measuring the detonation arrival time at the output of the generator using a streak camera. An iterative technique was used to adjust the line wave generator's slow and fast components, so as to minimize the arrival time deviation. Designs, test results, and concepts for improvements will be discussed.

  20. A 1.3 cm Line Survey toward Orion KL

    E-Print Network [OSTI]

    Gong, Y; Thorwirth, S; Spezzano, S; Menten, K M; Walmsley, C M; Wyrowski, F; Mao, R Q; Klein, B

    2015-01-01T23:59:59.000Z

    Orion KL has served as a benchmark for spectral line searches throughout the (sub)millimeter regime. The main goal is to systematically study spectral characteristics of Orion KL in the 1.3 cm band. We carried out a spectral line survey (17.9 GHz to 26.2 GHz) with the Effelsberg-100 m telescope towards Orion KL. We find 261 spectral lines, yielding an average line density of about 32 spectral features per GHz above 3$\\sigma$. The identified lines include 164 radio recombination lines (RRLs) and 97 molecular lines. A total of 23 molecular transitions from species known to exist in Orion KL are detected for the first time in the interstellar medium. Non-metastable 15NH3 transitions are detected in Orion KL for the first time. Based on the velocity information of detected lines and the ALMA images, the spatial origins of molecular emission are constrained and discussed. A narrow feature is found in SO2 ($8_{1,7}-7_{2,6}$), possibly suggesting the presence of a maser line. Column densities and fractional abundanc...

  1. Historical Time Line and Information About the Hanford Site

    SciTech Connect (OSTI)

    Briggs Jr, David

    2001-03-07T23:59:59.000Z

    Historical time line of the Hanford Site spanning from 1940 through 1997, including photographs and other information regarding the town sites and living conditions.

  2. EIS-0317: Kangley-Echo Lake Transmission Line Project

    Broader source: Energy.gov [DOE]

    This EIS analyzes BPA's proposal to build a new transmission line to accommodate increasing demand for electricity and ensure reliability in the Puget Sound area.

  3. ,"Energy","Water","Renewable","Petroleum","Alt. Fuel",,"On-Line...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy","Water","Renewable","Petroleum","Alt. Fuel",,"On-Line Data Collection System",,"Report Period","Due In",,,"Primary","Secondary","Secondary" 2003,,,"EMS4","Environmental...

  4. The process of materials specifications for rubber lining

    SciTech Connect (OSTI)

    Earnest, R.A. [Polymeric Protective Linings, Livonia, MI (United States)

    1998-12-31T23:59:59.000Z

    Rubber is an engineered material with a long history of development, and application. The process of selecting the appropriate material for a given application is critical to the success of a lining project. A through knowledge of the process parameters for a rubber lined system will generate the information necessary to ensure maximum service life from the lining materials. Communication between the owner, applicator and manufacturer defines the process of material specification and selection. The goal of this process is to engineer the best fit for a rubber lining application.

  5. Emission Line Properties of the Large Bright Quasar Survey

    E-Print Network [OSTI]

    Karl Forster; Paul J. Green; Thomas L. Aldcroft; Marianne Vestergaard; Craig B. Foltz; Paul C. Hewett

    2000-11-20T23:59:59.000Z

    We present measurements of the optical/UV emission lines for a large homogeneous sample of 993 quasars from the Large Bright Quasar Survey. Our largely automated technique accounts for continuum breaks and galactic reddening, and we perform multicomponent fits to emission line profiles, including the effects of blended iron emission, and of absorption lines both galactic and intrinsic. Here we describe the fitting algorithm and present the results of line fits to the LBQS sample, including upper limits to line equivalent widths when warranted. The distribution of measured line parameters, principally equivalent width and FWHM, are detailed for a variety of lines, including upper limits. We thus initiate a large-scale investigation of correlations between the high energy continuum and emission lines in quasars, to be extended to complementary samples using similar techniques. High quality, reproducible measurements of emission lines for uniformly selected samples will advance our understanding of active galaxies, especially in a new era of large surveys selected by a variety of complementary methods.

  6. ac line conditioners: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of lines . ..2 Main Terasoma, Tomohide 83 Study of supplementary controls for embedded HVDC links in a AC power system. Open Access Theses and Dissertations Summary: ??This...

  7. Relativistic formulation for the Doppler-broadened line profile

    SciTech Connect (OSTI)

    Huang, Young-Sea; Chiue, Juang-Han; Huang, Yi-Chi; Hsiung, Te-Chih [Department of Physics, Soochow University, Shih-Lin, Taipei, Taiwan (China); Department of Physics, National Central University, Chung-Li, Taiwan (China)

    2010-07-15T23:59:59.000Z

    Profiles of spectral lines due to the thermal motion of light-emitting particles are formulated based on the classical and the relativistic Doppler effects, respectively. For the classical case, the well-known Doppler-broadened line profile is reproduced. For the relativistic case, the line profile obtained is asymmetrically broadened with increasing temperature. However, the peak frequency remains unshifted, in contrast to blueshifted, as has been predicted in the current literature. Reasoning is given as to why the relativistic Doppler-broadened line profile currently accepted is probably invalid.

  8. Electrical and Biological Effects of Transmission Lines: A Review.

    SciTech Connect (OSTI)

    Lee, Jack M.

    1989-06-01T23:59:59.000Z

    This review describes the electrical properties of a-c and d-c transmission lines and the resulting effects on plants, animals, and people. Methods used by BPA to mitigate undesirable effects are also discussed. Although much of the information in this review pertains to high-voltage transmission lines, information on distribution lines and electrical appliances is included. The electrical properties discussed are electric and magnetic fields and corona: first for alternating-current (a-c) lines, then for direct current (d-c).

  9. Stability and the Amorphous State

    E-Print Network [OSTI]

    Rades, T.

    2006-10-26T23:59:59.000Z

    ?? ?? ?? ?? =K 211 22111 )( wKw TgwKTgw mixTg ?+ ??+? = 13 As ?? values are not readily determinable. The commonly used version of the GT equation, which is described below for a ternary system: The Couchman-Karasz (CK) equation is similar to the GT equation... the extrudate Tg 24 Nifedipine / PVP (1:1), Solid line: CK equation based on 0.11 J/g/?C for the ?Cp of water; dashed line: GT-equation; dotted line: CK equation based on 1.38 J/g/?C for the ?Cp of water; dashed-dotted line: CK equation based on 1.94 J...

  10. A photoelastic study of the effects of surface geometry on fault movements

    E-Print Network [OSTI]

    Barber, David Williams

    1973-01-01T23:59:59.000Z

    , California, showing two en-echelon segments separated by complex zone of subsidiary faulting (after Vedder and Wallace, 1970, sheet 2). Solid lines are specific photogeologic features associated with fault- ing while dashed lines are inferred from less... lines, and os, the least compressive stress, by dashed lines. 24 stresses are higher on the two en-echelon fault segments than on the ramp. The external load is about 0. 07 bars and it i. s increasing sharply. Stored strain energy in the ramp area...

  11. Engineering and targeting glycan receptor binding of influenza A virus hemagglutinin

    E-Print Network [OSTI]

    Jayaraman, Akila

    2011-01-01T23:59:59.000Z

    The critical first step in the host infection by influenza A virus is the binding of the viral surface glycoprotein hemagglutinin (HA) to the sialylated glycan receptors terminated by N-acetyineuraminic acid (Neu5Ac) ...

  12. Identification of Novel Small Molecule Inhibitors of Core-Binding Factor Dimerization by Computational

    E-Print Network [OSTI]

    Lilien, Ryan

    of a ligand database into the binding site of the protein. Ligands are then ranked by the number and quality (IIS-9906790, EIA- 0102710, EIA-0102712, EIA-9818299, EIA-9802068, and EIA-0305444). Author's current

  13. Conformational Transitions upon Ligand Binding: Holo-Structure Prediction from Apo Conformations

    E-Print Network [OSTI]

    de Groot, Bert

    Conformational Transitions upon Ligand Binding: Holo- Structure Prediction from Apo Conformations Daniel Seeliger, Bert L. de Groot* Computational Biomolecular Dynamics Group, Max design. Hence, if only an unbound (apo) structure is available distinct from the ligand

  14. Networks of Coupled Rotators: Relationship between Structures and Internal Dynamics in Metal-Binding Proteins.

    E-Print Network [OSTI]

    -Binding Proteins. Applications to apo- and holo-Calbindin Anne Dhulesia, Daniel Abergel,* and Geoffrey Bodenhausen, France Received October 17, 2006; E-mail: daniel.abergel@ens.fr Abstract: This article presents

  15. Dynamical bond cooperativity enables very fast and strong binding between sliding surfaces

    E-Print Network [OSTI]

    Trømborg, Jørgen Kjoshagen

    2015-01-01T23:59:59.000Z

    Cooperative binding affects many processes in biology, but it is commonly addressed only in equilibrium. In this work we explore dynamical cooperativity in driven systems, where the cooperation occurs because some of the bonds change the dynamical response of the system to a regime where the other bonds become active. To investigate such cooperativity we study the frictional binding between two flow driven surfaces that interact through a large population of activated bonds. In particular, we study systems where each bond can have two different modes: one mode corresponds to a fast forming yet weak bond, and the other is a strong yet slow forming bond. We find considerable cooperativity between both types of bonds. Under some conditions the system behaves as if there were only one binding mode, corresponding to a strong and fast forming bond. Our results may have important implications on the friction and adhesion between sliding surfaces containing complementary binding motifs, such as in the case of cells b...

  16. Chromosome-Biased Binding and Gene Regulation by the Caenorhabditis elegans DRM Complex

    E-Print Network [OSTI]

    Tabuchi, Tomoko M.

    DRM is a conserved transcription factor complex that includes E2F/DP and pRB family proteins and plays important roles in development and cancer. Here we describe new aspects of DRM binding and function revealed through ...

  17. Identification and analysis of hepatitis C virus NS3 helicase inhibitors using nucleic acid binding assays

    E-Print Network [OSTI]

    Mukherjee, Sourav; Hanson, Alica M.; Shadrick, William R.; Ndjomou, Jean; Sweeney, Noreena L.; Hernadez, John J.; Bartczak, Diana; Li, Kelin; Frankowski, Kevin J.; Heck, Julie A.; Arnold, Leggy A.; Schoenen, Frank; Frick, David N,

    2012-06-27T23:59:59.000Z

    Typical assays used to discover and analyze small molecules that inhibit the hepatitis C virus (HCV) NS3 helicase yield few hits and are often confounded by compound interference. Oligonucleotide binding assays are examined ...

  18. Rational Design of New Binding Specificity by Simultaneous Mutagenesis of Calmodulin and a Target Peptide

    E-Print Network [OSTI]

    Green, David F.

    Calcium-saturated calmodulin (CaM) binds and influences the activity of a varied collection of target proteins in most cells. This promiscuity underlies the role of CaM as a shared participant in calcium-dependent signal ...

  19. Solution Structure of the cGMP Binding GAF Domain fromPhosphodiesteras...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of cGMP through its regulation of cGMP hydrolysis. Hydrolytic activity of the C-terminal catalytic domain is increased by cGMP binding to the N-terminal GAF A domain. We...

  20. Analysis of variation at transcription factor binding sites in Drosophila and humans

    E-Print Network [OSTI]

    Spivakov, Mikhail

    Background: Advances in sequencing technology have boosted population genomics and made it possible to map the positions of transcription factor binding sites (TFBSs) with high precision. Here we investigate TFBS variability ...

  1. On the electrostatic component of protein-protein binding free energy

    E-Print Network [OSTI]

    Talley, Kemper; Ng, Carmen; Shoppell, Michael; Kundrotas, Petras J.; Alexov, Emil

    2008-11-05T23:59:59.000Z

    of the electrostatic component of binding free energy (DeltaDeltaGel) with respect with different force fields (Charmm, Amber, and OPLS), different values of the internal dielectric constant, and different presentations of molecular surface (different values...

  2. Quantitative estimates on the enhanced binding for the Pauli-Fierz operator

    SciTech Connect (OSTI)

    Barbaroux, Jean-Marie; Linde, Helmut; Vugalter, Semjon [Centre de Physique Theorique, Luminy Case 907, 13288 Marseille Cedex 9 (France); Departement de Mathematiques, Universite du Sud-Toulon-Var, Avenue de l'Universite, 83957 La Garde Cedex (France); Facultad de Fisica, P. Universidad Catolica de Chile, Casilla 306, Santiago 22 (Chile); Mathematisches Institut, Ludwig-Maximilians-Universitaet Muenchen, Theresienstrasse 39, 80333 Munich (Germany); Institut fuer Analysis, Dynamik und Modelierung, Universitaet Stuttgart, Stuttgart (Germany)

    2005-12-15T23:59:59.000Z

    For a quantum particle interacting with a short-range potential, we estimate from below the shift of its binding threshold, which is due to the particle interaction with a quantized radiation field.

  3. 2D IR spectroscopy and computational modeling : application to protein folding and binding

    E-Print Network [OSTI]

    Ganim, Ziad

    2010-01-01T23:59:59.000Z

    In this thesis, dynamics experiments are developed that can be used to study protein conformational changes such as folding and binding. Every functional motion of a protein is inextricably linked to conformational dynamics. ...

  4. Visualizing KcsA Conformational Changes upon Ion Binding by Infrared Spectroscopy and Atomistic Modeling

    E-Print Network [OSTI]

    Stevenson, Paul

    The effect of ion binding in the selectivity filter of the potassium channel KcsA is investigated by combining amide I Fourier-transform infrared spectroscopy with structure-based spectral modeling. Experimental difference ...

  5. Control of HslUV protease function by nucleotide binding and hydrolysis

    E-Print Network [OSTI]

    Yakamavich, Joseph Andrew

    2008-01-01T23:59:59.000Z

    Many proteins act as molecular machines, using the power of nucleotide binding and hydrolysis to drive conformational changes in themselves and their target substrates. Like other AAA+ proteases, HslUV recognizes, unfolds, ...

  6. Stabilizing the cystic fibrosis transmembrane conductance regulator (CFTR) by nucleotide derivative binding to promote proper folding

    E-Print Network [OSTI]

    Smith, Ryan Craig

    2013-02-22T23:59:59.000Z

    Seventy percent of people who suffer from cystic fibrosis have a cystic fibrosis transmembrane conductance regulator gene on chromosome 7 that contains a three base-pair deletion of phenylalanine at position 508, in a nucleotide binding domain...

  7. Nucleotide Binding and Conformational Switching in the Hexameric Ring of a AAA+ Machine

    E-Print Network [OSTI]

    Stinson, Benjamin Michael

    ClpX, a AAA+ ring homohexamer, uses the energy of ATP binding and hydrolysis to power conformational changes that unfold and translocate target proteins into the ClpP peptidase for degradation. In multiple crystal structures, ...

  8. Structures of Human Pumilio with Noncognate RNAs Reveal Molecular Mechanisms for Binding Promiscuity

    SciTech Connect (OSTI)

    Gupta,Y.; Nair, D.; Wharton, R.; Aggarwal, A.

    2008-01-01T23:59:59.000Z

    Pumilio is a founder member of the evolutionarily conserved Puf family of RNA-binding proteins that control a number of physiological processes in eukaryotes. A structure of human Pumilio (hPum) Puf domain bound to a Drosophila regulatory sequence showed that each Puf repeat recognizes a single nucleotide. Puf domains in general bind promiscuously to a large set of degenerate sequences, but the structural basis for this promiscuity has been unclear. Here, we describe the structures of hPum Puf domain complexed to two noncognate RNAs, CycBreverse and Puf5. In each complex, one of the nucleotides is ejected from the binding surface, in effect, acting as a 'spacer.' The complexes also reveal the plasticity of several Puf repeats, which recognize noncanonical nucleotides. Together, these complexes provide a molecular basis for recognition of degenerate binding sites, which significantly increases the number of mRNAs targeted for regulation by Puf proteins in vivo.

  9. Nucleotide binding and conformational switching in the hexameric ring of a AAA+ machine

    E-Print Network [OSTI]

    Stinson, Benjamin M. (Benjamin Michael)

    2015-01-01T23:59:59.000Z

    ATP-powered proteases enforce protein quality-control and regulation in all domains of life. ClpX, a AAA+ ring homohexamer, uses the energy of ATP binding and hydrolysis to power conformational changes that unfold and ...

  10. Structural and functional consequences of platinum anticancer drug binding to free and nucleosomal DNA

    E-Print Network [OSTI]

    Todd, Ryan Christopher, 1981-

    2010-01-01T23:59:59.000Z

    Cisplatin, carboplatin, and oxaliplatin are three FDA-approved members of the platinum anticancer drug family. These compounds induce apoptosis in tumor cells by binding to nuclear DNA, forming a variety of adducts, and ...

  11. apobec3g binding site: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ser-142 (14). Ser-129 is believed to be a substrate for glycogen synthase kinase 3 Brownfield, Mark S. 73 Similarity Analysis of Protein Binding Sites: A Generalization of the...

  12. acyl-coa binding protein: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ser-142 (14). Ser-129 is believed to be a substrate for glycogen synthase kinase 3 Brownfield, Mark S. 131 A High-Throughput Solid-Phase Microplate Protein-Binding Assay to...

  13. ap-2alpha binding site: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ser-142 (14). Ser-129 is believed to be a substrate for glycogen synthase kinase 3 Brownfield, Mark S. 53 Similarity Analysis of Protein Binding Sites: A Generalization of the...

  14. acyl-coa binding proteins: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ser-142 (14). Ser-129 is believed to be a substrate for glycogen synthase kinase 3 Brownfield, Mark S. 131 A High-Throughput Solid-Phase Microplate Protein-Binding Assay to...

  15. Effects of dietary fiber and carcinogen on fatty acid binding protein expression in exfoliated colonocytes

    E-Print Network [OSTI]

    Clark, Amy Eunice

    1997-01-01T23:59:59.000Z

    EFFECTS OF DIETARY FIBER AND CARCINOGEN ON FATTY ACID BINDING PROTEIN EXPRESSION IN EXFOLIATED COLONOCYTES A Thesis by AMY EUNICE CLARK Submitted to the Office of Gmduate Studies of Texas A&M University in partial fulfillment...) Bryan H. Johnson (Head of Department) August 1997 Major Subject: Nutrition ABSTRACT Effects of Dietary Fiber and Carcinogen on Fatty Acid Binding Protein Expression in Exfoliated Colonocytes. (August 1997) Amy Eunice Clark, B. S. , Texas A...

  16. Ab-initio calculation of the ${}^6Li$ binding energy with the Hybrid Multideterminant scheme

    E-Print Network [OSTI]

    Giovanni Puddu

    2010-06-09T23:59:59.000Z

    We perform an ab-initio calculation for the binding energy of ${}^6Li$ using the CD-Bonn 2000 NN potential renormalized with the Lee-Suzuki method. The many-body approach to the problem is the Hybrid Multideterminant method. The results indicate a binding energy of about $31 MeV$, within a few hundreds KeV uncertainty. The center of mass diagnostics are also discussed.

  17. Internal binding sites for MSH: Analyses in wild-type and variant Cloudman melanoma cells

    SciTech Connect (OSTI)

    Orlow, S.J.; Hotchkiss, S.; Pawelek, J.M. (Yale Univ. School of Medicine, New Haven, CT (USA))

    1990-01-01T23:59:59.000Z

    Cloudman S91 mouse melanoma cells express both external (plasma membrane) and internal binding sites for MSH. Using 125I-beta melanotropin (beta-MSH) as a probe, we report here an extensive series of studies on the biological relevance of these internal sites. Cells were swollen in a hypotonic buffer and lysed, and a particulate fraction was prepared by high-speed centrifugation. This fraction was incubated with 125I-beta-MSH with or without excess nonradioactive beta-MSH in the cold for 2 hours. The material was then layered onto a step-wise sucrose gradient and centrifuged; fractions were collected and counted in a gamma counter or assayed for various enzymatic activities. The following points were established: (1) Specific binding sites for MSH were observed sedimenting at an average density of 50% sucrose in amelanotic cells and at higher densities in melanotic cells. (2) These sites were similar in density to those observed when intact cells were labeled externally with 125I-beta-MSH and then warmed to promote internalization of the hormone. (3) Most of the internal binding sites were not as dense as fully melanized melanosomes. (4) In control experiments, the MSH binding sites were not found in cultured hepatoma cells. (5) Variant melanoma cells, which differed from the wild-type in their responses to MSH, had reduced expression of internal binding sites even though their ability to bind MSH to the outer cell surface appeared normal. (MSH-induced responses included changes in tyrosinase, dopa oxidase, and dopachrome conversion factor activities, melanization, proliferation, and morphology.) (6) Isobutylmethylxanthine, which enhanced cellular responsiveness to MSH, also enhanced expression of internal binding sites. The results indicate that expression of internal binding sites for MSH is an important criterion for cellular responsiveness to the hormone.

  18. Structural basis for the evolutionary inactivation of Ca[superscript 2+] binding to synaptotagmin 4

    SciTech Connect (OSTI)

    Dai, Han; Shin, Ok-Ho; Machius, Mischa; Tomchick, Diana R.; Südhof, Thomas C.; Rizo, Josep (U. of Texas-SMED)

    2010-11-16T23:59:59.000Z

    The neuronal protein synaptotagmin 1 functions as a Ca{sup 2+} sensor in exocytosis via two Ca{sup 2+}-binding C{sub 2} domains. The very similar synaptotagmin 4, which includes all the predicted Ca{sup 2+}-binding residues in the C{sub 2}B domain but not in the C{sub 2}A domain, is also thought to function as a neuronal Ca{sup 2+} sensor. Here we show that, unexpectedly, both C{sub 2} domains of fly synaptotagmin 4 exhibit Ca{sup 2+}-dependent phospholipid binding, whereas neither C{sub 2} domain of rat synaptotagmin 4 binds Ca{sup 2+} or phospholipids efficiently. Crystallography reveals that changes in the orientations of critical Ca{sup 2+} ligands, and perhaps their flexibility, render the rat synaptotagmin 4 C{sub 2}B domain unable to form full Ca{sup 2+}-binding sites. These results indicate that synaptotagmin 4 is a Ca{sup 2+} sensor in the fly but not in the rat, that the Ca{sup 2+}-binding properties of C{sub 2} domains cannot be reliably predicted from sequence analyses, and that proteins clearly identified as orthologs may nevertheless have markedly different functional properties.

  19. Binding of He{sub n}V clusters to ?-Fe grain boundaries

    SciTech Connect (OSTI)

    Tschopp, M. A., E-mail: mark.a.tschopp.civ@mail.mil [U.S. Army Research Laboratory, Aberdeen Proving Ground, Maryland 21005 (United States); Gao, F. [Pacific Northwest National Laboratory, Richland, Washington 99352 (United States); Solanki, K. N. [Arizona State University, Tempe, Arizona 85287 (United States)

    2014-06-21T23:59:59.000Z

    The objective of this research is to explore the formation/binding energetics and length scales associated with the interaction between He{sub n}V clusters and grain boundaries in bcc ?-Fe. In this work, we calculated formation/binding energies for 1–8 He atoms in a monovacancy at all potential grain boundary (GB) sites within 15?Å of the ten grain boundaries selected (122106 simulations total). The present results provide detailed information about the interaction energies and length scales of 1–8 He atoms with grain boundaries for the structures examined. A number of interesting new findings emerge from the present study. First, the ?3(112) “twin” GB has significantly lower binding energies for all He{sub n}V clusters than all other boundaries in this study. For all grain boundary sites, the effect of the local environment surrounding each site on the He{sub n}V formation and binding energies decreases with an increasing number of He atoms in the He{sub n}V cluster. Based on the calculated dataset, we formulated a model to capture the evolution of the formation and binding energy of He{sub n}V clusters as a function of distance from the GB center, utilizing only constants related to the maximum binding energy and the length scale.

  20. Binding proteins for growth hormone and prolactin in rabbit kidney cytosol

    SciTech Connect (OSTI)

    Herington, A.C.; Stevenson, J.L.; Ymer, S.I. (Prince Henry's Hospital, Melbourne (Australia))

    1988-09-01T23:59:59.000Z

    Two soluble, receptor-like binding proteins with apparent somatotrophic (growth hormone (GH)) and lactogenic (prolactin (PRL)) specificities, respectively, and that are present in rabbit kidney cytosol have now been examined in more detail using specific GH receptor and PRL receptor monoclonal antibodies (MAb). Gel chromatography of {sup 125}I-labeled human GH ({sup 125}I-hGH) kidney cytosol complexes in the absence of these MAbs revealed two specifically bound regions of radioactivity at molecular weights (MW) of {approximately}120,000 and {approximately}60,000, which are similar in size to complexes formed by the native GH receptor of rabbit liver cytosol and the PRL receptor of mammary gland. Co-incubation with GH-receptor MAb inhibited {sup 125}I-hGH binding only to the higher MW (120,000) species, whereas the PRL-receptor MAb inhibited only the lower MW (60,000) species, thus establishing definitively the hormonal specificities of the two binding proteins. The presence of both GH- and PRL-specific binding subunits in cytosol was confirmed using covalent cross-linking techniques. No GH binding protein was detected in kidney membranes. The presence of naturally soluble, receptor-like binding proteins for GH and PRL in kidney cytosol preparations raises the possibility of their playing a role in the intracellular regulation of kidney function and/or metabolism.